LOCUS       DAHHOJ010000001        73003 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.001, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000001 DAHHOJ010000000
VERSION     DAHHOJ010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 73003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.001"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            322..1623
                     /locus_tag="NBH32_000001"
     CDS             322..1623
                     /locus_tag="NBH32_000001"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HCD1513648.1"
                     /translation="MRMIDIIEKKRDGHTLTTEEINFFIDGYVKGDIPDYQASSLAMA
                     IYFQDMNDDERAALTMAMVNSGDMIDLSDIKGVKVDKHSTGGVGDTTTLVLAPLVAAV
                     DVPVAKMSGRGLGHTGGTIDKLEAIDGFHVEIDEATFVKLVNENKVAVVGQSGNLTPA
                     DKKLYALRDVTGTVNSIPLIASSIMSKKIAAGADAIVLDVKTGSGAFMKTLEDAEALA
                     HAMVRIGNNVGRNTMAIISDMNQPLGRAIGNALELQEAIDTLKGHGPKDLTELVLTLG
                     SQMVVLANKAETLEEARALLIEAIDSGAALEKFKTFIKNQGGDETVIDHPERLPQAQY
                     QIEYKAKKSGYVTELVSNDIGVASMMLGAGRLTKEDDIDLAVGIVLNKKIGDKVEEGE
                     SLLTIHSNRQDVDDVVKKLDSSITIADHVVSPTLIHKIITE"
     gene            1637..1987
                     /locus_tag="NBH32_000002"
     CDS             1637..1987
                     /locus_tag="NBH32_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513649.1"
                     /translation="MNILRYLLGIGFSVIGVLHFTRERQFRNIIPKCLPLRKTAVLVT
                     GIFEILFGLALLIKRPSQCLKNTINLFLLAVFPANIYMAVKKLPLGDKQLPTWQLYLR
                     LPMQFVLMGLIKKL"
     gene            complement(2139..2609)
                     /locus_tag="NBH32_000003"
     CDS             complement(2139..2609)
                     /locus_tag="NBH32_000003"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCD1513650.1"
                     /translation="MTKMNVESFNLDHTKVVAPFIRLAGTMEGLNGDVIHKYDIRFKQ
                     PNKEHMDMPGLHSLEHLMAENIRNHSDKVVDLSPMGCQTGFYVSFIDHDNYDDVLNIV
                     EATLNDVLKATEVPACNEVQCGWAASHSLEGAKTIAQAFLDKRNEWHDVFGTGK"
     gene            2920..4104
                     /locus_tag="NBH32_000004"
     CDS             2920..4104
                     /locus_tag="NBH32_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HCD1513651.1"
                     /translation="MSEKQQILDYIETNKYSYIEISHRIHERPELGNEEIFASRTLID
                     RLKEHDFEIETEIAGHATGFIATYDSGLDGPAIGFLAEYDALPGLGHACGHNIIGTAS
                     VLGAIGLKQVIDQIGGKVVVLGCPAEEGGENGSAKASYVKAGVIDQIDIALMIHPGNE
                     TYKTIDTLAVDVLDVKFYGKSAHASENADEALNALDAMISYFNGVAQLRQHIKKDQRV
                     HGVILDGGKAANIIPDYTHARFYTRAMTRKELDILTEKVNQIARGAAIQTGCDYEFGP
                     IQNGVNEFIKTPKLDDLFAKYAEEVGEAVIDDDFGYGSTDTGNVSHVVPTIHPHIKIG
                     SRNLVGHTHRFREAAASVHGDEALIKGAKIMALMGLELITNQDVYQDIIEEHAHLKGN
                     GK"
     gene            4104..5297
                     /locus_tag="NBH32_000005"
     CDS             4104..5297
                     /locus_tag="NBH32_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HCD1513652.1"
                     /translation="MTNNGNEPNLTLSDLYDKDVVYTSRPSYISNPWLKPDEHQSNFL
                     TGRELLIANQLPVIVHEASATDKLHQLFQVIGKDVPNSIYTFNNQQSYENLIKQLAHK
                     ENKKIYFQYIHDETILNQQYYALDKTLFVALNNKARIPEWTNGKFLPKRKVVKIEQFE
                     NEIKNWEFPFVIKPGDDLPTAGGYGVMICYHDADLQKAITRIKEATAETNSLIIEQKI
                     EEKANYCVQFAYSESLGIQYLGAATQLTDKYGFYNGNENTTNVPEHVIEAGRQIMENG
                     VNQGFFGVAGFDLLVDEDDNVYAIDLNFRQNGSTSMLLLANELNSGYQKFYSYHSKGD
                     NTHFFNTILKYVKEGSLYPLSYYDGDWYGEDKVKSRFGCIWHGDSKETVLENERAFLA
                     ELEHY"
     gene            5696..6367
                     /locus_tag="NBH32_000006"
     CDS             5696..6367
                     /locus_tag="NBH32_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2750 domain-containing protein"
                     /protein_id="HCD1513653.1"
                     /translation="MSYKAYPFFRDILINECIYFASKNKKLVRLNYKSEAYVGVWTEE
                     SVAVSFLTSRDIPFDKVVKMDVDRFATYELDELFDEQDHIIMNQTMEEEGHLLNVVAV
                     TQEVMTELDKIRIKEFVQDVAKYDEVYGLTKKGSKQFILISENDSDEKKPHIMPVWSI
                     KNRALKVRDEDFEECDLITIEGSVFGEWLDELRDDHKAVAIDLKTGVVGTIVSAQKLS
                     NELTF"
     gene            complement(6497..7300)
                     /gene="coaW"
                     /locus_tag="NBH32_000007"
     CDS             complement(6497..7300)
                     /gene="coaW"
                     /locus_tag="NBH32_000007"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II pantothenate kinase"
                     /protein_id="HCD1513654.1"
                     /translation="MKVGIDAGGTLIKIVQEQDNQRTFKTELTKNIDQVVEWLNQQQI
                     EKLCLTGGNAGVIAENINIPAQIFVEFDAASQGLGILLKEQGHDLADYIFANVGTGTS
                     LHYFDGQSQRRVGGIGTGGGMIQGLGYLLSQITDYKQLTDMAQHGDRNTIDLKVRHIY
                     KDTEPPIPGDLTAANFGHVLHHLDADFTPSNKLAAVIGVVGEVVTTMAITVAREFKTE
                     NIVYIGSSFHNNALLRKVVEDYTVLRGCKPYYVENGAFSGAIGALYLGK"
     gene            7724..8584
                     /locus_tag="NBH32_000008"
     CDS             7724..8584
                     /locus_tag="NBH32_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1513655.1"
                     /translation="MAKELCFEGITLKAFDEQYRSAINDFDLNERQQIYSSLPKEVID
                     DAINDADRIANVAINDKNEVVGFFVLHRYYQHEGYDTPENVVYIRSLSINEKYQGFGY
                     GTKIMMSLPQYVQGVFPDFNHLYLVVDAENDNAWNLYERAGFMHTATKEEGPIGKERL
                     YYLDLDSKHVSSLKLEEESRSEVTNVHIINLMIDGQKVGFIALEQIGERMNIAAIEVD
                     KSYRFNGIGSSALRQLPTYLRKNYDNLNVITMILFGENNDFKPLCLNSNFVEIEQTDD
                     YVVFEKYLNY"
     gene            8696..9226
                     /locus_tag="NBH32_000009"
     CDS             8696..9226
                     /locus_tag="NBH32_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000701491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HCD1513656.1"
                     /translation="MKIQDYTKQMVDEKSFIDMAYTLLNDKGETMNLYDIIDEFRALG
                     DYEYEEIETRVVQFYTDLNTDGRFLNVGENLWGLRDWYSVDDIEEKIAPTIQKFDILD
                     ADDEEDQNLKLLGEDELDDDDDIPAQTDDQEELNDPEDEQVEEEINHSDIVIEEDEDE
                     LDEDEEDFEDEEDFKA"
     gene            9562..11172
                     /locus_tag="NBH32_000010"
     CDS             9562..11172
                     /locus_tag="NBH32_000010"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HCD1513657.1"
                     /translation="MTKFIFVTGGVVSSLGKGITASSLGRLLKDRGLNVTIQKFDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKFSNVTAGKVYSHVLKKE
                     RRGDYLGGTVQVIPHITNEIKERLLLAGESTNADVVITEIGGTTGDIESLPFIEAIRQ
                     IRSDLGRENVMYVHCTLLPYIKAAGEMKTKPTQHSVKELRGLGIQPDLIVVRTEYEMT
                     QDLKDKIALFCDINKESVIECRDADSLYEIPLQLSQQNMDDIVIKRLQLNAKYETQLD
                     EWKQLLDIVNNLDGKITIGLVGKYVSLQDAYLSVVESLKHAGYPFAKDIDIRWIDSSE
                     VTDENAAEYLADVDGILVPGGFGFRASEGKISAIKYARENNVPFFGICLGMQLATVEF
                     SRNVLGLEGAHSAELDPATPYPIIDLLPEQKDIEDLGGTLRLGLYPCSIKEGTLAQDV
                     YGKAEIEERHRHRYEFNNDYREQLEANGMVISGTSPDGRLVEMVEIPTNDFFIACQFH
                     PEFLSRPNRPHPIFKSFIEASLKYQQNK"
     gene            complement(11281..11802)
                     /locus_tag="NBH32_000011"
     CDS             complement(11281..11802)
                     /locus_tag="NBH32_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2529 domain-containing protein"
                     /protein_id="HCD1513658.1"
                     /translation="MSKILNTQLIGIFNRLEKQSLEIQMAAQCLIQAIGGEGYVYVKG
                     YDDLQFFESFILHGDERLKSSRKLDAIKDFKEIDSTDRVLLFAPFYNDQVALDIQKLI
                     DLDIDVVLISNKPKTDDFPDHLVHFIDLSTPRPIVYTEDYDKIVQPHAMALNYVYYDI
                     YTQMIEMTRDLEL"
     gene            12020..12880
                     /locus_tag="NBH32_000012"
     CDS             12020..12880
                     /locus_tag="NBH32_000012"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002468309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HCD1513659.1"
                     /translation="MPLVSMKEMLIDAKENGYAVGQYNINNLEFTQAILEASQEENAP
                     VILGVSEGAARYMSGFYTIVKMVEGLMHDLNITIPVAIHLDHGSSFEKCKEAIDAGFT
                     SVMIDASHSPFEENVATTKKVVEYAHEKGVSVEAELGTVGGQEDDVVADGIIYADPKE
                     CQELVEKTGIDALAPALGSVHGPYKGEPKLGFKEMEEIGLSTGLPLVLHGGTGIPTKD
                     IQKAIPFGTAKINVNTENQIASAKAVRDVLNNDKEVYDPRKYLGPAREAIKETVKGKI
                     KEFGTSNRAK"
     gene            13346..14605
                     /locus_tag="NBH32_000013"
     CDS             13346..14605
                     /locus_tag="NBH32_000013"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1513660.1"
                     /translation="MAQEVIKIRGGRTLNGEVNISGAKNSAVAIIPATLLAQGHVKLE
                     GLPQISDVKTLVSLLEDLNIKASLNGTELEVDTTEIQNAALPNNKVESLRASYYMMGT
                     MLGRFKKCVIGLPGGCPLGPRPIDQHIKGFKALGAEIDESSTTSMKIEAKELKGAHIF
                     LDMVSVGATINIMLAAVYATGQTVIENAAKEPEVVDVANFLTSMGANIKGAGTSTIKI
                     NGVKELHGSEYQVIPDRIEAGTYMCIAAACGENVILNNIVPKHVETLTAKFSELGVNV
                     DVRDERIRINNNAPYQFVDIKTLVYPGFATDLQQPITPLLFMANGPSFVTDTIYPERF
                     KHVEELKRMGANIEVDEGTATIKPSTLHGAEVYASDLRAGACLIIAGLIAEGVTTIYN
                     VKHIYRGYTDIVEHLKALGADIWTETV"
     gene            14694..15029
                     /locus_tag="NBH32_000014"
     CDS             14694..15029
                     /locus_tag="NBH32_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1513661.1"
                     /translation="MEVCPYLEETFKILGRSWNGLIINYLSRCNDCSAHFSDMKRDLK
                     TITPRALSLKLSELAQWELVEKQIISTSPVQIIYVLTEKGKALAEALHPIEAWAQSYV
                     DLTDQRTAK"
     gene            15267..16694
                     /locus_tag="NBH32_000015"
     CDS             15267..16694
                     /locus_tag="NBH32_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD1513662.1"
                     /translation="MRDYTKQYINGEWVESNSNETIEVINPATEEVIGKVAKGNKADV
                     DKAVEAADDVYLEFRHTSVKERQALLDKIVKEYENRKDDIVQAITDELGAPLSLSERV
                     HYQMGLNHFVAARDALDNYEFEERRGDDLVVKEAIGVSGLITPWNFPTNQTSLKLAAA
                     FAAGSPVVLKPSEETPFAAVILAEIFDKVGVPKGVFNLVNGDGAGVGNPLSEHPKVRM
                     MSFTGSGPTGSKIMEKAAKYFKKVSLELGGKSPYIVLDDVDIKEAAKATTGKVVNNTG
                     QVCTAGTRVLVPNKIKDAFLAELKEQFSQVRVGNPREDGTQVGPIISKKQFDQVQNYI
                     NKGIEEGAELFYGGPGKPEGLEKGYFARPTIFINVDNQMTIAQEEIFGPVMSVITYND
                     LDEAIQIANDTKYGLAGYVIGKNKETLHKVARSIEAGTVEINEAGRKPDLPFGGYKQS
                     GLGREWGDYGIEEFLEVKSIAGYFK"
     gene            16941..18257
                     /gene="rho"
                     /locus_tag="NBH32_000016"
     CDS             16941..18257
                     /gene="rho"
                     /locus_tag="NBH32_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCD1513663.1"
                     /translation="MPERERTSPQYESFHELYKNYTTKELTQKAKTLKLTNYSKLNKK
                     ELVLAIMEAQMEKDGNYYMEGILDDIQPDGYGFLRTVNYSKGEKDIYISASQIRRFEI
                     KRGDKVTGKVRKPKDNEKYYGLLQVDFVNDHNAEEVKKRPHFQALTPLYPDERIKLET
                     ETQNYSTRIMDLVTPIGLGQRGLIVAPPKAGKTSLLKEIANAISTNKPDAKLFILLVG
                     ERPEEVTDLERSVEAAEVVHSTFDEPPEHHVKVAELLLERAKRLVEIGEDVIILMDSI
                     TRLARAYNLVIPPSGRTLSGGLDPASLHKPKAFFGAARNIEAGGSLTILATALVDTGS
                     RMDDMIYEEFKGTGNMELHLDRKLSERRIFPAIDIGRSSTRKEELLISKSELDTLWQL
                     RNLFTDSTDFTERFIRKLKRSKNNEDFFKQLQKSAEESTKTGRPII"
     gene            18375..18629
                     /locus_tag="NBH32_000017"
     CDS             18375..18629
                     /locus_tag="NBH32_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HCD1513664.1"
                     /translation="MKQGIHPEYHQVIFLDTTTNFKFLSGSTKTSSEMMEWEDGKEYP
                     VIRLDISSDSHPFYTGRQKFAAADGRVERFNKKFGLKSNN"
     gene            18975..19574
                     /locus_tag="NBH32_000018"
     CDS             18975..19574
                     /locus_tag="NBH32_000018"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCD1513665.1"
                     /translation="MYETYHSGWIECITGSMFSGKSEELIRRLRRGIYAKQKVVVFKP
                     AIDDRYHKEKVVSHNGNAIEAINISKASEIMTHDLTNVDVIGIDEVQFFDDEIVSIVE
                     KLSADGHRVIVAGLDMDFRGEPFEPMPKLMAVSEQVTKLQAVCAVCGSSSSRTQRLIN
                     GKPAKIDDPIILVGANESYEPRCRAHHIVAPSDNNKEEL"
     gene            19575..20651
                     /gene="prfA"
                     /locus_tag="NBH32_000019"
     CDS             19575..20651
                     /gene="prfA"
                     /locus_tag="NBH32_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCD1513666.1"
                     /translation="MFDQLDIVEERYEQLNELLSDPDVVNDSDKLRKYSKEQADLQKT
                     VDVYRNYKAKKEELADIEEMLSETDDKEEVEMLKEESNGIKAELPNLEEELKILLIPK
                     DPNDDKDVIVEIRAAAGGDEAAIFAGDLMRMYSKYAESQGFKTEIVEASESDHGGYKE
                     ISFSVSGNGAYSKLKFENGAHRVQRVPETESGGRIHTSTATVAVLPEVEDVEIEIRNE
                     DLKIDTYRSSGAGGQHVNTTDSAVRITHLPTGVIATSSEKSQIQNREKAMKVLKARLY
                     DMKVQEEQQKYASQRKSAVGTGDRSERIRTYNYPQSRVTDHRIGLTLQKLGQIMEGHL
                     EEIIDALTLSEQTDKLKELNNGEL"
     gene            20638..21474
                     /gene="prmC"
                     /locus_tag="NBH32_000020"
     CDS             20638..21474
                     /gene="prmC"
                     /locus_tag="NBH32_000020"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCD1513667.1"
                     /translation="MVNYKEKLDEAIHLTQQKGFEQTRAEWLMLDVFQWTRTDFVVHM
                     HDDMPKAMIMKFDLALQRMLLGEPIQYIVGFASFYGRTFDVNSNCLIPRPETEEVMLH
                     FLQQLEDDATIVDIGTGSGVLAITLKCEKPDLNVIATDISLEAMNMARNNAEKHQSQI
                     QFLTGDALKPLINEGIKLNGLISNPPYIDEKDMVTMSPTVTRFEPHQALFADNHGYAI
                     YESIIEDLPHVMEKGSPVVFEIGYNQGEALKSIILNKFPDKKIDIIKDINGHDRIVSF
                     KW"
     gene            21558..22604
                     /locus_tag="NBH32_000021"
     CDS             21558..22604
                     /locus_tag="NBH32_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCD1513668.1"
                     /translation="MDTKIWDVREYNEDLQQYPKINEIKDIVLNGGLIGLPTETVYGL
                     AANATDEEAVAKIYEAKGRPSDNPLIVHIHSKDQLKDFTYILDAPVEKLMQAFWPGPI
                     SFILPLKPGYLCRKVSGGLSSVAVRMPSHSVGRQLLQIINEPLAAPSANLSGRPSPTT
                     FNHVYQDLNGRIDGIVQAEQSEEGLESTVLDCTSFPYKIARPGSITAAMITDILPNSI
                     THADYNDTEQPIAPGMKYKHYSPNTPLTIITDIESKIGNDGKDWSSIAFIVPSNKVAF
                     IPSEAQFIQLCQDDNDVKQASHNLYDVLHSLDENENISAAYIYGFELNDNTEAIMNRM
                     LKAAGNHIVKGCEL"
     gene            22601..23020
                     /locus_tag="NBH32_000022"
     CDS             22601..23020
                     /locus_tag="NBH32_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight protein arginine
                     phosphatase"
                     /protein_id="HCD1513669.1"
                     /translation="MKILFVCTGNTCRSPLAESIAKEVMPNHQFESRGIFAVNNQGVS
                     NYVEDIVEEHHLAETTLSQQFTEADLKADIILTMSYSHKELIEAQFGLQNHVFTLHEY
                     VKEAGEVIDPYGGTKEMYVHTYEELVSLILKLKDIIC"
     gene            23127..23651
                     /locus_tag="NBH32_000023"
     CDS             23127..23651
                     /locus_tag="NBH32_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HCD1513670.1"
                     /translation="MKDLTMLLDELKDMSFFNKGDICLIGCSTSEVIGEKIGTVGSME
                     VAETIFNALDVVSKETGVTFAFQGCEHINRAITIEKSQYNPLTMEEVSVVPNVHAGGS
                     LATYAFQHMKDPIVVEHITVPCGIDIGQTLIGMHIKHVCVPVRTSVKQVGQAIVTIAT
                     SRPKKIGGERAKYQ"
     gene            23678..24916
                     /locus_tag="NBH32_000024"
     CDS             23678..24916
                     /locus_tag="NBH32_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCD1513671.1"
                     /translation="MSYITKQDKVIAEAIEREFQRQNSNIELIASENFVSEAVMEAQG
                     SVLTNKYAEGYPGRRYYGGCEFVDVTESIAINRAKALFGAEHVNVQPHSGSQANMAVY
                     LVALEMGDTVLGMNLSHGGHLTHGAPVNFSGKFYNFVEYGVDKDTERINYDEVRKLAL
                     EHKPKLIVAGASAYSRTIDFKKFKEIADEVNAKLMVDMAHIAGLVAAGLHPNPVEYAD
                     FVTTTTHKTLRGPRGGMILCKEEYKKDIDKTIFPGIQGGPLEHVIAAKAVAFGEALEN
                     NFKTYQQQVVKNAKVLAEALINEGFRIVSGGTDNHLVAVDVKGSIGLTGKEAEETLDS
                     VGITCNKNTIPFDQEKPFVTSGIRLGTPAATTRGFDEKAFEEVAKIISLALKNSKDEE
                     KLQQAKERVAKLTAEYPLYQ"
     gene            24944..25573
                     /gene="upp"
                     /locus_tag="NBH32_000025"
     CDS             24944..25573
                     /gene="upp"
                     /locus_tag="NBH32_000025"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCD1513672.1"
                     /translation="MSKVHVFDHPLIQHKLSYIRDVNTGTKEFRELVDEVGMLMAYEV
                     TRDLELQDVDIETPVTKMTAKRLAGKKLAIVPILRAGLGMTDGILSLVPAARVGHIGL
                     YRDPETLKAVEYFAKLPQDITERQIIVVDPMLATGASAIEAITSLKKRGAKNIRFMCL
                     IAAPEGVEKMHEAHPDVDIYIAALDEKLNDKAYITPGLGDAGDRLFGTK"
     gene            25597..26724
                     /gene="wecB"
                     /locus_tag="NBH32_000026"
     CDS             25597..26724
                     /gene="wecB"
                     /locus_tag="NBH32_000026"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCD1513673.1"
                     /translation="MKKIMTIFGTRPEAIKMAPLVKALEQEKMLEPIVVVTAQHREML
                     DSVLSTFEIKPKYDLNIMKSGQTLSEITSKSITQLEQVIQLEKPDMVLVHGDTMTTFA
                     GGLAAFYNQVPIGHVEAGLRSYDKYSPFPEEVNRQLVGVLADLHFAPTKNAASHLLSE
                     GKYSESVVVTGNTAIDAMKYTVDDNYKSNIMDKYHDKKFILMTAHRRENIGQPMENIF
                     KAVRRLIDEYTDLALVYPMHKNPKVREVAQKILGSHDRIELIEPLDVVDFHNFAKKSY
                     FILTDSGGIQEEAPSFNKPVLVLRSVTERPEGVEAGTLKVIGTNKQNVYQAAKELIDD
                     ERLYHQMSEASNPYGDGFASERIVNHIKYYLNLITEKPSDF"
     gene            26792..27244
                     /locus_tag="NBH32_000027"
     CDS             26792..27244
                     /locus_tag="NBH32_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase subunit I"
                     /protein_id="HCD1513674.1"
                     /translation="MNRIDTTNRLYYHLVCMSGTVFMIDISHTEAYKMSRFYNIFKQF
                     IQYYLYLLIILGGLYLFTHHAFILGLIIGVTGSAINTVIFESYLAKAKRPDTMHISTG
                     NMWRYLVAIIACMIWYFNKSHVSIIGIIIGLMISYVVVIIRPLLKVSK"
     gene            27265..27993
                     /gene="atpB"
                     /locus_tag="NBH32_000028"
     CDS             27265..27993
                     /gene="atpB"
                     /locus_tag="NBH32_000028"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCD1513675.1"
                     /translation="MDHKSPLVSWNLFGFDIVFNLSSILMILVTAFLVFLLAIICTRN
                     LKKRPTGKQNFVEWIFDFVRGIIEGNMAWKKGGQFHFLAVTLILYIFIANMLGLPFSI
                     VTKDHTLWWKSPTADATVTLTLSTTIILLTHFYGIKMRGTKQYLKGYVQPFWPLAIIN
                     VFEEFTSTLTLGLRLYGNIFAGEILLTLLAGLFFNEPAWGWIISIPGLIVWQAFSIFV
                     GTIQAYIFIMLSMVYMSHKVADEH"
     gene            28037..28249
                     /gene="atpE"
                     /locus_tag="NBH32_000029"
     CDS             28037..28249
                     /gene="atpE"
                     /locus_tag="NBH32_000029"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCD1513676.1"
                     /translation="MNLIAAAIAIGLSALGAGIGNGLIVSRTVEGVARQPEARGQLMG
                     IMFIGVGLVEALPIIGVVIAFMTFAG"
     gene            28447..28968
                     /locus_tag="NBH32_000030"
     CDS             28447..28968
                     /locus_tag="NBH32_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCD1513677.1"
                     /translation="MTETANLFVLGAAGGVEWGTVIVQVLTFIVLLALLKKFAWGPLK
                     DVMDKRERDINRDIDDAEQAKLNAQKLEEENKQKLKETQEEVQKILEDAKVQARQQQE
                     QIIHEANVRANGMIETAQSEINSQKERAIADINNQVSELSVLIASKVLRKEISEQDQK
                     ALVDKYLKEAGDK"
     gene            28968..29507
                     /locus_tag="NBH32_000031"
     CDS             28968..29507
                     /locus_tag="NBH32_000031"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCD1513678.1"
                     /translation="MVKVANKYAKALFDVSLDTNNLETINQELTVINEAVKDKIEQLK
                     MVDSNPTQTAEQRRELINGVFTDINPYIKNMMYVLADNRHISLIADVFKAFQSLYNGH
                     YNQDFATIESTYELSQEELDKIVKLVTQQTKLSKVIVDTKINPDLIGGFRVKVGTTVL
                     DGSVRNDLVQLQRKFRRVN"
     gene            29529..31037
                     /locus_tag="NBH32_000032"
     CDS             29529..31037
                     /locus_tag="NBH32_000032"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCD1513679.1"
                     /translation="MAIKAEEISALLRSQIENYESEMSVTDVGTVLQIGDGIALIHGL
                     NDVMAGELVEFHNGVLGLAQNLEESNVGVVILGPYTGITEGDEVKRTGRIMEVPVGEE
                     LIGRVVNPLGQPIDGQGPINTTKTRPVEKKATGVMDRKSVDEPLQTGIKAIDALVPIG
                     RGQRELIIGDRQTGKTTIAIDTILNQKDQGTICIYVAIGQKDSTVRANVEKLRQAGAL
                     DYTIVVAASASEPSPLLYIAPYSGVTMGEEFMFNGKHVLIVYDDLTKQAAAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLNDDLGGGSITALPIIETQAGDISAYVPTNV
                     ISITDGQIFLQSDLFFSGVRPAINAGQSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     ESFAQFGSDLDEFTASKLERGKRTVEVLKQDQNKPLPVEHQVLIIYALTKGYLDDIPV
                     VDITRFEDELNHWAESNATELLNEIRETGGLPDAEKFDTAINEFKKSFSKSE"
     gene            31068..31934
                     /locus_tag="NBH32_000033"
     CDS             31068..31934
                     /locus_tag="NBH32_000033"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500821.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCD1513680.1"
                     /translation="MASLKEIDTRIKSTKKMKQITKAMNMVSSSKLRRAEKNTKQFTP
                     YMDKMQDAITAVAGASSNTNHPMLRPRKITRSGYLVITSDKGLAGAYSANVLKKLITD
                     IEAKHQDSSEYSIVVLGQQGVDFLKNRGYDIEYSQVDVPDQPSFKSVQALANHAIDLY
                     SEEEIDELNIYYSHYVSVLENKPTSRQVLPLSQEDSSKGHGHLSSYEFEPDKESILSV
                     ILPQYVESLIYGTILDAKASEHATRMTAMKNATDNATELIDDLSLEYNRARQAEITQQ
                     ITEIVGGSAALE"
     gene            31956..33368
                     /gene="atpD"
                     /locus_tag="NBH32_000034"
     CDS             31956..33368
                     /gene="atpD"
                     /locus_tag="NBH32_000034"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017636767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCD1513681.1"
                     /translation="MGIGRVTQVMGPVIDVRFEHNEVPKINNALVIDVPKEEGTIQLT
                     LEVALQLGDDVVRTIAMDSTDGVQRGMDVKDTGKEISVPVGDETLGRVFNVLGETIDL
                     KEEISDSVRRDPIHRQAPAFDELSTEVQILETGIKVVDLLAPYIKGGKIGLFGGAGVG
                     KTVLIQELINNIAQEHGGISVFAGVGERTREGNDLYFEMSDSGVIKKTAMVFGQMNEP
                     PGARMRVALSGLTMAEYFRDEQGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTTKGSVTSIQAVFVPADDYTDPAPATAFAHLDATTNLERKLTE
                     MGIYPAVDPLASTSRALEPSIVGQEHYEVARDVQSTLQKYRELQDIIAILGMDELSDE
                     DKQTVERARRIQFFLSQNFHVAEQFTGQKGSYVPVKTTVANFKDILDGKYDHIPEDAF
                     RLVGSMDDVIAKAKDMGVEV"
     gene            33388..33792
                     /locus_tag="NBH32_000035"
     CDS             33388..33792
                     /locus_tag="NBH32_000035"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCD1513682.1"
                     /translation="MNTLNLDIVTPNGSVYNRDNVELVVMQTTAGEIGVMSGHIPTVA
                     ALKTGFVKVKFHDGTEYIAVSDGFVEVRKDKVSIIVQTAETAREIDVERAKLAKARAE
                     SHLENDDDNTDIHRAERALERANNRLRVAELK"
     gene            34244..34336
                     /locus_tag="NBH32_000036"
     CDS             34244..34336
                     /locus_tag="NBH32_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513683.1"
                     /translation="MLKSKFPSPLSTVDISFFESSYIFSCQDTL"
     gene            34468..34701
                     /locus_tag="NBH32_000037"
     CDS             34468..34701
                     /locus_tag="NBH32_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HCD1513684.1"
                     /translation="MDYIGQYAVIHLVLHVVCICIAYWALQSIRLDQFFKKGYATQLQ
                     VCMIFVAILLGTAVSNFIVDLLQYSTQVKYLIK"
     gene            34812..36077
                     /gene="murA"
                     /locus_tag="NBH32_000038"
     CDS             34812..36077
                     /gene="murA"
                     /locus_tag="NBH32_000038"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCD1513685.1"
                     /translation="MDKIVIKGGNKLTGEVKVEGAKNAVLPILTASLLASDKPSKLVN
                     VPALSDVETINNVLTTLNADVTYKKDENAVVVDATKTLNEEAPYEYVSKMRASILVMG
                     PLLARLGHAIVALPGGCAIGSRPIEQHIKGFEALGAEIHLENGNIYANAKDGLKGTSI
                     HLDFPSVGATQNIIMAASLAKGKTLIENAAKEPEIVDLANYINEMGGRITGAGTDTIT
                     INGVESLHGVEHAIIPDRIEAGTLLIAGAITRGDIFVRGAIKEHMASLVYKLEEMGVE
                     LDYQEDGIRVRAEGELQPVDIKTLPHPGFPTDMQSQMMALLLTANGHKVVTETVFENR
                     FMHVAEFKRMNANINVEGRSAKLEGKSQLQGAQVKATDLRAAAALILAGLVADGKTSV
                     TELTHLDRGYVDLHGKLKQLGADIERIND"
     gene            36111..36551
                     /gene="fabZ"
                     /locus_tag="NBH32_000039"
     CDS             36111..36551
                     /gene="fabZ"
                     /locus_tag="NBH32_000039"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCD1513686.1"
                     /translation="METIFDYNQIKQIIPHRQPFLLIDKVVEYEEGQRCVAIKQVSGN
                     EPFFQGHFPEYAVMPGVLITEALAQTGAVAILNSEENKGKIALFAGIDKCRFKRQVVP
                     GDTLTLEVEITKIKGPIGKGNAKATVDGQLACSCELTFAIQDVK"
     gene            complement(36609..37049)
                     /locus_tag="NBH32_000040"
     CDS             complement(36609..37049)
                     /locus_tag="NBH32_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000846746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwpF-like family protein"
                     /protein_id="HCD1513687.1"
                     /translation="MKTFKAVRFQIVNEHGRIIEYELEDGVIINKEESGTGWLLEIVI
                     SNEHYETFKEYQDSEQLLDIRVVITRPANDPALFESTVKSIKNFKTTMSIVFECHIYT
                     LRQQYAESLLEQLIDDGLSGEELKKSFNRMMQSKPKLKDEKLEE"
     gene            37243..37638
                     /locus_tag="NBH32_000041"
     CDS             37243..37638
                     /locus_tag="NBH32_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1513688.1"
                     /translation="MLNKIVIVGRLTKDAQIFEKEDRKIATFCVATHRNYKDENGEIV
                     CDYLFCKAFGKLASNIEKYTNQGTLVGITGQMRSRKYDKDGQTHFVTELYVETIKFMS
                     PKSQNNEILSDSILDIDSQNIDNHDLLEI"
     gene            38027..38722
                     /gene="sceD"
                     /locus_tag="NBH32_000042"
     CDS             38027..38722
                     /gene="sceD"
                     /locus_tag="NBH32_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase SceD"
                     /protein_id="HCD1513689.1"
                     /translation="MKKTLLASSLAVGLGIVAGNAGHEAHASEADLNKASLAQMAQSN
                     DQTLNQKPIEAGAYNYTFDYEGFTYHFESDGTHFAWNYHATGANGADMSAQAPATNNV
                     APSAVQANQVQSQEVEAPQNAQTQQPQASTSNNSQVTATPTESKASEGSSVNVNDHLK
                     QIAQRESGGNIHAVNPTSGAAGKYQFLQSTWDSVAPAKYKGVSPANAPESVQDAAAVK
                     LYNTGGAGHWVTA"
     regulatory      38927..39029
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            39109..39798
                     /gene="tenA"
                     /locus_tag="NBH32_000043"
     CDS             39109..39798
                     /gene="tenA"
                     /locus_tag="NBH32_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiaminase II"
                     /protein_id="HCD1513690.1"
                     /translation="MEFSQKLYQAAKPIINDIYEDDFIQKMLLGNIQADALRHYLQAD
                     AAYLKEFTNLYALLIPKMNSMNDVKFLVEQIEFMVEGEVLAHDILAQIVGESYEEIIK
                     TKVWPPSGDHYRKHMYFQAHSRENAIYTIAAMAPCPYIYAELAKRSQSDHKLNREKDT
                     AKWFDFYSTEMDDIINVFEALMNKLAESMSDKELEQVKQVFLESCIHERRFFNMAMTL
                     EQWEFGGKVND"
     gene            39791..40621
                     /gene="thiD"
                     /locus_tag="NBH32_000044"
     CDS             39791..40621
                     /gene="thiD"
                     /locus_tag="NBH32_000044"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1513691.1"
                     /translation="MIKPKIALTIAGTDPTGGAGVMADLKSFHSCGVYGMGVVTSIVA
                     QNTLGVQHIHNLNHQWVDEQLDSVFNDTLPHAIKTGMIATADTMETIRHYLMQHESIP
                     YVIDPVMLAKSGDSLMDNDTKQNLQHTLLPLADVVTPNLPEAEEITGLTIDSEEKIMQ
                     AGRIFINEIGSKGVIIKGGHSNDTDIAKDYLFTNEGVQTFENERFKTKHTHGTGCTFS
                     AVITAELAKGRPLFEAVHKAKKFISMSIQYTPEIGRGRGPVNHFAYLKKEGLDDELSK
                     "
     gene            40605..41396
                     /gene="thiM"
                     /locus_tag="NBH32_000045"
     CDS             40605..41396
                     /gene="thiM"
                     /locus_tag="NBH32_000045"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCD1513692.1"
                     /translation="MNYLNKIRIENPLTICYTNDVVKNFTANGLLSIGASPAMSEAPE
                     EAEEFYKVAQALLINIGTLTAENEQDIIAIAQTANEAGLPIVFDPVAVGASTYRKQFC
                     KLLLKSAKVSVIKGNASEILALIDDTATMKGTDSDANLDAVTIAKKAYATYKTAIVIT
                     GKEDVIVQDNKAIVLANGSPLLARVTGAGCLLGGVIAGFLFRETEPDIEALIEAVSVF
                     NIAAEVAAENENCGGPGTFSPLLLDTLYHLNETTYQQRIRIQEVE"
     gene            41398..42039
                     /locus_tag="NBH32_000046"
     CDS             41398..42039
                     /locus_tag="NBH32_000046"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCD1513693.1"
                     /translation="MFNQSYLNVYFICGTSDVPSHRTIHEVLEAALKAGITLFQFREK
                     GESALKGNDKLVLAKELQHLCHQYDVPFIVNDDVSLAKEINADGIHVGQDDAKVKEIA
                     QYFTDKIIGLSISDLDEYAKSDLTHVDYIGVGPIYPTPSKHDAHIPVGPEMIATFKEM
                     NPQLPIVAIGGINTNNVAPIVEAGANGISVISAISKSENIEKTVNRFKDFFNN"
     gene            42125..42997
                     /gene="yidC"
                     /locus_tag="NBH32_000047"
     CDS             42125..42997
                     /gene="yidC"
                     /locus_tag="NBH32_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCD1513694.1"
                     /translation="MKKKALLPLFLGIMVFLAGCDYSKPEKRSGFFYNTFVDPMKNVL
                     DWLGNNLLNDNYGLAIIILVLVIRIILLPFMLSNYKNSHMMRQKMKVAKPEVEKIQEK
                     VKRARTQEEKMAANQELMQVYKKYDMNPIKSMLGCLPMLIQLPIIMGLYFVLKDQLVD
                     GLFKYPHFLWFDLGRPDIWITIIAGVLYFIQAYVSSKTMPDEQRQMGYMMMVISPIMI
                     IWISLSSASALGLYWSVSAAFLVVQTHFANIYYEKVAKKEVQPFIEAYEREHNGGSNK
                     KGKNTQVVSKKKKK"
     gene            complement(43475..44122)
                     /locus_tag="NBH32_000048"
     CDS             complement(43475..44122)
                     /locus_tag="NBH32_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1513695.1"
                     /translation="MQQSDIISAAKKYMESIHQNDYTGHDIAHVYRVTALAKSIAENE
                     GVNDTLVIELACLLHDTVDEKVVDANKQYVELKSFLSSLSLSTEDQEHILFIINNMSY
                     RNGKNDHVTLSLEGQIVRDADRLDAIGAIGVARTFQFAGHFGEPMWTEHMSLDKINDD
                     LVEQLPPSAIKHFFEKLLKLESLMHTDTAKMIAKERQDFMMMYLKQFFTEWNYHD"
     gene            complement(44150..45634)
                     /gene="cls"
                     /locus_tag="NBH32_000049"
     CDS             complement(44150..45634)
                     /gene="cls"
                     /locus_tag="NBH32_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1513696.1"
                     /translation="MIELLSIALKHSNIILNSIFIGAFILNLLFAFTIIFMERRSANS
                     IWAWLLVLVFLPLFGFILYLLLGRQIQRDQIFKIDKEDKKGLELIVDEQLAALKNENF
                     SNSNYQIVKFKEMIQMLLYNNAAFLTTDNDLKIYTDGQEKFDDLIQDIRNATDYIHFQ
                     YYIIQNDELGRTILNELGKKAEQGVEVKILYDDMGSRGLRKKGLRPFRNKGGHAEAFF
                     PSKLPLINLRMNNRNHRKIVVIDGQIGYVGGFNVGDEYLGKSKKFGYWRDTHLRIVGD
                     AVNALQLRFILDWNSQATRDHISYDDRYFPDVNSGGTIGVQIASSGPDEEWEQIKYGY
                     LKMISSAKKSIYIQSPYFIPDQAFLDSIKIAALGGVDVNIMIPNKPDHPFVFWATLKN
                     AASLLDAGVKVFHYDNGFLHSKTLVIDDEIASVGTANMDHRSFTLNFEVNAFIYDQQI
                     AKKLKQAFIDDLAVSSELTKERYAKRSLWIKFKEGISQLLSPIL"
     gene            45800..46093
                     /gene="csoR"
                     /locus_tag="NBH32_000050"
     CDS             45800..46093
                     /gene="csoR"
                     /locus_tag="NBH32_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-sensing transcriptional repressor CsoR"
                     /protein_id="HCD1513697.1"
                     /translation="MTEQDIAHHSEQIKTNLKSRLNRIEGQVRAINRMIEEDVYCDDV
                     LTQIRATRSALNSVATKLLEQHMKSCIMNKVNQGAQEEAMEELLVTFQKLIKD"
     gene            46105..46314
                     /locus_tag="NBH32_000051"
     CDS             46105..46314
                     /locus_tag="NBH32_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HCD1513698.1"
                     /translation="MIHQNTIYTAGIETEEQVSQLTERISNMIGVHQVNINIIDGQVT
                     VSYETPANLNSIEKEIYDEGYKIVF"
     gene            46442..46579
                     /locus_tag="NBH32_000052"
     CDS             46442..46579
                     /locus_tag="NBH32_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513699.1"
                     /translation="MKRPEKIQNVVKLLSSLGVNIKKTKSRLDIINTLPASNKVSHEL
                     K"
     gene            complement(46843..48045)
                     /locus_tag="NBH32_000053"
     CDS             complement(46843..48045)
                     /locus_tag="NBH32_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HCD1513700.1"
                     /translation="MNYSSRQQPDKHWLRKVDWVLVATIAVLAIFSVLLINSAMGGGQ
                     YSANFGIRQIFYYILGAIFAGIIMFISPKKIKHYTYLLYFLICLLLIGLLVIPESPIT
                     PIINGAKSWYTFGPISIQPSEFMKIILILALARVVSRHNQFTFNKSFQSDLLLFFKII
                     GVSLVPSILILLQNDLGTTLVLAAIIAGVMLVSGITWRILAPIFITGIVGAMTVILGI
                     LYAPSLIENLLGVQLYQMGRINSWLDPYTYSSGDGYHLTESLKVIGSGQLLGKGYNHG
                     EVYIPENHTDFIFSVIGEELGFIGSVILILIFLFLIFHLIRLAAKIEDQFNKIFIVGF
                     VTLLVFHILQNIGMTIQLLPITGIPLPFISYGGSALWSMMTGIGIVLSIYYHEPKRYV
                     DLYHPKSN"
     gene            48363..49433
                     /locus_tag="NBH32_000054"
     CDS             48363..49433
                     /locus_tag="NBH32_000054"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCD1513701.1"
                     /translation="MTKENICIVFGGKSAEHEVSILTAQNVLNAIDKDKYHVDIIYIT
                     NDGDWRKQNNITAEIKSTDELHLENGEALEISQLLKESSSGQPYDAVFPLLHGPNGED
                     GTIQGLFEVLDVPYVGNGVLSAASSMDKLVMKQLFEHRGLPQLPYISFLRSEYEKYEH
                     NILKLVNDKLNYPVFVKPANLGSSVGISKCNNEAELKEGIKEAFQFDRKLVIEQGVNA
                     REIEVAVLGNDYPEATWPGEVVKDVAFYDYKSKYKDGKVQLQIPADLDEDVQLTLRNM
                     ALEAFKATDCSGLVRADFFVTEDNQIYINETNAMPGFTAFSMYPKLWENMGLSYSELI
                     TKLIELAKERHQDKQKNKYKID"
     gene            49448..50806
                     /locus_tag="NBH32_000055"
     CDS             49448..50806
                     /locus_tag="NBH32_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCD1513702.1"
                     /translation="MINVTLKQIQSWIPCEIEDQFLNQEINGVTIDSRAISKNMLFIP
                     FKGENVDGHRFVSKALQDGAGAAFYQRGTPIDENVSGPIIWVEDTLTALQQLAQAYLR
                     HVNPKVIAVTGSNGKTTTKDMIESVLHTEFKVKKTQGNYNNEIGLPLTILELDNDTEI
                     SILEMGMSGFHEIEFLSNLAQPDIAVITNIGESHMQDLGSREGIAKAKSEITIGLKDN
                     GTFIYDGDEPLLKPHVKEVENVKCISIGVAADNALVCSVDDRDTTGISFTINNKEHYD
                     LPILGKHNMKNATIAIAVGHELGLTYNTIYQNLKNVSLTGMRMEQHTLENDITVINDA
                     YNASPTSMRAAIDTLSTLTGRRILILGDVLELGENSKEMHISVGNYLEEKHIDVLYTF
                     GNEAKYIYDSGQQHVEKAQHFNSKDDMIEVLTSDLKAHDRVLVKGSRGMKLEEVVNAL
                     IS"
     gene            51322..52842
                     /locus_tag="NBH32_000056"
     CDS             51322..52842
                     /locus_tag="NBH32_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1513703.1"
                     /translation="MQNFKELGISDNTVQSLESMGFKEPTPIQKDSIPYALQGIDILG
                     QAQTGTGKTGAFGIPLIEKVVGKQGVQSLILAPTRELAMQVAEQLREFSRGQGVQVVT
                     VFGGMPIERQIKALKKGPQIVVGTPGRVIDHLNRRTLKTDGIHTLILDEADEMMNMGF
                     IDDMRFIMDKIPAVQRQTMLFSATMPKAIQALVQQFMKSPKIIKTMNNEMSDPQIEEF
                     YTIVKELEKFDTFTNFLDVHQPELAIVFGRTKRRVDELTSALISKGYKAEGLHGDITQ
                     AKRLEVLKKFKNDQINILVATDVAARGLDISGVSHVYNFDIPQDTESYTHRIGRTGRA
                     GKEGIAVTFVNPIEMDYIRQIEDANGRKMSALRPPHRKEVLQAREDDIKEKVENWMSK
                     ESESRLKRISTELLNEYNDVDLVAALLQELVEANDEVEVQLTFEKPLSRKGRNGKPSG
                     SRNRNSKRGNPKFDSKSKRSKGYSSKKKSTKKFDRKEKSSGGSRPMKGRTFADHQK"
     gene            complement(53211..53906)
                     /locus_tag="NBH32_000057"
     CDS             complement(53211..53906)
                     /locus_tag="NBH32_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1513704.1"
                     /translation="MQSKILIIEDDHAITHLLDVALTLDYYNVTTADNATQAHFKIQI
                     DKPDVILLDLGLPDKDGLCLISEIRQHTDIPIIVISARQEEQTIIQALDNGANDYMTK
                     PFNVDELRARIRVIERIAKSHQETNIVFTNGLLSIDFGSKSVVINNQEVHLTPNEFSL
                     LELLSNHKGKVLTYEMILKRIYGYVNKTEMPSLRVHMTSLRQKLSQCHEDAKDIIKTH
                     PRIGYQMLQWKEK"
     gene            complement(53906..56563)
                     /locus_tag="NBH32_000058"
     CDS             complement(53906..56563)
                     /locus_tag="NBH32_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HCD1513705.1"
                     /translation="MSNTESLNIGKKRGSLTIYIGYSPGVGKTFEMLSNAIELFQSNV
                     DIKIGYIEPHQRDETNALAEQLPKIATNFTKHGSHHFQYLDVDRIIKESPTIVLIDEL
                     AHTNISRDRHEKRYMDIEEILNHGIDVHTTLNIQHIESLSSQIELMTGVHVKERVPDY
                     FIMSADVLEVVDISPEQLIKRLKAGKVYKKDRLDVAFSNFFTYTHLSELRTLTLRTVA
                     DLMSDKEKVRHNHKTSLKPHIAVAISGSIYNEAVIKEAFHIAQKEHAKFTAIYIDVFE
                     KNRQYKDSQKQVHQHLMLAKSLGAKVKVVYSQTVALGLDEWCKNQDVTKLIIGQHIRN
                     KWRDFFNTPLIDHLMSFEHSYKIEIVPIKQIPVELKMNKSPYRPKGKRFAIDMLKMIL
                     IQIICVMMGLWIYQLDKHESSTIILMIFLIGIILLSIWTRSYIIGFLAAIINVFVFNY
                     FFTEPRYTFEVYRFDYPITFIVSILTSILTSALLKQIKFQYSITKKQLYRTDLLFQFN
                     DSIKQTYTVENLLINAGYQINQLLQQSITVYVINQSKVIKTIPLQNHIDNTTQQHEQA
                     LSWVIKNERQAGATTDTFPGINKWLIPIGTSPIKGILAIDYQSSQVINPYDASILESM
                     LNELSLAVENVSLLKQTRESMLQAERQLTHSNFLRSISHDIRTPLTTIMGNLDILVSH
                     SKDMSIIEKEQLLVHSFQESQYLYLLVTNILSLTKLQSSNVQIKLQPYLVSELVEEID
                     MILERRHLKKRITVSSSVDLQFIHIDSKLILQALFNLIENAVKHTSTDTKINLSIRYA
                     SDEQIEFAVIDEGPGISLEEQQKIFEPFYTGSNKYFKDNQKESMGLGLYLVQTILHKH
                     QSNLQYKPNQPHGSIFYFNIDTDFNEGDI"
     gene            56701..56811
                     /gene="kdpF"
                     /locus_tag="NBH32_000059"
     CDS             56701..56811
                     /gene="kdpF"
                     /locus_tag="NBH32_000059"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCD1513706.1"
                     /translation="MLLCCKEVMAMLIVLLLVVIALVLYLFYALIRSEKF"
     gene            56835..58511
                     /gene="kdpA"
                     /locus_tag="NBH32_000060"
     CDS             56835..58511
                     /gene="kdpA"
                     /locus_tag="NBH32_000060"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit A"
                     /protein_id="HCD1513707.1"
                     /translation="MEIILFLTMMVMITYVFSGYLYRVALVQSSRVDLIFTRFENMCF
                     KIIGTDLEHMSAKTYVKHFLAFNGFMGGISFVLLIVQQWLFLNPNHNLNQSIDLAFNT
                     AISFLTNSNLQHYNGESGVTYLTQMIVMTYLMFTSSASGYAVCITMLRRLTGLTNIIG
                     NFYQDIVRFIVRVLLPLSCLISILLMTQGVPQTLHANLMIRTLSGHIQHIAFGPIASL
                     ESIKHLGTNGGGFLAGNSATPFENPNIWSNFIEMGSMMLLPMSMLFLFGRMLSRHGKR
                     VHRHALILFVAMFFIFIAILTLTMWSEYRGNPILANLGIYGPNMEGKEVRFGAGLSAL
                     FTVITTAFTTGSVNNMHDSLTPLGGLGPMVLMMLNVVFGGEGVGLMNLLIYVLLTVFI
                     CSLMVGKTPEYLNMPIGAREMKCIVLVFLIHPILSLVFSALAFMIPGASESITNPSFH
                     GISQVMYEMTSAAANNGSGFEGLKDDTTFWNISTGIIMLLSRYIPIILQLMIASSLVN
                     KKSYHQDKYTIAIDKPYFGVSLIVFIVLLSGLTFIPVLLLGPIGEFLTLK"
     gene            58530..60557
                     /gene="kdpB"
                     /locus_tag="NBH32_000061"
     CDS             58530..60557
                     /gene="kdpB"
                     /locus_tag="NBH32_000061"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000546595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HCD1513708.1"
                     /translation="MHHVNKYFNQTMVIEALKMSFYKLNPKQLIKNPIMFVVEVGMIL
                     TLILICFPDIFGTSYLSRGYLITIFIILLITILFANFSEAFAEGRGKAQADSLRQAQS
                     NLTARLIEENGAYRIVNATELKAGQNIRVENGETIPADGVVINGLATVDESAITGESA
                     PVIKESGGDFDGVIGGTLVTSDWLEIRVESEAGTSFLDKMIALVEGAERNKTPNEIAL
                     FTLLTTLTIIFLVVIETLYPIASYLHLILPIAMLIALTVCLIPTTIGGLLSAIGIAGM
                     DRVTQFNVLAKSGRAVEVCGDVDVMILDKTGTITYGNRIASEFLPVNQQMLEKLIVAA
                     YMSSIYDDTPEGKSIVRLAKQMYINELPKDIDGTYKPFTAETRMSGIITNEISVFKGA
                     PNSMINLVKQQQGNIPLNIESLCMDVSSKGGTPLIVIENNVMLGVIYLKDVIKDGLVE
                     RFTELRKMGIETVMCTGDNALTAATIAKEAGVDRFVAECKPEDKIKVIKDEQAKGHIV
                     AMTGDGTNDAPALAQANIGLAMNSGTISAKEAANLIDLDSNPTKLIEVVKIGKQLLMT
                     RGALTTFSLANDVAKYFAILPALMMSTIPEMTSLNIMHLSSPKSAIISALIFNALIIV
                     ALIPIAMKGVKVKGYSIDRIFINNMLIYGLGGLIVPFLGIKLIDMIVQFFV"
     gene            60577..61137
                     /locus_tag="NBH32_000062"
     CDS             60577..61137
                     /locus_tag="NBH32_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCD1513709.1"
                     /translation="MNTIRNSICLTIITIVLCGFLFPLAITLIGQIFFYQQANGSLIT
                     YDNRIVGSKLIGQHWTERRYFYGRPSAVDYNMNPEKLYKNGVSSGGSNESNGNNELIA
                     RVKHHVKFGNSNVTIDAATSSGSGLDPHITVENALKQAPRIADVRHISTSRVADLIQH
                     RKQRGVLTNDYVNVLELNIALDNMKD"
     gene            61346..61825
                     /locus_tag="NBH32_000063"
     CDS             61346..61825
                     /locus_tag="NBH32_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513710.1"
                     /translation="MSDYTKLSKSPKSALLYYYITNGLEFILSVAVYVIFYFIWLRFE
                     WPHYLLYILLVLGALSTLKLFIKPLWQYHCRFYQVDQLSVQYRTSFLIYKEETSRIER
                     LQYLSIKSNPISKVLNLYKVGFMTAGHTINLPMMSHDDVKIIEARAMSNLRGVESDV"
     gene            61818..63401
                     /locus_tag="NBH32_000064"
     CDS             61818..63401
                     /locus_tag="NBH32_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1513711.1"
                     /translation="MSEPQKLHPISYFNGIINAIKQNIVVFFIFIIFQLKDFDYTNPE
                     SYLWIGIVFVFFLISYISQIVGIMNTRYWIEDNYFILTTGIFNKKRKELNIKRIQSVD
                     MTQGVVNQIIGGVDLQIKTPSDGIVLSVISKKQGEYLERYIDQLQTELKTEGAQKSSA
                     EKNDNCNHDIEGNVEDIAEGRLDEVQNTDSMLNQKNKRIPIYQMNFKESLFMAMTSGA
                     IGVTLAALVPIYGAVRELIPWSKLNHEISQWVQIVSISVLIIILLVLIVAYIIGTIIT
                     MIRFYGFSVMLENEQLKIEYGLFNKKKVTVPTRRLQAVVERQSYIRRIFGYTAIHFII
                     TSDFENKKIEDDDNDSGSVTILPFIKRDKAYQIIQNLVPDLSYQQVNEGMPFSGFHRH
                     FLMPSIIVIAMAGIGCYFWNQLLLLFTIITIVIIGLFVIKGYLYVKHAGFKMTEEQIT
                     IQNFELLKRNIYYFKAHHIIGFDTWQHTLLKRNNLNNFSFIVAKGVGAQSINLKYARS
                     EDANKLKSWYLRGENDEGL"
     gene            63388..63879
                     /locus_tag="NBH32_000065"
     CDS             63388..63879
                     /locus_tag="NBH32_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1513712.1"
                     /translation="MRDYNFMSPHAKKVMRLSAVMFWIPIALVLLIAFNILNWLFWEI
                     LDNYISIVSSVIIILLVAVFTLVIAPVYRYKHCRYAFEKHHLRVRNGILFLDEKVIPY
                     FRIQNIDIDVGPIMRRYRLATLTLFTAGGQAEIELIDRSEARRLKRWLNNERHLENNK
                     NEE"
     gene            63883..64242
                     /locus_tag="NBH32_000066"
     CDS             63883..64242
                     /locus_tag="NBH32_000066"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCD1513713.1"
                     /translation="MIHGIGVDLIEIDRIKALYSKQPKLVERILTKNEQHKFNNFTHE
                     QRKIEFLAGRFATKEAFSKALGTGLGKHVAFNDIDCYNDELGKPKIDYEGFIVHVSIS
                     HTEHYAMSQVVLEKSAF"
     gene            64308..65456
                     /gene="alr"
                     /locus_tag="NBH32_000067"
     CDS             64308..65456
                     /gene="alr"
                     /locus_tag="NBH32_000067"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1513714.1"
                     /translation="MSDKYYRSAYMNVDLNAVASNFKVFSTLHPNKTVMAVVKANAYG
                     LGSVKVARHLMENGATFFAVATLDEAIELRMHGITAKILVLGVLPAKDIDKAIQHRVA
                     LTVPSKQWLKEAIKNISGEQEKKLWLHIKLDTGMGRLGIKDTKTYQEVIEIIQQYEQL
                     VFEGVFTHFACADEPGDMTTEQYQRFKDMVNEAIKPEYIHCQNSAGSLLMDCQFCNAI
                     RPGISLYGYYPSEYVQQKVKVHLKPSVQLIANVVQTKTLQAGESVSYGATYTATDPTT
                     IALLPIGYADGYLRIMQGSFVNVNGHQCEIIGRVCMDQTIVKVPNQVKAGDSVILIDN
                     HRESPQSVEVAAEKQHTINYEVLCNLSRRLPRIYHDGDQRFVTNELLK"
     gene            65541..65711
                     /gene="mazE"
                     /locus_tag="NBH32_000068"
     CDS             65541..65711
                     /gene="mazE"
                     /locus_tag="NBH32_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin MazE"
                     /protein_id="HCD1513715.1"
                     /translation="MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDC
                     NESYLSSNSTNE"
     gene            65708..66070
                     /locus_tag="NBH32_000069"
     CDS             65708..66070
                     /locus_tag="NBH32_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCD1513716.1"
                     /translation="MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVA
                     AITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIRTLDKKRLKEKLTYLSDDKMKEV
                     DNALMISLGLNAVAHQKN"
     gene            66419..67420
                     /locus_tag="NBH32_000070"
     CDS             66419..67420
                     /locus_tag="NBH32_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HCD1513717.1"
                     /translation="MEEFKQHYKGLIDESLTCQDKVELIKKCEKYTDEVIRKDVLPED
                     IVDIHKNYILTLNLTREDVFKTLDVLQEIVKGFGYSYRDYQRLVDKLQVHDKEIDLAS
                     SLQQTMLKTDIPQFDSIQIGVISVAAQKVSGDYFNLIDHNDGTMSFAVADVIGKGIPA
                     ALAMSMIKFGMDSYGHSQLPSDGLKRLNRVVEKNINQNMFVTMFYGLYEEMNHLLYCS
                     SAGHEPGYIYRAEKEEFEEISVRGRVLGISSQTRYQQQEIPIYLDDLIIILTDGVTEA
                     RNSEGTFIDKQKLLEYIKKHKHMHPQDIVQIIYEAILKLQNPNKKDDMTILIIKRVN"
     gene            67540..67866
                     /locus_tag="NBH32_000071"
     CDS             67540..67866
                     /locus_tag="NBH32_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor antagonist"
                     /protein_id="HCD1513718.1"
                     /translation="MNLNIETTTQDKFYEVKVGGELDVYTVPELEEVLTPMRQDGTRD
                     IYVNLENVSYMDSTGLGLFVGTLKALNQNDKELYILGVSDRIGRLFEITGLKDLMHVN
                     EGTEVE"
     gene            67868..68347
                     /gene="rsbW"
                     /locus_tag="NBH32_000072"
     CDS             67868..68347
                     /gene="rsbW"
                     /locus_tag="NBH32_000072"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001190828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma B factor RsbW"
                     /protein_id="HCD1513719.1"
                     /translation="MQSKEDFIEMRVPASAEYVSLIRLTLSGVFSRAGATYDDIEDAK
                     IAVSEAVTNAVKHAYKEKNNVGIINIYFEILEDKIKIVISDKGDSFDYETTKSKIGPY
                     DKDENIDFLREGGLGLFLIESLMDEVTVYKESGVTISMTKYIKKEQVRNNGERVEIS"
     gene            68322..69092
                     /gene="sigB"
                     /locus_tag="NBH32_000073"
     CDS             68322..69092
                     /gene="sigB"
                     /locus_tag="NBH32_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigB"
                     /protein_id="HCD1513720.1"
                     /translation="MAKESKSANEVSPEQINQWIKEHQENKNTDAQDKLVKHYQKLIE
                     SLAYKYSKGQSHHEDLVQVGMVGLIGAINRFDMSFERKFEAFLVPTVIGEIKRYLRDK
                     TWSVHVPRRIKEIGPRIKKVSDELTAELERSPSISEIADRLEVSEEEVLEAMEMGQSY
                     NALSVDHSIEADKDGSTVTLLDIMGQQDDHYDLTEKRMILEKILPILSDREREIIQCT
                     FIEGLSQKETGERIGLSQMHVSRLQRTAIKKLQEAAHK"
     gene            69527..71677
                     /locus_tag="NBH32_000074"
                     /pseudo
     CDS             69527..71677
                     /locus_tag="NBH32_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500775.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
     gene            71670..72125
                     /locus_tag="NBH32_000075"
     CDS             71670..72125
                     /locus_tag="NBH32_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HCD1513721.1"
                     /translation="MNNDKLQRMVENLSEEKFGRTFQHRAYFNKRLRTTGGRYLLKSH
                     DIEINPKQYEHYGEDAVVKIILHELCHYHLHIAGKGYQHKDRDFKRLSQQVGAPRFCN
                     SIESYQQRANYEYYCTKCHAKYIRIRKVDTTRMRCGHCNGKLRMKKQLK"
     gene            72772..72854
                     /locus_tag="NBH32_000076"
     tRNA            72772..72854
                     /locus_tag="NBH32_000076"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72805..72807,aa:Leu,seq:gag)
     gene            72876..72949
                     /locus_tag="NBH32_000077"
     tRNA            72876..72949
                     /locus_tag="NBH32_000077"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:72908..72910,aa:Gly,seq:tcc)
BASE COUNT        25853 a        10465 c        13893 g        22792 t
ORIGIN      
        1 gttcaaatta tgcaaggttt agaagcagat tcagattact aattttataa aaaacatgta
       61 agcatgggac atcgtaacca tatataagta tgtcgatatc agctttaaga catcataaat
      121 gccatgatga ttctaagata atttaatagg gactttgacg ttataaccaa tctatttaag
      181 aataataaca tcgcatttaa attgtgatat taaaaatgaa atcgatagct tgctataata
      241 attttcgctt gtgcttttaa aaatggacta gagaaagcgc agatgctttg tcctaagctt
      301 tttaagaaaa ggatgataaa aatgagaatg atagacatta ttgagaaaaa gcgtgacggt
      361 catacgctaa caacagaaga aattaatttc tttattgacg gctatgttaa aggggatatt
      421 cctgattacc aagcatcaag tttagcaatg gcgatttatt tccaagatat gaatgatgat
      481 gagcgtgcag cattaacgat ggctatggtt aattctggtg atatgataga tttgagtgat
      541 atcaaaggtg tcaaagtaga taagcactca acaggtggtg taggagatac aactacgttg
      601 gttttagcac cattagtagc agctgtagat gttcctgttg caaaaatgag tgggcgtgga
      661 ttaggtcata caggtggtac gattgataaa ttagaagcaa ttgatggttt tcatgttgaa
      721 atagatgaag caacatttgt gaaattggtt aatgaaaata aagtggcagt tgtaggacaa
      781 tcaggaaatt taactcctgc agacaaaaaa ttatatgcct taagggatgt tactggtact
      841 gtcaattcaa taccattaat tgcctcttca attatgagta aaaagattgc tgctggtgca
      901 gatgcaattg tattagatgt aaaaactggt agcggtgcat ttatgaaaac attagaagat
      961 gctgaagcat tagcgcacgc aatggtacgt attggtaata atgtgggacg taatacgatg
     1021 gcgattattt ctgatatgaa tcagccactt ggacgtgcga ttggcaatgc gcttgagtta
     1081 caagaagcaa ttgatacgtt gaaaggacat ggtccgaaag atttaacaga acttgtacta
     1141 acattaggtt ctcaaatggt tgtacttgca aacaaagctg aaacgcttga agaagcgaga
     1201 gcattgctaa ttgaggcaat tgattcaggt gcagcattag aaaaattcaa aacatttatt
     1261 aaaaaccaag gtggcgacga aacagttatt gaccatccag agcgtttgcc acaagctcaa
     1321 tatcaaatcg aatataaagc taaaaaatca ggttatgtga ctgagttagt ctctaacgat
     1381 ataggtgtcg cttcgatgat gttaggagcg ggacgtttaa caaaagagga tgatattgat
     1441 ttagcggttg gtattgtttt aaataaaaaa attggtgata aagtagaaga aggagaatca
     1501 ttattaacga ttcacagtaa tcgccaagat gtagacgatg ttgttaaaaa attagattca
     1561 agcataacaa ttgctgatca tgttgtatcg cctacattaa ttcataaaat cattacagag
     1621 taggagttga agctgattga acatactaag atatttatta ggaataggtt ttagtgtaat
     1681 aggtgtattg cattttacac gagaacgaca atttagaaat atcataccga aatgtttgcc
     1741 acttcgaaaa acagcggttc ttgttacagg aatctttgag atattatttg gacttgcact
     1801 tttaattaaa agaccatctc aatgcttgaa aaatacaatc aatctattct tgctagctgt
     1861 attcccagct aatatctata tggcagttaa aaagttacct ttaggagata aacaattgcc
     1921 aacatggcaa ctatatttaa ggttacctat gcagttcgtg ttaatgggac taattaagaa
     1981 attatagtgg ttcttaaata aatcggacaa atgttaaaga ttttactttt tcatttgttc
     2041 gattttttga ctttgaagca taataaaaag cagttggaca atggcgattg taacatcatt
     2101 gttcgactgc ttttctgatt aacttgatcg actaagattt attttcctgt accgaaaaca
     2161 tcatgccatt cgtttcgttt gtctagaaat gcttgagcga tagttttagc accttctaat
     2221 gaatgacttg ctgcccagcc acattgtact tcattacaag caggcacttc agtagctttt
     2281 agcacatcat ttaaagttgc ttcaacaata tttaatacat catcataatt atcatgatca
     2341 ataaatgata catagaaacc agtttggcaa cccataggac ttaaatcaac aactttgtca
     2401 ctatgatttc taatattttc agccattaaa tgttctaatg aatgtagtcc gggcatatcc
     2461 atatgttctt tgtttggttg tttgaaacga atgtcgtatt tgtgaatgac atctccgttt
     2521 aatccttcca tcgttcccgc taatctaata aatggggcaa ccactttagt atgatctaaa
     2581 ttgaaacttt caacattcat ttttgtcatt ttgaatttcc tcctattaac tactcaaaac
     2641 tataaaacgc ttatgatttg ttgcattata aaacttaagt ttgttgtaag ttgtgtttct
     2701 aacaatactt aaattgtaac aatagggctt atgttttaca atttgaaact gttttataaa
     2761 tttatgaaag gcttttagtt attagaaaat tttgtaaata aagaaaaact agatgacatc
     2821 atgagataaa accgatagaa taagtaataa ttcattttga aagaacaaaa tattgtggat
     2881 gattaaaatg agtgtagggt aaaagggaga tagtaaaaaa tgagtgaaaa acaacaaatt
     2941 ctcgattata tagaaacaaa taaatatagt tatatcgaaa tcagtcatag aattcatgaa
     3001 cgtcctgaac ttggtaatga agaaatattt gcgtctcgaa ctttaataga tcgtttgaaa
     3061 gagcatgatt ttgaaattga aaccgagatt gctgggcatg caactgggtt tatagcgaca
     3121 tatgattcag gtcttgacgg gccagctata ggttttttag cagaatacga tgctttgcca
     3181 ggattgggtc atgcttgtgg tcataatatc attggaactg caagtgtgct tggtgcaatt
     3241 ggtttgaagc aagtgattga ccaaattggt ggtaaagtag tcgttcttgg atgtccagct
     3301 gaagaaggtg gggaaaatgg tagcgctaaa gcttcttatg tcaaggctgg tgtgattgat
     3361 caaatagaca ttgccttaat gattcatccg ggaaatgaaa cttataaaac gattgatact
     3421 ttggcagtgg atgttttaga tgttaaattt tacggaaaaa gtgctcatgc ctctgaaaat
     3481 gcagatgaag cgttaaatgc attagacgct atgattagtt attttaatgg tgtagcacaa
     3541 ctacgacaac atattaaaaa agatcaacgt gtgcatggtg tgattctaga tggcgggaaa
     3601 gcagctaata ttattccaga ctatacacat gctcgttttt atactagagc aatgacgcgt
     3661 aaagaattgg atatattaac agaaaaagta aatcaaatcg cacgtggagc tgcgatacag
     3721 actggttgtg attatgaatt tggtccaatt caaaacggtg tgaatgaatt cattaaaacg
     3781 ccgaaattag atgatttatt tgctaaatat gctgaagaag ttggtgaagc agttattgat
     3841 gatgattttg gttatggctc tacggataca gggaacgtaa gtcatgttgt gccaacaata
     3901 catcctcata ttaaaatagg atcacgtaat ttagtaggac atacgcatag atttagagaa
     3961 gcagctgcga gtgtacatgg tgatgaagca ttaattaaag gtgctaaaat aatggcgttg
     4021 atggggttag agttaattac aaatcaagac gtttatcaag acattattga agagcatgcg
     4081 catttgaaag ggaatgggaa gtaatgacaa ataatggtaa tgagccgaac ttaacgttaa
     4141 gtgatttata tgacaaagat gttgtctata cgtcaagacc ttcgtatata tcgaatccat
     4201 ggttgaaacc ggacgaacat caatctaatt ttttaactgg tagggagtta ttaatagcaa
     4261 atcaattacc tgttattgtt cacgaagcta gtgcgacaga taaattgcat cagctatttc
     4321 aagttattgg taaggatgta ccgaactcaa tttatacatt taacaatcag caatcttacg
     4381 aaaacttgat taaacaattg gcacataagg aaaataaaaa gatttatttt caatatattc
     4441 atgatgaaac aatcttgaat cagcaatatt atgcattaga taaaacgtta tttgttgctt
     4501 taaataataa agcaagaata cctgaatgga caaatgggaa gtttttacca aaaagaaaag
     4561 ttgtaaaaat tgagcaattc gaaaacgaga ttaagaattg ggaattcccg tttgtaatta
     4621 agccgggaga tgatcttccg acagcaggtg gctatggcgt tatgatttgt tatcatgatg
     4681 cagatttaca aaaagcgata acgcgtatta aagaggcaac tgctgaaacg aattcgctta
     4741 ttatcgaaca gaagattgaa gaaaaagcga attattgcgt tcagtttgca tactcggaat
     4801 cgctaggcat tcaatatctt ggtgcggcaa cgcaattaac ggataagtac ggattttata
     4861 acggaaatga aaatactact aatgtaccag agcacgttat agaagcaggt agacaaatta
     4921 tggaaaacgg tgtgaatcaa ggattctttg gtgtagctgg ttttgaccta ctcgtcgatg
     4981 aggatgataa cgtttatgcg attgatttaa actttagaca aaatggttca acgagcatgt
     5041 tattacttgc taatgagttg aattcaggat atcaaaagtt ttatagttat cattcaaaag
     5101 gtgataacac acatttcttc aatacgattt tgaaatatgt caaagaaggt agtttatacc
     5161 cgttatctta ttatgatggt gattggtacg gtgaagataa agttaaatca aggtttggct
     5221 gtatttggca tggagattca aaagaaacag tactggagaa tgaacgcgca tttttagctg
     5281 aacttgaaca ctattagagt tcggaacata aggcgctaca atgttatgtt gccagtagtt
     5341 gactgaatat gcgcttgtaa caagcttttt tcgattctag tcaacaataa ttaaatttat
     5401 gatatggcaa tactttgtaa tactaatatt aaatggcgac ttttatttca ctatgttata
     5461 agagttgcca ttttgttgat aaaggtatac taaaggttat cgttttgaaa tttttagtaa
     5521 ctagatatgt ttcgtgttat agaccgaatt tgtgtatacg taaaatttaa tgctattgaa
     5581 tttttaaaat gaaaaacatg acattaaatt gaattcataa tatgtctaat tgactaactt
     5641 gttggagtca tttactattt tatgtatgac atattttaaa aagtgagggt caagcatgtc
     5701 ttataaagca tatccattct ttagagatat attaataaat gaatgtattt atttcgcctc
     5761 taaaaataaa aaactagtac gcctaaatta taaaagtgaa gcgtatgtag gcgtttggac
     5821 agaagaaagt gtggccgtat catttttaac aagtcgtgat attccatttg ataaagttgt
     5881 aaaaatggac gttgatcgtt ttgctactta tgaattagat gaattgtttg atgaacaaga
     5941 ccatattatt atgaatcaaa caatggaaga agaagggcat ctactaaacg ttgtagctgt
     6001 tacacaagaa gtgatgacgg aattagataa aattagaatc aaagaatttg tccaagatgt
     6061 agcgaaatat gatgaagtat acggcttaac taaaaaaggt agtaagcagt ttattctcat
     6121 tagtgaaaat gatagcgacg aaaaaaagcc gcatattatg cctgtatgga gtattaaaaa
     6181 cagagcgtta aaagttcgag atgaagattt tgaagagtgt gatttaatta cgattgaagg
     6241 ttctgttttc ggagaatggc tagatgaact tagagatgat cataaagccg ttgcgataga
     6301 tttaaaaact ggcgtggttg gtacaattgt ttcagcgcaa aaattgtcta atgaacttac
     6361 attttaattg aattgagttg taagtaatta agtgataata aatggaacaa tacgtattca
     6421 aaacacgtag accataaaat aaaaggccat ttatatagcg tttatttaaa acaacgctca
     6481 tataaatggt ctttttctat tttcctaaat ataatgcacc aatagcacct gaaaatgcgc
     6541 cgttttcaac atagtacggt ttgcaaccgc gtaacacagt ataatcttcc acaactttgc
     6601 gtaataaagc gttattatga aatgaagaac cgatataaac gatattttca gttttaaatt
     6661 cacgtgcaac agtaatggcc attgtcgtaa caacttcgcc aacgacacca ataacggctg
     6721 ctaatttatt gctaggtgta aaatcagcat ctaaatgatg tagtacatga ccaaaattag
     6781 ctgctgttaa atcaccggga atgggtggtt cggtatcttt ataaatatgt ctaaccttta
     6841 aatcgatagt gttacgatca ccgtgttgtg ccatgtcagt taactgttta taatcagtga
     6901 tttgacttag taaataaccg agtccttgaa tcatgccacc acctgtaccg ataccgccta
     6961 cacgacgttg tgattggcca tcgaaataat gtagtgacgt accggtacca acatttgcaa
     7021 aaatataatc tgctaagtca tggccttgct cttttaacaa aatacctagt ccttgagatg
     7081 cagcatcaaa ctctacaaaa atttgtgcag gaatgttgat gttttcagca atgacacctg
     7141 cattacctcc agttaagcat aatttttcaa tttgctgttg gtttaaccat tccacaactt
     7201 gatcaatatt tttagttaat tcagttttaa aagtacgttg gttatcttgc tcttgaacga
     7261 ttttaattag tgtaccgcca gcgtcaatgc caactttcat aagattccca cctcattatt
     7321 aatgtctatc cttaaataat agtatagtaa aatgactaaa aaacaagtaa taatagtaat
     7381 tattaacaaa tttgatgcca ttgtatttca acattgtaag cgtatcgcaa ttaatgtatt
     7441 acaaacgtag acgttaagtt atatatatta ttttctagga attttgaagt tgtataggat
     7501 tgttagttag tgacgcaata ttaaaagtag ttcgtacgca gtgtatttgt aagtctctga
     7561 ttaaaatgat aagtaatgag gaatagcaca ttaattttaa aaattaaaaa atataaataa
     7621 gtaatttatt taacttagag taaataatgg tatcgtagtg aaataatagg taaaataata
     7681 tggggattca tgcttcatat ataaaaagat aggggttaaa tatatggcta aagaactttg
     7741 ttttgaaggt atcactttaa aagcatttga tgaacaatat cgttcagcaa ttaatgattt
     7801 tgacttgaat gaaagacaac aaatatattc atctttacct aaagaagtta ttgatgatgc
     7861 aattaatgat gctgatagga ttgctaacgt agcaataaac gataaaaatg aagtggtggg
     7921 cttttttgta ttacatcgtt actatcagca tgaaggttat gatacacctg aaaatgtcgt
     7981 ttatattcgt tcattatcga ttaatgaaaa atatcaaggt tttggatatg gcacgaaaat
     8041 aatgatgtca ttgccgcaat atgttcaagg tgtatttcct gattttaatc atctatatct
     8101 agtagtagat gcggaaaatg acaatgcttg gaacctatac gaacgtgctg gattcatgca
     8161 tacagcgact aaagaagaag gaccaatcgg caaagaacga ctatattact tggacttaga
     8221 ttcaaaacat gtttcgtcat taaagcttga agaagaaagt cgttcagaag tgaccaatgt
     8281 acatatcatt aatttaatga ttgatggcca aaaggttggc tttatcgcat tggagcagat
     8341 tggtgaacgc atgaacattg ctgctattga agtggataaa tcatatcgct ttaatggtat
     8401 tggttcaagt gcgctgcgac aattgccaac ttacttaaga aaaaactatg acaaccttaa
     8461 tgtgattacg atgattctgt ttggagagaa taatgatttt aaaccattat gtctaaatag
     8521 taatttcgtt gaaatcgaac aaactgatga ttatgtcgtt ttcgaaaaat atttaaatta
     8581 ctaacagtga ttgcgaaata tgatattgtc atttataatt tagttttgtt actatatata
     8641 aatgaattca gacgtataaa tttagattat atccttcgaa aggaagtatt gggcaatgaa
     8701 aattcaagat tatacaaaac aaatggttga tgaaaaatca tttattgata tggcttatac
     8761 attattgaat gataaaggtg aaacaatgaa cttatatgat atcatcgatg aatttagagc
     8821 attaggtgat tatgagtatg aggaaattga aactcgtgtt gtacaatttt acacagattt
     8881 aaatacagat ggacgttttt taaatgttgg tgaaaactta tggggattac gtgactggta
     8941 ctcagtagat gatatcgaag agaaaattgc accaacaatt caaaaatttg atattttaga
     9001 cgccgatgat gaagaagatc aaaacttaaa attattgggt gaagatgaat tggatgatga
     9061 tgacgatatt ccagctcaaa ctgatgatca agaagaatta aatgatcctg aagatgagca
     9121 agttgaagaa gaaatcaatc attcggatat agtcattgaa gaagatgaag atgaactaga
     9181 tgaagacgaa gaagattttg aagacgaaga agacttcaaa gcataatttt ttgtttgact
     9241 tttagttgaa agatgataaa attttattcg ggctccttta aataggacac gtgtataaaa
     9301 tttatacgct ccccttacaa aatttgtgag agggagcgtt tttttattta attgagtaaa
     9361 tcaagaaatg ataacgcaat aatcaaagtt gtaaatgata tacatagtga catagcagta
     9421 tggaaacggt aagtaaacag aatttaattt tgtcgaattc gacaaacaaa caacttgaat
     9481 gagcttactc aaatgttatg ttttacgtaa tttttacaat tgatgaggaa gcattccctt
     9541 taataattag gaggtcaaga catgacaaaa tttatttttg taacaggtgg cgtagtttca
     9601 tcattaggga agggtattac agcatcttct ctaggtagat tattaaaaga tagaggtctt
     9661 aatgtaacaa ttcaaaaatt cgatccatac ttaaatgttg acccaggtac aatgagtcct
     9721 tatcaacatg gtgaagtatt cgtaacggat gatggtgcag aaactgacct agacttagga
     9781 cattacgaaa gatttattga tattaattta aacaagtttt caaatgtaac agccggtaaa
     9841 gtgtattcac acgtactgaa aaaagaacgt cgtggtgatt acttaggcgg aacagttcaa
     9901 gttattccgc atattacaaa tgaaattaaa gaacgtttat tacttgcagg ggaaagtacg
     9961 aatgcagacg ttgttatcac tgaaattggc ggtacaacag gtgatattga gtcattaccg
    10021 tttattgaag cgattcgtca aattcgtagt gatttaggta gagaaaatgt tatgtatgtt
    10081 cactgtacat tactgcctta tattaaagct gctggagaaa tgaaaacgaa gccaacacaa
    10141 catagtgtta aagaattacg aggtttaggt attcaaccag acttaatcgt tgtaagaact
    10201 gaatatgaaa tgacacaaga tttaaaagat aaaattgcat tattctgtga cattaataaa
    10261 gaaagtgtta ttgaatgtcg tgatgcagac tctttatacg aaattccatt acaattaagc
    10321 caacaaaata tggatgatat cgttattaaa cgtttacaat taaacgcgaa atatgaaaca
    10381 cagcttgatg aatggaaaca gttgttagat atcgttaata atttagatgg taaaattaca
    10441 attggtttag taggtaaata tgttagctta caagatgcat atttatcagt tgttgaatca
    10501 ttgaaacatg ctggatatcc ttttgccaaa gatattgaca ttagatggat tgattcaagt
    10561 gaagtaacag atgaaaatgc agccgaatac cttgcagatg tcgacggtat tttagtacca
    10621 ggtggatttg gtttccgtgc aagtgaaggt aaaattagtg caattaagta tgctagagaa
    10681 aacaatgtac cattctttgg tatttgttta ggaatgcaac ttgcaacagt tgaattttca
    10741 agaaacgtat taggccttga aggcgcacat tcagctgaac tagacccagc aacaccatac
    10801 ccaattatag atttattacc agaacaaaaa gatatcgaag atttaggtgg tacattacgc
    10861 ttaggcttat atccatgttc aattaaagaa ggtacattgg cacaagatgt ttatggtaaa
    10921 gcggaaattg aagaaagaca tcgtcatcgt tatgaattta ataatgacta tagagaacaa
    10981 ttagaagcaa atggtatggt aatttctggt acaagtccag atggacgttt agtagaaatg
    11041 gtagagattc cgacaaatga tttctttatt gcttgtcaat tccacccaga attcttatct
    11101 agaccaaatc gtccgcaccc gatttttaaa tcatttattg aagcttcatt aaaatatcaa
    11161 caaaataaat aaatttgcta ataaaaccgg tactttcatt gttaaacatt gaaagtaccg
    11221 gtttttcgta taattttaat attatgttag tgacaaggta tgaaataaca atagtgactt
    11281 ttataattct aagtctcttg tcatttcaat catttgtgta taaatgtcat agtatacata
    11341 attcaatgcc atcgcatgtg gttggacaat cttatcgtaa tcttcagtgt agactatagg
    11401 tcttggtgta gataaatcga taaaatgtac gagatgatca gggaaatcat ctgttttagg
    11461 tttgttgctt attaagacca catcgatatc taagtcgata agtttttgaa tatctaatgc
    11521 aacttgatca ttataaaatg gtgcgaataa taatacacga tcagttgagt caatttcttt
    11581 aaagtcttta atagcgtcaa gttttcggct agattttaaa cgctcatcac catgtagaat
    11641 gaaactttcg aaaaactgta aatcatcata accttttaca taaacataac cttcgccacc
    11701 aattgcttga attaaacatt gggcagccat ttgaatttcc aaagattgtt tttctagcct
    11761 attaaagata cctattagtt gtgtgtttaa gatttttgac atctttatcc tccaatctac
    11821 ttataaaata ttgtaattaa tgactacata ttatgcaacg gcttaaattg tataaaaatg
    11881 tatacgtttg catttattat aactatcgca tttttcaaaa aatacacatt taatctgcag
    11941 tatttcaatg cattgacgct atttttttga tataattact ttgaaaaata cgtgcgtaag
    12001 cactcaagga ggaactttca tgcctttagt ttcaatgaaa gaaatgttaa ttgatgcaaa
    12061 agaaaatggt tatgcggtag gtcaatacaa tattaataac ctagaattca ctcaagcaat
    12121 tttagaagcg tcacaagaag aaaatgcacc tgtaatttta ggtgtttctg aaggtgctgc
    12181 tcgttacatg agcggtttct acacaattgt taaaatggtt gaagggttaa tgcatgactt
    12241 aaacatcact attcctgtag caatccattt agaccatggt tcaagcttcg aaaaatgtaa
    12301 agaagctatc gatgctggtt tcacatcagt aatgatcgat gcttcacaca gcccattcga
    12361 agaaaacgta gcaacaacta aaaaagttgt tgaatacgct catgaaaaag gtgtttctgt
    12421 agaagctgaa ttaggtactg ttggtggaca agaagatgat gttgtagcag acggtatcat
    12481 ttatgctgat cctaaagaat gtcaagaact agttgaaaaa actggtatcg atgcattagc
    12541 gccagcatta ggttcagttc atggtccata caaaggtgaa ccaaaattag gatttaaaga
    12601 aatggaagaa atcggtttat ctacaggttt accattagta ttacacggtg gtactggtat
    12661 cccgactaaa gatatccaaa aagcaattcc atttggtaca gctaaaatta acgtaaacac
    12721 tgaaaaccaa atcgcttcag caaaagcagt tcgtgacgtt ttaaataacg acaaagaagt
    12781 ttacgatcct cgtaaatact taggacctgc acgtgaagct atcaaagaaa cagttaaagg
    12841 taaaattaaa gagttcggta cttcaaaccg tgctaaataa ttaatattta gtttttaagt
    12901 tattaataac gtagggatat taatttcaaa aaggagcaga cagaatggtg tttgcttctt
    12961 ttttatgttg ttaaggcacg aattgataca gcacatttta aaagttgaag ctaaaaatga
    13021 tgaaatatac aaaaaatgat tgtgctataa ttatatagaa aatgctgtag ttgatgaata
    13081 aaaacataca aaagcaatcc cactaaagct gaattggctg tcaaaataat gtcaatattt
    13141 gagcgaacaa tcagacttta gaccatggtc aagcgggtaa taacatcata tattaattaa
    13201 ggtgaatgat gcgttattaa tcaatcgtaa tagaaagaca agattttaac gattaaaata
    13261 aactatttta caaataaagt aaaattaatt tataatgcta ataatgcaaa aaattaaaaa
    13321 gtaatggaca aaggagataa atgatatggc tcaagaggta ataaaaataa gaggtggacg
    13381 cacactaaat ggggaagtta atatcagtgg cgcgaaaaac agtgctgtag ctatcattcc
    13441 tgcaacatta ttagctcaag gacatgtgaa attagaaggg ttaccgcaaa tctctgatgt
    13501 taaaacttta gtgagtttat tagaagattt aaatattaag gcatcactaa atggtacgga
    13561 attagaagtt gatacaactg aaatacaaaa tgctgcatta ccaaataata aagttgaatc
    13621 attacgtgct tcatactata tgatgggtac catgttaggt agatttaaaa aatgtgtgat
    13681 tggtttaccg ggaggatgtc cacttgggcc acgtccaatt gatcaacata ttaaaggatt
    13741 taaagcttta ggtgctgaaa ttgatgaatc aagtacgaca tcaatgaaaa ttgaagctaa
    13801 agagttaaaa ggtgcacata tttttctaga tatggttagc gtaggtgcaa caattaacat
    13861 tatgctagct gcagtttatg caacaggaca aactgtaatt gaaaatgctg ctaaagaacc
    13921 ggaagtagtt gatgttgcta acttcttaac aagtatggga gctaatatta agggagcggg
    13981 tacatcaacg attaaaatca atggtgtcaa agaattacat ggttctgagt atcaagttat
    14041 acctgataga attgaagcag gcacatatat gtgtatcgct gcagcatgtg gagaaaatgt
    14101 tatattaaat aatattgttc cgaaacatgt agaaacatta actgctaaat ttagtgaatt
    14161 aggtgtgaat gttgatgtaa gagacgaaag aattcgtatc aataataatg caccatatca
    14221 atttgttgat attaaaacac tagtatatcc aggttttgct actgatttac aacagcctat
    14281 tacaccatta ttatttatgg caaatggtcc ttcatttgta actgatacga tttatccgga
    14341 gcgttttaaa catgttgaag aattaaagcg catgggtgca aatattgaag ttgacgaagg
    14401 cacagcaacg ataaaaccat caacattaca tggtgctgaa gtttatgcta gcgatttaag
    14461 agcaggagct tgtttaatta ttgctggttt aattgctgag ggtgtaacta caatttacaa
    14521 tgtaaaacat atttatagag gttatacaga tattgtagaa cacttaaaag ctttaggtgc
    14581 agatatttgg acggaaactg tatagattta tcttgttttg tggtataata attatactga
    14641 ttaaaaaagg gtgttcgttt acattctaaa tttattgatg agaggtgaag atgatggaag
    14701 tatgtccgta tctcgaagaa acttttaaaa tacttggtag aagttggaat ggattaatca
    14761 ttaattatct ctcaagatgt aatgactgtt cagcacactt ttccgatatg aaaagagatt
    14821 tgaaaacaat aacgccacgt gctttaagtc ttaagttgtc agagcttgca caatgggagt
    14881 tagttgagaa gcaaatcatt tctacgagtc cagtacaaat tatttatgta ctaactgaaa
    14941 aaggtaaagc gttagcagag gctttacatc caattgaagc atgggcgcaa tcatatgtcg
    15001 atttaacaga tcaacgtact gctaaataat tacatgacgt ttaaacatag cattgattat
    15061 aactatttct aagtcttcgc attatttgcg atgatgaagg aatagttatt tttatttaaa
    15121 aatataaaaa attagatgca gcaaaatttt aaagcatttt attttgaaca tattaaaagg
    15181 gagcgtatca taatggaatg taatgtttat atcgtatgca ttacggataa ataatatata
    15241 aatcattctt gaggagtgaa agaataatga gagactacac aaagcaatac attaatggcg
    15301 aatgggtaga aagtaatagt aatgaaacga tagaagttat aaatccagca accgaagaag
    15361 taatcgggaa agttgctaaa ggtaataaag ctgatgttga taaagccgtc gaggcggcag
    15421 acgatgttta tttagagttc cgtcatacat ctgtgaaaga aagacaagcg ttattagata
    15481 aaattgtaaa agaatatgaa aacagaaaag acgatattgt acaagctatt acggatgaat
    15541 taggtgctcc tttatcatta tctgagcgtg tccattatca aatgggacta aaccattttg
    15601 ttgcagcgag agacgcatta gataactacg aatttgaaga acgccgcgga gatgatttag
    15661 ttgttaaaga agcaatcggt gtatctggct taattacacc gtggaacttc cctacaaacc
    15721 aaacatcatt aaaattagca gcagcatttg cggctggtag tccagttgta cttaaaccat
    15781 ctgaagaaac accatttgca gctgttattt tagctgagat ttttgataaa gtcggtgttc
    15841 ctaaaggtgt atttaacctt gttaatggtg atggtgctgg cgttgggaat cctttatctg
    15901 agcatcctaa agtacgcatg atgtcattta caggatcagg ccctactggt tctaaaatta
    15961 tggaaaaagc tgctaaatat tttaaaaagg tatcattaga gcttggtggc aaatcaccat
    16021 acatcgtcct agatgacgta gatattaaag aagcggctaa agcaacaaca ggcaaagttg
    16081 ttaataatac tggtcaagta tgtacagctg gtacacgtgt tttagtgcct aacaaaatta
    16141 aagatgcatt cttagctgaa ttaaaagaac aatttagcca agtgcgtgtc ggtaatccaa
    16201 gagaagatgg tacacaagta ggccctatca ttagtaaaaa acaatttgat caagtacaaa
    16261 attatattaa taaaggtatt gaagaaggtg ctgaattatt ttatggtggt cctggtaaac
    16321 cagaaggact tgaaaaagga tactttgcac gtccgacaat ttttattaat gtagataatc
    16381 aaatgacgat agcacaagaa gaaatttttg ggccagtaat gtcagttatc acttataacg
    16441 atttagatga agcgattcaa attgcaaatg atacaaaata tggtttggca ggatatgtta
    16501 ttggtaagaa caaagaaaca ttgcataaag tagctcgttc tattgaagca ggtacagtag
    16561 aaataaatga agcaggcaga aagccagact taccatttgg tggctataaa caatctggtt
    16621 taggtcgtga atggggcgat tatggtattg aagagttctt agaagtgaaa tctatagctg
    16681 gctattttaa ataaatttga tttttgaatt aaaaatcgca ataaaacagt gcacatgact
    16741 aattaagttt tgtgtactgt tttaattttg caatttttat aaatagattt tgtaattaaa
    16801 ataaaaattt gctatagtta ttcatgtatt taaaaggttg gggattagca taatgggatt
    16861 gtgctagcac agttatttat gcattgtcat gcctatctat tacttactaa ctaaaaaata
    16921 atgaaatggg tgtaaactat atgcctgaaa gagaacgtac atctcctcag tatgaatcat
    16981 tccacgaatt gtacaagaac tatactacca aggaactcac tcaaaaagct aaaactctta
    17041 agttgacgaa ctatagtaaa ttaaataaaa aagaacttgt tctagctatt atggaagcac
    17101 aaatggaaaa agatggtaac tattatatgg aaggtatctt agatgatata caaccagatg
    17161 gttatggttt tttaagaaca gtgaactatt ctaaagggga aaaagatatt tatatatctg
    17221 ctagccaaat tcgtcgtttt gaaattaaac gtggggataa agtaactggg aaagttagaa
    17281 aacctaaaga taacgaaaaa tattatggct tattacaagt tgactttgtt aatgaccata
    17341 acgcagaaga agtgaagaaa cgtccgcatt tccaagcttt gacaccactt tatccagatg
    17401 agcgtattaa attagagaca gaaacacaaa attattcaac gcgcatcatg gatttagtaa
    17461 caccgattgg tttaggtcaa cgtggtttaa tagtggcgcc acctaaagca ggtaaaacat
    17521 cgttattaaa agagatagcg aatgcaatca gtacgaacaa accagatgca aagctattta
    17581 ttttgttagt tggcgagcgt cctgaagagg taacagattt agaacgctca gtagaagctg
    17641 ctgaagtcgt tcattcaacg tttgacgaac caccagaaca ccatgttaaa gtagctgaat
    17701 tattacttga acgtgcaaag cgtttagtag aaattgggga agatgtcatt attttaatgg
    17761 attctataac gagattagca cgcgcttata acttagttat tccaccaagt ggtcgtacat
    17821 tatcaggtgg tttagatcct gcatctttac acaaaccaaa agcattcttc ggtgcagcga
    17881 gaaatattga agcgggtgga agtttaacaa tacttgcaac tgcattagtt gatacgggtt
    17941 cacgtatgga cgatatgatt tacgaagagt ttaaaggaac aggtaacatg gagttacatt
    18001 tagatcgtaa attgtctgaa cgtcgtattt tccctgcaat tgatattggc agaagttcaa
    18061 cgcgtaaaga agaattgttg ataagtaaat ctgaattaga cacattatgg caattaagaa
    18121 atctattcac tgactcaaca gactttactg aaagatttat tcgcaaactt aaaaggtcta
    18181 agaataatga agatttcttc aagcagctac aaaagtctgc agaagaaagt actaaaacgg
    18241 gtcgacctat aatttaataa acattatata ggggcttgcg ttttgaatta attaccttta
    18301 taattacaca gtattgggta aaaactcaca aataactctg ttccagatgg ttcagggcaa
    18361 aggagctgaa aataatgaaa caaggaattc atcctgagta tcaccaagta atctttttag
    18421 atactactac aaacttcaaa ttcttaagcg gttctacaaa aacatcttca gaaatgatgg
    18481 agtgggaaga tggaaaagaa tacccagtta ttcgtttaga tatttcatct gattcacatc
    18541 cattctacac tggacgtcaa aaattcgctg ctgcggatgg acgtgtggaa cgtttcaaca
    18601 aaaagtttgg tcttaaatca aacaactaat atagtaatcg ttaagctgtc tgcatcctat
    18661 aatggaattg tagacagctt ttttatttat aagcgtagta agggtatata cttacgactg
    18721 tgttcaaaga agtgactcaa taccatgttg ttaatacata atgataaatt tcccctttat
    18781 taaagtttgt attgagtaga aacaacgcac gaataagaag caacaaatta gaatagctgt
    18841 atataaatat gcaagacaaa tttataaaat aattgttcat gtacaatatc gatataatta
    18901 tgaaaaagac atttatgaat atttgaaata tgatataata aagcgattat gttttgaaaa
    18961 aggtggaact ctcaatgtat gaaacttacc attccggttg gatagaatgt attacaggta
    19021 gtatgtttag cggtaaatcc gaagaactaa taagacgtct aagaaggggt atatacgcta
    19081 aacaaaaagt cgttgtattt aaaccggcga ttgatgatcg atatcataaa gaaaaagtgg
    19141 tctctcataa tggtaatgcc attgaagcga ttaatatttc taaagcaagt gaaattatga
    19201 cgcacgactt aactaatgtt gatgttatag gaatagacga agtgcaattt tttgacgatg
    19261 aaattgtaag tatagtagaa aaactatcag cagatggaca ccgtgttatt gttgctggat
    19321 tagatatgga ttttaggggc gaaccgttcg aaccaatgcc taaattgatg gctgtcagtg
    19381 aacaagtaac gaaacttcaa gcagtttgtg ctgtttgtgg ttcttcatct agtagaacgc
    19441 aacgattaat aaatggtaaa ccagctaaaa tcgatgatcc gattatctta gtaggtgcca
    19501 atgaaagtta tgagccacgt tgtcgcgcgc accatatcgt agcacctagt gataataata
    19561 aggaggaatt ataagtgttt gatcaattag atattgtaga agaaagatac gaacagttaa
    19621 atgaactgtt aagtgaccca gatgttgtaa atgattcaga taaattacgt aaatattcta
    19681 aagagcaagc tgatttacaa aaaactgtag atgtttatcg taactataaa gctaaaaaag
    19741 aagaattagc tgatattgaa gaaatgttaa gtgagactga tgataaagaa gaagtagaaa
    19801 tgttaaaaga ggagagtaat ggtattaaag ctgaacttcc aaatcttgaa gaagagctta
    19861 aaatattatt gattcctaaa gatcctaatg atgacaaaga cgttattgta gaaataagag
    19921 cagcagcagg tggtgatgag gctgcgattt ttgctggtga tttaatgcgt atgtattcaa
    19981 agtatgctga atcacaagga ttcaaaactg aaatagtaga agcgtctgaa agtgaccatg
    20041 gtggttacaa agaaattagt ttctcagttt ctggtaatgg cgcgtatagt aaattgaaat
    20101 ttgaaaatgg tgcgcaccgc gttcaacgtg tacctgaaac agaatcaggt ggacgtattc
    20161 atacttcaac agctacagtg gcagttttac cagaagttga agatgtagaa attgaaatta
    20221 gaaatgaaga tttaaaaatc gacacgtatc gttcaagtgg tgcgggtggt cagcacgtaa
    20281 acacaactga ctctgcagta cgtattaccc atttaccaac tggtgtcatt gcaacatctt
    20341 ctgagaagtc tcaaattcaa aaccgtgaaa aagcaatgaa agtgttaaaa gcacgtttat
    20401 acgatatgaa agttcaagaa gaacaacaaa agtatgcgtc acaacgtaaa tcagcagtcg
    20461 gtactggtga tcgttcagaa cgtattcgaa cttataatta tccacaaagc cgtgtaacag
    20521 accatcgtat aggtctaacg cttcaaaaat tagggcaaat tatggaaggc catttagaag
    20581 aaattataga tgcactgact ttatcagagc agacagataa attgaaagaa cttaataatg
    20641 gtgaattata aagaaaagtt agatgaagca attcatttaa cacaacaaaa agggtttgaa
    20701 caaacacgag ctgaatggtt aatgttagat gtatttcaat ggacgcgtac ggactttgta
    20761 gtccacatgc atgatgatat gccgaaagcg atgattatga agttcgactt agcattacaa
    20821 cgtatgttat taggagagcc tatacagtat atagttggct ttgcctcatt ttatggtaga
    20881 acgtttgatg taaactcaaa ttgtttgata ccaagacctg aaaccgaaga agtaatgttg
    20941 catttcttac aacagttaga agatgatgca acaatcgtag atatcggaac gggtagtggt
    21001 gtacttgcaa ttactttgaa atgtgaaaag ccggatttaa atgttattgc tactgatatt
    21061 tcacttgaag caatgaatat ggctcgtaat aatgctgaga agcatcaatc acaaatacaa
    21121 tttttaacag gggatgcatt aaagccctta attaatgaag gtatcaagtt gaacggcttg
    21181 atatctaatc caccatatat agatgaaaaa gatatggtta cgatgtctcc aacggttacg
    21241 agattcgaac cacatcaggc attgtttgca gataaccatg gatatgctat ttatgaatca
    21301 atcattgaag atttacctca cgttatggaa aaaggcagcc cagttgtttt tgaaattggt
    21361 tacaatcaag gtgaggcact taaatcaata attttaaata aatttcctga caaaaaaatc
    21421 gacattatta aagatataaa tggccacgat cgaatcgtct catttaaatg gtaattagaa
    21481 gttatgcctt tgctatgatt agttaagtgc atagcttttt gctttatatt atgataaata
    21541 agaaaggcgt gattaagttg gatactaaaa tttgggatgt tagagaatat aatgaagatt
    21601 tacagcaata tcctaaaatt aatgaaataa aagacattgt tttaaacggt ggtttaatag
    21661 gtttaccaac tgaaacagtt tatggacttg cagcaaatgc gacagatgaa gaagctgtag
    21721 ctaaaatata tgaagctaaa ggccgtccat ctgataatcc gcttattgtt catatacaca
    21781 gtaaagatca attaaaagat tttacatata ttttagatgc acccgtagaa aagttaatgc
    21841 aggcattctg gccgggccct atttcgttta tattgccgtt aaagccaggc tatctatgtc
    21901 gaaaagtttc tggaggttta tcatcagttg ctgttagaat gccaagccat tctgtaggta
    21961 gacaattatt acaaatcata aatgaacctc tagctgctcc aagtgctaat ttaagtggta
    22021 gaccttcacc aacaactttc aatcatgtat atcaagattt gaatggccgt atagatggta
    22081 tcgttcaagc tgaacaaagt gaagaaggat tagaaagtac ggttttagat tgcacatctt
    22141 ttccttataa aattgcaaga cctggttcta taacagcagc aatgattaca gatatacttc
    22201 cgaatagtat cacccatgct gattataatg atactgaaca gccaattgca ccaggtatga
    22261 agtataagca ttactcaccc aatacaccac ttacaattat tacagatatt gagagcaaaa
    22321 ttggaaatga cggtaaagat tggtcttcta tagcttttat tgtgccgagt aataaggtgg
    22381 cgtttatacc aagtgaagcg caattcattc aattgtgtca ggatgacaat gatgttaaac
    22441 aagcaagtca taatctttat gatgtgttac attcacttga tgaaaatgaa aatatttcag
    22501 cggcgtatat atacggcttt gagctgaatg ataatacaga agcaattatg aatcgcatgt
    22561 taaaagctgc aggtaatcac attgttaaag ggtgtgaact atgaagattt tattcgtttg
    22621 tacaggtaac acatgtcgta gcccattagc ggaaagtatt gcaaaagagg ttatgccaaa
    22681 tcatcaattt gaatcaagag gtatattcgc tgtgaacaat caaggtgttt cgaattatgt
    22741 tgaagacata gttgaagaac atcatttagc tgaaacgacc ttatcgcaac aatttactga
    22801 agcagatttg aaagcagata ttattttgac gatgtcgtat tcgcacaaag aattaataga
    22861 ggcacaattt ggtttgcaaa atcatgtttt cacattgcat gaatatgtaa aagaagcagg
    22921 agaagttata gatccatacg gtggaacaaa agaaatgtat gtacatacct atgaagaact
    22981 tgtgagttta attttaaaat taaaagatat tatttgctag taggaattct gtataattag
    23041 tatatgttaa cagttaaata agttataata gaggagtgta gcactttgct atattccttt
    23101 tttattttta ggaagggtgg gcttttatga aagatttgac aatgttatta gacgaattaa
    23161 aagatatgtc cttttttaac aaaggagata tatgtttaat tggatgttca acatcggaag
    23221 tcattggcga aaaaattggg actgtcggtt cgatggaagt tgcagaaact atttttaatg
    23281 cattagatgt tgtgagtaaa gagacaggcg ttacttttgc ttttcaagga tgtgaacata
    23341 tcaacagagc gataacaata gaaaaatctc agtataatcc attaacaatg gaagaagttt
    23401 ccgtcgttcc aaatgtacat gctggaggga gtttggcaac atacgctttt cagcatatga
    23461 aggatccgat cgtggtcgaa catattactg ttccttgtgg aattgatatt ggtcaaacat
    23521 taataggcat gcatattaaa catgtatgtg tacctgtaag gacctcagta aagcaagtag
    23581 gacaggcaat tgtaactatt gccacatcta ggccgaaaaa gattggtggc gaacgtgcta
    23641 aataccaata agtttttttg aaagaagggg agattttatg tcttatatca ccaagcaaga
    23701 taaagttatc gcagaagcaa tcgagagaga atttcagaga caaaatagca acatagagtt
    23761 aatcgcatcg gaaaattttg tatcggaagc ggttatggaa gcacaaggtt cagtgttgac
    23821 taataagtat gctgaaggct atccaggacg ccgatattat ggtggctgtg agtttgtaga
    23881 tgttactgaa agcatcgcaa ttaatcgtgc aaaagcattg tttggagctg aacatgtcaa
    23941 tgttcaacca cattcaggtt cgcaagcgaa catggctgtt tacttagttg cattagaaat
    24001 gggcgacacg gttttaggta tgaatttgag tcatggtggt cacttgacac atggagcgcc
    24061 tgttaatttt agtggtaaat tctacaattt cgttgaatat ggagtagata aagacacaga
    24121 acgaatcaat tatgatgaag ttcgtaaatt agcgttagag cataagccta agcttattgt
    24181 ggcaggagca tcagcatatt caagaacaat tgacttcaaa aagtttaaag aaatcgcaga
    24241 tgaagtaaac gctaagttaa tggtagacat ggcacatatt gcaggattag tagcggcagg
    24301 tttacatcca aatccagtag aatatgctga ttttgtaaca actacaacac acaaaacatt
    24361 acgcggacca cgtggtggta tgattttatg taaggaagaa tataaaaaag acatagataa
    24421 aacaattttc cctggtattc aaggtggacc tcttgagcat gttattgcag caaaagcagt
    24481 tgcttttgga gaagcgttag aaaataattt caaaacgtat caacaacaag tggttaaaaa
    24541 cgcaaaagtt cttgcagaag cattaattaa tgaaggattt agaattgttt ctggcggtac
    24601 agataatcac ttagtagctg ttgatgtaaa agggtctata ggacttactg gtaaagaagc
    24661 tgaagagact ttagattcag ttggtatcac atgtaacaaa aataccattc cgttcgatca
    24721 agaaaaacct tttgtaacga gtggtatacg tttaggtaca cctgctgcaa caacgcgtgg
    24781 atttgatgaa aaagcttttg aggaagttgc aaaaatcatc agtttagcat tgaaaaatag
    24841 taaagatgaa gaaaaattac aacaagctaa agaacgcgtt gcgaaattaa cagctgaata
    24901 tcctctatat caataaacat acacaaagat aggagtaatt tttatgagta aagtacacgt
    24961 tttcgatcat ccattaattc aacacaaact aagctatatt agagatgtaa atacaggtac
    25021 taaagaattt agagaacttg tagacgaagt tggtatgttg atggcatatg aagtaactag
    25081 agacttagag cttcaggatg ttgatattga aacgcctgta actaaaatga cggctaaacg
    25141 attagctggt aaaaaattag ctatcgttcc aattttaaga gctggtttag gtatgactga
    25201 tggtatttta agtttagtgc ctgcagccag agttggacat atcggtttgt atcgagatcc
    25261 cgaaacatta aaggctgtag agtattttgc taagttacct caagatatta ctgaaagaca
    25321 aatcattgtt gttgatccta tgttagcaac aggcgcatca gcaattgaag ctattacttc
    25381 attgaagaaa cgtggcgcta aaaatattcg cttcatgtgt ttaattgcag caccagaagg
    25441 cgtagaaaag atgcatgaag cacatccaga tgtagatatt tatattgctg cacttgatga
    25501 aaagctaaat gataaagcat atatcacacc agggttaggc gacgctggag atagattgtt
    25561 tggtactaaa tagaagaatc ggagataagt agtttgatga aaaagattat gaccatattt
    25621 ggaacaagac ccgaagccat aaagatggct cctttagtta aagctttgga acaagaaaaa
    25681 atgctcgagc caattgttgt tgttacagca cagcatagag aaatgcttga ttcagtatta
    25741 agtacctttg aaatcaaacc aaaatacgat ttgaatatta tgaaatcagg acagacactt
    25801 tctgagataa cttctaaatc aatcactcaa ttagaacaag tgattcaatt agaaaaacca
    25861 gatatggtac ttgtacatgg tgatacgatg acgacatttg caggtggttt agcggcattt
    25921 tataaccaag tgccgattgg acacgttgaa gcagggctta gaagttatga taaatattca
    25981 ccttttccag aagaggtgaa tagacaatta gttggtgtat tagctgactt acattttgca
    26041 cctactaaaa atgctgcatc gcatttgtta agtgaaggga aatattcaga aagtgttgtt
    26101 gtcactggta atacagcgat tgatgctatg aaatatacag ttgatgacaa ttataaatct
    26161 aacatcatgg ataaatatca tgacaaaaaa ttcattctta tgacagcaca ccgacgagaa
    26221 aatatcgggc aacccatgga aaatatattt aaagcggtta gacgcttaat agatgagtat
    26281 actgatttag cactagtata tccaatgcat aaaaacccga aagttcgaga ggtggctcaa
    26341 aagattttag gtagtcatga cagaattgaa ttgattgaac ctttggatgt agtagatttc
    26401 cataattttg ctaaaaaatc ttattttatt ttgacagatt caggtggaat tcaggaagaa
    26461 gcgccatcat ttaataagcc agttttagtt ttgcgaagcg ttacagaacg tcctgaaggt
    26521 gttgaagctg gcacattaaa agtcattgga acaaataagc aaaatgtgta tcaagctgca
    26581 aaagaattaa tcgatgatga gagattatat catcaaatgt ctgaagcatc taatccttac
    26641 ggtgatggat ttgcttcaga aagaatagtt aatcatatta agtattattt gaatttaatc
    26701 actgaaaaac caagcgattt ctaagaaaaa tagacttttt gtctaattgt gtctaaattg
    26761 tgtctgaaca tgcttatttt tttacaacaa tttgaataga attgacacca caaatcgcct
    26821 atattatcat ttagtttgta tgagtggaac ggttttcatg attgatatca gtcacacgga
    26881 ggcatataaa atgagtcgtt tctacaacat ttttaaacag ttcattcaat attatttgta
    26941 tttattaata atattgggag gattatacct ttttacacac catgcattta tcttaggatt
    27001 aatcattggt gttactggtt ctgcaatcaa tactgttata tttgagagct acttagccaa
    27061 agctaaaaga ccagacacta tgcatatttc aactggaaat atgtggcgat acttagttgc
    27121 aattattgcc tgtatgattt ggtactttaa taaatcgcat gtaagtataa tcggtataat
    27181 tattggttta atgatttcat atgttgtagt tatcatacgt cctttactaa aggtgagcaa
    27241 ataaattaag aaagaggtga gattatggat cacaaatccc cgctcgtgag ttggaattta
    27301 ttcggttttg atatcgtttt caatttatca agtatattga tgatacttgt tacggcgttt
    27361 cttgtttttc tacttgctat catttgtacg cgtaatttga aaaaaagacc aactggcaaa
    27421 caaaatttcg ttgaatggat ttttgatttc gtgaggggaa tcattgaagg taacatggct
    27481 tggaaaaaag gtggtcaatt ccacttctta gcagtaacgc tgattctgta catttttata
    27541 gctaatatgt taggtcttcc gttttctata gtaacgaaag atcacacatt gtggtggaaa
    27601 tcaccaacag ctgatgcaac agtgacttta acgttgtcta caacgataat actgttaact
    27661 cacttttatg gaattaaaat gcgtggtacg aaacaatatc ttaaaggtta tgtacagccg
    27721 ttttggccat tggcaattat taatgttttt gaagagttca cttcaacatt aacgcttggt
    27781 ctgcgtttgt acggtaacat atttgcaggt gagatactat taacattact tgctggctta
    27841 ttctttaacg aaccagcatg gggttggatt ataagtatcc caggattaat tgtttggcaa
    27901 gcattttcaa tatttgtagg aacaatccaa gcatatatct ttattatgct ttcgatggtt
    27961 tatatgtcac ataaagtggc agatgaacac taaaaaattt caataattat atacaatcac
    28021 aggaggaaat taaattatga atttaatcgc agcagcaatc gcaattggtt tatcagcatt
    28081 aggagcaggt atcggtaacg gtttaatcgt ttcaagaaca gttgaaggtg tagcacgtca
    28141 accagaagca cgtggtcaat taatgggtat catgttcatt ggtgtaggtt tagttgaggc
    28201 attacctatc atcggtgtag taattgcatt catgacattt gctggataat taacagataa
    28261 aagaggtcgg gacaaagcgc ataggacata attcatgacg catatatagt aatatctttg
    28321 aactttatta aatagttgag atatgaacgc accatgccta tcgcataaat tcagtaggtc
    28381 ctaacctcgt cgtttttttc tatataacac tagcgattat tttaatgaaa ggagtgtcat
    28441 gaacccgtga ctgaaacagc taacttattc gttcttggtg cagctggagg cgttgagtgg
    28501 ggtactgtga ttgtacaggt cctaactttc atcgtgttac ttgcgttact taaaaagttc
    28561 gcatggggtc cattgaaaga tgtaatggat aaacgtgaaa gagatattaa cagagatatc
    28621 gatgacgcag aacaagctaa gttaaatgca cagaaacttg aagaagaaaa taaacaaaaa
    28681 cttaaagaaa cacaagaaga agttcaaaag attttagaag atgctaaggt tcaagcacgt
    28741 caacagcaag aacaaattat tcatgaagca aacgtacgtg caaacggtat gattgaaaca
    28801 gcacaaagtg aaatcaatag ccaaaaagaa cgtgccattg cagatattaa taatcaagta
    28861 tctgaactat cagtgttaat tgcttctaaa gttcttagaa aagaaatttc tgaacaagac
    28921 caaaaagcat tggttgacaa gtatctaaaa gaggcaggcg ataaataatg gtaaaagtag
    28981 ctaacaagta tgctaaagca ttatttgacg tgtcattaga tacaaataat ttagaaacta
    29041 ttaatcaaga attaacagtt ataaatgaag cagtaaaaga taaaattgag caattgaaaa
    29101 tggttgacag taatccaact caaacagcag aacaacgtcg tgagttgatt aatggtgtat
    29161 ttactgacat taatccatac attaaaaata tgatgtacgt tttagcagat aatagacata
    29221 tctcattaat agctgacgta ttcaaggcgt tccaaagctt atataacgga cactacaatc
    29281 aagattttgc aacaattgag tcaacatatg aattgagtca agaagagtta gataagattg
    29341 tcaaactagt aactcaacaa acgaagttat ctaaagttat tgtagataca aaaattaatc
    29401 cagatttaat tggtggattt agagttaaag tcggcacaac tgtattagat ggtagtgtta
    29461 gaaatgatct tgtccaatta caaagaaaat ttagaagagt taattaatta taaagaggag
    29521 tgacatagat ggccataaaa gctgaagaaa tcagtgcatt acttcgctca caaattgaaa
    29581 attatgagtc agaaatgtcc gtaactgatg taggtactgt attacaaatt ggtgatggta
    29641 ttgcattaat tcacggatta aatgacgtta tggctggtga gctagtagaa ttccataacg
    29701 gcgtacttgg tttagcccaa aaccttgaag agtcaaacgt gggtgtggtt attttaggac
    29761 catatacagg tattactgaa ggtgacgaag ttaaacgtac tggtcgtatc atggaagtac
    29821 cagtaggtga agaactaatc ggaagagttg ttaatccatt aggacaacct attgatggac
    29881 aaggaccgat taacacaact aaaacacgtc cagtagagaa aaaagctact ggtgtaatgg
    29941 atcgtaaatc agtagatgag ccattacaaa caggtatcaa agcaattgat gctttagtac
    30001 caattggtag aggtcaacgt gagttaatca tcggtgaccg tcaaacaggt aaaacaacaa
    30061 ttgcaattga cacaattttg aaccaaaaag atcaaggtac gatttgtatc tatgttgcta
    30121 ttggtcaaaa agattcaaca gtaagagcaa atgttgaaaa gttaagacaa gcaggcgctt
    30181 tagactacac tattgttgta gcagcatcag cttctgaacc ttctccatta ttatatattg
    30241 caccatattc aggtgtaaca atgggtgaag aattcatgtt taacggtaaa catgttttaa
    30301 tcgtttatga tgatttaact aaacaagcag cagcttatcg tgagttatca ttattattac
    30361 gtagacctcc aggtcgtgaa gcatacccag gtgacgtatt ctacttacat agtagattat
    30421 tagaaagagc agcaaaatta aacgatgact taggtggcgg ttcaattact gcattaccaa
    30481 ttatcgaaac acaagctggt gatatttcag cttatgtacc aacaaacgtt atttcaatta
    30541 cagatggaca aatcttctta caatctgatt tattcttctc aggtgtaaga ccagcgatta
    30601 atgccggaca atctgtatct cgtgttggtg gatctgcaca aattaaagca atgaagaaag
    30661 ttgctggtac gttacgtctt gacttagcgt catacagaga acttgaatca tttgcacaat
    30721 tcggttcaga ccttgatgaa tttactgcaa gtaaattaga acgtggtaaa cgtactgttg
    30781 aagtcttaaa acaagatcaa aacaaaccat taccagttga acatcaagtg ttgattattt
    30841 atgcattaac aaaaggatat ttagatgata ttccagttgt agatatcaca cgttttgaag
    30901 acgagttaaa ccactgggca gaatcaaatg ctactgaact gttaaatgaa atcagagaaa
    30961 ctggtggctt accagatgct gagaagtttg acacagcaat taacgaattc aaaaaaagct
    31021 ttagcaaatc tgaataataa acaagtttag tataaggtgg tgagatagtg gcttctctta
    31081 aagaaataga tactcgaata aaatcaacca aaaaaatgaa gcagattacg aaagcgatga
    31141 acatggtatc aagttcaaaa cttcgtagag ctgaaaaaaa tacaaaacaa ttcacaccat
    31201 atatggataa aatgcaagat gcaattactg cagttgcagg ggcaagtagc aatacaaacc
    31261 atccaatgct aagacctcgt aaaattacta gaagtggata tttagttatc acgagtgata
    31321 aaggtttagc aggtgcatat agtgcaaacg tgcttaaaaa attgattact gatattgaag
    31381 cgaaacatca agatagtagc gaatacagta ttgtagtttt agggcaacaa ggtgttgatt
    31441 tcctaaaaaa tagaggttat gacattgagt attctcaagt agacgtacct gatcaacctt
    31501 ctttcaaatc tgttcaagca ctagctaacc atgctataga cttatacagt gaagaagaaa
    31561 ttgatgaatt aaatatatac tatagtcatt atgtcagcgt tcttgaaaac aagcctacat
    31621 ctagacaagt attaccatta tctcaagagg attctagtaa gggacatggt catttgtctt
    31681 cttatgaatt tgagccagat aaagaatcta tcttaagtgt aatcttgcct caatatgttg
    31741 agagtttgat ttacggaaca atattagacg caaaagcaag tgagcatgca acacgtatga
    31801 ctgcgatgaa aaatgccact gataatgcaa ctgaacttat tgatgactta tcattagaat
    31861 ataacagagc gagacaagct gaaattacgc aacaaattac tgaaattgtt ggtggttccg
    31921 cagcgcttga ataatattta aaggaggaaa atagcatggg aattggccgt gtaactcaag
    31981 ttatgggtcc tgtaattgat gttcgatttg aacataacga agttcctaaa attaataacg
    32041 ccttggttat tgatgtgcct aaagaagaag gtacaataca actaacatta gaagttgcgc
    32101 tgcaattagg tgacgacgtt gttcgtacaa ttgcgatgga ttcaactgat ggtgtccaaa
    32161 gaggcatgga tgtaaaagat acaggcaaag aaattagtgt acctgttggt gacgaaacat
    32221 taggtcgtgt atttaatgta ctaggtgaaa caattgacct taaagaagaa attagtgatt
    32281 ctgttcgccg cgatcctatc catcgtcaag caccagcatt cgatgaactt tcaacagaag
    32341 ttcaaatttt agaaacaggt attaaagtag tagatttact agcaccttat attaaaggtg
    32401 gtaaaatcgg attgttcggt ggtgccggtg taggtaaaac agtattaatc caagaattaa
    32461 ttaacaacat cgctcaagag cacggtggta tttctgtatt cgccggtgta ggtgaacgta
    32521 ctcgtgaagg taacgattta tacttcgaaa tgagtgacag tggtgtaatt aagaaaacag
    32581 ccatggtatt cgggcaaatg aatgagccac ctggtgcacg tatgcgtgtt gcattatctg
    32641 gtttaacaat ggctgaatat ttccgtgacg aacaaggtca agacgtatta ttattcatcg
    32701 ataacatttt cagatttaca caagctggtt ctgaggtatc tgcattatta ggtcgtatgc
    32761 cttctgcagt aggttaccaa ccaacacttg ctactgaaat gggacaatta caagaacgta
    32821 ttacgtctac aacaaaagga tcagttactt ctattcaagc ggtattcgta cctgccgatg
    32881 actatactga cccagctcca gcgacagcgt ttgcccattt agatgcaact acaaacttag
    32941 aacgtaaatt aactgaaatg ggtatttatc cagccgtgga tccattagcg tctacatcaa
    33001 gagcattgga accatcaatt gtaggtcaag aacattatga agtagctcgt gatgtacaat
    33061 caacacttca aaaataccgt gaattacaag atatcattgc tatcttaggt atggacgaat
    33121 tatctgatga agataaacaa acagttgaac gcgcacgtag aattcaattc ttcttatctc
    33181 aaaacttcca cgtagcggaa caatttactg gtcaaaaagg ttcttatgta cctgttaaga
    33241 caacagttgc aaactttaaa gatatcttag atggtaaata tgaccatatt ccagaagatg
    33301 ctttccgttt agttggtagc atggatgatg ttattgcaaa agctaaagat atgggtgttg
    33361 aagtataaca attaggagga atggataatg aatacattaa acctagatat tgtcactcct
    33421 aatggttctg tttacaatcg tgataatgtt gaactcgttg ttatgcaaac aacagctggt
    33481 gagataggtg tcatgagtgg acatattcca actgtagctg ctttaaaaac aggctttgta
    33541 aaagtgaaat ttcacgatgg aactgaatat attgctgtaa gcgatggctt tgttgaagtt
    33601 agaaaagata aagtttcaat cattgttcag actgcagaaa ctgcaagaga aattgatgtt
    33661 gaaagagcta aattagccaa agcaagagca gagtctcact tggaaaatga tgacgacaat
    33721 actgatattc atagagccga aagagcttta gagagagcaa ataaccgttt gcgtgtggct
    33781 gaattaaaat agtaaataaa gggtcgaaga tgtgatttca tatcttcgac ccttttttga
    33841 attatattga tttaaagata caaaacatga gagggggaag gaattgataa agaaccatta
    33901 aagatttatg atgtagtggt tctttatcat taaacacagc taatgtgtat ttaaaaatag
    33961 gaatacatga gtaaaactca tgtataagaa atactaattt ctaaagaaaa agtatttctt
    34021 tatgttgggg cccaccccaa cttgcattgt ctgtataaat tggtgttcca atttctttat
    34081 gttggggccc cacccccaac ttgcacatta atgtaagctg actttccgcc agcttctgtg
    34141 ttggggcccc gcaaactact gccaaatata acactataga gtctagacgt tgatttatgt
    34201 ccgactccca aaaatagttt tactttttac aatcactact agattgctaa aatcaaaatt
    34261 tccttcacca ctatctacag tcgacatttc attttttgaa agtagctaca ttttttcatg
    34321 ccaagatact ttatagttat gatatttatg taaaaagaat tatatagtaa gttagcttaa
    34381 actttactaa aaacgggtat taaactttgt atcattattt aaatttttca tgtacaatgt
    34441 aatacagtaa tcttatgagg tgataaaatg gattatatcg gacaatatgc agttatccat
    34501 ttagtgttac atgttgtatg tatttgtatt gcctattggg ctttacaatc aattagatta
    34561 gatcaatttt ttaaaaaggg atacgccact caattacaag tgtgtatgat atttgttgct
    34621 attttattag gcactgcagt aagcaatttt attgtagatt tgttacaata ctcgacgcag
    34681 gtaaaatatt taataaaata agtctaactc tatgatttgt aatcaaaact agatataatt
    34741 aaataatgac ttaaaataat tttaaaatag ggaaatgtaa agtaatagga gttctaagtg
    34801 gaggatttac gatggataaa atagtaatca aaggtggaaa taaattaacg ggtgaagtta
    34861 aagtagaagg tgctaaaaat gcagtattac caatattgac agcatcttta ttagcttctg
    34921 ataaaccgag taaattagtt aatgttccag ctttaagtga tgtagaaaca ataaataatg
    34981 tattaacaac tttaaatgct gacgttacat acaaaaagga cgaaaatgct gttgtcgttg
    35041 atgcaacaaa gactctaaat gaagaggcac catatgaata tgttagtaaa atgcgtgcaa
    35101 gtattttagt tatgggacct cttttagcaa gactaggaca tgctattgtt gcattgcctg
    35161 gtggttgtgc aattggaagt agaccgattg agcaacacat taaaggtttt gaagctttag
    35221 gcgcagaaat tcatcttgaa aatggtaata tttatgctaa tgctaaagat ggattaaaag
    35281 gtacatcaat tcatttagat tttccaagtg taggagcaac acaaaatatt attatggcag
    35341 catcattagc taagggtaag actttaattg aaaatgcagc taaagaacct gaaattgtcg
    35401 atttagcaaa ctacattaat gaaatgggtg gtagaattac tggtgctggt acagacacaa
    35461 ttacaatcaa tggtgtagaa tcattacatg gtgtagaaca tgctatcatt ccagatagaa
    35521 ttgaagcagg cacattacta atcgctggtg ctataacacg tggtgatatt tttgtacgtg
    35581 gtgcaatcaa agaacatatg gcgagtttag tctataaact agaagaaatg ggcgttgaat
    35641 tggactatca agaagatggt attcgtgtac gtgctgaagg ggaattacaa cctgtagaca
    35701 tcaaaacact accacatcct ggattcccga ctgatatgca atcacaaatg atggcattgt
    35761 tattaacggc aaatggtcat aaagtcgtaa ccgaaactgt ttttgaaaac cgttttatgc
    35821 atgttgcaga gttcaaacgt atgaatgcta atatcaatgt agaaggtcgt agtgctaaac
    35881 ttgaaggtaa aagtcaattg caaggtgcac aagttaaagc gactgattta agagcagcag
    35941 cagccttaat tttagctgga ttagttgctg atggtaaaac aagcgttact gaattaacgc
    36001 acctagatag aggctatgtt gacttacacg gtaaattgaa gcaattaggt gcagacattg
    36061 aacgtattaa cgattaattc agtaaattaa tataatggag gatttcaacc atggaaacaa
    36121 tttttgatta taaccaaatt aaacaaatta tacctcacag acagccattt ttattaattg
    36181 ataaagtagt tgaatatgaa gaaggtcaac gttgtgtggc tattaaacaa gtatcaggaa
    36241 acgaaccatt ctttcaaggg cattttcctg agtatgcggt aatgccaggc gtattaatta
    36301 ctgaagcgtt agctcaaaca ggtgcggtag ctattttaaa tagtgaagaa aataaaggta
    36361 aaatcgcttt atttgctggt attgataaat gtcgttttaa acgtcaagta gtacctggtg
    36421 atactttaac gttggaagta gaaatcacta aaattaaagg accaatcggt aaaggtaatg
    36481 ctaaagctac tgtcgatggt caacttgctt gtagttgtga acttacattt gcaattcaag
    36541 atgtaaaata aaacaaaaaa acattcaaag atttaatgtg ttggcataat ctttgaatgt
    36601 tttttatttt actcttctaa tttttcatcc tttaactttg gtttagactg catcattcga
    36661 ttaaatgatt tttttaattc ttcaccagat aatccatcat caataagttg ttctaataaa
    36721 ctttcagcat actgttgacg taaagtgtaa atatgacatt caaatacgat agacattgtt
    36781 gttttaaaat ttttaatact tttcacagta gattcaaata aagctggatc attggctgga
    36841 cgcgtgatga cgacacgaat atccaataac tgttcactgt cttgatattc cttaaatgtt
    36901 tcataatgct cattagaaat gacaatttca agcaaccaac ccgtgccact ttcctcttta
    36961 ttaattatta cgccatcttc taattcatat tctataattc tgccgtgttc attcacaatt
    37021 tgaaacctta ctgctttaaa agttttcata ttcccacatc ccattagttt aatatttatg
    37081 atttttgaat tatttagaag atttaaggtc atttcatgac taatttcttt aaatataaca
    37141 aataacgaga tgctatagct attaattata taaaatttgc cattttgacc atttttaagc
    37201 tagtaattaa gatataggca taggaggtga atcacacact acatgctaaa taaaatcgta
    37261 attgtcggga gactgacgaa agacgcacaa atatttgaaa aggaggatag aaaaattgca
    37321 acgttttgtg ttgcaacgca ccgaaattat aaagatgaaa atggagaaat cgtctgtgat
    37381 tacttattct gtaaagcatt tggcaagtta gcttctaata tagaaaaata tactaatcaa
    37441 ggtacattgg ttggtataac tggtcaaatg agatcaagaa agtatgataa agacggacaa
    37501 acacactttg tcactgaatt atatgttgaa acaataaaat ttatgtcccc taaatcccaa
    37561 aataatgaaa ttctctcaga tagtatttta gatattgact ctcaaaatat agataatcat
    37621 gacttattag aaatttaaat ccaattatcc taaacatcct taatatacat ttaatccatc
    37681 ctaaattttt tatagatcta attaacttga aatatactca cttaaaaacg acttacatga
    37741 ctagtactgt tgtgtaggtc gtctttttgt gtagtttttc taatagaaaa tagacttaga
    37801 ttgcaaaacg attacataaa aaattgcaaa tgaaaaacat ttttactgtc atgacaattc
    37861 tcgatagaat catatttgaa attgtggggg atttcgttgt aatcactgtg taaaaaacaa
    37921 ggctattttt ttattttgtt gtaacctgca cggattttac agatgttaca gtagtctctg
    37981 taaaactttt agagaaacaa attacttgta ggaggatttt aagattatga agaaaacatt
    38041 actcgcatca tcattagcag taggtttagg aatcgtagca ggaaatgcag gtcacgaagc
    38101 ccatgcaagt gaagcggact taaataaagc atctttagcg caaatggcgc aatcaaatga
    38161 tcaaacatta aatcaaaaac caattgaagc tggcgcttat aattatacat ttgactatga
    38221 agggtttact tatcactttg aatcagatgg tacacacttt gcttggaatt accatgcaac
    38281 aggtgctaat ggagcagaca tgagtgcaca agcacctgca actaataatg ttgcaccatc
    38341 agctgttcaa gctaatcaag tacaatcaca agaagttgaa gcaccacaaa atgctcaaac
    38401 tcaacaacca caagcatcaa catcaaacaa ttcacaagtt actgcaacac caactgaatc
    38461 aaaagcatca gaaggttcat cagtaaatgt gaatgatcat ctaaaacaaa ttgctcaacg
    38521 tgaatcaggt ggcaatattc atgctgtaaa tccaacatca ggtgcagctg gtaagtatca
    38581 attcttacaa tcaacttggg attcagtagc acctgctaaa tataaaggtg tatcaccagc
    38641 aaatgctcct gaaagtgttc aagatgccgc agcagtaaaa ttatataaca ctggtggcgc
    38701 tggacattgg gttactgcat aagccattta tgcatagcta atcaatagtt atataagtaa
    38761 cttttagatt ggaatatatc ggggaattct cgatatgttc cggtcttttt tatttcgcaa
    38821 atatatgtca ttatagaatt attatttaaa gataataata caactgagca acaataattg
    38881 aaaatgttga aaccgtttgc taagattaat ttgcaattga atactaagga actactaggg
    38941 gagcctaatg atatggctga gatgaattgt tcagaccctt atgacctgat ttggttagta
    39001 ccaacgtagg aaagtagtta ttgaagtgat ggggctagta tagcaacatt gccatcaaac
    39061 tactttcatg ttggaagtag tttttttatt tagggaggat tttttacaat ggaattttca
    39121 caaaaattgt accaagctgc aaagccaatc attaatgata tttatgagga tgactttatt
    39181 caaaaaatgt tattaggcaa tatacaagct gatgcattac gtcactattt acaagctgat
    39241 gcagcttatt taaaagaatt cacaaactta tatgctttgt taattccaaa aatgaatagc
    39301 atgaatgacg taaaattttt agtagagcaa atagagttta tggttgaagg tgaagtactt
    39361 gcgcatgata ttttagcgca aattgttggt gaatcatacg aagaaatcat taaaacaaaa
    39421 gtttggcctc caagtggcga ccattacaga aaacatatgt atttccaagc gcatagtcgt
    39481 gaaaatgcta tttatacaat tgctgcaatg gcaccttgtc catatattta tgcagaatta
    39541 gctaagcgct ctcaaagtga tcataagctt aatagagaaa aagatacggc aaaatggttt
    39601 gatttttata gtactgaaat ggatgacata attaacgtat ttgaggcatt aatgaataaa
    39661 ttagccgaat caatgtctga caaggaattg gaacaagtaa agcaagtatt tttagaaagt
    39721 tgcatacatg agcgtcgatt tttcaatatg gctatgacac tagaacaatg ggaatttgga
    39781 ggaaaagtaa atgattaaac ctaaaatagc attaaccatt gcaggtactg atccaacagg
    39841 tggtgccggc gtaatggctg atttaaaatc atttcattca tgtggtgtat atggtatggg
    39901 cgtcgttaca agtattgttg ctcaaaatac attgggcgta caacatattc ataatttaaa
    39961 tcatcaatgg gtagatgaac aacttgatag tgtcttcaac gataccttac cgcatgctat
    40021 taaaacgggg atgattgcta cagcagatac tatggaaacg attcgtcatt atttaatgca
    40081 acatgaatct attccatatg taattgatcc tgttatgttg gcgaaaagcg gtgattcatt
    40141 aatggataat gacacaaagc aaaacttgca gcatacgtta ttgccattag ctgacgtagt
    40201 aacaccgaat ttaccagaag ctgaagaaat aacgggacta accattgata gtgaagaaaa
    40261 aattatgcag gctggccgca tctttattaa tgagattggt agtaaaggtg tcatcattaa
    40321 aggcggtcat tcaaatgata ctgatatagc aaaagattat ttatttacta acgaaggtgt
    40381 tcaaacattt gaaaatgaac gatttaaaac aaaacatacg catggaacag ggtgtacatt
    40441 ttcagcagtt ataacggcag aacttgcaaa aggtagacca ttatttgagg ctgtacacaa
    40501 ggctaaaaag tttatttcaa tgagtataca atatacgcct gaaatcggcc gtggtagagg
    40561 tccagtgaat cattttgcat atttaaagaa agagggatta gacgatgaat tatctaaata
    40621 aaatacgtat tgaaaaccca ttaacaattt gttatacaaa cgatgtagtt aaaaatttta
    40681 cagcgaatgg tttattaagt attggtgcta gccctgcaat gagtgaagct cccgaagaag
    40741 ctgaagaatt ttacaaagtt gcacaagcgc tattaatcaa tatcggtact ttaacagcag
    40801 aaaatgaaca agatattatt gcgattgctc aaacggcaaa tgaggcaggc ttacctattg
    40861 tatttgaccc tgtagctgtt ggtgcttcta catatcgaaa gcaattttgt aaattattat
    40921 tgaaatcagc gaaagtatca gtaattaaag gcaatgcatc tgaaatatta gcgttgattg
    40981 atgatacagc aactatgaaa ggtacagata gtgatgctaa tcttgatgcg gttacaatag
    41041 cgaaaaaggc ttacgcaaca tataaaactg caatagtaat cacaggtaaa gaggacgtta
    41101 ttgttcaaga taataaagcc atcgtattag ctaatggatc tccattatta gcacgagtaa
    41161 ctggagctgg ttgtttatta ggaggcgtta ttgctggatt tttatttaga gaaacagaac
    41221 cagacataga agcgttaatt gaagcggtaa gcgtatttaa tattgctgct gaggtagctg
    41281 ctgaaaatga aaattgtggt ggtcctggta cgttttcacc attgttgctt gatacgttat
    41341 atcatttaaa tgaaacaacc tatcaacaac ggattcgtat tcaagaggtg gaataatatg
    41401 tttaaccaat cgtatctaaa tgtgtatttt atttgtggga cctccgatgt tccgagtcat
    41461 cgaactattc atgaagtatt agaagcagca ttaaaggcag ggattacact tttccaattt
    41521 cgagaaaagg gtgaatcagc tttaaaagga aacgataaac ttgtattagc taaagaatta
    41581 cagcatctct gtcatcaata tgatgtgcct tttatcgtta atgatgatgt atccttggca
    41641 aaagaaatta acgcggatgg tatacatgtc ggtcaagatg atgcgaaagt caaagagata
    41701 gcacaatatt tcactgataa aattattgga cttagtatta gtgatttaga tgagtatgca
    41761 aaatctgatt taactcatgt tgattatata ggtgttggac caatctatcc tacgccgtcg
    41821 aagcatgacg cgcatatacc agtaggtcca gaaatgattg caacgtttaa agaaatgaat
    41881 ccgcaactac cgattgtagc tattggcggt atcaatacta ataatgtggc gccgattgta
    41941 gaagcaggtg ccaatggaat ttcagtcatt tcagccattt ctaaaagtga aaatattgaa
    42001 aagactgtta atcgattcaa agattttttt aataattagt taaatttttc cacttattgt
    42061 ttcatacgta ctcattaatg tgatagaata aatcctatgt gaatataatg atagaggtgg
    42121 aaaaatgaag aaaaaagcgt tactaccatt atttttaggt attatggtct ttttggctgg
    42181 ttgtgactat tctaaacctg aaaaacgtag tgggtttttc tacaatacat tcgtagatcc
    42241 aatgaaaaat gtattggatt ggttgggaaa taacttatta aacgacaatt atggtttagc
    42301 tattattatc cttgtattgg taattcgtat tattttatta ccattcatgt tgtcaaacta
    42361 taaaaatagt catatgatgc gtcaaaaaat gaaagttgca aagccagaag ttgaaaaaat
    42421 tcaagaaaaa gtgaaacgtg cgcgtacaca agaagaaaaa atggctgcaa accaagaatt
    42481 aatgcaagta tataaaaagt atgacatgaa cccgattaag agtatgttgg gttgtttacc
    42541 aatgctaatc caattgccaa tcatcatggg attatacttt gtacttaaag accaacttgt
    42601 agatggtttg tttaaatatc cacacttctt atggttcgat ttaggacgtc ctgatatttg
    42661 gattacaatt attgccggtg ttttatactt tatccaagca tatgtatcaa gtaaaacgat
    42721 gccagacgaa caacgtcaaa tgggttacat gatgatggtc atttcaccaa ttatgattat
    42781 ctggatttca ttaagctcag catcagcact tggtttgtac tggtcagtca gtgcggcgtt
    42841 ccttgtagtt caaacacact ttgcgaacat ttattatgaa aaagtcgcta aaaaagaagt
    42901 acaacctttc attgaagcgt atgaaagaga gcacaacggc ggcagcaata aaaaaggcaa
    42961 aaacacacaa gtagtgtcta aaaagaaaaa gaaataacct tagcaagtcc aaataatgtg
    43021 caccctaaaa gttgaagata attttaactt tatagggcgc acattatttt tcgcttcaat
    43081 agcgaaactt agtgtcacat tatacaatat gtcataaata gaaacagcag taagatgatt
    43141 tcaaatgtga aagtcatcct actgctgttt ttatttgaaa taatattaac gagatttcag
    43201 tatgtaaaaa gtggttaata acagagttgt cattcttcat cgcaatgtca tcttgacaca
    43261 atgattcatc agtgtagaag tatatgtcgt aacgcaacat gtccacagca attcactaat
    43321 gcatgtaaca gtgtgaaata ataacgtcac aacggcataa tacacattgc aacgtcccaa
    43381 caacatatca tagaatcgta acggcatggc atcaacacca agctcccaca acaatacgcc
    43441 attacatcgc atcatcatac tacaactcca atgtctagtc gtgataattc cattccgtaa
    43501 aaaactgttt caagtacatc atcataaagt cttgacgttc tttagcaatc atcttcgccg
    43561 tatctgtatg cattaaagat tctaacttaa gtaatttttc aaagaaatgt ttaattgcag
    43621 atggtggcaa ctgttcaact aaatcatcat taatcttatc tagtgacata tgttctgtcc
    43681 acataggttc accaaagtgt cctgcaaatt gaaatgttcg tgcaacacct atagcgccta
    43741 tagcatcaag acgatctgca tccctgacaa tttgaccttc taaagataaa gtgacatgat
    43801 cgtttttacc attgcgatag ctcatattat taataataaa taaaatgtgc tcttgatctt
    43861 cggttgatag tgataaagaa gataaaaatg acttcaattc aacatattgt ttgttagcat
    43921 ctacaacttt ttcgtcaacg gtatcatgaa gcaaacatgc gagttcaatg actaaagtat
    43981 cattaacacc ttcattttca gcgattgatt tagctaaagc agtgacacga tatacatgcg
    44041 caatatcatg gcctgtataa tcattttgat gaatagattc catatatttt ttggctgcac
    44101 taatgatgtc tgattgttgc attaaagtta cactccccat atttctattt tataagatag
    44161 gtgacaataa ttgtgaaata ccttctttaa atttaatcca aagacttcgc ttagcataac
    44221 gttcttttgt taattcagaa gatactgcta aatcatctat aaaagcttgt tttaattttt
    44281 tggcaatttg ttggtcataa ataaaggcgt tgacttcgaa attcaatgtg aaactgcgat
    44341 ggtccatatt agctgttcca acacttgcaa tttcatcatc tataacaagt gtttttgagt
    44401 gtaaaaagcc attgtcgtag tgaaatactt taacaccggc atctaataag gatgctgcat
    44461 tttttaaagt agcccaaaaa acaaacggat ggtcaggttt attaggaatc atgatattga
    44521 catcaacacc acctaatgcc gcaattttaa tagaatctaa aaaggcttga tcaggtatga
    44581 aatagggaga ttgaatataa atcgattttt tagcagatga aatcattttc aaataaccgt
    44641 atttaatctg ttcccattct tcgtcaggac cactagaagc tatttgaacg ccaattgttc
    44701 caccagaatt tacatctggg aaataacgat catcatagga gatgtggtca cgtgtggctt
    44761 gtgaattcca atctagaata aatcgtaatt gcaatgcatt tactgcatcc ccgacaattc
    44821 gtaaatgcgt atctcgccaa tagccgaatt tttttgattt acctaagtac tcatcaccaa
    44881 cattaaaacc accaacatat ccaatttgcc catctataac aacaattttt cgatggtttc
    44941 gattgttcat acgcaagtta attaaaggta attttgatgg gaaaaatgct tcagcatgtc
    45001 cgcctttatt gcgaaacggg cgtaagcctt ttttacgcag tccacgagaa cccatgtcat
    45061 cataaagaat tttaacttct acaccttgtt ccgctttttt accaagttca tttaaaatgg
    45121 tacgacctaa ttcatcattt tgaataatat agtactgaaa atgaatataa tcagtagcat
    45181 tacggatgtc ttgtattaag tcatcaaatt tttcttggcc gtctgtgtat atttttaaat
    45241 cgttgtctgt tgttaaaaat gctgcgttat tatataacaa catttgaatc atttctttaa
    45301 attttacaat ttgataattg gaatttgaaa agttttcatt ttttaaagca gctaattgct
    45361 catcaacgat taactctaat ccttttttat cttccttatc aattttgaaa atttggtcac
    45421 gttgaatttg tcgtcctaat agtaagtata aaatgaagcc gaataaaggc aagaaaacta
    45481 agactagtaa ccaagcccag atagaattgg cagaacgtct ttccatgaaa ataatggtaa
    45541 aggcgaataa taagtttaaa ataaatgcac caataaatat tgaatttaaa ataatattag
    45601 aatgcttgag tgcaatggat aataactcta tcatttgaaa cctcccatcg aaaatcttaa
    45661 acgagtagta tatcttatta attgtatcat gaagcaattt aaatatcatg acaatttagt
    45721 ttgaaaaaca cactttgtgt tgacgttgtg ttttatggga tatagtatag taaggaatgt
    45781 aaatgaagga gtgaatgcta tgactgaaca agatattgca catcattctg aacaaataaa
    45841 aacgaatctt aaatcacgtt taaatcgaat tgaaggacaa gtgagagcga ttaatcgcat
    45901 gattgaagaa gatgtctatt gtgatgatgt ccttacgcaa ataagagcga cacgttcggc
    45961 gttaaacagt gttgcgacaa agttattaga acaacatatg aaaagttgta ttatgaataa
    46021 agttaatcaa ggtgctcagg aagaggcaat ggaagagtta ttagtgactt ttcaaaaatt
    46081 gattaaagac taaaggagtt taagatgata catcaaaata cgatttacac agcgggaatt
    46141 gaaacagaag aacaagtaag tcaattgaca gaacgcattt caaatatgat aggtgttcat
    46201 caagtgaata ttaatataat agacggtcaa gtaactgtat cgtatgagac accagcaaat
    46261 ttgaatagta ttgaaaaaga aatctatgat gaaggataca aaattgtatt ttagggtata
    46321 atgtagagtg cgctatagat tttaattttg aaaataaatt aaaaattttg taaatgatgt
    46381 agtaaaggta tgtcgaatag ggtaagataa tattgaaagc gatccaaacg aggtgataat
    46441 aatgaaacgt cctgaaaaga ttcaaaatgt agtcaaacta ttgtcatcat taggtgtgaa
    46501 tattaaaaaa actaaatctc gtttagacat tattaatact ttgccagcat ctaataaagt
    46561 aagtcacgaa ttaaaataat tgactgtgat tgtgaatcac acaccgttaa agctatcgtc
    46621 ggactattcg atgatagctt ttttgactgg atttactaaa atgatagcaa gtactatata
    46681 acaaaatccc tctttagagt gtacatttta caatgtgtac ttcaaagagg gattgttatt
    46741 atcgagtaat atctatatat gacattttaa atcgacgaca taaatagact atataacgtc
    46801 atcttgaaaa gttatgatat ttgaaactca aaatagttta aattaattac tttttggatg
    46861 gtataaatct acatatcgtt ttggttcatg ataataaatt gataagacta tacctattcc
    46921 agtcatcata ctccatagcg cactaccacc ataactaata aatggtaatg gaataccagt
    46981 gattggtaac aactgaattg tcataccaat attttgtaaa atatggaaca caagtaaagt
    47041 gacgaaacca acgataaaga ttttgttaaa ttgatcttca attttcgcag ctaatcttat
    47101 tagatggaag attaaaaata aaaatattaa gatcaatatg acagaaccga taaaaccaag
    47161 ttcctctcca atcactgaaa agataaagtc agtatgattt tcaggtatat aaacttcacc
    47221 gtgattgtat cctttaccta gtaactgtcc agaaccgata actttaagtg attcagttaa
    47281 atgatagcca tcaccactac tatatgtata ggggtcaagc catgaattga ttcgtcccat
    47341 ttgatacagt tggacaccta ataaattttc aattaatgaa ggtgcatata gaatacctaa
    47401 aatgactgtc attgcaccaa caatacctgt aataaagata ggtgctaaga tacgccatgt
    47461 tattccactt actaacatca cacctgcaat aatagcagct aatactaatg tagttcctag
    47521 gtcattttgc agtaatatta aaatacttgg tactaacgag acaccaataa ttttgaaaaa
    47581 taataacaaa tcactttgga atgatttatt gaatgtgaat tgattatgtc tagaaacgac
    47641 acgcgctaat gctaaaatta aaataatttt catgaattca gatggctgaa tactgatagg
    47701 gccaaacgtg taccaacttt tggcaccatt gataataggt gtaataggtg actcaggaat
    47761 aacgagcaag cctattaata atagacagat taagaaatac aataaatatg tataatgttt
    47821 aatcttttta ggtgaaataa acatgatgat acctgcaaaa attgcaccta aaatgtaata
    47881 aaaaatttgt ctgataccga aattagcact gtattgacca ccgcccattg ccgagttaat
    47941 aagcagaaca ctgaaaattg ctaaaacagc tatagtggct actaataccc agtctacttt
    48001 gcgaagccaa tgcttatccg gctgttgacg agatgaataa ttcattgcaa actcctttta
    48061 tactcactaa tgtttatatc aattttacat gactttttaa aaattagcta gaatatcaca
    48121 gtgatatcag ctatagattt caatttgaat taggaataaa atagaaggga atattgttct
    48181 gattataaat gaatcaacat agatacagac acataacgcc tcgtttttaa aatgcaaaat
    48241 agtattaaaa tgtgatacta ttaagattca aagatgcgaa taaatcaatt aacaatagga
    48301 ctaaatcaat attaatttat attaaggtag caaaccctga tatatcattg gaggaaaacg
    48361 aaatgacaaa agaaaatatt tgtatcgttt ttggagggaa aagtgcagaa cacgaagtat
    48421 cgattctgac agcacaaaat gtattaaatg caatagataa agacaaatat catgttgata
    48481 tcatttatat taccaatgat ggtgattgga gaaagcaaaa taatattaca gctgaaatta
    48541 aatctactga tgagcttcat ttagaaaatg gagaggcgct tgagatttca cagctattga
    48601 aagaaagtag ttcaggacaa ccatacgatg cagtattccc attattacat ggtcctaatg
    48661 gtgaagatgg cacgattcaa ggactttttg aagttttgga tgtaccatat gtaggaaatg
    48721 gtgtattgtc agctgcaagt tctatggaca aacttgtaat gaaacaatta tttgaacatc
    48781 gagggttacc acagttacct tatattagtt tcttacgttc tgaatatgaa aaatatgaac
    48841 ataacatttt aaaattagta aatgataaat taaattaccc agtctttgtt aaacctgcta
    48901 acttagggtc aagtgtaggt atcagtaaat gtaataatga agcggaactt aaagaaggta
    48961 ttaaagaagc attccaattt gaccgtaagc ttgttattga acaaggcgtt aacgcacgtg
    49021 aaattgaagt agcagtttta ggaaatgact atcctgaagc gacatggcca ggtgaagtcg
    49081 taaaagatgt cgcgttttac gattacaaat caaaatataa agatggtaag gttcaattac
    49141 aaattccagc tgacttagac gaagatgttc aattaacgct tagaaatatg gcattagagg
    49201 cattcaaagc gacagattgt tctggtttag tccgtgctga tttctttgta acagaagaca
    49261 accaaatata tattaatgaa acaaatgcaa tgcctggatt tacggctttc agtatgtatc
    49321 caaaattatg ggaaaatatg ggcttatctt attcagaatt gattacaaaa cttatcgagc
    49381 ttgctaaaga acgtcaccag gataaacaga aaaataaata caaaattgac taactgaggt
    49441 tgttattatg attaatgtta cattaaagca aattcaatca tggattcctt gtgaaattga
    49501 agatcaattt ttaaatcaag agataaatgg agtcacaatt gattcacgag caatttctaa
    49561 aaatatgtta tttataccat ttaaaggtga aaatgttgac ggtcatcgct ttgtctctaa
    49621 agcattacaa gatggtgctg gggctgcttt ttatcaaaga gggacaccta tagatgaaaa
    49681 tgtaagcggg cctattatat gggttgaaga cacattaacg gcattacaac aattggcaca
    49741 agcttacttg agacatgtaa accctaaagt aattgccgtc acagggtcta atggtaaaac
    49801 aacgactaaa gatatgattg aaagtgtatt gcataccgaa tttaaagtta agaaaacgca
    49861 aggtaattac aataatgaaa ttggtttacc tttaactatt ttggaattag ataatgatac
    49921 tgaaatatca atattagaga tggggatgtc aggtttccat gaaattgaat ttctgtcaaa
    49981 ccttgctcaa ccagatattg cagttataac taatattggt gagtcacata tgcaagattt
    50041 aggttcgcgc gaggggattg ctaaagctaa atctgaaatt acaataggtc taaaagataa
    50101 tggtacgttt atatatgatg gcgatgaacc attattgaaa ccgcatgtta aagaagttga
    50161 aaatgtaaaa tgtattagta ttggtgttgc tgctgataat gcattagttt gttctgttga
    50221 tgatagagat actacaggta tttcatttac gattaataat aaagaacatt acgatctgcc
    50281 aatattagga aagcataata tgaaaaatgc gacgattgcc attgcggttg gtcatgaatt
    50341 aggtttgaca tataacacaa tctatcaaaa tttaaaaaat gtcagcttaa ctggtatgcg
    50401 tatggagcaa catacattag aaaatgatat cactgtgata aatgatgcct ataatgcaag
    50461 tcctacaagt atgagagcag ctattgatac actgagtact ttgacagggc gtcgcattct
    50521 aattttagga gatgtcttag aattaggtga aaatagcaaa gaaatgcata tcagtgtagg
    50581 taattattta gaagaaaagc atatagatgt gttgtatacg tttggtaatg aagcgaagta
    50641 tatttatgat tcgggccagc aacatgtcga aaaagcacaa cacttcaatt ctaaagacga
    50701 tatgatagaa gttttaacaa gcgatttaaa agcgcatgac cgtgtattag ttaaaggctc
    50761 acgtggtatg aagttagaag aagttgtaaa tgctttaatt tcatagaaat tagtcgagcg
    50821 accttttact tagaaaatga tttgaattaa tactaaaaga ttacaaagaa gaggtggttt
    50881 tgtgtgtaaa tacaaaattg cctttttctt tttatgttaa atctataaat ttgaaactaa
    50941 atcaaggtta attctatgta cacactttat ataggaagta gtttgaatgt ttatataata
    51001 ttttacaaaa agatgttgta ttataaggtc taatttcaca tgtgtttcag taaaatttgt
    51061 tgtgaaatgt taacgatata cgtattttat aaaaaatttt ttataatgat tattcgaatg
    51121 atgcataacg cttacatctt atctaatgct agctttttga caaaaatatg acaatcaatt
    51181 aatgtgattc taataaatat tcgcaaattg ctttattgcg attaaatttt tttggtggta
    51241 ctatatagaa gttgatgaaa tattaatgaa cttatatgca aaagtatatt gagaaataaa
    51301 caggtaaaaa ggagaattat tttgcaaaat tttaaagaac tagggatttc ggataatacg
    51361 gttcagtcac ttgaatcaat gggatttaaa gagccgacac ctatccaaaa agacagtatc
    51421 ccttatgcgt tacaaggaat tgatatcctt gggcaagctc aaaccggtac aggtaaaaca
    51481 ggagcattcg gtattccttt aattgagaaa gtagtaggga aacaaggggt tcaatcgttg
    51541 attttagcac ctacaagaga attggcaatg caggtagctg aacaattaag agaatttagc
    51601 cgtggacaag gtgtccaagt tgttactgta ttcggtggta tgcctatcga acgccaaatt
    51661 aaagccttga aaaaaggccc acaaatcgta gtcggaacac ctgggcgtgt tatcgaccat
    51721 ttaaatcgtc gcacattaaa aacggacgga attcatactt tgattttaga tgaagctgat
    51781 gaaatgatga atatgggatt catcgatgat atgagattta ttatggataa aattccagca
    51841 gtacaacgtc aaacaatgtt gttctcagct acaatgccta aagcaatcca agctttagta
    51901 caacaattta tgaaatcacc aaaaatcatt aagacaatga ataatgaaat gtctgatcca
    51961 caaatcgaag aattctatac aattgttaaa gaattagaga aatttgatac atttacaaat
    52021 ttcctagatg ttcatcaacc tgaattagca atcgtattcg gacgtacaaa acgtcgtgtt
    52081 gatgaattaa caagtgcttt gatttctaaa ggatataaag ctgaaggctt acatggtgat
    52141 attacacaag cgaaacgttt agaagtatta aagaaattta aaaatgacca aattaatatt
    52201 ttagtcgcta ctgatgtagc agcaagagga ctagatattt ctggtgtgag tcatgtttat
    52261 aactttgata tacctcaaga tactgaaagc tatacacacc gtattggtcg tacgggtcgt
    52321 gctggtaaag aaggtatcgc tgtaacgttt gttaatccaa tcgaaatgga ttatatcaga
    52381 caaattgaag atgctaatgg tagaaaaatg agcgcacttc gtccacctca tcgtaaagaa
    52441 gtacttcaag cacgtgaaga tgacatcaaa gaaaaagttg aaaactggat gtctaaagag
    52501 tcagaatcac gcttgaaacg catttctaca gagttgttaa atgaatataa cgatgttgat
    52561 ttagttgctg cacttttaca agagttagta gaagcgaacg atgaagttga agttcaatta
    52621 acttttgaaa aaccattatc tcgcaaaggc cgtaatggta aaccaagcgg ttctcgtaac
    52681 agaaatagta agcgtggtaa tcctaaattt gacagtaaga gtaaacgttc aaaaggatac
    52741 tcaagtaaga agaaaagtac aaaaaaattc gaccgtaaag agaagagcag cggtggaagc
    52801 agacctatga aaggtcgcac atttgctgac catcaaaaat aatttataga ttaagagctt
    52861 aaagatgtaa tgtcttgagc tcttttttgt tttcaataat tgattctctg tagatatcaa
    52921 agtactaacg ttttaaaggt taaatatttg attggattga gatctgtgtg cggttatatc
    52981 attctgttta aatatggttc tccaccaaat gtggtgggta tataatttaa agaactattt
    53041 ttaaattaag aataatcgaa cataaataaa ctttatgaaa tttcagtatc atgttcttat
    53101 aaaaaacaat agagcatttt gctgacacta gtgcgcgata aataataagt tgaatataaa
    53161 aaagatcact gccaatcatt cgtttaatgg cagcgatctt ttttatttaa ttatttctct
    53221 ttccactgca acatttgata accaatgcgt ggatgtgttt taataatatc ttttgcgtcc
    53281 tcatgacatt gtgaaagttt ttgtcttaat gatgtcatat gcactcttaa actaggcatt
    53341 tcagtcttat taacatagcc atatattcgt tttaatatca tctcataagt gagtactttt
    53401 cctttatgat ttgacaatag ttctaacaag ctaaattcat ttggtgtcaa atgtacctcc
    53461 tgattattaa taacaacaga tttggagcca aagtcgatgc ttagcaaacc gttagtaaat
    53521 acaatgttag tttcttgatg tgacttagcg attctctcga tgactcgtat tcgtgcccga
    53581 agctcatcaa cattaaaagg tttagtcata tagtcattcg caccgttatc taaagcttga
    53641 ataattgttt gttcttcttg tcttgcactt attacaatga taggaatgtc agtatgttgc
    53701 ctgatttctg aaatcaaaca taatccatct ttatctggta aacctaaatc taataaaatg
    53761 acatctggtt tatcaatttg aattttaaag tgtgcttgtg tggcattgtc ggctgtagtt
    53821 acattgtaat aatctaaagt taatgcaaca tcaagtaaat gtgtgattgc gtgatcatct
    53881 tcaattatca atattttaga ttgcattata tgtctccttc attaaagtct gtatctatat
    53941 tgaaatagaa tatactgccg tgtggttggt tcggtttata ttgtaagttt gattgatgtt
    54001 tgtgtaggat agtctgtact aaatataagc ctagtcccat gctttctttt tggttatctt
    54061 taaaatattt atttgatcct gtgtaaaaag gctcgaatat cttttgttgt tcttctaaac
    54121 taattccagg tccttcgtct ataacggcaa attcgatttg ttcatcgcta gcataacgaa
    54181 tagataaatt gattttggtg tcagtagaag tgtgtttaac tgcattttca atcaaattga
    54241 ataaagcttg taaaatcaac ttactgtcaa tgtgtataaa ctgtaaatct actgaggatg
    54301 atacagttat acgctttttt aaatggcgac gttctagaat catatcgatt tcttctacta
    54361 attcactaac gagataaggt tgcaatttta tctgaacatt tgatgactgt aattttgtta
    54421 atgataaaat atttgtcact aatagatata aatactgact ttcttgaaaa ctatgcacaa
    54481 gtaattgttc tttttctatg atagacatat ctttactatg tgatactaaa atatctaaat
    54541 ttcccataat tgttgttaac ggtgtacgta tgtcatgcga aattgatctt aaaaaatttg
    54601 aatgtgtcag ttgacgttca gcctgtaaca tggattctct cgtttgttta agtaacgaca
    54661 cattttcaac ggcgagagaa agttcattta acattgattc taatattgat gcatcatatg
    54721 gattaatcac ttgagaactt tggtaatcaa tggctagaat gcctttaatc ggagatgtgc
    54781 caattggtat caaccattta ttaatgcctg gaaatgtatc tgttgttgca ccagcttgtc
    54841 tttcattttt aattacccag cttaatgctt gttcatgctg ttgagtcgta ttatcgatat
    54901 ggttttgcaa tggtattgtt ttaattactt tcgattgatt gataacgtat acagtaattg
    54961 attgttgcaa taattgatta atttggtatc cagcatttat tagtaagttt tcaactgtat
    55021 aagtttgttt aatcgaatca ttaaattgaa ataataaatc tgtacgataa agttgctttt
    55081 tagtaatgga gtattggaat ttaatttgtt ttaataaagc actcgttaaa atacttgtta
    55141 aaatgctaac gataaatgta ataggatagt caaagcggta tacttcaaat gtatatctag
    55201 gttccgtaaa aaaataatta aaaacaaata cgttaataat tgctgctaaa aagccaatga
    55261 tgtaggaccg cgtccaaatg gataataaaa tgatgccgat gagaaaaatc attaaaataa
    55321 tcgtactaga ctcatgctta tcaagttgat aaatccacag tcccatcatt acacaaatta
    55381 tttgaatcaa aatcattttt aacatatcta tggcgaaacg tttgccttta ggacgatagg
    55441 gtgatttgtt cattttcaat tcaacaggta tttgtttgat tggaacgatt tcgattttat
    55501 agctatgttc aaaggacatt aaatggtcaa ttaaaggtgt attgaaaaag tctcgccact
    55561 tatttctaat atgttgtccg ataattaatt tggttacatc ttgattttta caccattcgt
    55621 ctaatcctaa tgcaacggtt tggctataaa ctactttcac ttttgctcct aaagattttg
    55681 caagcatgag atgttgatgc acttgctttt gactatcttt atattgcctg ttcttttcga
    55741 atacatctat ataaatagca gtgaattttg catgttcttt ttgagcaata tggaatgcct
    55801 ctttaattac tgcttcatta taaatgctcc cactaattgc cacagcaata tgaggtttga
    55861 gtgacgtttt atgattgtgt cggacttttt ctttatcact catcaagtcg gcaactgttc
    55921 ttaacgtcaa tgtacgcagt tcgcttaggt gggtatacgt aaagaaatta ctaaatgcta
    55981 catctagcct atccttttta tataccttgc cagcttttaa gcgcttaatt aattgttcag
    56041 gtgagatatc tacgacttct aatacatcgg cgctcattat gaaatagtcg ggtacacgtt
    56101 ctttaacatg tacaccggtc atcagttcaa tttgactact taaactttcg atatgttgaa
    56161 tgttcaaagt agtatgaaca tcgataccat gatttaaaat ttcttcaata tccatatatc
    56221 gtttctcatg acgatctcta gaaatgttcg tatgtgctaa ctcatcgata agtactattg
    56281 tcggcgattc tttgattatg cggtcgacat ctaaatattg aaaatgatga ctaccatgtt
    56341 tagtaaaatt ggttgcgatt ttaggtaatt gttcagctaa tgcatttgtt tcatcacgtt
    56401 gatgcggttc aatatatcct attttaatat caacgttact ttgaaatagt tcaatggcat
    56461 ttgaaagcat ctcaaatgtt ttaccaacac ctggactgta accgatgtaa attgttaaag
    56521 atccacgctt ttttcctatg tttagcgatt cagtgtttga cataaccttc acctcgatag
    56581 caatatcata atattcttca caattaaatg ctataaaatt tatttctgca tacacatctt
    56641 aatgatttct taataactaa gtttgaaaat taatatttct ctcataaaca agttgttacg
    56701 atgttgttgt gttgtaagga ggttatggca atgctcatcg ttttattatt ggtagttatt
    56761 gcattagttt tgtatttatt ttatgcctta attcgtagtg aaaaatttta gaaatatatt
    56821 aagtgagggt tatcatggaa ataattttgt ttctaacaat gatggttatg attacgtatg
    56881 tttttagtgg atatttatat cgcgtagcat tggtgcaatc gtcaagagta gatttaatat
    56941 ttactagatt tgaaaatatg tgttttaaaa ttatcggcac agatttagaa cacatgtcag
    57001 ctaaaacata tgtcaaacat tttctggcat ttaatggatt tatggggggt atatcatttg
    57061 tattgctaat agttcagcaa tggctttttt taaatccaaa tcataattta aaccaatcga
    57121 tagatttagc gtttaataca gcaatatctt ttttaacaaa tagtaattta caacactata
    57181 acggtgaatc aggtgtgaca tatttaacgc aaatgattgt aatgacatat ttaatgttta
    57241 catctagtgc atcaggttac gccgtttgta taacgatgtt gagacgttta actggattga
    57301 ctaatatcat tggtaatttt tatcaagata ttgttcggtt tattgtccga gtacttttac
    57361 cattatcatg tttaattagt attttactga tgactcaagg tgtaccacaa acgttgcatg
    57421 ctaatttaat gattaggact ttaagcggac atattcaaca tattgcattt ggacctattg
    57481 catcacttga atcaataaaa catcttggta cgaatggtgg aggattttta gcaggaaatt
    57541 ctgcaacacc ttttgaaaat ccaaatattt ggagcaattt tatagaaatg ggcagtatga
    57601 tgttacttcc tatgtcaatg ttgttcttat ttggtcgcat gttaagtaga catggtaaac
    57661 gagtacatcg tcatgcgctg atattatttg ttgcaatgtt tttcattttt atagcaattc
    57721 ttacattaac tatgtggagt gagtatcgtg gtaatccaat actagcgaat ttaggcattt
    57781 atggaccgaa tatggaaggt aaagaggtac ggtttggagc aggcttgtca gcactattta
    57841 cagttattac gacggcattt acaacgggtt ctgttaataa catgcatgat agcttaacgc
    57901 ctctaggtgg attaggacca atggtattaa tgatgctaaa tgttgtattt ggtggcgaag
    57961 gcgtaggact catgaattta ttgatatatg tcttactgac ggtgtttata tgcagtttga
    58021 tggttggtaa aacaccagaa tatttaaata tgccaattgg cgcccgtgaa atgaaatgta
    58081 ttgtcttagt ctttctcata catccaattt taagtttagt attttcagca cttgctttta
    58141 tgattcctgg agcaagtgaa agtataacga atccgtcttt tcatggtatc tcacaagtta
    58201 tgtatgaaat gacgtcagct gcagcgaaca atggatcagg gtttgaagga ctgaaagatg
    58261 atacaacatt ctggaatatc tctacaggaa tcattatgtt gctttctcgt tatataccaa
    58321 ttattttgca attaatgatt gcatcaagtt tagtgaataa aaaatcatac caccaagata
    58381 aatatacaat agcgattgat aaaccttatt ttggcgtatc actaatcgta tttatcgttt
    58441 tactaagtgg tttgacattt attccagtac tattacttgg tcctattggt gaatttttaa
    58501 ctttaaaata aagtaaaggg atggaaaaaa tgcatcatgt aaataaatat ttcaatcaaa
    58561 caatggtgat agaagctttg aaaatgagtt tttataagct gaatcctaaa caattaataa
    58621 aaaatccgat aatgtttgtc gttgaggttg gaatgatttt aacgttgata ttaatatgtt
    58681 ttcctgacat atttggaact agctatctat cgcgaggtta tttgattaca atttttatca
    58741 tattattaat aactatattg tttgctaatt tttcagaagc ctttgctgaa ggtcgtggaa
    58801 aagcgcaagc tgatagttta agacaagcac aatcaaattt aactgctcga ttgatagaag
    58861 aaaatggtgc gtatcggata gtgaacgcca cagagttaaa agccggacaa aatattcggg
    58921 tagaaaatgg agaaacaatc cctgctgatg gcgttgtaat aaatggatta gcaaccgttg
    58981 atgaatctgc aattactggt gagtctgcac ctgtgataaa agaatcaggt ggcgattttg
    59041 atggagtcat tggcggtaca cttgtaacat ctgattggtt agaaatacgt gttgagagtg
    59101 aagcgggtac atctttttta gataaaatga ttgccttagt tgaaggggcg gaaagaaata
    59161 aaacaccaaa tgaaattgca ctttttacat tattaacaac gttaacaatt atatttttag
    59221 tcgttattga aacgttatat ccaatagcat cttacctgca tttaatttta cctattgcga
    59281 tgctcattgc gttaacagta tgtttaatac caacgacaat cggtggtttg ttatcggcga
    59341 ttggtattgc aggcatggat cgtgttactc aattcaatgt attagcgaaa agtgggcgtg
    59401 ctgtagaagt atgtggagat gtcgatgtaa tgattttaga caagacaggg acaatcactt
    59461 atggcaatcg aatcgctagt gaatttttac cagtgaatca acaaatgctg gaaaaattga
    59521 tagtcgctgc ttatatgtca tcaatctacg atgatacgcc agaaggaaaa agtattgtac
    59581 gattagcgaa gcagatgtat ataaatgagc tgcctaaaga cattgatggc acatataagc
    59641 cttttacagc tgaaactaga atgagcggaa tcattacaaa tgaaatatcg gtattcaaag
    59701 gtgcacctaa tagtatgata aatcttgtca aacaacaaca aggcaatatt cctcttaata
    59761 ttgagtctct ctgtatggat gtatcgagta aaggtggcac accattaata gtcattgaaa
    59821 acaatgtcat gcttggcgtg atttatttaa aagatgtgat taaagatggt cttgtagaac
    59881 gatttactga attgagaaaa atggggattg aaacagttat gtgtacgggt gataatgctt
    59941 taacagcagc tacaattgca aaagaagcgg gtgttgatag atttgttgca gagtgtaaac
    60001 ccgaggataa aattaaagtc atcaaagatg aacaagcgaa aggccatatt gttgcgatga
    60061 caggagatgg tacaaatgat gcacctgcgc tagcacaagc gaatatagga ttagctatga
    60121 actcagggac aattagtgct aaagaggcgg ccaatctaat tgatttagat tcgaatccta
    60181 caaagttaat agaggttgta aaaataggca aacaattatt aatgacgaga ggtgcattaa
    60241 cgacatttag tttagctaat gatgtagcaa aatactttgc aattttacct gcactaatga
    60301 tgtcaacgat tccagaaatg acttcactaa atattatgca tttatcgtca cctaaatctg
    60361 caatcatatc agctttgata ttcaatgcat taatcattgt tgccttaatt ccaattgcca
    60421 tgaaaggtgt aaaagtaaag gggtacagca ttgatagaat ttttataaat aatatgttga
    60481 tttatggatt aggtggatta atcgtcccgt ttttaggcat taaattaata gatatgattg
    60541 tacaattttt tgtgtaaaga ggaaggagcc tgtaccatga atacgataag aaatagtatt
    60601 tgtttaacga taataactat agtattatgt ggatttttat tcccgttagc tatcacgtta
    60661 attggtcaaa tattttttta tcaacaagca aatggtagtt taattacgta tgacaatcgt
    60721 atagttggat caaaattgat tggacagcac tggacagaga gacgatattt ttacgggaga
    60781 ccgagtgcag ttgattataa tatgaatcct gaaaaattat ataaaaatgg cgtatcatca
    60841 ggtggaagta atgaatcgaa tggaaacaac gaattaatag cacgtgtgaa acatcatgtt
    60901 aaatttggta attcaaatgt aacaatagat gctgcaacaa gttctgggtc gggcttagat
    60961 ccgcatatta cggttgaaaa tgcattgaag caagcacctc gtattgctga tgttcgtcat
    61021 atatctactt caagagtggc cgacttgatt caacatcgta agcagcgtgg tgtgttaaca
    61081 aatgattatg tgaatgtgct tgaactaaat attgcacttg ataatatgaa agattgaata
    61141 tagatgaaag gcatcatgtt gataaaatta taacatggtg tctttttagc aattcaagca
    61201 ctttctgctt aactaaattt ttagatgtac ctttaggaag aataaattag catgaaatat
    61261 tccatatgct ataattatca acatataaaa ataaaatgaa atagattata aatccgtata
    61321 attttttatt tggggagagg ggtacatgtc agattatact aagttgtcta agagtcctaa
    61381 gtcagcattg ttgtattact acattacgaa tggcttagaa tttatattga gtgtagctgt
    61441 gtatgtcatt ttttatttta tatggttaag atttgaatgg ccacattact tactttatat
    61501 tttgttggtt ttaggtgctc tgagtacatt aaaattattt attaagcctt tatggcaata
    61561 tcattgtcgc ttttatcaag ttgatcaact aagtgtacaa tatcgtacat catttttaat
    61621 atacaaagaa gaaacgagtc gaattgaacg tttacaatac ttgtcgatta aatctaatcc
    61681 aattagtaaa gtgcttaatt tgtataaagt tggatttatg actgcaggtc atactattaa
    61741 tttaccgatg atgtcacatg acgatgttaa aataatcgaa gcgagagcta tgtcgaattt
    61801 gagaggggtt gaaagtgatg tctgaacctc aaaaattaca tccgatttca tattttaatg
    61861 gcataattaa tgctatcaaa caaaacatag tcgtcttttt tattttcatt atctttcaat
    61921 tgaaggattt tgattatacg aatcccgaat catatttatg gataggtatt gtatttgtat
    61981 tctttttaat atcatacatt tctcaaattg tcggaattat gaacactcgt tattggattg
    62041 aggataatta tttcatttta acaacaggta tttttaataa aaagcgtaaa gaattaaata
    62101 taaaacgtat tcagtccgtt gatatgacac aaggggtcgt caatcaaatt attggtggtg
    62161 tggatttaca aatcaaaaca ccaagtgatg gtattgtctt aagtgttatt tcgaaaaaac
    62221 aaggtgaata tcttgaacga tatattgatc aattgcaaac agagttgaag acagaagggg
    62281 cgcaaaagtc tagtgctgag aagaatgaca actgtaatca tgacattgaa ggtaatgtgg
    62341 aagatatcgc agaaggtcgc ttagatgaag tgcagaacac tgatagtatg cttaatcaga
    62401 aaaataaacg cattcctata tatcagatga attttaaaga atcactattt atggcaatga
    62461 ccagtggcgc cattggtgta acgttagcag ctttagtacc gatttacggc gctgtaagag
    62521 agttgatccc gtggtcgaag ctaaatcatg agattagtca atgggttcaa attgtaagta
    62581 tttcagtatt aatcatcatt ttactagtat taatagtggc atatattatt ggaacgatta
    62641 taacgatgat aagattttat ggcttttcgg tgatgcttga aaatgagcaa cttaaaattg
    62701 aatatggtct ctttaacaag aagaaagtaa ctgtaccaac gagacgattg caagctgttg
    62761 ttgaacgtca gtcatatata cgaagaatat ttggctacac agcaatacat tttatcatca
    62821 caagtgattt tgaaaataaa aaaatcgaag atgatgacaa tgattcaggt agtgtgacga
    62881 tattgccgtt tattaaacga gacaaagcat atcaaattat ccagaattta gtaccagatt
    62941 tatcttatca acaagttaat gaaggtatgc ctttttcagg ttttcacaga cactttttaa
    63001 tgcctagtat tattgtcata gctatggcag gtattggctg ttatttttgg aatcaattat
    63061 tattgttatt tacgattata acaatagtca ttattggttt atttgtgatc aaaggatatc
    63121 tttatgtcaa acatgcgggt tttaaaatga cagaagaaca aattactatc cagaatttcg
    63181 agctattgaa acgaaatatt tattatttta aagcgcatca cattatagga tttgacactt
    63241 ggcaacatac attacttaaa cgcaacaatt taaataattt tagctttatt gttgctaaag
    63301 gtgtgggagc ccaatcgatt aacttaaaat acgcaagaag tgaagatgca aataaattga
    63361 aatcatggta tttgagaggt gaaaatgatg agggattata attttatgtc accacacgct
    63421 aaaaaagtga tgaggttaag tgctgtgatg ttttggatac caatagcgct tgtactttta
    63481 atcgcattta atattttgaa ctggctattt tgggagatat tagataacta tatatccatt
    63541 gttagtagtg tgattataat attacttgtt gctgttttca cgcttgttat agcgccagtc
    63601 tatcgatata agcactgcag atatgcattt gaaaaacacc atttgcgtgt acgaaacggc
    63661 attttatttt tggatgaaaa agtaatacca tattttcgta ttcaaaatat tgatatcgat
    63721 gttggtccta taatgagacg ctatcgatta gcaactttga cactttttac agcgggtggt
    63781 caagctgaaa ttgaattaat agatagaagt gaagcacgta gattgaaacg gtggttaaat
    63841 aacgagagac atttagaaaa caataaaaat gaggaataat caatgataca tggaattggt
    63901 gtagatttaa tcgaaatcga tcgaataaaa gcgttatata gtaagcaacc aaaattggtt
    63961 gagcggattt taacaaaaaa tgaacagcac aaattcaaca atttcacaca tgagcaacgt
    64021 aaaattgaat ttttagctgg caggtttgct acaaaagaag cgttcagtaa agcattaggc
    64081 acaggcttag gaaaacatgt agcttttaac gatatagact gttacaacga cgaacttggc
    64141 aaaccaaaga ttgattacga agggtttatc gtacatgtta gtatctcaca cactgagcat
    64201 tatgcgatga gccaagttgt tttagaaaag tcagcatttt aaatttgtta tacaatgata
    64261 attaagattt agttttaaaa cgtgtgggaa ttacaggagg aagtaatatg tcggataaat
    64321 attatagatc tgcgtatatg aatgtagatt taaacgctgt tgcatcaaat ttcaaagtat
    64381 tcagtacatt gcatccaaat aaaacagtga tggctgtcgt taaagccaat gcctatggac
    64441 taggtagtgt taaagtagca cgtcatttaa tggaaaatgg cgccacattt tttgctgtag
    64501 caacgttaga tgaagcgata gaacttagaa tgcatgggat tactgctaaa attttagtct
    64561 taggtgtgtt accagctaaa gatattgata aagcgataca acaccgagtt gccttaacgg
    64621 ttccgtctaa acagtggttg aaagaagcaa ttaaaaacat ttctggtgag caagagaaaa
    64681 agttatggtt gcatattaaa ttagatacag gaatgggacg tttaggtatt aaagatacta
    64741 aaacgtatca agaagtgatt gaaatcattc aacaatatga gcaacttgta tttgaaggcg
    64801 tgtttacaca ctttgcctgt gctgacgaac caggagatat gacaactgaa caatatcaac
    64861 gttttaaaga tatggtcaat gaagcaatta aacctgaata tatacattgt cagaactcag
    64921 caggttctct attaatggat tgccaattct gtaatgcaat aagaccagga atttcccttt
    64981 atggatatta tccatcagag tatgtacagc aaaaagttaa agtacacctt aaaccaagtg
    65041 tgcaattaat tgctaatgta gttcaaacaa agacgctaca agcgggtgag tctgtaagtt
    65101 atggtgcaac ttatacagct actgatccaa caacaatagc attgttacct attggatatg
    65161 cagatggcta tttacgcatt atgcaaggta gctttgtaaa tgtaaatggt catcaatgcg
    65221 aaattattgg tcgcgtatgt atggatcaga caattgtaaa agtgccaaat caagttaaag
    65281 ctggagattc ggtgatttta atagataatc atagagaaag tccacagtcg gtagaggtgg
    65341 cagctgagaa gcaacatact attaattatg aagtgctttg taacttgtcg agacgtttgc
    65401 cgcgaatcta tcatgatggt gatcaacgtt ttgtaacaaa tgaattgtta aaataatatg
    65461 gtcagtgcat ataaaatttg ttatcattag agtaattaaa ggtcatttaa taacttttgg
    65521 aatcaattgg aggttctcat atgttatctt ttagtcaaaa tagaagtcat agcttagaac
    65581 aatctttaaa agaaggatat tcacaaatgg ctgatttaaa tctctcccta gcgaacgaag
    65641 cttttccgat agagtgtgaa gcatgcgatt gcaacgaatc atatttatct tctaattcaa
    65701 cgaatgaatg attagacgag gagatgttta tttagcagat ttatcaccag tacagggatc
    65761 tgaacaaggg ggagtcagac ctgtagtcat tattcaaaat gacactggta ataaatatag
    65821 tcctacagtt attgttgcgg caataactgg taggattaat aaagcgaaaa taccgacaca
    65881 tgtagagatt gaaaagaaaa aatataaatt ggataaagac tcagtcatat tattagaaca
    65941 aattcgtaca cttgataaaa aacgattgaa agaaaaactg acgtatttat ccgatgataa
    66001 aatgaaagaa gtagataatg cactaatgat tagtttaggg ctgaatgcag tagctcatca
    66061 gaaaaattag gcgtctatta tatgtatttt tcagagataa ataaaatatt gatataaaag
    66121 acaataactt tataataatt ataactattt ctaaattctg tgcgaagaat ttcttataaa
    66181 ttaagatttt agcaaatacc agttatgata ttcatatttt ttattataaa aggatgtctt
    66241 aagtttttta ggctttaggt attccatcct aaagtttttt ttagcttaaa agtatcatct
    66301 acagcaaaat tgcaaacgac aaaattgata agtgcaatta aataaatgtt agtaagtgaa
    66361 tcataattat ccttgcttaa gcatttgctt tgtaagggaa gtgaggaggc aactaatcgt
    66421 ggaagaattt aagcaacatt ataagggttt aattgatgaa agtttaacgt gccaagataa
    66481 agtagaattg ataaaaaagt gtgagaaata cactgacgaa gtgattcgta aggacgtctt
    66541 gcctgaagac attgtcgata ttcacaaaaa ctatatattg acgttaaact taacgcgtga
    66601 agatgtgttc aagacattag atgtcttaca agaaatcgtt aaaggctttg gttatagtta
    66661 tcgagattat caaagattgg tagataaact tcaagttcac gataaagaga tagacttagc
    66721 ttctagctta caacaaacaa tgcttaaaac agatattcca caatttgata gtattcaaat
    66781 tggcgttatt tcagtggcag cacaaaaagt aagtggagat tattttaatt taattgacca
    66841 taacgatggc acaatgagct ttgctgttgc agatgtcatt ggaaaaggta taccagctgc
    66901 tttagcaatg agtatgataa agtttggtat ggattcttat ggacactcac aattaccgag
    66961 tgatggctta aaacgtttaa atcgtgttgt tgaaaagaat attaatcaaa atatgttcgt
    67021 cacaatgttt tatggtttat atgaagaaat gaaccattta ttgtattgta gttcagctgg
    67081 tcatgagcct ggatatattt atcgcgctga aaaagaagaa tttgaagaaa tttcagttag
    67141 aggtagagtg ttaggaatca gttcacaaac acgatatcaa caacaagaaa ttccaatata
    67201 ccttgatgat ttaattatca ttttaacgga tggtgtgact gaagctagaa atagtgaagg
    67261 tacctttata gataaacaaa aacttttaga atatattaaa aaacataaac atatgcaccc
    67321 acaagatatt gttcaaatta tctatgaagc aattttaaag cttcaaaacc caaataaaaa
    67381 agatgatatg actattttga ttataaaaag agtaaattaa ttttaaaaaa aaagattaga
    67441 aattatttcg atgggtatat aataatttga aatataaata tggtggatac agcgcttaaa
    67501 atgaagataa atatttttaa taagtaggag tgtaatgaaa tgaatcttaa tatagaaaca
    67561 accactcaag ataaatttta cgaagttaaa gtcggtggag aattagatgt ttatactgtg
    67621 cctgaattag aagaggtttt aacacctatg agacaagatg gaactcgtga tatttatgtt
    67681 aatttagaaa atgtgagtta tatggattcg acaggtttag gtttattcgt aggtacatta
    67741 aaagcattaa accaaaatga taaagaacta tacattttag gtgtgtcaga tcgtatcggt
    67801 agactatttg aaattactgg tcttaaggat ttaatgcatg ttaatgaagg aacggaggtc
    67861 gaataacatg caatctaaag aagattttat cgaaatgcgc gtgccagcat cggcagagta
    67921 tgtaagttta attcgtttaa cactttctgg cgttttttcg agagctggtg ctacatatga
    67981 tgatattgaa gatgccaaga ttgcagttag tgaagctgtg acaaatgcag ttaaacatgc
    68041 atacaaagaa aaaaacaatg tgggcattat taacatatat tttgaaattt tagaagataa
    68101 aattaaaatt gttatttctg ataaaggtga cagttttgat tatgaaacaa ctaaatcaaa
    68161 aataggtcca tacgataaag acgaaaatat agacttttta cgcgaaggtg gcctaggttt
    68221 atttttaatc gaatctttaa tggatgaagt gacagtatat aaagaatctg gtgtgacaat
    68281 cagtatgact aagtatataa aaaaagagca ggtgcgaaat aatggcgaaa gagtcgaaat
    68341 cagctaatga agtttcacct gagcaaatta accaatggat taaagaacac caagaaaata
    68401 agaatacaga tgcacaagat aagttagtta aacattacca aaaactaatt gagtcattgg
    68461 catataaata ttctaaagga caatcacatc acgaagattt agttcaagtt ggtatggttg
    68521 gtttaatagg tgccataaat agattcgata tgtcctttga acggaagttt gaagcctttt
    68581 tagtacctac tgtaatcggt gaaatcaaaa gatatctacg agataaaact tggagtgtac
    68641 atgttccgag acgtattaaa gaaattgggc caagaatcaa aaaagtgagc gatgaactaa
    68701 ccgctgaatt agagcgttca ccttctatca gtgaaatagc tgatcgatta gaagtttcag
    68761 aagaagaagt gttagaagca atggaaatgg gacaaagtta taatgcgtta agtgttgatc
    68821 attccattga agctgataaa gatggttcaa ctgttacgct attagatatt atggggcaac
    68881 aagatgacca ttatgactta actgaaaaac gtatgatttt agaaaaaata ttacctatat
    68941 tatctgatcg cgaacgagaa atcatacaat gtacgtttat tgaaggattg agtcaaaaag
    69001 agacaggtga gcgtatcggt ttaagtcaaa tgcatgtatc acgacttcag agaacggcaa
    69061 ttaagaaatt acaagaagca gcacataaat agaatttgct tattaatgat acgttttata
    69121 atgaaaaatc catataatta tcccttgatt attaaattga aatcgagggg tatttttaat
    69181 ttaattaagg ttttcgaatt aatgcgttat taacgtagtt taatgtgtat ccacataaat
    69241 gtcgcgatat agtattaata atttaagtga agaagatatc taattgtcgg tttaattaag
    69301 tggattgcta ttagaataaa aaaagtaggc ttagattatg aaatttagga aatgatggtg
    69361 tgtcattttc aataatctta gtgcatttta aaatgttgta tgacctaata attcattttt
    69421 aaagttttgg aagttaaaat tgcattaagt atcatacctt aatagaagta ttttagaata
    69481 tgttaaaata aatgagtaaa tttaagaaaa agtgtgggtt aagtaaatgg acaatcaatt
    69541 gattaattca atcatagaga aatatcaatt tagtaaaaaa caaattgaag cagtgttaac
    69601 actgctagaa gaaaaaaata cagtaccatt tattgcgagg tatcgaaaag agcaaactgg
    69661 tggactagat gaagttcaaa taaagcaaat tgatgacgaa taccaatata tggtcaattt
    69721 acaaaaacgt aaagaagaag ttatcaaaaa tatagaacag caaggattac ttactgagga
    69781 attaaagaac gatattttaa aacagaacaa attacaacgt gttgaagacc tatatagacc
    69841 ttttaaacaa aagaaaaaga caagggcaac tgaggcgaaa cgtaaagggt tagaaccatt
    69901 agcgatatgg atgaaggcac gtaaacatga agtctcaatt gaagaaaaag cacaacaatt
    69961 tataaatgaa gaagtgcaat cggttgaaga tgctatcaaa ggtgcacaag atattattgc
    70021 ggaacaaatt tcagataatc ctaaatatag aacaaaaatt ttaaaagata tgtatcatca
    70081 aggtgtgtta acttcatcta aaaagaaaaa tgctgaagat gaaaaaggta tttttgaaat
    70141 gtactataaa tatagtgagc caattaaacg cattgctaac catagagttt tagctgttaa
    70201 tcgtggtgaa aaagagaaag tattatctgt aaagtttgaa ttcgatacga caacagtaga
    70261 ggatttcatt gcacgtcaag aaatcaatca taacaatgta aatcgcagtt atattttaga
    70321 ggcgattaaa gatagcttga aacgcttaat tgtcccttcg atagagcgtg aaatccatgc
    70381 tgatttaact gaaaaagctg aaaatcatgc aatagatgtt tttagtgaaa acttaagaaa
    70441 tctattactg caacctccaa tgaaaggtaa acaaatatta ggcgtagatc cagcatttag
    70501 aacaggttgt aaattagcag tcattaaccc attcggtact tttatagcaa aaggtgtgat
    70561 ttatccgcat ccaccagttt ctaaaaaaga ggcagcagag aaggattttg tacaaatggt
    70621 taaagcgtat gatgtgcaat taattgcaat tggcaatggt actgcaagtc gtgaaacaga
    70681 acattttgtt gcagatttaa ttaaaaagca tcagttgcca gtacaattca tcattgtcaa
    70741 tgaagcgggc gcttcagtat actcagcatc agaaattgct agagatgaat ttcctgattt
    70801 tcaagtggaa gaaagaagtg ccgtatcaat tggacgacgc gttcaagatc cattaagtga
    70861 attagttaaa atcgatccta aatctattgg tgtcggacaa taccagcatg atgttaatca
    70921 aaaagcacta gaaaatgcat taactttcgt tgttgaaaca gcagttaacc aagtaggtgt
    70981 tgatgtcaat acagcatcat catcattatt acaatatgtg tctggtctaa gttcgcaaat
    71041 tgctaaaaat ataattgcat atagagaaga aaatggcgca ataaagcata ataaagagtt
    71101 aagcaagatt aagcgtttgg gtgctaaaac ttttgagtag agtataggat ttttaagaat
    71161 agtagacggg tcagagccat tggataatac atcgattcat cccgaaagct ataaagtaac
    71221 gtatcagtta ttagataaac tcggatttgg tggtaatgat ttaggtagtg atgcattaaa
    71281 agctaaatta aattcattgg atatggatga actggcgatt gaattacaag tcggtgtacc
    71341 tacattagag gatattatta aatccttgaa agcacctaat cgagatccaa gggacgagtt
    71401 tgaaacacca atccttaaat ccgatgtgct atcaattgaa gatttaaaag aaggaatgaa
    71461 attgagtggc acggttagaa atgtagtaga ttttggagca tttgtagata taggtgttaa
    71521 acaagatgga cttgtccatg tttcgaaact ttctaagaag ttcgtaaaaa atccaatgga
    71581 tattgtcagt gtcggtgata tcgttgacgt ttggatttac agtattgaca aaaataaaga
    71641 caaagtatca ttaacgatga ttgatccaca tgaataatga caaacttcag cgaatggtcg
    71701 agaacctttc ggaagaaaaa tttggacgta cgtttcaaca tcgtgcatat tttaataaaa
    71761 ggttacgtac aacaggcgga cgttatcttt taaagtctca tgatattgag attaatccaa
    71821 aacaatatga gcattatgga gaagatgctg ttgtaaaaat tatactgcac gaattgtgtc
    71881 attatcactt gcatattgct ggtaaaggtt atcagcataa agatcgagat tttaaacgat
    71941 tgagccaaca ggttggagca cctagatttt gtaacagcat cgaaagctat caacaacggg
    72001 caaattatga atattattgc actaaatgtc atgcaaaata tatacgtatc cgtaaagttg
    72061 atacgactcg tatgcgttgt ggacattgca atggaaaatt aagaatgaaa aaacaattga
    72121 aataagtaca atacaaatag agctaattca tgtatatgta agattattta tgagttgtat
    72181 ggaatttata tagaatttat cagtgagtta ttttgattgg taaacatagt aattgaataa
    72241 gaagaaattg tgtttataaa atgtttaaag ccgagattgt cgtaatttaa tacttcgcaa
    72301 tctcggcttt tctacttatg atataggtaa tggcataaat tgtggcttga taggatgaaa
    72361 ttaaattgat tagattaaaa atcctggcgt attttagtaa tgaataatat ataaacttta
    72421 ccaaattaaa gctttaaagt ggcataaaag ataattatac atttttaatg acattttgat
    72481 tgaacttttc tattgactgt acagcttata gttagtatga ttaaacacgt tccttaaaaa
    72541 agaacaaatt aaataaactt tgatttgaaa gacagtcaat tctaaattta atttataaaa
    72601 tatgttgaca ataagttctt aatgttttat tataaatagc gttgtgttaa caactgataa
    72661 cacaaattaa tttaatgtaa aaaaacgatt aaaagttatt gacaaatatt tttaaacata
    72721 atataataaa taacgttcta aaaaacgaag atgttttaaa tgcctatatc cgcggtcgtg
    72781 gcggaatggc agacgcgcta ggttgagggc ctagtgggag aaatcccgtg gaggttcaag
    72841 tcctctcggc cgcatcaaaa ttcttaatta aataagcggg tgtagtttaa tggcaaaacc
    72901 tcagccttcc aagctgatgt tgtgggttcg attcccatca cccgctccat agataatttt
    72961 aatgaacatt gaaaactgaa tgacaatatg tcaacgttaa ttc
//
LOCUS       DAHHOJ010000002        53243 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.002, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000002 DAHHOJ010000000
VERSION     DAHHOJ010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 53243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53243
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.002"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(58..339)
                     /locus_tag="NBH32_000078"
     CDS             complement(58..339)
                     /locus_tag="NBH32_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1513722.1"
                     /translation="MTYHERVLALRAESKRTAFDFRFEDLFSKEEWLSMSLAERQKAE
                     KAFRHEVKNMDDVRMPFSSVHDAQVKLYNVVYSYNGIKRNFKQVENEGF"
     gene            628..1323
                     /locus_tag="NBH32_000079"
     CDS             628..1323
                     /locus_tag="NBH32_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1513723.1"
                     /translation="MTVLTDKVAVVTGAGSGIGEAIATLLHEEGVKVILAGRNKDKLQ
                     NVANQLAQDSVKVVPTDVTKKEEVDELIKIAQQTFGGLDIVINSAGQMLSSKITDYQV
                     DEWDSMIDVNIKGTLYTAQAALPTMLEQSSGHLINIASISGFEVTKSSTIYSATKAAV
                     HTITQGLEKELAKTGVKVTSISPGMVDTAITAAYNPTDRKKLEPQDIAEAVLYALTQP
                     KHVNVNEITVRPV"
     gene            1575..1997
                     /locus_tag="NBH32_000080"
     CDS             1575..1997
                     /locus_tag="NBH32_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1513724.1"
                     /translation="MTKKHVFIIIGVILCICIVATVIYLKVKYDEKEKQKSIYYKEQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKTGPMGDAVIEGYINENKEDNFVAYGSPEHNYQ
                     FGGSLIKSKNLSTLLKPAHQTKTPDDIKKELDSKKNDR"
     gene            2264..2683
                     /locus_tag="NBH32_000081"
     CDS             2264..2683
                     /locus_tag="NBH32_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1513725.1"
                     /translation="MSKKKILILTSIMLIILISVASIYFKMKYDEKEKQKSIYYKEQQ
                     ARITLYLKHNTKEPNTIKSVHFTKLETSPMGSAVIEGYINENKKDDFVAYASPENNFQ
                     FVGDLIKSERLSELLKPAHQLKSPDGIKKELNKKKSY"
     gene            2701..3126
                     /locus_tag="NBH32_000082"
     CDS             2701..3126
                     /locus_tag="NBH32_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1513726.1"
                     /translation="MSKKHGFIIIGVILCICIVASVIYLKMKYDEKEKQKEIYYKGQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLKRGPMGDAVIEGYINENKEADFVAYGSPEENYQ
                     FTGGLTGSNEVLGKLKNAENLKSPEKIKEEINKKKNTKQ"
     gene            3579..4001
                     /locus_tag="NBH32_000083"
     CDS             3579..4001
                     /locus_tag="NBH32_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCD1513727.1"
                     /translation="MPYNYKKQNGELMSVMSQGEKFIHQSPVNDELSALIKLLISKIN
                     GCHYCVDIHKKELKELGVTQMKIDEVLSFRHLDLFTDQEKVTLEFAEMLNSIKDFKKF
                     EIIDRLKSFYDEEQIIDLVFVVNQINGWNRLNIISDKL"
     gene            complement(4113..5651)
                     /locus_tag="NBH32_000084"
     CDS             complement(4113..5651)
                     /locus_tag="NBH32_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HCD1513728.1"
                     /translation="MTSKHQQKGSIVNRFLNSVEKIGNKLPDPSVLFFLMCIGLAIMT
                     WVISLFNVSVKHPGTHQTIYIKNIISHDGFTMIMNDTIKNFSEFPALGLVLAVMIGIG
                     VAEKTGYFDKLMISVVNRAPRFLILPTIILIGILGSTAGDAATIILPPLAAMLFIKIG
                     YHPIAGLTMAYASAVGGFAANIVVGMQDALVYSFTEPATRIVSDSIKTNVAMNWYFIA
                     ASVVVLLPTILLVTTKLIIPRLGKYDDSLMHDDHEETSSHITDKEAHALKWANISFIV
                     TIILLIITAIPEHSFLRNAKTGSLLDDAPLINGVGLIILVVFLVPGLVYGILSKEIKN
                     TKDLGKMFGDAVGSMGTFIVIVFFAAQLLAYLKWSNLGIIAAVKGAKLLEHQNGIVLI
                     LGIIVLSAMVNMLIGSASAKWGILGPIFVPMLILIGFHPAFTQVIYRVGDSITNPITP
                     MMPYLPLLLTYAQKYDKRMKLGALLSSLMPYSIALSIVWTVFVIIWFLLGIPVGPGGP
                     IFVK"
     gene            5886..6704
                     /locus_tag="NBH32_000085"
     CDS             5886..6704
                     /locus_tag="NBH32_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1513729.1"
                     /translation="MKRLENKVAVVTGASTGIGQASAIALAQEGAYVLAVDIAEAVSE
                     TVDKIKSNGDNAKAYNVDISDEQQVVDFVSDIKEQFGRIDVLFNNAGVDDASGRIHEY
                     PIDVYDKIMNVDMRGTFLMTKMMLPLMMNQGGSIVNTSSFSGQAADLYRSGYNAAKGA
                     VINFTKSIAIEYGRDGIRSNAIAPGTIETPLVDKLTGTSEDDAGKTFRENQKWMTPLG
                     RLGKPEEVGKLVVFLASDDSSFITGETIRIDGGVMAYTWPGEMLSDDSWKRTLE"
     gene            6968..7333
                     /locus_tag="NBH32_000086"
     CDS             6968..7333
                     /locus_tag="NBH32_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513730.1"
                     /translation="MLKEKESFRLLYQAIREIADKIGDNQLETNSVSLLLLDFDFEHE
                     VFDELYLAILKYLNTVSIENISHSELLNLIENTIPEDREINTFVKNKIIIGFANNYFP
                     ELQVLANEIKSDMASSLKQ"
     gene            8212..9033
                     /gene="cntK"
                     /locus_tag="NBH32_000087"
     CDS             8212..9033
                     /gene="cntK"
                     /locus_tag="NBH32_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine racemase CntK"
                     /protein_id="HCD1513731.1"
                     /translation="MNRQVIEFSKYNPSGNMTILVHSKHDASEYASIANQLMAATHVC
                     CEQVGFIESTQNDDGNDFHLVMSGNEFCGNATMSYIHHLQESHLLKDQQFKVKVSGCS
                     DLVQCAIHDCQYYEVQMPQAHRVVPTTINMGNHSWKAIEIIYETYVHYVIPVKQVTTE
                     IQHLVESYVRKQQWNHKYKTVGMMLFDEQRQFLQPLIYIPEIQSLIWENSCGSGTASI
                     GVFNNYQRNDACKDFTVHQPGGSILVTSKRCHQLGYQTSIKGQVTTVATGKAYIE"
     gene            9044..9862
                     /gene="cntL"
                     /locus_tag="NBH32_000088"
     CDS             9044..9862
                     /gene="cntL"
                     /locus_tag="NBH32_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-histidine (S)-2-aminobutanoyltransferase CntL"
                     /protein_id="HCD1513732.1"
                     /translation="MNNFNNEIKLILQQYLEKFEAHYERVLQDDQYIEALETLMDDYS
                     EFILNPIYEQQFNAWRDVEEKAQLIKSLQYITAQCVKQVEVIRARRLLDGQASTTGYF
                     DNIEHCIDEEFGQCSIASNDKLLLVGSGAYPMTLIQVAKETGASVIGIDIDPQAVDLG
                     RRIVNVLAPNEDITITDQKVSELKDIKDVTHIIFSSTIPLKYSILEELYDLTNENVVV
                     AMRFGDGIKAIFNYPSQETAEDKWQCVNKHMRPQQIFDIALYKKAAIKVGITDV"
     gene            9855..11156
                     /gene="cntM"
                     /locus_tag="NBH32_000089"
     CDS             9855..11156
                     /gene="cntM"
                     /locus_tag="NBH32_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_096659686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine dehydrogenase CntM"
                     /protein_id="HCD1513733.1"
                     /translation="MSKLLMIGTGPVAIQLANICYLKSDYEIDMVGRASTSEKSKRLY
                     QAYKKEKQFEVKIQNEAHQHLEGNFEINRLYKDVKNVKGEYETVVMACTADAYYDTLQ
                     QLSLETLQSVKHVILISPTFGSQMIVEQFMSKFNKDIEVISFSTYLGDTRIVDKEAPN
                     HVLTTGVKKKLYMGSTHSNSTMCQRISALAEQLKIQLEVVESPLHAETRNSSLYVHPP
                     LFMNDFSLKAIFEGTDVPVYVYKLFPEGPITMTLIREMRLMWKEMMAILQAFRVPSVN
                     LIQFMVKENYPVRPETLDEGDIEHFEILPDILQEYLLYVRYTAILIDPFSQPDENGHY
                     FDFSAVPFKQVYKNEQDVVQIPRMPSEDYYRTAMIQHIGKMLGIKTPMIDQFLTRYEA
                     SCQAYKDMHQDQQLSSQFNTNLFEGDKALVTKFLEINRTLS"
     gene            11299..12897
                     /gene="cntA"
                     /locus_tag="NBH32_000090"
     CDS             11299..12897
                     /gene="cntA"
                     /locus_tag="NBH32_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylopine-dependent metal ABC transporter
                     substrate-binding protein CntA"
                     /protein_id="HCD1513734.1"
                     /translation="MRKLTKMSAMLLASGLILTGCGGNKGLEEKKENKQLTYTTVKDI
                     GDMNPHVYGGSMSAESMIYEPLVRNTKDGIKPLLSKKWDVSEDGKTYTFHLRDDVKFH
                     DGTPFDADAVKKNIDAVQENKKLHSWLKISTLIDNVKVKDKYTVELNLKEAYQPALAE
                     LAMPRPYVFVSPKDFKNGTTKDGVKKFDGTGPFKLGEHKKDESADFNKNDQYWGEKSK
                     LNKVQAKVMPAGETAFLSMKKGETNFAFTDDRGTDSLDKDSLKQLKDTGDYQVKRSQP
                     MNTKMLVVNSGKKDNAVSDKTVRQAIGHMVNRDKIAKEILDGQEKPATQLFAKNVTDI
                     NFDMPTRKYDLKKAESLLDEAGWKKGKDSDVRQKDGKNLEMAMYYDKGSSSQKEQAEY
                     LQAEFKKMGIKLNINGETSDKIAERRTSGDYDLMFNQTWGLLYDPQSTIAAFKAKNGY
                     ESATSGIENKDKIYNSIDDAFKIQNGKERSDAYKNILKQIDDEGIFIPISHGSMTVVA
                     PKDLEKVSFTQSQYELPFNEMQYK"
     gene            12910..13845
                     /locus_tag="NBH32_000091"
     CDS             12910..13845
                     /locus_tag="NBH32_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1513735.1"
                     /translation="MFKFILKRIALMFPLVIVVSFMTFLLTYITNENPAVTILHAQGT
                     PNVTPELIAETNEKYGFNDPLLIQYKNWLLEAMQFNFGTSYITGDPVAERIGPAFMNT
                     LKLTIISSVMVMITSIILGVVSALKRGKFTDRAIRSVAFFLTALPSYWIASILIIYVS
                     VKLNILPTSGLTGPESYILPVIVITIAYAGIYFRNVRRSMVEQLNEDYVLYLRASGVK
                     SITLMLHVLRNALQVAVSIFCMSIPMIMGGLVVIEYIFAWPGLGQLSLKAILEHDFPV
                     IQAYVLIVAVLFIIFNTLADIINALLNPRLREGAR"
     gene            13842..14711
                     /locus_tag="NBH32_000092"
     CDS             13842..14711
                     /locus_tag="NBH32_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1513736.1"
                     /translation="MIILKRLLQDKGAVVALGIIVLYVFLGLAAPLVTFYDPNHIDTA
                     NKFAGMSFQHLLGTDHLGRDILTRLIYAIRPSLLYVFVALFVSVLIGSILGFLSGYFQ
                     GFVDALIMRACDVMLAFPSYVVTLALIALFGMGAENIIMAFILTRWAWFCRVIRTSVM
                     QYTASDHVRFAKTIGMNDMKIIHKHIMPLTLADIAIISSSSMCSMILQISGFSFLGLG
                     VKAPTAEWGMMLNEARKVMFTHPEMMFAPGIAIVIIVMAFNFLSDALQIAIDPRISSK
                     DKLRSVKKGVVQS"
     gene            14708..15523
                     /locus_tag="NBH32_000093"
     CDS             14708..15523
                     /locus_tag="NBH32_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1513737.1"
                     /translation="MTLLTVKHLTITDTWTDQPLVSDVNFTLTKGETLGVIGESGSGK
                     SITCKSIIGLNPERLGVTGDITFDGKPMLSLSEAQLKKYRGKDIAMVMQQGSRAFDPS
                     TTVGKQMFETMKVHTSMSTQEIEKTLIEYMDYLSLKDPKRILKSYPYMLSGGMLQRLM
                     IALALALKPKLIIADEPTTALDTITQYDVLEAFIDIKKHFDCAMIFISHDLTVINKIA
                     DRVVVMKNGQLIEHGTRESVLHHPKHVYTKYLLSTKKKINDHFKHVMRGDVHD"
     gene            15516..16265
                     /locus_tag="NBH32_000094"
     CDS             15516..16265
                     /locus_tag="NBH32_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1513738.1"
                     /translation="MIKIKDVEKSYQSAHVFKRRRTPIVKGVSFECPIGATIAIIGES
                     GSGKSTLSRMILGIEKPDKGCVTLNDHPMHKKKVRRHQIGAVFQDYTSSLHPFQTVRE
                     ILFEVMCQCDGQPKDVMEVQAITLLEEVGLSKAYMDKYPNMLSGGEAQRVAIARAICI
                     NPKYILFDEAISSLDMSIQTQILDLLIHLRETRQLSYIFITHDIQAATYLCDQLIIFK
                     NGKIEEQIPTSALHKSDNAYTRELIEKQLSF"
     gene            16277..17470
                     /locus_tag="NBH32_000095"
     CDS             16277..17470
                     /locus_tag="NBH32_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1513739.1"
                     /translation="MKGAMAWPFLRLYILTLMFFSANAILNVFIPLRGHDLGATNTVI
                     GIVMGAYMLTAMVFRPWAGQIIARVGPIKVLRIILIINAIALIIYGFTGLEGYFVARV
                     MQGVCTAFFSMSLQLGIIDALPEEHRSEGVSLYSLFSTIPNLIGPLVAVGIWNANNIS
                     LFAIVIIFIALTTTFFGYRVTFAEQEPDTSDKIEKMPFNAVTVFAQFFKNKELLNSGI
                     IMIVASIVFGAVSTFVPLYTVSLGFANAGIFLTIQAIAVVAARFYLRKYIPSDGMWHP
                     KYMVSVLSLLVIASFVVAFGPQVGAIIFYGSAILIGMTQAMVYPTLTSYLSFVLPKVG
                     RNMLLGLFIACADLGISLGGALMGPISDLVGFKWMYLICGMLVIVIMIMSLLKKPTPR
                     PANSL"
     gene            complement(17936..18685)
                     /locus_tag="NBH32_000096"
     CDS             complement(17936..18685)
                     /locus_tag="NBH32_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513740.1"
                     /translation="MSNTNKHYIEEEYATEQSRFFKRDIGFIFLYIFLVNILPIIIVF
                     LTFGLVAIFTQQAPWDFSFPISMNISYLLAVILSLAIFRRMHEDAFIPMIRSQIVAST
                     KYKWHILISFTLSIVLFLLLNHFEIFALNVDSFTVFDIYPLIREYGSISLIAIFSLTI
                     ILNTLICQLIFRHILIQELSRLIPLKITIVLSVILETLYYYPYINTWWEFIPALILAS
                     SATYLYLKSNRNFMVSYFYQLAVILVLHIFM"
     gene            18880..20457
                     /locus_tag="NBH32_000097"
     CDS             18880..20457
                     /locus_tag="NBH32_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding glutamate synthase family protein"
                     /protein_id="HCD1513741.1"
                     /translation="MTFLTVMQFIVNIIIIGFLLTVIVIGLIWLIKDKRQSQHSVLRN
                     YPLLARIRYISEKMGPELRQYLFSGDNEGKPFSRNDYKNIVLAGKYNSRMTSFGTTKD
                     YQDGFYIQNTMFPMQRNEISVDNTTLLSTFIYKIANERLFSREEYRVPTKIDPYYLSD
                     DHAIKLGEHLKYPFILKRIVGQSGMSYGALGKNAITALSKGLAKAGTWMNTGEGGLSE
                     YHLKGNGDIIFQIGPGLFGVRDKEGNFSEGLFKEVAQLSNVRAFELKLAQGAKTRGGH
                     MEAEKVNEEIAKIRNVEPYKTINSPNRYEFIHNAEDLIRFVDQLQQLGQKPVGFKIVV
                     SKVSEIETLVRTMVELDKYPSFITIDGGEGGTGATFQELQDGVGLPLFTALPIVSGML
                     EKYGIRDKVKLAASGKLVTPDKIAIALGLGADFVNIARGMMISVGCIMSQQCHMNTCP
                     VGVATTDAKKEKALIVGEKQYRVTNYVTSLHEGLFNIAAAVGVSSPTEITADHIVYRK
                     IDGELQTIHDYKLKLIS"
     gene            complement(20625..21080)
                     /locus_tag="NBH32_000098"
     CDS             complement(20625..21080)
                     /locus_tag="NBH32_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1307 domain-containing protein"
                     /protein_id="HCD1513742.1"
                     /translation="MKKLCSLIVVALVCIIALSACGKEQTKTYEGDVSGKHVLTSITY
                     KNDKVLKQSTINTIKYDDLGMDKDEAKKLFAKSESIFKDLKGVKYKVDYKDKKAIEHL
                     DIDYTEVDMKKLNKRLGVSTKENKDISFEKLEKQLKHRGLKEKDKMDDK"
     gene            21439..21696
                     /locus_tag="NBH32_000099"
     CDS             21439..21696
                     /locus_tag="NBH32_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1513743.1"
                     /translation="MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLE
                     DWKSIQETIYLESTGTMDKVREREKDNSGTTNIDDIDWDNL"
     gene            21696..21962
                     /locus_tag="NBH32_000100"
     CDS             21696..21962
                     /locus_tag="NBH32_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1513744.1"
                     /translation="MSNYTVKIKNSAKSDLKKIKHSYLKKSFLEIVETLKNDPYKITQ
                     SFEKLEPKYLERYSRRINHQHRVVYTVDDRNKEVLILSAWSHYD"
     gene            22317..23393
                     /locus_tag="NBH32_000101"
     CDS             22317..23393
                     /locus_tag="NBH32_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1513745.1"
                     /translation="MNQSVKLLKHLTDVNGIAGYEMQVKEAMRNYIEPVSDQIIEDNL
                     GGIFGKKNAENGQYSIMISGHMDEVGFMVTKIDKHGFISFTPVGGWWNQVMLSQKVTI
                     TTDSGKEIRGIIGSKPPHVLTPEERKKPMEIKNMFIDIGVSSKGEAEEAGVEVGNMVT
                     PYSEFEVLANNKYLTAKAFDNRYGCALAIEVLQRLKDKNIGINLYSGATVQEEVGLRG
                     AKVAANTIKPDLAIAVDVGIAYDTPGMSGQTSDSKLGGGPVVIMMDATSIAHQGLRKH
                     IKDVAKKHNIEVQWDTTPGGGTDAGSIHVANEGIPTMTIGVTLRYMHSNVSVLNVDDY
                     ENSIRLVTEIVRSLNDETYKNIMW"
     gene            23739..24401
                     /locus_tag="NBH32_000102"
     CDS             23739..24401
                     /locus_tag="NBH32_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1513746.1"
                     /translation="MLLEIKDLVYEASDRVILNHINLKVDKGETIAIIGPSGSGKSTF
                     QKIICNLISPTSGTLYFKDKPYDDYEPEILRRHISYLMQQSDLFGETIEDNMIFPSLA
                     RNDKFDKKRAKQLIKDVGLGHYQLSSKIEHMSGGERQRIAIARQLMYTPDILLLDEST
                     SALDIHNKEKIENIIFKLADQGVAIMWITHSDDQSMRHFQKRITIVDGQISNIEELNQ
                     HE"
     gene            24394..25170
                     /gene="fetB"
                     /locus_tag="NBH32_000103"
     CDS             24394..25170
                     /gene="fetB"
                     /locus_tag="NBH32_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCD1513747.1"
                     /translation="MSNTALGLTALLLVIPIIISYKEGLHIIKDLIVATLRAVVQLII
                     LGFLLHYIFKINDKWLLVLCVFVIIVNASWNTISRSSPVMHHVFWISFVAIFVGTALP
                     LAGTIATGAIQFTANEVIPIGGMLANNGLIAINLAYQNLDRAFVQDGTNIESKLSLAA
                     TPKLASKGAIRESIRLAIVPTIDSVKTYGLVSIPGMMTGLIIGGVPPLQAIKFQLLVV
                     FIHTTATIMSALIATYLSYGQFFNARHQLVARNTDVKSES"
     gene            25564..26751
                     /locus_tag="NBH32_000104"
     CDS             25564..26751
                     /locus_tag="NBH32_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1513748.1"
                     /translation="MSIMRLFTFILSIFIVGMVEMMVAGIMNLMSQDLHVSEAVVGQL
                     VTMYALTFAICGPILVKLTNRFSSRPVLLWTLLIFIIGNGIIAVAPNFSILVVGRIIS
                     SAAAALIIVKVLAITAMLSAPKNRGKMIGLVYTGFSGANVFGVPIGTVIGDLVGWRYT
                     FLFLIIVSIIVGFLMMIYLPKDQEIQRGSVNHEAPSHENHVTSKILRPAEVAKYLIIT
                     FLVLIANSVTFVFINPLILSNGHDMSFVSLALLVNGIAGVIGTSLGGIFSDKITSKRW
                     LMISVSIFIVMMLLMNLILPGSGLLLAGLFIWNIMQWSTNPAVQSGVIQHVEGDTSQV
                     MSWNMSSLNAGIGVGGIIGGLVMTHVSVQAITYTSAIIGALGLIVVLTLKNNHYAKTF
                     KSS"
     gene            complement(26813..28165)
                     /locus_tag="NBH32_000105"
     CDS             complement(26813..28165)
                     /locus_tag="NBH32_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase/lipase family protein"
                     /protein_id="HCD1513749.1"
                     /translation="MKINTTGGQIYGITQDRLDIFLGIPYAEPPVHDNRFKHSTLKTQ
                     WSEPIDATEIQPIPPQPDNKLEDFFSSQSATFTEHEDCLYLNIWKQHNDQSKKPVIIY
                     FYGGSFENGHGTAELYQPAHLVQNNDIIVITCNYRLGALGYLDWSYFNKGFHSNNGLS
                     DQINVIKWVHQFIESFGGDANNITLMGQSAGSMSILTLLKIPDIEPYFHKVVLLSGAL
                     RLDTLESARNKAQHFQKMMLDYIDTDDVTSLSTDDILMLMAKLKQSRGPSKGLDLIYA
                     PIKTDYIQNNYPTTKPIFACYTKDEGDIYITSEQKKLSPQRFIDIMELNDIPLKYEDV
                     QTAKQQSLAITHCYFKQPMKQFLQQLNIQDSNAQLWLAEFAWHDTSSAHYRSAYHILD
                     MIFWFGNLQILSAHQYPITDHLKFLSRQMQNDLANFAKSGKMPWPIYHNERRYYRTYQ
                     "
     gene            complement(28472..30109)
                     /locus_tag="NBH32_000106"
     CDS             complement(28472..30109)
                     /locus_tag="NBH32_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1513750.1"
                     /translation="MNKDKQLHNDKINLSQLVLLGLGSLIGSGWLFGAWEASSIAGPA
                     AIISWVLGFLVIGTIAYNYIEIGTMFPQSGGMSNYAQYTHGSLLGFIAAWANWVSLVT
                     IIPIEAVSAVQYMSSWPWHWAKPMRYLMENGSISTYGLLAVYLIIVIFSLLNYWSVKL
                     LTSFTSLISVFKLGVPMLTIIMLMLSGFDTSNYGHSASTFMPYGSAPIFAATTASGII
                     FSFNSFQTIINMGSEIKNPEKNIARGIAISLSISAVLYIILQSTFITSMPQSMLQHSG
                     WNGINFNSPFADLAILLGINWLAILLYIEAFVSPFGTGVSFVAVTGRVLRAMEKNGHI
                     PKFLGKMNEKYHIPRVAIIFNAIISMIMVTLFRDWGTLAAVISTATLVAYLTGPTTVI
                     ALRKMGPTMTRPFRAKILKVMAPLSFVLASLAIYWAMWPTTAEVILIIILGLPIYFFY
                     EYRMNWRNTKKQIGGSLWIIVYLIVLSILSFIGSKEFKGLNMIHYPFDFIVIIIVALI
                     FYYIGTTSSFESVYFRRATRINTKMHESLNNESKSSH"
     gene            30290..30889
                     /locus_tag="NBH32_000107"
     CDS             30290..30889
                     /locus_tag="NBH32_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family protein"
                     /protein_id="HCD1513751.1"
                     /translation="MSEEQSNEQVEAFLNKESQWQEEYKYLRALIFNETELEEAYKWM
                     HPCYTLNNKNVVLIHGFKNYVALLFHKGAILEDKYHTLIQQTEKVQAARQLRFENLTE
                     IQARSEEIKYYLAEAIKAEKAGKKVEMKKTEEYVIPKELEAKFEEMPQLESSFYKLTP
                     GRQHQYIYHIGQAKRSETRQKRVEKYINQILEGKGMHDK"
     gene            30924..31033
                     /locus_tag="NBH32_000108"
                     /pseudo
     CDS             30924..31033
                     /locus_tag="NBH32_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501207.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            31113..31232
                     /locus_tag="NBH32_000109"
     CDS             31113..31232
                     /locus_tag="NBH32_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513752.1"
                     /translation="MGFCPPVTMDACALLQKFDFCNNISHFRHFFAIKQPIER"
     gene            31559..32785
                     /locus_tag="NBH32_000110"
     CDS             31559..32785
                     /locus_tag="NBH32_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1513753.1"
                     /translation="MLSIKHLTKIYSGNKKAVDDISLDIQSGEFIAFIGTSGSGKTTA
                     LRMINRMIEATEGQIEIDGKDVRSMNPVELRRNIGYVIQQIGLMPHMTIKENIVLVPK
                     LLKWTKEEKDKRAKELIKLVDLPESFLERYPAELSGGQQQRIGVVRALAAEQDIILMD
                     EPFGALDPITRDTLQDLVKTLQRKLGKTFIFVTHDMDEAIKLADKICIMSEGKVVQFD
                     TPDNILRHPANDFVRDFIGQNRLIQDRPNDKTVEGVMIKPITIQAEATLNDAVHIMRQ
                     KRVDTIFVVDSNNHLLGFLDIEDINQGIRGHKSLRDTMQQHIYTVQIDSKLQDSVRTI
                     LKRNVRNVPVVDDKQRLVGLITRANVVDIVYDTIWGDSEDAVQTEHVGEDTASSKMHE
                     QHTTNVKVRDIGDDKS"
     gene            32782..33417
                     /locus_tag="NBH32_000111"
     CDS             32782..33417
                     /locus_tag="NBH32_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1513754.1"
                     /translation="MIEFLHEHGGQLMSKTLEHFYISIVALLLAIIVAVPIGILLSKT
                     KRTANIILTVAGVLQTIPTLAVLAIMIPIFGVGKTPAIVALFIYVLLPILNNTVLGVQ
                     NIDSNIKEAGKSMGMTQFQLMKDVELPLALPLIIGGIRLSSVYVISWATLASYVGAGG
                     LGDFIFNGLNLYDPLMIVTATVLVTALALGVDALLALVEKWVVPKGLKVSG"
     gene            33434..34375
                     /locus_tag="NBH32_000112"
     CDS             33434..34375
                     /locus_tag="NBH32_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1513755.1"
                     /translation="MKKIKYILVVFVLSLTVLSGCSLPGLGSKSTKNDVKITALSTSE
                     SQIISHMLRLLIEHDTHGKIKPTLVNNLGSSTIQHNALINGDANISGVRYNGTDLTGA
                     LKEAPIKDPKKAMIATQQGFKKKFDQTFFDSYGFANTYAFMVTEETAKKYHLETVSDL
                     AKHSKDLRLGMDSSWMNRKGDGYEGFKKEYGFDFGTVRPMQIGLVYDALNSEKLDVAL
                     GYSTDGRIAAYDLKVLKDDKQFFPPYAASAVATNELLRQHPELKTTINKLTGKISTSE
                     MQRLNYEADGKGKEPAVVAEEFLKKHHYFDKQKGGHK"
     gene            34375..35070
                     /locus_tag="NBH32_000113"
     CDS             34375..35070
                     /locus_tag="NBH32_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1513756.1"
                     /translation="MEGNLLQQLFNYYVTNFGYLWDLFFKHLLMSVYGVLFAALIGIP
                     LGILLARYTKLSGFVITIANIIQTVPVIAMLAILMLVMGLGSETVVLTVFLYALLPII
                     KNTYTGIASVDANIKDAGKGMGMTRNQVLRMIELPLSVSVIIGGIRIALVVAIGVVAV
                     GSFIGAPTLGDIVIRGTNATDGTTFILAGAIPIAIIAIVIDVLLRFLEKRLDPTTRHR
                     KNQSNHRPQSINM"
     gene            35364..36764
                     /locus_tag="NBH32_000114"
     CDS             35364..36764
                     /locus_tag="NBH32_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1513757.1"
                     /translation="MDTSKQFRGDNRLLLGIVLGVITFWLFAQSLVNLVVPLQSTYSS
                     DVGTINIAVSLSALFAGLFIVGAGDVADKFGRVKITYVGLILNVIGSLLIIITPLPAF
                     LIIGRIIQGLSAACIMPSTLAIINEYYIGTRRQRALSYWSIGSWGGSGICTLFGGLMA
                     TYIGWRSIFVVSILLTLLAMYLIKHAPETKAEPIKGMKAEAKKFDVIGLVILVVTMLS
                     LNVIITQTSHFGLVSPLILGLIIVFICSLVGFVYYENKIKHPLVDFSIFKNRGYSGAT
                     ISNFLLNGVAGGALIVINTYYQQQLGFNSSQTGYISLTYLIAVLSMIRVGEKILSQHG
                     PKRPLLLGSGFTVIGLILLSLTFLPEMWYIVSSIVGYLLFGTGLGLYATPSTDTAVAS
                     APDDKSGVASGVYKMASSLGNAFGVAVSGTVYTVLAANLNLNLGGFTGMMFNALLAIV
                     AFLVILLLVPKNQTNL"
     gene            36990..37925
                     /locus_tag="NBH32_000115"
     CDS             36990..37925
                     /locus_tag="NBH32_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCD1513758.1"
                     /translation="MKIAIAGSGALGSGFGAKLFQAGYNVTLIDGYTSHVEAVKQHGL
                     NITINGEAFELNIPMYHFNDQPDESIYDVVFLFPKSMQLKEVMEAMKSHIDNETIVVC
                     TMNGLKHEEVIAQYVAQSQIVRGVTTWTAGLESPGHSHLLGSGPVEIGELVDEGKENV
                     IKVAELLNEAELNGVISKDLYQSIWKKICVNGTANALSTVLECNMASLNESSYAKCLI
                     YKLTQEIVHVATIDNVHLNVDEVFEYLVDLNEKVGAHYPSMYQDLIVNNRKTEIDYIN
                     GAVATLGKKRHIEAPVNRFITDLIHTKESQRHAQD"
     gene            38249..39100
                     /locus_tag="NBH32_000116"
     CDS             38249..39100
                     /locus_tag="NBH32_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1513759.1"
                     /translation="MSKIFVTGATGLIGIKLVQRLKEEGHEVAGFTTSENGQQKLAAV
                     NVKAYIGDILKADTIDQALADFKPEIIINQITDLKNVDMAANTKVRIEGSKNLIDSAK
                     KHDVKKVIAQSIAFMYEPGEGLANEETSLDFNSTGDRKVTVDGVVGLEEETARMDEYV
                     VLRFGWLYGPGTWYGKDGMIYNQFMDGEVTLSDGVTSFVHLDDAVETSIQAIHFENGI
                     YNVADDAPVKGSEFAEWYKEQLGVEPNIDIQPAQPFERGVSNEKFKAQGGTLIYKTWK
                     DGMNPIK"
     gene            complement(39191..40687)
                     /locus_tag="NBH32_000117"
     CDS             complement(39191..40687)
                     /locus_tag="NBH32_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase FLP"
                     /protein_id="HCD1513760.1"
                     /translation="MTTKKLYFLSISIIILVAISIAIYITLNSNTKTRLTNDSQQQID
                     TIIEHDLQKGHIPGASILIVKNGKVFLNKGYGYQDVDKKVKASPTTKYEIASNTKAFT
                     GLAILKLAQEGRLNLNDAVSKHVPHFKMNYNGQNETITIKQLLAQTSGIPSDITSEDS
                     VTNKNNRLNDVTRAIMGDELHHKPGEEFEYSNMNYDLLGLIIQNVTKQSYTKYITNSW
                     LKPLNMTHTSFKQTNNKTKHDAIGYELQGSTPVVSKPEFNLWDTPSAYMMTSTEDLEH
                     WIKFQLNPPDKYKSLVQQSHKNLSSTIGEPNANAYASGWFTNNDEHLVFHSGTLDNFS
                     SFILLNPKQNYGIVVLANLNSEYVPKLVEHLNTQIVNHKRYSTVASMLNQYKNQFNIV
                     TVLMTTLILLAFIFSAYRAWQMRHGKIILRKSKLTTFSSWLTLCLCIAIALILYALPY
                     LILGSNNWSFVLTWLPIEIKLALITILIALFSTLIVILLFLHTKITKT"
     gene            40920..42749
                     /locus_tag="NBH32_000118"
     CDS             40920..42749
                     /locus_tag="NBH32_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1513761.1"
                     /translation="MFNQFKRLIIGQPKKNRELKDEKISKFKGLAILSSDALSSVAYG
                     PEQILITLSVVGAVATWYTLPIAGAVLILLAALIMSYRQIIYAYPKGGGAYMVSKTNL
                     GEKWGLLAGGSLLVDYILTVAVSISSGADAFVAAFPSLYGHKVLIACLLVLFILILNL
                     RGLTESATVLSYPVYLFIIGLVILIFIGTFRVATGDIQPHMHASVGTAVPGVTLFLLL
                     KAFSSGASSLTGVEAISNAVTNFREPSANNAVKTLIAMGSILAFLLVGIVGLAYVYGI
                     MPQTETTVLSQLAMQIFGDNAAFYFVQATTVMILVLAANTGFTAFPMLAASMSKDKYM
                     PRMFTVRGDRLGYSNSIIILGVLAIILIIVFDGMTEDLIPLYAVGVFIPFTLAQFGMV
                     IKWIHERPKNWLSKLSVNLLGGIVTFIVFMILLITKFSQVWPILIFLPFVVIFFLKIN
                     KHYRDIAEQLRSDIDVLNVDVVDRNLAIVPITSITTAVDKSIYYAQMLANNDVIAVHV
                     SFGDEDEKAFQEKWKRHFPDVRLVILHSEYRSIIRPISRFIDKINRKANDQNYMITVV
                     IPEFITKKRWHNLLHNQTSLRMKLYLIYQKNVNVCTIPFKLKK"
     gene            complement(42889..44967)
                     /locus_tag="NBH32_000119"
     CDS             complement(42889..44967)
                     /locus_tag="NBH32_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD1513762.1"
                     /translation="MALLEAFLIFIFAVIISSVINNRFPQIPTAFIQIALGVVIFIIP
                     IQVDFQFNSEVFMFAVIAPLLFVGGTHVSRTKLLEYRKPILLMSMALVFATVVGVGYF
                     IHWIWPALPMPAAFAIAAILCPTDAVAVSAITRGKLLPKGSMTILEGESLLNDAAGII
                     SFKIAVTALVTGTFSLFQAVEQFIISTILGVLIGAIIGFVIVRIRIDLTANKGLKDNN
                     TLTFIQLLTPFVVYFLAEEVHASGIIAVVIAGLIHGLERDRLIRAQTELQMNYHQIWN
                     TFSYALNGFVFVVLGFMIPTVVIDIFQTEPDNFSFLIVITILIAIAIYACRFIWVYFW
                     YKDFYFPKNIQSYLDEEHDSHETPPSRVRYAFIMTMCGIHGTISLSMALTLPFIITQG
                     QAFEYRNDLLFIASFMVLISLILAQIVLPLITPSAEDTTFKGMTYQSAKIFIVQKVIE
                     HFKNESKKDKNDTNYRPVLNQYYGELLFLLNSEPDNQNTKELKRLEDIAKVIETSTLE
                     RLIDKGKATYQDINNYRNIVELTETHRTASFVEKFANFFKMLWLRLKAMKHYKALNKE
                     SKKQEFENSFKDVQKIMRIVNHNIILRLKEEQNSTNVLEVSLVINHYYDMSRSLKWRA
                     QRRKERQENSNQIIPQAMFHNHKLEALYLQRHLLDELIRKNKINNIVAAQIRENINYN
                     EIVLSLQSKH"
     gene            complement(45105..46514)
                     /locus_tag="NBH32_000120"
     CDS             complement(45105..46514)
                     /locus_tag="NBH32_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1513763.1"
                     /translation="MARKLNRELNNRHIQLIAIGGAIGTGLFLGSGQTISLTGPSLLF
                     TYMIIGVVLFAFMRALGELLLSNTRFNSFVDIANEYLGPFGGFVIGWTYWLCWIVSSM
                     SDLTAMGQYFAFWYPQVPNWITVLFIVLILISFNLLGARLFGELEFWFSIIKVVTIIA
                     MVIVGLVLIFFSFKTHYGHASFTNLISHGGMFPGGTFGFLMSFQIAVYSFIGIELIGV
                     TAGETKDPEKTLPKAINNVPIRILLFYIGGLLVIMSVIPWNDIDPNSSPFVKLFTLIG
                     VPFAAGVVNFVVLTAAASATNSGIYSNSRILFGLSQQGLGPKVLNKTNSHGVPYLSML
                     VSSIALLIAALLNYIFPNAIQLFIYVTTLSTVLFLVVWAMIIVAYLMYLKKHPEAHKN
                     SKFKLIGGKPIAYIILAFFFFVFILLFFSDETRAAIYISPFWFIFLFFFYKKYKTNAE
                     KLAYEQRQNDSGHFRYDNQ"
     gene            46800..47606
                     /locus_tag="NBH32_000121"
     CDS             46800..47606
                     /locus_tag="NBH32_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1513764.1"
                     /translation="MKIGTIADLHIDRHNKKTSEDYLEALVEIVKYKKLDILLIAGDI
                     SNHYQLTHQFITQLTKQLDIPVKFVPGNHDLWEVESMTTQDIWNNYKRMSQCLVGKPF
                     IVNEEWAIIGHTGWYDYSFAAQRFSLDELQKVKHYGATWQDKERISWGISDQNLSKIA
                     VEQVKKDILEVGNRRVILVTHVVTHPDFIVPMPHRIFDFYNAFIGTSDFNPLYAMFDI
                     PYSIMGHVHFRKSVMDDGRCYLCPCLGYPRQWRSEDIYQEINETIQIIEI"
     gene            complement(47669..48325)
                     /locus_tag="NBH32_000122"
     CDS             complement(47669..48325)
                     /locus_tag="NBH32_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001080277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HCD1513765.1"
                     /translation="MNRKILPVKPISQLFPIPMVMGCEGVSAAMMLQYNNQHIPATEI
                     MRHWPTHPNNPHKGYVGHHLFIKLGNYHQTIFPEAYVPFLQKYNPNIVDGTGKSLEDL
                     KGIIDQGQPVLIYHTNLGSKPLLRVFRFDNKPAKQVSNIHVTLLIGYDDYYYYYIDPL
                     WSHIRRGLVLPAIIPNRKQIIKIRKEKMEYSFNSPGRKCIYVQPHSYTIENQQQNKHT
                     "
     gene            48438..48911
                     /locus_tag="NBH32_000123"
     CDS             48438..48911
                     /locus_tag="NBH32_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1513766.1"
                     /translation="MEYQLQQLASLTLVGIKETYENGRQAQQHIAGFWQRCYQEGVIA
                     DLQLKNNVDLVGILGLCIPELDGKMSYMIAVTGDNSADIEKYDVITLASSKYMVFEAQ
                     GAVPKAVQQKMEEVHHYIHQYQADTVKSAPFFELYQDGDTTSEKYITEIWMPVKG"
     gene            complement(49034..50245)
                     /locus_tag="NBH32_000124"
     CDS             complement(49034..50245)
                     /locus_tag="NBH32_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD1513767.1"
                     /translation="MQSSSSKTKQFSFLLLITLGVMTAFGPLTIDMYVPSLPKVQGDF
                     GSTTSEIQLTLSFTMIGLALGQFIFGPLSDAFGRKRIAVSILITFILASGLSVFVTQL
                     PLFLTLRLIQGLTGGGVIVIAKASAGDKFSGNALAKFLASLMVVNGIITILAPLAGGL
                     ALSVATWRAIFTILTIVALIILIGVASQLPKTPKEELKQVNFSSVIKDFGSLLKKPAF
                     IIPMLLQGLTYVMLFSYSSASPFITQKLYNMTPQQFSIMFAVNGVGLIIVSQVVALLV
                     EKLHRHILLIILTIIQVVGVALIILTLTFHLPLWVLLIAFFLNVCPVTSIGPLGFTMA
                     MEERTGGSGNASSLLGLFQFILGGTVAPLVGLKGEFNTSPYMIIIFITAILLVSLQII
                     YFKMIKKQHVA"
     gene            complement(50424..51083)
                     /locus_tag="NBH32_000125"
     CDS             complement(50424..51083)
                     /locus_tag="NBH32_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513768.1"
                     /translation="MKRTDKYRDSYQYDNQNQNHRRQSEDASYRQQYAKGDPEEHPER
                     YYNGRDYRREQILEEENEKSRRSKKWLYIIIAILLIIVAIFVTRALLNNDSDKVSNDP
                     KVSQNYKKQVENQDGQINQQVDNAKENIKNNQKTDDIIKNLQNQIDNLKQQEQNKADS
                     KLTQFYQDQINKLTEANNALKNNASQGKIESMLNDINTKFDSIKSKLESLFKDDNGGA
                     N"
     gene            complement(51143..52285)
                     /locus_tag="NBH32_000126"
     CDS             complement(51143..52285)
                     /locus_tag="NBH32_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1513769.1"
                     /translation="MQLQKIVIAPDSFKESMTAQQVGNIIKQAFTNVYGNTLHYDIIP
                     MADGGEGTTDALMHATGATKYTVIVNDPLMRPIEACYARADEQQIAIIEMAAASGLDL
                     LEKEERNPLYTSSYGTGELIKDALNHGAKTIILGIGGSATNDGGTGMLSALGVKFTDV
                     NGDLLQMNGANLAHIAQIDITNLDSRLKEVTFKVACDVSNPLLGENGATYIYGPQKGA
                     DAKMIPKLDFAMSHYHDKIKMCTGKSVNQIPGSGAAGGMGAALLAFCETTLTKGIDVV
                     FDITDFHQRIKDADLVITGEGRMDYQTIFGKTPVGVALAAKQYHIPVIAICGSLGENY
                     QHVYDFGIDSAYSIISSPSTLEDVLQNSEQNLLNTATDIARILKLQ"
     gene            52553..52939
                     /gene="gtxA"
                     /locus_tag="NBH32_000127"
     CDS             52553..52939
                     /gene="gtxA"
                     /locus_tag="NBH32_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase GtxA"
                     /protein_id="HCD1513770.1"
                     /translation="MKLTQTHAEILKFIIVGGINTLNYYVVYLLLLKLLHIEYMISHI
                     TGFIVAFVISYYLNCYFVYRVKPTWRKFISFPITQLVNVSLQTVLLYVFVSWLNLPAE
                     IAPFAGLVITIPITFILSKWILKDSN"
     gene            53073..53180
                     /locus_tag="NBH32_000128"
     CDS             53073..53180
                     /locus_tag="NBH32_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HCD1513771.1"
                     /translation="MFNLLIDIMTSALSGCLVAFFAHWLRTRNNKKGDK"
BASE COUNT        18073 a         7875 c        10070 g        17225 t
ORIGIN      
        1 cctacaagat ttacatataa aattctatca tgtctgctat atataaacga aatgatatta
       61 gaatccttca ttttcaactt gtttaaaatt acgtttaatg ccgttataag aatatacaac
      121 attatataat tttacttggg cgtcatggac acttgagaag ggcattctta catcgtccat
      181 atttttaact tcgtgtcgaa atgctttttc agctttttgt ctttctgcaa gcgacatact
      241 tagccattct tctttgctaa ataaatcttc gaatcgaaaa tcaaatgcgg ttcttttact
      301 ttctgctctt aatgctaaaa cacgctcatg atatgtcacg ttttattcct ccctctaatc
      361 attcaatcga atgataaatc acagtattaa ctttagtact aatataatgt ttttacaata
      421 aagaagtgac gatttttcaa catttttaaa aataaaaaat ataagcaacg attaattgta
      481 tatttagaat ggcttgtaat gtaatttatc tatgaaaatg aagcgagtta ttatgaccgt
      541 tatatgaatt gtgatacgct ttttaaaagt aatatgtatt tatgatttac cctgaaaaaa
      601 taaaaagaat caaaggagtc gatcattatg acagtattaa cagataaagt agcagtagtt
      661 acaggtgcag gtagtggtat tggagaagca attgcaacat tactacatga agagggtgtg
      721 aaagtcatct tagccggtag aaataaagat aaactacaaa atgtggcgaa tcaattagct
      781 caagatagtg tgaaggtagt gccaacagat gtaacgaaaa aagaagaagt cgatgaattg
      841 ataaaaattg cacaacaaac attcggtggt ttggatattg ttatcaatag tgcgggacaa
      901 atgttatcgt ctaagattac tgattatcaa gtagatgagt gggatagtat gattgatgtg
      961 aatatcaaag gcactttata tacggcacag gctgcattac caactatgtt agaacaatca
     1021 agtggtcatc ttattaacat tgcatctatt tctggctttg aagtaacgaa aagcagtacg
     1081 atttatagtg cgacgaaagc agcagttcac actattactc aaggattaga aaaagagttg
     1141 gcaaagacag gtgttaaagt aacaagcatt tctccaggaa tggtagatac agcaataact
     1201 gccgcataca atccaacaga tcgtaaaaaa cttgaaccac aagatattgc agaagcagta
     1261 ttatatgcat taacacaacc aaagcacgtt aacgtgaatg aaattacagt gcgtcctgta
     1321 taaaaaggtg tttgaggtct gggatatagg catttaatgt aatagctatc ggttttgtta
     1381 atattagtaa ggcaataatg atataagggc atgatagaca attattcctt gtacaattct
     1441 cagtaattgg tgattttaac aatcaagtga gtgaaggcga ctatattagt gttatgattt
     1501 ataatctata atgttcataa ttacagaact ataaaaactt tttcatttcc taccatattc
     1561 taggagttgc tttaatgacc aaaaaacatg tttttataat tattggtgtt attttatgta
     1621 tatgtatagt tgctacagtc atttatttaa aagtgaaata tgacgaaaaa gaaaagcaaa
     1681 aatcaattta ttataaagaa caacaagagc gtattacact ctatctcaag cataacacta
     1741 aagaaccgaa cacgatcaaa tctgtgcatt tcacaagttt aaagacagga cctatgggcg
     1801 atgccgtaat tgaaggttac atcaatgaaa ataaagaaga taattttgtt gcatatgggt
     1861 ctccagaaca taattatcaa tttggtggaa gtttaatcaa aagtaaaaat ttaagcacat
     1921 tattaaaacc agcacatcaa accaaaacgc ctgatgatat aaaaaaagaa ctagattcta
     1981 aaaagaacga ccgctaaaac aatgcggtcg ttttaatttt gctttcaagt taatcatact
     2041 tacacataaa aatgaaaagt tgaattttta gtacaatttt cactgtattt atctgttaac
     2101 cacttcataa ccgcaatatt ccgatgatct taaatgaaca cttttggctg cttttccttg
     2161 acctagatta aaattataga ttttctttgt aagagtgtgt tttgtagtat cataaatatg
     2221 taaactgtat aaaatcccac cacattttag gagctggtga tagatgtcga aaaagaaaat
     2281 tttaatatta actagtatta tgttaatcat attaattagt gttgcaagta tctattttaa
     2341 aatgaaatat gacgaaaaag aaaaacaaaa atcaatatac tataaagaac aacaagcgcg
     2401 cattacactt tatctcaagc ataacactaa agaaccgaac acgattaaat ctgtgcattt
     2461 cacaaaattg gaaacaagtc ctatgggaag tgctgtgatt gaaggttaca taaatgaaaa
     2521 caaaaaagat gattttgttg cctatgcatc acctgaaaat aactttcaat ttgtaggtga
     2581 tttaattaaa agtgaaagat taagtgagtt actaaaacca gcgcatcaat taaaatcacc
     2641 agatggtata aaaaaagaac taaataaaaa gaagagttac taaagttagg agttgcttta
     2701 atgtccaaaa aacatggttt tataattatt ggtgtcattt tatgtatatg tatagttgca
     2761 tccgtcattt atttaaaaat gaaatatgac gaaaaagaaa aacaaaaaga aatttattac
     2821 aaaggacaac aagagcgcat tacactttat ctcaagcata ataccaaaga acccaatacc
     2881 atcaaatctg tacatttcac aagtttaaaa agaggaccta tgggcgatgc agtaattgaa
     2941 ggttacatca atgaaaataa agaagctgat tttgttgcat atgggtcacc tgaagaaaac
     3001 tatcaattca ctggaggatt gacaggaagt aatgaagtct taggaaaatt aaagaacgct
     3061 gaaaatttaa aatctcctga aaaaattaaa gaagaaataa acaaaaagaa aaacactaaa
     3121 cagtgatttt cacgtaatat ttagcttgat ttgtacatgt ttaatatcgt ttgctatcga
     3181 aggtgtttcg caattaatat aaatcacttc atcatcacca atacttcccc agttttttac
     3241 agtacattaa cacaagcgaa ccccattaat gtaaatggaa taatagtttg gccattataa
     3301 gaacaatata tacctaataa caatggcgat ggcgtgattt attgacatta tcaaattttc
     3361 tcaaatgtgt gtgatttttt ataaaaaagg gtagagcaag gtataatatc ctttcaatct
     3421 gttttcatat tttatatatt ttttaaatat aagcgctaaa tgttttaact aaagcataga
     3481 ttgacaagat gttatacaga atttcaaatt ctatccaata ttgttcgaag tgtagtatca
     3541 ctggattggt attaaacaat gtaaaggaga gattgcaaat gccgtataat tacaagaaac
     3601 aaaatggaga gttaatgtct gtaatgagcc aaggtgaaaa gtttattcat caatcacccg
     3661 ttaatgatga acttagtgca ttgattaagt tattaatttc taaaattaac ggttgtcatt
     3721 attgtgttga tatccataaa aaagaattaa aggaattggg tgtaacacaa atgaaaattg
     3781 atgaagtctt gagttttaga catttagatt tatttactga tcaagaaaaa gtgacgcttg
     3841 aatttgcaga aatgttaaat tcaatcaaag actttaagaa gtttgaaatt attgaccggc
     3901 taaaatcatt ttatgatgaa gaacaaatta ttgatcttgt ctttgttgta aaccaaatta
     3961 acggttggaa cagactaaat attattagtg ataaactata attgttcata taaatacaga
     4021 gtttcatctc gagcgctata tcataacaaa tcatgccact atacaggtca aatcttgtat
     4081 agtggcattt taatttatcc ctttgaatac tgttatttaa cgaatatcgg tccacctggt
     4141 ccaacaggga tacctaatag gaaccaaata atgacaaata ctgtccacac aatacttagt
     4201 gcgattgaat acggcattaa actagaaagt aaggctccga gtttcatgcg tttatcgtat
     4261 ttttgtgcat aagttaataa taaaggtaag tacggcatca tcggtgtaat tggattggta
     4321 attgaatctc ctacacggta aatgacttgt gtgaatgcgg gatgaaagcc gataaggatt
     4381 aacattggta cgaatatcgg tcctaaaata ccccatttag ccgatgcgct tccgattaac
     4441 atgttgacca ttgcactcag tacgataata cctagtatca atacaatacc gttttgatgt
     4501 tctaataatt tggcaccttt aacagcagcg ataattccta aattactcca ctttaaatac
     4561 gcaagtagct gtgctgcaaa aaacacaata acgataaatg ttcccattga tcctacagca
     4621 tcgccgaaca ttttacctaa gtcttttgta tttttaattt ctttgcttaa aatcccataa
     4681 actaatccag gtactaaaaa tacgacaaga ataattaatc cgacaccgtt aattaatggc
     4741 gcatcgtcta gtaagctgcc tgttttagca tttcttaaaa agctatgttc aggaatggct
     4801 gtaataatta ataaaataat tgtgactatg aaactgatat ttgcccattt taaagcatgt
     4861 gcttctttgt cagtaatatg tgaagatgtt tcttcatggt catcatgcat taaactgtca
     4921 tcatattttc ctaatctagg aataatgagc ttagttgtaa ctagcaatat cgtaggaagt
     4981 aatacaacga cactcgctgc gataaagtac cagttcatag caacgtttgt tttaatagaa
     5041 tctgaaacga tacgtgttgc cggttctgta aatgaataga ccaaagcatc ttgcatacca
     5101 acaactatat ttgctgcaaa tcctccaaca gcggaagcat atgccatcgt taaaccagca
     5161 atagggtgat agccaatttt aataaaaagc attgctgcaa gcggcggcaa gataattgtc
     5221 gcagcatcgc cggctgtact acctaaaata ccaattaata taatagtcgg taaaattaaa
     5281 aatcgtggtg cgcgattcac aacagaaatc attaacttgt cgaagtatcc tgttttctct
     5341 gcaacaccaa taccaatcat cactgctagt actaagccta atgctgggaa ctctgagaaa
     5401 tttttaatcg tatcattcat tatcatcgta aatccatcat ggctaattat atttttaata
     5461 taaatggttt gatgtgtacc tggatgctta acagatacat taaataatga gataacccac
     5521 gtcataatgg ctaagcctat acacattaaa aagaataaga cgctaggatc tggcaattta
     5581 tttccaattt tttcaacact attcaagaaa cgattgacga tagacccctt ttgttgatgt
     5641 tttgatgtca tcaatgattc ccccctttgt taaatattta aagtgtaaca aaaaatactc
     5701 tcaaaagtaa caattttcag aaaatgaaaa gactaatatt gttaaatatt ttgagttatt
     5761 caatagaaat tgtatagcag agagtagcta agactgcctg gagacttatc tattaggttt
     5821 atgaagcatt gaacagtgga aaataaggac tgtaagttta agatatgttg tataggagtg
     5881 actgaatgaa acgtttggaa aataaagttg cagtcgtaac aggagcaagt acaggtatcg
     5941 gacaagcttc tgcaatcgct ttagctcaag aaggtgcgta tgtattggcg gtagacatag
     6001 ctgaagcggt atcggagact gtcgataaaa ttaaaagtaa tggtgacaat gcgaaggcgt
     6061 ataatgtgga tatttcagat gaacaacaag tggtagactt tgtgtctgac ataaaggaac
     6121 agtttggaag aatcgatgtg ttgtttaata atgccggtgt ggatgatgcg tctggtagaa
     6181 ttcatgagta tccaatagat gtgtatgaca agattatgaa tgtagatatg cgtgggacat
     6241 ttttaatgac gaaaatgatg ttacctttaa tgatgaatca aggtggctct attgttaata
     6301 cgtcttcatt ttcaggacag gcagcagatt tgtatcgctc aggatataat gctgcgaaag
     6361 gcgcagtgat taattttaca aaatcaatcg caattgagta tggtcgtgat ggcattcgat
     6421 ccaatgcaat tgcaccaggt acaattgaaa ccccgttagt agataaactg acaggtacga
     6481 gtgaggatga tgcaggtaaa acatttagag aaaatcaaaa atggatgact ccgctgggac
     6541 gtttaggtaa accagaagaa gtaggcaagc tcgttgtctt tttagcatct gatgacagtt
     6601 catttattac tggtgaaaca attcgcattg acggtggtgt tatggcttac acatggcctg
     6661 gcgagatgtt gagtgatgat tcgtggaagc ggacattgga ataattgttt taaaatcgat
     6721 atccagtgga aaataatttg gcatgaagta ggcaatgtat gcatattgtc gcaatgacag
     6781 acatttaaat cggagattaa ttaatctttg aaaatcacat attgttcatt tgaagtgtat
     6841 tgcttaaagc ttgttttaac gtattgtagg tcgtgcatct aatttatcag aatctcgagt
     6901 tcaaactttt ggagtatctt taaaataagc tgtattttgt ctattctaat aaattaagga
     6961 gaattttatg ttaaaagaaa aagaaagttt tagattgcta tatcaagcta taagagagat
     7021 tgcagataaa attggagata atcagttaga aactaattcc gttagtttat tattattgga
     7081 ctttgatttt gaacatgaag tatttgatga attgtatctt gcgattttaa aatatttaaa
     7141 tacagtaagt atagagaaca taagtcatag tgaacttttg aatttaatag aaaatacgat
     7201 tccagaagat cgagagatta acacgtttgt aaaaaataaa ataataattg gctttgctaa
     7261 taattatttc cctgaattac aagtattagc aaacgaaata aaatctgata tggctagttc
     7321 attaaaacaa tgatattttt atttaaattt ttaaagcttt gtacgaaatt gtacaaagct
     7381 ttttttggtg cgtattgtat gggcaacaac ttgacgatga aaatccgtta caggattggt
     7441 aataggaaat gttagcgaaa gacaagggta tccattgtag attaacaaaa ggacgtttcc
     7501 acaagtgtgg gttattctca ctaaagcaat acgcagagac aacttacgta aaattttgaa
     7561 ctgacgagaa cgaaacttct actcaattat tgataaaaat tttcaaaaag acttgaatgt
     7621 gctgagaata cgatgtttat ggaaggatta tcaaaatata aatgagcatt catttacaac
     7681 ctttattgac aatgattctc aactaatata gtatataatc aaatcgtaat agttacgatt
     7741 tgttttctgc aacttttttg aagttttagt tgaggtgaaa acagtaaaag catctaagtg
     7801 aatgtagtta acggacaact gcattcgctt gtagagccac aagaagcaac tttaaataag
     7861 gtttacggtt gcatgttgat gcaacaaccg attactaagt catgctttcc actttgcggg
     7921 ttagcatgac ttacctaata gatgggaaac tattaggttc accttctaaa aaattacagt
     7981 tatagaggaa tacagttgct tgcttcgcac caactgcata agagcctgag acataaatca
     8041 atgttctatg ctctacaaag ttataatggc agtagttgac tgaacgaaaa tgcgcttgta
     8101 acaagctttt ttcaattcta gtcaaccttg ccggggtggg acgacgaaat aaattttacg
     8161 aaaatatcat ttctgtccca ctccctaaaa atacatctaa aggagtgtcg tatgaatcgg
     8221 caggttatag aattttctaa gtataatcct tcggggaata tgacgatact tgttcattca
     8281 aaacatgatg ctagtgaata tgcatctatc gccaatcagt tgatggccgc aacacatgta
     8341 tgttgtgaac aggtaggctt tatagaatca acacaaaatg atgatggcaa tgattttcac
     8401 ttagttatga gcggtaatga attttgcggt aatgcgacga tgtcatatat acatcatttg
     8461 caggaaagtc atttgcttaa agaccaacag tttaaggtga aggtatctgg ctgttcggat
     8521 ttagtgcaat gcgcaattca tgattgccaa tactatgaag ttcaaatgcc acaagcccat
     8581 cgtgttgtac caacaacaat taatatgggt aatcattcat ggaaagcaat agaaattatt
     8641 tatgaaacat atgtacatta tgtgattcca gttaaacaag taacaacaga aattcaacat
     8701 ttggttgaat cgtatgttcg taagcaacaa tggaatcaca aatataaaac agtaggtatg
     8761 atgctttttg atgaacaacg tcaattttta cagccattaa tctatatacc agaaatccaa
     8821 agtttaattt gggaaaatag ctgtggttct ggtacagcat caattggggt ttttaataat
     8881 tatcaacgta atgacgcatg taaagatttt acagtacatc agccaggggg cagtatttta
     8941 gtgacatcaa agcgatgtca tcaattggga tatcaaactt caattaaagg acaggttaca
     9001 actgtagcta caggaaaagc atatatagaa taaggagcct acaatgaata actttaataa
     9061 tgaaatcaaa ttgatattac aacaatattt agaaaagttt gaagcgcatt acgagcgtgt
     9121 attacaagac gatcaatata tcgaagcatt agaaacattg atggatgact atagtgaatt
     9181 tattttaaat cctatttatg aacaacaatt taatgcttgg cgtgacgttg aagaaaaagc
     9241 acaattaata aaatcgctgc aatatattac agcgcagtgt gttaaacaag tggaagtcat
     9301 tagagcgaga cgtctattag acggacaggc gtctaccaca ggttactttg acaatataga
     9361 acattgtatt gatgaagagt ttggacaatg tagtatagct agcaatgaca aattattgtt
     9421 agttggttca ggtgcatatc caatgacgtt aattcaagta gcaaaagaaa caggtgcttc
     9481 agttatcggt attgatattg atccacaagc cgttgaccta ggacgtagaa tcgttaacgt
     9541 cttagcacca aatgaagata taacaattac ggatcaaaag gtatctgaac ttaaagatat
     9601 caaagatgtg acgcatatca tattcagctc gacaattcct ttaaagtaca gcattttaga
     9661 agaattatat gatttaacaa atgaaaatgt cgtagttgca atgcgctttg gtgatggcat
     9721 caaagcaata tttaattatc cgtcacaaga aacagcggaa gataagtggc aatgtgtgaa
     9781 taaacatatg agaccacagc aaatttttga tatagcactt tataaaaaag cagctataaa
     9841 ggtaggtatt acggatgtct aaattattaa tgataggcac tggtccggtc gcaatccaat
     9901 tagcgaatat ttgctattta aaatcagatt atgagattga tatggttgga cgtgcctcaa
     9961 catcagaaaa atcaaaacgc ttatatcaag cgtataaaaa agagaaacaa tttgaagtca
    10021 aaatacaaaa cgaggcgcat caacatctgg aaggtaattt tgaaattaat cgtttgtata
    10081 aagatgttaa aaacgttaag ggtgaatacg aaacggttgt catggcatgc acagcagatg
    10141 cttattatga cacactacag caattgtcgt tagaaacttt gcaaagtgtc aaacatgtca
    10201 ttttaatatc accgacattt ggttcgcaaa tgattgtcga acaatttatg tctaaattta
    10261 ataaagatat cgaagtgatt tcattctcaa cttatcttgg cgatacacgt attgttgata
    10321 aagaagcgcc taatcatgtg ttgacaacag gtgtaaaaaa gaaattgtac atgggatcga
    10381 cacattcaaa ctcaacaatg tgtcaacgaa tctctgcttt agctgagcaa ttgaagattc
    10441 aattagaagt cgttgaatcg ccactgcatg ctgaaacacg taacagttcg ctttatgtgc
    10501 acccaccact atttatgaat gacttttcat tgaaagccat tttcgaagga acggatgtac
    10561 cggtttatgt gtataagtta tttcctgaag gaccgataac gatgacacta atccgtgaaa
    10621 tgcgtttaat gtggaaggaa atgatggcta ttttacaagc atttagagtg ccgtcagtca
    10681 acctgattca atttatggtg aaggaaaatt atccagtacg tcctgaaact ttggatgaag
    10741 gtgatattga gcatttcgaa atcttgccag atatcttaca agaatatctg ctttatgtaa
    10801 gatataccgc aatcctcatt gatccatttt cacagccaga cgaaaacgga cattactttg
    10861 atttttcagc tgtaccattt aagcaagtct ataaaaatga acaggatgtt gttcaaattc
    10921 caagaatgcc aagtgaagat tattacagaa cggcgatgat tcagcatatt gggaaaatgc
    10981 taggtatcaa aacgccaatg attgatcagt tcctaactcg ctatgaagca agttgccagg
    11041 cgtacaagga tatgcatcaa gatcaacaac tatcttctca atttaataca aatctttttg
    11101 aaggagataa ggcactcgtc acaaaatttt tggaaatcaa tagaacgctt tcataataag
    11161 ggtttgaagt tttataatag aaaaaaatta ttgaattatg tttgacattt acataaaaat
    11221 aagcaaataa ttgagaaaaa taatcattac gatttgatta agtaatgcaa cttatcaatt
    11281 tagaaagagg aaaagcaaat gagaaaacta actaaaatga gtgcaatgtt acttgcatca
    11341 gggctaattt taactggttg tggcggtaat aaaggtttag aggagaaaaa agaaaacaag
    11401 caattaacgt atacgacagt taaagatatc ggtgatatga atccgcatgt ttacggtgga
    11461 tcgatgtctg ctgaaagtat gatatacgag ccgcttgtac gtaacacgaa agatgggatt
    11521 aagcctttac tatctaaaaa atgggatgtg tctgaagatg ggaagacata cacgttccat
    11581 ttgagagatg acgttaaatt ccatgatggt acgccatttg atgctgacgc agttaagaaa
    11641 aatattgacg cagttcaaga aaacaaaaaa ttgcattctt ggttaaagat ttcaacatta
    11701 attgacaatg ttaaagttaa agataagtac acggttgaat tgaatttgaa agaagcatat
    11761 caacctgcat tggctgaatt agcgatgcct cgtccatatg tatttgtgtc tccaaaagac
    11821 tttaaaaacg gcacaacaaa agatggcgtt aaaaagttcg atggtactgg tccatttaaa
    11881 ttaggtgaac acaaaaaaga tgagtctgca gactttaaca aaaatgatca atactggggc
    11941 gaaaagtcta aacttaacaa agtacaagca aaagtaatgc ctgctggtga aacagcattc
    12001 ctatcaatga aaaaaggtga aacgaacttt gccttcacag atgatagagg tacagatagc
    12061 ttagacaaag actctttaaa acaattgaaa gatacaggtg actatcaagt taagcgtagt
    12121 caacctatga atacgaaaat gttagttgtc aattctggta aaaaagataa cgcagtcagt
    12181 gacaaaacag tcagacaagc gattggtcat atggtaaaca gagataaaat tgccaaagaa
    12241 attttagatg gtcaagaaaa accagcaact caattatttg cgaaaaatgt aacagatatt
    12301 aatttcgata tgccaacacg taagtatgac cttaaaaaag cagaatcatt attagatgaa
    12361 gctggttgga agaaaggtaa agacagcgat gttcgtcaaa aagatggtaa aaaccttgaa
    12421 atggcaatgt actatgacaa aggttcttca agtcaaaaag aacaagcaga atacttacaa
    12481 gcagaattta agaaaatggg tattaagtta aacatcaatg gcgaaacatc agataaaatt
    12541 gctgaacgtc gtacttctgg tgattatgac ttaatgttca accaaacttg gggattattg
    12601 tacgatccac aaagtactat tgcagcattt aaagcgaaaa atggttatga aagtgcaaca
    12661 tcaggcattg agaacaaaga taaaatatac aacagcattg atgacgcatt taaaatccaa
    12721 aacggtaaag agcgttcaga cgcttataaa aacattttga aacaaattga tgatgaaggt
    12781 atctttatcc ctatttcaca cggtagtatg acagttgttg cgccgaaaga tttagaaaaa
    12841 gtatcattca cacaatcaca gtatgaatta ccattcaatg aaatgcagta taaataaagg
    12901 agcaattaga tgttcaaatt tatcttaaaa cgtattgcgc tcatgtttcc attagtgatt
    12961 gtagtaagtt ttatgacatt tctattgacg tatattacaa atgaaaatcc agctgtgaca
    13021 attttacatg cacaagggac gccaaatgta acaccagagt tgattgcaga aacgaatgag
    13081 aagtacggtt tcaatgatcc attattaatt caatataaaa attggttact tgaagcgatg
    13141 caatttaatt ttggtacaag ctacattaca ggtgatcctg ttgctgaacg cattggtcca
    13201 gcatttatga atacattgaa attaacaata atttcaagtg ttatggtgat gattacatca
    13261 attattttag gtgtagttag tgcattaaaa agaggaaagt tcactgatcg tgcgatacgt
    13321 tcagtggctt tctttctaac tgcattacca tcatattgga tagcttcaat acttattatt
    13381 tacgtttcag tgaagttaaa catattgccg acttctggat taacaggtcc agaaagttac
    13441 atattgccag tgatcgttat tacgattgcc tatgctggta tttactttag aaatgttaga
    13501 cgctcgatgg tggaacaatt aaatgaagat tatgtacttt atttaagagc aagcggtgtg
    13561 aaatctatca cattaatgtt gcatgtgttg cgtaatgctt tacaagttgc ggtatcaatc
    13621 ttttgtatgt ctataccaat gataatgggt ggactagttg ttatcgagta tatctttgca
    13681 tggcctggac taggtcaatt aagtttaaaa gcaatacttg aacatgattt tccagttatt
    13741 caagcatatg tattaattgt agcggtatta tttattatat ttaatacatt agcagatatc
    13801 attaatgcgc tattaaatcc aagattaagg gagggcgcac gatgataatt ttaaaacgat
    13861 tattacaaga taaaggtgca gtggttgctt taggcattat tgtattatat gtctttttag
    13921 gattagcagc accacttgtg acattttatg atcctaacca tatcgataca gcaaacaaat
    13981 ttgctggcat gagttttcaa catctactag gtactgacca tttaggtaga gatattttaa
    14041 ctaggttaat ttatgcgatt agaccaagtt tgttatatgt ctttgttgcg ctatttgttt
    14101 ctgtacttat tggatctatt ttaggattct tatcaggata tttccaaggg tttgttgacg
    14161 ccttaatcat gcgtgcgtgt gatgttatgt tggcattccc aagttatgtt gtaacgttag
    14221 cattaattgc attgtttggt atgggtgccg aaaatattat catggcattt attttgacgc
    14281 gttgggcatg gttctgtcgt gttatacgta caagtgttat gcagtacact gcttctgacc
    14341 atgtaagatt tgctaaaaca atcggtatga atgatatgaa aattattcac aaacatatta
    14401 tgccgttaac attagcagat attgctatta tctctagtag ctcgatgtgt tcaatgatct
    14461 tgcaaatatc tggcttttca tttttaggat taggtgtcaa agcgcctact gcagagtggg
    14521 gcatgatgct taacgaagct agaaaagtga tgtttacaca tcctgaaatg atgtttgcgc
    14581 caggtattgc catagtgatt atagtgatgg catttaactt cttatccgat gctttacaaa
    14641 ttgccattga tccccgcatc tcttctaaag ataaacttcg ttctgtgaaa aaaggagtgg
    14701 tgcaatcatg acattgttaa cagttaagca tttgacgatt acagatacct ggacagatca
    14761 accactcgtg agtgatgtga attttacatt aactaagggt gaaactttag gcgttattgg
    14821 agaaagtggt agtggtaaat caatcacttg taaatcgatt attggtttga atcccgaacg
    14881 actcggggtg acaggtgata taacctttga tggtaagcca atgttgtcgt tatccgaagc
    14941 acaattgaaa aaataccgtg gtaaagatat tgcgatggtc atgcaacaag gtagtcgtgc
    15001 atttgatcca tcgacaacag taggtaaaca aatgtttgag actatgaaag tacatacgtc
    15061 aatgtctaca caagaaattg aaaagacatt gattgaatat atggattatt taagtttgaa
    15121 agatcctaaa cgtatattaa aatcataccc ttacatgtta tcaggaggaa tgttacagcg
    15181 attgatgatt gctttagcgt tagctttgaa accaaagtta atcattgctg atgagccgac
    15241 aacggcttta gatacaatta cacaatatga tgtactggaa gcatttatag atattaaaaa
    15301 acactttgac tgtgcgatga ttttcatttc acatgattta acggttatta acaagattgc
    15361 agatcgtgtt gttgtgatga aaaatggtca actgattgag catggtacgc gtgaatcagt
    15421 cttgcatcat ccaaaacatg tttacacgaa gtatttattg tcaaccaaga agaagattaa
    15481 tgatcatttt aaacatgtga tgaggggtga tgtacatgat taaaattaaa gatgttgaaa
    15541 agtcatatca aagcgcacat gtttttaagc gtcgtcgaac acctatcgtg aaaggtgtgt
    15601 catttgagtg tccaatcggt gcgacgattg cgattatcgg agaaagtggt agcggtaaat
    15661 cgacgttgag tcgtatgata ttaggtattg agaaaccgga taaaggttgt gtaaccttaa
    15721 atgatcatcc gatgcataag aagaaagtga gacgtcatca aattggtgca gtgtttcaag
    15781 attatacgtc atcattacac ccatttcaga ctgttagaga aatcttattt gaagtgatgt
    15841 gtcaatgtga tggacaacct aaagatgtta tggaagtcca agcaattaca ttgttggaag
    15901 aagtcggtct atctaaggca tacatggata aatatcctaa tatgttatca ggtggagagg
    15961 cgcaacgtgt tgcgattgcg cgtgcaatat gtattaaccc taaatatatt ttgtttgatg
    16021 aagccattag ttcactcgac atgtcaattc aaacacaaat attagattta ttgattcatt
    16081 tacgtgaaac gcgtcagttg agttatattt ttatcacaca tgatattcaa gctgccacgt
    16141 atttatgtga tcaattaatt atttttaaaa acggaaaaat agaagaacaa attccgacaa
    16201 gcgcattgca taaaagtgac aatgcttata caagagaatt aatagaaaaa caactatcat
    16261 tctaaggagt gagataatga aaggtgcaat ggcttggccc tttttgagat tatatatatt
    16321 aacattgatg ttctttagtg ccaatgcaat cttaaacgtg tttatacctt tgcgaggtca
    16381 tgatttaggc gcaacgaata cggttatcgg tatcgttatg ggggcataca tgttaacagc
    16441 aatggtattt cgaccatggg caggacaaat tattgctcgt gtcggtccca ttaaagtatt
    16501 aagaattatt ttgattatca atgccatagc tttaattatt tatggtttta ctggcttaga
    16561 aggttatttc gtagcacgtg ttatgcaagg tgtgtgtacg gcattctttt ctatgtcttt
    16621 acagctaggt attattgatg cattaccaga ggaacatcgt tctgaaggtg tatcattgta
    16681 ctcgctattt tcaacgattc caaacttaat cggcccatta gttgccgtag gtatttggaa
    16741 tgcaaataat atttcactat ttgcaattgt cattatcttt atcgcattaa caacaacatt
    16801 ctttggctat cgcgtgacct ttgctgaaca agaacccgat acgtcagata agattgaaaa
    16861 aatgccattt aacgctgtaa ctgtttttgc gcaatttttc aaaaataaag agttgttgaa
    16921 cagtggtatt atcatgattg ttgcatcgat tgtatttggt gcagttagta catttgtacc
    16981 gttatacaca gtgagtttag gattcgcgaa tgcgggaatc tttttgacaa tacaggccat
    17041 cgcagttgtt gcggcaagat tttacttaag gaaatacatt ccgtcagatg gtatgtggca
    17101 tcctaaatat atggtatctg tactatcatt attagtgatc gcgtcatttg tagtggcatt
    17161 tggtccgcaa gtaggtgcaa ttattttcta tggtagtgcg atattaatag gaatgacgca
    17221 agcaatggtg tacccaacat taacatcata cttaagtttc gtcttaccaa aagtaggtcg
    17281 taatatgttg ttaggtttat ttattgcctg tgcagactta ggtatatcgt taggtggcgc
    17341 attgatggga cctatttccg atttagtagg atttaaatgg atgtatctaa tttgtggtat
    17401 gttagtcatt gtaataatga ttatgagttt attgaaaaaa ccaacaccac gcccagcaaa
    17461 tagtctttaa tgaagtgaat taaagcatat taagttaatg aatatttaaa ttttaaaagg
    17521 tatattgagc atggcgattc atgggagtgg gacagaaatg atattttcga aaaatttatt
    17581 tcgtcgtccc accccaactt gcattgtctg tggaaattgg gaatccaatt tctctttgtt
    17641 ggggcccaca ccccaacttg cattgtctgt agaaattggg aatccaattt ctctttgttg
    17701 gggcccctga ctagaattga aaaaagcttg ttacaagcgc attttcgttc agtcaactac
    17761 tgccaatata actttgtaga gcattgaaca ttgatttatg tctcaggctc tctatatggc
    17821 tcgtttttag aacgacagta tatctaaata aagcacgctt agaagtgagt tgcgatactg
    17881 acttttaagt gtgctttttg atgtgtatgg ataatggata atgctctgaa aaattttaca
    17941 tgaaaatatg caaaacgagt ataactgcta attgatagaa atagctcacc ataaaattac
    18001 ggtttgattt taaatataag taagtcgcac tactagctag tatcaatgct ggaatgaatt
    18061 cccaccatgt attaatgtat ggataatagt acagtgtttc aaggataacg gataatacta
    18121 ttgtaatctt taaaggtatt aatctgctta attcttgaat taaaatatga cggaaaataa
    18181 gttgacaaat caaagtattt aatataatgg ttaacgaaaa tatagctatt aaactgatgg
    18241 aaccatattc tctaatgagc gggtaaatgt caaagacagt aaaggaatct acatttagtg
    18301 cgaaaatttc gaaatgattt aaaagtaaaa agagtacgat acttaatgta aatgatataa
    18361 gaatatgcca tttatatttg gtgctggcaa cgatttgcga acgtatcatt ggaataaacg
    18421 catcttcatg catccgacga aaaatagcta atgaaagaat aactgcgagt aaatagctaa
    18481 tgttcattga aataggaaaa gagaaatccc acggagcttg ttgagtgaat atagctacta
    18541 acccaaaagt taaaaaaacg ataatgatcg gcaagatgtt aaccaaaaat atgtaaagga
    18601 aaataaatcc aatatcacgt ttgaaaaaac gcgattgttc ggtagcgtat tcttcttcta
    18661 tgtaatgttt atttgtattt gacatagtat acctcttaaa tagttgtatt atatagatac
    18721 tttagcacat attactttgt attgtatgtt ttatacatta aaatttaaaa tgaaaaacat
    18781 atcataaaat tgttttataa aatgaagcgc ttccattgtg ttttattttg taaggtgtat
    18841 cataaatatt gaattgaaat tttgggggga ggtattgtaa tgacgtttct tacagtcatg
    18901 caatttatag ttaatattat cattataggc ttcctgctta cggttattgt tatcgggctt
    18961 atttggttaa ttaaagataa aagacaatca caacatagtg tattaaggaa ttatccttta
    19021 ctagcacgta ttagatatat ttcagaaaaa atggggccgg aattacgtca gtatttattt
    19081 tctggggata atgaagggaa acctttttca cgtaatgatt ataaaaatat cgttttggct
    19141 ggaaaatata actctcgtat gaccagcttc ggtactacta aagattatca agacggcttt
    19201 tacatacaga acacaatgtt tccgatgcaa cgtaatgaga tttcagtaga taatacaaca
    19261 ttgttatcaa cattcattta taaaatcgcg aatgagcgtt tatttagtcg tgaagaatat
    19321 cgtgtaccga caaagattga tccgtattac ttaagtgatg atcatgcaat aaaattaggt
    19381 gaacatttaa aatatccatt tattttaaaa cgtatcgtag gacaatctgg tatgagttat
    19441 ggagccttag gaaaaaatgc cattacagct ttatctaaag gtctagctaa agcgggaact
    19501 tggatgaata caggtgaagg tggcttatca gaatatcatt taaaaggtaa tggggatatc
    19561 attttccaaa ttggtcccgg tttatttggt gttcgtgata aagaaggtaa ttttagtgaa
    19621 ggtttattta aagaggttgc acagttatct aacgtacgcg catttgagct gaagttggca
    19681 caaggtgcta agactcgtgg tggtcatatg gaagctgaaa aggtaaacga agaaattgct
    19741 aaaatccgaa atgttgaacc ttataaaaca atcaattcac ctaaccgtta cgaatttatt
    19801 cataatgctg aagatttgat tcgtttcgtc gatcagttgc agcaattagg tcaaaaacca
    19861 gtaggattca aaattgtagt aagcaaagtt tcagaaattg aaacacttgt acgtacgatg
    19921 gtggaattag ataagtatcc aagctttatt acgattgatg gtggtgaagg tggtactggt
    19981 gcaacattcc aagaattaca agatggtgtt ggcttaccgc tatttacagc tctacctatt
    20041 gtgtctggca tgttagaaaa atatggtatt cgtgataaag taaaattggc ggcatctggt
    20101 aagttagtga cacctgataa aattgcgatt gcactaggtt taggtgcaga ttttgttaat
    20161 attgcacgtg gtatgatgat tagtgtcggt tgtattatga gtcaacaatg tcatatgaat
    20221 acatgtcctg taggtgttgc aacgacagat gcgaaaaaag aaaaagcatt gattgttgga
    20281 gaaaagcaat atcgtgtcac aaactatgta acaagtttgc atgaaggctt attcaatatt
    20341 gcagcagctg ttggcgtatc cagtcctaca gaaattactg ctgatcatat tgtatatcga
    20401 aaaatcgatg gtgaattgca aacgatacat gattataaat taaaactcat tagttaactt
    20461 atttatttcg ggaaaatgaa agcagcggat tttagcgtta ctgcaaataa ttttatatta
    20521 gtagtggatg ctggtcacag aagaatttca aatattaaag ccctcagaat atgaattaag
    20581 gtttgtaacc ttagtcttat ctgagggcat ttttaagtta taaactattt atcgtccatt
    20641 ttatcttttt cttttaaacc tctgtgcttt aattgctttt caagtttttc aaaactaata
    20701 tctttatttt ctttagttga aacaccaaga cgtttattta actttttcat gtcaacttct
    20761 gtgtaatcta tgtctaagtg ttcaattgct tttttatctt tatagtctac tttgtatttt
    20821 acgcctttaa ggtctttgaa aatactttca gatttggcga ataacttttt ggcttcgtct
    20881 ttatccatac ctagatcgtc atatttaatt gtgttgattg tagactgttt taaaacttta
    20941 tcatttttat atgtgataga agttagtaca tgtttaccac taacatcacc ttcatatgtt
    21001 ttggtttgtt ctttaccaca agctgataat gcaatgatac aaactaatgc tactacaatt
    21061 aatgaacata attttttcaa agtcagtcgc cttctttcga tatttgtatt ataaagaaat
    21121 tataacattt actaaaaaat gatgttattc aaaaatttaa attttgtcat tttttttgga
    21181 gatatgaggt ttttgaagcg gatttctcac aaagttttta aaattattaa gtctgaaaat
    21241 gataaagcgg tagggaacgt agtgtgttcc actgctgatt tctttttgag cattatagac
    21301 ggtatgttgg aagaaaagaa caaaagcttg tgtacgatag catataggta ttaagaatac
    21361 gatgaactcg ttggtaatat tgacgtactg aagtgcgtac gctataatta gagtaacaga
    21421 agacaaagga gggtttatat gattatcact agccctacag aagcgagaaa agatttttat
    21481 caattactaa aaaatgttaa taataatcac gaaccaattt atattagtgg caataatgcc
    21541 gaaaataatg ctgtgattat aggtttagaa gattggaaaa gtatacaaga gacaatatat
    21601 cttgaatcta ctggtacaat ggacaaagta agagaaagag aaaaagataa tagtggtaca
    21661 acaaatatag atgatattga ttgggataat ctttaatgag caattacacg gttaagatta
    21721 aaaattcagc gaaatcagat ttaaagaaaa taaaacattc ttatttaaag aagtcatttt
    21781 tagaaattgt tgagacttta aaaaatgatc cgtataaaat aacacaatct tttgaaaaat
    21841 tagagcctaa atatttagag cgatattcaa gaagaattaa ccatcagcac agggtcgtct
    21901 ataccgtaga tgatcgaaat aaagaagtat taatactatc ggcatggtca cattatgatt
    21961 aatgaatatt caatatctga ataactttaa tgataagtta attaaagaaa ctagtattta
    22021 agtgtaggga aaatagcgac gttaatgcgt tgttatctct acacttttta attttataat
    22081 agcgcaagac taaaaagatt gaaattagta acaataaaag aataacgtat tataataagg
    22141 aattttaaat tctgactttt tcggaatatt aaattttaga aatatgaggt ttttaagcgg
    22201 attcctcaca aaattttaaa aatatttaag cctgaaaatg ataaagcggt agggaacgtt
    22261 tttctgaaag ttagtgatac aatagtttta agttgaaata caggaggatg aataacatga
    22321 atcagtcagt caaattactt aaacatttaa cagatgtaaa cggcattgct ggttatgaaa
    22381 tgcaagttaa agaagcaatg cgtaactata tagagcctgt cagtgatcaa attattgaag
    22441 ataacttggg tggcattttt ggaaagaaaa atgctgagaa tggtcaatac tcaattatga
    22501 tttctggtca tatggatgaa gttggtttta tggtaacaaa gattgataaa catggtttta
    22561 tttcatttac gccagttggt ggatggtgga atcaagtcat gctatctcaa aaagtaacga
    22621 ttacaacaga ttcgggcaaa gaaattagag gtatcatcgg ttctaaaccg ccacatgtct
    22681 taacgcctga agaacgtaaa aagccaatgg aaatcaaaaa tatgtttata gatattggtg
    22741 ttagtagcaa gggagaagct gaagaagctg gcgttgaagt aggcaatatg gttacgccat
    22801 atagtgaatt tgaagtgctt gcaaataata aatatttaac tgcgaaagca tttgataatc
    22861 gctatggctg tgcattagct attgaggtat tacaacgttt aaaagataaa aatattggca
    22921 tcaacttata cagtggtgcc acagtgcaag aagaagttgg tttgcgtggt gctaaagtgg
    22981 cagcgaatac gattaaacca gacttggcga tagccgtcga tgtaggtatt gcttatgata
    23041 ccccaggtat gtcaggtcaa acgagcgata gtaaactagg cggtggtcca gttgttatta
    23101 tgatggatgc tacaagtatt gcgcaccaag gtttgcgtaa gcatattaaa gatgtagcta
    23161 agaaacataa catcgaagta caatgggata cgacaccagg tggaggtaca gatgcgggaa
    23221 gtattcatgt cgcaaatgaa ggtattccaa cgatgacaat aggtgtcacg ttacgatata
    23281 tgcactctaa tgtatcggta ctcaatgtgg atgattatga aaattctatc cgtcttgtta
    23341 ctgaaattgt ccgttcattg aatgatgaaa cttataaaaa tatcatgtgg tagtcaaatc
    23401 cataaataat aaagaaagtc tttaatatga tgtcatgcaa gaactaagtt agatgcttag
    23461 ttagcatgag atgtcatgtt aaaggctttt ttattttcaa aaagtgagaa taagtcactg
    23521 cagtgtactt caattttgac ataaatgtta aaaaaatttc ccggaatgaa atggcactaa
    23581 atttttcaaa aatgggaata ctatatttga tatgtcttat tagagcgatt ttaaggggta
    23641 ataagcattg actaggtcga cttataggta agcacactat tcaagaaaag gaatacccta
    23701 ttatatcgaa ataactcatg atacgaggag gggtaaatat gttgttagag ataaaagatt
    23761 tagtatatga ggcaagtgat agagtcatac taaatcatat taatttaaaa gtagataaag
    23821 gtgaaaccat tgcgattatt ggtccttcag gtagtgggaa aagtacattt caaaaaataa
    23881 tatgcaattt aattagccca accagtggaa cactttactt taaagataaa ccatatgatg
    23941 attacgaacc agaaatatta cgccgacaca ttagctattt gatgcaacaa agcgatttgt
    24001 ttggtgaaac gattgaagat aatatgattt ttccgtctct tgcgcgtaat gataaatttg
    24061 ataagaaacg tgctaagcaa ttaattaaag atgtaggttt aggacactat caattaagtt
    24121 ctaaaataga acatatgtca ggtggcgagc gtcaaaggat cgcgattgca cgccaattaa
    24181 tgtatacccc agacatcctt ctattagatg aatcaacaag tgcgttagat attcataata
    24241 aagaaaaaat agaaaatatc atttttaaat tagcagatca aggcgtggca attatgtgga
    24301 ttacccacag cgatgaccaa agtatgcgac actttcaaaa gcgtataaca attgttgatg
    24361 gtcaaatttc taatatagag gagttgaacc aacatgagta atacggcatt aggactgacg
    24421 gcactgctat tagttattcc aattattatt tcttataaag aaggtctaca tattattaaa
    24481 gatttgattg tggcgacact acgtgcggtc gtacaattaa tcattttagg attcttactg
    24541 cactatattt ttaaaattaa tgacaaatgg ctattagtat tatgtgtatt tgtcattatt
    24601 gttaatgctt cttggaatac gattagtcga tcatcacctg tcatgcatca tgtcttttgg
    24661 atttcctttg tggctatctt tgttggaacg gcattaccgc ttgcaggtac tattgcgaca
    24721 ggggccatcc aatttacagc caatgaagtt atacctatcg gcggcatgct tgcaaataat
    24781 ggcttgattg caattaattt agcttaccag aatttagatc gtgcattcgt acaagatggt
    24841 actaatattg aatctaaatt atcacttgca gctacaccta aattggcttc taaaggtgca
    24901 atacgtgaaa gtattcgttt agctatagtg ccaacaattg attcggttaa aacatatggg
    24961 cttgtgtcga ttcctggtat gatgacaggc ttaattattg gtggcgtacc acctttacaa
    25021 gcgattaaat ttcaattgtt agtcgtgttt attcatacaa ctgcgaccat tatgtctgct
    25081 ttgattgcga catatttaag ctatggtcaa tttttcaatg caagacatca attagtagca
    25141 cgaaatacgg atgttaagag tgagtcatga tagattttac cgcatcatat ttaggcatta
    25201 gttttaattg gaaataaagt gatgcacata tagtaatgtt attatttagc gcagcgaaaa
    25261 tattcataaa ggcacgcata ctttgtagtc agttatctgt tctgacatat aaagcgtgcg
    25321 tgcttttttg gagtcattgt tgaaactgaa gtaattatac ataattatta aatgacatac
    25381 ttgtgttaat ttttcaaata ctgaaaaaca atttcaataa ttttccaatt aagcacagaa
    25441 aattaaagca aaatattata taatagaacg gttatatata aaattgtgtt gcacacattt
    25501 tttaataaat cgttattcta agggaaatga atatcggaaa ttttgtttga aaggagtttt
    25561 aaaatgtcaa tcatgcgact atttacattc attttaagta tttttatcgt aggaatggtt
    25621 gaaatgatgg ttgcaggaat tatgaacttg atgagtcagg acttacatgt atcagaagct
    25681 gtcgttggtc aattagtgac aatgtacgct ttaacatttg cgatatgtgg acctattctg
    25741 gttaaattaa cgaaccgttt ttcatcaagg cctgtattat tatggacatt acttatattt
    25801 atcattggta atggcattat tgctgtagcg ccaaattttt caatattagt agttggtaga
    25861 attatctcat ctgcagcagc agcactaatt attgtgaaag tattagctat tacagcgatg
    25921 ttatcagcac ctaaaaatcg tggcaaaatg attggacttg tctatacagg atttagtggt
    25981 gccaatgttt ttggtgtacc aattggaacg gttatcggcg atttagtagg ttggcgctat
    26041 acatttctat tcttaattat tgtgagtatt attgttggct tcttgatgat gatctattta
    26101 ccgaaggatc aggaaataca acgaggctct gtgaatcatg aggcaccatc tcatgaaaat
    26161 catgttactt cgaaaatatt aagacctgct gaagtagcaa aatatttaat tattacgttt
    26221 ttagtattga ttgcaaactc agtgacattc gtctttataa atccacttat tttatctaat
    26281 ggtcatgata tgtcattcgt ttcattagca cttctagtaa atggaatcgc tggcgttatt
    26341 ggaacatcat taggtggtat attctccgat aaaattacaa gtaagcgttg gttaatgatt
    26401 tctgtttcaa tttttatcgt gatgatgtta cttatgaatt taatcttacc tggttcaggt
    26461 ctattgttag caggactatt tatttggaat atcatgcaat ggagtactaa tccagcagtg
    26521 caaagcggtg tgattcaaca tgttgaaggc gacacaagcc aagtaatgag ttggaacatg
    26581 tctagtttaa acgctggtat tggtgttgga ggcattattg gaggcttggt catgacacat
    26641 gtttctgttc aagctatcac gtatacgagt gccatcattg gcgcattagg attaatcgtt
    26701 gttttaacat tgaaaaataa tcattatgct aaaacattta aatcatcata attctcatat
    26761 gaaaagcacg cctgctatca aattcaggtg tgctttttta gatgcaataa cgttattgat
    26821 atgtgcgata atagcgacgt tcattatgat atatcggcca aggcatttta ccgcttttag
    26881 caaaattagc taaatcattt tgcatttgtc gacttaaaaa tttaagatga tcagtgattg
    26941 gatactgatg cgctgataaa atttgtaagt ttccgaacca aaatatcata tcaagaatat
    27001 ggtacgcact gcgatagtgt gcgctagaag tatcatgcca tgcaaattca gcaagccata
    27061 gttgtgcgtt tgaatcttgt atattaagtt gttgtaaaaa ttgcttcatc ggctgtttga
    27121 aataacaatg tgtaatcgct aaagattgtt gcttcgccgt ctgaacatct tcgtatttta
    27181 aaggaatatc atttaattcc ataatgtcaa taaagcgttg cggtgataat tttttctgtt
    27241 cactagtaat ataaatatcg ccttcatctt ttgtataaca tgcaaaaatt ggtttcgttg
    27301 ttggataatt attttgtata taatctgttt taataggcgc atatattaaa tcaagccctt
    27361 tagaaggtcc tcgagattgt tttagcttcg ccatcagcat aagaatatca tctgtcgata
    27421 atgatgtaac atcatctgta tctatataat cgagcatcat tttttggaaa tgttgtgctt
    27481 tattgcgtgc actctcaagg gtgtctaatc gtagtgcgcc acttagtaga acgactttat
    27541 ggaagtatgg ctcaatgtca ggtattttaa gtaaagtcaa aatgctcata ctgcctgcag
    27601 actgacccat taaagtaatg ttattagcgt cgccaccgaa ggattcaata aattgatgca
    27661 cccattttat gacattgatt tgatctgaaa gtccattatt ggaatgaaac cctttattaa
    27721 aatatgacca gtctaaatat cctaatgcgc ctaaacgata attgcatgta ataacaataa
    27781 tgtcgttatt ttgtactaaa tgtgccggtt gatagagttc ggctgtacca tgaccatttt
    27841 caaaactacc accataaaaa taaatgatga caggtttctt cgactgatca ttatgttgtt
    27901 tccaaatatt tagatataaa cagtcttcat gttcggtaaa agttgcagat tgtgaggaga
    27961 aaaaatcttc taatttgttg tctggttgcg gtgggatggg ttgtatttca gttgcatcaa
    28021 ttggctctga ccattgtgtt tttaacgtag aatgtttaaa gcgattgtca tgaactggtg
    28081 gttctgcata aggaatgcct aagaagatat ctaaacgatc ttgtgtaata ccataaattt
    28141 gaccacctgt agtattaatc ttcatattta ttaacctcct ttatcatccc caatttaatc
    28201 ataccacggt ctatagatga taggtgacaa gtataacaag acaattttgt gactgaatca
    28261 gacgagcttg atatctgttt taactcaaac cattttcagc attatatgat gttcttctac
    28321 agtgacattt cattacaata tcactctcgc catcttgtta tgttagttat ttataaccga
    28381 ttttcaaaat aaaaaaccaa ccaagatgtt gaaatcaatg gttggttaat gtgtgtgttg
    28441 aaatcatata tggcactgca ttttaaaata tttaatgaga tgactttgat tcattattta
    28501 gtgactcatg catcttcgta ttgattcgtg ttgcgcgacg gaaatagacg ctttcaaatg
    28561 aactcgttgt accgatgtaa tagaagataa gcgccacaat aataataacg ataaagtcaa
    28621 atggatagtg aatcatattt aagcctttaa attctttgct tcctataaat gacagtattg
    28681 atagcacgat taaatataca ataatccata agctaccacc aatttgtttc tttgtattac
    28741 gccaattcat acgatattca tagaagaagt agattggtaa tccaagtata atgattaaaa
    28801 taacttcagc cgttgttggc cacattgccc aatatatagc taatgaagct aatacaaatg
    28861 ataatggtgc cattactttt aaaatttttg ctctaaatgg acgagtcatt gttggtccca
    28921 tttttcttaa tgcaatcact gtcgttgggc cagttaaata ggctactaaa gttgcagtag
    28981 aaataactgc tgctagcgta ccccaatctc taaataatgt aaccataatc atactaatga
    29041 tggcattaaa gatgattgct acacgtggga tatgatattt ttcattcatc ttcccaagaa
    29101 atttagggat atgtccattt ttctccattg ctcgtaaaac tcgacctgta acggcgacaa
    29161 atgacacgcc agtaccgaat ggtgatacaa aagcttcaat atatagtaaa attgcgagcc
    29221 aattaattcc taataagata gctaaatcag caaatggtga attgaagttg atgccattcc
    29281 atccactatg ttgtaacatt gattgaggca tagaagtgat aaacgtactt tgtaaaatga
    29341 tgtacaacac tgcactgatt gacagtgaga tagcgatgcc tcttgcgata tttttttcag
    29401 gatttttaat ttctgaaccc atattaataa ttgtctggaa tgaattgaat gaaaaaataa
    29461 tccctgatgc tgttgttgca gcaaaaatcg gtgcacttcc gtaaggcata aatgtgcttg
    29521 ccgaatggcc gtaatttgaa gtgtcgaatc ctgataacat caacatgatg atggttaaca
    29581 tgggtacgcc taatttaaat acagaaatta aactcgtaaa tgatgttaaa agttttacgg
    29641 accaatagtt taataatgaa aaaataacaa tgatgagata tacagctagc aatccgtatg
    29701 tgctaataga gccattttcc attaaatatc tcattggttt cgcccaatgc cacggccaag
    29761 aactcatata ttgaacagct gacacagctt cgataggtat tattgtcacc aaagacaccc
    29821 aattcgccca agcagcaata aagcctaata atgagccatg tgtatactgg gcatagttac
    29881 tcatgccacc tgattgagga aacattgtgc cgatttcaat gtagttatag gcaatggttc
    29941 caatgactag gaatccaaga acccatgata tgattgctgc tggtccagct attgatgatg
    30001 cttcccacgc accaaatagc caaccagatc ctattaatga acctaaccct agtaatacta
    30061 attgggatag attgattttg tcgttgtgca attgcttgtc tttattcata aatactcctt
    30121 cgttattgcc gaaaattcaa aaaataatat gcgaaaaatt atacgcttat tataataata
    30181 gtcaaatgca ttttttgtaa gaacgcaaaa gcatttgtaa aattagtttt ttataaaatt
    30241 aggatatata atacaatgtg tattgaaaaa caaaatggaa gtgatgacta tgagtgagga
    30301 acaatcaaat gaacaagttg aagcattttt aaataaagag agtcaatggc aagaagaata
    30361 taaatatttg agagcgttaa tctttaatga aacagaatta gaggaagcgt ataaatggat
    30421 gcatccttgt tacacgttga ataataaaaa tgtagtactt atccatggct tcaaaaatta
    30481 tgttgcacta ttatttcata aaggtgccat tttggaggat aaatatcata cactcattca
    30541 acagactgaa aaggtgcaag cagctcgtca gttacgattt gaaaatttaa cagagattca
    30601 agcacgtagc gaagaaatta aatattatct agccgaagca attaaagctg aaaaagctgg
    30661 taaaaaagtt gaaatgaaga aaacagagga atatgttatt cctaaagagt tagaagctaa
    30721 gttcgaagaa atgccacagc tagagtcgtc attttataaa ttaacgccag gcagacaaca
    30781 tcaatatata tatcatattg ggcaagctaa acgcagtgaa acaagacaaa agcgtgttga
    30841 aaagtatatt aaccaaatac tagaaggtaa agggatgcat gataagtaat taatgagtaa
    30901 aagcataccg gttatacaac aacatacaag atgacacgaa acaaccaatg gctcatgctg
    30961 ttggttgttt ttttaggtgt gctgtcatgg gcaacacttt gacgttggaa ttccgttaca
    31021 ggcttgggag taggaactgt tagcaaaagg taatggtgtc tacaatgaat gatgaagata
    31081 ttaaaatata aggatgactt tgtgggtggc ggatgggctt ttgtccgcct gtaacaatgg
    31141 atgcgtgtgc attattacaa aaattcgact tttgtaataa tatttcacat tttcgacact
    31201 tttttgctat aaaacaacca attgagcgat aataaattcg cttttaaaaa atatgagtta
    31261 tctatttagt tgccaaagat aaaataataa tgtttaataa catcatatag agtatgttag
    31321 ttttaaatgt cgaatatacg aatgtgcaaa caaagtaatc ggtagaaatt caacatacat
    31381 agcgccgttt actgttaagt attcacatta cagatgaaaa atataaaatt ctacataatc
    31441 aagaccatga tgtgtacttg tttaacttat gactctattt gtttaacaat tgcgatgatg
    31501 gtctttttat tttatgcgta tcattcgtca tattttttat gaggaaggag aaatgattat
    31561 gttaagtatt aagcatttaa caaaaattta ttctggtaat aaaaaggcag tagatgacat
    31621 ctctttagat attcaatctg gggaatttat cgcatttatt ggaaccagtg gaagtggtaa
    31681 aacaactgct ttaagaatga taaaccgtat gattgaagcg acagaaggac aaattgaaat
    31741 tgatggcaaa gacgttcgga gtatgaatcc tgtcgaatta cgtagaaata ttggctatgt
    31801 tattcaacaa attggcttaa tgcctcatat gacgattaaa gagaatattg tgttggtacc
    31861 caagttgttg aaatggacta aagaagaaaa ggataaacgt gcaaaggaat taattaaact
    31921 tgtggattta ccggagtcat ttttagagcg ttatccagca gaactatcag gtgggcaaca
    31981 acaacgtatc ggtgtcgtaa gagcacttgc ggccgaacaa gatattattt taatggatga
    32041 accttttggt gcattggatc ctattacgag agatacgtta caagatttag ttaaaacgtt
    32101 acaacgaaaa ttaggcaaga cgtttatctt tgtaacacat gatatggatg aagcgattaa
    32161 attagcagac aaaatttgta ttatgtcaga aggtaaggtg gtgcaatttg atacgccaga
    32221 caatatttta agacatcccg caaatgattt tgtacgtgat tttataggac aaaatagact
    32281 gattcaagac cgtcccaatg acaagactgt agaaggtgta atgattaaac caatcacgat
    32341 acaagcagaa gcaacactga atgacgccgt tcatattatg agacaaaaac gtgttgatac
    32401 tatttttgta gtagatagta ataaccattt actaggtttc ttagacattg aagatataaa
    32461 tcagggtata cgtggacaca aaagtttacg agacaccatg caacaacata tttacaccgt
    32521 tcaaattgat tctaaattac aagattctgt acgtacgatt ttaaaaagaa acgttaggaa
    32581 tgtacctgtc gtagatgata agcaacgttt agtaggactg attacgcgtg ccaatgttgt
    32641 tgatattgta tatgacacga tttggggcga tagtgaggat gcagtgcaaa cagaacatgt
    32701 gggggaagac actgcgtcct caaaaatgca tgagcaacac actactaatg tcaaagtacg
    32761 tgacatagga gatgataaat catgatcgag ttcctacatg aacatggtgg acagttgatg
    32821 tcgaaaacac tggaacattt ctatatttct atagtggcat tattacttgc catcattgtt
    32881 gcagtaccta taggcatttt attatcaaaa acaaagcgaa ctgccaatat tatattaact
    32941 gtggcaggtg tcttacaaac gattccaaca ctagctgtac ttgctattat gataccgatt
    33001 tttggtgttg gtaaaacgcc tgcaattgta gcgctattta tttatgtgtt attacctatt
    33061 ttaaataaca cggtactcgg cgttcaaaat attgatagca acattaaaga agctggaaaa
    33121 agtatgggaa tgacacaatt tcaattgatg aaggatgttg aattgccgtt agcattgccg
    33181 cttatcattg gtggcattcg tttgtcatct gtgtatgtaa ttagttgggc tacacttgca
    33241 agttatgtag gtgcgggtgg attaggtgat ttcattttca atggtttaaa tttatatgat
    33301 ccactgatga ttgtaactgc aacggtactc gttactgcac tagcattagg tgttgatgcc
    33361 ttattagctt tagttgaaaa atgggtagtt cccaaaggct taaaagtatc tggataatta
    33421 ggaggctaag ataatgaaga aaattaaata tatacttgtc gtgtttgtct tatcgcttac
    33481 cgtattatct ggatgtagtt tgcccggact aggtagtaag agcacgaaaa atgatgtcaa
    33541 aattacagca ttatcaacaa gcgaatcgca aattatttca catatgttac ggttgttaat
    33601 agagcatgat acacatggta agataaagcc aacattagta aataatttag ggtcaagtac
    33661 gattcaacat aatgccttaa ttaatgggga tgctaatata tcaggtgtta gatataatgg
    33721 cacagattta acgggagctt tgaaggaagc accaattaaa gatcctaaga aagcaatgat
    33781 agccacacaa caaggattta aaaagaaatt tgatcaaacg ttttttgatt cgtatggttt
    33841 tgcgaatacg tatgcattca tggtaacgga ggaaactgct aaaaaatatc atttagagac
    33901 agtttcagat ttagcaaagc atagtaaaga tttacgttta ggtatggata gttcatggat
    33961 gaatcgtaaa ggcgatggct atgaaggatt taaaaaagag tatggtttcg actttggtac
    34021 agtgagacca atgcaaatag gtctagtcta cgacgcatta aactcagaga agttagacgt
    34081 tgcattaggt tattctacag atggtcgaat tgcggcgtat gatttgaaag tacttaaaga
    34141 tgataaacaa tttttcccac cttatgctgc gagtgctgtt gcaacaaatg aattattacg
    34201 ccaacaccca gaacttaaaa cgacgattaa taagttgaca ggaaagattt cgacttcaga
    34261 gatgcaacgc ttgaattatg aagcggatgg taaaggtaaa gaacctgctg tcgtcgcaga
    34321 agaatttttg aaaaaacacc actattttga taaacagaaa ggtggtcata agtaatggaa
    34381 ggtaatttat tacagcaatt attcaattat tatgttacga actttggtta tctatgggat
    34441 ttatttttca aacacttatt aatgtctgtc tatggtgtgc tgtttgcagc tttaattggt
    34501 attccattgg gaatcttgct tgcaagatac acaaaacttt ctggatttgt aattacaatt
    34561 gcaaatataa ttcaaacagt tccagtcatt gcaatgttag ctattttaat gttagtcatg
    34621 ggcttaggtt cagaaacagt agttttaaca gtgtttttat atgcgttact tccaattata
    34681 aaaaacactt atactggtat agctagtgtt gatgcgaata ttaaggatgc tggcaaaggt
    34741 atgggaatga cacgcaatca agtgctacga atgattgaat taccgttatc tgtttcggtt
    34801 attatcggtg gcattcgtat tgccttggtt gttgcgatag gtgttgttgc cgttggatca
    34861 tttataggag cacctacgct tggtgacatt gtgattcgtg gtacaaatgc gacggatggc
    34921 acaacgttta ttttagcagg tgcgattccg attgctatca ttgcaatcgt cattgatgta
    34981 ctattaagat ttttagaaaa acgattagac ccaacaacac gacatcgtaa aaatcaatct
    35041 aatcatcggc cgcaaagtat taatatgtaa tagtagaaga tgtttattat ttagcgattt
    35101 cgtttcatga tttataaaaa atgaggctac tcaaggagct caaatagtct ttgagtagcc
    35161 gttttatagg ttgtgtttgt atgcgtttac actaaaatag caattattat catgaaagtt
    35221 tttggataaa aagcgttgat tattgtaaaa atactaaaga atgagatgtt ttatttataa
    35281 ttttctgcaa atttatgata ttgtttctta atatatcata ttaaaaattt gtttttctta
    35341 aacataggag gcttatctaa ttcatggaca catcaaaaca atttagaggt gacaaccgat
    35401 tgcttttggg tatcgtttta ggggttatta ccttttggtt attcgcgcag tcacttgtta
    35461 atcttgttgt cccattacaa tcaacatata gtagtgacgt tggaacgata aatatcgctg
    35521 ttagcttatc tgccttattt gctggtttgt ttatcgtagg tgctggtgat gttgctgata
    35581 aatttggtcg cgtcaaaatt acttatgtag gattgatatt aaatgttata ggttcgttac
    35641 tcatcattat tacacctttg ccagcatttt taattatagg tagaataatt caaggtttgt
    35701 ctgcagcatg tattatgcca tccacacttg ctattattaa cgaatattat attggtacaa
    35761 gacgacaacg tgccttaagc tattggtcta ttggttcttg gggtggtagt ggtatttgta
    35821 cgttgtttgg tggcttaatg gctacatata taggttggcg ttcaatattt gttgtttcaa
    35881 ttctattaac tttattagca atgtacttaa tcaaacatgc acctgagact aaagcagaac
    35941 caatcaaagg tatgaaagca gaagctaaaa agtttgacgt tatcggttta gtcattttag
    36001 tagtgacaat gttaagttta aatgtaatca tcacacagac gtctcatttt ggtttagttt
    36061 caccgttaat tctaggttta attatagtgt ttatctgttc attagttggt tttgtatatt
    36121 acgaaaataa aataaagcat ccacttgttg atttttcaat ttttaaaaat agaggataca
    36181 gtggtgcaac tatttcaaac ttcttattaa atggtgtagc aggtggtgca cttatcgtca
    36241 ttaacacgta ttatcaacaa caattaggat ttaattcttc gcaaacgggt tatatttcat
    36301 taacgtattt aatagcagtg ttgtcaatga ttcgtgtagg tgaaaagatt ttatctcaac
    36361 atggtccgaa gcgcccacta ttactaggaa gtggctttac agtgattggg ttaatcttat
    36421 tgtcgttaac atttttacca gaaatgtggt atatcgtatc tagtatagtt ggatatttat
    36481 tgtttggtac tggtttagga ttatatgcta caccatcaac tgatacagca gttgcaagtg
    36541 cgccagatga taagtcgggt gttgcttcag gtgtatataa aatggcgtca tcattaggaa
    36601 atgcatttgg agtagcagta tctggtacgg tttatactgt gttagcagct aatttaaatt
    36661 tgaacttagg tggtttcaca ggtatgatgt ttaatgcctt gctagcaatt gttgcatttt
    36721 tagtcatttt actattagtt cctaaaaatc aaacgaattt gtaaaaccga aatgaaagca
    36781 agttattatg tagggatttt aaaggaaatt ttgtgaaagt aagtttatca tacacactta
    36841 atgttgcgta ttgacgttta atgttaggtg tgttctttta tagacgataa aagctgtgtg
    36901 catattaagc gaatgatttt caaattgacg ctaatatgcg aaagtagtat ttttaaaatg
    36961 aacaacaacg atgaagaggg gtttatagga tgaaaattgc aattgctgga tcgggtgcat
    37021 taggaagtgg ctttggtgcc aaactatttc aagcaggata taatgtcaca cttattgacg
    37081 gatatacatc tcatgttgaa gcggttaagc aacatggatt aaatataacg attaatggag
    37141 aggcattcga gttaaacatt ccgatgtatc attttaatga tcaaccggac gagagcattt
    37201 acgatgttgt ctttctattt ccaaagtcta tgcaattaaa agaagtgatg gaagctatga
    37261 aatcacatat tgataatgaa acgattgtcg tatgtacgat gaatggtttg aagcatgaag
    37321 aagtcattgc gcagtacgtt gctcaatcac aaattgtcag aggtgttacg acttggacgg
    37381 caggtcttga aagccctgga cacagtcatt tacttggtag tggaccagtt gaaataggtg
    37441 aactagttga tgaaggtaaa gaaaatgtta taaaagttgc tgagttactt aacgaagcgg
    37501 aattgaatgg tgtcattagt aaagatttat accaatcgat ttggaaaaag atttgtgtta
    37561 atggtacggc aaatgcatta agcacagtgt tggagtgtaa tatggcatca ctgaatgaaa
    37621 gtagttatgc gaagtgtttg atttataaat taacgcaaga aatagtgcat gtagcgacga
    37681 ttgataatgt tcatttaaat gttgatgaag tctttgaata tttagttgat ttaaatgaaa
    37741 aagttggtgc gcattatcca tccatgtatc aagatttaat tgttaataat agaaaaactg
    37801 aaattgatta tattaatggc gcagttgcaa cattaggtaa aaaacgtcat attgaagcgc
    37861 cagtcaatcg ctttattact gatttaattc atactaaaga aagtcagcga cacgcacagg
    37921 attaattagt gtttttagct atgattgcaa ggaaatgaca atcacgtgat aattacggtc
    37981 attactaaga ttgaaatgta ataaataaag aacagcagta aggtactttc aaattgaaat
    38041 gatcgtggtg ctgtttttct tgattgatct tcgtcataat tcagatttgt cataggtcta
    38101 cgacatacta ttagtattta ctagacagtt tttacgacga cactttgaaa aattttgagg
    38161 caaatcattt ggaagtctca cgtgaatttt gtaaactcat caagcaagta attatattaa
    38221 aaagacaaat agagaaaagg tgtttataat gagtaaaatt tttgtaactg gtgcaacggg
    38281 ccttattggc attaaattag ttcaaagact aaaagaagag gggcatgagg ttgctggttt
    38341 tactacatct gagaatggtc aacaaaagct agctgctgtt aatgtaaaag catatattgg
    38401 tgatatatta aaagctgata ccattgatca agcgttagca gattttaaac cagaaatcat
    38461 tatcaaccaa attacggatt taaaaaatgt tgatatggca gcaaatacga aagtacgtat
    38521 tgaaggttct aaaaacttaa ttgattcggc gaaaaagcat gacgttaaga aagtgattgc
    38581 ccaaagtatt gcctttatgt atgaacctgg cgaaggatta gcaaatgagg aaacttcact
    38641 tgattttaac tcaactggtg atagaaaagt aacggttgat ggtgtggttg gtctagaaga
    38701 agaaacggct cgtatggatg aatacgttgt cttacgtttt ggctggttat atggcccagg
    38761 tacttggtac ggaaaagatg gcatgattta taatcaattt atggatggtg aagtgacact
    38821 ttcagatggc gtaacatcat ttgtgcatct tgatgatgca gttgaaacat ctattcaagc
    38881 tattcatttt gaaaatggta tctataatgt agcagatgat gcacctgtta aaggttctga
    38941 atttgcagaa tggtataaag aacaacttgg tgttgaacca aatattgata tacaacctgc
    39001 gcaaccattt gaacgtggcg taagcaatga gaagtttaaa gcgcaaggtg gtactctgat
    39061 ttataaaact tggaaagatg gcatgaatcc aattaaataa taatttatcc gtttaatata
    39121 caaagaataa agacttggtc gaatcgtgga tgatatatta tcaaacgcac ggctcgaaca
    39181 agtcttttta ttatgtcttc gttatctttg tatgaaggaa taacagaatt acaattaatg
    39241 tactgaataa tgcaattaat attgtgatta gtgctaattt aatttctatt ggcagccaag
    39301 tcagtacaaa agaccagtta ttgctaccga gaatgagata tggtaatgca tataatatga
    39361 gcgctatagc gatacataaa cataaagtta gccaacttga aaatgtggta agttttgatt
    39421 tgcgcaggat gattttcccg tgacgcattt gccaagcacg ataagctgag aatataaatg
    39481 ctaataaaat aagtgttgtc atcaaaacgg taacaatatt aaattgattt ttatattgat
    39541 tgagcataga cgcaactgtc gaatatcgct tgtgatttac aatttgtgtg ttaagatgct
    39601 caactaattt gggtacatat tccgaattta gatttgcaag cacaacaatt ccataatttt
    39661 gttttggatt tagtaaaata aatgatgaaa agttatcgag cgttcctgaa tgaaacacta
    39721 aatgttcatc attattggta aaccagccgg aagcatatgc attggcatta ggttcaccaa
    39781 ttgttgatga taaattttta tgtgattgtt gaactaatga tttgtattta tcaggtggat
    39841 taagttggaa ttttatccaa tgttccaaat cttcagttga tgtcatcata tatgctgatg
    39901 gtgtatccca aaggttaaat tcaggtttag agacgacagg tgtcgaacct tgtaattcgt
    39961 agccaatagc atcatgtttt gttttgttat tggtttgttt gaatgatgta tgtgtcatat
    40021 tcaaaggctt gagccatgaa tttgtaatat attttgtata ggattgcttc gtaacgtttt
    40081 ggataattaa acctaataaa tcatagttca tatttgagta ttcaaattct tctccgggct
    40141 tatgatgtaa ttcatcaccc ataattgcac gggttacatc atttaaacga ttatttttat
    40201 ttgttacgga gtcttcgctt gtaatatcac taggtatacc acttgtttga gccaaaagtt
    40261 gcttaatcgt aatagtttca ttttgaccat tatagttcat tttaaaatga ggcacatgtt
    40321 tagatacggc atcatttaag tttaatcgac cttcttgagc taattttaaa attgcaagac
    40381 ctgtgaaagc tttcgtatta gaagcaattt catactttgt tgtgggagaa gctttgactt
    40441 ttttatcaac atcttgataa ccataacctt tatttaaaaa aactttgcca ttttttacta
    40501 ttaaaattga tgctccagga atgtgtccct tttgtagatc atgctcgata attgtatcta
    40561 tttgttgttg cgaatcattg gttaaccgtg tcttcgtatt gctatttaat gttatatata
    40621 tagcaattga aatggcgact aaaatgataa tagaaatgga tagaaaatat agttttttag
    40681 tagtcataaa cgttcctttt tatatgttaa atttacttaa gtgcctaatg agatttattt
    40741 tattatacca gttctattga ataaattaat taaagaggta tatttaccat tatttttgac
    40801 accttatatg ttaataggtg tataattaca taaaaaaata ttaattaact ctctatactt
    40861 ttcggaaaga taggcgtata gtgatatcca tcaaaaagta taaaaaggaa taggagatta
    40921 tgttcaatca atttaaaaga cttattatag ggcaacctaa aaaaaacaga gaactaaaag
    40981 acgaaaagat atcgaagttt aaagggttag cgattctttc gtctgatgca ttatcttcag
    41041 tggcttacgg tcccgaacaa atactgatta cattatcagt agtgggtgca gttgcaactt
    41101 ggtatacttt accgattgca ggtgctgttc tgatcttatt agctgcttta attatgtctt
    41161 ataggcaaat tatttatgca tatcctaaag gtggcggcgc ttatatggtg tccaagacca
    41221 atttaggaga aaaatgggga ttgcttgcag gaggctcatt attagtcgat tacatactga
    41281 ctgtcgctgt tagtatatca tctggtgctg atgcatttgt tgctgcattc ccaagtttat
    41341 atgggcataa agtactgatt gcatgtttac ttgtactttt tattttaata ttaaatttac
    41401 gtggtttaac tgaatctgcc accgtattat cctatccagt atatcttttc attatagggt
    41461 tagtgatatt aatatttata ggtactttcc gtgtggcgac aggagatatt caaccacata
    41521 tgcatgcatc agtaggaact gcggttcctg gagtaacatt attcttgcta ttaaaagcat
    41581 tttcatcagg ggcttcatca ttaacaggtg ttgaagctat ttcaaatgcg gtaaccaatt
    41641 ttagagaacc aagtgctaat aatgctgtaa aaacgttaat tgctatgggt tcaattttag
    41701 cgtttttatt agttggtatc gttggtttag catatgttta tgggattatg ccacaaacgg
    41761 aaacgacagt tttatcacaa ttagctatgc aaatatttgg tgataatgct gccttctatt
    41821 ttgtacaggc aacgactgtg atgattttgg tattagcagc caacactgga tttacagctt
    41881 ttccgatgtt agcggcttca atgtctaaag ataaatatat gcctagaatg tttactgtta
    41941 gaggtgatcg cttaggctac tctaactcaa ttattatttt aggtgtatta gccatcattt
    42001 taattattgt atttgatggt atgactgaag atttaattcc attgtatgct gttggtgtat
    42061 ttattccatt tacactagca caattcggta tggttattaa atggattcat gaacgtccta
    42121 agaattggtt gagtaagttg tctgtgaact tacttggtgg tatcgtgaca tttattgttt
    42181 ttatgatttt actcataact aaatttagcc aagtatggcc gatacttatt ttcttgccgt
    42241 tcgtggtgat tttcttcttg aaaattaaca aacattatcg agatatcgca gaacaattac
    42301 gttctgacat tgatgttctt aatgtggacg tggtagatcg taatttagca attgtgccga
    42361 taacaagtat tacaacagca gttgataagt ctatttatta cgcacaaatg cttgcgaata
    42421 atgatgtcat tgcggtacat gtatcatttg gagatgaaga tgaaaaagca ttccaagaga
    42481 aatggaaacg tcatttccca gatgtaagat tagttatttt acactcggaa tatcgcagta
    42541 ttatacgacc aatttcacgt tttatcgata agattaatcg taaagccaac gatcaaaact
    42601 atatgattac agtagtgata ccagaattta ttactaagaa acgttggcat aatttattac
    42661 acaatcaaac tagtttgcgt atgaaattgt atttaattta tcaaaagaat gttaacgtat
    42721 gtacaattcc atttaaactt aaaaaataga ttctataatc aaatcggatt gatgaaaggt
    42781 tatttacttt tcgtcaatcc gattttttga tgttgaatat aaacaccccg tacatatgag
    42841 atgataatga ctttgtctca tatgtacggg ggtgttgtta tgtcttgctt aatgtttgga
    42901 ctgtaaagac aagacaattt cgttgtaatt gatattttct cgaatttgag ctgcaacgat
    42961 attgttgatt ttgtttttgc gaattaattc atctaaaaga tgacgttgta agtacaatgc
    43021 ttccaatttg tggttatgga acatagcttg cggtatgatt tgattgctgt tttcttgacg
    43081 ttcttttcga cgttgtgcac gccactttaa tgagcgactc atatcatagt aatgattaat
    43141 gactaaacta acctcaagta catttgtact attttgttct tcttttaagc gtaaaataat
    43201 attgtgattc acaatacgca taattttttg aacatctttg aatgaatttt caaattcttg
    43261 cttcttagat tctttgttta aggctttgta gtgcttcatc gctttaagtc taagccataa
    43321 catcttgaag aagtttgcaa atttttcaac aaatgaagca gtacggtgtg tctctgttaa
    43381 ttcgacaata ttgcggtaat tatttatatc ttgatatgtt gccttacctt tatcaattaa
    43441 acgctcaagt gtagatgttt cgattacttt tgcaatatct tctaaacgtt tgagttcttt
    43501 tgtattttga ttatcaggtt ctgaatttaa taaaaataac aattctccat agtattggtt
    43561 taatactggg cgataatttg tatcgttttt gtctttttta ctttcgtttt taaaatgttc
    43621 gataactttt tggacaatga aaattttggc agattgataa gtcatacctt taaaagtagt
    43681 atcttcggca gatggtgtaa ttaaaggtaa aacaatttgc gctaaaatta aactaattaa
    43741 taccatgaat gatgcaataa acaatagatc attacggtat tcaaaagctt gcccttgtgt
    43801 aataataaac ggcaatgtta aggccataga taacgaaatg gtcccgtgta tgccacacat
    43861 cgtcatgata aaagcgtaac gtacacgaga aggtggtgtt tcatgtgaat catgttcctc
    43921 atctaggtat gattggatat ttttcgggaa ataaaaatct ttataccaaa aataaaccca
    43981 gataaaacgg caggcgtaaa ttgctatagc gattaatata gtaatcacta ttaaaaatga
    44041 aaagttgtcc ggctctgttt ggaaaatatc tatgactaca gtaggaatca taaaacctaa
    44101 tacaacaaat acaaaaccat ttaaggcata actaaatgtg ttccaaattt gatggtaatt
    44161 catttgtagt tccgtttgtg ctctaattaa acggtcgcgt tctaaaccat ggattagacc
    44221 tgcgattaca actgcaatga tacctgaagc atgaacttct tctgctaaaa agtatacgac
    44281 aaaaggtgtt aataattgaa taaaagttaa ggtattgtta tcttttaaac ctttattagc
    44341 ggttaagtct atacgtattc taacgataac gaatccaata attgcaccaa taagtacacc
    44401 tagtattgtt gaaatgataa attgttcaac agcttggaat aatgaaaagg taccagttac
    44461 taatgcagta acagcaattt taaatgaaat gatacctgct gcatcattga gtaaagattc
    44521 accttctaaa atcgtcatag aacctttagg taataatttt ccgcgtgtaa tagcagatac
    44581 tgctactgca tcagtaggac ataaaattgc tgctattgca aaagcggctg gcattggtaa
    44641 ggcaggccaa atccaatgta taaaatagcc aacaccgact acagttgcaa atactagtgc
    44701 cattgacatt aatagtatag gtttacgata ttctagtaat tttgttcgag agacgtgggt
    44761 acctcccaca aaaagtagtg gcgcgataac ggcaaacata aatacttcag aattgaattg
    44821 gaaatcaact tgtataggaa taatgaaaat aacgacacct aatgcaattt gaataaaggc
    44881 agtaggaatt tgtgggaatc gattattgat aaccgaacta ataatcacag caaaaataaa
    44941 aattaaaaat gcttctaata gtgccataca atactcctca aaattttaat agttaatatt
    45001 ttatcacttt taaggcataa tgacatagat atattgataa aatgaagtta ttttcaaaaa
    45061 aactctagta tcggttgaac tgatactaga gcgagatgtt taaattattg attgtcatat
    45121 ctgaaatgac cgctgtcatt ttgtcgttgt tcatacgcga gcttttcagc attcgttttg
    45181 tattttttat aaaagaaaaa taaaaatata aaccagaatg gcgaaatata aatagctgct
    45241 cttgtttcgt cactaaagaa taataaaatg aatacaaaga agaagaacgc tagaataatg
    45301 taagcaatag gcttaccacc aatcaactta aatttactgt ttttatgtgc ctcaggatgc
    45361 tttttcaaat acattagata agcgacaata atcattgccc aaacaactaa aaacaacaca
    45421 gttgataacg ttgtaacgta tatgaatagt tgaattgcat tagggaaaat gtagtttaac
    45481 aaagctgcta taagtaatgc aattgatgaa actaacattg ataaataagg cacgccatga
    45541 ctattcgttt tatttaaaac tttaggacct aacccttgtt gtgacagtcc gaataagata
    45601 cgactattcg aatagatacc actatttgta gcagaggccg cggcagttag cacgacaaag
    45661 ttaacgacac ctgctgcaaa tggcacgccg attaatgtaa agagtttaac gaaagggcta
    45721 ctatttggat cgatatcatt ccaaggtatg actgacataa ttactaatag accaccgata
    45781 tagaataata aaatacggat aggtacatta ttaattgctt tcggtaaggt tttttcagga
    45841 tctttcgttt caccagcagt tacacctata agttcaatac caatgaatga atatacagca
    45901 atttggaatg acattaagaa accaaatgtt ccaccaggga acatgccacc gtgactgatt
    45961 aagtttgtga atgatgcatg tccataatgt gttttaaatg agaaaaagat taatacaaga
    46021 ccaacgataa ccatcgcaat aattgtgaca actttaataa tagagaacca aaactccagt
    46081 tcaccaaata atctggcacc taataagttg aagctaatca agattaaaac aataaatagc
    46141 acggtaatcc aatttgggac ttgtggatac caaaatgcaa agtattgtcc catcgcagtt
    46201 aggtctgaca tacttgatac aatccaacat aaccagtaag tccagccaat gacaaagcca
    46261 ccaaaagggc ctaaatattc atttgcaata tcaacaaatg aattaaatct tgtattgctc
    46321 aacaacaatt cgcctaatgc gcgcataaaa gcgaatagta caaccccaat aatcatgtat
    46381 gtgaataaca gtgatggacc agttaaagat attgtttgac ctgatcctag gaataaccca
    46441 gttccaattg cgcccccaat tgctatcaat tggatatgtc tgttattcaa ctctctattc
    46501 aattttcttg ccataatttt cctcctcagg ataaagcaaa taaaaaagtt gtgtgtaagc
    46561 gttttcttaa ttataaatac taaagaaatc catttaataa agcaattatt tttttaaata
    46621 taagaaaaaa catttgatag gtaagggtat gataactaaa acttatcgat tttttgaaaa
    46681 gatataagtt tttatgcaaa aatataaaat aatcatctaa atgaagcagt taactatata
    46741 tattagataa gctatggcat aataaataac attttaattt gagtaaagaa ggtatgggta
    46801 tgaagatagg gacaatcgcc gatttacata ttgatcgtca taataaaaaa acatccgaag
    46861 attacttaga ggcattggtt gaaatagtta agtataagaa attggatata ttgttgattg
    46921 cgggggatat ctcaaatcat tatcaattga cgcaccaatt tataacgcaa ttgacaaaac
    46981 aattagatat acctgtgaaa tttgtacctg gaaaccatga tttatgggaa gttgaaagta
    47041 tgactacgca agacatttgg aataattata agaggatgtc acagtgcttg gtaggaaaac
    47101 catttatagt aaatgaagaa tgggcaatca taggacatac tggttggtat gattatagct
    47161 ttgcagcaca acgattttca ttagatgagt tacaaaaagt aaaacattat ggtgcgactt
    47221 ggcaagataa agaacgaata tcttggggca tatcagatca aaatttatct aaaatagcgg
    47281 ttgaacaagt gaagaaagat atattagaag taggaaatag acgagtgatt ttagtcacac
    47341 atgttgtgac gcaccctgat ttcattgttc ctatgccaca tcgtatattc gatttttata
    47401 atgcatttat tgggacaagt gatttcaatc ctttgtatgc gatgttcgat ataccatata
    47461 gtattatggg ccatgttcat tttcgtaaaa gtgtgatgga tgatggcaga tgttatctct
    47521 gtccgtgtct aggctatcca agacaatggc gttcagaaga tatttaccag gaaataaatg
    47581 agacgataca aataatagaa atttaaaatg cgcaaacctg acccagtttg cgcattttat
    47641 gttttacaca cgcgagtaat gtgtttactt acgtgtgttt attttgttgt tgattttcaa
    47701 ttgtatatga atgtggttgc acataaatgc actttcttcc tggtgaatta aagctatatt
    47761 ccattttctc tttacgaatt ttaataattt gtttgcgatt tggaatgatg gcaggtaaaa
    47821 ctaggccacg acgaatatga ctccaaagtg gatcgatata atagtaataa taatcatcgt
    47881 acccaatgag taacgttacg tgaatatttg aaacttgttt cgctggcttg ttatcaaagc
    47941 ggaaaacacg tagtaatggt ttagaaccaa gattagtatg gtatattaac acaggttgac
    48001 cttgatcgat aataccttta agatcttcta aggatttacc agtgccgtct acgatattag
    48061 gattgtattt ttgtaaaaat ggtacatatg cttctggaaa tatcgtttga tgataattgc
    48121 caagcttaat gaataagtga tgtccaacat aacctttatg tggattgttc ggatgtgtcg
    48181 gccaatgtct cataatttct gtagcaggga tatgttggtt attgtattgc aacatcatgg
    48241 ctgcggaaac accttcacac cccatgacca tagggatagg aaatagctga ctgataggtt
    48301 taactggtaa tatttttcgg ttcataatat agtcctcgca ttgattcaat aaatatttaa
    48361 tataattata tagcgtcaat gcaaaatgtc ctaaacatat gttttacatg agtgaataaa
    48421 attaatggag tgataaaatg gaatatcaat tacaacaact tgcgtcgtta acgttagtag
    48481 gtattaaaga aacgtatgaa aatggacgac aggctcagca acatatagca gggttttggc
    48541 aaagatgtta tcaagaggga gtaattgcgg atttacagtt aaaaaataat gttgatttag
    48601 tcgggatact tggcttatgt atacctgaat tagacggtaa gatgtcatat atgattgcag
    48661 ttaccggaga taatagtgct gatattgaaa aatatgatgt cataacatta gcaagttcaa
    48721 agtatatggt atttgaagca cagggagcag tacctaaagc agttcaacaa aaaatggaag
    48781 aggttcatca ctacatacat caatatcaag cagatacggt aaaatcagca ccattttttg
    48841 agttgtatca ggatggtgat actacaagtg aaaaatatat tacagaaatt tggatgcctg
    48901 ttaaagggtg attgaataga aatgtacttg atatgaaggc gtaattttag ttgaagattg
    48961 gatctcataa ttcataaaga agaaccctga caaagataga tatctttgct agggttctaa
    49021 ttatgttgaa gtgttatgcg acatgttgct ttttaatcat tttaaagtaa atgatttgta
    49081 gactgactaa tagaatggct gtaatgaaga taataatcat atatggtgat gtattaaatt
    49141 cgccttttaa gccaactaat ggtgcaacag tgccacctaa gataaattgg aataagccaa
    49201 gtaaacttga tgcgttacca ctgccacctg ttcgttcttc catagccatt gtgaaaccaa
    49261 gcggtccaat tgacgtcaca ggacacacat ttaagaagaa tgcgatgagt aagacccaaa
    49321 gtggtaaatg gaatgtaagt gtcaggataa ttaaagcaac acctaccact tgtataatag
    49381 ttaaaatgat taataatatg tggcgatgta atttttctac taataaagca acgacttgac
    49441 tgacaatgat taaacctaca ccgttaacag caaacatgat actaaattgt tggggtgtca
    49501 tattatacaa tttttgagta ataaatggcg atgcagatga ataactaaat agcattacat
    49561 aagttaaacc ttgtaatagc attggaataa taaatgctgg ttttttcaaa agacttccaa
    49621 aatctttaat gacgctacta aaattcacct gctttaattc ttctttaggt gttttaggta
    49681 attgagaagc gacgccaatt aaaatgataa gtgccacaat cgttaaaatt gtgaaaatag
    49741 cacgccaagt tgctacggat aaagctaatc caccggctaa tggtgcaaga atagtgataa
    49801 taccattaac taccattaaa gatgctaaaa atttagcgag tgcgttgcca ctaaatttat
    49861 caccagcaga ggctttggca ataacgatga cgccaccacc agttaaacct tgaattaaac
    49921 gtaaagttaa aaatagaggt aattgggtaa cgaacacaga taatccggat gccaagatga
    49981 aagtgattaa gatcgataca gcaatacgtt tacgaccaaa ggcatcggat aaaggtccga
    50041 agataaattg accaagtgca agaccaatca ttgtgaatga taaggttaat tgaatttctg
    50101 atgttgtcga accaaaatca ccttgcactt taggtaatga tggtacgtac atatctatag
    50161 ttagagggcc aaaagctgtc atgacaccta acgtgattaa caataaaaat gaaaattgct
    50221 tcgttttgga actcgaagat tgcataaaca gactccttaa tttatataat tatgtaaatt
    50281 atatacgttt tatgtgcatt tgtatgtagt tagaatataa gtttatataa aaagtcctat
    50341 actcgcaatg tatcatcatt aataacgtag tgatacagat aaagtatagg acttaatgtt
    50401 tatcatcaaa gcaggtgtaa taattaatta gcgccaccat tgtcatcttt aaataagctt
    50461 tctaatttag atttaatact gtcgaatttt gtattaatat catttaacat gctttcaatt
    50521 ttaccttggc ttgcattgtt tttaagtgca ttatttgcct ctgtcaattt gttgatttgg
    50581 tcttgataaa attgagttag cttagaatca gctttgtttt gttcttgctg cttcaagttg
    50641 tcgatttgat tttgtaaatt tttaataatg tcatcagttt tttggttgtt tttaatattt
    50701 tctttagcat tgtcgacttg ctggttaatt tggccgtctt gattttcaac ttgtttttta
    50761 taattttgag agactttagg gtcattacta actttatcgc tatcattgtt aagtaaggcg
    50821 cgtgtgacaa aaatagcgac aataattaag agaatagcaa taatgatata taaccatttt
    50881 tttgaacggc gggatttctc gttttcttct tcaagaattt gttctcttcg ataatctcta
    50941 ccattatagt atcgttccgg gtgttcttca ggatcgcctt tagcatattg ttgtctatac
    51001 gatgcgtctt cagattgacg acgatgattt tgattttgat tgtcgtattg atatgaatca
    51061 cggtatttat cagttctttt catgggtttc acctcattca agttttaact gcaaataata
    51121 aagctgatga tttactttga cattattgta atttcagaat acgagcaatg tcagttgcag
    51181 tgtttaataa attttgttcg ctattttgta ggacatcttc taaagtgcta ggtgaagaga
    51241 ttatagaata ggcactatca ataccgaaat cgtaaacatg ttgataattt tcgcctagac
    51301 ttccacaaat cgcgatgaca ggaatatgat attgttttgc agctaacgca acgcctacgg
    51361 gtgttttacc aaagatggtc tgataatcca tgcgtccttc cccagtaata acgaggtctg
    51421 catctttaat tctttgatga aaatctgtaa tgtcaaagac gacatcaata ccttttgtta
    51481 aagttgtctc acaaaacgct aataatgctg cgcccatacc gccagctgca ccagaacctg
    51541 gtatttgatt aacggacttt cctgtgcaca tttttatctt atcatgataa tgcgacattg
    51601 cgaaatccaa ctttggtatc atctttgcat cagcgccttt ttgaggacca taaatatagg
    51661 tagcaccatt ttcacccaat aaaggatttg aaacatcaca ggccacttta aaggtcacct
    51721 cttttaatcg cgaatctaga ttggttatat cgatttgtgc aatgtgagca agattagcac
    51781 cattcatttg taataagtcc ccgtttacat cagtaaactt tacgcctagt gcacttagca
    51841 tacctgtacc accatcattt gttgcactgc caccaatccc taaaataatg gtcttagcac
    51901 catgatttaa tgcatcttta attagttcac cggtaccata tgatgatgtg tataaaggat
    51961 tacgttcctc tttttctaat aaatccaaac ctgacgctgc cgccatttca ataattgcaa
    52021 tttgttgttc gtctgcgcgt gcataacatg cttcaatagg tcgcattaaa gggtcattaa
    52081 cgatgactgt atacttagtg gcacctgttg catgcattaa agcatctgtg gtaccttcac
    52141 caccatcagc catcggaatg atatcataat gaagggtatt cccataaaca ttagtaaatg
    52201 cctgctttat aatattgcca acttgctgtg cggtcatact ttccttaaat gagtcaggag
    52261 cgatgacaat tttttgtaat tgcatagtag acatcctctc attactgttg tcgctcttat
    52321 tgtatataaa attactagtg aactaaagta atctctatag tgctaatgag tcgcgcgata
    52381 aattagttac attataatgg cgtataagga tgaggggaaa gagactaaat ttgctgtgaa
    52441 agggtataaa aattgaagta agtatatttg aagagaagcg gattggctcg catattcgag
    52501 tatgttggag aacgtagtta tacatatatt ttgatgttga ggagttacac atatgaaact
    52561 aacacaaacg catgctgaaa ttttaaaatt tatcattgtt ggcggcatta atacgttaaa
    52621 ttattatgtt gtctatttat tgctgttaaa attattacac attgaatata tgattagtca
    52681 tattaccgga tttatagtcg cttttgtgat ttcatattat ttgaattgtt attttgtcta
    52741 cagagtaaaa cctacttgga gaaaattcat tagttttcca attacgcagc ttgtcaacgt
    52801 aagcttacaa acagttttat tatatgtttt tgtatcctgg ctaaatttgc cagcggaaat
    52861 tgcgccattt gcaggtttag tgattacgat acccatcaca tttatattat ctaaatggat
    52921 tttaaaagat agtaattaaa gtagcgtaaa ccactaaaga atactttgct caggcgttaa
    52981 tattgcgtga aatataataa ataaatgata taatataatg atcgcctaac atctcgggcg
    53041 ttactttaac gcagagagga ggtgtatgac ggatgttcaa tttattaatt gacatcatga
    53101 cttcagcttt aagcggctgt cttgttgcgt tttttgcaca ttggttacga acgcgcaaca
    53161 ataaaaaagg tgacaaataa ccaacaccta aataaatccc ctcactatgg cagtagtgag
    53221 gggatttggt gtatttagat gtt
//
LOCUS       DAHHOJ010000003        52150 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.003, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000003 DAHHOJ010000000
VERSION     DAHHOJ010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 52150)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52150)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52150
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.003"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            31..294
                     /locus_tag="NBH32_000129"
     CDS             31..294
                     /locus_tag="NBH32_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1513772.1"
                     /translation="MNMHILYNLRTKHNLEIDELAQQLNEKYGTKYEAHQIWEWENHH
                     HEPKFKDAMHLADFFDVPYEMFLESKVKEYQKHLEEVDIRMDK"
     gene            complement(433..1005)
                     /locus_tag="NBH32_000130"
     CDS             complement(433..1005)
                     /locus_tag="NBH32_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1513773.1"
                     /translation="MKLKSLAVLSMSAVVLTACGNDTPKDETKSTESNTNQDTNTTKD
                     VIALKDVKTSPEDAVKKAEETYKGQKLKGISFENSNGEWAYKVTQQKSGEESEVLVAD
                     KNKKVINKKTEKEDTVNENDNFKYSDAIDYKKAIKEGQKEFDGDIKEWSLEKDDGKLV
                     YNIDLKKGNKKQEVTVDAKNGKVLKSEQDH"
     gene            complement(1186..1554)
                     /locus_tag="NBH32_000131"
     CDS             complement(1186..1554)
                     /locus_tag="NBH32_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1513774.1"
                     /translation="MKTILKTITYLALTIVGAYAALFILKTIDSHGITDQFNPLVKED
                     DSYVKTTEVSTRMDDQLRSYTQSAFNKEGKETQLMYTATFDVKPHRYLKITHKGHHVE
                     TFEEVEKEEVPKKALDKLSR"
     gene            complement(1923..2534)
                     /locus_tag="NBH32_000132"
     CDS             complement(1923..2534)
                     /locus_tag="NBH32_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCD1513775.1"
                     /translation="MFKKHHSKNSILLKSILSLGIIYSGSFGIYSKAGASTQNSSSLQ
                     DKQFQKVEEVPNNSEKALVKKLYDRYSKNTINGKSNKSRNWVYSERPLNENQVRIHLE
                     GTYTVADRVYTPKRNITLNKEVVTLKELDHIIRFAHISYGLYMGEHLPKGNIVINTKD
                     GGKYTLESHKELQKDRENVKINTADIKNVTFDLVKSVNDIEQV"
     gene            2841..3464
                     /locus_tag="NBH32_000133"
                     /pseudo
     CDS             2841..3464
                     /locus_tag="NBH32_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015977853.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
     gene            3503..4441
                     /locus_tag="NBH32_000134"
     CDS             3503..4441
                     /locus_tag="NBH32_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Abi family protein"
                     /protein_id="HCD1513776.1"
                     /translation="MLNFDEQIAKLKQMNIFFNIIDTEKANEILRKNNYFFKLAYFRK
                     NFEKKNGGYFIEFAYLSDLATIDMKLRYTMLHLTLDIEHSLKCLVLKLITENNQEDGY
                     KIIDEFLCIDKSYNNSNFDTNSRTPEEVMETKIKNKNEIFKHMNKRGQLPAKLNKYYQ
                     NPPAWVCIEFMQLGQFVSFLNFYYKKYNDEELRVANILMPLVKNIRNKSAHNQPIIAN
                     LNYDSRSPQYLFEKGNNIGISRNMFGIKNFIDTFATLELHNQVCSNAIIQARYHDLDQ
                     LQKRYKRNESYYNNALAIKRFFIALDKIIDFNRPKV"
     gene            complement(4707..4949)
                     /gene="rpsR"
                     /locus_tag="NBH32_000135"
     CDS             complement(4707..4949)
                     /gene="rpsR"
                     /locus_tag="NBH32_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002484306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCD1513777.1"
                     /translation="MAGGPRRGGRRRKKVCYFTANGITHIDYKDTELLKRFISERGKI
                     LPRRVTGTSAKYQRMLTTAIKRSRHMALLPYVKEEQ"
     gene            complement(5001..5504)
                     /gene="ssb"
                     /locus_tag="NBH32_000136"
     CDS             complement(5001..5504)
                     /gene="ssb"
                     /locus_tag="NBH32_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1513778.1"
                     /translation="MLNRVVLVGRLTKDPEYRTTPSGVSVATFTLAVNRTFTNAQGER
                     EADFINCVVFRRQADNVNNYLSKGSLAGVDGRLQSRNYENQEGRRVFVTEVVCDSVQF
                     LEPKNAQQNGGQRQQNEFQDYGQGFGGQQSGQNNSYNNSSNTKQSDNPFANANGPIDI
                     SDDDLPF"
     gene            complement(5525..5821)
                     /locus_tag="NBH32_000137"
     CDS             complement(5525..5821)
                     /locus_tag="NBH32_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCD1513779.1"
                     /translation="MRTYEVMYIVRPNIEEDAKKALVERFNGILATEGAEVLEAKDWG
                     KRRLAYEINDFKDGFYNIVRVKSDNNKATDEFQRLAKISDDIIRYMVIREDEDK"
     gene            6066..6257
                     /locus_tag="NBH32_000138"
     CDS             6066..6257
                     /locus_tag="NBH32_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513780.1"
                     /translation="MNLNIDWSKDFQEFQEILNSGIHPEWLYCAKANLVLEPAYTGEG
                     KQFFSTQDIINASKIIPFF"
     gene            complement(6343..7440)
                     /gene="ychF"
                     /locus_tag="NBH32_000139"
     CDS             complement(6343..7440)
                     /gene="ychF"
                     /locus_tag="NBH32_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCD1513781.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGALAANYPFATIDPNVGIV
                     EVPDARLLKLEEMVQPKKTLPTTFEFTDIAGIVKGASKGEGLGNKFLSHIREVDAICQ
                     VVRAFDDDNVTHVAGRVDPIDDIEVINMELVLADLESVEKRLPRIEKLARQKDKTAEM
                     EVRILNTIKEALENGKPARSIDFNEEDQKWVNQAQLLTSKKMLYIANVGEDEIGDDDN
                     DKVKAIREYAAQEDSEVIVISAKIEEEIATLDDEDKEMFLEDLGIEEPGLDRLIRTTY
                     ELLGLSTYFTAGVQEVRAWTFKQGMTAPQCAGIIHTDFERGFIRAEVTSYDDYVQYGG
                     ESGAKEAGRQRLEGKEYIMQDGDIVHFRFNV"
     gene            complement(7452..7655)
                     /locus_tag="NBH32_000140"
     CDS             complement(7452..7655)
                     /locus_tag="NBH32_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HCD1513782.1"
                     /translation="MASKYGINDIVEMKKQHACGTNRFKIIRMGADIRIKCENCQRSI
                     MIPRQTFDKKLKKIIVSQDDTQR"
     gene            complement(7685..8566)
                     /locus_tag="NBH32_000141"
     CDS             complement(7685..8566)
                     /locus_tag="NBH32_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HCD1513783.1"
                     /translation="MDQVMSIISSLFEPLTKIETYESVATKLAMIIIYIIVALIVIKI
                     LDKMIAQGFKIQNKSKKGNKKRSKTLISLVQNVVKYIVWFIVVTTILSKFGISVEGVI
                     ASAGVVGLAVGFGAQTIVKDVITGFFIIFENQFDVGDYVKINNGGTTVAEGTVKSIGL
                     RSTRINTISGELTILPNSSMGEITNYSITNGTAIVKIPVSVDENIDNVEKKLNKLFTS
                     LRSKYYLFVSDPVVIGIDAFEDTRVILRVSAETIPGEGFSGARIIRKEVQKMFLQEGI
                     KTPQPIMTPFNHSENGV"
     gene            complement(8720..9565)
                     /locus_tag="NBH32_000142"
     CDS             complement(8720..9565)
                     /locus_tag="NBH32_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCD1513784.1"
                     /translation="MSELSKSEDQRITKTKYEQIKQIDISDIKPNPYQPRKTFDENHL
                     NDLADSIKQYGILQPIVLRKTVQGYYIVVGERRFRASKIAGLKYVSAIIKDLTDEDMM
                     ELAVIENLQREDLNAIEEAESYQRLMTDLKITQQEVAKRLSKSRPYIANMLRLLHLPK
                     KIADMVKDGRLTSAHGRTLLAIKDEQQMLRLAKRVVKEKWSVRYLENHVNELKNVSSK
                     SETDKVDITKPKFIKQQERQLREQYGAKVDISIKKSVGKISFEFESQDDFVRIIEQLN
                     RKYGK"
     misc_binding    9794..10163
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            10227..11330
                     /locus_tag="NBH32_000143"
     CDS             10227..11330
                     /locus_tag="NBH32_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1513785.1"
                     /translation="MKDTQLAQITLTDDSTGAIANPIHLSTAYKHPKLGQSTGFDYTR
                     TKNPTRSTFETCFAKLEHGIASFATSSGMSAIQLICNLFKPHDEILVSFDLYGGTFRL
                     FEFYEQQYDIKFKYVDFTDYEQVEKEITDKTVALFIEPISNPQMIAIDVKTYYQLCKA
                     KGLLSIIDNTFLTPYLSTPLAEGADIVLHSATKYIGGHNDVLAGVVTVKDESLAQQLF
                     DFHNMTGATLSPIDSYLLLRGLKTLHLRIERAQSNARKLAKKCQSLQAIDEVLYSGQT
                     GMLSLRLNKAYSVAKLLENLDICIFAESLGGTETLVTFPYTQTHVDMPDAEKDKRGID
                     EYLIRLSLGVENYEDIEHDIIQALDKAQIGEIV"
     gene            11327..12487
                     /locus_tag="NBH32_000144"
     CDS             11327..12487
                     /locus_tag="NBH32_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent transferase"
                     /protein_id="HCD1513786.1"
                     /translation="MTLSKETEVIFDWRRGVEYHSANPPLYDSSTFHQTSLGGDVKYD
                     YARSGNPNRELLEEKLARLEQGKFAFAFASGIAAISAVLLTFKSGDHVILPDDVYGGT
                     FRLTEQILNRFNIEFTTVDTTKIEQIEGAIQSNTKLIYIETPSNPCFKITDIKAVSKI
                     AEKHELLVAVDNTFMTPLGQSPLLLGADIVIHSATKFLSGHSDLIAGAVITNNEAISE
                     ALYLIQNGTGNMLSAQDSWTLAKHLKTFPIRFKQSVENAQKIVSFLIKQDEISEVYYP
                     GLTASHLEQAKNGGAVIGLRLADESKAQQFVDALTLPLVSVSLGGVETILSHPATMSH
                     ATLPEEVRQERGITFGLFRLSVGLEDPDELIADIKYALKEAFNESIPHTIER"
     gene            12456..14297
                     /locus_tag="NBH32_000145"
     CDS             12456..14297
                     /locus_tag="NBH32_000145"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HCD1513787.1"
                     /translation="MSQFLTQLKDNVLVADGAIGTILYSEGLDTCPEAYNLSHPDKVE
                     RIHRSYIEAGADVIQTNTYGANFEKLKRFGLEDKVKAIHQAAVRIAKKAANKDTYILG
                     TVGGFRGIKQEDISLQTILYHTEIQIDTLIEEGVDALLFETYYDLEELTNVISRTRKK
                     YDIPIIAQLTASNTNYLVNGQAINEGLKQLVQCGANIVGLNCHHGPHHMQESFTHIEL
                     PEHAYLSCYPNASLLDIENSKFKYSDNAQYFGQVAQNLIREGVRLIGGCCGTTPEHIK
                     FIKESIQTLKPVNDKKVIPIPTKALFNPSQNKVRQSLTSKVQERPTVIIELDTPKHLD
                     TDRFFENIAKLDKANVDAVTLADNSLATVRISNIAAASLIKQYYNIEPLVHITCRDRN
                     LIGLQSHLLGLSLIGVNEILAITGDPSKVGHLPGATNVYDVNSKGLTELALRFNQGIN
                     TDGDALKKRTHFNIAGAFNPNVRKLDGAVKRLEKKIESGMSYFITQPVYSKEKIIEIY
                     HATKHLNKPFFIGIMPIASYKNALFLHNEVPGIKMSDEILQQFEAVKDDKAKTRELSL
                     KLSKDLIDTVHEYFNGLYIITPFQNVEDSLELAAYSKSITAHKEAIL"
     gene            14294..16522
                     /gene="metE"
                     /locus_tag="NBH32_000146"
     CDS             14294..16522
                     /gene="metE"
                     /locus_tag="NBH32_000146"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCD1513788.1"
                     /translation="MTTIKTSNLGFPRLGRKREWKKAIESYWAKKISKEELDQTLTDL
                     HKENLLLQKYYHLDSIPVGDFSLYDHILDTSLLFNIIPERFQGRTIDDDLLFDIARGN
                     KDHVASALIKWFNTNYHYIVPEWDNVEPKVSRNVLLDRFKYAQSLNVNAHPVIVGPIT
                     FVKLSKGGHQTFEEKVKTLLPLYKEVFESLIDAGAEYIQVDEPILVTDDSESYENITR
                     EAYDYFEEAGIAKKLVIQTYFEHAHLKFLSSLPVGGLGLDFVHDNGYNLKQIEAGDFD
                     KSKTLYAGIIDGRNVWASDIEAKKVLIDKLLAHTNELVIQPSSSLLHVPVSLDDETLD
                     TSVGEGLSFATEKLDELDALRRLFNQNDSVKYDKLKARYERFQNQSFKNLDYDFESVR
                     TSRQSPFAQRIEQQQKRLNLPDLPTTTIGSFPQSREVRKYRADWKNKRITDEAYETFL
                     KNEIARWIKIQEDIGLDVLVHGEFERNDMVEFFGEKLQGFLVTKFGWVQSYGSRAVKP
                     PIIYGDVKWTAPLTVDETVYAQSLTDKPVKGMLTGPVTILNWSFERVDLPRKVVQDQI
                     ALAINEEVLALEAAGIKVIQVDEPALREGLPLRSEYHEQYLKDAVLSFKLATSSVRDE
                     TQIHTHMCYSQFGQIIHAIHDLDADVISIETSRSHGDLIKDFEDINYDLGIGLGVYDI
                     HSPRIPTKEEITTAINRSLQQIDRSLFWVNPDCGLKTRKEEEVKDALTVLVNAVKAKR
                     QE"
     gene            16565..17317
                     /locus_tag="NBH32_000147"
     CDS             16565..17317
                     /locus_tag="NBH32_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HCD1513789.1"
                     /translation="MTQYPLWQQLNTLKQATWVDLTHTFDPEIPRFSEFEKGEVSTLF
                     TVKDHGFYVQRWNIVTQYGTHIDAPIHFVENKRYLEDIDLKELVLPLIVLDFSTEVAN
                     NNDFIVTRAHIEAWEKEHGTIEPGTFVALRTDWSKRWPNIETFENKDANGQQHAPGWG
                     LDALKYLIEERRVEAVGHETFDTDASVDVVKNGDLVGERYILGQDKYQVELLTNLDQL
                     PTRGAIIYAISPKPKDAPGFPVRAFAIKPSND"
     gene            complement(17400..18581)
                     /locus_tag="NBH32_000148"
     CDS             complement(17400..18581)
                     /locus_tag="NBH32_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCD1513790.1"
                     /translation="MTRVVLAAAYRTPIGVFGGAFKDVPAYDLGATLIEHIIKETGLN
                     PSEIDEVIIGNVLQAGQGQNPARIAAMKGGLPETVPAFTVNKVCGSGLKSIQLAYQSI
                     VTGENDIVLAGGMENMSQSPMLVNNSRFGFKMGHQSMVDSMVYDGLTDVFNQYHMGIT
                     AENLVEQYGISREEQDTFAVNSQQKAVRAQQNGEFDSEIVPVSIPQRKGEPIVVTKDE
                     GVRENVSVEKLSRLRPAFKKDGTVTAGNASGINDGAAMMLVMSEDKAKELNIEPLAVL
                     DGFGSHGVDPSIMGIAPVGAVEKALKRSKKELSDIDVFELNEAFAAQSLAVDRELKLP
                     PEKVNVKGGAIALGHPIGASGARVLVTLLHQLNDEVETGLTSLCIGGGQAIAAVVSKY
                     K"
     gene            19120..20253
                     /locus_tag="NBH32_000149"
     CDS             19120..20253
                     /locus_tag="NBH32_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HCD1513791.1"
                     /translation="MVNALTPKHLAEKRAGFHELFFDLIFVYAIQKIAHVILTTQNGS
                     ISADLFFKYIVMSLFLWLMWSHQTFFTNRFGQVTFKDVSFMMFNMFIMVFLSNSLYPD
                     FEKTFFPFFLCVAIMYLSIGFQYLLHIRTGLDYGDKRTCQAFAAVAFVISFLSFLSLV
                     LPQSIHYIPGFLGVFIAATGLIPFQKYLVLSPVNMMHLVERFSLLTIIIFGEVLVGLA
                     SSSFSIDHFSYIYIFQFMILISLFGVYWIITENYINHKLSSIGFRLSYTHLLINIALG
                     VINAAIVFSNNNKLNDLFEINMMYISVLIFYIGLWLITPYFHNELTNAKYISSSLGIL
                     VVSYIISLIFKGHDQVMIISVSVATFCIMLIYFKNQRLRQSDE"
     gene            complement(20643..21272)
                     /locus_tag="NBH32_000150"
     CDS             complement(20643..21272)
                     /locus_tag="NBH32_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-2 transporter permease"
                     /protein_id="HCD1513792.1"
                     /translation="MKGMFLSSFYATRMQTYIYFIVAIIAAGYFAVFNPLMSSAMAGV
                     MLITPITDNIKHEKDSRWMYYVSTLPVKRSDYIKSYFAFYLILFGASLMIGLVVTTIV
                     TQSVMIGIMSGLMSFGIIGAYSIIFPLTFKFGAENSNVIMIFASILLLISFVVFFFIY
                     GMVSGASALEFEKISTEGWLVVIVYAVIGIVITSVSYILSIKIFNKQEL"
     gene            complement(21272..22114)
                     /locus_tag="NBH32_000151"
     CDS             complement(21272..22114)
                     /locus_tag="NBH32_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1513793.1"
                     /translation="MTTLLNVDSVNKQYKDSDFKLQDASLTISTNETVGLIGKNGSGK
                     STLINILVGNRHKDNGSITFFDEEHAMDDVEYKEHIGIVFDDLRVPNKLTIKDIDKVF
                     QSIYTTWNSQKFFDLIKYFELPLQTKIKTFSRGMRMKIALTIALSHDVKLLILDEATA
                     GMDVSGREEVMELLEDFVAQGGGILISSHISEDIEQLADKLVFMKDGRMILTEQKDIL
                     LAQYGIVTTEDKDVEIPKHLIIASRLSKGKYQILVKDYAEIENAEPLKHIDDATKIIM
                     RGEV"
     gene            complement(22139..22852)
                     /locus_tag="NBH32_000152"
     CDS             complement(22139..22852)
                     /locus_tag="NBH32_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3169 family protein"
                     /protein_id="HCD1513794.1"
                     /translation="MKILRYIGYLLLGGLVGGIIGGILGNFDGLGIENLTFATYNNVV
                     VISIVATIIIILVEIIVLMNQRRALKYKRLVDEEVDIDATDQYELLANRYVLNGSILS
                     IIQTIIAFVVLLIFVVGHAEANAILFFLIPFFASAIFNTQFTLFNRRFDDRMPKIADK
                     NYTEKRLEILDEGERHIELIALFKTYAINLSILILAIVVIGLYSITTGVNQSFSLLLI
                     IAIFIYNAFSYLLKRRRFY"
     gene            complement(22849..23052)
                     /locus_tag="NBH32_000153"
     CDS             complement(22849..23052)
                     /locus_tag="NBH32_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1513795.1"
                     /translation="MRNRLKELRARDGLNQTQLAKQAGVSRQTISLIERNNFMPSVLT
                     AIKIARIFNEKVETIFIIEEDEA"
     gene            23293..23682
                     /locus_tag="NBH32_000154"
     CDS             23293..23682
                     /locus_tag="NBH32_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 family protein"
                     /protein_id="HCD1513796.1"
                     /translation="MAFTLSAIQQAHQQFTGVDFPKLFKAFKDMGMTYNIVNIQDGTA
                     TYVHQSEDDIVTSSIKSNHPVAPRSNQSIVQDVLTRHQQGQTDFETFCDEMAEAGIYK
                     WHIDIQAGTCTYIDLKEQAIISELIPQ"
     gene            23782..24006
                     /locus_tag="NBH32_000155"
     CDS             23782..24006
                     /locus_tag="NBH32_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HCD1513797.1"
                     /translation="MIIMITNTFILGITGPTSLVVISIIALIIFGPKKLPQFGRAIGS
                     TLKEFKSATEDLDKESHDTPSKESKQQREQ"
     gene            24023..24724
                     /gene="tatC"
                     /locus_tag="NBH32_000156"
     CDS             24023..24724
                     /gene="tatC"
                     /locus_tag="NBH32_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006191819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HCD1513798.1"
                     /translation="MVHFSELRHRLVKILLSFVITVIVVYVSSFWWMTPFITYITRAH
                     VSLHAFSFTEMIQIYVMIIFFIAFCFISPVMFYQLWAFIAPGLHNNERQFIYKYSFFS
                     VLLFCAGVAFAFYVGFPMIIQFALKLSLTLNISPVIGFKAYLVELIRWLFSFGILFQL
                     PILFIGLAKFGLIDTTSLKHYRKYIYFACFVLASIIAPPDITLNILLTLPLILLFEFS
                     MFIVKFTCRGKPPTH"
     gene            complement(24817..26529)
                     /locus_tag="NBH32_000157"
     CDS             complement(24817..26529)
                     /locus_tag="NBH32_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000683714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family iron permease"
                     /protein_id="HCD1513799.1"
                     /translation="MKHYLTKIIAMLITAAMVCSFGLLKSQAAAQQSISDVYSVITDA
                     KSVLSNNSISNDNKQKAIEQVVSAVKKLSLEDNSEGNAVKSDVRKLEDAKANDDQKDT
                     LSQLTKSLIAYEEKLASKDAGSKIKLLQQQVDAKDAAMTKAIKDKNKAELESLNNSLN
                     QIWTSNETVIRNYDANQYGQIEVALLQLRIAIHKSPLDTTKVSHAWTTFKSNIDHVDK
                     KSGTSANDQYHVSQLNDELEKAIKAIDDNQLSDADAALTHFIEIWPYVEGQIQTKDGA
                     LYTKIEDKIPYYQSVLDEHNKAHVKDGLVDLNNQIKEVVGHSYSFVDVMIIFLREGLE
                     VLLIVMTLTTMTRNVKDKKGTASVIGGAIAGLVLSIILAITFVETLGNSGILRESMEA
                     GLGIVAVILMFIVGVWMHKRSNAKRWNDMIKNMYANAISNGNLVLLATIGLISVLREG
                     VEVIIFYMGMIGELATKDFVIGIVLAIVILIVFALLFRFIVRLIPIFYIFRVLSIFIF
                     IMGFKMLGVSIQKLQLLGAMPRHVIEGFPTINWLGFYPSYEPLIAQAAYIMVVAILIF
                     KFKK"
     gene            complement(26510..27738)
                     /gene="efeB"
                     /locus_tag="NBH32_000158"
                     /pseudo
     CDS             complement(26510..27738)
                     /gene="efeB"
                     /locus_tag="NBH32_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
     gene            complement(27735..28589)
                     /locus_tag="NBH32_000159"
     CDS             complement(27735..28589)
                     /locus_tag="NBH32_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513800.1"
                     /translation="MKKLTTLLLASTLLIAACGNDDSKKDDSKTSKKDDGVKAELKQA
                     TKAYDKYTDEQLNEFLKGTEKFVKAIENNDMAQAKALYPKVRMYYERSEPVAEAFGDL
                     DPKIDARLADMKEEKKEKEWSGYHKIEKALYEDKKIDDVTKKDAQQLLKDAKELHAKA
                     DTLDITPKLMLQGSVDLLNEVATSKITGEEEIYSHTDLYDFKANVEGAQKIYDLFKPI
                     LEKKDKKLSDDIQMNFDKVNQLLDKYKDNNGGYESFEKVSKKDRKAFADAVNALGEPL
                     SKMAVITE"
     gene            complement(28854..29399)
                     /locus_tag="NBH32_000160"
     CDS             complement(28854..29399)
                     /locus_tag="NBH32_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1513801.1"
                     /translation="MFGMKVNEQIRLKILEAHDTEALFNLINRSRNSLREWLPWVDAT
                     EQPSDTRAFIKRGLLQFADGHGFQCGIWYEGMLVGVIGLHEINHMHRKTSIGYYLDKQ
                     FEGHGIMTQAVEALIKYCFDEIDLNRIEISAAVHNEKSRAIPERLGFTREGMLRDNEL
                     LNGIYSSSYIYSLLKSEYNEG"
     gene            29760..30755
                     /locus_tag="NBH32_000161"
     CDS             29760..30755
                     /locus_tag="NBH32_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCD1513802.1"
                     /translation="MASLKNKHFMIGLSLTFIVALFSFLAAKLPILDKVGALTIAILI
                     AILYRHFRGYPEQYSSGITFSSKYLLRFAIILYGLKLNIFDIIGQGSRLLAIDVGVVI
                     FSIVMMLFVNKLLHGDKNIALLLGVGTGVCGAAAIAAVAPIFKSREKDTAISIGIIAL
                     IGTIFSLIYTAIYAIFSMTTNVYGAWSGVSLHEIAHVVLAGGFGGSDALKIALLGKLG
                     RVFLLIPLTIVLILIMRFRSSESSSKGRISIPYFLIGFVIMALVNTYVTIPSALLNIL
                     NTVSTICLLMAMVALGLNVAFKDLKNRALKPLMTIIITSICLSSLAFIVVHWLYS"
     gene            complement(30814..31380)
                     /locus_tag="NBH32_000162"
     CDS             complement(30814..31380)
                     /locus_tag="NBH32_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1513803.1"
                     /translation="MNIVLLSGSTVGSKTRIAMDDLKNELEVINEGHQIELMDLRDLE
                     LEFSVGKNYLDTTGDVYKLTTSLMQADVIFIGFPIFQASIPGALKNVFDLLPVNAFRD
                     KVIGLVATAGSSKHYLIPEMHLKPILSYMKAHTMQTYVFIEEKDFSNQQIVNDDVVFR
                     LKALAQSTMRTAKVQQHVLEEENNQYDF"
     gene            complement(31394..32455)
                     /locus_tag="NBH32_000163"
     CDS             complement(31394..32455)
                     /locus_tag="NBH32_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1513804.1"
                     /translation="MAKLEMNKNTPLEFGLYSLGDHLLNPLKGEKVSYEQRINEIIEA
                     SKLADEAGIDVFAVGESHQEHFTTQAHTVVLGAIAQATKHIKVSSSSTIISATDPVRV
                     FEDFATLDLISHGRAEIVAGRASRTGIFDLFGYDLKDYDELFEEKLGLLLELNKTERI
                     TWSGKYRPELRNMKIFPRPIDNTLPIWRAVGGPPASAIKAGKQGVPMMITTLGGPAMN
                     FKGSIDAYRQAATEAGFDASPKSLPVSTASLFYTAETTQDAMREFYPHLNTGMSFIRG
                     VGYPKQQFANSSDYREALMVGSPQQIIEKILYQHELYGHQRFMAQLDFGGVPFENVMK
                     NIELIGNDIIPAIKKHLSK"
     gene            complement(32469..33395)
                     /locus_tag="NBH32_000164"
     CDS             complement(32469..33395)
                     /locus_tag="NBH32_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1513805.1"
                     /translation="MNIVGHHHISMYTKDAKRNKDFYTNVLGLRLVEKSVNQDNPSMY
                     HLFYGDEVGTAGTILSFFEIPNAGHKQPGTETIYRFSLLVPNQAALHYFEKRLDNNGI
                     KSERLYYLGQEGIVFKDEDDLEIILLVNDSFEVPHQWQHNAYSEIPQAYQILGIGPVE
                     LRVRNAARTVEFLENVLGYRKRDNKSFDVLTLAPQGLYSDFVVIEQQGQRERPGRGYI
                     HHIAVNTPQMSDLDAIYKKLQQQPQSNSGIIDRYFFKSLYYRHNSIMYEFATEAPGFT
                     IDTPLEQLGSQLNLPDFLEAEREQIESKLHEI"
     gene            33707..35065
                     /gene="glpT"
                     /locus_tag="NBH32_000165"
     CDS             33707..35065
                     /gene="glpT"
                     /locus_tag="NBH32_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCD1513806.1"
                     /translation="MNFLKPAKHIKPLPENQIDDTYKRLRLQVFLGIFIGYAGYYLLR
                     KNFSLAMPALQEQGFTKAELGFALSAVSIAYGFSKFFMGTVSDRSNARIFLVLGLALT
                     AIVNLLMGFVPFFTSGIGIMFVLLFLNGWFQGMGWPPSGRVLVHWFSVSERGSKTALW
                     NVAHNVGGGIMAPIAAWGITTTAFINFGYLKGFEGVFIYPALLALIIAAISYVLIRDT
                     PQSQGLPPIEIYKNDFATSDKKTLETELTTKEILFKYVLNNKWVWAIAFANIFVYFVR
                     YGVLDWAPVYLSEEKHFDLKASGWAYFLYEWAGIPGTLLCGYISDKLFKGRRGPAGFF
                     FMLGVTVFVLIYWLNPPGNAWLDNVSLIAIGFLIYGPVMLIGLQALDYVPKKAAGTAA
                     GLTGLFGYLFGAVMANIVLGAVVDKFGWDVGFILLTAISVFAMLSFILTWNKVGQETV
                     HH"
     gene            complement(35025..35501)
                     /locus_tag="NBH32_000166"
     CDS             complement(35025..35501)
                     /locus_tag="NBH32_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MepB family protein"
                     /protein_id="HCD1513807.1"
                     /translation="MYKSKILLKYIFSEELDVKDLTEEKYNQDYEALTFSFKEETYQS
                     RLAKKTPTKAGYFVACWTKDENNCNQPYSKEAFADYLMIIVIDEELSGYFLFPRELLV
                     EKGILTTFEHKGKMAFRVYPKWCNQLNKTAGQTQKWQCKYFLSFNDERFLVLLYSK"
     gene            complement(35604..36959)
                     /gene="mepA"
                     /locus_tag="NBH32_000167"
     CDS             complement(35604..36959)
                     /gene="mepA"
                     /locus_tag="NBH32_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498905.1"
                     /note="MepA (multidrug export protein A), as described
                     originally in Staphylococcus aureus, is a MATE transporter
                     shown to be over-expressed after culturing in the presence
                     of tigecycline, owing to mutation of its repressor MepR,
                     and then to be one of several factors contributing to
                     elevated tigecycline resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MepA"
                     /protein_id="HCD1513808.1"
                     /translation="MKDEQLYYFEKSPVFKAMMHFSLPMMIGTLLSVIYGILNIYVIG
                     FLEDSHMISAISLTLPVFAILMGLGNLFGVGAGTYISRLLGAKDYSKSKFVSSFSIYG
                     GIALGLIVILVTLPFSDQIAAILGARGETLALTSNYLKVMFLSAPFVILFFILEQFAR
                     AIGAPMISMIGMLASVGLNIILDPILIFGFDLNVVGAALGTAISNVAAALFFIVYFMK
                     NSDVVSVNIKLAKPNKEMLSEIFKIGIPAFLMSILMGFTGLVLNLFLAHYGNFAIASY
                     GISFRLVQFPELIIMGLCEGVVPLIAYNFMANKGRMKDVIKTVIMSIGVIFVVCMIAV
                     FTIGHHMVGLFTTDQAIVEMATFILKVTMTSLLLNGIGFLFTGMLQATGQGRGATIMA
                     ILQGAIIIPVLFIMNALFGLTGVIWSLLIAESLCALAAMLIVYLLRDRLTVDTSELIE
                     G"
     gene            complement(37066..37485)
                     /gene="mepR"
                     /locus_tag="NBH32_000168"
     CDS             complement(37066..37485)
                     /gene="mepR"
                     /locus_tag="NBH32_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor MepR"
                     /protein_id="HCD1513809.1"
                     /translation="MEFTYSYLFRMISHEMKQKADQKLEQFDITNEQGHTLGYLYAHQ
                     QDGLTQNDIAKALQRTGPTVSNLLRNLERKKLIYRYVDAQDTRRKNIGLTTSGIKLVE
                     AFTSIFDEMEQTLVSQLSEEENEQMKANLTKMLSSLQ"
     gene            37697..39652
                     /locus_tag="NBH32_000169"
     CDS             37697..39652
                     /locus_tag="NBH32_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1513810.1"
                     /translation="MLSYRQIEILYLLIHKQTFIPINTIANQLGVSPRTIQYDIAYIE
                     QYAETYHYQVSRNKAAGIKVTTAHATLLNELEHNLTNQIHFSKDERLTHIALKLFETT
                     DPVSTKQLAQDVNVSRRTIADDIKMIQAQLDQYHLKLNYVHNKGFNIIGEEDYYRKAY
                     AHFIHQYMKQAAPFIEADIFNSESIALVRRAIIKTLNSENYHLVQSAIDGLIYHILIA
                     IQRLNENFSFDIPINEINKWRHTNQYAIASKMIENLERSCNVTFPESEIIFITLHLLG
                     SKMTEHTASSITFEYHDLSQNIHELITCVSQELGIDMSKDNKLHTSLITHIKPAIHRI
                     KYDMLQPNPLRQEVMRRYPQIIEAVSKHISPIEQDAAIRFNEDELTYITIHFASSIER
                     VATHKQSMIKVVLLCGSGIGTSQLLKSKLNHLYPEFHIWDAYSIYQLEESRLLQDNID
                     YVISTVPCEISAVPVIHVDPFINQQSRQKLNQIINDSREQRVMKMATDGKSLADLLPE
                     HRIIINKQPLSIESAIAVAVQPLINDGIVYSNYTAAILKQFEQFGSYMVISPHIALIH
                     AGTDYVQNGVGFALTYFTEGIIFGSKANDPVHLVITLATDHPNAHLKALGQLSECLSS
                     DLYRQDFLDGNIFKIKQHIALTMTKEA"
     gene            39657..40100
                     /locus_tag="NBH32_000170"
     CDS             39657..40100
                     /locus_tag="NBH32_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1513811.1"
                     /translation="MSLDILSTTRIIVKEQVNDWTEAITIASQPLLQEQIIEQGYVQA
                     MIDSVNELGPYIVIAPEIAIAHARPNNDVHQVGLSLLKLNQHVAFCDENHYASLIFVL
                     SAIDNHSHLSVLQNLATILGDNQTVQQLLTATNAQDIKNILKEHD"
     gene            40102..40386
                     /locus_tag="NBH32_000171"
     CDS             40102..40386
                     /locus_tag="NBH32_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1513812.1"
                     /translation="MKILVVCGHGLGSSFMVEMNAQEALRQLNAPSDIEVEHSDIMTA
                     SPEMADLFICGRDLAENAERLGDVLVLDNILDKAELQQKLSEKLQQLNMI"
     gene            40401..41747
                     /locus_tag="NBH32_000172"
     CDS             40401..41747
                     /locus_tag="NBH32_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCD1513813.1"
                     /translation="MQAILNFIVDILSQPAILVALIAFIGLIVQKKPAATITSGTIKT
                     ILGFLILSAGADVVVRSLEPFGKIFQHAFGVQGIVPNNEAIVSLALKDFGTTAALIMV
                     CGMIVNILIARFTNLKYIFLTGHHTFYMAAFLAIILTVSHIKGWLTIVIGALVLGLIM
                     AVLPALLQPTMRKITGNDQVALGHFGSISYFAAGAVGQLFKGKSKSTEEIKFPKGLSF
                     LRESTISISITMALLYFIACLFAGVSYVHESISDGQNFIVFSLIQGVTFAAGVFIILT
                     GVRLILAEIVPAFKGISEKLVPNSKPALDCPIVFPYAQNAVLIGFFVSFITGVIGMFI
                     LFLFGGVVILPGVVAHFFLGATAAVFGNARGGIKGAVAGAALNGILITFLPLLFLPFL
                     GELGGAATTFSDTDFLAVGIVFGNAVKYMGLFGAILFIIIVGATAILLKGRQKPQQ"
     gene            complement(41841..42866)
                     /locus_tag="NBH32_000173"
     CDS             complement(41841..42866)
                     /locus_tag="NBH32_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde reductase"
                     /protein_id="HCD1513814.1"
                     /translation="MNNKVLVTGGTGFVDMRIISRLLEQGYEVKTTIRDLSKADKVIK
                     TMQDNGISTERLMFVEADLSQDEHWDEAMKDCKYVLSVASPVFFGKTDDAEVMAKPAI
                     EGIQRILRAAEHAGVKRVVMTANFGAVGFSNKDKNSITNESHWTNQDEPGLSVYEKSK
                     LLAEKAAWDFVDNENTTVEFATINPVAIFGPSLDAHVSGSFHLLENLLNGSMKRVPQI
                     PLNVVDVRDVAELHILAMTNEQANGKRFIATADGQINLLEIAKLIKEKRPEIAQKVAT
                     KKLPDFILSLGAKFNHQAKEGKLLLDMNRNVSNERAKTLLGWEPIATQEEAILAAIDS
                     MVKFHLI"
     gene            complement(43182..44204)
                     /locus_tag="NBH32_000174"
     CDS             complement(43182..44204)
                     /locus_tag="NBH32_000174"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD1513815.1"
                     /translation="MYLIEPIRNGEYITDGAIALAMQVYVNQNIFLDDDILFPYYCDP
                     KVEIGRFQNTAIEVNQDYIDKHSIQVVRRDTGGGAVYVDKGAVNMCCILEQDTSIYGD
                     FQRFYQPAIKALHTLGARDVIQSGRNDLTLNGKKVSGAAMTLMNNRIYGGYSLLLDVN
                     YEAMDKVLKPNRKKIASKGIKSVRARVGHLREALDEKYRGITIEEFKNLMVTQILGID
                     DIKEAKRYELSEADWEAIDELADKKYKNWDWNYGKSPKYEYNRSERLSSGTVDITISV
                     EQNRIADCRIFGDFFGQGDIKDVEQALQGTKMTREDLTHQLKQLDIVYYFGNVTVESL
                     VEMILS"
     gene            complement(44182..45126)
                     /locus_tag="NBH32_000175"
     CDS             complement(44182..45126)
                     /locus_tag="NBH32_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacetylase"
                     /protein_id="HCD1513816.1"
                     /translation="MQSSKWNAMSLLMDDKTKQAEVLRTAIDEADAIVIGIGAGMSAS
                     DGFTYVGERFTENFPDFIEKYRFFDMLQASLHPYGSWQEYWAFESRFITLNYLDQPVG
                     QSYLALKSLVEGKQYHIITTNADNAFDVADYDMTHVFHIQGEYILQQCSQHCHAQTYR
                     NDDLIRKMVVAQQDMLIPWEMIPRCPKCDAPMEVNKRKAEVGMVEDAEFHAQLQRYNA
                     FLEQHQDDKVLYLEIGIGFTTPQFVKHPFQRMTRKNENALYMTMNKKAYRIPNSIQER
                     TIHLTDDISTLITTALRNDSTTKNNNIGETEDVLNRTD"
     gene            complement(45116..45916)
                     /locus_tag="NBH32_000176"
     CDS             complement(45116..45916)
                     /locus_tag="NBH32_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-ADP-ribose hydrolase"
                     /protein_id="HCD1513817.1"
                     /translation="METLKSNKARLEYLINDMRRERNDNDVLVMPSSFEDLWELYRGL
                     ANVRPALPVSDEYLAVQDAMLSDLNRQHVTDLKDLKPIKGDNIFVWQGDITTLKIDAI
                     VNAANSRFLGCMQANHDCIDNIIHTKAGVQVRLDCAEIIRQQGRNEGVGKAKITRGYN
                     LPAKYIIHTVGPQIRRLPVSKMNHDLLAKCYLSCLKLADQHSLNHVAFCCISTGVFAF
                     PQDEAAEIAVRTVESYLKETNSTLKVVFNVFTDKDLQLYKEALNRDAE"
     gene            complement(45917..46249)
                     /locus_tag="NBH32_000177"
     CDS             complement(45917..46249)
                     /locus_tag="NBH32_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HCD1513818.1"
                     /translation="MKKLANYLWVEKVGDLYVFSMTPELQDDIGTVGYVEFVSPDEVK
                     VDDEIVSIEASKTVIDVQTPLSGTIIERNTKAEEEPTILNSEKPEENWLFKLDDVDKE
                     AFLALPEA"
     gene            complement(46283..47284)
                     /locus_tag="NBH32_000178"
     CDS             complement(46283..47284)
                     /locus_tag="NBH32_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1513819.1"
                     /translation="MVKLSVLDYALIDEGKDAQKALQDSVTLAKLADRLGFKRIWFTE
                     HHNVPAFACSSPELLMMHTLAQTNHIRVGSGGVMLPHYRPYKIAEHFRMMAALYPNRI
                     DLGIGNNPGTTMVKQALDGINPTYDSYDESISLLRDYLTIKDKPSAHTLGVQPHIDHF
                     PEMWLLSSSATSAKIAAELGIGLSVGTFLLPDINAIHAAKDNIDIYKKHFQASTIKMD
                     AKVMASVFVIVADNEAEVAALQHALDVWLLGKLQFAEFEHFPSVDTAQKYKLNDRDKE
                     MIQAHQARIIAGTQEQVRAQLDDFIATFEVDEVLVAPLIPGIEQRCKTLKLLAEIYL"
     gene            complement(47398..47496)
                     /locus_tag="NBH32_000179"
     CDS             complement(47398..47496)
                     /locus_tag="NBH32_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513820.1"
                     /translation="MESIFKIKIELMNVICRSENMNMKKKNTSEKI"
     gene            47592..48791
                     /locus_tag="NBH32_000180"
     CDS             47592..48791
                     /locus_tag="NBH32_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1513821.1"
                     /translation="MYRYKPLLQSIHLPNGIKISNRFVLSPMTVNASTKEGYITKADL
                     AYAARRSNSAGMQVTGAAYIEPYGQLFEYGFNIDHDACIPGLTNMASTMKQHGSLAII
                     QLAHAGRFSNQAILNFGKVYGPSPMTLHSPIEHDVIAMSHEKINSIIQQYRDATLRAI
                     KAGFDGVEISIAQRLLIQTFFSTFSNKRTDHYGADSLKNRARLCLEVMCAVQEVIDKE
                     APDNFILGFRATPEETRGSDLGYTIDEFNQLIDWVMDVSNIQYLAIASWGRHIYQNTS
                     RTPGEHFGRPVNQIVYEHLAGRIPLIASGGINSPESALDALQHADMVGMSSPFVTEPD
                     FVHKLAEQRPHDINLEFSMADLEDLAIPHAAFKDIVKMMDYGEGLKKHTRDALRQLEQ
                     NYHDSSS"
     gene            48864..49691
                     /locus_tag="NBH32_000181"
     CDS             48864..49691
                     /locus_tag="NBH32_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1513822.1"
                     /translation="MTEIKDKVITKDAFELPYTIIKAKHQPTKGAIVYIHGGGLMFGK
                     ANDLSPQYIDILTEHYDLIQLSYRLLPEVSLDCIIEDVYASFDAIQSQYSNCPIFTFG
                     RSSGAYLSLLIARDRDIDGVIDFYGYSRINTEPFKTTNSYYAKIAQSVNETMIAQLTS
                     PTPVVQDQIAQRFLIYVYARGTGKWINMINIADYTDSKYNIAPDELKTLPPVFIAHCN
                     GDYDVPVEESEHIMNHVPHSTSERVNKNEHDFDRRPNDEAITIYRKVVDFLNAITMV"
     gene            complement(49933..52008)
                     /gene="lip2"
                     /locus_tag="NBH32_000182"
     CDS             complement(49933..52008)
                     /gene="lip2"
                     /locus_tag="NBH32_000182"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip2/Geh"
                     /protein_id="HCD1513823.1"
                     /translation="MLRGQEERKYSIRKYSIGVVSVLAATMFIVSSHEAQASEKTPTS
                     NAAAQKETLNQPGEQGNTITSHQMQSGKQLDDMHKENGKSGTVTEGKDTLQSSKHQST
                     QNSKTIRTQNDNQVKQDSERQGSKQSHQNNAINKTERQNDQVQNTHHAERNGSQSTTS
                     QSNDVDKSQPSIPAQKVLPHHDKAAPTSTTPPSNDKTAPKSTKAQDATTDKHPNQQDT
                     HQPAHQIIDATQDDTVRQSEQKPQVGDLSKHIDGQNSSEKPTDKNTDNKQLIKDALQA
                     PKTRSTTNAAADAKKIRPLKANQVQPLNKYPVVFVHGFLGLVGDNAPALYPNYWGGNK
                     FKVIEELRKQGYNVHQASVSAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGK
                     TYKGIMPNWEPGKKVHLVGHSMGGQTIRLMEEFLRNGNKEEIAYHKAHGGEISPLFTG
                     GHNNMVASITTLATPHNGSQAADKFGNTEAVRKIMFALNRFMGNKYSNIDLGLTQWGF
                     KQLPNESYIDYIKRVSKSKIWTSDDNAAYDLTLDGSAKLNNMTSMNPNITYTTYTGVS
                     SHTGPLGYENPDLGTFFLMDTTSRIIGHDAREEWRKNDGVVPVISSLHPSNQPFINVT
                     NDEPATRRGIWQVKPIIQGWDHVDFIGVDFLDFKRKGAELANFYTGIINDLLRVEATE
                     GKGTQLKAS"
BASE COUNT        16538 a         9938 c         7933 g        17741 t
ORIGIN      
        1 ttttaaatca agaagcgagg ttaatgaaca atgaatatgc atattttata taacttacga
       61 actaaacata atttagaaat tgacgaatta gcacaacaat taaatgagaa atatggtact
      121 aaatatgaag cacaccaaat ttgggaatgg gagaatcatc accatgaacc taaatttaaa
      181 gatgccatgc atttagctga cttctttgat gtaccatatg aaatgttttt agaaagtaag
      241 gttaaagaat atcagaaaca tttagaagaa gtcgatattc gcatggataa gtagatgcaa
      301 ttaaaccctc acaacacgtt aggcatatat cctttcaaat ctatactgga tatatcacat
      361 tgcgttgtga gggtattttt aataattaat atgaattaag acatgttgct atcgttagtg
      421 ttacgacata ttttagtgat cttgctcact ctttaatact ttaccgtttt tagcatcaac
      481 agtaacttct tgttttttat tacctttttt caaatcgata ttgtaaacaa gtttaccatc
      541 atctttttca agtgaccatt ctttaatatc accatcaaat tctttttgtc cttctttaat
      601 ggcttttttg taatctatag catcgctata tttaaagtta tcattttcat tcactgtatc
      661 ttctttttca gtctttttgt taatcacttt tttattttta tcagcaacaa gtacttctga
      721 ctcttcacca gatttttgtt gcgtcacttt ataagcccat tcaccattag aattttcaaa
      781 tgaaattcct ttcaactttt ggcctttgta agtttcttca gcttttttca cagcatcttc
      841 tgggcttgtt ttaacatctt ttaatgcaat aacatctttt gttgtattag tgtcttgatt
      901 agtatttgac tctgttgatt ttgtttcatc ttttggagta tcattgccac atgcagtaag
      961 caccaccgct gacattgata acactgctaa tgattttaat ttcataatat cactctctct
     1021 tcctttttta gaaactcata acaaaagctt atatgctata tagattgtat taccccttgt
     1081 ttttaattct attcataatt attacaaatt tttttaaatt actcttcatg cgttactttc
     1141 gttcgtaatc atttttaaaa tgagtaatgt aatataaggg tgctattatc gactcagttt
     1201 gtctaatgct ttctttggta cttcttcctt ttctacttct tcaaaagttt ctacatgatg
     1261 acctttatgt gtaattttca agtatctatg cggtttaaca tcaaatgtag cagtatacat
     1321 taattgcgtc tctttccctt ctttattaaa agcactttga gtataacttc ggagttgatc
     1381 atccattcta gtagacacct ctgtcgtttt aacataagaa tcatcttcct ttactaatgg
     1441 gttaaattga tctgttatac catgagagtc tattgttttt aaaatgaata gagcagcata
     1501 agcgccaacg atagtaagtg cgagatatgt tattgttttt aaaatcgttt tcaaaagatc
     1561 acctctgaca aaatataaaa taggatgtct ctgaaaacaa cttacataat tgtaattaaa
     1621 atgtaagtat taggagaaaa tgatggtatt tgaatctgaa agatacgaat cgtttttaaa
     1681 caaagttatt ttatagagac cttattatgg aattgaaaga agttaatttt aaggttgtcg
     1741 aatataaaaa catattgatt aaccataaaa gagaaattat ttttgtagat ttcggcataa
     1801 acgtcattaa agaattttct atatgatggt gctgatcata acaaagaaag ctaggcacct
     1861 ttagatacct aactttcaaa gtattagcga taaaacactg tatatactta tttaacttaa
     1921 tttcaaactt gttcaatgtc attaacactt ttcacaagat cgaaagttac attttttata
     1981 tcggctgtat taatttttac attttcccta tctttttgta gctctttatg cgactctaat
     2041 gtatatttac caccatcttt tgtatttatg acgatgttac ctttaggcaa atgttctccc
     2101 atatataagc cataagaaat atgagcaaat cttatgatat gatccaattc ttttaaagtg
     2161 acaacttctt tattaagagt aatgttcctc ttaggtgtat atactctatc agcaactgtg
     2221 taagtccctt ctaaatgtat acgaacttga ttttcattta aaggtctctc tgaataaacc
     2281 caattcctag atttattaga ttttccattt attgtatttt tgctgtatct atcgtacagt
     2341 tttttaacca aagctttttc tgaattattt ggtacttctt caactttttg gaattgttta
     2401 tcttgtaaac ttgaggaatt ttgtgttgac gcgcctgctt ttgaatatat tccaaatgat
     2461 cccgaataga tgatacctag cgatagaata gattttaata agattgaatt tttagaatga
     2521 tgttttttga acatatttaa ttacctcctt gatgtaaagc tttatttgct acaattatag
     2581 aaataataga cgtgttcatg aattaacttc atcttaactc ttgattaact ttaatttgct
     2641 accactctga atttaataac tataaatcgt ctacacataa ttggactaaa tctaagagaa
     2701 tagaatttgt taatttaaaa tagtaagcaa ttcaaagtta tatgtgtaat agataaaata
     2761 gatatcccta tagtgatgcg ttactagcta aacgtaataa cactttagaa aataatgaag
     2821 ataattaagt taaggggtta ctggattgtt tcgactatgt aattaagtat aaaaaaacgt
     2881 ccaacgacaa aacgtaatta taaaaaggta aaatctatag ttcagtttta aattgataat
     2941 ggcatgcgta tcggtgaact atttgcaata aataaagaaa atataaacta tcaagataaa
     3001 acgctagata tcgacggtaa agttaattgg gaaactgatt aaaaatggaa gcatccggag
     3061 taaaagagac aactgaaaga agtaatagct ataaggccat agggctcact acccaaagca
     3121 tcgacttact aagaacactt atgcttggaa tgataagttt attgatagag agtacatatt
     3181 cacaaatacg gctggtagcc ctatcgactc gaacaaaatt agccacatta ctaaaggggg
     3241 ggctatcaaa gagacaactg agattagttc tattaagaaa cctgcaacga cgcatacatt
     3301 acatcattct catatatcta cacttgctca attaggaatt aacttaaaag caatgcaaga
     3361 gcatgtaggt cattcagatt ataaaaactc tagagatata cacacatgtt actaatcaga
     3421 tggcgaaaga tatgatgaat aaatttgaac gattggggag ttaatattgg aaaaagatga
     3481 tacactagca gaaattaagc ctatgctcaa ttttgatgag caaatagcga aattaaaaca
     3541 gatgaatata ttttttaata ttattgacac cgaaaaagca aatgaaattc ttagaaaaaa
     3601 taattacttc ttcaaactag cttatttccg aaaaaatttc gaaaaaaaga atggcggcta
     3661 tttcatagaa tttgcttatt tatcagattt agcaactata gatatgaaat taagatacac
     3721 aatgttgcat ttaactttag atattgaaca tagtttaaag tgtctagtct taaaactaat
     3781 aacagaaaat aaccaagaag atggttataa aataatagat gagttcttat gtattgataa
     3841 atcatataac aattcaaatt ttgacacaaa ttcaagaaca ccagaagaag ttatggaaac
     3901 caaaatcaaa aataaaaacg aaatatttaa gcatatgaat aaaagagggc aactacccgc
     3961 aaagttgaat aaatactatc aaaatccacc cgcatgggtt tgcattgaat tcatgcaact
     4021 aggtcaattc gtttcgtttc tcaacttcta ttacaagaag tacaatgacg aagaattgag
     4081 agttgctaat attttaatgc ctttagttaa aaatataaga aacaaatcag ctcataacca
     4141 acccatcata gcaaatctaa attatgacag tagatctcct caatatttat ttgaaaaagg
     4201 gaataatata ggcatatcta gaaacatgtt cggaataaaa aatttcatag atactttcgc
     4261 tactctagaa ttacataatc aagtttgtag taatgcaatt atccaagcaa gatatcacga
     4321 tttggaccaa cttcaaaagc gatataaaag aaacgaaagc tattataata atgcattagc
     4381 tatcaaaaga ttttttatag ctttagataa aattattgac ttcaacagac caaaagtata
     4441 aactatctag tgaggaaaga gacttatagg tctcgcgagt tattttaatt cgtatgcaag
     4501 aaaaagaaga gctatgcatt ttatttaaaa tgcgtagttc tttttttatg catctaaatt
     4561 catattattt ttgcaatata aacatatctt tgtgcaaatc ccgaacacaa aacattcaca
     4621 tcgtcctttt tctgcccttt ttctatatcc caaaacacaa aaacccccgt aagcctatgc
     4681 ctacgggggt tataatatat taaatattat tgttcttctt taacatatgg taataatgcc
     4741 atatgacgag aacgtttgat agctgtagtc aacatacgtt gatatttagc tgaagtacca
     4801 gttacacgac gtggtaaaat tttaccgcgt tctgagataa aacgttttaa taattcagtg
     4861 tctttgtagt cgatatgtgt aataccattt gctgtgaaat agcatacttt tttacgacga
     4921 cgtccgcctc ttcttggtcc acctgccatg attgtgtgcc tcctttgata attttttcgc
     4981 tttaatttcg ttaattttta ttagaatggt aagtcatcat cacttatatc aatcggtccg
     5041 tttgcatttg caaatggatt atcagattgt ttcgtgtttg atgaattatt gtacgaattg
     5101 ttttgccctg attgttgacc accgaatcct tgaccgtaat cttggaattc attttgttga
     5161 cgttggccac cattttgttg cgcattttta ggttcaagga attgaacgct atcacacaca
     5221 acttcagtaa caaacacacg acgaccttct tgattttcat aattacggga ttgtaagcga
     5281 ccatctacac cagctaaact acctttagat aaatagttat ttacattatc tgcttgtctt
     5341 ctaaaaacaa cacagttaat aaaatctgct tcgcgctccc cttgagcatt cgtgaacgta
     5401 cgatttactg caagagtgaa tgtcgctaca ctcacacctg agggagtggt tctgtattcc
     5461 ggatctttcg ttaaacgacc tactaataca actctattta gcatttaaac gccccctcta
     5521 attattactt gtcttcgtct tcacgaataa ccatgtaacg aatgatatcg tcactgattt
     5581 tagctagacg ttggaattcg tcagtagctt tattgttatc agatttaaca cgtacgatgt
     5641 tgtagaaacc atctttgaaa tcattgattt cataagctag gcgacgttta ccccagtctt
     5701 ttgcttctaa aacttctgca ccttcagtag ctaagatacc gttgaaacgt tcaactaacg
     5761 cttttttagc atcttcctca atgtttgggc gtacgatgta cataacttca tatgttctca
     5821 ttttatattt gcacctcctt gtggtctata cggcttatca atcttaaaac agataagcaa
     5881 ggaataattt tcattactca caataaagaa ttatatcatg cgccattact ttttacaata
     5941 ataattcaaa ctactcttca tatcattttt gacattaata catttgaaat tttctatgat
     6001 attttttaaa aatacacttc acaaaagcga acacatgttc tataatggtt gtgaggtggt
     6061 aaggaatgaa tttaaatatt gattggtcta aagattttca agaattccaa gagatactta
     6121 atagtggcat tcatcctgaa tggctttatt gtgcaaaggc taatcttgtt ttagagcctg
     6181 cttatactgg cgaaggcaaa caatttttca gcactcaaga tattattaac gcgagtaaaa
     6241 ttattccatt cttttaataa ataacacgca taattaaaag aggcgaacaa ttgattccgc
     6301 ctcttctaca tatatactat ttaattaact tcactctatc gtttatacat taaatctgaa
     6361 atgaacgata tcgccatctt gcataatata ttctttacct tctaatcgtt gtctgcccgc
     6421 ttctttagcg ccactttcgc caccatattg tacatagtcg tcataacttg ttacttcggc
     6481 acggataaat ccacgttcaa aatcagtatg aatgatacca gcacattgag gtgcagtcat
     6541 accttgttta aatgtccaag cacgtacttc ttgcacacca gcagtaaaat atgttgataa
     6601 tcctaataat tcataagttg ttctaattaa tcgatctaat cctggttctt caatacctaa
     6661 atcttctaag aacatttctt tatcttcatc atctaaagta gcaatttctt cttcaatttt
     6721 tgcactaata acaatcactt cagagtcttc ttgcgctgca tattcacgaa tcgcttttac
     6781 tttatcatta tcatcatcac caatttcatc ttcaccaaca ttagcgatat aaagcatttt
     6841 tttagaagtc agtaattgcg cttggttcac ccatttttga tcttcttcat taaagtcaat
     6901 actgcgggca ggtttaccat tttctaaagc ttctttaata gtatttaaaa tgcgcacttc
     6961 catctcagca gtcttatctt tttgacgtgc caatttttca attctaggta agcgtttttc
     7021 aactgattct aagtctgcaa gtactaattc catattgata acttcgatgt catcaatagg
     7081 gtctactcga ccagcaacat gagttacgtt atcatcatca aatgcacgaa cgacctgaca
     7141 aatcgcatct acttctctaa tatgtgataa gaatttatta cctaaacctt cacctttaga
     7201 agcaccttta acaattccgg caatatctgt aaattcaaat gtagtaggta atgttttttt
     7261 aggttgaacc atttcttcta atttaagtag tcttgcatct ggcacttcta caatacccac
     7321 gtttgggtct atagttgcga acggatagtt tgctgccaag gcacccgcct ttgtaattgc
     7381 attaaataat gttgatttac caacgtttgg taagccaacg atacctgctg ttaaagccat
     7441 gaatcattct cctatctttg tgtatcatcc tgagatacga tgattttttt tagtttttta
     7501 tcaaacgttt gacgtggaat cataatgctt cgttgacaat tttcacactt aattctaatg
     7561 tcagcaccca ttctaataat tttaaaacga ttggttccac acgcatgttg ttttttcatt
     7621 tctactatat catttattcc atattttgac gccattaaat atgacctcca tgtattataa
     7681 actactaaac accgttttca ctatgattaa aaggtgtcat tattggttgt ggtgtcttga
     7741 taccttcttg taaaaacatt ttttgtactt ctttacgaat aatacgcgca ccagagaaac
     7801 cttcacctgg tattgtttct gctgaaaccc ttaatatcac tctagtatct tcaaaggcat
     7861 caataccaat cacaacagga tcacttacaa ataaataata tttactacgc agagatgtaa
     7921 ataatttgtt taatttcttc tcaacattat caatattttc atctacagat actggaattt
     7981 taacaattgc tgttccattc gtaattgagt aattcgttat ttcacccatg ctactatttg
     8041 gcaagattgt aagttctcca gatattgtat taatacgtgt tgaacggagc ccaattgatt
     8101 taactgttcc ctctgctaca gttgtaccac cgttgtttat cttaacataa tcacctacat
     8161 caaactggtt ttcaaatata ataaagaaac ctgtaattac gtctttaact atagtttgag
     8221 caccaaaacc tactgctaag cctacgacac cagcacttgc aattactcct tcaacactaa
     8281 tgccaaattt acttaaaatg gtagttacaa ctataaacca cacaatatat ttaacaacat
     8341 tttgcaccaa tgatattaat gttttcgaac gttttttatt acccttttta ctcttgtttt
     8401 gtattttaaa tccttgggca atcattttat caagtatttt aattacgatt aatgcaacga
     8461 taatatatat aattatcatc gctaatttag tagcgacact ttcatatgtt tcaattttag
     8521 ttaatggttc gaatagcgat gaaataatac tcataacttg atccataatt agcgttactc
     8581 ccttctatat agtctcaaat aattttatta atattgctgt attcttccaa cctactgaag
     8641 tcgcatcatc ttcaaagaat atagataact atcgatatat ctatagattc acaaagtgat
     8701 attatataaa attgtgtaac tatttaccat acttacgatt taattgttca atgattctca
     8761 caaagtcatc ttgtgattca aactcaaatg agattttgcc gactgatttt tttatcgata
     8821 tatctacttt tgcgccatac tgttctcgca actgtcgttc ttgttgcttt ataaatttag
     8881 gcttagttat atctactttg tctgtttccg actttgacga aacatttttt aattcattaa
     8941 catggttttc taaatatctg acactccact tttctttaac aacccgtttc gctaacctaa
     9001 gcatttgttg ttcatcttta attgccaata acgttcgtcc atgtgcactt gtcagtcgcc
     9061 catcttttac catgtcagca atctttttcg gcaaatgtaa taacctcaac atattcgcta
     9121 tatacgggcg cgacttactc aatcgcttcg ctacttcttg ttgtgtaatt ttcaaatctg
     9181 tcatcaaacg ttgataactt tcagcttctt caatcgcatt taagtcttct cgttgtaaat
     9241 tttctatgac cgccagttcc atcatatctt catctgttaa atctttgata atcgctgata
     9301 cgtattttag accagcaatt ttcgaagctc taaaccttct ttcaccaact acaatgtaat
     9361 aaccttgaac tgtttttcta agcacaattg gttgcaaaat accatattgc ttaattgaat
     9421 ctgccaaatc atttaaatga ttttcatcga aagtttttcg gggctgatac ggattcggtt
     9481 tgatatccga tatatctatt tgcttaattt gttcatattt tgttttagta atacgttgat
     9541 cttcactttt tgacaattca ctcacatcac tttttatttg ataacaacag ttaaatatat
     9601 tttatgtatt ctaaatgagt cagacaacca ttaaccacac tttatttaaa aaataattca
     9661 ttttttaact tcaagatacc aaatacaatt tcacaaacaa attaactttt aaatcaaaat
     9721 tttctgaata attgttgaca aatctttttt tactctgtaa gctaattaat atcttataac
     9781 agtttaatga aacgtaaaca caataaagag gaaagtaaaa cacaccctgc ttatacagag
     9841 agtctttagt agctgagaga agattttgaa agcgtgtttg aaaatggcct tggagtgttg
     9901 atgccaatat gaggtgtcta cgggttcgcc cgttatagcg atacagtatt aacattgatg
     9961 ttaaatggcg tactggattc tttacgcacg attttttgtt aataagtatg agatagcaca
    10021 ttactatatc cttacttaac gacttaaatt gtgataattg ttcagtaagc atatttactt
    10081 ttaatgcgta ctgaataagg ttatttcagc gatggaataa caaacaaagg tggtaccgcg
    10141 aaacataagc tttcgtcctt tttatccgat tcattcgggt acgaaggacg gaagcttttt
    10201 attttttctt ataggagggt cttaatatga aggatacaca gttagcccaa atcacattaa
    10261 ctgatgattc aaccggtgct atagcaaatc caatccattt atctactgcc tacaagcatc
    10321 caaaactagg acaatcgaca ggatttgatt atacacgtac taaaaatcct acacgctcaa
    10381 catttgaaac ctgttttgcc aaacttgagc atggtattgc atcattcgct acatcaagtg
    10441 gaatgtcagc cattcaatta atatgtaatc tatttaaacc tcatgatgaa attttagttt
    10501 cattcgattt atatgggggc acatttagat tgtttgagtt ttacgaacaa caatacgata
    10561 tcaaatttaa gtacgttgat tttacagatt atgaacaagt tgaaaaagaa atcactgata
    10621 aaacagttgc attattcatc gaaccaatat ctaacccaca aatgattgct attgatgtaa
    10681 agacgtacta tcaactttgt aaagctaaag gcttattgtc aattatcgac aatacttttt
    10741 taacacctta tctttcaaca ccgttagcag aaggtgctga tatagtctta cattcagcta
    10801 cgaaatatat tggtggacat aacgatgtac tagcaggtgt cgtaaccgtc aaagatgaat
    10861 cactcgcaca acagttgttt gattttcaca acatgactgg cgcaacacta tcaccaatag
    10921 atagttattt gttgttacgt ggacttaaaa ctttgcattt acgcattgag cgcgcgcaat
    10981 caaacgcaag aaaacttgct aaaaaatgtc agtcacttca agcgattgac gaagtactat
    11041 atagcgggca aactggcatg cttagtttaa gacttaacaa ggcctatagc gtcgctaaat
    11101 tattagaaaa tttagacatt tgtatttttg cagaaagttt aggaggcact gaaacattag
    11161 tgaccttccc ttacacacaa acacatgttg atatgccaga tgctgaaaaa gataaacgtg
    11221 gcattgatga gtatttaatc cgcttatcac taggcgttga aaattatgaa gacatcgaac
    11281 acgatatcat ccaagcatta gataaagctc agattggaga gattgtatga cactttcaaa
    11341 agagacagaa gtgatattcg attggcgtag aggtgtggaa tatcattcag ctaacccacc
    11401 actctatgat tcttcaacat tccatcaaac aagtcttggt ggcgatgtta aatatgatta
    11461 tgcacgaagt ggcaacccta accgtgaact tttagaagag aagttagcac gattagaaca
    11521 gggaaaattc gcttttgctt ttgcatcagg tattgctgct atttcagcag tacttttgac
    11581 tttcaaatct ggtgatcatg tcatcttacc tgatgatgta tatggcggta cttttcgcct
    11641 cactgagcaa attttgaatc gttttaatat tgaatttaca accgtagata ctaccaagat
    11701 cgaacaaatc gagggtgcca ttcaatcaaa cacaaaatta atttatatcg aaacaccatc
    11761 gaatccctgt tttaaaatta ctgatatcaa agctgtttct aaaatagccg aaaagcatga
    11821 actactggta gctgtggaca atacatttat gacaccgtta ggtcaatcac ctttattact
    11881 aggcgctgat attgtcattc atagtgctac aaaattttta agtggacata gcgatttaat
    11941 tgctggtgct gtcattacta ataatgaggc aattagtgag gctctttatt taatacaaaa
    12001 tggtacaggc aatatgttat ctgctcaaga tagctggaca cttgctaaac atttaaaaac
    12061 atttccaatc agatttaaac aatctgtcga aaacgcgcaa aaaatcgtgt catttttaat
    12121 aaagcaagat gaaatttcag aagtttatta cccgggactt actgcatctc atttagaaca
    12181 agctaaaaat ggcggtgccg ttattggctt acgtttagct gatgagtcta aagcacaaca
    12241 atttgtcgat gcactgacat taccactcgt ttcagtgagt ctaggtggtg tggaaacaat
    12301 actttcgcac ccagcaacaa tgtctcacgc aacactacct gaagaagtga gacaagaacg
    12361 tggtatcact ttcggtctat tccgattaag tgttggtctc gaagatcctg atgaactcat
    12421 tgcagacatc aaatacgcat taaaggaggc attcaatgag tcaattcctc acacaattga
    12481 aagataatgt tttagtagct gatggcgcta ttggaaccat tttatactct gaaggattag
    12541 acacctgtcc agaagcatat aatcttagcc atccagataa agttgaacgt atccatcgtt
    12601 catatatcga agctggtgct gatgtcattc aaaccaatac ttatggtgca aattttgaaa
    12661 agttaaaacg attcggtctt gaagataaag ttaaagctat acatcaagct gccgtacgca
    12721 tcgcaaaaaa agcagcaaat aaagatacgt atatattagg cacagttggt gggtttagag
    12781 gtatcaaaca agaggatatc agcttacaaa ctattcttta tcatactgaa attcaaatag
    12841 acaccttaat tgaagaaggc gttgacgcgc tacttttcga aacgtattac gacctagaag
    12901 agctaacaaa tgtcatttca cgaacgagaa agaaatacga cattccaatc attgctcaat
    12961 taactgcttc aaacacaaat tacttagtta atggtcaggc aatcaatgaa ggattaaaac
    13021 aactcgttca atgtggtgca aacatcgtgg gactcaattg tcatcatggc ccacaccata
    13081 tgcaagagtc tttcacacat attgaattac cagagcacgc atacttatct tgttatccaa
    13141 atgccagctt attagatatt gaaaatagta aatttaagta tagtgacaat gcacaatatt
    13201 tcggccaagt tgctcaaaat ttaattcgcg aaggtgttcg tttaattggt ggttgctgtg
    13261 gtacaacgcc agagcacatc aaatttatta aagaatctat tcagacactt aaacctgtta
    13321 atgacaaaaa agtgattccg ataccaacga aagcactttt caatccatct caaaataaag
    13381 ttagacaatc attaacatct aaggttcaag aacgtccaac cgttattatc gaattggaca
    13441 caccgaaaca tttggacacg gatagatttt ttgaaaatat cgctaaactt gataaagcaa
    13501 atgtagatgc ggtaacactc gcagataatt cattggcaac tgtcagaatt agcaacattg
    13561 ctgctgcaag cttaattaag caatattaca atattgaacc actcgtacat attacatgtc
    13621 gagaccgaaa cttaatcggc ttgcagtccc atttacttgg attatcgctc attggcgtta
    13681 acgaaatatt agccataact ggtgatcctt caaaagttgg tcacttacca ggtgcaacca
    13741 atgtctatga tgttaattct aaaggattaa ctgaactcgc tctaagattt aatcaaggta
    13801 ttaacactga cggtgatgcg ctgaagaaac gtacacactt caacatcgct ggcgccttta
    13861 accctaatgt tcgtaaatta gatggtgccg taaaacgact agagaaaaag atagaaagcg
    13921 gaatgtctta ttttataaca caacccgtgt acagcaaaga gaaaatcatt gaaatttacc
    13981 atgccactaa gcacttgaac aaaccatttt tcataggcat tatgcctatc gcaagttaca
    14041 aaaacgcact ctttttgcat aatgaagtgc caggaatcaa gatgtcagat gaaattttac
    14101 aacaatttga agcagttaaa gatgataaag ccaaaacacg agaactaagt cttaagcttt
    14161 caaaggattt aatcgatact gttcatgaat attttaatgg tttatacatt atcacaccgt
    14221 ttcaaaatgt cgaagattca ttagaacttg cagcatactc aaaatctatt actgctcaca
    14281 aggaggcaat attatgacaa caattaaaac atcaaactta ggattcccaa gattaggtag
    14341 aaaaagagaa tggaaaaaag ccatcgaaag ttattgggca aagaagattt ctaaagaaga
    14401 attagatcaa acattaactg atctacataa agaaaattta ttattacaaa agtactatca
    14461 cttagatagc atcccagttg gtgatttctc tttatatgac catattttag atacgtcatt
    14521 attattcaac atcatccctg aacgtttcca aggtagaact atcgatgatg acttattatt
    14581 tgatattgct cgcggtaata aagaccatgt tgcaagtgca cttataaaat ggtttaacac
    14641 taactatcat tacatcgtac ctgaatggga caatgttgag cctaaagtga gtcgcaacgt
    14701 attattagat cgctttaaat atgcccaatc tttaaacgtt aacgctcacc ctgtcattgt
    14761 tggtccgatt acttttgtta aattatcaaa aggcggtcac caaacttttg aagaaaaagt
    14821 taaaacgtta ttaccacttt ataaagaagt gtttgaatca ttaatagatg caggtgctga
    14881 atacattcaa gttgatgagc caatcttagt tacagacgac agtgaaagct atgaaaatat
    14941 tacacgtgaa gcttatgact atttcgaaga agctggtatt gctaaaaaat tagtcattca
    15001 aacatacttc gaacatgcac atcttaaatt cttaagttct ttacctgttg gtggtttagg
    15061 tttagacttt gtccatgata atggctataa ccttaaacaa attgaagctg gagattttga
    15121 taaatcaaaa acattatacg ctggaattat tgatggtcgt aatgtatggg caagtgacat
    15181 tgaagctaaa aaagtcttaa tcgataaatt gttagcacac actaatgaac ttgtcattca
    15241 accatcatct tcattattac atgttccagt atctttagat gatgaaacat tagatacaag
    15301 tgttggcgaa ggcttaagct ttgcaactga aaaattagac gaattagatg cattgcgtcg
    15361 cctatttaat caaaacgata gtgttaaata tgataaatta aaagcacgtt atgagcgttt
    15421 ccaaaatcaa tcattcaaaa acttagatta tgatttcgaa agcgtgcgta cttcgagaca
    15481 atcaccattc gcgcaacgta ttgaacaaca acaaaaacgt ttgaacttac cggatttacc
    15541 aacaacaact attggatcat tcccacaaag ccgagaagtt cgaaaatacc gtgcagattg
    15601 gaagaacaaa cgcattacag acgaagcata tgaaacattc ttaaaaaatg aaattgctcg
    15661 atggattaaa attcaagaag acatcggctt agatgtatta gttcacggtg aatttgaacg
    15721 taatgacatg gttgaattct tcggagaaaa attacaaggt ttcttagtaa ctaaattcgg
    15781 ttgggtgcaa tcatatggtt cacgtgccgt aaaaccacca atcatttatg gtgatgtaaa
    15841 atggacagcg cctttaactg ttgatgaaac agtttatgca caaagcttaa cagataaacc
    15901 agttaaaggt atgttaactg gacctgtaac aattctaaac tggtcatttg aacgtgttga
    15961 tttaccacgt aaagtcgttc aagatcaaat tgctttagca atcaacgaag aagtattagc
    16021 acttgaagct gcaggaatca aagttatcca agttgacgaa cctgcattac gtgaaggctt
    16081 accattacgc tctgaatatc acgaacaata tcttaaagat gctgttttat catttaaact
    16141 tgcaacgtct tcagttcgtg atgaaactca aatccataca catatgtgtt attctcaatt
    16201 cggtcaaatc attcatgcta ttcatgactt agatgctgat gttatttcaa ttgaaacatc
    16261 tcgtagccat ggtgatttaa ttaaagactt tgaagatatt aattatgatt taggtattgg
    16321 tctaggtgta tatgatattc atagtccacg tattccaaca aaagaagaaa ttactacagc
    16381 aatcaatcgt tcactacaac aaatcgaccg ctcattattc tgggtaaacc ctgactgtgg
    16441 tttgaaaacg cgaaaagaag aagaagttaa agatgcattg actgtacttg tgaatgctgt
    16501 taaagctaaa cgccaagaat aatcgcagca tttatcatat aataaactaa aaacgggtga
    16561 ttttatgaca caatatcctt tatggcaaca attaaatact ttaaaacaag caacttgggt
    16621 tgatttaacg catacatttg atccagaaat accacgtttt agtgagtttg aaaaaggcga
    16681 ggtttcaact ttattcactg ttaaagatca tggtttctat gtgcaacgtt ggaatattgt
    16741 aacgcaatat ggtactcaca ttgatgcacc aattcacttt gtcgagaata aacgttattt
    16801 agaagacatt gatttaaaag aattggtatt gccgttaatt gtattagact tttcaacaga
    16861 agtagcgaac aacaatgatt tcattgtaac acgtgcgcat atagaagcat gggaaaaaga
    16921 gcatggtact attgaacctg gcacttttgt tgcacttcgt accgattggt caaaacgttg
    16981 gcctaatatt gaaacgtttg aaaacaaaga tgcgaacgga caacaacatg caccgggttg
    17041 gggattagat gctttgaagt acttaattga agaacgccgt gttgaagctg ttggtcatga
    17101 aacattcgat accgatgcat ctgtggacgt tgtaaaaaat ggtgatttag ttggcgaacg
    17161 ttatattctt ggtcaggata aataccaagt cgagttatta actaatttag atcaattacc
    17221 aactcgtggc gctatcattt acgctatcag ccctaaacca aaagatgcac ctgggttccc
    17281 tgttagagca tttgcaatta aaccatcaaa tgactaatat tacccaataa atccataaac
    17341 aaataaaaac aggttatacc aacatgtaat taatactgtt gtgataacct gttttcttat
    17401 tatttatact ttgatacaac tgcagcgata gcttgaccgc caccaataca caatgacgtt
    17461 aaaccagttt caacttcatc attcagttga tgcaataatg tcactaatac tctagcacca
    17521 gatgcaccaa tagggtgtcc taatgcaata gcgccacctt taacattcac cttttcagga
    17581 ggtaatttta attcgcgatc tacagctaat gattgtgctg caaatgcttc atttaattca
    17641 aatacatcaa tatcgcttaa ttctttttta ctacgtttca aagccttttc aacagcgcca
    17701 actggtgcaa tacccataat agaaggatct acaccatgac ttccaaagcc atcaagcact
    17761 gccaatggtt cgatatttaa ttctttagct ttgtcttctg acatgactaa catcatcgca
    17821 gcaccatcat tgattcctga tgcattacct gctgtaactg taccgtcttt tttgaaagct
    17881 ggtcttaagc gacttaattt ttcgactgat acattttcac gtacaccttc atccttagtg
    17941 acaacgattg gttcaccttt acgttgagga atcgatactg gaactatttc actatcaaat
    18001 tcaccatttt gctgtgcacg tactgctttt tgttgtgagt ttacagcaaa tgtatcttgt
    18061 tcttctcttg aaataccata ttgctctact aaattttcag cagtaatacc catatgatat
    18121 tgattaaata catctgttaa accatcatat accatgctat caaccattga ttgatgtccc
    18181 attttaaaac caaagcgact gttgttgaca agcattggtg attgagacat attctccata
    18241 ccgccagcta gcacgatgtc attttcacca gtcacaatag attgatatgc taattgaatc
    18301 gactttaacc cagaaccaca tactttattc actgtaaatg caggtactgt ctctggtaag
    18361 ccacctttca tagcagcaat tcgtgctgga ttttgtcctt gtcctgcttg tagtacgtta
    18421 ccgatgataa cttcatcaat ctcacttgga ttcaaacccg tctctttaat aatatgttct
    18481 attaaagtcg cacctaaatc ataggctggc acgtctttaa acgcacctcc aaaaacgcca
    18541 ataggtgtcc tgtatgctgc tgctaatacg actctcgtca tgtcatattc atctccttat
    18601 gatatataca attttattac taatacgtct aatgaaccaa tacgccacta acatccaatt
    18661 tatgaaacca attgctacgt atgttacatt ttcattcttg ttatgcattt aacaaatcat
    18721 attatacaat gatgtaattt gtttgggcaa tcggaaacgt aaacggtttc actttaacat
    18781 ttcacactct taaatatgct ctatcatcct catctatagt acaaaattat gctttgattt
    18841 accacaaatt atcattaatc atttcgcgta atgactctaa ttaaatgcta tctatcattg
    18901 catcgctatt cacagctggt ttcaccatca atttctcatc aacttttcct taccattacg
    18961 tctctagtca aatattaaat cgctgatgat tgttaattcc gcaattacct tgcagtcgcc
    19021 cctccttcca cttcatcgcc aaatcttcct ctattcacta gaaaaaataa atagtatact
    19081 tagtaaatta attacacatc ttaaaggagt taaaatttta tggttaatgc attaacacca
    19141 aaacatttag ctgaaaagcg tgcaggattc catgagttgt tctttgattt aattttcgtc
    19201 tatgccattc aaaaaatcgc acatgtgatt ttaacaacac aaaacggttc catttcagct
    19261 gatttatttt tcaaatacat cgtcatgagt ttgttcttat ggttaatgtg gtctcatcaa
    19321 acatttttta caaatcgatt tggtcaagtt acattcaaag acgtctcgtt tatgatgttt
    19381 aatatgttta tcatggtctt tctatctaac agcctatatc cggactttga aaagacattc
    19441 ttcccattct tcttatgcgt ggcaattatg tatttaagta tcggattcca gtatttatta
    19501 catatacgta cagggttaga ttatggcgat aagcgtacat gtcaagcttt tgcggccgta
    19561 gcctttgtga tttcattttt atcatttcta tcattagtac taccacaaag tatccattac
    19621 atccctggat ttttaggcgt attcattgca gcgacaggac ttattccatt tcagaaatat
    19681 cttgtattat cacctgttaa tatgatgcat ttagtagaac gtttttcatt actaacaatt
    19741 attatctttg gcgaggtact ggttggttta gcttcgtcat catttagcat tgatcatttt
    19801 agttacattt atatattcca atttatgatt ttaattagtc ttttcggtgt atattggatc
    19861 attactgaaa attacattaa tcataaattg tcatctatcg gtttccgttt atcgtatacg
    19921 catttattaa tcaatatcgc tttaggtgtg attaatgccg caattgtttt tagtaataac
    19981 aataaactaa atgacttgtt tgaaattaat atgatgtata tcagtgtttt aatattctat
    20041 attggattat ggttaatcac gccttatttc cataatgaac tgacaaacgc aaaatatatt
    20101 tcttcttcac ttggcatact tgtagtatca tatatcatca gccttatctt taaagggcac
    20161 gaccaagtaa tgattatctc ggtatccgtt gcgactttct gcatcatgct catctatttt
    20221 aaaaatcaac gtctacgtca aagtgatgaa tagataacct tgatctcgat caatgtattt
    20281 gaactgcatc caaaaatatt gtgcttcaca gtatattttt gagacaatca tcagttatga
    20341 tacatgatcg agtttttata tttccgagga aatcattggc atataccatc atcaattgat
    20401 acacgcaaat aaatttcagc aacatactca ataatatata cacgttaatc ctcactataa
    20461 aaggatgcaa ttcccacaca acctaatctt ctaaaaacaa atgatatgat atagagatgt
    20521 cacctcaacc tatcatctcg catttcattc cacaacacca aaaagagcag atgataatcg
    20581 tattgattta tcacctgctc taaatattat ttcaatgact agcttcacac caagaatatt
    20641 gattatagtt cttgtttgtt aaaaatttta atagacaata tataagaaac acttgttata
    20701 acgataccaa tgaccgcata tactatgaca actagccatc cttcagtgct aattttttca
    20761 aattctaatg ccgatgcacc actaaccata ccatatataa agaaaaagac cacgaaagaa
    20821 ataagtagta gaatagatgc aaatatcata atgacattag agttttcagc gccaaattta
    20881 aatgtcaatg ggaaaatgat agagtatgcc cctatgatac caaaactcat taaacctgac
    20941 ataataccaa tcatcacact ttgggtcaca atcgtagtca caactaatcc aatcattaaa
    21001 cttgcaccga ataagattaa ataaaaggca aagtatgact taatataatc actacgttta
    21061 accggtaaag tagatacata atacatccat cttgagtctt tttcatgttt aatattatca
    21121 gtaataggtg tgattaacat aaccccagcc attgccgaac tcattaacgg attaaatact
    21181 gcaaagtatc ccgcagctat gatagcgact ataaaataaa tatatgtttg cattctcgtt
    21241 gcataaaaac tacttaggaa catacctttc attatacttc acctcgcatt atgattttcg
    21301 tagcgtcatc aatgtgtttt aaaggttctg cattttcaat ttctgcataa tctttaacta
    21361 aaatttgata tttccccttt gacaatctag aagctatgat taaatgctta ggaatttcaa
    21421 catctttatc ttctgtcgta acaattccat attgtgctaa aagtatatct ttctgttcag
    21481 ttaaaatcat tcgtccatct ttcataaaca ctaatttatc cgctaattgt tctatatctt
    21541 cagaaatatg cgatgatatt aagatgcctc caccttgcgc gacaaaatct tctaatagtt
    21601 ccattacttc ttctcgtcca gaaacatcca tacctgcagt tgcttcatct aagattaata
    21661 acttcacatc atgagaaagc gcaattgtta aagctatctt cattcgcatc cctcttgaaa
    21721 aagttttaat tttagtttgt agtggcaact caaaatattt gattaaatca aagaattttt
    21781 gactattcca agtcgtataa atagattgaa atactttatc aatatcttta atagtcaatt
    21841 tattaggtac tctcaaatca tcaaacacta tacctatgtg ttctttatat tcgacatcat
    21901 ccatagcatg ttcttcatca aaaaatgtga tactaccgtt atctttatgt cgattgccta
    21961 ctagaatatt aattaatgtc gatttacctg agccattttt cccaattaat ccaactgtct
    22021 cattagtaga aatcgttaaa gatgcatctt gcaatttaaa atccgaatct ttgtattgtt
    22081 tgttcacact atctacgttt aacaatgttg tcatatccat gctcctcttt gtttaatttt
    22141 aataaaaacg tcttctcttc aataaataac taaaggcgtt atatatgaaa atagcgatga
    22201 taagtagcaa actaaagctt tgattaactc cagtagtaat tgaataaagc ccaattacta
    22261 caatggctag tatcaatatg gataagttga tcgcatatgt tttaaataat gcaattaatt
    22321 ctatatggcg ttcaccttca tccaatattt ccaatcgctt ttcagtgtaa ttcttatctg
    22381 caatttttgg cattctatca tcaaatcttc tattaaacag tgtaaattgt gtattgaaaa
    22441 tagcactagc aaaaaatggt attaaaaaga atagtattgc atttgcttca gcgtgtccta
    22501 ccacaaaaat tagtaacact acaaaggcaa taattgtctg tataatgctt aatatacttc
    22561 catttaaaac ataacgattc gcaagcaatt catattgatc tgttgcatcg atatctacct
    22621 cttcatctac aagccgctta tacttcaatg cacgtctttg attcatcaaa acaatgattt
    22681 ctaccaatat gataataatg gtcgcaacaa ttgatattac aacgacattg ttgtatgtcg
    22741 caaacgtcaa attctcaata cccaatccat caaagttacc taaaattcca cctatgatac
    22801 cacctacaag tccacccagt aaaagatatc cgatatatct tagtatcttc atgcctcatc
    22861 ctcctcaata ataaaaatag tttccacctt ttcattgaag atacgagcaa tctttattgc
    22921 agttaatacc gatggcataa agttgtttcg ctcaattagc gaaatggttt gtcttgaaac
    22981 acccgcttgt ttagcaagct gcgtttggtt taagccatct cgtgctcgta attctttcaa
    23041 tcgattacgc acatcgcatc aactccttaa ttacactgta aattatattt gtctttttga
    23101 caactatatt tgtcaaatct acacaaaaaa atatgagtgt caacctatgt atgacatttt
    23161 gaaacaaaca cctcaacgcc tacaagtcgt atttttttac tttcgttaca ccttgctgca
    23221 taattaatag cattctaatt ttagtatgat gcacgcattt tcactaaatc aaaccattca
    23281 aaggagacta ttatggcatt tacattatct gcaattcaac aagcacatca acaatttact
    23341 ggtgttgatt ttccaaaact attcaaagca tttaaggata tgggtatgac gtacaatatt
    23401 gttaatattc aagatggcac agcaacttac gtacatcaat cagaagatga tattgttact
    23461 tcatctatca aaagtaatca tcctgtcgct ccaagatcaa atcaatctat cgttcaagat
    23521 gtattaacta gacatcaaca aggacaaaca gatttcgaaa cattttgtga tgaaatggct
    23581 gaagctggca tttataaatg gcatatcgat attcaagctg gtacttgtac ttatatcgac
    23641 ttaaaagaac aagctatcat ttcagaatta atacctcagt aaaatgtatt tataacaatt
    23701 ttttaattat tatataaaat attgttgata ataattatcg ttcggtataa agtaaatact
    23761 atatactact tatgatcgag gttgattatc atgataacta acacttttat tttaggcatc
    23821 acaggcccga caagtcttgt cgtcattagc attatcgctt taattatttt tggtccgaaa
    23881 aaattaccac aatttggtcg tgctatcggt tctactttaa aagaatttaa atctgcaaca
    23941 gaagatttag ataaagagtc tcacgataca cccagtaagg aatcgaagca acagcgagag
    24001 caatagtact gatgatacat taatggttca ctttagcgaa ctacgccatc ggttagtaaa
    24061 aattttattg tcgttcgtca ttacggtcat cgtcgtatat gtttcatcat tttggtggat
    24121 gacaccattc ataacgtata ttacccgtgc acatgtgtcc ttacatgcat tttcattcac
    24181 agaaatgatc caaatatacg tgatgattat atttttcatc gcgttttgtt tcatttcacc
    24241 agtcatgttt tatcaattgt gggcattcat tgcacctgga ttacataata atgaacgtca
    24301 atttatttat aaatatagtt ttttcagcgt gttattattc tgtgctggcg tcgcttttgc
    24361 attttatgtt ggctttccaa tgatcattca attcgcgtta aaattatcat taactttaaa
    24421 catttcgcct gtcattggct ttaaagcgta tttagttgaa cttatccgtt ggcttttcag
    24481 ttttggcatt ttattccaat tgccaatatt attcatagga cttgcaaaat tcggtcttat
    24541 agataccaca tcattgaagc attatcgaaa atatatttat ttcgcttgct ttgtgctggc
    24601 gagtatcatt gcaccaccag acataacatt aaatatatta ctcacattgc cactcatatt
    24661 gctctttgag ttcagtatgt tcatcgttaa attcacatgc cgaggcaaac cgccaacaca
    24721 ttaatcttat aagaattaat gctgtgatta tcgttatact gcaataccga gtatttccaa
    24781 catcgcaaca aaaaaaacag gcactcggcc tgttttttat tttttaaatt taaagattaa
    24841 gatagcaact accataatat aagcagcttg tgctatcaat ggttcataac ttggatagaa
    24901 gcccaaccag ttaatcgttg ggaatccttc aataacatgt cttggcatcg cacctaataa
    24961 ttgtaacttt tgaatactta cgccaagcat tttgaatccc ataataaaaa taaagatcga
    25021 caatacccta aatatataga atataggtat caatctgacg ataaatctaa ataatagcgc
    25081 aaagacaatt aatatcacga tggccaagac gatacctatg acaaaatctt tggtcgctaa
    25141 ttcgcctatc attcccatat agaaaatgat aacctcgaca ccttcacgca acacagatat
    25201 taaaccaatc gtcgctaaca ataccaaatt accattacta atcgcattgg catacatatt
    25261 tttaatcata tcattccagc gtttcgcatt tgaacgtttg tgcatccaaa caccaacgat
    25321 aaacattaat atgaccgcaa caatacctaa tcccgcttcc atactttcac gaagaatgcc
    25381 actattacct aaagtttcta caaacgtaat tgctaagata atactcagta caagtccggc
    25441 aattgcacca ccaatcacac ttgcagtacc cttcttatct ttgacattac gcgtcatagt
    25501 agtcaatgtc attacaatta ataacacttc tagaccttca cgtaaaaaga taatcatcac
    25561 atcaacaaag ctataactat ggccaacaac ctctttaatt tggttattta aatctactaa
    25621 gccatctttc acatgtgctt tattatgttc gtctaataca ctttgatagt atggtatttt
    25681 atcttcaatt ttcgtataca aagcaccgtc tttagtttga atttgacctt caacatacgg
    25741 ccaaatttct ataaaatgtg taagcgcagc atcagcatcc gacaattgat tgtcgtcgat
    25801 agctttaatc gccttctcta actcatcatt taattgtgat acatggtatt gatcatttgc
    25861 agacgtacca cttttcttat cgacatgatc aatatttgat ttaaaagttg tccaagcatg
    25921 tgacactttt gtcgtatcta atggcgactt atgaattgca attctaagtt gtaataatgc
    25981 gacttcaatt tgtccatatt gatttgcgtc ataattgcga atcaccgttt cattacttgt
    26041 ccaaatctga ttcaaactat tgttcagcga ttctagctct gctttatttt tatctttaat
    26101 cgcttttgtc atcgctgcat ctttggcatc gacttgctgt tgtaatagtt taattttaga
    26161 acccgcatct ttactagcca atttctcttc ataagcaatt aatgacttcg ttaattgcga
    26221 aagtgtatct ttttgatcat cattcgcttt tgcatcttca agctttctca catctgattt
    26281 gacagcatta ccttcactat tatcttcaag cgataatttc ttaactgcac ttaccacttg
    26341 ctcaattgct ttctgcttat tgtcattcga tatcgaatta ttagaaagta cagatttcgc
    26401 atccgtaatc acactatata catcactaat actttgttgt gctgctgcct gacttttcag
    26461 taacccaaag ctacacacca tagcagcagt tattagcatt gctataattt tagtcaaata
    26521 atgtttcacc gaggtatcct cccttactaa cacctggtaa tactaaaaat gaagcagaac
    26581 ctctatgtgt aatgtattcg tttaatttat cattactgcc taaattattt tgaatatcga
    26641 taaattgttg ggtcgctttt tgaaaagcaa tgaacaacaa gcctgtctcg aagttacctg
    26701 tgcggtcatc cgtaccatca acataattaa aggctctacg taaaattgac gtattagctt
    26761 ctttcgctag tctcgtatga gcatctttat ctataatgta ctcaccgtga ctatctttcg
    26821 cttttaagtc aatttcatca aactctttcc cgcctgttaa cggcgcacca ctatgtcgtt
    26881 tccgaccaaa tgtcgcctct tgttcttcta atgcagtgcg atcccacgtt tcaatgtgta
    26941 tttggatacg tctgacaaca caataagttc catgcttcgc ccatccatca tcaataaaca
    27001 cataatcttt aagttgatta ttcttcctag gattaattgt tccatcttta aatgccatta
    27061 aattccttgg cgtttcctta cctttagcag agataaaacc agattgcgcc caacgcacct
    27121 taacaatatc tcgaaacgga cgaactaaat tatgaaccgc atgaaaagac acttgtgaat
    27181 catttgagca tgcttgaatc ataatatcgc catcgctgta atcgtctatt aactgatcat
    27241 tcggaaaatg cggtaaatct ttaaaggcat cgggaatttt attagataac ccaattttct
    27301 tcatcaaaga cttactaatt ccaaaggtaa tcgttaactt gcttgcacct aatcctatcg
    27361 attcaccggt atctactggc ggcattaaag gattgttact ggccttgcca actgcatctc
    27421 catccatcat acgatcagcc ataaccgtcc atttcttaaa cattgcttta atcgcatccc
    27481 gatcttttga tttcaaatct aatgcaacga atttacatgt tttttgcgtc ggtgtggtaa
    27541 tgcctggttg cactttacca taaaattata cgcatctttt tccggatctt ctggtgtatt
    27601 gaacattgat ttgaaagacc acatgctacc aacaccactt gcgccaattg caacaccggc
    27661 accgccaata cctaacattt tcaaaaatgt acgtcttgaa aattgcgtac tattgttaac
    27721 ttgttcataa tttgtcattc agtaatcaca gccattttac ttagtggctc tcctaatgca
    27781 ttaacagcat ctgcaaatgc tttacggtct ttcttagata ctttttcaaa cgactcataa
    27841 ccgccgttgt tatctttata tttatccaat aactgattca ctttatcgaa gttcatttga
    27901 atatcatcac ttaatttttt atcttttttc tctaaaatag gtttaaataa gtcataaatt
    27961 ttttgtgcgc cttcaacgtt cgctttaaaa tcatataaat ctgtatgtga ataaatttct
    28021 tcttcacctg tgattttaga agttgcaact tcatttaata ggtcaacaga accttgtaac
    28081 attaattttg gtgtgatatc taatgtatca gctttggcat gcaattcttt tgcatctttc
    28141 aataattgtt gtgcatcttt tttagtcaca tcatcaattt tcttatcttc gtataatgct
    28201 ttttcaatct tatgatatcc tgaccattct ttttcctttt tctcttcttt catatctgca
    28261 agacgtgcat caattttagg atctaaatct ccaaatgctt ctgcaactgg ttcagagcgt
    28321 tcataataca tacgaacttt tggatataac gcttttgctt gggccatatc attattttca
    28381 atcgctttaa caaatttttc tgtacctttt aaaaattcat ttaactgttc atcagtatat
    28441 ttatcatatg cttttgttgc ttgttttaat tctgctttaa caccatcatc ttttttcgat
    28501 gtctttgaat catccttctt actatcgtcg ttcccacatg cagcaattaa taacgttgat
    28561 gctaataata gcgttgttaa ctttttcatt ttgacacctc attatagaaa ttctattatc
    28621 cccatagaca tgccctcatt cgtactatct cacactccct aaatgccaag ataatacgtg
    28681 gctcttaaaa tgatgatttt aaggcatcct cccatgaaca ttatctactt gataataatt
    28741 atcaaaatca ttatattgtt ataaaataat actgtcaaca acctttttag tttgaaaata
    28801 aataaaagag taatacttat atgtaagtca ttactctttt gtagtctgat ttatcatcct
    28861 tcattatatt ctgattttaa taaactatag atgtaactcg atgaataaat accatttagt
    28921 aattcattgt cacgtaacat accttctcta gtaaatccta gcctttcagg aatagctcga
    28981 cttttttcat tatgaactgc ggcactgatc tcaattcgat ttaagtcaat ctcatcgaag
    29041 caatacttta tcaatgcctc aactgcttgt gtcataatcc catgaccctc aaattgttta
    29101 tctaaataat accctattga agtttttctg tgcatgtgat taatttcatg caaaccgata
    29161 acaccaacta gcattccctc ataccaaata ccacactgaa atccatgacc atcagcaaat
    29221 tgcaaaagtc ctcttttaat aaatgcacgc gtatctgatg gttgctcagt tgcatctacc
    29281 caaggtaacc attccctaag tgaatttctt gaacgattga ttaaattgaa aagcgcttct
    29341 gtgtcatggg cttctaaaat ttttaatctt atttgttcat tcactttcat tccaaacata
    29401 ccatcacacc ctcatttatt tttcatataa ttctgtaaat tatcataata ataacatatt
    29461 aatattacgc cttgtatctt aaatttgttg tccttcccca acttgcattg cttgtagaat
    29521 ttcttttcga aattctctat gttggggccc cgccaacttg cacattattg taagctgact
    29581 tttcgtcagc ttctttgttg gggccccacc aataagggtt gtagtgccta ttactttgat
    29641 tgatgtcctt gatacctatc acacaactta atttaataac tactttatta accattccaa
    29701 atttatattt aaaaatacta caaaacagtg ctaaaatgct attaaaggag gcactgctca
    29761 tggcatcatt gaaaaataag cattttatga ttggactatc actaacattt atagtcgcat
    29821 tatttagctt tttagcagct aaattgccaa tcctagataa ggttggcgca ttaactatcg
    29881 cgatattaat cgctattcta tatcgtcact ttagaggata tcctgaacaa tatagctcag
    29941 gtattacgtt ttcatctaaa tatttattaa gatttgcaat tatcctgtac ggacttaaac
    30001 tcaatatatt tgatattatc ggtcaaggta gtagactatt agccatcgat gtaggtgtcg
    30061 ttatttttag tattgtaatg atgctctttg tcaacaaact gttgcatggt gacaaaaaca
    30121 tcgcattatt actaggtgta ggtacaggcg tttgtggtgc tgccgctatt gctgcagtag
    30181 ctccaatatt caaatcacgt gaaaaggata cagctattag tatcggtatc atcgcattga
    30241 ttggtacgat attttcactt atatatacag ctatctatgc tatcttttca atgacgacaa
    30301 atgtttatgg cgcttggtcc ggcgttagtc ttcatgaaat tgcacacgtt gtcttagctg
    30361 gtggctttgg tggtagtgat gcacttaaaa ttgcacttct tggtaaactt ggtagagtat
    30421 tcttactgat tccattaacc attgtactta ttttaattat gcgtttccgt tcatcagaat
    30481 catctagcaa gggtcgaata agcattcctt actttttaat tggatttgtc attatggcct
    30541 tagtgaatac atatgtaact attccgtcag cattgctaaa tattttaaat actgtatcaa
    30601 caatttgctt attaatggcc atggttgctt tagggttaaa tgtggcattt aaagatctta
    30661 aaaatcgcgc acttaaaccg cttatgacaa tcattattac ctcaatatgc ttgtcatcac
    30721 ttgcctttat cgtcgttcac tggctatata gttaaataaa aagacgctca aacacttaca
    30781 aatttagtgt gccgagcgtc ttatttttat actttaaaag tcgtattggt tgttttcttc
    30841 ttcaagcaca tgttgttgta ctttagcagt tcgcattgtg gattgtgcca acgcttttaa
    30901 acgaaataca acatcatcat tgacaatttg ttgatttgaa aaatctttct cttcaataaa
    30961 tacatacgtt tgcatcgtat gtgctttcat gtaactcaat attggtttta aatgcatttc
    31021 aggaattaaa taatgtttac tagaacctgc tgtcgctaca agtcctatta ccttgtcacg
    31081 aaacgcattg accggaagca aatcaaatac atttttcaaa gcaccaggga tggaagcttg
    31141 aaaaattgga aaaccaataa aaatcacatc agcctgcatt aacgacgtcg ttaatttata
    31201 tacatctcct gtagtatcta gataattctt tccaacacta aattctaatt cgaggtctcg
    31261 taaatccatc aattctattt ggtgtccctc attgatgact tctagttcat tttttaaatc
    31321 atccatagca attctcgttt tagaacctac tgtggaacct gacaataata caatattcat
    31381 gatgacgccc ctcctatttt gataaatgct ttttaatcgc cggtataatg tcgttgccaa
    31441 ttaactctat attcttcata acattttcaa atggcacacc gccaaaatca agctgtgcca
    31501 taaaacgttg atgaccatac aactcgtgtt gatacaatat cttttcaata atttgttgcg
    31561 ggcttccaac cattagcgct tctcgataat ctgacgaatt agcaaattgc tgtttcggat
    31621 aaccaacgcc acgaataaat gacatccctg tattcaaatg tggataaaat tctctcatag
    31681 catcctgagt tgtttcagct gtataaaaca gactcgctgt acttactggt aaagacttag
    31741 gcgaagcatc gaaacctgct tcagttgctg cttgacgata agcatctata gaacctttaa
    31801 agttcattgc tgggccacca agagttgtaa tcatcattgg cacaccttgt tttcccgctt
    31861 taatagcact tgcaggtgga ccaccaacag cacgccatat tggcaatgta ttatcgattg
    31921 gtcttgggaa tattttcata tttctaagtt ctggacgata ttttccagac caagtaatac
    31981 gctcagtttt atttaactct aaaagtaaac ctaatttttc ttcaaacaat tcatcatagt
    32041 cttttaaatc atagccaaac aagtcaaaaa tacctgttct tgatgctctg ccagctacaa
    32101 tttcggctct accatgagaa atcaaatcta atgtcgcgaa gtcttcaaat actcttacag
    32161 gatctgttgc actaataatc gttgaagaac ttgaaacttt aatatgcttt gtcgcttggg
    32221 caattgcacc taacacaacc gtatgtgcct gtgttgtaaa atgctcctga tgactttcac
    32281 caactgcaaa aacatcaata cctgcttcat ctgctaattt acttgcttca ataatttcat
    32341 taatacgttg ctcataacta actttttcac ctttcaatgg attcaataaa tgatcaccta
    32401 aggaatacaa accaaactca agaggcgtat ttttattcat ttctaatttt gccattaaac
    32461 attctccttt atatttcgtg taacttactt tcaatttgtt cacgttctgc ttctaaaaag
    32521 tcaggcaagt tcaattgact tcctaattgt tcaagaggtg tatcaatagt aaatccaggc
    32581 gcttcagtcg caaattcata cataattgaa ttatggcgat agtataatga tttaaagaaa
    32641 tagcgatcaa ttatacctga attactttgt ggttgttgtt gtaatttctt gtaaattgca
    32701 tctaagtcac tcatttgagg tgtattaact gcaatatgat ggatataacc tcgtccaggt
    32761 ctttcacgtt gtccctgttg ctcaataact acaaaatccg aatataaacc ttgtggtgct
    32821 aatgtcagca catcgaatga tttattatct cttttgcgat aacctaagac attttccaaa
    32881 aattctaccg tacgcgctgc atttctaact cttaattcga ctggccctat tcctaaaatt
    32941 tgatatgctt gaggtatttc actataagcg ttatgttgcc attgatgtgg tacttcaaaa
    33001 ctatcattaa caagcagtat gatttctaag tcgtcttcat ctttaaagac aataccttct
    33061 tgtccaagat agtacaaacg ttcagactta ataccattat tatcaaggcg tttttcaaaa
    33121 taatgaagtg ccgcttgatt tggtactaat aatgaaaatc gataaatcgt ttcagtacct
    33181 ggctgcttat gacccgcatt gggaatttca aaaaagctta aaattgttcc agctgtacct
    33241 acttcgtccc cataaaacaa atgatacatt gacggattgt cttgattaac cgacttttca
    33301 actaatcgta atccaaggac atttgtgtaa aaatccttat tacgttttgc gtcttttgta
    33361 tacatggata tgtgatgatg ccctactata ttcatactat ccctcctatt agtataaatt
    33421 ttataccaac ctacataaaa aagatagtta tatgccattt accccttttc tgcaaataaa
    33481 aatatgtttg cactccattc gtagatacac agctagtaag tataacgaca taataattgc
    33541 ataattatta atttatgttc gaccatttac ttttctgact gtttataaaa tgcttcaaaa
    33601 actaaatctg caagatttca caaaagtata ttgacaacgc tttcatacat gtctaatata
    33661 agtttatcgc acaaaatgca tataaataca tattaaggag gattttatga attttcttaa
    33721 acctgcaaag catattaagc ctttgccaga aaatcagata gatgatacct ataaacgatt
    33781 acgtctccaa gtatttcttg ggattttcat cggttacgct gggtactatt tattacgtaa
    33841 aaacttttca ttagcgatgc cggcattgca agagcaaggt tttacaaaag cggaactagg
    33901 ttttgcactt tctgctgttt ccatcgcata tggatttagt aagttcttta tgggtactgt
    33961 aagtgatcgg agcaatgctc ggatattctt agttcttgga ttagcactca ctgctatcgt
    34021 caatttgtta atgggatttg taccgttctt tacatcaggt atcggtatta tgtttgtcct
    34081 attattctta aatggatggt ttcaaggtat gggctggcca ccttcaggtc gtgttctcgt
    34141 tcactggttt agtgtaagtg aacgcggaag taagactgcc ctttggaacg ttgcgcataa
    34201 tgttggtgga ggtattatgg cacctattgc tgcttggggt attacaacaa cagcatttat
    34261 caactttggt tatttaaaag gtttcgaagg tgtattcatt taccctgcac tcttagcact
    34321 tatcattgcc gcaatttcat acgtattgat tagagacaca cctcaatctc aaggtttacc
    34381 tccaatcgaa atttataaaa atgactttgc tacaagcgat aagaaaacat tagaaacaga
    34441 attaactaca aaagaaattt tatttaaata tgtactaaac aataaatggg tatgggcaat
    34501 tgcctttgca aatatatttg tttatttcgt gcgttatggt gtacttgatt gggcgccagt
    34561 ctacttaagt gaagaaaaac atttcgactt aaaagcatca ggttgggcat acttcttata
    34621 cgaatgggct ggaattcctg gtacattatt atgtggttac atttctgata aattattcaa
    34681 aggtcgtcgt ggacctgcag gtttcttctt tatgttaggt gtcacagtat ttgtattaat
    34741 ttattggtta aatcctccag gcaatgcttg gttagacaat gtctcattaa ttgccattgg
    34801 tttcttaata tatggaccag ttatgttaat tggtttacaa gcattagatt atgtacctaa
    34861 aaaagcagct ggcacagcag ctggattaac aggattattt ggttatctgt ttggtgctgt
    34921 aatggccaac atcgtcttag gtgctgtagt tgataaattc ggatgggatg tcggttttat
    34981 tttattaaca gcaatcagtg tgtttgcaat gttgagcttt atcctcactt ggaataaagt
    35041 aggacaagaa accgttcatc attaaatgat aaaaaatatt tacattgcca cttttgtgtt
    35101 tgccctgctg ttttattcaa ttgattacac cacttaggat aaactctaaa agccatctta
    35161 cctttatgtt caaatgtagt taagatacct ttttctacca ataattccct aggaaataaa
    35221 aaataaccgc ttaattcttc atcaataact ataatcatta agtaatctgc aaacgcctct
    35281 ttcgagtatg gttgattaca attgttctca tcttttgtcc aacatgcaac aaaataacct
    35341 gctttagtcg gtgttttctt agctaaccta ctttgatatg tttcctcttt aaagctaaat
    35401 gttaatgctt cgtaatcttg attatatttt tcttcagtta aatctttaac gtctaattct
    35461 tcactaaaaa tatatttcaa cagtatttta gatttataca tatgccaatc tcactttatt
    35521 tttcataatc gtatcatata tttatttttt tcgaaaaata cacttccgac cgaaatatac
    35581 tcagccagag gtgtacgaaa tatttaacct tctattaatt cagatgtatc aactgtcaaa
    35641 cgatcacgta ataaatagac gattaacatt gctgctaaag cacaaagtga ctcagcaatt
    35701 aataatgacc aaatgacacc tgttagtcca aacaaagcat tcataataaa taatacagga
    35761 atgataatcg caccttgtaa aatggccata attgtagcac cgcgaccttg accagttgct
    35821 tgaagcatac cagtaaacaa gaaacctata ccatttaata acaatgatgt cattgttact
    35881 ttcaaaataa atgtcgccat ctcaacaatg gcttgatcag tagtaaatag tccgaccata
    35941 tgatgtccaa ttgtaaatac agcaatcata catacaacaa agataacgcc gatagacatg
    36001 ataactgttt tgataacgtc tttcatacgg cctttatttg ccataaagtt atatgcaatt
    36061 agtggtacaa caccttcaca taatcccatg ataataagtt ctggaaattg cacaagtcta
    36121 aatgagatac cataacttgc aatcgcgaag tttccataat gtgctaaaaa taaatttaaa
    36181 actaatcctg tgaatcccat taagatactc attaaaaatg caggaatacc gattttaaag
    36241 atttcagaaa gcatttcttt attaggtttc gcaagtttaa tattaactga cacaacgtca
    36301 ctatttttca taaaataaac gataaagaat agagcagcag caacattact gattgcagta
    36361 cccaaagctg caccaacaac gtttaaatca aaaccaaaaa ttaaaattgg atctaaaata
    36421 atatttaagc ctacactagc taacatacca atcatagaaa tcattggtgc cccaattgca
    36481 cgtgcaaatt gttctaatat gaagaacaaa attacaaaag gtgcacttaa aaacattact
    36541 ttcaaataat tacttgttaa agctaacgtt tcacctctcg cccctaaaat tgctgcgatt
    36601 tgatcactga atggtaaagt aactaaaatc acgataagtc ctagtgcaat accaccataa
    36661 atagagaaac tacttacaaa tttactctta ctatagtctt tcgcacctaa taaacgtgaa
    36721 atataagttc ctgcaccaac gccaaataaa ttacccaacc ccattaagat agcaaaaact
    36781 ggcagtgtta gagagatagc agaaatcatg tggctatctt ctaaaaatcc tataacgtaa
    36841 atatttaata tgccataaat aacgcttaat aaagtcccta tcatcattgg caatgagaaa
    36901 tgcatcatcg ctttaaatac tggcgatttc tcaaaataat ataattgttc gtctttcatt
    36961 gttcaatact ccttgtcttt ttcaataatt agcttactaa caattagata tctaactata
    37021 atattaaaga taaagtgact gatttctacc agtcacgctt accatttatt gtaaactaga
    37081 taacatttta gttaagtttg ctttcatttg ttcattttct tcttcagata actgcgatac
    37141 gagtgtttgc tccatttcat caaatatcga agtgaatgct tctacaagtt taatcccaga
    37201 ggtagtcagt cctatattct ttcttctcgt atcttgtgca tcgacatagc gatagatcag
    37261 ctttttacgt tcaaggttcc ttaataaatt actgacagtt ggacctgttc gttgtaatgc
    37321 tttagcaata tcattttgtg tcagtccatc ttgttgatgt gcataaagat aacctaacgt
    37381 atgaccttgc tcatttgtaa tatcaaattg ctctaacttt tgatcagcct tttgtttcat
    37441 ctcatgacta atcattctaa ataaatacga ataagtgaat tccatttatt gcaccaccat
    37501 ttcgttagat gtctaactaa atatactgcg acatttttaa attgtcaaca tgaatatcta
    37561 atttatttta aaaattccta taaaatcttg cgccataaac attttgatat gcaatttttc
    37621 ggattaaaat atgaaagaga ttgcatatct acgttttcga gaaatcgctt acaattacaa
    37681 ataggaggat attacgttgt taagttatag acaaatcgaa attttgtatc ttttaattca
    37741 taagcaaacg tttattccaa tcaataccat tgctaatcaa cttggtgtat cacctcgtac
    37801 cattcaatat gatatagcat atattgagca atatgctgaa acttatcatt accaagtcag
    37861 tcgtaataaa gctgcaggca tcaaagtaac aacagctcac gcgacactat taaatgaatt
    37921 agagcacaat ctaacgaatc aaattcattt ttcaaaagat gagcgactca cacatatcgc
    37981 tttaaagtta ttcgaaacaa ctgaccctgt ttcaacaaag caacttgcgc aagatgttaa
    38041 tgtttcgcgt cggacaattg cagatgatat taaaatgatt caagcccaac tagatcaata
    38101 ccatttaaag ttaaattatg tccacaataa aggattcaat attattggtg aggaagatta
    38161 ttatcgtaaa gcgtatgcac actttataca tcaatatatg aaacaagctg caccttttat
    38221 agaggctgat atctttaatt cagaatcaat cgcattggtt cgccgtgcca ttattaagac
    38281 attaaatagt gaaaattatc atttagttca gtcggctatc gatggcttaa tctatcatat
    38341 actcattgcc attcagcgtc taaatgaaaa tttttcgttc gatataccta tcaatgaaat
    38401 taataaatgg cgacatacta atcagtatgc tattgcttca aaaatgatag aaaacttaga
    38461 acgcagttgt aatgtcacat ttccagagtc tgaaattatc tttatcacgt tacatttact
    38521 aggctctaaa atgaccgaac atacagcatc ttcaattacc tttgaatatc atgatttatc
    38581 gcaaaatata catgaattga tcacttgtgt tagccaagaa ttaggcattg atatgtcaaa
    38641 agacaacaag ttgcatacca gtctgatcac acatatcaaa ccagctatac atcgtattaa
    38701 atacgatatg ctacaaccta atcctttgag gcaagaagtt atgcgtcgct atcctcaaat
    38761 catagaagcc gttagcaagc atattagccc aattgaacag gatgctgcta ttcgcttcaa
    38821 cgaagatgaa ttaacataca ttacaattca tttcgcatca agtatagagc gtgttgcaac
    38881 acataagcaa tcaatgatta aggttgtctt actatgtggt tctggtatag gcacgtcgca
    38941 acttttaaaa tcaaaactaa atcatctgta tcctgagttt cacatttggg atgcctattc
    39001 catttatcaa ttggaagaaa gtcgattatt gcaagataac attgattatg tcatttcaac
    39061 agtaccttgt gaaatatcag ctgtaccagt tattcatgtc gatccattta tcaatcaaca
    39121 atctcgtcaa aaattgaatc aaattatcaa tgactcaaga gaacaacgag tcatgaaaat
    39181 ggcaactgat ggcaagtcac tcgcagattt attgcctgaa catcgcatca ttataaataa
    39241 acaaccatta tcaattgaat ccgcaattgc agtggctgtg caacctttaa tcaatgatgg
    39301 cattgtctat tcaaattata cagctgcaat tttaaaacaa tttgaacaat tcgggtcata
    39361 tatggtcatt agtccacata ttgcacttat tcacgctggt actgattatg tacagaatgg
    39421 tgtaggtttc gcactaacat atttcactga agggattatc tttggtagta aagctaacga
    39481 tcccgttcac cttgtaatta cattagcaac ggaccacccc aatgcacatt taaaggcatt
    39541 gggacagtta agcgaatgct taagcagcga cttatatcga caagatttct tagatgggaa
    39601 tatttttaaa attaaacaac acattgcttt aactatgaca aaggaggctt aataacgtgt
    39661 cattagacat tttgtcaaca acacgcatca ttgtaaaaga acaagtaaat gattggactg
    39721 aagctataac tatagcttct cagccattac tacaagaaca aattattgaa caaggctatg
    39781 ttcaagcaat gattgatagc gttaatgaac ttggacctta tatcgttatc gcacctgaaa
    39841 ttgcaattgc acatgcaaga ccgaacaatg acgtacatca agttggttta agtctattaa
    39901 agttgaacca acatgtggca ttctgtgatg aaaatcacta cgcatcactc atttttgtat
    39961 tgagtgccat cgacaatcat tcacacttat ctgtattaca aaatttagca accatactgg
    40021 gcgataacca aacagtccag caactattaa ctgcaacaaa cgcacaagac attaaaaaca
    40081 ttttaaagga gcatgattaa tatgaagatt ttagtagtat gtggccacgg tttaggaagt
    40141 agttttatgg tagaaatgaa cgcacaagaa gcacttaggc aacttaatgc accatctgat
    40201 atcgaagttg aacatagtga cattatgaca gcaagtccag agatggctga cttgtttatt
    40261 tgtggtagag atttagctga aaatgccgaa cgtctagggg atgtcttagt tcttgataat
    40321 attttagata aagctgaatt acaacaaaag ctctcagaaa aattacaaca acttaacatg
    40381 atttaaagga ggtacgacct atgcaagcaa tccttaattt tatagtcgat attttaagtc
    40441 aaccagccat tcttgttgca ctgattgcct ttataggttt aatcgttcag aaaaaacctg
    40501 ccgcaacgat cacttcagga accattaaaa cgatattagg cttcttaatt ttaagtgcag
    40561 gtgctgatgt cgtcgttcga tctcttgagc cattcggcaa aatatttcaa cacgcattcg
    40621 gtgtgcaagg tatcgtacct aacaacgaag ctatcgtctc actagcctta aaagattttg
    40681 gaacaacagc tgcactcatc atggtctgtg gcatgattgt taatatttta attgcccgct
    40741 tcactaattt aaaatatatc tttttaacag gtcatcatac attttacatg gctgcgtttt
    40801 tagcaatcat tttaacagtc agtcatatta aaggctggct aacgattgtt atcggcgcac
    40861 tcgtattagg attaatcatg gcagtcttac ctgcattact ccaacctacg atgcgaaaaa
    40921 ttacagggaa tgaccaagta gcgttaggtc attttggctc aatcagttac tttgccgcag
    40981 gtgctgtagg tcaattattc aaaggtaagt ctaaatcaac ggaagagatt aaatttccaa
    41041 aaggcttaag tttcttacga gaaagtacaa ttagtatctc gattacgatg gcattacttt
    41101 acttcatcgc atgcttattt gcgggcgtta gttatgtaca cgaatctatt agtgatggtc
    41161 aaaactttat tgtcttttca ttaattcaag gtgtgacatt tgctgctggt gtatttatta
    41221 ttttaaccgg cgttcgttta atcttagctg aaatcgtccc agcatttaaa ggaatttctg
    41281 aaaagcttgt accaaattct aaacctgcat tagactgccc tattgtgttc ccttatgcac
    41341 aaaatgcagt attaattgga ttctttgtca gctttattac aggtgtcatc ggtatgttta
    41401 tcttattctt atttggtggc gtcgtcattt tacctggcgt agttgcacac ttcttcttag
    41461 gtgcaacggc tgctgtattc ggtaatgcaa gaggcggtat taaaggtgct gttgctggcg
    41521 ccgctctaaa tggtatccta atcacgtttt taccattatt attcttgcca tttttaggcg
    41581 aattaggtgg tgctgcaaca acattctcag atacagactt tttagctgtc ggtatcgtgt
    41641 tcggtaacgc agtaaaatat atgggattat ttggtgcgat tctatttatt attatcgtag
    41701 gtgcgacagc aattttatta aaaggtcgtc aaaagccaca gcaatagtgt taacgtaaaa
    41761 atataaaaca ccgtcacata ttgagtgaat gcccctttta tcaagaggaa agccacttac
    41821 ttatggacgg tgtttttgta ttatattaaa tgaaatttaa ccatactatc aattgctgct
    41881 aaaattgctt cttcttgtgt cgcaatcggt tcccaaccaa gtaatgtttt tgcacgttcg
    41941 ttacttacat tgcggttcat atctaataaa agtttacctt ctttagcttg atgattaaat
    42001 ttagcaccta gactcaaaat aaagtctggc aattttttag tagcaacttt ttgagctatt
    42061 tcaggtcgtt tttctttaat taattttgca atttccaata aattaatttg tccatcagcc
    42121 gtagcaataa atcgcttgcc attagcctgt tcatttgtca ttgccaaaat gtgtagttct
    42181 gctacatctc tcacatcaac aacatttaat ggaatttgag gtacacgttt cattgaacca
    42241 ttcaataaat tttctaataa atgaaagctt cctgaaacat gtgcatctaa tgatggtcca
    42301 aaaattgcaa ctggattgat tgtggcaaat tctactgtcg tattttcatt gtcaacaaaa
    42361 tcccacgctg ccttttcagc taacaatttt gatttttcat atactgataa gcctggttca
    42421 tcttgatttg tccaatgact ttcatttgtg attgaatttt tatctttatt actaaaacca
    42481 actgcaccaa agtttgcagt catcaccaca cgcttcacgc cagcatgttc tgcagctctt
    42541 aaaatacgtt gtatgccttc aatggcaggc ttcgccatca cttctgcatc gtctgtttta
    42601 ccgaaaaaca ctggagatgc cacactcaac acatatttac aatctttcat tgcttcatcc
    42661 caatgttcat cttgtgataa atccgcttcg acaaacatta atcgctctgt ggaaatacca
    42721 ttgtcttgca ttgttttaat tactttatca gctttactta aatcacgtat cgtcgttttg
    42781 acctcataac cttgttctaa taatcgtgaa atgattcgca tgtcaacaaa ccctgtacca
    42841 cccgtcacta atactttatt attcatcaca taatctcctc tttgtgtcta ttgattactc
    42901 attatagcag tcattatatt ttgacgtata taaatagact ttggtagcta gtatttaatc
    42961 ttactggtat aaatgaaaat gaatttattc ctttaaaaac tatcattgac cattttcaaa
    43021 tacccaatca ctaataaaca caaacgctat aaatatgatg tctttgctaa ttaactaaat
    43081 tcattttcaa tcgtatgttc aaattacata actacatagt caccatttta aaagcaaata
    43141 cacatattca acgagggtgc gacattaacg cctaaacaaa attaacttaa aatcatctca
    43201 accaatgatt caaccgtaac attaccaaaa taataaacga tgtctaattg ctttaactga
    43261 tgcgttaaat cttctcttgt catttttgtt ccttgtagcg cttgttccac atcttttata
    43321 tcaccttgtc caaagaaatc accaaaaatt ctacaatctg caatacgatt ttgttcaaca
    43381 gaaatggtaa tatctacagt acctgatgat aatctttcac ttcgattata ttcatatttg
    43441 ggtgacttgc cataattcca atcccaattt ttatactttt tatcagctaa ttcatcaatc
    43501 gcttcccaat ctgcttcgga taattcatac ctttttgcct ctttaatgtc atcgattccc
    43561 aaaatctgcg tcaccattaa atttttaaat tcttcaatgg ttataccacg atacttttca
    43621 tccaatgctt ctctaagatg accaacacgt gcgcgcacag atttaatccc tttcgatgca
    43681 atctttttgc gattaggctt taacacttta tccattgctt cataattaac atcaagtaat
    43741 agcgaataac cgccataaat acgattgttc attaatgtca ttgcggcgcc tgacactttt
    43801 ttgccgttca atgttaaatc atttctaccg ctttgtatca catctcttgc acctaatgta
    43861 tgcaatgcct ttatagctgg ttgataaaat cgttgaaaat caccataaat tgaagtgtct
    43921 tgttctaaaa tacaacacat attaacggca cctttatcaa catacacagc gccaccacca
    43981 gtatctcggc gaactacttg aatactgtgt ttatctatat aatcttgatt cacttctata
    44041 gcagtatttt gaaaacgtcc aatttccact tttggatcac aataataagg gaataaaata
    44101 tcgtcatcta aaaagatatt ctgattaaca taaacttgca tggcgagtgc aatcgcacca
    44161 tcagtaatat attctccatt cctaatcggt tctattaagt acatcttctg tctctccaat
    44221 gttgttattt ttcgttgtgc tatcgttccg gagtgctgtt gtaatcaatg ttgagatatc
    44281 atcagtcaaa tgtatggtac gttcttgaat tgaattcgga atgcgatatg cctttttatt
    44341 catcgtcata taaagtgcat tttcattttt acgtgtcata cgctgaaaag gatgtttcac
    44401 aaattgtggt gtagtaaaac caattccaat ttccaaatac aacactttat catcttgatg
    44461 ttgctctaga aaagcattat aacgttgcag ttgcgcatga aattcagcat cttcaaccat
    44521 gccaacttcc gctttacgtt tattcacttc cattggagca tcacattttg gacatcttgg
    44581 aatcatctcc caaggtataa gcatatcttg ttgcgcaaca accattttac gaattaaatc
    44641 atcattgcga tacgtttgag catgacaatg ctgactacac tgttgcagta tatactcgcc
    44701 ttgtatatga aatacatgcg tcatatcata atcagctaca tcgaaagcat tatctgcatt
    44761 cgtagttata atgtggtact gtttaccttc caccaaggat tttaaagcga ggtaagactg
    44821 acctacaggt tgatctaaat agtttaatgt aataaaacga ctctcaaacg cccaatactc
    44881 ttgccaactg ccataaggat gtaaactcgc ttgcaacata tcaaagaagc gatatttttc
    44941 aataaaatcc gggaaatttt ccgtaaaacg ctctcctaca tatgtaaatc cgtcagatgc
    45001 agacatgcct gcaccaattc caatcactat cgcatctgct tcatcaatcg cagtacgcaa
    45061 tacttcagcc tgctttgtct tgtcatccat taacagagac attgcattcc atttactact
    45121 ctgcatcacg gttcaatgcc tccttataca gttgtaaatc cttatctgta aatacattga
    45181 acacgacttt caatgttgaa tttgtttctt tgagatagct ttctactgtt cgaacagcaa
    45241 tttctgctgc ttcatcttga ggaaaagcaa atacacctgt agatatacag caaaaagcga
    45301 catgatttaa actatgttga tcagccaatt taagacagct aagataacat ttagctaaca
    45361 agtcatgatt catctttgaa acaggcaatc gacgtatttg cggaccaacc gtatgaatta
    45421 tatactttgc tggcaaatta tatccacgtg ttattttggc tttacctaca ccttcattgc
    45481 gcccttgttg tcgaatgatc tctgcacaat caagtcgaac ttgaacaccc gcttttgtat
    45541 gaataatatt atcaatgcag tcatgattag cttgcataca tcctagaaaa cgactatttg
    45601 cagcattaac aatagcatcg atttttaacg tcgtgatatc accttgccaa acaaagatat
    45661 tgtcaccttt tatcggcttc aaatccttca aatccgtaac atgttgacga ttcaaatcac
    45721 ttaacatagc atcttgtaca gctaaatatt catcacttac aggtaatgcc ggtctgacat
    45781 ttgctaagcc tcgatataat tcccacaaat cttcaaatga agatggcatt accaatacgt
    45841 cattgtcatt tctctctcga cgcatatcat tgattaaata ttcaagtctc gctttatttg
    45901 attttaacgt ttccatttaa gcctccggta atgctaggaa tgcttcttta tcgacatcat
    45961 ccaatttgaa caaccaattt tcttctggtt tttcagagtt taaaattgtc ggttcttctt
    46021 ccgcttttgt atttcgctca ataatcgttc ctgacaatgg cgtttgcaca tcaatgaccg
    46081 ttttcgatgc ttcgatactc acaatttcat catccacttt aacttcatct ggacttacga
    46141 attcaacata acctactgtc ccaatatcat cttgcaattc aggtgtcata ctaaacacat
    46201 acaaatctcc tactttttct acccataaat aattggctaa ctttttcatc ttatcttcat
    46261 cctttctctt ctatttaaaa tgctacaaat agatttccgc gagtaatttt aatgttttac
    46321 aacgctgttc aatgcctgga ataagcggtg ctactaacac ctcatcaact tcaaacgtag
    46381 caatgaaatc atctaattgt gctctaacct gttcttgtgt acctgcaatg atgcgtgctt
    46441 gatgtgcttg aatcatctct ttgtctcgat cattaagctt atacttttgc gctgtgtcta
    46501 ccgatggaaa atgttcaaat tctgcaaatt gtaatttacc taataaccaa acatctaagg
    46561 catgttgtaa tgctgctact tccgcttcgt tatcagctac aatgacaaat acagatgcca
    46621 tcacctttgc gtccatttta atcgttgatg cttggaaatg ttttttgtaa atatcaatgt
    46681 tatccttcgc tgcatgtatc gcatttatat ccggtagcaa aaatgttcca acagaaagtc
    46741 ctatacctag ttcagcagct attttggcag atgttgcgct actacttaat aaccacattt
    46801 ctggaaaatg atcaatgtgt ggttggacac ctaacgtatg cgcacttggt ttatccttta
    46861 ttgtaagata atcacgtaat aacgaaatcg attcatcgta actatcatat gtaggattta
    46921 ttccatctaa agcttgcttt accatagtag tacctggatt attaccaata cctaaatcaa
    46981 tacgatttgg atataacgct gccatcattc taaaatgctc agcaatttta taagggcgat
    47041 agtgcggcaa catcacacca ccagaaccaa ctcgtatgtg atttgtctgc gccaatgtat
    47101 gcatcatcaa aagttctgga ctactacacg caaacgctgg tacattatga tgttccgtaa
    47161 accaaattcg cttaaagcca agtcgatctg ctaattttgc aagtgtcact gaatcctgca
    47221 acgccttttg tgcatcctta ccttcatcta ttaaggcata gtctaatacg cttaatttaa
    47281 ccaatccgtc atctccaaac ttatcctgtc atgtcaaaca gacataacat tttagcgttc
    47341 ttaataccat tccctcttca tatacccacg tatatgataa cgtttttcaa ttaactttta
    47401 tatcttttcg cttgtatttt tctttttcat attcatattt tcagacctac aaatgacatt
    47461 cattagttca attttaattt taaaaatgct ttccatgtac caattctatt ctatcaagac
    47521 tgttcatcat tgattattca ttctttccat ggtactttga aaacaatatt gaatagaaaa
    47581 ggaatcatag tatgtaccga tacaaaccac ttttacaatc aatccactta cctaatggca
    47641 tcaaaatttc gaatcgcttt gtattatcac caatgaccgt aaacgcatca acaaaagaag
    47701 gctatattac aaaagcagac ttggcttatg ctgcacgtcg ctctaattca gctggtatgc
    47761 aagtcaccgg cgctgcttac attgagccat atggtcaatt gtttgaatac ggctttaata
    47821 ttgatcatga tgcttgtatc cctggattaa ctaacatggc atctacgatg aaacaacatg
    47881 gtagcctcgc tattattcag ctagcgcatg ctggtcgttt ttcaaatcaa gcaattttaa
    47941 attttggaaa ggtgtatgga ccaagcccta tgacgttaca ttcaccgatt gaacacgatg
    48001 ttatcgccat gtctcatgag aaaatcaata gcatcattca gcagtatcgc gatgctacat
    48061 tacgcgcgat taaagcaggt tttgatggtg tggaaatttc gattgcacaa cgtttgctga
    48121 ttcaaacatt tttctcaact ttttcgaata aacgtacaga ccattacggt gccgactcat
    48181 taaaaaatcg tgcaagatta tgtttagaag ttatgtgtgc ggtacaagaa gtgattgata
    48241 aagaagcacc tgacaatttt attttaggat ttcgagcaac gccagaagaa accagaggta
    48301 gtgatttagg atataccatt gacgagttca atcaattaat cgattgggtc atggacgttt
    48361 caaacatcca atatttagca atcgctagct gggggcgaca tatttatcaa aatacgtctc
    48421 gtacaccagg tgaacatttc ggtcgaccag ttaaccaaat tgtctatgaa catttagctg
    48481 gtcgcatccc tttaattgca agtggcggga ttaattctcc agagtctgct ttagatgctt
    48541 tacaacatgc agatatggtc ggcatgtctt cgccttttgt cacagaacct gactttgtgc
    48601 ataaacttgc tgaacagcga cctcatgata tcaatcttga attttctatg gcagatttag
    48661 aagatttagc cattccacat gctgctttta aagatattgt taaaatgatg gattatgggg
    48721 aaggtctaaa gaagcatacc cgtgacgctt tacgtcaatt ggaacagaat tatcatgact
    48781 catcgtctta agactatttt tcatggaaat ttaagacagt gttataatga catcaatatc
    48841 aaaaataagg agcctgtata gtcgtgactg aaattaaaga taaagtaatt actaaagatg
    48901 cgtttgaatt accctacaca attattaaag cgaagcatca accaacaaaa ggtgccattg
    48961 tctacatcca cggtggtgga ttaatgttcg gtaaagctaa cgatttatcc ccacagtata
    49021 tcgatatcct tacggaacat tacgatttaa ttcaactaag ttatcgttta ttaccagaag
    49081 taagtctcga ttgcattatt gaggatgtct acgcatcatt cgatgcgatt caatcacaat
    49141 attctaattg ccctattttt acctttggca gatcatcagg tgcatattta agtctattaa
    49201 ttgcacgaga cagagatatt gatggtgtta ttgattttta tggttatagt cgcatcaaca
    49261 ctgaaccttt taaaacaact aatagttatt acgcaaaaat agctcaaagc gtaaacgaaa
    49321 caatgattgc acaactcact tcaccaacac cggttgttca agatcagatc gcacaacgtt
    49381 ttttaatcta cgtttatgca cgtggcacag gtaaatggat aaatatgatt aacattgctg
    49441 attatactga ttcaaaatat aatattgcac cagatgaact taaaacactt ccacctgttt
    49501 tcattgcgca ttgtaatggc gattatgatg ttcccgttga ggaaagcgaa catatcatga
    49561 atcatgttcc acattcaaca tctgaacgcg tgaataaaaa cgagcatgat tttgatcgta
    49621 gacctaatga cgaagcaatc actatttatc gtaaagttgt tgatttctta aacgctataa
    49681 caatggtgta atatatcatt attttaaaaa actcaattta tcatcatgat aaacagttat
    49741 tcgatcaacc atattattca tacaatttat taagctcata aaaagatacg cctgtccctt
    49801 tagttttcgc taatgagaca ggcgctttat ttcactttac tattttgaaa ttcaacactg
    49861 tgatgttact tatatctgct gtgccatgtt agtagcacga aatacatagc atattaaatt
    49921 cagaagatga atttaacttg ctttcaattg tgtaccttta ccttcagttg cttcaacacg
    49981 caacaagtca tttataatac ctgtatagaa gttggcgagt tctgcacctt tacgcttgaa
    50041 atctaggaaa tctacaccga taaaatcgac atgatcccat ccttgtatga ttggtttaac
    50101 ttgccagata cctcttcgtg tggcaggttc atcattcgta acattaataa atggttgatt
    50161 ggatggatgt aatgacgaaa tcactggtac gacaccatca tttttacgcc attcttctct
    50221 tgcatcatga ccaataattc tactcgttgt atccattaag aaaaatgtac ctaaatcagg
    50281 attttcataa cctaatggac cagtatgaga tgatacacct gtataagtcg tgtacgtaat
    50341 atttggattc atacttgtca tgttgttcaa ttttgcagag ccatctaacg ttaaatcata
    50401 ggcagcattg tcgtctgatg tccaaatttt gcttttacta acgcgtttta tatagtcaat
    50461 ataactctca tttggtaatt gtttaaagcc ccattgcgtt aatcctaaat cgatattcga
    50521 atacttgtta cccataaatc gatttaaagc gaacatgatt tttctaacag cttctgtatt
    50581 tccaaactta tcagctgctt gtgaaccatt atgtggtgtt gctaatgttg tgattgatgc
    50641 aaccatattg ttatgaccac cagtgaataa tggcgatatt tctccaccat gcgctttatg
    50701 ataggcaatt tcttctttgt taccatttct taaaaactct tccattaaac gaattgtttg
    50761 accacccata ctatgcccta caagatgtac ctttttacca ggttcccaat taggcatgat
    50821 tcctttatat gttttaccat agcgctcatg tccgtattta gctgcatgtg ctgcaccata
    50881 atctacgcga ccacctttga tgtaataata aagttctaca gcacgatcat agttgctacc
    50941 aaatgcacta acacttgctt gatgtacatt atagccttgc tttctcaatt cttcgataac
    51001 tttaaattta tttccacccc aataatttgg atataaagca ggtgcattat cgcctactaa
    51061 tcctaaaaat ccatgtacaa aaacaactgg atatttgtta agtggttgta cttgattcgc
    51121 tttaagtggt cgaatctttt tagcatctgc tgctgcattt gtagtcgaac gtgttttagg
    51181 cgcttgaagt gcatctttga ttagttgttt attatcagta tttttatctg tcggtttctc
    51241 tgaggaattt tgaccatcga tatgtttact taaatcgcca acttgtggtt tctgttcact
    51301 ttggcgaaca gtatcatctt gcgttgcatc tatgatttga tgcgcgggtt gatgtgtatc
    51361 ttgttgattt ggatgtttgt ccgtggttgc atcttgtgct tttgttgatt taggtgcagt
    51421 tttatcatta gatgggggtg ttgttgaagt tggtgctgct ttatcatgat ggggtaatac
    51481 cttttgtgcc ggaatggatg gttgtgattt atcaacatca ttcgattgtg acgttgtcga
    51541 ttgtgatcca ttacgttcag catgatgggt attttgaacc tgatcatttt ggcgttcagt
    51601 tttgttaatc gcgttatttt ggtgtgactg tttagaacct tgtcgttcag aatcttgctt
    51661 tacttgatta tcattttgcg ttctgattgt tttactattt tgtgttgatt gatgcttcga
    51721 tgattgaagc gtatctttac cttctgtcac tgttccactt ttaccattct ctttatgcat
    51781 atcgtctaat tgctttcctg actgcatttg atgtgacgtt atcgtattcc cttgttctcc
    51841 cggttgattt agtgtttctt tttgtgccgc tgcattagac gttggtgttt tttccgaggc
    51901 ttgcgcttca tgtgatgaca caataaacat tgtagccgct aacactgaca ccacgcctat
    51961 tgaatacttt ctaatactat actttctttc ttcttgtcct cttaacatat aatcacctca
    52021 aaagtatttt tttgaccccc tcttgagaat gaaaaacaac tgcaccttca atataacatg
    52081 acatatatta ttttttgatt aagactacaa aattaataaa aatttaataa tacgatatta
    52141 aatcgatact
//
LOCUS       DAHHOJ010000004        44863 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.004, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000004 DAHHOJ010000000
VERSION     DAHHOJ010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 44863)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44863)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44863
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.004"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            199..31839
                     /gene="ebh"
                     /locus_tag="NBH32_000183"
                     /pseudo
     CDS             199..31839
                     /gene="ebh"
                     /locus_tag="NBH32_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109391.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hyperosmolarity resistance protein Ebh"
     gene            complement(31898..32299)
                     /locus_tag="NBH32_000184"
     CDS             complement(31898..32299)
                     /locus_tag="NBH32_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HCD1513824.1"
                     /translation="MAKINFDAATKGNPGISTCAVVIKEDDQHYTYTHELGEMDNHTA
                     EWAACIYALKHARELNVSNALLYTDSKLIADSVNAGYVKNAKFKPYFDQLEIFEQDFD
                     LLFVKWIPREQNKEANQNAQQALYKLIKKNK"
     gene            complement(32679..33383)
                     /locus_tag="NBH32_000185"
     CDS             complement(32679..33383)
                     /locus_tag="NBH32_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HCD1513825.1"
                     /translation="MYNEILGLVTFIATFVLMVLMYRFFGKQGLIAWVAIGTIIANIQ
                     VIKTVEIFGISATLGNVMFASIYLATDILNDIYGRRVAKRAVWLGFSSTLIMIIVMQL
                     SLHFIPAPEDMAQKALHAIFDVVPRIALGSIVAYIIGQHIDVFIFSLIKKVFSSDKTF
                     FIRAYGSTFLSSIIDTALFVAIAFIGSLPGTVVFEIFITTYVLKLASTVFNVPFGYIA
                     KSFYRKGKIDKLDQGY"
     gene            33628..33822
                     /locus_tag="NBH32_000186"
     CDS             33628..33822
                     /locus_tag="NBH32_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002439757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HCD1513826.1"
                     /translation="MSRILTFSEQNALAKIDDLMNTYCNQCPIKTRLRKLEGKTKSHH
                     FCINECSIGKEIKQLGNELQ"
     gene            33834..34085
                     /locus_tag="NBH32_000187"
     CDS             33834..34085
                     /locus_tag="NBH32_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HCD1513827.1"
                     /translation="MQIISISETPNHNTMKITLSESREGMTSDTYTKVDDSQPAFIND
                     ILKVEGVKSIFHVMDFISVDKENDANWETVLPKVEAVFE"
     gene            34123..35247
                     /locus_tag="NBH32_000188"
     CDS             34123..35247
                     /locus_tag="NBH32_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HCD1513828.1"
                     /translation="MEILRIEPTPSPNTMKVVLSYTREDKLSNTYKKVEENQPRFINQ
                     LLSIDGITSIFHVMNFLAVDKAPKADWEVILPDIKAAFSGESQVLESGKDPQIDKHFG
                     EIKAELLTFKGIPYQIKLTSADQELREQLPQTYVDHMTQAQTEHDNIVFMRKWLDLGN
                     RYGNIEEVMDGVLEEVLATYPESQLPVLVKHALEENHAANNYHFYRHVSLDEYHATDN
                     WKTRLRMLNHFPKPTFEDIPLLDLALSDEKVPVRRQAIVLLGMIESKEILPYLYKGLR
                     DKSPAVRRTAGDCISDLGYPEALPEMVLLLDDSQKIVRWRAAMFIFDEGNAEQLPALK
                     AHINDNAFEVKLQIEMAISRIENGDEALGSVWKQMANRTI"
     gene            35263..35700
                     /locus_tag="NBH32_000189"
     CDS             35263..35700
                     /locus_tag="NBH32_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD1513829.1"
                     /translation="MNAYDAYMKEIAQQMRGELTQNGFTSLETSEAVLEYMNQVNADD
                     TTFVVINSTCGCAAGLARPAAVAVATQNEHRPTNTVTVFAGQDKEATATMREFIQQVP
                     SSPSYALFKGQDLVYFMPREFIEGRDINDIAMDLKDAFDENCK"
     gene            36124..37080
                     /locus_tag="NBH32_000190"
     CDS             36124..37080
                     /locus_tag="NBH32_000190"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1513830.1"
                     /translation="MLNSFDAAYHSLCEEVLEIGNTRNDRTNTGTISKFGHQLRFDLS
                     KGFPLLTTKKVSFKLVATELLWFIKGDTNIQYLLKYNNNIWNEWAFENYIKSDEYKGP
                     DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLGNVYGKQWRDWVDKD
                     GNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTMFQFYVQDGKLSCQL
                     YQRSADIFLGVPYNIASYALLTHLIAKECGLEVGEFVHTFGDAHIYSNHIDAIQTQLA
                     RESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            37280..37759
                     /locus_tag="NBH32_000191"
     CDS             37280..37759
                     /locus_tag="NBH32_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1513831.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVTSSVEGKLDEKNTIPHTFLHLIRKK"
     gene            37774..38613
                     /gene="fakB2"
                     /locus_tag="NBH32_000192"
     CDS             37774..38613
                     /gene="fakB2"
                     /locus_tag="NBH32_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCD1513832.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGVSYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVTHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            38699..39232
                     /gene="msrA"
                     /locus_tag="NBH32_000193"
     CDS             38699..39232
                     /gene="msrA"
                     /locus_tag="NBH32_000193"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1513833.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            39225..39653
                     /gene="msrB"
                     /locus_tag="NBH32_000194"
     CDS             39225..39653
                     /gene="msrB"
                     /locus_tag="NBH32_000194"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD1513834.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            39665..40165
                     /locus_tag="NBH32_000195"
     CDS             39665..40165
                     /locus_tag="NBH32_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1513835.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEFINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            40165..40386
                     /locus_tag="NBH32_000196"
     CDS             40165..40386
                     /locus_tag="NBH32_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD1513836.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            40575..42065
                     /locus_tag="NBH32_000197"
     CDS             40575..42065
                     /locus_tag="NBH32_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCD1513837.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQQTNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKDKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDSLKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENQV
                     KAFQQTNKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
     gene            42462..42971
                     /locus_tag="NBH32_000198"
     CDS             42462..42971
                     /locus_tag="NBH32_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1513838.1"
                     /translation="MIRLGKMSDLDQILNLVEEAKELMKEHDNEQWDDQYPLLEHFEE
                     DIAKDYLYVLEENNKIYGFIVVDQDQAEWYDDIDWPVNREGAFVIHRLTGSKEYKGAA
                     TELFNYVIDVVKARGAEVILTDTFALNKPAQGLFAKFGFHKVGEQLMEYPPYDKGEPF
                     YAYYKNLKE"
     gene            42983..44053
                     /locus_tag="NBH32_000199"
     CDS             42983..44053
                     /locus_tag="NBH32_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1513839.1"
                     /translation="MKKIAFTGGGTVGHVSVNLSLIPTALSQGYEALYIGSKNGIERE
                     MIESQLPEIKYYPISSGKLRRYISLENAKDVFKVLKGILDARKVLKKEKPDLLFSKGG
                     FVSVPVVIAAKSLNIPTIIHESDLTPGLANKIALKFAKKIYTTFEETLNYLPKEKADF
                     IGATIREDLKNGNAHNGYQLTGFNENKKVLLVMGGSLGSKKLNSIIRENLDALLQQYQ
                     VIHLTGKGLKDAQVKKSGYIQYEFVKEELTDLLAITDTVISRAGSNAIYEFLTLRIPM
                     LLVPLGLDQSRGDQIDNANHFADKGYAKAIDEEQLTAQILLQELNEMEQERTRIINNM
                     KSYEQSYTKEALFDKMIKDALN"
     gene            44070..44684
                     /locus_tag="NBH32_000200"
     CDS             44070..44684
                     /locus_tag="NBH32_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1513840.1"
                     /translation="MSQWKRISLLIVFTLVFGIIAFFHESRLGKWIDNEVYEFIYSSE
                     SFITTSIMLGATKVGEVWAMLCISLLLVAYLMLKRHKIEALFFALTMALSGILNPVLK
                     NIFDRERPTLLRLIDITGFSFPSGHAMGSTAYFGSGIYLLKRLNQGNSKGILIGLCAA
                     MILLISISRVYLGVHYPTDIIAGIIGGVFCIILSTLLLRNKLIN"
BASE COUNT        17789 a         7616 c         8068 g        11390 t
ORIGIN      
        1 gcataaataa attgttcaac acatagttgt aatgtgtttc aatacttttt ggatagattg
       61 cgaaattgta ttgaatcgtc atcgttttaa atttttaaat gagaatggaa tgagcattac
      121 aatacacaag caatcaaaag taaatacatt cacaacacaa cagagacata acaacaagat
      181 aaggagtgaa caatagctgt gaattatcgt gataaaattc aaaagtttag tattcgtaaa
      241 tataccgttg gtacattttc aacggtcatt gcgacattgg tatttttagg attcaataca
      301 tcacaagcac atgctgctga aacaaatcaa ccagcaagcg tggttaaaca gaaacaacaa
      361 agtaataatg aacagactga gaatcgagaa tctcaagtac aaaattctca aaattcacaa
      421 aatggtcaat cattatctgc tactcatgaa aatgagcaac caaatattaa tcaaacaaac
      481 catgtagatg taagtgcaga acaatcatct acaactgaaa atgaacaagc agtatctcaa
      541 aataagcaaa caaagaaaga tacagcagat gctacgccaa cacaatcggc taaagcggaa
      601 agcaagcatg aacaaaatga aagtcgttct gctaacaaaa aagaaaacga caataaagcg
      661 gctcatgtag aaaatcatga agcaaatgta gtcacagctt cagattcacc tgataatggt
      721 aacgtacaac atgaccgaaa tgaactacaa gcattttttg atgcaaatta tcatgattat
      781 cgctttattg accgcgaaaa tgcagattct gggacattta actatgtaaa aggcattttt
      841 gacaagatta atacgttatt aggaagcaat gatccaataa acaataaaga cttgcaactt
      901 gcatataaag aattggaaca agctgttgca ttgatccgca ctatgcctca acgtcaacaa
      961 actagccgac gctcaagcag aattcaaacg cgttcagttg agtcaagagc tgcagaacct
     1021 agatcagtat caagttatca aaatgcagac tcttcatact acgttgaaaa tgctaatgac
     1081 ggttcagggt atcctgtagg tacatatatt aatgcatcaa acaaaggtgc gccattctat
     1141 cttcctacag ccccatggaa tactttgaaa gcatcagctg caaaagaaat agtactgatc
     1201 acagcgaaac aaactggaga cggatatcaa tgggtaatta agtttaacaa aggtcatgca
     1261 ccacatgaaa atatgatttt ctggtttgct ttaccgtcag atcaagtgcc agttggacgt
     1321 actgactttg taacagtgaa tgcagacggg acgaatgtac aatggagtaa tggtgcggga
     1381 gctggtgctt acaaaccact tccacaaatg tggccatatg gtgtaaatga ctcacgttcg
     1441 cgagacttta aaattagaaa tagaagcgga caagttattt atgattggac tgatgttcat
     1501 gtaaatacat tgagagactt agcgcgagca ggagattatt tcagtgaagc tggagcaccc
     1561 gctgcgacta aagctatagg ggaacaaacg tttagatata ttaatggtga aagaccaaca
     1621 gaatcccctg gtgcatcaaa agtttatact tttattggtg ctggcgatgc aagttatacg
     1681 atttcattta aaacacaagg accaacaacg aacaaattgt attatgcagc cggtggtcgt
     1741 gctctagaat acaatcaatt atttatgtat agccaactat acgtagaatc aacgcaagac
     1801 caccaacaac gacttgatgg ttttagacaa gtagttaatc gttcttatcg tttagataca
     1861 acaaaaagag tagaagtaag tcagggtaac attcaaacta aaaaagtact tgaaagtaca
     1921 aacctaaata tcgatgactt tatggatgat ccattaagtt atgttaagac accgagtaat
     1981 aaagtgttag gtttttatcc aacaaatgcg aatcccaatg catatagaca tggtggaact
     2041 aatccattaa atgaatatca attaagtcag ttatttactg atgataaact acaacaggca
     2101 gctagaactg gcaatccaat acgattaatg attggatttg actatccaga tggtcatggc
     2161 aacgcagaaa ctttagtacc agttaacttg acggtattac ctgaaattca acataatatt
     2221 aaattttata aaaatgatga cacacaaaat attgccgata aaccagcttc aagacaagca
     2281 ggacatccaa tcttctatgt atatgcaggt aatcaaggga atgcttcagt gaatttaggt
     2341 ggaagcgtaa catctattca accgttacgt attaatttaa caagtagtga aaattttaca
     2401 gatagagatt ggcaaataac aggtattcca cgtacgttac acattgaaaa ctcgacaaat
     2461 agacctaata atgccagaga acgtaacatt gaattggttg gtaatttatt accgggtgac
     2521 tattttggaa caatacgttt tggccgtaaa gaacaattat tcgaaatacg tgttaagcca
     2581 catacaccaa caattactac aactgctgaa caactaagag gtacagcatt acaaaaagtg
     2641 ccaattacag ttacgaatat tccgttggac ccatcgtcat tagtttatat cgtttatcca
     2701 acagatcaaa caagagatgg tggatctgaa gcagaccaaa tgccttcaaa ctattcaata
     2761 ctggcgactg gtacacctga tggtgtgcat aataccataa caatacaacc aaaagactat
     2821 actgtgttta ttcctccggt aggtaaacaa attagagcat tgatttatta caataaagtt
     2881 gttgcttcta atatgagtaa tgcggttact attttgccgg atgatattcc gccgacaatt
     2941 aataatccag ttggcttaaa tgcaaagtat tatcgtggtg atgaagtcaa ctttacaatg
     3001 ggagtttcag atagacattc tggaataaaa aatacaacta ttactacttt accgaatggt
     3061 tggacatcaa atttaactaa agcggataag aataatggct cattatctat tactggtaga
     3121 gtgagtatga atcaggcatt taacagtgat attacattta aagtgtcagc gacagataat
     3181 gtcaataata cgacaaatga tagtcaatct aaacatgtga cagtacatgt aggtaaaatt
     3241 agtgaagatg cgcatccaat tgtattagca aattctgaaa aagttgtcgt tgtaaatccg
     3301 acatctgtat ctaatgatga aaaacaacga ataattactg catttatgaa taaaaaccaa
     3361 aatataagag gttatttagc ttcatcaaat ccagttacag ttgataataa tggtaatgtc
     3421 acattacatt accgtgatgg ctcatcaaca acgcttgatg ctacaaatgt gatgacatac
     3481 gaaccagttg tgaaacctga atatcaaact gccaatgcac ctaaaacggc aacggtaaca
     3541 attgctaaag gacaatcatt tagtataggt gatattaaac aatatttcac tttaagtaat
     3601 ggacaagcga ttccaaatgg aacatttaca aatattactt cggacagaac gattccaaca
     3661 gcacaagaag ttagtcaaat gaatgcaggt acacagttat accatatcgt cgcttcaaac
     3721 gcgtatcata aagacactga agacttatat attagtttga aaatcatcga tgtgaaacaa
     3781 ccagagggtg atcaacgtgt atatcgtaca tcaacatatg atttaactac tgatgaaatt
     3841 tcaaaagtaa aacaagcatt tattaatgcc aatagggatg cgattacgtt tgcagaagga
     3901 gatatatcgg ttacaaatac accaaatggt gccaatgtga gtaccgttac tgtcaatatt
     3961 aataaaggtc gattaacgaa atcgtttaca tcaaaccttg ctaatatgaa tttcttacgt
     4021 tgggttaatt tcccacaaga ttatactgta tcttggacaa atgcgaaaat cgctagtaga
     4081 ccaactgatg gtggtttatc atggtctgat gatcataaat cattaattta tcgctatgat
     4141 gcaacattag gtacaccaat tacgacgaat gatattttaa cgatgttaaa agcaacaacg
     4201 acagtgcctg gattgcgtaa taacattgcc ggtaatgaga aaactcaagc tgaagctggt
     4261 ggcagaccaa actatagacc aactggatat tcacaagcaa atgcttcatc agatgggcaa
     4321 cgtcaatata cgttaaatgg acaagtaatc caagttctag atattattaa cccgacgaat
     4381 ggtttcggtg gtcaaacgat tacaaactca aatgttcgtg caaaccatag taattcaact
     4441 gttgttagtg tcaatgaacc ggcagctaat ggtgcgggtg catttacaat tgatcatgtg
     4501 gtaaaaaata atactacgca taatgcagct gatgcggtat acaaagcgca gttatactta
     4561 tctccatatg gaccaaaaca atatgttgaa catttaaatc aaaatacggg aaatactacc
     4621 gatgctatta acatttattt tgtacctagt gatctagtaa atccaactat ttcagtaggt
     4681 aattacgcaa atcatcaagt attctctggt gaaacattta ccaatacgat tacagcgaat
     4741 gataactttg gtgtgcaatc tgtaactgta ccaaatacat cacaaattac aggtactgtt
     4801 gacaataatc atcagcacgt ttctgcaaca gcgccaaatg tgacatctgc tacaaataaa
     4861 acgatcaatt tagtggcaac tgatacaagc ggcaatactg caacaacgtc atacaatgtc
     4921 acaattaaac ctttacgtga taaatatcga gttggaacat catcaacggc tgctaatcca
     4981 gtgagaattg ctaatatttc gaataatgcg atagtatcac aagctgatca aacagcaatt
     5041 attaattcat tgacgtttac tgaaacagta ccaaatagaa gttatgcaag agcaagtgca
     5101 aatgaaatca ctagtaaaac agttagtaat gtgagtcgta ctggaaataa tgccaatgtc
     5161 acagtaactg ttacttatca agatggaaca acatcaacag tgactgtacc tgtaaagcat
     5221 gtcattccag aaatcgttgc acattcgcat tacactgtac aaggccaaga cttcccagca
     5281 ggtaatggtt ctagtgcatc agattacttt aagttatcta atggtagtgc cattccagat
     5341 gcaactatta catgggtaag tggacaagcg ccaaataaag ataatacgcg tattggtgaa
     5401 gatataacag taactgcaca tatcttaatt gatggcgaaa caacgccgat tacgaaaaca
     5461 gcaacatata aagtagtaag aactgtaccg aaacatgtct ttgaaactga cagaggtgtt
     5521 ttatacccag gtgtttcaga tatgtatgat gcaaaacaat atgttaagcc agtgaataat
     5581 tcttggtcga caaatgcgca aaatatgaat ttccaatttg tgggaactta tggacctaac
     5641 aaagatgttg taggtatatc aacgcgtctt attagaatca catatgataa tagacaaact
     5701 gaagaattaa ctattttatc taaagttaaa ccagacccac cgagaattga tggcaactct
     5761 gtaacatata aagcaggtct tacaaaccaa gaagttaaag ttaataacgt attaagtaac
     5821 tcgacggtta aattgtttaa agctgacaat acgccattaa atgttacaaa tattatccat
     5881 ggcagtggct atagttcggt tgtgacagta agtgatgcgt taccaaatgg tggaattaaa
     5941 gcgaaatctt caatttcaat gaacaatgtg acgtatacga cgcaagacga acatggtcaa
     6001 gttgttacag taacaagaaa tgaatctgtt gattcaaatg atagtgcttc tgttacagta
     6061 acaccacaat tacaagcaac tactgaaggc gctgtattta ttaaaggtgg cgacggtttt
     6121 gatttcggac acgtagaaag atttattcaa aacccaccac atggcgcaac ggtcgcatgg
     6181 cacgataatc cagatacatg gaagaataca gtcggtaaca ctcataaaac tgcggttgta
     6241 acattaccaa gtggtcaagg tacgcgtaat gttgaagttc cagtcaaagt ttatccagtt
     6301 gctaatgcta aggcgctatc acgtgatgtg aaaggtcaaa atttgactaa tggaacggat
     6361 gcgatgagct acattacatt tgatccaaat acaaacacaa atggtatcac tgcagcatgg
     6421 gcaaatagac aacaaccaaa taaccaacaa gcaggcgttc aacatttaaa tgtcgatgtc
     6481 acatatccgg gtatttcagc tgctaaacga gttcctgtaa ctgtgaacgt atatcaattt
     6541 gaattccctc aaactactta taccacaaca gttggtggca ctttagcaag tggtacgcaa
     6601 gcatcgggat atgcacatat gcaaaacgct actggtttac caactgatgg atttacgtat
     6661 aaatggaata atgcagcaac gggtacaaac gatgcaaact gggcagcaat gaataaaccg
     6721 aatgcggcta aagtcgttaa tgcgaaatat gatgttatct ataatggaca tacatttgca
     6781 acatctctac cagcgaagtt tgtagtaaaa gatgtacaac cagcgaaacc aactgtgact
     6841 gaaacagcgg caggagcgat tacaattgca cctggagcaa accaaacagt gaatacacat
     6901 gccggtaacg taacaacata cgctgataaa ttagttatta aacgtaacgg cagtgttgtg
     6961 acgacattta cacgtcataa taatacgagt ccatgggtta aagaagcatc agcaactaat
     7021 gtaacaggta ttgttggaac gaataatggt attactgttg cagcaggtac tttcaatcct
     7081 gctgatacaa ttcaagttgt tgcaacacaa ggtagtggtg aaacaatcag tgacgaacaa
     7141 cgtagtgatg atttcacagt tgtcgcacct caaccgaacc aagcgactac taagatttgg
     7201 caaaatggtc atattgatat cacgcctaat aatccatcag ggcatttaat taatccaact
     7261 caagcaatgg atattgctta cactgaaaaa gtgggtaatg gtgcagaaca tagtaagaca
     7321 attaatgttg ttcgtggtca aaataatcaa tggacaattg cgaataagcc tgactatgta
     7381 acgttagatg cacaaactgg taaagtgaca ttcaatgcca atactatcaa gccaaattca
     7441 gcaattacca ttacaccgaa agcaggtaca ggtcactcag caagcagtaa tccaagtaca
     7501 ttaactgcac cggcagcaca tactgtcaac acaacagaaa ttgtgaaaga ttatggttca
     7561 aatgtaacag catctgaaat taacaatgca gttcaagttg ctaataaacg tactgcaacg
     7621 attaaaaatg gcacagcaat gcctactaat ttagctggtg gtagcacaac gacgattcct
     7681 gtgacagtaa catacaatga tggcagtact gaagaagtac aagagtccat tttcacaaaa
     7741 gcggataaac gtgagttaat cacagctaaa aatcatttag atgatccagt gagtacagat
     7801 ggtaaaaagc caggtacaat tacgcagtac aataatgcaa tgcataatgc gcaacaacaa
     7861 atcaatgctg cgaaaacaga agcgcaacaa gtgattaata atgagcgtgc aacaccacaa
     7921 caagtgaacg cagcattatc taaagtccaa gcagcacaaa ctaagataaa tgaagctaaa
     7981 gctttacttc aaaataaaga agataatagc caattagtaa cgtctaaaaa taacttacaa
     8041 agttctgtga accaagtacc atcaactgcg ggtatgacgc aacaaagtat tgataactat
     8101 aatgcgaaga aacgtgaagc agaatcggaa attacagctg cacaacgtat cattgacaat
     8161 ggagatgcaa ctgcacaaca aatttcagat gaaaaacatc gtgtcgataa cgcattaaca
     8221 gcattaaacc aagcgaaaca tgatttaact gcagatacac atgccttaga gcaagcagtg
     8281 caacaattga atcgcacagg tacaacgact ggtaagaagc cagcaagtat cactgcgtat
     8341 aataattcaa ttcgtgcact tcaaagtgac ttaacaagtg ctaaaaatag cgctaatgct
     8401 atcattcaga agccaataag aacagtgcaa gaggtacaat ctgcattaac aaatgtaaat
     8461 cgtgtcaatg agcgattaac gcaagcaatt aatcaattag tacctttagc tgataatagt
     8521 actttaagaa ctgctaagac gaaacttgat gaagaaatta ataaatcagt aactactgat
     8581 ggtatgacac aatcatcaat ccaagcatat gaaaatgcta aacgtgcggg tcaaacagaa
     8641 tcaacaaatg cacaaaatgt tattaacaat ggtgacgcga cagaccaaca aattgccgca
     8701 gaaaaagcta aagtagaaga aaaatataat agcttaaaac aagcaattgc tggattaaca
     8761 ccagacttgg caccattaca aactgcaaaa acgcagttgc aaaatgatat tgatcagcca
     8821 acgagtacga ctggtatgac aagcgcatct gttgctacat ttaatgaaaa actttcagca
     8881 gctagaacta aaattcaaga aattaatcgc gtactggcat ctcatccaga tgttgcaacg
     8941 attcgtcaaa acgtgacagc agcgaatgct gctaaatcag cacttgatca agcacgtaat
     9001 ggcttaacag tcgataaagc gcctttagaa aatgcgaaaa atcaactaca acatagtatt
     9061 gatacgcaaa caagtacaac tggtatgacg caagattcta ttaatgcata caatgcgaag
     9121 ttaacagctg cacgtaataa gattcaacaa atcaatcaag tattagctgg ttcaccgaca
     9181 gttgatcaaa ttaatacaaa tacgtctgca gcaaaccaag ctaaatctga tttggatcat
     9241 gcgcgtcaag cattaacacc agataaaaca ccgcttcaaa atgcgaaaac gcaattagaa
     9301 caaagtatta atcaaccaac agatacaaca ggtatgacga ccgcttcgtt aaatgcgtac
     9361 aaccaaaaat tacaagcagc gcgtcaaaag ttaactgaaa ttaatcaagt gttgaatggc
     9421 aacccaactg tccaaaatat caatgataaa gtggcagagg caaaccaagc taaggatcaa
     9481 ctaaatacag cacgtcaagg tttaacatta gatagacagc cagcgttaac aacattacat
     9541 ggtgcatcta acttaaacca agcacaacaa aataatttca cgcaacaaat taatgctgct
     9601 caaaatcatg ctgcgcttga aacaattaag tctaacatta cggctttaaa tactgcgatg
     9661 acaaaattaa aagaaagtgt tgcggataat aattctatta aatcaggtca aaactacact
     9721 gacgcaactc aagctaataa acaagcgtat gataatgcag ttaatgcggc taaaggtgtc
     9781 attggggaaa cgaataatcc aacgatggat gttaatacag tgaaccaaaa agcagcatct
     9841 gttaaatcga cgaaagatgc tttagatggt caacaaaact tacaacgtgc gaaaacagaa
     9901 gcaacaaatg cgattacaca tgcaagtgat ttaaaccaag cacaaaagaa tgcattaaca
     9961 caacaagtga atagtgcgca aaatgttcaa gcagtaaata atattaaaca aacaactcaa
    10021 agcttaaaca ctgcaatgac aggtctaaaa cgtggcgttg ctaatcataa ccaagtcgta
    10081 caaagtgata attatgtcaa cgcagatact aataagaaaa atgattacaa caatgcatac
    10141 aaccatgcga atgacattat taatggtaat ccacaacatc cagttataac accaagtgat
    10201 gttaacaatg ctttatcaaa tgtcacaagt aaagaacatg cattgaatgg tgaagctaag
    10261 ttaaatactg cgaaacaaga agcgaacact gcattaggtc aattaaataa cttgaataat
    10321 gcacaacgtc aaaacttaca atcgcaaatt aatggtgcgc atcaaatcga gacagttaat
    10381 acgattaagc aaaatgcaac taacttaaat agtgcaatgg gtaatttaag acatgctgtt
    10441 gcagataaag agcaagtcaa acgtacagaa gattatgcgg atgcagatac agctaaacaa
    10501 aatgcatata atagtgccgt ttcaagtgct gaaacaatca ttaatcaatc aacaaatccg
    10561 acaatgtctg ttgatgatgt taatcgtgca acttcagctg ttactactaa taaaactgca
    10621 ttaaatggtg atgaaaaatt agcacaatct aaaacagatg ctgcaagagc aattgatgca
    10681 ttaccacatt taaataatgc acaaaaagca gatgttaaat ctaaaattaa tgctgcatca
    10741 aatattgctg gcgtaaatac tgttaaacaa caaggtacag atttaaatac agcaatgggt
    10801 aacttgcaag gtgcgatcaa tgatgaacaa acgacactta atagtcaaaa ttatcaagat
    10861 gcgacaccta gtaagaaaac agcatacaca aatgcggtac aagctgcgaa agatatttta
    10921 aataaatcaa atggtcaaaa taaaacgaaa gatcaagtta ctgaagcgat gaatcaagtg
    10981 aattcggcta aaaataactt agatggtacg cgtttattag atcaagcgaa gcaaacagcg
    11041 aaacagcagt taaataatat gacgcattta acaactgcac aaaaaacgaa tttaacaaac
    11101 caaattaata gtggtactac tgtcgctggt gttcatacgg ttcaatcaaa tgccaataca
    11161 ttagatcaag ccatgaatac gttaagacaa agtattgcca acaaagatgc gactaaagca
    11221 agtgaagatt acgtagatgc taataatgat aagcaaacag catataacaa cgcagtagcc
    11281 gctgctgaaa cgattattaa tgcaaatagt aatccagaaa tgaatccaag tacgattaca
    11341 caaaaagcag agcaagtgaa tagttctaaa acggcactta acggtgatga aaacttagct
    11401 acggcaaaac aaaatgcgaa aacgtactta aacacattaa cgagtattac agatgctcaa
    11461 aagaacaatt tgattagtca aattagtagt gcgacaagag tgagtggtgt tgatactgta
    11521 aaacaaaatg cgcaacatct agaccaagct atggctagct tacaaaatgg tattaataac
    11581 gaatctcaag ttaaatcttc tgagaaatat cgtgatgctg atacaaataa acaacaagag
    11641 tatgataatg ctattactgc agcgaaagcg attttaaata aatcgacagg tccaaacact
    11701 gcgcaaaatg cagttgaagc agcattgcaa cgtgtgaata ctgcgaaaga tgcattgaat
    11761 ggtgatgcaa aattaattgc agctcaaaac gcagcaaaac aacatctagg tactttaaca
    11821 catatcacta cagcacaacg taatgattta acaaatcaaa tttcacaagc tacaaactta
    11881 gctggtgttg aatcagttaa acaaagtgca aatagtttag atggcgcaat gggtaactta
    11941 caaactgcta tcaacgataa atctggaaca ttagcgagcc aaaacttctt ggatgctgat
    12001 gagcaaaaac gtaatgctta caatcaagct gtatcaaatg ccgaaacaat tttaaataaa
    12061 caaactggac cgaatacagc gaaaactgca gttgaacaag cacttaataa tgttaatagt
    12121 gcgaaacatg cattaaatgg tacgcaaaac ttaaataatg cgaaacaagc agcgattaca
    12181 gcaatcaatg gcgcatctga tttaaatcaa aaacaaaaag atgtattaaa agcacaagct
    12241 aatggtgctc aacgcgtttc taatgcacaa gatgtacagc gcaatgcgac tgaactgaac
    12301 acggcaatgg gtacattaaa acatgccatc gcagataaga cgaatacgtt agcgagcagt
    12361 aaatatgtta acgccgatag cactaaacaa aatgcataca caactaaagt taccaatgct
    12421 gaacatatta ttagcggtac gccaacggtt gttacgacac cttcagaagt aacagctgca
    12481 gctaatcaag taaacagcgc gaaacaagaa ttaaatggtg acgaaagatt acgtgtagca
    12541 aaacaaaacg ccaatactgc tattgatgca ttaacgcaat taaatactcc tcaaaaagct
    12601 aaattaaaag aacaagtggg acaagccaat agattggaag acgtacaaac tgttcaaaca
    12661 aatggacaat cattgaacaa tgcaatgaaa ggcttaagag atagtattgc taacgaaaca
    12721 acagtcaaag caagtcaaaa ctatacagac gcaagtccga ataaccaatc aacatataat
    12781 agcgctgtgt caaatgcgaa aggtatcatt aatcaaacta acaatccaac tatggatact
    12841 agtgcgatta cccaagctac aacacaagtg aataatgcta aaaatggttt aaacggtgct
    12901 gaaaacttaa gaaatgcaca aaacactgct aagcaaaact taaatacgtt atcacactta
    12961 acaaataacc aaaaatctgc aatctcatca caaattgatc gtgcaggtca tgtgagtgag
    13021 gtaacagctg ctaaaaatgc agcaactgag ttaaacacgc aaatgggtaa cttggaacaa
    13081 gctatccatg atcaaaacac agttaagcaa agtgttaact ttactgatgc agataaagct
    13141 aaacgtgatg cgtatacaaa tgcggtaagc agagcagaaa caattctgaa taaaacgcaa
    13201 ggtgcaaata cttcaaaaca agatgttgaa gcagctattc aaagtgttac tagtgctaaa
    13261 aatgcattga atggtgatca aaatgtcaca aatgcgaaga atacagctaa acatgcatta
    13321 aataacttaa cgtctattaa taatgcacaa aaacgtaact taacaactaa aattgatcaa
    13381 gcaacaactg tagctggtgt agaagcggtt tctaatacag gtacgcaatt gaatacagcg
    13441 atggctaact tgcaaaatgg tattaatgat aaaacaaata cactagcgag cgaaaactat
    13501 catgatgctg attcagataa gaaaactgct tatactcaag ccgttacgaa cgcagaaaat
    13561 attttaaata aaaatagtgg atcaaattta gacaaagttg ccgttgaaaa cgcgttgtca
    13621 caagtaacaa atgcaaaagg tgccctaaat ggtaaccata atttagagca agctaaatca
    13681 aatgcaaaca ctactataaa cggacttcaa catttaacaa ctgcacaaaa agataaattg
    13741 aaacaacaag tgcaacaagc acaaaatgtt gcaggtgtag atactgttaa atcaagtgcc
    13801 aacacattaa atggtgctat gggtacgtta agaaatagca ttcaagataa cacaggtacg
    13861 aaaaatggcc aaaactatct tgatgctaca gaaagtaaca aaacaaacta taacaatgct
    13921 gttgatcgtg ctaatggtgt cattaatgca acaagcaatc caaatatgga tgctaatgca
    13981 attaaccaaa tcgctacaca agtgacatca acgaaaaatg cattagatgg tacacataat
    14041 ttaacacaag cgaaacaaac agcaacaaat gccatcgatg gtgctactaa cttaaataaa
    14101 gcgcaaaaag atgcgttgaa agcacaagtg acaagtgcgc aacgtgttgc aaatgtaaca
    14161 agtatccaac aaactgcaaa tgaacttaat acagctatgg gtcaattaca acatggtatt
    14221 gatgatgaaa atgcaacaaa acaaactcaa aaatatcgtg acgctgaaca aaataagaaa
    14281 actgcttatg atcaagctgt agctgctgcg aaagcaattt taaataaaca aactggatca
    14341 aattcagata aagcagcagt tgaccatgca ttacaacaag taacaagtac gaaagatgca
    14401 ttgaatggtg atgctaaact ggcagaagcg aaagcggcag ctaaacaaaa cttaggcact
    14461 ttaaaccata ttacgaatgc acaacgtact gcgttagaag gtcaaatcaa tcaagcgacg
    14521 actgttgatg gcgttaatac tgtgaaaaca aatgcaaata cattagacgg cgcaatgaat
    14581 agcttgcaag gttcaatcaa tgataaagat gcgacattaa gaaatcaaaa ttatcttgat
    14641 gcagatgaat caaaacgaaa tgcatatacg caagctgtca cagcggctga aggcatttta
    14701 aataaacaaa ctggtggtaa cacatctaaa gcagacgttg ataatgcatt aaatgcagtt
    14761 acaagagcga aagcggcttt aaatggtgct gacaacttaa gaaatgcgaa aacttcagca
    14821 acaaatacga ttaatgggtt accacactta acacaattac aaaaagacaa cttgaagcat
    14881 caagttgaac aagcgcaaaa tgtagctggt gtaaatggtg ttaaagataa aggtaatacg
    14941 ttaaatactg ccatgggtgc attacgtaca agtatccaaa atgataatac gacgaaaaca
    15001 agtcaaaatt atcttgatgc atctgatagc aacaaaaata attacaatac tgctgtaaat
    15061 aatgcaaatg gtgttattaa tgcaacgaac aatccaaata tggatgctaa tgcgattaat
    15121 ggcatggcaa atcaagtcaa tacaacaaaa gcagcgttaa atggtgcaca aaacttagct
    15181 caagctaaaa caaatgcgac gaacacaatt aacaacgcac atgacttaaa ccaaaaacaa
    15241 aaagatgcat taaaaacaca agttaacaat gcacaacgtg tatctgatgc aaataacgtt
    15301 caacgtacag ctactgaatt gaacagtgcg atgacagcac ttaaagcagc tattgctgat
    15361 aaagaaagaa caaaagcaag cggtaattat gtcaatgctg atcaagaaaa acgtcaagcg
    15421 tatgattcaa aagtgactaa cgctgaaaat attattagtg gtacaccgaa tgcgacatta
    15481 acagtcaatg acgtaaatag tgcggcatca caagtcaatg cggctaaaac agcattaaat
    15541 ggtgataaca acttacgtgt agcgaaagag catgccaaca atacaattga tggcttagcg
    15601 caattgaata atgcacaaaa agcaaaatta aaagaacaag ttcaaagtgc aactacatta
    15661 gatggcgttc aaactgttaa aaatagttct caaacgttga atacagcgat gaaaggctta
    15721 agagatagta ttgcgaatga agcaacaatt aaagcaggtc aaaactacac tgacgcaagt
    15781 ccaaataatc gtaacgagta cgacagtgca gttactgcag caaaagcaat cattaatcaa
    15841 acatcgaacc caacgatgga accaaatact attacgcaag caacatcaca agtgacaact
    15901 aaagaacatg cattaaatgg tgcgcaaaac ttagctcaag ctaagacaac tgcgaaaaac
    15961 aacttaaata acttaacatc aattaacaat gcacaaaaag atgcgttaac gcgtagcatt
    16021 gatggtgcaa caacagtagc tggtgtaaat caagaaactg ccaaagcaac agaattaaat
    16081 aacgcaatgc acagtttaca aaatggtatc aatgatgaga cacaaacaaa acaaactcag
    16141 aaatacctag atgctgagcc aagtaagaaa tcagcttatg atcaagctgt aaatgcagcg
    16201 aaagcaattt taacaaaagc tagtggtcaa aatgtagaca aagcagcagt tgaacaagca
    16261 ttacaaaatg tgaacagtac gaagacggcg ttgaacggtg atgcgaaatt aaatgaagct
    16321 aaagccgctg cgaaacaaac gttaggtaca ttaacacaca ttaataatgc acaacgtaca
    16381 gcgttagata atgaaattac acaagcaaca aatgttgaag gtgttaatac agttaaagcc
    16441 aaagcgcaac aattagatgg tgctatgggt caattagaaa catcaatacg tgataaagac
    16501 acgacgttac aaagtcaaaa ttatcaagat gctgatgatg cgaaacgaac tgcatattct
    16561 caagcagtaa atgcagcagc aactatttta aataaaacag ctggcggtaa tacacctaaa
    16621 gcagatgttg aaagagcaat gcaagctgtt acacaagcaa atactgcatt aaacggtatt
    16681 caaaacttag aacgtgcgaa acaggctgct aacacagcga ttacaaatgc ttcggactta
    16741 aatacaaaac aaaaagaagc attaaaagca caagtaacaa gtgcaggacg agtatctgca
    16801 gcaaatggtg ttgaacatac tgcgactgaa ttaaatactg cgatgacagc tttaaaacgt
    16861 gccattgctg ataaagctga gacaaaagct agtggtaact atgtcaatgt tgatgcgaat
    16921 aaacgccaag catatgatga aaaagtgaca gctgcagaaa atatcgttag tggtacacca
    16981 acaccaacgt taacaccatc agatgttaca aatgcagcaa cacaagtaac gaatgcgaag
    17041 acgcagttaa acggtaatca taatttagaa gtagcgaaac aaaatgctaa cacagcaatt
    17101 gatggtttaa cttctttaaa tggtccgcaa aaagctaaac ttaaagaaca agtgggtcaa
    17161 gcgacgacgt tgccaaatgt tcaaactgtt cgtgataatg cacaaacatt aaacactgca
    17221 atgaaaggtc tacgagaaag cattgcgaat gaagcaacga ttaaagcaga tcaaaactac
    17281 acggatgcaa gtcaaagtaa acaaactgac tacaacaatg cagtcactgc agcaaaagca
    17341 atcattggtc aaacaactag tccaacaatg aatgcgcaag aaatcaatca agcgaaagac
    17401 caagtgacag ctaaacaaca agcgttaaac ggtcaagaaa acttaagaac tgcgcaaaca
    17461 aatgcgaagc aacatttgaa tggcttaagt gacttaacta atgctcaaaa agaagcagcc
    17521 aaacgccaaa tcgaaggtgc aacgcatgtt aatgaagtaa cacaagcgca aaataatgcg
    17581 gacgcattaa atacagctat gacgaacttg aaaaatggta ttcaagatca aaatacgatt
    17641 aagcgaggtg ttaacttcac tgatgcagat gaagcgaaac gtaatgcata tacaaatgca
    17701 gtgacgcaag ctgaacaaat tttaaataaa gcacaaggtc caaatactgc aaaagacggt
    17761 gtcgaaactg cgttacaaaa tgtacaacgt gctaaaaacg atttgaacgg taatcaaaat
    17821 gttgcgaatg ctaagacaac tgcgaaaaat gcattaaata atttaacatc aattaataat
    17881 gcacaaaaag aagcattgaa atcacaaatt gaaggtgcga caacagttgc aggtgtaaat
    17941 caagtgtcta caacggcatc tgaattaaat actgcaatga gcaacttaca acgtggtatc
    18001 aacgatgaag cagctacaaa agcagctcaa aaatatactg atgcagatag agaaaaacaa
    18061 acagcataca atgatgccgt aacagcagct aaaacgttat taaataaaac agctggttca
    18121 aatgataata aagcagccgt tgaacaagca ttacaacgtg tgaatactgc gaaaaatgca
    18181 ttaaatggtg atgcgcgctt aaatgaagcg aagaaaacag ctaaacaaca attagcgaca
    18241 atgtcacatt taactgatgc tcaaaaagca aacttaacat cgcaaattga acgcggtacg
    18301 actgttgcag gtgttcaagg tattcaagct aatgctggta ctttaaatca agcaatgaat
    18361 caattaagac aaagtattgc ttctaaagat gcgactaaat caagtgaaga ttatcaagac
    18421 gcgaatacag atttacaaaa tgcatacaac cgtgcagtat cagatgctga aggtattatt
    18481 agtgcaacga ataaccctga aatgaatcca gatacgatta atcaaaaagc gagccaagtg
    18541 aatagtgcga agtcagcatt gaacggtgac gaaaaattag cagccgcaaa acaaactgcg
    18601 aaaacagata tcggtcgttt aacagactta aacaatgccc aacgaactgc ggcaaatgca
    18661 gaagtggatc aagcaccaaa ccttgcagcg gtaacagcag ctaaaaataa agcgacatcg
    18721 ttaaatacag cgatgggtaa tttgaaacat gcacttgctg aaaaggataa tacgaagcga
    18781 agtgtcaatt acacagatgc ggatcgacca aaacaacaag cgtatgacac tgcagttaca
    18841 caagccgaag caattactaa tgccaatggc agcaacgcga atgaaacaca agttcaagca
    18901 gcgcttaacc aattgaatca agctaaaaat gacttgaatg gtgataataa agttgctcaa
    18961 gctaaagaag cagcgaaacg tgcattagct tcatataata acttgaataa tgcgcaatca
    19021 attgcagcaa ctagtcaaat tgacaatgca acgacagtag caggcgtaac tgctgcacaa
    19081 aatactgcta atgaattaaa tacagctatg ggtcaacttc aaaatagtat taatgaccaa
    19141 aacactgtta aacaacaagt gaactttaca gatgctgacc aaggtaagaa agatgcttac
    19201 acaaatgctg ttacgaatgc tcaaggtatt ttagataaag cacacggtca aaatatgaca
    19261 aaagcacaag ttgaagctgc attaaatcaa gtaacgactg ctaagaatgc tttaaacggt
    19321 gacgcaaatg taagacaagc aaaatcagat gcgaaagcaa acttaggtac attaacacac
    19381 ttaaataatg cacaaaaaca agatttaaca tcacaaatcg aaggcgcaac aacagtcaac
    19441 ggtgtaaatg gtgttaaaac gaaagcacaa gacttagatg gtgcaatgca acgattagaa
    19501 tcagcaatcg caaataaaga tcaaactaga gcgagcgaaa actacatcga cgcagatcca
    19561 actaagaaaa cagcatttga taatgccatc acacaagctg aatcttactt aaataaagat
    19621 catggtgcga ataaagataa gcaagctgtt gaacaagcaa ttcaaagtgt aacgactgct
    19681 aagaatgctt taaacggtga cgcgaactta caacgcgcta aaactgaagc tacacaagct
    19741 atcgataact tgacacattt gaatacacca caaaaaacag cgttaaaaca acaagtgaac
    19801 gctgcgcaac gtgtatcagg tgtaactgat ctgaaaaata gtgctacatc acttaataat
    19861 gcgatggatc aattaaaaca agcaattgct gatcatgaca caattgttgc tggtggtaat
    19921 tacactaatg caagtcctga taagcaaggt gcttatactg atgcatataa tgctgcgaaa
    19981 aacattgtaa atggttcacc taatgtggtt acaaatgcag cagatgttac agcagcaaca
    20041 caacgtgtta ataatgctga aacaggttta aatggtgatt caaacttagc aactgcgaag
    20101 caacaagcta aagatgcatt acgtcaaatg acgcatttat ctgctgcaca aaaacaaagt
    20161 attactggtc aaattgatag tgcgacacaa gtaactggcg ttcaaagtgt aaaagacaat
    20221 gcaacaaatc ttgacaatgc aatgaatcaa cttcgaaata gtattgcgaa taaagatgaa
    20281 gttaaagcga gtcagccata tgttgatgca gatacagata aacaaaatgc atacaacaca
    20341 gctgttacaa gtgctgaaaa tatcattaat gcaacgagtc agccgacact tgatccatct
    20401 gcagtaacac aagcagctaa tcaagtgaac actaacaaaa ctgcgcttaa tggtgtacaa
    20461 aacttagcga ataaaaagca agaaacgact gctaacatca accaattaag tcatttaaat
    20521 aatgctcaaa agcaagattt aaatacgcaa gtgacaaatg caccaaatat tagcacagta
    20581 aatcaagtga aaactaaagc tgaacaatta gatcaagcaa tggaacgttt aatcaacgga
    20641 atccaagaca aagatcaagt gaaacaaagt gttaacttta cagatgcaga tccagaaaaa
    20701 caaacagcat acaacaatgc ggtaacggct gctgaaaata ttattaatca agcaaatggt
    20761 acaaatgcga accaatcaca agttgaagca gcactttcaa ctgtaacaac tactaaacaa
    20821 gcgttgaatg gtaatagaaa agtaacagat gctaaaaaca atgcaaacca aacattatct
    20881 acattagata acttaaacaa tgcacaaaaa ggtgctgtta ctggaaacat caatcaagcg
    20941 cacactgtag ctgaagtaac gcaagccatt caaacagctc aggaactgaa tacagcgatg
    21001 ggtaacttga aaaatagctt gaatgataaa gacactacac ttggcagtca aaactttgca
    21061 gatgcagatc cagagaagaa aaatgcatac aatgaagcgg ttcataatgc tgaaaatatt
    21121 ttaaataaat ctacaggtac aaacgtgcct aaagatcaag ttgaagcagc tatgaatcaa
    21181 gtgaatacta caaaagcagc gcttaatggt actcaaaacc ttgaaaaagc taaacaacac
    21241 gcaaatacag caattgacgg tttaagccat ttaacaaatg cacaaaaaga tgcattaaaa
    21301 caattggtac aacaatcgac tactgttgca gaagcacaag gtaatgaaca aaaagcaaac
    21361 aatgttgatg cagcaatgga caaattacgt caaagtattg cagataatgc gacaacaaaa
    21421 caaaaccaaa attatactga tgcaagtccg aataaaaagg atgcgtacaa taatgctgtc
    21481 acaactgcac aaggtattat tgatcaaact acaagtccaa ctttagatcc gactgttatc
    21541 aatcaagctg ctggacaagt aagcacaact aaaaatgcat taaatggtaa tgaaaaccta
    21601 gaggcggcga aacaacaagc gacacaatca ttaggttcat tagacaactt aaataatgcg
    21661 caaaaacaaa cagttactga tcaaattaat ggcgcgcata ctgttgatga agcaaatcaa
    21721 attaagcaaa atgcgcaaaa cttaaataca gcgatgggta acttgaaaca agcgattgct
    21781 gacaaagatg ctacgaaagc aacagtaaac ttcactgatg cagatcaagc aaaacaacaa
    21841 gcatataaca ctgctgttac aaatgctgaa aacattattt caaaagctaa tggtggcaat
    21901 gcaacacaaa ctgaagttga acaagcaatc caacaagtaa atgcagcaaa acaagcatta
    21961 aatggtaatg ccaacgttca acatgcaaaa gacgaagcaa cagcattaat taatagctct
    22021 aatgacctta accaagcaca aaaagacgca ttaaaacaac aagttcaaaa tgcaactact
    22081 gtagctggtg taaacaatgt taaacaaaca gcacaagagt taaacaatgc gatgacacaa
    22141 ttaaaacaag gcattgcaga taaagaacaa acaaaagctg atggtaactt tgtcaatgca
    22201 gatcctgaca agcaaaatgc atataatcaa gcagtagcaa aagctgaagc attaattagt
    22261 ggtacgcctg atgtagtcgt tacacctagc gaaattactg cagctttaaa taaagttacg
    22321 caagctaaaa atgatttgaa tggtaatacg aacttagcaa cggcgaaaca aaatgttcaa
    22381 caagctattg atcaattgcc aaacttgaat caagcgcaac gtgatgaata caacaaacaa
    22441 attacgcaag caactcttgt accaaacgtc aatgctattc aacaagcggc gacaacgctt
    22501 aatgacgcga tgacacaatt gaaacaaggt attgcgaata aagcacaaat taaaggtagc
    22561 gagaactatc acgatgctga tactgacaag caaacagcat atgataatgc agtaacaaaa
    22621 gcagaagaat tgttaaaaca aacaacaaat ccaacaatgg atccaaatac aattcaacaa
    22681 gcattaacta aagtgaatga cacaaatcaa gcacttaacg gtaatcaaaa attagctgat
    22741 gctaaacaag cagctaagac aaatcttggc acattagatc atttaaatga tgctcaaaaa
    22801 caagcgttaa caacacaagt tgaacaagca ccagatattg caacagttaa taatgttaag
    22861 caaaatgctc aaaatctgaa taatgctatg actaacttaa acaatgcatt acaagataaa
    22921 actgagacat taaatagcat taactttact gatgcagatc aagctaagaa agatgcttat
    22981 actaatgcgg tagcacatgc agaaggtatt ttatctaaag caaatggcag caatgcaagt
    23041 caaactgaag tggaacaagc gatacaacgt gtgaacgaag cgaaacaagc attgaatggt
    23101 aatgacaatg tacaacgtgc aaaagatgca gcgaaacaag taattacaaa tgcaaatgat
    23161 ttaaatcaag cgcaaaaaga tgcattaaaa caacaagtcg atgctgcgca aactgttgca
    23221 aatgtaaaca caattaagca aacagcacaa gatttaaatc aagcaatgac acaattgaag
    23281 caaggtattg cagataaaga ccaaactaaa gcaaatggta actttgtcaa tgcagatact
    23341 gataagcaaa atgcatataa caatgcggta gcacatgctg aacaaatcat tagtggtaca
    23401 ccaaatgcaa acgtggatcc acaacaagtg gctcaagcgt tacaacaagt gactcaagct
    23461 aagggtgatt taaacggtaa ccataactta caagttgcta aagacaatgc gaatactgcc
    23521 attgatcagt taccaaactt aaatcaacca caaaaaacag cgttaaaaga ccaagtgtca
    23581 catgcagaaa ttgttacagg tgttaatgct attaagcaaa atgctgatgc attaaataat
    23641 gcaatgggta cgttgaaaca acaaattcaa gcaaacagtc aagtaccgca atcagttgac
    23701 tttacacaag cggatcaaga caaacaacaa gcatataaca atgcagctaa ccaagcgcaa
    23761 caaattgcaa atggaacacc aacacctgta ttgtcgcctg atacagtaac acaagcagtt
    23821 acaactatga atcaagcgaa agatgcatta aacggtgatg aaaaattagc gcaagcgaaa
    23881 caggatgctt tagcaaatct ggatacgtta cgtgacttaa atcaaccaca acgtgatgcg
    23941 ttacgtaatc aaatcaacca agcacaagcg ttagctacag tcgaacaaac taaacaaaat
    24001 gcacaaaatg tgaatgcaac aatgggtaac ttgaaacaag gtattgcaaa taaagatact
    24061 gtcaaagcaa gtgagaacta tcatgatgct gatgccgata agcaaacagc atatacaaat
    24121 gcagtgtctc aagcggaagg tattatcaat caaacgacaa atccaacgct taatccagat
    24181 gaaataacac gtgcattaac tcaagtaact gatgctaaaa atggcttaaa cggtgaagct
    24241 aaattagcaa ctgaaaagca aaatgctaaa gatgccgtaa gtggaatgac gcatttaaac
    24301 gatgctcaaa aacaagcatt aaaaggtcaa atcgatcaat cgcctgaaat tgctacagtg
    24361 aaccaagtta aacaaacagc aacgagccta gatcatgcaa tggatcaatt atcacaagct
    24421 attaatgata aagctcaaac attagcggac ggtaattact taaatgcaga tcctgacaaa
    24481 caaaatgcgt ataaacaggc agtagcaaaa gctgaagcat tattgaataa acaaagtggt
    24541 actaatgaag tacaagccca agttgaaagt atcactaatg aagtgaaaac agcgaaacaa
    24601 gcattaaatg gtaatgataa tttagcgaat gctaagcaac aagcgaaaca acaattggcg
    24661 aatttaacac acttaaatga tgctcaaaaa caatcatttg aaaatcaaat aacacaagcg
    24721 ccacttgtta cagatgtcac tacaattaat caaaaagcgc aaacgttaga tcatgcgatg
    24781 gaattattaa gaaatagtat tgcagataac caagcgacat tagcatctga agattatcat
    24841 gatgcaactg cgcaaagaca aaatgactat aaccaagctg taacagctgc taataatatc
    24901 attaatcaaa caacatcacc tacgatgaat ccagatgatg ttaatcgtgc aacaactcaa
    24961 gtgaataaca cgaaagttgc attagatggt gatgaaaacc ttgtagcagc taagcaacaa
    25021 gcgaacaata gacttgatca attagatcat ttaaataatg cgcaaaagca acagttacaa
    25081 tcacaaatta cgcaatcatc tgatattgca gcagttaatg gtcacaagca aactgcagaa
    25141 tctttaaata ccgcaatggg taacttaatc aatgcgattg cagatcatca agccgttgaa
    25201 caacgtggta acttcatcaa tgctgatact gataaacaaa ctgcttatac tacagcagta
    25261 aatgaagcag aagcaatgat taacaaacaa actggtcaaa atgcgaacca aacagaagta
    25321 gaacaagcta ttactaaagt tcaaacaaca cttcaagcgt taaatggaga tcataattta
    25381 caagttgcta aaacaaatgc gacgcaagca attgatgctt taacaagctt aaatgatcct
    25441 caaaaaacag cattaaaaga ccaagttact gctgcaacat tagtaactgc agttcatcaa
    25501 attgaacaaa ctgcgaatac gcttaaccaa gcaatgcatg gcttaagaga aagtattcaa
    25561 gataacgcaa caactcaagc aaatagcaaa tatatcaacg aagatcaacc agagcaacaa
    25621 aactacgatc aagcagtgca agctgcaaat aacattatca atgagcaaac agcgacatta
    25681 gataataatg cgattaatca agcagcaaca actgtgaata caacgaaagc agcattacat
    25741 ggtgatgtga agttacaaaa tgataaagat cgtgcgaaac aaacggttag tcaattagca
    25801 catctaaaca atgctcaaaa acatatggaa gatacgttaa ttgatagtga aacaactaga
    25861 acagccgtta accatgattt gactgaagca caagcattag atcaacttat ggatacatta
    25921 caacaaagta ttgctgacaa agatgcaaca cgtgcgagca gtgcctatgt caatgcagaa
    25981 ccgaataaaa aacaagcata tgatgaagca gttcaaaatg ctgagtctat cattgcagga
    26041 ttaactaatc caactatcaa taaaggcaat gtttcaagtg cgacacaagc agtaacatca
    26101 tctaaaaatg cattagacgg tgttgaacga ttagcgcaag ataagcaaac tgctggaaat
    26161 tctctaaatc atttagatca attaacgcca gcacaacaac aagcgctaga aaatcaaatt
    26221 aataatgcaa caactcgtga taaagtggct gaaatcattg cacaagcgca agcattaaat
    26281 gaagcgatga aagcattaaa agaaagtatt aaagatcaac cacaaactga agcaagtagt
    26341 aaatttatta atgaagatca agcacaaaaa gatgcttata cgcaagctgt acaacacgcg
    26401 aaagatttga ttaacaaaac aactgatcct acattagcta aatcaatcat tgatcaagcg
    26461 acacaggcag taactgatgc taaaaacaac ttacatggtg atcaaaaatt agctcaagat
    26521 aagcaacgtg caacagaaac gttaaataac ttgtctaact tgaatacacc acaacgtcaa
    26581 gcacttgaaa atcaaatcaa taatgcagca actcgtgacg aagtagcaca aaaattaact
    26641 gaagcacaag cacttaacca agcaatggaa gctttacgta atagcattca agatcaacag
    26701 caaacagaag cgggtagcaa gtttatcaat gaagataagc cgcaaaaaga tgcataccaa
    26761 gcagcagttc aacatgccaa agatttgatt aatcaaacta gcaatccaac gcttgataaa
    26821 gcacaagttg aacaattgac acaagctgtt aaccaagcta aagataacct acatggtgat
    26881 caaaaacttg cagacgataa acaacatgca gttactgatt taaatcaatt aaatggtttg
    26941 aacaatccgc aacgtcaagc gcttgaaagt caaataaaca atgcagcaac tcgtggtgaa
    27001 gtagcacaaa aattaactga agcacaagca cttaaccaag caatggaagc tttacgtaat
    27061 agcattcaag atcaacagca aacagaatct ggtagcaagt ttattaatga agataaaccg
    27121 caaaaagatg cttaccaagc agcagttcaa aatgcaaaag atttaatcaa ccaaacaggt
    27181 aatccaacac tcgacaaatc acaagtagaa caattaacac aagcagtaac aactgcaaaa
    27241 gataatctac atggtgatca aaaactagct cgtgatcaac aacaagcggt aacgactgtt
    27301 aatgcattac caaacttaaa tcatgcacaa caacaagcat taactgatgc aattaatgca
    27361 gcgcctacaa gaacagaggt tgcacaacat gttcaaactg ctactgaact tgatcacgcg
    27421 atggaaacat tgaaaaataa agttgatcaa gtgaaaacag ataaggccca accaaattac
    27481 actgaagcat cgacggataa aaaagaagca gtggatcaag cgttacaagc tgcacaaagc
    27541 attacagaac ctaataatgg ttcaaatgca aataaagatg ctgtagaaca agcattaact
    27601 aagcttcaag aaaaagaaaa tgagttaaat ggtaatgaga gagtcgctga agctaaaaca
    27661 caagcgaaac aaacagttga ccaattaaca catttaaatg ctgatcaaat tgcaactgca
    27721 aaacaaaaca ttgatcaagc gatgaaactt caaccaatcg cttaattagt agatcaagca
    27781 acgcaattga acaaatctat ggatcaatta caacaagcgg tcaatgatca tacaaacgtt
    27841 gagcaaactg tagattacac gcaagcagat tcagataagc aaaatgctta taaacaagct
    27901 attgcagatg ctgaaaatgt attaaaacaa aatgcgaata aggaacaagt ggatcaagca
    27961 cttcaaaaca ttttaaatgc aaaacaagca ttaaatggtg atgaacgcgt agcacttgct
    28021 aaaacaaatg gtaaacatga tattgaccaa ttaaatacat taaacaatgc acaacaagat
    28081 ggatttaaag gcagaattga tcaatctaat gacttaaatg agatcaaaca aattgtagat
    28141 gaagctaaag ctctaaatcg tgcaatgaat caattgtctc aagaaatcac tggcaatgaa
    28201 ggacgtacga aaggtagtac gaactatgtc aatgcagata cacaagttaa acgcgtgtac
    28261 gatgatgcag ttgataaagc gaaggaagca ctaaataaag cgacaggtca aaacttaact
    28321 gcagaagaag ttatcaagtt gaacgatgcg gtaacagcag cgaaacaggc attaaatggt
    28381 gaagaaagac ttaacaagcg taagtctgaa gcattacaaa gattggatca attaacacat
    28441 ctaaacaatg ctcaaagaca attagcaaca caacaaatta ataatgctga aacattaaat
    28501 aaagcgtctc gagcaattaa tagagctgtt caattagatg atgcaatggg tgcagtacaa
    28561 cagtatattg atgatcatca ttttgatgtc attagcagta caaattacat caatgcagac
    28621 gataacttga aagcaaatta tgataatgcc attacaaatg cagcgcatga gttagataaa
    28681 gttcaaggta gtgcaatcac gaaagctgag gcagaacaat taaaacaaaa tattatagat
    28741 gcgcaacaag ccttaaatgg tgatcaaaat cttgcaactg ctaaagataa agccaatgcc
    28801 tttgttgata cattaaatgg tttaaatcaa caacaacaag atcttgcaca tcaagcaatt
    28861 aataatgccg atactgtaac aggtatagcg aatattatta atgatcaaat cgacttgaat
    28921 aacgctatgg aaacattgaa acacttagtt gacaatgaaa ttccaactgc agaacaaact
    28981 atcaattacc aaaacgctga cgacgttgct aaatcaatct ttgatgatgc aaaacgtctg
    29041 gcaaatacat tgctaaatag tgataacaca aatgtgaatg atattaacgg cgcaattcaa
    29101 gcagtcaatg atgcaatcca aaatcttaat ggtgatcaac gtctacaaga agctaaggac
    29161 aaagcaattc aaaatgttaa caaagtctta gcagataaat tgaaagaaat agaagcttca
    29221 aatgcgacgg atcaagacaa gcttattgcg aaaaataaag cagaagaatt ggcaaacagt
    29281 atcattaaca acattaataa agcaacaagt aatcaggatg tatctcaagt tcaaacagct
    29341 ggcaaccatg cgattgaaca agtgcatgcc aatgaaatac cgaaagctaa aattgatgca
    29401 aataaagatg ctgataagca agtacaagca ttaattgacg aaattgatcg aaatccaaat
    29461 ctaacagata aggaaaaaca agcgcttaaa gatcgaataa atcaaatatt gcaacaaggt
    29521 cataacggca tcaacaatgc gatgactaaa gaagaaattg aacaagccaa agcacaactt
    29581 gcacaagcgt tacaagaaat taaagattta gtgaaagcta aagaaaatgc gaaacaagat
    29641 gttgataagc aagtacaagc attaattgac gaaattgatc gaaatccaaa tctaacagat
    29701 aaggaaaaac aagcgcttaa agatcgaatt aatcaaatat tgcaacaagg tcataatggc
    29761 attaacaatg cgatgactaa agaagaaatt gaacaagcca aagcacaact tgcacaagca
    29821 ttacaagaaa ttaaagattt agtgaaagct aaagaaaatg cgaaaaatgc aattaaagca
    29881 ttagcaaatg cgaagcgtga tcaaatcaat tcaaaccctg atttaacacc tgagcaaaaa
    29941 gcaaaagcgc tcaaagaaat tgacgaagct gaaaaacgag cactagaaaa tattgaaaat
    30001 gctcaaacta aagatcaatt aaatcaagga ttaaatttag gattagatga tattagaaat
    30061 actcatgtat gggaagttga tacacaacct gctgtaaatg aaatttttga cgcaactcct
    30121 gaacaaatcc ttgtaaatgg tgaactcatt gtacatcgtg atgacatcat tacagaacaa
    30181 gatattctag cacatataaa cttagttgat cagcttacag cagaagttat tgatacaccg
    30241 tcaaccgcaa caatttctga tagcttaaca gcaaaagtcg aagtaacatt acttgatgga
    30301 tcaaaagtaa ttgttaatgt tcctgtgaaa gtagtagaaa aagaattggc agtagtcaaa
    30361 caacaggcaa ttgaatcaat tgaaaatgcg gcacaacaaa agattaatga tatcaataat
    30421 cacgcaacat taacaccaga acaaaaagaa gctgcgattg cggaagttaa taagcttaaa
    30481 caacaagcta ttgagcaaat taacaatgca acagatgttc attcggtgga agaagttcaa
    30541 caacaagaac aaacgcatat tgaacaattt aatccagagc aatttacaat tgaccaagcc
    30601 aaatcaaatg ccattaaatc aattactgat gctattcaac atatgattga tgaaattaat
    30661 gctagtaagg atttaactga taaagagaaa caagaagcta tatctaagtt aaatcaatta
    30721 aaagaacagg caattcaagc gattcaacgt gcgcaaagta tcgatgaaat cacacaacaa
    30781 ttagagcaat tcaaagcgca aataaaagca gcaaatccat ttgctaaaga attggaaaaa
    30841 cgtaaaaaag tagcgatttc aaaaattaaa gatatttcga cagatgcaat tgaacgcatt
    30901 aaaaatagtt caactggaac tgctgaagaa agacaagcag caatgaatcg aattaacgaa
    30961 attgtacttg aaacaatcag agatattaat aatgcgcaaa cgccacaaca agttgaggct
    31021 gcattgaata atggtatcgc acgaattttg gcagtacaaa ttgttacatc taataatagc
    31081 aagcattcat cagattcgga cggtgaatct aatagtcata taagcattgg atatggaact
    31141 gtaaaccatc cattcaatag ttcggctatt ggacataaaa agaaattgga tcaagatgat
    31201 gaaatcgatc cacttcatat gcgtcatttc ggtgaccgaa ttggtaatgt tatcaaaaac
    31261 gcacttggcg ttgttggtat ttccggatta cttgctagct tctggttctt cattgcgaaa
    31321 cgtcgtcgta aagaggatga agaggaagaa ttggaaatta gagataatag caaagacaag
    31381 aagaaaggtt caatagaagg tactaaacat ttaccacttt tattagcgaa acgtcgcaga
    31441 aaagaagatg aagaagatgc tattgttgaa gaaaaagatt cgctaaacaa taacgagtca
    31501 ctcgataaag ttaaacatac gccgttcttc ttacctaaac gtcgtcgcaa agaagatgaa
    31561 gaagatgtgg aagttacaaa tgaaaacaca gatgaaaaag tgttgcaaga taacgaacat
    31621 tcaccactct taatcgcaaa acgacgcaaa gataaagagg tagatgttga aacaacacct
    31681 agtattgaat ctaatgaaga tgacgcacct ttattattgg ctaaaaagaa aaatcaaaaa
    31741 gataaccaat ccaaagggaa aaagtcggca tcaaaaaaac cttctaaaaa gctagtagct
    31801 aaaaagaaga aaaagaaatc taaaaaaaat aaaaaataat ttgtttcttc gataaaaaga
    31861 ggagcaccga ttgaaattat gtcagtcggt gctcctttta tttgttcttt ttaattaatt
    31921 tgtacaatgc ttgctgtgcg ttttgattcg cttctttgtt ttgttctctc ggaatccatt
    31981 taacaaataa taaatcaaaa tcttgttcaa atatttccaa ttgatcaaaa taaggtttaa
    32041 attttgcatt ttttacataa ccagcgttaa cactatccgc aattaactta gagtcagtgt
    32101 acaacaacgc attgctaacg tttaattcac gggcgtgttt caatgcatag atacaagcag
    32161 cccattctgc agtgtgatta tccatttcgc ctaactcatg tgtatatgta taatgctgat
    32221 catcttcttt gattacaacg gcacacgtac ttatgcctgg atttcctttc gtcgcagcat
    32281 caaaatttat tttcgccata ataaacctac tttctattca atacttagtt aaagttacta
    32341 ttactgtaat acaaaatata ttaaacaatc tattaaaaaa cacgcatcac ttaaataagt
    32401 aacacgtgtt taaaatactt gctgattcaa agatgatttt ctaatacgta gtactgtaat
    32461 atacttccta aaaaaatcat cttcaggctg ggacataaat caatgttcta tgctctacga
    32521 agttatattg gcagtagttg actgaacgaa aatgcgcttg taacaagctt ttttcaattc
    32581 tagtcaacct tgccggggtg ggacgacgaa ataaattttg cgaaaatatc atttctgtcc
    32641 cactccctta aaacttattc tattatgtag taagtgcgtt aataaccttg atctaattta
    32701 tcaatcttgc ctttacgata aaatgattta gcaatatatc caaatggtac attgaaaact
    32761 gttgaagcta attttagtac gtacgttgta ataaatattt caaatacaac tgtaccaggt
    32821 aaacttccga taaatgcgat agctacaaat aaagctgtat caattattga gcttaaaaat
    32881 gtactaccat atgcacggat gaaaaacgtt ttatcagaac taaatacttt tttaattagt
    32941 gaaaagataa atacatcaat atgttgacca ataatatatg cgacgattga gcctaaagca
    33001 atgcgtggca caacatcaaa gattgcgtgt aaagcttttt gtgccatatc ttctggtgca
    33061 ggaataaaat gtaatgacaa ttgcataaca ataatcataa ttaatgttga tgaaaagcct
    33121 aaccaaactg ctctttttgc aactctacgc ccataaatat cgtttaatat atctgttgct
    33181 aaataaatag aagcaaacat gacattacct aaagttgctg aaataccaaa gatttctaca
    33241 gttttaatta cttgtatgtt ggcaatgatt gtgccaattg caacccatgc aattaaacct
    33301 tgtttaccaa aaaagcgata cataagtacc ataagcacga acgttgcaat aaacgtaact
    33361 agtcctaaaa tttcattata catattaaaa tgtcctccta aattttgatc atgcgggtgt
    33421 ttagaaaccg ctcaataaat aacttactca ttttactgaa aaatcgtttg cgatgcaata
    33481 gcatttgaat ataaatacat tttacgtata gaattgtaaa aggtttcatt caaatcttag
    33541 ggtcaaaaat gttataatat ttttatgtca aatttaaagc agtaacactt atttacaagg
    33601 ttgcaatatt ttgaagtaat aaaggaagtg tcgcgtattt taactttttc agagcaaaat
    33661 gcactcgcga aaatagatga tttaatgaat acttattgca atcaatgtcc aatcaaaact
    33721 cgtctgcgta aattagaggg gaaaacgaag tcgcatcatt tttgtatcaa tgagtgttca
    33781 atagggaaag aaataaaaca attaggaaat gaacttcaat aggaggaagt aaaatgcaaa
    33841 ttatatctat atcagaaaca ccgaaccaca acacaatgaa gattacactt agtgaaagca
    33901 gagaaggtat gacatcagat acgtatacta aagttgatga ttcacagcca gcatttatta
    33961 atgacatctt aaaggttgaa ggcgttaaat caattttcca tgttatggac tttatttcag
    34021 tagataaaga aaatgacgca aattgggaaa cagtattgcc aaaagtagag gctgtattcg
    34081 aataaatttt tcatcaacta gtattcgggg ggaataaagt atatggaaat tttacgtata
    34141 gagccaacgc caagtccaaa tacaatgaaa gttgttttgt catatacaag agaagacaag
    34201 ttatctaata cttataaaaa agtagaagaa aatcaaccaa gatttataaa tcagttgtta
    34261 tctatagatg gtatcacttc catttttcat gtcatgaact tcttagctgt tgataaggca
    34321 ccaaaagctg attgggaagt catattacct gatattaaag ctgctttttc tggtgaaagt
    34381 caggtattgg aatctggaaa agacccacaa attgataaac attttggtga aattaaagct
    34441 gaattattaa cttttaaggg tataccgtat caaattaagc taacttctgc tgaccaggaa
    34501 ttaagagaac aattaccaca aacatacgtt gatcatatga ctcaagcgca aacagaacat
    34561 gacaatattg tttttatgcg taaatggcta gatttaggaa atcgctatgg aaatattgaa
    34621 gaagtaatgg atggtgtcct agaagaagtg ttagctacct atccagaatc acagttgccc
    34681 gtattggtaa aacatgcttt agaagaaaat cacgcagcta ataattatca tttctatcga
    34741 catgtctctt tggatgaata tcatgcgact gataattgga agactcgatt gcgaatgtta
    34801 aaccattttc caaagccgac ttttgaagat ataccgctgc ttgatttagc tttatctgat
    34861 gaaaaagtac cggttagacg tcaagcgatt gtattattag gcatgattga aagtaaagaa
    34921 attttaccgt atttatataa gggacttcgc gataaaagtc ctgctgtaag aaggacagca
    34981 ggggattgca taagtgattt agggtatcca gaggcactac cagaaatggt gctactttta
    35041 gatgattcac agaagatcgt taggtggcgt gctgctatgt ttatctttga tgaaggtaat
    35101 gcagagcagc ttcccgcact taaagcccat attaatgaca atgcgtttga agttaaatta
    35161 caaattgaaa tggccatatc tcgtattgaa aacggagatg aagcacttgg ttcagtatgg
    35221 aaacaaatgg caaacagaac aatttaattg gaggaattaa atatgaatgc atatgatgct
    35281 tatatgaaag aaattgcgca acaaatgcgt ggcgaattaa ctcaaaatgg ttttacaagt
    35341 ttagaaacga gcgaagcagt attagagtat atgaaccaag taaatgctga tgacacaact
    35401 tttgtagtta ttaactctac atgcggctgt gcagctggat tagcaagacc agctgcagta
    35461 gctgttgcaa cacaaaatga acatagacca acaaatacag ttacagtttt tgcagggcaa
    35521 gataaagaag caactgctac tatgcgagaa ttcattcagc aggtaccatc tagtccttcg
    35581 tatgctttat tcaaaggtca agatttagtt tattttatgc ctagagaatt tatcgaaggt
    35641 agagatatta atgacattgc aatggactta aaggatgcct ttgacgaaaa ttgtaaatag
    35701 tacacataaa taaatataaa ggttaacaca ttctataata ttaaaaatgg tgtctgtcat
    35761 tgaaaataga gaatttagtt gtattctatt tgttatataa agtccgtaca cttcaactat
    35821 attttctata aatttaactg ttttaatagg agatcaaaca taatattcaa atcatgtgtt
    35881 aacctctttt ttaaaatttt ttagcattaa agttatagat ttgggtaaac aattagcaat
    35941 tggaaacatt tatcacttta cgatgtggta ggtacttaat cagcatttta taaattaagt
    36001 aacggaatcc atgatattaa tatcacattc ctaaaatgag tgataacaaa atgctacata
    36061 aagttaaatt atatcaaact aaatatacat actataaata atgaaaatga ggtgttatcg
    36121 catatgttga attcatttga tgcagcatat cacagtcttt gtgaagaagt tttagaaata
    36181 ggaaatacac gaaatgatcg cacaaataca ggtacgattt cgaaatttgg tcatcaactt
    36241 cgctttgact tatctaaagg atttccacta ttaacgacaa agaaagtttc ttttaaatta
    36301 gtagcaaccg aattattatg gttcattaaa ggagatacaa acatccaata cttattaaaa
    36361 tataataata atatttggaa cgaatgggct tttgaaaatt atattaaatc agacgagtat
    36421 aaaggtccag atatgacaga tttcggacat cgtgcattga gtgatcctga atttaacgaa
    36481 caatataaag aacaaatgaa acaatttaag caacgtattc ttgaagatga tacatttgcg
    36541 aagcaattcg gtgatttagg aaatgtttat ggtaaacaat ggcgagattg ggttgataaa
    36601 gatggtaatc attttgatca acttaaaaca gtaattgaac aaattaagca taatccagat
    36661 tcaagacgac acatcgtatc tgcatggaat ccaacagaaa ttgatacaat ggcacttccg
    36721 ccttgtcata caatgttcca gttttatgtc caagatggta agttaagttg ccagttatac
    36781 caacgtagcg cagatatctt tttaggtgtg ccatataata tcgcaagcta cgctttattg
    36841 acacacctta ttgccaaaga atgtggactt gaagtaggtg aatttgtgca tacatttgga
    36901 gatgcacata tttattcaaa tcatattgat gcaattcaaa ctcaattagc gcgtgaaagt
    36961 ttcaatcctc caacattaaa aattaacagt gacaagtcta ttttcgacat aaattatgaa
    37021 gatttggaaa ttgttgacta tgaatcgcat ccagcaataa aagctccaat agcagtgtag
    37081 tcattgcata gttagctaac catatagaca tcaaaatgac atcatagtat tttcaagtgc
    37141 aaaaaagtac ttttttgtgt taaacgtttt cataaattat gcaaaatcat tatttctatc
    37201 acactttatg ataaaaattg tgttaaatta aagataactt agtaataaaa aatgaaatga
    37261 tagaagaagg aggataatta tgactttatc cattctagtt gcacatgact tgcaacgagt
    37321 aattggtttt gaaaatcaat taccttggca cctaccaaat gatttgaagc atgttaaaaa
    37381 actatcaaca ggtcatactt tagtaatggg tcgtaagaca tttgaatcga ttggtaaacc
    37441 actaccgaat cgtcgaaatg ttgtacttac ttcagataca agtttcaacg tagagggcgt
    37501 tgatgtgatt cattctattg aagatattta ccaactaccg ggacatgttt tcatatttgg
    37561 agggcaaaca ttatttgaag aaatgattga taaagtggac gacatgtata ttactgttat
    37621 tgaaggtaaa ttccgtggtg atacgttctt cccaccttat acatttgaag actgggaagt
    37681 tacctcttca gttgaaggta aactagatga gaaaaataca attccacata cctttctaca
    37741 tttaattcgt aaaaaataag ggggaaaacg accatgacaa aacagattat agtaacagac
    37801 tcaacatccg atttatctaa agaatattta gaagcaaaca acattcatgt aattccttta
    37861 agtttaacta ttgaaggagt ttcatacgtt gaccaagtag atattacatc agaagaattt
    37921 attaatcata ttgaaaatga tgaagatgta aagacaagtc agccagccat aggtgaattt
    37981 atatctgctt atgaagaact aggaaaagat ggctccgaaa tcataagtat tcatctttct
    38041 tcaggattaa gtggtacata taacactgct taccaagcaa gtcaaatggt agatgctaat
    38101 gtaactgtta ttgattcaaa atctatttct tttggtttag ggtatcaaat acaacaccta
    38161 gtagagcttg taaaagaagg tgtctcaact tctgaaatag ttaaaaagtt aaatcattta
    38221 agagaaaaca ttaaattatt tgtagttata gggcaattga atcaattaat taaaggtggc
    38281 agaattagta aaacaaaagg tttgattggt aatcttatga aaatcaaacc aattggtaca
    38341 ctagatgatg gtcgcttaga gcttgtgcac aatgcgagaa ctcaaaattc tagtatccaa
    38401 tacttgaaaa aggaaattgc tgaatttata ggagatcatg aaatcaaatc cgttggtgtc
    38461 acacatgcta acgttattga atatgttgat aaattgaaga aagtttttaa tgaagctttt
    38521 catgtgaata attacgatat aaatgtaact acaccagtta tttctgcaca tactggtcaa
    38581 ggtgcgattg gcctcgtagt ccttaagaag taaatttaat cttttcagtg ttaattactt
    38641 ccatttcaat cctttataga ctaaatttat aattagatag atagaggagg taattcatat
    38701 gacaaaagaa tatgcaacat tagcaggagg atgtttctgg tgcatggtta aaccatttac
    38761 atcatatcca ggcatcaagt cagtcgtatc tggttatagt ggcggtcatg ttgacaaccc
    38821 aacttatgaa caggtatgta cgaatcaaac cggccatgtc gaagcagtac aaattacgtt
    38881 tgatccagag gttacttcct ttgaaaatat attagacata tatttcaaaa catttgaccc
    38941 aaccgatgat caagggcaat ttttcgatag aggcgaaagc tatcaaccag tcattttcta
    39001 tcatgatgag catcagaaaa aggctgctga gtttaaaaag caacaattaa atgaacaagg
    39061 tattttcaag aaaccagtga ttacacctat taaaccatat aaaaatttct atccagctga
    39121 agactaccat caagattatt acaaaaagaa cccggtacat tattaccaat atcaacgtgg
    39181 ttcaggtaga aaagcgttta tagaatcaca ttgggggaat caaaatgctt aaaaaagata
    39241 aaagcgaact aacagatata gaatatattg ttacacaaga aaatggtact gaaccaccat
    39301 ttatgaatga atattggaat cattttgcta aagggattta cgtagataag atttctggta
    39361 aacctttatt tacatctgaa gaaaagtttc attctgagtg cggatggcct agcttttcca
    39421 aagcgcttga tgacgatgaa attatagaat tagtcgacaa atcatttggt atggtaagaa
    39481 ctgaagtccg ttcagaagaa tcaaatagtc atttaggaca cgtctttaat gatggaccta
    39541 aagaaagtgg cggcttaaga tactgtatca attccgctgc aattcaattt attccatatg
    39601 aaaaattaga agaattgggt tatggcgatt taatatcaca ttttgataag taggagtgga
    39661 aagtatgttt aaaaaattat tcggaaaagg caaagaagtt cagaaagata tcgcaattta
    39721 tgcaccatta actggagaat acgtgaaaat tgaagatatt ccagatcctg tattcgcaca
    39781 aaaaatgatg ggcgaaggtt ttggtattaa tccaactgaa ggagaagttg tgtctccaat
    39841 tgcaggacgt gttgacaatg tctttccaac taagcatgcg attgggttaa aagcagataa
    39901 cggattagaa ttattagttc atatcggttt agacacagtt caattagacg gtgaaggatt
    39961 tgaagtgtta gtgtctagtg gtgacgaagt taacgtcgga gatccattag taagattcaa
    40021 ccttgaattt attaataata acgctaaatc tgttatttca ccaattataa ttacaaacac
    40081 tgatcaagca gcttcaatta atatttatga tgaaaatgct gtgattaaag gtgaaacaaa
    40141 agtgattgat gtgacaatga actaatgaaa aactattcgt tttatcaatt tgtcatgaca
    40201 gttcgtggtc gacacgacga taaaggtcgt ctagcagaag agatatttga cgatcttgct
    40261 ttcccaaaac acgatgatga ttttaacata ctgtctgatt atattgagac acatggtgat
    40321 ttcacattgc caatgtctgt atttgatgat ttatatgaag aatatacgga atggctaaaa
    40381 ttttaacata atatttaata atagttagaa ccagggtgat gcaatttgtt atcctggttt
    40441 taatttaaaa taaactaagt tgtgactaaa aattaatcaa ttatagtgaa atatggtgcg
    40501 ctatcttgca taaattgata tgattaacta cacagaattt aaaagtacat aatacataat
    40561 aaggaagtga tacaatggat gataagcaac acacatcttc atccgatgat gaacgcgctg
    40621 aaattgcaac aagcaatcaa gaccaacaaa ctaattcatc gaaacgcgtt cacttaaaac
    40681 gttggcaatt catatcaatt ttaattggta caatcctaat aacagctgtc atcacagttg
    40741 ttgcatatat ttttataaat caaaaaataa gtggtttaaa caaaactgat caagcaaact
    40801 taaataaaat tgaaaatgtg tataaaatct taaatagtga ttattacaaa aaacaggact
    40861 ctgacaagtt aagtaaagct gcaattgatg gcatggtcaa agaattaaaa gatccttatt
    40921 ctgaatattt aacaaaagaa caaacgaaat cctttaatga aggtgtttca ggtgattttg
    40981 taggtattgg tgcagaaatg caaaagaaaa atgatcaaat tatggttact agtcctatga
    41041 agggatctcc agcagaacgt gctggcattc gtcctaaaga tgtcattact aaagtaaatg
    41101 gaaaatcaat taaaggtaaa gcattagatg aagttgtcaa agatgttcgt ggtaaagaaa
    41161 acactgaagt aactttaact gttcaacgag gcagtgaaga aaaagacgtt aagattaaac
    41221 gtgaaaaaat tcatgttaaa agtgttgagt ataagaaaaa agataaggtt ggagttatta
    41281 ctattaataa attccagaat gatacatcag gtgaattgaa agatgcagtt cttaaagctc
    41341 acaaagatgg tttgaaaaag attgttttag atttaagaaa taatcctggt ggactactag
    41401 atgaagctgt taaaatggca aatattttta tcgataaagg aaaaactgtt gttaaactag
    41461 aaaaaggtaa agatactgaa gcaattcaaa cttctaatga ctcgttaaaa gaagcgaaag
    41521 acatggatat atccatctta gtgaatgaag gttcagctag cgcttctgaa gtatttactg
    41581 gtgcgctaaa ggactataat aaagctaaag tttatgggtc aaaaacattc ggtaaaggtg
    41641 tcgtacaaac tacaagagag tttaaggacg gttcattgtt aaaatatact gaaatgaaat
    41701 ggttaacgcc agatggtcat tatattcacg gtaaaggcat taaaccagac gttactattg
    41761 acacacctaa atatcaatct ttaaatgtca ttcctaatac gaaaacattt aaagttggag
    41821 acgatgataa aaatattaaa actattaaaa ttggtttatc agctttaggt tataaagttg
    41881 ataatgaatc aacgcaattt gatcaagctt tagaaaatca agttaaagct ttccaacaaa
    41941 cgaataaact cgaggtaact ggtgaattta ataaagaaac gaataataaa tttactgagt
    42001 tattagttga aaaagctaat aaacatgatg atgttctcga taaattgatt aatattttaa
    42061 aataagtgat acacactgct aaaattgttt tattatgtta atgacacgcc tcctaaattt
    42121 gcaaagatag caatttagga ggcgtgttta tttttattga cgtctaactc taaaagatat
    42181 aaattagaca tttacaaatg atgtaaataa cgcaatttct atcatcgctg ttaacaattc
    42241 atgctttaat atagtatgag catatacttt ttaaatagta ttattcacta gttttaacaa
    42301 tcgattaatt ggtatatgat acttttattg gttattttta tcccatagtg tgataattac
    42361 tatttttcat tcataataaa ggtttaaagc atgttaatag tgtgtaagat taacatgtac
    42421 tgaaaaacat gtttaacata acgatataag gagtgacgta catgatccgt ctaggtaaaa
    42481 tgtcagattt agatcaaatc ttaaatctag tagaagaagc aaaagaatta atgaaagaac
    42541 acgacaacga gcaatgggac gatcagtacc cacttttaga acattttgaa gaagatattg
    42601 ctaaagatta tttgtacgta ttagaggaaa ataacaaaat atatggtttt attgttgtcg
    42661 accaagacca agcagaatgg tatgatgaca ttgactggcc agtaaataga gaaggcgcct
    42721 ttgttattca tcgattaact ggttcgaaag aatataaagg agctgctaca gaattattca
    42781 attatgttat tgatgtagtt aaagcacgtg gtgcagaagt tattttaacg gacacctttg
    42841 cgttaaacaa acctgcacaa ggtttatttg ccaaatttgg atttcataag gtcggtgaac
    42901 aattaatgga atatccgcca tatgataaag gtgaaccatt ttatgcatat tataaaaatt
    42961 taaaagaata gaggtaatat taatgaagaa aatcgcattt accggagggg gaacagttgg
    43021 acacgtatca gtaaatttaa gtttaattcc aactgcatta tcacaaggtt atgaagcgct
    43081 ttatattggt tctaaaaatg gtattgaaag agaaatgatt gaatcacaac taccagaaat
    43141 taagtattat cctatttcaa gtggtaaatt aagaagatat atttctttag aaaatgccaa
    43201 agacgtattt aaagtattga aaggtattct tgatgctcgt aaagttttga aaaaagaaaa
    43261 acctgatcta ttattttcaa aaggtggatt tgtatctgtg ccagttgtta ttgcagccaa
    43321 atcattaaat ataccaacta ttattcatga atctgactta acaccaggat tagcgaataa
    43381 gatagcactt aaatttgcca agaaaatata tacaacattt gaagaaacgc taaactacct
    43441 acctaaagag aaagctgatt ttattggagc aacaattcga gaagatttaa aaaatggtaa
    43501 tgcacataat ggttatcaat taacaggctt taatgaaaat aaaaaagtgt tactcgttat
    43561 gggtggaagc ttaggaagta aaaaattaaa tagcattatt cgcgaaaact tagatgcatt
    43621 attacaacaa tatcaagtga tacatttaac tggtaaagga ttaaaagatg ctcaagttaa
    43681 aaaatcagga tatatacaat atgaatttgt taaagaggaa ttaacagatt tattagcaat
    43741 tacggacaca gtaataagta gagctggatc aaatgcgatt tatgagttct taacattacg
    43801 tataccaatg ttattagtac cattaggttt agatcaatcc cgaggcgacc aaattgacaa
    43861 tgcaaatcat tttgctgata aaggttatgc taaagcgatt gatgaagaac aattaacagc
    43921 acaaatttta ttacaagaac taaatgaaat ggaacaggaa agaactcgaa ttatcaataa
    43981 tatgaaatcg tatgaacaaa gttatacgaa agaagcttta tttgataaga tgattaaaga
    44041 cgcattgaat taatgggggg taatgcttta tgagtcaatg gaaacgtatc tctttgctca
    44101 tcgtttttac attggttttt ggaattatcg cgtttttcca cgaatcaaga cttgggaaat
    44161 ggattgataa tgaagtttat gagtttatat attcatcaga gagctttatt acgacatcta
    44221 tcatgcttgg agctactaaa gtaggtgaag tctgggcaat gttatgtatt tcattacttc
    44281 ttgtggcata tctcatgtta aagcgccaca aaattgaagc attatttttt gcattaacaa
    44341 tggcactatc tggaattttg aatccagtac taaaaaatat attcgataga gaaagaccta
    44401 cattgctgcg tttaattgat ataacaggat ttagttttcc tagcggtcat gctatgggat
    44461 caactgcata ttttggaagt ggtatctatc tattaaaacg attaaatcaa ggtaattcaa
    44521 aaggtattct tatagggtta tgtgcagcta tgattttatt gatttccata tcacgtgtat
    44581 atctaggtgt gcattatcca acagatatta ttgccggtat tattggtggc gtattttgca
    44641 ttattttatc aacgttatta cttagaaata aattaatcaa ttaaatagta aaaaaacaaa
    44701 agcagtaaac ctaaagtgtc gtaagggttt actgctttta taaaacgttg ttataacgta
    44761 tattgtcttt tacgggcata taaaagggga atatttgaaa atgaccaatc caacaagaac
    44821 gaaacgttgt gggggggatg ttctatgtgg tattgataat cat
//
LOCUS       DAHHOJ010000005        44344 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.005, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000005 DAHHOJ010000000
VERSION     DAHHOJ010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 44344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44344
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.005"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..983)
                     /locus_tag="NBH32_000201"
     CDS             complement(<1..983)
                     /locus_tag="NBH32_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCD1513841.1"
                     /translation="MKAIQIKKYNKNRDVQIRDITIPEIKSTEVLVKVAYAAINPLEN
                     LTINGSVKLIQNYKMPQTIGNELTGTIVKVGSKVEDYKLGDKIYSRLPIDRIGAFAEY
                     VSIESNAIAKLPKNLNLKTGAAVPLTGLTAYQAITEELKAIPGKTLFIPGGSGSFGQM
                     AVPIAKSLGLKVIVSGSPRLKDQFLAKGVDQYIDYKTENYCEVLSDIDYVIDTLGPNE
                     FDKELSVMKPGGTIVSLINAPNKAFAVKNNFSPFKKLLFGLAGKKFDKKAQKKSIDYR
                     FIFVRSDGEQLKILNKIIEQNNIIPDIDDHIFTIDEIDKALDYTFNHHTNG"
     gene            complement(1124..1954)
                     /locus_tag="NBH32_000202"
     CDS             complement(1124..1954)
                     /locus_tag="NBH32_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000448903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1513842.1"
                     /translation="METLELQGAKLRYHQVGQGPVLIFIPGANGTGDIFLPLAEQLKD
                     HFTVVAVDRRDYGESELTEPLPDSASNPDSDYRVKRDAQDIAELAKSLSDEPVYILGS
                     SSGSIVAMHVLKDYPEVVKKIAFHEPPINTFLPDSTYWKDKNDDIVHQILTEGLEKGM
                     KTFGETLNIAPIDAKMMSQPADTEEGRIEQYKRTMFWSEFEIRQYTHSDITLDDFTKY
                     NDKITLLNGTDSRGSFPQDVNFYINNETGIPIVDIPGGHLGYIQKPEGFADVLLNMWS
                     "
     gene            complement(1974..2828)
                     /locus_tag="NBH32_000203"
                     /pseudo
     CDS             complement(1974..2828)
                     /locus_tag="NBH32_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
     gene            complement(2924..4129)
                     /locus_tag="NBH32_000204"
     CDS             complement(2924..4129)
                     /locus_tag="NBH32_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1513843.1"
                     /translation="MWIEKFKNKNNETKYRYYEKYKDPYTDKWKRVSVVLNKNTKQSQ
                     KEAMFRLEEKIKEKLNNKSSSELKTLTFHALLDEWLEYHIKTSGSKLTTLNNIKIRIR
                     NIKRYSSENLLLNKLDTKYMQIFINKLSDIYSQNQVTRQLGDMKGAIKYAVKFYNYPN
                     EYLLTNVKIPKRRKTIEDIEKDESKMYNYLEMNQVLQIRDHILNDNKLHKRNRILIAS
                     ILEVQALTGMRIGELQALQEKDIDLLNKTINITGTIHRIKYEEGFGYKDTTKTISSKR
                     SISINSRTVEIFKKIILENKMLKRWNSSYVDRGFIFTTKKGNPLCNNQIAGVLKKTTK
                     ALNMNKKVTTHTFRHTHITLLVEMNVSLKAIMKRVGHVDEKTTIRIYTHVTEKMDREL
                     TQKLENIPS"
     gene            4255..4869
                     /locus_tag="NBH32_000205"
     CDS             4255..4869
                     /locus_tag="NBH32_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCD1513844.1"
                     /translation="MTQFLGALLLTGVLGYIPYKYLTMIGLVSEKNKVINTPVLLIFS
                     IETCLIWFYSFIIFNNVDLKNLNLIQLLTGLKANILFLFIFVLTVFVFNPLIVKFIIW
                     LINITRKFMKLDCISLLDKRDKLFNNNGKPVFIVIKDFENRIIEEGELKTYNSAGSDF
                     DLLEVERQDFKVSDLPSNDELYIKHTLVDLKQQIKLDLYLMNEY"
     gene            complement(4866..5012)
                     /locus_tag="NBH32_000206"
     CDS             complement(4866..5012)
                     /locus_tag="NBH32_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513845.1"
                     /translation="MNGGENFMVDKNKKQETTRSNPLNKSFEKSGASEKLKSTLSEKA
                     KKKD"
     gene            complement(5049..5231)
                     /locus_tag="NBH32_000207"
     CDS             complement(5049..5231)
                     /locus_tag="NBH32_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513846.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNARKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(5302..6072)
                     /locus_tag="NBH32_000208"
     CDS             complement(5302..6072)
                     /locus_tag="NBH32_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1513847.1"
                     /translation="MREKVSNRLKHIMKIRNLKQVDIINKSKPYQKQLGISLSKSTLS
                     QYINDVQSPDQDRIYLLSKTLNVGEAWLMGYDVNSYRVPDEERQEETVMSKINNISSQ
                     LTPPRQSNVLNYANSQLDEQNKVTSIDGYKESKLVSYIACGATGAGIGEELYDDILHE
                     EVFFKEDETPSNADFCILVNGDSMEPMLKQGTYAFIKKEDSIKDGTIALVVLDGVSLI
                     KRVDICEDYINLVSLNPKYDDIKVASFSNIKVMGKVVL"
     gene            6232..6453
                     /locus_tag="NBH32_000209"
     CDS             6232..6453
                     /locus_tag="NBH32_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HCD1513848.1"
                     /translation="MCYDYSRLSGKIVEKYGTQYNFAIAMKLSERSLSLKLNGKVGWK
                     DSEIWKAIQLLGIPVEKIHLYFFKEKVHV"
     gene            6477..6920
                     /locus_tag="NBH32_000210"
     CDS             6477..6920
                     /locus_tag="NBH32_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513849.1"
                     /translation="MEQITLTKEELKEIIAKEVREAINGKKPISSGAIFSKVRINNDD
                     LEEINKKLNFAKDLSLGRLRKLNHPIPLKKYQHGFESIHQKAYVQDVHDHIRKLTLSI
                     FGVTLNSDLSESEYNLAAKIYRDIKNYYLYIYEKRVSELTIDDFE"
     gene            6935..7084
                     /locus_tag="NBH32_000211"
     CDS             6935..7084
                     /locus_tag="NBH32_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000771849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513850.1"
                     /translation="MKLLRRLFNKKHENLIDVWHGNQWLKVKESKLKKYKVVSDREGK
                     KYLIK"
     gene            complement(7125..7337)
                     /locus_tag="NBH32_000212"
     CDS             complement(7125..7337)
                     /locus_tag="NBH32_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000362644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD1513851.1"
                     /translation="MDLEKIAHDITISLLPRALDRHKIHNEWQEVGDDVIAFAKDSVA
                     RDYFSIYSSVLLGLQEEEKSRKDLGL"
     gene            7407..7604
                     /locus_tag="NBH32_000213"
     CDS             7407..7604
                     /locus_tag="NBH32_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513852.1"
                     /translation="MQAQNKKVIYYYYDEEGNRRPLDIQINDGYELMVRSHFINNTIE
                     EIPYVNNNLYALVDGYEFKLD"
     gene            complement(7591..7971)
                     /locus_tag="NBH32_000214"
     CDS             complement(7591..7971)
                     /locus_tag="NBH32_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2513 domain-containing protein"
                     /protein_id="HCD1513853.1"
                     /translation="MKLNHDCVRLLLLEIESNKKIGEPLTRHNFNDNIIFDKYDFETV
                     MYSLLKLEEAKFICCDLKFIEGRVVSWIIDDITWSGHEFLDNIRDNKTWNEVKRVVNK
                     TSSMSLNLMGKLAFQYLSQKFNLT"
     gene            8038..8283
                     /locus_tag="NBH32_000215"
     CDS             8038..8283
                     /locus_tag="NBH32_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HCD1513854.1"
                     /translation="MNIQEATKIATKNLVSMTRKDWKESHRTKILPTNDSFLQCIISN
                     SDGTNLIRYWQPSADDLMANDWEVINPTRDQELLKQF"
     gene            complement(8252..8617)
                     /locus_tag="NBH32_000216"
     CDS             complement(8252..8617)
                     /locus_tag="NBH32_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513855.1"
                     /translation="MPLISDEFDTLTKDQQYILSVLYKDYLECVKLGSVKLTCNNFGS
                     AKDIHTKYFQKLHFEDVKYDLNKLKNSGFLNGVYASNTIYHVTISDKTVVYFENEFKN
                     NLKSIIDSISKIASIIPGL"
     gene            8672..8887
                     /locus_tag="NBH32_000217"
     CDS             8672..8887
                     /locus_tag="NBH32_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513856.1"
                     /translation="MNTRSEGLRIGVPQVSSKADASSSYLTEKERNLGAEILELIKKS
                     DYSYLEINKVLYALDRELQYRANNNKL"
     gene            8912..9175
                     /locus_tag="NBH32_000218"
     CDS             8912..9175
                     /locus_tag="NBH32_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1513857.1"
                     /translation="MPKIIIPPTPENTYRGEEKFVKKLYATPTQIHQLFGVCRSTVYN
                     WLKYYREDNLGVENLYIDYSATGTLINISKLEEYLIRKHKKWY"
     gene            9188..9349
                     /locus_tag="NBH32_000219"
     CDS             9188..9349
                     /locus_tag="NBH32_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCD1513858.1"
                     /translation="MSDTYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            9428..9751
                     /locus_tag="NBH32_000220"
     CDS             9428..9751
                     /locus_tag="NBH32_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrmB family transcriptional regulator"
                     /protein_id="HCD1513859.1"
                     /translation="MTLQQKILSHFATYDNFNSDDVVEVFGISKTHAKSTLSRLKKKG
                     KIELESWGIWRVVEPQLHLTVVERKKEILEEQFELLARLNEQSDDPREIEERIKLMIR
                     LANQF"
     gene            9766..10128
                     /locus_tag="NBH32_000221"
     CDS             9766..10128
                     /locus_tag="NBH32_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513860.1"
                     /translation="MAILEGIFEELKLLNKNLRVLNTELSTVDSSIVQEKVKEAPMPK
                     DETAQLESVEEVKETSADLTKDYVLSVGKEFLKKADTSDKKEFRNKLNELGADKLSTI
                     KEEHYEKIVDFMNARINA"
     gene            10125..11291
                     /locus_tag="NBH32_000222"
     CDS             10125..11291
                     /locus_tag="NBH32_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2800 domain-containing protein"
                     /protein_id="HCD1513861.1"
                     /translation="MKLDHSNRAHAKLSASGAKQWLNCPPSIKASEGIADKSSVFAEE
                     GTFAHELSELYFSLKYEGLTQFEFNKAFQNYKRNQYYSEELREYVEEYVANVEEKYNE
                     ALSRDDDVIALFETKLDLGKYVPESFGTGDVIIFSGGVLEIIDLKYGKGIEVSAIDNP
                     QLRLYGLGAYELLSLMYDIHTVRMTIIQPRIDNFSTEELPISRLLQWGTDFVKPLARL
                     AYNGEGEFKAGSHCRFCKIKHSCRTRAEYMQNVPQKPPHLLSDEEIAELLYKLPDIKK
                     WADEVEKYALDQAKENDKNYPGWKLVEGRSRRMITDTNATLEKLVEAGYKPEDITETK
                     LLSITNLEKLIGKKAFSKIAEGFIEKPQGKLTLATESDKRPAIKQSAEDDFDKL"
     gene            11317..11871
                     /locus_tag="NBH32_000223"
     CDS             11317..11871
                     /locus_tag="NBH32_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000647288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2815 family protein"
                     /protein_id="HCD1513862.1"
                     /translation="MKAQLKNETKVITGKVRASYAHIFEPHSMDESTPKKYSVSLIIP
                     KEDTQMVEIIEKAIENAKEAFKGKWNGKIPNNLKTPLRDGDIDREDDPNYENAYFINA
                     TSQNAPGVVSPAMVRLNEPGSVVSGDYIRAVINFYGYNVNGNKGIAAGLNNIQLVEKG
                     EPLGGASAAEDDFDELDTDDEDFL"
     gene            11940..13892
                     /locus_tag="NBH32_000224"
     CDS             11940..13892
                     /locus_tag="NBH32_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HCD1513863.1"
                     /translation="MNIDIETYSSNDISKCGVYKYTEAEDFEILIIAYSIDGGAISAI
                     DMTKVDTEPFHADYETFKIALFDPAVKKYAFNANFERTCLAKYFNKQMPPEEWICTMV
                     NSMRIGLPASLDKVGEVLRLQNQKDKAGKNLIRYFSIPCKPTKVNGGRTRNLPEHDLE
                     KWQQFIDYCIRDVEVEMTIANKIKDFPVTVIEQAYWVFDQHINDRGIKLSKSLMLGAN
                     VLDKQSKEELLKQAKHITGLENPNSPTQLLAWLKDEQGLDIPNLQKKTVQDYLKVATG
                     KAKKMLEIRLQMSKTSVKKYNKMHDMMCSDERVRGLFQFYGAGTGRWAGRGVQLQNLT
                     KHYISDTELEIARDLIKEQRFDDLDLLLNVHPQDLLSQLVRTTFTAEEGNELAVSDFS
                     AIEARVIAWYAKEQWRLDVFNTHGKIYEASASQMFNVPVESITKGDPLRQKGKVSELA
                     LGYQGGAGALKAMGALEMGIEENELQGLVDSWRNANPNIVNFWKACQEAAINTVKSRK
                     THHTHGLRFYMKKGFLMIELPSGRALAYPKALVGENSWGSQVVEFMGLDLNRKWSKLK
                     TYGGKLVENIVQATARDLLAISIARLEASGFKIVGHVHDEVIVEIPRGSNGLKEIETI
                     MNKPVDWAKGLNLNSDGFTSPFYMKD"
     gene            13905..14090
                     /locus_tag="NBH32_000225"
     CDS             13905..14090
                     /locus_tag="NBH32_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3113 family protein"
                     /protein_id="HCD1513864.1"
                     /translation="MQHQAYINASVDIRIPTEVESVNYNQIDKEKENLADYLFNNPGE
                     LLKYNVINIKVLDLEVE"
     gene            14090..14491
                     /locus_tag="NBH32_000226"
     CDS             14090..14491
                     /locus_tag="NBH32_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PVL family protein"
                     /protein_id="HCD1513865.1"
                     /translation="MARRKVIRVRIKGKLMTLREVSEKYHISPELLRYRYKHKMRGDE
                     LLCGRKDSKSKDEVEYMKSQIKDEEKGREKIRKKAILNRYQRNVRAEYEEERKRRLRP
                     WLYDGTPQKHSRDPYWFDVTYNQMFKKWSEA"
     gene            14491..14748
                     /locus_tag="NBH32_000227"
     CDS             14491..14748
                     /locus_tag="NBH32_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3310 domain-containing protein"
                     /protein_id="HCD1513866.1"
                     /translation="MSIISNRKVDMNETQDNVKQPAHYTYGDIEIIDFIEQVTAQYPP
                     QLAFAIGNAIKYLSRAPLKNGHEDLAKAKFYVDRVFDLWEG"
     gene            14751..14951
                     /locus_tag="NBH32_000228"
     CDS             14751..14951
                     /locus_tag="NBH32_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513867.1"
                     /translation="MATQKQVDYVMSLQEQLELEDCEKYTDEQVKAMSHKEVSNVIEN
                     YKTSIRNEELYYECMSFGLPNC"
     gene            14966..15208
                     /locus_tag="NBH32_000229"
     CDS             14966..15208
                     /locus_tag="NBH32_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513868.1"
                     /translation="MTDSARKERLNQFFGSKRYLYQDNERVAHIHVVNDIYYFHGHIV
                     PGWQGVKKTFDTAEELEIYIKQHGLEYEEQKQLTLF"
     gene            15223..15471
                     /locus_tag="NBH32_000230"
     CDS             15223..15471
                     /locus_tag="NBH32_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCD1513869.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GLPNAMQDALKEDIYLDEAVGIMVSQVVYKYEEEQEND"
     gene            15464..15991
                     /locus_tag="NBH32_000231"
     CDS             15464..15991
                     /locus_tag="NBH32_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCD1513870.1"
                     /translation="MTNTLQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDAQVYLTT
                     NEQCFDIQGEMENSFVNNAKKKPFTINDYYEIYKGDKLAQLVIVPIWTPELKQVEEFE
                     SVSERGAKGFGSSGV"
     gene            16028..16264
                     /locus_tag="NBH32_000232"
     CDS             16028..16264
                     /locus_tag="NBH32_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCD1513871.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     ESQNTPIVYYTNNSKVTLFERPSEEVLGSLFEKK"
     gene            16289..16525
                     /locus_tag="NBH32_000233"
     CDS             16289..16525
                     /locus_tag="NBH32_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000483480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513872.1"
                     /translation="MFNTPKMKLPEKHTEVFKTYKNGTPEEKAEIEGCFIKTVKDEDS
                     EFYSPMLASLNEQQLKSMLRQVLFLIDTGDDNDD"
     gene            16515..16901
                     /locus_tag="NBH32_000234"
     CDS             16515..16901
                     /locus_tag="NBH32_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513873.1"
                     /translation="MMIKKLKNMDWFDIFIAGILRLFGVIALMLVVISPIYTVASYQN
                     KEVHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLFKKKFDSADIQARLKVGDKV
                     KVKTIGYRIHFLNLYPVLYEVKKVGK"
     gene            16901..17053
                     /locus_tag="NBH32_000235"
     CDS             16901..17053
                     /locus_tag="NBH32_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000595263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1513874.1"
                     /translation="MVKQILRLLFLLAMYELGKYVTEQVYIMMTANNDVEAASDFEKI
                     RAEVSW"
     gene            17121..17321
                     /locus_tag="NBH32_000236"
     CDS             17121..17321
                     /locus_tag="NBH32_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCD1513875.1"
                     /translation="MWIVISIVLSIFLLILLSSISHKMKTIEALEYMNAYLFKQLVKN
                     NGVEGLEDYENEVERIRKRFKS"
     gene            17373..19820
                     /locus_tag="NBH32_000237"
     CDS             17373..19820
                     /locus_tag="NBH32_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000884880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence-associated E family protein"
                     /protein_id="HCD1513876.1"
                     /translation="MLDKVTQIETIKYDRDVSYSYAASRLSTHWTNHNMAWSDFMQKL
                     AQTVRTKEDLTEYNKMSKSEQADIKDVGGFVGGYLKEGKRRAGQVMNRSMLTLDIDYA
                     AQDMTDILSMFYDFAYCLYSTHKHREISPRLRLVIPLKRNVNADEYEAIGRKVADIVG
                     MDYFDDTTYQPHRLMYWPSTSNDAEFFFTYEDLPLLDPDKILNEYVDWTDTLEWPTSS
                     KEESKTKRLADKQGDPEEKPGIVGAFCRAYTIEEAISTFIPDLYEKHSTNRYTYHEGS
                     TAGGLVLYENNKFAYSHHNTDPVSGMLVNSFDLVRIHLYGAQDEDAKTDTPVNRLPSY
                     KAMQQRAQNDEVVKKQLINDKMSDAMQDFDEIVNSDDAWSETLEITSKGTFKASIPNI
                     EIILRNDPNLKGKIAFNEFTKQIECLGKVPWNTNFKTRQWQDGDDSSLRSYIEKIYDI
                     HHSGKTKDAIISVAMQNAYHPVRDYLNKISWDGHKRLEKLFIKYLGVEDTEVNRTTTK
                     KALTAGIARVMEPGCKFDYMLTLYGPQGVGKSALLKKLGGAWFSDSLVSVTGKEAYEA
                     LQGVWLMEMAELAATRKAEVEAIKHFISKQVDRFRVAYGHYIEDFPRQCIFIGTTNKV
                     DFLRDETGGRRFWPMTVNPERVEVNWSKLTKEEIDQIWAEAKYYYEQGEELFLNPELE
                     EEMRSIQSKHTEESPYTGIIDEYLNTPIPSNWDDLTIFERRRFYQGDVDMLPTGNVDY
                     VERNKVCALEVFVECFGKDKGDSRGSMEIRKISNILRQLDNWSVYDGNKSGKIRFGKD
                     YGVQIAYVRDESLEDLI"
     gene            complement(19929..20027)
                     /locus_tag="NBH32_000238"
     CDS             complement(19929..20027)
                     /locus_tag="NBH32_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513877.1"
                     /translation="MNLMQHWKQTGLNLTARRERGVVSFVASMLHH"
     gene            20161..20451
                     /locus_tag="NBH32_000239"
     CDS             20161..20451
                     /locus_tag="NBH32_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VRR-NUC domain-containing protein"
                     /protein_id="HCD1513878.1"
                     /translation="MKESTLEKYLVKEITKLNGLCLKWVAPGTRGVPDRIIIMPEGKT
                     YFVEMKQEKGKLHPLQKYVHRQFENRDHKVYVLWNKEQVNTFIRMVGGTFGD"
     gene            20432..21799
                     /locus_tag="NBH32_000240"
     CDS             20432..21799
                     /locus_tag="NBH32_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1513879.1"
                     /translation="MEHLAIDFKPHSYQKYAIDKVIDNEKYGLFLDMGLGKTVSTLTA
                     FSELQLLDTKKMLVIAPKQVAKDTWVDEVDKWNHLNHLKVSLVLGTPKERNDALNTEA
                     DIYVTNKENTKWLCDQYKKEWPFDMVVIDELSTFKSPKSQRFKSIKKKLPLINRFIGL
                     TGTPSPNSLQDLWAQVYLIDRGERLESSFSRYRERYFKPTHQVSEHIFKWELRDGSEE
                     KIYKQIEDICLSMKAKDYLDMPDRVDTKQTVVLSEKERKVYEELEKNYILESEEEGTV
                     VAQNGASLSQKLLQLSNGAVYTDEEDVRLIHDKKLDKLEEIIEESQGQSILLFYNFKH
                     DKERILQRFKEATTLEDSNYKERWNSGDIKLLIAHPASAGHGLNLQQGGHIIVWFGLT
                     WSLELYQQANARLYRQGQNHTTIIHHIMTDNTIDQRVYKALQNKELTQEELMKAIKAR
                     IAKHK"
     gene            21812..22249
                     /locus_tag="NBH32_000241"
     CDS             21812..22249
                     /locus_tag="NBH32_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1513880.1"
                     /translation="MGKASYDIKPGTFKYIESEIYNLNENKKEINRLRMEILNPTKEL
                     DTNIVYGPLQKGEPVRTTELMATRLLTNKMLRNLEEMVEAVESEYLKLPEDHKKVIRL
                     KYWNKDKKLKIEQIGDACHMHRNTVTTIRKNFVKAIAYHAGIK"
     gene            22406..22720
                     /locus_tag="NBH32_000242"
     CDS             22406..22720
                     /locus_tag="NBH32_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1513881.1"
                     /translation="MTKHNNIYKHGRKSYQYDWFYHSKAWKKLREIALDRDNYLCQMC
                     LREDIITDAKIVHHIIYVDEDFNKALDLDNLMSVCYSCHNKIHANDNDKSNLKKIRVL
                     KI"
     gene            22849..23154
                     /locus_tag="NBH32_000243"
     CDS             22849..23154
                     /locus_tag="NBH32_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P27 family phage terminase small subunit"
                     /protein_id="HCD1513882.1"
                     /translation="MKLTKKQLKEYIEDYKKSDDILINLYIETYEFYCRLRDELKNSD
                     LMIEHTNKAGASNIIKNPLSIELTKTVQTLNNLLKSMGLTAAQRKKIVQEEGGFGDY"
     gene            23144..24835
                     /locus_tag="NBH32_000244"
     CDS             23144..24835
                     /locus_tag="NBH32_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD1513883.1"
                     /translation="MTIKVLNEPSPKLLTTWYAEQVTQGKIKTSKYVRKECERHLRYL
                     ENGGKWVFDEELAHRPIRFIEKFCKPSKGSKRQLVLQPWQHFIIGSLFGWVHKETKLR
                     RFKEALIFMGRKNGKTTTISGVANYAVSQDGENGAEIHLLANVMKQARILFDESKAMI
                     KASPKLDKNFRTLRDEIHYDATISKIMPQASDSDKLDGLNTHMGIFDEIHEFKDYKLI
                     SVIKNSRAARLQPLLIYITTAGYQLDGPLVDMVEAGRDTLDQIIEDERTFYYLASLDD
                     DDDINDSSNWIKANPNLGVSINLDEMKEEWEKAKRTPAERGDFITKRFNIFANNDEMS
                     FIDYPTLQKNNEIVSLEELEGRPCTIGYDLSETEDFTAACATFALDNGKVAVLSHSWI
                     PKHKVEYSNEKIPYREWEEDGLLTVQDKPYIDYQDVLNWIIKMNEHYVVEKITYDRAN
                     AFKLNQELKNYGFETEETRQGALTLSPALKDLKEMFLDGKIIFNNNPLMKWYINNVQL
                     KLDRNGNWLPSKQSRYRKIDGFAAFLNTYTDIMNKVVSDKGEGNIEFISIKDIMR"
     gene            24840..26078
                     /locus_tag="NBH32_000245"
     CDS             24840..26078
                     /locus_tag="NBH32_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1513884.1"
                     /translation="MNVIAKENIVTRIKKKLIDNWIDQSASKLYDFSPWKNKSFWGVI
                     NNTLETNETIFSAITKLSNSMASLPLKMYEDYKVVNTEVSDLLTVSPNNSLSSFDFIN
                     QIETIRNEKGNAYVLIERDIYHQPSKLFLLNPDVVEMLIENQSRELYYSIHAATGNKL
                     IVHNMDMLHFKHIVASNMVQGISPIDVLKNTTDFDNAVRTFNLTEMQKPDSFMLKYGS
                     NVGKEKRQQVLEDFKQYYEENGGILFQEPGVEIEPLPKKYVSEDIVASENLTRERVAN
                     VFQLPSVFLNARSNTNFAKNEELNRFYLQHTLLPIVKQYEEEFNRKLLTKTDREKNRY
                     FKFNVKSYLRADSATQAEVYFKAVRSGYYTINDIREWEDLPPVEGGDKPLISGDLYPI
                     DTPLELRKSLKGGDKNVNES"
     gene            26062..26835
                     /locus_tag="NBH32_000246"
     CDS             26062..26835
                     /locus_tag="NBH32_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1513885.1"
                     /translation="MSMKAKYFQMKRKSKSKGEIFIYGDIVSDKWFESDVTATDFKNK
                     LDELGDISEIDVHINSSGGSVFEGHAIYNMLKMHPAKINIYVDALAASIASVIAMSGD
                     TIFMHKNSFLMIHNSWVMTVGNAEELRKTADLLEKTDAVSNSAYLDKAKDLDQEHLKQ
                     MLDAETWLTAEEALSFGLIDEILGANEIAASISKEQYKRFENVPEDLKKDVDKITKID
                     DVDTSELVETPKESMSLEEKEKREKIKRECEILKMTMNY"
     gene            26847..28010
                     /locus_tag="NBH32_000247"
     CDS             26847..28010
                     /locus_tag="NBH32_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD1513886.1"
                     /translation="MPTLYELKQSLGMIGQQLKNKNDELSQKATDPNIDMEDIKQLET
                     EKAGLQQRFNIVERQVQDIEEKEKAKVKDKGEAYQSLSDNEKMVKAKAEFYRHAILPN
                     EFEKPSMEAQRLLHALPTGNDSGGDKLLPKTLSKEIVSEPFAKNQLREKARLTNIKGL
                     EIPRVSYTLDDDDFITDVETAKELKAKGDTVKFTTNKFKVFAAISDTVIHGSDVDLVN
                     WVENALQSGLAAKERKDALAVSPKSGLEHMSFYNGSVKEVEGADMYDAIINALADLHE
                     DYRDNATIYMRYADYVKIISVLSNGTTNFFDTPAEKVFGKPVVFTDAAVKPIVGDFNY
                     FGINYDGTTYDTDKDVRKGEYLFVLTAWYDQQRTLDSAFRIAKAKENTGPLPS"
     gene            28079..28357
                     /locus_tag="NBH32_000248"
     CDS             28079..28357
                     /locus_tag="NBH32_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HCD1513887.1"
                     /translation="MSLEEIKLWLRIDYNFENDLIEGLIQSAKSELLLSGVPDYDKDD
                     LEYPLFCTAIKYIIARDYESRGYSNDQSRSKVFNEKGLQKMILKLKKW"
     gene            28369..28701
                     /locus_tag="NBH32_000249"
     CDS             28369..28701
                     /locus_tag="NBH32_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513888.1"
                     /translation="MEFNEFKDRAYFFQYINKGPYPDEEEKMKLYSCFCKIYNPSMKD
                     REILKATESKSGLTIIVRSSKTEYLPQTNHLVKIDSALYSDKLFNIVEIRIDTPDIGY
                     NTVVLSEK"
     gene            28698..29099
                     /locus_tag="NBH32_000250"
     CDS             28698..29099
                     /locus_tag="NBH32_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513889.1"
                     /translation="MSVEIKGIPEVLNKLESVYGKQAMQAKSDKALNEASEFFIKALK
                     KEFESFKDTGASIEEMTKSKPYTKVGSQERAVLIEWVGPMNRKNIIHLNEHGYTRDGK
                     KYTPRGFGVIAKTLAASERKYREIIKKELAR"
     gene            29100..29495
                     /locus_tag="NBH32_000251"
     CDS             29100..29495
                     /locus_tag="NBH32_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3168 domain-containing protein"
                     /protein_id="HCD1513890.1"
                     /translation="MNILNTIKGILLSDAELKTHINSRIYYYKVTENAETSKPFVVIT
                     PVYDLPSDFMSDKYLSEEYLIQIDVESSNNQKTIDITKRIRYLLYQQNLIQASSQLDA
                     YFEETKRYVMSRRYQGIPKNIYYKNQRIE"
     gene            29530..30171
                     /locus_tag="NBH32_000252"
     CDS             29530..30171
                     /locus_tag="NBH32_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1513891.1"
                     /translation="MAEGQGSYKVGFKRLYVGVFNPEATKVVKRMTWEDEKGGTVDLN
                     ITGLAPDLVDMFASNKRVWMKKQGTNEVKSDMSIFNIPSDDLNTVIGRTKDKNGTSWV
                     GENTRAPYVTVIGESEDGLTGQPVYVALLKGTFSLDSIEFKTRGEKAEAPEPTKLTGD
                     WMNRKVDVDGTSQGIVYGYHEGKEGEAEFFKKVFVGYTDSEDHSEDSAGSLPS"
     gene            30263..30718
                     /locus_tag="NBH32_000253"
     CDS             30263..30718
                     /locus_tag="NBH32_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HCD1513892.1"
                     /translation="MTKTLKVYKGDDVVASEQGEGKVSVTLSNLEADTTYPKGTYQVA
                     WEENGKESSKVDVPQFKTNPILVSGVSFTPETKSIMVNTDDNVEPNIAPSTATNKILK
                     YTSEHPEFVTVDENTGAIHGVAEGTSVITAMSTDGSDKSGQISVTVTNG"
     gene            30776..31126
                     /locus_tag="NBH32_000254"
     CDS             30776..31126
                     /locus_tag="NBH32_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513893.1"
                     /translation="MIKFEIKDRKTGKTESYTKEDVTMGEAEKCYEYLELVNQENKKE
                     APNATKMRQKERQLLVDLFKDEGLTEEDVLNKMSTKTYTKALQDIFREINGEDEEDSE
                     TEPEEMGKTEEQSQ"
     gene            31168..31326
                     /locus_tag="NBH32_000255"
     CDS             31168..31326
                     /locus_tag="NBH32_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513894.1"
                     /translation="MEQYGWTLTEVRKQPYVKLLEILNEENKEETEEKQSEQKVITGT
                     DLRKLFGS"
     gene            31340..37516
                     /locus_tag="NBH32_000256"
     CDS             31340..37516
                     /locus_tag="NBH32_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1513895.1"
                     /translation="MNEKVEGMTLELKLDHLGVQEGMKGLKRQLGVVNSEMKANLSAF
                     DKSEKSMEKYQARIKGLNDRLKVQKKMYSQVEDELKQVNANYQKAKSSVKDVEKAYLK
                     LVEANKKEKLALDKSKEALKSSNTELKKAENQYKRTNQRKQDAYQKLKQLRDAEQKLK
                     NSNQATTAQLKRASDAVQKQSAKHKALVEQYKQEGNQVQKLKVQNDNLSKSNDKIESS
                     YAKTNTKLKQTEKEFNDLNNTIKNHSANVAKAETAVNKEKAALNNLERSIDKASSEMK
                     TFNKEQMIAQSHFGKLASQADVMSKKFSSIGDKMTSLGRTMTMGVSTPITLGLGAALK
                     TSADFEGQMSRVGAIAQASSKDLKSMSNQAVDLGAKTSKSANEVAKGMEELAALGFNA
                     KQTMEAMPGVISAAEASGAEMATTATVMASAINSFGLKASDANHVADLLARSANDSAA
                     DIQYMGDALKYAGTPAKALGVSIEDTSAAIEVLSNSGLEGSQAGTALRASFIRLANPS
                     KNTAKEMKKLGIHLSDAKGQFVGMGELIRQFQDNMKGMTREQKLATVATIVGTEAASG
                     FLALIEAGPDKINSYSKSLKNSNGESKKAADLMKDNLKGALEQLGGAFESLAIEVGKD
                     LTPMIRAGAEGLTKLVDGFTHLPGWVRKASVGLALFGAAIGPAVLAGGLLIRTVGSAA
                     KGYASLNRRIAENTILSNTNSKAMKSLGLQTLFLGSTTGKTSKGFKGLAGAMMFNLKP
                     INVLKNSAKLAILPFKLLKNGLGLAAKSLFAVSGGARFAGVALRFLTGPIGATITAIT
                     IAYKVFKTAYDRVEWFRNGINGLGETIKFFGGKIIGGAVRKLGEFKNYLGSIGKSFKE
                     KFSKDMKDGYKSLSDDDLLKVGVNKFKGFMQTMGTASKKASDTVKVLGKGVSKETEKA
                     LEKYVHYSEENNRIMEKVRLNSGQITEDKAKKLLKIEADLSNNLIAEIEKRNKKELEK
                     TQELIDKYSAFDEQEKQNILTRTKEKNDLRIKKEQELNQKIKELKEKALSDGQISENE
                     RKEIEKLENQRRDITVKELSKTEKEQERILVRMQRNRNAYSIDEASKAIKEAEKARKA
                     RKKEVDKQYEDDVIAIKNNVNLSKSEKDKLLAIADQRHKDEVRKAKSKKDAVVDVVKK
                     QNKDIDKEMDLSSGRVYKNTEKWWNGLKSWWSNFREDQKKKSDKYAKEQEETARRNRE
                     NIKKWFGNAWDGVKTKTGEAFSKMGRNANHFGGEMKKMWSGIKGIPSKLSSSWSSAKS
                     SVGYHTKAIANSTGKWFGKAWQSVKSTTGSIYNQTKQKYSDASDKAWAHSKSIWRGTS
                     KWFSNAYKSAKGWLTDMANKSRSKWDNISSTAWSNAKSVWKGTSKWFGNSYKSLKGWT
                     GDMYSRAHDRFDAISSSAWSNAKSVFNGFRKWLSKTYDWIRDIGKDMGRAAADLGKNV
                     ANKAIGGLNSMIGGINKISKAITDKNLIKPIPTLSTGTLAGKGVATDNSGALTQPTFA
                     VLNDRGSGNAPGGGVQEVIHRADGTFHAPQGRDVVVPLGVGDSVINANDTLKLQRMGV
                     LPKFHGGTKKKKWMEQVTENLGKKAGGFGSKAKNTAHNIKKGAEEMVEAAGDKIKDGA
                     SWLGDKIGDVWDYVQHPGKLVNKVMSGLNINFGGGANATVKIAKGAYSLLKKKLVDKV
                     KSWFEDFGGGGDGSYLFDHPIWQRFGRYTGGLNFNGGRHYGIDFQMPTGTNIYAVKGG
                     IADKVWTDYGGGNSIQIKTGANEWNWYMHLSKQLARQGQRIKAGQLIGKSGATGNFVR
                     GAHLHLQLMRGSHPGNDTAVDPMKWLKSLKGGGGKVGGSGYENAKRAILRAQSILGGR
                     YRSDYITTQMLRVAKRESNYQADAINNWDSNARAGTPSKGMFQMIEPSFRAFAKPGHG
                     NIYNPTDEAISAMKYIVAKYGWGGFKRAGDYAYANGGLITKHQIAEVGEGDKPEMVIP
                     LTRRKRAMQLTEQVMRIIGMDGKPNNITVNNDTSTVEKLLKQIVMLSDKGNKLTDALI
                     QTVSSQDNNLGSNDAIRGLEKILSKQSGHRANANNYMGGLTN"
     gene            37516..38340
                     /locus_tag="NBH32_000257"
     CDS             37516..38340
                     /locus_tag="NBH32_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD1513896.1"
                     /translation="MQSFVKIIDGYKEEVITDFNQLIFLDARAESPNTNDNSVTINGV
                     DGILPGAISFAPFSLVLRFGYDGIDVIDLNLFEHWFRSVFNRRHPYYVITSQMPGVKY
                     AVNTANVTSNLKDGSSTEIEVSLNVYKGYSESVNWTDSEFLFDSNWMFENGIPLDFTP
                     KYTHTSNQFTIWNGSTDTINPRFKHDLKILINLNASGGFELVNYTTGDIFKYNKSIDK
                     NTDFVLDGVYAYRDINRVGIDTNRGIITLAPGKNEFKIKGDVSDIKTTFKFPFIYR"
     gene            38349..39932
                     /locus_tag="NBH32_000258"
     CDS             38349..39932
                     /locus_tag="NBH32_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1513897.1"
                     /translation="MDYHDHLSVMDFNELICENLLDVDYGSFKEYYELNEARYITFTV
                     YRTTHNSFVFDLLICENFIIYHGEKYTIKQTAPKVEGDKVFIEVTAYHIMYEFQNHSV
                     ESNKLDDDSSETGKTPEYSLDEYLRYGFANQKTSVKMTYKIIGDFKRKVPIDELGNKN
                     GLEYCKEAVDLFGCIIYPNDTEIGFYSPETFYQRSEKVIRYQYNTDTVSATVSTLELR
                     TAIKVFGKKYTAEEKKNYNPIRTTDIKYSNGFIKEGTYRTETIGSKATINFDCKYGNE
                     TVRFTIKKGSQGGIYKLILDGKQIKQISCFAKSVQSETIDLIKNIDKGKHVLEMIFLG
                     EDPKNRIDISSNKKAKPCMYVGTEKSTVLNLIADNSGRNQYKAIVDYVADSAKQFGIR
                     YANTQTNEDIETQDKLLEFAKKQINDTPKTELDVNYIGYEKIEPRDSVFFVHELMGYN
                     TELKVVKLDRSHPFVNAIDEVSFSNEIKDMVQIQQALNRRVIAQDNRYNYQANRINHL
                     YTSTLNSPFETMDIGSVLI"
     gene            39932..40222
                     /locus_tag="NBH32_000259"
     CDS             39932..40222
                     /locus_tag="NBH32_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513898.1"
                     /translation="MATEEVKIKALLENDKQYFPATHWKAINGIPYAGSSDIDGLPQD
                     GIISVDDKNKLDKLKIGEAGIIQNSIVQKSPNGKLWKITVDDSGKLGTVLFY"
     gene            40238..42148
                     /locus_tag="NBH32_000260"
     CDS             40238..42148
                     /locus_tag="NBH32_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513899.1"
                     /translation="MENLYLIKDLGALAGRDYRAKEIQNLQRIEQFALGLTTEFKLHQ
                     KAKTMQHFAEQIYYNGRSQAAVNKSLQSQINALVVAPRNNSANEIVQARVNVNGETFD
                     TLKEHLDDWETKTQINKEETIRELNKTKQEILDIEYRFEPDKQEFLFVTELAPLTNAV
                     MQSFWFDNRTGIVYMTQARNNGYMLSRLRPNGQFIDSSLIVGGGHGTHNGYRYIDDEL
                     WIYSFILNGNNENTLVRFKYTPNVEISYGKYGMQDVFTGHPEKPYITPVINEKENKIL
                     YRIERPRSQWELENSMNYIEIRSLDDVDKNIDKVLHKISIPMRLTNETQPMQGVTFDE
                     KYLYWYTGDSNPNNRNYLTAFDLETGEEAYQVNADYGGTLDSFPGEFAEAEGLQIYYD
                     KDSGKKALMLGVTVGGDGNRTHRIFMIGQRGILEILHSRGVPFIMSDTGGRVKPLPMR
                     PDKLKNLGMLTEPGLYYLYTDHTVQIDDFPLPREWRDAGWFLEVKPPQTGGDVIQILT
                     RNSYARNMMTFERVLSGRTGDISDWNYVPKNSGKWERVPSFITKMSDINIVGMSFYLT
                     TDDTKRFTDFPTERKGVAGWNLYVEASNTGGFVHRLVRNSVTASAEILLKNYDSKTSS
                     GPWTLHEGRIIS"
     gene            42148..43614
                     /locus_tag="NBH32_000261"
     CDS             42148..43614
                     /locus_tag="NBH32_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HCD1513900.1"
                     /translation="MSNLEKSVAINLENTAHYENISNLDITFRTGESDSSVLLFNIIK
                     NNQPLLLSEENIKARIAIRGKGVMVVAPLEILDPFKGILKFQLPNDVIKRDGSYQAQV
                     SVAELGNSDVVVVERTITFNVEKSLFSMIPSETKLHYIVEFQELEKTIMDRAKAMDEA
                     IKNGEDYASLIEKAKEKGLSDIQIAKSSSIDELKQLANSHISDLENKAQAYSRTFDEQ
                     KRYMDEKHEAFKQSVNSGGLVTSGSTSNWQKAKITKDDGKIMQITGFDFNNPEQRIGD
                     STQFIYVSQAINYPRGVSTNGTVEYLVVTSDYKRMTYRPNGTNKVFVKRKEAGSWSEW
                     SELAINDYNTPFETVQSAQSKANMAESNAKLYADDKFNKRYSVIFDGTANGVGSTLYL
                     NESLDQFILLIFYGTFPGGDFTEFGSPFGGGKISLNPSNLPDGDGNGGGVYEFGLTKS
                     SRTSLTISNDVYFDLGSQRGSGANANRGTINKIIGVRK"
     gene            43614..44003
                     /locus_tag="NBH32_000262"
     CDS             43614..44003
                     /locus_tag="NBH32_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2977 domain-containing protein"
                     /protein_id="HCD1513901.1"
                     /translation="MQILVNKRNEIISYAIIGGFEEGIDIENLPENFSQVFRPKAFKY
                     SNGEIVFNEDYSEEKDDLHQQIDSEEQNTVASDDILRKMVASMQKQVVQSTKLSMQVN
                     KQNALMAKQLVTLNKKLEEVKGETENA"
     gene            43996..44160
                     /locus_tag="NBH32_000263"
     CDS             43996..44160
                     /locus_tag="NBH32_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HCD1513902.1"
                     /translation="MLKLISPTFEDIKTWYQLKEYSKEDIAWYVDMEVIDKEEYAIIT
                     GEKYPENLES"
     gene            44206..>44344
                     /locus_tag="NBH32_000264"
     CDS             44206..>44344
                     /locus_tag="NBH32_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 family protein"
                     /protein_id="HCD1513903.1"
                     /translation="MFGFTKRHEQDWRLTRLEENDKTMFEKFDRIEDSLRTQEKIYDK
                     LD"
BASE COUNT        16466 a         6255 c         8605 g        13018 t
ORIGIN      
        1 ttgccatttg tatgatgatt aaatgtatag tcaagtgctt tgtcaatttc gtctattgtg
       61 aaaatatggt cgtcgatgtc tggtatgata ttgttttgtt cgatgatttt atttaaaatt
      121 tttaattgct caccatccga acgtacaaat ataaaacgat aatctatcga ctttttttga
      181 gcttttttat cgaatttttt acctgctaaa ccaaatagca attttttaaa tggtgaaaaa
      241 ttatttttca cagcaaatgc tttgttaggc gcattaataa gactgacaat agtaccacca
      301 ggtttcataa ctgataattc tttatcgaat tcatttggtc caagtgtgtc tataacataa
      361 tcaatgtcag ataaaacttc acagtaattc tcagttttgt agtctatata ttgatcgaca
      421 cccttagcta aaaattgatc tttaagtctc ggacttccgg atacaataac tttaagccct
      481 aatgattttg ctataggtac tgccatttgt ccaaagctgc ctgaaccacc aggtataaac
      541 aatgttttac caggtatagc ttttaattct tcagtaattg cttgataagc agttaaacct
      601 gttaacggca ctgctgcacc tgttttaagg tttaaatttt taggtaattt tgcaatagca
      661 ttagattcaa tagaaacata ctctgcaaaa gcacctatac gatcgattgg taatcgtgaa
      721 tatattttat cgcctaattt ataatcttcg actttacttc ctactttaac aattgttcct
      781 gtgagttcat taccaatagt ttgaggcatt ttatagtttt gaattaattt gacacttcca
      841 ttaatagtta aattttctaa tggattaatt gctgcataag ctacttttac aagtacctca
      901 gtagatttga tctctggaat agtaatatct ctaatttgta catctctatt tttattgtat
      961 ttctttattt gaatggcttt catagtatat atggctcctc agtgattaaa atgcattaca
     1021 atattttcat tagaccccac aaatatttca ctctataagt atattatcaa tttataaatg
     1081 taacttttca gcatggtgca gttgtataaa gctataaaat caattaactc cacatattta
     1141 ataatacatc agcaaagcct tcaggttttt gaatataacc taagtgacca cctggaatat
     1201 ctacaatagg tatgccagtt tcgttattta tataaaagtt aacatcttgt gggaaggagc
     1261 ctctagaatc tgtcccattt agtagggtga ttttatcgtt gtattttgtg aaatcatcca
     1321 aagtaatatc tgaatgcgta tattgtctaa tttcaaattc tgaccagaac atcgtacgtt
     1381 tgtactgttc tatacgtcct tcttcagtat cagcaggttg agacatcatt tttgcatcaa
     1441 ttggtgcgat atttaatgtt tcaccaaatg ttttcatgcc tttttctaag ccttctgtta
     1501 aaatttgatg cacaatgtca tcatttttat ctttccaata agtactgtct ggtaaaaatg
     1561 tattaattgg tggttcgtga aatgcaatct ttttaacgac ttcagggtaa tcttttaaca
     1621 catgcatcgc aacgattgaa cctgaacttg aacctaatat atagacaggt tcatcactta
     1681 atgactttgc aagttcggca atgtcctgtg cgtcgcgttt gacacgataa tcactgtcag
     1741 ggttagaagc ggaatcagga agtggttcag ttaactcgct ttctccataa tcacgacgat
     1801 caacggctac aacagtaaaa tggtctttta actgttctgc aagaggcaga aaaatgtctc
     1861 cggtaccgtt tgcaccagga ataaagatga gcacgggtcc ttgtccgact tggtggtatc
     1921 gtaatttagc gccttgtaat tctaaagttt ccatattcaa tgacctccat ttgttaattg
     1981 ttagatgata aacctaataa tttagcacca tttgtataac ttattttctc tttttcttca
     2041 tctgttaaac ccagttcatc taaaaataca cctaattttt caggctcaat atatggataa
     2101 tcagcagcat aaagaattct atcaatacct acttctttct tgactaaatc aaactgtggc
     2161 ttcgttaaca tgccactcgg tgtgatataa aaattatttt taaagtaata gcttacaggg
     2221 tggttcaaat gttcagcgaa taaagcttca tccatacgtt ctaagaagaa ggggataaac
     2281 tcaccccaat gtccaataat catatttaac tttggataac gatcaaaaat gccagataat
     2341 acaagatgta ttgcatgaat accgacatca atgtgccaac cataaccaaa acaagcaaat
     2401 gttgccgcag ttacttcagg ataatttcct ttatagtatg attgataaat gtcactgtta
     2461 actggcgcgg gatgtagata aatcggtacg tctaaatttt cagctgtttt gaaaataatg
     2521 tcatatttgt cttgatcaag aaaaccatct tgtgcacgtc ccataatgag cgcacctttg
     2581 aatcctaaat cattgatgca acgttcgaat tctcgcgctg cggcttcagg ctcattgata
     2641 ggtaaagttg caaagcctac aaagcgattg ggatattgtg caatataatt tgccaattga
     2701 tcatttgctt tttgacataa ttcaatggct ttctgaccaa ctaaatttga aggagaccca
     2761 tttccataag ataagacttg aatttgaacg tcttgattat tcataaattg gatacgttca
     2821 ttatgatgat atgtttattt caagaaaagc tttaacgcca gtgttctcaa gcgttttata
     2881 aagcttgtaa aaaatataag ggcaaaaaaa gggcggattt aagctaactt ggaatgtttt
     2941 cgagtttttg agttagttct ctatccattt tttcagttac atgagtatat atgcgaatgg
     3001 ttgttttttc atctacatgt cctacccttt tcataattgc ttttaaagaa acattcattt
     3061 ctactaataa agttatgtgt gtatgtctaa atgtgtgcgt ggtaactttc ttattcatat
     3121 ttaaagcttt tgtagttttc ttaagcacac cggcgatttg attattacat aaaggattcc
     3181 ctttttttgt tgtgaatatg aaccctctgt caacatagct cgaattccat cttttcaaca
     3241 ttttgttttc cagtattatc tttttaaaaa tttctacggt tctagaattg atgctgatac
     3301 ttctttttga acttatagtc tttgtagtgt ctttgtatcc gaatccttcc tcgtatttaa
     3361 tgcggtgaat tgtacctgtt atattgatag ttttgtttaa taaatctata tctttttcct
     3421 gcagtgcttg tagttctcct atgcgcatac cagttaaagc ctgtacttct aagatgctgg
     3481 caattaaaat gcgatttcgc ttgtgtaact tattatcatt tagtatatga tcacgtatct
     3541 gtaggacttg gttcatttct aaatagttgt acattttaga ttcatctttt tcgatatcct
     3601 ctattgtttt tcttctttta ggaattttga cattagttaa caaatattca tttggataat
     3661 tgtaaaattt aactgcatat ttaatagctc ctttcatatc tccgagttga cgggttactt
     3721 gattttgaga atagatatct gataatttat taataaatat ctgcatatat tttgtatcta
     3781 gtttgtttaa aagcaagttc tcagagctgt atcgtttaat gtttctaatt cttattttta
     3841 tattattaag agtagtcaac tttgaacctg atgtttttat atgatattca agccattcat
     3901 ctaatagcgc gtgaaaagtc aaagttttta attcgcttga cgacttgttg ttcagttttt
     3961 cttttatttt ttcttctaaa cgaaacattg cttctttttg agattgtttt gtattcttgt
     4021 tcaacacaac acttacgcgc ttccatttat ctgtgtatgg atctttgtac ttctcgtaat
     4081 atctgtattt agtttcgtta tttttgtttt taaatttttc aatccacatg tttatacctc
     4141 ctgtaggaac gtacgttctg taaatttgta aaaaataata agggtaggtg ggctacccaa
     4201 aatttagtac taggtactaa atatgttata ataaaataaa aagtaggtga taagatgact
     4261 caatttctag gggcgcttct tcttacagga gttttaggtt acataccata taaatatcta
     4321 acaatgatag gtttagttag tgaaaaaaac aaggttatca atactcctgt attattgatt
     4381 ttttctattg aaacatgttt gatatggttt tatagtttta taatttttaa taatgttgat
     4441 ttaaaaaatt tgaatttaat tcagttgctt acaggtctaa aagcaaatat tttgtttcta
     4501 tttatttttg ttttaacagt gtttgtattt aatcctttaa ttgttaaatt tattatctgg
     4561 ttaattaata taaccagaaa gtttatgaaa ttggattgta taagcttatt agacaaaaga
     4621 gacaagttgt ttaataacaa cggtaaacca gtatttatag ttataaaaga ctttgaaaac
     4681 agaatcattg aagagggtga acttaaaacc tataattcag ctggtagcga tttcgattta
     4741 ctagaagttg agcgacaaga tttcaaagta tctgatttac cgtcaaacga tgaattgtat
     4801 attaaacata cgcttgtaga ccttaaacaa caaattaaat tggatttata tttaatgaat
     4861 gaatactaat cttttttctt agctttttct gataaagtgc tttttaattt ttcgctggcg
     4921 cctgactttt caaaactttt gtttaatggg ttactacgag tagtttcttg ttttttgttt
     4981 ttatctacca taaaattctc accaccattc aacgtctaca ctagtaggcg tttttttatt
     5041 tagtaaaatc ataatgaatc ttctttggtt aacttatctc catctatttt ttgtgaaata
     5101 aattccaagt atttacgcgc attatgtgac gataaatctt taggtaactc ataagtgaat
     5161 ggttgattac cactagttaa aacttcatat actatagttt ctttttttat tttgcaatta
     5221 gttattttca ttataaactt cctttcaaac actgctgaaa tagacgtctt ttatattaaa
     5281 gcgccacaca ggcgctgtta atcacaatac aactttgccc attactttaa tattactaaa
     5341 cgaagcgact ttgatatcat catacttcgg atttagagat accaaattaa tatagtcttc
     5401 gcatatatct acacgcttga taagacttac tccatctaat acaacgagtg caattgtacc
     5461 atctttaata gaatcttctt tcttaataaa agcgtatgtt ccttgtttta acataggttc
     5521 cattgaatca ccattaacta aaatacaaaa atcagcattt gatggcgttt cgtcttcttt
     5581 aaaaaatact tcttcatgca atatgtcatc atataattct tctcctatgc cagcaccagt
     5641 tgcaccacat gcaatatacg atactagttt agactcttta tatccatcta tagaagtgac
     5701 tttattttgt tcatctaact gactattcgc gtagttgagt acattgcttt gtcttggagg
     5761 cgtgagctga gatgatatgt tattaatttt tgacataaca gtttcctctt ggcgttcttc
     5821 atcgggtacg cgataagaat ttacatcata tcccataagc cacgcttcac cgacatttaa
     5881 agttttagaa agtaggtaaa ttctatcttg gtcaggagat tgtacatcgt taatatattg
     5941 agacaaagta cttttactta aagatatacc tagttgcttt tgataaggtt tcgatttatt
     6001 aatgatatct acttgtttta aatttcttat tttcatgatg tgttttagtc tgtttgaaac
     6061 tttttctctc atttagtgca cctccgtttg ataactttat aataaacctt gttgaacaaa
     6121 aattcaataa aaaagttcat aaaacatgaa tttttgtgtt gacttaattc aaaacaaggt
     6181 gtaaagtata gttaagttca cgatacatga acttcaaagg aggtgttttt tatgtgttac
     6241 gactactcgc gtttgagtgg taagatagtt gaaaagtatg gcactcagta caatttcgct
     6301 attgctatga agttgtccga gagaagctta tccttaaaac tcaacggaaa agttgggtgg
     6361 aaagatagcg aaatatggaa agctatacaa ttgctaggta taccggtaga aaaaatacat
     6421 ttatattttt ttaaagaaaa agttcacgtt tgatgaacta gaaagtggag gacatcatgg
     6481 aacaaatcac attaaccaaa gaagagttga aggaaattat agcgaaagaa gttagagaag
     6541 ctataaatgg caagaaacca atcagttcag gtgcaatttt cagtaaagta agaatcaata
     6601 atgacgattt agaagaaatc aataaaaaac tcaatttcgc aaaagatttg tcgctaggaa
     6661 gattgaggaa gctcaatcat ccgattccgc taaaaaagta tcagcatggc ttcgaatcaa
     6721 ttcatcaaaa agcttatgta caagatgttc atgaccatat tagaaaatta acattatcaa
     6781 tttttggagt gacacttaat tcagacttga gtgaaagtga atacaaccta gcagcaaaaa
     6841 tttatagaga tatcaaaaac tattatttat atatctatga aaagagagtt tcagaattaa
     6901 ctatcgatga tttcgaatga aggaggaact acaaatgaaa ctactaagaa ggctattcaa
     6961 taaaaaacac gaaaacttaa ttgacgtgtg gcatggaaat caatggttaa aagtgaaaga
     7021 aagcaaatta aaaaaatata aagtggtctc ggatagagaa ggtaagaaat atctaattaa
     7081 ataagcgcac ttaattagtg caagtaatca agtgcgctat tgccttacaa tcctaaatct
     7141 tttctgcttt tttcttcttc ttgtaatccc aataacacag aagagtaaat gctgaaatag
     7201 tcacgagcaa cgctatcttt agcgaatgca attacgtcat caccgacttc ttgccattcg
     7261 ttatgaatct tatgtctatc tagagctcta ggtaatagcg agattgtaat atcgtgagca
     7321 attttctcta aatccataaa tttcacctcc ttccactggg agataactaa attatataac
     7381 aaaacaactt aaaggaggaa cgacaaatgc aagctcaaaa caaaaaagtc atctattact
     7441 actatgacga agaaggtaat aggcgaccat tagatattca aattaatgac ggatatgaac
     7501 tgatggtccg atctcatttc atcaacaaca ccattgaaga aataccatac gtaaataata
     7561 acttatatgc cttggttgat ggttatgaat ttaagttaga ttgaattttt gagaaagata
     7621 ttgaaaagct aatttcccca taagattaag agacatactg gatgttttgt taacgactct
     7681 tttaacttcg ttccaagttt tattgtctct aatattatcg agaaattcat ggccagacca
     7741 agtgatgtca tcaataatcc aagaaacgac cctgccttcg atgaatttca gatcgcaaca
     7801 aataaattta gcttcttcta attttaaaag tgagtacatt actgtttcaa aatcatattt
     7861 atcaaaaata atattatcgt tgaaattatg tcgagtaagt ggttcaccta ttttcttatt
     7921 agattctatt tctaagagca agagtctaac gcaatcgtga ttaagtttca tcctatcacc
     7981 tccataacag gagtatagca gaaaggatca taaacatctt aaaaggagga ataacaaatg
     8041 aacattcaag aagcaactaa gatagctaca aaaaatcttg tctctatgac acggaaagat
     8101 tggaaagaaa gtcatcgaac taagatatta ccaacaaatg atagtttttt acaatgcatc
     8161 atttcaaata gcgatgggac aaaccttatc agatattggc aaccttcagc cgatgacctc
     8221 atggcaaatg attgggaagt tataaaccca actagagacc aggaattatt gaagcaattt
     8281 tagaaatgct atcaatgata ctttttaaat tgtttttaaa ctcattttca aagtaaacaa
     8341 cagtcttgtc tgaaattgtt acatgataaa tagtgttact agcatacacg ccgtttagga
     8401 acccagagtt tttaagttta tttaaatcgt attttacatc ttcgaaatgt agtttttgaa
     8461 aatactttgt atgtatatct ttagcacttc caaaattatt gcaggttaat ttaaccgaac
     8521 ctaactttac acattctaaa taatctttgt agagtacgga caagatatat tgttggtctt
     8581 tagtaagtgt atcaaattca tcagatatca agggcatgtt atcacctcct taggttgata
     8641 acaacattat acacgaaagg agcataaaca aatgaacaca agatcagaag gattgcgtat
     8701 aggcgtccca caagtttcta gcaaagctga tgcttcttca tcctatttaa cggaaaagga
     8761 acgtaactta ggagcggaaa tattagagct tattaaaaaa agtgattaca gctacttaga
     8821 aataaacaaa gttctctatg cattagatag agaacttcaa tacagggcga ataataacaa
     8881 actttaacat ttatctaaag gagtgataga gatgccaaaa atcataatac caccaacacc
     8941 agaaaacaca tatcgaggcg aagaaaaatt tgtgaaaaag ttatacgcaa cacctacaca
     9001 aatccatcaa ttgtttggag tatgtagaag tacagtatac aactggttga aatattaccg
     9061 tgaagataat ttaggtgtag aaaatttata cattgattat tcagcaacgg gaacattgat
     9121 taatatttct aaattagaag agtatttgat cagaaagcat aaaaaatggt attaggagga
     9181 ttatcaaatg agcgacacat ataaaagcta cctattagca gtgttgtgct tcacggtctt
     9241 agcgattgta ctcatgccgt ttctatactt cactacagca tggtcaattg cgggattcgc
     9301 aagtatcgca acattcatat tttataagga atacttttat gaagaataaa gaaactgcta
     9361 cttgttggag caagtaacag tgcaagatga gcaattgtct taaataatta tataaggagt
     9421 tattaatatg accttacaac aaaaaatact atcacatttt gcaacatatg acaatttcaa
     9481 ttctgatgat gttgttgaag tttttgggat atctaaaaca catgcaaaat ccacactttc
     9541 aagacttaag aaaaaaggaa agattgaatt ggaaagttgg ggtatctggc gtgttgttga
     9601 accgcagtta catttaactg ttgtagaacg taagaaagag atattagaag aacaattcga
     9661 gttattggca agattaaacg aacaaagtga tgaccctaga gaaatagaag aacgcatcaa
     9721 gttaatgatt cgtttagcca accaatttta aggaggagtt aatcaatggc aatattagaa
     9781 ggtatttttg aagaattaaa actattaaat aagaatttac gtgtgctaaa tactgaacta
     9841 tcaactgtag attcatcaat tgtacaagag aaagttaaag aagcaccaat gccaaaagat
     9901 gaaacagctc aactggaatc agttgaagaa gttaaggaaa cttctgctga tttaactaaa
     9961 gattatgttt tatcagtagg aaaagagttc cttaaaaaag cagatacttc tgataagaaa
    10021 gaatttagaa ataaacttaa cgaacttggt gcggataagc tatctactat caaagaagag
    10081 cattatgaaa aaattgttga ttttatgaat gcgagaataa atgcatgaag ctagatcact
    10141 caaatagagc tcatgcaaag cttagtgcaa gtggagcaaa acaatggcta aactgtccac
    10201 cgagtattaa ggcaagtgaa ggtattgcag ataaaagttc agtttttgct gaagaaggta
    10261 cattcgctca tgagttaagt gagttatatt tcagtcttaa atatgaaggc ctaacacagt
    10321 ttgagtttaa taaagctttt caaaattata agcgaaatca atattacagt gaagagttgc
    10381 gcgaatatgt tgaagagtac gtagctaatg tagaagaaaa atataacgaa gctttgagta
    10441 gagatgacga tgtaatagct ttatttgaaa caaaattgga tttaggtaaa tacgtccctg
    10501 aatcttttgg tactggtgat gtcattatat tttcaggtgg tgtacttgaa attattgacc
    10561 ttaaatacgg taaaggcatt gaagtttcag ctatagataa tcctcaactt agattatatg
    10621 gcttgggcgc atatgaactg cttagtttaa tgtatgacat tcatacagtt cgcatgacta
    10681 tcatacaacc acgaatagat aacttttcta ctgaagagtt accaatatca agattacttc
    10741 aatggggaac cgattttgtt aaaccattag ccagacttgc ttataacggt gaaggtgagt
    10801 ttaaagcagg tagtcattgt agattctgta agataaagca ttcatgtaga acacgtgcag
    10861 aatacatgca aaatgtgcct caaaagccac cacatttgtt gagtgatgaa gagattgcag
    10921 aacttttata taaactgcct gacatcaaaa aatgggctga tgaagtagaa aaatatgcac
    10981 tagatcaagc gaaagaaaat gataaaaact atcctggttg gaagcttgta gaaggtcgct
    11041 cgcgaagaat gataactgat acaaatgcaa cgcttgaaaa gttagttgaa gcgggttata
    11101 aacctgaaga tattacagaa accaagttac ttagcattac gaatttagaa aaattaatcg
    11161 gcaaaaaagc attttctaaa attgcagaag gctttataga aaagccacaa ggtaaattaa
    11221 cacttgctac cgagtctgat aaacgaccag ctataaagca atctgctgaa gatgattttg
    11281 acaaactata aaaattaaaa aggacggtat ataaacatga aagcacaatt aaaaaatgaa
    11341 actaaagtga ttacaggaaa agtaagagca tcatatgcac atatttttga accgcacagt
    11401 atggatgaaa gcacaccaaa aaaatattcg gtttcactta ttattcctaa agaagacacc
    11461 caaatggtcg agattatcga aaaagcaatt gagaatgcaa aagaagcatt taaaggtaaa
    11521 tggaacggca agatacctaa taacttgaaa acgccattac gtgatggaga cattgatcgt
    11581 gaagatgatc cgaattatga aaatgcgtat tttattaatg ctacaagtca aaatgcacca
    11641 ggtgtagtta gtcctgccat ggttcgtttg aacgaacctg gttcagttgt tagcggagac
    11701 tatatcagag ctgtaatcaa tttctatggt tataacgtaa atggaaataa aggaattgca
    11761 gcagggctca acaacattca acttgtagaa aaaggcgaac ctcttggcgg tgcaagtgca
    11821 gcagaagatg atttcgatga attagacact gatgatgagg atttcttata agtcaatagg
    11881 tggggttttt agccccactt taattttaaa gaaattgagg tgtcaagaat ttgaaattta
    11941 tgaatataga tattgaaaca tatagcagta acgatatttc gaaatgtggt gtctataaat
    12001 acacagaagc tgaagatttc gaaatcttaa ttatagctta ttcaatagat ggtggagcga
    12061 ttagtgcgat tgacatgact aaagtagata ctgagccttt ccacgctgat tatgagacgt
    12121 ttaaaattgc tctatttgac cctgctgtaa aaaagtatgc attcaatgct aatttcgaaa
    12181 gaacttgtct tgctaaatat tttaataaac agatgccacc tgaagaatgg atttgcacaa
    12241 tggttaattc aatgcgtatt ggcttacctg cttcgcttga taaagttgga gaagttttaa
    12301 gactacaaaa ccaaaaagat aaagcaggta aaaatttaat tcgttatttc tctatacctt
    12361 gtaaaccaac aaaagttaat ggaggaagaa caagaaactt gcctgaacat gatcttgaaa
    12421 aatggcaaca atttatagat tactgtattc gagatgtaga agtagaaatg acaattgcta
    12481 ataaaattaa agactttcca gtaactgtaa ttgaacaagc atattgggtt tttgaccaac
    12541 atataaacga cagaggtatt aagctttcta aatcattgat gttaggagct aatgtgctcg
    12601 ataagcagag taaagaagaa ttgcttaaac aagctaaaca tataacaggt ttagaaaatc
    12661 ctaatagtcc tacacagtta ttggcttggt taaaggatga acaaggatta gatataccta
    12721 atttacaaaa gaaaacggtt caggattact taaaagtagc aacaggaaaa gctaaaaaaa
    12781 tgctagaaat tagattgcaa atgtctaaaa ccagtgtgaa aaaatacaac aaaatgcatg
    12841 acatgatgtg cagtgatgaa cgggtaagag gtctgtttca attctacggt gccggtactg
    12901 gaagatgggc aggtagaggt gtacaacttc agaatttaac aaagcattat atttcagata
    12961 ctgaattaga aatagcaaga gatcttatta aagaacaacg ttttgacgat ttagatttat
    13021 tactcaatgt tcatcctcaa gacttattaa gtcaattagt taggacgaca tttactgctg
    13081 aagaaggtaa tgaactagca gtaagtgatt tttctgcaat agaggcaaga gtcatagcat
    13141 ggtatgcaaa agaacaatgg cgtttagatg tgttcaacac acacggaaag atatatgaag
    13201 catcggcttc tcaaatgttt aatgtaccgg tagaaagcat aactaaaggc gaccctctca
    13261 gacaaaaagg aaaagtgtcc gaattagctt taggctatca aggtggcgct ggagctttaa
    13321 aagcgatggg tgcattggaa atgggcattg aagaaaatga attacaaggt ttagttgata
    13381 gttggcgtaa cgcaaatcct aacatagtta atttttggaa ggcttgccaa gaggctgcaa
    13441 ttaatactgt aaaatcccga aagacgcatc atacacatgg acttagattt tatatgaaaa
    13501 aaggttttct aatgattgaa ctgcctagtg gaagagcttt agcttatcca aaagctttag
    13561 ttggtgaaaa tagttggggt agtcaagttg ttgaatttat ggggttagat cttaaccgta
    13621 aatggtcaaa gttaaaaacg tatggtggga agttagtcga gaatattgtt caagcaactg
    13681 caagggattt acttgcgatt tctatagcaa ggcttgaagc atcaggtttt aaaatagttg
    13741 gccatgtcca tgatgaagta attgtagaaa tacctagagg ttcaaatgga cttaaggaaa
    13801 tcgaaactat catgaataag cctgttgatt gggcaaaagg attgaatttg aatagtgacg
    13861 ggtttacttc tccgttttat atgaaggatt aggagtgtga ttgcatgcaa catcaagctt
    13921 atatcaatgc ttctgttgac attagaattc ctacagaagt cgaaagtgtt aattacaatc
    13981 agattgataa agaaaaagaa aatttggcgg actatttatt taataatcca ggtgaactat
    14041 taaaatataa cgttataaat atcaaggttt tagatttaga ggtggaatga tggctagaag
    14101 aaaagttata agagtgcgta ttaaaggaaa actaatgaca ttgagagaag tttcagaaaa
    14161 atatcatata tctccagaac ttcttagata tagatacaaa cataaaatgc gcggcgatga
    14221 attattgtgt ggaagaaagg actcaaaatc taaagatgaa gttgaatata tgaagagtca
    14281 aataaaagat gaagaaaaag ggagagaaaa aatcagaaaa aaagcgattt tgaaccgata
    14341 ccaacgaaat gtgagagcgg aatatgaaga ggaaagaaag agaagattaa gaccatggct
    14401 ttatgatgga acgcctcaaa aacattcacg tgatccgtac tggttcgatg tcacttataa
    14461 ccaaatgttc aagaaatgga gtgaagcata atgagcataa tcagtaacag aaaagtagat
    14521 atgaatgaaa cacaagacaa tgttaaacaa cctgcgcatt acacatacgg cgacattgaa
    14581 attatagatt tcatcgaaca agttacggca cagtatccac cacaattagc attcgcaata
    14641 ggtaatgcaa tcaaatactt gtctagagca ccgttaaaga atggtcatga ggatttagca
    14701 aaggcgaagt tttacgtcga tagagtgttt gacttgtggg aggggtaacg atggcaacgc
    14761 aaaaacaagt tgattacgta atgtcattac aggagcaact ggaattagaa gactgcgaaa
    14821 aatatacaga cgaacaagtt aaagcaatga gtcataaaga agttagcaat gtgattgaga
    14881 actataagac aagcataagg aatgaagaac tatattacga atgcatgtcg tttggactgc
    14941 ctaattgtta aaaggagtga cgaccatgac agatagcgca cgtaaagaac gcttaaacca
    15001 atttttcggc tctaaaagat atctgtatca ggataacgag cgagtggcac atatccatgt
    15061 agtgaatgac atttattatt ttcatgggca tatcgtgcca ggttggcaag gtgtgaaaaa
    15121 gacatttgat actgctgaag agctcgaaat atatataaag caacatggtt tggaatacga
    15181 ggaacagaag caactaactt tattttagag gagatggaaa tgatgaataa ccgtgaacaa
    15241 atagaacaat ccgttataag tgctagtgcg tataacggca atgacacaga gggattgcta
    15301 aaagagattg aggacgtgta taagaaagcg caagcgtttg atgaaatact tgagggttta
    15361 cctaatgcta tgcaagatgc actcaaagaa gatatttatc ttgatgaagc agtaggtatt
    15421 atggtaagtc aagtggtcta taaatatgag gaggaacagg aaaatgacta acacattaca
    15481 agtaaaacta ttatcagaaa atgctagaat gcccgaacga aatcataaga cggatgcagg
    15541 ttatgacata ttctcagctg aaactgtcgt acttgagcca caagaaaagg cagtgatcaa
    15601 aacagatgta gctgtaagca taccagaggg ctatgtcgga ctattaacta gccgtagtgg
    15661 tgtaagtagt aaaacgcatt tagtgattga aacaggcaag atagacgcgg gatatcatgg
    15721 taatttgggg attaatatca agaatgatgc acaagtatat ttaacaacta acgaacagtg
    15781 ttttgatata caaggagaaa tggaaaattc ttttgtaaat aatgctaaga aaaaaccttt
    15841 tactataaat gattattacg aaatatataa aggcgacaaa ctagctcaat tggttatcgt
    15901 gcctatatgg acacctgaac taaagcaagt ggaggaattc gagagtgttt cagaacgtgg
    15961 agcaaaaggc ttcggaagta gcggagtgta aagacatctt agatcgagtc aaggaggttt
    16021 tggggaagtg acgcaatact tagtcacaac attcaaagat tcaacaggac gtaaacatac
    16081 acacataact aaagctaaga gtaatcaaag gtttacagtt gttgaggcag agagtaaaga
    16141 agaagcgaaa gagaaatatg agtcacaaaa tacacctatt gtttactaca ctaataattc
    16201 taaagtgacc ttattcgaaa gacctagtga agaagtatta ggttctttgt tcgaaaagaa
    16261 ataaaatcat taaagagggg agataataat gtttaataca cctaaaatga aattaccaga
    16321 aaagcacacc gaggtattta agacgtataa aaatggaacg ccagaagaaa aagctgagat
    16381 tgaaggctgt tttattaaaa ctgttaaaga tgaagatagt gaattttaca gccctatgtt
    16441 agccagtcta aatgaacaac agttaaagag tatgttgaga caggtacttt ttttgattga
    16501 tacaggagat gacaatgatg attaaaaaac ttaaaaatat ggattggttc gatatcttta
    16561 ttgctggaat actgcgatta ttcggcgtaa tcgcactgat gcttgttgtc atatcgccta
    16621 tctatacagt ggctagttac caaaacaaag aagtacatca agggacaatt acagataaat
    16681 ataacaagag acaagataaa gaagacaagt tctatattgt attagacaac aagcaagtca
    16741 ttgaaaactc tgacttatta ttcaaaaaga aatttgatag cgcagacata caagctaggt
    16801 taaaagtagg cgataaagta aaagttaaaa cgattggtta tagaatacac tttttaaatt
    16861 tatatccagt cttatacgaa gtgaagaagg taggtaaatg atggttaaac aaatattaag
    16921 actattattc ttattagcaa tgtatgagct aggtaagtat gtaactgaac aagtatatat
    16981 tatgatgacg gctaataatg atgtagaggc agcaagtgac tttgaaaaaa tcagagctga
    17041 agtttcatgg taatagctat tatcattttt gaattaatta tattaatgtg tttagcaata
    17101 gcactggagg tgttgtaaat atgtggattg tcatttcaat tgttttatct atatttttat
    17161 tgatcttgtt aagtagcatt tctcataaga tgaaaaccat agaagcattg gagtatatga
    17221 atgcttatct tttcaagcag ttagtaaaaa ataatggtgt tgaaggttta gaagattatg
    17281 aaaatgaagt tgaacgaatt agaaaaagat ttaaaagcta aagagaggcg ttggcttctc
    17341 tgttctatct aaaataatga aaggagccga acatgttaga caaagtcact caaatagaaa
    17401 caattaaata tgatcgtgat gtttcatatt cttatgctgc tagtcgttta tctacacatt
    17461 ggactaatca caatatggct tggtctgact ttatgcagaa gctagcacaa acagttagaa
    17521 ctaaagaaga tttaactgag tacaataaaa tgtctaagtc tgaacaagcc gatataaaag
    17581 atgttggtgg atttgtcggc ggatatttaa aagaagggaa acggcgtgct ggtcaagtca
    17641 tgaatcgttc aatgctaaca cttgatatcg attatgctgc tcaagatatg accgacatat
    17701 tatctatgtt ttatgatttt gcatactgtt tatattcaac acataagcat agagagataa
    17761 gtccaagact gcgtttagtg attcctttaa aacggaatgt aaatgcagat gagtatgaag
    17821 ctattggacg taaagtggca gatatcgttg gcatggatta cttcgatgat acaacttatc
    17881 aaccacatag gttaatgtat tggccttcaa ctagcaatga tgcagaattt ttctttacct
    17941 atgaagattt acctttgtta gatccagata aaatattaaa tgaatatgtt gattggactg
    18001 acacattaga atggccaacg tcttcaaagg aagagagtaa gactaaaaga ttagcagata
    18061 agcaaggtga cccagaagaa aagccgggaa ttgttggcgc attttgtaga gcctatacga
    18121 tagaagaagc tatatcaact tttattcctg acttatacga aaaacattct actaaccgtt
    18181 atacctatca tgaaggttca actgcaggtg gattggtgtt atacgaaaat aacaagtttg
    18241 cctattctca tcataatacg gatcccgtta gcggtatgct tgtgaacagt tttgatttag
    18301 tacgcataca cttatatggt gctcaagatg aagacgctaa aacagatact ccggttaatc
    18361 gactacctag ttataaagca atgcagcaaa gagcgcaaaa tgatgaagtt gttaaaaagc
    18421 aattaattaa cgacaaaatg tctgatgcaa tgcaggattt cgatgaaata gtaaatagcg
    18481 atgatgcatg gtctgagacg ttagaaatta cttcgaaagg tactttcaaa gctagtatcc
    18541 caaatataga aattatattg cgtaatgatc caaatttaaa aggaaaaata gcatttaatg
    18601 aatttacaaa acaaattgaa tgcttaggga aagtgccatg gaatactaat tttaagacac
    18661 gtcaatggca agacggtgat gatagcagtt taagaagtta tatcgaaaag atttatgaca
    18721 tacaccattc aggtaaaaca aaagatgcca ttataagcgt agcaatgcaa aatgcttatc
    18781 atccagtaag ggattatcta aataaaatat cgtgggatgg acataaacgt cttgaaaagt
    18841 tatttatcaa atacttaggt gttgaagata ctgaagtgaa tagaacaact accaaaaaag
    18901 cattgactgc tggaattgct cgagtaatgg agcctggatg taaatttgac tatatgctta
    18961 cactttatgg tcctcaaggt gtaggtaaat ctgctttgct aaaaaaatta ggtggtgcat
    19021 ggttttctga cagtttagtt tctgttacag gtaaagaagc ctatgaggcc ttacaaggcg
    19081 tttggctaat ggaaatggca gaacttgcag ctacaagaaa agctgaagtt gaagctatta
    19141 agcatttcat atctaaacaa gttgaccggt ttcgtgttgc ttatggacat tatattgaag
    19201 attttccaag gcaatgtatt ttcattggta caactaataa agttgatttc ttaagagatg
    19261 aaactggtgg aagacgtttt tggccaatga ctgtaaatcc agagagagtt gaagtgaact
    19321 ggtctaaact aaccaaagaa gagatcgacc aaatttgggc agaagctaaa tattattatg
    19381 aacaaggaga agagttattc ctcaaccctg aactagaaga agaaatgcgt tcaatacaaa
    19441 gcaaacatac tgaggaatct ccatatacag gcattattga tgaatatctt aacacaccaa
    19501 ttcctagcaa ttgggatgac ttaactatct ttgaacgaag acgattttat caaggtgatg
    19561 ttgatatgtt accaacagga aatgtagatt acgttgaaag aaataaggtc tgtgcgcttg
    19621 aagtgtttgt tgaatgtttt ggtaaagata agggagatag tagaggatct atggaaatta
    19681 gaaagatttc aaacatctta agacaattag acaattggtc tgtatatgat ggtaataaaa
    19741 gtgggaaaat tcgatttgga aaagattatg gtgtacagat agcttatgta agagatgaaa
    19801 gtttagaaga tttaatataa taaatattga ataaatatac attttagagt gttgtatcag
    19861 atgttgcatc attttttgag tgatgcaaca cgtgagtgta aaaagtaatc gtaggtgttg
    19921 catcattttt agtgatgcaa cattgatgca acaaatgata caacacctct ttcccttctc
    19981 gctgtaaggt tcaaccctgt ttgtttccaa tgttgcatca aattcactat aaagtttaaa
    20041 aagtagtgtt agggagtaaa gaggtatagg ggtaaccttc taacagctat ttttaaaagt
    20101 ttggcaagaa ttgatgcaac atcggaacac aaatataaat tttgtataca aggtgaatat
    20161 atgaaagaat cgacattaga aaaatattta gtgaaagaga taacaaagct aaacggttta
    20221 tgtttaaaat gggttgcacc tggaacaaga ggtgtgccag atagaattat tattatgcca
    20281 gaaggaaaaa catattttgt agaaatgaag caagaaaaag gaaagttgca tcctttacaa
    20341 aaatatgtgc atagacaatt tgaaaataga gatcataaag tatatgtgtt atggaataaa
    20401 gaacaagtaa atacttttat cagaatggta ggtggaacat ttggcgattg acttcaaacc
    20461 acatagctat caaaagtatg caatagataa agtgatagat aatgagaaat acggtctgtt
    20521 tttagatatg ggtctaggga aaacagtatc aacacttaca gcatttagtg aattgcagtt
    20581 gttagacact aaaaaaatgt tagttatagc acctaaacaa gttgctaaag atacatgggt
    20641 tgatgaagtt gataagtgga accatttaaa tcatctgaaa gtgtctttag ttttaggaac
    20701 acctaaagaa agaaatgatg cattaaacac agaggctgat atctatgtaa ccaataaaga
    20761 aaatactaaa tggttatgtg atcaatataa aaaagaatgg ccatttgaca tggttgtgat
    20821 tgatgaactg tctacattta aaagtcctaa gagtcaaagg tttaaatcta ttaaaaagaa
    20881 attaccactc attaatagat ttataggatt aacaggaaca cctagtccaa atagtttaca
    20941 ggatttatgg gctcaagttt atttgataga cagaggtgaa agacttgagt cttcattcag
    21001 tcgttatcga gaaaggtact ttaaaccaac tcatcaagtt agcgaacata tttttaagtg
    21061 ggagctaaga gacggatctg aagaaaagat atataaacaa atagaagata tatgtttaag
    21121 catgaaagcg aaagattatc tggatatgcc tgacagagtt gatactaaac aaacagtagt
    21181 cttatcagaa aaagaaagaa aagtatatga agaattagaa aaaaactata ttttagaatc
    21241 ggaagaagaa ggaacagttg tagctcaaaa tggggcatca ttaagtcaga aactacttca
    21301 actatctaac ggcgcagttt atacagatga ggaagatgta agacttatac atgataagaa
    21361 gttagataag ttagaggaaa ttatagagga gtctcaaggc caatcaatac tattgtttta
    21421 taacttcaaa cacgataaag aaagaatact tcaaaggttt aaggaagcaa ccacattaga
    21481 ggattcaaac tataaagaac gttggaacag tggagacatt aagttgctta tagcacatcc
    21541 agcaagtgca ggacatggat taaacttaca acaaggtggg cacattattg tttggtttgg
    21601 acttacatgg tccttggaat tataccaaca agcaaatgct agattatata gacaaggaca
    21661 aaatcatacg actattattc atcacatcat gaccgataac acaatagatc aaagagtata
    21721 taaagcttta caaaataaag aactaacgca agaagaattg atgaaagcta ttaaagcaag
    21781 aatagctaag cataagtaat ggaggtataa gatgggaaag gcatcatatg atattaagcc
    21841 aggaacattt aaatatattg aatcagaaat atataattta aatgagaaca agaaagagat
    21901 aaatagattg agaatggaga tacttaaccc aacgaaagaa ctagacacca acattgtgta
    21961 tggaccgtta caaaaaggag agccagttag aacaactgag ttaatggcga caaggttatt
    22021 gactaataag atgttacgta acttagaaga gatggttgaa gcagttgaaa gtgagtactt
    22081 aaagttacct gaagatcata agaaagtaat aaggttaaag tattggaata aagataagaa
    22141 gctaaagata gaacaaatag gggatgcttg tcacatgcat cgcaatacag ttactacaat
    22201 acgaaagaac tttgttaaag cgatagcgta tcatgcaggt atcaaataac attgtgcaaa
    22261 gattgtgcaa aaggcctaca aatctgtagt aatatgatag tatcggaaag atgtataaag
    22321 ttatctgaaa gttatacgac ataaatacat gaggcgcatc gctaagcggt gtgtcttttg
    22381 ttatgcaatc aaagaggtgt aagagatgac caagcataat aacatttata agcatggtcg
    22441 taagtcatat caatacgatt ggttctatca ttcaaaagca tggaagaagt taagagagat
    22501 agcattagat agagataatt atctttgtca aatgtgttta cgcgaagata ttataacaga
    22561 tgcaaagatt gtgcatcaca ttatttatgt tgatgaagat tttaacaaag ctttagactt
    22621 agataatcta atgtcagttt gttatagctg tcataacaaa attcatgcaa atgataatga
    22681 caaaagtaat cttaagaaaa ttagagttct aaaaatttaa ataaaaaaat tatttaaata
    22741 aaattttatg cccccctgcc catcggctta aaatgttttt tcgccgggta ccggagaggc
    22801 ccaaacgcta gcaacgcgga taaatttttc atgaaagggg gtctttatat gaagttaaca
    22861 aaaaaacagc taaaagaata tatagaagat tacaaaaaat ctgatgacat attaattaat
    22921 ttgtatatag aaacatatga attttattgt cggttaagag atgaacttaa aaatagtgat
    22981 ttaatgatag agcatacaaa caaggctggt gcgagcaata ttattaagaa tccattaagc
    23041 atagaactga caaaaacagt tcaaacacta aataacttac tcaagtctat gggtttaact
    23101 gcagcacaaa gaaaaaagat agttcaagaa gaaggtggat tcggtgacta ttaaagtttt
    23161 aaatgaacct tcaccaaaac tattaacaac atggtatgca gagcaagtca ctcaagggaa
    23221 aataaaaaca agcaaatatg ttagaaaaga atgtgagaga catcttagat atctagaaaa
    23281 tggaggtaaa tgggtatttg atgaagaatt agcgcatcgt cctattcgat tcatagaaaa
    23341 gttttgtaaa ccttccaaag gatctaaacg tcaacttgta ttacaaccat ggcaacattt
    23401 tattattggc agtttgtttg gttgggttca taaagaaaca aaactgcgca ggtttaaaga
    23461 agctttgata tttatggggc gaaaaaatgg taaaacaact actatatctg gtgttgctaa
    23521 ctatgctgtt tctcaagatg gagaaaacgg cgctgaaatc catcttttag caaacgtaat
    23581 gaaacaagct agaatattat tcgatgaatc taaggcgatg ataaaagcta gcccaaagct
    23641 tgataaaaat ttcagaacat taagagatga aatccattat gacgcaacga tatcaaaaat
    23701 tatgccccaa gcatcagata gcgataagtt agatggattg aatacacaca tggggatttt
    23761 tgatgaaatt catgaattta aagactataa attgatttca gttataaaaa actcaagagc
    23821 tgcaaggtta caacctcttc tcatctacat tacgacagca gggtatcaat tagatggtcc
    23881 gcttgttgat atggtagaag cgggaagaga caccttagat caaatcatag aagacgaaag
    23941 aactttttat tatttagcat ctttggatga tgacgatgat attaatgatt cgtcgaactg
    24001 gataaaagca aatcccaact taggtgtctc tataaattta gatgagatga aagaagagtg
    24061 ggaaaaagct aagagaacac cagctgaacg tggagatttt ataaccaaaa ggtttaatat
    24121 ctttgctaat aatgacgaga tgagttttat tgattaccca acactccaaa aaaataatga
    24181 aattgtttct ttagaagagc tggaaggcag accatgcacg attggttatg atttatcaga
    24241 aacagaggac tttacagccg cgtgtgctac ttttgcgtta gataatggta aagttgcagt
    24301 tttatcgcat tcatggattc ctaagcacaa agttgaatat tctaacgaaa aaatacccta
    24361 tagagaatgg gaagaagatg gcttattaac agtgcaagat aagccttata ttgactacca
    24421 agatgtttta aattggataa ttaagatgaa tgagcattat gtagtagaaa aaattactta
    24481 tgatagagcg aacgcattca aactaaatca agagttaaaa aattacgggt ttgaaacgga
    24541 agaaacaaga caaggagctt tgaccttgag ccctgcattg aaggatttaa aagaaatgtt
    24601 tttagatggg aaaataatat ttaataataa tcctttaatg aaatggtata tcaataatgt
    24661 tcagttgaaa ctagacagaa acggaaactg gttgccgtct aagcaaagca gatatcgtaa
    24721 aatagatggc tttgcagcat ttttaaacac atatacagat attatgaata aagttgtttc
    24781 tgacaagggt gaaggaaaca tagaatttat tagtattaaa gatataatgc gttaaggagg
    24841 tgaatgttat cgcaaaagag aatattgtca cacgcataaa gaaaaaattg atagacaatt
    24901 ggattgatca gtcagcttct aagctttatg actttagccc atggaaaaat aaatcttttt
    24961 ggggtgtaat caataatacg cttgaaacta atgaaacgat attttcagct attacaaagt
    25021 tatctaattc gatggctagt ttgcccttga aaatgtatga agattataaa gtagttaata
    25081 cagaagtatc tgatttactt acagtgtcac cgaataattc tctgagcagt tttgatttta
    25141 ttaatcaaat tgaaacaatc agaaatgaaa aaggtaatgc atatgtgcta attgaacgag
    25201 acatctatca tcaaccatca aagcttttct tattaaatcc agatgttgtt gaaatgttaa
    25261 ttgaaaacca atcacgtgaa ctttattatt ccattcatgc tgcaactgga aataaattga
    25321 ttgttcataa tatggacatg ttgcatttta aacacatcgt ggcatctaat atggtgcaag
    25381 gcattagtcc gattgatgtg ttgaagaata caactgattt tgataatgca gtaagaacct
    25441 ttaatcttac agaaatgcaa aaacctgatt ctttcatgct taaatatggt tccaatgtag
    25501 gtaaagaaaa aaggcagcaa gtgttagaag atttcaaaca gtactatgaa gaaaacggtg
    25561 gaatattatt ccaagagcct ggtgttgaaa tcgaaccgtt acctaaaaaa tatgtctctg
    25621 aagatatagt ggcaagcgag aatttaacaa gagaaagagt agctaacgtt tttcaattgc
    25681 cctcagtatt cttaaatgca agatcaaata caaatttcgc gaaaaatgaa gagttaaaca
    25741 gattttactt gcagcatacc ttattgccaa tcgtcaaaca gtatgaagaa gaatttaatc
    25801 ggaaactact tactaaaaca gacagagaaa aaaataggta ttttaaattt aacgttaaat
    25861 cttatttaag ggctgatagt gcaacacaag cagaagtgta ctttaaagca gttcgtagtg
    25921 gttactacac tataaatgac attagagagt gggaagattt accaccagtt gaaggtggag
    25981 ataagccgct aataagcggt gatttatacc caattgacac gccacttgaa ttaagaaaat
    26041 ctttgaaagg tggtgataaa aatgtcaatg aaagctaagt attttcaaat gaaaagaaaa
    26101 tcaaaaagta aaggtgaaat atttatttat ggtgatattg taagtgataa atggtttgaa
    26161 agtgatgtaa ctgctacaga tttcaaaaat aaactagatg aactaggaga catcagtgaa
    26221 atagatgttc atataaattc atctggaggc agtgtatttg aagggcatgc aatatacaat
    26281 atgctaaaaa tgcatcctgc aaaaattaat atctatgtcg atgccttagc ggcatcaatt
    26341 gctagtgtta tcgctatgag tggtgacact atttttatgc acaaaaatag ttttttaatg
    26401 attcataatt catgggttat gactgtaggt aatgcagaag aattaagaaa gacagcggat
    26461 ttacttgaaa aaacagatgc tgttagtaat tcagcttatt tagataaagc aaaagattta
    26521 gatcaagaac acttaaaaca gatgttagat gcagaaactt ggcttactgc agaagaagcc
    26581 ttgtctttcg gcttgataga tgaaatttta ggagctaatg aaatagctgc tagtatctct
    26641 aaagagcaat ataagcgttt cgagaacgtc ccagaagatt taaagaaaga tgtagacaaa
    26701 atcactaaaa ttgatgatgt agatacatct gaattggttg aaacacctaa agaaagtatg
    26761 tcactagaag aaaaagaaaa aagagaaaaa attaaacgcg aatgcgaaat tttaaaaatg
    26821 acaatgaatt attaggagga aatgaaatgc cgacattata tgaattaaaa caatccttag
    26881 gtatgattgg acaacaatta aaaaataaaa atgatgaatt gagtcagaaa gcaacagatc
    26941 caaatattga tatggaagac atcaaacaac tagaaacaga aaaagcaggt ttacaacaaa
    27001 gatttaacat tgttgaaaga caagtgcaag acattgaaga gaaagaaaaa gcgaaagtta
    27061 aagataaagg agaagcttat caatctttaa gtgataatga gaagatggtt aaagctaagg
    27121 cagagtttta tcgtcacgcg attttaccaa atgaatttga aaaaccttca atggaggcac
    27181 aacgtttatt acacgcttta ccaacaggaa atgattcagg tggagataag ctcttaccaa
    27241 aaacactttc taaagaaatt gtttcagaac catttgctaa aaaccaatta cgtgaaaaag
    27301 ctcgtctaac taacattaaa ggtttagaga ttccaagagt ttcatacact ttagacgatg
    27361 atgatttcat tacagacgta gaaacagcaa aagaattaaa agcaaaaggt gatacagtca
    27421 agttcactac taataaattc aaagtatttg ctgcaatttc agatactgta attcatggat
    27481 cagatgtaga tttagtaaac tgggttgaaa acgcactaca atcaggatta gcagctaaag
    27541 agcgtaaaga tgccttagca gtaagtccta aatctggatt agaacacatg tcattttata
    27601 atggatctgt taaagaagtt gagggagcag acatgtatga tgctattatt aacgctttag
    27661 cagatttaca tgaagattat cgtgataacg caacaattta tatgcgatat gcagattatg
    27721 tcaaaattat tagtgttctt tcaaatggaa caacaaattt ctttgacaca ccagcagaaa
    27781 aagtatttgg caaaccagta gtatttacag atgcagcagt taaacctatt gtgggagatt
    27841 tcaattattt tggaattaac tatgatggaa caacttatga cactgataaa gatgttagaa
    27901 aaggcgaata tttgtttgta ttaacagcat ggtatgatca gcaacgtaca ttagacagtg
    27961 cattcagaat tgcaaaagca aaagaaaata caggtccatt acccagctaa gccccaaaag
    28021 gttaatgtaa cagctaaggc taaatcagct gtaatatcag ccgaataggg gtgatgaaat
    28081 gagtttagaa gaaattaaat tgtggttgag aattgactat aatttcgaaa atgatttaat
    28141 tgaaggtctc attcaatcgg ctaagtctga attactatta agtggggttc cagattatga
    28201 caaagatgac ttggaatacc cgcttttttg tacagcgatt aaatatatca ttgcaagaga
    28261 ttatgaaagt cgtggatact caaatgacca atctagaagc aaggtgttta atgaaaaagg
    28321 attgcaaaaa atgattttga aattaaaaaa gtggtaggtg atttttaaat ggaatttaat
    28381 gaatttaaag atcgcgcgta tttttttcaa tatataaaca aaggaccata tccagatgaa
    28441 gaggaaaaaa tgaaattgta tagttgcttt tgtaaaattt ataatccttc tatgaaagat
    28501 agagaaattt taaaagcgac tgaatcaaaa tcaggattaa ccataattgt caggtcttct
    28561 aaaactgaat atctaccaca aacaaatcac ttagttaaaa ttgacagtgc attatattcc
    28621 gataaattat tcaacattgt agaaataaga attgatacac cagatattgg ctataataca
    28681 gtggttttat cagaaaaatg agtgtagaaa ttaaagggat acctgaagtg ttgaataaat
    28741 tagaatcggt atacggtaaa caagcaatgc aggctaagag tgataaagct ttaaatgaag
    28801 catctgaatt ttttataaag gctttaaaga aagagttcga gagctttaaa gatacgggtg
    28861 ccagtataga agaaatgact aaatctaagc cttatacaaa agttggcagt caagaaaggg
    28921 ctgttttaat tgaatgggta ggccctatga atcgcaaaaa cattattcac ttgaatgaac
    28981 atggttatac aagagatgga aaaaaatata caccaagagg ttttggagtt attgcaaaaa
    29041 cattagctgc tagcgaacgt aagtatagag aaattataaa aaaggagttg gccagataaa
    29101 tgaatatatt aaacaccata aaaggaattt tattatctga tgcagagctc aaaacacata
    29161 taaattctag aatatactat tacaaagtca ctgaaaacgc tgaaacttcc aaaccctttg
    29221 ttgttattac acctgtttat gatttgcctt cagactttat gtctgataaa tatctcagtg
    29281 aagaatactt aattcaaata gatgtagaat cttcaaataa tcagaaaaca attgatataa
    29341 caaaacgaat aagatacctg ttatatcaac aaaatttaat tcaagcatct agtcagttag
    29401 atgcttattt tgaagaaact aaacgttatg tgatgtcgag acgatatcaa ggcataccca
    29461 aaaatatata ttataaaaat cagcgcatcg aataggtgtg ctttttaatt tttaaggagg
    29521 aaataagcaa tggcagaagg acaaggttct tataaagtag gttttaaaag attatacgtt
    29581 ggagttttta acccagaagc aacaaaagta gttaaacgca tgacatggga agatgaaaaa
    29641 ggtggtacag ttgacctaaa tatcacaggt ttagcaccag atttagtaga tatgtttgca
    29701 tctaacaaac gtgtatggat gaaaaaacaa ggtactaatg aagttaagtc tgacatgagt
    29761 attttcaata ttccaagtga tgatttaaac acagttattg gacgtactaa agataaaaat
    29821 ggtacatctt gggtaggaga gaatacaaga gcaccgtatg taacagtaat tggcgaatcg
    29881 gaagatggtt taacaggtca gccggtatat gtagccttac ttaaaggtac ttttagttta
    29941 gattcaattg aatttaaaac acgaggtgaa aaagcagaag ccccagaacc tacaaaatta
    30001 acaggtgact ggatgaatag aaaagttgat gttgatggaa cgtcacaagg tattgtatac
    30061 ggttatcatg aaggtaaaga aggagaagca gaattcttca aaaaagtatt cgttggatac
    30121 acggacagtg aagatcattc agaggattct gcaggttcgt tacccagcta atccccaaaa
    30181 tgttgaagta gcagttaatt caaaatctgc aacagtttca gcagaatagg ggctttcaaa
    30241 ataaatcaaa ggagaataat ttatgactaa aactttaaag gtttataaag gagacgacgt
    30301 cgtagcttct gaacaaggtg aaggcaaagt gtcagtaact ttatctaatt tagaagcgga
    30361 tacaacttat ccaaaaggta cttaccaagt ggcatgggaa gaaaatggta aagaatctag
    30421 taaagttgat gtacctcaat tcaaaaccaa tccaattcta gtctcaggcg tatcatttac
    30481 accagaaact aaatcaatta tggtaaatac cgatgacaat gttgagccaa acattgcacc
    30541 aagcacagca acgaataaaa tattgaaata tacaagtgaa catccagaat ttgttactgt
    30601 agatgaaaat acaggagcaa ttcacggtgt agctgaaggt acttcagtaa tcactgctat
    30661 gtctactgat ggaagcgata agtcaggaca aatttcagtg acagtaacaa acggataggg
    30721 atttaaggcg cagtatatct gcgtcttttt tatttgaata aaaggagcta atacaatgat
    30781 taaatttgaa attaaagatc gtaaaacagg aaaaacagag agctatacaa aagaagatgt
    30841 aacaatgggc gaagcagaaa aatgctatga gtatttagaa ttagtaaatc aagagaataa
    30901 aaaagaagca cctaacgcaa caaaaatgag acaaaaagag cgacagttat tagtagattt
    30961 atttaaagat gaaggattga ctgaagaaga tgttctgaac aagatgagta ctaaaactta
    31021 tacaaaagcc ttacaagata tatttcgaga aatcaatggt gaagatgaag aagattcaga
    31081 aactgaacca gaagagatgg gaaagacaga agaacaatct caataaaaga cattttatcg
    31141 aacattaaga aaatacaacg tttctgtatg gagcagtatg ggtggacatt aactgaagtc
    31201 agaaaacagc cgtatgtaaa acttttagaa atacttaatg aagagaataa agaagagact
    31261 gaagaaaaac aaagtgaaca aaaagtcatt acaggtacgg atttaagaaa actttttgga
    31321 agctagaaag gaggttaata tgaatgaaaa agtagaaggc atgaccttgg agctgaaatt
    31381 agaccattta ggtgtccaag aaggcatgaa aggtttaaag cgacaattag gtgttgttaa
    31441 tagtgaaatg aaagctaatc tgtcagcatt tgataagtct gaaaaatcaa tggaaaaata
    31501 tcaggcgaga attaaggggt taaatgatag gcttaaagtt caaaaaaaga tgtattctca
    31561 agtagaagat gagcttaaac aagttaacgc taattaccaa aaagctaaat ccagtgtaaa
    31621 agatgttgag aaagcatatt taaagttagt agaagccaat aaaaaagaaa aattagctct
    31681 tgataaatct aaagaagcct taaaatcatc gaatacagaa cttaaaaaag ctgaaaatca
    31741 atataaacgt acaaatcaac gtaaacaaga tgcgtatcaa aaacttaaac agttgagaga
    31801 tgcagaacaa aagcttaaga atagtaacca agctactact gcacaactaa aaagagcaag
    31861 tgacgcagta cagaagcagt ccgctaagca taaagcactt gttgaacaat ataaacaaga
    31921 aggcaatcaa gttcaaaaac taaaagtgca aaatgacaat ctttcaaaat caaatgataa
    31981 aattgaaagt tcttacgcta aaactaatac taaattaaag caaacagaaa aagaatttaa
    32041 tgatttaaac aatactatta agaatcatag cgctaatgtc gcaaaagctg aaacagctgt
    32101 taataaagaa aaagctgctt taaataattt ggagcgttca atagataaag cttcatccga
    32161 aatgaagact tttaacaaag aacaaatgat agctcaaagt catttcggta aacttgcaag
    32221 tcaagcggat gtcatgtcaa agaaatttag ttctattgga gacaaaatga cttccctggg
    32281 acgtacaatg acgatgggcg tatctacacc aattacttta gggttaggtg cagcattaaa
    32341 aacaagtgca gactttgaag gccaaatgtc tcgagttgga gcgattgcgc aagcaagcag
    32401 taaagacttg aaaagcatgt ctaatcaagc agttgactta ggagctaaaa ccagtaaaag
    32461 tgctaacgaa gttgctaaag gtatggaaga attggcagct ttaggcttta atgccaaaca
    32521 aacaatggag gctatgccag gtgttatcag tgcagcagaa gcaagtggtg cagaaatggc
    32581 tacaactgca actgtaatgg cttcagcgat taactctttc ggtttaaaag catctgatgc
    32641 aaatcatgtt gctgatttac ttgcgagatc agcaaatgat agtgctgcag atattcagta
    32701 catgggagat gcattgaagt atgctggtac tcctgcaaaa gcattaggag tttcaataga
    32761 ggacacttcc gcagcaattg aagttttatc taactcaggt ttagagggtt ctcaagcagg
    32821 tactgcccta agagcttcat ttatcaggct agctaatcca agtaaaaata cagctaagga
    32881 aatgaaaaaa ttaggtattc atttgtctga tgctaaaggt caatttgttg gcatgggtga
    32941 attgattaga cagttccaag ataatatgaa aggcatgacg agagaacaaa aactagctac
    33001 agtggctaca atagttggta ctgaagcagc aagtggattt ttagccttga ttgaagcggg
    33061 accagataaa attaatagct atagtaaatc cttaaagaat tccaatggcg aaagtaaaaa
    33121 agcagcagat ttgatgaaag ataatctcaa aggcgctctg gaacaattag gtggcgcttt
    33181 tgaatcatta gcaatcgaag tcggtaaaga tttaacgcct atgattagag caggagcgga
    33241 aggtttaaca aaattagttg atggatttac acatctccct ggttgggtta gaaaagcttc
    33301 agtaggatta gcactttttg gtgcagcaat tggacctgca gttcttgctg gagggttatt
    33361 aatacgtaca gttggaagtg ctgctaaagg atatgcgtca ttaaatagac gtattgctga
    33421 aaatacaatc ctttcaaata ctaattcaaa agcaatgaaa tctttaggtc ttcaaacatt
    33481 atttcttggt tctacaacag gaaaaacgtc aaaaggcttt aaagggttag ccggagctat
    33541 gatgtttaat ttaaaaccta taaatgtttt gaaaaattct gcaaagctag caattttacc
    33601 gttcaaactt ttgaaaaacg gtttaggatt agctgcaaaa tctttatttg cagtaagtgg
    33661 aggcgcaaga tttgcgggtg tagccttaag gtttttaaca ggacctatag gtgctacaat
    33721 aactgctatt acaattgcgt ataaagtttt taaaaccgca tatgatcgtg tggaatggtt
    33781 cagaaacggt attaacggtt taggagaaac tataaagttt tttggtggta aaattattgg
    33841 cggcgctgtt agaaagctag gagagtttaa aaactatctt ggaagtatcg gcaaaagctt
    33901 caaagaaaag ttttcaaagg atatgaaaga tggttataaa tctttgagtg acgatgacct
    33961 tctgaaagta ggagtcaaca agtttaaagg atttatgcaa accatgggca cagcttctaa
    34021 aaaagcgtct gatactgtaa aagtgttagg gaaaggtgtt tcaaaagaaa cagaaaaagc
    34081 tttagaaaaa tacgtacact attctgaaga gaacaacaga atcatggaaa aagtacgttt
    34141 aaactcgggt caaataacag aagacaaagc aaaaaaactt ttgaaaattg aagcggattt
    34201 atctaataac cttatagctg aaatagaaaa aagaaataaa aaggaactcg aaaaaactca
    34261 agaacttatt gataagtata gtgcgttcga tgaacaagaa aagcaaaaca ttttaactag
    34321 aactaaagaa aaaaatgact tgcgaattaa aaaagagcaa gaactcaatc agaaaatcaa
    34381 agaattgaaa gaaaaagctt taagtgatgg tcagatttca gaaaatgaaa gaaaagaaat
    34441 tgaaaagctt gaaaatcaaa gacgtgacat cactgttaaa gaattgagta agactgaaaa
    34501 agagcaagag cgtattttag taagaatgca aagaaacaga aatgcttatt caatagacga
    34561 agcgagcaaa gcaattaaag aagcagaaaa agcaagaaaa gcaagaaaaa aagaagtgga
    34621 taagcagtat gaagatgatg tcattgctat aaaaaataac gtcaaccttt ctaagtctga
    34681 aaaagataaa ttgttagcta ttgctgatca aagacataag gatgaagtaa gaaaggcaaa
    34741 atctaaaaaa gatgctgtag tagacgttgt taaaaagcaa aataaagata ttgataaaga
    34801 aatggattta tccagtggac gtgtatataa aaatactgaa aagtggtgga atggtcttaa
    34861 aagttggtgg tctaacttca gagaagacca aaagaagaaa agtgataaat acgctaaaga
    34921 acaagaagaa acagctcgta gaaacagaga aaatataaag aaatggtttg gaaatgcttg
    34981 ggacggcgta aaaactaaaa ctggtgaagc ctttagtaaa atgggcagaa atgctaatca
    35041 ttttggcggc gaaatgaaaa aaatgtggag tggaatcaaa ggaattccaa gcaaattaag
    35101 ttcaagttgg agctcagcca aaagttctgt aggatatcac actaaggcta tagctaatag
    35161 tactggtaaa tggtttggaa aagcttggca atctgttaaa tcgacaacag gaagtattta
    35221 caatcaaact aagcaaaagt attcagatgc ctcagataaa gcttgggcgc attcaaaatc
    35281 tatttggaga ggcacatcaa aatggtttag caatgcatat aaaagtgcaa agggctggct
    35341 aacggatatg gctaataaat cgcgctcgaa atgggataat atttctagta cagcatggtc
    35401 gaatgcaaaa tccgtttgga aaggcacatc gaaatggttt ggtaactcat acaaatcttt
    35461 aaaaggttgg actggggata tgtattcaag agcccacgat cgttttgatg caatttcaag
    35521 ttcggcatgg tctaacgcta aatcagtatt taatggtttt agaaaatggc tatcaaaaac
    35581 atatgattgg attagagata ttggtaaaga catgggaaga gctgcggctg atttaggtaa
    35641 aaatgttgct aataaagcta ttggcggttt gaatagcatg attggcggta ttaataaaat
    35701 atctaaagcc attactgata aaaatctcat caagccaata cctacattgt ctactggtac
    35761 tttagcagga aagggtgtag ctaccgataa ttcgggagca ttaacgcaac cgacatttgc
    35821 tgtattaaat gatagaggtt ctggaaacgc cccaggtggt ggagttcaag aagtaattca
    35881 cagggctgac ggaacattcc atgcacccca aggacgagat gtggttgttc cactaggagt
    35941 tggagatagt gtaataaatg ccaatgacac tctgaagtta cagcggatgg gggttttgcc
    36001 aaaattccat ggtggtacga aaaagaaaaa atggatggaa caagttactg aaaatcttgg
    36061 taaaaaagca gggggcttcg gttctaaagc taaaaacaca gctcataata tcaaaaaagg
    36121 tgcagaagaa atggttgaag cggcaggcga taaaatcaaa gatggtgcat cttggttagg
    36181 cgataaaatc ggcgatgtgt gggattatgt acaacatcca gggaaactag taaataaagt
    36241 aatgtcaggt ttaaatatta attttggagg cggagctaac gctacagtaa aaattgctaa
    36301 aggcgcgtac tcattgctca aaaagaaatt agtagacaaa gtaaaatcgt ggtttgaaga
    36361 ttttggtggt ggaggcgatg gaagctatct atttgaccat ccaatctggc aaagatttgg
    36421 acgctacaca ggtggactta actttaatgg cggtcgtcac tatggtatcg actttcaaat
    36481 gcctactgga acgaacattt atgctgttaa aggcggtata gctgataaag tatggactga
    36541 ttacggtggc ggtaattcta tacaaattaa gaccggtgct aatgaatgga actggtatat
    36601 gcatttatct aagcaattag caagacaagg ccaacgtatt aaagctggtc aactgatagg
    36661 gaaatccggt gctacaggta atttcgttag aggagcacac ttacatctcc aattgatgag
    36721 aggttcacat ccaggtaatg atacagcagt agatcctatg aaatggttga agtcacttaa
    36781 aggtggcggt ggtaaggttg gcggaagcgg atacgagaat gcaaaaagag ctattctaag
    36841 agcacaatca attttaggtg gacgatatag atctgactat atcactactc agatgttaag
    36901 agtagccaag cgtgaaagta actatcaggc agatgctatt aacaactggg attccaacgc
    36961 aagagcaggt acaccgtcta aaggtatgtt ccaaatgatt gagccttcat ttagagcttt
    37021 tgctaagccc ggacatggaa atatttataa tcctactgat gaggcgattt cagcaatgaa
    37081 atacattgtt gctaagtacg gttggggagg ctttaaacgc gcaggagatt atgcctatgc
    37141 gaatggaggt cttataacta aacatcaaat cgctgaagtg ggagaaggag ataaaccaga
    37201 aatggttatt ccgttgacac gacgcaaaag agcaatgcaa ttaactgaac aggttatgcg
    37261 catcatcggt atggatggca agccaaataa catcactgta aataatgata cttcaacagt
    37321 tgaaaaattg ttgaaacaaa ttgttatgtt aagtgataaa ggaaataaat taacagatgc
    37381 attgattcaa actgtttctt ctcaggataa taacttaggt tctaatgatg caattagagg
    37441 tttagaaaaa atattgtcaa aacaaagtgg gcatagagca aatgcaaata attatatggg
    37501 aggtttgact aattaatgca atcttttgta aaaatcatag atggttacaa ggaagaagta
    37561 ataacagatt ttaatcagct tatattttta gatgcaaggg ctgaaagtcc aaacaccaat
    37621 gataacagtg taactattaa cggagtagat ggtattttac cgggcgcaat tagttttgcg
    37681 cctttttcat tagtattaag gtttggctat gatggtatag atgttataga tttaaattta
    37741 tttgagcatt ggtttagatc tgtgtttaat cgcagacatc cttattatgt tattacttct
    37801 caaatgcctg gtgttaaata tgcagtgaat acagctaatg ttacatctaa tttaaaagat
    37861 ggttcttcaa ctgaaattga agtaagttta aatgtttata aagggtattc tgaatcagtt
    37921 aattggaccg atagcgagtt cttattcgac tctaattgga tgtttgaaaa tggaattcct
    37981 cttgatttca cacctaaata tactcataca tcaaatcaat ttactatttg gaacggttct
    38041 actgatacga taaatccacg attcaagcac gatttgaaaa tattaattaa tttaaatgcg
    38101 agtggaggat ttgaactggt taactataca acaggtgata tttttaagta caacaaaagt
    38161 atagataaaa acactgattt tgttttagat ggtgtgtatg catatcgaga tataaataga
    38221 gtgggaattg atacaaatag aggcattata acattagcgc caggtaaaaa tgaatttaag
    38281 attaaaggag acgtcagtga tattaaaact acatttaagt ttccttttat ttataggtag
    38341 gtgatttaat ggattatcat gatcatttat cagtaatgga ttttaatgaa ttgatttgtg
    38401 aaaatttact agatgtagat tatggttctt ttaaagaata ttatgaactg aatgaagcta
    38461 ggtacatcac ctttacagtt tatagaacta ctcataatag ttttgttttt gatttattga
    38521 tttgtgaaaa cttcataatt tatcatggtg aaaaatatac aattaagcag acagcgccaa
    38581 aggttgaagg tgataaagtt tttattgaag ttacggcata tcacataatg tatgaatttc
    38641 aaaatcactc agtggaatca aataagcttg atgacgacag tagcgaaact ggtaaaacgc
    38701 cagaatactc tttagatgag tacttaagat atggatttgc aaatcaaaaa acttcggtca
    38761 aaatgaccta taaaataatt ggagatttta agcgaaaagt accgattgac gaattaggta
    38821 acaaaaacgg cttagaatac tgtaaagaag cggtagacct gtttggctgt ataatttacc
    38881 caaatgatac agagattggt ttttattctc ctgaaacatt ttatcaaaga agcgagaaag
    38941 tgattcgata tcaatataat actgatactg tatctgcaac tgtcagtaca ttggaattaa
    39001 gaacagctat aaaagttttt ggaaaaaagt atacagctga ggaaaagaaa aattataatc
    39061 ctattagaac aactgacatt aaatattcaa atggttttat aaaagaaggt acttatcgta
    39121 ccgaaacaat tgggtctaaa gctactatta actttgattg caagtatggt aatgaaacag
    39181 ttagatttac aataaaaaag ggctctcaag gtggaatata taagttgatt ttagacggca
    39241 agcaaattaa gcaaatttct tgttttgcta agtcggttca gtctgaaaca atagatttaa
    39301 taaaaaatat tgataaaggc aagcacgttt tagaaatgat atttttagga gaagacccca
    39361 aaaatagaat tgatatatct tcaaataaaa aagctaagcc ttgtatgtat gttggaactg
    39421 aaaaatcaac agtcttaaat ttaattgctg acaactcagg tcgcaatcaa tacaaagcaa
    39481 ttgttgacta cgtcgcagat agtgcaaagc agtttgggat tcgatatgct aatacgcaaa
    39541 caaatgaaga tatcgaaaca caggataagc tgttagaatt tgcaaaaaag caaataaatg
    39601 atactcctaa gactgaatta gatgttaatt atataggtta tgaaaaaata gagccaagag
    39661 atagcgtatt ttttgttcat gaattaatgg gatataacac tgaattaaag gttgttaaac
    39721 ttgataggtc acatccattt gtaaacgcaa tagatgaagt gtctttcagc aatgaaataa
    39781 aagatatggt acaaattcaa caagcactta acagacgagt tattgcacaa gataatagat
    39841 ataactatca agcaaatcgt ataaatcatt tatacactag tactttgaat tctcctttcg
    39901 agacaatgga tatagggagt gtattaatat aatggcaaca gaagaagtta aaatcaaagc
    39961 gctacttgaa aacgataaac agtactttcc agctacacac tggaaagcta taaatgggat
    40021 accttatgca ggcagtagtg atattgatgg attgcctcaa gacggtatca tttcggtaga
    40081 tgataaaaat aaattagata aattaaaaat aggcgaagca ggaattattc aaaatagcat
    40141 tgtacagaaa tccccaaacg gtaaattgtg gaaaataaca gttgacgata gtgggaaact
    40201 tggtacagtg ctattttatt agaaaggaag gtgcattatg gaaaatttgt atttaataaa
    40261 ggatttggga gctttagcag gtcgagatta tagagctaaa gaaattcaaa acctgcaaag
    40321 aatagagcaa tttgcgcttg gcttgacaac agagtttaag ttgcatcaga aagctaaaac
    40381 aatgcaacac ttcgctgagc aaatttatta taatggtaga tcgcaagcag cagtaaacaa
    40441 atctttacaa agtcaaatta acgcacttgt tgtggcacca cgtaataaca gtgctaatga
    40501 gattgttcaa gctcgagtta atgtaaacgg cgaaaccttt gacacattaa aagaacattt
    40561 agacgattgg gaaaccaaaa ctcaaattaa taaagaggaa actataagag aattaaataa
    40621 gaccaaacaa gaaattcttg atatcgagta tcgttttgaa cctgataagc aagagttttt
    40681 atttgtgaca gaacttgcac ctcttacaaa tgcagtaatg caatccttct ggtttgataa
    40741 tagaacaggc atagtataca tgacacaagc tagaaataat ggctatatgc taagtcgtct
    40801 aagacctaat ggtcaattta tagacagctc attgattgta ggtgggggtc atggtacaca
    40861 taacggttat agatatattg atgatgagtt atggatttat agttttatct taaatggtaa
    40921 taatgagaat acattagttc gtttcaagta tacgcctaat gtggaaatta gctatggcaa
    40981 gtatggtatg caagatgtat ttacaggaca cccagaaaaa ccctacatca cccctgtcat
    41041 aaatgaaaaa gaaaataaaa ttctatacag aattgagaga cctagaagtc agtgggaact
    41101 tgaaaactca atgaattata tagagataag aagtttagac gatgttgata aaaatattga
    41161 taaagttttg cataaaatca gtatccctat gagactaaca aacgaaaccc aaccaatgca
    41221 gggtgtgact tttgatgaaa aatacttgta ttggtataca ggagacagta atccaaataa
    41281 tagaaactat ttaacggctt tcgatttaga aacaggagaa gaagcgtatc aggttaatgc
    41341 tgactatggt ggaacactag attcatttcc tggcgaattt gcggaagcag aaggtttgca
    41401 aatatactat gacaaagata gtggtaaaaa agctttgatg ctaggtgtta ctgtcggtgg
    41461 tgatggaaat agaacacatc gtattttcat gattgggcaa agaggtattt tagaaatact
    41521 tcactcaaga ggcgttcctt ttatcatgag tgacacaggt ggtagagtta aacctttacc
    41581 aatgaggcct gataaactta agaatcttgg gatgttaaca gagccaggtc tttactattt
    41641 atacactgat catacagttc aaatcgatga tttcccatta ccaagagaat ggcgtgatgc
    41701 aggttggttc ttagaagtaa aaccaccaca aactggcggt gatgtgattc agatattgac
    41761 gcgtaatagt tatgcaagaa atatgatgac ttttgaaagg gtgctttctg gaagaactgg
    41821 agacatttcg gactggaatt atgtgcctaa aaatagtggt aaatgggaga gagtaccttc
    41881 attcatcaca aaaatgtcag atattaacat agtaggcatg tcgttttatt taactacgga
    41941 tgatacaaaa cgttttacag attttccaac tgaacgtaaa ggggtagctg gttggaactt
    42001 atatgtagaa gcttcaaaca caggtggctt tgttcatagg ctagttcgta atagtgttac
    42061 agcatctgct gagatactat tgaaaaatta tgatagtaaa acaagttcag ggccatggac
    42121 tttacacgaa gggagaatta taagttaatg agtaatttag agaaatctgt agctataaat
    42181 ttagaaaaca cagcgcatta tgaaaatatt tcaaatctag atataacttt tagaacagga
    42241 gagagtgatt cttctgttct tctttttaat atcattaaaa ataatcaacc gttattactg
    42301 agtgaagaaa atatcaaagc acgaatagcg attcgaggta aaggagtaat ggtagttgct
    42361 ccactagaaa tattagatcc atttaaaggt attttaaaat ttcaattacc taatgatgta
    42421 attaaacgag atggaagtta tcaagctcaa gtttcggttg cagaattagg taattcagac
    42481 gtggtagttg tcgagagaac tatcacattt aacgttgaaa aaagtttgtt tagcatgatt
    42541 ccatctgaaa caaaattaca ctatattgtt gaatttcagg aattagaaaa aactattatg
    42601 gatcgtgcga aagcaatgga cgaggctata aaaaatggtg aagattatgc gagtctgatt
    42661 gaaaaagcta aagaaaaagg tctatcagat attcaaatag caaaatcttc aagtatagat
    42721 gaattaaagc aacttgctaa tagccatata tctgatttgg aaaataaagc gcaagcatat
    42781 tcaagaacat tcgatgagca aaagcgatat atggatgaga aacatgaagc cttcaagcag
    42841 tcagtgaata gtggtggttt agtcacaagt ggttctactt caaattggca aaaagctaag
    42901 attactaaag atgatggtaa gataatgcag attactggat ttgattttaa taatccagaa
    42961 caaagaatag gtgattcaac ccaatttatt tatgtttcgc aagctataaa ttatccaaga
    43021 ggtgttagta ctaacggtac tgtcgaatat ttagtagtaa cttcagatta caagcgtatg
    43081 acttatcgac cgaacggtac aaataaagtg tttgttaaaa gaaaagaagc gggttcatgg
    43141 tctgagtggt cagaattagc tattaatgat tacaatacac cttttgaaac tgttcaaagt
    43201 gcccaatcaa aagctaatat ggccgaaagt aacgctaaat tatacgcaga tgacaagttt
    43261 aataaaaggt attcggttat ttttgatgga acagcaaatg gtgtgggctc tacattgtac
    43321 ttaaatgaga gtttagacca atttatttta ttaatttttt atgggacttt tccaggtggt
    43381 gactttacag agtttggcag tccttttgga ggaggaaaga tttcattgaa tccctcaaat
    43441 cttccagatg gtgatggaaa tggtggaggt gtttatgagt ttggattaac taaatctagt
    43501 cgtacatctt taactatatc aaacgatgtc tatttcgact taggaagtca aagaggctct
    43561 ggtgcgaacg caaatagagg gacaattaac aaaattatag gagtgagaaa ataatgcaaa
    43621 tattagttaa caagcgtaat gagataattt catacgctat cattggtggc tttgaagaag
    43681 gtattgatat tgaaaattta ccagaaaatt tctctcaagt ttttagacct aaagccttta
    43741 aatattcaaa tggggaaata gtttttaacg aagattattc agaagaaaaa gatgacttgc
    43801 atcaacagat tgacagtgaa gaacaaaaca cagtcgcttc tgatgacatc ttacgaaaaa
    43861 tggttgctag tatgcagaaa caagttgttc aaagtacaaa gttatcgatg caagttaata
    43921 agcaaaatgc actaatggca aaacaacttg tgacacttaa taaaaaatta gaagaggtta
    43981 aaggagagac tgaaaatgct taaattaatt tcaccaacat tcgaagatat taaaacatgg
    44041 tatcaattga aagaatatag taaagaagat atagcgtggt atgtagatat ggaagttata
    44101 gataaagagg aatatgcaat tattacagga gaaaagtatc cagaaaatct agagtcatag
    44161 gttataatct tatggctttt taatttgaat aaagtgggtg gtgtaatgtt tggatttacc
    44221 aaacgacacg aacaagattg gcgtttaacg cgattagaag aaaatgataa gactatgttt
    44281 gaaaaattcg acagaataga agacagtctg agaacgcaag aaaaaattta tgacaagtta
    44341 gata
//
LOCUS       DAHHOJ010000006        43524 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.006, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000006 DAHHOJ010000000
VERSION     DAHHOJ010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 43524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.006"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..298
                     /locus_tag="NBH32_000265"
     CDS             <1..298
                     /locus_tag="NBH32_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1513904.1"
                     /translation="NIDSSLYSKFIILGNIGVLFNAVSIMIQTLNTKHASITLQANYM
                     TFHTITFIFITILMTIAFGLNGFFWTTLFSNIIKYVILNIIGLKSKFINKKDVD"
     gene            299..1504
                     /gene="cap8L"
                     /locus_tag="NBH32_000266"
     CDS             299..1504
                     /gene="cap8L"
                     /locus_tag="NBH32_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8L"
                     /protein_id="HCD1513905.1"
                     /translation="MSEKKILILCQYFYPEYVSSATLPTQLAEDLIANHINVDVMCGW
                     PYEYSNHKQVSKTEMHRGIRIRRLKYSRFNNKSKVGRIINFFSLFSKFVINIPKMLKY
                     DQILVYSNPPILPLIPDVLHRLLKKKYSFVVYDIAPDNAIKTGATRPGSMIDKLMRYI
                     NRHVYKNAENVIVLGTEMKNYLLNHQISKNADNIHVIPNWYDMRQLQDNRIYNDTFKA
                     YREQYDKILLYSGNMGQLQDMETLISFLKLNKDQPQTLTILCGHGKKFADVKTAIEDH
                     RIENVKMFEFLTGTDYADVLKIADVCIASLIKEGVGLGVPSKNYGYLAAKKPLVLIMD
                     KQSDIVQHVEQYDAGIQIDNGDAHAIYNFINTHSSKELHEMGERAHQLFKDKYTREIN
                     TMKYYNLLK"
     gene            1515..2072
                     /gene="cap8M"
                     /locus_tag="NBH32_000267"
     CDS             1515..2072
                     /gene="cap8M"
                     /locus_tag="NBH32_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8M"
                     /protein_id="HCD1513906.1"
                     /translation="MKRLFDVVSSIYGLVVLSPILLITALLIKMESPGPAIFKQKRPT
                     INNELFNIYKFRSMKIDTPNVATDLMDSTSYITKTGKVIRKTSIDELPQLLNVLKGEM
                     SIVGPRPALYNQYELIEKRTKANVHTIRPGVTGLAQVMGRDDITDDQKVAYDHYYLTH
                     QSMMLDMYIIYKTIKNIVTSEGVHH"
     gene            2072..2959
                     /gene="capN"
                     /locus_tag="NBH32_000268"
     CDS             2072..2959
                     /gene="capN"
                     /locus_tag="NBH32_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     epimerase CapN"
                     /protein_id="HCD1513907.1"
                     /translation="MRKNILITGVHGYIGNALKDKLIEQGHQVDQINVRNQLWKSTSF
                     KDYDVLIHTAALVHNNSPQARLSDYMQVNMLLTKQLAQKAKAEDVKQFIFMSTMAVYG
                     KEGQVGKSDQIDTQTPMNPTTNYGISKKFAEQALQELISDSFKVAIVRPPMIYGAHCP
                     GNFQRLMQLSKRLPIIPNINNQRSALYIKHLTAFIDQLISLEVTGVYHPQDSFYFDTS
                     SVMYEIRRQSHHKTVLINMPSMLNKYFNKLLVFRKLFGNLIYSNTLYENNNALEVIPG
                     KMSLVIADIMDETTTKDKA"
     gene            3013..4275
                     /gene="cap8O"
                     /locus_tag="NBH32_000269"
     CDS             3013..4275
                     /gene="cap8O"
                     /locus_tag="NBH32_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8O"
                     /protein_id="HCD1513908.1"
                     /translation="MKLTVVGLGYIGLPTSIMFAKHGVDVLGVDINQQTIDKLQSGQI
                     SIEEPGLQEVYEEVLSSGKLKVSTTPEASDVFIIAVPTPNNDDQYRSCDISLVMHALD
                     SILPFLEKGNTIIVESTIAPKTMDDFVKPVIENLGFTIGEDIYLVHCPERVLPGKILE
                     ELVHNNRIIGGVTKACIEAGKRVYRTFVQGEMIETDARTAEMSKLMENTYRDVNISLA
                     NELTKICNNLNINVLDVIEMANKHPRVNIHQPGPGVGGHCLAVDPYFIIAKDPENAKL
                     IQTGREINNSMPAYVVDTTKQIIKALSGNKVTVFGLTYKGDVDDIRESPAFDIYELLN
                     QEPDIEVCAYDPHVALDFVEHDMSHAVKDASLVLILSDHSEFKNLSDSHFDKMKHKVI
                     FDTKNVVKSSFEDVSYYNYGNIFNFIDK"
     gene            4352..5497
                     /gene="cap8P"
                     /locus_tag="NBH32_000270"
     CDS             4352..5497
                     /gene="cap8P"
                     /locus_tag="NBH32_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8P"
                     /protein_id="HCD1513909.1"
                     /translation="MKKIMVIFGTRPEAIKMAPLVKEIDHNGNFEANIVITAQHRDML
                     DSVLSIFDIQADHDLNIMQDQQTLADLTANTLAKLDSIINEEQPDMILVHGDTTTTFV
                     GSLAAFYHQIPVGHVEAGLRTHQKYSPFPEELNRVMVSNIAELNFAPTVIAAKNLLFE
                     NKDKESIFITGNTVIDALSTTVQNDFVSTIINKHKGKKVILLTAHRRENIGEPMHQIF
                     KAVRDLADEYKDVVFIYPMHRNPKVRAIAEKYLSGRNRIELIEPLDAIEFHNFTNQSY
                     LVLTDSGGIQEEAPTFGKPVLVLRNHTERPEGVEAGTSRVIGTDYDNIVRNVKQLIED
                     DEAYQRMSQANNPYGDGQASQRICEAIEYYFGLRSDKPDEFIPLRHK"
     gene            complement(5562..5888)
                     /gene="isdI"
                     /locus_tag="NBH32_000271"
     CDS             complement(5562..5888)
                     /gene="isdI"
                     /locus_tag="NBH32_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdI"
                     /protein_id="HCD1513910.1"
                     /translation="MFMAENRLQLQKGSAEETIERFYNRQGIETIEGFQQMFVTKTLN
                     TEDTDEVKILTIWESEDSFNNWLNSDVFKEAHKNVRLKSDDDGQQSPILSNKVFKYDI
                     GYHYQK"
     gene            complement(5895..6278)
                     /locus_tag="NBH32_000272"
     CDS             complement(5895..6278)
                     /locus_tag="NBH32_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaN family protein"
                     /protein_id="HCD1513911.1"
                     /translation="MRLILIVIGLILTALGIAGAVLPLLPTTPFLLVAVFCFARSSDR
                     FYNWLINQKIYKEYVENFYLHRGYTLQQKIKILISLYIVIGFSIYMVDVLAVRVGLII
                     MVIIQTAVLFTFVKTLPKSNHKIEE"
     gene            6704..8191
                     /locus_tag="NBH32_000273"
     CDS             6704..8191
                     /locus_tag="NBH32_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCD1513912.1"
                     /translation="MAVNVRDYIAENYGLFINGEFVKGSSDETIEVTNPATGETLSHI
                     TRAKDKDVDYAVEVAQEAFESWSLTSKSERAQMLRDICDKLMAQKDKIAMIETLNNGK
                     PIRETTAIDIPFAARHFHYFASVIETEEGTVNDIDKDTMSIVRHEPIGVVGAVVAWNF
                     PMLLAAWKIAPAIAAGNTIVIQPSSSTPLSLLEVAKIFQEVLPKGVVNILTGKGSESG
                     NAIFNHDGVDKLSFTGSTDVGYQVAEAAAKHLVPATLELGGKSANIILDDANLDLAVE
                     GIQLGILFNQGEVCSAGSRLLVHEKIYDQLVPRLQEAFSNIKVGDPQDEATQMGSQTG
                     KDQLDKIQSYIDAAKESDAQILAGGHRLTENGLDKGFFFEPTLIAVPDNHHKLAQEEI
                     FGPVLTVIKVKDDQEAIDIANDSEYGLAGGVFSQNITRALNIAKAVRTGRIWINTYNQ
                     VPEGAPFGGYKKSGIGRETYKGALSNYQQVKNIYIDTSNALKGLY"
     gene            8838..9797
                     /locus_tag="NBH32_000274"
     CDS             8838..9797
                     /locus_tag="NBH32_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1513913.1"
                     /translation="MNGKKANTINRYKYFHHVDHQKIQQSSKKTLWASLIITLLFTVI
                     EFVGGLVSNSLALLSDSFHMLSDVLALGLSMLAIYFASKKPTARYTFGYLRFEILAAF
                     LNGLALIVISIWILYEAIVRIIYPQPIESGIMFMIASIGLLVNIILTVILVRSLKQED
                     NINIQSALWHFMGDLLNSIGVIVAVVLIYFTGWRIIDPIISIVISLIILRGGYKITRN
                     AWLILMESVPQHLDTDQIMADIKNIDGILDVHEFHLWSITTEHYSLSAHVVLDKKYEG
                     DDYQAIDQVSSLLKEKYGIAHSTLQIENLQLNPLDEPYFDKLT"
     gene            complement(9859..10062)
                     /locus_tag="NBH32_000275"
     CDS             complement(9859..10062)
                     /locus_tag="NBH32_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513914.1"
                     /translation="MDKESVVASLARNKKIAVETMTGQRYIIERILHTNDEKHIHILK
                     PKDVVLDVDTIKDIDENHLDDAT"
     gene            10241..10753
                     /locus_tag="NBH32_000276"
     CDS             10241..10753
                     /locus_tag="NBH32_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HCD1513915.1"
                     /translation="MTLFLLEANNLDFASTKEELEAKAASLSTKTIPTLIEVQATENL
                     THGYFIVEANDEAEAKQFLTEADISIQLVKEVRLVGKDLDEVKNGDAHVDYLVTWNIP
                     EGITMDQYLARKKKNSVHYEEVPEVEFKRTYVCEDMSKCICLYNAPDEEAVRRARKAV
                     DTPIDGIEKL"
     gene            11095..11835
                     /locus_tag="NBH32_000277"
     CDS             11095..11835
                     /locus_tag="NBH32_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1513916.1"
                     /translation="MIKIQQLQHHFGSHKVIHNFNLDISKGEIVTFIGKSGCGKSTLL
                     NIIGGFIHPSSGRVIIDNEIKQQPSPDCLMLFQHHNLLPWKTINDNIRIGFQQKISDE
                     EINAQLKLVDLEGRGKHFPEQLSGGMKQRVALCRAHVHKPNVILMDEPLGALDAFTRY
                     KLQDQLVQLKHKTQSTIILVTHDIDEAIYLSDRIVLLGEGCNIISQYEITASHPRSRN
                     DSHLLKIRNEIMETFALNHHQVEPEYYL"
     gene            11849..12823
                     /locus_tag="NBH32_000278"
     CDS             11849..12823
                     /locus_tag="NBH32_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1513917.1"
                     /translation="MKRLSIIVIIGIFIITGCDWQRTSKEPSKNAQNQQVIKIGYLPI
                     THSANLMMTKKLLSQYNHPKYKLELVKFNNWPDLMDALNSGRIDGASTLIELAMKSKQ
                     KGSNIKAVALGHHEGNVIMGQKGMHLNEFNNNGDDYHFGIPHRYSTHYLLLEELRKQL
                     KIKPGHFSYHEMSPAEMPAALSEHRITGYSVAEPFGALGEKLGKGKTLKHGDDVIPDA
                     YCCVLVLRGELLDQHKDVAQAFVQDYKKSGFKMNDRKQSVDIMTHHFKQSRDVLTQSA
                     AWTSYGDLTIKPSGYQEITTLVKQHHLFNPPAYDDFVEPSLYKEASRS"
     gene            12820..13581
                     /locus_tag="NBH32_000279"
     CDS             12820..13581
                     /locus_tag="NBH32_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1513918.1"
                     /translation="MTRPTNNKFILPIITFIIFLGIWEMVIIIGHYQPVLLPGPVLVG
                     KSIWTFIVTGEIFQHLAISLWRFVAGFVVALLVAIPLGFLLGRNRWLYNAIEPLFQLI
                     RPISPIAWAPFVVLWFGIGSLPAIAIIFIAAFFPIVFNTIKGVRDIEPQYLKIAANLN
                     LTGWSLYRNILFPGAFKQIMAGIHMAVGTSWIFLVSGEMIGAQSGLGFLIVDARNMLN
                     LEDVLAAIFFIGLFGFIIDRFISYIEQFILRRFGE"
     gene            13594..14625
                     /locus_tag="NBH32_000280"
                     /pseudo
     CDS             13594..14625
                     /locus_tag="NBH32_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498739.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
     gene            14842..15201
                     /locus_tag="NBH32_000281"
     CDS             14842..15201
                     /locus_tag="NBH32_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCD1513919.1"
                     /translation="MNTIDTHTKEQQFSNLVRSYRKEYVGKGPNSIRVSFKDNWAIAH
                     MTGVLSKVESFYLNDKRNESMLHYTRTEKIKQMYKEIDVNEMESLVGAKFVKLFTDID
                     LNDDEVISIFVFDKSIE"
     gene            15374..16399
                     /locus_tag="NBH32_000282"
     CDS             15374..16399
                     /locus_tag="NBH32_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent formate dehydrogenase"
                     /protein_id="HCD1513920.1"
                     /translation="MKIVALFPEAVEGQENQLLNTKKALGLKTFLEERGHEFIILADN
                     GEDLDKHLPDMDVIISAPFYPAYMTRERIEKAPNLKLAITAGVGSDHVDLAAASEHNI
                     GVVEVTGSNTVSVAEHAVMDLLILLRNYEEGHRQSVEGEWNLSQVGNHAHELQHKTIG
                     IFGFGRIGQLVAERLAPFNVTLQHYDPINQQDHKLSKFVSFDELVSTSDAITIHAPLT
                     PETDNLFDKDVLSRMKKHSYLVNTARGKIVNRDALVEALASEHLQGYAGDVWYPQPAP
                     ADHPWRTMPRNAMTVHYSGMTLEAQKRIEDGVKDILERFFNHEPFQDKDIIVASGRIA
                     SKSYTAK"
     gene            16785..18035
                     /locus_tag="NBH32_000283"
     CDS             16785..18035
                     /locus_tag="NBH32_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1513921.1"
                     /translation="MKRLSTTLKVRLISNFLQLIITTAFIPFIALYLTDMLSQSIVGI
                     YLVGLVVLKFPLSIISGYLIEIFPKKLLVLIYQATMVIMLVFMGIFGSHQLWQIIGFC
                     VAYAIFTIVWGLQFPVMDTLIMDAITEDVEHYIYKISYWMTNLSVAIGALLGGLMYGY
                     SMLLLFLIAACIFLIVLFILYIWLPQDRNQVKQSDDKRHASRYQKLQIMNIFRSYKLV
                     LKDRNYMLLISGFSIIMMGEFSISSYIAIRLKNQFETISIGSYDITGAKMLAILLMIN
                     TVVVILLTYSISKVVLKIDFKKALITGLLIYIVGYSGLTYLNQFGLLVVFMIIATVGE
                     IIYSPIVSEQRFKIIPKAKRGTYSAVNALGIHFSETLARLGIVLGAFLTSLQMGLYMF
                     IVLTIGASMLVAGVFGGQKQVNTN"
     gene            18482..25657
                     /locus_tag="NBH32_000284"
     CDS             18482..25657
                     /locus_tag="NBH32_000284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid adenylation domain-containing
                     protein"
                     /protein_id="HCD1513922.1"
                     /translation="MIMGNLRFQQEYFRIYKNNTESTTHRNAYWVKLAKNVEATKMMY
                     ALSTIVQQHASIRHFFDVTTDDNLTMILHEFLPFIEIKQVPTSSANYDLEAFFKQELS
                     TYHFNDSPLFKFKLFQFADAAYILLDFHVSIFDDSQIDIFLDDLCNAYRGNTVINNTR
                     QHAHLNRNDDKHNQDASHIALDSNYFRLENNSDIHIDSYLPIKHPFEQALYQTYLIDD
                     MTSIDMASLAVSVYLANHIMSQQHDVTLGIHVPSHLPNDLHGNIVPLTLTIDAKDVCQ
                     RFTTDFNKCVLQNMSQLQCANSSLSLETIFHCYHHMMSCCNDVIEDVHQIHDAHTSLA
                     DIEIFPHQHGFKIIYNSAAYDLLSIETLSDLVRNIYLQITEENGNKRTTVDELNLMTE
                     RDIQLYDDINVSLPEIDDAQTVVTLFEQQVEATPNHVAVQFDGVFITYQTLNARANDL
                     AHRLRNQYGVEPNDRVAVIAEKSIEMIIAMIGVLKAGGAYVPIDPNYPSDRQEYILKD
                     ATPKVVITYQALYENGKQNINHIDLNKIAWKNIDNLSKCNTLEDHAYVIYTSGTTGNP
                     KGTLIPHRGIVRLVHQNHYVPLNEETTILLSGTIAFDAATFEIYGALLNGGKLIVAKK
                     EQLLNPIALEQLINENDVNTMWLTSSLFNQIASERIEVLVPLKYLLIGGEVLNAKWVD
                     LLNQKPKHPQIINGYGPTENTTFTTTYNIPNKVPNRIPIGKPILGTHVYIMQGECRCG
                     VGIPGELCTSGFGLAAGYLNQPELTADKFIKDSNINQLMYRSGDIVRLLPDGNIDYLY
                     RKDKQVKIRGFRIELSEVEHALERIQGINKAVVIVQNHDQDQYIVAYYEAMHTLSHNK
                     IKSQLRMTLPEYMIPVNFMHIEQIPITINGKLDKKALPIMDYVDTDAYVAPSTDTEHL
                     LCQIFADILHVNQVGIHDNFFELGGHSLKATLVVNRIEASTGKRLQIGDLLQKPTVFE
                     LAQAIAKVQEQNYEVIPEAIVKDDYVLSSAQKRMYLLWKSNHKDTVYNVPFLWRLSSG
                     LNVAQLQQAVQHLIARHEILRTQYIVVDDEVRQRIVADVVADFEEVNTHFTDEQEIMR
                     QFVAPFNLEKPSQIRVRYIRSPLHAYLFIDTHHIINDGMSNIQLMNDLNALYQHKLLL
                     PLKLQYKDYSEWMSHRDMTKHRQYWLSQFKDEVPILSLPTDYVRPNIKTTNGAMMSFT
                     MNQQMRQLLQKYVEKHQITDFMFFMSVVMTLLSRYARKDDVVVGSVMSARMHKGTEQM
                     LGMFANTLVYRGQPSPDKMWTQFLQEVKEMSLEAYEHQEYPFECLVNDLDQSHDASRN
                     PLFDVMLVLQNNETNHAHFGHSKLTHIQPKSVTAKFDLSFIIEEDRDDYIINIEYNTD
                     LYHSETVRHMGNQCMIMIDYILKHQDTLQICDIPNGTEELLNWVNTHVNDRMLNVPGN
                     KSIISYFNEAVSRQGNHVALVMNDLTMTYETLHNYVDAIAHMLLSNGVGNGQRVALFT
                     ERSFEMIAAMLATVKVGASYIPIDIDFPNKRQGAILEDAKVTAVMSYGVEIETTLPVI
                     QLENAKGFVESKENEQYDDLHGNQLENTAMLDNEMYAIYTSGTTGMPKGVAIRQRNLL
                     NLVHAWSTELQLGNNEVFLQHANIVFDASVMEIYCCLLNGHTLVIPDREERVNPAQLQ
                     QLINKHRVTVASIPLQMCSVMEDFYIEKLITGGATSTASFVKYIEKHCGTYFNAYGPS
                     ESTVITSYWSHHCGDLIPETIPIGKPLSNIQVYIMSDGLLCGIGMPGELCIAGDSLAI
                     GYINRPELMADKWQNNPFGKGKLYHSGDLARYTSDGQIEFLGRIDKQVKVNGYRIELD
                     EIENAILAIRGISDCVVTVSHFDTHDILNAYYVGEQQVEQDLKQYLNDHLPKYMIPKT
                     ITHIDCMPLTTNDKVDTTRLPNPSPIQQSNKVYSEPSNEIEQTFVDVFGEVLKQNDVG
                     VDDDFFELGGNSLEAMLVVSHLKRFGHHISMQTLYQYKTVRQIVNYMYQNQQSLVALP
                     DNLSELQKIVMSRYNLGILEDSLSHRPLGNTLLTGVTGFLGAYLIEALQGYSHRIYCF
                     IRADNEEIAWYKLMTNLNDYFSEETVEMMLSNIEVIVGDFECMDDVVLPENMDTIIHA
                     GARTDHFGDDDEFEKVNVQGTVDAIRLAQQHHARLIYVSTISVGTYFDIDTEDVTFSE
                     ADVYKGQLLTSPYTRSKFYSELKVLEAVNNGLDGRIVRVGNLTSPYNGRWHMRNIKTN
                     RFSMVMNDLLQLDCIGVSLAEMPVDFSFVDTTARQIVALAQVNTPQIIYHVLSPNKMP
                     VKSLLECVKRKEIELVSDESFNEILQKQDMYETIGLTSVDREQQLAMIDTTLTLKIMN
                     HISEKWPTITNNWLHHWAQYIKTIFNK"
     gene            25670..26314
                     /locus_tag="NBH32_000285"
     CDS             25670..26314
                     /locus_tag="NBH32_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCD1513923.1"
                     /translation="MTVFVMQLQSNLKSIEELISQSRWSYKKPRTVNYRYNQDKLMHR
                     LGDILVQYGIQHDTGLLPHEWHYHISPRGKADIVQHNRDGQPIYVSLSYSYPYIVCVV
                     DKEPVGIDIEKISQRLDWRTLVTCFSTNEAHQICSLNDFYQIWTQKESFTKLIGEGLI
                     KGLDIYDMTQSHFYQSREVKFKQFIFDQFMVQVCFLGQAPWGYKKVSVFQLLSS"
     gene            complement(26642..27121)
                     /locus_tag="NBH32_000286"
     CDS             complement(26642..27121)
                     /locus_tag="NBH32_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YagU family protein"
                     /protein_id="HCD1513924.1"
                     /translation="MGIFIYAGIIGGLLSGIVKLGWEVMFPPRTPERNATNPPQELLQ
                     QLGFSSEFTHQTYTFSNMELPWVSFIVHFSFSIVIAIIYCILVKKYAYLAMGQGAVFG
                     IAIWVLFHLIIMPIMHTVPAVWDQPFQEHLSEFFGHIVWMMTIELVRQHFVYRYKLN"
     gene            complement(27389..28153)
                     /gene="argB"
                     /locus_tag="NBH32_000287"
     CDS             complement(27389..28153)
                     /gene="argB"
                     /locus_tag="NBH32_000287"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCD1513925.1"
                     /translation="MKFIVIKIGGSTLSDMHPSIINNIKHLRSNNIYPIIVHGGGPFI
                     NDALSNQQIEPHFVNGLRVTDKATMTITKRTLIADVNTALVAQFNQHQCSAIGLCGVD
                     AQLFEITSFDQQYGYVGVPTALNKDALQYICDKFVPIINSIGFNNHDGEFYNINADTL
                     AYFIASSLKAPIYVLSNIAGVLINDVVIPHLPLGDINQYIEHGDIYGGMIPKVLDAKN
                     AIENGCPKVIIASGNKPNIIESIYNNDFVGTTILNS"
     gene            complement(28169..29410)
                     /gene="argJ"
                     /locus_tag="NBH32_000288"
     CDS             complement(28169..29410)
                     /gene="argJ"
                     /locus_tag="NBH32_000288"
                     /EC_number="2.3.1.1"
                     /EC_number="2.3.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate
                     N-acetyltransferase/amino-acid acetyltransferase ArgJ"
                     /protein_id="HCD1513926.1"
                     /translation="MKHQETTSQQYNFSIIKHGDISTPQGFTAGGMHIGLRANKKDFG
                     WIYSSSLASAAAVYTLNQFKAAPLIVTEDTLQKSKGKLQALVVNSANANSCTGQQGID
                     DARQTQTWVAQQLQIPSEHVAVASTGVIGEYLPMDKIKNGTDHIKDTKYTTPGAFNEA
                     ILTTDTCTKHIAVSLKIDGKTVTIGGSAKGSGMIHPNMATMLAFITTDAAIESNTLHQ
                     LLKSSTDHTFNMITVDGDTSTNDMVLVMANHQVEHQILNQDHPQWETFVDAFNFVCTF
                     LAKAIARDGEGATKLISVNVSGAKSISDARKIGKTIVSSNLVKSAIFGEDANFGRIIT
                     AIGYSGCEIDPNCTYVQLNQIPVVDKGMAVLFDEQAMSNTLTHEHVTIDVQLGLGNAT
                     ATAYGCDLSYDYVRINASYRT"
     gene            complement(29422..30453)
                     /locus_tag="NBH32_000289"
     CDS             complement(29422..30453)
                     /locus_tag="NBH32_000289"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCD1513927.1"
                     /translation="MIKVGIVGGSGYGAIELIRLLQTHPHITIAHIYSHSKVDEPLKL
                     TFPHLQHIMQHFEALTVDNNDCDVIFFATPAPVSKTCIPPLVEKGIHVIDLSGAFRIK
                     NREIYEAYYKETAAAQDDLNHAIYSISEWQSFDNNGTKLISNPGCFPTATLLALHPLI
                     SEKIVDLSSIIIDAKTGVSGAGRSLSQRVHFSEMNENLSAYAIGNHKHKPEIEQYLSI
                     VAGQDVSVIFTPHLVPMTRGILSTIYVKLSSEYTTESLHKLMTSYYANQPFVRIRDIG
                     TFPTTKEVLGSNYCDIGIYVDETTQTAILVSVIDNLVKGASGQAIQNLNILYNFEVTT
                     GLKQSPVYP"
     gene            complement(30492..31676)
                     /gene="rocD"
                     /locus_tag="NBH32_000290"
     CDS             complement(30492..31676)
                     /gene="rocD"
                     /locus_tag="NBH32_000290"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD1513928.1"
                     /translation="MNSIIELTDYYSSNNYAPLKLVISKGKGVKVWDTDGKQYIDCIS
                     GFSVANQGHCHPTIVKAMTEQASKLSIISRVLYSDNLGKWEEKICHLAKKDKVLPLNS
                     GTEAVEAAIKIARKWGSEVKGITDGQVEIIAMNNNFHGRTLGSLSLSNHDAYKAGFHP
                     LLQGTTTVDFGDIEQLTQAISPNTAAIILEPIQGEGGVNIPPKGYIQAVRQLCDKHQI
                     LLIADEIQVGLGRTGKWFAMEWEQVVPDIYILGKALGGGLYPVSAVLANNDVMRVLTP
                     GTHGSTFGGNPLAIAISTAALDVLKDEQLVERSERLGSFLLKALLQLKHPSIKEIRGR
                     GLFIGIELNTDAAPFVDQLIQRGILCKDTHRTIIRLSPPLVIDREEINQIVAAFQDVF
                     KN"
     gene            complement(31929..33284)
                     /gene="brnQ"
                     /locus_tag="NBH32_000291"
     CDS             complement(31929..33284)
                     /gene="brnQ"
                     /locus_tag="NBH32_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1513929.1"
                     /translation="MKKKLTFKENMFIGSMLFGLFFGAGNLIFPIHLGQAAGSNVFIA
                     NLGFLITAIGLPFLGIIAIGISKTSGLFEIASRVNKTYAYIFTIALYLVIGPFFALPR
                     LATTSFEIAFSPFLSPKQITLYLFIFSFVFFVIAWFFARKPSRILEYIGKFLNPVFLV
                     LLAIILLFAFIHPLGGISDAPISKQYQSHALFNGFLDGYNTLDALASLAFGIIIVATI
                     KKLGIENPTDIAKETIKSGTISIIMMGIIYTLLAIMGTLSIGHFKLSENGGIALAQIT
                     QYYLGNYGIVLLSLIVMVACLKTAIGLITAFSETFEHLFPKMNYLAIATVVSFISFLF
                     ANVGLTKIIMYSVPVLMFLYPLAIALIVLTLFSSKFHHSKIIYQCTIFFTMIAALVDG
                     LKASPEFISSTSFSQTLINFSQKYLPLSDIGMGWVVLSLIGFIIGFIIFKIKHRKIPQ
                     A"
     gene            complement(33560..34117)
                     /locus_tag="NBH32_000292"
     CDS             complement(33560..34117)
                     /locus_tag="NBH32_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCD1513930.1"
                     /translation="MINFNKTALVLIDLQEGILKMDYAPHTAENVVQNANKLIDVFRK
                     NNGFIAFVRVNFYDGKDVLQPNAMISLPPKEGDDYSRFHHLLDKRDDDFVIDKRHFSA
                     FVGTDLDLQLRRRGIDTIVLGGVATHIGVDTTARDAYQLNYNQFFVTDMMSAQNETLH
                     QFPIDNVFPLMGQTITTNDFLNILN"
     gene            complement(34184..35824)
                     /locus_tag="NBH32_000293"
     CDS             complement(34184..35824)
                     /locus_tag="NBH32_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-keto acid decarboxylase family protein"
                     /protein_id="HCD1513931.1"
                     /translation="MKQRIGAYLIDAIHRAGVDKIFGVPGDFNLAFLDDIISNPNVDW
                     VGNTNELNASYAADGYARLNGLAALVTTFGVGELSAVNGIAGSYAERIPVIAITGAPT
                     RAVEQAGKYVHHSLGEGTFDDYRKMFAHITVAQGYITPENATTEIPRLINTAIAERRP
                     VHLHLPIDVAISEIEIPTPFEVTATKDTDASTYIELLATKLHQAKQPIIITGHEINSF
                     HLHQELEDFVNQTQIPVAQLSLGKGAFNEENPYYMGIYDGKIAEDKIRDYVDNSDLIL
                     NIGAKLTDSATAGFSYQFNIDDVVMLNHHNIKIDDFTNDEISLPSLLKQLSNISYTNN
                     ASFPAYHRPTSPDYTVGTEPLTQQTYFKMMQNFLKPNDVIIADQGTSFFGAYDLALYK
                     NNTFIGQPLWGSIGYTLPATLGSQLADKDRRNLLLIGDGSLQLTVQAISTMIRQHIKP
                     VLFVINNDGYTVERLIHGMYEPYNEIHMWDYKALPAVFGGKNVEIHDVESSKDLQDTF
                     NAINGHPDVMHFVEVKMSVEDAPKKLIDIAKAFSQQNK"
     gene            36017..36118
                     /locus_tag="NBH32_000294"
     CDS             36017..36118
                     /locus_tag="NBH32_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513932.1"
                     /translation="MIPQNTIAKKKQARHLMKVYAPVYSKHYLASNN"
     gene            complement(36097..38142)
                     /locus_tag="NBH32_000295"
     CDS             complement(36097..38142)
                     /locus_tag="NBH32_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD1513933.1"
                     /translation="MRKKLFGQLQRIGKALMLPVAILPAAGLLLAIGTAMQGESLQHY
                     LPFIQNGGVQTVAKLMTGAGGIIFDNLPMIFALGVAIGLAGGDGVAAIAAFVGYIIMN
                     KTMGDFLQVTPKNIGDPASGYASILGIPTLQTGVFGGIIIGALAAWCYNKFYNINLPS
                     YLGFFAGKRFVPIMMATTSFILAFPMALIWPTIQTGLNAFSTGLLDSNTGVAVFLFGF
                     IKRLLIPFGLHHIFHAPFWFEFGSWKNAAGEIIHGDQRIFIEQIREGAHLTAGKFMQG
                     EFPVMMFGLPAAALAIYHTAKPENKKVVAGLMGSAALTSFLTGITEPLEFSFLFVAPL
                     LFFIHAVLDGLSFLTLYLLDLHLGYTFSGGFIDYFLLGILPNKTQWWLVIPVGLVYAV
                     IYYFVFRFLIVKLKYKTPGREDKQSQAATASATELPYAVLEAMGGKANIKHLDACITR
                     LRVEVNDKSKVDVPGLKNLGASGVLEVGNNMQAIFGPKSDQIKHEMQQIMNGQVVENP
                     TTMEDDKDETVVVAEDKSATSELSHIVHAPLTGEVTPLSEVPDQVFSEKMMGDGIAIK
                     PSQGEVRAPFNGKVQMIFPTKHAIGLVSDSGLELLIHIGLDTVKLNGEGFTLHVEEGQ
                     EVKQGDLLINFDLDYIRNHAKSDITPIIVTQGNITNLDFKQGEHGNISFGNQLFEAK"
     gene            38727..39782
                     /locus_tag="NBH32_000296"
     CDS             38727..39782
                     /locus_tag="NBH32_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000145567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HCD1513934.1"
                     /translation="MTGFSVYLGQPLDEAYIKRMIKQGYQMIFTSVQIPEEDDETKYH
                     YFTKLLNLLKHEQVTYLIDANPSILTPSFYEHLRQYDAQFMIRIDHSTSIEAIEAIMA
                     QGLKCCLNASIISRELLTSLHQQLNDFTLLSFCHNYYPRPDTGLSVDLVNKKNELIYQ
                     FNPKAQIYGFIVGSGLRGPLHKGLPTIEATRHSHPVVAAKLLQETGVSEVLVGDSLIE
                     MRQAKQLIDFCKHRHFTLCIEEVFDTTVTYLFDMCHKVRPDNPENVIRSETSRQICPH
                     SIQPQFTTQRRIGSVTVDNLNNGRYQGEMQIVRQTLSAHDNVNVVAQIIKEDLPLLSC
                     IEPNDTFDFQKTRECKK"
     gene            39782..40678
                     /gene="murQ"
                     /locus_tag="NBH32_000297"
     CDS             39782..40678
                     /gene="murQ"
                     /locus_tag="NBH32_000297"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCD1513935.1"
                     /translation="MENSTTEARNEATMHLDEMTVEEALITMNKEDQQVPLAVRKAIP
                     QLTKVIKKTIAQYKKGGRLIYIGAGTSGRLGVLDAAECVPTFNTDPHEIIGIIAGGQH
                     AMTMAVEGAEDHKKLAEEDLKNIDLTSKDVVIGIAASGKTPYVIGGLTFANTIGATTV
                     SISCNEHAVISEIAQYPVEVKVGPEVLTGSTRLKSGTAQKLILNMISTITMVGVGKVY
                     DNLMIDVKATNQKLIDRSVRIIQEICAITYDEAMALYQVSEHDVKVATVMGMCGISKE
                     EATRRLLNNGDIVKRAIRDRQP"
     gene            40690..42144
                     /locus_tag="NBH32_000298"
     CDS             40690..42144
                     /locus_tag="NBH32_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1513936.1"
                     /translation="MTKEQQLAERIIAAVGGMDNIDSVMNCMTRVRIKVLDENKVDDQ
                     ELRHIDGVMGVIHDERIQVVVGPGTVNKVANHMAELSGVKLGDPIPHHHNDSEKMDYK
                     SYAADKAKANKEAHKAKQKNGKSNKVLKSIANIFIPLIPAFIGAGLIGGIAAVLSNLM
                     VAGYISGAWITQLITVFNVIKDGMLAYLAIFTGINAAKEFGATPGLGGVIGGTTLLTG
                     IAGKNILMNVFTGEPLQPGQGGIIGVIFAVWILSIVEKRLHKIVPNAIDIIVTPTIAL
                     LIVGLLTIFIFMPLAGFVSDSLVSVVNGIISIGGVFSGFIIGASFLPLVMLGLHHIFT
                     PIHIEMINQSGATYLLPIAAMAGAGQVGAALALWVRCKRNTTLRNTLKGALPVGFLGI
                     GEPLIYGVTLPLGRPFLTACIGGGIGGAVIGGIGHIGAKAIGPSGVSLLPLISDNMYL
                     GYIAGLLAAYAGGFVCTYLFGTTKAMRQTDLLGD"
     gene            42147..43022
                     /locus_tag="NBH32_000299"
     CDS             42147..43022
                     /locus_tag="NBH32_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1513937.1"
                     /translation="MTNILYRIDKQLSDFTKTEKIIADYILKNPHKIIDMTVNDLADV
                     TNVSTASIVRFSRKMTHQGFQELKIAISRYLPEDIATNPHLELIENEPVETLKNKMIA
                     RATNTMRFVATNIMDAQIDAICDVLKNARTIFLFGFGASSLTIGDLFQKLSRIGLNVR
                     LLHETHLLVSTFATHDDRDCMIFVTNQGSHSELQSIAQVATHYSIPIITISSTANNPV
                     AQIADYALIYGRTDENEMRMAATTSLFAQLFTVDILYYRFVALNYHAILDCITQSKMA
                     LDNYRKHLATIDFKH"
BASE COUNT        14583 a         6714 c         8267 g        13960 t
ORIGIN      
        1 taacattgat tcaagtttat attcgaagtt tattatttta ggtaatatag gtgttttatt
       61 caatgcagtg agtattatga tccaaacttt aaatacaaaa cacgcatcaa taacattaca
      121 agcgaattat atgacgtttc acacgattac atttatattc ataactattt taatgacaat
      181 tgcgtttggt ctaaatggat tcttttggac aacgctgttc agcaacatta ttaagtatgt
      241 gattttaaat attataggtt taaagtctaa attcattaat aaaaaggacg tcgattagat
      301 gagtgaaaaa aagattttga ttttatgtca gtatttttat ccggaatatg tatcttctgc
      361 gacgttacca actcaattgg cggaagattt aattgcgaat cacattaatg tcgatgtcat
      421 gtgtggatgg ccatatgaat atagtaatca taaacaggtt tctaaaaccg agatgcatcg
      481 tggtattcgc attcgacgtc tcaagtattc gaggtttaat aacaaaagta aggttggaag
      541 gatcatcaat ttctttagtt tattttcaaa attcgtgatt aatataccta aaatgttgaa
      601 atatgatcag attcttgttt actctaatcc accaatcttg ccattaatac cagacgtttt
      661 acacagactg cttaagaaaa aatattcttt tgtggtgtat gatatagcac ctgataatgc
      721 gattaagaca ggtgcaactc gtccaggtag catgattgat aagctgatgc gttacattaa
      781 tagacatgtc tacaagaatg ctgaaaatgt cattgtcctt ggtacggaaa tgaaaaacta
      841 cttactaaat catcaaattt ctaaaaatgc tgacaatatc catgtgattc ctaactggta
      901 tgacatgcgt caattacaag acaatcgtat ctataatgat acatttaaag cttaccgtga
      961 gcaatacgac aaaattttat tgtatagcgg taatatgggg cagttacagg atatggagac
     1021 acttatctca tttttaaaat taaataagga tcagcctcaa acgttaacaa tactttgtgg
     1081 tcatggtaag aaatttgcag atgtcaaaac ggcaatagaa gaccatcgta ttgaaaatgt
     1141 taaaatgttt gagtttttaa caggtacaga ctatgctgac gtattaaaaa ttgcggatgt
     1201 atgtattgca tcgctgatta aagaaggcgt cggtttaggc gtgccgagca agaattatgg
     1261 ctaccttgca gctaagaaac cgttggtact catcatggat aagcaatctg atatcgttca
     1321 acatgttgaa caatatgatg cgggtatcca aattgataat ggcgatgcac atgccattta
     1381 taacttcatc aacactcact cgagtaagga attgcacgag atgggtgagc gcgcacatca
     1441 actgtttaaa gataaatata cgagagaaat taatactatg aagtattaca atctgttgaa
     1501 gtgaggagat aattatgaag cgattattcg atgtagtgag ttcaatatat ggtttagtag
     1561 ttttaagtcc gattctgtta attacagcat tactaattaa aatggaatca cctggaccag
     1621 ccattttcaa acaaaaaaga ccgacgatta ataatgaatt gtttaatatt tataagttta
     1681 gatcaatgaa aatagacaca cctaatgttg caactgattt aatggattca acatcgtata
     1741 taacaaagac agggaaggtc attcgtaaga cctctattga tgaattgcca caattattga
     1801 atgttttaaa aggagaaatg tcaattgtag gtcctagacc agcgctttat aatcaatacg
     1861 aattaatcga aaaacgtaca aaagcgaacg tgcatacgat tagaccaggt gtgacaggac
     1921 tagctcaagt gatggggaga gatgatatta ctgatgatca aaaagtagcg tatgatcatt
     1981 attacttaac acatcaatct atgatgcttg atatgtatat catatataaa acaattaaaa
     2041 atatcgttac ttcagaaggt gtgcatcact aatgagaaaa aatattttaa ttacaggcgt
     2101 acatggatat atcggtaatg ctttaaaaga taagcttatt gaacaaggac atcaagtaga
     2161 tcaaattaat gttaggaatc aattatggaa gtcgacctcg ttcaaagatt atgatgtttt
     2221 aattcataca gcagctttgg ttcacaacaa ttcacctcaa gcaaggctat ctgattatat
     2281 gcaagtgaat atgttgctta cgaaacaatt ggcacaaaag gctaaagctg aagacgttaa
     2341 acaatttatt tttatgagta ctatggcagt ttatggaaaa gaaggtcagg ttggtaaatc
     2401 agatcaaatt gatacacaaa caccaatgaa ccctacgacc aattatggta tttccaaaaa
     2461 gttcgctgaa caagcattac aagagttgat tagtgattcg tttaaagtag caattgtgag
     2521 accaccaatg atttatggtg cacattgccc aggaaatttc caacggttaa tgcaattgtc
     2581 aaagcgattg ccaatcattc caaatattaa caatcagcgc agtgcattat atattaaaca
     2641 tctgacagca tttattgatc aattaatatc attagaagtg acaggcgtgt atcatcctca
     2701 agatagtttt tactttgata catcgtcagt aatgtatgaa atacgtcgcc aatcacatca
     2761 taaaacggta ttgatcaaca tgccttcaat gctaaataag tattttaata agttgttggt
     2821 ctttagaaaa ttattcggca atttaatata cagcaatacg ttatatgaaa ataataatgc
     2881 acttgaagtt attcctggaa aaatgtcact tgttattgcg gacatcatgg atgaaacgac
     2941 aaccaaagat aaggcataag tcatctatta aataaaatca acatacaaat cgttttattt
     3001 ggaggttata gtatgaagtt aacagtagtt ggcttaggtt atattggttt accaacatca
     3061 attatgtttg caaagcatgg cgtcgatgtg cttggtgttg atattaatca gcaaacgatt
     3121 gataagttac aaagtggtca aattagtatt gaagaacctg gattacaaga ggtttatgaa
     3181 gaggtactgt catcgggaaa attgaaggta tctacaacgc cagaagcatc tgatgttttt
     3241 atcattgccg ttccgacgcc gaataatgat gatcagtacc ggtcatgtga catttcgcta
     3301 gttatgcatg cattagatag tattttacca tttttagaaa aaggaaatac cattattgta
     3361 gagtcgacaa ttgcgcctaa aacgatggat gattttgtaa aaccggtcat tgaaaattta
     3421 gggtttacaa taggtgaaga tatttattta gtacattgtc cagaacgtgt actgccagga
     3481 aaaattttag aagaattagt tcataacaat cgcatcattg gcggtgtgac taaagcttgt
     3541 attgaagcgg gtaaacgtgt ctatcgcaca ttcgttcagg gagaaatgat tgaaacagat
     3601 gcacgtactg ctgaaatgag taagctaatg gaaaacacat atagagacgt gaacatttct
     3661 ttagctaatg aattaacaaa aatttgcaat aacttaaata ttaatgtatt agatgtgatt
     3721 gaaatggcaa acaaacatcc gcgtgttaat atccatcaac ctggtccagg tgtaggcggt
     3781 cattgtttag ctgttgatcc gtactttatt attgctaaag accctgaaaa tgcaaagtta
     3841 attcaaactg gacgtgaaat taataattca atgccggcct atgttgttga tacaacgaaa
     3901 caaatcatta aagcgttgag cgggaataaa gtcacagtat ttggtttaac ttataaaggt
     3961 gatgttgatg atataagaga atcaccagca tttgatattt atgagctatt aaatcaagaa
     4021 ccagacatag aagtatgtgc ttatgatcca catgttgcat tagattttgt agaacatgat
     4081 atgtcacatg ctgtcaaaga cgcatcgcta gtattgattt taagtgacca ctcagaattt
     4141 aaaaatttat cggacagtca ttttgataaa atgaagcata aagtgatttt tgatacaaaa
     4201 aatgttgtga aatcatcatt tgaagatgta tcgtattata attatggcaa tatatttaat
     4261 tttatcgaca aataaaatgt gtcaaactag ggcatacatg attaaggaaa gataagctgt
     4321 tatgtgtttg aacttcagag aggataatgt tatgaaaaaa attatggtta ttttcggtac
     4381 gagacccgaa gcaataaaaa tggcaccatt agtaaaagaa attgatcata atgggaactt
     4441 tgaagcgaac attgtgatta cagcacaaca tagagatatg ttagatagtg tgttaagtat
     4501 atttgatatt caagctgatc atgatttaaa tattatgcaa gatcaacaaa cgttagcgga
     4561 ccttaccgcg aatacgcttg ctaaacttga tagcatcatt aatgaggaac agccggatat
     4621 gattttagta catggtgata ctacaacgac ttttgtagga agtttggcag cattttatca
     4681 tcaaattccg gtcggacatg tagaagctgg acttcgaaca catcagaaat actcgccatt
     4741 tcctgaagag ttaaatcgag tcatggtaag taatattgct gaattgaatt ttgcaccaac
     4801 agtaattgca gctaaaaatt tactttttga aaacaaagac aaagagagta tctttattac
     4861 tggaaataca gttattgacg cattgtcaac aacagttcaa aatgattttg tttcaacgat
     4921 tattaataaa cataaaggca agaaagttat tttactaaca gcgcatcgtc gtgaaaatat
     4981 tggggaaccg atgcatcaga tttttaaagc agtaagagat ttggcagatg aatataaaga
     5041 tgttgtcttc atttatccaa tgcatcgtaa tccaaaggta agagcgattg ccgaaaaata
     5101 tttatctggg agaaatcgga ttgaattaat tgagccatta gatgcgattg agttccataa
     5161 ttttacaaat caatcgtacc tcgtgctgac agattctggt ggtattcaag aggaggctcc
     5221 tacatttgga aaacctgtgt tggtattaag gaatcataca gagcgtcccg aaggcgttga
     5281 ggcgggaaca tcgagagtaa ttggcacaga ttatgacaat attgttcgaa atgtgaaaca
     5341 attgattgag gatgatgaag cgtatcaacg tatgagtcaa gcgaataatc catatggtga
     5401 tggacaagca tcacaacgta tttgtgaagc aatagaatat tattttggat tgcgctcaga
     5461 caagccggat gaattcatac ctttacgtca caaataataa aaacccccta atcatgaagt
     5521 tggtttagac aaccagcggt gactaggggt ttttaatata tttatttttg atagtggtag
     5581 ccaatatcat atttgaatac tttatttgat aatattggac tttgctgtcc atcgtcatca
     5641 ctttttaaac gtacattttt atgagcttct ttaaatacat cggaattcaa ccaattatta
     5701 aagctatctt cagattccca aatagttaag attttaactt cgtctgtatc ctcggtattt
     5761 aatgttttag tgacaaacat ttgttggaag ccttcaatag tttcaatacc ttgtctattg
     5821 taaaaacgtt caatcgtttc ttccgcactg cctttttgta attgtaatct attttctgcc
     5881 ataaacatgg gcaatcactc ctctatttta tgatttgatt tgggtaatgt ttttacaaat
     5941 gtaaagagta cagcggtctg tatgataacc atgatgatta atcctacacg gactgcaaga
     6001 acatccacca tataaattga aaaacctatt acaatgtata agctaattaa aattttaatt
     6061 ttctgttgta gcgtgtagcc tcgatgtaaa taaaagtttt ctacatattc tttataaatt
     6121 ttttgattaa taagccaatt gtaaaagcga tctgaacttc gagcaaaaca aaaaactgct
     6181 acgagtaaaa aaggggtcgt tggcagtaaa ggtaatacgg cacctgcaat accaagcgct
     6241 gtaagtatta agccaatgac gattaaaata agtcgcattg aaaaaactcc attctagtac
     6301 tattgcgcat gtaatattat tttagtaata taactcatgc taaatataat gtgtatgata
     6361 agtgcaatga ctcagtaaaa tgaaacgttg ttgaattatc cttgtcccat taacgcgttt
     6421 taagcgcgct ttcataacaa ctaaactatt taatgagaat tattctcaag tattatagtt
     6481 atattatgtg ttttattttt gaaaagtgca atatgttttc gaaaataaga ttatttttat
     6541 gtgcaaaaac gacgcaaaag ttttaaaaat gagacttctg tgagctgatt attttataaa
     6601 atgtaaacgc ttactatata atgtgaatca tatcgtttaa aagcattatt aaatatgatg
     6661 ctaagagatt tatattatag ccaataaaca aaggagagat aatatggcag taaacgttcg
     6721 agattatata gcagagaatt atggtttatt tatcaatggg gaatttgtta aaggtagcag
     6781 tgacgaaaca atcgaagtga ctaatccagc aactggagaa acactatcac atattacaag
     6841 agcaaaagat aaagatgtcg attatgcagt cgaagtggcg caagaggcat ttgaatcatg
     6901 gtcattaact tctaaatcag aacgtgcaca aatgttgcgt gatatttgtg ataaattaat
     6961 ggcacaaaaa gataaaattg caatgattga aacattaaat aatggtaaac cgattcgtga
     7021 gacaacagca attgatattc catttgctgc aagacatttc cattatttcg caagtgttat
     7081 tgaaacagaa gaaggtacag tgaatgatat cgataaagac acaatgagta tcgtacgaca
     7141 tgagccgatt ggcgtcgtag gtgctgttgt tgcttggaac ttcccaatgc tattagctgc
     7201 atggaagatt gcgccagcca ttgctgcagg taatacaatt gtgattcaac cttcgtcttc
     7261 aacaccatta agtttattgg aagttgctaa aattttccaa gaggtattac ctaaaggtgt
     7321 tgtcaatata ctaacgggta aaggttcaga atcaggtaat gcaattttca atcatgatgg
     7381 tgtagataaa ttatcattta cgggctcaac tgatgtaggt tatcaagttg ccgaagctgc
     7441 agcaaaacat ctagtacccg ctacattaga acttggtggt aaaagcgcca atatcatatt
     7501 agatgatgct aatttagacc ttgcagttga aggcattcag ttaggtattt tattcaacca
     7561 aggtgaagta tgtagtgcag gttctcgatt attagttcat gaaaaaattt atgatcaatt
     7621 ggtgccacgt ttacaagagg cattttcaaa tattaaagtt ggagatccac aagatgaagc
     7681 tacacaaatg ggtagtcaaa ctggtaagga tcaattagat aaaattcaat catatattga
     7741 tgcagcaaaa gaatcagatg cacaaatttt agcaggcggt catcgtttaa ctgaaaatgg
     7801 gttagataaa ggattcttct ttgaaccgac attaattgct gtgccagata atcatcacaa
     7861 attagcgcaa gaagaaatat ttggaccagt gttaacagtg attaaagtga aggacgatca
     7921 agaagcaatt gatatagcta atgattcaga gtatggttta gcaggcggcg tattttctca
     7981 aaatatcaca cgtgcattaa atattgctaa agctgttcgt acaggacgta tttggattaa
     8041 cacttacaac caagtaccag aaggcgcacc atttggtggt tataaaaaat caggtatcgg
     8101 tcgagaaact tataaaggtg cgttaagtaa ctatcaacaa gttaaaaata tttatattga
     8161 tacaagcaat gctttaaaag gtttgtacta gaataaatat cgtttctgaa gcgtgtttgc
     8221 aggcagtcta gcggtaagtc ttagcatgtt tacggagttg tttagatttt aagcgaaaca
     8281 aaatatttag gaacacgtat catgatatta ggatataatg actaaaataa tagcagtagg
     8341 atggttttta attgcaaatc atcttactgc tgtttttaat tatgctaatt tgcgatgcgg
     8401 ctattataag gacagagttg tttatgaatt atggtgattt agaaatatga agttcaatat
     8461 gcaaagtcat cgtttgtttt aatatgtgga acaatcataa aagttattgc gattttttga
     8521 acttaatgaa actaaacaat aaatttgaga tacttttttg tcatttttat gtaactcaca
     8581 caataatctc gtacattatt aaaaatttct atatgctagg gttaaagcaa agagcgagtg
     8641 tgctgcaaaa ttttttccaa ggtgatatta cataaaggta taaagggtaa agattaatga
     8701 gttgtcatga gaagaacgat ggtgtataaa ttatggttaa ttccggaagc attaatatta
     8761 atctgagaag ctataaagaa ttatttttaa aagcgacaat attaaatacg acgcatttat
     8821 ttaggagtgg caaacgtatg aatgggaaaa aggcgaatac gataaacaga tacaaatatt
     8881 ttcatcatgt cgatcatcaa aaaattcaac aaagttctaa aaagacgcta tgggcatcac
     8941 ttatcatcac actattattt acagtgattg aatttgttgg aggtttagta tctaattcat
     9001 tagcattgct gtcagactca tttcatatgc ttagtgatgt attagcactc ggtttgtcaa
     9061 tgttggcaat ttattttgct agtaagaagc cgactgcacg ctacacattc ggatatttaa
     9121 gatttgagat attagcggca tttttaaatg gattagcatt aattgtgatt tcaatttgga
     9181 ttttatatga agctattgta cgtattattt atccgcaacc cattgaaagt ggcattatgt
     9241 ttatgattgc tagtattggt ttactcgtca atattatttt gactgttatc cttgtaaggt
     9301 ctttaaaaca agaagacaat atcaatattc aaagtgcatt atggcatttc atgggagact
     9361 tattgaactc tattggtgtc atcgttgcag ttgtattgat ttactttaca ggatggcgca
     9421 tcatcgaccc aatcattagt attgtaattt cactcatcat tttacgtggt ggttataaaa
     9481 ttacgcgtaa tgcgtggtta attttaatgg aaagtgtgcc tcaacatttg gatactgatc
     9541 aaattatggc agatattaaa aacatagatg gcatattaga tgtacatgaa tttcatttgt
     9601 ggagtattac aacagagcat tattcattaa gcgcccatgt tgtgttagat aaaaaatatg
     9661 agggtgatga ttatcaagcg attgatcaag tatcatcatt gttgaaagaa aaatatggca
     9721 ttgcacattc aacgttgcaa attgaaaact tgcaattgaa tccattagat gagccatact
     9781 tcgacaaatt aacataaata aaacattgta gcgcctaaaa cattaatcta tgtcataggc
     9841 gcacgtttcg ttttatactt atgttgcatc atctaaatga ttttcgtcga tgtctttgat
     9901 ggtatctaca tctaacacga catctttagg tttcaaaata tgaatatgtt tttcatcatt
     9961 tgtatgtaaa atgcgttcta tgatgtacct ttgaccggtc attgtttcta cagcaatctt
    10021 tttgtttctt gctaaacttg ctacgacaga ttctttatcc ataatgatag ccccctatat
    10081 atatgtttat ttacttatac cctaacatta tttttatact ctttgaaaat atattttaca
    10141 gaattttatc taaatattta aaaaaatagc ttaatatcct tgtaatctga taataattat
    10201 agtaatattt tttcaaccat tgttatagga ggtcttatta atgacattat ttttattaga
    10261 agctaacaat cttgattttg catcaacgaa agaagaacta gaagcaaagg cagcatcact
    10321 atctacgaag acaattccaa cattaattga agtacaagct actgaaaatt taactcatgg
    10381 ttattttatt gtggaagcaa atgacgaagc agaagctaaa caatttttaa cagaagcaga
    10441 tattagtatt caattagtca aagaagtacg cttagttggt aaagatttag atgaagttaa
    10501 aaatggtgat gcacatgttg attaccttgt aacttggaac attccggaag gcattacgat
    10561 ggatcaatat ttagcacgta aaaagaaaaa ttctgttcat tatgaagaag tgccagaagt
    10621 tgaatttaaa cgcacatatg tatgtgaaga tatgtctaaa tgtatttgtt tatacaacgc
    10681 acctgatgaa gaagcggtac gtcgcgcgcg caaagcagtt gatacaccga ttgatggcat
    10741 cgaaaaactt taataagaca acaagttgat gagatatatg tatataggtt tgacgtggat
    10801 tttgattgca gttaattaga atagctcaat gctataaatg taagtagttg atatgaagaa
    10861 actaatgaac taaatgcaag tattgtctaa aacaatcatt ttattgaaat ttagtagagc
    10921 tgaaattaat ataacgtcgt taattgaata acgcttatgt tataagagca ctcataccaa
    10981 accataatca tctatagata taacaattca cgatataagg gctgtgtttg gcatagccct
    11041 ttagatatac acttaattcc tattaaaata gtagggatta aaagggggct tgtcatgatt
    11101 aaaattcaac aattacaaca tcactttgga tcacataaag taattcataa ctttaatttg
    11161 gacattagca agggagaaat agtcactttc ataggaaaaa gtggttgcgg aaagtctact
    11221 ttactcaata tcatcggtgg atttattcat ccatcatctg gtcgtgtcat tattgataac
    11281 gaaattaaac aacagccgtc tccagattgt ttaatgctat ttcaacatca taatctgctg
    11341 ccatggaaaa cgattaatga caacattagg attggattcc aacagaaaat tagtgatgaa
    11401 gagattaacg cacagcttaa attagttgat ttagaaggca ggggaaagca ttttcccgag
    11461 caactgtccg ggggtatgaa acaacgtgtg gcactatgtc gagcgcatgt gcataagcct
    11521 aacgttatat tgatggatga gccattaggt gcattagatg catttacacg ttataaactt
    11581 caggatcaac tagtgcaact aaaacataaa acgcaatcaa ctattatttt agtgacgcat
    11641 gacattgatg aagctattta tctttccgac cgcattgttc tgttaggtga agggtgcaat
    11701 attatttctc aatatgaaat tacagcatca catccacgca gtcgtaatga tagccaccta
    11761 cttaagattc gtaatgaaat tatggaaaca tttgcattga atcatcatca agttgaacct
    11821 gaatattatt tataaggagt gagtgacgat gaaaaggtta agcataatcg tcatcattgg
    11881 aatctttata attacaggat gcgattggca aaggacgtct aaagaaccgt ctaaaaatgc
    11941 ccaaaatcag caagtgatta aaattggata tttgccgatt acacattcag ctaatttgat
    12001 gatgactaaa aaattattat cacaatacaa tcatccgaaa tataaactag aattagttaa
    12061 attcaataat tggccagatt taatggacgc attaaacagt ggtcgtattg atggtgcatc
    12121 aactttaata gagctagcga tgaaatcaaa acagaagggc tcaaatataa aggctgtggc
    12181 attgggccat catgaaggca atgtcattat gggacaaaaa ggtatgcact taaatgaatt
    12241 taataataat ggcgatgatt accattttgg tataccacat cgttattcaa cacattatct
    12301 tttacttgag gaattacgta aacaattaaa gattaaaccg gggcatttta gctatcatga
    12361 aatgtcgcca gcagaaatgc cagccgcatt gagtgaacac agaattacag ggtattctgt
    12421 agccgaacca ttcggtgcac tgggtgaaaa gttaggcaaa ggtaagactt tgaaacatgg
    12481 tgatgacgtt atacctgatg cgtattgctg tgtgctagta ctgagagggg aattgcttga
    12541 tcaacacaag gatgtagcgc aagcatttgt acaagattat aaaaagtctg gctttaaaat
    12601 gaatgatcgc aagcaaagtg tagacattat gacgcatcat tttaaacaaa gtcgtgacgt
    12661 tttaacacag tcagcggcat ggacatccta tggtgattta acaattaagc catccggcta
    12721 tcaagaaatt acgacattgg taaaacaaca tcatttgttt aatccacctg catatgatga
    12781 ctttgttgaa ccgtcattgt ataaggaggc atcgcgttca tgacacgtcc cacaaataac
    12841 aaatttatat tacctattat cacatttatt attttcttag gcatttggga aatggtcatt
    12901 attattgggc attaccaacc tgtattgtta cctggtcctg ttcttgtagg aaaaagtata
    12961 tggactttca ttgttactgg agaaattttc caacatttag caattagttt atggagattt
    13021 gtagcgggct ttgttgtcgc attgttggtt gctattccat tgggcttctt gcttggaagg
    13081 aatcgttggc tatacaacgc tatcgaaccg ctatttcaat tgattaggcc gatatctccg
    13141 atagcatggg caccatttgt tgttctatgg tttggtattg gtagtttgcc agcgattgcg
    13201 attattttta tcgctgcttt tttcccaatt gtgttcaata ctattaaagg cgttagagac
    13261 attgaacctc aatatttaaa aatagcggca aatttaaatt taactgggtg gtcattgtat
    13321 cgcaatatat tatttcccgg ggcatttaaa caaatcatgg ctgggataca tatggcggta
    13381 ggaacaagtt ggatattttt agtttctggt gaaatgattg gtgcacaatc gggattaggt
    13441 tttttaatcg ttgatgcacg aaatatgttg aacttagaag atgttttagc agcaatattc
    13501 tttatcggat tatttggttt tattattgat cgattcatta gttatattga gcagtttata
    13561 cttagaagat ttggtgaata aggagagatg atgatgactt tagaaacgct tatcaaagaa
    13621 caattagatc ctcatttagt agaagttgat gaagggacgt attatccgag aacatttatt
    13681 caacaattat ttgtagatgg ttatttcggt gaggcggcat tgagaaaaaa tgctgaagta
    13741 atcgaagctg tatcgcagtc ttgtttgaca acaggatttt gtttatggtg ccaattagct
    13801 ttttcaacgt atttagaaaa tgccacgcag ccacatttaa ataatgactt acaacagcaa
    13861 ttgttatctg gagaaatatt aggtgctacc ggattgtcta atccgatgaa gtcatttaat
    13921 gatttagaaa agttgaacct tgaacacact tatgttgatg gacaattggt tgtcagtgga
    13981 cgtatgccag ctgtaagtaa tattcaagaa gaccattatt ttggtgcgat ttcgaaacat
    14041 gaatcatcag atgaatttgt catgttcatt ctacgtgcca atcaagatgg tatcactctc
    14101 gttgaaaaaa caaatttttt aggggtcaac gggtcagcaa cgtatcaaat cacattgaat
    14161 taagtcgtag tgccacaatc acaaatcatc acgcatgatg cgaagcagtt tgcggcaact
    14221 attcgcccgc aatttattgc ttaccaaatt ccaataggat taggctcaat taaaagttct
    14281 ttagagttaa ttgatgcatt ttcaaatgcg caaaacggaa taaatcaata tttagagtat
    14341 gatgttgaag cttttaaaaa acgttatcgt caacttagag aggaatatta tgcaatatta
    14401 gatgacggta acttaacttc acatttaagt gaattaatat cattgaagaa gaacattggc
    14461 tatttattgt tagatgtaaa tcaagcttct gttgtcaatg gtggttcaag agcgtacaca
    14521 ccatattcgc cacaagttcg caagttaaaa gaaggattct tcttcgcagc attgacacca
    14581 acattaagac atttaggtaa acttgaggca gagttgaagg ggtaagtatg atatgctgat
    14641 ctgttgttta gatgcgtttg ttgaaacatt ttttaaaata atataaatct tagtttataa
    14701 acattttctg tttatttgtt atatcctttt aactaggaaa atatacattt cgtaataata
    14761 ataatcgtta tcattgaaaa agtgtttata aggtgtataa tgaaaatgtg aacaattaat
    14821 gaacttctta ttttaaagaa ggtgaatact atagatacgc atactaaaga acaacaattc
    14881 tcgaatctag taagatctta tcgtaaagaa tacgtgggta aaggacccaa tagtattcga
    14941 gtgtcgttta aagataattg ggcgattgca catatgacag gtgttttgag taaagttgag
    15001 agtttttacc taaacgacaa acgcaatgaa tcgatgctcc attatacacg cacagagaag
    15061 attaaacaga tgtataaaga aatagatgta aatgagatgg aaagtcttgt aggcgctaag
    15121 ttcgtaaaat tatttacaga tattgatttg aatgatgatg aagtcatttc aatatttgtt
    15181 ttcgataagt caatagaata agtgttgctg gtgtaaggta cacggtgctg tttgctaact
    15241 tcgctttgaa tttaacaata attcaagggg gtggtatgtc aaacggtgcc gtttttttgt
    15301 catattttta aaacaagcaa catgcaacac gtactttaag gaagtcaaaa tttatcattt
    15361 aggagagatg gatatgaaaa tcgtagcgtt atttccagaa gcagtagaag gtcaagaaaa
    15421 tcaattactt aatactaaaa aagcattagg attaaaaaca tttttagagg aaagaggaca
    15481 tgagttcatt atattagcag ataatggtga agacttagat aaacatttac cagatatgga
    15541 tgtgattatt agtgcgccat tttatcctgc atatatgact cgtgaacgta ttgaaaaagc
    15601 accgaacttg aaattagcaa ttacagcagg tgtaggatct gaccatgtag atttagcggc
    15661 agcaagtgaa cacaatattg gtgtcgttga agttacagga agtaatacag ttagtgtggc
    15721 agaacatgcg gttatggatt tattaatact tcttagaaac tatgaagaag gtcatcgtca
    15781 atcagtagaa ggtgaatgga acttgtctca agtaggtaat catgcgcatg aattacaaca
    15841 caaaacaatt ggtatttttg gatttggtcg aattggacaa cttgttgctg aaagattagc
    15901 gccatttaat gtaacattac aacactatga tccaatcaat caacaagacc ataaattgtc
    15961 taaatttgta agctttgatg aacttgtttc aacaagtgat gcgattacaa ttcatgcacc
    16021 attaacacca gaaactgata acttatttga taaagatgtt ttaagtcgta tgaaaaaaca
    16081 cagttattta gtgaatactg cacgtggtaa aattgtaaat cgcgatgcgt tagttgaagc
    16141 gttagcatcc gagcatttac aaggatatgc tggtgatgtt tggtatccac aacctgcacc
    16201 tgctgatcat ccatggagaa caatgcctag aaatgctatg acagttcact attcaggtat
    16261 gactttagaa gcacaaaaac gtattgaaga tggagttaaa gatattttag agcgtttctt
    16321 caatcatgaa cctttccaag ataaagatat tattgttgca agtggtcgta ttgctagtaa
    16381 aagttataca gctaaataga ataaggatgc tgggctagcg attaacgctt tcaattttat
    16441 ataaatgaat tatataagca ctactgctgt tgtaaagatg gcagtagttt ttttatgatt
    16501 acatctaagt atagtcacgg ctatgttagg acaatgattt aacatttacg cacatatgtg
    16561 ttcacttacg caattattga taaatttcat tcatttggaa taatatatag tcatatagtg
    16621 ctaatttttt tgaggcatta tatgcaagat gcattgtaaa gtcgatgcat ttgtttgtta
    16681 aataactttg tataactaaa aattctttgt ttcaacgtat aatctaataa tagattttat
    16741 ataaatggta gtgtgtatat atgtggatag gggtgtagtt attaatgaaa cgcttaagta
    16801 cgactttgaa agtacgattg attagcaatt ttttacagct aattattacg acagcattta
    16861 taccgtttat agcactatat ttaacagata tgttaagtca atcaattgtc ggtatatatc
    16921 ttgttggttt agtggttcta aaatttccat tgtccattat atctggttac cttattgaga
    16981 tatttccgaa aaagttgcta gtacttattt atcaagcgac gatggtgata atgcttgtgt
    17041 tcatgggcat atttgggtca catcaattgt ggcaaattat tggtttttgt gttgcatatg
    17101 ccatatttac aatcgtttgg ggattacaat ttccagttat ggacacatta attatggatg
    17161 caattaccga agacgtggaa cattatattt acaagattag ctattggatg acgaacttat
    17221 cggtagctat tggggcattg ttaggcggct tgatgtatgg ctacagtatg ttactacttt
    17281 tcttaatagc agcttgtata tttttaattg tactctttat tttatatatt tggttacctc
    17341 aagaccgaaa tcaagtaaag caaagtgatg acaagaggca tgcaagtcgt tatcaaaaat
    17401 tacaaataat gaatatattt cgcagttata aattagtttt gaaagaccgt aattatatgt
    17461 tattgatttc ggggttcagt atcatcatga tgggtgaatt ttcaatctcc tcatatattg
    17521 ctattagact aaagaatcag tttgaaacaa taagtatagg ttcatatgat attacaggtg
    17581 ctaagatgtt agcaatcttg ctaatgatta atacggtcgt cgtcatttta ctcacgtatt
    17641 caatctcgaa agttgtattg aaaatagatt ttaaaaaagc tttaatcact ggtttgctga
    17701 tttatattgt tggctatagt ggtctaacct atcttaatca gtttggctta ttagttgttt
    17761 ttatgataat tgcgactgta ggtgaaatta tttattcgcc tatagtttca gaacaacgct
    17821 ttaaaattat tcctaaagct aaaagaggaa catatagtgc agttaatgca ttaggtattc
    17881 atttttcaga aacactagct aggttaggga ttgtgttggg tgctttctta acgtcattac
    17941 aaatgggact gtatatgttt atcgttttaa caattggtgc tagcatgctt gttgctggtg
    18001 tattcggggg acaaaaacaa gtgaatacaa attgaattta tttaaatatt tattttatgc
    18061 atctttttat attgaaaacc tattaattcg gtcgtaatta aaatagagat aaatttatta
    18121 agaatttgtt aaaatttaag gataacaatt gttttatgcg agttgtttgt ttaatataaa
    18181 cttgcttcat ggatattttg taacaacaat attgaatttg caatgagatg tgaacaacag
    18241 aaagtgatta acgagttgta gtgtactatt tatcgaagat gtatgtaatt tacattgaaa
    18301 aatataccaa ttgtaacgat gatacaagat gtattttagg gtagaatata tcgctcatat
    18361 gttgcgaatg gtgttttata ttaatcatta cataacgagt taattaacat ttcgtcggtt
    18421 atcatatcat catgcaattt cagtaagtaa tgagtattat gatatgaaag aaggactttt
    18481 tatgattatg ggtaatttga gatttcagca ggaatatttt cgtatataca aaaataatac
    18541 agaatcaacg acacaccgta atgcgtattg ggttaaactc gctaaaaatg ttgaagctac
    18601 taaaatgatg tatgcattat cgacaattgt gcaacaacat gcatctataa gacatttttt
    18661 tgatgttact accgatgaca atttaacaat gatacttcat gaatttctgc cttttattga
    18721 gataaaacaa gttccaactt cttccgcaaa ctatgattta gaagcttttt ttaagcaaga
    18781 attaagtact taccatttta atgattcacc tttattcaaa tttaaattgt ttcagttcgc
    18841 tgatgctgca tatatactat tagattttca tgtgtccatt ttcgatgata gtcaaattga
    18901 tatttttctt gatgatttat gcaatgcata tcgtggcaat actgttatta acaatactcg
    18961 acagcatgca catttaaata gaaatgatga taagcacaat caagatgcat cgcatatagc
    19021 attagactca aactattttc ggttagagaa taactctgac atccatattg atagttattt
    19081 accaattaag catccatttg agcaagcttt atatcaaacg tatttgattg atgatatgac
    19141 atcaatagat atggcatcgt tggctgttag tgtgtattta gctaatcata taatgagtca
    19201 acaacatgat gtcacattag gtatacatgt accatcacat ttaccaaatg atttacacgg
    19261 aaatattgtg ccgttaacgt taacaatcga tgcaaaagat gtatgtcaac gttttacaac
    19321 agattttaat aaatgtgtgt tgcaaaatat gtcgcaatta cagtgcgcga attcttcgct
    19381 ttcactagag actatttttc attgttatca tcatatgatg tcttgttgta atgatgttat
    19441 tgaggatgta catcaaatac atgatgcaca tacatcttta gcggatattg aaatttttcc
    19501 acatcaacac gggttcaaaa ttatatataa cagtgcagca tatgatttgc tctcaatcga
    19561 gacgctgagt gacttagttc gaaatattta tttgcaaatt actgaagaaa atggaaataa
    19621 acgaacaact gtagatgaac ttaatttgat gacagaacgt gatattcaat tatatgacga
    19681 tatcaatgta agtttgcctg agatagatga tgcgcaaaca gttgttacct tatttgagca
    19741 acaagttgaa gcaacgccga atcatgtcgc tgtgcaattt gacggagtgt ttataacata
    19801 tcaaacattg aatgcacgcg cgaatgattt agcacaccgt ttgagaaacc agtatggtgt
    19861 tgaacctaat gatcgtgtcg ctgtcatagc tgaaaaaagt attgagatga taatagcgat
    19921 gataggtgtg ttgaaagctg gtggggctta cgtgccaatt gatccgaact atccaagtga
    19981 tcgtcaggag tacattttaa aagatgcaac gcctaaagtt gtaataacgt accaagcttt
    20041 atatgaaaat ggtaaacaaa atattaatca cattgatttg aataagatag cgtggaaaaa
    20101 tattgataat ctttctaaat gtaacacgtt agaagatcat gcttatgtta tttacacgtc
    20161 ggggacaact ggtaacccaa aaggtacact aattccgcac cgaggtattg ttcgcttggt
    20221 ccatcaaaat cattatgtac cattaaatga agagacgacg attttgttat caggaactat
    20281 agcctttgat gctgcaacat ttgaaatata tggtgcattg ctcaatggtg gaaagctgat
    20341 tgttgctaaa aaagaacaat tattaaatcc aatagcgtta gaacaattaa tcaatgaaaa
    20401 tgacgttaat actatgtggt taacctcctc attatttaat cagattgcta gtgaacgaat
    20461 agaagtattg gtaccgttaa agtacttatt aattggtgga gaagtattga atgctaagtg
    20521 ggtggatttg cttaatcaaa aaccgaagca tcctcaaatt attaatggtt atggaccaac
    20581 tgaaaataca acatttacaa cgacgtataa tatacctaac aaagttccaa atcgtattcc
    20641 tattggtaaa ccgattcttg gtactcatgt ttatatcatg caaggcgagt gtcgatgtgg
    20701 cgttggtatt cctggagaat tatgtacaag tggctttggg ttagctgcag gttatttaaa
    20761 tcagccagaa ttgacagcag ataaatttat caaagattca aatataaatc agctgatgta
    20821 tagaagtggt gatatcgttc gtctattacc cgatggcaac atagattatt tatatcgaaa
    20881 ggacaaacaa gttaagattc gagggtttag gattgagttg tcagaggttg agcatgcgct
    20941 cgagcgtata caaggtatta ataaagcagt tgttattgtt caaaatcatg atcaagatca
    21001 gtatatcgtt gcttattatg aagcgatgca tacattatca cataataaga ttaaatcaca
    21061 attacgtatg accttaccgg agtacatgat accagttaat ttcatgcata ttgagcaaat
    21121 tcctattact attaatggga aattagataa gaaggcattg cctatcatgg actatgtcga
    21181 tacggatgcc tatgtagcac cgagtacaga taccgaacac ttgctatgcc aaatttttgc
    21241 agatatttta catgtgaatc aagtaggtat tcatgataat ttctttgaat taggtggcca
    21301 ttcattaaaa gcaacgttag tggtgaatcg gatagaggca tctactggga aacgattaca
    21361 aattggtgat ttattacaaa agccaactgt atttgaacta gcacaagcga ttgctaaggt
    21421 tcaagaacaa aactatgaag tgattccaga agctatagtt aaagatgatt atgtgctgag
    21481 ctctgcacaa aagcgtatgt atttattatg gaaatcaaac cataaagata cggtgtataa
    21541 cgtacctttt ttatggcggt tatcatcagg acttaatgta gctcaattgc aacaagcagt
    21601 tcagcatttg atagcgcgac atgagattct acgaacacaa tatattgttg tagatgatga
    21661 ggttcgacaa cgtattgtgg cagatgttgt agctgacttt gaagaagtta acacgcattt
    21721 tacggatgaa caagaaatca tgcgccaatt tgtggcacct tttaatttgg aaaagccaag
    21781 tcaaattaga gtgagataca ttagaagtcc cttacatgca tacctgttta tagatacaca
    21841 tcatatcatt aatgacggta tgagtaatat acaattaatg aatgatctta acgcacttta
    21901 tcaacataaa ttattgttac cacttaaatt gcaatataaa gactatagtg agtggatgtc
    21961 gcatcgtgat atgacgaaac atagacaata ttggttatct caattcaaag atgaagtacc
    22021 tattttaagc ttaccgacag actatgttag accaaatatt aaaacgacaa atggagcaat
    22081 gatgtcattt acaatgaatc aacaaatgag acagctactt caaaagtatg tagaaaagca
    22141 tcaaattact gattttatgt tctttatgag tgtggtcatg acgttgttaa gcagatatgc
    22201 tcgaaaagat gatgttgttg tcggtagtgt gatgagtgcg cgtatgcata aaggcacgga
    22261 gcaaatgcta ggcatgtttg ctaatacgtt ggtatataga gggcaaccgt cacctgataa
    22321 aatgtggaca cagtttttac aagaggttaa ggaaatgagt ttggaggcat acgagcatca
    22381 agaataccca ttcgaatgtt tagtaaatga cttagatcaa tcacatgatg cctcacggaa
    22441 tccattattt gatgtcatgt tagtactaca aaacaatgaa acgaatcatg ctcattttgg
    22501 gcatagtaaa ttaacacaca ttcaacccaa atcagtgacg gcgaaatttg atttatcttt
    22561 catcattgaa gaagatcgcg atgactatat aatcaatatc gagtataata ccgatttata
    22621 tcactcagaa acagttcgtc acatgggtaa tcaatgtatg attatgattg attatatttt
    22681 gaagcatcaa gatacactac aaatttgtga tataccaaac ggcacggagg aacttctaaa
    22741 ttgggtcaat acgcatgtta acgatcgaat gcttaatgtc ccgggaaata aatctatcat
    22801 aagttacttt aatgaagctg tctcacgaca aggtaatcat gttgcgctag tcatgaatga
    22861 tttgacaatg acgtatgaaa cattacacaa ctatgtggat gccattgcgc acatgctcct
    22921 atcaaatggt gtgggcaatg gtcaacgggt tgccttgttt acagaacgta gttttgaaat
    22981 gattgcggcg atgttggcga cagttaaagt aggtgcatct tatataccta tcgatattga
    23041 ttttccgaat aaacgacaag gtgcaatttt ggaggatgct aaagtaactg cagtcatgtc
    23101 ttatggcgtt gaaattgaaa cgacattacc agtcattcaa ttggaaaatg ctaaaggctt
    23161 tgttgaatca aaggaaaatg aacaatatga tgatttacat ggcaatcaac ttgaaaacac
    23221 agcgatgtta gataatgaga tgtatgctat ttacacatct ggtacgaccg ggatgcctaa
    23281 aggggttgcc atacgacaac gaaatttgtt gaatttagtg catgcatggt caactgaatt
    23341 gcaattaggc aacaatgaag tatttttgca acatgcaaat attgtttttg atgcatcagt
    23401 tatggagatt tattgttgtt tgttaaatgg tcatacgctt gtgattccag atagagagga
    23461 acgtgttaat ccagcacagt tacaacaact cattaataag catcgtgtga cggtggcgtc
    23521 gattccgtta cagatgtgta gtgttatgga agacttttat attgaaaagt tgattacagg
    23581 cggggcaact agtacggcat cctttgttaa atatattgag aagcattgtg gcacgtattt
    23641 caatgcctat ggaccatctg agtcaacagt catcacatcg tattggtcac atcattgtgg
    23701 tgatttgata cctgagacga ttccaattgg caaaccctta tctaacatcc aagtgtatat
    23761 tatgtcagat ggtttgttat gcggtattgg tatgccaggc gagttgtgta ttgcaggtga
    23821 tagtttagcg ataggatata ttaaccgtcc agaattaatg gctgataaat ggcaaaataa
    23881 tccatttggt aaaggaaagt tgtatcatag tggtgattta gcacgttata catctgatgg
    23941 tcaaattgaa tttttaggaa gaatagataa acaagtgaaa gttaacgggt accgtattga
    24001 acttgatgaa attgaaaatg caatattagc tattcgtggt atatctgatt gtgttgtaac
    24061 agtaagtcac tttgatacgc atgatatatt gaatgcttat tatgtcggag agcaacaagt
    24121 ggagcaggat ttgaagcaat atttaaatga tcatctgcct aagtatatga ttcctaagac
    24181 tataacgcat atcgattgta tgccattaac cacgaatgac aaggtggata ctacgcgttt
    24241 gccaaatcca tcacctatac aacagtctaa taaagtgtat agcgaaccct ctaatgaaat
    24301 tgagcagaca tttgttgatg tatttggaga ggtattgaaa caaaatgatg tcggtgttga
    24361 cgatgatttc tttgaacttg gtggtaactc attagaggcg atgttagttg tctcgcattt
    24421 aaaacgattt ggccatcata tttcaatgca gacattatac caatataaaa ccgtgcgaca
    24481 gattgttaat tatatgtacc aaaatcaaca atcattagtt gcattaccgg ataatctttc
    24541 ggaattacaa aagattgtta tgtctcgtta taacttgggt attttagagg atagtctaag
    24601 tcatcgacct ctaggaaata cactattgac tggcgtgaca ggttttttag gtgcttatct
    24661 gattgaagca ctacaaggat acagtcatcg catttattgt ttcatacgtg ctgataatga
    24721 ggaaatagca tggtataagt tgatgacgaa tttaaatgat tatttttcag aagagacggt
    24781 tgaaatgatg ttatcaaaca ttgaagtcat tgttggtgat ttcgagtgta tggatgatgt
    24841 tgttttacca gaaaacatgg atacgattat tcatgcaggt gctcgtacag atcattttgg
    24901 tgatgatgat gaatttgaaa aagtaaatgt tcaaggtact gttgatgcca tacgtttggc
    24961 acaacaacat catgcaaggt taatatatgt gtctacgata agtgtgggaa cttattttga
    25021 tatagacaca gaagatgtga cattttcaga agcggatgtc tataaaggac aactactaac
    25081 atcaccatat acacggagta aattttatag tgaattaaaa gtattagaag ctgtaaataa
    25141 tggcttagat ggtcggattg tacgtgttgg taatttgacg agtccttaca atggaagatg
    25201 gcatatgaga aatataaaga ctaaccgttt ttcaatggta atgaatgatt tgttacaact
    25261 ggattgtatc ggggttagcc tggctgaaat gcctgtagat ttttcttttg tggatacgac
    25321 tgcaagacaa attgtcgcat tagcacaggt caacacacca caaatcattt accatgtgct
    25381 atcacctaat aaaatgccgg tgaaatcttt gttagaatgc gttaagcgca aagaaattga
    25441 actcgtcagc gatgaatcat ttaatgaaat tttacagaaa caagacatgt acgaaacgat
    25501 tggattaact agtgttgacc gtgaacaaca actagcaatg atagatacaa cattaacatt
    25561 aaaaataatg aatcacatca gtgaaaaatg gccaacgata actaacaatt ggctgcatca
    25621 ttgggcgcaa tatatcaaaa caatattcaa taagtaagta gggaaagtta tgacagtatt
    25681 tgtaatgcaa ttacagagta acttgaaaag tattgaagaa ttaatatcac aaagtcgttg
    25741 gtcatataaa aaaccacgta cagtcaacta tagatacaat caagataaac tcatgcacag
    25801 attgggagat attttagtgc aatatggaat tcaacatgac acaggtttat taccacatga
    25861 atggcattat cacatttcgc cacgaggtaa ggcagatatt gttcaacaca atcgtgatgg
    25921 acagcccatc tatgtgagct tatcatatag ttatccttat atcgtgtgtg ttgtcgataa
    25981 agaaccagtt ggtatagata tcgaaaagat atcacaacgt ttagactggc gtacgttagt
    26041 gacgtgtttc tctacaaacg aagcacatca aatatgtagt ttaaatgatt tttatcaaat
    26101 atggacacaa aaagaaagtt ttacaaaatt gattggtgaa ggtttaatca aaggattgga
    26161 catttatgat atgacacaat cacactttta tcaatcacgt gaagtgaagt tcaaacaatt
    26221 tatttttgat cagtttatgg tacaggtatg tttcttagga caggcaccct ggggttataa
    26281 aaaagtgtct gtatttcagt tattgagtag ttaatatgac gtctgacagt atcattgcgc
    26341 agtgatttta aactatactg cctaaatgtt tactgttaga tgttttttta ttggaaaaat
    26401 gaaaggtgaa tacacgatgg catcattcaa aatcatgacg agattaaagt aagtgataag
    26461 gtgaaaatat actgagcaat attgaaatta ccgaaaataa tagtttgtta cgacgcattt
    26521 gagactttaa tgctcacgta agttggctgg aatcatatca tgtcagttaa taaaataacg
    26581 tgagcatcaa tcatcatact caaacgatga tttaaaactc acgttaatgt tagtcagtgt
    26641 attaatttaa tttatagcga tagacaaaat gttgtcgcac taattcaatt gtcatcatcc
    26701 agacgatatg gccaaagaat tctgacagat gctcttggaa tggttgatcc cacacagcag
    26761 gtacagtatg catgattggc ataatgataa ggtggaataa tacccaaata gcaataccaa
    26821 atacagcacc ttgtcccatc gctaagtaag cgtatttttt aactaatatg cagtaaataa
    26881 ttgcaatgac gatagaaaaa ctaaagtgga caataaagct tacccaaggc aattccatat
    26941 ttgaaaatgt atatgtttga tgcgtaaact cactactaaa tcctaattgt tgcaataact
    27001 cttgaggtgg gtttgttgca ttacgttctg gtgtgcgagg tggaaacatg acctcccaac
    27061 ctaattttac aattccagat aacaagccac cgataattcc agcataaatg aatataccca
    27121 tttgtcgttt cgtcaactta aggactcctc tctatttact atcctcatta tagtattttg
    27181 tgaaaataat cacaatgaaa agctttgcaa aacttaaaat attttgataa acaaatttgt
    27241 aaaaggttat tagtttttta catctcatat tgtatcatac tgtattcgga attattgacg
    27301 agatgaacgg taaataaatg tttcagcgca atggataaac gaactggaat catcgcgtgt
    27361 caaaacttgt tagtccttaa tttcatggtt atgaattaag gattgttgtg ccaacaaaat
    27421 cattattgta aatagattcg ataatatttg gcttgtttcc tgatgcaatg ataactttag
    27481 gacagccatt ttcaatcgca tttttagcat caagcacttt gggaatcata cctccataaa
    27541 tatcaccatg ctcaatatac tgattaatat cacctaatgg tagatgaggt ataacaacat
    27601 cattgatgag tacacctgca atattactta atacataaat aggtgctttt aatgatgatg
    27661 caataaaata ggcaagcgtg tcagcattaa tattgtaaaa ttctccatca tggttattga
    27721 aaccaatcga attaatgata ggtacaaatt tatcacatat atactgtaaa gcatccttat
    27781 ttaaagcggt cggaactccg acatatccat attgttgatc aaaagatgta atttcaaaca
    27841 gctgtgcatc tacaccacat aagcctattg cagaacattg gtgctggtta aattgagcta
    27901 ctaatgccgt gttaacgtct gcaatgagcg tgcgtttagt aatcgtcatg gttgctttat
    27961 cagtcactct taggccatta acaaagtgtg gctcgatttg ctggtttgat aatgcatcat
    28021 taataaaagg gccaccgcca tgaacgataa tagggtagat gttgtttgat cgtaaatgct
    28081 taatattgtt aataattgat ggatgcatgt cactaagtgt actgccacca attttaatga
    28141 caataaattt catctaacca acaccacctt atgttcgata tgatgcgtta atacgcacat
    28201 aatcatagga taaatcacaa ccgtatgcag tcgctgtagc gttacctaaa ccaagctgaa
    28261 cgtcaattgt gacatgttca tgagttaatg tattcgacat agcttgctca tcaaatagta
    28321 cagccatacc tttatcaacg acaggtattt ggttcagttg aacatatgtg cagttaggat
    28381 caatttcaca tccgctatag ccaatagctg taatgattcg accaaaattg gcatcttcgc
    28441 caaaaatagc tgattttact agatttgaac ttacgatagt tttaccgatt tttcttgcat
    28501 ctgatattga cttagcacct gacacattga cgctgattaa ctttgttgcg ccttcgccat
    28561 ctctagctat agctttagct aaaaatgtac agacaaaatt gaatgcatca acaaatgttt
    28621 cccattgtgg atggtcttga ttaagtattt ggtgttcaac ttggtgattt gccatgacta
    28681 ataccatgtc atttgtactt gtatcgccat caacagtaat catattaaat gtatggtcag
    28741 tcgaagattt taataattga tgaagtgtat tcgattcaat cgctgcatcg gttgttataa
    28801 aagcaagcat ggtagccata tttgggtgaa tcatacctga acctttggcg ctaccaccaa
    28861 ttgtaacggt tttaccatcg atttttagtg atacagcgat atgttttgta catgtatcag
    28921 ttgttaaaat tgcctcgtta aacgcacctg gcgttgtgta cttagtatcc ttaatatgat
    28981 cggtcccatt cttaatttta tccataggca aatattcacc aataacccca gttgaagcaa
    29041 cagcaacatg ctcagatggt atttgaagtt gttgagcaac ccatgtttgt gtttgtcgtg
    29101 catcatctat gccttgttga ccggtacaag aatttgcatt agctgaatta acaacaagtg
    29161 cttgtaattt tcctttagac ttttgtaaag tgtcttcagt gacaataagt ggtgcagctt
    29221 taaactgatt taaagtatat acggcagctg cacttgccaa agacgatgag taaatccagc
    29281 caaagtcttt tttgttagcg cgtaaaccga tgtgcatacc accagccgtg aagccttgag
    29341 gtgtactaat atcgccatgt ttaataattg aaaagttata ttgttgtgat gtcgtttctt
    29401 gatgtttcat tctaacaccc cttatggata aactggtgat tgttttaggc cagtcgtaac
    29461 ttcaaaatta tataatatat ttaagttttg aatggcttga ccacttgccc ctttgacaag
    29521 gttatcaatc actgatacta aaattgctgt ttgcgttgtt tcatctacat agataccgat
    29581 atcgcagtag ttactaccga gtacttcttt tgtggttgga aaagtcccaa tatctctaat
    29641 tctgacaaat ggctgattag cataataaga ggtcattaat ttatgtaatg attcagttgt
    29701 atattcagat gataatttga cataaattgt tgataaaata cctcgtgtca ttggtacgag
    29761 atgtggtgta aatatgactg atacatcttg acccgcaacg atagataaat attgctcgat
    29821 ttccggtttg tgtttatggt ttccgattgc ataagcgctt agattttcat tcatttctga
    29881 aaaatgaaca cgttgtgata atgaacgacc agcacctgac acgccggtct tagcatcaat
    29941 aataatagat gacaaatcta ctattttttc gctaataagt ggatgtaatg ctaataatgt
    30001 tgctgtaggg aaacagccag gattagaaat gagcttcgtt ccattgttat caaacgattg
    30061 ccattctgaa atgctgtaaa tagcatgatt caaatcatct tgtgctgcag cagtttcttt
    30121 gtaatatgct tcatatattt cacgattctt aattctaaat gcgccagata aatcgataac
    30181 atgaatacct ttttctacta agggagggat acatgtttta cttacgggtg ctggtgtcgc
    30241 aaagaaaatt acatcacagt cattattgtc cactgtaagt gcttcgaaat gttgcataat
    30301 atgttgtaaa tgtggaaatg ttaatttcaa cggttcatct acttttgaat gtgagtagat
    30361 gtgtgcaatc gttatatgag gatgtgtttg taataatcga attaattcaa ttgcgccata
    30421 accgctaccg ccaacgatac ctactttaat catcatgaat ccctcctatg tcatatataa
    30481 atgattaatt gttaattttt aaaaacgtct tgaaaagctg caacaatttg attgatttcc
    30541 tctctatcaa tgactagagg tggagacaat cgaatgatag tacgatgcgt gtctttgcat
    30601 aagattccac gttgaatcag ttgatccaca aaaggtgcag catctgtgtt aagctctatg
    30661 cctataaata aaccacgacc tctaatttct ttaatactag gatgtttaag ttgtagcaac
    30721 gcttttaata aaaatgaacc taagcgttct gatcgttcaa ccagttgttc atctttaagt
    30781 acatcaagcg ctgccgtcga tattgcaatg gctaaagggt taccaccaaa tgttgaacca
    30841 tgtgtacctg gtgttagaac acgcatgaca tcattatttg caagtacagc agatacaggg
    30901 tataagccgc cacccaatgc cttacctaaa atataaatgt ctggaacgac ttgctcccat
    30961 tccatagcaa accatttccc agttctacca agaccaactt gaatttcatc tgcaatcaat
    31021 aatatttgat gtttatcaca tagttgacgc acagcttgaa tatatccttt cggtggtata
    31081 ttaacgccac cttcaccttg aattggttcc aaaataattg ctgctgtatt cggtgaaata
    31141 gcttgtgtta attgttcaat gtctccaaaa tctactgttg tagtgccttg aagtaggggg
    31201 tgaaatcctg ctttatatgc gtcgtggtta gatagtgata atgagccaag tgtacgaccg
    31261 tgaaaattgt tattcatagc gatgatttca acttgtccgt cagtaatgcc tttaacttca
    31321 gagccccatt ttctagcaat tttaatggct gcttcaacag cttcagtacc agagttaagg
    31381 gggagtactt tgtctttctt agcaagatga caaatttttt cttcccattt cccgagattg
    31441 tcactataaa ggacacgtga aatgatagac aactttgaag cttgttcagt catcgcttta
    31501 acaattgttg gatgacaatg gccttggttt gcaactgaaa aacctgagat gcaatctata
    31561 tattgtttgc catcagtatc ccaaactttg acacctttac ctttagaaat gacaagctta
    31621 agtggtgcat aattattaga gctataataa tcagttaatt caatgattga attcatcggt
    31681 ctccctcctt tatatttata caatttaaag aattaatatt aatatattac agttgttcaa
    31741 tataagtgtc aaatgtaaat ttaataatat aaagataagg ttgtgtttgt attaatgtga
    31801 tttgtaaaat gaaagtacta atattttttg tggataatca ttgatatgta tgtatacgat
    31861 tgcagtttaa tgtagtatga ataaaaaatg ccacaagcat atgtttaaca tatgactgtg
    31921 gcatagtatt atgcttgtgg aattttacga tgcttaattt taaaaataat gaagccgata
    31981 atgaaaccaa tcaaactgag aacaacccag cccataccaa tgtctgataa tggtaaatat
    32041 ttttggctga aattaatcaa agtttgtgag aatgatgtgc ttgaaatgaa ctctggacta
    32101 gcttttaatc catctactaa tgcagcaatc attgtaaaga aaatggtaca ttgataaata
    32161 atttttgagt gatggaattt gctactaaat aatgttagta caatcaaggc aattgctaat
    32221 ggatataaga acattaacac tgggactgag tacataataa tcttagttaa accaacattc
    32281 gcgaataaga acgaaataaa gcttacaact gttgcaatcg ctaggtaatt cattttaggg
    32341 aaaaggtgtt cgaatgtttc tgaaaatgcc gtaatcaaac cgatggctgt ttttaaacaa
    32401 gcaaccataa cgataagtga caacaggaca ataccgtagt tacctaagta gtattgcgta
    32461 atttgcgcta aggcaatacc accattttca ctaagtttga aatgaccaat acttaatgta
    32521 cccatgattg ctagtagggt ataaatgatc cccatcataa tgatactgat agtaccagac
    32581 ttaattgttt ctttagcgat atcagttgga ttttcgatac ctaacttttt aatcgttgca
    32641 acaatgatga taccaaatgc caatgacgct agcgcatcta aggtattgta tccatctaaa
    32701 aagccgttaa ataaggcatg tgattgatat tgtttactaa taggtgcatc agatatgcca
    32761 cctaatggat ggataaaagc aaataataaa ataattgcta ataatactaa gaataccgga
    32821 tttaaaaatt taccgatata ttctaaaatt cttgatggct ttctcgcaaa aaaccatgca
    32881 atcacaaaga agacgaagct aaaaataaat aaatataaag tgatttgctt tggtgataaa
    32941 aatggcgaaa atgcaatttc aaatgatgtc gttgccagtc taggtaaggc gaaaaatggt
    33001 ccgataacta gatataaggc aatcgtgaaa atgtaagcat atgttttatt aacacgcgat
    33061 gcaatttcaa ataaaccaga tgtctttgaa atgccaatag caatgatacc tagaaatggt
    33121 aagccaattg ctgtaattaa aaatcctaag ttagcgataa aaacgttaga accagcagct
    33181 tgacccaagt gtattgggaa gataagattg ccggcaccaa agaataaacc aaataacata
    33241 gaacctataa acatgttttc tttaaatgtt agtttcttct tcatatatgt atttgttctc
    33301 ctttttagaa cttataaaaa tctacttaat taagataata tcaaaaatta aacataaagt
    33361 gatacataat tttgcaataa atactaaatt ttttgaaaat tccggaataa atactgtttc
    33421 ttatgtagta ggggcgtaag ttaagaacga tttaacagat atagaatatg ttaaatatag
    33481 aaggttagac aacccgacgt attaatactt tgtgaaataa agatccccct caacatgatc
    33541 gaagggggga agtatatgtt tagttcaata tatttagaaa gtcgtttgta gttattgttt
    33601 gtcccatcaa tgggaataca ttatctattg gaaattgatg tagcgtttcg ttttgtgcac
    33661 tcatcatatc tgtaacaaaa aactgattgt agtttaattg ataggcatct cgcgctgtcg
    33721 tatctacgcc aatatgcgtt gcgacaccac caagaacaat cgtatcaatt cctcgacgtc
    33781 gcaattgtaa gtccaaatct gttcctacaa atgcactaaa atgtcgtttg tctatgacaa
    33841 aatcgtcatc tctcttgtct aataaatgat ggaaacgact gtagtcgtcg ccttcttttg
    33901 gtggtaatga gatcattgca tttggttgca atacatcttt accatcatag aaattcacgc
    33961 gaacaaaagc gataaagcca ttgttttttc taaaaacatc tattaattta ttagcgtttt
    34021 gaacgacatt ttcagctgta tgtggcgcat aatccatctt aagtatacct tcttgcaggt
    34081 cgattaacac taaagcggtt ttattaaaat taatcatctt atatcagagt ccttttcgtt
    34141 taataaagta ttcgcttaaa cttataccct gtatacgatg aaattattta ttttgttgtg
    34201 aaaaagcttt agcgatatcg atcagtttct tcggtgcgtc ttcgacagac attttgactt
    34261 cgacaaaatg catcacatcg ggatgaccat taattgcatt aaacgtgtct tgtaaatctt
    34321 ttgatgattc aacgtcatga atttcaacat ttttaccacc aaatacagct ggtaaagctt
    34381 tataatccca catgtgaatt tcattataag gttcatacat gccgtgaata agtcgttcta
    34441 ccgtatagcc gtcattatta atcacaaata ataccggttt aatatgctgt ctaatcatag
    34501 ttgaaatagc ttgaacagtt agttgcaatg agccatcacc aattaataat aagttacgac
    34561 gatctttgtc tgctaattgt gaacctaatg ttgcaggtaa tgtatagccg atagaacccc
    34621 ataacggttg ccctataaaa gtattgtttt tgtataatgc taaatcataa gcaccaaaga
    34681 atgatgtacc ttgatcagca ataatgacat catttggttt taagaaattt tgcatcattt
    34741 taaaataagt ttgttgtgtt aatggttctg tgccaacagt ataatcgggt gatgttggac
    34801 gatgataagc agggaacgat gcgttattcg tatatgaaat attggataac tgttttaaca
    34861 acgatggtag agatatttcg tcatttgtaa aatcgtcaat tttgatatta tgatgattta
    34921 acataacgac atcatcgata ttgaattggt atgaaaatcc tgctgttgct gaatctgtta
    34981 atttggctcc aatatttaaa attaaatcgc tgttgtccac ataatctcgt attttatctt
    35041 cggcaatttt cccatcgtaa atacccatat aatatggatt ttcctcatta aaagcacctt
    35101 ttcctaatga aagttgtgct actggtatct gtgtttgatt tacaaaatct tctaattctt
    35161 gatggaggtg aaaactgtta atttcatgtc cagtaatgat gataggctgc ttcgcttgat
    35221 gcagtttagt tgctaataac tctatatatg ttgatgcatc cgtatcttta gttgccgtca
    35281 cttcaaatgg tgtcggtatc tcaatttcag agattgcgac atcgattggt aaatgtaaat
    35341 gaactgggcg tctttcggcg attgctgtat taattaaacg tggtatttcg gttgttgcat
    35401 tttcaggtgt gatataacct tgtgcaacgg ttatatgtgc aaacattttt cggtagtcgt
    35461 caaatgtacc ttcaccaagt gagtgatgta catatttacc ggcttgttca acagcacgtg
    35521 tcggcgcacc tgtaatcgca atgacaggta tgcgttcagc atatgaacct gcgataccgt
    35581 tgacggcact taattcgcca acaccaaatg tagtaactaa tgcagcgagt ccattaagac
    35641 gggcataacc gtccgctgcg taacttgcgt ttaattcatt tgtatttcct acccaatcta
    35701 cattgggatt gctgataata tcgtctagaa aagcgagatt aaaatcacca ggaacaccaa
    35761 aaattttatc gacgcctgct cgatgaatag cgtcaattaa gtaagctcca atgcgttgtt
    35821 tcataaaaaa accacctttt ctctatattt acacttaaaa tgatagcctc gaatacagtg
    35881 taaggtcaag taagttgact ttgttgtgaa aacgctaacg atagtgaaca attaatattt
    35941 tttaggaaat gagtatgaag gggggcttta attgatgatc ttaaaattca aaatttaagg
    36001 ggagagcgaa ctatcaatga taccgcaaaa tacaatagca aagaaaaaac aggcgcgtca
    36061 ccttatgaag gtatacgcac ctgtttatag taagcattat ttagcttcaa ataattgatt
    36121 gccaaatgaa atgttgccat gttcaccttg tttaaaatca aggtttgtaa tgtttccttg
    36181 tgtcacgata ataggcgtaa tatcactctt tgcatgattg cggatgtagt ctaaatcaaa
    36241 gttgattaat aaatcacctt gtttaacttc ttgaccttcc tcaacatgta aagtaaagcc
    36301 ttctccgttt aatttaacag tgtctaaacc gatgtggatt aatagttcta aaccactatc
    36361 tgatacaaga ccaattgcat gttttgttgg gaaaatcatt tgtactttac cgttgaatgg
    36421 tgcacgaact tcaccttgtg aaggtttgat agcgataccg tcacccatca ttttttcgct
    36481 gaacacttga tcaggcactt ctgataatgg tgttacttca ccagttaatg gtgcatgcac
    36541 gatatggctc aattcgcttg ttgcagattt atcttctgca acaacaacag tttcgtcttt
    36601 atcgtcttcc atagtagtag gattttctac tacttgacca ttcataatct gttgcatttc
    36661 atgtttgatt tggtcagatt ttggaccaaa aattgcttgc atattattac cgacttctaa
    36721 tacaccggat gcacctaaat ttttcaaacc aggaacatca actttagatt tatcgttgac
    36781 ttcaacacgt agacgtgtga tacaagcgtc taaatgttta atgtttgctt tgccacccat
    36841 agcttctaat actgcatatg gtaattcagt tgctgaagca gtagccgctt gtgattgttt
    36901 atcttcacga cctggtgttt tgtattttaa ttttacaatt aagaatcgga atacgaagta
    36961 gtaaataact gcgtatacaa gacctacagg aataactaac caccattgtg tcttattagg
    37021 tagtataccg agtaagaagt agtcgatgaa accacctgag aatgtataac ctagatgaag
    37081 atctaataag tacaatgtta agaatgataa accatcaagt actgcgtgaa taaagaataa
    37141 taatggtgct acaaataaga atgagaattc taaaggttct gtaataccag ttaagaatga
    37201 tgttaaagca gcagaaccca ttaaacctgc tactactttc ttgttttcag gtttagctgt
    37261 gtgataaatt gctaaagctg ctgcaggtaa accgaacatc ataacaggga attcaccttg
    37321 catgaattta ccagctgtca aatgtgcgcc ttcacgaatt tgttcgataa agatacgttg
    37381 gtcaccgtga ataatttcac cagctgcatt tttccatgaa ccaaactcga accagaacgg
    37441 tgcgtggaaa atgtgatgta gaccgaatgg aattaataaa cgcttgatga aaccaaataa
    37501 gaatacggca acaccagtat ttgagtctaa taatcctgta ctgaatgcat ttaatcctgt
    37561 ttgaatcgtt ggccaaatta atgccattgg gaatgctaaa ataaatgatg ttgtagccat
    37621 cataataggt acgaaacgct taccagcgaa gaaacctaaa taagatggta agttaatgtt
    37681 atagaacttg ttataacacc aagctgccag ggccccgatt ataataccgc cgaacacacc
    37741 tgtttgtaat gttgggatac ctaaaatgct agcgtaacca ctcgctggat caccaatgtt
    37801 cttaggtgtt acttgtaaaa agtcacccat tgttttgttc atgattatgt aaccgacgaa
    37861 tgctgcgata gctgctacac catcaccgcc agctaatccg attgcgacac ctaatgcgaa
    37921 aatcataggc aagttatcaa aaatgatacc accagcaccc gtcattaatt tagcgacagt
    37981 ttgtacgcca ccattttgta taaacggcaa gtagtgttgt aatgattcac cttgcatagc
    38041 tgtaccgata gctaataaca gaccagctgc tggtaaaatc gcaacaggta acattagcgc
    38101 tttaccaata cgttgcaatt gaccgaaaag tttcttcctc acttgtccaa cctccaaagt
    38161 tgtatttatt atttaagatt caataaaaaa ggcacgagca aatagactgt gttggtaacc
    38221 atcacagctt aatttaactc atgcctaatc ttacttagta acacgttggt gtatgtaatt
    38281 aatgtaaaag aagcaattga ctaagtagta tataaaacgg atacgttagt ttatctagct
    38341 taccatcaca tcttattgaa tcttatttgt ttgcgactcc tattttagca tggtgaagta
    38401 tgcgttttca atacaattat taaattcaat ttcaaatctc ttaattgttc acagattatt
    38461 attaaaacat aatataagat aatcttgaat aaaatgtata aaagttgttg taacaattca
    38521 gcaatagcag ctattatcag cacttaaaag aaaggataat ctttgatggt gtaataatta
    38581 acttgtgaca aaaatatgta agattattac aatgaacagg ctttaactga ttaatattgt
    38641 aatgtgtttt ctcttatagt aaaatgtaag cgattacaca actcaactat attttcctaa
    38701 taattatatt gttaaggagg gctactttga caggcttttc agtgtattta ggacaacctt
    38761 tagatgaagc gtatattaag cgaatgatta aacaaggtta ccaaatgatt tttacatctg
    38821 tacaaatacc agaagaagat gacgagacaa aatatcatta tttcacaaaa ctactcaatt
    38881 tattaaaaca tgaacaagtg acttacctca tagatgctaa tccatctatt ttaacaccat
    38941 ctttttatga gcatcttcgg caatatgatg cacaatttat gattcgtatc gatcatagta
    39001 catcaattga ggcaatcgaa gcgataatgg cacagggttt aaagtgctgt ttgaatgcaa
    39061 gtattatttc ccgggaattg ttaacaagct tacatcaaca attgaatgat tttacattac
    39121 tttcattttg tcataactat tatccaagac cagatacggg attatctgtt gacttggtca
    39181 ataagaaaaa tgaactcatt tatcaattta atccaaaggc acaaatatat ggttttattg
    39241 tagggagtgg tttgcgaggt cctttgcata aaggcttgcc aacaattgaa gcaacgagac
    39301 atagtcatcc tgtcgttgca gctaaattac tacaagaaac tggtgtatct gaagtgttag
    39361 ttggagactc attgattgaa atgaggcagg caaaacaact tatagatttt tgcaagcata
    39421 ggcatttcac gttatgtatt gaagaagtgt ttgatacgac ggtgacttac cttttcgata
    39481 tgtgtcataa agtacgcccg gataatccgg aaaatgtcat tcgttcggaa acgtcaagac
    39541 aaatatgtcc acattcgatt caaccacagt ttacgacgca acgacgcatt ggttcagtaa
    39601 ccgttgataa tttgaataac ggacgttatc aaggtgaaat gcaaattgtg agacaaacgc
    39661 ttagtgcaca tgacaatgtg aatgttgttg cacaaattat taaagaagac ttaccactgt
    39721 taagttgtat cgagccgaat gatacatttg attttcaaaa aactagggag tgtaagaagt
    39781 gatggaaaat agtacgaccg aagcgcgtaa tgaagcgacg atgcatcttg atgaaatgac
    39841 tgtggaagag gctttaatta cgatgaataa agaagatcag caagtcccgt tagcagttcg
    39901 aaaggcaata ccacaattga caaaagtaat taaaaaaaca attgcacagt ataaaaaggg
    39961 tggacgattg atctatatcg gtgcaggtac aagtggaaga ttgggtgtct tagatgcagc
    40021 ggagtgtgta cctacattca atactgaccc tcatgaaatt ataggtatta ttgctggtgg
    40081 acaacatgct atgacgatgg ctgtagaagg tgcggaagat cacaaaaaat tagcggaaga
    40141 agatttgaaa aatatagatt taacatcaaa agatgtcgtt ataggaattg ccgcgagtgg
    40201 caaaacgcca tatgttatag gcggtttaac atttgctaac acaatcggtg ctacaacagt
    40261 atctatttca tgcaatgaac atgcagttat aagtgaaatt gcgcagtatc cagtagaagt
    40321 taaagttggt ccagaagtat taactggttc aacgcgttta aagtctggta cagcacaaaa
    40381 attaatttta aatatgattt caaccatcac aatggttggt gtcggaaaag tttacgataa
    40441 cctcatgatt gatgttaaag caaccaatca aaaactgatc gaccgttcag tgcgtattat
    40501 tcaagaaata tgtgctatca catatgatga agcaatggcg ttatatcagg tatctgagca
    40561 tgatgtgaaa gttgcgacag ttatgggtat gtgtggcatt tctaaggaag aagcaacaag
    40621 acggttacta aacaatggtg acattgttaa acgagcaatc agagatagac aaccttagga
    40681 gggatttaaa tgaccaaaga acaacaactt gcagaacgaa ttattgctgc agtaggtggt
    40741 atggataata tagatagtgt catgaactgt atgacacgtg tgcgtattaa agtattagat
    40801 gaaaataaag tagatgatca agaactaagg catattgatg gtgtcatggg tgttatacac
    40861 gatgaacgca ttcaagttgt ggttggacct ggtacagtca ataaagtggc taatcatatg
    40921 gcggaattaa gtggtgttaa actaggtgac ccaataccac accatcacaa tgatagtgaa
    40981 aaaatggact ataaatcata tgcagctgat aaagcaaagg cgaataaaga agcacataaa
    41041 gcaaaacaaa agaatggtaa gtcgaataaa gtattgaaat caattgccaa tatctttata
    41101 ccgttgattc ctgcatttat tggagctgga ttaattggtg gtattgcagc agtactgagt
    41161 aacttaatgg tggcaggtta tatttcaggt gcttggatta cgcaacttat aacagtattt
    41221 aatgtcatta aagacggtat gttagcatac ttagctattt tcactggtat taatgcggct
    41281 aaagaatttg gtgcgacacc aggacttggt ggcgtgattg gtggtacaac gttattaacg
    41341 ggtattgctg gtaaaaatat tttaatgaat gtcttcactg gagaaccatt gcaacctgga
    41401 caaggtggaa ttattggcgt tatttttgcc gtttggattt taagtattgt cgaaaagaga
    41461 ttgcataaaa ttgtgccaaa tgcgattgat attattgtaa cgccgactat tgcattgttg
    41521 atcgtaggac tattaactat ctttatcttt atgccattag caggttttgt ttcagacagt
    41581 ttagtttcag tagttaacgg aattattagt attggtggcg tatttagtgg atttatcatt
    41641 ggtgcaagct tcctaccgtt agttatgtta gggcttcatc atatttttac gccaattcat
    41701 atagaaatga ttaaccaatc tggtgctact tacttattgc caatagcagc gatggctggt
    41761 gctggacaag taggtgccgc attagcactt tgggtaagat gtaaacgcaa cacaacatta
    41821 cgtaatactt taaaaggtgc attgccagtt ggtttcctag gtattggaga accattaatc
    41881 tatggtgtga ctttgccatt aggtcgacct ttcttaactg cttgtattgg cggtggtatt
    41941 ggtggtgctg taataggtgg aattggacat attggtgcca aagcaatagg cccaagtggt
    42001 gtgtcactat taccattaat ctcagataat atgtatttag gttatattgc agggttactt
    42061 gctgcgtatg ctggtggatt cgtttgtaca tatttatttg gaacgacaaa ggcgatgcga
    42121 cagacagatt tgttgggtga ttaatgatga caaatatttt atatcgcatt gataagcagt
    42181 tgagtgattt tacgaagaca gaaaagataa tcgctgatta cattttaaag aatccacata
    42241 aaatcattga tatgactgtg aatgatttgg cagatgttac gaatgttagt acagcatcaa
    42301 ttgttagatt tagtcggaaa atgacacatc aaggttttca agagctaaag attgcgatat
    42361 ctcgatactt acccgaagat attgcaacca atccacattt ggaattgatt gaaaatgaac
    42421 ctgtagaaac tttgaaaaat aaaatgattg ctagagcaac gaatacgatg cgatttgtag
    42481 ctactaatat tatggatgcg caaattgatg caatttgtga tgtgttgaaa aatgccagga
    42541 caatattttt atttggattt ggcgcatcga gtttgactat tggtgatctt tttcaaaagt
    42601 tatctcgtat tggcttaaat gtcaggttat tacatgaaac gcatttactt gtgtcaacat
    42661 ttgcgacgca tgatgataga gattgcatga tttttgtgac gaatcaaggt agtcatagtg
    42721 aattgcagtc aattgcacag gtggccacac attacagtat tcccatcata actatatcta
    42781 gtacagctaa taatccagtg gctcaaattg cagactatgc attgatttat ggcagaactg
    42841 atgaaaatga aatgcgtatg gcggctacaa cgtcactatt tgcacagtta ttcacggtag
    42901 atatattgta ctatcgattt gtagcattaa attatcatgc gattctagat tgtataaccc
    42961 aatcgaaaat ggcacttgat aattacagga agcatcttgc gacgatagat tttaaacatt
    43021 agatattgtg cgcgttctaa tataaagtta gttgcaacgt aaacaattga agtttttgat
    43081 gtgattcagt tgcttgatga aaaattgttg ctacaagttt gtaaaactgg catagaggct
    43141 attttgaaaa tgataaaggc gtgacaaatt aaagcaaagt tcagatttga gctttgcttt
    43201 tttcatagat ggaaattaaa tgctgttatt aagggggaat taatttggaa cttaacgaga
    43261 tgattaattt tgtaaaagaa aacaataaga taatggttac tttatcgcta acaattttca
    43321 cgtctgtaat atttcctgag ttaaaaagtt ggtattcaaa aatcattaat actgaaaata
    43381 aatatagaat attcgatagg gcaattatta tgattggcac agtgttacca atgtattttc
    43441 ttttaggatt tttgaattat aaaatatata ttaaactgct agaaagacct catagtgaat
    43501 ttattaatac aatatggttt tcat
//
LOCUS       DAHHOJ010000007        42337 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.007, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000007 DAHHOJ010000000
VERSION     DAHHOJ010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 42337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42337
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.007"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            1..765
                     /locus_tag="NBH32_000300"
     CDS             1..765
                     /locus_tag="NBH32_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1513938.1"
                     /translation="MILKFDHIIHYIDQLDRFSFPGDVIKLHSGGYHHKYGTFNKLGY
                     INENYIELLDVENNEKLKKMAKTIEGGVAFATQIVQEKYEQGFKNICLRTNDIEAVKN
                     KLQSEQVEVVGPIQMERDTHKDGKVKWQLLYIMNQDDDEIKPPFFIQWEESDSMRTKK
                     LQKYIQKQFSIETVIVKSKNRSQTVSNWLKWFDMDIVEENDHYTDLILKNDDIYFRIE
                     DGKVSKYHSVIIKDAQATSPYSIFVRGAIYRFEPLV"
     gene            complement(757..1008)
                     /locus_tag="NBH32_000301"
     CDS             complement(757..1008)
                     /locus_tag="NBH32_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513939.1"
                     /translation="MRTIILSLFIIMNIVAIIMTLSQPLTVNYFSLRVILIFFTFILS
                     IFFILIKSSRLNNILTILSIVLAIIHMGILAHSTYVYLY"
     gene            1397..2662
                     /gene="femX"
                     /locus_tag="NBH32_000302"
                     /gene_synonym="fmhB"
     CDS             1397..2662
                     /gene="femX"
                     /locus_tag="NBH32_000302"
                     /gene_synonym="fmhB"
                     /EC_number="2.3.2.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II:glycine glycyltransferase"
                     /protein_id="HCD1513940.1"
                     /translation="MEKMHITNQEHDAFVKSHPNGDLLQLTKWAETKKLTGWYARRIA
                     VGRDGEVQGVAQLLFKKVPKLPYTLCYISRGFVVDYSNKEALNALLDSAKEIAKAEKA
                     YAIKIDPDVEVDKGTDALQNLKALGFKHKGFKEGLSKDYIQPRMTMITPIDKNDDELL
                     NSFERRNRSKVRLALKRGTTVERSDREGLKTFAELMKITGERDGFLTRDISYFENIYD
                     ALHEDGDAELFLVKLDPKENIAKVNQELNELHAEIAKWQQKMETSEKQAKKAQNMIND
                     AQNKIAKNEDLKRDLEALEKEHPEGIYLSGALLMFAGSKSYYLYGASSNEFRDFLPNH
                     HMQYTMMKYAREHGATTYDFGGTDNDPDKDSEHYGLWAFKKVWGTYLSEKIGEFDYVL
                     NQPLYQLIEQVKPRLTKAKIKISRKLKRK"
     gene            2779..5946
                     /locus_tag="NBH32_000303"
     CDS             2779..5946
                     /locus_tag="NBH32_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HCD1513941.1"
                     /translation="MIKKLLQFSLGNKFAIFLMVVLVVLGGVYASAKLKLELLPNVQN
                     PVISVTTTMPGATPQSTQDEISSKIDNQVRSLAYVKNVKTQSIQNASIVTVEYENNTD
                     MDKAEEQLKKEIDKIKFKDEVGQPELRRNSMDAFPVLAYSFSNKENDLKKVTKVLNEQ
                     LIPKLQTVDGVQNAQLNGQTNREITLKFKQNELEKYGLTADDVENYLKTATRTTPLGL
                     FQFGDKDKSIVVDGQYQSVDAFKNINIPLTLAGGQGQSQSQSDNKQNSAMSDVNQASP
                     QQNSKASSSNNISGVPTAKLGDLADITVGDVRTSISKTNGKDAVNLQITKAQDANTVQ
                     VAKDVQRKIDTFVDENKDFNVTKTMDTAKPVEKSLYTMVEKASLGTIVAIIVILLFLR
                     NIRTTAISIISIPLSLLMALIALKLSDVSLNILTLGALTVAIGRVIDDSIVVVENIYR
                     RLTDSEEQLKGENLIISATTEVFKPIMSSTLVTIIVFLPLVFVSGSVGEMFRPFALAI
                     AFSLLASLLVSITLVPALAATLFKKGVKRRNKQHQEGLGVVSTTYKKVLHWSLNHKWI
                     VIILSTLILVVTIVFGGPRLGTSFISAGDDKFLAITYTPKPGETEQAVLNHAKDVEKY
                     LKQKKHVKTIQYSVGGSSPVDPTGSTNSMAIMVEYDNDTPNFDVEADKVIKHADGFKH
                     PGEWKNQDLGTGAGNKSVEVTVKGLSMDAIKSTVKDIEQKMKQVKGLANVKSDLSQTY
                     DQYEIKVDQNKAAENGISASQLAMHLNENLPEKTVTTVKENGKTVDVKVKQNKQTDWS
                     EDKLNNITLKKPTGGTIKLGDIATLVKTTTPSKLTQEQGDYATTVSAEVTNKDVGGTT
                     RQVMSKINNLDKPNNVKVNIGGASDDINNAMTQLAFAMLAAIIIVYLILVITFKGGLA
                     PFTILFSLPFTVIGVIIALLITGETISVPSLIGMLMLIGIVVTNAIVLIDRVINNEQQ
                     GMEMKEALIEAGGTRIRPILMTAIATIGALVPLLFGQDSSILISKGLAATVIGGLISS
                     TLLTLVVVPVIYEILFTLKKRFTKR"
     gene            complement(6133..6450)
                     /gene="mspA"
                     /locus_tag="NBH32_000304"
     CDS             complement(6133..6450)
                     /gene="mspA"
                     /locus_tag="NBH32_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane stabilizing protein MspA"
                     /protein_id="HCD1513942.1"
                     /translation="MQFYLILLAILYLIVSFISIFKMEVVFTRILRIIMGVLLLFVLA
                     LTTMSFPKENWWVFIVLLLLVGNVEVTGFKMLKKDLKGVNILNLMSLFIFVIYFILTI
                     VLF"
     gene            complement(6695..6859)
                     /locus_tag="NBH32_000305"
     CDS             complement(6695..6859)
                     /locus_tag="NBH32_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513943.1"
                     /translation="MSLENQLAELKYDYVRLQGDLEKRESLDLDTSALVRQLKDIENE
                     IRNVRAQMQD"
     gene            6992..7900
                     /locus_tag="NBH32_000306"
     CDS             6992..7900
                     /locus_tag="NBH32_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002487080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HCD1513944.1"
                     /translation="MTEQFTMIILLIALGYFLKRINFIKATDSQVIATLVLNVTLPSL
                     VIVNLNSAELKLSFSILPILMIIYGIVAKMIAVWFFRKYDNHMRGSVGMMTGAMNIGL
                     FAYPLIEVIWPKTGLIYFGMADIGGAFVMFGITYFVGSYFSEGSDQFDFKYLGKKLLQ
                     SVPLVTYIVMFILNISHIHLPHVAIDFFSILSHANMPLSMILLGVMLNFTLERKYIPA
                     TIKYLCLHYGLALVGGLLVHVLLPVSDDMIKTTLLVTWMFPVGVAIISYGIQFKYRTL
                     SFIGMTTNLTIIISIIILYVYQAVFV"
     gene            8307..8438
                     /locus_tag="NBH32_000307"
     CDS             8307..8438
                     /locus_tag="NBH32_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513945.1"
                     /translation="MYGAYLINEYHQDFKSIANFKVLKRMINVMMCYCVLFLEPRIS"
     gene            complement(8555..9418)
                     /locus_tag="NBH32_000308"
     CDS             complement(8555..9418)
                     /locus_tag="NBH32_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein GlcU"
                     /protein_id="HCD1513946.1"
                     /translation="MQFLDFLIALLPALFWGSVVLINVFVGGGPYNQIRGTTLGALIV
                     GLGLLITGFAKFNNPTVIIVGLISGALWAFGQANQLKSISLIGVSNTMPVSTGMQLVG
                     TTLFSVIFLGEWSSMTQIIFGLIAMILLVTGVALTSLKAKNERQSDNPEFKKAMGILI
                     VSTVGYVGFVVLGDIFGVGGTDALFFQSVGMAIGGFILSMNHKTSLKSTALNLLPGVI
                     WGIGNLFMFYSQPKVGVATSFSLSQLLVIVSTLGGIFILGERKDRRQMTGIWAGIIII
                     VIAAIILGNLK"
     gene            complement(9599..10486)
                     /locus_tag="NBH32_000309"
     CDS             complement(9599..10486)
                     /locus_tag="NBH32_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1513947.1"
                     /translation="MSIVQLYDITQIKSFIEHSNYESASYLYKLPQQYNEIDVLITDA
                     IESPGVFSIKENDSIKAIILSFAYDKNKFKVIGPFVADNYVLSVDTFEKLFKAMTSNQ
                     PDDAVFNFSFEEGIQQYKPLMKVIQASYNFTDYYIEARTRLEEDMHQPNIIPYHKGFY
                     RAFSKLHTTTFKYQAQSPQDIIDSLDDHHHLFLFVSEGLLKGYLYLEIDSQQSIAEIK
                     YFSSHVDYRLKGIAFELLAYALQYAFDNFDIRKVYFKIRNKNNKLIERFNGLGFHINY
                     EYIKFKFESRNVKDQTIPE"
     gene            10552..10647
                     /locus_tag="NBH32_000310"
     CDS             10552..10647
                     /locus_tag="NBH32_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513948.1"
                     /translation="MILTCYNTFNTSFAIIEFKSKISGIDEARSK"
     gene            10673..12808
                     /locus_tag="NBH32_000311"
     CDS             10673..12808
                     /locus_tag="NBH32_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCD1513949.1"
                     /translation="MKSLILAEKPSVARDIADALQINQKRNGYFENNQYIVTWALGHL
                     VTNATPEQYDKNLKEWRLEDLPIIPKYMKTVVIGKTSKQFKTVKALILDNKVKDIIIA
                     TDAGREGELVARLILDKVGNKKPIRRLWISSVTKKAIQQGFKNLKDGRQYNDLYYAAL
                     ARSEADWIVGINATRALTTKYDAQLSLGRVQTPTIQLVNTRQQEINQFKPQQYYTLSL
                     TVKGFDFQLESNQRYTNKETLEQMVNNLKNVDGKIKSVATKHKKSYPQSLYNLTDLQQ
                     DMYRRYKIGPKETLNTLQSLYERHKVVTYPRTDSNYLTTDMVDTMKERIQATMATTYK
                     DQARPLMSKTFSSKMSIFNNQKVSDHHAIIPTEVRPVMSDLSNRELKLYDMIVERFLE
                     ALMPPHEYDAITVTLEVAGHTFVLKENVTTVLGFKSIRQGESITEMQQPFSEGDEVKI
                     SKTNIREHETTPPEYFNEGSLLKAMENPQNFIQLKDKKYAQTLKQTGGIGTVATRADI
                     IDKLFNMNAIESRDGKIKVTSKGKQILELAPEELTSPLLTAQWEEKLLLIERGKYQAK
                     TFINEMKDFTKDVVNGIKNSDRKYKHDNLTTTECPTCGKFMIKVKTKNGQMLVCQDPS
                     CKTKKNVQRKTNARCPNCKKKLTLFGKGKEAVYRCVCGHSETQAHMDQRMKSKSSGKV
                     SRKEMKKYMNKNEGLDNNPFKDALKNLNL"
     gene            12921..14255
                     /locus_tag="NBH32_000312"
     CDS             12921..14255
                     /locus_tag="NBH32_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000800694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HCD1513950.1"
                     /translation="MKNYFQFDKYGTNFKREILGGITTFLSMAYILAVNPQVLSLAGV
                     KGVSEDMKMDQGAIFVATALAAFVGSLFMGLIAKYPIALAPGMGLNAFFAFTVVLTMG
                     IPWQVGLTGVLFSGIFFAILTVTGFREVIINAIPYQMKMAVSAGIGLFITFVGLQSSG
                     IIVKNESTLVTLGHLTDPPVLLAIFGIVITVILYAIKLPGSIFIGMIITAIVGMFTGL
                     IQMPSGIVGKIPSIEPTFGAAFEAFKDPSQLLTIQFLIVILTFLFIDFFDTAGTLVAV
                     ATQAGIMKDNKLPRAGRALFSDSLATIVGSVFGTTTTTSYIESTSGVAVGARTGFASI
                     VTGCCFLLALFFSPLIAVVTSAVTTPALVVVGVLMAANFAEINWKSFEVAVPAFITII
                     MMPLSYSIATGIACGFIFYPITMLISKKHKEVHPIMYVLMVLFILYFIFVHG"
     gene            complement(14446..14835)
                     /locus_tag="NBH32_000313"
     CDS             complement(14446..14835)
                     /locus_tag="NBH32_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513951.1"
                     /translation="MKNGPKLSLALIGIFLILCEFFYGIPFLGATFILSFGWQPLLFN
                     ALLYLILTIILLVNRQNAIRPIAIIPIFGIVGSFLAIIPFLGILIHWILFFLMILFVL
                     VVLSAPTYIPNKNARVIYTQYKDGHRE"
     gene            15192..15500
                     /gene="rpsJ"
                     /locus_tag="NBH32_000314"
     CDS             15192..15500
                     /gene="rpsJ"
                     /locus_tag="NBH32_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001118669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCD1513952.1"
                     /translation="MAKQKIRIRLKAYDHRVIDQSAEKIVETAKRSGADVSGPIPLPT
                     EKSVYTIIRAVHKYKDSREQFEQRTHKRLIDIVNPTPKTVDALMGLNLPSGVDIEIKL
                     "
     gene            15528..16190
                     /gene="rplC"
                     /locus_tag="NBH32_000315"
     CDS             15528..16190
                     /gene="rplC"
                     /locus_tag="NBH32_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000160215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCD1513953.1"
                     /translation="MTKGILGRKIGMTQVFGENGELIPVTVVEAKENVVLQKKTVEVD
                     GYNAIQVGFEDKKAYKKDAKSNKYANKPAEGHAKKADAAPKRFIREFRNVDVDAYEVG
                     QEVSVDTFVAGDVIDVTGVSKGKGFQGAIKRHGQSRGPMSHGSHFHRAPGSVGMASDA
                     SRVFKGQKMPGRMGGNTVTVQNLEVVQVDTENKVILVKGNVPGPKKGLVEIRTSIKKG
                     NK"
     gene            16217..16840
                     /gene="rplD"
                     /locus_tag="NBH32_000316"
     CDS             16217..16840
                     /gene="rplD"
                     /locus_tag="NBH32_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCD1513954.1"
                     /translation="MANYDVLKLDGTKSGSIELSDAVFGIEPNNSVLFEAINLQRASL
                     RQGTHAVKNRSAVSGGGRKPWKQKGTGRARQGTIRAPQWRGGGIVFGPTPRSYAYKMP
                     KKMRRLALRSALSFKAQENGLTVVDAFNFEAPKTKEFKNVLSTLEQPKKVLVVTENED
                     VNVELSARNIPGVQVTTAQGLNVLDITNADSLVITEAAAKKVEEVLG"
     gene            16840..17115
                     /gene="rplW"
                     /locus_tag="NBH32_000317"
     CDS             16840..17115
                     /gene="rplW"
                     /locus_tag="NBH32_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002506966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCD1513955.1"
                     /translation="MEARDILKRPVITEKSSEAMAEDKYTFDVDTRVNKTQVKMAVEE
                     IFNVKVASVNIMNYKPKKKRMGRYQGYTNKRRKAIVTLKEGSIDLFN"
     gene            17148..17981
                     /gene="rplB"
                     /locus_tag="NBH32_000318"
     CDS             17148..17981
                     /gene="rplB"
                     /locus_tag="NBH32_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCD1513956.1"
                     /translation="MAIKKYKPITNGRRNMTSLDFAEITKTTPEKSLLKPLPKKAGRN
                     NQGKLTVRHHGGGHKRQYRVIDFKRNKDGINAKVDSIQYDPNRSANIALVVYADGEKR
                     YIIAPKGLEVGQIVESGAEADIKVGNALPLQNIPVGTVVHNIELKPGKGGQIARSAGA
                     SAQVLGKEGKYVLIRLRSGEVRMILSTCRATIGQVGNLQHELVNVGKAGRSRWKGIRP
                     TVRGSVMNPNDHPHGGGEGRAPIGRPSPMSPWGKPTLGKKTRRGKKSSDKLIVRGRKK
                     K"
     gene            18048..18326
                     /gene="rpsS"
                     /locus_tag="NBH32_000319"
     CDS             18048..18326
                     /gene="rpsS"
                     /locus_tag="NBH32_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCD1513957.1"
                     /translation="MARSIKKGPFVDEHLMKKVEAQEGSEKKQVIKTWSRRSTIFPNF
                     IGHTFAVYDGRKHVPVYVTEDMVGHKLGEFAPTRTFKGHVADDKKTRR"
     gene            18355..18708
                     /gene="rplV"
                     /locus_tag="NBH32_000320"
     CDS             18355..18708
                     /gene="rplV"
                     /locus_tag="NBH32_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000387528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCD1513958.1"
                     /translation="MEAKAVARTIRIAPRKVRLVLDLIRGKNVAEAIAILKLTNKASS
                     PVIEKVLMSALANAEHNYDMNTDELVVKEAYANEGPTLKRFRPRAQGRASAINKRTSH
                     ITIVVSDGKEEAKEA"
     gene            18732..19385
                     /gene="rpsC"
                     /locus_tag="NBH32_000321"
     CDS             18732..19385
                     /gene="rpsC"
                     /locus_tag="NBH32_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCD1513959.1"
                     /translation="MGQKINPIGLRVGIIRDWEAKWYAEKDFASLLHEDLKIRKFIDN
                     ELKEASVSHVEIERAANRINIAIHTGKPGMVIGKGGSEIEKLRNKLNALTDKKVHINV
                     IEIKKVDLDARLVAENIARQLENRASFRRVQKQAITRAMKLGAKGIKTQVSGRLGGAD
                     IARAEQYSEGTVPLHTLRADIDYAHAEADTTYGKLGVKVWIYRGEVLPTKNTSGGGK"
     gene            19388..19822
                     /gene="rplP"
                     /locus_tag="NBH32_000322"
     CDS             19388..19822
                     /gene="rplP"
                     /locus_tag="NBH32_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCD1513960.1"
                     /translation="MLLPKRVKYRRQHRPKTTGRSKGGNYVTFGEFGLQATTTSWITS
                     RQIESARIAMTRYMKRGGKVWIKIFPHTPYTKKPLEVRMGAGKGAVEGWIAVVKPGRI
                     LFEVAGVSEEVAREALRLASHKLPVKTKFVKREELGGETNES"
     gene            19812..20021
                     /gene="rpmC"
                     /locus_tag="NBH32_000323"
     CDS             19812..20021
                     /gene="rpmC"
                     /locus_tag="NBH32_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCD1513961.1"
                     /translation="MKAKEIRDLTTSEIEEQIKSSKEELFNLRFQLATGQLEETARIR
                     TVRKTIARLKTVAREREIEQSKANQ"
     gene            20045..20308
                     /gene="rpsQ"
                     /locus_tag="NBH32_000324"
     CDS             20045..20308
                     /gene="rpsQ"
                     /locus_tag="NBH32_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011983253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCD1513962.1"
                     /translation="MSERNDRKVYVGKVVSDKMDKTITVLVETYKTHKLYGKRVKYSK
                     KYKTHDENNSAKLGDIVKIQETRPLSATKRFRLVEIVEESVII"
     gene            20340..20708
                     /gene="rplN"
                     /locus_tag="NBH32_000325"
     CDS             20340..20708
                     /gene="rplN"
                     /locus_tag="NBH32_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCD1513963.1"
                     /translation="MIQQETRLKVADNSGAREVLTIKVLGGSGRKTANIGDVIVCTVK
                     NATPGGVVKKGDVVKAVIVRTKSGVRRNDGSYIKFDENACVIIRDDKGPRGTRIFGPV
                     ARELREGNFMKIVSLAPEVL"
     gene            20744..21061
                     /gene="rplX"
                     /locus_tag="NBH32_000326"
     CDS             20744..21061
                     /gene="rplX"
                     /locus_tag="NBH32_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCD1513964.1"
                     /translation="MHIKKGDNVKVIAGKDKGKEGKVIATLPKKDRVVVEGVNIMKKH
                     QKPTQLNPEGGILETEAAIHVSNVQLLDPKTNEPTRVGYKFVDGKKVRIAKKSGEEIK
                     SNN"
     gene            21088..21627
                     /gene="rplE"
                     /locus_tag="NBH32_000327"
     CDS             21088..21627
                     /gene="rplE"
                     /locus_tag="NBH32_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCD1513965.1"
                     /translation="MNRLKEKFNTEVTENLMKKFNYSSVMEVPKIDKIVVNMGVGDAV
                     QNSKVLDNAVEELELITGQKPLVTKAKKSIATFRLREGMPIGAKVTLRGERMYEFLDK
                     LISVSLPRVRDFQGVSKKAFDGRGNYTLGVKEQLIFPEIDYDKVSKVRGMDIVIVTTA
                     NTDEEARELLANFGMPFRK"
     gene            21650..21835
                     /locus_tag="NBH32_000328"
     CDS             21650..21835
                     /locus_tag="NBH32_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HCD1513966.1"
                     /translation="MAKTSMVAKQQKKQKYAVREYTRCERCGRPHSVYRKFKLCRICF
                     RELAYKGQIPGVRKASW"
     gene            21867..22265
                     /gene="rpsH"
                     /locus_tag="NBH32_000329"
     CDS             21867..22265
                     /gene="rpsH"
                     /locus_tag="NBH32_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000178880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCD1513967.1"
                     /translation="MTMTDPIADMLTRVRNANMVRHEKLELPASNIKKEIAEILKSEG
                     FIKNVEYVEDDKQGVLRLFLKYGQNDERVITGLKRISKPGLRVYAKASEMPKVLNGLG
                     IALVSTSEGVITDKEARKRNVGGEIIAYVW"
     gene            22290..22826
                     /gene="rplF"
                     /locus_tag="NBH32_000330"
     CDS             22290..22826
                     /gene="rplF"
                     /locus_tag="NBH32_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCD1513968.1"
                     /translation="MSRVGKKIIDIPSDVTVTFDGNHVTVKGPKGELSRTLNERMTFK
                     QEENTIEVVRPSDSKEDRTNHGTTRALLNNMVQGVSQGYVKVLELVGVGYRAQMQGKD
                     LILNVGYSHPVEIKAEENITFSVEKNTVVKVEGISKEQVGALASNIRSVRPPEPYKGK
                     GIRYQGEYVRRKEGKTGK"
     gene            22857..23216
                     /gene="rplR"
                     /locus_tag="NBH32_000331"
     CDS             22857..23216
                     /gene="rplR"
                     /locus_tag="NBH32_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCD1513969.1"
                     /translation="MISKIDKNKVRLKRHARVRTNLSGTAEKPRLNVYRSNKHIYAQI
                     IDDNKGVTLAQASSKDSDIATTATKVELATKVGEAIAKKAADKGIKEIVFDRGGYLYH
                     GRVKALAEAARESGLEF"
     gene            23237..23737
                     /gene="rpsE"
                     /locus_tag="NBH32_000332"
     CDS             23237..23737
                     /gene="rpsE"
                     /locus_tag="NBH32_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCD1513970.1"
                     /translation="MARREEETKEFEERVVTINRVAKVVKGGRRFRFTALVVVGDKNG
                     RVGFGTGKAQEVPEAIKKAVEAAKKDLVVVPRVEGTTPHTITGRYGSGSVFMKPAAPG
                     TGVIAGGPVRAVLELAGITDILSKSLGSNTPINMVRATIDGLQNLKNAEDVAKLRGKT
                     VEELYN"
     gene            23754..23933
                     /gene="rpmD"
                     /locus_tag="NBH32_000333"
     CDS             23754..23933
                     /gene="rpmD"
                     /locus_tag="NBH32_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCD1513971.1"
                     /translation="MAKLQITLTRSVIGRPETQRKTVEALGLKKTNSSVVVEDNPAIR
                     GQINKVKHLVTVEEK"
     gene            23950..24390
                     /gene="rplO"
                     /locus_tag="NBH32_000334"
     CDS             23950..24390
                     /gene="rplO"
                     /locus_tag="NBH32_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCD1513972.1"
                     /translation="MKLHELKPAEGSRKERNRVGRGVATGNGKTSGRGHKGQKARSGG
                     GVRPGFEGGQLPLFRRLPKRGFTNINRKEYAIVNLDQLNKFEDGTEVTPALLVESGVV
                     KNEKSGIKILGNGSLDKKLTVKAHKFSASAAEAIDAKGGAHEVI"
     gene            24390..25682
                     /gene="secY"
                     /locus_tag="NBH32_000335"
     CDS             24390..25682
                     /gene="secY"
                     /locus_tag="NBH32_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCD1513973.1"
                     /translation="MIQTLVNFFRTKEVRNKIFFTLAMLVIFKIGTYIPAPGVNPAAF
                     DNPQGSQGATELLNTFGGGALKRFSIFAMGIVPYITASIVMQLLQMDIVPKFSEWAKQ
                     GEVGRRKLNNVTRYLAISLAFIQSIGMAFQFNNYLKGALIINQSIMSYLLIALVLTAG
                     TAFLIWLGDQITQFGVGNGISIIIFAGILSTLPASLIQFGQTAFVGQEDTSLAWLKVL
                     GLLVSLILLTVGAIYVLEAVRKIPIQYAKKQTAQRLGSQATYLPLKVNSAGVIPVIFA
                     MAFFLLPRTLTLFYPDKEWAQNIANAANPSSNVGMVVYIVLIILFTYFYAFVQVNPEK
                     MADNLKKQGSYVPGIRPGEQTKKYITKVLYRLTFVGSIFLAVISILPILATKFMGLPQ
                     SIQIGGTSLLIVIGVAIETMKSLEAQVSQKEYKGFGGR"
     gene            25699..26346
                     /locus_tag="NBH32_000336"
     CDS             25699..26346
                     /locus_tag="NBH32_000336"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCD1513974.1"
                     /translation="MNIILMGLPGAGKGTQASEIVKKFPIPHISTGDMFRKAIKEETE
                     LGKEAKSYMDRGELVPDEVTVGIVKERISEDDAKKGFLLDGFPRTIEQAEALNNIMSE
                     LDRNIDAVINIEVPEEELMNRLTGRRICESCGTTYHLVFNPPKVEGICDIDGGKLYQR
                     EDDNPETVANRLSVNIKQSKPILDFYDQKGVLKNIDGSKDISDVTKDVIDILDHL"
     gene            26539..26757
                     /gene="infA"
                     /locus_tag="NBH32_000337"
     CDS             26539..26757
                     /gene="infA"
                     /locus_tag="NBH32_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCD1513975.1"
                     /translation="MAKQDVIELEGTVLDTLPNAMFKVELENGHEILAHVSGKIRMNY
                     IRILPGDKVTVEMSPYDLTRGRITYRYK"
     gene            26789..26902
                     /gene="rpmJ"
                     /locus_tag="NBH32_000338"
     CDS             26789..26902
                     /gene="rpmJ"
                     /locus_tag="NBH32_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCD1513976.1"
                     /translation="MKVRPSVKPICEKCKVIKRKGKVMVICENPKHKQRQG"
     gene            26925..27290
                     /gene="rpsM"
                     /locus_tag="NBH32_000339"
     CDS             26925..27290
                     /gene="rpsM"
                     /locus_tag="NBH32_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCD1513977.1"
                     /translation="MARIAGVDIPREKRVVISLTYIYGIGTSTAQKILEEANVSADTR
                     VKDLTDDELGRIREVVDGYKVEGDLRRETNLNIKRLMEISSYRGIRHRRGLPVRGQKT
                     KNNARTRKGPVKTVANKKK"
     gene            27314..27703
                     /gene="rpsK"
                     /locus_tag="NBH32_000340"
     CDS             27314..27703
                     /gene="rpsK"
                     /locus_tag="NBH32_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCD1513978.1"
                     /translation="MARKQVSRKRRVKKNIENGVAHIRSTFNNTIVTITDEFGNALSW
                     SSAGALGFKGSKKSTPFAAQMASETASKSAMEHGLKTVEVTVKGPGPGRESAIRALQS
                     AGLEVTAIRDVTPVPHNGCRPPKRRRV"
     gene            27778..28722
                     /locus_tag="NBH32_000341"
     CDS             27778..28722
                     /locus_tag="NBH32_000341"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCD1513979.1"
                     /translation="MIEIEKPRIETIEISEDAKFGKFVVEPLERGYGTTLGNSLRRIL
                     LSSLPGAAVKYIEIEGVLHEFSAVDNVVEDVSTIIMNIKQLALKIYSEEDKTLEIDVR
                     DEGEVTASDITHDSDVEILNPELKIATVSKGGHLKIRLVANKGRGYALAEQNNTSDLP
                     IGVIPVDSLYSPVERVNYTVENTRVGQSSDFDKLTLDVWTNGSITPQESVSLAAKIMT
                     EHLNIFVGLTDEAQNAEIMIEKEEDQKEKVLEMSIEELDLSVRSYNCLKRAGINSVQE
                     LADKSEADMMKVRNLGRKSLEEVKYKLEDLGLGLRKED"
     gene            28739..29107
                     /gene="rplQ"
                     /locus_tag="NBH32_000342"
     CDS             28739..29107
                     /gene="rplQ"
                     /locus_tag="NBH32_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002450507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCD1513980.1"
                     /translation="MGYRKLGRTSDQRKAMLRDLATSLIISERIETTEARAKEVRSVV
                     EKLITLGKKGDLASRRNAAKTLRNVEILNEDETTQTALQKLFGEIAERYTERQGGYTR
                     ILKQGPRRGDGAESVIIELV"
     gene            29645..30454
                     /locus_tag="NBH32_000343"
     CDS             29645..30454
                     /locus_tag="NBH32_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD1513981.1"
                     /translation="MEDKNSVIVFKNVSFQYQSDASFTLKDVSFNIPKGQWTSIVGHN
                     GSGKSTIAKLMIGIEKVKSGEIFYNNQAITDDNFEKLRKDIGIVFQNPDNQFVGSIVK
                     YDVAFGLENHAVPHDEMHRRVSEALKQVDMLERADYEPNALSGGQKQRVAIASVLALN
                     PSVIILDEATSMLDPDARQNLLDLVRKVKSEHNITIISITHDLSEAMEADHVIVMNKG
                     TVYKEGTAIEIFDHAEELTTIGLDLPFPIKINQMLGYQTSFLTYEGLVDQL"
     gene            30451..31311
                     /locus_tag="NBH32_000344"
     CDS             30451..31311
                     /locus_tag="NBH32_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HCD1513982.1"
                     /translation="MTIRFDNVSYTYQKGTPYQHQAIHDVNTEFEQGKYYAIVGQTGS
                     GKSTLIQNINALLKPTTGTVTVDDITITHKTKDKYIRPVRKRIGMVFQFPESQLFEDT
                     VEREMIFGPKNFKMNLDEAKNYAHRLLMDLGFSRDVMSQSPFQMSGGQMRKIAIVSIL
                     AMNPDIIVVDEPTAGLDPQSKRQVMRLLKSLQTDENKAIILISHDMNEVARYADEVIV
                     MKEGSIVSQTSPKELFKDKEKLADWHIGLPEIVQLQYDFEQKHQTKLKDIALTEEAFV
                     SLYKEWQHEK"
     gene            31301..32107
                     /locus_tag="NBH32_000345"
     CDS             31301..32107
                     /locus_tag="NBH32_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1513983.1"
                     /translation="MKNKLIIGRYLPINSFVHHLDPRAKLMFVFLFIILIFFCHSPLT
                     YLWVFALILFFMRLAKIQLWFLIKGLTPIFFFLIFTLMMHIFLTKGGYVLVEWHGITI
                     ETNGILEGLYISLRLIGIVMIATIMTLSTSPIDLTDAFERLLAPLKMFKLPVHQLSMI
                     MSIALRFIPTLMDELDKIILAQKSRGSEISSGNIATRIKSFIPLLVPLFISAFQRAEE
                     LAVAMEVRGYDANVQRTSYRQLKWQLRDTLSLIMIIPFAIILFVLKYSGV"
     gene            32112..32915
                     /gene="truA"
                     /locus_tag="NBH32_000346"
     CDS             32112..32915
                     /gene="truA"
                     /locus_tag="NBH32_000346"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCD1513984.1"
                     /translation="MRILVEIAYQGNNFLGFQIQQNGRTVQQQFEKLLQRMHKRHVRI
                     HPSSRTDRGVHAIQQYFHFDTELNIPMSQWQYAMNRTLPDDIYVNNVVTVDDDFHCRY
                     DCVGKRYRYKVYQAQHRDPFQSGLKTFIPETLDLDKMNRAAQQFIGTHDFTGFCSQKT
                     EVESKVRTLYQSEIVKTDDGFDYIVTGSGFLYNMVRVLVAFLIEVGKGRHEVSDVPKL
                     LESKNRKNVPFTAPAEGLYLEKIYLDENELLKDFGNDIKIHRKKSLQND"
     gene            33155..33592
                     /gene="rplM"
                     /locus_tag="NBH32_000347"
     CDS             33155..33592
                     /gene="rplM"
                     /locus_tag="NBH32_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019468667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCD1513985.1"
                     /translation="MRQTFMANESNIERKWYVIDAEGQTLGRLSSEVASILRGKNKAT
                     YTPHVDTGDYVIVINASKIEFTGNKETDKVYYRHSNHPGGIKSITAGELRRTNPERLI
                     ENSIKGMLPSTRLGEKQGKKLFVYGGAEHPHAAQQPENYELRG"
     gene            33606..34004
                     /gene="rpsI"
                     /locus_tag="NBH32_000348"
     CDS             33606..34004
                     /gene="rpsI"
                     /locus_tag="NBH32_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCD1513986.1"
                     /translation="MTLAQVEYRGTGRRKNSVARVRLVPGEGNITVNNRDVREYLPFE
                     SLILDLNQPFDVTETKGNYDVLVNVHGGGFTGQAQAIRHGIARALLEADPEYRGSLKR
                     AGLLTRDPRMKERKKPGLKAARRSPQFSKR"
     gene            34593..34772
                     /locus_tag="NBH32_000349"
     CDS             34593..34772
                     /locus_tag="NBH32_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513987.1"
                     /translation="MTEIVEFLSDGKLASRLEIQETMGLQKSYTSELIAELKKLEIIG
                     SEGRGLGTRYYLIRD"
     gene            35422..35604
                     /locus_tag="NBH32_000350"
     CDS             35422..35604
                     /locus_tag="NBH32_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513988.1"
                     /translation="MKDSIFWKKAFIPVYFIVAMLVFLLFRFYIKTDNFSMYLMIIFL
                     ICLGTASIIYNYKNNR"
     gene            complement(35586..35866)
                     /locus_tag="NBH32_000351"
                     /pseudo
     CDS             complement(35586..35866)
                     /locus_tag="NBH32_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500937.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            36099..37763
                     /gene="alsS"
                     /locus_tag="NBH32_000352"
     CDS             36099..37763
                     /gene="alsS"
                     /locus_tag="NBH32_000352"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HCD1513989.1"
                     /translation="MTDKKYTAADMVIDTLKNNGVEYVFGIPGAKIDYLFNALIDDGP
                     ELIVTRHEQNAAMMAQGIGRLTGKPGVVLVTSGPGVSNLTTGLLTATSEGDPVLALGG
                     QVKRNDLLRLTHQSIDNAALLKYSSKYSEEVQDPESLSEVMTNAIRIATSGKNGASFI
                     SIPQDVISSPAESKAISLCQKPNLGVPSEQDINDVIEAIKNASFPVLLAGMRSSSADE
                     TNAIRKLVERTNLPVVETFQGAGLISRELENHFFGRVGLFRNQVGDELLRKSDLVVTI
                     GYDPIEYEASNWNKELDTQIINIDEVQAEITNYMQPKKELIGNIAKTIEMISDKVDEP
                     FINQQHLDELEQLRAHIDQETGIKATHEEGILHPVEIIESMQKVLTDETTVTVDVGSH
                     YIWMARNFRSYNPRHLLFSNGMQTLGVALPWAISAALVRPNTQVVSVAGDGGFLFSSQ
                     DLETAVRKNLNIIQLIWNDGKYNMVEFQEEMKYKRSSGVDFGPVDFVKYAESFGAKGL
                     RVTNQEELEAAIKEGYETDGPVLIDIPVNYKDNIKLSTNMLPDVFN"
     gene            37800..38504
                     /gene="budA"
                     /locus_tag="NBH32_000353"
     CDS             37800..38504
                     /gene="budA"
                     /locus_tag="NBH32_000353"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD1513990.1"
                     /translation="MASVLYQHGTLGTLMAGLLKGTASINELLQHGDLGIATLTGSNG
                     EVIFLDGKAYHANEHKEFVELKGDELTPYATVTKFVADTSYETKDKSSEAVFAEIKEK
                     MLSENLFSAVKISGLFKKMHVRMMPAQEPPYTRLIDSARRQPEQTETYVKGSVVGFFT
                     PELFHGIGSAGFHVHFANDDRNFGGHVLDFEVEDVKVEIQNIETFEQHFPIHDEDFTN
                     ANIDYKDISDEIREAE"
     gene            complement(38883..39308)
                     /locus_tag="NBH32_000354"
     CDS             complement(38883..39308)
                     /locus_tag="NBH32_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1513991.1"
                     /translation="MKLKSFVTATLALGLLSTVGAALPSHEASADSNNGYKEMTMDGY
                     HTVPYTISVDGITALHRTYFVFPENKKVLYQEIDSKVKNELASQRGVTTEKINNAQTA
                     TYTLTLNDGNKKVVNLKKNDDAKNSIDPSTIKQIQIVVK"
     gene            39603..40418
                     /locus_tag="NBH32_000355"
     CDS             39603..40418
                     /locus_tag="NBH32_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIB family hydrolase"
                     /protein_id="HCD1513992.1"
                     /translation="MSKRLLLFDFDETYFKHNTNEEDLSHLREMEKLLEKLTNNNEVI
                     TAVLTGSTFQSVMDKMDQVNMTFKPLHIFSDLSSKMFTWNNGEYVESETYKKKVLSEP
                     FLFEDIEDILRHISAQYNVEFIPQRAFEGNETHYNFYFHSTGNHNNDRRILEALVRYA
                     NDQNYTARFSRSNPLAGDPENAYDIDFTPSNAGKLYATQFLMKKYNIPVKSILGFGDS
                     GNDEAYLSYLEHAYLMSNSRDEALKQKFRLTKYPYYQGITLHVKEFVEGKYDY"
     gene            complement(40651..42168)
                     /locus_tag="NBH32_000356"
     CDS             complement(40651..42168)
                     /locus_tag="NBH32_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1513993.1"
                     /translation="MTTYMLMYSIPEKAGGITSVMLNRSKLLAEKGYSCALLTLDDKN
                     YDPLRAKLTNSGRLHKQVKIVNLYEELKMLYDVENTEIENKNMQTFEKLNLLNQEGFY
                     IQKDEYEEKKYARYFKDNHYIMYKKWINGQLSHIDYFQNRKRFKRHVFQNKILRKEIT
                     FNDKNKMSEVKYLSNDGFCYLSYWYGDNENIVNIFYFDKNSKEVLNFKNNKAFHSYWL
                     DKNLTSNDVLILDGIGTYHKVENMQNNDIKKIFTIHTNHFMSPYSYGAEIKPEFRNML
                     LNLKKLDTLVVLTKEQKDDIIKQFGDYNNIKVIPNAISFEENLTQNIREKNSIIVLQR
                     FVAMKNITHIISAINIVRKKVKDVKLHIYGTGTQKENYTKLIKKLKLQDNVFIHDYAF
                     DIRALYTKTSLSVLTSDYEGLPMSLLEAMSYGVPVISYPINYGPKSIIQNNINGIITK
                     KKDNINELAKKIIHVLKKETLISQFSENARTTIKTNFSYEAVSKKWIELIDEKSQ"
BASE COUNT        15159 a         6395 c         7882 g        12901 t
ORIGIN      
        1 atgatattga aatttgatca catcattcat tatatagatc agttagatcg gtttagtttt
       61 ccaggagatg ttataaaatt acattcaggt gggtatcatc ataaatatgg aacattcaat
      121 aaattaggtt atatcaatga aaattatatt gagctactag atgtagaaaa taatgaaaag
      181 ttgaaaaaga tggcaaaaac gatagaaggt ggagtcgctt ttgctactca aattgttcaa
      241 gagaagtatg agcaaggctt taaaaatatt tgtttgcgta caaatgatat agaggcagtt
      301 aaaaataaac tacaaagtga gcaggttgaa gtagtagggc cgattcaaat ggaaagagat
      361 acacataaag atggtaaggt aaagtggcaa ttgctttata taatgaatca ggatgatgat
      421 gaaattaagc caccattttt tattcaatgg gaagaaagtg attccatgcg tactaaaaaa
      481 ttgcaaaaat atattcaaaa acaattttca attgaaactg ttattgtgaa aagtaaaaac
      541 cgatcacaaa cagtatcgaa ttggttgaaa tggtttgata tggacattgt agaagagaat
      601 gaccattaca cagatttgat tttaaaaaat gatgatattt attttagaat tgaagatggt
      661 aaagtttcaa aatatcattc ggttatcata aaagacgcac aagcaacttc accatattca
      721 atttttgtca gaggtgctat ttatcgcttt gaaccattag tataaatata cgtaagtgct
      781 atgagcgaga atgcccatat gaataatggc aagcacaatg gaaagaatcg ttaatatatt
      841 atttaatcgt gatgacttaa ttaaaatgaa aaagattgat aatataaatg tgaaaaagat
      901 aagtataacc cgtaaactaa agtaattcac ggtgagaggt tgactcaatg tcataatgat
      961 tgcaacgatg ttcataatta taaatagact taaaataatt gttctcatat caaacacctc
     1021 attgttagat tattgacatt ataacagggg tatttgtata tgaacattaa tgtggttgct
     1081 tgaggaaaaa tttattcatt gaagtcaagt tggttcattt tagaaatgaa tatcgtgtta
     1141 gatgatgaaa gtatattgaa gtataggtaa ctagttgaaa agtattaatt gtacgataac
     1201 attaaattta acacgaaaca tagatataaa atgatttaca attaaaatgg gtaaatttga
     1261 acttgctaaa ctattaattg gagcatggac atttcaaaaa taagagttca aatcttacac
     1321 aagctctgaa tcgacactat aagatacaaa ctgtataatt aaaggtattg ttaaatagaa
     1381 ggagatatca taaatcatgg aaaagatgca tatcactaat caggaacatg acgcatttgt
     1441 taaatctcac ccaaatggag atttattaca attaacgaaa tgggcagaaa caaagaaatt
     1501 aactggatgg tacgcgcgaa gaatcgctgt aggtcgtgac ggtgaagttc agggtgttgc
     1561 gcaattactt tttaaaaaag tacctaaatt accttatacg ctatgttata tttcacgtgg
     1621 ttttgttgtt gattatagta ataaagaggc gttaaatgca ttgttagaca gtgcaaaaga
     1681 aattgctaaa gctgagaaag cgtatgcaat taaaatcgat cctgatgttg aagttgataa
     1741 aggtacagat gctttgcaaa atttgaaagc gcttggtttt aaacataaag gatttaaaga
     1801 aggtttatca aaagactaca tccaaccacg tatgactatg attacaccaa ttgataaaaa
     1861 tgatgatgag ttattaaata gttttgaacg ccgaaatcgt tcaaaagtgc gcttggcttt
     1921 aaagcgaggt acgacagtgg aacgatctga tagagaaggt ttaaaaacat ttgctgaatt
     1981 aatgaaaatc actggggaac gcgatggctt cttaacgcgt gatattagtt actttgaaaa
     2041 tatttatgat gcgttgcatg aagatggaga tgctgaacta tttttagtaa agttggaccc
     2101 aaaagaaaat atagcgaaag taaatcaaga attgaatgaa cttcatgccg aaatagctaa
     2161 atggcagcag aagatggaaa catctgaaaa gcaagctaaa aaagcgcaaa atatgattaa
     2221 tgatgcgcaa aataaaattg ctaaaaatga agatttaaaa cgagacctag aagctttaga
     2281 aaaggaacat cctgaaggta tttatctttc tggtgcacta ttaatgtttg ctggctcaaa
     2341 atcatattac ttatatggtg cgtcttctaa tgaatttaga gattttttac caaatcacca
     2401 tatgcagtat acgatgatga agtatgcacg tgaacatggt gcaacaactt acgatttcgg
     2461 tggtacagat aatgatccag ataaagactc agaacattat ggcttatggg catttaaaaa
     2521 agtgtggggt acatacttaa gtgaaaagat tggtgaattt gattatgtat tgaatcagcc
     2581 attgtaccaa ttaattgagc aagttaaacc tcgtttaaca aaagctaaaa ttaaaatatc
     2641 tcgtaaatta aaacgaaaat agattaaaga ctgaaatctg aacgctcata agactgtcat
     2701 ttgcgttcag atttttttac acaatataga atggttgagt aaaatatttt tgaatatagt
     2761 gaaagagggg gaagtactgt gataaaaaag ctattacaat tttctttggg gaataagttt
     2821 gctatctttt taatggttgt tttagttgtc ttgggcggtg tatacgcgag tgctaaattg
     2881 aaattagaat tactaccaaa tgtacaaaat ccagttattt cagttacaac aacaatgccg
     2941 ggtgcaacgc cacaaagtac ccaagatgaa ataagtagta aaattgacaa ccaagtaaga
     3001 tcgttggcat atgtgaaaaa tgttaaaacg caatccatac aaaatgcttc aattgtaaca
     3061 gttgaatatg aaaataatac agatatggat aaagcagaag aacagcttaa aaaagaaatc
     3121 gataaaatta aatttaaaga tgaagttggt caaccagaat taagacgtaa ttcgatggac
     3181 gcttttccgg ttttagcata ttcattttca aataaagaga atgacttgaa aaaagtaacg
     3241 aaagtactga atgaacaatt aataccaaaa ttacaaacgg tagatggtgt gcaaaatgcg
     3301 caattaaacg ggcagacgaa ccgtgaaatc acccttaaat ttaagcaaaa tgaacttgaa
     3361 aaatatgggt tgactgctga tgatgtagaa aactatctaa aaacggcaac aagaacaaca
     3421 ccacttggat tgttccaatt tggtgataaa gataaatcaa ttgttgttga tggtcaatat
     3481 caatctgttg atgcttttaa aaacataaat attccattaa cgttggcagg aggacaaggg
     3541 caatctcaat ctcaaagtga caataaacaa aattcagcca tgtcagacgt taatcaggca
     3601 tcaccacagc aaaattcaaa agcgtcatca tcaaataata taagtggtgt gccgacagcg
     3661 aaactaggag atttagctga tattacagtt ggtgatgtgc gtacttctat ttctaaaacg
     3721 aatggcaagg atgcggttaa tttacaaata actaaagctc aagatgccaa tacagttcaa
     3781 gtagccaaag atgtacaacg taaaattgat acattcgttg atgaaaataa agattttaat
     3841 gtcacaaaaa caatggatac tgcaaagcct gttgagaaat cactttatac gatggttgaa
     3901 aaagcatcat taggtacaat cgtggcaatt atagttattt tgctgttttt aagaaacatt
     3961 cgtacgacgg caatttctat tatatccatt ccgttatcac ttcttatggc gcttattgct
     4021 ctgaaattga gtgatgtttc attgaatata ctaacgttag gtgcattaac agtagcgatt
     4081 ggacgtgtga tagacgattc gattgtagtt gttgaaaata tttatcgacg cttaacagat
     4141 tcagaagaac aactaaaagg tgaaaattta attatcagtg cgacaactga agtatttaaa
     4201 ccaataatgt catcgacact agttactatt atcgtcttct taccacttgt gtttgtatca
     4261 ggttcagtag gcgaaatgtt tagacctttt gcattggcta ttgcatttag tttattagca
     4321 tcgttattag tgtcaattac actcgttcca gcgttggcag caacactatt taaaaaaggc
     4381 gttaaacgtc gtaataaaca acatcaagaa ggattaggtg ttgttagtac aacttataaa
     4441 aaagtattgc attggtcact aaatcataag tggattgtaa ttatattaag tacattaatt
     4501 ttggttgtaa ctattgtatt tggaggaccg agactaggca ctagctttat ttcagcaggt
     4561 gacgataaat ttttagctat tacttataca ccgaagcctg gtgaaacgga gcaagcagtg
     4621 ttgaatcatg cgaaagatgt tgaaaaatat ttaaaacaga aaaagcatgt aaaaacaatt
     4681 caatactcag ttggtggtag tagtccagta gatccaacgg gtagtacaaa tagtatggca
     4741 atcatggttg aatatgataa tgacacgcct aattttgatg tagaagcgga taaggttatt
     4801 aaacatgcag atggctttaa acatcctgga gagtggaaaa atcaagattt aggaacaggt
     4861 gcaggtaata aatctgtaga ggttactgtg aaaggcctat caatggatgc cataaaatca
     4921 actgtaaaag atattgaaca gaaaatgaaa caggttaaag gactagccaa tgtcaaatct
     4981 gatttatcgc aaacatatga tcagtatgaa attaaagttg atcaaaataa agcggcagaa
     5041 aatggtattt ctgcaagtca acttgcaatg cacttgaatg aaaacttacc agaaaaaaca
     5101 gttacgactg ttaaagaaaa tggtaaaact gttgatgtta aagtcaaaca aaataagcaa
     5161 acagactggt cagaagataa gttgaataat ataactttga aaaagccgac tggtggcacg
     5221 attaaattgg gagatatcgc tacgttagtt aaaacaacga caccaagtaa attgacgcaa
     5281 gaacaaggtg attatgcaac gacggtatct gctgaagtaa caaataaaga tgtgggtggc
     5341 acaacacgac aagtgatgtc taaaataaat aatttggaca aaccgaataa tgtaaaggtt
     5401 aatatcggtg gtgcatcaga tgatattaac aatgcaatga cacaattagc ctttgcaatg
     5461 ttagctgcga tcattatcgt atatttaatc ctagttatta catttaaagg tggcttagca
     5521 ccatttacaa ttctattctc tttaccattt acagttatcg gtgtaattat tgcactatta
     5581 atcacaggag aaacaatatc agtaccaagt ttaattggta tgctaatgtt aattggaatc
     5641 gtagtaacaa atgccattgt gttaatagac cgtgtcatta ataatgagca gcagggcatg
     5701 gagatgaaag aagcattaat cgaagcaggc ggtactagaa ttagaccgat attaatgacg
     5761 gcgattgcaa caattggtgc attagttcct ttgttatttg gtcaagatag ctcgattctt
     5821 atttcgaaag gattagctgc tacagttatt ggtggtttaa tttcatcaac tttattaaca
     5881 cttgtagttg taccagtaat atatgaaatt ctctttactt taaaaaaacg attcactaaa
     5941 cgatagataa tataaaagcc ataaaagcgg tcatggtatt gactactttt atggcttttt
     6001 aaaatttgaa attttagacc ttatttcacg tgcgatttaa tcgatgtgtt aataaaacaa
     6061 tactggcatt caacatgtaa tccgataata aaataagatt gcaattatgt tattgcatgt
     6121 tttgtttatt acttagaata atacgatggt taagatgaaa tatatgacaa agataaataa
     6181 tgacattaaa ttcaagatgt ttacgccttt tagatctttt ttaagcattt taaatcctgt
     6241 cacttcgaca ttaccgacta agagtaataa gacgataaat acccaccaat tctcttttgg
     6301 aaaactcatc gtcgttaatg ctaagacgaa taataacaac acacccataa taattctcaa
     6361 aatgcgagta aatacaactt ccattttaaa aatactaata aaactaacaa ttagataaag
     6421 tattgctagt aaaatcagat aaaattgcat atgataactt cctctccgtt tcaaagggtt
     6481 catgtagtac agcagcgtag aaaacataaa tagcagtgtt gggatagata tgcgcgtatg
     6541 ttattaatga gacactaaat cataacacga caacaccttg tgattctaga tgtaattgag
     6601 cgaaaaataa agcaattcat caatataacg tattcagagc aatgctagtc aaatttatgt
     6661 aactaaaagt atttattgcc tgaatgatag attattaatc ttgcatttga gcacgaacat
     6721 ttctaatttc attttcaata tctttaagtt gacgaacaag tgcggaagta tctaaatcca
     6781 aagattcccg tttttctaag tcgccttgaa gacgaacgta atcatattta agttcggcta
     6841 gttggttttc taaagacata ctataacact cctttgaaat ataattaagt gcatttaatg
     6901 tagattatag catgaaacag tagacttcta aaatatctgt ggagtatgtt aaagttataa
     6961 attatgaaga ttattgaaag aagggataac agtgacagaa caatttacta tgattatttt
     7021 attaattgcg cttggttact ttttgaagcg cattaatttt ataaaggcaa ctgatagcca
     7081 agtcattgct acattagttc ttaatgtcac actcccatct ttagttattg tgaatttaaa
     7141 cagtgcagaa ttaaaattgt cattctccat tttaccaatt ttaatgatta tctatggtat
     7201 tgtggcgaaa atgattgctg tctggttttt tagaaaatat gataatcaca tgcgtggatc
     7261 agtaggtatg atgactggtg ctatgaatat tggattgttt gcatatccac taattgaagt
     7321 gatatggcct aaaacggggc ttatttattt tggtatggct gatataggtg gtgcctttgt
     7381 gatgtttgga attacttact ttgtaggtag ctattttagt gaaggtagtg atcaattcga
     7441 ttttaaatat ttaggtaaga aattattaca atcagtgcca ctcgttacat acattgtaat
     7501 gtttatattg aatataagtc acatccattt accacatgtg gcaatcgatt tctttagcat
     7561 actttcccat gcaaatatgc cgttatcaat gattttgcta ggtgtcatgt tgaattttac
     7621 tctggaaaga aagtatatac ctgctactat taaatattta tgtttgcatt atggattagc
     7681 attagttggg ggattgttag tacatgtgtt gttaccggta tcagatgata tgattaagac
     7741 gacgttgttg gtcacttgga tgttcccagt tggtgtcgca attatttctt atggtattca
     7801 gtttaaatat cgaacactgt catttatagg tatgacgacc aatttgacaa tcattattag
     7861 tatcattatt ttgtacgtgt atcaggcagt gtttgtctag tttacacatt tgaaaataaa
     7921 atccatttta atagtcgaag aaaatgagag ccccctttac cacaacatgt aaatgtatat
     7981 tgtgaaaagg gggcttttat ttatgacata gttgttcaac aggctgggac ataaatcaat
     8041 attctatgct ctacgaagtt atattggcag tagttgactg aatgaaaatg cgcttgtaac
     8101 aagctttttt caattctagt caggggcccc aacaaagaga aattggattc ccaatttcta
     8161 cagacaatgc aagttggggt gtgggcccca acacagagaa tttcgaaaag aaattctaca
     8221 ggcaatgcga gttggggtgg gacgacgaaa tatattttgc gaaaatatca tttctgtccc
     8281 actccctatg cagctatcca cttcatatgt atggcgcata cttgataaat gaatatcatc
     8341 aagattttaa aagtatcgct aattttaaag tcttaaaacg catgattaat gtcatgatgt
     8401 gttattgtgt tttgttttta gagccacgta tttcctagga aatatagagg aacattcaac
     8461 gtaaaattct cgaaaattaa ttttaatcat tctataagtg aatggttatt ttcagaagcg
     8521 ataacatttt tactttacat gagtatttaa ctttctattt caaattacct agaattatag
     8581 cagctatcac gataataata atacctgccc aaatacccgt catctgacga cgatcttttc
     8641 tttctcctaa aatgaaaata ccgcctaagg ttgaaacgat aacaagtaac tgtgataatg
     8701 agaaacttgt agctacacca acttttggtt gagaatagaa catgaacaag ttaccaattc
     8761 cccaaatcac acctggcaat agattaagtg ctgttgattt aagtgatgtt ttatgattca
     8821 tggataggat aaagccacca attgccatac cgacagattg gaagaacaat gcatcagttc
     8881 caccaacacc aaagatgtca ccaagtacaa cgaaacctac atatccaact gtagatacaa
     8941 ttaaaatacc cattgctttt ttaaattcag gattatctga ttgacgttca tttttagctt
     9001 taagtgaagt aagtgctaca ccagtaacta ataatatcat ggcgattaaa ccaaagataa
     9061 tttgagtcat tgaagaccat tcacctaaaa agataacgct gaataatgtt gtaccaacta
     9121 attgcatacc tgtagaaact ggcatagtat ttgatacacc gattaaacta atagatttaa
     9181 gctgattcgc ttgtccaaac gcccataatg caccagaaat aagaccgaca ataattacag
     9241 taggattatt gaattttgcg aaaccagtaa taagtaaacc taatccaaca atgagtgcgc
     9301 ctagcgtcgt accacgaatt tggttgtaag gtccaccgcc gacgaacaca ttaataagaa
     9361 cgacacttcc ccagaataaa gcaggtaaaa gtgcgattaa gaaatcaaga aattgcaaga
     9421 ctactcactc tcctatacta tttatgcaag tgctcccctt aattgacact tgcatgttat
     9481 tgtaaagcat tctgccctaa tagtaaaaaa caactactta taattttcaa tatttacaaa
     9541 aagtagaaaa acccacaata catgctaatg atgtactgtt gggtatttgc ttggtatttt
     9601 attcagggat tgtttgatct tttacgttac gtgattcgaa tttgaattta atgtactcat
     9661 agttgatatg gaagcctaga ccattaaatc gttcgatgag tttattattt ttattacgaa
     9721 ttttaaaata aacttttcta atatcaaagt tatcaaaagc atattgcaat gcatacgcaa
     9781 gcaactcgaa agcgatacct ttcaaacggt aatctacatg tgaactgaag tatttaatct
     9841 cggcgattga ttgttgtgaa tcaatttcaa ggtataaata acctttaagt aaaccttcgc
     9901 taacaaataa aaacaaatga tgatggtcgt ctaaactatc aatgatatct tgtggtgact
     9961 gtgcctgata tttaaatgta gttgtgtgta atttgctgaa agcacgataa aaccctttgt
    10021 gataaggaat gatatttggt tggtgcatat cttcttctaa tcttgtacgg gcttctatgt
    10081 aatagtcagt gaagttataa cttgcttgaa taactttcat taatggtttg tattgttgaa
    10141 tgccttcttc aaaagaaaag ttaaagacgg catcgtcagg ttggttcgaa gtcattgctt
    10201 taaacagctt ttcaaacgta tcgacagata atacataatt gtcagctacg aaagggccta
    10261 tgactttgaa tttattttta tcgtatgcaa aagacaatat gattgctttg attgaatcgt
    10321 tttctttaat cgaaaataca ccaggtgatt caatcgcatc ggttattaat acatctattt
    10381 cattgtactg ttgaggaagt ttatataagt atgatgctga ttcataattc gaatgttcaa
    10441 tgaacgattt tatttgtgta atatcatata actgaactat gctcatgtca ttacctccgt
    10501 actttttgtt acttttatta tatagtattt caactgaaat gaaagttaat agtgatatta
    10561 acatgttaca atacatttaa cacatcattt gcaatcattg aatttaaatc aaagattagt
    10621 ggaatagatg aagcacgttc gaaataaaag aacgtatgag aaaggataat ttatgaaatc
    10681 tttaatatta gctgaaaaac catcagtcgc aagagatatt gctgatgctt tacaaataaa
    10741 tcagaagcgt aatggttact ttgaaaataa ccaatatatt gtcacgtggg cgttaggtca
    10801 tctagtgaca aatgcgacac ctgaacaata cgataaaaat ttaaaggaat ggcgattaga
    10861 agaccttcca attataccta aatatatgaa aactgttgtt attggtaaaa caagcaaaca
    10921 atttaaaaca gtaaaagcgt taattttaga taataaagtg aaagatatta ttattgcaac
    10981 agatgctgga cgagaaggtg aactagttgc aagattgatt ttggataaag ttggtaacaa
    11041 aaagccaatc cgtcgattat ggattagctc agttactaaa aaagctattc aacaaggttt
    11101 taaaaattta aaagacggtc gtcaatataa cgatttgtat tatgcagcgt tagcgagaag
    11161 tgaggcggat tggattgttg ggattaatgc aacgcgtgca ctaacaacaa agtatgatgc
    11221 acagctatcc ctgggacgtg ttcagacacc aacgattcaa ttagtaaata cacgacaaca
    11281 agagattaat cagttcaaac cacaacaata ctatacatta tcattaacgg taaaagggtt
    11341 tgattttcag ctagaatcaa atcagcgata taccaataaa gaaactttag aacagatggt
    11401 taataatttg aaaaatgtcg atggtaagat taaatctgtt gctactaaac ataagaagtc
    11461 gtatccgcaa tcactgtaca atttaacaga tttacaacaa gatatgtata gacgttataa
    11521 aattggacct aaagaaacat tgaatacact tcaaagttta tatgagagac ataaagtcgt
    11581 aacctatcca agaacagatt caaactattt aacaactgat atggtagata ctatgaaaga
    11641 acgtattcag gcgacgatgg caacaacata taaagaccaa gcacgcccat taatgtctaa
    11701 aacattttca tcaaaaatgt cgatatttaa taatcaaaaa gtatctgatc accatgcaat
    11761 tattcctaca gaagtgagac ctgtcatgtc agacttaagt aatagagaac taaagttata
    11821 cgatatgatt gtcgagcgtt ttttagaagc tttaatgcct ccgcacgagt atgacgcgat
    11881 aactgtaact ttagaggttg cagggcacac atttgttttg aaagagaatg taacaactgt
    11941 tttaggtttt aaatctatta gacaaggtga atctattaca gagatgcaac agcctttttc
    12001 agaaggcgat gaagtgaaga tttcaaaaac aaacattaga gaacatgaaa caacacctcc
    12061 agaatatttt aatgaaggtt cgttattaaa agcgatggag aaccctcaga actttattca
    12121 attgaaggat aaaaaatatg cgcaaacttt aaagcaaaca ggtggtatcg gcacagttgc
    12181 aacaagggcc gacattatcg ataaattatt taatatgaat gctattgaat caagagacgg
    12241 taaaattaaa gtaacgtcaa aaggtaaaca aatattagaa ttagcaccag aagaattaac
    12301 gtcgccactt ttaactgcac aatgggaaga aaaactactt ttaattgaac gtggtaaata
    12361 tcaggcgaaa acattcatta atgaaatgaa agattttacg aaagatgttg taaatgggat
    12421 taaaaatagt gatcgtaaat ataaacacga taatttaaca accactgaat gcccaacgtg
    12481 tggtaaattc atgattaaag ttaaaactaa aaatggtcag atgcttgtgt gccaagatcc
    12541 atcttgtaag acgaaaaaga atgtacagcg caaaacaaat gcaagatgtc caaactgtaa
    12601 aaagaaatta acgttgtttg gtaaagggaa agaagcggta tatcgttgtg tttgtggaca
    12661 ttctgaaacg caagcacata tggatcagcg tatgaagtct aaatcctctg gtaaagtatc
    12721 tcgtaaagaa atgaaaaagt atatgaataa aaatgaaggt ttagacaata atccgtttaa
    12781 agatgcatta aagaacttga atttatagat aaaatcgaac aaagttgaat cagaaaaacg
    12841 aaaagttcgc ttttggtatt gttttttatt aagaatgata ttaaactatt aaggtatttt
    12901 aaaaaaagga gcatccattc gtgaaaaact atttccagtt cgataaatat ggaacaaact
    12961 ttaaaagaga aatcttaggc ggtatcacaa ctttcttatc tatggcctat attttagcag
    13021 ttaacccgca agttttaagt ttagcaggtg ttaaaggcgt atcagaagat atgaaaatgg
    13081 accaaggtgc catttttgta gcgactgcat tagcagcatt tgtaggctcg ctattcatgg
    13141 gactaatagc taaatatcca attgcgttag caccaggtat gggattaaat gcattctttg
    13201 catttactgt tgtgttaacc atgggtattc cttggcaagt tggtttaaca ggcgttttat
    13261 tctcaggtat attctttgcg atattaaccg tcacagggtt tagggaagtt attattaatg
    13321 caattcctta tcaaatgaag atggctgttt cagcaggtat aggattattc attacatttg
    13381 taggtttaca aagttcgggt attatagtta aaaatgaatc tacattagta acattgggac
    13441 atttaactga tccaccagta cttttagcga tattcggtat cgttattaca gttattttat
    13501 atgcaattaa attgccgggt tctatcttta tcggtatgat catcacagca atcgttggta
    13561 tgttcactgg attgattcaa atgccatctg gtattgtagg taagattcca agtattgaac
    13621 caacgttcgg tgctgcattt gaagcgttta aagacccaag tcaattatta acgatacagt
    13681 tcttaattgt tattttaacg ttcttattta ttgatttctt tgatacagct ggtacattag
    13741 tagccgttgc tactcaagca ggtattatga aagataataa attaccaaga gctggtagag
    13801 cattgttctc agattcttta gctacgattg taggatcagt ttttggaaca actacaacaa
    13861 cttcttatat cgaatcaact tcaggtgttg ctgtaggtgc tagaacagga tttgctagta
    13921 tcgtaacagg ttgttgtttc ctattagcgc tgttctttag tccgttaatc gctgttgtaa
    13981 caagtgcagt tacgacacca gctttagtgg tggtaggtgt attaatggct gcaaactttg
    14041 ctgagataaa ctggaaaagt tttgaagttg cagtgccagc attcattaca atcattatga
    14101 tgcctttatc atactcaatt gcaactggta ttgcgtgtgg atttattttc tatccaatta
    14161 caatgcttat ctctaaaaaa cataaagaag tacatccgat aatgtatgta ttaatggtac
    14221 tattcattct gtacttcata tttgttcatg gttaaatcca tacacaaaaa acgactgcca
    14281 atgattggta gtcgtttttt ctttgaagta aggataaaat aagaagcgaa ataacataaa
    14341 gcaatattat gaatgtcttt aaatatccga tataaatata cttcttttaa aattgttgga
    14401 cgtgtttctt ctatattata tacctaatta aaaaggtttt aagaattatt ctctatgtcc
    14461 atctttgtat tgtgtgtaaa tgacacgagc atttttatta ggtatatagg ttggcgctga
    14521 taatacaact aggacaaata gaatcattag gaaaaataaa atccaatgaa tcaaaattcc
    14581 taagaagggg ataatagcta aaaaactacc tactatacca aatattggaa taatcgctat
    14641 aggtctaatc gcattttgtc tattcactaa taatataata gtaagaatta gataaagtaa
    14701 tgcattaaag agtaaaggct gccatccaaa acttaaaata aacgttgcac ctaaaaaagg
    14761 aatgccatag aaaaactcac aaagtattaa aaagatacct ataagtgcta aagataattt
    14821 tggtccattt ttcatattca ttcaccaccg ttcttatgac taattatata gaataatttt
    14881 caatgataat aataaaatga ttcaaggtac gaatttacaa taaagaagga aataaaaaat
    14941 atctcgaaaa tagttgaact gactaagtat ataaagtaaa atataaaagt atgtgttaga
    15001 cagatagaaa aattatgtga aaagccttga tttatcgatg tttttcgtgt aatataacta
    15061 acgttggact ttaagaaaag cgatgaagcg aaaggttact gacacacccg gccgctttgc
    15121 catggcgctg tgtaagatag ttttcgtgga gaagtctatc actaaatgta gacgaataag
    15181 gagggaaaat tatggcaaaa caaaaaatca gaatcagatt aaaagcttat gatcaccgcg
    15241 tgattgatca atcagcagag aagattgtag aaacagcgaa acgttctggt gcagatgttt
    15301 ctggaccaat tccgttacca actgagaaat cagtttacac aatcatccgt gccgtgcata
    15361 agtataaaga ttcacgtgaa caattcgaac aacgtacaca caaacgttta atcgatattg
    15421 taaacccaac accaaaaaca gttgacgctt taatgggctt aaacttacca tctggtgtag
    15481 acatcgaaat caaattataa tagacaattt taggaggtgg actttcgatg accaaaggaa
    15541 tcttaggaag aaaaattggg atgacacaag tattcggaga aaacggtgaa ttaatccctg
    15601 taacagtagt agaagctaaa gaaaatgttg tattacaaaa gaaaactgta gaagttgatg
    15661 gatacaacgc aatccaagtt ggatttgaag acaaaaaagc atacaaaaaa gatgcaaaat
    15721 ctaataaata tgctaataaa ccagctgaag gtcacgctaa aaaagctgac gcagcaccta
    15781 agcgcttcat tcgtgaattc cgcaatgtag acgtggatgc ttacgaagta ggtcaagaag
    15841 tctcagtaga tacttttgta gctggcgacg ttattgacgt aacaggcgta tcaaaaggta
    15901 aaggtttcca aggtgcaatt aaacgccacg gacaatctcg tggacctatg tcacatggtt
    15961 ctcatttcca cagagcacca ggttctgtag gtatggcttc agatgcttct agagtattta
    16021 aaggccaaaa aatgccagga cgtatgggtg gaaacacagt aactgttcaa aacttagaag
    16081 tagttcaagt tgacacagaa aacaaagtta tcttagtaaa aggtaacgta cctggaccta
    16141 aaaaaggttt agtagaaatc agaacttcaa ttaaaaaagg taataaataa taagaagtga
    16201 aaggaggaaa tgcataatgg ctaattatga tgttttaaaa ttagacggaa ctaaatcagg
    16261 ttcaatcgaa ttaagcgatg cagtattcgg tattgagcca aataatagcg ttttattcga
    16321 agctattaat ttacaacgtg cttcattacg tcaaggtacg catgctgtta agaatcgttc
    16381 agcagtaagc ggtggcggac gtaaaccatg gaagcaaaaa ggaacaggtc gtgctcgtca
    16441 aggtacaatc cgtgctccac aatggcgtgg cggtggtatc gtattcggac caactccaag
    16501 aagttatgca tacaaaatgc ctaagaaaat gcgtcgttta gctttacgct cagcattatc
    16561 tttcaaagct caagagaatg gcttaactgt agttgacgca tttaacttcg aagctccaaa
    16621 aactaaagaa ttcaaaaatg tattatctac attagaacaa cctaaaaaag tattagtagt
    16681 tactgaaaac gaagatgtaa atgttgaatt atcagcacgc aacatccctg gcgttcaagt
    16741 gacaactgct caaggtttaa atgttttaga tatcactaat gctgacagct tagtaattac
    16801 tgaagctgct gctaaaaaag ttgaggaggt gctcggataa tggaagcaag agatattctt
    16861 aagcgccccg taatcactga gaaatcttct gaagcaatgg ctgaagacaa atacactttc
    16921 gacgttgata ctcgtgttaa caaaacacaa gtaaaaatgg cagttgaaga aatcttcaac
    16981 gtaaaagttg caagtgttaa tatcatgaat tacaaaccta agaaaaaacg tatgggccgt
    17041 taccaaggct atacaaacaa aagaagaaaa gcgattgtaa ctcttaaaga aggatcaatc
    17101 gacttattta actaataaac caattaccat taaggaggta agcgacaatg gctattaaaa
    17161 agtataagcc aataacaaat ggtcgtcgta atatgacttc gttagatttc gcagaaatca
    17221 cgaaaactac acctgaaaag tcattattaa aaccgctacc gaaaaaagcg ggacgtaaca
    17281 accaaggtaa attgactgta agacaccatg gtggtggaca caaacgtcaa taccgtgtta
    17341 tcgatttcaa acgtaacaaa gatggtatca atgcaaaagt tgattctatt caatatgatc
    17401 caaaccgctc agcaaacatc gctttagttg tatatgcaga cggtgaaaaa cgatatatca
    17461 ttgctcctaa aggattagaa gtaggtcaaa tcgttgaaag tggtgctgaa gctgacatca
    17521 aagttggtaa cgcattacca ttacaaaaca ttccagttgg tacagtagta cacaacatcg
    17581 agcttaaacc tggtaaaggt ggacaaatcg ctcgttcagc tggtgcaagt gctcaagtac
    17641 ttggtaaaga aggtaagtac gtattaatca gattaagatc tggtgaagtt cgtatgatct
    17701 tatctacttg ccgtgctaca atcggtcaag ttggtaacct acaacacgaa ttagttaacg
    17761 ttggtaaagc cggacgttca agatggaaag gtattcgtcc aacagttcgt ggttctgtaa
    17821 tgaaccctaa cgatcaccca cacggtggtg gtgaaggtcg tgctcctatc ggtagaccat
    17881 ctccaatgtc accatggggt aaacctacgc ttggtaagaa aactcgtcgt ggtaaaaaat
    17941 catcagacaa acttatcgtt cgtggacgta agaaaaaata atataatcaa cttatttggg
    18001 tgtgcggctt aaagctgcac gcacataata agaagggagg cgcccaaatg gctcgtagta
    18061 ttaaaaaagg acctttcgtc gatgagcatt taatgaaaaa agttgaagct caagaaggaa
    18121 gcgaaaagaa acaagtaatc aaaacatggt cacgtcgttc tacaattttc cctaatttca
    18181 tcggacatac atttgcagta tacgacggac gtaaacacgt acctgtatat gtaactgaag
    18241 atatggtagg tcataaatta ggtgagtttg ctcctactcg tacattcaaa ggacacgttg
    18301 cagacgacaa gaaaacaaga agataatatc tattaagtag aggaggacat cctaatggaa
    18361 gcaaaagcgg ttgctagaac aataagaatc gcacctcgta aagtaagact agttcttgac
    18421 ttaatcagag gtaaaaatgt tgctgaagct attgcaattt taaaattaac aaacaaagct
    18481 tcatcaccag taattgaaaa agtattaatg tccgctttag ctaatgctga acataactat
    18541 gacatgaaca cagatgaatt agtagttaaa gaagcatatg ctaacgaagg accaacatta
    18601 aaacgtttcc gtccacgtgc gcaaggtcgt gcaagtgcga ttaacaaacg tacaagccac
    18661 attacaatcg tcgtaagtga cggtaaagaa gaagctaaag aagcttaatt aacttttaag
    18721 gagggaatac tgtgggtcaa aaaattaatc caatcggact tcgtgttggt attatccgtg
    18781 attgggaagc taaatggtat gctgaaaaag acttcgcttc acttttacac gaagatttaa
    18841 aaatccgtaa atttattgat aatgaattaa aagaagcatc agtttctcac gtagagattg
    18901 aacgtgctgc aaaccgtatc aacattgcaa ttcatactgg taaacctggt atggtaattg
    18961 gtaaaggcgg ttcagaaatc gaaaaattac gcaacaaatt aaatgcgtta actgataaaa
    19021 aagtacacat caacgtaatt gaaatcaaaa aagttgatct tgacgctcgt ttagtagctg
    19081 aaaacatcgc acgtcaatta gaaaaccgtg cttcattccg tcgtgtacaa aaacaagcaa
    19141 tcactagagc tatgaaactt ggtgctaaag gtatcaaaac tcaagtatct ggtcgtttag
    19201 gcggagctga catcgctcgt gctgaacaat attcagaagg aactgttcca cttcatacgt
    19261 tacgtgctga catcgattat gcacacgctg aagctgacac tacttacggt aaattaggcg
    19321 ttaaagtatg gatctatcgt ggagaagttc ttcctactaa gaacactagt ggaggaggaa
    19381 aataataatg ttactaccaa aacgtgtaaa atatcgtcgt caacatcgtc ctaaaacaac
    19441 tggtcgttct aaaggcggta actacgtaac atttggtgag tttggtttac aagctacaac
    19501 aacgtcttgg atcacatctc gtcaaatcga atctgctcgt atagcaatga cacgttacat
    19561 gaaacgtggc gggaaagttt ggattaaaat cttcccacat acaccatata ctaaaaaacc
    19621 tttagaagta cgtatgggtg ctggtaaagg tgcggttgaa ggctggatcg cagttgttaa
    19681 accaggtaga attttattcg aagttgctgg cgtttctgaa gaagttgcgc gtgaagcact
    19741 acgtttagca agtcacaaac ttccagtaaa aactaagttt gtaaaacgtg aggaattggg
    19801 tggtgaaaca aatgaaagct aaggaaatta gagacttaac cacttcagaa atcgaagaac
    19861 aaatcaaatc ttcaaaagaa gagcttttta acctacgctt tcagttagct acaggtcaat
    19921 tagaagaaac tgcacgtatt cgtacagtaa gaaaaacgat tgcacgtcta aaaactgttg
    19981 ctcgtgaaag agaaattgaa caaagtaagg ctaatcaata attaagtaag aggaggttac
    20041 aaaagtgagc gaaagaaacg atcgtaaagt ttatgtaggt aaagttgttt cagacaaaat
    20101 ggacaagact attacagtac ttgttgaaac ttacaaaaca cacaaattat acggtaaacg
    20161 agtaaaatac tctaaaaaat acaaaactca tgatgaaaac aattcagcta aattaggaga
    20221 cattgttaaa attcaagaaa ctcgtccttt atcagcaaca aaacgttttc gtttagtaga
    20281 gattgttgaa gagtcagtaa ttatttaata caagtttaga gataagggag gtttaactaa
    20341 tgatccaaca agaaacacgc ttgaaagtag cagacaactc tggtgctcgt gaagttctta
    20401 caatcaaagt attaggtgga tctggtcgta aaacagcaaa catcggcgat gttatcgtat
    20461 gtactgttaa aaatgcaaca ccaggtggcg ttgttaaaaa aggtgacgtt gtcaaagctg
    20521 taatcgtacg tactaagtca ggtgttcgtc gtaatgacgg ttcatacatc aaatttgatg
    20581 aaaatgcatg tgttatcatc cgtgatgaca aaggcccacg tggtactcgt atcttcggac
    20641 ctgttgctcg tgaattacgt gaaggtaact tcatgaaaat cgtatcatta gcaccagaag
    20701 tactttaatt aataacaacc aatacaaata aggaggtgcc cacatgcata tcaaaaaagg
    20761 tgacaacgtt aaagttatcg caggtaaaga caaaggtaaa gaaggtaaag taattgctac
    20821 tctacctaaa aaagaccgtg tcgttgtgga aggtgttaac attatgaaaa aacaccaaaa
    20881 accaactcaa ttaaatcctg aaggtggaat cttagaaaca gaggcagcaa tccatgtttc
    20941 taatgtacaa ttattggacc ctaaaacaaa cgaaccaact cgtgtaggtt acaaatttgt
    21001 tgatggtaaa aaagttcgta tcgctaaaaa atctggcgaa gaaattaaat ctaataatta
    21061 ataacaagtt gaaaggagga tcccactttg aaccgtttaa aagaaaagtt taacactgaa
    21121 gttactgaaa acttaatgaa aaaattcaat tatagttcag taatggaagt accaaaaata
    21181 gataaaatcg ttgtgaacat gggtgtaggt gacgcagtac aaaattctaa agtattagac
    21241 aatgctgttg aagaattaga attgatcact ggtcaaaaac cattagtaac taaagctaaa
    21301 aaatcaatcg cgactttccg tttacgtgaa ggtatgccaa tcggtgcgaa agtaacactt
    21361 cgcggtgaaa gaatgtatga attcttagac aaattaattt cagtatcatt accacgtgta
    21421 cgtgacttcc aaggtgtttc taaaaaagca tttgacggac gcggtaacta cactttaggt
    21481 gttaaagaac aattaatttt cccagaaatc gactatgata aagtaagtaa agttagagga
    21541 atggatattg ttatcgtaac gactgctaac actgatgaag aagctcgtga attgttagct
    21601 aacttcggta tgccattccg taaataatta tttaaaggag gctaattaag tggctaaaac
    21661 ttcaatggtt gctaagcaac aaaaaaaaca aaaatatgca gttcgtgaat acactcgttg
    21721 tgaacgttgt ggccgtccac attctgtata tcgtaaattt aaattatgcc gtatttgttt
    21781 ccgtgaatta gcttacaaag gccaaatccc tggcgttcgt aaagctagct ggtaataaaa
    21841 aagagtctga aaggaggcaa caatcaatga caatgacaga tccaatcgca gatatgctta
    21901 ctcgtgtaag aaacgcaaac atggtgcgtc acgagaagtt agaattacct gcatcaaata
    21961 ttaaaaaaga aattgctgaa atcttaaaga gtgaaggttt cattaaaaat gttgaatacg
    22021 tagaagatga taaacaaggt gtacttcgtt tattcttaaa atatggtcaa aacgatgagc
    22081 gtgttatcac aggattaaaa cgtatttcaa aaccaggttt acgtgtttat gcaaaagcta
    22141 gcgaaatgcc taaagtatta aatggtttag gtattgcatt agtatcaact tctgaaggtg
    22201 taatcactga caaagaagca agaaaacgta atgttggtgg agaaattatc gcatacgttt
    22261 ggtaataaaa aataaggagg tgccataaca tgagtcgtgt tggtaagaaa attattgaca
    22321 tccctagtga cgtaacagta acttttgatg gaaatcatgt aactgttaaa ggtcctaaag
    22381 gtgaattatc aagaacttta aatgaaagaa tgacattcaa acaagaagaa aacacaattg
    22441 aagttgtaag accatctgat tctaaagaag atagaacaaa ccatggtaca actcgtgctt
    22501 tattaaacaa tatggtacaa ggtgtttctc aaggatacgt aaaagtactt gaacttgttg
    22561 gtgtaggtta ccgtgctcaa atgcaaggta aagacttaat ccttaacgtt ggttattctc
    22621 acccagtaga aattaaagct gaagaaaaca ttactttctc agttgagaaa aacacagtcg
    22681 ttaaagttga aggtatttca aaagaacaag ttggagcatt agcatctaac atccgttcag
    22741 taagacctcc agaaccttac aaaggtaaag gtattcgtta ccaaggtgaa tacgttcgcc
    22801 gtaaagaagg taaaactggt aaataataga taaactctaa agaaaggagt attgaaatga
    22861 tcagtaaaat tgataaaaat aaagtgcgtt taaaaagaca tgctcgtgtt cgtactaact
    22921 tatcaggtac agctgaaaag ccacgtttaa acgtatatcg ttcaaacaag catatctacg
    22981 ctcaaattat tgatgataat aaaggcgtaa cattagctca agcttcttca aaagacagcg
    23041 acattgctac tacagcaact aaagttgaat tagcaactaa agtcggtgaa gcaattgcta
    23101 aaaaagctgc tgacaaaggc attaaagaaa tcgtatttga ccgtggagga tatttatatc
    23161 acggacgtgt taaagcatta gctgaagcag caagagaaag cggattagaa ttttaattta
    23221 aaggagggac aaatacatgg ctcgtagaga agaagagacg aaagaatttg aagaacgcgt
    23281 tgttacaatc aaccgtgtag caaaagttgt aaaaggtggt cgtcgtttcc gtttcactgc
    23341 attagttgta gttggagaca aaaatggtcg tgtaggtttc ggtactggta aagctcaaga
    23401 ggtaccagaa gcaatcaaaa aagctgttga agcagctaaa aaagatttag tagttgttcc
    23461 acgtgttgaa ggtacaactc cacacacaat tactggccgt tacggttcag gaagcgtatt
    23521 tatgaaaccg gctgcacctg gtacaggagt tatcgctggt ggtcctgttc gtgccgtact
    23581 tgaattagca ggtattactg atatcttaag taaatcatta ggatcaaaca caccaatcaa
    23641 catggttcgt gctacaatcg atggtttaca aaaccttaaa aatgctgaag atgttgcgaa
    23701 attacgtggc aaaacagtag aagaattata caattaagga gggaaaacta gttatggcta
    23761 aattacaaat taccctcact cgtagtgtta ttggtcgtcc tgaaacacaa cgtaaaactg
    23821 ttgaagcttt aggtcttaaa aagactaaca gttcagtagt tgttgaagat aaccctgcta
    23881 ttcgtgggca aatcaacaaa gttaagcact tagtaacagt agaagaaaaa taatcaagga
    23941 ggtgccgaaa tgaaattaca tgagttaaaa ccggcagaag gttcacgtaa agaacgcaat
    24001 cgtgttggac gtggtgttgc gacaggtaac ggtaaaacaa gtggtcgcgg acacaaaggt
    24061 caaaaagctc gttcaggcgg tggtgtaaga ccaggatttg aaggtggtca attaccatta
    24121 ttccgtcgtt taccaaaacg tggttttact aacataaatc gtaaagaata tgctattgtt
    24181 aacttagacc aacttaataa atttgaagat ggtactgaag taactccagc tttattagta
    24241 gaatctggtg ttgttaagaa tgaaaaatct ggtatcaaaa tactaggtaa tggttcactt
    24301 gataagaaat tgacagtgaa agctcataaa ttctcagctt cagcagcaga agctattgat
    24361 gctaaaggtg gagcacacga ggtgatctaa tgattcaaac ccttgtgaac ttctttagaa
    24421 caaaagaggt tcgtaacaag attttcttca cactagcaat gttagtaatt tttaaaatag
    24481 ggacttatat accagctcca ggagtaaatc ctgcagcttt tgataatccc caaggttctc
    24541 aaggtgccac tgagttatta aatacttttg gtggcggagc cttgaaacga ttttctattt
    24601 ttgcaatggg tattgtaccc tacatcactg catcaatcgt aatgcaatta ttacaaatgg
    24661 atattgtccc taaattctca gaatgggcaa aacaaggtga agtaggtaga agaaagttaa
    24721 ataacgttac tcgttattta gcaatttctt tagcatttat ccaatctata ggtatggcat
    24781 tccaatttaa taattatctc aaaggtgcgc tgattatcaa tcagtcaatt atgagttatt
    24841 tattaatagc actagttttg acagcaggaa ctgctttctt aatatggctt ggtgatcaaa
    24901 tcactcagtt cggtgttggt aatggtattt ctattatcat atttgcgggt attttatcaa
    24961 cgttgccagc cagtctaatt caatttggac aaactgcgtt cgttgggcaa gaagatacat
    25021 cattagcatg gttgaaagta ttaggacttt tagtgtcact gatactatta actgtaggtg
    25081 ctatttatgt acttgaagcc gttcgtaaaa ttccgattca atatgctaag aaacaaactg
    25141 ctcaaagatt aggttcacag gcaacttatc tacctttaaa agtgaactca gccggtgtta
    25201 ttcctgtaat ctttgcaatg gcgttcttct tactgcctag aacattaacg ttgttctatc
    25261 cagataaaga atgggcacaa aacattgcga atgctgcaaa tccttcaagt aatgttggta
    25321 tggttgtata tattgtacta attatactat tcacatactt ctatgcattt gtacaagtta
    25381 atccggaaaa aatggctgat aaccttaaga aacaaggtag ctatgttcca ggcattagac
    25441 ctggtgaaca aaccaaaaaa tatattacta aagttttata tcgtttaact tttgtaggtt
    25501 ctatcttctt agccgttatt tcaattctac cgatattagc aactaagttt atgggattac
    25561 cacaatcaat tcagattggt ggtacaagtt tacttatcgt tattggtgta gcgattgaaa
    25621 caatgaaatc tttagaagct caagtatctc aaaaagaata taaaggcttt ggtggtagat
    25681 aattttagga gggcacttat gaatatcatt ttgatgggtt tacctggcgc aggtaaagga
    25741 actcaagcaa gtgaaattgt caagaaattc ccaatacccc acatttcaac tggtgacatg
    25801 ttcagaaaag ctataaaaga agaaactgaa ttaggtaaag aagctaagtc ttatatggac
    25861 cgtggcgaat tagttcctga tgaagtgact gtaggtatcg ttaaggaaag aatttctgaa
    25921 gacgatgcaa aaaaaggctt tttattagat ggcttcccaa gaacaatcga gcaagctgag
    25981 gcattaaata atattatgtc tgagcttgac agaaacattg atgctgtcat caatatcgaa
    26041 gttccggaag aagaattaat gaaccgtctt acaggtcgtc gaatctgtga gtcatgtggt
    26101 acaacgtatc atcttgtatt taatcctccg aaggtcgaag gtatttgtga tatcgatggt
    26161 ggtaaattgt atcaacgaga agatgataat cctgaaacgg tagctaatcg tttgagtgtt
    26221 aatattaaac aatctaagcc tattttagat ttctatgatc aaaaaggtgt attgaaaaat
    26281 attgatggtt caaaagatat tagcgacgtt accaaagatg tcattgatat tttagatcat
    26341 ttgtaataga tcaactttta gcggtctatt ttaagtgagg catgatttaa tcatattaga
    26401 tagatgagag tggcaattaa cgataattaa ttcccgtaat ttttgtttaa cttataaaat
    26461 taatcactga tagattcgta acaataaagt tgtctattga cgattacctt actattgcaa
    26521 aaagggggaa gttaatcaat ggctaaacaa gatgtaattg aattagaagg tactgtatta
    26581 gatactttac cgaacgcaat gtttaaagta gaattagaaa atggtcatga gattttagct
    26641 cacgtaagtg gtaaaatcag aatgaattac attcgtattc tacctggcga caaagtaact
    26701 gttgagatgt ctccgtacga tttaacacgc ggaagaatta cttatcgtta taaataatcg
    26761 tcactccata atatagggag gtataaaaat gaaagtaaga ccatcagtaa aacctatttg
    26821 cgaaaaatgt aaagtcatta aacgtaaagg taaagtaatg gtaatttgtg aaaatccaaa
    26881 acacaaacaa agacaaggtt aataaaagag aggtgtaaat taatatggca cgtattgcag
    26941 gagtagatat tccacgtgaa aaacgcgtag ttatctcatt aacttatata tacggtatcg
    27001 gtacgtcaac tgctcaaaaa attcttgaag aagctaacgt atcagctgat actcgtgtga
    27061 aagatttaac tgatgacgaa ttaggtcgca tccgtgaagt tgtagacggt tataaagtcg
    27121 aaggtgactt acgtcgtgaa actaacttaa atatcaaacg tttaatggaa atttcatcat
    27181 accgtggtat ccgtcaccgt cgtggtttac cagttcgtgg tcaaaaaacg aaaaacaacg
    27241 cgcgtactcg taaaggacca gttaaaacgg tagctaacaa gaaaaaataa taggtaaagg
    27301 aggcaaattt taaatggcac gtaaacaagt atctcgtaaa cgtagagtga aaaagaatat
    27361 tgaaaatggt gtagcacaca tccgttcaac attcaacaac actattgtaa ctatcactga
    27421 tgagttcggt aatgctttat catggtcatc agctggtgca ttaggattca aaggatctaa
    27481 aaaatcaaca ccatttgcag cacaaatggc ttctgaaact gcatctaaat cagctatgga
    27541 gcatggttta aaaacagttg aagtaacagt taaaggacct ggtccaggtc gcgaatcagc
    27601 tattcgtgca ttacaatctg caggtttaga agtaactgcg atcagagacg ttactccagt
    27661 acctcataac ggttgtcgtc caccaaaacg tcgtcgtgta taatttatga tggtattgtt
    27721 acaggtcact gagcaaacat tttaaattaa gtcgacgtat ataaggagga tatttaaatg
    27781 atagaaatcg aaaaacctag aattgagaca attgaaatta gtgaagatgc taaattcggt
    27841 aagttcgttg ttgaaccact agaacgtggc tacggtacta cactaggaaa ctccttacgt
    27901 cgtatcctac tatcttcatt accaggtgca gccgttaagt atattgaaat tgagggagtt
    27961 ttacatgaat tctcagcagt agacaatgta gttgaagatg tttctacaat tattatgaac
    28021 attaaacaat tagcattgaa aatttactct gaagaagata aaactttaga aattgatgta
    28081 cgtgatgaag gcgaagtaac agcaagcgac attacacatg acagtgatgt tgaaatttta
    28141 aacccagagc ttaaaattgc aacagtatct aaaggtggtc acttaaaaat tcgtctagtt
    28201 gctaacaagg gtagaggtta cgcattagca gaacaaaata atactagtga tttaccaatt
    28261 ggtgtaatcc ctgttgattc attgtattca cctgttgaac gtgtgaacta tactgttgaa
    28321 aatacacgtg taggtcaaag cagtgatttt gataaattaa cattggatgt ttggactaat
    28381 ggttcaatca caccacaaga atcagtttca ttagcagcaa aaataatgac tgaacacttg
    28441 aatatcttcg ttggtcttac tgatgaagcg caaaacgctg aaatcatgat tgaaaaagaa
    28501 gaagatcaaa aagaaaaagt attagaaatg tctattgaag aattagactt atctgtacgt
    28561 tcatataact gcttaaaacg cgcaggaatc aattctgttc aagagttagc tgacaaatct
    28621 gaagctgaca tgatgaaagt gcgtaattta ggtcgtaaat ctttagaaga agttaaatac
    28681 aaattagaag atttaggatt aggattaaga aaagaagatt gataaaggag gttaactcat
    28741 gggttacaga aaattaggtc gtacttctga tcaacgtaaa gctatgttac gtgacttagc
    28801 tacatcactt attattagtg aacgtattga aactacagaa gctcgtgcaa aagaagttcg
    28861 cagtgttgtt gagaaattaa tcactttagg taaaaaagga gatttagctt ctcgtcgtaa
    28921 tgcagctaaa actttacgta atgttgaaat cttaaacgaa gatgaaacta cacaaactgc
    28981 acttcaaaaa ttatttggtg aaatcgcaga gcgttacaca gaacgtcaag gtggttacac
    29041 tcgtatcctt aaacaaggcc ctcgtcgcgg tgacggtgct gaatcagtaa ttatcgaatt
    29101 agtataagaa tttgtataaa tacactaact acttttatat aaagcaaatg agtgcaacga
    29161 taatgtttgc cacatacaaa tattgtctag ctcagagtac cccatcaact aaataaagat
    29221 ttaaagcgtg aactcaacaa acttatgatg gggtgcgcgc ttttttattg tataagctat
    29281 cggacttttg aacagaaatg tttacaagtc cgatttcttt atatctactt ttttgagtgt
    29341 gaatctaaaa atatgatcaa gctagtttga tcaatttctc tagaactgaa tgaagtgaaa
    29401 gtactatcgt tgaatcaaac gcggccaacc ctatgttagt aaatcattta ttttattaac
    29461 gtttattcat aattgttaga gaggaataaa tgtccttatc attagttgtt aaacaaccct
    29521 ttaatgatta gaaatgagtc agaaaatact agtaatatat agcaaaccca gtcaatgtca
    29581 tatacagcga tttataaata ttgtaaaata tgatagatac acgaatcgta aaggagagag
    29641 acaagtggag gataagaatt cagttattgt atttaaaaat gtttcatttc aatatcaaag
    29701 tgatgcatcc ttcacattga aagatgtttc ttttaatata cctaaaggtc agtggacatc
    29761 tattgttggt cataacggtt ctggaaaatc tacaattgcc aagttaatga ttggcataga
    29821 gaaagttaaa tctggagaaa ttttttataa taatcaagct ataacagatg ataattttga
    29881 aaagttaaga aaagacatag gaattgtatt tcagaatccg gataatcaat ttgttggttc
    29941 aattgtaaaa tacgatgtgg catttggact cgaaaatcat gcggttccac atgacgaaat
    30001 gcatagaaga gtcagcgaag cacttaaaca agttgatatg ttagaacgtg cagattatga
    30061 acctaatgca ttatctgggg gccagaagca gcgtgtggct atagcaagtg tattagcact
    30121 taacccctct gtcattatat tagatgaggc gacttctatg ttagatcctg atgcacgtca
    30181 aaatttattg gatttagtga gaaaagttaa atcagaacat aatattacaa tcatttctat
    30241 tacgcatgat ttatctgagg cgatggaagc agatcatgtt atcgttatga ataaagggac
    30301 tgtatataaa gaaggtacag cgattgaaat tttcgaccat gcagaagagt taacaacaat
    30361 aggtttagat ttgccattcc caatcaaaat aaatcaaatg ctgggatacc aaacatcatt
    30421 cttaacttat gaagggctgg tggatcaact atgactatac gctttgacaa tgtaagttat
    30481 acctatcaaa aagggacacc ataccagcat caagctattc atgatgttaa tacagaattt
    30541 gaacaaggta aatattacgc catcgttgga caaacgggta gtggtaaatc aacattgata
    30601 caaaatatta atgcactgtt aaagccaact actgggacag ttacagttga tgacattact
    30661 attacacata agaccaaaga taaatatatt agacctgtaa gaaaaagaat tggaatggta
    30721 tttcaatttc ccgaatctca attatttgag gacacagtag agcgtgaaat gatatttgga
    30781 cctaaaaact ttaaaatgaa tttagatgaa gccaaaaact atgcccatcg tctgttgatg
    30841 gatttaggtt tttcaagaga tgtaatgtca caatcaccat ttcaaatgtc aggtggacaa
    30901 atgcgtaaaa tagcgattgt atcgatattg gcaatgaatc ctgatattat cgtggttgat
    30961 gaacccacag caggacttga tccacaaagt aaacgacaag taatgcgatt actaaagtca
    31021 ctacaaacag atgaaaataa ggcaattatc ctaatttcac atgatatgaa tgaagtcgcg
    31081 cgttatgcag atgaagtcat cgttatgaaa gaaggtagta tagtgtcgca aacatcacca
    31141 aaagagctct tcaaagataa agaaaaattg gcggattggc atattggttt gccagaaatt
    31201 gttcagttac aatatgactt tgaacaaaaa catcaaacaa aattaaaaga tattgcctta
    31261 actgaagaag catttgtaag cttgtataag gagtggcaac atgaaaaata aattgattat
    31321 agggcgatat ttaccaatta attcttttgt tcatcatctt gatccaagag caaagttaat
    31381 gtttgttttt ctttttatta tattaatatt tttctgtcat tcaccattaa catatctttg
    31441 ggtgtttgca cttatattat tctttatgag attagcaaaa attcaattat ggttcctaat
    31501 caaaggttta acgccaatat ttttcttttt aatcttcaca ttaatgatgc atatattttt
    31561 aactaaaggt ggatatgtat tagttgaatg gcatggcatc acgattgaaa ctaatggtat
    31621 tttagaaggg ctatatatat cactgcgttt aattggtatt gtgatgattg caacaattat
    31681 gacactatct acaagtccaa ttgatttaac agatgcgttt gaaagattac ttgcaccact
    31741 aaagatgttt aaattaccag ttcatcaatt aagtatgata atgtccattg cgttacgatt
    31801 catcccgacg ttaatggatg agttagataa aatcattttg gcgcaaaagt cgcgtggctc
    31861 tgaaataagt tcagggaaca ttgcaacacg tattaaatca tttattccat tactagtgcc
    31921 actattcatc tcagctttcc aacgtgcaga agaattagcg gttgcaatgg aagttagggg
    31981 ttatgatgcc aatgttcaaa gaacgagcta cagacagctt aaatggcaat tgagagatac
    32041 attatcttta atcatgatta tcccatttgc aattatttta ttcgtattaa aatattcagg
    32101 agtgtaaatt catgcgtata ttagtagaaa ttgcgtatca aggaaataat tttctaggtt
    32161 ttcaaattca acaaaatggt cgaacggtac agcaacaatt tgaaaagcta ttacaacgca
    32221 tgcataaaag acatgtgaga atccatcctt caagtaggac tgatagaggt gtacatgcga
    32281 tacagcaata ctttcatttc gacacagagc tgaatatacc tatgtcacaa tggcaatatg
    32341 caatgaaccg aacgttacct gatgatattt atgtcaacaa tgtagttaca gtcgatgacg
    32401 attttcattg tcgttacgat tgtgttggta aacgctatcg atataaagta tatcaagcac
    32461 aacaccgcga tccatttcaa agtggtttga aaacattcat tcctgaaacg ttggatttag
    32521 ataaaatgaa cagagcggca caacaattca taggtacaca tgattttacg ggtttttgtt
    32581 cgcaaaaaac tgaagtagaa agcaaagtta gaacattata ccaaagtgaa attgtgaaaa
    32641 ccgatgatgg ctttgattat attgtcactg gatctggatt tttatataac atggtgcgtg
    32701 tcttagttgc atttttaata gaagtcggaa aagggcgtca cgaagtatct gatgtaccaa
    32761 aacttcttga atcaaagaat cgtaagaatg taccttttac tgcaccggct gaaggattat
    32821 atttagagaa aatttattta gatgaaaatg agttattaaa agactttggt aacgatatca
    32881 aaatacatcg taaaaaatca ttacaaaatg actgattgcc attgacaaaa agcttgtgaa
    32941 attataagat tattaacggt attgttttat atccacccca cgataagccc cggaaactta
    33001 ttgtgttaca agatatataa gcagaaacga acaacagtta acaaaataaa tgaaattaaa
    33061 cgttttaaaa atgaaacaaa tgaaatcatc tattaggtta tgaaactgtt tatagcttga
    33121 atagaagcat ttatttttta ggaggacaat tattatgcgt caaacattta tggcaaatga
    33181 atcaaacatt gagcgcaaat ggtatgttat cgatgctgaa ggccaaacat taggtcgttt
    33241 atcatcagaa gtagcatcta tcttacgcgg taaaaataaa gcaacttaca caccacacgt
    33301 tgatactggt gattatgtaa tcgttattaa tgcatcaaaa atcgaattta ctggtaacaa
    33361 agaaactgac aaagtttact accgtcactc aaatcaccca ggtggtatca aatcaatcac
    33421 tgctggtgaa ttaagaagaa ctaatccaga acgtttaatt gaaaactcaa ttaaaggtat
    33481 gttaccaagc actcgtttag gcgaaaaaca aggtaaaaaa ttatttgtat atggtggcgc
    33541 tgaacatcca cacgctgcac aacaaccaga aaactacgaa ttacgtggtt aattagaagg
    33601 aggaaatgac tttggcacaa gttgaatata gaggcacagg ccgtcgtaaa aactcagtag
    33661 cacgtgtacg tttagtacca ggtgaaggta acatcacagt taataaccgt gacgtacgcg
    33721 aatacttacc attcgaatca ttaattttag acttaaacca accatttgat gtaactgaaa
    33781 ctaaaggtaa ctatgatgtt ttagttaacg ttcatggtgg tggtttcact ggacaagctc
    33841 aagctatccg tcacggaatc gctcgtgcat tattagaagc agatcctgaa tacagaggtt
    33901 ctttaaaacg cgctggatta cttactcgtg acccacgtat gaaagaacgt aaaaaaccag
    33961 gtcttaaagc agctcgtcgt tcacctcaat tctcaaaacg ttaagctgca actgttgcga
    34021 acttgttcgt ttacagatgg agttcgcaaa ttgcgtcagc aatttgtgtt caactgaaga
    34081 aacgtttaca acactctttg gattattcca aagagtgttt tttgtatttt tatagagtca
    34141 tttagacacc atttagttac ccctagggaa ctaatttgtt tcaattctgc ttaaaattca
    34201 atgatatgaa actttaggaa accatatgaa atgaccttgt ttttctttgg gattcgtacg
    34261 acaagtagat agaggttgta atgatattcg atagatatcg tattgaatgt ttgcaaactg
    34321 aggttgaagg acagctttcg agtaaaaagg aaaattttcg agtaactcaa aagaaaaata
    34381 taaaaaaatc atgagaaacc aatgatatca agcgttttgt gagctttcga gtaaagagga
    34441 taattttcga gtaactcaaa agaaaatact aaaaaatcat gggaaatcat tgatatcaag
    34501 ggttttgtga gctttcgagt aaagaggcgg attttcgagt agtattattt aataagaatt
    34561 acaggttaaa taaaatacct actaatcaga aggtgacaga gattgtagaa tttctatcag
    34621 atggaaagtt agcaagtagg ctagaaattc aagaaacaat gggacttcaa aaaagttata
    34681 catctgagtt aattgctgaa ctgaagaagt tagagattat cggatctgaa ggtagaggtc
    34741 tgggaactag atattattta attagagact aataaattca tcaagtgata accggacgat
    34801 aacctattga tatacataaa aacattgata aatcaatata tttacgaccg gacgataacc
    34861 ggacgatacg agagtatata ccaccggaca gcagtttatt tagttaccct ttagttaccc
    34921 cggagtgata tttggtgcaa tactatgcaa tcgaaaaaat cagaaatcct ataatatcat
    34981 gccttaagac aatatatgca actatacgaa accctaaagt aaatcaggtc ttaaagcagc
    35041 tcgtcgttca cctcaattct caaaacgtta attgtcggac gatatataca caaaacacct
    35101 cgatgttatg tcgaggtgtt tttttgcgtt tgtgtgggga atatgaaaag tatgtagtat
    35161 aaatgaacgt tcctttattt aatgtctcag gaatagaata aattcaacta tgaattattg
    35221 atttacacgt ataatttgta catgcttaca caatcaaaag tgtacattat taaattgtca
    35281 tttagcttgc ggaggacgga cgataaccgg tcgataatgt tctaatcata gtggttcaag
    35341 ggaaagggct atcggatgaa aaccggacga taactcaaga accatgattt aagagtgtta
    35401 tcaaaataag gagtgactta attgaaagat tctatatttt ggaagaaagc ttttattcct
    35461 gtttatttta ttgttgcgat gctggtgttt ctacttttta ggttttatat taaaacagac
    35521 aacttttcta tgtatttaat gattatcttc ttaatttgtt taggaactgc atctatcatt
    35581 tataactata aaaacaatcg ataaatataa gtatgaaatg aataagtcac aattagatct
    35641 aagtcttgct gtggcttttt ttatcgaaat gacaatttca tgttgctata aaacagcatt
    35701 ttcaaaccgt cataaaacaa cattttcagc ccgtcataaa acaacatttt cagcccgcca
    35761 taaaacgaca atttcaaacc gccattgact aaacacctca ttcccaaata tgctaacaat
    35821 cctccacatc aatcaatccg acattcttca ttatcacact gcacaaaatc tactcatgca
    35881 tttttggaat acttagtatt acaaataacg atttttattc atcttacaaa ggatatataa
    35941 tgtactgaag gcaattttta tgtattacaa atctaatcgt acatgtaaaa ttttgaaaaa
    36001 catcattaat tttgcgtaac tatcattaga ttacaaatca caatgcaatt acatgtaata
    36061 cacatctaca catcacattt gaagggaaat gaatataaat gactgataaa aagtacactg
    36121 cagccgatat ggttattgat actttaaaaa ataatggggt agaatatgtt tttggtattc
    36181 cgggtgcaaa gatagactat ctatttaatg ctttaattga tgatggtcct gaacttattg
    36241 tcacgcgtca tgaacaaaat gccgcaatga tggcgcaagg tattggaaga ttaacaggca
    36301 aacctggtgt agtacttgtt acaagtggcc ctggtgtaag taatttaacg actggactat
    36361 taacagctac atctgaaggg gatcctgtat tagcgttagg tggccaagtg aaacgtaatg
    36421 atttattacg attaacgcat caaagtattg ataatgctgc gctattaaaa tattcatcaa
    36481 aatacagtga agaagtacaa gatcctgaat cattatcaga agttatgaca aatgcaattc
    36541 gaattgctac ttcaggaaaa aatggcgcaa gttttattag tattccgcaa gacgttattt
    36601 cttcaccagc tgaatctaaa gcgatatcac tttgccaaaa accaaattta ggagtaccga
    36661 gtgaacaaga tattaatgat gtcattgaag cgattaaaaa tgcatcattt cctgttttat
    36721 tagctggtat gagaagttca agtgcagacg aaacaaatgc cattcgcaaa ttagttgagc
    36781 gcacgaattt accagttgta gaaacattcc aaggtgcagg tttaattagt cgtgaattag
    36841 aaaatcattt ctttgggcga gtgggtttat tccgtaatca agttggtgat gaattattac
    36901 gtaaaagtga tttagttgtt acaatcggtt atgatcctat agaatatgag gctagtaact
    36961 ggaataaaga attagatacg caaataatca atattgacga agttcaagct gaaattacta
    37021 attatatgca accgaaaaaa gagttgattg gtaatattgc taaaacgatt gaaatgattt
    37081 ctgacaaagt agatgaacca tttataaatc aacaacattt agatgaatta gaacagttaa
    37141 gagcgcatat tgatcaagaa actggtatta aagcgacaca tgaagaaggc atcttacacc
    37201 cagttgaaat tatagaatct atgcaaaagg tattaactga tgaaacaaca gtgacagttg
    37261 atgttggtag tcactatatt tggatggcac gtaatttcag aagttacaat ccaagacatt
    37321 tattatttag taacggtatg caaacacttg gtgtagcatt accgtgggca atttcagctg
    37381 cacttgtacg ccctaatacg caagttgtat ccgttgctgg cgatggaggc tttttatttt
    37441 catcacaaga tttagaaacg gccgtacgta aaaatctaaa cattattcaa cttatttgga
    37501 acgatggaaa atataacatg gttgaattcc aagaagaaat gaaatataaa cgttcatcag
    37561 gtgtcgactt cggtcctgta gatttcgtaa aatatgctga atcatttggc gcaaaaggtt
    37621 tacgagttac taatcaagaa gaattagaag cggcaattaa agaggggtat gaaacagatg
    37681 gcccagtatt aattgatata cctgtaaatt acaaagataa tatcaaactt tcaacaaata
    37741 tgttacctga cgtatttaac taaaatacag ataaatgtta atgaggagtg ggaaatttta
    37801 tggctagtgt tttatatcaa catggtacat taggcacatt aatggcaggt ttattaaaag
    37861 gaactgcatc aataaatgaa ttattgcagc atggtgattt aggtatcgct acactaacag
    37921 gttcaaatgg tgaggtcatc tttttagatg gaaaagccta tcatgcaaat gaacacaaag
    37981 aatttgtgga attaaaaggt gatgagttaa caccatatgc aactgtaact aaattcgtag
    38041 cagatacaag ctatgaaacg aaagataaat cttctgaagc agtttttgca gaaattaagg
    38101 aaaagatgtt gagtgaaaat ttattttctg cagtaaaaat ttcaggctta tttaaaaaaa
    38161 tgcatgtacg tatgatgccg gctcaagagc caccatatac acgtttaatt gattcagcta
    38221 gaagacaacc tgaacaaact gaaacgtacg tcaaaggttc agttgttggt ttctttacac
    38281 cagaattatt ccatggtatt ggatcagcag gtttccatgt acactttgcc aatgatgatc
    38341 gtaactttgg tgggcatgtc ttagactttg aagtagaaga tgttaaagta gaaatccaaa
    38401 atattgaaac atttgaacag cattttccaa ttcatgatga agatttcact aatgcaaata
    38461 ttgactataa agatatttca gatgaaatta gagaagctga ataacattaa ctgtataatg
    38521 aatgcgtaaa tgtattatca gtttatgaaa attgacttca taatgcgcga tttagaaatg
    38581 gtagtttgtt aatatgatta atcatgacta caatagaaca aatatattta tgattacgtc
    38641 tatgtaataa aataaatccc ttcactatta gctgtagtga ggggatttat ttaggtttca
    38701 gatatttgag atttgctgtt atgtttagat tataaattga ggaagacaat aatatagaat
    38761 ttactttagt ataggccaat ctattgtctg gagaagcaaa caacaataat ttgcaagttc
    38821 aataactaaa aagacaaacg ccaatttttc agcgcttgcc ctataaaact attttcaaat
    38881 tattatttaa ctacaatttg tatttgtttg attgtacttg gatcaattga atttttagca
    38941 tcgtcatttt tctttagatt cactactttt ttattaccat cattcaaagt aagtgtataa
    39001 gttgctgttt gtgcattatt aattttttct gttgtaacac cacgttgaga agctaattca
    39061 ttttttactt tactgtcaat ttcttgataa agtacctttt tattttctgg aaaaacaaag
    39121 taagttcgat gtaatgcagt aataccatct actgaaatcg tgtaaggaac agtgtgataa
    39181 ccatccattg tcatttcttt atagccgtta ttactatctg cagatgcttc gtgactaggt
    39241 aatgcagctc cgaccgttga taataatccc aatgctaaag tggcagtaac aaatgatttt
    39301 agtttcatat ctcaatctcc ttttaaaatt gtggtatatt acactattta tataacacgt
    39361 ttcataaagt ttaaaacata ctttttgatt tttttatatt tttgattttt agtaatagtt
    39421 tttaacgagg gatatgaccc gtgtattttg aaaagtggta agcaatgttt ttagtttata
    39481 gggattcatt agttgtatat aataagcatg aaaagcatat agtcgatctt atacttttaa
    39541 ttgagactta tagtgtattt aagttataga taaattcatt aaaatattat ggaggaatga
    39601 gtatgtcaaa gcgtctatta ttgtttgatt ttgatgaaac atattttaaa cataatacaa
    39661 atgaagaaga tttaagtcat ttaagagaaa tggaaaaatt gttggaaaaa ctaactaata
    39721 ataatgaagt gatcactgct gtattgacag gaagtacatt tcaaagtgtc atggataaaa
    39781 tggatcaagt taatatgacg tttaaaccat tacatatctt ttctgatttg agttctaaaa
    39841 tgttcacttg gaataatggc gaatatgttg aatcagaaac atataaaaag aaagtcttga
    39901 gcgaaccatt tttatttgaa gatattgagg atatattacg tcatatttct gcgcaatata
    39961 atgtcgaatt tattccacaa agagcatttg aaggtaatga aacacattat aatttttatt
    40021 ttcattcaac aggtaatcac aacaatgata ggcgtattct agaagcactt gtcagatacg
    40081 caaatgacca aaactataca gcgagattta gtcgaagcaa tccattagct ggtgatcctg
    40141 aaaatgcgta tgatattgac tttacaccgt caaatgctgg aaaattatat gctactcaat
    40201 ttctgatgaa gaaatataat atacccgtaa aatcaatatt aggttttgga gatagcggca
    40261 atgacgaagc atatttaagc tatcttgaac atgcctattt gatgtcgaat agtagagatg
    40321 aagcattaaa gcagaaattt agattaacaa aatatccata ttatcaaggg ataacactgc
    40381 atgttaaaga atttgttgaa ggtaagtatg attattgatt ggttgagtga tatgtatact
    40441 ttttaagtgc aaatattaat atttaaaatt tgaggaatga ttagataata tggtgaatcg
    40501 actacacttt agttttaaaa aatttatttg taagttgatt tgtctaacta agtgtagacg
    40561 attcatagtt taatcaatat atttgaatac tttgtaaatt aaataaacat ttaatattga
    40621 tactattgaa gatatagagg gctttgatct ctattggctt ttttcatcga ttaattcaat
    40681 ccattttttg gatactgctt cgtaagaaaa gtttgtcttt attgttgttc tagcattttc
    40741 agaaaactga gatatcaatg tttctttttt taatacatga attatttttt ttgccaattc
    40801 attaatatta tccttctttt ttgttattat tccatttata ttattttgga ttatagattt
    40861 aggaccataa ttaataggat atgaaataac tggaacaccg taactcatgg cctctagtaa
    40921 actcattggg agaccttcat aatctgatgt cagtacactt agagaggttt ttgtataaag
    40981 agcacgtatg tcaaatgcgt aatcatggat gaacacatta tcttgcaatt taagtttttt
    41041 aataagtttt gtgtaatttt ctttttgcgt accggtaccg tatatgtgta acttaacatc
    41101 ctttactttt tttcttacaa tatttatagc ggaaatgata tgtgtgatat ttttcatagc
    41161 tacaaatctt tgtaatacaa taatagaatt tttttctcga atattttgtg ttaaattttc
    41221 ttcaaaagat atagcattag gaataacctt tatattattg taatcaccaa attgttttat
    41281 aatgtcatct ttttgttctt tagttaatac aactaaagta tctaactttt taaggtttag
    41341 taacatgttt ctgaactctg gtttaatctc agcgccatag gagtaagggg acatgaaatg
    41401 attcgtatgt atagtaaata tttttttgat atcattattc tgcatgttct ctactttatg
    41461 ataagtacct ataccgtcta gtattaatac gtcgtttgaa gttaagtttt tatccaacca
    41521 atatgaatgg aacgctttat tatttttaaa gtttaatact tctttgctgt ttttatcaaa
    41581 ataaaatata ttgactatat tttcgttatc cccataccaa tatgataagt aacaaaagcc
    41641 gtcatttgat aaatatttaa cttcactcat tttattttta tcgttgaaag ttatttcttt
    41701 tcgaagtatt ttattttgaa aaacatgtct tttaaagcgt tttcgatttt gaaaataatc
    41761 aatgtgtgat aattgaccat ttatccattt tttatacatt atgtaatggt tatctttaaa
    41821 gtatctagca tatttttttt cttcatattc gtctttttga atataaaagc cttcttgatt
    41881 taaaagattt aatttttcaa atgtttgcat atttttattt tcgatttctg tattttcaac
    41941 atcatacagc atcttcaatt cttcgtataa gttaacaatt tttacttgtt tatgtaaccg
    42001 tcctgaattt gttagcttag ctcttaatgg atcatagttt ttgtcatcca aagttaataa
    42061 ggcacatgaa tagccttttt cagctaaaag tttagatcta tttaacataa ctgatgttat
    42121 accacctgct ttttctggta ttgaatacat taacatataa gtagtcataa tttcctccta
    42181 tttcttgcac ttgataatta attctccgta attatagtag taatcttcat ctacatatat
    42241 tttggagaac gtttctaaag aactgtaatt ttctatttca gcaactataa tatgatttaa
    42301 tgctaaataa ggattagagc cattaaaaac aattttg
//
LOCUS       DAHHOJ010000008        41883 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.008, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000008 DAHHOJ010000000
VERSION     DAHHOJ010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41883
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.008"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(159..1415)
                     /locus_tag="NBH32_000357"
     CDS             complement(159..1415)
                     /locus_tag="NBH32_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001569336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3578 domain-containing protein"
                     /protein_id="HCD1513994.1"
                     /translation="MIDNFMQVLKLIKEKRTNNVVKKSDWDKGDLYKTLVHDKLPKQL
                     KVHIKEDKYSVVGKVATGNYSKVPWISIYDENITKETKDGYYLVYLFHPEGEGIYLSL
                     NQGWSKISDMFPRDKNAAKQRALTLSSELNKYITSNEFNTGRFYYAENKDSSYDLKND
                     YPSGYSHGSIRFKYYDLNEGFTEEDMLEDLKKFLELFNELASKVTKTSYDSLVNSIDE
                     IQEDSEIEEIRTAQKDKTLKEVEAPKGIIPKYKKGVSKTTKNDSEIEKSNKENKLTGK
                     VGEKLALNYFNELIDNKIDEDKKEQFRNILNDNPGSQHGHGYDLVAFDPTNTDKAVEK
                     FIEIKTSTSSSIEEPFFMSLNEMFAMKEYKQKYLILRIFNVSGKEPQFYFIDPYANYS
                     EFKGVDDLIDKVFNVEAIQYKVFGEK"
     gene            complement(1737..2216)
                     /gene="rlmH"
                     /locus_tag="NBH32_000358"
     CDS             complement(1737..2216)
                     /gene="rlmH"
                     /locus_tag="NBH32_000358"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020363758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCD1513995.1"
                     /translation="MKITILAVGKLKEKYWKQAIAEYEKRLGPYTKIDIIEVPDEKAP
                     ENMSDKEIEQVKEKEGQRILAKIKPQSTVITLEIQGKMLSSEGLAQELNQRMTQGQSD
                     FVFVIGGSNGLHKDVLQRSNYALSFSKMTFPHQMMRVVLIEQVYRAFKIMRGEAYHK"
     gene            complement(2584..4902)
                     /gene="adsA"
                     /locus_tag="NBH32_000359"
     CDS             complement(2584..4902)
                     /gene="adsA"
                     /locus_tag="NBH32_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored adenosine synthase AdsA"
                     /protein_id="HCD1513996.1"
                     /translation="MKALLLKTSVWLVLLFSAMGLWQVSSAAEQHTPMKAHAVTTIDK
                     ATTDRQLVLPTKEAAHQSGEEAATNVSASAQGTADDTNNKVTSNAPSNKPSTAVSTTV
                     NETHDVDAQQASTQKPTQSATFKLSNAKTASLSPRMFAANAPQTTTHKILHTNDIHGR
                     LAEEKGRVIGMAKLKTVKEQEKPDLILDAGDAFQGLPLSNQSKGEEMAKAMNAVGYDA
                     MAVGNHEFDFGYDQLKKLEGMLDFPMLSTNVYKDGKRAFKPSTIVTKNGIRYGIIGVT
                     TPETKTKTRPEGIKGVEFRDPLQSVTAEMMRIYKDVDTFVVISHLGIDPSTQETWRGD
                     YLVKQLSQNPQLKKRITVIDGHSHTVLQNGQIYNNDALAQTGTALANIGKVTFNYRNG
                     EVSNIKPSLINVKDVENVTPNKALAEQINQADQTFRAQTAEVIIPNNTIDFKGERDDV
                     RTRETNLGNAITDAMEAYGVKNFSKKTDFAVTNGGGIRASIAKGKVTRYDLISVLPFG
                     NTIAQIDVKGSDVWTAFEHSLGAPTTQKDGKTVLTANGGLLHISDSIRVYYDMNKPSG
                     KRINAIQILNKETGKFENIDLKRVYHVTMNDFTASGGDGYSMFGGPREEGISLDQVLA
                     SYLKTANLAKYDTTEPQRMLLGKPAVSEQPAKGQQGSKGSESGKDTQPIGKDKVMNPA
                     KQPATGKVVLLPTHRGTVSSGAEGSDCALEGTAVSSKSGKQLTKMSASKGSGHEKQLP
                     KTGTNQSSSPAAIFVLVAGIGLIATVRRRKAS"
     gene            complement(5128..5928)
                     /locus_tag="NBH32_000360"
     CDS             complement(5128..5928)
                     /locus_tag="NBH32_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1513997.1"
                     /translation="MSRLIRMSVLASGSTGNATFVENEKGSLLVDVGLTGKKMEELFS
                     QIDRNIQDLNGILVTHEHIDHIKGLGVLARKYQLPIYANEKTWQAIEKKDSRIPMDQK
                     FIFNPYETKSIAGFDVESFNVSHDAIDPQFYIFHNNYKKFTILTDTGYVSDRMKGMIR
                     GSDAFIFESNHDVDMLRMCRYPWKTKQRILGDMGHVSNEDAGHAMTDVITGNTKRIYL
                     SHLSQDNNMKDLARMSVGQVLNEHDIDTEKEVLLCDTDKAIPTPIYTI"
     gene            complement(6324..7112)
                     /locus_tag="NBH32_000361"
     CDS             complement(6324..7112)
                     /locus_tag="NBH32_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HCD1513998.1"
                     /translation="MNWKLTKTLFIFVFILVNIVLVSIYVNKVNRSHINEVESNNEVN
                     FQQEEIRVPASILNKSVKGIQLEQITGRSKDFSSKAKGDSDLSTSDSGKLLNANISQS
                     VKVSDNNLKDLKDYVNKRVFKGAEYQLSEISSDSVKYEQTYDDFPILNNSKAMLNFKI
                     EDNKATSYKQSMMDDIKPTDGADKKHQVISVRKAIEALYYNRYLKKGDEVINARLGYY
                     SVVNETNVQLLQPNWEIKVKHDGKDKTNTYYVEATNNNPKIINH"
     gene            complement(7113..8447)
                     /locus_tag="NBH32_000362"
     CDS             complement(7113..8447)
                     /locus_tag="NBH32_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001060131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1513999.1"
                     /translation="MNNKEHIKSVILALLVLMSVVLTYMVWNFSPDIANVDNTDSKKS
                     ETKPLTTPMTAKMDTTITPFQIIHSKNDHPEGTIATVSNVNKLTKPLKNKEVKSVEHV
                     RRDHNLMIPDLSSDFTLFDFTYDLPLSTYLGQVLNMNAKVPNHFNFNRLVIDHDADDN
                     IVLYAISKDRHDYVKLTTTTKNDHFLDALAAVKKDMQPYTDIITNKDTIDRTTHVFAP
                     SKPEKLKTYRMVFNTISVEKMNAILFDDSTIVRSSKSGVTTYNNNTGVANYNDKNEKY
                     HYKNLSEDEASSSKMEETIPGTFDFINGHGGFLNEDFRLFSTNNQSGELTYQRFLNGY
                     PTFNKEGSNQIQVTWGEKGVFDYRRSLLRTDVVLNSEDNKTLPKLESVRSSLANNSDI
                     NFEKVTNIAIGYEMQDNSDHNHIEVQINSELVPRWYVEYDGEWYVYNDGRLE"
     gene            complement(8440..10266)
                     /gene="walK"
                     /locus_tag="NBH32_000363"
     CDS             complement(8440..10266)
                     /gene="walK"
                     /locus_tag="NBH32_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HCD1514000.1"
                     /translation="MKWLKQLQSLHTKLVIVYVLLIIIGMQIIGLYFTNNLEKELLDN
                     FKKNITQYAKQLEISIEKVYDEKGSVNAQKDIQNLLSEYANRQEIGEIRFIDKDQIII
                     ATTKQSNRSLINQKANDSSVQKALSLGQSNDHLILKDYGGGKDRVWVYNIPVKVDKKV
                     IGNIYIESKINDVYNQLNNINQIFIVGTAISLLITVILGFFIARTITKPITDMRNQTV
                     EMSRGNYTQRVKIYGNDEIGELALAFNNLSKRVQEAQANTESEKRRLDSVITHMSDGI
                     IATDRRGRIRIVNDMALKMLGMAKEDIIGYYMLSVLSLEDEFKLEEIQENNDSFLLDL
                     NEEEGLIARVNFSTIVQETGFVTGYIAVLHDVTEQQQVERERREFVANVSHELRTPLT
                     SMNSYIEALEEGAWKDEELAPQFLSVTREETERMIRLVNDLLQLSKMDNESDQINKEI
                     IDFNMFINKIINRHEMSAKDTTFIRDIPKKTIFTEFDPDKMTQVFDNVITNAMKYSRG
                     DKRVEFHVKQNPLYNRMTIRIKDNGIGIPINKVDKIFDRFYRVDKARTRKMGGTGLGL
                     AISKEIVEAHNGRIWANSVEGQGTSIFITLPCEVIEDGDWDE"
     gene            complement(10279..10980)
                     /locus_tag="NBH32_000364"
     CDS             complement(10279..10980)
                     /locus_tag="NBH32_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1514001.1"
                     /translation="MARKVVVVDDEKPIADILEFNLKKEGYDVYCAYDGNDAVDLIYE
                     EEPDIVLLDIMLPGRDGMEVCREVRKKYEMPIIMLTAKDSEIDKVLGLELGADDYVTK
                     PFSTRELIARVKANLRRHYSQPAQDTGNVTNEITIKDIVIYPDAYSIKKRGEDIELTH
                     REFELFHYLSKHMGQVMTREHLLQTVWGYDYFGDVRTVDVTIRRLREKIEDDPSHPEY
                     IVTRRGVGYFLQQHE"
     gene            complement(11600..11672)
                     /locus_tag="NBH32_000365"
     tRNA            complement(11600..11672)
                     /locus_tag="NBH32_000365"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11637..11639),aa:Asp,seq:gtc)
     gene            complement(11680..11754)
                     /locus_tag="NBH32_000366"
     tRNA            complement(11680..11754)
                     /locus_tag="NBH32_000366"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11719..11721),aa:Glu,seq:ttc)
     gene            complement(12183..13466)
                     /locus_tag="NBH32_000367"
     CDS             complement(12183..13466)
                     /locus_tag="NBH32_000367"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCD1514002.1"
                     /translation="MSSIVVVGTQWGDEGKGKITDFLAEQSDVIARFSGGNNAGHTIQ
                     FGGETYKLHLVPSGIFYKDKLAVIGNGVVVDPVALLKELDGLNERGIPTSNLRISNRA
                     QVILPYHLVQDEYEERLRGDNKIGTTKKGIGPAYVDKVQRIGIRMADLLEKETFERLL
                     KSNIEYKQAYFKGMFNETCPSFDDIFEEYYAAGQRLKEFVTDTSKILDDAFVADEKVL
                     FEGAQGVMLDIDHGTYPFVTSSNPIAGNVTVGTGVGPTFVSKVIGVCKAYTSRVGDGP
                     FPTELFDEDGHHIREVGREYGTTTGRPRRVGWFDSVVLRHSRRVSGITDLSINSIDVL
                     TGLDTVKICTAYELDGKEITEYPANLDQLKRCKPIFEELPGWTEDVTSVRTLEELPEN
                     ARKYLERISELCNVQISIFSVGPDREQTNLLKELW"
     gene            complement(13744..15144)
                     /gene="dnaB"
                     /locus_tag="NBH32_000368"
     CDS             complement(13744..15144)
                     /gene="dnaB"
                     /locus_tag="NBH32_000368"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCD1514003.1"
                     /translation="MDRMYEQNQMPHNNEAEQSVLGSIIIDPELINTTQEVLLPESFY
                     RGAHQHIFRAMMHLNEDNKEIDVVTLMDQLSSEGTLNEAGGPQYLAELSTNVPTTRNV
                     QYYTDIVSKHALKRRLIQTADSIANDGYNDELELDAILSDAERRILELSSSRESDGFK
                     DIRDVLGQVYETAEELDQNSGQTPGIPTGYRDLDQMTAGFNRNDLIILAARPSVGKTA
                     FALNIAQKVATHEDMYTVGIFSLEMGADQLATRMICSSGNVDSNRLRTGTMTEEDWSR
                     FTIAVGKLSRTKIFIDDTPGIRINDLRSKCRRLKQEHGLDMIVIDYLQLIQGSGSRAS
                     DNRQQEVSEISRTLKALARELECPVIALSQLSRGVEQRQDKRPMMSDIRESGSIEQDA
                     DIVAFLYRDDYYNRGGDEDDDDDGGFEPQTNDENGEIEIIIAKQRNGPTGTVKLHFMK
                     QYNKFTDIDYAHADMM"
     gene            complement(15176..15622)
                     /gene="rplI"
                     /locus_tag="NBH32_000369"
     CDS             complement(15176..15622)
                     /gene="rplI"
                     /locus_tag="NBH32_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCD1514004.1"
                     /translation="MKVIFTQDVKGKGKKGEVKEVPVGYANNFLLKKNYAVEATPGNL
                     KQLELQKKRAKQERQQEIEDAKALKETLSNIEVQVSAKTGEGGKLFGSVSTKQIAEAL
                     KAQHDIKIDKRKMDLPNGIHSLGYTNVPVKLDKEVEGTIRVHTVEQ"
     gene            complement(15619..17586)
                     /gene="gdpP"
                     /locus_tag="NBH32_000370"
     CDS             complement(15619..17586)
                     /gene="gdpP"
                     /locus_tag="NBH32_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP phosphodiesterase GdpP"
                     /protein_id="HCD1514005.1"
                     /translation="MNRQSTKKALLIPFVIMIITAIVLMGVWFIFNSLIALIASIVLV
                     VMIIVSIVLFRQALMKMDSYVDGLSAQISTTNNKAIKHLPIGIIVLDENDHIEWVNQF
                     MTDHMEANVISESVNEVFPNILKQLDRVKSVEIEYNQYHFQVRYSENDHCLYFFDITE
                     QVQTNELYENSKPIIATLFLDNYDEITQNMNDTQRSEINSMVTRVISRWATEYNIFFK
                     RYSSDQFVAYLNQKILADLEESKFDILSQLREKSVGYRAQLTLSIGVGEGTENLIDLG
                     ELSQSGLDLALGRGGDQVAIKSINGNVRFYGGKTDPMEKRTRVRARVISHALKDILAE
                     GDKVIIMGHKRPDLDAIGAAIGVSRFAMMNNLEAYIVLNETDIDPTLRRVMNEIDKKP
                     ELRERFITSDDAWDMMTSKTTVVIVDTHKPELVLDENVLNKANRKVVIDHHRRGESFI
                     SNPLLIYMEPYASSTAELVTELLEYQPTEQRLTRLESTVMYAGIIVDTRNFTLRTGSR
                     TFDAASYLRAHGADTILTQHFLKDDVDTYINRSELIRTVKVEDNGIAIAHGSDDKIYH
                     PVTVAQAADELLSLEGIEASYVVARREDNLIGISARSLGSVNVQLTMEALGGGGHLTN
                     AATQLKGVTVEEAIAQLQQAITEQLSRSEDA"
     gene            complement(17601..18539)
                     /locus_tag="NBH32_000371"
     CDS             complement(17601..18539)
                     /locus_tag="NBH32_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YybS family protein"
                     /protein_id="HCD1514006.1"
                     /translation="MGVAYVFSKIQPKATIIATIALVFVALALYLVPGLGLIFALFAT
                     IPGIVLWNKSIQSFGISALITVIITTVLGNTFVLSAIILVLIASLIIGQLLKERTSKE
                     RILYVTTVAMSLISLIAFMLLQTFGRIPPSASIVKPFKQTLHEAITMSGADANMTQIL
                     EEGFRQATVQLPGFIIIITFLIVLINLIVTFPILRKFKIATPVFKPLFAWQMSGILLW
                     IYIIVIICLLFTGQPSVFQSILLNFQLVLSLVMYIQGLSVIHFFGKAKGLPNAITILL
                     LIIGTILTPTTHIVGLLGVIDLSLNLKRIMKNNSKK"
     gene            complement(18834..19802)
                     /locus_tag="NBH32_000372"
     CDS             complement(18834..19802)
                     /locus_tag="NBH32_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HCD1514007.1"
                     /translation="MTNYTVDTLNLGKFITESGEVIDNLRLRYEHVGYHGQPLVVVCH
                     ALTGNHLTYGTDDYPGWWREIIDGGYIPIHDYQFLTFDVIGSPFGSSSPLNDPHFPKK
                     LTLRDIVRANERGIQALGYDKINILIGGSLGGMQAMELLYNQQFEVDKAIILAATSRT
                     SSYSRAFNEIARQAIHLGGKEGLSIARQLGFLTYRSSKSYDERFTPDEVVAYQQHQGN
                     KFKEHFDLNCYLTLLDVLDSHNIDRGRTDVTHVFKNLETKVLTMGFIDDLLYPDDQVR
                     ALGERFKYHRHFFVPDNVGHDGFLLNFSTWAPNLYHFLNLKHFKRK"
     regulatory      complement(19863..19961)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(20166..20495)
                     /locus_tag="NBH32_000373"
     CDS             complement(20166..20495)
                     /locus_tag="NBH32_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HCD1514008.1"
                     /translation="MITHMNMLILILLCGIVTLLIRIIPFIMISKVQLPDVVVRWLSF
                     IPITLFTALVIDSIIQQTPHGEGYTLNIPYIIALIPTVILSIITRSLTITIISGIVIM
                     AALRFFF"
     gene            complement(20492..21187)
                     /locus_tag="NBH32_000374"
     CDS             complement(20492..21187)
                     /locus_tag="NBH32_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HCD1514009.1"
                     /translation="MATHLSFRQGVQECIPTLLGYAGVGISFGIVASSQNFSILEIIL
                     LCLVIYAGAAQFIMCALFIAGTPISAIVLTVFIVNSRMFLLSMSLAPNFKTYGFWNRV
                     GLGSLVTDETFGVAITPYLKGEAINDRWMHGLNITAYLFWAISCVAGALFGEYISNPQ
                     TLGLDFAITAMFIFLAIAQFESITKSRLRIYIVLIIAVIVMMLSLSMFMPSYLAILIA
                     ATISAALGVMMER"
     gene            complement(21838..23124)
                     /gene="serS"
                     /locus_tag="NBH32_000375"
     CDS             complement(21838..23124)
                     /gene="serS"
                     /locus_tag="NBH32_000375"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCD1514010.1"
                     /translation="MLDIRLFRNEPDTVKSKIELRGDDPKVVDEILELDEQRRKLISA
                     TEEMKARRNKVSEEIALKKRNKENADDVIAEMRTLGDDIKEKDSQLNEIDNKMTGILC
                     RIPNLISDDVPQGESDEDNVEVKKWGTPREFSFEPKAHWDIVEELKMADFDRAAKVSG
                     ARFVYLTNEGAQLERALMNYMITKHTTQHGYTEMMVPQLVNADTMYGTGQLPKFEEDL
                     FKVEKEGLYTIPTAEVPLTNFYRNEIIQPGVLPEKFTGQSACFRSEAGSAGRDTRGLI
                     RLHQFDKVEMVRFEQPEDSWNALEEMTTNAEAILEELGLPYRRVILCTGDIGFSASKT
                     YDLEVWLPSYNDYKEISSCSNCTDFQARRANIRFKRDKAAKPELAHTLNGSGLAVGRT
                     FAAIVENYQNEDGTVTIPEALVPFMGGKTQISKPVK"
     misc_binding    complement(23214..23430)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23503..25017)
                     /gene="hutH"
                     /locus_tag="NBH32_000376"
     CDS             complement(23503..25017)
                     /gene="hutH"
                     /locus_tag="NBH32_000376"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HCD1514011.1"
                     /translation="MTLYLDGETLTIEDIKSFLQQQSKIEIIDDALERVKKSRAVVER
                     IIENEETVYGITTGFGLFSDVRIDPTQYNELQVNLIRSHACGLGEPFSKEVALVMMIL
                     RLNTLLKGHSGATLELVRQLQFFINERIIPIIPQQGSLGASGDLAPLSHLALALIGEG
                     KVLYRGEEKDSDDVLRELNRQPLNLQAKEGLALINGTQAMTAQGVISYIEAEDLGYQS
                     EWIAALTHQSLNGIIDAYRHDVHAVRNFQEQINVAARMRDWLEGSALTTRQAEIRVQD
                     AYTLRCIPQIHGASFQVFNYVKQQLEFEMNAANDNPLIFEEANETFVISGGNFHGQPI
                     AFALDHLKLGVSELANVSERRLERLVNPQLNGDLPAFLSPEPGLQSGAMIMQYAAASL
                     VSENKTLAHPASVDSITSSANQEDHVSMGTTAARHGYQIIENARRVLAIECVIALQAA
                     ELKGVEGLSPKTRRKYEEFRSIVPSITHDRQFHKDIEAVAQYLKQSIYQTTACH"
     gene            25343..26155
                     /locus_tag="NBH32_000377"
     CDS             25343..26155
                     /locus_tag="NBH32_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HCD1514012.1"
                     /translation="METLNSINIPKRKENSHKGDYGKILLIGGSANLGGAIMLAARAC
                     VFSGSGLITVATHPTNHSALHSRCPEAMVIDINDTKMLTKMIEMTDSILIGPGLGVDF
                     KGNNAITFLLQNIQPHQNLIVDGDAITIFSKLKPQLPTCRVIFTPHLKEWERLSGIPI
                     EEQTYERNREAVDRLGATVVLKKHGTEIFFKDEDFKLTIGSPAMATGGMGDTLAGMIT
                     SFVGQFDNLKEAVMSATYTHSFIGENLAKDMYVVPPSRLINEIPYAMKQLES"
     gene            complement(26243..28912)
                     /gene="gyrA"
                     /locus_tag="NBH32_000378"
     CDS             complement(26243..28912)
                     /gene="gyrA"
                     /locus_tag="NBH32_000378"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HCD1514013.1"
                     /translation="MAELPQSRINERNITSEMRESFLDYAMSVIVARALPDVRDGLKP
                     VHRRILYGLNEQGMTPDKSYKKSARIVGDVMGKYHPHGDLSIYEAMVRMAQDFSYRYP
                     LVDGQGNFGSMDGDGAAAMRYTEARMTKITLELLRDINKDTIDFIDNYDGNEREPSVL
                     PARFPNLLANGASGIAVGMATNIPPHNLTELINGVLSLSKNPDISIAELMEDIEGPDF
                     PTAGLILGKSGIRRAYETGRGSIQMRSRAVIEERGGGRQRIVVTEIPFQVNKARMIEK
                     IAELVRDKKIDGITDLRDETSLRTGVRVVIDVRKDANASVILNNLYKQTPLQTSFGVN
                     MIALVNGRPKLINLKEALVHYLEHQKTVVRRRTQYNLRKAKDRAHILEGLRIALDHID
                     EIISTIRESDTDKVAMESLQQRFKLSEKQAQAILDMRLRRLTGLERDKIEAEYNELLN
                     YISELEAILADEEVLLQLVRDELTEIRDRFGDDRRTEIQLGGFEDLEDEDLIPEEQIV
                     ITLSHNNYIKRLPVSTYRAQNRGGRGVQGMNTLEEDFVSQLVTLSTHDHVLFFTNKGR
                     VYKLKGYEVPELSRQSKGIPVVNAIELENDEVISTMIAVKDLESEDNFLVFATKRGVV
                     KRSALSNFSRINRNGKIAISFREDDELIAVRLTSGQEDILIGTSHASLIRFPESTLRP
                     LGRTATGVKGITLREGDEVVGLDVAHANSVDEVLVVTENGYGKRTPVNDYRLSNRGGK
                     GIKTATITERNGNVVCITTVTGEEDLMIVTNAGVIIRLDVADISQNGRAAQGVRLIRL
                     GDDQFVSTVAKVKEDAEDETNEDEQSTSTVSEDGTEQQREAVVNDETPGNAIHTEVID
                     SEENDEDGRIEVRQDFMDRVEEDIQQSSDEDEE"
     gene            complement(28949..30883)
                     /gene="gyrB"
                     /locus_tag="NBH32_000379"
     CDS             complement(28949..30883)
                     /gene="gyrB"
                     /locus_tag="NBH32_000379"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCD1514014.1"
                     /translation="MVTALSDVNNTDNYGAGQIQVLEGLEAVRKRPGMYIGSTSERGL
                     HHLVWEIVDNSIDEALAGYANQIEVVIEKDNWIKVTDNGRGIPVDIQEKMGRPAVEVI
                     LTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSQDLEVYVHRNETIYHQAYKKGVPQF
                     DLKEVGTTDKTGTVIRFKADGEIFTETTVYNYETLQQRIRELAFLNKGIQITLRDERD
                     EENVREDSYHYEGGIKSYVELLNENKEPIHDEPIYIHQSKDDIEVEIAIQYNSGYATN
                     LLTYANNIHTYEGGTHEDGFKRALTRVLNSYGLSSKIMKEEKDRLSGEDTREGMTAII
                     SIKHGDPQFEGQTKTKLGNSEVRQVVDKLFSEHFERFLYENPQVARTVVEKGIMAARA
                     RVAAKKAREVTRRKSALDVASLPGKLADCSSKSPEECEIFLVEGDSAGGSTKSGRDSR
                     TQAILPLRGKILNVEKARLDRILNNNEIRQMITAFGTGIGGDFDLAKARYHKIVIMTD
                     ADVDGAHIRTLLLTFFYRFMRPLIEAGYVYIAQPPLYKLTQGKQKYYVYNDRELDKLK
                     SELNPTPKWSIARYKGLGEMNADQLWETTMNPEHRALLQVKLEDAIEADQTFEMLMGD
                     VVENRRQFIEDNAVYANLDF"
     gene            complement(30893..32005)
                     /gene="recF"
                     /locus_tag="NBH32_000380"
     CDS             complement(30893..32005)
                     /gene="recF"
                     /locus_tag="NBH32_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCD1514015.1"
                     /translation="MKLNTLQLENYRNYDEVTLKCHPDVNILIGENAQGKTNLLESIY
                     TLALAKSHRTSNDKELIRFNADYAKIEGELSYRHGTMPLTMFITKKGKQVKVNHLEQS
                     RLTQYIGHLNVVLFAPEDLNIVKGSPQIRRRFIDMELGQISAVYLNDLAQYQRILKQK
                     NNYLKQLQLGQKKDLTMLEVLNQQFAEYAMKVTDKRAHFIQELESLAKPIHAGITNDK
                     EALSLNYLPSLKFDYAQNEAARLEEIMSILSDNMQREKERGISLFGPHRDDISFDVNG
                     MDAQTYGSQGQQRTTALSIKLAEIELMNIEVGEYPILLLDDVLSELDDSRQTHLLSTI
                     QHKVQTFVTTTSVDGIDHEIMNNAKLYRINQGEIIK"
     gene            complement(32002..32247)
                     /gene="yaaA"
                     /locus_tag="NBH32_000381"
     CDS             complement(32002..32247)
                     /gene="yaaA"
                     /locus_tag="NBH32_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HCD1514016.1"
                     /translation="MIILVQEVVVEGDINLGQFLKTEGIIESGGQAKWFLQDVEVLIN
                     GVRETRRGKKLEHQDRIDIPELPEDAGSFLIIHQGEQ"
     gene            complement(32628..33761)
                     /gene="dnaN"
                     /locus_tag="NBH32_000382"
     CDS             complement(32628..33761)
                     /gene="dnaN"
                     /locus_tag="NBH32_000382"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000969809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCD1514017.1"
                     /translation="MMEFTIKRDYFITQLNDTLKAISPRTTLPILTGIKIDAKEHEVI
                     LTGSDSEISIEITIPKTVDGEDIVNISETGSVVLPGRFFVDIIKKLPGKDVKLSTNEQ
                     FQTLITSGHSEFNLSGLDPDQYPLLPQVSRDDAIQLSVKVLKNVIAQTNFAVSTSETR
                     PVLTGVNWLIQENELICTATDSHRLAVRKLQLEDVSENKNVIIPGKALAELNKIMSDN
                     EEDIDIFFASNQVLFKVGNVNFISRLLEGHYPDTTRLFPENYEIKLSIDNGEFYHAID
                     RASLLAREGGNNVIKLSTGDDVVELSSTSPEIGTVKEEVDANDVEGGNLKISFNSKYM
                     MDALKAIDNDEVEVEFFGTMKPFILKPKGDDSVTQLILPIRTY"
     gene            complement(34039..35400)
                     /gene="dnaA"
                     /locus_tag="NBH32_000383"
     CDS             complement(34039..35400)
                     /gene="dnaA"
                     /locus_tag="NBH32_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCD1514018.1"
                     /translation="MSEKEIWEKVLEIAQEKLSAVSYSTFLKDTELYTIKDGEAIVLS
                     SIPFNANWLNQQYAEIIQAILFDVVGYEVKPHFITTEELANYSNNETATPKETTKPST
                     ETTEDNHVLGREQFNAHNTFDTFVIGPGNRFPHAASLAVAEAPAKAYNPLFIYGGVGL
                     GKTHLMHAIGHHVLDNNPDAKVIYTSSEKFTNEFIKSIRDNEGEAFRERYRNIDVLLI
                     DDIQFIQNKVQTQEEFFYTFNELHQNNKQIVISSDRPPKEIAQLEDRLRSRFEWGLIV
                     DITPPDYETRMAILQKKIEEEKLDIPPEALNYIANQIQSNIRELEGALTRLLAYSQLL
                     GKPITTELTAEALKDIIQAPKSKKITIQDIQKIVGQYYNVRIEDFSAKKRTKSIAYPR
                     QIAMYLSRELTDFSLPKIGEEFGGRDHTTVIHAHEKISKDLKEDPIFKQEVENLEKEI
                     RNV"
     gene            36131..36268
                     /gene="rpmH"
                     /locus_tag="NBH32_000384"
     CDS             36131..36268
                     /gene="rpmH"
                     /locus_tag="NBH32_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCD1514019.1"
                     /translation="MVKRTYQPNKRKHSKVHGFRKRMSTKNGRKVLARRRRKGRKVLS
                     A"
     gene            36389..36742
                     /gene="rnpA"
                     /locus_tag="NBH32_000385"
     CDS             36389..36742
                     /gene="rnpA"
                     /locus_tag="NBH32_000385"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCD1514020.1"
                     /translation="MLLEKAYRIKKNADFQRIYKKGHSVANRQFVVYTCNNKEIDHFR
                     LGISVSKKLGNAVLRNKIKRAIRENFKVHKSHILAKDIIVIARQPAKDMTTLQIQNSL
                     EHVLKIAKVFNKKIK"
     gene            36881..38260
                     /gene="mnmE"
                     /locus_tag="NBH32_000386"
     CDS             36881..38260
                     /gene="mnmE"
                     /locus_tag="NBH32_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCD1514021.1"
                     /translation="MDLDTITSISTPMGEGAIGIVRLSGPQAVEIADILYKGKHLLND
                     VPSHTINYGHIIDPESKEVVEEVMVSVLRAPKTFTREDIIEINCHGGILTINRVLELT
                     MTYGARMAEPGEFTKRAFLNGRIDLSQAEAVMDFIRSKTDRASKVAMNQIEGRLSDLI
                     KKQRQSILEILAQVEVNIDYPEYDDVEDATTEFLLEQSKEIKQEINRLLDTGAQGKIM
                     REGLSTVIVGKPNVGKSSMLNNLIQDNKAIVTEVAGTTRDVLEEYVNVRGVPLRLVDT
                     AGIRETEDIVEKIGVERSRKALSQADLILFVLNNNEALTQEDYTLYEVVKNEDVIVIV
                     NKMDLEQNIDINEVKDMIGDTPLIQTSMLKQEGIDELEIQIRDLFFGGEVQNQDMTYV
                     SNSRHISLLKQARQTIQDAIDAAESGVPMDMVQIDLTRTWEILGEIIGETASDELIDQ
                     LFSQFCLGK"
     gene            38327..40204
                     /gene="mnmG"
                     /locus_tag="NBH32_000387"
     CDS             38327..40204
                     /gene="mnmG"
                     /locus_tag="NBH32_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCD1514022.1"
                     /translation="MVQEYDVIVIGAGHAGVEAGLASARRGAKTLMLTINLDNIAFMP
                     CNPSVGGPAKGIVVREIDALGGQMAKTIDKTHIQMRMLNTGKGPAVRALRAQADKVLY
                     QQEMKRVIEDEENLHIMQGMVDELIIEDNEVKGVRTNIGTEYLSKAVIITTGTFLRGE
                     IILGNMKYSSGPNHQLPSITLSDNLRELGFDIVRFKTGTPPRVNSKTIDYSKTEIQPG
                     DDVGRAFSFETTEYILDQLPCWLTYTNAETHKVIDDNLHLSAMYSGMIKGTGPRYCPS
                     IEDKFVRFNDKPRHQLFLEPEGRNTNEVYVQGLSTSLPEHVQRQMLETIPGLEKADMM
                     RAGYAIEYDAIVPTQLWPTLETKMIKNLYTAGQINGTSGYEEAAGQGLMAGINAAGKV
                     LNTGEKILSRSDAYIGVLIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLRLTDMGY
                     ELGMISEERYARFNEKRQQIDAEIKRLSDIRIKPNEHTQAIIEQHGGSRLKDGILAID
                     LLRRPEMTYDIILEILEEEHQLNADVEEQVEIQTKYEGYINKSLQQVEKVKRMEEKKI
                     PEDLDYSKIDSLATEAREKLSEVKPLNIAQASRISGVNPADISILLIYLEQGKLQRVS
                     D"
     gene            40204..40923
                     /gene="rsmG"
                     /locus_tag="NBH32_000388"
     CDS             40204..40923
                     /gene="rsmG"
                     /locus_tag="NBH32_000388"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCD1514023.1"
                     /translation="MTVEWLAEQLKEHNIELTETQKQQFQTYYRLLVEWNEKMNLTSI
                     TDEHDVYLKHFYDSVAPSFYFDFNQPISICDVGAGAGFPSIPLKIMFPQLKVTIVDSL
                     NKRIQFLNHLASELQLQDVSFIHDRAETFGKGVYRESYDVVTARAVARLSVLSELCLP
                     LVKKGGQFVALKSSKGEEELEEAKFAISVLGGNVTETHTFELPEDAGERQMFIIDKKR
                     QTPKKYPRKPGTPNKTPLLEK"
     gene            40966..41805
                     /gene="noc"
                     /locus_tag="NBH32_000389"
     CDS             40966..41805
                     /gene="noc"
                     /locus_tag="NBH32_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion protein"
                     /protein_id="HCD1514024.1"
                     /translation="MKKPFSKLFGLKNKDDIIGHIEEDRNSNVESIQIERIVPNRYQP
                     RQVFEPNKIKELAESIHEHGLLQPIVVRPIEEDMFEIIAGERRFRAIQSLNLPQADVI
                     IRDMDDEETAVVALIENIQRENLSVVEEAEAYKKLLEIGDTTQSELAKSLGKSQSFIA
                     NKLRLLKLAPKVLLRLREGKITERHARAVLSLSDSEQEALIEQVIAQKLNVKQTEDRV
                     RQKTGPEKVKAQNLRFAQDVTQARDEVGKSIQAIQQTGLHVEHKDKDHEDYYEIKIRI
                     YKR"
BASE COUNT        12920 a         7779 c         6592 g        14592 t
ORIGIN      
        1 aaaatagaaa aaacaagatt cctaaagaat ataggaatct tgtttcagac tgtggacaaa
       61 ctgatttttt atcagttagc ttatttagaa agttttattt aaattacagt ttctattttt
      121 attagatcac aattttattt tagctcttgt tcaagtaatc atttttcgcc aaaaacttta
      181 tactgaatag cttctacatt aaatactttg tcaatgagat catctacacc tttaaattca
      241 gaataatttg catatggatc tataaaataa aattgtggtt ctttaccgga aacattaaat
      301 attcttaata ttaaatattt ctgcttatat tctttcatag caaacatttc atttagcgac
      361 ataaaaaatg gttcctcaat actagaagat gtagatgttt taatttcaat aaatttttct
      421 acagctttat ctgtatttgt tggatcaaaa gctactaaat catagccatg accgtgttga
      481 gagcctggat tatcatttaa aatattccta aactgttctt tcttatcttc gtctatttta
      541 ttatcaatta gctcattaaa gtaatttagc gctaattttt ctccaacttt accggttaat
      601 ttattctctt tatttgattt ttcaatttct gaatcatttt tagtagtctt tgatacacct
      661 tttttatatt ttggaattat tcctttaggt gcttccactt ccttgagtgt cttatctttt
      721 tgtgctgttc taatttcttc aatttcgctg tcttcctgta tttcgtctat gctattgacc
      781 aagctatcat aggatgtttt tgtaactttt gaagctaatt cattaaatag ttctaaaaat
      841 ttctttaaat cctctagcat atcttcttct gtgaatcctt cattcaaatc ataatatttg
      901 aatcttattg atccatgaga atatcctgat ggataatcat tttttaaatc ataagatgaa
      961 tctttatttt ctgcgtaata aaatcttcca gtattaaatt catttgatgt aatatattta
     1021 ttgagttcgg aagataaagt taatgctctt tgttttgcag catttttatc ccgcggaaac
     1081 atatcactta tctttgacca tccttgattc aaagataagt atatgccttc tccttccgga
     1141 tgaaaaagat ataccaaata atatccatcc tttgtttctt ttgttatatt ctcatcatat
     1201 attgaaatcc aaggaacttt actatagttc ccagtagcaa ccttccctac aactgaatat
     1261 ttatcttctt ttatatgcac ttttaactgc ttgggtaact tatcatggac taaagtttta
     1321 tatagatcac ctttatccca atcagatttt ttaactacat tattggtacg tttctcttta
     1381 attaatttaa ggacctgcat aaagttgtct atcatttgaa attccctcct attataaaat
     1441 atattatgtc tcattttctt caatatgtac ttatttatat tttaccgtaa tttactatat
     1501 ttagttgcag aaagaatttt ctcaaagcta gaactttgct tcactataag tattcagtat
     1561 aaagaatatt tcgctattat ttacttgaaa tgaaagactg cggaggctaa ctatgtcaaa
     1621 aatcatgaac ctcattactt atgataagct tcttaaaaac ataacagcaa ttcacataaa
     1681 cctcatatgt tctgatacat tcaaaatccc tttatgaagc ggctgaaaaa accgcatcat
     1741 ttatgatatg cttctccacg cataatctta aatgctctat acacttgctc aattaacaca
     1801 acccgcatca tttgatgtgg gaatgtcatt ttgctgaatg atagtgcgta gttactacgt
     1861 tgtaagacgt ccttgtgcag gccgtttgat ccgccaatga cgaatacaaa gtcgctttgc
     1921 ccttgggtca tgcgttggtt caattcttgg gccaatcctt cggaagatag catctttcct
     1981 tgtatttcta atgtaatgac tgttgattgt ggtttgattt tggctagtat tcgttggcct
     2041 tctttttctt ttacttgctc aatttctttg tcgctcatat tttctggtgc tttttcgtct
     2101 ggaacttcta tgatgtctat cttggtgtat gggcctaaac gtttttcata ttctgctatg
     2161 gcttgcttcc aatatttctc ttttagtttc cctacagcta aaatggtgat tttcatgtcg
     2221 tttggtcctc caaattgtta tcaactttcc agttatccac aagttattaa cttgttcaca
     2281 ctgttccctc ttattatacc aagttttttt acagtttttg atattttcct gacatttatg
     2341 cccggttttt agtgtataac ctgtgtattt gtggataact ctgtgggcat tgtgggtatt
     2401 tctctttaaa ttgtacacaa ttctggataa ctatccccat ttctgtggat aaataccttg
     2461 atgtcttatt acttatcccc gtttttattt cccatattta tcaacggtta tagctttttt
     2521 ccataaaaaa acactaccgt acctcttatt taagaaatac agtagtattg ttttcaatat
     2581 attttagcta gcttttctac gtcgtacagt tgcgattaaa cctatacctg ctactaatac
     2641 aaatatcgct gctgggcttg aactttgatt agttccagtt tttggtaact gtttctcatg
     2701 cccgctacct ttagacgctg acattttagt caattgtttc ccactcttac ttgatacagc
     2761 agttccttct aatgcgcaat cagaaccttc tgcaccgcta ctaacagttc ctctatgcgt
     2821 tggtaacaac acaactttac ctgtcgctgg ttgtttcgct ggattcatca ctttgtcttt
     2881 accaattggt tgtgtatctt taccagactc actacctttg ctaccttgtt gtcctttagc
     2941 tggttgttca cttactgctg gtttacctaa taacatacgt tgtggttctg tcgtatcata
     3001 cttagctaag ttagctgttt ttaaataact tgctagtact tgatctaatg aaataccttc
     3061 ttctctaggg ccaccgaaca tactatatcc gtcgccacct gatgctgtga agtcattcat
     3121 cgttacgtga tatacacgtt ttaaatcaat attttcaaac ttacctgtct ctttatttaa
     3181 aatttgaata gcgttaattc gtttgccaga tggtttattc atatcatagt aaacacggat
     3241 tgaatcagag atatgtagta aaccaccatt cgctgttaac actgtcttac catctttttg
     3301 tgttgttggt gcgcctaaac tatgttcgaa agcagtccag acgtctgaac cttttacatc
     3361 aatttgcgca atcgtatttc caaatggtaa tactgagatt aaatcatagc gtgtcacctt
     3421 acctttggcg atagaggcgc gaatacctcc accatttgtc acggcaaagt cagtcttttt
     3481 agagaaattc ttaacgccat atgcttccat agcatctgta atcgcgtttc ctaaatttgt
     3541 ttcacgcgtt ctaacgtcat ctctttctcc tttgaaatca atggtattgt ttggaataat
     3601 tacctctgca gtttgtgctc taaatgtttg atcagcttga ttaatttgtt cagctaatgc
     3661 tttgttcggt gttacatttt caacgtcttt aacattaatc aatgacggtt taatatttga
     3721 tacctctcca ttgcggtaat taaatgtaac cttaccgata ttcgcaagtg ctgtacctgt
     3781 ttgtgctaat gcatcattgt tataaatttg accattttga agtacggtat gtgaatgacc
     3841 atcaatgact gtaatacgtt tcttcaattg tggattttga cttaattgtt tcactaagta
     3901 atcgccacgc catgtttctt gtgttgaagg atcaatacct aaatgtgata taacaacaaa
     3961 tgtatctacg tctttataaa tacgcatcat ttccgctgtc acactttgta atggatctct
     4021 aaattcaaca cctttaatgc cttcaggtct tgttttcgtc tttgtttctg gtgtcgttac
     4081 gccaataatt ccataacgaa taccattttt cgttacaatc gttgaaggct taaatgcgcg
     4141 ttttccatct ttataaacgt tagtacttaa catcgggaag tctaacatac cctctaactt
     4201 tttcaactgg tcgtatccaa agtcaaattc gtggttaccc actgccatag catcataacc
     4261 tactgcattc atcgctttag ccatttcttc acctttagac tggtttgaaa gtggtaaacc
     4321 ttggaaagcg tctcctgcgt ctaaaattaa atcaggcttt tcttgttctt ttactgtttt
     4381 taatttagcc atacctatga cacgcccttt ttcttcggct agtcggccat ggatatcatt
     4441 tgtatgtaat attttatgtg ttgttgtttg tggtgcatta gcagcaaaca ttcgtggtga
     4501 aagtgatgct gttttagcat ttgataattt gaatgttgct gattgagttg gtttttgtgt
     4561 tgaggcttgt tgtgcatcta catcgtgcgt ttcgtttact gttgttgaaa ctgctgtaga
     4621 tggtttgtta gatggtgcgt tggatgttac tttgttgttt gtatcatcag ctgttccctg
     4681 tgctgatgct gatacgttgg ttgccgcttc ttcaccagat tgatgagccg cttcctttgt
     4741 tggcagtact agttgcctat ctgttgttgc tttgtctatc gttgttactg catgtgcttt
     4801 cattggtgta tgttgctcag ccgcgctcga gacttgccat aatcccattg cactaaaaag
     4861 caaaacgagc catacacttg ttttaagtaa taaagctttc atttcgttcc ctccattaac
     4921 tgtcatacac caatatatta tcataatcct tatattaaaa tgattgattt tatgtaaatt
     4981 atttataaat tacatttaat atcgaattat ttttcacatt tcaactagaa ccttaatttc
     5041 aacaacagcc atttcccacc aacaacataa aaaagcaccc gataaagtcg tctcacagca
     5101 gtgccgaact ttatagggta ctctcattta tattgtatat attggcgttg gaatagcttt
     5161 atccgtatca catagcaata cttctttttc cgtatcaata tcgtgttcgt tcaatacttg
     5221 accaacactc atacgcgcca aatctttcat attattatct tgtgataaat gcgataagta
     5281 aatacgtttc gtgttacctg taatcacgtc tgtcatcgca tgacccgcat cctcattaga
     5341 tacatgaccc atatcgccta aaatacgttg tttcgtcttc catggataac gacacattct
     5401 caacatatcg acgtcatgat tactctcaaa aataaacgca tcgctgccac gtatcatacc
     5461 tttcatacga tcagacacgt atcccgtatc cgttaaaatc gtaaacttct tatagttatt
     5521 atggaaaata taaaattgtg gatctatcgc atcatgtgac acgttaaacg attcaacatc
     5581 gaaacctgca atagattttg tttcataagg attaaaaatg aatttctgat ccatagggat
     5641 gcgactatct ttcttttcaa ttgcctgcca agtcttttca ttcgcataaa ttggcaattg
     5701 atatttacgc gccaaaacac ctaatccttt aatatgatca atatgttcat gggttactaa
     5761 aataccattt aaatcttgaa tattacggtc aatttgacta aacaattctt ccattttctt
     5821 gccagtcaaa ccaacatcaa ctaatagact acctttttca ttttctacaa aagtggcgtt
     5881 acctgtacta ccacttgcta atacactcat gcgtatcaag cggctcatcc tttctattta
     5941 agcagtcaat ctctctatat gactacaata tacatcagta caaatatacg attttcccta
     6001 cagaaactat cagtatatta gctgttttgt atacctttcc tattatgtaa ctttagttca
     6061 tcgaccggtt tcttatattt aaattttgat aatcacgtgc aatgaatttc ttaattatta
     6121 taacaatgat tagacaaata ctaaagtttt taaaatagca gttacactaa ttttacaatg
     6181 cggtactact taaaatatga ataaatgcgc acgttggaga cacagaaatg tcgcctgtca
     6241 gccataataa accgtaccag caaaaagcta gcactgcttc tcacatatga tgagcttgca
     6301 atgctagctt attacgattc atattaatga ttaataattt tagggttatt atttgtcgct
     6361 tcgacatagt aagtattcgt tttgtcctta ccgtcatgct tcactttaat ttcccagttt
     6421 ggttgtaaca attgaacatt cgtttcattc acgactgaat agtaaccaag tctagcatta
     6481 atgacttcat cacctttttt caagtaacga ttataatata atgcctcgat tgcttttctc
     6541 acactaatca cttgatgctt cttatctgca ccatctgtgg gcttaatgtc atccatcatt
     6601 gattgtttat aactagtcgc tttgttatct tctattttaa agttcaacat cgctttgctg
     6661 ttatttaaaa tcggaaaatc atcatacgtt tgttcatatt ttacagaatc tgaactaatc
     6721 tcgcttaatt gatattcagc acctttaaat acgcgcttgt taacgtaatc ttttaaatct
     6781 tttaagttat tatcactaac ttttaccgat tggctgatgt tcgcatttaa taattttcca
     6841 ctatctgatg tggataaatc agaatcacct ttagcttttg aactaaagtc ttttgatcgt
     6901 cccgtaattt gttctaattg tatgccttta acagatttat ttaaaatact ggctggcacc
     6961 ctaatttctt cttgctgaaa attaacttca ttgttactct cgacttcatt aatgtgtgag
     7021 cgattgactt tattaacata aatcgatact aacacgatgt tgacaagaat aaacacgaaa
     7081 atgaaaagtg tctttgtcag tttccagttc atttattcaa gcctcccatc gttataaaca
     7141 taccattcgc catcatattc tacataccaa cgcggtacga gttcactgtt aatctgcact
     7201 tcaatgtgat tatgatctga gttatcctgc atttcgtaac cgatagcgat gtttgttact
     7261 ttttcaaaat taatatcact attgttcgct aagcttgaac gtacagactc taatttcggc
     7321 aacgttttat tatcttcact gtttaaaaca acgtcggtgc gtaataacga acgacgataa
     7381 tcaaagacgc ctttttcacc ccaagtgact tgaatttgat tagagccttc tttattaaac
     7441 gttggataac cgttaaggaa acgttgatat gttaactcgc ctgactgatt attcgtacta
     7501 aacaatctga agtcttcgtt taagaaacca ccatgaccat taataaaatc aaaggttcct
     7561 ggaatcgttt cttccatttt gctggaactt gcttcatctt cggacaagtt tttataatga
     7621 tatttttcat ttttatcgtt atagtttgcg acacctgtat tattgttgta ggttgtaaca
     7681 ccactctttg aactacgaac gatggttgaa tcgtcaaata gtatagcatt cattttctca
     7741 acactaatcg tgttaaatac catgcgatat gtttttaact tttcaggttt acttggtgca
     7801 aaaacatgcg tcgtacgatc aattgtatct ttgtttgtga tgatatctgt gtatggttgc
     7861 atgtcttttt tcactgctgc taatgcatct aaaaaatgat catttttcgt tgtagttgtt
     7921 aattttacgt aatcgtggcg atctttgctt atagcataaa gcacgatatt atcatcagca
     7981 tcatgatcta tgaccaaacg attgaaattg aaatgatttg gtactttcgc attcatgttc
     8041 agtacttggc caagatatgt tgataacggt aaatcatacg taaaatcgaa taatgtaaaa
     8101 tcactgctca aatcaggaat catcaagtta tgatcacgac gaacatgttc cacggacttc
     8161 acttctttat tttttaaagg tttcgtcagc ttattcacat tagaaaccgt cgcaatcgta
     8221 ccttctggat gatcattttt cgaatgaata atctgaaatg gcgtaatagt tgtatccatt
     8281 ttggctgtca taggtgtcgt taaaggtttc gtttcactct tcttactatc tgtattgtcg
     8341 acatttgcaa tatcaggaga aaagttccat accatatatg tcaatacgac actcatcaag
     8401 acaagtagcg ctaaaatgac agatttaata tgctccttat tattcatccc aatcaccgtc
     8461 ttcaatgact tcacatggaa gtgtgataaa gatagatgta ccttgacctt ctacactgtt
     8521 tgcccaaata cgaccattgt gcgcttccac aatctctttc gaaatggcta gtcctaatcc
     8581 agtaccaccc attttacgcg tacgtgcctt atctacacga tagaatcggt cgaatatctt
     8641 atcgacttta ttgataggaa taccgatgcc attatcttta atacgaatcg tcattcgatt
     8701 ataaagtgga ttttgtttca cgtggaactc gacacgttta tcgcctctag aatatttcat
     8761 cgcatttgta atgacattat caaatacttg cgtcatttta tcaggatcaa attctgtgaa
     8821 aatcgtcttt ttcggaatat ctcgaataaa tgttgtatct ttcgcagaca tttcatgtcg
     8881 attaataatt ttattaatga acatgttaaa gtcgataatt tctttgttga tttgatcaga
     8941 ctcattatcc attttagata actgtagcaa gtcattgacc agtcgaatca ttcgttctgt
     9001 ttcttcacgg gtaacagata aaaattgtgg cgcaagttcc tcatctttcc atgcaccttc
     9061 ttcaagtgct tcaatgtaac tattcataga agttaaaggt gtacgtaact catgtgatac
     9121 attagcaaca aattcacgac gctcacgttc aacttgttgt tgttcagtaa cgtcatgtaa
     9181 cacagcgata taaccagtta caaatcctgt ttcctgcaca atcgtactaa agttaacacg
     9241 tgcgattaga ccttcttctt catttaaatc taataagaaa ctatcattat tctcttgaat
     9301 ttcttccagt ttaaattcat cttcaagact taatacactt aacatgtaat atccgatgat
     9361 gtcttctttt gccataccaa gcatcttaag tgccatatca ttgacgatac gaatacgtcc
     9421 acgacggtct gttgcaataa taccatcact catatgggtg ataactgagt ccagtctacg
     9481 tttctcactt tcagtattag cctgcgcttc ttgtacacgt ttagacaagt tattaaatgc
     9541 taaagctaat tcgccaattt catcattacc ataaatcttc acacgttgcg tatagttacc
     9601 tctggacatt tcgaccgtct ggttacgcat atcggtgatt ggtttggtaa tcgttcgagc
     9661 tataaagaat cctaggatga ctgtgattaa taatgaaata gctgtaccaa caatgaatat
     9721 ttgatttata ttatttaatt ggttataaac gtcattaatt tttgattcga tataaatatt
     9781 accaattacc tttttatcga ctttaacggg gatattatat acccagacac ggtccttacc
     9841 accgccataa tcttttaaaa ttaaatgatc gtttgattgt cctagtgata gtgctttttg
     9901 gacagaacta tcattcgctt tttgattgat tagactacgg ttagactgct tcgtcgtcgc
     9961 aataataatt tggtctttat ctataaaacg aatttctcca atttcttgac ggttggcata
    10021 ctcacttaat aaattttgaa tatctttttg tgcatttacg gagccctttt cgtcatatac
    10081 tttttcaata ctaatttcta attgtttagc gtactgcgta atattcttct taaaattatc
    10141 aagcagctct ttttcaagat tatttgtaaa atacagcccg ataatttgca taccaatgat
    10201 aatcagtaat acataaacaa ttacaagttt agtatgaagg gattgtagtt gttttagcca
    10261 cttcattcgt ttcgacctct actcatgttg ttggaggaaa tatccaacgc ctctacgcgt
    10321 cacaatgtat tcaggatgtg acggatcatc ttcaatcttt tcacgtaaac gacgaatcgt
    10381 tacatcgacc gtacgtacat cgccaaagta atcatagccc catactgttt gtaataaatg
    10441 ttcacgtgtc attacttgtc ccatatgttt tgataaataa tggaacaatt caaattcacg
    10501 atgtgttaat tcaatatctt cgccacgttt tttaatagaa tatgcgtctg gataaatcac
    10561 aatatcttta attgtgattt cattcgtcac gttcccagtg tcttgcgctg gttgtgagta
    10621 atgacggcgt aaattcgctt tcacacgtgc aattaattca cgtgtactaa acggttttgt
    10681 tacatagtca tctgcaccta gttctaaacc aagcacttta tcaatttctg agtctttagc
    10741 agttagcata ataattggca tttcgtattt tttgcgcact tcgcgacata cttccatacc
    10801 atcacgacca ggtaacatga tatctaataa tacgatgtct ggttcttctt cataaattaa
    10861 gtcgactgca tcattaccat cgtatgcaca gtaaacatcg tatccttctt tttttaagtt
    10921 aaattctaaa atatcagcaa tcggtttttc atcatcaact acaacaactt ttctagccat
    10981 ttgcataaac ctcttttctt aaatcattaa tattatttta tcattttttg ttctttatcg
    11041 tttatgtttg tgtaaaaaat cacaggatgt cgcttactat ttttgttatt gtgcgcattt
    11101 tccatcttat aacgtatagt aacctattat ataatttacc atttataagc tattaattct
    11161 attttccaat ataaacttat atattaatat tttttattag tttctcacgg cggattgtga
    11221 caatgatatt taacacaatg atgaggtctc tattccaata ataaaattag ggtaatggca
    11281 acttctagtt gcacgtcatt gctcagaaaa taaatatcat tattcattat tcgttacttt
    11341 aacaattgat gttatggagg attggtggca tatcacaacg aagaaatatg ttgacagtag
    11401 catatgtttg tacgtctaat taagatttta ttggctacgt ttatctttca tatccaacca
    11461 ctaagttact tgaggttcat acattataaa gaaaatggac gaacagataa atcaatatga
    11521 ttatcattcg tccatatatc ttttattaat ataatattaa acgcaaaaaa atgcctatcc
    11581 tcttggatag gcatttgtac ggtctcgacg ggaatcgaac ccgcgatctc ctgcgtgaca
    11641 ggcaggcgtg ttaaccgcta cactacgaga cctaaaaaat ggtgactcct acgggactcg
    11701 aacccgtgtt accgccgtga aagggcggtg tcttaaccgc ttgaccaagg agccgttctt
    11761 aagcacaaga tagattatag cacagccaat cttgccatga caagatattt tttacataat
    11821 tttgctatta ttttttgaag aatgttataa gtgaaaaatt gcattttatt ggctgtctat
    11881 ttattataat aattacgcta tttttgaaat ttttctattt taggtgcttt catttacact
    11941 tacgtctact ctattttaat agctattttc gacatcagac ttattacatt caagtatttc
    12001 ttcttaattt cgttaaatat catctaatct tttaaattat gactagcaaa tatcaccttt
    12061 acgttagtaa tttttatcta aataatcgaa tcgatacaga agcaaacata acaaaagcct
    12121 cctaccatta aagatagaag gctcatgtat ttataatcat tgtgtatgac ttatataaag
    12181 ttctaccaca attcttttaa taggtttgtt tgttctctat ctggaccaac tgagaagata
    12241 gaaatttgta cattacataa ttctgaaata cgctctaaat atttacgtgc attttcaggt
    12301 aattcttcta aagtacgcac acttgttacg tcttctgtcc aacctggtaa ctcttcaaag
    12361 attggtttac aacgttttaa ttgatctaag tttgctgggt actcagtaat ttctttaccg
    12421 tctaattcat aagctgtaca gattttcact gtgtctaggc ctgttaaaac atcgattgag
    12481 ttaatagata aatctgtaat accacttaca cgacgagagt gacgtaatac aactgaatca
    12541 aaccaaccta cacgacgtgg acgtcctgtt gttgtaccgt attcacgacc aacctctcta
    12601 atatgatgtc catcttcatc gaataattca gtagggaatg gaccatcacc aacacgtgat
    12661 gtataagctt tacatacacc aattaccttc gaaacgaatg taggacctac acctgtacca
    12721 acagtaacgt tacctgcaat tggattactt gatgtaacga atggatatgt accatggtcg
    12781 atatctaaca ttacaccttg cgcaccttcg aaaagtacct tttcatctgc tacaaatgca
    12841 tcgtctaaga tttttgatgt gtctgttaca aattctttta aacgttgacc tgcagcataa
    12901 tattcttcaa agatatcatc aaatgatgga catgtttcgt taaacatacc tttgaaatat
    12961 gcttgtttat attcaatgtt tgattttaat aatctttcga atgtttcttt ttcaagtaaa
    13021 tctgccatac gaataccgat acgttgaact ttatctacat atgctggacc gatacctttt
    13081 ttagttgtac caatcttatt atcaccacgt aaacgttctt catattcatc ttgtactaag
    13141 tgatatggta gaatcacttg cgcacgatta gatatacgta aattacttgt aggaatgcca
    13201 cgttcattta atccgtctaa ttctttcaat agtgcaactg gatcaacaac gactccgtta
    13261 ccgattaccg ctaatttgtc tttgtaaaag ataccagatg gtactaaatg taatttatat
    13321 gtttctccgc caaattgaat ggtatggcct gcattattac cacctgaaaa acgcgcgata
    13381 acatctgact gttctgccaa gaaatccgtt atttttcctt ttccttcgtc tccccattgt
    13441 gtcccaacta ctacgattga tgacatgtga gcacctccaa gtttttctca tttaaccatt
    13501 tgatattcta ccaatatcga gaccataatg caaataaaaa tcgaacattg attttcggcc
    13561 ttttttaaaa gaaaaccatt ttaacaaatc gtcttcaacg tcctaaaaac cctttaacag
    13621 aggcgttgcc agtactaatt catcactttc aatacacaaa cttaacatat atcaacccgt
    13681 tcagaacaaa ataaaaaccg tacaatttta tcattaaaat gattattgta cggaaaaact
    13741 tttttacatc atatcagcat gtgcataatc gatatctgta aatttattat attgtttcat
    13801 aaagtgtaac ttaactgtgc ctgttggacc attacgttgc ttagcaatga tgatttcaat
    13861 ttcaccgttt tcatcattcg tttgtggctc aaaaccacca tcatcgtcat catcttcatc
    13921 gccgccacgg ttatagtaat catcacggta taagaatgca acgatatcgg catcttgctc
    13981 aatcgaacca gattcacgaa tatcactcat cattggacgt ttatcttgtc gttgttcaac
    14041 accacgagat aactgactca acgcgataac tggacattct aattcacggg ctaatgcttt
    14101 taatgtacga gagatttcag aaacttcctg ttgtctgtta tcagacgcac gtgaaccact
    14161 accttgaatc aactgtaagt agtcaatcac aatcatgtct aagccatgtt cttgctttaa
    14221 tcgacgacat ttagaacgta gatcattaat tcgaataccc ggtgtatcat caataaaaat
    14281 cttcgtacgt gataatttac ctaccgctat agtaaaacga ctccaatctt cctcagtcat
    14341 agtacccgtt cttaagcggt ttgagtcaac attaccagaa ctacaaatca tacgtgtggc
    14401 taactgatca gcacccatct ctagtgagaa aataccaact gtatacatat cttcatgcgt
    14461 tgcaactttt tgtgcaatat taagtgcgaa cgcagtctta cctacagatg gacgcgctgc
    14521 aaggataatt aaatcatttc ggttgaaccc tgctgtcatt tggtctaaat ctcgatatcc
    14581 tgtaggaata cctggtgttt gaccactatt ttgatcaagc tcttcagctg tttcatatac
    14641 ttgtcctaag acgtctcgaa tgtctttaaa gccatcgctt tcacgagaag atgatagctc
    14701 taaaattcga cgttctgcat cacttaaaat cgcatctagt tcaagttcat cattatatcc
    14761 atcattggca atactatctg cagtttgaat caatctacgt tttaatgcat gcttagaaac
    14821 aatgtccgta taatactgaa catttcgtgt cgttggtaca tttgtagata actctgcaag
    14881 atattgcgga ccacccgctt cattcaacgt accttcactt gataactgat ccatcaatgt
    14941 tactacatca atttctttgt tatcttcatt taagtgcatc attgcacgga aaatatgttg
    15001 atgggcaccc ctataaaacg actcaggaag caaaacttcc tgagtagtat taatcaattc
    15061 tggatctata ataattgaac ctaagacaga ctgttcagct tcattgttat gcggcatttg
    15121 attttgctca tacattctat ccatgaatgg ttacacctct tatttcaatc caactttatt
    15181 gttcaactgt gtgtacgcga atcgtacctt caacttcttt atctaattta acaggtacat
    15241 tcgtatatcc tagggaatga attccatttg gtaaatccat tttacgttta tcaattttaa
    15301 tatcatgttg tgcttttagt gcttcggcaa tttgttttgt acttactgac ccaaacaatt
    15361 taccaccttc accagttttt gctgatactt gaacttcaat gtttgataac gtttctttta
    15421 atgctttagc atcttcaatt tcttgttggc gttcttgttt tgcacgtttt ttctgtaact
    15481 ctaattgttt aaggttacct ggtgttgctt ctacagcata attctttttc aataagaagt
    15541 tatttgcata acctactggt acttctttaa cttcaccttt tttaccttta cctttaacat
    15601 cttgtgtaaa aattactttc atgcatcttc actcctactt aattgttctg taattgcttg
    15661 ttgtaattgt gctatcgcct cttcgactgt cacaccttta agttgtgttg ccgcattggt
    15721 taaatgtcca ccgccaccaa gtgcttccat tgttaactgg acatttactg aaccgagtga
    15781 acgtgcagat ataccaatta gattatcttc acgtctcgca acaacatatg atgcttcaat
    15841 accttctaaa cttaacagtt catctgctgc ttgtgcaact gttactggat gataaatttt
    15901 atcgtctgaa ccatgcgcaa tggctatgcc attatcttcg acttttacag ttcgaattaa
    15961 ttcagatcga ttaatataag tatccacatc atcttttaag aaatgttgcg ttagaatcgt
    16021 atctgcacca tgtgcacgta aataactcgc tgcatcgaat gttcttgatc ctgttcgtaa
    16081 tgtaaagttt cttgtatcta caataatacc tgcatacatc actgttgatt caagacgtgt
    16141 taaacgttgt tctgttggtt gatattccag taattctgtt acaagttctg ctgttgaact
    16201 tgcgtatggt tccatatata tcaacaatgg attagagatg aagctttcac cacgtctatg
    16261 atgatcgata acaactttac ggtttgcttt atttaaaaca ttttcatcta aaaccagttc
    16321 cggtttatgc gtatcaacaa tcactacggt tgtcttagat gtcatcatat cccaagcatc
    16381 atctgatgtt ataaatcgtt ctcttaactc tggcttttta tcaatttcgt tcatcacgcg
    16441 tcgtaatgtt ggatcaatgt cagtctcatt taatacgatg tatgcttcta aattattcat
    16501 cattgcaaat ctagacacac cgattgctgc accaattgca tctaagtcag gacgtttatg
    16561 tcccatgata atgactttgt caccctctgc aaggatatct tttaacgcat gtgagatcac
    16621 acgtgctctt acacgagtac gtttctccat cgggtcagtc ttaccgccat agaaacgcac
    16681 attaccatta atacttttaa ttgcaacttg gtcgccaccg cgtcctaatg ctaagtctag
    16741 gcctgattgt gataattcac ctaagtcgat taaattttca gtaccttcac caacaccaat
    16801 acttaatgtt aattgggcac gataaccaac acttttttca cgtaattgac tcaagatatc
    16861 aaatttagat tcttctaagt cagctaatat tttttgattt aaataggcta cgaattgatc
    16921 agaactatac cttttaaaga atatattata ctcagttgcc catcgactaa taacacgcgt
    16981 taccattgaa ttgatttccg aacgctgcgt atcattcata ttttgagtaa tctcatcgta
    17041 gttatctaaa aataatgtcg caatgattgg tttagaattt tcatatagtt catttgtttg
    17101 tacttgttca gttatatcaa agaaatagag gcagtgatca ttctcagaat aacgtacttg
    17161 gaaatgatac tgattatatt ctatttcaac ggatttcact ctatctaatt gctttaaaat
    17221 gtttggaaat acttcattta cagattcaga aatgacattt gcttccatat gatctgtcat
    17281 aaattggtta acccattcga tgtgatcatt ttcatctaaa acaatgatac caattggtaa
    17341 atgtttgatt gctttattat ttgttgttga aatttgagca ctcaaaccat ctacataact
    17401 atccattttc attaaagctt gtctgaataa aacgatgcta acaataatca tcacgacaag
    17461 aacgatagat gcaattagtg ctataagact attaaagata aaccatacac ccattaaaac
    17521 aattgctgtg atgatcatga tgacaaatgg tattagtaaa gctttcttag tggactgccg
    17581 attcattatt ccacctctat tcacttttta gaattatttt tcatgattcg cttcaaattc
    17641 aaacttaaat cgataacacc aagtagtcct acaatatgtg tcgtaggtgt cagtattgta
    17701 ccgataatca atagtaaaat cgttattgca ttcggcaaac ctttcgcttt accaaagaaa
    17761 tgaataacac ttaaaccttg aatatacatt actaatgata acacaagttg gaagtttaaa
    17821 agaatgctct ggaacacact cggttgacct gtaaataata aacatatgat aacaataatg
    17881 tatatccata ataaaatacc gctcatttgc cacgcgaaaa gtggcttaaa tacaggtgta
    17941 gcgattttaa attttcgtaa aatcggaaat gtaacgatta agttaattaa gacgattaaa
    18001 aatgtaatga taatgatgaa acctggtaat tgaacggtcg cttgtctaaa cccttcttct
    18061 aatatttggg tcatattcgc atcggcaccg ctcatcgtaa tcgcttcatg taatgtttgc
    18121 ttgaaaggtt ttactatgct cgctgatggt ggaattcttc cgaatgtttg tagtaacata
    18181 aaagcgatta atgaaattaa gctcatcgct actgttgtta cgtataatat tctttcttta
    18241 gacgttcttt ctttgagcaa ttgaccaata attaaacttg caattaagac taatatgatg
    18301 gcacttaaaa cgaaagtatt acctaaaaca gttgttataa ttactgtaat aagtgcacta
    18361 atcccgaaag attgtattga tttattccat aaaacgatac ctggtatggt tgcaaataat
    18421 gcaaatatta gtcctaaacc aggcactaga tataaagcta aagcgacaaa taccaacgca
    18481 atcgttgcaa ttattgttgc tttaggttgt atttttgaaa acacataagc cactcccata
    18541 tttttaacta tagctattat tttaacctct ttaatggaat ttaacaattt atagattgta
    18601 tgcttctatt tcatttaatt gaataataac tttcatgttt tataagtaat taacatactc
    18661 atttgaatcg cttttgtgtg ctttcatttt caacatgatt atttaatccc actacatagc
    18721 aatcaagctt gatttagatt tacaattcat ttccactctc atgtactcta gatgtctttg
    18781 aatatgataa ctgtgattta gtggcttcat tttttgaaaa tatatacata ttattactta
    18841 cgcttaaaat gctttaaatt taagaaatga tataagttag gtgcccaggt actaaaattt
    18901 agtaggaatc catcatgccc aacattatca ggcacgaaga aatgacgatg atatttaaaa
    18961 cgttcaccta atgcacgaac ttgatcatcc ggatatagca aatcatctat gaaccccatc
    19021 gttaacactt ttgtttctaa atttttaaaa acatgcgtta cgtctgtgcg acctcggtca
    19081 atgttgtgac tatccaatac atctagcagt gtcagataac aattcaaatc aaaatgttct
    19141 ttaaatttat taccttgatg ttgttggtat gcgactactt catccggcgt gaaacgttca
    19201 tcataacttt ttgatgaacg atatgtcaaa aaacctaatt ggcgtgcaat acttagacct
    19261 tccttaccac caagatgaat ggcttgcctt gcaatttcat tgaaagctct actataagat
    19321 gatgttcgac ttgttgcagc aaggataatg gctttatcta cttcaaactg ttgattgtaa
    19381 agtagttcca ttgcttgcat acctccaaga cttcccccta ttaaaatatt aatcttatca
    19441 taaccaaggg cttgtatacc tcgttcattc gctctgacta tatctcttaa tgttaatttt
    19501 ttaggaaaat gagggtcgtt taaaggtgaa cttgaaccga aaggactacc aataacatca
    19561 aatgttaaaa attgataatc gtgaatgggt atatatcccc catcaataat ttctcgccac
    19621 caacccggat aatcatctgt tccatatgtt aaatgattgc cagttaatgc atgacaaact
    19681 acaactaatg gttgtccatg ataaccgaca tgctcatatc tcaaacgcaa gttatctatg
    19741 acttccccag attctgtaat aaatttccct aaatttaaag tatctactgt gtaatttgtc
    19801 attgttcttt cctccttaaa caaaaaaaac ttctcaccct attgaaaagt aagaagtctt
    19861 tatacttatc attcgagtaa ctcgttggtt ttagcaccgt gctataaagt cggttgctga
    19921 agtatcacag ggccaagtcc ctcaacttct cacaataaga aatatgaaat tgttatgtgt
    19981 tagttgagat tcagtgatga attactttta tcatttaaaa tgttgttatc attgtcatgc
    20041 gttagccaaa tcgcttacgt atacacgatt cccaatctta acatagacga tttgtatatc
    20101 agaattttct gattactaac agtttaccta agtttaaata tctgttcaat gattttcagt
    20161 tatttttaaa agaaaaatcg taatgctgcc atgataacaa tcccactaat aattgtaata
    20221 gttaaactac gcgtgattat agataaaata accgtcggaa tgagcgcgat aatgtaaggg
    20281 atgtttaatg tatacccctc accatgaggc gtctgttgaa taatgctgtc tatgacaagt
    20341 gccgtaaata gtgtgattgg gataaatgat agccatcgaa ccacgacatc aggcaattgc
    20401 acttttgaaa tcatgataaa aggtataatt cgaattaata gcgttacgat accacacaat
    20461 aaaataagta ttaacatgtt catatgagtt atcatcgttc catcatcact cctaacgctg
    20521 ctgaaattgt ggctgcaatt aatattgcta gatatgaagg cataaacata cttagcgata
    20581 acatcattac tatgacggca ataatgagta ctatgtaaat tcttaatcgc gatttagtaa
    20641 ttgattcaaa ttgcgcaatg gccaaaaaga taaacatagc cgtgatagca aaatctaacc
    20701 ctagcgtttg cggatttgag atatattcgc caaataaagc cccagctaca catgaaattg
    20761 cccaaaataa atatgctgtg atattaagac catgcatcca acgatcattg atagcttctc
    20821 cttttaaata aggtgtaatg gcgacgccaa acgtttcgtc agttactaat gaacctaatc
    20881 caacacggtt ccaaaaccca tatgtcttga agtttggtgc aagcgacata cttaaaagga
    20941 acattcttga atttacgata aatacagtta gtacaatcgc tgatatcggt gtacctgcta
    21001 taaacaacgc gcacataata aattgcgcag caccggcata tataacaaga cataacaaga
    21061 taatttctaa aatactaaag ttttgagacg aagccacaat accaaatgaa ataccaacac
    21121 cggcataacc caataatgtt gggatacact cttgtacgcc ttgtctaaaa cttaaatgtg
    21181 ttgccatctc aattacctcc tttgccactt tatatttata taattacgaa aaaataatat
    21241 acctagtcta atacagttct ataccctaag caataacatt agactccagt ttgtctgagg
    21301 ttagacatac tggagtcttc aatcagcttt atatcaactt tttatttttg agaattttca
    21361 tttttttatt taagactacc tccatatttt ctacggattt gtagttgttt ttaagtacta
    21421 attttataat tttttatatc taatgatgag tctgggatat tgattcatgt aacactccct
    21481 tataatcatc atcctctccc cctaccctac tccatcgata ttactcatac tacatcaacg
    21541 aaatcagttt tttatcactt aatttcctat aatagtgatg ctcaaaattg ttacgtttta
    21601 gattgtttta gttcatcata attatcccgt attgttgcta taatgaaatg cgttcacccc
    21661 attaaaccac aaacttaatt tattgttgtt atgtgcattg gctcactatt atacttttac
    21721 agcacaaaaa aagtggcgac agcttcgtca ccacttttta aaatattatt taaagtatct
    21781 tgcccttgct ttaagtatac gtagatatat actttttaaa gcttgtagct aaagccttta
    21841 tttaactggt tttgaaattt gtgttttacc acccataaat ggtactaatg cttctggaat
    21901 tgttactgtt ccatcttcat tttggtaatt ttcaacaata gcagcaaatg tacgtccaac
    21961 tgctaaacca ctaccattta atgtatgtgc taattctggt ttagctgctt tatcacgctt
    22021 gaagcggatg ttagcacgac gcgcttggaa atccgtacag tttgagcatg aactaatttc
    22081 tttataatca ttgtagcttg gtaaccaaac ttctaaatca tatgttttgc ttgcactaaa
    22141 tccaatatca cctgtacata aaataacacg acggtatggt aaacctaact cttctagaat
    22201 tgcttctgcg tttgttgtca tttcttctaa agcattccat gaatcttcag gttgttcaaa
    22261 acgtaccatt tccactttat cgaattgatg taaacgaatt aatcctcttg tatctctacc
    22321 tgctgatcct gcttcactac ggaaacatgc agattgacca gtgaattttt caggaagtac
    22381 acctggttga ataatttcat tacggtagaa attcgttaat ggtacttcag cagttggaat
    22441 tgtatataat ccttcttttt ctactttaaa taaatcttct tcaaatttag gtaattgacc
    22501 tgtaccatac attgtatctg cgttcacaag ctgtggtacc atcatttctg tataaccatg
    22561 ttgtgttgta tgttttgtaa tcatatagtt cattaaagca cgctctaatt gcgcaccttc
    22621 atttgttaaa tatacaaaac gcgcacctga aacttttgct gcacgatcaa aatcagccat
    22681 tttcaattct tctacaatat cccaatgtgc tttgggttca aatgaaaact cacgtggtgt
    22741 accccacttt ttaacttcaa cgttatcttc atcagattca ccttgaggta catcatcact
    22801 tattaaattt ggaatacgac aaaggatacc tgtcatttta ttatcaattt catttaattg
    22861 actatctttt tctttaatat cgtcacctaa tgtgcgcatt tcagcaatca catcatcagc
    22921 attttcttta ttacgttttt ttaatgcgat ttcttcgctt actttattac gacgtgcttt
    22981 catttcttct gttgcactaa ttaatttacg tcgttgctca tccaattcta aaatttcatc
    23041 tacaactttt ggatcatctc cacgtaattc aattttgctc ttaactgtgt caggctcatt
    23101 tctgaataat ctaatgtcta acattaacct tcatcctttc ccaaataatt atcatttatt
    23161 atggaatgac gtacgtcttt attttttaga aaataaaaaa agaccacatc cctacaaggg
    23221 acgtggtcta cgcgttgcca ccctatttaa caatttaagt tataaagata cactaaacct
    23281 aaattgcact tcactaaaat aacggttatc accgattgtt cttttaaatt aagtaggtag
    23341 attcatatat atgttgattc ttgttcacac taaccacaag ctctctgata tcgaacacta
    23401 tatattactt gtcctacgaa caatgtctta ttaagttatt tttaatatag caaactatat
    23461 ttgctttttc aagtaacgat ttcaaacatc actcatgtcg atttagtgac atgcagtcgt
    23521 ttgataaatt gattgcttta aatactgtgc aaccgcttca atatctttat gaaattgacg
    23581 gtcatgtgta atggatggca cgatacttcg aaactcctca tatttacgac gtgtttttgg
    23641 tgataaacct tcaacacctt ttaactctgc tgcttgtaat gcaataacac attcgattgc
    23701 cagcacacgt cttgcatttt caataatttg ataaccatgt ctagcagctg tagttcccat
    23761 agatacgtga tcttcttggt tcgcagatga agtgatagaa tcaacactcg ctggatgcgc
    23821 taaagtttta ttttcagaaa cgagacttgc agcagcatat tgcataatca tcgcgccact
    23881 ttgcaatcct ggctctggac taagaaatgc tggtaaatca ccatttaatt gaggatttac
    23941 tagtcgctct agacgacgtt ccgatacgtt tgctaattca cttacaccta atttaagatg
    24001 atctaatgca aaagcaatag gttgtccatg gaagttacca cctgaaataa caaacgtttc
    24061 atttgcttcc tcaaatataa gtggattatc attagccgca ttcatttcaa attctaattg
    24121 ctgtttaaca taattgaata cttgaaaact cgcgccatgg atttgtggta tacaacgcaa
    24181 cgtatatgca tcttgtacac gtatttctgc ttgtcgcgtt gttaatgctg atccttctaa
    24241 ccaatcacgc atacgcgctg ccacattaat ctgttcttga aaattacgaa cagcgtgcac
    24301 atcatgtcga tatgcatcta taatgccatt aagagactga tgcgttaatg cagcaatcca
    24361 ttcagattgg taacctaaat cttctgcttc tatataacta atgacacctt gagctgtcat
    24421 agcttgcgta ccattaatca atgctaaacc ttctttagcc tgaaggttca aaggttgtct
    24481 atttaattct cttaatacat cgtcactatc cttttcttcc cctctgtaca atactttccc
    24541 ttcaccaatt aatgctaatg ctaagtgtga taatggcgct aaatctcctg atgcaccgag
    24601 agagccttgc tgtgggatta tcggtataat acgttcattt ataaaaaatt gtaattgtct
    24661 cactaattct aaagtggcac ctgaatgacc ttttaataat gtattcaatc gtaaaatcat
    24721 catgactaat gctacttctt ttgaaaatgg ctcacctagt ccacaggcat gtgagcgtat
    24781 cagattcact tgtaattcat tatattgcgt cgggtctata cgtacatcac taaataaccc
    24841 aaaacctgta gtaataccgt aaaccgtttc ctcattttca ataatacgtt caactaccgc
    24901 tctacttttt ttgacacgtt ctaacgcatc atcaataatt tcaatctttg attgttgttg
    24961 taaaaatgat ttaatatcct caattgttag tgtctcacca tctaaatata aagtcatata
    25021 tgttaccccc ttgtttatat taagtaaccc atccttcttg aagtatacgt tttcattttt
    25081 attgaaacaa tggttttacg tacatttata acctattatc agagcactat tgtagtgcgt
    25141 taaaggatat taagattgtt gtaagcatat ttaataattt atctattgac gaattgcata
    25201 tacaggtata gtattttcta ttgtatttaa cgacaaataa taatgaattc agaaatttat
    25261 aatacatttt gttaaaagtt actatatatt tttaaaattg aataaattcg gaaaaggctt
    25321 ttacatggga ggttatatca ctatggaaac gttaaattct attaacattc ctaagcgtaa
    25381 agaaaattca cataaaggtg attatggcaa aattttatta attggtggat ctgctaactt
    25441 aggtggtgcc attatgttag cggctcgtgc atgtgtattt agcggtagtg gtttaatcac
    25501 tgtagctaca catccaacaa atcattcagc attacattct cgttgcccag aagcgatggt
    25561 tattgatatt aatgatacga aaatgttgac gaaaatgatt gaaatgactg acagtatact
    25621 aattggtcca ggtcttggcg ttgatttcaa aggaaataat gccattacat tcctactaca
    25681 aaatatacaa ccgcatcaaa atttaatcgt agacggcgat gcgattacaa tctttagtaa
    25741 actgaaaccg caattaccta catgtcgcgt gatttttact ccgcatctca aagaatggga
    25801 gcgattaagt ggtattccta ttgaggaaca gacatatgag cgtaatcgtg aagcagttga
    25861 tcgtttaggc gcaactgttg tacttaaaaa acatggtact gaaattttct ttaaagatga
    25921 agactttaaa ttgacaatcg gtagtccagc aatggcgact ggtggtatgg gcgatacact
    25981 tgctggtatg attacaagtt ttgtcggtca atttgataac ttaaaagaag cggttatgag
    26041 tgccacatat acacatagtt ttattggcga aaaccttgca aaagatatgt atgtggtacc
    26101 accatcaaga cttatcaatg aaatacctta cgcaatgaaa caattagaaa gttagtcatt
    26161 actaatcatt gaatatagta aagcattact ttctagcaca aaaataagac ttccctacat
    26221 atagggaagt cttatttttt tattattctt catcttcatc tgatgattgt tgtatatctt
    26281 cttcaacacg atccatgaaa tcttgtctta cttcaatacg tccatcttca tcattttctt
    26341 ctgaatcaat cacttcagta tgaattgcat ttcctggtgt ttcatcattt acaaccgctt
    26401 cacgttgttg ttcagtacca tcttcagata cagttgaagt agattgctca tcttcattcg
    26461 tttcatcttc tgcatcttct tttactttag caaccgttga aacaaattga tcatcaccta
    26521 agcgaattaa gcgaacacct tgtgctgcac gaccattttg agaaatatct gcaacatcta
    26581 gtcgaataat gacacctgca ttagtaacaa tcattaaatc ttcttcacca gttactgtag
    26641 tgatacatac aacattacca ttacgctcag taatcgtagc tgttttaata cctttaccac
    26701 cacgatttga taaacgatag tcattaactg gcgtacgttt accataacca ttttcagtaa
    26761 ctactaatac ttcatcaaca ctgtttgcat gagctacatc aagccctaca acttcgtcac
    26821 cttcacgaag tgtaatacct ttcacacccg ttgctgtacg gcctaaagga cgtaatgttg
    26881 attcagggaa tcgaattaat gatgcatgtg atgtaccaat caagatatct tcttgaccac
    26941 ttgttaagcg aactgcaatt aactcatcat cttctctgaa cgaaatcgca atcttaccat
    27001 ttctatttat tcttgagaag ttacttaatg ctgaacgttt aacgacacca cgtttagttg
    27061 caaacactaa gaagttgtct tcactttcaa ggtctttaac agcaatcatt gtactaatga
    27121 cttcatcatt ttcaagttca atagcattca ctacaggaat acctttagac tgtcttgata
    27181 actcaggcac ttcataacct ttaagtttgt atacacgacc tttgttagta aagaacaata
    27241 catggtcatg tgtacttaaa gttaccaatt gactgacaaa atcttcttcc aatgtgttca
    27301 taccttgaac accacgacca ccacggtttt gagcacgata tgtagatacc ggcaaacgtt
    27361 taatgtagtt attatggctc aaagtaatta ctatttgttc ttctggaatt aagtcttcat
    27421 cttctaagtc ttcaaatcca cctaattgaa tttctgtacg acgatcatca ccgaaacgat
    27481 ctctaatttc agtcaattca tctctaacta actgtaataa cacttcttca tcagctaaga
    27541 ttgcttctaa ttcactaata taatttaata actcattata ttcagcttca attttgtctc
    27601 tctctaaacc tgttagacgt cttaaacgca tgtctaaaat agcttgagct tgtttttcag
    27661 aaagtttgaa gcgttgttgc aagctttcca ttgcaacttt atctgtatct gactcacgaa
    27721 tcgttgaaat aatttcatcg atatggtcaa gtgcgatacg taatccttct aaaatgtggg
    27781 cacgatcttt agctttacgt aagttgtatt gcgtacgtct tctaacaact gtcttttgat
    27841 gctctaaata atgtaccaac gcttctttta aattaataag cttcggtcta ccatttacaa
    27901 gtgcaatcat attcacacca aatgatgttt gaagaggtgt ttgtttgtat aagttattta
    27961 aaatgacact agcatttgca tccttacgca catcaataac gacacgcaca ccagtacgta
    28021 aacttgtttc atcacgtaaa tcagtgatac cgtcaatttt cttgtcacga acgagctctg
    28081 caattttttc aatcatacga gccttattca cttggaaagg aatttcagtg acaacaatac
    28141 gttgacgtcc gcctccacgt tcttcaataa ctgcacgaga acgcatttga attgaaccac
    28201 gacctgtttc atatgcacgt ctaataccac tcttacctaa aataagtcca gcagttggga
    28261 aatcaggacc ttcaatatcc tccattaact cagcaattga aatatcaggg tttttactta
    28321 agctaagtac accattgatt aattctgtta agttatgtgg tggaatattc gttgccatac
    28381 ctaccgcgat acctgatgca ccattggcta acaagttagg gaatcgagca ggtaagactg
    28441 acggctctct ttcattacca tcatagttat cgataaaatc tattgtatct ttattaatat
    28501 ctcgtaacag ttcaagtgtg attttagtca tacgtgcttc agtataacgc attgctgctg
    28561 cgccatctcc atccattgaa ccaaagttac cttggccatc aacaagcgga taacgataac
    28621 tgaaatcttg agccatacgt accattgctt cataaataga taagtcacca tgagggtgat
    28681 atttacccat tacgtcacca acgatacgtg ctgatttttt atatgattta tccggtgtca
    28741 taccttgttc atttaatcca tatagtatac gacgatgtac tggttttaaa ccgtcacgaa
    28801 catctggcaa tgcacgagca acgataacac tcatcgcata atctaaaaat gattcacgca
    28861 tttcactggt aatatttcgt tcatttattc ttgattgagg taattcagcc atcaagagtt
    28921 cctccttcaa aagttcagtt cacagcgctt agaagtctaa gtttgcataa actgcattat
    28981 cttctataaa ttgtctacgg ttttctacaa catcacccat taacatttca aatgtttggt
    29041 ccgcttcaat cgcatcttca agttttactt gtaaaagagc gcggtgctca gggttcattg
    29101 ttgtttccca taattgatct gcattcattt ctccaagacc tttgtatcgt gcaatagacc
    29161 attttggtgt tggattcaat tcagatttaa gtttatcaag ttccctatca ttgtatacat
    29221 aatacttttg tttaccttgt gtcagtttat acaacggtgg ctgtgcaata tacacatagc
    29281 ctgcttcaat taacggtctc ataaatcgat agaagaatgt taataacaat gttctaatat
    29341 gcgctccatc cacatcggca tcagtcataa tgacgatttt gtgatatctt gctttcgcta
    29401 gatcaaagtc gccaccgatt cctgtaccaa atgctgtaat catttgacga atttcattgt
    29461 tattcaaaat tctatctaat cgtgcttttt caacatttaa tatcttacct cgtaatggta
    29521 aaatcgcctg tgttctagag tcacgaccag attttgtaga ccccccggca gagtcccctt
    29581 cgactaagaa aatctcacat tcttcaggac ttttactaga gcaatcggct aatttacctg
    29641 gaagacttgc tacatctaac gctgatttac gacgtgtaac ttcacgcgct tttttcgcag
    29701 caacacgtgc acgtgccgcc ataatacctt tttcaaccac tgtacgtgcg acttgtggat
    29761 tttcatataa aaatcgttca aagtgctctg agaataattt atctacaact tgacgcactt
    29821 cagaattacc taattttgtc ttcgtttgac cttcgaattg aggatcacca tgtttgatag
    29881 atataattgc tgtcatgcct tcacgtgtat cttcaccaga aagtctatct ttttcttctt
    29941 tcataatctt gctacttaaa ccataactat ttaagacacg cgttaatgca cgtttgaatc
    30001 cgtcttcatg cgtaccacct tcgtacgtat gaatgttatt tgcgtaagtt aaaagatttg
    30061 tggcatatcc tgagttatat tgaatcgcaa tttctacttc aatatcatct ttagattgat
    30121 gaatataaat tggctcatca tgaataggtt ctttattttc gttcaataac tcaacataag
    30181 atttaatacc gccctcataa tgataggagt cttctctaac gttttcttca tcacgttcat
    30241 ctcttaatgt gatttgaatt cctttgttta agaaagcaag ctctctaata cgttgctgta
    30301 atgtttcata gttgtagaca gttgtctctg tgaagatttc tccatctgct ttaaaacgaa
    30361 tgacagtacc tgtcttatca gttgtgccaa cttcttttaa gtcaaattga ggtacacctt
    30421 ttttatatgc ttgatgatat atagtctcat ttctgtgtac atatacttct aagtcttgtg
    30481 acaatgcgtt tacaactgat gaaccaacac catgtaaacc accagatact ttgtatccgc
    30541 caccgccgaa tttaccacca gcatgtaaaa cagttaaaat aacttcgaca gctggacgtc
    30601 ccattttttc ttgaatatca actgggatac cacgtccgtt atccgttact ttaatccagt
    30661 tatctttttc aataacaact tcaatttgat ttgcataacc agctaatgct tcatcgatac
    30721 tattatcgac aatttcccac actaaatggt gcaaacctct ctctgaagtc gatcctatat
    30781 acatacctgg tcttttacgt actgcttcta aaccttctaa tacttgtatt tgcccagcac
    30841 cataattatc cgtgttgttt acatctgaca atgcagtcac catcgctttc tgttacttta
    30901 taatttcacc ttgattaata cgatacaatt tagcgttatt catgatttca tgatcaatac
    30961 catctacaga tgtcgtagtg acaaatgttt gtactttatg ctgaatcgta cttaataaat
    31021 gcgtttgacg cgaatcatct aattcactga gtacatcgtc taataataag atgggatatt
    31081 ccccaacttc gatattcatt aactcaattt cagctaattt aatggacaaa gccgttgtac
    31141 gttgctgtcc ttgagaacca tatgtttgag catccatgcc attcacatca aaacttatat
    31201 catctcgatg tggtccgaat aagctaatgc ctcgttcttt ttctctttgc atattatcgc
    31261 taagaataga cataatttct tcaagtcgtg ccgcttcatt ttgagcataa tcaaatttaa
    31321 gactaggtaa ataatttagc gacaacgctt ctttatcatt tgtgatacca gcatgaatcg
    31381 gtttagctaa cgactctagc tcttgaataa aatgtgcacg tttatcagtt actttcattg
    31441 catattcagc aaactgctga tttaatactt ccaacattgt taagtccttt ttttggccta
    31501 attgtaactg ctttaagtaa ttattctttt gctttaaaat acgttggtat tgagctaaat
    31561 catttaagta aacagcagaa atttggccca actccatatc tataaagcgt cgtcttattt
    31621 gaggagagcc ttttacaata ttcaaatctt ctggcgcaaa tagaaccaca ttgaggtgtc
    31681 caatatattg agttagtcga ctttgctcta agtgattcac tttgacttgt ttaccttttt
    31741 tagttataaa cattgttaat ggcatcgtgc cgtgtctata actaagctca ccttctattt
    31801 tagcataatc agcattaaaa cgtatgagtt ccttatcatt actcgttcta tgactttttg
    31861 ctaaagctaa ggtataaatt gattcaagta aatttgtctt tccttgtgca ttttctccaa
    31921 tgaggatatt cacgtcagga tgacatttca acgtaacctc atcatagtta cgataatttt
    31981 ctaattggag tgtatttaac ttcattgttc accttgatga atgattaaga aagaaccagc
    32041 atcttcaggt aattctggga tatctatacg atcttgatgt tctaactttt taccgcgacg
    32101 tgtttcacgc actccattaa ttaatacttc aacgtcttgc aagaaccatt ttgcttgacc
    32161 accagattca ataatccctt ctgtttttag aaattgacct aaattaatgt ctccttctac
    32221 tacaacttct tgaaccaaaa taatcactcc atttctttgt atgtcattaa tatattatac
    32281 ctttttttgt cacaaatttc atttaaaata gaggtagaaa ccgtttacat ataaataatt
    32341 aattcttatt tttgggcaat actgtcattt tcaatttttt aacgctattt aacggcgttt
    32401 aacttttata ttcaacttgt tattgactat taaaatttta aatttaaagg gcatataagc
    32461 acacttatta aattttggat acatttgtca taaatttaat tggcgtcttt tatttgctgt
    32521 ttaaaatgct gtcaaattta ttttacatac gcatctagaa ttatttttgc caaaaaataa
    32581 aggatgacgt tttaattaat cacgtcatcc tttatttata tttattttta gtaagttctg
    32641 attggtaaaa ttaattgcgt tactgagtcg tcaccttttg gttttagaat aaatggtttc
    32701 attgtaccga agaattcaac ttcaacctca tcattatcga ttgcttttaa agcatccatc
    32761 atatatttag agttgaatga aattttcagg ttaccacctt caacatcgtt tgcatcgact
    32821 tcttctttta cagtaccaat ttctggtgat gtagaagaca attcaacaac gtcatcacct
    32881 gtacttaatt taataacgtt attaccacct tcacgtgcta ataaagatgc acgatcaatc
    32941 gcatgataaa actccccatt gtctatactt aatttaattt catagttttc agggaataaa
    33001 cgtgttgtat caggataatg tccttctaat aatcgagaaa taaagttcac atttccaact
    33061 ttaaataaaa cttggtttga agcaaagaag atatcaatgt cttcttcatt gtcagacata
    33121 attttattta attcagctaa agccttacct ggaatgatga catttttgtt ttcagaaaca
    33181 tcttctaact gcaactttct tacagccaag cggtgcgagt cagtcgctgt gcatattaat
    33241 tcattttctt gtataagcca gttcacacca gttagtactg ggcgtgtttc tgaggtggac
    33301 actgcaaaat tcgtttgtgc aattacgttt ttaagcactt ttaccgacaa ttgaattgcg
    33361 tcatctctag aaacttgagg taataaagga tattgatctg gatctaagcc actcaaatta
    33421 aattcagaat gacctgatgt aattaatgtc tggaattgtt catttgtaga taatttaaca
    33481 tctttaccag gtaatttttt tataatatca acaaagaatc gtccaggaag tactactgag
    33541 cctgtttctg aaatattgac aatatcttcg ccatctacag ttttaggaat agtgatttct
    33601 attgaaattt cagagtctga accagttagt ataacttcat gttctttcgc atcgattttg
    33661 ataccagtta atataggtaa tgttgttctt ggtgaaatag cttttaatgt gtcatttaat
    33721 tgtgtaataa aataatctct tttaatagtg aattccatca ttaattaaaa ctccttccag
    33781 tcgtttatat ataattatat aggttttaag ttcttagtaa tagtagtagg tgctgtggat
    33841 tggtggataa gtcttaaaaa catagatttg gcgaagttat acacatgttg ataacttgtg
    33901 tatgatgtgt atgactttta aacattatcc acaaaatgag ttttgctcat cttgccacat
    33961 tagtatatag aaatgtaaaa aatgacagtt attattagtg ctgttatata acagattaca
    34021 tttcccaaag tttcctactt atacatttct tatttctttt tcaagattct ctacttcttg
    34081 tttaaaaata ggatcttctt ttaaatcttt agatattttt tcatgagcat gaatgacggt
    34141 cgtatgatca cgcccaccaa attcttcacc aattttaggt aatgagaaat ctgtaagctc
    34201 tctagacaag tacatagcta tttgacgcgg atatgcaatt gactttgtac gtttttttgc
    34261 actgaaatct tcaattctaa cattatagta ctggcctaca attttttgaa tatcttggat
    34321 ggtaatcttt ttagattttg gtgcttgaat gatatctttt aaagcttcag cagttaattc
    34381 agttgtaatt ggttttccta ataattgtga atatgcaagt aaacgtgtta atgcaccttc
    34441 taattcacga atattagatt gaatttgatt tgctatataa tttaaagctt ctggtggaat
    34501 atctaatttt tcttcttcaa ttttcttctg caaaattgcc attcgagttt cataatctgg
    34561 tggcgtaata tcaacaatta gcccccattc aaagcgtgaa cgtaatcgat cttctaattg
    34621 tgcaatttcc tttggtggtc gatcactcga aataactatt tgcttgttat tctgatgcaa
    34681 ttcattaaaa gtatagaaaa attcttcttg tgtttgtact ttattttgta tgaactgaat
    34741 atcatcgatt aataagacgt cgatattacg atatctttct ctgaaagctt caccttcgtt
    34801 atcacgtatt gatttaataa attcatttgt gaatttttca cttgatgtgt aaatcacttt
    34861 ggcatctgga ttattatcta aaacatgatg accaatggca tgcattaaat gggtttttcc
    34921 taaaccaaca cctccataga taaataatgg attgtacgct ttggctggtg cttcggccac
    34981 agctaaactc gctgcatgtg gaaagcggtt accaggtcct attacaaaag tgtcaaatgt
    35041 gttatgggca ttgaattgct ctctaccaag cacatgatta tcctcagttg tttcagtaga
    35101 aggttttgtt gtttcttttg gagtagcagt ttcattatta ctataatttg ctaattcttc
    35161 agtagtaata aagtgaggtt ttacttcata gcctacaaca tcaaataaga ttgcttggat
    35221 aatttcagca tattgttgat ttaaccaatt tgcattaaaa ggaatactcg ataatacgat
    35281 agcttcacca tctttgatcg tgtaaagttc agtatctttt aggaaagttg agtaacttac
    35341 agctgataat ttttcttgag caatttcaag cactttttcc caaatttctt tttccgacat
    35401 aaatttactc catctttctt aaatttttaa gttatacaca aaaataacag ccgtggataa
    35461 cttctaaaca caccatgtta ataattatcc acaagttaat aacaatcagt ggataacctt
    35521 atcctgctta ttagttatgc acagtatttt tatgtaaata tcaataaata aatccttata
    35581 aaatcatgag ttggacgact tatccacaca attatacttt ttcagttctc tatatgtcaa
    35641 atacctgtga atatgttgct aaaaatagta taactttgtg tgtaatttct aattatccac
    35701 aattctgaaa actgtaaatg tgcataagtg gataactttt ccttctatag agtatctgtt
    35761 agtgagtgta tcaaaacagc ttgggaaata atttataaag tatgtataag aactgtataa
    35821 ggtattcaaa cattgtaaac actcatgctt cggaccaaac tcatggtgat gttatgaaat
    35881 ttgattgctc gcatcgtgta tttctatctt taatcgataa aagtaaaaaa attgagcagt
    35941 agcataaaat gaacttgcgt tataaaaagg attttgttat attgtagtag ttgcttgaat
    36001 tatgactaga taatcaatga gctaatacga gaattttaat ctaagaaacg caaaatctta
    36061 tctattaaag tgatcagagt ctgcataatc tggtatatta gataactgta taaagatgga
    36121 ggtgttttgc atggtaaaac gtacttatca accaaataaa cgtaaacata gtaaagttca
    36181 tggtttcaga aaacgcatga gcacaaaaaa tggccgtaaa gttttagcgc gccgtcgtcg
    36241 taaaggccgt aaagttttat ctgcataaga tcactgactc atcagtgatc ttttttttcg
    36301 tttaaattaa gaataaatag aaatttatgt tataagctca atagaagttt aaatatagct
    36361 tcaaataaaa acgataaata agcgagtgat gttattggaa aaagcttacc gaattaaaaa
    36421 gaatgcagat tttcagagaa tatataaaaa aggtcattct gtagccaaca gacaatttgt
    36481 tgtatacact tgtaataata aagaaataga ccattttcgc ttaggtatta gtgtttctaa
    36541 aaaactaggt aatgcagtgt taagaaacaa gattaaaaga gcaatacgtg aaaatttcaa
    36601 agtacataag tcgcatatat tggccaaaga tattattgta atagcaagac agccagctaa
    36661 agatatgacg actttacaaa tacagaatag tcttgagcac gtacttaaaa ttgccaaagt
    36721 ttttaataaa aaaattaagt aatgataggg taggggaagg taaacattaa ccactcaaca
    36781 cctcccgaag tcttacctca gacaaacgta agacttacct tagggttata ataacttact
    36841 ttaaaatgta ttcacatcgt tgaaggaggc gagtagcaat atggatttag atacaattac
    36901 gagtatttca acaccaatgg gtgaaggggc aattggaatt gttcgattgt ctggaccgca
    36961 agccgttgaa attgcagaca tattatataa aggaaaacat cttttaaatg atgttccatc
    37021 acatacgatt aattacggtc atattattga tccagagtct aaagaagtgg ttgaagaagt
    37081 tatggtgtct gtgttaagag cgccaaaaac atttacacgc gaagatatta tagaaattaa
    37141 ttgtcatggt ggtatattaa cgattaatag agtgctggaa cttactatga catatggcgc
    37201 tcgaatggct gagccaggtg aatttacaaa acgagccttt ttaaatggtc gtattgattt
    37261 atctcaagct gaagcagtta tggactttat tcgctcgaag acagatagag cttctaaggt
    37321 tgcgatgaat caaattgaag gtcgtctaag tgacttaatc aaaaaacaac gtcaatctat
    37381 attagagata ctcgctcaag tagaagtgaa tattgattat cctgaatacg atgatgttga
    37441 agatgcgact actgaatttc ttttagagca gtctaaagaa atcaaacagg aaattaatcg
    37501 tttattagat accggtgcgc agggtaaaat tatgcgtgaa ggtttatcta cagttattgt
    37561 tggtaaacca aacgtaggta aatcatcgat gttaaataat ttaatacaag ataataaagc
    37621 gattgtaact gaggtagcag gtactacaag agatgtctta gaagagtacg tcaatgttcg
    37681 tggcgtgcca ttaagattag ttgatactgc tggtatacgc gagacagaag atatagtaga
    37741 gaagattggt gttgaacgct ctagaaaggc tcttagccaa gcagacttaa ttttatttgt
    37801 attaaacaat aacgaagcat tgactcaaga agattacaca ttatatgaag tggttaaaaa
    37861 tgaagatgta atcgtaattg ttaataaaat ggatttagag caaaacatag atattaatga
    37921 agttaaagat atgataggtg atacgccatt aattcaaact tcaatgttaa aacaagaagg
    37981 tattgatgaa ttagaaatac aaattcgaga tttgttcttt ggtggagaag tacaaaatca
    38041 ggatatgact tatgtttcta attcaagaca tatttcatta ttaaaacaag caagacaaac
    38101 gatacaagat gcgattgatg cagcagaatc tggtgtgcct atggatatgg tacaaattga
    38161 tttaactaga acttgggaaa tattaggaga aattattggt gagactgcaa gtgatgaact
    38221 catcgatcag ttattcagtc aattctgctt aggtaaatag taattgaaat agacggaata
    38281 ccgtcttaag aaggctagta agatatcaaa taaggaggtt tatattgtgg ttcaagaata
    38341 tgatgtaatc gttataggtg cgggacatgc aggtgtagaa gcaggtttag catctgcaag
    38401 acgtggtgct aaaacattaa tgctaacaat aaatttagat aatattgcat ttatgccatg
    38461 taacccatct gtaggtggac cagctaaagg tatcgttgtt cgtgaaattg atgctttagg
    38521 tggacaaatg gcaaaaacaa tcgataaaac acacattcaa atgagaatgt taaatacagg
    38581 taaaggacct gctgtaagag cactaagagc gcaagcagat aaagtacttt atcaacaaga
    38641 aatgaaacgc gtgattgaag atgaagaaaa tttgcatata atgcaaggta tggtagacga
    38701 acttattata gaagataatg aagttaaagg tgtacgtaca aatattggta cagagtattt
    38761 atctaaagca gtaattatta caacgggaac atttttacgt ggtgaaatca ttttaggtaa
    38821 tatgaagtat tcaagtggac caaatcacca attaccatca atcacattat cagacaattt
    38881 aagagaactt ggttttgata ttgttcgttt taaaacaggt acaccaccgc gtgtaaattc
    38941 aaaaacaatt gactattcga agactgaaat acaaccaggt gacgatgtag gtcgtgcatt
    39001 cagctttgaa acaacagaat atatattaga tcaattgcca tgttggctaa cgtatactaa
    39061 tgctgaaaca cacaaagtta tcgatgataa tttacatcta tctgcaatgt attcagggat
    39121 gattaaagga accgggccac gttattgccc ttcaattgaa gataaatttg ttcgatttaa
    39181 tgataagccg cgacatcaac ttttcttaga gcctgaaggt cgtaatacaa atgaagtata
    39241 tgtgcaagga ttatctacaa gtcttcctga acatgtgcaa cgtcaaatgt tagagacgat
    39301 accaggtctt gaaaaagcag atatgatgcg tgccggctac gcaattgaat atgatgcgat
    39361 tgtgccaacg cagttatggc ctacacttga aacgaaaatg attaaaaact tatacactgc
    39421 aggtcaaatt aatggtacat ctggttatga agaagcagca ggacaaggat tgatggcagg
    39481 tattaacgct gcaggtaaag tgttaaacac aggcgaaaag atattaagtc gttcagatgc
    39541 atatattggt gtcttaatcg atgatcttgt aactaaaggt actaatgaac cttatcgttt
    39601 actaacatca cgtgcagaat atcgtttgtt actacgtcat gataatgctg atttgagatt
    39661 gacggatatg ggatatgaac ttggtatgat ttctgaagaa cgatatgcac gttttaatga
    39721 aaaacgtcag caaattgatg cggaaattaa gcgtttatca gatattcgta ttaaaccaaa
    39781 cgaacatacg caagcgatta ttgaacaaca tggtggttct cgcttaaaag atggtatttt
    39841 agctatcgat ttattacgca gacctgaaat gacttacgat ataattttag aaattttaga
    39901 agaagaacat caattgaatg cagatgttga agaacaagta gaaatacaaa caaaatatga
    39961 aggttatatc aataaatcac tacaacaagt tgagaaagtt aagcgtatgg aagagaagaa
    40021 aattccagaa gacttagatt atagtaagat tgatagtttg gcgactgaag cgcgagaaaa
    40081 attatcagaa gtaaaacctt taaatattgc acaagcttct agaatatcag gggtaaatcc
    40141 agcagacata tctatattat tgatttactt agaacaaggt aaactccaaa gggtgagtga
    40201 ctaatgactg tagaatggtt agcagaacaa ttaaaagaac ataatattga attaactgag
    40261 actcaaaaac aacagtttca aacatattat cgtttacttg ttgaatggaa tgaaaagatg
    40321 aatttgacaa gtattacaga tgaacacgat gtatatttga aacattttta tgattccgtt
    40381 gcacctagtt tttattttga ttttaatcag cctataagta tatgtgatgt aggcgctgga
    40441 gctggttttc caagtattcc gttaaaaata atgtttccgc agttaaaagt gacgattgtt
    40501 gattcattaa ataagcgtat tcaattttta aaccatttag cgtcagaatt acaattacag
    40561 gatgtcagct ttatacacga tagagcagaa acatttggta agggtgtcta cagggagtct
    40621 tatgatgttg ttactgcaag agcagtagct agattatccg tgttaagtga attgtgctta
    40681 ccgctagtta aaaaaggtgg acagtttgtt gcattaaaat cttcaaaagg tgaagaagaa
    40741 ttagaagaag caaaatttgc aattagtgtg ttaggtggta atgttacaga aacacatacc
    40801 tttgaattgc cagaagatgc tggagagcgc cagatgttca ttattgataa aaaaagacag
    40861 acgccgaaaa agtatccaag aaaaccaggg acgcctaata agactccttt acttgaaaaa
    40921 taatgcataa tcctttacaa ctaacataaa aggagcgaat ggataatgaa aaaacctttt
    40981 tcaaaattat ttggtttgaa aaacaaagat gacatcattg gacatattga agaagatcgc
    41041 aatagtaatg ttgaatccat tcaaattgaa cgtatcgttc ccaaccgtta tcaaccaaga
    41101 caggtgtttg aaccaaataa aattaaagaa cttgctgaat caatacatga acatggttta
    41161 ctacaaccta ttgttgtaag accgattgaa gaagatatgt ttgaaattat tgctggagag
    41221 cgccgattta gagcaataca atcactaaat ttacctcaag cagacgttat tattcgtgat
    41281 atggatgatg aagagacggc tgttgttgca ttaattgaga atattcaaag agaaaatttg
    41341 tctgttgttg aagaagcgga agcctataag aaattattgg aaattggtga tacaacgcaa
    41401 agtgaattgg caaaaagttt aggtaaaagt caaagcttta ttgcaaataa gttgcgttta
    41461 ttgaagttgg cgccgaaagt actacttcgc ttaagagaag gtaaaattac tgaacgccat
    41521 gcgagagcgg tattatcatt gtctgatagc gaacaagaag cgttgattga gcaagtcatt
    41581 gcacaaaagc taaatgtgaa acagactgaa gatagagtac gccaaaaaac ggggcccgaa
    41641 aaagtcaaag cacaaaacct tcgctttgca caagatgtca ctcaagcacg agatgaggta
    41701 ggcaaaagta tccaagcgat tcaacaaaca ggactacatg ttgagcataa agacaaagat
    41761 catgaagatt attatgaaat aaaaattcga atatataaac gttagtagta ggatgtcgta
    41821 tacatgatga ctaacacata aaagacaaag ctaagatcat aacagctttg tctttttttt
    41881 ctt
//
LOCUS       DAHHOJ010000009        41475 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.009, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000009 DAHHOJ010000000
VERSION     DAHHOJ010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 41475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41475
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.009"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            70..879
                     /locus_tag="NBH32_000390"
     CDS             70..879
                     /locus_tag="NBH32_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000835976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1514025.1"
                     /translation="MEYIKKIALYMSVLLLIIFIGGCGNMKEEQKKEANTNKTDSKEE
                     QIKKSFAKTLDMYPTENLEDFYNKEGYRDGEFKKDDKGTWLIRSEIVKQPKGKVMKTR
                     GMQLYINRNTKTAKGFFVLKEISENNNRVNKDKEEKYEVKMVGNKIIPTEQINDEKIK
                     KEIENFKFFVQYGKFKNFEKYKNGEFSYNPEAPIYSAKYQLQNDDYNVRQLRKKYDIS
                     TKEAPKLLLKGGGDLKGSSVGQKDIEFTFVERKGENIYFNDSVEFIPSEDK"
     gene            882..2210
                     /locus_tag="NBH32_000391"
     CDS             882..2210
                     /locus_tag="NBH32_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514026.1"
                     /translation="MSKPEYQLNPGKINANTEETSAISYFSYQIENANISGLDKVKMY
                     QQIDILKDRYKFPSNLEYVDSYTDSLTGVTTSAFLNKDTGKVTLGMTGTNLQDEAFKK
                     LKEGEFSRQNFTNALETVKDGYADLKILYSPASDQNYRYANTQEFINKIKSKYDIDFI
                     TGHSLGGRDAVVLGMSNGIPNIVVYNPAPISITSLNPNSPDGKRLLELYKNYKGNITR
                     FVAENDALTENLKKYKHYVFFGNDKVFKNGKGHGMEGFLTKEEQKAIKKELKKLQGYA
                     EENNKSFVKHSNNAISKLASIELLRANMMTTNGGGLSSSQQKVLESLTALTIAQSFSQ
                     LIDDEINQIKKMYNEKKKKFGKNWEDAQKAGKAVGEDLSVNGVLNALDEGQVNESSMV
                     REPEQMISAKERQLSTIGSSVSNYIMRVRLSINEIVDKDQVLASQIGGLL"
     gene            2207..2530
                     /locus_tag="NBH32_000392"
     CDS             2207..2530
                     /locus_tag="NBH32_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514027.1"
                     /translation="MMFNQINNKNELEESYESEKKRIENELQNLNELRHRTRKENERS
                     YDVFQYLKHEMNYSEDAQRKMTRNIEAYEQEINEIIRKQEWKLEEYKEDLKKSYEKHL
                     DKLSD"
     gene            2549..2863
                     /locus_tag="NBH32_000393"
     CDS             2549..2863
                     /locus_tag="NBH32_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514028.1"
                     /translation="MTLIEPDMNLRMPDISTTVETLNLISKMEAQKENIRTVIAPEHK
                     HKYKDIENGLKGEEKVLIEQMAQHCEAFKANFKGAAQGDWVKSAMSEIDSIKDDLKKI
                     NS"
     gene            complement(3191..3451)
                     /locus_tag="NBH32_000394"
     CDS             complement(3191..3451)
                     /locus_tag="NBH32_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514029.1"
                     /translation="MSALLYNGDKDISHERESRCINMLFVILVLYVIGIAFILLSVFG
                     SKTEGLSTKHTLYTIGSAIITIAIFISIGYAIQYLTAALYGL"
     gene            3570..4772
                     /locus_tag="NBH32_000395"
     CDS             3570..4772
                     /locus_tag="NBH32_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1514030.1"
                     /translation="MAKIPVTVLSGYLGSGKTTLLNHILQNREGRRIAVIVNDMSEVN
                     IDKDLVADGGGLSRTDEKLVELSNGCICCTLRDDLLKEVERLVKKGGIDQIVIESTGI
                     SEPVPVAQTFSYIDDELGIDLTAICRLDTMVTVVDANRFVHDINSEDLLMDRDQSVDE
                     TDERSIADLLIDQVEFCDVLIINKIDLISEEELAKLEKVLSALQPTAKIIKTTNSEVD
                     LKEVLNTQRFDFEKASESAGWIKELESGGHASHTPETEEYGISSFVYKRRLPFHAKRF
                     NDWLESMPNNVVRSKGIIWLAQYNHVACLLSQAGSSCNIHPVTYWVASMSEAQQTQIL
                     AERQDVAAEWDPEYGDRHTQFVIIGTELDEEKLTKELDACLVNAQEIDADWQQFEDPY
                     QWQIRPAR"
     gene            complement(5430..5492)
                     /locus_tag="NBH32_000396"
     CDS             complement(5430..5492)
                     /locus_tag="NBH32_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-4"
                     /protein_id="HCD1514031.1"
                     /translation="MAIVGTIIKIIKAIIDIFAK"
     gene            complement(5556..5624)
                     /locus_tag="NBH32_000397"
     CDS             complement(5556..5624)
                     /locus_tag="NBH32_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-3"
                     /protein_id="HCD1514032.1"
                     /translation="MEFVAKLFKFFKDLLGKFLGNN"
     gene            complement(5676..5741)
                     /locus_tag="NBH32_000398"
     CDS             complement(5676..5741)
                     /locus_tag="NBH32_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-2"
                     /protein_id="HCD1514033.1"
                     /translation="MGIIAGIIKFIKGLIEKFTGK"
     gene            complement(5773..5838)
                     /locus_tag="NBH32_000399"
     CDS             complement(5773..5838)
                     /locus_tag="NBH32_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin PSM-alpha-1"
                     /protein_id="HCD1514034.1"
                     /translation="MGIIAGIIKVIKSLIEQFTGK"
     gene            6359..7843
                     /locus_tag="NBH32_000400"
     CDS             6359..7843
                     /locus_tag="NBH32_000400"
                     /EC_number="1.6.5.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit 5"
                     /protein_id="HCD1514035.1"
                     /translation="MLSFQLLFSLFVIALIIALISGLLFLAPVMLMRYIKLHLYILVM
                     PVLFAVIGFFGIHGQHVLGPFKIDRLSWLLAGFVMALGFIIQKFSMRYLLGDHHYRHY
                     FPLFTAITSFASLAWMSEDLRLMALCWGMTLLCLTLLMNVNRFWKVPRESAKLSSMTF
                     LCGWLAFVGAIVTIYIATGEWRVPQHMSNSTWSLLTNVLLVLAVMIPAAQFPFHRWLI
                     ESVTAPTPVSAIMHAGIVNAGGVILTRFAPIFDNGFALSLLLILSSISVLLGSGISLV
                     QVDYKRQLVGSTMSQMGFMLVQCALGVYSAAIIHLILHGIFKATLFLQSGSIVKRFNI
                     PKQASAKDAYGWIVMGRVLAIIVAFLFWMSSDRSAYEVLSALILAWSLLVSWNQMVAF
                     SKGRMARLVGMILIAIVTFIYIITHNYFYDVLRNITTHATTPPTVSVIISVVILIFGS
                     LLSIWVARHRYSKGFAVLYVWLVNLGEARSKAIESHPNYLKKYL"
     gene            7856..10561
                     /locus_tag="NBH32_000401"
     CDS             7856..10561
                     /locus_tag="NBH32_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbcC family protein"
                     /protein_id="HCD1514036.1"
                     /translation="MTTQLNINSVIENAKRVITPLSPISIFAARNPWEGLEADTFEDV
                     AKWLRDVRDVDIFPNKALIESAVARGELDESVFNQLVTDMLLEHHYNIPQHYINLYID
                     NIKTLKDVPTSYMNHSNVDVVADLLLEKSKRDMAESYHHYDVRPMSDAIIDEQGEPLS
                     EQVNRQMIKWTKLYIDQFLSSWTMPKREQSFYHAWLHLAQHDHSFTKAQRQVIKGLPN
                     DPEMTIESVLTHFSIAQEDYQACVEGHLLALPGWAGMLYYRSQQHHFEQHLLTDYLAI
                     RLVVEQLLVGDEFKSVTKDCESRSENWFKQTVASWCYYSDMPSDVLLQHDVNEIQTFI
                     HFAATMNKNVFKNLWLIAWEMTYESQLKQKIKAGHESVAGALDVNQVNVLENDNANQP
                     HSVLLNDTQAVDENNSELNQMGTSTKAQIAFCIDVRSEPFRRHIEAAGPFETIGIAGF
                     FGLPIQKDAVDEQFKHDSLPVMVPPAYRIKEFADRYDMNVYRQQQQTMSSMFYTFKLM
                     KNNVMPSLLLPELSGPFLSLSTIVNSIMPRKSRASLQKIKQKWLKKPETKLTIDREFD
                     RTSDLPVGFTEQEQIDFALQALKLMDLTEAFAPFVVLAGHASHSHNNPHHASLECGAC
                     GGASSGFNAKLLAMICNRPNVRQGLKQAGVVIPETTVFAAAEHHTSTDTLAWVYVPDT
                     LSALALDAYESLNDAMPMISEHANRERLDKLPTIGRVNHPVEEAQRFASDWSEVRPEW
                     GLAKNASFIIGRRQLTKGIDLEGRTFLHNYDWRKDKDGTLLNTIISGPALVAQWINLQ
                     YYASTVAPHFYGSGNKATQTVTSGVGVMQGNASDLMYGLSWQSVMAADRTMYHSPIRL
                     LVVVQAPDYVVARLLANNDHFARKVSNHWLRLMSVNEEGRFKSWI"
     gene            10723..11085
                     /locus_tag="NBH32_000402"
     CDS             10723..11085
                     /locus_tag="NBH32_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2294 domain-containing protein"
                     /protein_id="HCD1514037.1"
                     /translation="MKRTKGEIEAEISKAITQWEKDFLGRGSLSVKSDILRDMVIISL
                     QGILTPAEYRVCSTNEGLLNIKRTRSELVESGEQDLNDIILKITGIKVMSFHSDLSTV
                     TGERIIVFKLEDNLEKHI"
     gene            complement(11314..11730)
                     /locus_tag="NBH32_000403"
     CDS             complement(11314..11730)
                     /locus_tag="NBH32_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514038.1"
                     /translation="MLSIISMCHKFHFTDNIKIIKFILLCILQIKHCIIEITIFCYFQ
                     FSTIYSYYTEGVRRINRVIFVYIALIITLVRFFTPIHPSFSNLIYWIFVLYFIPIILC
                     VIGFKAEKLIATMVIIPNFLGILYRLYAYVTHILFM"
     gene            11768..12442
                     /locus_tag="NBH32_000404"
     CDS             11768..12442
                     /locus_tag="NBH32_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCD1514039.1"
                     /translation="MIDKKLTSPKMTVPLFLIALIVFIGMFYSVVTNQEWLKNIDMGS
                     LTWFTDYFGEPQRQYVNNLFNYYMTFSAEIGDVKGVVLISIIITIILFIKQRHLAVWF
                     VTYLVSGVIMNKLIKDTVLRPRPYNHLAVDTGFSFPSGHSNASTLLYFALMIIIISLA
                     AKTITKVLSALVMGILWLSILFCRLYFHAHYFSDVIGGTSLAIIWVALFLMVYPYFIN
                     HRRQRV"
     gene            12479..13213
                     /locus_tag="NBH32_000405"
     CDS             12479..13213
                     /locus_tag="NBH32_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD1514040.1"
                     /translation="MRIKTPSPSYLKGTNGHAILLLHSFTGTNRDVKHLAAELNAQGF
                     SCYAPNYPGHGLLLKDFMTYNVDDWWEEVEKAYQFLVNEGYESISATGVSLGGLMTLK
                     LAQHYPLKRIAVMSAPKEKSDDGLIEHLVYYSQRMSNILNLDQQASSAQLAAIDDYEG
                     EITKFQHFIDDIMTNLNVIKMPANILFGGKDAPSYETSAHFIYEHLGSVDKELNGLKD
                     SHHLMTHGEGRDILEENVIRFFNALT"
     gene            13342..13455
                     /locus_tag="NBH32_000406"
     CDS             13342..13455
                     /locus_tag="NBH32_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514041.1"
                     /translation="MGIVPEVVMWRDVGDIDGVVCGEILAYVYSVEPENLM"
     gene            13620..14957
                     /locus_tag="NBH32_000407"
     CDS             13620..14957
                     /locus_tag="NBH32_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HCD1514042.1"
                     /translation="MKRQQSQWKSSTGFILASAGSAIGLGAMWKFPYMAGIYGGGAFL
                     AMFLIFTIFVGLPLLIMEFTVGKMGRTYTTQIYSKLTGKKWLNIIGWNGNLAVFILFG
                     FYSVIGGWIVIYIGQVLWQLVIFQRINHLQEMNFEAVISNPWLTVLGQGIFIFATMII
                     VMLGVEKGLEKASKVMMPLLFVFLIIIVIKSLTLDGALEGVKFILQPRVSEITADGIL
                     FALGQSFFTLSLGTTGMITYASYASKDMTIKSSAISIVVMNIFVSVLAGLAIFPALHS
                     FGYEPQEGPGLLFKVLPMVFSQMHLGTLFYLGFLVLFLFAALTSSISLLELNVSNFTK
                     NDNTKRKKVAVIGSILVFIISIPATLSFGILKDVRFGAGTIFDNMDFIVSNVLMPLGA
                     LGTTLVVGQLLDKKLLQQYFGKDRFRLFSGWYYLIKYAMPVVIILVFIVQLFS"
     gene            15174..16079
                     /locus_tag="NBH32_000408"
     CDS             15174..16079
                     /locus_tag="NBH32_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000634108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HCD1514043.1"
                     /translation="MITYDLIGNTPLVLLEHYSDDKVKIYAKLEQWNPGGSVKDRLGK
                     YLVEKAIQEGRVRAGQTIVEATAGNTGIGLAIAANRHHLKCKIFAPYGFSEEKINIMI
                     ALGADVSRTSQSEGMHGAQLAARSYAEKYGAVYMNQFESEHNPDTYFHTLGPELTSAL
                     QQIDYFVAGIGSGGTFTGTARYLKQHHVQCYAVEPEGSVLNGGPAHAHDAEGIGSEKW
                     PIFLERRLVDGIFTIKDQDAFRNVKSLAINEGLLVGSSSGAALQGALNLKAQLSEGTI
                     VVVFPDGSDRYMSKQIFNYEENDYE"
     gene            16072..17214
                     /locus_tag="NBH32_000409"
     CDS             16072..17214
                     /locus_tag="NBH32_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional cystathionine
                     gamma-lyase/homocysteine desulfhydrase"
                     /protein_id="HCD1514044.1"
                     /translation="MNKKTKLIHGGHTTDDYTGAVTTPIYQTSTYLQDDIGDLRQGYE
                     YSRTANPTRSSVESVIAALENGKHGFAFSSGVAAISAVVMLLDKGDHIILNSDVYGGT
                     YRALTKVFTRFGIEVDFVDTTHTDSIVQAIRPTTKMLFIETPSNPLLRVTDIKKSAEI
                     AKEHGLISVVDNTFMTPYYQNPLDLGIDIVLHSATKYLGGHSDVVAGLVATSDDKLAE
                     RLAFISNSTGGILGPQDSYLLVRGIKTLGLRMEQINRSVIEIIKMLQAHPAVQQVFHP
                     SIESHLNHDVHMAQADGHTGVIAFEVKDTESAKQLIKATSYYTLAESLGAVESLISVP
                     ALMTHASIPADIRAKEGITDGLVRISVGIEDTEDLVDDLKQALDTL"
     gene            17508..18533
                     /locus_tag="NBH32_000410"
     CDS             17508..18533
                     /locus_tag="NBH32_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCD1514045.1"
                     /translation="MIEFRQVSKTFNKKKQKIHALKDVSFKVNRNDIFGVIGYSGAGK
                     STLVRLVNHLEAASSGQVLVDGHDITNYSEKGMREIKKDIGMIFQHFNLLNSATVFKN
                     VAMPLILSKKSKTEIKQRVTEMLEFVGLSDKKDQFPDELSGGQKQRVAIARALVTNPK
                     ILLCDEATSALDPATTASILTLLKNVNQTFGITIMMITHEMRVIKDICNRVAVMEKGQ
                     VVETGTVKEVFSHPKTTIAQNFVSTVIQTEPSTSLIRRLNDEQVGDFKDYKIFVEETQ
                     VTQPIINDLIQICGREVKILFSSMSEIQGNTVCYMWLRFNMDQQFDDTAINQYFKEKN
                     IQFEEVH"
     gene            18537..19196
                     /locus_tag="NBH32_000411"
     CDS             18537..19196
                     /locus_tag="NBH32_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1514046.1"
                     /translation="MFGSDLDSAQLLQALYETLYMVSIALFLGAVIGIPLGVLLVITR
                     KQGIWPNIVIHQVLNPLINILRSLPFIILLIAIVPFTKLVVGTSIGTTAAIVPLTVYV
                     APYIARLVENSLLEVDEGIIEAAKAMGASPLQIIRYFLIPEALGSLVLAITTAIIGLI
                     GSTAMAGAVGGGGIGDLALVYGYQRFDTTVIIITVIVLVIIVQVIQTLGNVLARFIRR
                     H"
     gene            19233..20075
                     /gene="gmpC"
                     /locus_tag="NBH32_000412"
     CDS             19233..20075
                     /gene="gmpC"
                     /locus_tag="NBH32_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter
                     glycylmethionine-binding lipoprotein"
                     /protein_id="HCD1514047.1"
                     /translation="MKRLIGLVIVALVLLAACGSNNDKKVTIGVASNDTKAWEKVKEL
                     AKKDDIDVEIKHFSDYNLPNKALNDGDIDMNAFQHFAFLDQYKKAHKGTKISALSTTV
                     LAPLGIYSDKIKDVKKVKDGAKVVIPNDVSNQARALKLLEAAGLIKLKKDFGLAGTVK
                     DITSNPKHLKITAVDAQQTARALSDVDIAVINNGVATKAGKDPKNDPIFLEKSNSDAV
                     KPYINIVAVNDKDLDNKTYAKIVELYHSKEAQKALQEDVKDGEKPVNLSKDEIKAIET
                     SLAK"
     gene            20408..21415
                     /gene="aaa"
                     /locus_tag="NBH32_000413"
     CDS             20408..21415
                     /gene="aaa"
                     /locus_tag="NBH32_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin/adhesin Aaa"
                     /protein_id="HCD1514048.1"
                     /translation="MQKKVIAAIIGTSAISAVAATQANAATTHTVKPGESVWAISNKY
                     GISIAKLKSLNNLTSNLIFPNQVLKVSGSSNSTSNSSRPSTNSGGGSYYTVQAGDSLS
                     LIASKYGTTYQNIMRLNGLNNFFIYPGQKLKVSGTASSSNSASNSSRPSTNSSGGSYY
                     TVQAGDSLSLIASKYGTTYQKIMSLNGLNNFFIYPGQKLKVTGNATSSNSASATTTNR
                     GYNTPVFSHQNLYTWGQCTYHVFNRRAEIGKGISTYWWNANNWDNAAAADGYTIDNRP
                     TVGSIAQTDVGYYGHVMFVERVNNDGSILVSEMNYSAAPGILTYRTVPAYQVNNYRYI
                     H"
     gene            complement(21600..21869)
                     /locus_tag="NBH32_000414"
     CDS             complement(21600..21869)
                     /locus_tag="NBH32_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514049.1"
                     /translation="MAKRSKSQRLSSLLNVAGFIVDGYNGYKYHAKNKKLVYLSLGLS
                     TVGTVLDFYISIKSPRKFKKAVAVVTLITNGARLFTSIRKVKHEY"
     gene            22018..22413
                     /locus_tag="NBH32_000415"
     CDS             22018..22413
                     /locus_tag="NBH32_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD1514050.1"
                     /translation="MIKCVCLVEETADKILLVQVRNREKYYFPGGKIEEGESQVQALL
                     REVKEELNLTLTMDEIEYIGTIVGPAYPQQDMLTELNGFRALTKIDWENVTINNEITD
                     IRWMDKDNDALIAPAVKVWIETYGGKHDK"
     gene            22403..22888
                     /locus_tag="NBH32_000416"
     CDS             22403..22888
                     /locus_tag="NBH32_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1514051.1"
                     /translation="MTNNDTIMLRHYVPQDYSMLEAFQLSESDLKFVKTPEENITAAM
                     SDNERYPIVVMDGRQCVAFFTLHRGKGVAPFSDNQDAVFFRSFSVDQRYRNRGIGKVV
                     MEKLASFITSTFQGINEIVLTVNTDNPHAMALYRQQGYQYMGDSMFVGRPVHIMALTI
                     K"
     gene            complement(23057..23839)
                     /locus_tag="NBH32_000417"
     CDS             complement(23057..23839)
                     /locus_tag="NBH32_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD1514052.1"
                     /translation="MNAVFILALLLLFLMIIFGGKKGFISYLTLFLNFAILIISIVLI
                     IFGVPIYVVTFFFCIIIGACNLFVLNSYNVKTQAAFISTIVTTILLITLIYLSVYFGH
                     LQGFSTEQQDETYVYSMNIGIDMVQFMVFTIILAVIAAVIDLTITISSPIYELHQVNP
                     TLTQYELFQSGMRVGREILATSANTIYLAFFGGQLALFIWFFKLKYSFGHIINSKIFA
                     QEFISIILGGIAVAISIPITAWITAFLIKRPSRQRKNDESIN"
     gene            complement(23836..24954)
                     /locus_tag="NBH32_000418"
     CDS             complement(23836..24954)
                     /locus_tag="NBH32_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HCD1514053.1"
                     /translation="MFLKYRNLFTKPFNIILGLFCIAFIGAILFTFNNENFYNKPIGQ
                     IIDVKHVSSTPTKDAQNNRDIKYKNQLKVKILNGQFAGETTTINHQYVKSQADSEAFR
                     THEKVLLHISNKPSDAYIIEKKRDTLTVIITGLFLLTVLLVGRKVGLQSILSLILNSI
                     AILIAIYIHIQHSNINLFLLMTIAMICSTVLTLLLVTGWHMRTLITIASTIIGTFLSI
                     GLTELIIYMTDGKGIKYETMNFLSLPPKDIFLASVLIGSLGAIMDVAITIASGMHEIL
                     QRTPHISMRRWALAGRYIGQDIMGTMTNILLFSYLSGALPMFLIFLKNANTVTYTISM
                     NWSLEIARALTGGIGIVLTIPITILFMEIFETLRRAKQ"
     gene            complement(25069..25953)
                     /locus_tag="NBH32_000419"
     CDS             complement(25069..25953)
                     /locus_tag="NBH32_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1514054.1"
                     /translation="MEIKQLRYFIEVAKREHISETALELNIAQSAISRQITLLEQELN
                     VSLFKKQGRNITLTSEGKLLFNEALRIIEHLDSTIEQFQSHGLTKNKSIYIGYDESDV
                     SHMLLPLIQTFHLQNDTHVIPNLLEHDTIINSVLNGNIDIGFTELTPEIRKHKQLHML
                     PLFEEHYHLYAPSDDPITMATHPPLIQFEHSHIYCLAPFAETVKKQLRKITKSDVYTI
                     SSQPLAQYLLRQKEGYIISSQNIHLPESKSWIDIKLDHTELKRTICAITKEPYTKSDI
                     GILLTLIQQLMTKTSTFH"
     gene            26134..30633
                     /gene="gltB"
                     /locus_tag="NBH32_000420"
     CDS             26134..30633
                     /gene="gltB"
                     /locus_tag="NBH32_000420"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCD1514055.1"
                     /translation="MHNEKLIKGLYDYREEHDACGIGFYANMDNKRSHDIIDKSLEML
                     RRLDHRGGVGADGITGDGAGIMTEIPFAFFKQHVTDFDIPGEGEYAVGLFFSKERVLG
                     SEHEAVFKKYFEGEGLSILGYRNVPVNKDAIAKHVADTMPVIQQVFIDIRDIEDVEKH
                     LFLARKQLEFYSTQCDLELYFTSLSRKTIVYKGWLRSDQIKKLYTDLSDDLYQSKLEL
                     VHSRFSTNTFPSWKRAHPNRMLMHNGEINTIKGNVNWMRARQHKLIETLFGEDQHKVF
                     QIVDEDGSDSAIVDNALEFLSLAMEPEKAAMLLIPEPWLYNEANDANVRAFYEFYSYL
                     MEPWDGPTMISFCNGDKLGALTDRNGLRPGRYTITKDNFIVFSSEVGVVDVPESNVAF
                     KGQLNPGKLLLVDFKQNKVIENNDLKGAIAGELPYKAWIDNHKVDFDFENIQYQDSQW
                     KDETLFKLQRQFAYTKEEIHKYIQELVEGKKDPIGAMGYDAPIAVLNERPESLFNYFK
                     QLFAQVTNPPIDAYREKIVTSELSYLGGEGNLLAPDETVLDRIQLKRPVLNESHLAAI
                     DQEHFKLTYLSTVYEGDLEDALEALGREAVNAVKQGAQILVLDDSGLVDSNGFAMPML
                     LAISHVHQLLIKADLRMSTSLVAKSGETREVHHVACLLAYGANAIVPYLAQRTVEQLT
                     LTEGLQGTVVDNVKTYTDVLSEGVIKVMAKMGISTVQSYQGAQIFEAIGLSHDVIDRY
                     FTGTQSKLSGISIDQIDAENKARQQSDDNYLASGSTFQWRQQGQHHAFNPESIFLLQH
                     ACKENDYAQFKAYSEAVNKNRTDHIRHLLEFKACTPIDIDQVEPVSDIVKRFNTGAMS
                     YGSISAEAHETLAQAMNQLGGKSNSGEGGEDAKRYEVQVDGSNKVSAIKQVSSGRFGV
                     TSDYLQHAKEIQIKVAQGAKPGEGGQLPGTKVYPWIAKTRGSTPGIGLISPPPHHDIY
                     SIEDLAQLIHDLKNANKDADIAVKLVSKTGVGTIASGVAKAFADKIVISGYDGGTGAS
                     PKTSIQHAGVPWEIGLAETHQTLKLNDLRSRVKLETDGKLLTGKDVAYACALGAEEFG
                     FATAPLVVLGCIMMRVCHKDTCPVGVATQNKDLRALYRGKAHHVVNFMHFIAQELREI
                     LASLGLKRVEDLVGRTDLLQRSSTLKANSKAASIDVEKLLCPFDGPNTKEIQQNHNLE
                     HGFDLTNLYEVTKPYITEGRRYTGSFTVNNEQRDVGVITGSEISKQYGEAGLPENTIN
                     VYTNGHAGQSLAAYAPKGLMIHHTGDANDYVGKGLSGGTVIVKAPFEERQNEIIAGNV
                     SFYGATGGKAFINGSAGERFCIRNSGVDVVVEGIGDHGLEYMTGGHVINLGDVGKNFG
                     QGMSGGIAYVIPSDVEAFVENNQLDTLSFTKIKHQEEKAFIKQMLEEHVSHTNSTRAI
                     HVLKHFDRIEDVVVKVIPKDYQLMMQKIHLHKSLHDNEDEAMLAAFYDDSKTIDAKHK
                     PAVVY"
     gene            30651..32114
                     /locus_tag="NBH32_000421"
     CDS             30651..32114
                     /locus_tag="NBH32_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HCD1514056.1"
                     /translation="MGEFKGFMKYDKQYLGELSLVDRLKHHKAYQQRFTKEDASIQGA
                     RCMDCGTPFCQTGQQYGRETIGCPIGNYIPEWNDLVYHQDFKTAYERLSETNNFPDFT
                     GRVCPAPCESACVMKINRESIAIKGIERTIIDEAFENGWVAPKVPSRRRDEKVAIVGS
                     GPAGLTAAEELNLLGYQVTIYERARESGGLLMYGIPNMKLDKDVVRRRIKLMEEAGIT
                     FINGVEVGVDIDKATLESEYDAIILCTGAQKGRDLPLEGRMGDGIHFAMDYLTEQTQL
                     LNGEIDDITITAKDKNVIIIGAGDTGADCVATALRENCKSIVQFNKYTKLPEAITFTE
                     NASWPLAMPVFKMDYAHQEYEAKFGKEPRAYGVQTMRYDVDDKGHIRGLYTQILEQGE
                     NGMVMKEGPERFWPADLVLLSIGFEGTEPTVPNAFNIKTDRNRIVADDTNYQTNNEKV
                     FAAGDARRGQSLVVWAIKEGRGVAKAVDQYLASKVCV"
     gene            32383..32475
                     /locus_tag="NBH32_000422"
     tRNA            32383..32475
                     /locus_tag="NBH32_000422"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:32419..32421,aa:Ser,seq:tga)
     gene            32946..34373
                     /gene="treP"
                     /locus_tag="NBH32_000423"
     CDS             32946..34373
                     /gene="treP"
                     /locus_tag="NBH32_000423"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HCD1514057.1"
                     /translation="MAVKREDVKAIVTAIGGKENLEAATHCVTRLRLVLKDESKVDKD
                     ALSNNALVKGQFKADHQYQIVIGPGTVDEVYKQFIDETGAQEASKDEAKKAAAQKGNP
                     VQRLIKLLGDIFIPILPAIVTAGLLMGINNLLTMKGLFGPKALIEMYPQIADISNIIN
                     VIASTAFIFLPALIGWSSMRVFGGSPILGIVLGLILMHPQLVSQYDLAKGNIPTWNLF
                     GLEIKQLNYQGQVLPVLIAAYVLAKIEKGLNKVVHDSIKMLVVGPVALLVTGFLAFII
                     IGPVALLIGTGITSGVTFIFQHAGWLGGAIYGLLYAPLVITGLHHMFLAVDFQLMGSS
                     LGGTYLWPIVAISNICQGSAAFGAWFVYKRRKMVKEEGLALTSCISGMLGVTEPAMFG
                     VNLPLKYPFIAAISTSCVLGAIVGMNNVLGKVGVGGVPAFISIQKEFWPVYLIVTAIA
                     IVVPCILTIVMSHFSKQKAKEIVED"
     gene            34438..36078
                     /gene="treC"
                     /locus_tag="NBH32_000424"
     CDS             34438..36078
                     /gene="treC"
                     /locus_tag="NBH32_000424"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCD1514058.1"
                     /translation="MSKEIDWRKSVVYQIYPKSFNDTTGNGIGDINGIIEKLDYIKLL
                     GVDYIWLTPVYESPMNDNGYDISNYLEINEDFGTMDDFEKLIKVAHQKDLKVMLDIVI
                     NHTSTEHEWFKEARKSKDNPYRDYYFFRSSEDGPPTNWHSKFGGNAWKYDSETDEYYL
                     HLFDVSQADLNWDNPEVRQSLYRIVNHWIDFGVDGFRFDVINLISKGEFKDSDKIGKE
                     FYTDGPRVHEFLHELNRQTFGNTDMMTVGEMSSTTIENCIKYTQPERQELNSVFNFHH
                     LKVDYVDGEKWTNAKLDFHKLKEILMQWQRGIYDGGGWNAIFWCNHDQPRVVSRFGDD
                     TSEEMRIQSAKMLAIALHMLQGTLYIYQGEEIGMTDPHFTSIAQYRDVESINAYHQLL
                     SEGHAEADVLAILGQKSRDNSRTPMQWSDDVNAGFTAGKPWIDISENYHQVNVRQALQ
                     NKDSIFYTYQKLIQLRHTHDIITYGDIVPRFMDHDHLFVYERHYKNQQWLVIANFSAS
                     AVDLPEGLAREGRVVIQTGTVENNTISGFGAIVIETNA"
     gene            36103..36831
                     /gene="treR"
                     /locus_tag="NBH32_000425"
     CDS             36103..36831
                     /gene="treR"
                     /locus_tag="NBH32_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HCD1514059.1"
                     /translation="MAKQKKFMKIYEALKEDILNGQIQYGEQIPSEHDLVQLYQSSRE
                     TVRKALDLLALDGMIQKIHGKGSLVIYQEVTEFPFSELVSFKEMQEEMGVAYLTEVVV
                     NEVVEAHEVPEVQHALNINSSESLIHIVRTRRLNQHVKIVDEDYFLKSIVSDIGNDVA
                     SDSIYDYLEKVLNLNISYSSKSITFEPFDEQAYQLFGDVSVAYSATVRSIVYLENTMP
                     FQYNISKHLANEFKFNDFSRRRTK"
     gene            36893..37162
                     /gene="ffs"
                     /locus_tag="NBH32_000426"
     ncRNA           36893..37162
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="NBH32_000426"
                     /product="signal recognition particle sRNA large type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01854"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF01854"
     gene            37235..37336
                     /locus_tag="NBH32_000427"
     CDS             37235..37336
                     /locus_tag="NBH32_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514060.1"
                     /translation="MKTIQRIIRGTCLWEVAFLYVKFDSSELDVQFE"
     gene            37474..37998
                     /locus_tag="NBH32_000428"
     CDS             37474..37998
                     /locus_tag="NBH32_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1514061.1"
                     /translation="MQIYLSTLTELDYDKSLNSIEESFDDNPETSWQARAKVKHLRKS
                     PCYNFELEVIAKNENNDVVGHVLLIEVEINSDDKTYYGLAIASLSVHPELRGQKLGRG
                     LVQAVEERAKAQEYSTVVVDHCFDYFEKLGYQNAAEHDIKLESGDAPLLVKYLWDNLT
                     DAPHGIVKFPEHFY"
     gene            38067..39764
                     /gene="dnaX"
                     /locus_tag="NBH32_000429"
     CDS             38067..39764
                     /gene="dnaX"
                     /locus_tag="NBH32_000429"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCD1514062.1"
                     /translation="MNYQALYRMYRPQSFEDVVGQEHVTKTLRNAISKEKQSHAYIFS
                     GPRGTGKTSIAKVFAKAINCLNSTDGEPCNECHICKGITQGTNSDVIEIDAASNNGVD
                     EIRNIRDKVKYAPSESKYKVYIIDEVHMLTTGAFNALLKTLEEPPAHAIFILATTEPH
                     KIPPTIISRAQRFDFKAISLDQIVERLKFVADAQQIECEDAALAFIAKASEGGMRDAL
                     SIMDQAIAFGDGTLTLQDALNVTGSVHDEALDHLFDDIVQGDVQASFKKYHQFITEGK
                     EVNRLINDMIYFVRDTIMNKTSEKDTEYRALMNLELDMLYQMIDLINDTLVSIRFSVN
                     QNVHFEVLLVKLAEQIKGQPQVIANVAEPAQIASSPNTDVLLQRMEQLEQELKTLKAQ
                     GVSVAPTQKSSKKPVRGIQKSKNAFSMQQIAKVLDKANKADIKLLKDHWQEVIDHAKN
                     NDKKSLVSLLQNSEPVAASEDHVLVKFEEEIHCEIVNKDDEKRSSIESVVCNIVNKNV
                     KVVGVPSDQWQRVRTEYLQNRKNEGDDMPKQQAQQTDIAQKAKDLFGEETVHVIDEE"
     gene            39854..40171
                     /locus_tag="NBH32_000430"
     CDS             39854..40171
                     /locus_tag="NBH32_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002479279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCD1514063.1"
                     /translation="MRGGGNMQQMMKQMQKMQKKMAQEQEKLKEERIVGTAGGGMVAV
                     TVTGHKEVVDVEIKEEAVDPDDIEMLQDLVLAATNEAMNKADELTQERLGKHTQGLNI
                     PGM"
     gene            40178..40774
                     /gene="recR"
                     /locus_tag="NBH32_000431"
     CDS             40178..40774
                     /gene="recR"
                     /locus_tag="NBH32_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCD1514064.1"
                     /translation="MHYPEPISKLIDSFMKLPGIGPKTAQRLAFHTLDMKEDDVVQFA
                     KALVDVKRELTYCSVCGHITENDPCYICEDKQRDRSVICVVEDDKDVIAMEKMREYKG
                     LYHVLHGSISPMDGIGPEDINIPSLIERLKSDEVSELILAMNPNLEGESTAMYISRLV
                     KPIGIKVTRLAQGLSVGGDLEYADEVTLSKAIAGRTEM"
     gene            40979..41084
                     /locus_tag="NBH32_000432"
                     /pseudo
     CDS             40979..41084
                     /locus_tag="NBH32_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        14017 a         5995 c         8492 g        12971 t
ORIGIN      
        1 atcactattt aattaagttg tatgaaatat tcaaatgttt ataaaattaa agcagagaga
       61 tgtgaaatga tggagtatat aaaaaaaatt gctttgtaca tgagtgtatt acttttaatc
      121 atttttattg ggggatgtgg aaatatgaaa gaagaacaga aaaaagaagc aaatacaaat
      181 aaaacagatt caaaagaaga gcaaatcaaa aaaagctttg cgaaaacgtt ggatatgtat
      241 cctactgaaa atctagaaga cttttataac aaagagggat atcgagatgg agaatttaaa
      301 aaagatgata aaggtacttg gctaattaga tctgaaatag ttaaacagcc aaagggcaaa
      361 gtgatgaaaa caagaggtat gcaattatat attaatagaa ataccaaaac agccaaaggt
      421 ttctttgttt tgaaagaaat aagtgaaaat aataatcgtg taaataaaga taaggaggaa
      481 aaatacgaag tgaaaatggt aggaaataaa attattccta ctgaacaaat taatgacgag
      541 aaaataaaaa aagaaattga aaacttcaag ttttttgtgc aatatggaaa gtttaaaaat
      601 ttcgaaaaat acaaaaatgg tgagttttca tataatccag aagcaccaat ttattcggct
      661 aaatatcaat tacaaaacga cgattacaat gtaaggcaac tacgtaaaaa atatgacatt
      721 tcaacaaaag aagcgcctaa gttactttta aaaggtggag gggatttaaa aggttcctca
      781 gttggtcaaa aagatattga atttacattt gttgaaagaa aaggtgagaa tatttatttt
      841 aatgatagtg ttgaatttat accaagtgag gataaataaa tatgagtaaa ccagaatatc
      901 aactaaaccc aggtaaaata aatgcaaata cagaagaaac atctgctata tcctatttta
      961 gttatcaaat tgaaaatgca aatattagtg gtttggataa agtaaaaatg tatcagcaaa
     1021 ttgatatact aaaagataga tacaaattcc cttctaatct tgaatatgtt gatagttaca
     1081 ctgattctct tactggagta acaacttctg cttttttaaa taaagataca ggcaaagtaa
     1141 ctctcgggat gactgggact aatttacaag acgaagcctt taaaaagtta aaagaaggtg
     1201 aattttcaag acaaaatttt accaatgctt tggaaacagt taaagatgga tatgcagatc
     1261 ttaaaatatt atattctcct gcatctgatc aaaactatag atatgcgaat acacaagaat
     1321 ttataaataa aataaaaagt aagtatgaca ttgattttat tactggtcat tcactaggtg
     1381 gaagagatgc agtagttcta ggaatgagta atggtattcc gaacattgtg gtttataatc
     1441 cagctcctat ttctataact agtttgaatc ctaattcccc agatggaaaa cgattgttag
     1501 agttatataa aaattataaa ggtaatatta ctaggtttgt tgcagaaaat gatgcattga
     1561 cagaaaatct gaagaaatat aagcattatg tttttttcgg taatgataaa gtctttaaaa
     1621 atggtaaagg tcatggaatg gaaggctttc tgaccaaaga agaacaaaaa gctataaaaa
     1681 aagaactaaa aaaactacaa ggttatgcag aagaaaataa taagtcattt gtaaagcatt
     1741 caaataatgc tatctctaaa ttagctagta tagaattact tagagcaaat atgatgacta
     1801 caaatggagg aggattatct tcttcgcagc aaaaagtttt agaaagttta acagctttaa
     1861 caattgcgca gtcattcagt caactgatag acgatgaaat taatcaaatc aaaaaaatgt
     1921 ataatgaaaa gaaaaagaaa tttggaaaaa attgggaaga cgctcaaaaa gctggaaaag
     1981 ctgtaggtga agatttgagt gtaaatggag ttctcaatgc tttagatgaa ggtcaagtga
     2041 atgaaagtag tatggtaaga gaacctgaac aaatgatatc tgcaaaagaa agacaacttt
     2101 caacgatagg gtcttctgta tcaaattata ttatgagagt tagactcagt attaatgaaa
     2161 tcgttgataa agatcaagtg cttgcatcac aaataggtgg attactatga tgtttaatca
     2221 aattaataat aaaaatgaat tagaagaatc atatgaatct gagaaaaaac gtatagagaa
     2281 tgaactgcaa aatttaaatg aacttaggca tagaactcga aaagaaaatg aacgtagtta
     2341 tgatgttttt caatatttga agcacgaaat gaattatagt gaagatgccc aaaggaaaat
     2401 gacgagaaat atagaagcgt atgagcaaga aatcaatgag ataattagaa agcaagaatg
     2461 gaaattagaa gaatataaag aagacttaaa aaagtcttat gaaaagcatt tagataaact
     2521 aagtgactga taatttggag gagattaaat gactctaata gaaccagata tgaacttaag
     2581 aatgccagat ataagtacta cagtagaaac acttaatctc atatctaaaa tggaagcgca
     2641 aaaagaaaat attcgcacag ttattgcacc tgaacataag cataaatata aagatattga
     2701 aaatgggtta aaaggtgaag aaaaagtatt aattgaacaa atggcgcagc attgcgaagc
     2761 ttttaaagct aattttaaag gcgcagctca aggagattgg gttaaaagtg ctatgtctga
     2821 gattgacagc attaaggatg acctgaaaaa aattaatagc taaactacat atcatattaa
     2881 taaataaagc tcccttcaaa attttcattt tttcaatgtc tactttggag ggagcgcttc
     2941 agtatattca caactgacac taggccgctt ctttttaatt tattttattc ccatgtaaag
     3001 taattcgaac cgaatggaca taccacactc acaattgtaa aaacacaact taaattactg
     3061 tcccgcatta aacatatcca cattccaaac agcaacaaca aaatcctcaa gtaccaaaag
     3121 ctatgaccac tttatcaaaa tcaacgcaag tcaacatcga ccgaactact tgccattaat
     3181 catcctcacc ttacaaacca taaagcgctg cagttaagta ttgaatggca tagccaattg
     3241 aaatgaaaat agcaatcgtt ataatagcac tgccaatggt atataacgta tgtttcgtag
     3301 ataatccttc agtctttgaa ccaaaaacac tgagtagaat aaatgcaata ccaatgacat
     3361 ataaaactaa aatgacaaat agcatgttta tacacctcga ttctctttcg tgagatatat
     3421 ctttgtctcc attataaagt aaagcgctca ataatttaat taagtatact atttttttat
     3481 tatcgaagat tgcgaaagat gaaacgctcc tatataatgt aaaacggaat tattcctatt
     3541 tacaaataaa ttgaagtgag ggtatatata tggctaaaat tccagttacg gtattaagtg
     3601 gttatttagg ctcggggaaa acaacattgt taaatcatat tttacaaaat cgtgaaggtc
     3661 gacgtatcgc ggtaattgta aatgatatga gtgaagttaa tatcgataaa gatcttgtcg
     3721 cagatggtgg gggactatcg cgtacagatg aaaaattagt cgaactctct aatggttgta
     3781 tctgttgtac acttagagat gatttattaa aagaagttga gcgtttagtg aaaaaaggtg
     3841 gcatcgatca aattgttatt gagtcaacag ggatttcaga gccagtacct gttgcacaaa
     3901 ctttctcata tattgatgat gaacttggca ttgatcttac agcgatttgc cgtttagata
     3961 caatggttac agttgttgat gctaaccgct tcgtacatga catcaactca gaagatttat
     4021 tgatggatcg tgatcaaagc gttgatgaaa cagatgaacg ttcgattgct gatttattaa
     4081 ttgaccaagt tgaattttgt gatgtattga ttattaataa aattgattta attagtgaag
     4141 aagaacttgc aaagttagaa aaagtgttaa gcgcattgca acctactgct aaaattatta
     4201 agacaacaaa ttctgaagta gatttaaaag aagtcttaaa tacgcagcgt tttgattttg
     4261 aaaaagcgag cgagtcagca ggatggatca aagaacttga gtctggtggg catgcatcgc
     4321 atacacctga aacagaagaa tatggtatat catcgtttgt atataaacgt cgacttcctt
     4381 tccatgctaa aaggttcaat gattggttag aaagcatgcc aaataatgtc gttcgatcaa
     4441 aaggtatcat atggctagca caatacaatc acgtagcatg tttattatct caagcagggt
     4501 catcttgcaa tattcatcca gttacatatt gggtggctag tatgtctgaa gcgcaacaaa
     4561 cacaaatatt agcagaacgt caagatgtcg cagctgaatg ggatccagaa tatggcgatc
     4621 gtcatacaca atttgtcatt attggtacag aattagatga agaaaaatta acaaaagaac
     4681 tcgacgcatg cttagtcaat gcgcaagaaa ttgatgcaga ttggcaacaa tttgaagatc
     4741 catatcaatg gcaaattaga ccagcacgat aagttgaatg aagtataatc atttttaaat
     4801 tgtggcttaa ttgtttgcaa tttatgacaa ttaataaaaa gttaaggtgt tcttaggatc
     4861 tttttgtttt caatctattt aaagtgtgat agttttgata aagcagaaat ttgcatttta
     4921 tccatagggc taggacatgt atgtgtctta gtccttttta tgtttgcttt gatgaaaaat
     4981 ggcaaaacca tcgtaccaac tgttaattga aggtacaatc attgtttaac agtcataaaa
     5041 atgagtgaag taaatgcagc tatcaatgta ctattaacac ttacggtcac acgagtgttt
     5101 cagtctaaac gtgcaacgca attccgcacg tggccaatat atcttgcgct agatcatgaa
     5161 aatcattaag atttcgcacg ccaaatcatg aatgtctcag acctcgagta tcaaacataa
     5221 aacacgccac aaaaatataa cgcgtcagac tacaaagatc acgaatattc cgtacgtccc
     5281 acgaagaaca tcctatacaa aaaaaccaag accatttatt tgcaacaaac taacaagact
     5341 aacctcacat caataaatca acacaaagca aagccaccat ccctattggc atagtggcct
     5401 gagaatttta agtattcaat tcgcttaaat tattttgcga aaatgtcgat aattgctttg
     5461 atgattttaa tgatagtacc tacaatagcc atcgttttgt cctcctgtat gttgatttaa
     5521 catagtgatg agttgttgat cgttaatgtt tgagattagt tgttacctaa aaatttacca
     5581 agtaaatctt taaagaattt gaataatttt gctacgaatt ccatgtgaat ggcccccttc
     5641 aaataagatg ttcatatcta tgagattttt ataacttact taccagtgaa tttctcaatt
     5701 aatcctttaa tgaatttaat gattcctgca atgataccca tgtgaaagac ctcctttgtt
     5761 tgttatgaaa tcttatttac cagtgaattg ttcgattaag cttttgataa ctttaatgat
     5821 gccagcgatg atacccatta agattacctc ctttgcttat gagttaactt cattgtacat
     5881 agttatcttg tgcgtaattg atttttttcc taacttgaat tgttatgatc ataactgcga
     5941 attttttatt ttaaaaggac gggaaatact gcctaactgt acaggtgcat ttacaaaatc
     6001 tttacaatca ttggtttaat agaggttaag ctgatgaatt tgacatgaag cagaaattta
     6061 attatatttt atgttataag tttaaaacga ataacacgtt aggtctcgtc taggcaaagc
     6121 atatttatga agatgaattg aacagacaga agtaacattt tatatacata tgtgcatggg
     6181 agcacgctta aagagaacac tgcaacaagg tatatcaacg taccgtgatg cagtgtctag
     6241 ttaaactgaa acctgcaata gtaaattttc tgccattatg tacagaatct actattgtag
     6301 gtttttattt ttatacataa gcaattatgc gagagattag aaataaggag gttatgcagt
     6361 gttaagtttt caattgctat tttcactgtt tgttattgcg cttatcattg cattgataag
     6421 tggcttgttg tttttagcac cagttatgct aatgagatat attaaattac atttatacat
     6481 actagtcatg ccagtattat ttgcagtcat tggctttttc ggtattcatg gtcaacatgt
     6541 cttaggtcca tttaaaatag atcgtttatc ttggttatta gctggctttg taatggcgct
     6601 tggctttatt attcaaaagt tttcaatgcg atatttacta ggtgatcatc attatagaca
     6661 ttactttcca ttgttcactg cgattacgtc gtttgcatct ttagcatgga tgtctgaaga
     6721 cttaagactg atggcactct gctggggtat gacattatta tgtttaacat tgctgatgaa
     6781 cgttaatcgt ttttggaaag tgccacgtga gtctgcgaaa ttatcaagta tgacattttt
     6841 atgtggttgg cttgcattcg ttggagcaat tgtaactatt tatattgcga ctggcgagtg
     6901 gcgcgtgcct caacatatgt ctaattcgac atggtcattg ttgacgaatg tactacttgt
     6961 attagctgtc atgataccgg cagcacaatt tccttttcat cgatggttga ttgaatctgt
     7021 aacggcacca acgccagtat cggcaattat gcatgcagga attgtgaatg caggtggtgt
     7081 tattctaact cgttttgcgc cgatatttga taatggattt gcgttatcat tattacttat
     7141 cctttctagt atttctgtat tgttaggatc gggtattagc ttagttcaag ttgactataa
     7201 acgccaatta gtgggctcta cgatgagtca aatgggcttt atgttagttc aatgtgcatt
     7261 gggtgtatat tcagcagcga ttattcattt aatattgcac ggtattttta aagcaacatt
     7321 atttttacaa tcaggttcta tcgtgaagcg attcaatatt ccaaaacaag catctgctaa
     7381 agacgcttat ggctggattg tcatgggacg tgtattagct attatcgtgg catttctatt
     7441 ttggatgagt agtgacagaa gtgcatatga agtgttaagt gcactcattc tagcatggtc
     7501 attacttgta tcttggaatc agatggtagc ctttagtaaa gggcgcatgg cacgtcttgt
     7561 tggcatgatt ttgattgcaa ttgtgacatt tatctacatc atcacacata attattttta
     7621 cgatgtattg cgaaatataa caacacatgc gacaacaccg cctacagtga gtgtcatcat
     7681 tagtgttgtc attttaatct ttggtagttt attaagtatt tgggtggcgc gtcatcgata
     7741 ctctaagggt tttgcggtat tgtacgtgtg gttagttaat ctaggtgaag cacgctcgaa
     7801 agcgatagaa agtcatccga attatttgaa gaagtattta taggaggtga aaggtatgac
     7861 aacacagtta aatatcaatt cagtcattga aaatgcgaaa cgtgttatta caccattatc
     7921 accaatttcg atttttgcag cacgaaatcc atgggaagga ttagaagcgg atacgtttga
     7981 agatgttgca aaatggttac gtgatgttcg agatgtggat atttttccaa ataaagcatt
     8041 aatagaaagt gctgtggcac gtggtgaatt agacgaaagt gtctttaatc aacttgttac
     8101 tgatatgtta cttgaacatc actacaatat cccgcaacac tacatcaatc tttatattga
     8161 taacattaaa acattaaaag acgtacctac atcatatatg aatcattcaa atgttgatgt
     8221 tgttgctgat ctactattag aaaaatcaaa acgtgatatg gctgaatcat atcatcacta
     8281 tgatgtacgt ccgatgagtg atgcaataat agatgaacaa ggtgagccac ttagcgaaca
     8341 agtgaatcgt caaatgatta aatggacgaa actttatatc gatcaatttc tatcgagttg
     8401 gacaatgccg aagcgagagc aaagttttta ccatgcatgg ttgcatttag cgcaacatga
     8461 ccatagtttt actaaagcac agcgccaagt gattaaaggc ttacccaatg atcctgaaat
     8521 gacgatagag tcagtattaa ctcatttttc aatagcgcag gaagactacc aagcttgtgt
     8581 tgaaggacat cttttggcgt taccgggttg ggcaggtatg ttgtattacc gttcacaaca
     8641 gcatcacttt gaacaacatt tgttaacgga ttatttggcc attcggttag ttgtcgaaca
     8701 attgctagtt ggtgatgagt ttaagtcagt cactaaagat tgtgaaagta gatcggaaaa
     8761 ttggtttaag caaactgttg catcatggtg ttactacagt gatatgccta gcgatgtatt
     8821 actacaacat gacgtcaatg aaattcaaac gtttattcat tttgcagcaa ctatgaataa
     8881 aaatgtattt aaaaatttat ggctaattgc ctgggaaatg acatacgaat ctcagttaaa
     8941 acaaaaaatt aaagcaggtc atgagagtgt ggcgggcgca ttagatgtaa accaagtaaa
     9001 tgtcttagaa aatgataacg ctaatcagcc acattcagta ttgttaaatg acacacaagc
     9061 agttgatgaa aataatagcg agctaaatca gatgggcaca tcaacgaaag cgcaaattgc
     9121 attttgtata gatgttcgtt cagaaccatt tcgtagacat atcgaagcgg cagggccctt
     9181 tgaaacgatt ggtattgcag gtttctttgg attacctatt caaaaagatg ccgtagacga
     9241 acaattcaaa catgattcat tacctgtcat ggtaccgccg gcatatcgca ttaaagaatt
     9301 tgcagaccgc tacgatatga atgtttatcg acaacagcaa cagacaatgt catcgatgtt
     9361 ttacacattt aaattgatga aaaataatgt catgcctagt ctgttattgc ctgaattaag
     9421 tgggccattt ttaagcttga gtaccattgt caattcgatt atgcctagaa aaagtcgcgc
     9481 gtctttacaa aaaataaaac aaaaatggtt gaaaaagcct gaaacaaagt taacgattga
     9541 tcgtgagttt gaccgaacat cagacttacc tgttggattt actgagcaag agcaaattga
     9601 tttcgcgtta caagcgttga aattgatgga tttaaccgaa gcatttgcgc cgttcgttgt
     9661 gttagcaggt catgctagtc attctcataa taatccacat catgcatcac ttgaatgtgg
     9721 ggcttgtggt ggcgcatcaa gcggttttaa tgctaagtta ttagcgatga tatgtaatcg
     9781 tccaaatgtc agacaaggat taaaacaagc aggcgtagta attccggaga caactgtttt
     9841 tgcggcagca gaacatcata cgtctactga tacgttggca tgggtatatg tgccagacac
     9901 attatcagct ttagctctag atgcatatga atcattgaat gacgcgatgc cgatgatttc
     9961 tgaacacgcg aatcgcgaac gtttggacaa actgccaacg attggtcgtg tgaatcatcc
    10021 agtggaagaa gcgcagcggt ttgcgagtga ttggagtgag gtacgtccag aatggggctt
    10081 ggctaaaaat gcatcattta taattggacg acgccaatta acaaaaggca ttgatttaga
    10141 agggcggaca tttttacaca attatgattg gcgtaaagat aaagatggca cattgttaaa
    10201 taccatcatt tctggtccgg cacttgtggc acaatggatt aatttacaat attatgcgtc
    10261 gacagttgcg ccgcattttt acggaagtgg gaataaagcg acacaaaccg tcacgtcagg
    10321 tgttggtgtc atgcaaggta atgcgagtga tctgatgtat ggcttatcat ggcaatctgt
    10381 tatggctgct gatcgaacga tgtatcattc gccaattcgt ttgcttgtcg ttgttcaggc
    10441 acccgactat gttgtagcaa gactactcgc gaataatgac catttcgcta ggaaggtgtc
    10501 taatcattgg ctgcgtttaa tgagcgttaa tgaggaaggg cgttttaaaa gttggattta
    10561 acgatgatat gttgcgatgc taagtaacta atatatcatc atatctacaa tgttctagct
    10621 tcttaaaata tactaaaaat gataaactta atgtaatagt tgatgtcgtg ataacgatac
    10681 aaagatgaag taatcaattt taatattatt ggagttagtg atatgaaaag aacgaaaggt
    10741 gaaatcgaag ctgaaatcag taaagccatt acgcaatggg aaaaagattt ccttggcaga
    10801 ggttccttgt cagttaaatc agacatttta agagatatgg tgattattag tttacaaggt
    10861 atcttaacgc cagcagaata tcgtgtgtgt agtacgaatg aaggattatt aaatattaaa
    10921 cgaacacgtt ctgaattagt tgaatccggt gagcaagatt tgaatgatat cattcttaaa
    10981 attacaggta tcaaagtgat gagcttccac agtgatttaa gtacagttac aggtgaacgt
    11041 attatcgtat tcaaacttga ggataatttg gaaaagcata tttaagaagg ttaggtatgc
    11101 ttctgctggc ttattaaagc tatagaagta tgcttggtta aacagcgcaa cgccatatat
    11161 ttgggtgtgc tgtttttcta ttatatgatg tgtttattac gtggatgaat gcgtgggtct
    11221 gaatatagcc cgtgaaaggc gtatgtcata aaatttgcga atggtgacaa gtcaacgcaa
    11281 gcatatttgt tgcgagacat gcccactatc cttttacata aagagtatat gtgtgacgta
    11341 tgcatataat cgataaagta tacctaaaaa attaggtata atgaccattg ttgcaataag
    11401 tttttcggcc ttgaaaccga taacgcatag tataatagga ataaaatata atacaaatat
    11461 ccagtaaatc aaatttgaaa acgatggatg aataggggta aagaatctaa ctaatgtaat
    11521 gattaacgct atatagacaa aaataactct attgatacgc cttaccccct ctgtataata
    11581 actataaatc gtactaaatt gaaaatagca aaatattgtt atttcaatta tacaatgttt
    11641 tatttgcaat atacataaca atatgaactt tataattttg atattgtcgg taaagtgaaa
    11701 tttgtggcac attgatatta tggataacat ttgtaaaatg aaaatgatga tacgaggaga
    11761 ttgtaagatg atagataaaa aattaacatc accgaaaatg acagtgcctt tatttttaat
    11821 cgcgctgatt gtatttatag gtatgtttta cagtgtagtg acaaatcaag aatggcttaa
    11881 aaatatagat atgggatcat taacatggtt tacagattat ttcggtgagc cacaacgtca
    11941 gtatgttaac aatttattta attactatat gacgtttagt gcggaaattg gagatgtcaa
    12001 aggtgtcgtg ttgatttcca ttatcatcac aatcatactg tttattaaac agaggcattt
    12061 agcggtttgg tttgtgacat atttggtttc aggcgtcatc atgaacaaat taattaaaga
    12121 tactgtttta cgtccaagac catataatca tttagccgtt gatacaggct tttcatttcc
    12181 aagtggacat tccaacgcca gcacattatt atatttcgcc ttaatgatca taattatttc
    12241 acttgctgct aagacaataa caaaagtgtt gagtgcgcta gttatgggaa tattatggct
    12301 tagcatatta ttttgtcgcc tttattttca tgcgcattac ttttcagatg tcattggcgg
    12361 cacgtcacta gcaatcattt gggtagcgtt attcttaatg gtatacccat actttattaa
    12421 tcatcgacga caacgcgttt agcaaacatc tttagacata gtggaggtat atagaaaaat
    12481 gagaattaaa acaccgagtc catcgtattt aaaaggcaca aatggacatg cgatattatt
    12541 attacattca tttacaggta caaatcggga tgtgaagcat cttgcagctg agttaaatgc
    12601 acaaggattt agttgttatg caccgaatta tccaggtcat ggcttactgt tgaaagattt
    12661 catgacatat aatgtagatg attggtggga agaagttgag aaagcttacc aatttttagt
    12721 caatgaaggt tatgaatcta tcagtgcaac gggtgtgtct ttaggtggat taatgacatt
    12781 aaaattggcg caacactatc ctttgaaacg tatcgctgtc atgtcagcac caaaggaaaa
    12841 gagtgacgat ggcttaatag aacatttagt ttattatagt caacgcatgt cgaatatttt
    12901 aaatttagat cagcaagcat cgagtgcgca attagcagca attgatgatt atgaaggtga
    12961 aattacgaag tttcaacatt ttattgatga tatcatgaca aatttaaatg ttattaaaat
    13021 gccagctaat atattatttg gtggtaaaga tgcgccatcc tatgaaacaa gtgcacattt
    13081 tatttatgaa catttaggat cagtagacaa agaattaaat ggtctgaagg attcgcatca
    13141 tttaatgacg catggagaag gcagagatat tttagaagaa aatgttattc gctttttcaa
    13201 tgctttaaca taattgaaga ttttaaaaat taacacttga gtgacgatgt tattatattt
    13261 gtcatttgag tgttttttgt ggcgtgatag tatagggaac ttagctaaag ttaatttgta
    13321 gtgtgatgtc gtggaaattt tatgggcatt gtacctgaag ttgttatgtg gcgagatgtt
    13381 ggggatatag atggtgttgt atgtggcgag atattggcgt acgtatacag cgttgaacca
    13441 gaaaatttaa tgtagcgtaa tgacaacaca ttacaagagt tgattatatt atgataaatc
    13501 caggtgctac cgtactaatg aaacaaattt gatgcaggtg tattgcatgt tatttacata
    13561 catgttagag tgttacttaa acacgtagac gctttaacgc tttaaaggag atgcgaatga
    13621 tgaaaagaca acaatcacaa tggaagtcat caactggatt tattttagct agtgcgggtt
    13681 ctgcaatcgg tcttggtgcc atgtggaaat tcccatatat ggcagggatt tatggcggcg
    13741 gtgcctttct agctatgttc ttaatattca ccatttttgt tgggttgcca ttactcatta
    13801 tggaattcac tgttgggaaa atgggacgga catatacaac acaaatatat agtaaattaa
    13861 ctggtaaaaa atggctcaat atcattggct ggaacggtaa tttggcagtg tttattttat
    13921 ttggcttcta tagtgttatc ggtggttgga ttgtcattta catcggacaa gtattatggc
    13981 aattagttat atttcaacgc atcaatcatc tccaagaaat gaattttgaa gcggtaatat
    14041 caaatccttg gttaactgtt ctagggcaag gtatattcat attcgctacg atgattattg
    14101 tcatgttagg tgttgaaaaa ggattagaaa aggcatcgaa agttatgatg ccattgctgt
    14161 ttgtcttttt aatcatcatt gtgattaagt ctttaacatt agatggtgcc ttagaaggtg
    14221 tgaaatttat tttacaacca agagtatcag agattactgc tgacggtatc ttgtttgcgc
    14281 taggccaatc attctttacg ttatcattag gaactacagg tatgattact tatgcgagtt
    14341 atgcctctaa agacatgacg attaagtcat cagctatttc tatcgttgtt atgaatatct
    14401 ttgtatctgt attggcaggt ctagctatat ttccggcgtt acatagtttt ggctatgaac
    14461 cacaagaagg gcctggatta ttatttaaag tactgccaat ggtctttagt caaatgcatc
    14521 taggcacatt attctatttg ggattcttag tgctgttctt atttgcggct ttaacgtcat
    14581 ctatttcttt attagaatta aatgtttcta acttcacgaa gaatgacaat acaaaacgta
    14641 aaaaagtcgc agtgatcggt agtattttag tatttatcat tagtattcca gcaaccttat
    14701 cttttggtat cttaaaagat gtaagattcg gtgcgggaac gatttttgat aatatggatt
    14761 tcatcgtttc gaatgtattg atgccattag gcgcattagg tactacgctt gtcgtaggac
    14821 aattattaga taaaaaatta ttacaacaat attttggtaa agatcgattt agattattca
    14881 gtggttggta ttacttaatt aagtatgcga tgcctgtcgt tattatctta gtctttatcg
    14941 tgcaattatt tagttaatat atagaagcat ctgacacaca agtatttgtg ttgatcaggt
    15001 gtttttttgt ttggaattga aaagttagta ctggttctga attaatcgaa acttagataa
    15061 ttgcaaaata acaaaatgtc gtttataatt cttatctgaa aagtaagaat taaaattgag
    15121 atatgaagaa aggtttcatt catttgatcg gcatgtcaga tgagggtgca tctatgatta
    15181 cttatgattt aattggcaat acaccattag tactgttaga acattatagt gatgataaag
    15241 ttaaaattta tgccaagctt gaacaatgga atcctggagg cagtgttaaa gacagactcg
    15301 ggaaatattt agtagagaag gcaattcaag aagggcgtgt gcgtgcaggt caaactattg
    15361 ttgaagcgac tgctggtaat acaggcatag ggttagctat tgcagcgaat agacatcatt
    15421 tgaaatgtaa gatctttgcg ccgtatggtt tttcagaaga aaagattaat attatgatag
    15481 cgcttggtgc agatgtttca aggacgagtc agtctgaagg tatgcatggg gcacaattag
    15541 ctgcacgttc ctatgctgaa aaatatggtg ccgtttatat gaatcaattt gaatccgaac
    15601 ataatccgga tacatatttt catacattgg gacccgaatt gacttcagca ttacagcaaa
    15661 ttgattattt tgtggctggt attggctctg gcggtacatt tacaggtacc gcacgttatt
    15721 taaagcaaca tcacgtgcaa tgttatgccg ttgagccaga agggtccgtg ttaaatggag
    15781 ggccagctca tgcacatgac gctgaaggta tcggttctga gaaatggccg atatttttag
    15841 agagacgtct tgtagatggg atatttacga ttaaagatca agatgccttt cgaaatgtca
    15901 aaagtttggc tataaatgaa gggttgttag taggcagttc ttcaggtgca gcattacaag
    15961 gtgcattgaa tttaaaagcg caattatctg aaggtacgat tgttgtcgta tttccagatg
    16021 gtagcgatcg atatatgtct aagcaaatat ttaattatga ggagaatgat tatgaataag
    16081 aaaactaaat taattcacgg tggacacaca acggacgact atacaggtgc agttactaca
    16141 ccaatttatc aaacaagtac atatttacaa gatgatattg gtgatttacg tcaaggatat
    16201 gaatattctc gtactgcgaa tccgacaaga agttctgtag aaagcgttat tgcggcatta
    16261 gaaaatggca aacatggctt tgcatttagt tcaggtgttg cagcaatcag tgcagttgtt
    16321 atgctgttgg acaaaggtga tcatattatt ttaaattcag atgtatacgg cggtacttat
    16381 cgcgcattga caaaagtatt tacacgattt ggcattgaag tggattttgt agatacaacg
    16441 catacagatt caattgtaca agcgatacgc ccaacaacaa agatgttgtt tattgaaaca
    16501 ccttctaatc cattattacg tgttactgac attaaaaagt ctgctgaaat tgcgaaagaa
    16561 cacggtttga tttcagtcgt tgataacaca tttatgacac cttattatca gaatccatta
    16621 gatttaggta tcgatattgt tttacattct gcaacgaaat atttaggtgg acatagtgat
    16681 gtcgttgctg gtttagttgc aacatcggat gacaagcttg cagaacgttt agcatttatt
    16741 tcaaattcaa caggtggcat tttaggacct caagatagct atttacttgt gaggggtatt
    16801 aaaacattag gtttacgtat ggaacaaatt aatcgcagtg ttattgaaat tattaaaatg
    16861 ttacaagcac atccagctgt gcaacaagtg ttccatccaa gtattgaaag tcatttaaat
    16921 catgatgtcc atatggctca agctgatggc catacaggtg tgattgcatt tgaagtgaaa
    16981 gatacagaaa gtgccaaaca attgattaaa gcaacatcgt attacacatt agctgaaagt
    17041 ttaggtgcag tggaaagttt aatttcagta cctgcactga tgacacatgc atccattcca
    17101 gcagatattc gagctaaaga aggtattaca gacggacttg taagaatttc tgtaggtatt
    17161 gaagatactg aagatttagt ggatgattta aaacaagcac tagatacgtt ataaataata
    17221 gcagcactgg catatatttt gagttcaacg tttgttgttc ttgaatatgc tagtgctttt
    17281 ttgtgcgatg ttaatatttc gtaattgcgt tgtggaggat tgctggtcat ttttattcat
    17341 ttaattcgat aaatgtcata gtagcctcac aattcatcat tgtcacatga attgcttatt
    17401 ttttaatttt ttagaaaatt cggcaaattg tattgacgat atatgcaatt ttacatataa
    17461 tgctcttatt cctagtggat taataagaat ttgtaggagg ggcgatgatg attgagtttc
    17521 gacaggtaag taagaccttt aataaaaaga agcaaaaaat acatgcttta aaagatgtat
    17581 catttaaggt caatcgcaat gatatttttg gtgtgattgg atacagtggt gctggaaaaa
    17641 gtacgttggt aagactcgtg aatcatcttg aagctgcctc aagtggtcaa gtgcttgtag
    17701 atggacatga tattacgaat tatagtgaaa aaggcatgcg agaaataaaa aaagatatcg
    17761 gtatgatatt tcagcatttc aatttattaa attcagctac cgtatttaaa aatgtagcaa
    17821 tgccactcat tttaagtaag aaaagcaaaa cagaaattaa gcaacgagta acggaaatgc
    17881 ttgaatttgt aggattgagt gataaaaaag accaatttcc tgatgaatta tctggtgggc
    17941 agaagcaaag ggtggctatt gcaagagcgc ttgttactaa tccgaaaata ctcctatgcg
    18001 atgaagcaac aagcgcattg gatccagcaa cgactgcttc gatattgacg ttattaaaga
    18061 atgtcaatca aacctttggc attacaatta tgatgattac acatgaaatg cgcgttatta
    18121 aagacatttg taatcgtgtt gctgtaatgg aaaaggggca agtggttgaa acaggaactg
    18181 ttaaagaggt gtttagtcat cctaaaacga cgattgctca aaattttgtg tctacagtta
    18241 tacagactga gccaagtaca tcattgattc gtcgattgaa tgacgaacaa gttggcgatt
    18301 ttaaagatta taaaatcttc gtcgaggaaa ctcaggtgac acaaccgatt ataaatgact
    18361 tgattcaaat ttgtggcaga gaggttaaaa ttttattttc atctatgtca gaaatacaag
    18421 gtaacaccgt atgttatatg tggcttcgat ttaatatgga tcaacaattt gatgacacgg
    18481 caataaatca atatttcaaa gagaaaaata ttcaatttga ggaggtgcat taacatatgt
    18541 ttggttctga tttagacagt gcacagttat tacaagcatt gtacgaaacg ctatatatgg
    18601 tatctattgc tttattttta ggagcagtga ttggtattcc attaggtgtc ttattggtaa
    18661 ttactcgaaa acaaggcata tggcccaata tagtgataca tcaagtttta aatcctttaa
    18721 tcaatatttt aaggtcacta ccatttatta ttttgttaat tgcgattgtg ccattcacaa
    18781 aattagtagt aggtacttca attggtacga ctgctgccat cgtgccttta acagtatatg
    18841 tggcacctta cattgcaaga cttgttgaaa actcattatt ggaagtagac gaggggatta
    18901 ttgaagcggc gaaagcgatg ggcgcttcac cactacaaat cattagatat tttttaatcc
    18961 ctgaagcgtt aggttcatta gtattagcaa ttaccactgc gattattgga cttattggaa
    19021 gtacggcgat ggcaggagct gttggcggtg gtggtatagg agacttagct ttagtgtatg
    19081 gttatcaaag atttgatacg acggtcatta ttattaccgt tattgtatta gtcattattg
    19141 tccaagtgat tcaaacgcta gggaatgttc tagctagatt catacgtaga cattaatggt
    19201 atatagtgaa gattttgaaa ggaattgata gaatgaaaag attgattggg ttagttatcg
    19261 tagcacttgt attattagca gcgtgtggta gtaacaatga taaaaaagta acaattggtg
    19321 tcgcatcaaa tgatactaag gcttgggaga aggttaaaga attagctaaa aaagatgata
    19381 ttgatgtgga gattaagcac ttttccgatt acaatttacc gaataaagca ttaaacgatg
    19441 gtgatattga tatgaatgca ttccaacatt ttgcattttt agatcagtat aagaaggcac
    19501 ataaaggaac aaagatttca gcattaagta caacagtttt agcaccgttg ggcatttact
    19561 cagataaaat taaagatgtc aaaaaggtta aagatggtgc taaagttgtc attccaaatg
    19621 atgtatcaaa ccaagcacgt gcacttaaac tattagaagc agctggttta ataaaactga
    19681 aaaaagattt cggtttggca ggcacggtga aagatataac gtcaaatcca aaacatttaa
    19741 aaattactgc agtagatgca caacaaactg cgcgtgcttt atctgatgtc gatattgcag
    19801 ttattaataa tggtgtagca actaaagcgg gtaaagatcc taaaaatgat ccgatatttt
    19861 tagaaaaatc aaattcagat gctgtaaagc catatattaa tattgttgca gttaatgaca
    19921 aagacttgga taacaaaaca tatgctaaaa tcgtagaatt gtatcattca aaagaagctc
    19981 aaaaagcgtt gcaggaagat gtcaaagatg gagagaaacc tgttaattta tctaaagatg
    20041 agattaaggc aatagaaacg tcattagcaa aataaataat attgcgtcct acaagcaaag
    20101 ttcatgctta tgtttgtagg gcgttattgt ttgacaataa aattatttcc aatagagaaa
    20161 gggattgtaa tcattttata gtgaaatatt atgaaattgt aataatttag atattgtaaa
    20221 atctaataag ttgtaataat tttaaggggt aattataaaa tttgatgata cagtatatga
    20281 tttttttgta atcataatgt catcaaacat caacctatta tacataatta aatcgtataa
    20341 tgatgtagta ttcataaatt cggataaaaa gaatgttagg aaagttaagc aagaggagga
    20401 ttttaaagtg caaaaaaaag taattgcagc tattattggg acaagcgcga ttagcgctgt
    20461 tgcggcaact caagcaaatg cggctacaac tcacacagta aaaccgggtg aatcagtgtg
    20521 ggcaatttca aataagtatg ggatttcgat tgctaaatta aagtcattaa acaatttaac
    20581 atctaatcta attttcccaa accaagtact aaaagtatct ggctcaagta attctacgag
    20641 caatagtagc cgtccatcaa caaattcagg tggcggatca tactatacag tacaagcagg
    20701 tgactcattg tcattaatcg catctaaata tggtacaact taccaaaaca ttatgcgact
    20761 taatggttta aataatttct ttatttatcc aggtcaaaaa ttaaaagtat caggtactgc
    20821 aagctcaagt aattctgcga gcaatagtag ccgtccatca acgaattcaa gcggtggatc
    20881 atactacaca gtacaagcag gtgactcatt gtcattaatc gcttcaaaat atggtacaac
    20941 ttaccaaaaa attatgagct taaatggctt aaataatttc tttatatatc cgggtcaaaa
    21001 attaaaagta actggtaatg caacatcttc aaactcagca tctgcaacaa ctacgaatag
    21061 aggttacaat acaccagtat tcagtcatca aaacttatat acatggggtc aatgtacata
    21121 tcatgtattt aatcgtcgtg ctgaaattgg taaaggtatt agtacttatt ggtggaatgc
    21181 taataactgg gataacgcag cggcagcaga tggttacact atcgacaata gacctactgt
    21241 aggttctatc gctcaaacag atgtaggtta ctatggtcat gttatgtttg tagaacgtgt
    21301 aaataacgat ggtagtattt tagtttcaga aatgaactat tcagctgcac caggtatttt
    21361 aacttacaga acggtaccag cttaccaagt aaataattat agatatattc actaaagtct
    21421 tacgtatata aatatataat gaattcctat tacatttcac aagctgaaaa gttggattga
    21481 atcctaaact ttatggcggg gctatcaaag tcgtgattgt tgtaatagga atttttgtat
    21541 atgaaaaagg attggtcgaa tgaacttcat gttctatgtt cgaccaatcc ttttctgaat
    21601 tagtattcat gctttacttt gcgaatgctt gtaaataatc tagcaccgtt tgttattaaa
    21661 gtaacaactg ccactgcttt tttgaactta cgtggtgact taattgaaat gtaaaagtct
    21721 aacacggttc ctacagtgct taaacctaat gaaagatata ctaatttttt atttttagca
    21781 tgatatttat agccattgta gccgtcgact atgaaacctg cgacatttag taaacttgat
    21841 aaacgttgtg atttggaacg ttttgccata atattaattc cccctagtat cttctgttta
    21901 tatgaaaagt tgggtgcgct gaattgctaa cgttttgcgc tataactact catatatgat
    21961 aacataattg tacagtataa tttgaaaaat tgatttcaca aagttggggt gtcaaagatg
    22021 attaaatgtg tctgtttagt tgaagaaaca gctgataaaa tattgcttgt tcaagtaagg
    22081 aatcgcgaaa agtattattt cccaggtggt aaaatagaag aaggggaatc acaagtacaa
    22141 gcgctgttaa gagaagtaaa agaagaatta aatttaacat taacaatgga tgaaattgaa
    22201 tatatcggga caattgtagg tcctgcatat ccacaacagg atatgttaac tgagttaaat
    22261 ggatttcgcg cattaaccaa aatcgattgg gaaaacgtaa ctatcaataa tgaaattacg
    22321 gatatacgct ggatggataa agataatgat gcgttgattg cgcctgctgt caaagtttgg
    22381 attgaaacgt atggtggtaa acatgacaaa taatgacacc atcatgttac gacattatgt
    22441 cccacaagat tattcgatgt tagaagcttt tcaattaagt gaaagtgatt tgaagtttgt
    22501 taaaacgcca gaggaaaata ttacagctgc aatgtctgat aatgaaaggt atcccatcgt
    22561 tgtaatggat ggcaggcaat gtgtggcctt ttttacatta catcgtggaa aaggggtcgc
    22621 accatttagc gataaccaag atgcagtatt tttcaggtca tttagtgttg atcaacgtta
    22681 tcgtaataga ggaataggta aagtggtaat ggaaaaattg gcgtcattta tcacttcaac
    22741 atttcagggt attaatgaga ttgtgttaac ggttaatact gacaatccac atgccatggc
    22801 actttatcgc caacaaggat atcaatatat gggagatagt atgttcgtcg gaagacctgt
    22861 tcatattatg gcgttaacta taaaataaat taaatttaaa agcatcttta ctcatcgtcg
    22921 atcacaacaa ttaatgatga ataaaggtgc tttttgttat agatcaccgg acaatttact
    22981 atagtaaaaa gcgacctagt gaacaattga catatatcca caggtcgctt aacttaaatt
    23041 atattgctag ttgcgattaa ttgatagact catcattttt gcgctgtcga gatggtcttt
    23101 ttattaaaaa tgccgtaatc caagccgtaa tcggaatact gattgcaacg gcaataccgc
    23161 ctaaaataat agaaataaat tcttgagcaa atattttcga gtttataata tgaccaaatg
    23221 aatatttaag tttgaaaaac caaataaata aagcaagttg gccaccaaaa aaggcaaggt
    23281 aaatcgtgtt cgcagatgtc gctaaaattt ctctaccaac acgcatgcca gattggaata
    23341 attcgtattg cgtaagtgtt ggattcactt gatgcaattc ataaatgggt gaactaatgg
    23401 taatcgttaa atctatcaca gctgcaataa cagcaagaat aatagtgaac accataaatt
    23461 gaaccatatc aatgccaata ttcattgaat acacatatgt ttcatcttgt tgttcggttg
    23521 aaaagccttg tagatgaccg aagtagaccg ataaataaat gagtgtaatc aacaatattg
    23581 ttgtaacgat agtgctgata aatgcagctt gtgttttaac attgtaacta ttgagtacga
    23641 ataaattaca agcgccaata ataatgcaga aaaagaatgt gacgacataa atcggtacgc
    23701 caaaaataat caacacaata ctaataatta aaatagcgaa atttaaaaat agggttaaat
    23761 aagagatgaa tcccttttta cctccgaaaa ttatcatcag aaagaggagc aataacgcca
    23821 atataaatac agcattcatt gtttcgccct ccttaatgtt tcaaatattt ccataaacaa
    23881 tattgtgata ggaattgtaa gtacgatacc tataccgcct gttagtgcgc gcgcaatttc
    23941 taacgaccaa ttcatcgaaa tagtataagt cacagtattt gcatttttta aaaagattaa
    24001 aaacataggt agtgcaccag ataaatatga gaataataag atgttagtca ttgttcccat
    24061 aatatcttgg ccgatgtatc gcccagcaag cgcccatctc ctcattgaaa tgtgtggcgt
    24121 acgctgtaaa atttcatgca taccactagc aattgtaatt gcaacatcca taatagcgcc
    24181 aagtgaacct attaacactg aggctaggaa gatatctttc ggtggtaatg ataaaaagtt
    24241 catcgtttca tatttaatgc ctttaccatc tgtcatatat atgattaatt ctgttaaacc
    24301 tatactcaaa aaagttccga taattgtact ggctatggta atgagtgtac gcatatgcca
    24361 gcctgtaacg agcaataaag tgagtactgt tgaacagatc atggcaatgg tcatgagtaa
    24421 gaataaatta atattgctat gttgaatatg aatgtaaatt gcgattaata tggcaataga
    24481 attcaagatt aacgataaaa tcgattgcag tccgactttg cgaccaacca ataatacagt
    24541 taataagaac aaaccagtga tgataaccgt taaggtatca cgcttctttt ctataatata
    24601 agcatcactc ggcttgttag aaatatgtaa taatactttt tcgtgtgtgc gaaatgcctc
    24661 agaatctgct tgtgatttga cgtactgatg attaatcgtc gttgtttctc cagcaaattg
    24721 accatttaat attttgactt ttaattgatt tttatattta atatcacgat tattttgtgc
    24781 atcttttgta ggtgtcgaag aaacatgttt gacatctata atttgaccaa ttggtttgtt
    24841 gtaaaagttc tcattattga atgtaaataa aatagcacca atgaatgcga tgcagaacaa
    24901 acctaaaatt atattaaatg gctttgtaaa taaatttcta tatttcaaaa acaaaacccc
    24961 aattctatga atgaattaat atggtgatta tacaccctta attttttatt ttcaaagata
    25021 ttactgctaa gtgtaaaacg aaaatcatca ttgatagcat cgaattactt aatggaatgt
    25081 agacgtttta gtcattaatt gctgaataag tgttaataat atgccaatat cactctttgt
    25141 ataaggctcc tttgtaatag cacatatcgt tctttttaat tcagtatgat ctaattttat
    25201 atctatccat gatttagatt ctggtaaatg tatattttgt gatgaaatga tgtaaccttc
    25261 tttttgacga aggagatact gcgcaagtgg ttggctactg attgtgtata catctgattt
    25321 agtaatcttg cgcaattgtt tttttacagt ttcggcaaat ggtgccaagc aataaatatg
    25381 actatgctca aactgaatta atggtgggtg tgtcgccatc gtaattggat cgtctgaagg
    25441 cgcatataaa tgatagtgct cttcgaataa aggtagcata tgtaattgtt tgtgtttacg
    25501 tatttctggt gtaagttccg tgaaaccaat gtctatattc ccatttaata cgctatttat
    25561 aattgtgtca tgttctaata aattagggat gacatgtgta tcattttgta aatgaaacgt
    25621 ttggataagt ggtagtaaca tgtgggatac gtcactctca tcatagccaa tgtagatact
    25681 tttattttta gttaatccat ggctttgaaa ttgttcaatc gtgctatcta aatgttcaat
    25741 aatacgcaga gcttcattaa ataataattt tccttcagat gtgagcgtaa tattgcgtcc
    25801 ttgcttttta aataaagaca cattaagttc ttgttctaat aatgtaattt gacggcttat
    25861 cgctgattga gcaatgttta gttcaagtgc tgtttcggag atatgttctc ttttagcgac
    25921 ctcgataaaa tatcttaatt gtttaatttc catagcgata taggcacctc caaaaatgag
    25981 tgttttgtaa ctattatagc aatattattg ataaatgttc tattttttag atgaatatct
    26041 tctattttat atattgaaca gataaatttt ttagattata gtaattatca ttaataacta
    26101 atatcagaat attctaaaaa aggggtgtgc atcatgcaca atgagaaatt aattaaaggc
    26161 ttatatgact atcgtgagga acatgatgcg tgtggtattg gtttttatgc gaatatggat
    26221 aataaaaggt ctcacgacat cattgataaa tcgcttgaaa tgttgcgacg cttagatcat
    26281 aggggcgggg tcggcgcaga tggcatcact ggtgatggcg caggtattat gactgaaata
    26341 ccttttgcat ttttcaaaca acatgtaacg gactttgata tcccaggtga aggtgaatat
    26401 gccgtggggt tatttttttc caaagaacgc gttttaggtt ctgaacatga agcagttttt
    26461 aaaaaatatt ttgaaggcga agggttatca attcttggtt atcgtaatgt accagttaat
    26521 aaagatgcca ttgctaaaca tgtagcagat acgatgccag tcattcaaca agtgtttatt
    26581 gatattaggg acattgaaga tgttgaaaag catttgtttt tagcgagaaa acaattagag
    26641 ttttattcga ctcagtgcga tttagaattg tattttacga gcttatcacg caaaacaatt
    26701 gtatataaag gttggttacg atcagaccaa attaaaaaac tctatacaga tttatcggat
    26761 gatttatatc aatcaaagct agagttagtg cattcgagat ttagtacgaa tacattccca
    26821 agttggaaaa gggcacatcc taaccgtatg ttaatgcata atggtgagat taacacgatt
    26881 aaaggtaatg taaactggat gcgagcacgc caacataaat taatcgaaac attatttggc
    26941 gaggatcaac ataaagtgtt tcaaattgtc gatgaggatg gtagtgactc tgctattgta
    27001 gataatgcgc tagagttctt atcgttagcc atggagccag aaaaggcagc gatgttactc
    27061 atacctgaac cttggttata taatgaagcg aatgatgcaa atgtacgtgc gttttatgaa
    27121 ttttatagtt atttaatgga accgtgggat ggtcctacaa tgatttcgtt ctgtaacggt
    27181 gacaaacttg gcgcgcttac agatagaaat ggattacgtc caggtcgtta tacgattact
    27241 aaagataact ttattgtctt ttcatctgaa gtgggtgttg tggacgtacc tgaaagtaat
    27301 gttgctttta aaggtcaatt gaatcctgga aagttattgc ttgttgattt taaacagaat
    27361 aaagtcattg aaaataatga tttaaaaggt gcgattgctg gagaattacc atataaagcg
    27421 tggattgata accataaagt tgactttgat tttgaaaata tacaatatca agattcgcaa
    27481 tggaaagatg agacgttatt taaattacaa cgtcagtttg catacacgaa agaagagatt
    27541 cataagtata ttcaggaact tgtagaaggt aagaaggatc ctatcggtgc aatgggatat
    27601 gatgcgccaa ttgcagtgtt gaacgagcga ccagaatcac tatttaatta ctttaaacag
    27661 ctgtttgcac aagttacgaa tccaccaatt gatgcgtatc gtgaaaaaat cgtaacgagt
    27721 gaactttctt atttaggtgg cgaaggtaac ttactagcac ctgatgaaac ggttttagat
    27781 cgtattcaat tgaaaaggcc ggtattgaat gaatcacact tagcagcgat tgatcaggaa
    27841 cattttaaat taacttattt atcaacggta tatgaagggg acttggaaga tgcgttagaa
    27901 gcattaggcc gagaagcagt gaatgctgta aagcaaggcg ctcaaattct agtgttagat
    27961 gacagtggat tagttgatag caatggcttt gcaatgccga tgttactcgc aataagtcat
    28021 gtgcatcaat tacttattaa agcagattta cgtatgtcta caagtttagt cgctaaatct
    28081 ggtgagacac gagaagtgca tcatgttgct tgtttactcg catatggcgc gaatgcaatt
    28141 gtgccatacc tagcgcaacg tacagttgaa caactgacat tgacagaagg gttacaaggc
    28201 accgttgtcg ataatgttaa gacatatacg gatgtattgt cggaaggtgt cattaaagta
    28261 atggctaaga tgggaatttc gacagtgcaa agttatcaag gggcacaaat atttgaagcg
    28321 attggcttgt ctcatgatgt gattgatcgt tattttactg ggacacagtc taagttatct
    28381 ggtatttcga ttgatcaaat tgatgctgaa aataaagcac gtcaacaaag tgatgataat
    28441 tatcttgcat caggtagtac attccaatgg agacaacaag gtcaacatca tgcttttaat
    28501 ccggaatcta ttttcttatt gcagcacgca tgtaaagaaa atgactatgc gcaatttaaa
    28561 gcatactctg aagcggtgaa caaaaataga acagatcaca ttagacattt acttgaattt
    28621 aaagcatgta caccgattga catcgaccaa gttgaaccgg taagtgacat tgtcaaacgc
    28681 tttaatacag gggcgatgag ttatggatcg atttcagcgg aagcacatga aacgttagca
    28741 caagccatga accaattagg tggaaagagt aatagtggtg aaggtggcga agatgcaaaa
    28801 cgttatgaag tacaagttga tggaagcaac aaagtaagtg cgattaaaca agtttcttct
    28861 gggcggtttg gtgtaactag tgattattta caacatgcca aagaaattca aattaaagtt
    28921 gcgcaaggtg caaagcctgg tgaaggtggt caattacctg gtactaaggt atatccgtgg
    28981 attgcgaaga caagagggtc aacgccaggt atcggtctga tttcaccacc gccacatcat
    29041 gatatttatt caatagaaga tttagcgcaa ctgatacatg atttgaaaaa tgcgaataaa
    29101 gatgcagata tcgcggtaaa attagtttcg aaaacaggtg ttggtaccat tgcatctggg
    29161 gtggcaaaag catttgcaga taaaattgtc atcagtggtt acgatggtgg tacaggggct
    29221 tcacctaaaa cgagtattca gcatgccggt gttccttggg agattggttt agcagaaaca
    29281 catcaaacat taaaactaaa tgacttaaga agtcgtgtta agttagaaac agacggtaag
    29341 ttattaactg gtaaagatgt agcgtacgca tgtgcgcttg gagcggaaga atttggattt
    29401 gcaactgcac cattagtggt gttgggctgt ataatgatgc gtgtatgcca taaagataca
    29461 tgtccagtag gagttgcaac tcaaaacaaa gatttacgtg ctttatatag aggtaaagca
    29521 catcatgttg ttaattttat gcattttatt gcacaagaat taagagaaat tttagcatct
    29581 ttaggtttga aacgtgtaga agacttagtt ggaagaactg atttattaca acgatcatca
    29641 acattaaaag cgaatagcaa agcggctagt attgatgttg aaaaactgtt atgtcctttc
    29701 gatgggccaa acacaaaaga aattcaacaa aatcataatc ttgagcatgg atttgattta
    29761 acaaatttat atgaagtaac gaagccatat attactgaag ggcgtcgcta tacaggtagc
    29821 tttacagtaa ataatgaaca acgtgatgta ggagttatta caggtagtga gatttcgaaa
    29881 caatatggag aagcaggact tcctgaaaat acaattaatg tttatacgaa tggtcatgct
    29941 ggtcaaagtc ttgcagcata cgcaccgaaa ggcttaatga ttcatcatac tggagatgcg
    30001 aatgactatg ttggtaaagg attatctggt ggtacggtca ttgtcaaagc accttttgaa
    30061 gaacgacaaa atgaaattat tgctggtaac gtctcattct atggtgcgac aggtggtaag
    30121 gcatttatta acggtagtgc aggagaaaga ttctgtatta gaaatagtgg tgtagatgtt
    30181 gtcgttgaag gtatcggcga ccatggatta gagtacatga ctggtggaca tgtcattaat
    30241 ttaggtgatg taggtaagaa cttcggtcaa ggtatgagtg gtggtattgc ctacgttatc
    30301 ccgtctgatg tagaagcttt tgttgaaaat aatcaactag atacgctttc gtttacaaag
    30361 attaaacacc aagaagaaaa agcattcatt aagcaaatgc tggaagaaca tgtgtcacac
    30421 acgaatagta cgagagcgat tcatgtgtta aaacattttg atcgcattga agatgtcgtc
    30481 gttaaagtta ttcctaaaga ttatcaatta atgatgcaaa aaattcattt gcacaaatca
    30541 ttacatgaca atgaagatga agcgatgtta gctgcatttt acgatgacag taaaacaatc
    30601 gacgctaaac ataaaccagc cgttgtgtat taaggaaagg gggagatacg atgggtgaat
    30661 ttaaaggatt tatgaagtat gacaaacagt acttaggtga attatcactg gtagaccgtt
    30721 tgaagcatca taaagcatat caacaacgat ttactaaaga agatgcctct atccaaggtg
    30781 cgcgatgtat ggattgtgga acgccgtttt gtcaaaccgg acaacagtat ggtagggaaa
    30841 caataggttg tccaattgga aactacattc ctgaatggaa cgacttagtg tatcatcaag
    30901 attttaaaac tgcttatgaa cgcttaagcg aaacaaataa ctttcctgac tttacagggc
    30961 gtgtatgtcc tgcaccatgc gaaagtgctt gtgtgatgaa gattaataga gaatcgattg
    31021 cgattaaagg tattgaacgc acaattattg atgaagcttt tgaaaatggt tgggtagcgc
    31081 cgaaagttcc gagtcgccgt agagatgaaa aagtggcaat cgttggaagc ggtccagcag
    31141 gattaactgc tgctgaagaa cttaatctac taggatatca agtaactatt tatgaacgtg
    31201 ctagagaatc aggcggttta ttaatgtatg gtattccgaa tatgaaactt gataaagatg
    31261 tggttcgacg tcgtattaag ttaatggaag aagcgggcat tactttcatt aatggtgttg
    31321 aagtaggcgt tgatattgat aaagcaacgt tagaatctga gtatgatgcc attatattat
    31381 gtactggtgc acaaaaaggt agagatttac ctttagaagg acgcatgggt gatggtatac
    31441 atttcgctat ggattattta actgaacaaa cgcagctgtt aaatggagaa attgatgata
    31501 ttacaataac tgcaaaagat aagaatgtca ttatcattgg tgctggtgat acaggggcag
    31561 actgtgtagc gacagcatta agagaaaatt gtaaatcgat tgttcaattt aataaatata
    31621 cgaaattgcc agaagcaatt acatttacag aaaatgcatc atggccttta gcaatgccgg
    31681 tgtttaaaat ggactatgcg caccaagagt acgaagctaa gtttggtaag gaaccacgtg
    31741 catatggtgt tcaaacaatg cgttacgatg ttgacgataa aggacacata cgtggtttgt
    31801 atactcaaat tttagagcaa ggcgaaaatg gtatggtcat gaaagaagga cctgaaagat
    31861 tttggcctgc tgaccttgta ttattatcaa tcggcttcga aggtacagaa ccaactgtac
    31921 cgaatgcttt taacattaaa acggatagaa atcgaatcgt ggcggatgat acaaactatc
    31981 aaactaataa tgaaaaagta tttgctgctg gagatgctag acgtggtcaa agtttagttg
    32041 tatgggcgat taaagaaggt agaggcgtag cgaaagcagt agatcagtat ttagcgagta
    32101 aagtttgtgt ataatctttg tatggaaatg gtggttacgt tgacgttgtg gcatgctgaa
    32161 tcgagtttga aataatctag tatctatcaa cgtcacatgc tatcattgta acctaaaaac
    32221 aaaggtttgt aagacaacaa atagattaat tataagtagt gattttttac attcgtttat
    32281 aggtcaactg tagtggaaga caatgatttg tggtaatcat gtaatgctta aaaacaatat
    32341 tgacttttac agaacgttca tatatgataa atattgtgtt taggaggaat acccaagtcc
    32401 ggctgaaggg atcggtcttg aaaaccgaca ggggcttaac ggctcgcggg ggttcgaatc
    32461 cctcttcctc cgccatcaat atttatatta aatactatat ataatgaagg taagtgctca
    32521 aattttgagt atttaccttt tttatttgtc tttgaatgac tcgtaatttt tgataataga
    32581 aatgataagg cattgagatt ggaagggcat ttggcttgtg caatatacat agctaaatgt
    32641 cttttttgtt ttgtgaaata tgatggatgg cttgtgtgga caagtttgct atttatagat
    32701 atgcattttt caatttagga gttggccatg catctacact ttataatggt gagagcgtgg
    32761 tgaggtattg ttaataacgc aattgtagcg aggagttatt gctacatatg tcgttatggc
    32821 tcattgattt tctgaaatgg ctatcccaga taattgtgac aaaataaaaa tatttctttg
    32881 aaagccttta cataacttgt ctagacaagt tatactcatt ttaagacatt aagggagtga
    32941 aatatatggc tgtaaaaaga gaagatgtaa aagccatcgt aaccgctatt gggggaaaag
    33001 aaaatcttga agctgcaacg cattgtgtaa cacgattacg tttagtgctg aaggatgaaa
    33061 gtaaagttga taaagacgca ttaagtaata acgcgttggt caaggggcag tttaaagcag
    33121 accatcaata tcaaattgtc attggtccag gaacagtcga tgaagtgtat aagcagttta
    33181 ttgatgaaac aggtgctcaa gaagcttcga aagatgaagc gaaaaaagca gctgcacaaa
    33241 aagggaatcc agtacaacgt ttgatcaaat tgttggggga tatttttata ccaatattac
    33301 ctgcgattgt gacagctggt ttgttaatgg gaatcaataa tttacttaca atgaaaggtt
    33361 tatttggtcc aaaagcactt attgagatgt atccacaaat tgctgatatt tcaaacatca
    33421 ttaatgtgat tgcgagtacg gcatttattt tcttaccagc attaattggt tggagtagta
    33481 tgcgtgtatt tggtggtagt ccgattctag gcatagtctt aggtttgatt ttaatgcatc
    33541 cgcaattagt atctcagtat gatttggcaa aagggaatat tccgacgtgg aacttatttg
    33601 gcttagagat taagcagttg aattaccaag gtcaagtgtt gccagtttta attgcagctt
    33661 atgttctagc taaaattgaa aaaggattaa ataaagtcgt tcacgattcg ataaaaatgt
    33721 tggtcgttgg acccgtagcg cttttagtta ctggattttt agcatttatt atcattggac
    33781 cagttgcgtt attgattggt acaggtatta catctggtgt tacatttata ttccaacatg
    33841 caggatggct tggcggagca atatatggat tgttatatgc accacttgta attacaggac
    33901 tacaccatat gtttttagca gtagatttcc aattgatggg tagcagctta ggcggtacgt
    33961 atttatggcc aattgttgcg atttccaata tttgtcaggg ctctgcagca tttggagcat
    34021 ggtttgtcta taaacgtcgt aaaatggtta aagaagaagg cttggcatta acatcttgta
    34081 tttctggtat gttaggtgtt actgaaccag ctatgtttgg tgtgaactta cctctgaaat
    34141 atccatttat cgctgcgata tcaacgtctt gtgtattggg ggcaatcgtt ggtatgaata
    34201 acgtacttgg aaaagttggt gttggtggcg tgccagcatt catttcaatt caaaaagaat
    34261 tttggccagt atatcttatt gtgacagcta ttgctattgt tgtaccatgt atactaacaa
    34321 ttgtgatgtc tcattttagt aaacaaaaag cgaaagaaat tgttgaagat taataaaata
    34381 aaaaaagggg cgttcgttat ttggacgtcc tttattacgt tataaggtgg taattgtgtg
    34441 tcgaaagaaa tagattggag aaaatccgtt gtatatcaaa tttatcctaa gtcgtttaat
    34501 gatacgacgg ggaatggtat aggagatatc aacggaatta tagaaaaatt ggattatatc
    34561 aagttattgg gtgttgatta tatttggtta acaccagtgt atgaatcacc gatgaatgat
    34621 aatggctatg atatcagcaa ttatttagaa atcaacgaag actttggaac gatggatgat
    34681 tttgaaaagt taatcaaagt tgctcatcaa aaagacttga aagtgatgtt agatattgtc
    34741 attaatcata cgtcgacgga gcatgaatgg tttaaagaag cccgtaaatc taaagataac
    34801 ccttatagag attattactt tttcagatca tctgaagacg ggccgccaac aaattggcat
    34861 tctaaattcg gtggtaatgc atggaagtat gattctgaga cagatgaata ttatttacat
    34921 ttatttgatg tcagtcaagc tgatttaaat tgggataatc cggaagtacg tcaatcgtta
    34981 tatcgcatag tcaatcattg gatagacttc ggcgttgatg gttttcgatt tgatgtcatt
    35041 aacttaattt ctaaaggtga atttaaggac tctgacaaaa taggtaaaga attttatacg
    35101 gatggtccta gagtgcatga gtttctgcat gaattaaatc gtcaaacgtt tggtaacact
    35161 gacatgatga ctgtaggaga aatgtcttcg acgacgattg aaaattgtat taagtataca
    35221 cagccagaac gccaagaatt gaatagtgtt tttaattttc atcatctaaa ggttgattat
    35281 gttgatggtg aaaagtggac aaatgcgaag cttgattttc ataagttaaa ggaaattctg
    35341 atgcaatggc aacgaggtat ttatgacggt ggcggatgga acgcgatttt ctggtgtaat
    35401 catgatcagc cacgggtagt gtctagattt ggtgatgata cgtcggaaga gatgaggata
    35461 caaagtgcta aaatgttagc tatcgcactg catatgttgc aagggacgct atatatttac
    35521 caaggggaag aaattggtat gacggaccca cattttacat caatagcaca atatcgcgat
    35581 gttgaatcga ttaatgccta ccatcagttg ttaagtgaag ggcatgctga agcggatgta
    35641 ttagcgattt taggacagaa gtcacgagac aattcgagaa cgcctatgca atggagtgat
    35701 gatgttaatg ctggatttac agctggtaag ccttggattg atatttcgga aaattatcat
    35761 caggtcaacg ttagacaagc acttcagaat aaagattcta ttttctacac gtatcaaaaa
    35821 ttaatacaat taagacatac gcatgatatt attacgtatg gagacattgt gccacgtttt
    35881 atggatcatg atcatttatt tgtttatgaa cgtcattata agaatcaaca atggctagta
    35941 attgcgaatt tctcagcatc ggctgttgat ttgccagaag gattggctag agaaggtcgt
    36001 gttgtgattc aaacaggcac agtggaaaat aatacgataa gcgggtttgg tgcaattgta
    36061 atcgaaacaa acgcgtaaaa taaattgagt ggatgcgttt atatggcgaa acaaaaaaag
    36121 tttatgaaga tttatgaggc gttgaaagaa gatatattaa acgggcagat tcaatatggt
    36181 gaacaaattc cgtctgaaca tgatttggtg caattgtacc agtcatctcg agagaccgtg
    36241 cgtaaggcat tagatttgtt ggcattagac ggcatgattc aaaagattca tggtaaaggg
    36301 tcacttgtca tttatcagga ggttacagag tttccatttt ctgaacttgt tagttttaaa
    36361 gaaatgcaag aagaaatggg cgtcgcatat ttaactgaag ttgttgtgaa tgaggttgtt
    36421 gaagcgcatg aagttccaga agttcaacat gctttaaaca tcaattctag tgaatcactc
    36481 attcatattg ttagaactcg tcggcttaac caacatgtga agattgttga tgaagattat
    36541 tttctaaagt cgattgtttc agatataggt aatgatgttg cgagtgattc tatttatgat
    36601 tatttggaaa aggtattaaa tcttaatatt agttattcaa gtaagtctat tacttttgaa
    36661 ccgtttgatg agcaagcata tcaattgttt ggtgatgtat cggtggctta ttcagcaaca
    36721 gttcgaagta ttgtgtattt agaaaataca atgccgtttc aatataatat ttcaaaacat
    36781 cttgcaaatg aatttaaatt taatgacttc tcaagacgtc gtacaaagta aacaatgata
    36841 taaatgattt atacttgcaa ttaactatta aaatatagta atatatatct tgccgtgcta
    36901 ggtggggagg tagcggttcc ctgtactcga aatccgcttt atgcgaggct taattccttt
    36961 gttgaggccg tatttttgcg aagtctgccc aaagcacgta gtgtttgaag atttcggtcc
    37021 tatgcaatat gaacccatga accatgtcag gtcctgacgg aagcagcatt aagtggatca
    37081 tcatatgtgc cgtagggtag ccgagattta gctaacgact ttggttacgt tcgtgaatta
    37141 cgttcgatgc ttaggtgcac ggttttttat tttttaaata ttaaaccgat tattaagagt
    37201 tgaaaatata tatttattta tagaagctac tttcttgaag acaattcagc gtattatacg
    37261 tggaacatgt ttgtgggaag tagctttttt atatgtgaag tttgattcaa gtgaactcga
    37321 tgtacagttt gaatgatttt tgtgtcaatg aaaagtaaga agttataatt tgatgataaa
    37381 gaaatgatgg tgaaatgagg gggagtatct tacaatagaa ttattaatga gatacgttat
    37441 gattattgac aatcaaatgc ctacggagga catatgcaaa tatatttaag tactttaaca
    37501 gagttagatt atgataaatc tttaaatagt attgaagaaa gttttgatga taatcctgaa
    37561 acgagttggc aagcacgtgc gaaagtaaaa catttaagaa aatctccttg ctataatttt
    37621 gaattagaag taatagcgaa aaatgaaaat aacgatgtcg ttggacacgt tttattaatt
    37681 gaagtagaaa ttaatagtga tgataagacg tattatggtt tggcgattgc ctctttatca
    37741 gttcatcctg aattacgtgg acaaaaatta ggtcgtggct tggttcaagc agtagaagag
    37801 cgtgccaaag cacaagagta tagtacggtt gttgtagacc attgttttga ctactttgaa
    37861 aagttgggtt atcaaaatgc tgctgagcat gacattaaat tagaatctgg tgatgcaccg
    37921 ttacttgtaa aatatttatg ggataatttg acggatgcac cacacggaat cgtaaaattt
    37981 ccagaacatt tttattaatt gttcaattaa gaagtaaagg tattatcatg ctataatgag
    38041 aggtaattgt ttatggaggt gctaacttga attatcaagc cttatatcgt atgtacagac
    38101 cccaaagttt cgaggatgtc gtcggacaag aacatgtcac gaagacattg cgcaatgcga
    38161 tttcgaaaga aaaacagtcg catgcttata tttttagtgg tccgagaggt acggggaaaa
    38221 cgagtattgc caaagtgttt gctaaagcaa tcaactgttt aaatagcact gatggagaac
    38281 cttgtaatga atgtcatatt tgtaaaggca ttacgcaggg gactaattca gatgtgatag
    38341 aaattgatgc tgctagtaat aatggcgttg atgaaataag aaatattaga gacaaagtta
    38401 aatatgcacc aagtgaatcg aaatataaag tttatattat agatgaggtg cacatgctaa
    38461 caacaggtgc ttttaatgcc cttttaaaga cgttagaaga acctccagca cacgctattt
    38521 ttatattggc aacgacagaa ccacataaaa tccctccaac aatcatttct agggcgcaac
    38581 gttttgattt taaagcaatt agcctagatc aaattgttga acgtttaaaa tttgtagcag
    38641 atgcacaaca aattgaatgt gaagatgcag ccttggcatt tatcgctaaa gcgtctgaag
    38701 ggggtatgcg tgatgcatta agtattatgg accaagctat tgcatttggt gatggtacgt
    38761 taacattgca agatgcgttg aatgtcacag gtagcgtaca tgatgaagcg ttggatcact
    38821 tgtttgatga tattgtacaa ggtgacgtac aagcatcttt taaaaaatac catcagttta
    38881 taacagaagg taaagaagtg aatcgcctaa taaatgatat gatttatttt gtcagagata
    38941 cgattatgaa taaaacatct gagaaagata ctgagtatcg agcactgatg aacttagaat
    39001 tagatatgtt atatcaaatg attgatctta ttaatgatac attagtgtcg attcgtttta
    39061 gtgtgaatca aaacgttcat tttgaagtgt tgttagtaaa attagctgag cagattaagg
    39121 gtcaaccaca agtgattgcg aatgtagctg aaccagcaca aattgcttca tcgccaaaca
    39181 cagatgtatt gttgcaacgt atggaacagt tagagcaaga actaaaaaca ctaaaagcac
    39241 aaggagtgag tgtcgctcct actcaaaaat cttcgaaaaa gcctgtgaga ggtatacaaa
    39301 aatctaaaaa tgcattttca atgcaacaaa ttgcaaaagt gctagataaa gcgaataagg
    39361 cagatatcaa attgttgaaa gatcattggc aagaagtgat tgatcatgcc aaaaacaatg
    39421 ataaaaaatc actcgttagt ttattgcaaa attcggaacc tgtggcggca agtgaagatc
    39481 acgtacttgt gaaatttgag gaagagatcc attgtgaaat cgtcaataaa gacgacgaga
    39541 aacgtagtag catagaaagt gttgtatgta atatcgttaa taaaaacgtt aaagttgttg
    39601 gtgtaccatc agatcaatgg caaagagttc gaacggagta tttacaaaat cgtaaaaacg
    39661 aaggcgatga tatgccaaag caacaagcgc aacaaacaga tattgctcaa aaagcaaaag
    39721 atcttttcgg tgaagaaact gtacatgtga tagatgaaga gtgatacatg acaagcgata
    39781 taatcgtatg tataatgaaa gaaacatcat tttattgata aatatttatt gattttcaag
    39841 gaggaaatgg aatatgcgcg gtggcggaaa catgcaacaa atgatgaaac aaatgcaaaa
    39901 aatgcaaaag aaaatggctc aagaacaaga aaaacttaaa gaagagcgta ttgtaggaac
    39961 agctggcggt ggcatggttg cagttactgt aactggtcat aaagaagttg tcgacgttga
    40021 aatcaaagaa gaagctgtag acccagatga tattgaaatg ctacaagact tagtgttagc
    40081 agctactaat gaagcgatga ataaagctga tgagcttact caagaacgtt taggtaaaca
    40141 tactcaaggc ttaaacatcc ctggaatgtg atcatagatg cattatccag aacctatatc
    40201 aaaacttatt gatagcttta tgaaattgcc aggcattggt ccaaagacag cccaacgtct
    40261 ggcttttcat accttagata tgaaagaaga cgatgttgtt cagtttgcca aagcattagt
    40321 agatgttaaa agagaattaa catattgtag cgtatgtggt cacattactg aaaatgatcc
    40381 atgttatatt tgtgaagata agcaaagaga tcgttcagtt atttgtgttg tggaagatga
    40441 caaagatgtc atagctatgg aaaaaatgag agaatacaaa ggtttatatc acgttttaca
    40501 tgggtctatt tcgcctatgg atggcattgg accagaagat attaatattc cttcattgat
    40561 tgaacgctta aaaagcgatg aagttagcga attaatctta gctatgaacc cgaacttaga
    40621 gggggaatct acagccatgt atatttctag attagttaag cctataggta tcaaagtgac
    40681 gagattagca caagggttat cggtaggtgg cgatttagag tatgctgacg aagtaacttt
    40741 atctaaagca atcgcaggta gaacagaaat gtaatgtctt ctattaaaca tttttgaatt
    40801 taatactata gtaagaaaag tcacagtgta atcattgtgg ctttttttat ggtgtggtgt
    40861 gatgtactac tttatttgcg gtgtggcggt ggtatggttt acctagtttt actgagggat
    40921 gggtaatctt taggaagcaa gccgttggtt gtgatttgtt acttctaata gtaatgatgt
    40981 gaattggatt atcgaattag atctacggtt atggtgtgtt ggtgctatta atttgataaa
    41041 tgcggttgat ggttaatgac tatgcaaatg aaattctttt gtaattgaaa tgatagatgc
    41101 tggcttagta gttgtacttc tttggtctaa agtttattaa atcagcctgt atagcggtgt
    41161 tttgagaggt tatttaaaac ttgtaaattt atttttaatt tctggtaaaa aaataaagtt
    41221 ctgttttgcg gtttttttga ttgatatggt tagagaaaaa tctgtttctt gttctaaaaa
    41281 acgtactatt tataagtggg gattttttaa gttcgatttt taggataagg gcgttcagta
    41341 cagatggcaa aggtgtaatt tttactgttg ttaagcagtt tgaaagcctg tatagtattt
    41401 atttgttgag gcaaacaaaa caactcaact taagaaataa cttgaattac taacgaaaat
    41461 taattttaaa aagtt
//
LOCUS       DAHHOJ010000010        40319 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.010, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000010 DAHHOJ010000000
VERSION     DAHHOJ010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 40319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40319
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.010"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            180..578
                     /gene="nrdI"
                     /locus_tag="NBH32_000433"
     CDS             180..578
                     /gene="nrdI"
                     /locus_tag="NBH32_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCD1514065.1"
                     /translation="MKIIYFSFTGNVRRFIKRTELENTLEITAENCMEPVHEPFIIVT
                     GTIGFGEVPEPVQSFLEVNHQYIRGVAASGNRNWGLNFAKAGRTISEEYNVPLLMKFE
                     LHGKNKDVIEFKNKVGNFNENHGREKVQSY"
     gene            541..2646
                     /gene="nrdE"
                     /locus_tag="NBH32_000434"
     CDS             541..2646
                     /gene="nrdE"
                     /locus_tag="NBH32_000434"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000855513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCD1514066.1"
                     /translation="MKTMEEKKYNHIELNNEVTKRREDGFFSLEKDQEALVAYLEEVK
                     DKTIFFDTEIERLRYLVDNDFYFNVFDIYSEADLIEITDYAKSIPFNFASYMSASKFF
                     KDYALKTNDKSQYLEDYNQHVAIVALYLANGNKAQAKQFISAMVEQRYQPATPTFLNA
                     GRVRRGELVSCFLLEVDDSLNSINFIDSTAKQLSKIGGGVAINLSKLRARGEAIKGIK
                     GVAKGVLPIAKSLEGGFSYADQLGQRPGAGAVYLNIFHYDVEEFLDTKKVNADEDLRL
                     STISTGLIVPSKFFDLAKEGKDFYMFAPHTVKEEYGVTLDDIDLEKYYDDMVANPNVE
                     KKKKNAREMLNLIAQTQLQSGYPYLMFKDNANRVHPNSNIGQIKMSNLCTEIFQLQET
                     SIINDYGIEDEIKRDISCNLGSLNIVNVMESGKFRDSVHSGMDALTVVSDVANIQNAP
                     GVRKANSELHSVGLGVMNLHGYLAKNKIGYESEEAKDFANIFFMMMNFYSIERSMEIA
                     KERGIKYQDFEKSDYANGKYFEFYTTQEFEPQFEKVRELFDGMVIPTSEDWKKLQQDV
                     EQYGLYHAYRLAIAPTQSISYVQNATSSVMPIVDQIERRTYGNAETFYPMPFLSPQTM
                     WYYKSAFNTDQMKLIDLIATIQTHIDQGISTILYVNSEISTRELARLYVYAHYKGLKS
                     LYYTRNKLLSVEECTSCSI"
     gene            2766..3737
                     /gene="nrdF"
                     /locus_tag="NBH32_000435"
     CDS             2766..3737
                     /gene="nrdF"
                     /locus_tag="NBH32_000435"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCD1514067.1"
                     /translation="MIAVNWNTQEDMTNMFWRQNISQMWVETEFKVSKDIASWKTLSE
                     AEQDTFKKALAGLTGLDTHQADDGMPLVMLHTTDLRKKAVYSFMAMMEQIHAKSYSHI
                     FTTLLPSSETNYLLDEWVLEEPHLKYKSDKIVANYHKLWGKEASIYDQYMARVTSVFL
                     ETFLFFSGFYYPLYLAGQGKMTTSGEIIRKILLDESIHGVFTGLDAQHLRNELSESEK
                     QKADQEMYKLLNDLYLNEESYTKMLYDDLGITEDVLNYVKYNGNKALSNLGFEPYFEE
                     REFNPIIENALDTTTKNHDFFSVKGDGYVLALNVEALQDDDFVFDNK"
     gene            4107..5078
                     /locus_tag="NBH32_000436"
     CDS             4107..5078
                     /locus_tag="NBH32_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1514068.1"
                     /translation="MKYLLKGKILLLLLILLTIISLFIGVSELSIKDIFHLSDSQQNI
                     LFSSRIPRTMSILIAGSSLALAGLIMQQMMQNKFVSPTTAGTMEWAKLGILIALLFFP
                     TGHILLKLVFAVICSIGGTFLFVKIIDFIKVKDVIFVPLLGIMMGGIVASFTTFISLR
                     TNAVQSIGNWLNGNFAIITSGRYEILYLSIPLLALTYLFANHFTIVGMGKDFTNNLGL
                     SYEKLINIALFITATITALVVVTVGTLPFLGLVIPNIISIYRGDHLKNAIPHTMMLGA
                     IFVLFSDIVGRIVVYPYEINIGLTIGVFGTIIFLILLMKGRKNYAQQ"
     gene            5065..6021
                     /locus_tag="NBH32_000437"
     CDS             5065..6021
                     /locus_tag="NBH32_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1514069.1"
                     /translation="MRNNNKKIMLLIVVTFLISMLYLFVGIDFEIFEYQFSNRLRKFI
                     LIILVGAAIATSVVIFQAITNNRLLTPSIMGLDAVYLFIKVLPVFLFGIQSVWVTNVY
                     LNFILTLITMVLFALILFQGIFKIGHFSIYFILLIGVLLGTFFRSITGFIQLIMDPES
                     FLAIQSSMFANFNASNSNLVSFSAVLLVILLVITIFLLPYLDVLLLGRAEAINLGISY
                     EKLTRILLVIVSVLVSVSTALVGPITFLGLLTVNLAHELMKTYEHKYILIATICLSWI
                     SLFSAQWVVENVFEATTEMSILIDLIGGSYFIYLLVRRRNAQ"
     gene            6018..6779
                     /locus_tag="NBH32_000438"
     CDS             6018..6779
                     /locus_tag="NBH32_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514070.1"
                     /translation="MIQVGNLTKTINNQMILDDISIEIEKGKLTSLIGPNGAGKSTLL
                     SAICRLIRFDEGEVKIDGQLMSDYKNNDLSKKISILKQTNHTEMNITVEQLVNFGRFP
                     YSKGRLTKEDHEIVNDALDLLQLQDIRKRNIKSLSGGQRQRAYIAMTIAQDTEYILLD
                     EPLNNLDMKHAVQIMQTLKMLAHKMNKAIVIVLHDINFASCYSDQIVALKNGQLVKSD
                     LKDNVIQSSVLSDLYDMDIQIEHIKNQRICLYFKD"
     gene            6898..7926
                     /locus_tag="NBH32_000439"
     CDS             6898..7926
                     /locus_tag="NBH32_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1514071.1"
                     /translation="MKKTVLYLVVAVMFLLAACGNNSDKEQSKSETKGSKDTVKIENN
                     YKMRGEKKDGSDAKKVKETVEVPKNPKNAVVLDYGALDVMKEMGLSDKVKALPKGEGG
                     KSLPNFLESFKDDKYTNVGNLKEVNFDKIAATKPEVIFISGRTANQKNLDEFKKAAPK
                     AKIVYVGADEKNLIGSMKQNTENIGKIYDKEDKAKELNKDLDNKIASMKDKTKKFNKS
                     VMYLLVNEGELSTYGPKGRFGGLVYDTLGFNAVDKKVSNSNHGQNVSNEYINKENPDV
                     ILAMDRGQAVSGKSTAKQALNNPVLKNVKAIKEDKVYNLDPKLWYFAAGSTTTTIKQI
                     EELEKVVK"
     gene            complement(8243..8557)
                     /locus_tag="NBH32_000440"
     CDS             complement(8243..8557)
                     /locus_tag="NBH32_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514072.1"
                     /translation="MPIVYGSLIDTETRCRHYFTEEDIIAIKFKCCNKYYPCYKCHNE
                     FEKHAIKRWSEPSFNEKAILCGVCKHELTINEYMMVERCPNCQSRFNNRCKYHYHIYF
                     EI"
     gene            complement(8575..9498)
                     /locus_tag="NBH32_000441"
     CDS             complement(8575..9498)
                     /locus_tag="NBH32_000441"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCD1514073.1"
                     /translation="MINKDIYQALQQLIPNEKIKVDEPLKRYTYTKTGGNADFYITPT
                     KNEEVQAVVKYAYQNEIPVTYLGNGSNIIIREGGIRGIVISLLSLDHIEVSDDAIIAG
                     SGAAIIDVSRVARDYALTGLEFACGIPGSIGGAVYMNAGAYGGEVKDCIDYALCVNEQ
                     GSLIKLTTKELELDYRNSIIQKEHLVVLEAAFTLAPGKMTEIQAKMDDLTERRESKQP
                     LEYPSCGSVFQRPPGHFAGKLIQDSNLQGHRIGGVEVSTKHAGFMVNVDNGTATDYEN
                     LIHYVQKTVKEKFGIELNREVRIIGEHPKES"
     gene            complement(9625..10143)
                     /locus_tag="NBH32_000442"
     CDS             complement(9625..10143)
                     /locus_tag="NBH32_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HCD1514074.1"
                     /translation="MYSNVQPFINSDLTNCFQSQYTEIESLLFNLLDSPVKYTKHIGG
                     THHFKYSTEPILDILVGVENLHDITSLDEKRLNYVGFYRLHHKYNKKVMMAKFNNFHE
                     LKQQVRLHIIQMNTQTFESYLALDYLLSTNDEIALQFNTKKHQILQHSFTIRQYEMQK
                     QKYFEKLCKKYL"
     gene            10263..11141
                     /locus_tag="NBH32_000443"
     CDS             10263..11141
                     /locus_tag="NBH32_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EMYY motif lipoprotein"
                     /protein_id="HCD1514075.1"
                     /translation="MKKIVIIAVLAILFVVISACGNKEKEAQHQFTKQFKDVEQKQKE
                     LQHVMDNIHLKEIDHLSKTDTTDKNSKEFKALQEDVKNHLIPKFEAYYKSAKNLPDDT
                     MKVKKLKKEYMTLANEKKDAIYQLKKFIGLCNQSIKYNEDILDYTKQFEKNRYKVESE
                     IKLADNKSEATNLTTKLEHNNKALRDTAKKNLDDSKENEVKGAIKNHIMPMIEKQITD
                     INQTNISDKHVNNARKNAIEMYYSLQNYYNTRIETIKVSEKLSKVDVDKLPKKGIDIT
                     HGDKAFEKKLEKLEEK"
     gene            11295..11615
                     /gene="ytxJ"
                     /locus_tag="NBH32_000444"
     CDS             11295..11615
                     /gene="ytxJ"
                     /locus_tag="NBH32_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol system redox-active protein YtxJ"
                     /protein_id="HCD1514076.1"
                     /translation="MAIKLSSIDQFEQVIEENKYVFVLKHSETCPISANAYDQFNKFL
                     YERDMDGYYLIVQQERDLSDYIAKKTNVKHESPQAFYFVNGEMVWNRDHGDINVSSLA
                     QAEE"
     gene            12040..13164
                     /locus_tag="NBH32_000445"
     CDS             12040..13164
                     /locus_tag="NBH32_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HCD1514077.1"
                     /translation="MKVLVAMDEFHGIISSYQANRYVEEAVASQIETADVVQVPLFNG
                     RHELLDSVFLWQSGQKYRIPVHDADMNEVEGVYGQTDTGMTVIEGNLFLKGKKPIVER
                     TSYGLGEMIKHALDNDAKHVVISLGGIDSFDAGAGMLQALGAQFYDDEGRVVDMRQGA
                     GVIKYIRRMDMSNLHPKMETARIQVMSDFSSRLYGKQSEIMQTYDAHQLNHNQAAEID
                     NLIWYFSELFKSELKIAIGPVERGGAGGGIAAVLNGLYQAEILTSHALVDQLTHLENL
                     VEQADLIIFGEGLNENDQLLETTTLRIAELCHKHQKVAIAICATAEKFDLFESQGVTA
                     MFNTFIDMPESYTDFKMGLQIRHYTVQSLKLLKTHFNVEV"
     gene            complement(13352..14578)
                     /gene="pepT"
                     /locus_tag="NBH32_000446"
     CDS             complement(13352..14578)
                     /gene="pepT"
                     /locus_tag="NBH32_000446"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCD1514078.1"
                     /translation="MKNQLIDRLTRYTTIDTQSDPKSTTTPSTEKQWDLLHLLEKELQ
                     QLGLPTDLDENGYLFATLESNIDVDVPTVGFLAHVDTSPDFNASNVKLQIIENYDGKP
                     YKLGNTKRVLDPKVFPELNSLVGHTLMVTDGTSLLGADDKAGIVEIMEAICYLQEHPE
                     IKHGTIRIGFTPDEEIGRGPHKFDVDRFNADFAYTMDGSQYGELQYESFNAAEAIITC
                     HGVNVHPGSAKNAMVNAIRLGEQFDSLLPDSEVPERTEGYEGFYHLMNFEGTVEKATL
                     QYIIRDHDKKQFELRKKRILEIRDDINAHFENYPVKVDISDQYFNMAEKILPLPHIID
                     IPKRVFAKLDIPANTEPIRGGTDGSQLSFMGLPTPNIFTGCGNFHGPYEYASIDVMEK
                     AVQVIIGIVEDIAENH"
     gene            complement(14592..15086)
                     /locus_tag="NBH32_000447"
     CDS             complement(14592..15086)
                     /locus_tag="NBH32_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1514079.1"
                     /translation="MLFYLFHFTISFISTVLFSIIFNAPKRLLVACGFVGAIAWTIYQ
                     LTVDLEFGKVGASFLGSLILGLMSHTMSRRYKRPVIIFIVPGIIPLVPGGAAYQATRF
                     LVSNDYTSAVNTFLEVTLISGAIAFGILVSEILYYLYTRIKQLYGKIKGKTYKKSYNM
                     NNRV"
     gene            complement(15104..15865)
                     /locus_tag="NBH32_000448"
     CDS             complement(15104..15865)
                     /locus_tag="NBH32_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD1514080.1"
                     /translation="MPDSITIIDENKVIDVVLIAGRILLESGAETYRVEDTMNRIAHS
                     YGLHNTYSFVSSTAIIFSLNDRTSTRLIRVQERTTDLEKIALTNSLSRKISNKELTID
                     EAKSEFIHLQHASLQYSFLTNFFAAAIACGFFLFMFGGVASDCWIAVIAGGSAFLTFS
                     FVQRYIQIKFFSEFVAAAVVISIAATFTKLGIATNQDIITIASVMPLVPGILITNAIR
                     DLLAGELLAGMSRGVEAALTAFAIGAGVAIVLLII"
     gene            complement(16061..17131)
                     /locus_tag="NBH32_000449"
     CDS             complement(16061..17131)
                     /locus_tag="NBH32_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCD1514081.1"
                     /translation="MFEAFIYNISVIVAGIYLFHRLQYSENKRMVFSKAYVTVLMTIV
                     SLLLSVYPIPYREDYLIHLTFVPLLFLGRFTNMVYTLSATVIVAIVEIVVFNNSIMYG
                     VTLIVIAAVTSAIGPFLKQNDVLSLLILNVVTIIILFGVALVSPIYTLSEVIILIPIS
                     LIITLASAITFVDIWHFFSLVNRYENEDKYDYLTGLGNVKEFDRHLNEISRKAEKEHQ
                     SIALLLIDIDGFKDVNDTYSHKSGDAVLKQMSQLLKNYVPNQFKIFRNGGEEFSVVIH
                     NYSLDQSVKLAENIRSGVEKSSFHLPNKEVIKLSVSIGVGYLTDDDPKSQRKVFKDAD
                     DMVHVAKNQGRNKVMFNPIINL"
     gene            17449..18504
                     /locus_tag="NBH32_000450"
     CDS             17449..18504
                     /locus_tag="NBH32_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HCD1514082.1"
                     /translation="MVTLLLVAVTMIVSLTITPIVIAISKRLNLVDKPNFRKVHTKPI
                     SVMGGTVILFSFLIGIWIGHPIETEIKPLIIGAIIMYVLGLVDDIYDLKPYIKLAGQI
                     AAALVVAFYGVTIDFISLPMGTTIHFGFLSIPITVIWIVAITNAINLIDGLDGLASGV
                     SAIGLITIGFIAILQANIFITMICCVLLGSLIGFLFYNFHPAKIFLGDSGALMIGFII
                     GFLSLLGFKNITIIALFFPIVILAVPFIDTLFAMIRRVKKGQHIMQADKSHLHHKLLA
                     LGYTHRQTVLLIYSISILFSLSSIILYVSPPLGVVLMFILIIFSIELIVEFTGLIDNN
                     YRPILNLISRKSSHKED"
     gene            complement(18668..19309)
                     /locus_tag="NBH32_000451"
     CDS             complement(18668..19309)
                     /locus_tag="NBH32_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HCD1514083.1"
                     /translation="MTQNIITVKKEHIIENVISKSKFIAHIKPVQNEDEAKAFIAAIK
                     KEHKDATHNCSAYTIGPEMNIQKANDDGEPTGTAGVPMLDILKKLDVHNACVVVTRYF
                     GGIKLGGGGLIRAYSGAVRDVIYDVGRVELREAVPCIVTLNYDQTGKFEYELASTHFM
                     LRNQTYTDKVSYYIDVVKSDYEAFINFLNQMTAGNFDLTEETPKQLPFDIPTE"
     gene            19453..20319
                     /gene="fakB1"
                     /locus_tag="NBH32_000452"
     CDS             19453..20319
                     /gene="fakB1"
                     /locus_tag="NBH32_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000686350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB1"
                     /protein_id="HCD1514084.1"
                     /translation="MKIAVMTDSTSYLSQDLIDKYNIQIAPLSVTFDDGKNFTESNEI
                     AIEEFYNKMASSQTIPTTSQPAIGEWITKYEMLRDQGYTDIIVICLSSGISGSYQSSY
                     QAGEMVEGVNVHAFDSKLAAMIEGCYVLRAIEMVEEGYEPQQIIDDLTNMREHTGAYL
                     IVDDLKNLQKSGRITGAQAWVGTLLKMKPVLKFEDGKIIPEEKVRTKKRAIQTLEKKV
                     LDIVKDFEEVTLFVINGDHFEDGQALYKKLQEDCPSGYQVAYSEFGPVVAAHLGSGGL
                     GLGYVGRKIRLT"
     gene            20683..21765
                     /locus_tag="NBH32_000453"
     CDS             20683..21765
                     /locus_tag="NBH32_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011382223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1514085.1"
                     /translation="MDNVTRYKITESSQSSSQAYYHLSFELSEQQSYASEHIVRAIRM
                     RQTILLYAVTGAGKTEMMFQGIQYARRQGDNIAIVSPRVDVVVEISKRIKDAFLNEDI
                     DILHQQSSQQFEGHFVVCTVHQLYRFKQHFDTIFIDEVDAFPLSMDKSLQQALKSSSK
                     VEHATIYMTATPPKQLLSEIPHENVIKLPARFHKKSLPVPKYRYFKLNNNKIQKMLYR
                     ILQDQINKQRYTLVFFNNIETMIKTFSVYKQKITKLTYVHSEDVFRFEKVEQLRNGQF
                     DVIFTTTILERGFTMANLDVVVIDAHQYTQEALIQIAGRVGRKLECPTGKVLFFHEGV
                     SMNMIQAKKEIQKMNKLALKRGWIDE"
     gene            21758..22432
                     /locus_tag="NBH32_000454"
     CDS             21758..22432
                     /locus_tag="NBH32_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HCD1514086.1"
                     /translation="MNNCLSCGAKLYENITIYNLFKKPNRLCDRCKENWDNIKLDIKA
                     RRCSRCLKYLNQNEAYCLDCKFLSAHFNLMEQLYCQFQYDGLMKEMIHQYKFLKDYYL
                     CELLAHLIEIPQTSYDYIVPIPSSPAHDLSRTFNPVEAVLKAKGIRFDKILKMSNRPK
                     QSHLTKKERLADENPFIIDTELDLNGKEILLVDDIYTTGLTIHRAGCKLYAKNIRKFK
                     VFAFAR"
     gene            22493..23065
                     /gene="raiA"
                     /locus_tag="NBH32_000455"
     CDS             22493..23065
                     /gene="raiA"
                     /locus_tag="NBH32_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCD1514087.1"
                     /translation="MIRFEIHGDNLTITDAIRNYIEEKIGKLERYFNDVPNAVAHVKV
                     KTYSNSATKIEVTIPLKNVTLRAEERNDDLYAGIDLINNKLERQVRKYKTRINRKSRD
                     RGDQEVFVAELQEMQETQVDNDAYDDNEIEIIRSKEFSLKPMDSEEAVLQMNLLGHDF
                     FVFTDRETDGTSIVYRRKDGKYGLIQTSEQ"
     gene            23480..26011
                     /gene="secA"
                     /locus_tag="NBH32_000456"
     CDS             23480..26011
                     /gene="secA"
                     /locus_tag="NBH32_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCD1514088.1"
                     /translation="MGFLSKILDGNNKEIKQLGKLADKVIALEEKTAILTDEEIRNKT
                     KQFQTELADIDNVKKQNDYLDKILPEAYALVREGSKRVFNMTPYKVQIMGGIAIHKGD
                     IAEMRTGEGKTLTATMPTYLNALAGRGVHVITVNEYLSSVQSEEMAELYNFLGLTVGL
                     NLNSKTTEEKREAYAQDITYSTNNELGFDYLRDNMVNYSEDRVMRPLHFAIIDEVDSI
                     LIDEARTPLIISGEAEKSTSLYTQANVFAKMLKQDEDYKYDEKTKAVHLTEQGADKAE
                     RMFKVENLYDVQNVDVISHINTALRAHVTLQRDVDYMVVDGEVLIVDQFTGRTMPGRR
                     FSEGLHQAIEAKEGVQIQNESKTMASITFQNYFRMYNKLAGMTGTAKTEEEEFRNIYN
                     MTVTQIPTNKPVQRNDKSDLIYISQKGKFDAVVEDVVEKHKAGQPVLLGTVAVETSEY
                     ISNLLKKRGIRHDVLNAKNHEREAEIVAGAGQKGAVTIATNMAGRGTDIKLGEGVEEL
                     GGLAVIGTERHESRRIDDQLRGRSGRQGDKGDSRFYLSLQDELMIRFGSERLQKMMSR
                     LGLDDSTPIESKMVSRAVESAQKRVEGNNFDARKRILEYDEVLRKQREIIYNERNSII
                     DEEDSSQVVDAMLRSTLQRSINYYINTADDEPEYQPFIDYINDIFLQEGDITEDDIKG
                     KDAEDIFEVVWAKIEAAYQSQKDILEEQMNEFERMILLRSIDSHWTDHIDTMDQLRQG
                     IHLRSYAQQNPLRDYQNEGHELFDIMMQNIEEDTCKFILKSVVQVEDNIEREKTTEFG
                     EAKHVSAEDGKEKVKPKPIVKGDQVGRNDDCPCGSGKKFKNCHGK"
     gene            26053..26151
                     /locus_tag="NBH32_000457"
     CDS             26053..26151
                     /locus_tag="NBH32_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514089.1"
                     /translation="MLWEESFYFTSVKYFKKCEEVVKRCYVLSFKK"
     gene            26326..27436
                     /gene="prfB"
                     /locus_tag="NBH32_000458"
     CDS             join(26326..26397,26399..27436)
                     /gene="prfB"
                     /locus_tag="NBH32_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667688.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCD1514090.1"
                     /translation="MELSEIKRNIDKYNQDLTQIRGSLDLENKETNIQEYEEMMAEPN
                     FWDNQTKAQDIIDKNNALKAIVNGYKTLQAEVDDMDATWDLLQEEFDEEMKEDLEQEV
                     IYFKAKVDEYELQLLLDGPHDANNAILELHPGAGGTESQDWANMLFRMYQRYCEKKGF
                     KVETVDYLPGDEAGIKSVTLLIKGHNAYGYLKAEKGVHRLVRISPFDSSGRRHTSFAS
                     CDVIPDFNNDEIEIEINPDDITVDTFRASGAGGQHINKTESAIRITHHPSGIVVNNQN
                     ERSQIKNREAAMKMLKSKLYQLKLEEQAREMAEIRGEQKEIGWGSQIRSYVFHPYSMV
                     KDHRTNEETGKVDAVMDGDIGPFIESYLRQTMSHD"
     gene            27589..27696
                     /locus_tag="NBH32_000459"
     CDS             27589..27696
                     /locus_tag="NBH32_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514091.1"
                     /translation="MCLLKQHMQCAFSNITYGDFRGIELIVENVLNYMV"
     gene            27844..28683
                     /locus_tag="NBH32_000460"
     CDS             27844..28683
                     /locus_tag="NBH32_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1514092.1"
                     /translation="MKKSLTVTVSSVLAFLALNNAAHAQQHGTQVKTPVQHNYVSNVQ
                     AQTQSPTTYTVVAGDSLYKIALEHHLTLNQLYSYNPGVTPLIFPGDVISLVPQNKVKQ
                     TKAVKSPVRKASQAKKVVQQPVQQASKKVVVKQAPKQAVAKTVNVAYKPAQVQKSVPT
                     VPVAHNYNKSVANRGNLYAYGNCTYYAFDRRAQLGRSIGSLWGNANNWNYAAKVAGFK
                     VDKTPEVGAIFQTAAGPYGHVGVVESVNPNGTITVSEMNYAGFNVKSSRTILNPGKYN
                     YIH"
     gene            28857..29507
                     /locus_tag="NBH32_000461"
     CDS             28857..29507
                     /locus_tag="NBH32_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1514093.1"
                     /translation="MGVHQYFKRLSDMERLIRLPGKFKYFEHNVAAHSFKVTKIAQYL
                     ATVEEYHGRKINWKSLYEKALNHDFAEVFTGDIKTPVKYASSELKKLFSQVEEEMVET
                     FIEEEIPLQYRDVYKQRLQEGKDDSLEGQILSVADKIDLLYETFGEIQKRNPEELFFE
                     IYEMSLETIIQFDHLASVQDFINNIIPEMLTENFIPRTELRETTMNILNKRKEENE"
     gene            29504..29740
                     /locus_tag="NBH32_000462"
     CDS             29504..29740
                     /locus_tag="NBH32_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbA family protein"
                     /protein_id="HCD1514094.1"
                     /translation="MIWYFSAAFFPCVLVVLFSVITRSKWVGTILTLILIGASIYKEY
                     FHNEWIIFIDVVSLLAGYLIIDQLEFHKHQDEDR"
     gene            30009..31994
                     /gene="uvrB"
                     /locus_tag="NBH32_000463"
     CDS             30009..31994
                     /gene="uvrB"
                     /locus_tag="NBH32_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002473941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HCD1514095.1"
                     /translation="MVEHYPFKIHSDFEPQGDQPQAIKEIVEGIKAGKRHQTLLGATG
                     TGKTFTMSNVIKEVGKPTLIIAHNKTLAGQLYSEFKEFFPENRVEYFVSYYDYYQPEA
                     YVPSTDTFIEKDASINDEIDQLRHSATSALFERDDVIIIASVSCIYGLGNPEEYKDLV
                     VSVRVGMEMDRSELLRKLVDVQYTRNDIDFQRGTFRVRGDVVEIFPASKEELCIRVEF
                     FGDEIDRIREVNYLTGEVLKEREHFAIFPASHFVTREEKLKVAIERIEKELEERLKEL
                     RNENKLLEAQRLEQRTNYDLEMMREMGFCSGIENYSVHLTLRPLGSTPYTLLDYFGDD
                     WLVMIDESHVTLPQVRGMYNGDRARKQVLVDHGFRLPSALDNRPLKFEEFEEKTKQLV
                     YVSATPGPYEIEHTDKMVEQIIRPTGLLDPKIEVRPTENQIDDLLSEIQTRVERNERV
                     LVTTLTKKMSEDLTTYMKEAGIKVNYLHSEIKTLERIEIIRDLRMGTYDVIVGINLLR
                     EGIDIPEVSLVVILDADKEGFLRSNRSLIQTIGRAARNDKGEVIMYADKMTDSMKYAI
                     DETQRRREIQMKHNEKHGITPKTINKKIHDLISATVENDENNDKAQTVIPKKMTKKER
                     QKTIDNIEKEMKQAAKDLDFEKATELRDMLFELKAEG"
     gene            32002..34848
                     /gene="uvrA"
                     /locus_tag="NBH32_000464"
     CDS             32002..34848
                     /gene="uvrA"
                     /locus_tag="NBH32_000464"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCD1514096.1"
                     /translation="MKEPSIVVKGARAHNLKDIDIELPKNKLIVMTGLSGSGKSSLAF
                     DTIYAEGQRRYVESLSAYARQFLGQMDKPDVDTIEGLSPAISIDQKTTSKNPRSTVAT
                     VTEIYDYIRLLYARVGKPYCPNHNIEIESQTVQQMVDRIMELEARTKIQLLAPVIAHR
                     KGSHEKLIEDIGKKGYVRLRIDGEIVDVNDVPTLDKNKNHTIEVVVDRLVVKDGIETR
                     LADSIETALELSEGQLTVDVIDGEDLKFSESHACPICGFSIGELEPRMFSFNSPFGAC
                     PTCDGLGQKLTVDVDLVVPDKDKTLNEGAIEPWIPTSSDFYPTLLKRVCEVYKINMDK
                     PFKKLTERQRDILLYGSGDKEIEFTFTQRQGGTRKRTMVFEGVVPNISRRFHESPSEY
                     TREMMSKYMTELPCETCHGKRLSREALSVYVGGLNIGEVVEYSISQALNYYKNIDLSE
                     QDQAIANQILKEIISRLTFLNNVGLEYLTLNRASGTLSGGEAQRIRLATQIGSRLTGV
                     LYVLDEPSIGLHQRDNDRLINTLKEMRDLGNTLIVVEHDDDTMRAADYLVDIGPGAGE
                     HGGQIVSSGTPQKVMKDKKSLTGQYLSGKKRIDVPEYRRPASDRKISIRGARSNNLKG
                     VDVDIPLSIMTVVTGVSGSGKSSLVNEVLYKSLAQKINKSKVKPGLYDKIEGIDQLDK
                     IIDIDQSPIGRTPRSNPATYTGVFDDIRDVFAQTNEAKIRGYQKGRFSFNVKGGRCEA
                     CKGDGIIKIEMHFLPDVYVPCEVCDGKRYNRETLEVTYKGKNIADILEMTVEEATQFF
                     ENIPKIKRKLQTLVDVGLGYVTLGQQATTLSGGEAQRVKLASELHKRSTGKSIYILDE
                     PTTGLHVDDISRLLKVLNRLVENGDTVVIIEHNLDVIKTADYIIDLGPEGGSGGGTIV
                     ATGTPEDIAQTKSSYTGKYLKEVLERDKQNTEDK"
     gene            35340..36272
                     /locus_tag="NBH32_000465"
     CDS             35340..36272
                     /locus_tag="NBH32_000465"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HCD1514097.1"
                     /translation="MLTTEKLVETLKLDLIAGEEGLSKPIKNADISRPGLEMAGYFSH
                     YASDRIQLLGTTELSFYNLLPDKDRAGRMRKLCRPETPAIIVTRGLQPPEELVEAAKE
                     LNTPLIVAKDATTSLMSRLTTFLEHALAKTTSLHGVLVDVYGVGVLITGDSGIGKSET
                     ALELVKRGHRLVADDNVEIRQINKDELIGKPPKLIEHLLEIRGLGIINVMTLFGAGSI
                     LTEKRIRLNINLENWNKQKLYDRVGLNEETLSILDTEITKKTIPVRPGRNVAVIIEVA
                     AMNYRLNIMGINTAEEFSERLNEEIIKNSHKSEE"
     gene            36278..37117
                     /locus_tag="NBH32_000466"
     CDS             36278..37117
                     /locus_tag="NBH32_000466"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCD1514098.1"
                     /translation="MGIVFNYIDPVAFNLGPLSVRWYGIIIAVGILLGYFVAQRALVK
                     AGLHKDTLVDIIFYSALFGFIAARIYFVIFQWPYYAENPGEIIKIWHGGIAIHGGLIG
                     GFIAGVIVCKVKNLNPFQIGDIVAPSIILAQGIGRWGNFMNHEAHGGPVSRAFLEQLH
                     LPNFIIENMYINGQYYHPTFLYESIWDVAGFIILVNIRKHLKLGETFFLYLTWYSIGR
                     FFIEGLRTDSLMLTSNIRVAQLVSILLILISISLIVYRRIKYNPPLYSKVGALPWPTK
                     KVK"
     gene            37125..37610
                     /locus_tag="NBH32_000467"
     CDS             37125..37610
                     /locus_tag="NBH32_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1514099.1"
                     /translation="MRKFLSKTHHHTNPLWRVYRLVKFSKVFKNVIIIEFSKFIPSMV
                     LKRHIYKQLLNINIGNQSSIAYKVMLDIFYPELITIGSNSVIGYNVTILTHEALVDEF
                     RYGPVTIGSNTLIGANATILPGITIGDNVKVAAGTVVSKDIPDNGFAYGNPMYIKMIR
                     R"
     gene            37618..39057
                     /locus_tag="NBH32_000468"
     CDS             37618..39057
                     /locus_tag="NBH32_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1514100.1"
                     /translation="MAQKNNNVIPMTFDDAFYRKMANQKFKQREYKRAAEYFEKVLEL
                     SPDDLEIQIDYAQCLVQLGIAKKAEHLFYDNIIYNRHLEDSFYELSQLNIEVNEPNKA
                     FLFGINYVIVSDDQDYRDELDQMFDVKYQSEEQIELEAQLFVVQILFQYLFSQGRLKD
                     AKNYVLHQPQEVQDHRVVRNLLAMCYLYLGEYDTAKALYEALLQEDSTDIYALCHYTL
                     LLYNTKENEQYQKYLKILNKVVPMNDDESFKLGIVLSYLKQYRASQQLLYPLYKKGKF
                     LSIQMYNALAYNYYYLGEEDESHYYWDKLKQISKVEIGHAPWVIENSKEVFDQHILPL
                     LQSDDSHYRLYGIFLLDQLNGKEIVMTESIWQVLENLNNYEKLYLTYLVQGLTLNKLD
                     FIHRGLLTLYHNELFVSENDVMVAWINQGELIIAEKVDLTDVEPYIGAFIYLYFKNQP
                     RNVTKNQITTWLGITQYKLNKMIEFLLSI"
     gene            39124..40059
                     /gene="trxB"
                     /locus_tag="NBH32_000469"
     CDS             39124..40059
                     /gene="trxB"
                     /locus_tag="NBH32_000469"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCD1514101.1"
                     /translation="MTEIDFDIAIIGAGPAGMTAAVYASRANLKTVMIERGIPGGQMA
                     NTEEVENFPGFEMITGPDLSTKMFEHAKKFGAVYQYGDIKSVEDKGEYKVINFGNKEL
                     TAKAVIIATGAEYKKIGVPGEQELGGRGVSYCAVCDGAFFKNKRLFVIGGGDSAVEEG
                     TFLTKFADKVTIVHRRDELRAQRILQDRAFKNDKIDFIWSHTLKSINEKDGKVGSVTL
                     TSTKDGSEETHEADGVFIYIGMKPLTAPFKDLGITNDVGYIVTKDDMTTSVPGIFAAG
                     DVRDKGLRQIVTATGDGSIAAQSAAEYIEHLNDQA"
BASE COUNT        14490 a         5636 c         7358 g        12835 t
ORIGIN      
        1 ccatataagc tactattttc tcaaatataa atctatgcaa ttggtttaca tttgagaaaa
       61 taagtagctt cattatagtt aatacaatgc tgagataacc atagtaacca tgttgttaaa
      121 gcatttttta attgtaatga ctactttatt taaaagggtt gaagaaagaa ggtgatccaa
      181 tgaaaataat atatttttca tttactggaa atgtccgtcg tttcattaag agaacagaac
      241 ttgaaaatac gcttgagatt acagcagaaa attgtatgga accagttcat gaaccgttta
      301 ttatcgttac tggcactatt ggatttggag aagtaccaga acccgttcaa tcttttttag
      361 aagttaatca tcaatacatc agaggtgtgg cagctagcgg taatcgaaat tggggactaa
      421 atttcgcaaa agcgggtcgc acgatatcag aagagtataa tgtcccttta ttaatgaagt
      481 ttgagttaca tggaaaaaac aaagacgtta ttgaatttaa gaacaaggtg ggtaatttta
      541 atgaaaacca tggaagagaa aaagtacaat catattgaat taaataatga ggtcactaaa
      601 cgaagagaag atggattctt tagtttagaa aaagaccaag aagctttagt agcttattta
      661 gaagaagtaa aagataaaac aatcttcttc gacactgaaa tcgaacgttt acgttattta
      721 gtagacaacg atttttattt caatgtgttt gatatttata gtgaagcgga tctaattgaa
      781 atcactgatt atgcaaaatc aatcccgttt aattttgcaa gttatatgtc agctagtaaa
      841 tttttcaaag attacgcttt gaaaacaaat gataaaagtc aatatttaga agactataat
      901 caacacgttg caattgttgc tttataccta gcaaatggta ataaagcaca agctaagcaa
      961 tttatttctg ctatggttga acaaagatat caaccagcga caccaacatt tttaaacgca
     1021 ggccgtgtgc gtcgtggtga gctagtgtca tgtttcttat tagaagtgga tgacagctta
     1081 aattcaatta actttattga ttcaactgca aaacaattaa gcaaaattgg tggcggcgta
     1141 gcaattaact tatctaaatt gcgtgcacgt ggtgaagcga ttaaaggaat taaaggcgta
     1201 gcgaaaggtg ttttacctat tgctaagtca cttgaaggtg gctttagcta tgcagatcaa
     1261 ctaggtcaac gccctggtgc tggtgctgtg tacttaaata tcttccatta tgatgtagaa
     1321 gaatttttag atactaaaaa agtaaatgcg gatgaagatt tacgtttatc tacaatatca
     1381 actggtttaa ttgttccatc taaattcttc gatttagcta aagaaggtaa agacttttat
     1441 atgtttgcac ctcatacagt taaagaagaa tacggtgtta cattagacga tattgattta
     1501 gaaaaatatt atgatgatat ggttgcaaat ccaaatgttg agaaaaagaa aaagaatgca
     1561 cgtgaaatgt tgaatttaat tgcgcaaaca caattacaat caggctatcc atatttaatg
     1621 tttaaagata atgctaatag agtgcatccg aattcaaata ttggacaaat taaaatgagt
     1681 aacttatgta ctgaaatttt ccaattacaa gaaacttcaa ttattaacga ttatggtatt
     1741 gaagacgaaa ttaaacgtga tatttcttgt aacttaggtt cattaaacat tgtaaatgta
     1801 atggaaagcg gaaaattcag agattcagtt cactctggta tggacgcatt aactgttgta
     1861 agtgatgtag caaatattca aaatgctcca ggagttagaa aagctaatag tgaattacat
     1921 tcagttggtc ttggtgtcat gaatttacac ggttacctag caaaaaataa aattggttat
     1981 gagtcagaag aagcaaaaga ttttgcaaat atcttcttta tgatgatgaa tttctactca
     2041 atcgaacgtt caatggaaat cgctaaagag cgtggtatca aatatcaaga ctttgaaaag
     2101 tctgattatg ctaatggcaa atatttcgag ttctatacaa ctcaagaatt tgaacctcaa
     2161 ttcgaaaaag tacgtgaatt attcgacggt atggttattc ctacttctga ggattggaag
     2221 aaactacaac aagatgttga acaatatggt ttatatcatg catatagatt agctattgct
     2281 ccaacacaaa gtatttctta tgttcaaaat gctacaagtt ctgtaatgcc aatcgttgat
     2341 caaattgaac gtcgtactta tggtaatgcg gaaacatttt accctatgcc attcttatca
     2401 ccacaaacaa tgtggtacta caaatcagca ttcaatactg atcagatgaa attaatcgat
     2461 ttaattgcga caattcaaac gcatattgac caaggtatct caacgatact ttatgttaat
     2521 tctgaaattt ctacacgtga gttagcaaga ttatatgtat atgcgcacta taaaggatta
     2581 aaatcacttt actatactag aaataaatta ttaagtgtag aagaatgtac aagttgttct
     2641 atctaacaat taaatgttga aaatgacaaa cagctaatca tctcgtttaa aataacagat
     2701 gattagtctg ccatgtctat atttgtcaat tattgagagt aacattacag gaggaaatta
     2761 tattcatgat agctgttaat tggaacacac aagaagatat gacgaatatg ttttggagac
     2821 aaaatatatc tcaaatgtgg gttgaaacag aatttaaagt atcaaaagac attgcaagtt
     2881 ggaagacttt gtcagaagct gaacaagaca catttaaaaa agcattagct ggtttaacag
     2941 gcttagatac acatcaagca gatgatggta tgcctttagt catgctacat acgactgact
     3001 taagaaaaaa agcagtttat tcatttatgg cgatgatgga gcaaatacac gcgaaaagtt
     3061 attcgcatat tttcacaacg ttattaccat ctagtgaaac aaactattta ttagatgaat
     3121 gggttttaga agaaccacat ttaaaatata aatcagataa aattgttgct aattaccaca
     3181 aactttgggg taaagaagct tccatatatg accaatatat ggccagagtt acgagtgtat
     3241 ttttagaaac attcttattc ttctctggtt tctattatcc gctatattta gctggtcaag
     3301 ggaaaatgac gacatcagga gaaatcattc gtaaaattct tttagatgaa tctattcatg
     3361 gtgtgtttac tggtttagat gcacagcatt tacgaaatga actatctgaa agtgagaagc
     3421 aaaaagctga tcaagaaatg tataaattgc taaatgactt gtatttaaat gaagagtcat
     3481 acacaaaaat gttatacgat gatcttggaa tcactgaaga tgtgttaaac tatgttaaat
     3541 ataatggaaa taaagcactt tcaaatttag gttttgaacc ttattttgag gaacgtgaat
     3601 ttaacccaat cattgagaat gccttagata caacaactaa aaaccatgac ttcttctctg
     3661 taaaaggtga tggttatgta ttagcattaa atgtagaagc attacaagat gatgactttg
     3721 tatttgacaa caaataataa ttaaaattaa aaagaccttc acatgaaagg gcaaagcgat
     3781 gacattcgct tccgacatgt tgaaggtctt ttctctatgc ttaaaaacgt atgcacattt
     3841 tgaaaaagta aaaattgcat ttaatgatgt caatgaggct agaaagtata caagatattg
     3901 cattacaaaa gggtgtgcta ttttactata tacatctaaa atacacattt acaaaaattg
     3961 atttctattt taattgcaaa tatcaataaa atcgacaata aattatttga aaggctattg
     4021 aaatgagtga caaaaaacga taccattaat gagaaatgtt atcaatgata ataattatca
     4081 ttaaattaaa gggagaaaat tttgtaatga agtatttatt aaagggaaaa attttgcttc
     4141 tattacttat attgctaaca atcatttcgt tgtttatagg tgtcagtgaa ctgtctatta
     4201 aagatatctt tcatttatca gactcacagc aaaatatatt gttctcaagc cgaataccga
     4261 gaacgatgag tattttaatt gctggtagtt cattagcttt agctggccta atcatgcaac
     4321 aaatgatgca aaataagttt gttagtccaa ctacagctgg gacgatggag tgggcaaaat
     4381 taggtatctt aattgcctta ttattctttc caactggtca tattttatta aaattagtat
     4441 ttgctgttat ttgcagcatc ggcggaacat ttttatttgt taaaattatt gattttatca
     4501 aagtgaaaga tgtcatcttt gtaccactat tagggattat gatgggtggc attgttgcta
     4561 gttttacaac gtttatctca ttacgtacga acgcagttca aagcattggt aactggctta
     4621 acgggaactt tgccattatc acaagtggac gttatgaaat cttgtattta agtattcctc
     4681 ttttagcatt gacatatctt tttgcgaatc atttcacaat tgtaggaatg ggtaaagact
     4741 ttaccaataa tttaggtttg agttacgaaa aattaattaa catcgcattg tttattactg
     4801 caactattac agcattggta gtggtgactg ttggaacatt accgttctta ggactagtaa
     4861 taccaaatat tatttcgatt tatagaggcg atcatttaaa aaatgctatc cctcatacga
     4921 tgatgttagg tgccatcttt gtattgtttt ctgatatagt tggcagaatt gtagtttatc
     4981 cttatgaaat taatattggt ttaacaatag gtgtatttgg aacaatcatt ttccttatct
     5041 tacttatgaa aggtaggaaa aattatgcgc aacaataata aaaaaataat gcttttaatt
     5101 gtagtcacgt tcttaattag tatgctgtac ttatttgttg gtattgattt tgaaatattt
     5161 gaatatcaat tttcaaatcg tttaagaaag ttcatattaa ttattttagt aggtgctgcc
     5221 attgcaacat ccgttgttat tttccaagcg attacaaata atcgtttatt gacaccatca
     5281 ataatgggat tagatgcagt ttatttattt atcaaagtat tgccagtctt tttatttgga
     5341 attcaatcgg tgtgggttac taatgtatat ttgaatttta tattaacact tataacgatg
     5401 gtgttattcg cactaatcct attccaaggt atctttaaaa ttggacattt ttcaatttat
     5461 tttatcttac ttattggtgt ccttttagga acatttttta gaagcattac aggttttatt
     5521 caattgatta tggatcctga atcattttta gcaatacaaa gtagtatgtt tgctaatttt
     5581 aatgcctcta attcgaattt agtttctttt tcagcagtat tattagtaat cttattagtc
     5641 attacaattt ttctattgcc gtatttagat gtattgcttt taggtcgtgc tgaagcaatt
     5701 aatcttggta tatcgtatga aaaattaacg cggattctac ttgtaatagt ttctgtctta
     5761 gtttctgtgt caactgcatt agtaggacca attacatttt taggcttatt gactgtaaat
     5821 ctagcgcatg aactaatgaa gacgtatgaa cataaatata tattaatcgc gacaatttgc
     5881 ttgagttgga ttagtttatt tagtgcgcaa tgggtagttg aaaatgtgtt tgaggctacg
     5941 acagaaatga gtatacttat tgatttaatt ggtggaagtt atttcattta tctattagtt
     6001 agaaggagaa atgcgcaatg attcaagttg gaaatttaac taaaactata aataatcaaa
     6061 tgatattgga cgatattagc atagaaattg aaaaaggtaa gttgacttct ttaattggac
     6121 ctaatggagc agggaagagt acgttacttt cagcgatatg tcgcttaatt cggtttgatg
     6181 aaggggaagt aaaaatagat ggacagctta tgtctgatta taaaaacaat gatttgtcga
     6241 aaaagatatc gattttaaag caaacgaacc atacagaaat gaacattact gtagaacaat
     6301 tagttaattt cggacgattc ccttattcta aaggacgttt aacgaaagaa gatcatgaaa
     6361 ttgttaatga tgctttagat ttgttgcaat tacaagatat tagaaagcgt aatataaaat
     6421 cattgtctgg tggacaacgt caacgtgcct atatagcgat gaccattgca caagatactg
     6481 aatatattct tttagatgaa ccgttaaata atttagatat gaagcatgct gttcaaatta
     6541 tgcaaacgtt aaaaatgtta gcacataaaa tgaataaagc gattgtcatt gtgttacatg
     6601 atattaactt tgcgtcctgt tattcagatc aaattgtagc actgaaaaac ggacaactag
     6661 ttaaatcaga tttgaaagat aatgtcattc aaagtagtgt tttaagtgat ttatatgaca
     6721 tggatattca aattgaacat ataaaaaatc aaaggatttg tttatatttt aaggattgat
     6781 aattcggaaa cactttaaag gggtgatgca ccaattaaag aaagtttaac tataaatcat
     6841 ttatttattt ttcacatcaa gcacacagat taagccaaaa gaggagaaaa ttatattatg
     6901 aagaaaacag tcttatattt agtagtagca gtaatgtttt tattagcagc atgtggtaac
     6961 aattctgata aagaacaatc aaaatcagaa actaaaggtt ctaaagatac agtgaaaatt
     7021 gaaaacaact ataaaatgcg tggcgagaaa aaagatggta gcgatgctaa aaaagttaaa
     7081 gaaactgttg aagtaccaaa aaatcctaaa aatgcagttg tattagatta tggtgcatta
     7141 gatgtaatga aagaaatggg cttatcagac aaagtaaaag cattacctaa aggggaaggc
     7201 ggcaagtcat taccgaactt cttagagtca tttaaagatg acaagtatac aaatgttggt
     7261 aacttgaaag aagttaattt cgataaaatt gcagcaacaa aaccagaagt aatctttatc
     7321 tcaggccgta cagctaatca aaagaattta gatgaattca aaaaagctgc accaaaagca
     7381 aaaattgttt atgttggtgc agatgaaaag aacttaattg gttcaatgaa acaaaacact
     7441 gaaaatatcg gtaaaattta cgacaaagaa gataaagcta aagaattaaa taaagattta
     7501 gataacaaaa ttgcctcaat gaaagataaa actaagaagt tcaacaaatc agttatgtat
     7561 ttattagtta atgaaggtga attatctact tatggaccta aaggtcgttt cggtggatta
     7621 gtttacgata cattaggttt caacgcggtt gataaaaaag taagcaatag taaccacgga
     7681 caaaatgtct ctaacgaata cattaataaa gaaaatccag atgttatttt agcaatggat
     7741 agaggacagg ctgtaagtgg taaatcaact gcgaaacaag cattaaataa tcctgtatta
     7801 aaaaatgtta aagcaattaa agaagacaaa gtttataatt tagatcctaa attatggtac
     7861 tttgcagctg gatcaactac aactacaatt aaacaaattg aggaacttga aaaagttgta
     7921 aaataatttt aaaagtgggg aacaatggtt aaaggtctta atcattgttc cccacttttc
     7981 ttttaaaaaa ggaatctggg acgttaatca atgtcctaga ctctaaaatg ttctgttgtt
     8041 tgtcgttggt tgaatgaaca tgtacttgta acaagttcat ttcaatacca gtgggctccc
     8101 aacatagaga aatttgattt ccaatttcta ctgacaatgc aagttggcgg ggcccaaaca
     8161 tagagaatat caaaaaggaa ttctatagaa gtggtgcttt atcatgtctg tcccactccc
     8221 tataatgttt tggctatgtt gtttaaattt cgaaataaat atgataatga tatttgcagc
     8281 gattgttaaa tcgagattga caatttggac aacgttcaac catcatatat tcattgattg
     8341 ttaattcgtg tttgcataca ccgcataaga ttgctttttc gttaaatgaa ggctcagacc
     8401 aacgcttaat ggcgtgcttt tcaaactcat tatggcactt atagcatgga tagtatttat
     8461 tacaacattt aaatttaata gcaataatat cttcttcggt aaaataatgg cgacagcgtg
     8521 tttcagtatc gattaatgaa ccataaacta taggcataga caaagctcct taacttacga
     8581 ttcctttgga tgttcaccaa taatgcgaac ttcacgattt aattcaatgc caaatttttc
     8641 tttgacggtc ttttgtacat aatgaataag gttttcgtaa tctgtagcag ttccattgtc
     8701 tacatttacc ataaaaccag cgtgtttagt tgaaacttca acgcctccaa tacggtgacc
     8761 ttgcaaatta gaatcttgta tcaatttacc tgcaaaatga ccaggcggtc tttggaatac
     8821 actaccacat gaaggatact ctaaaggttg tttagattct ctacgttctg ttaaatcatc
     8881 cattttagct tgtatttcag tcattttacc aggagctaaa gtaaatgcag cttctaatac
     8941 aactaagtgt tctttttgaa taatgctatt acgataatct aactctaatt cttttgttgt
     9001 aagtttaatt aatgagcctt gttcgtttac gcaaagcgca tagtctatac aatctttaac
     9061 ttcgccacca taagcgccag cattcatata cacagcacca ccaattgaac ctggaatacc
     9121 acatgcaaat tcaaggccag taagtgcgta atcacgagca acacgtgaga catcaataat
     9181 tgcagcgccg ctaccggcta ttatcgcatc atcagatact tcgatatgat ctagtgataa
     9241 taaactaatt acaatgccac gaataccacc ttcacggata ataatatttg agccattgcc
     9301 taaatatgta acaggaatct cattttgata ggcatattta acaactgctt gtacttcttc
     9361 atttttagta ggggtaatat aaaagtcggc attaccacct gtcttagtat aagtgtatcg
     9421 ttttaaaggt tcgtcaactt taattttttc atttgggata agttgttgta aagcttgata
     9481 gatgtcttta tttatcactt ctcagtacat cctttctcgt gtctttaata tcatatagta
     9541 ttataccaat tttaaaattc atttgcgaaa attgaaaaga aagtattaga attagtataa
     9601 ttataaaata cggcactatt gtcgttataa gtatttttta catagttttt caaagtattt
     9661 ttgcttttgc atctcatatt gtctaattgt aaagctatgt tgcaatattt gatgcttttt
     9721 agtattgaat tgtaaagcaa tctcatcatt agtcgataag agataatcaa gtgcaagata
     9781 agattcaaat gtttgggtat tcatttgaat gatatgtaga cgcacctgtt gttttagttc
     9841 atgaaaatta ttaaacttcg ccatcataac tttcttatta tatttatgat gcaagcgata
     9901 aaacccaaca taatttaaac gtttttcatc taaggatgta atatcatgca aattttctac
     9961 acctactaaa atatctaaaa ttggctctgt tgaatattta aaatgatgcg taccaccaat
    10021 atgttttgta tattttactg ggctgtctaa gaggttgaat aataatgatt caatttcggt
    10081 gtattgtgat tgaaaacaat tagttaaatc actattaatg aatggttgaa catttgaata
    10141 catgataaac tcctttgata ttgaaaatta atttaatcac gataaagtct ggaatactat
    10201 aacataattc attttcataa taaacatgtt tttgtataat gaatctgtta aggagtgcaa
    10261 tcatgaaaaa aattgttatt atcgctgttt tagcgatttt atttgtagta ataagtgctt
    10321 gtggtaataa agaaaaagag gcacaacatc aatttactaa gcaatttaaa gatgttgagc
    10381 aaaaacaaaa agaattacaa catgtcatgg ataatataca tttgaaagaa attgatcatc
    10441 taagtaaaac tgatacaact gataaaaata gtaaagaatt taaggcacta caagaagatg
    10501 ttaaaaacca tctcatacct aaatttgaag catattataa gtcagcaaaa aatttgcctg
    10561 atgatacaat gaaagttaag aaattaaaaa aagaatatat gacgcttgca aatgagaaga
    10621 aggatgcgat atatcaatta aaaaaattca taggtttatg taatcaatct atcaagtata
    10681 acgaagacat tttagattat acgaaacaat ttgaaaaaaa tagatacaaa gttgaatcag
    10741 aaattaaatt agctgataat aaaagtgaag caactaatct tacgacaaaa ttagaacata
    10801 ataataaagc gttaagagat actgcgaaga agaacctaga tgatagtaaa gaaaatgaag
    10861 taaaaggcgc gattaaaaat cacattatgc caatgattga aaaacaaatt accgatatta
    10921 accaaactaa tattagtgat aagcatgtta ataatgcaag gaaaaacgca atagaaatgt
    10981 attacagtct gcagaactat tataatacac gtattgaaac aataaaggtt agtgagaagt
    11041 tatcaaaagt cgatgtagat aagttgccga aaaagggtat agatataact cacggcgata
    11101 aagcctttga aaaaaagctt gaaaaattag aagaaaaata actataatca tttttcaaag
    11161 ttaaaaattt tgaatttatg gttaacatgt caacttacta tgtgtataat ggtaaacatt
    11221 gatattaact atatgtataa aaatgtcacg cagatgctat ttaaatgtga taaatatttt
    11281 tagaggtgaa tagagtggct ataaagctaa gttcaattga ccaatttgaa caggttattg
    11341 aggaaaataa atatgttttt gtattaaaac atagtgaaac ttgtccaata tcggcaaatg
    11401 cgtacgatca atttaataaa tttttatatg aacgcgatat ggacggttat tatttgattg
    11461 tccaacaaga acgcgatttg tcagattata ttgctaaaaa aacgaatgtt aaacatgaat
    11521 cacctcaagc attttatttt gtaaatggtg aaatggtttg gaatcgagac cacggtgata
    11581 ttaatgtgtc gtcattagca caagcagaag aataatgaaa atatagggtt ggaacatttt
    11641 gccttacact actagacgtg aatagcacaa cttaaattcg tgtgaatcag ggtagttcgg
    11701 ctataatgat gttctgacct tttatttttt gtaacttata gaagtagtta agttagtact
    11761 tttttacaaa catatgtata atgtattcga gtatttttat tgaaaatatt ttggaaaacg
    11821 acgaatccaa taagaaaatt taaacatgat ttgtaagtta gtttaatagg aaatacatgc
    11881 taagccagaa gaaacatact gttatttact ggaataatta ataatcatgt catgtaaaat
    11941 gttcgcatat aatcacgaga taaaatctaa aatttaagat taatctttta agaataaaaa
    12001 acgtatcaca acaaataata aagtaaggtg gtcaaggtta tgaaagtatt agtagccatg
    12061 gatgagtttc atggaattat ttcgagttat caagctaata gatatgttga agaggcagtt
    12121 gcaagccaaa ttgaaactgc agatgtagtt caagtaccat tgtttaatgg aagacatgaa
    12181 ttattagatt ctgtattttt atggcaatct gggcaaaagt atcgtatacc agtacatgat
    12241 gcagatatga atgaagttga aggtgtttac ggacaaactg atacagggat gaccgttatc
    12301 gaggggaatt tatttttaaa aggtaaaaaa ccaattgttg aacgaacaag ttatggttta
    12361 ggagaaatga ttaaacatgc attagataac gacgcaaaac atgttgtaat ttcactaggt
    12421 gggattgata gttttgatgc tggtgcaggt atgttacaag cattaggtgc tcaattctat
    12481 gatgacgaag ggcgtgtcgt agatatgaga caaggtgctg gtgtaattaa atatattcgt
    12541 cgtatggata tgtcaaactt acaccctaaa atggaaacag caagaattca agtaatgtcg
    12601 gatttttcaa gtcgattata tggtaagcaa agtgaaatca tgcaaactta tgatgcgcat
    12661 cagttgaatc ataatcaagc agcagaaatc gataatttaa tttggtattt tagtgagtta
    12721 tttaaaagtg aattgaaaat tgcaattggt ccagttgaac gtggtggtgc tggtggtgga
    12781 attgcagcag tcttgaatgg actgtatcaa gctgaaatat taaccagtca tgcattagta
    12841 gaccaactaa cacatttaga aaatttagtt gaacaagcgg atttaattat ttttggagaa
    12901 ggattaaacg aaaatgatca gttgctagaa acgacaacat tgcgtattgc agaactttgt
    12961 cataaacatc aaaaggttgc cattgcaatt tgtgcaactg ctgaaaagtt tgatttattt
    13021 gaatcacaag gggttacagc aatgtttaat acctttatcg atatgccgga aagttatact
    13081 gactttaaaa tgggattaca aattaggcat tatactgttc agtctttaaa actgttgaaa
    13141 acacatttta atgttgaggt ttagtaaaga aggactaaat tggtgatgct gtcatgatgg
    13201 ttaataacat ttatgatggt tagcaaaacg aattagaaga tcgaaagtat acgtaaaaaa
    13261 tatgaaaaat cacgctatca ttgcactgaa tgttagcgtg attttttata ttaattaagc
    13321 ttgagttgaa ctagtatata atcgtttgtt tttagtgatt ttcagcgata tcttctacaa
    13381 ttccaatgat tacttgtact gctttttcca tgacatcaat ggatgcatat tcatatgggc
    13441 cgtggaagtt accgcaacct gtaaagatgt ttggagttgg taaccccata aatgacaatt
    13501 gtgaaccatc tgtaccaccg cgaataggtt cagtgtttgc tggaatatct aatttggcaa
    13561 agacacgttt aggtatatca ataatatgag gcaatggtaa tattttttct gccatattga
    13621 aatattgatc cgatatatca actttaactg gataattttc aaaatgggca ttgatatcgt
    13681 cacgtatttc taaaatacgt ttcttacgca attcgaattg ttttttatca tgatcacgaa
    13741 taatgtattg caaagttgct ttttcaacag ttccttcaaa gttcattaag tgataaaagc
    13801 cttcgtatcc ttctgttcgc tccggaactt cactatcagg tagcaaacta tcgaattgtt
    13861 cacctaaacg tattgcgttt accattgcat ttttagctga accaggatgt acattcacac
    13921 cgtggcatgt aataatcgct tcagcagcgt taaagctttc atattgtaat tctccatatt
    13981 gactaccatc catagtataa gcaaaatcgg cattgaagcg gtcaacatca aatttatgtg
    14041 gaccacgacc gatttcttcg tctggtgtaa atccaatgcg aatggtacca tgtttaattt
    14101 ctggatgttc ttgtaaataa caaatagctt ccataatttc cacaataccc gctttatcgt
    14161 ctgcacctag taacgatgta ccatcagtta ccattaatgt atgaccaact aaactgttaa
    14221 gttctggaaa tactttagga tctaagacac gtttagtatt gcctagtttg tatggcttac
    14281 catcatagtt ttcaataatt tgcagtttaa catttgaagc attgaaatca ggtgatgtat
    14341 caacatgcgc caaaaatcca actgttggga cgtcgacatc gatgttactt tctaatgtag
    14401 caaataagta gccattttca tctaaatcag ttggcaatcc taattgttgt aattcttttt
    14461 ctaataaatg taacaaatcc cattgctttt cagttgaagg tgttgttgta gattttggat
    14521 cagattgcgt atcaattgtc gtatatcttg ttaatctatc tatcaattgg ttcttcatta
    14581 tattcgaccc cttaaactct attattcatg ttgtaagatt ttttatatgt cttacctttg
    14641 attttaccat acagttgttt gatacgtgtg tataggtaat atagaatttc agaaactaat
    14701 ataccgaaag caatcgcacc tgaaatcagt gtaacttcta aaaatgtatt tacagcactt
    14761 gtataatcat tcgatactaa aaaacgagtc gcttgataag ctgcaccacc aggtactaat
    14821 ggtataatgc ctggcactat gaatataatt accggtcgtt tatatctgcg actcatagta
    14881 tgactcatta agcctaaaat taagcttccc aaaaatgaag cgccaacttt tccaaactct
    14941 aaatctaccg ttaattggta aatcgtccat gcaatggcac ctacaaatcc acatgctact
    15001 aagagacgtt tgggtgcatt gaaaatgata gagaaaagta ctgttgatat aaagctgatt
    15061 gtaaaatgaa ataaataaaa tagcatgctt taacagtcct tccttaaatg attaataaaa
    15121 cgatagcgac accagcaccg attgcgaatg ctgttaatgc agcttcaaca ccgcgagaca
    15181 tacctgcaag taattcaccc gctaataaat ctcgaatggc attggtaatt aatataccag
    15241 ggacaagtgg catgacactg gctatagtaa tgatatcttg attggttgca atacctaatt
    15301 tagtaaatgt agctgcaatt gatatgacca cagcggctgc aacaaactct gagaaaaatt
    15361 taatttgtat atagcgttgc acaaagctga atgttaaaaa tgcggatcca ccagcaatga
    15421 ctgcaatcca acaatctgat gcgacacctc caaacataaa taggaagaag ccacatgcaa
    15481 tggcagctgc aaagaaattc gttaaaaaag aatattgtaa tgatgcatgc tgtaaatgaa
    15541 taaattcaga tttagcttca tcaattgtga gttctttatt tgatatttta cgtgaaagac
    15601 tattcgttaa agcgattttc tctaaatctg ttgtacgctc ttgtacacga attaatcttg
    15661 tacttgttcg atcgtttaat gaaaaaataa ttgcagttga actgacaaaa ctatatgtat
    15721 tatgaagacc ataactatgt gcgatacggt tcattgtatc ttcaactcga tatgtttcag
    15781 cacctgattc aagtaaaatt ctacctgcaa ttaatacaac atcaatcact ttgttttcat
    15841 ctataattgt gattgaatct ggcatatcaa ttcacctcca atgatatgtg ttatttattt
    15901 gaacaattga agtttacaac ttgttgttgc aactttcaat agtgagactt tatgttagta
    15961 tgatgaactt gtatggttca aatttaaata agaaaaactg ttaatctttg ctattatact
    16021 atgatttaat aatagcaaag gattaacagt tttgtcgttg ttataaattg ataatagggt
    16081 taaacattac tttgtttcgc ccttgatttt tggctacatg caccatatcg tctgcatctt
    16141 taaacacttt acgctgtgat tttggatcgt catctgttaa ataaccaaca ccgatagata
    16201 ctgacaattt aataacttct ttgtttggta aatggaatga tgatttttca acacccgaac
    16261 gaatattttc agctaattta acactttgat caagtgaata attgtgaatg acaactgaga
    16321 actcttcgcc accatttcta aaaattttaa attgattcgg cacatagttt ttaagtaatt
    16381 gagacatttg ttttaataca gcatcacctg atttgtgtga gtaggtatca ttgacatctt
    16441 taaatccatc gatatcgatt aataataatg cgatactttg atgttctttt tcagcttttc
    16501 gtgaaatttc atttaaatgt ctatcaaatt cttttacatt gcctaagcct gttaagtaat
    16561 catatttatc ttcgttttca taacgattta cgagtgagaa gaaatgccaa atatcgacaa
    16621 atgttatcgc agaagctaaa gtgataatta atgaaattgg tattaaaatg ataacttccg
    16681 atagtgtgta aataggactc actaatgcga caccaaataa aatgattatt gtaacaacat
    16741 taagtattaa taatgatagc acatcatttt gttttaaaaa tggtccaata gcacttgtta
    16801 ctgcggcaat tacaatcaac gtaacaccgt acataatcga gttgttaaat actacaattt
    16861 caacaattgc tacaattact gtggcagata atgtatagac catatttgta aatctaccta
    16921 aaaacaataa aggaacgaat gttaagtgaa ttaaataatc ttcacgataa gggatagggt
    16981 agacagataa taataatgat acgattgtca ttaaaacagt gacataagcc ttagaaaaaa
    17041 ccatacgttt gttttctgaa tactgtaagc gatggaataa atagattcca gcgactataa
    17101 cagatatatt gtatataaat gcttcgaaca tgtctgaatc gactccttta attgaccact
    17161 agctattgta agtgaaaact tacaatttgt cattagttta catataaaat taatgtatga
    17221 tatagacttt gatgttaaaa tgttgcctta aatgatatga tgaaaaaatg aataataggc
    17281 gatatataag aaatgaatcg tatagttgta aatatgatat cattaattga gcgaattaat
    17341 ttataataaa gctataagat atacgaagaa aatagatata tcattctata aagacaatat
    17401 taaataaata taacgttaaa aacaattaat atcgatgaag gtgaataaat ggttacatta
    17461 ttactagttg cagtaacaat gattgtcagt ttgacgataa caccaattgt tattgcaata
    17521 tcgaaaagat taaatttagt tgataaacca aattttagaa aagtacacac taaacctatt
    17581 tcagttatgg gtggtacagt gattctcttt tcatttttaa taggtatttg gattggtcat
    17641 cctattgaaa cagaaatcaa accacttatt attggtgcga ttattatgta cgtacttggg
    17701 cttgtagatg atatctacga tttgaaaccg tatataaaat tggctggtca aattgccgct
    17761 gccttagtag ttgcttttta tggtgtgact attgatttta tttcgttgcc aatgggtaca
    17821 acgattcatt ttggatttct tagtattcca attactgtga tttggattgt tgctattaca
    17881 aatgcaatta acttaattga tggactcgat ggtttggcgt cgggtgtttc tgcaatagga
    17941 ctcattacaa taggattcat tgcaatttta caagctaata ttttcataac gatgatttgt
    18001 tgtgttttat taggctcttt aattgggttt ttattttaca atttccatcc tgccaaaata
    18061 tttttaggtg atagtggggc tttaatgatt ggatttatca tcggattcct ttctttgctc
    18121 ggattcaaaa atattacaat tattgcattg ttcttcccaa ttgttatcct agcagtgcca
    18181 ttcattgata ctttgttcgc aatgattcga cgtgtgaaaa aagggcagca tataatgcaa
    18241 gctgataaat cgcatttgca tcataaacta ttagcattag gctacacaca tagacaaaca
    18301 gtattattaa tctattcaat ctctatttta tttagtcttt cgagcattat cttgtatgta
    18361 tcgccaccat taggtgttgt attaatgttt atattaatca tatttagcat tgaattaatt
    18421 gttgaattta caggattaat agataacaac taccgaccaa tattaaattt aattagtcgg
    18481 aaatcgtcac acaaagaaga ttagatttta aaacggtaag taataaaata aaaaatgatg
    18541 tctgtacttt tatgttgtta taaaggaagg catactcaca aaaaacacta gcatggaacg
    18601 tgctttcaaa attgcaaagt aacaatacgc aattagagtt agccatccta agctagtgtt
    18661 tttattttta ttcagttggt atatcgaaag gtaactgctt tggagtttct tcagtcaaat
    18721 cgaaatttcc agcagtcatt tgatttaaaa agttaataaa cgcttcatag tcacttttaa
    18781 cgacatcgat atagtagctt accttatcag tgtatgtttg gtttcttaac ataaaatgag
    18841 ttgaagctaa ttcatattca aatttaccag tttgatcata attcagtgtt actatacatg
    18901 gtactgcttc tcgtagttcg acacgtccga catcataaat gacgtctcta acagcaccgc
    18961 tataggcgcg aattaaaccg ccgccaccta atttaatacc accaaaatat cttgttacta
    19021 cgacacacgc attatgaaca tcgagctttt ttaatatgtc taacattggg acaccggcag
    19081 ttcctgtcgg ttcaccatca tcattcgctt tttgaatatt catttcaggt ccaatagtat
    19141 atgcagaaca attatgagtg gcatctttat gttctttttt tattgcagca ataaatgctt
    19201 tagcttcatc ttcattttga acaggtttga tatgagcaat gaattttgat ttactaatca
    19261 cattttcaat aatgtgttct tttttaacag taatgatatt ttgtgtcata ataactcctt
    19321 aattcataag cttaagatta tttaatcttc attatacact gaaaatgaca tgactataaa
    19381 tcgtttgatt gccattttct ttttaactga aatattgtat cattgttatg agtatatttt
    19441 aggaggacga ctatgaaaat tgctgtgatg accgattcta caagttatct gtcgcaggac
    19501 ttaatcgata aatataatat tcaaatagcg ccattaagtg tgacttttga tgatgggaag
    19561 aactttacag aaagtaatga aatagcaatt gaagaatttt ataataaaat ggcatcgtct
    19621 caaacgattc caacaacaag ccaaccagca attggcgaat ggattactaa atatgaaatg
    19681 ctaagagatc aaggttacac agatatcatt gtcatttgct tatcaagtgg aattagtgga
    19741 agttatcaat ctagttatca agcaggggaa atggttgaag gtgttaatgt acatgcattt
    19801 gatagtaagc ttgcagcaat gattgaaggt tgttatgtat tacgtgctat tgaaatggtt
    19861 gaagaaggat acgaaccaca gcaaattatt gatgatttaa ctaatatgcg tgaacacact
    19921 ggtgcatatt taattgttga tgacttaaag aatttacaaa aaagtggtcg aattactggt
    19981 gctcaagcat gggttggaac attattaaaa atgaagccag ttcttaagtt tgaagatggc
    20041 aagattatac cagaagaaaa agttcgtact aaaaagcgtg ccattcaaac attagaaaag
    20101 aaagtgttag acattgtaaa agactttgaa gaagtaactt tatttgtcat aaatggagat
    20161 cattttgaag atggtcaagc gttatacaaa aagttacaag aagattgtcc ttcaggttat
    20221 caagtagcat actctgagtt tggtccagtt gttgcagcac atttaggttc tggtggatta
    20281 ggtttaggct atgttggcag aaaaataaga ttaacataat tataaaattt taataaaaga
    20341 gtctatattg taattggaaa ttatctcttg catacatggc tttaaatgtt catcatttga
    20401 atgtcaaaat gctaaagata taagaaaatc attataatat tagactcttt tttacgttga
    20461 aatgaggttt taagcattaa acattacggg aaattaattc atcctcatac ttcacttact
    20521 aatgaaaaaa ttaaaaaaga agtaacaggt gtcatcaaac aaaattcaaa ctattattgt
    20581 gttcaatgtg aaagtacaaa tccaaagcat ttttatcagt atgattcctc agtacattcc
    20641 aaaaaaattg tatattgcag aaattgtata tcactgggtc gaatggataa tgtaacaaga
    20701 tataaaataa cagagagttc gcaaagttca tcacaagcct attatcatct ctcatttgaa
    20761 ttgtcggaac agcagtctta tgcctcagaa catattgttc gagccattag aatgagacaa
    20821 acgattttgt tatatgcagt aacaggtgca ggcaagacag aaatgatgtt tcaaggcatt
    20881 caatatgcaa gacgacaggg agataatata gctattgtgt caccacgtgt agatgttgtt
    20941 gtagaaatta gtaaacgtat taaagacgca tttcttaatg aagatataga catactacac
    21001 cagcaatcaa gtcaacaatt tgaagggcat tttgttgtat gcacagtgca tcaactttac
    21061 cgattcaaac agcactttga tactattttt attgatgaag tcgatgcctt tcctttatca
    21121 atggataaaa gtttacaaca agcattgaag tcatcttcta aagttgaaca tgcaacaatt
    21181 tatatgacag caacaccacc gaaacaactt ctgtcagaga ttccccacga aaatgtaatt
    21241 aaattgccag ctcgctttca taaaaaatca cttccagttc ctaaatatcg ctatttcaaa
    21301 ctcaataata ataagattca gaaaatgtta tatcgaattt tacaagatca aattaataaa
    21361 caacgttata cactggtgtt ttttaacaat atagaaacaa tgattaaaac attttcggtt
    21421 tataagcaga aaattactaa attaacatat gtccatagcg aggatgtttt tcgctttgaa
    21481 aaagttgaac aattaaggaa tggacaattt gatgtcattt ttactacgac aatattagaa
    21541 cgtggattta caatggcaaa tttggatgtg gttgttatcg atgcgcatca atatactcaa
    21601 gaggctttaa tacaaattgc tggacgtgtt ggacgaaaat tagaatgtcc tactggaaaa
    21661 gtattgtttt ttcatgaagg agtaagtatg aatatgattc aagctaaaaa agagattcaa
    21721 aagatgaaca aattagcatt aaaaagaggt tggattgatg aataattgtt tgagttgtgg
    21781 tgctaagtta tatgaaaata taaccattta taatttgttc aagaaaccta atagattatg
    21841 tgacagatgc aaagagaatt gggacaatat taaacttgat attaaagcaa ggcgatgttc
    21901 aagatgctta aaatacttaa atcaaaatga agcgtattgt ttagactgca agtttctatc
    21961 ggcacacttt aatttaatgg aacaattata ttgtcaattt caatatgatg gtttaatgaa
    22021 agagatgata catcagtata aatttttgaa agactattat ttatgtgaat tattggcaca
    22081 tttgattgaa ataccacaaa catcttatga ctatattgtg ccaattcctt cttcgccggc
    22141 acatgattta tctagaacat ttaacccggt agaagcagta ctaaaagcta aagggattcg
    22201 ctttgataag attttaaaga tgtcaaatag accaaaacag tctcatttaa ctaagaaaga
    22261 gcgtctggca gatgaaaatc catttattat tgatacggaa ttagatttga acggcaagga
    22321 aatattactc gttgacgata tttatacaac tggattaaca attcatcgtg cagggtgtaa
    22381 attatatgct aaaaatatca gaaaattcaa agtgtttgcg tttgcacgat agcgtaaaaa
    22441 tgttaaaata taataaagag ttaccaataa agaggtttaa ggagagatta ctatgattag
    22501 atttgaaatt catggagata acctcactat cacagatgct attcgcaact atattgagga
    22561 aaaaattggt aagttggaac gttattttaa tgacgtacca aatgcagtgg cgcatgttaa
    22621 agttaaaact tattcaaatt cagctactaa aattgaagta acaattccat tgaaaaatgt
    22681 tacgttaaga gctgaagagc gaaacgatga tttatacgca ggtattgatt taattaataa
    22741 taaacttgaa agacaagttc gaaaatataa aacacgtatt aatcgtaaga gccgtgatcg
    22801 aggagatcaa gaagtgtttg ttgccgaatt acaagaaatg caagaaacac aagttgataa
    22861 tgacgcttac gatgataacg agatagaaat tattcgttca aaagaattca gcttaaaacc
    22921 aatggattca gaagaagcgg tattacaaat gaatctatta ggtcatgatt tctttgtatt
    22981 cacagacaga gaaactgatg gaacaagtat cgtttaccgc cgtaaagacg gtaaatatgg
    23041 cttgattcaa actagtgaac aataaattaa gtttaaagca cttgtgtttt tgcacaagtg
    23101 ctttttttag acttcaaaag caatttctaa ctatttcaca gttcgtttgt gcaatttgtt
    23161 gaatgaaaca tagtgactat gctaatgtta atggatgtat atatttgaat gttaagttaa
    23221 taatagtatg tcagtctatt gtatagtcta agtcaaaaat cgtaaaatat ttataatata
    23281 atttattaaa aagtataatt gcgtattgag aatatattta ttagtgataa acttgttgtc
    23341 aacagaatgt gaatgaagta tgtcataaat atatttatat tgattctaca aatgagtaaa
    23401 taagtataat tttctaacta taaatgataa gatatattgt tgtaggccaa acagtttttt
    23461 agctaaagga gcgaacgaaa tgggattttt atcaaaaatt cttgatggca ataataaaga
    23521 aattaaacag ttaggtaaac ttgctgataa agtaatcgct ttagaagaaa aaacggcaat
    23581 tttaactgat gaagaaattc gtaataaaac gaaacaattc caaacagaat tagctgacat
    23641 tgataatgtc aaaaagcaaa atgattattt agataaaatt ttaccagaag catatgcact
    23701 tgttagagaa ggctctaaac gtgtattcaa tatgacacca tataaagttc aaattatggg
    23761 tggtattgca attcataaag gtgatatcgc tgagatgaga acaggtgaag gtaaaacgtt
    23821 aacagcgaca atgccaacat acttaaatgc attagctggt agaggtgttc acgttattac
    23881 agtcaatgaa tatttatcaa gtgttcaaag tgaagaaatg gctgagttat ataacttctt
    23941 aggtttgact gtcggattaa acttaaacag taagacgact gaagaaaaac gtgaagcata
    24001 tgcgcaagac attacttaca gtactaacaa tgagttaggt tttgactatt taagagataa
    24061 catggtgaat tattctgaag atagagtaat gcgtccatta catttcgcaa tcattgatga
    24121 ggttgactca attttaattg atgaggcacg tacgccatta attatttctg gtgaagctga
    24181 aaagtcaacg tcactttata cacaagcaaa tgtttttgcg aaaatgttaa agcaggacga
    24241 agattataaa tatgatgaaa aaacaaaagc agtacattta acagaacaag gtgcggataa
    24301 agctgaacgt atgttcaaag ttgaaaactt atatgatgta caaaatgttg atgttattag
    24361 tcatatcaac acagctttac gtgcgcacgt tacattgcaa cgtgacgtag actatatggt
    24421 tgttgatggc gaagtattaa ttgtcgacca atttacagga cgtacaatgc caggccgtcg
    24481 tttctcggaa ggtttacacc aagctattga agcgaaggaa ggcgttcaaa ttcaaaatga
    24541 atctaaaact atggcgtcta ttacattcca aaactatttc agaatgtaca ataaacttgc
    24601 gggtatgaca ggtacagcta aaactgaaga agaagaattt agaaatattt ataacatgac
    24661 agtaactcaa attccgacaa ataaacctgt gcaacgtaac gataagtctg atttaattta
    24721 cattagccaa aaaggtaaat ttgatgcagt agtagaagat gttgttgaaa aacacaaggc
    24781 agggcaacca gtgctattag gtactgttgc agttgagact tctgaatata tttcaaattt
    24841 actaaaaaaa cgtggtatcc gtcatgatgt gttaaatgcg aaaaatcatg aacgtgaagc
    24901 tgaaattgtt gcaggcgctg gacaaaaagg tgccgttact attgccacta acatggctgg
    24961 tcgtggtaca gatatcaaat taggtgaagg cgtagaggaa ttaggcggtt tagcagtaat
    25021 aggtacagaa cgacatgaat ctcgtcgtat tgatgaccag ttacgtggtc gttctggacg
    25081 tcaaggtgat aaaggggata gtcgcttcta tttatcatta caagatgaat taatgattcg
    25141 ttttggttct gaacgtttac agaaaatgat gagccgacta ggtttagatg actctacacc
    25201 aattgaatca aaaatggtat caagagctgt agaatcagca caaaaacgtg tagaaggtaa
    25261 taacttcgac gcgcgtaaac gtatcttaga atacgatgaa gtattacgta aacaacgtga
    25321 aattatctat aacgaaagaa atagtattat tgatgaagaa gacagctctc aagttgtaga
    25381 tgcaatgcta cgttcaacgt tacaacgtag tatcaattac tatattaata cagcagatga
    25441 cgagcctgaa tatcaaccat tcatcgacta catcaatgac attttcttac aagaaggtga
    25501 cattacagag gatgatatca aaggtaaaga tgctgaagat attttcgaag tcgtttgggc
    25561 taagattgaa gcagcatatc aaagtcaaaa agatatctta gaagaacaaa tgaatgagtt
    25621 tgagcgtatg attttacttc gttctattga tagccattgg actgatcata tcgacacaat
    25681 ggatcaatta cgtcaaggta ttcacttacg ttcttatgca cagcaaaatc cattacgtga
    25741 ctatcaaaat gaaggtcatg aattatttga tatcatgatg caaaatatcg aagaagatac
    25801 ttgtaaattc attttaaaat ctgtagtaca agttgaagac aatattgaac gtgaaaaaac
    25861 aacagagttt ggtgaagcga agcacgtttc agctgaagat ggtaaagaaa aagtgaaacc
    25921 gaaaccaatc gttaaaggcg atcaagttgg tcgtaacgat gattgtccat gtggtagtgg
    25981 taaaaaattc aaaaattgcc atggaaaata aatgatataa aataactcct tccaattaaa
    26041 cacctatagt ttgtgttatg ggaggagtct ttttatttta caagcgttaa atactttaaa
    26101 aaatgtgaag aagttgttaa acgttgttat gtacttagtt ttaaaaaata ggtttaggca
    26161 tatatcgatg ataaatgtac tgatttttaa caataaaagc ataaactgat tgtcagtgtg
    26221 cttatatttc ttaacattgt tatttaacaa aattatgtta aaatttagca ttataaatga
    26281 tacaaatcaa tgacttgaat tgaattataa ataggagcga aggctatgga attatcagaa
    26341 atcaaacgaa atatagataa gtataatcaa gatttaacac aaattagggg gtctctttga
    26401 cttagagaac aaagaaacta atattcaaga atatgaagaa atgatggcag aacctaattt
    26461 ttgggataac caaacgaaag cgcaagatat tatagataaa aataatgcgt taaaagcaat
    26521 agttaatggt tataaaacac tacaagcaga agtagatgac atggatgcta cttgggattt
    26581 attacaagaa gaatttgatg aagaaatgaa agaagactta gagcaagagg tcatttattt
    26641 taaggctaaa gtggatgaat acgaattgca attattatta gatgggcctc acgatgccaa
    26701 taatgcaatt ctagagttac atcctggtgc aggtggcacg gagtctcaag attgggctaa
    26761 tatgctattt agaatgtatc aacgttattg tgagaagaaa ggttttaaag ttgaaactgt
    26821 tgattattta cctggagatg aagcagggat taaaagtgta acattgctca tcaaagggca
    26881 taatgcttat ggttatttaa aagctgaaaa aggtgtacac cgactagtac gaatttctcc
    26941 atttgattca tcaggacgtc gtcatacatc atttgcatca tgtgacgtga ttccagattt
    27001 taataatgat gaaatagaga ttgaaatcaa tccggatgat attacagttg atacattcag
    27061 agcttctggt gcaggtggtc agcatattaa caaaactgaa tcggcaatac gaattaccca
    27121 ccacccctca ggtatagttg ttaataacca aaatgaacgt tctcaaatta aaaaccgtga
    27181 agcagctatg aaaatgttaa agtctaaatt atatcaatta aaattggaag agcaggcacg
    27241 tgaaatggct gaaattcgtg gcgaacaaaa agaaatcgga tggggaagcc aaattagatc
    27301 atatgttttc catccatact caatggtgaa agatcatcgt acgaacgaag aaacaggtaa
    27361 ggttgatgca gtgatggatg gagatattgg accatttatc gaatcatatt taagacagac
    27421 aatgtcacac gattaatata tattttaaaa ccgaggctct aaaagggcgt cggtttttgg
    27481 ttttttatag gtagctaaat aaattgtaaa ttagattttg gaatatgatt tgtttatgaa
    27541 tatttaagta caattgggta gatagggtta gaatatattt tttaaaagtt gtgtttgtta
    27601 aaacaacata tgcagtgtgc atttagtaat attacctatg gcgattttcg aggtattgaa
    27661 ttaatcgttg aaaacgttct caattacatg gtatgaatac attttacact atgataaaag
    27721 gttgtattct ttttatattg ttaaccattt gattacatcg ttataacaat agcttttgac
    27781 aaaatgtatt gtgctatagt atttgcatac ttaaaattct aacagcaaag gaatgacagc
    27841 aagatgaaaa aatctcttac agtgacggtt tcgtcagtgt tagctttttt agctttaaat
    27901 aatgcagcac atgcacaaca acatggcaca caagtaaaaa cacctgttca acataattat
    27961 gtatcaaatg ttcaagcaca aacgcaatca ccgacaactt atacagtagt tgctggcgat
    28021 tcattatata agattgcatt agagcatcac ttaacgttga atcaattata ttcatacaat
    28081 cctggtgtaa cacctttaat ttttcctggt gacgtgattt cacttgtgcc tcaaaataaa
    28141 gtgaaacaaa ctaaagcggt taaatcacca gtaagaaaag caagccaagc taaaaaggta
    28201 gtacaacaac ctgtacaaca agcgtctaaa aaagtagtag ttaagcaagc acctaagcaa
    28261 gcagtagcta agacagttaa tgtagcatac aaacctgctc aagtacaaaa atcagttcca
    28321 actgtacctg ttgcacataa ctacaataaa tcagttgcta acagaggaaa cttatatgct
    28381 tatggaaact gcacatatta tgctttcgat cgtcgtgcac aattaggtag aagtatagga
    28441 agtttatggg gcaatgcaaa taactggaat tacgcagcaa aagttgcagg atttaaagta
    28501 gataaaacac cagaagttgg cgctattttc caaacagctg ctggcccata tggacatgtt
    28561 ggtgttgttg aatctgtaaa ccctaatgga acaattactg tttctgaaat gaactatgct
    28621 ggatttaatg ttaaatcttc aagaacaatt ttaaatccag gaaaatataa ttacatccac
    28681 taagtaatat atcaagacaa gactatcctc ttagcctgtt taagtaacag gttgagagga
    28741 ttttttggta tcatttaatc agagttatat aaagaagata tttaaatgat atttaattga
    28801 ttgatatgta aaaagaaagt ataatttatg attaattaat ggaggaggta attgaaatgg
    28861 gtgtacatca atattttaaa agattatcag atatggaaag acttataaga ttacctggaa
    28921 aatttaaata ttttgaacac aatgttgcag cacactcctt taaagtaact aaaattgctc
    28981 aatatctagc aacagttgaa gaatatcatg gacgaaagat taattggaaa agcttatatg
    29041 aaaaagcatt aaatcatgat ttcgctgaag tgtttactgg tgatataaaa acacctgtta
    29101 aatatgcgag tagtgaatta aaaaaattat tttcgcaagt tgaagaagaa atggtagaga
    29161 cctttattga agaagaaatt ccattacaat atagagatgt ttataagcaa cgactgcaag
    29221 aaggtaaaga tgattcatta gaaggccaaa tactttcagt tgctgataaa attgatttgc
    29281 tttatgaaac atttggagaa atacaaaaac gtaatcctga agaattattt ttcgaaattt
    29341 atgaaatgag tctagaaaca attattcaat tcgaccattt agcatctgta caagatttta
    29401 ttaataatat cattccagaa atgttgactg aaaactttat acctagaaca gaattaagag
    29461 aaacaaccat gaacatttta aataaaagaa aagaggaaaa tgaatgatat ggtattttag
    29521 cgcagcattc tttccatgtg tcttggtagt attatttagt gtaataacaa gaagtaaatg
    29581 ggtcggtact attctgacat taattttaat tggtgcctca atctataaag agtatttcca
    29641 taacgagtgg attattttta ttgatgtagt gtcattatta gctggttatt taattataga
    29701 tcaactcgaa tttcataaac atcaagatga agatcgctaa gattaacttt aaaataatgt
    29761 ttcaaacaaa tttgttgaaa caaaatgatg attaatataa tgtgtattta catactaaaa
    29821 ataacaagat aaacgatttg atttaaggca agcatagtta gcacaactat gtttgttttt
    29881 ctttgttcga tatttttacg aacaaacgtt tgctttttgt gtgactactt tgctaaaata
    29941 tgtaatgaga aagcgaagag ttgagcggaa atataaataa tacgttgaaa gaggaggtat
    30001 atgtgacaat ggttgaacat tatcctttta aaatacattc tgattttgag cctcaaggtg
    30061 accaaccgca agcaattaag gaaatcgtgg aaggtattaa agcggggaaa agacatcaaa
    30121 ctttattagg tgcaactggc acagggaaaa catttacgat gagtaatgtt attaaagaag
    30181 ttgggaaacc aacgttaatt atcgcgcata acaaaacatt agcaggacaa ttatatagtg
    30241 agtttaaaga attttttcct gaaaacaggg tggaatactt tgtaagttac tatgattatt
    30301 atcaacctga ggcatacgta ccgtctactg acacttttat tgaaaaagat gcctcaatca
    30361 atgatgaaat tgatcaacta cgacattctg ctacaagtgc attatttgaa cgcgatgatg
    30421 taattattat tgctagtgta agttgtatat atggtttagg taatcctgaa gaatataaag
    30481 atttagtagt aagtgttcga gttggtatgg aaatggatag aagtgaatta cttagaaaac
    30541 ttgtagatgt gcaatataca cgaaatgaca tcgatttcca acgaggaacg tttcgagtgc
    30601 gtggtgatgt agttgaaata ttcccagcct ctaaagaaga actttgtata agggttgagt
    30661 ttttcggcga tgagattgac cgtatccgag aagttaacta cctaacaggt gaagtgttga
    30721 aagaaagaga acattttgcg atattcccag cttctcactt cgtaacacgt gaagaaaagt
    30781 tgaaagttgc gattgaacgt attgaaaaag aattggaaga acgattgaaa gaattacgaa
    30841 atgagaataa attactagaa gcgcaaaggt tagaacagcg taccaactat gatttagaaa
    30901 tgatgcgaga gatgggattc tgttcaggaa ttgaaaacta ttccgtacat ttaactttgc
    30961 gaccactggg ttcgacacca tatactttat tggattactt tggcgatgat tggttagtaa
    31021 tgattgatga atcacatgtg acattaccgc aagttcgagg catgtataac ggagacagag
    31081 cgcgtaaaca agttttggtg gatcatggat ttagattacc gagtgcatta gataaccgtc
    31141 ctcttaaatt tgaagaattt gaagaaaaga caaaacaact tgtgtatgta tctgcaacgc
    31201 ctggaccata cgaaattgaa catacggata agatggttga acaaattatt cgtcctactg
    31261 gtttactgga tcctaagatt gaggttagac ctactgaaaa tcaaattgac gatttattaa
    31321 gtgaaattca aacaagggtt gaacgtaatg aacgcgtact tgttacaacg ctcactaaaa
    31381 agatgagtga agatttaacc acatacatga aagaagcggg tattaaagtt aattatctgc
    31441 attcagaaat caagacatta gaacgaatcg aaataattag agacttacga atgggtacat
    31501 atgatgttat cgtaggtatt aatttattaa gagagggtat tgatatacca gaagtttctc
    31561 tagttgtcat attagatgca gataaagaag gatttttacg ttctaaccgc tctttaattc
    31621 aaacaatagg tagagctgcg cgtaacgata aaggtgaagt cattatgtat gccgataaaa
    31681 tgactgattc gatgaagtat gcaattgatg agacacaacg tcgtcgagaa atacagatga
    31741 aacataatga aaaacatggt attacaccta aaacaattaa taaaaaaata catgatttaa
    31801 ttagtgctac tgttgaaaat gacgaaaata atgacaaagc acaaactgtg atacctaaga
    31861 agatgacgaa aaaagaacgt caaaagacaa tcgacaatat agaaaaagaa atgaaacaag
    31921 cagcgaaaga tttagatttc gagaaagcta cagaattaag agatatgtta tttgaattaa
    31981 aagcagaagg gtgacaagta aatgaaagaa ccatccatag tagtaaaagg tgctcgtgcg
    32041 cataacttga aagatattga tatcgaacta cctaaaaata aattaattgt tatgacagga
    32101 ttatctgggt caggtaaatc gtcattagca ttcgatacta tatatgctga aggacaacga
    32161 cgttatgttg aatcattaag tgcctatgcg cgtcaatttt taggccaaat ggacaaacca
    32221 gatgttgata caatcgaagg attatcgcca gcaatttcaa tagatcaaaa aacaacaagt
    32281 aaaaatccaa gatcaactgt tgcaacagta acagaaatat atgattatat acgtttgtta
    32341 tatgcacgtg ttggtaaacc ttactgtcca aatcacaata tagaaattga atcacaaaca
    32401 gtacaacaaa tggttgaccg cattatggag ttagaggcac gtacaaagat tcaattatta
    32461 gcacctgtca tcgctcatcg taaaggtagt catgaaaagc taatcgaaga tattggtaaa
    32521 aaaggttatg tacgtttaag aatcgatggc gaaattgttg atgtaaatga tgtacctaca
    32581 ttagataaga acaagaatca tacaatagaa gttgttgtag accgattagt tgttaaagat
    32641 ggaattgaaa cacgactagc tgactctata gaaactgcgt tagagctttc agaaggacaa
    32701 ttaacagttg atgtcattga cggggaagac cttaagtttt cagaaagcca tgcttgtcct
    32761 atatgtggat tttcaatcgg agaattagaa ccaagaatgt ttagctttaa tagtcctttt
    32821 ggtgcttgtc caacatgtga tggcttaggc caaaagttaa cagtcgatgt agacttggtt
    32881 gttccagaca aagataagac gctaaacgaa ggtgcaatag aaccttggat accgacgagt
    32941 tctgattttt atccaacatt gttaaaacgt gtttgtgaag tttataaaat caatatggat
    33001 aaacctttta aaaagttaac agaacgtcaa cgtgatattt tattgtatgg ttcgggtgac
    33061 aaagaaattg aatttacatt tacacaacgt caaggtggta ctagaaaacg aacaatggtt
    33121 ttcgagggtg tagttcctaa tataagtaga cgattccatg aatctccttc agaatataca
    33181 cgtgaaatga tgagtaaata tatgactgaa ctaccttgtg aaacttgtca tggaaagcga
    33241 ttgagtcgtg aagcgttatc tgtttatgta ggtggtttaa atattggtga agtagtcgaa
    33301 tactcaatca gtcaagcgct gaactattat aaaaacattg atttgtcaga acaagatcaa
    33361 gcgattgcaa atcaaatatt gaaagaaatt atttcccgac tcactttttt aaataatgtc
    33421 ggacttgaat atttaacgct aaacagagct tcaggtacac tttcaggtgg tgaagcacaa
    33481 cgtattcgat tggcaacgca aattgggtcg cgtttgactg gtgtcttata tgtattagat
    33541 gagccatcaa ttggactgca tcaaagagat aatgatcgat taattaatac acttaaagaa
    33601 atgagagatt taggaaatac tttaattgta gttgaacacg atgatgatac aatgcgtgcg
    33661 gctgattact tagtggatat agggcctggt gctggtgaac atggaggaca aattgtgtct
    33721 agtgggacac ctcaaaaggt aatgaaagat aaaaaatctt taacaggaca atacttgagt
    33781 ggtaagaaac gtattgacgt acctgaatat cgcagaccgg cttcagatcg taaaatttct
    33841 atacgtggag ctagaagtaa caaccttaaa ggcgttgatg tggacatacc actatcaatc
    33901 atgacggtcg ttacaggtgt atcaggttct ggtaaaagct cattagtaaa tgaagtatta
    33961 tacaaatcat tagctcaaaa aatcaataaa tctaaagtaa agccaggatt gtacgataag
    34021 attgaaggta ttgatcaact tgataaaatt attgatattg atcaatcacc gataggtaga
    34081 acgccacgct ctaatccagc aacatatact ggtgtgtttg atgatatacg tgatgtgttt
    34141 gcgcaaacaa atgaagctaa aatccgagga tatcaaaaag ggcgttttag ttttaatgta
    34201 aaaggtggac gctgtgaagc ttgtaaaggt gacggtatta ttaaaattga aatgcatttt
    34261 ttacctgatg tttatgttcc ttgtgaagtg tgtgatggta aacgatataa tcgtgagaca
    34321 ctagaggtta cttacaaagg taaaaatatt gctgacattt tagaaatgac tgttgaagaa
    34381 gcaacacaat tttttgaaaa tattcctaag attaagcgca agttacaaac actagttgat
    34441 gttggtcttg gatacgtcac attaggtcaa caagctacaa cgttatcagg tggtgaggct
    34501 caacgtgtga aacttgcatc tgaacttcat aaacgttcaa ctggtaaatc tatttatatc
    34561 ctagacgaac caacaacagg gttacatgtt gacgatatta gcagattatt aaaagtatta
    34621 aaccgattag ttgaaaatgg tgatactgtt gtaattattg aacataacct agatgttatc
    34681 aaaacagcag actatattat cgacttaggt cctgaaggtg gtagtggcgg tggtactatt
    34741 gttgcgactg gcacacccga agatattgct cagacaaagt catcctatac aggaaagtat
    34801 ttaaaagaag tacttgaacg agataaacaa aatactgaag ataaataaga ttaaaagaag
    34861 tgaaggatgt tgtaaattta tccttcactt ctttttatta atttagtaat gaatagtaga
    34921 aagaaaagaa gcgtaaaaag aattatgtta agatagggtc aatctagagt tgctaaacat
    34981 aaatcgaact gggagtggga cagaaatgat attttcgcaa aatttatttc gtcgtcccac
    35041 cccaactagc attgtctgta gaaattggga atccaatttc tctgtgttgg ggccccgcca
    35101 actaattcca atatatcatt ttagagttta ggtcattgat ttatggcttg gacttttatg
    35161 tcgatatgaa ccatgtaaat taagcattca ataaattaat gattgatatt gacttgtaaa
    35221 ataataacaa taatgaacaa ttaatattta ttttagcttt tcaatgtaga ttggtgttat
    35281 atttttgata tgataagaag agatgtaaga gtagggataa atacaattga ggtgaaccca
    35341 tgttaacgac agaaaaacta gttgaaacat taaagttaga tttaatcgct ggtgaagaag
    35401 gactatcgaa gccaattaaa aatgctgata tatcaagacc gggcttagag atggcaggtt
    35461 atttttcaca ttatgcgtca gatagaatac aactattagg aacaacggaa ctatcgtttt
    35521 acaatttatt accagataag gatcgcgcag gtcgtatgcg taaactatgc agaccagaaa
    35581 cgcctgcaat tattgtgaca cgtggattgc agccaccaga agaattagtt gaagctgcaa
    35641 aagaattaaa tacgccactt atagttgcta aagatgcaac tacaagttta atgagtcgat
    35701 taacaacgtt tttagagcat gcacttgcaa agacgacatc tttacatggt gttttagtgg
    35761 atgtttacgg tgtaggcgta ttaattaccg gtgattctgg tattggtaaa agtgagactg
    35821 cgttagaatt agtgaagcgt gggcatagac tagtagcaga tgataatgta gaaatacgtc
    35881 aaattaataa agatgaacta atagggaaac caccaaagtt aatagaacat ctattagaaa
    35941 tacgtggact aggtattatc aatgttatga ctttatttgg cgcgggttca atattaactg
    36001 aaaaacgaat tagattaaat attaatttgg aaaactggaa caagcaaaag ttatatgacc
    36061 gcgtaggtct taatgaagag acgttaagta ttttagatac tgaaataact aaaaaaacaa
    36121 tacctgtaag acctggtaga aatgttgcgg taattattga ggtcgctgca atgaactatc
    36181 gattaaatat catgggcatt aacactgccg aagaatttag tgaaagatta aatgaagaaa
    36241 ttatcaagaa cagtcataag agtgaggagt aggttgaatg ggtattgtat ttaactatat
    36301 agatcctgtg gcatttaact taggaccact gagtgtacga tggtatggaa ttatcattgc
    36361 tgtcggaata ctacttggtt actttgttgc acaacgtgca ctagttaaag caggattaca
    36421 taaagatact ttagtagata ttatttttta tagtgcacta tttggattta tcgcggcacg
    36481 aatctatttc gtgattttcc aatggccata ttacgcggaa aatccaggtg aaattattaa
    36541 aatatggcat ggtggtatag caatacatgg tggtttaata ggtggcttta ttgctggtgt
    36601 tattgtatgt aaagtgaaaa atttaaaccc atttcaaatt ggtgatatcg ttgcgccaag
    36661 tataatttta gcgcaaggaa ttggacgctg gggtaacttt atgaatcacg aggcacatgg
    36721 tggacctgtg tcacgcgctt ttttagaaca attacatttg cctaatttta taatagaaaa
    36781 tatgtatatc aacggccaat attatcatcc aacattctta tatgaatcca tttgggatgt
    36841 cgctggattt attatcttag ttaatattcg taaacattta aaattaggag aaacattctt
    36901 tttatattta acttggtatt caattggtcg attctttata gaaggattac gtacagatag
    36961 cttaatgctc acaagtaata ttagagttgc acaattagta tcaattcttt taattttaat
    37021 aagtataagt ttaattgtat atagaaggat taagtataat ccaccgttgt atagcaaagt
    37081 tggggcgctt ccatggccaa caaaaaaagt gaagtagtga tattttgaga aaatttttat
    37141 caaaaacaca tcatcataca aaccctttat ggcgtgtata ccgtcttgtt aaattttcga
    37201 aagtttttaa gaatgtaatt atcattgaat tttcgaaatt tattccaagt atggtactga
    37261 aaagacatat atataaacaa cttttaaata ttaatatcgg taatcaatcg tcgatagctt
    37321 acaaagtaat gttagatatt ttttacccag aactgattac gattggtagt aacagtgtta
    37381 ttggttacaa tgtaacaatt ttgacgcatg aagcattagt tgatgaattt cgttatggac
    37441 cagtgacgat aggatctaac actttgattg gtgcaaatgc taccatttta cccggtataa
    37501 cgattggtga caatgtaaaa gttgcagctg gtacggttgt ttcaaaagat ataccggata
    37561 atggatttgc atatggcaac cctatgtata taaaaatgat taggaggtga caattttatg
    37621 gcgcaaaaga ataataacgt aattccaatg acttttgatg atgcatttta tcgtaaaatg
    37681 gctaatcaga agtttaaaca aagagaatat aaacgagctg ctgaatactt tgaaaaagtg
    37741 ttagaattgt cacctgatga tctggaaatt caaattgatt atgcacaatg tctagtgcaa
    37801 cttggtattg ctaaaaaagc agaacattta ttttatgaca atattattta taataggcat
    37861 ctagaagata gcttttatga attgagtcag ctcaacattg aagttaacga accaaacaag
    37921 gcattcttgt ttggtattaa ttatgttatt gttagcgacg accaagatta tagagatgaa
    37981 ttagatcaaa tgtttgatgt gaaatatcaa agtgaagaac aaattgaact tgaagctcaa
    38041 ttgtttgtag ttcaaatact attccaatat cttttttctc aaggtcgatt aaaagatgca
    38101 aagaattatg tcttacatca accacaagaa gttcaagatc atcgtgtcgt acgtaattta
    38161 ttggcaatgt gttatttata tctcggcgaa tatgacacgg ctaaagcatt gtacgaagca
    38221 ctattacaag aggatagtac agatatatat gcattatgcc attatacttt gctactttat
    38281 aacactaagg aaaatgaaca ataccaaaaa tatttaaaaa tattaaataa agttgtacct
    38341 atgaatgacg atgaaagttt taaattaggt attgttttaa gttatttaaa gcagtatcgt
    38401 gcatcacaac aattgttgta ccctttatat aaaaaaggga aatttttatc aattcaaatg
    38461 tacaatgctt tagcatataa ttattattat ttaggtgaag aagacgaaag tcattactac
    38521 tgggataaat tgaagcaaat ttctaaagtg gaaattggac atgcgccttg ggtaattgaa
    38581 aatagcaaag aagttttcga ccaacatatt ttgccattac ttcaaagtga tgacagtcat
    38641 tatcgtttat atggtatttt tttattggat caattaaatg gtaaagaaat tgtgatgacg
    38701 gaaagtattt ggcaggttct ggaaaatcta aataattatg agaaattgta tttaacgtat
    38761 ttagttcaag gtttaacgct caataaatta gacttcattc atcgcggctt gttaacgctt
    38821 taccataatg aattatttgt gagtgaaaat gatgtaatgg ttgcatggat taatcaaggt
    38881 gaactcataa ttgctgaaaa agtagattta actgatgttg agccatatat cggtgcgttt
    38941 atttatttgt attttaaaaa tcaacctcga aatgttacaa agaaccaaat tacaacatgg
    39001 ttaggcataa cacaatataa actgaacaaa atgattgaat ttctcttgag catatagatt
    39061 tatgaaaagt tagatttatt atataatgcg cataatgatt aataatgagg aggcgttaat
    39121 aaaatgactg aaatagattt tgatatagca attatcggtg caggtccagc tggtatgact
    39181 gctgcagtat acgcatcacg tgctaattta aaaacagtta tgattgaaag aggtattcca
    39241 ggcggtcaaa tggctaatac agaagaagta gagaacttcc ctggtttcga aatgattaca
    39301 ggtccagatt tatctacaaa aatgtttgaa cacgctaaaa agtttggtgc agtttatcaa
    39361 tatggagata ttaaatctgt agaagataaa ggcgaatata aagtgattaa ctttggtaac
    39421 aaagaattaa cagctaaagc ggtcattatt gctacaggtg cagaatacaa gaaaattggt
    39481 gttccgggtg aacaagaact tggtggacgc ggtgtaagtt attgtgcagt atgtgatggt
    39541 gcattcttta aaaataaacg cctattcgtt atcggtggtg gtgactcagc agtagaagag
    39601 ggaacattct taactaaatt tgctgacaaa gtaacaattg ttcaccgtcg tgatgagtta
    39661 cgtgcacaac gtattttaca agatagagca ttcaaaaatg ataaaatcga ctttatttgg
    39721 agtcatactt tgaaatcaat taatgaaaaa gacggcaaag tgggttctgt gacattaacg
    39781 tctacaaaag atggttcaga agaaacacac gaggctgatg gtgtattcat ctatattggt
    39841 atgaaaccat taacagcgcc atttaaagac ttaggtatta caaatgatgt tggttatatt
    39901 gtaacaaaag atgatatgac aacatcagta ccaggtattt ttgcagcagg agatgttcgc
    39961 gacaaaggtt tacgccaaat tgtcactgct accggcgatg gtagtattgc agcgcaaagt
    40021 gcagcggaat atattgaaca tttaaacgat caagcttaat tcgaagtcga attaagatgt
    40081 tgagttgtaa attatttaga tatttatttt aatagtgtca tcacattgtt aaaataatgt
    40141 cttactttta aattaaagca aattatatag aaaactagaa cttagtacgt atcatttgtg
    40201 cgtttcaatg agttctagtt tttttatatg ttatattaaa cttataactt tatgggagta
    40261 ggacagaaat gataaagagc cactaatgat ttattatgta gtggttctta cacattagc
//
LOCUS       DAHHOJ010000011        39871 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.011, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000011 DAHHOJ010000000
VERSION     DAHHOJ010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 39871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.011"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..225)
                     /locus_tag="NBH32_000470"
     CDS             complement(<1..225)
                     /locus_tag="NBH32_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin MazF"
                     /protein_id="HCD1514102.1"
                     /translation="MTHNIEKRINKLKTSGNPKFKKLDSDIHYLLKRFEGEKNHKGFY
                     PKFKQGEIVFVDFGINVNKEFSNSHFAIVMN"
     gene            complement(303..485)
                     /locus_tag="NBH32_000471"
     CDS             complement(303..485)
                     /locus_tag="NBH32_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000694772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514103.1"
                     /translation="MKITNCKIKKETIVYEVLTSGNQPFTYELPKDLSSHNACKYLEF
                     ISQKIDGDKLTKEDSL"
     gene            complement(556..741)
                     /locus_tag="NBH32_000472"
     CDS             complement(556..741)
                     /locus_tag="NBH32_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514104.1"
                     /translation="MSTYKEIEHLHINTGGKELTQEQIEEAKAFIDSQEFKDMIREAK
                     ESHQRVMESKITDRTKL"
     gene            complement(738..884)
                     /locus_tag="NBH32_000473"
     CDS             complement(738..884)
                     /locus_tag="NBH32_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000345949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514105.1"
                     /translation="MDFKEVDINIEEWEMVEIPFYTEEELTYRLNNGLPITKSELEEQ
                     ESKK"
     gene            complement(958..1812)
                     /locus_tag="NBH32_000474"
     CDS             complement(958..1812)
                     /locus_tag="NBH32_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIRAN domain-containing protein"
                     /protein_id="HCD1514106.1"
                     /translation="MKPRKQDEKILSDQYSYFEPIISDSCDIKFDENKRRMGSIFISH
                     EEICFIRKEEDYIFKISLSEVIDYNTVVTIWKNQAFLTLNDNRKLTVYFVTNSPLTGF
                     ISILKTYMQLSKNKETIISNDCLPINDDEQTKVEIFDVVGLNYEGRRKELKKLIKKMK
                     NNDDFFFLYSDLKGNELKEELLYEDKVYEISDYEVIPGVFLQKEPDNPYDENAIKVMI
                     SNEYSEFHVGYVPREYASRLVNHMDNIVSCNAYINGGKYKTLDYLEEKIVTKESDYGL
                     RVHLEYKV"
     gene            complement(1824..2540)
                     /locus_tag="NBH32_000475"
     CDS             complement(1824..2540)
                     /locus_tag="NBH32_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001083977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1514107.1"
                     /translation="MNSFKDRLKQIMSERKISQSELSRRTGIGRNSISDYLNGKYEAK
                     QDKVFELAKALNVNEAWLMGFDISKNRKIENNDITSIYSKLTPPRQSNVLKYATNQLE
                     EQNNDSDNLVDFNSYIQEKSEVDIYGCASAGIGERLYNEPISKEFVRGYVPAHDIALK
                     VNGDSMEPLFKNGQIIFIEKSHTIKDGQIGVFIINGDAYVKKVYVEDNRLTLVSLNKK
                     YKDLYFYDNESVRLVGKVIL"
     gene            2704..2946
                     /locus_tag="NBH32_000476"
     CDS             2704..2946
                     /locus_tag="NBH32_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000639923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF739 family protein"
                     /protein_id="HCD1514108.1"
                     /translation="MIYNFDYSLLYERMAEYRYSQSSLANAIPISRTSINHKLQGKNL
                     FTQWEIKRICELLEIPPTKVGRYFFEQNVQKPVQMS"
     gene            2959..3405
                     /locus_tag="NBH32_000477"
     CDS             2959..3405
                     /locus_tag="NBH32_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514109.1"
                     /translation="MEQITLTKEELKEIIAKEVREAINGKKPISSGSIFNKVRISHND
                     FDEINKKFAYTERLRGADNLGLGHPLSLKKYQHGIGCYENYKAYASEIHDHIRKLTLS
                     AFGVTLNSDLKESEYDEASRMYDMLKNFYLYRYQKRIETLSIEDFE"
     gene            3420..3560
                     /locus_tag="NBH32_000478"
     CDS             3420..3560
                     /locus_tag="NBH32_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514110.1"
                     /translation="MLQKFRIAKEKNKLKLKLLKHASYCLERNNNPELLRAVAELLKK
                     VS"
     gene            complement(3553..3762)
                     /locus_tag="NBH32_000479"
     CDS             complement(3553..3762)
                     /locus_tag="NBH32_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514111.1"
                     /translation="MKLLVTLKDGSKKHVSDLKKIVFPGYEGIETVTKEEIETFFLDP
                     TKTYVFVGSQTLSVEAGQILTVEFS"
     gene            3819..4568
                     /locus_tag="NBH32_000480"
     CDS             3819..4568
                     /locus_tag="NBH32_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001148641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor KilAC domain-containing
                     protein"
                     /protein_id="HCD1514112.1"
                     /translation="MQALQTKSNIGEMFNIQEKENGEIAISARELYKALEVKKRFSAW
                     AEINLKHFKENRDFTSVLTSTVVNNGAVRQLEDYALTLDVAKHVAMMSGTEKGFDFRE
                     YFIQVEKAWNSPEMIMQRALKIANNTINQLETKIERDKPKIVFADAVATTKTSILVGE
                     LAKIIKQNGVNIGQRRLFEWLRQNGFLIKRKGVDYNMPTQYSMERELFEIKETSITHS
                     DGHTSISKTPKVTGKGQQYFVNKFLGEKQTS"
     gene            4581..4841
                     /locus_tag="NBH32_000481"
     CDS             4581..4841
                     /locus_tag="NBH32_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1514113.1"
                     /translation="MEQITLTKEECVEQCINKDLKLLDYRVQQILEGVLSESTTYGDA
                     RNKLETLKIIAESHFKTEHASVIYKLALKKLDKKINATPIKE"
     gene            4861..4998
                     /locus_tag="NBH32_000482"
     CDS             4861..4998
                     /locus_tag="NBH32_000482"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514114.1"
                     /translation="MFKILNDIKTSLKNHPWGWKEHLPYLLMLTLSLVALIFGVLSAI
                     L"
     gene            5074..5394
                     /locus_tag="NBH32_000483"
     CDS             5074..5394
                     /locus_tag="NBH32_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HCD1514115.1"
                     /translation="MPHVLNVTVPIPETHVLITKDEYEELIAYSLDPVWNMSDLKKKL
                     KIASDETIKDRLLFHPRFEKELRAQGIVHYPDENFNRWRFNARKMNKFVDEHFNEIYK
                     ERIK"
     gene            5391..5552
                     /locus_tag="NBH32_000484"
     CDS             5391..5552
                     /locus_tag="NBH32_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1270 domain-containing protein"
                     /protein_id="HCD1514116.1"
                     /translation="MSNIYKSYLLAVLCFTVLAIVLMPFLYFTTAWSIAGFASIATFI
                     FYKEYFYEE"
     gene            5645..5905
                     /locus_tag="NBH32_000485"
     CDS             5645..5905
                     /locus_tag="NBH32_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1108 family protein"
                     /protein_id="HCD1514117.1"
                     /translation="MYYKIGDVCQKVINVDGFDFKLAVKKQDYSILVNVLDLEDRFID
                     GINITDENDLYTALDILNQSIYEWIEENTDERDRLINLVMRW"
     gene            5914..6177
                     /locus_tag="NBH32_000486"
     CDS             5914..6177
                     /locus_tag="NBH32_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001638663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514118.1"
                     /translation="MRDTERNILNIFKTLFDEYTLSNQRALLEIERNHHGYLSINFLH
                     YHDSYKTNNKLVQIHEINPDSHERIKNLIIEVLRGHRKIKKGA"
     gene            6186..8129
                     /locus_tag="NBH32_000487"
     CDS             6186..8129
                     /locus_tag="NBH32_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000700558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1514119.1"
                     /translation="MKINKLTISNFAGIKEESFNFNGKDAKIYGNNATGKTTTATALQ
                     WLLFDKGLDGSTKSFNPVSLNEKNEENYELIPTVFAEFEIDGKITTFKKESHPKYTIN
                     QKTNRKEYSRSRTKKQYINDESIKVKDYKARIDELIDEDVFKLITNPQAFNLLDWKKR
                     RSLLFEIAKPINDEDVIKTNDDFKELNNILGDHEIETKKKILTDKIKQINKDIKDIPI
                     RINQTQQNKQDVPEFDNDRYAIIKQEIEQLENERIDIQNGKEEINLRNQLADKQSELK
                     RIEDNNSASNENKIHALTNELHVENGTVANLKTRLKQNKQQITHEENRRNQLLENHKG
                     LKSDLEKAKNQKFEYLDDNVCSCCGQQLPAEQVNEAREKALQKFNAGKSKELETIQTS
                     INHIISEGKKIKPIIEKLEDDNNNLQIKINEAEERSARIQNKINKLKTTHVDVTQTDE
                     YKAVMLEINEINQKRSNIRKTIQDNVSGIDDKISELTQEKSEIEVSRSIEKSNKHLDD
                     VISELRNEEDRLLDEKEKYSHDLYILKEFTTTKVKMLTENINNEFEIAEFKLFNTLVN
                     GELEETCSTTVNGVEYDSGLNNASRINVGLDIINTLSKHFKVTAPIFIDNAESVTELI
                     KTESQQIQLIVNEQDKKLRMETI"
     gene            8131..9051
                     /locus_tag="NBH32_000488"
     CDS             8131..9051
                     /locus_tag="NBH32_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000138475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HCD1514120.1"
                     /translation="MTENNKLQTIEQQLVQEKNVSDNVLNKVRVLESQGNLELPNDYS
                     PSNAMKQAWLQISQDNKLMSCNDTSKANALLDMVTQGLNPAKNQCYFIPYGNKMQLQR
                     SYHGNVMMLKRDAGAQDVVAQVIYKGDTFKQEMGGTGRIKAIKHEQDFFNIDKENIIG
                     AYCTIVFNDGRDNYIEVMTIEQIKQAWMQSSMIKDEKALQNSKTHNNFKEEMAKKTVI
                     NRAAKRYINTSTDSNLFKYAQESEQRQRKEVLDAEVEENANQEQLDFEQPVLEEAQYT
                     ELENDKPIDVSDFEEIKEPATEKESEEEPF"
     gene            9180..9749
                     /locus_tag="NBH32_000489"
     CDS             9180..9749
                     /locus_tag="NBH32_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000123904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1514121.1"
                     /translation="MAGCLITHEHGDHAKYTKQFVDNGVISYMTAGTQQAMNFESHRL
                     CTIKAKQELRIGTWSILPFDIEHDANEPVAFLLQSTLGYKVLYVTDTKYLKYKFNGIT
                     HMMLEVNYIYEQMQENIKNGSVHSTLANRIMESHFSLEHAIGMLKANDLTRLEEIHLI
                     HLSSQNSNAKYIKSEIQKVTGVPVYVGGL"
     gene            9750..10220
                     /gene="ssb"
                     /locus_tag="NBH32_000490"
     CDS             9750..10220
                     /gene="ssb"
                     /locus_tag="NBH32_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1514122.1"
                     /translation="MINRTILVGRLTRDPELRTTQSGVNVASFTLAVNRTFTNAQGER
                     EADFINIIVFKKQAENVNKYLSKGSLAGVDGRLQTRNYENKEGQRVYVTEVIADSIQF
                     LEPKNSNDTQQDLYQQQVQQTRGQSQYSNNKPVKDNPFANANGPIEIDDNDLPF"
     gene            10250..11134
                     /locus_tag="NBH32_000491"
     CDS             10250..11134
                     /locus_tag="NBH32_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HCD1514123.1"
                     /translation="MTGWIKLHRKLLDSPIFQNEKLFKVFAYCLMKASHKDHTQLVGR
                     RVVELEKGQFVFGRKRASEELRLKESTVRDYIKLLENLGTIVVKSDNKFSVITVVNWA
                     IYQSMEENSDSKNDNKSTTNGQQINNKSTTNGQQINTNKNVKNGDNVKNDENEKKKAA
                     AFDFFQDNGFGFITPYNLDDLNYYLDSFENDSDQIVTASLKIAKDRNKVTWGYAKSIL
                     NTWLNANLKSIEQIRAFEKQQLESKKQTNKPYVKPSKEKTPKWLTDSTRETKTPEVDE
                     NLEKDREAFIKRLNSKWE"
     gene            11141..11359
                     /locus_tag="NBH32_000492"
     CDS             11141..11359
                     /locus_tag="NBH32_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000338527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514124.1"
                     /translation="MDAFDKYYLFDHDGNKMFSVTPHFKDGRHLVVGIKETKFNGRRW
                     YLDDYELNTLIDNEQMELGHQTSLFEYI"
     gene            11368..11772
                     /locus_tag="NBH32_000493"
     CDS             11368..11772
                     /locus_tag="NBH32_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000401967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCD1514125.1"
                     /translation="MEIEIKFNEVFNAPMGSPRPRFRKTGRFVQTYMPTSYTKHKAYI
                     QGQMPKLNLERALKIELDFYFPLLKSWSKKKKSEMVGQYKVTNPDIDNLIKTVLDACN
                     GHVWKDDNQITEITSSKRYGIEPKIIIRIEEI"
     gene            11785..12153
                     /locus_tag="NBH32_000494"
     CDS             11785..12153
                     /locus_tag="NBH32_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514126.1"
                     /translation="MARKARIVTINDKPYRFSKFEMELIESHGITAGMVSKRVKDGWE
                     LHEAMDAPEGTRLSEYREKKTIERLEQARLERKLERKQKKEAELRRKKPHLFNVPQKH
                     PRGRYACYLMENDIFVKVKK"
     gene            12157..12399
                     /locus_tag="NBH32_000495"
     CDS             12157..12399
                     /locus_tag="NBH32_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phi PVL orf 51-like protein"
                     /protein_id="HCD1514127.1"
                     /translation="MTDNARKEYLNQFFGSKRYLYQDNERVAHIHVVNGTYYFHGHIV
                     PGWQGVKKMFDTAEELETYIKQHGLEYEEQKQLTLF"
     gene            12413..12799
                     /locus_tag="NBH32_000496"
     CDS             12413..12799
                     /locus_tag="NBH32_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000693615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514128.1"
                     /translation="MKIKFKKEMTLDELIKWAWENPELVRGEKFYAQGKSNETYVYFH
                     LYDGRKCILREYISADDTFEVEYEEEITEETVIPKLVKMYKDGKMSVYNDYSIKRSLL
                     YSPKAYYILNDDLTMTLIWKDGELVE"
     gene            12987..13436
                     /locus_tag="NBH32_000497"
     CDS             12987..13436
                     /locus_tag="NBH32_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000982712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514129.1"
                     /translation="MMPKYRVWDEYTGRIHDVVGFDFIETEVHYENYAEAEALIHARD
                     FKDVELMQSTGLKDKNNNEIYAGDIVEFEDEILEMPDDESVIGTINRAVISIDVVNGI
                     QLKDFMFEGAVSENDYFEYIDIKSFLRYDCEVKGNIFESSHLLEVTE"
     gene            13433..13717
                     /locus_tag="NBH32_000498"
     CDS             13433..13717
                     /locus_tag="NBH32_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514130.1"
                     /translation="MNYETGVQLGVMDARLKKMRKQRDEYKKQRDELIGDIGKLRERN
                     KELEKKASAWDRYCKSVEKDLINEFGKDGERVKFGMELNNKTFMEEDTNE"
     gene            13710..13964
                     /locus_tag="NBH32_000499"
     CDS             13710..13964
                     /locus_tag="NBH32_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1024 family protein"
                     /protein_id="HCD1514131.1"
                     /translation="MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILE
                     GMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQEDEKI"
     gene            13951..14121
                     /locus_tag="NBH32_000500"
     CDS             13951..14121
                     /locus_tag="NBH32_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015978317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514132.1"
                     /translation="MKKFNVQITYTGMIEETIEAESLDEAEFEAHDIARMEVPFDCDE
                     FEINVEVEQEND"
     gene            14114..14647
                     /locus_tag="NBH32_000501"
     CDS             14114..14647
                     /locus_tag="NBH32_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP pyrophosphatase"
                     /protein_id="HCD1514133.1"
                     /translation="MTNTLQVKLLSENARMPERNHKTDAGYDIFSAETVVLEPQEKAV
                     IKTDVAVSIPEGYVGLLTSRSGVSSKTHLVIETGKIDAGYHGNLGINIKNDNETLESE
                     YISNFGRSPSGIDGQYALLPVTDKILCMNGSYVINKGDKLAQLVIVPIWTPELKQVEE
                     FESVSERGAKGFGSSGV"
     gene            14684..14971
                     /locus_tag="NBH32_000502"
     CDS             14684..14971
                     /locus_tag="NBH32_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1381 domain-containing protein"
                     /protein_id="HCD1514134.1"
                     /translation="MTQYLVTTFKDSTGRKHTHITKAKSNQRFTVVEAESKEEAKEKY
                     KARNAPVDGATNLNDIKSNIGIFHVEKVEPNEGMVDINIETMKPFEEADDD"
     gene            14964..15200
                     /locus_tag="NBH32_000503"
     CDS             14964..15200
                     /locus_tag="NBH32_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000608273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514135.1"
                     /translation="MINIPKMKFPKKYTEIIKKYKNKTPEEKAKIEDDFIKEINDKDS
                     EFYSPMTANMNEHELRAMLRMMPSLIDTGDDNDD"
     gene            15190..15579
                     /locus_tag="NBH32_000504"
     CDS             15190..15579
                     /locus_tag="NBH32_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514136.1"
                     /translation="MMIKKLKNMDWFDIFIVGILRLFGVIALMLVVISPIYTVASYQN
                     KEVHQGTITDKYNKRQDKEDKFYIVLDNKQVIENSDLLLKKKFDSADIQAKLKIGDKV
                     EVKTIGYRIHFLNLYPVLYEVKKVDKK"
     gene            15576..15725
                     /locus_tag="NBH32_000505"
     CDS             15576..15725
                     /locus_tag="NBH32_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1514137.1"
                     /translation="MIKQILRLLFLLAMYELGKYVTEQVYIMMTANDDVEAPSDYVFR
                     AEVSE"
     gene            15725..15925
                     /locus_tag="NBH32_000506"
     CDS             15725..15925
                     /locus_tag="NBH32_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001557462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1514 family protein"
                     /protein_id="HCD1514138.1"
                     /translation="MWITMTIVFAILLLVCISINSDRAREIQALRYMNDYLLDEVVKT
                     KGYNGLKEYRIELKRMNNDIKK"
     gene            15953..16369
                     /locus_tag="NBH32_000507"
     CDS             15953..16369
                     /locus_tag="NBH32_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1514139.1"
                     /translation="MIKIEKHDIKKLEEYIQHIDNYRRELKMREYELLESHEPDNAGA
                     GKSNLPGNPIERCAIKKFSDNRYNTLRNIVNGVDRLIDESDEDTLELLRFRYWDCPIG
                     CYEWEDIAHYFGTSKTSILRRRNALIDKLAKYIGYV"
     gene            16601..16900
                     /locus_tag="NBH32_000508"
     CDS             16601..16900
                     /locus_tag="NBH32_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000988331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCD1514140.1"
                     /translation="MMTKDERIRFYKSKEWQTTRKRVLERDNYECQQCKRDGKLTTYD
                     KSKHKSLDVDHILSLQHHPEFAHDLNNLETLCIKCHNKKEKRFIKKENKWKDEKW"
     gene            17031..17375
                     /locus_tag="NBH32_000509"
     CDS             17031..17375
                     /locus_tag="NBH32_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514141.1"
                     /translation="MEIIVDENLVLKEKERLQVLYKDIPSNKLKVVDGLIIQAARLRV
                     MLDYMWEDIKEKGDYDLFTQSEKAPPYERERPVAKLFNARDAAYQKIIKQLSDLLPEE
                     KEDTETPSDDYL"
     gene            17372..19033
                     /locus_tag="NBH32_000510"
     CDS             17372..19033
                     /locus_tag="NBH32_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016187779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCD1514142.1"
                     /translation="MISNKYVDEYINLWKQGKIILNKERIDLFNYLQKHIYSRDDVYF
                     DEQKIEDCIKFIEKWYFPTLPFQRFIIANIFLIDKNTDEAFFTEFAIFMGRGGGKNGL
                     ISAISDFLSTPLHGVKEYHISIIANSEDQAKTSFDEIRTVLMDNKRNKTGKTPKAPYE
                     VSKAKIINRATKSVIRYNTSNTKTKDGGREGCVIFDEIHYFFGPEMVNVKRGGLGKKK
                     NRRTFYISTDGFVREGYIDAMKHKIASVLSGKVKNSRLFAFYCKLDDPKEVDDRQTWE
                     KANPMLHKPLSEYAKTLLSTIEEEYNDLPFNRSNKPEFMTKRMNLPEVDLEKVIAPWK
                     EILATNREIPNLDNQMCIGGLDFANIRDFASVGLLFRKNDDYIWLGHSFVRQGFLDDV
                     KLEPPIKEWEKMGLLTIVDDDVIEIEYIVDWFLKAREKYGLEKVIADNYRTDIVRRAF
                     EDAGIKLEVLRNPKAIHGLLAPRIDTMFAKHNVIYGDNPLMRWFTNNVAVKIKPDGNK
                     EYIKKDEVRRKTDGFMAFVHALYRADDIVDKDMSKALDALMSIDF"
     gene            19049..20212
                     /locus_tag="NBH32_000511"
     CDS             19049..20212
                     /locus_tag="NBH32_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000025279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCD1514143.1"
                     /translation="MSILEKIFKTRKDISYMLDLDMIEDLSQQAYVKRLAIDSCIEFV
                     ARAVAQSHFKVLEGNRIQKNDVYYKLNIKPNTDLSSDSFWQQVIYKLIYDNEVLIVVS
                     DSKELLIADSFYREEYALYDDIFKDVTVKDYTYQRTFTMQEVIYLKYNNNKVTHFVES
                     LFEDYGKIFGRMIGAQLKNYQIRGILKSASSAYDEKNIEKLQAFTNKLFNTFNKNQLA
                     IAPLIEGFDYEELSNGGKNSNMPFSELSELMRDAIKNVALMIGIPPGLIYGETADLEK
                     NTLVFEKFCLTPLLKKIQNELNAKLITQSMYLKDTRIEIVGVNKKDPLQYAEAIDKLV
                     SSGSFTRNEVRIMLGEEPSDNPELDEYLVTKNYEKANENGSTLKGGDEDESGD"
     gene            20196..20933
                     /locus_tag="NBH32_000512"
     CDS             20196..20933
                     /locus_tag="NBH32_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001607394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HCD1514144.1"
                     /translation="MKVEIKGVIVSNEDKWVYEMLGMDSTCPKDVLTQLEFSDEDVDI
                     IINSNGGNLVAGSEIYTHLRAHKGKVNVRITAIAASAASLIAMAGDHIEMSPVARMMI
                     HNPSSIAQGEAKDLNHAAETLEHVGQIMAEAYAVRAGKNKQELIEMMAKETWLNADEA
                     IEQGFADSKMFENDNMQIVASDTQVLSKDVLNRVTALVSKTPEVNIDIDAIANKVIEK
                     INMKEKESEIDVADSKLSANGFSRFLF"
     gene            20956..22101
                     /locus_tag="NBH32_000513"
     CDS             20956..22101
                     /locus_tag="NBH32_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001573791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HCD1514145.1"
                     /translation="MTINLSETFANAKNEFINAVNNGEPQERQNELYGDMINQLFEET
                     KLQAKAEAERVSSLPKSAQTLSANQRNFFMDINKSVGYKEEKLLPEETIDRIFEDLTT
                     NHPLLADLGIKNAGLRLKFLKSETSGVAVWGKIYGEIKGQLDAAFSEETAIQNKLTAF
                     VVLPKDLNDFGPAWIERFVRVQIEEAFAVALETAFLKGTGKDQPIGLNRQVQKGVSVT
                     DGAYPEKEEQGTLTFANPRATVNELTQVFKYHSTNEKGKSVAVKGNVTMVVNPSDAFE
                     VQAQYTHLNANGVYVTALPFNLNVIESTVQEAGKVLTYVKGLYDGYLAGGINVQKFKE
                     TLALDDMDLYTAKQFAYGKAKDNKVAAVWKLDLKGHKPALEDTEETL"
     gene            22121..22405
                     /locus_tag="NBH32_000514"
     CDS             22121..22405
                     /locus_tag="NBH32_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016170801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514146.1"
                     /translation="MVKFKVVREFKDIEHNQHKYKVGELYPAEGYNNPRVELLTNQIK
                     NKYDKVYIVPLDKLTKQELLELCESLQKKASSSMVKSEIIDLLNGEDNDD"
     gene            22395..22679
                     /locus_tag="NBH32_000515"
     CDS             22395..22679
                     /locus_tag="NBH32_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009560502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HCD1514147.1"
                     /translation="MTIDDLLVKFKSLEKIDHNSEDEYLKQLLKMSYERIKNQCGVFE
                     LENLIGQELILIRARYAYQDLLEHFNDNYRPEIIDFSLSLMEVSEDEESV"
     gene            22663..23025
                     /locus_tag="NBH32_000516"
     CDS             22663..23025
                     /locus_tag="NBH32_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000755150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head-tail adaptor protein"
                     /protein_id="HCD1514148.1"
                     /translation="MKKVFKKPRITTKRLNTRVHFYKYTENNGPEAGEKEEKLLYSCW
                     ASIDGVWLRELEQAISNGTQNDIKLYIRDPQGDYLPSEEHYLEIESRYFKNRLNIKQV
                     SPDLDNKDFIMIRGGYSS"
     gene            23022..23426
                     /locus_tag="NBH32_000517"
     CDS             23022..23426
                     /locus_tag="NBH32_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000114224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HCD1514149.1"
                     /translation="MSVKVTGDKALERELEKHFGIKEMVKVQDKALIAGAKVIVEEIK
                     KQLKPSEDSGALISEIGRTEPEWIKGKRTVTIRWRGPFERFRIVHLIENGHVEKKSGK
                     FVKPKAMGGINRAIRQGQNKYFETLKRELKKL"
     gene            23423..23830
                     /locus_tag="NBH32_000518"
     CDS             23423..23830
                     /locus_tag="NBH32_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000565496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514150.1"
                     /translation="MIDILYKVHEVISQDRIIREHVNINNIKFNKYPNVKDTDVPFIV
                     IDDIDDPIPTTYTDGDECAYSYIVQIDVFVKYNDEYNARIIRNKISNRIQKLLWSELK
                     MGNVSNGKPEYIEEFKTYRSSRVYEGIFYKEEN"
     gene            23831..24472
                     /locus_tag="NBH32_000519"
     CDS             23831..24472
                     /locus_tag="NBH32_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000268736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCD1514151.1"
                     /translation="MAIKHASAPKAYFNITGLGFAKLTKEGAELKYSDITKTRGLQKI
                     GVETGGELKTAYADGGPIESGNTDGEGKISLQMHAFPKEIRKIVFNEDYDEDGVYEEK
                     QGKQNNYVAVWFRQERRDGTFRTVLLPKVMFTNPKIDGETAEKDWDFSSEEVEGEALF
                     PLIDNKKSVRKYIFDSANMTNHGGDGEKGEEAFLKKILGEEYTGNVTEDTETL"
     gene            24433..24738
                     /locus_tag="NBH32_000520"
     CDS             24433..24738
                     /locus_tag="NBH32_000520"
                     /inference="COORDINATES: protein motif:HMM:NF014427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCD1514152.1"
                     /translation="MYWKRDRGYGNFVTKPASSETAVKSVSIPDSIKTLKVGNTYDLN
                     VVVEPSNQRKLVKYTTDQTNVVSINSDGQVTAEAQGIATVKATVGNMSDTITINVEA"
     gene            24789..25139
                     /locus_tag="NBH32_000521"
     CDS             24789..25139
                     /locus_tag="NBH32_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514153.1"
                     /translation="MAKLKRNIIQLVEDPKANEIKLQTYLTPHFISFEIVYEAMDLID
                     DIEDENSTMKPREIADRLMDMVVKIYDNQFTVKDLKERMHAPDGMNALREQVIFITQG
                     QQTEETRNFIQNMK"
     gene            25190..25327
                     /locus_tag="NBH32_000522"
     CDS             25190..25327
                     /locus_tag="NBH32_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514154.1"
                     /translation="MMDLIENGKDANEVLKMPFHYVLSIYQNKNNDISEEKAEALIDA
                     F"
     gene            25384..29913
                     /locus_tag="NBH32_000523"
     CDS             25384..29913
                     /locus_tag="NBH32_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001799555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCD1514155.1"
                     /translation="MGERIKGLSIGLDLDSANLNRSLTEIKRNFRTLNSDLKLTGNNF
                     KYTEKSTDSYKQRIKELDGTIAGYKKNVDDLAKQYDKVSQEQGENSAEAQKLRQEYNK
                     QANELNYLERELQKTSAEFEEFKKAQVEAQRMAESGWGKTSKVFESMGPKLTKMGDGL
                     KSIGKGLMIGVTAPVLGIAAASGKAFAEVDKGLDTVTQATGATGGELKKLQNSFKDVY
                     GNFPADAETVGGVLGEVNTRLGFTDKELESATESFLKFSHITGSDGVQAVQLITRAMG
                     DAGIEADEYQSVLDMVAKAAQASGISVDTLADSITKYGAPMRAMGFEMKESIALFSQW
                     EKSGVNTEIAFSGLKKAISNWGKAGKDPREEFKKTLAEIERTPDIASATSLAIEAFGA
                     KAGPDLADAIKGGRFSYQEFLKTIEDSQGTVNQTFKDSESGSERFKVAMNKLKLVGAD
                     VWASIESAFAPVMEELIKKLSIAVDWFSSLSDGSKRSIVIFGGIAAAIGPVVFGLGAF
                     ISTVGNAVTVLAPLLASIAKADGLISFLSTKVPILGTVFTALTGPIGIVLGVLAGLAV
                     AFTIAYKKSETFRNFVNGAINSVKQTFSNFIQFIQPFIDSVKNIFKQAVSAIVDFAKD
                     IWSQINGFFNENGISIVQALQNICNFIKAIFEFILNFVIKPIMFAIWQVMQFIWPAVK
                     ALIVSTWENIKGVIQGALNIILGFIKFFSSLFTGNWRGVWDGIVMILKGTVQLIWNLI
                     QLWFVGKILGVVRYFGGLLKGLISGIWGVIKGIFTKSLSAIWNATKSIFGFLYNSVKS
                     IFTNMKNWLSSTWNNIKSNTVGKAHSLFTGVRSKFTSLWNATKDIFTKLRNWMSNIWN
                     SIKDNTVGIAGRLWDKVRNIFGNMRDGLKSIIGKIKDHIGGMVSAIKKGLNKLIEGLN
                     WVGGKLGMDKIPKLHTGTEHTHTTTRLVKNGKIARDTFATVGDKGRGNGPNGFRNEMI
                     EFPNGKRVITPNTDTTAYLPKGSKVYNGAQTYSMLNGTLPRFSLGTMWKDIKSGASSA
                     FNWTKDQIGKGTKWLGDKVGDVLDFMENPGKLLNYILEAFGIDFNSLTKGMGIAGDIT
                     KAAWSKIKKSATDWIKENLEAMGGGDLVGGILDPDKIKYHYGRTAAYTAETGRPFHEG
                     VDFPFVYQEVRTPMGGRLTRMPFMSGGYGNYVKITSGVIDMLFAHLKNFSKSPPSGTM
                     VKPGDVVGLTGNTGFSTGPHLHFEMRRNGRHFDPEPYLRNAKKKGRLSIGGGGATSGS
                     GATYASRVIRQAQSILGGRYKGKWIHDQMMRVAKRESNYQSNAVNNWDINAQRGDPSR
                     GLFQIIGSTFRANAKRGYTNFNNPVHQGISAMQYIVRRYGWDGFKRAGDYAYATGGKV
                     FDGWYNLGEDGHPEWVIPTDPARRNDAMKMLHYAAAEVRGRKASKNKRPSQLSSVNGF
                     DDPSLLLKMIEQQQQQIALLLKIAQSNDVIADKDYQPIIDEYAFDKKVNASIEKRERQ
                     ESTKVKFRKGGIAIQ"
     gene            29910..31394
                     /locus_tag="NBH32_000524"
     CDS             29910..31394
                     /locus_tag="NBH32_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HCD1514156.1"
                     /translation="MIDTIKVNNKTIPWLYVERGFEIPSFNYVLKTENVDGRSGAIYK
                     GRRLESYSFDIPLVVRNDYLSHNGIKTYDDVLNELVKFFNYEEQVKLQFKSKDWYWNA
                     YFEGPIKLHKEFTIPVKFTIKVVLTDPYKYSVTGNKNTAISDQVSVVNSGTADTPLIV
                     EARAIKPSSYFMITKNDEDYFMVGDDEVTKEVKDYMPPVYHSEFRDFKGWTKMITEDI
                     PSNDLGGKVGGDFVISNLGEGYKATNFPDAKGWVGAGTKRGLPKAMTDFQITYKCIVE
                     QKGKGAGRTAQHIYDSDGKLLASIGYENKYHDRKIGHIVVTLYNQKGDPKKIYDYQNK
                     PIMYNLDRIVVYMRLRRVGNKFSIKTWKFDHIKDPDRRKPIDMDEKEWIDGGKFYQRP
                     ASIIAIYSAKYNGYKWMEMNGLGSFNTEILPKPKGARDVIIQKGDLVKIDMQAKSVVI
                     NEEPMLSEKSFGSNYFNVDSGYSELIIQPENIFDTTVKWQDRYL"
     gene            31410..35195
                     /locus_tag="NBH32_000525"
     CDS             31410..35195
                     /locus_tag="NBH32_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514157.1"
                     /translation="MIHVLDFNDKIIDFLSTDDPSLVRAIHKRNVNDNSEMLELLISS
                     ERAEKFRERHRVIIRDSNKQWREFIINWVQDTMDGYTEIECIASYLADITTAKPYAPG
                     KFEKKTTSEALKDVLSDTGWEVSEQTEYDGLRTTSWTSYQTRYEVLKQLCTTYKMVLD
                     FYIELSSNTVKGRYVVLKKKNSLFKGKEIEYGKDLVGLTRKIDMSEIKTALIAVGPEN
                     DKGKRLELVVTDDEAQSQFNLPMRYIWGIYEPQSDDQNMNETRLSSLAKTELNKRKSA
                     VMSYEITSTDLEVTYPHEIISIGDTVRVKHRDFNPPLYVEAEVIAEEYNIISENSTYT
                     FGQPKEFKESELREEFNKRLNLIHQKLNDNISNINTIVKDVVDGELEYFERKIHKSDT
                     PPENPVNDTLWYDTSNPDVAVLRRYWNGRWIEATPNDVEKLGGITREKALFSELNNIF
                     INLSIQHASLLSEATELLNSEYLVDNDLKADLQASLDAVIDVYNQIKNNLESMTPETA
                     TIGRLVDTKTLFLEYRKKLQDVYTDVEDVKIAISDRFKLLQSQYTDEKYKEALEIIAT
                     KFGLTVNEDLQLVGEPNVVKSAIEAARESTKEQLRDYVKTSDYKTDKDGIVERLDTAE
                     AERTTLKGEIKDKVTLNEYRNGLEEQKQYTDDQLSDLSNNPEIKASIEQANQEAQEAL
                     KSYIDAQDNLKEKESQAYADGKISEEEQRAIQDAQAKLEEAKQNAELKARNAEKKANA
                     YTDNKVKESTDAQRRTLTRYGSQIIQNGKEIKLRTTKEEFNATNRTLSNILNEIVQNV
                     TDGTTIRYDDNGVAQALNVGPRGIRLNADKIDINGNREINLLIQNMRDKVDKTDIVNS
                     LNLSREGLDINVNRIGIKGGNNNRYVQIQNDSIELGGIVQRTWKGKRSTDDIFTRLKD
                     GHLRFRNNTAGGSLYMSHFGISTYIDGEGEDGGSSGTIQWWDKTYSDSGMNGITINSY
                     GGVVALTSDNNRVVLESYASSNIKSKQAPVYLYPNTDKVPGLNRFAFTLSNADNAYSS
                     DGYIMFGSDENYDYGAGIRFSKERNKGLVQIVNGRYATGGDTTIEAGYGKFNVLKRRD
                     GNRYIHIQSTDLLSVGSDDAGDRIASNSIYRRTYSAAANLHITSAGTIGRSTSARKYK
                     LSIENQYNDRDEQLEHSKAILNLPIRTWFDKAESEILARELREDRKLSEDTYKLDRYV
                     GLIAEEVENLGLKEFVTYDDKGEIEGIAYDRLWIHLIPVIKEQQLRIKKLEESKNAG"
     gene            35185..35337
                     /locus_tag="NBH32_000526"
     CDS             35185..35337
                     /locus_tag="NBH32_000526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514158.1"
                     /translation="MQDNKQGLQANPEYTIHYLSQEIMRLTQENAMLKAYIQENKENQ
                     QCAEEE"
     gene            35384..35671
                     /locus_tag="NBH32_000527"
     CDS             35384..35671
                     /locus_tag="NBH32_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514159.1"
                     /translation="MAKEIINNTERFILVQIDKEGTERVVYQDFTGSFTTSEMVNHAQ
                     DFKSEENAKKIAETLNLLYQLTNKKQRVKVVKEVVERSDLSPEVTVNTETV"
     gene            35729..36025
                     /locus_tag="NBH32_000528"
     CDS             35729..36025
                     /locus_tag="NBH32_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2951 domain-containing protein"
                     /protein_id="HCD1514160.1"
                     /translation="MGWFKKHEHEWRIRRLEENDKTMLSTLNEIKLGQKTQEQVNIKL
                     DKTLDAIQKEREIDEKNKKENDKNIRDMKMWVLGLVGTIFGSLIIALLRMLMGI"
     gene            36175..36351
                     /locus_tag="NBH32_000529"
     CDS             36175..36351
                     /locus_tag="NBH32_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HCD1514161.1"
                     /translation="MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIAL
                     IGLVIKLIELSNKK"
     gene            complement(36404..36511)
                     /locus_tag="NBH32_000530"
     CDS             complement(36404..36511)
                     /locus_tag="NBH32_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514162.1"
                     /translation="MIFILWCVNLYIEKGQHAQTCYPNEPVKKTVAILD"
     gene            36563..36817
                     /locus_tag="NBH32_000531"
     CDS             36563..36817
                     /locus_tag="NBH32_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1514163.1"
                     /translation="MINWKIRMKQKSFWVAILSAIFLFAQNIAKAIGYDIQVYTEQLT
                     DGLNAILGFLVLTGVIQDPTTKGIGDSHQALEYEEPRRKY"
     gene            36829..37584
                     /locus_tag="NBH32_000532"
     CDS             36829..37584
                     /locus_tag="NBH32_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1514164.1"
                     /translation="MKTYSEARARLRWYQGRYIDFDGWYGYQCADLAVDYIYWLLEIR
                     MWGNAKDAINNDFKNMATVYENTPSFVPQIGDVAVFTKGIYKQYGHIGLVFNGGNTNQ
                     FLILEQNYDGNANTPAKLRWDNYYGCTHFIRPKYKSEGLMNKITNKVKPPAQKAVGKS
                     ASKITVGSKAPYNLKWSKGAYFNAKIDGLGATSATRYGDNRTNYRFDVGQAVYAPGTL
                     IYVFEIIDGWCRIYWNNYNEWIWHERLIVKEVF"
     gene            37793..38284
                     /gene="sak"
                     /locus_tag="NBH32_000533"
     CDS             37793..38284
                     /gene="sak"
                     /locus_tag="NBH32_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylokinase"
                     /protein_id="HCD1514165.1"
                     /translation="MLKRGLLFLTVLLLLFSFSSITNEVSASSSFDKGKYKKGDDASY
                     FEPTGPYLMVNVTGVDGKGNELLSPHYVEFPIKPGTTLTKEKIEYYVEWALDATAYKE
                     FRVVELDPSAKIEVTYYDKNKKKEETKSFPITEKGFVVPDLSEHIKNPGFNLITKIII
                     EKK"
     gene            38934..39269
                     /locus_tag="NBH32_000534"
                     /pseudo
     CDS             38934..39269
                     /locus_tag="NBH32_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
     gene            complement(39364..39813)
                     /locus_tag="NBH32_000535"
     CDS             complement(39364..39813)
                     /locus_tag="NBH32_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis-inhibiting protein CHIPS"
                     /protein_id="HCD1514166.1"
                     /translation="MKKKLATTVLALSFLTAGISTHHHSAKAFTFEPFPTNEEIESNK
                     KMLEKEKAYKESFKNSGLPTTLGKLDERLRNYLKKGTKNSAQFEKMVILTENKGYYTV
                     YLNTPLAEDRKNVELLGKMYKTYFFKKGESKSSYVINGPGKTNEYAY"
BASE COUNT        14942 a         5680 c         7766 g        11483 t
ORIGIN      
        1 attcatcact attgcaaagt gtgaattaga gaattcttta ttaacgttta taccgaaatc
       61 tacaaaaact atttctcctt gtttaaactt tggataaaaa cctttatggt ttttttcacc
      121 ttcaaatctc ttgagtaaat agtgaatatc tgaatctaat tttttaaatt ttggatttcc
      181 agaagttttt aatttattaa tgcgtttttc tatattatgc gtcatcattt ctcctttatt
      241 ctcgctcaca ctctcaccac cattcaacgt ctacacttgt agacgttttt tgattagtaa
      301 aatcataatg aatcttcttt ggttaactta tcgccatcta ttttttgtga aataaattcc
      361 aagtatttac acgcattatg tgacgataaa tctttaggta actcataagt gaatggttga
      421 ttaccactag ttaaaacttc atatactata gtttcttttt ttattttgca attagttatt
      481 ttcattataa acttcctttc aaacactgct gaaatagacg tcttttatat taaagcgcca
      541 cacaggcgct gttaatcaca atttagttct atcagtgatt ttagactcca taactctttg
      601 atgtgattct ttagcttctc gaatcatatc tttaaattct tgactgtcta taaaagcttt
      661 agcctcttct atttgctctt gagtaagctc tttaccacca gtattgatgt gtaagtgttc
      721 aatttcttta taagtactca ttttttcgac tcctgttctt caagttcact tttagttata
      781 ggtaaaccat tgttcaatct ataagtcagt tcttcttctg tataaaaggg gatttcaacc
      841 atttcccact cttcaatgtt aatgtcaact tctttaaaat ccatgttaaa ccctcctgtg
      901 aaatgaattt tctattattt atagtaatta cttataaaaa tacaatcttt acctatctca
      961 aactttgtat tctaaatgta ctcgtaatcc atagtctgat tctttagtaa cgattttctc
     1021 ttctaaataa tctaaagttt tatacttacc accattaata tatgcgttac aagaaacgat
     1081 gttgtccata tgattgacta atcttgaagc atactctcta ggtacatatc caacgtgaaa
     1141 ttcagagtat tcatttgaaa tcataacttt tatcgcgttt tcatcataag gattatccgg
     1201 ttctttttgt aaaaatacac caggaataac ctcgtaatca gaaatttcat acaccttgtc
     1261 ttcataaagt aattcttctt taagttcatt tcctttcaaa tcactatata agaaaaagaa
     1321 atcgtcgtta tttttcattt tcttgataag tttctttaat tcttttctac gaccttcata
     1381 atttaatcct acgacgtcga aaatttcaac tttagtttgt tcatcatcat taataggtag
     1441 acaatcattc gagataattg tttccttatt cttagataat tgcatataag tttttaaaat
     1501 tgagatgaat cctgttaaag gagagtttgt tacgaaataa actgttaatt ttctattatc
     1561 gtttaatgtt aaaaaagctt ggtttttcca aatagtaaca acagtgttat aatctatcac
     1621 ctctgataat gagattttga atatataatc ttcttctttc cttataaaac aaatctcttc
     1681 atgtgaaatg aatatagaac ccattctcct cttgttttcg tcgaatttta tgtcgcaact
     1741 gtcgctgatt attggttcaa agtaactgta ttgatctgat aatatttttt catcttgctt
     1801 tctaggtttc attttactac ctcctataaa ataacttttc caactaacct cacactttcg
     1861 ttatcataaa aatataaatc tttatacttt ttatttaaag aaaccaacgt taatctatta
     1921 tcttctacat aaactttctt tacgtaagca tctccattta taataaagac gcctatttgt
     1981 ccatctttga tagtgtgaga tttttcaatg aatataattt gtccgttttt aaataacggc
     2041 tccattgagt ctccatttac ttttaaagct atatcatgtg cggggacata acctcttacg
     2101 aattcttttg aaataggctc gttatataat ctttcgccaa taccagctga cgcacaacca
     2161 tatatatcca cttcggattt ttcttgaatg taagaattga aatctaccag attatcactg
     2221 tcattatttt gttcttctaa ttgattagtc gcatatttta gtacattgct ttgtcttgga
     2281 ggcgtgagtt tactgtatat ggaagtgatg tcgttatttt caatttttct attcttagaa
     2341 atatcaaacc ccataagcca cgcttcgtta acgtttaaag cctttgctag ttcaaagact
     2401 ttgtcttgtt tcgcttcata ttttccgttt aaataatcgc taattgagtt tctaccaata
     2461 ccagtccttc ttgatagctc tgattgagat atcttccgtt cagacataat ttgctttaat
     2521 ctatccttaa aactgttcat atttctgaac acctcctaag aacataatac tacgtacaat
     2581 gacgattatc aataattttt aacaaatatt gtacagaaaa atgtatttta tgtgttgact
     2641 tatttgaaca aaggtgtttt aattgatttg tacagaaaac cgaacaagaa gggaggtgag
     2701 tttatgatat acaatttcga ttatagtttg ctgtacgaaa gaatggcaga gtatagatat
     2761 agccaaagtt ctttagcgaa cgcaatccct atttcaagga catctattaa tcacaagttg
     2821 caaggaaaaa atttatttac acaatgggaa ataaaacgaa tctgtgaatt attagaaatc
     2881 ccaccaacaa aagtaggtag atattttttt gaacaaaatg tacagaaacc tgtacaaatg
     2941 tcgtaacagg aggaaactat ggaacaaatc acattaacca aagaagagtt gaaagaaatt
     3001 atagcaaaag aagttagaga ggctataaat ggcaagaaac caatcagttc aggttcaatt
     3061 ttcaacaaag taagaatcag ccataacgat tttgatgaaa ttaataaaaa gtttgcttat
     3121 acagaacgtt taagaggtgc tgacaatctc ggcttaggac atccattatc tttgaagaaa
     3181 tatcaacacg gaataggatg ttatgaaaat tacaaagcat acgctagtga aattcatgac
     3241 cacattagaa aacttacatt atcagctttt ggtgtaacgc ttaattctga tttaaaagag
     3301 agtgaatacg atgaagctag cagaatgtat gacatgttga agaactttta tttatatcgt
     3361 taccaaaaac gaattgaaac cttgtcaatt gaagatttcg aataaaggag gaactacaaa
     3421 tgttacaaaa atttagaatt gcgaaagaaa aaaataaatt aaaactcaaa ttactcaagc
     3481 atgctagtta ctgtttagaa agaaacaaca accctgaact gttgcgagca gttgcagagt
     3541 tgttgaaaaa ggttagctaa attcaacggt aaggatttgc cctgcctcca cacttagagt
     3601 ttgagatcca acaaacacat aagttttagt agggtctaga aaaaatgttt cgatttcctc
     3661 ttttgtaaca gtttcaattc cttcatatcc tggaaaaaca attttcttta aatccgaaac
     3721 atgttttttt gaaccatcct ttaaagtaac tagaagtttc atacttatca cctccttagg
     3781 ttgataacaa cattatacac gaaaggagca taaacaatat gcaagcatta caaacaaaat
     3841 cgaacatagg agaaatgttc aatattcaag aaaaagaaaa tggagaaatc gcaataagtg
     3901 caagagagtt atataaagct ttggaagtta aaaagcgttt tagcgcttgg gcagaaatta
     3961 acttgaagca tttcaaagaa aatagggatt ttacaagtgt acttacaagt acggttgtta
     4021 ataacggagc tgtaagacaa ctagaagatt atgctttaac acttgatgta gctaaacatg
     4081 ttgcaatgat gtcaggtaca gaaaaaggtt ttgattttag agagtacttc atccaagttg
     4141 aaaaagcatg gaatagccca gaaatgatta tgcaacgtgc tttaaaaatt gctaacaaca
     4201 caatcaatca attagaaaca aagattgaac gtgataaacc aaaaattgta tttgcagatg
     4261 cagtagctac tactaagaca tcaattttag ttggagagtt agcaaagatc attaaacaaa
     4321 acggtgtaaa catcgggcaa cgcagattgt ttgagtggtt acgtcaaaac ggattcctta
     4381 ttaaacgcaa gggtgtggat tataacatgc ctacacagta ttcaatggaa cgtgagttat
     4441 tcgaaattaa agaaacatca atcacacatt cggacggtca cacatcaatt agtaagacgc
     4501 caaaagtaac aggcaaagga caacaatact ttgttaataa gtttttagga gaaaaacaaa
     4561 catcttaata ggagaacact atggaacaaa tcacattaac caaagaagag tgtgtcgaac
     4621 aatgcatcaa taaagactta aaacttttag attatcgagt tcaacaaatt ttagaaggtg
     4681 ttctatcaga aagtaccaca tacggtgatg caagaaataa attagaaaca ttgaaaatta
     4741 ttgctgaatc tcattttaaa accgaacatg cttcagttat ttacaaatta gcattgaaaa
     4801 agttagacaa aaaaatcaac gccactccaa ttaaagagtg acgaaaaagg aggatttcaa
     4861 atgtttaaga ttttaaatga tataaaaact tctttaaaaa accatccttg gggttggaaa
     4921 gagcacttac cttatttact gatgttaact ctgtcacttg tggctctgat tttcggtgtt
     4981 ctgtccgcga ttctatgata acaggtttta tatagattcc ttacctcctc tctgtaggag
     5041 ataacaatat tatacacgaa aggaatgata gaaatgccac atgtattaaa cgtaaccgtt
     5101 ccaatacctg aaacacacgt gcttatcaca aaagatgaat atgaagagtt aatagcttac
     5161 tcattagacc ctgtatggaa catgagcgac ttaaagaaga aattaaaaat tgcatctgat
     5221 gagactatca aggacagatt actatttcat cctagatttg aaaaagaact aagagcgcaa
     5281 ggaattgtgc attacccaga tgagaatttt aatcgctgga gatttaacgc aagaaagatg
     5341 aataaattcg tcgatgagca tttcaatgaa atatataagg agagaataaa atgagcaaca
     5401 tttataaaag ctacctatta gcagtattat gcttcacagt cttggcaatt gtacttatgc
     5461 cgtttctata cttcaccact gcatggtcga ttgcgggatt cgcaagtatc gcaacattca
     5521 tattttataa ggaatacttt tatgaagaat aaaaaaactg ttactcacgg caatgagtaa
     5581 cagtctaaac aattagaaaa ttaatgcata ttcaatataa aacgaaataa aggaagtgtc
     5641 aacaatgtac tacaaaattg gcgatgtatg tcaaaaagta attaatgtag acggattcga
     5701 ttttaaatta gcagttaaga aacaagatta cagcattcta gtgaatgtct tagatttaga
     5761 agatagattt atcgacggta taaatataac agatgagaat gatctataca cagcattaga
     5821 catattaaat caatctattt atgaatggat tgaagagaac acagacgaaa gagacaggct
     5881 aattaactta gtcatgagat ggtaggtata agcatgagag atacagaaag aaatatattg
     5941 aatattttta agacgttatt cgacgaatat actttgtcaa accaacgagc attattggaa
     6001 attgaacgta atcatcacgg atacttatcg attaatttct tacactatca cgacagttac
     6061 aaaacaaaca ataagcttgt gcagatacat gaaatcaatc cagacagcca tgaacgaata
     6121 aaaaatttaa ttatcgaggt gctaagaggt catcggaaga ttaaaaaagg agcatgagga
     6181 aagatatgaa aataaataag ttaactatat caaactttgc tggaatcaaa gaagaaagct
     6241 ttaactttaa cggcaaagat gcaaaaatat acggcaataa tgcaacgggt aagactacga
     6301 cagcaaccgc attacaatgg ctgcttttcg ataaaggttt ggacggatca accaaatcat
     6361 ttaaccctgt atctttaaac gaaaaaaacg aagaaaatta tgagttaatt ccgactgttt
     6421 tcgcagaatt tgaaatcgac ggaaaaatta cgacttttaa aaaagagtca catcctaaat
     6481 acacaataaa tcaaaaaacg aatcgcaagg aatactcacg aagtcgaacg aagaaacaat
     6541 atatcaatga tgaatcaata aaagtaaagg attataaagc tcgtattgat gaactgattg
     6601 atgaagatgt attcaagtta attacgaacc ctcaagcatt taacttacta gattggaaga
     6661 agcgaagaag tttgttgttt gaaattgcta aaccaatcaa tgatgaggat gtcattaaaa
     6721 caaatgatga ttttaaagaa ttaaataata ttcttggtga tcatgaaatt gaaacaaaga
     6781 aaaaaattct tactgacaag ataaaacaga ttaacaaaga catcaaagat attccgatac
     6841 gtattaatca aacacaacaa aataagcagg atgtaccaga attcgataat gatagatacg
     6901 caattatcaa acaagaaatt gagcaacttg aaaatgagcg tatagatatt caaaacggta
     6961 aggaagaaat taatttgcgt aatcaattag ctgataaaca atcagaattg aaacgcatag
     7021 aagacaataa cagcgcaagt aatgagaaca aaatccatgc tttaacaaat gaattacacg
     7081 ttgaaaatgg aacggtagca aaccttaaaa cgagattaaa gcaaaacaaa caacaaatca
     7141 cgcatgaaga aaatagacgt aatcaattat tagaaaatca taaaggatta aaaagtgatt
     7201 tagaaaaagc taaaaatcaa aaatttgaat atcttgatga caatgtatgt agttgttgtg
     7261 gtcaacagtt accagctgaa caagtgaatg aggcaagaga aaaagcattg cagaaattca
     7321 atgctggcaa atcgaaagaa ttagaaacaa tacaaacatc tatcaatcac attatttcag
     7381 aaggcaagaa aataaagcca atcatcgaga agttagagga tgacaataat aatcttcaaa
     7441 ttaaaatcaa cgaagcagaa gagcgttcag caagaataca aaacaaaatt aataagttga
     7501 aaacgactca tgttgacgtt acacaaactg acgaatacaa agcagtaatg ttagagataa
     7561 acgagattaa tcaaaaacgc tcgaacatta ggaaaactat tcaagataac gtttcaggaa
     7621 tagatgacaa aataagcgaa cttactcaag aaaaatcaga aattgaagtg tcaagatcaa
     7681 tcgaaaaatc aaataaacat ctagatgatg ttatttctga attaagaaat gaagaagata
     7741 gattattgga tgaaaaagaa aagtattcac atgaccttta tatcttaaaa gaatttacaa
     7801 caacaaaagt caaaatgctt actgaaaata tcaataatga atttgagatt gctgaattta
     7861 agttattcaa taccttagtt aacggcgaat tagaagaaac atgttccaca acggttaacg
     7921 gcgtcgaata cgacagcggt ttaaataacg cctcaagaat taatgttggc ttagatatca
     7981 tcaatacact gtcaaaacat tttaaagtta cagcgccaat atttattgat aatgctgaat
     8041 cagtaacaga gcttatcaaa acagaatcac aacaaattca attgatagta aatgaacaag
     8101 ataaaaaatt aagaatggag actatataaa atgactgaaa ataataaatt acaaactatt
     8161 gaacaacaat tagtacaaga aaagaacgta tctgacaacg tattaaacaa agtgagagtt
     8221 ttagagtcac aaggcaattt ggaattgcca aatgattatt caccaagtaa tgccatgaaa
     8281 caagcatggt tacaaatcag ccaagataac aaattaatga gttgtaacga tacaagcaaa
     8341 gcaaatgcct tattagacat ggtaacgcaa ggtttaaatc cagctaaaaa tcaatgctac
     8401 tttattcctt acggcaacaa aatgcagtta caacgtagct atcacggtaa tgtaatgatg
     8461 ttaaaacgtg atgcaggtgc tcaagatgtt gttgctcaag tgatttataa aggcgataca
     8521 ttcaagcaag aaatgggagg aacaggacgt atcaaagcga ttaaacacga acaagacttc
     8581 tttaacatcg acaaagaaaa cattatcggt gcgtactgca caatcgtatt taatgatgga
     8641 cgagataact atattgaagt catgactatt gaacaaatta aacaagcatg gatgcagtca
     8701 tcaatgatta aagatgaaaa agcattacaa aattctaaaa cacataataa tttcaaagaa
     8761 gaaatggcta aaaaaacagt tatcaataga gctgctaaac gttatatcaa cacatcaaca
     8821 gatagcaatc ttttcaaata cgcacaagaa tccgaacaac gtcaacgcaa agaagtgttg
     8881 gacgcagaag ttgaagaaaa tgcaaatcaa gaacaattgg actttgaaca accagttctc
     8941 gaagaagcac aatacacaga attagaaaat gataagccta ttgatgtatc tgactttgaa
     9001 gaaataaaag aacctgcaac agaaaaagaa agcgaagaag agccatttta attgaaacaa
     9061 tagcaactgg ttcaagtggt aactgctacg tcttaaatga tggacgtact acgttactac
     9121 ttgagtcagg tataaaattt gaacgtgttc aaaagcattt taaatataaa acaagacata
     9181 tagcagggtg tcttatcaca cacgaacatg gtgatcatgc aaagtacaca aagcagtttg
     9241 tcgacaatgg tgtaatcagc tatatgactg ctggaacaca acaagctatg aattttgaaa
     9301 gtcatcgctt atgcacgatt aaggcaaagc aagagctgcg aataggcaca tggtcaattc
     9361 taccgtttga catcgaacat gatgctaacg agcctgtggc tttcttatta caaagtacat
     9421 taggttataa ggttctgtat gttactgata caaagtattt gaaatacaaa tttaacggca
     9481 ttacgcacat gatgttagaa gttaattata tctatgaaca aatgcaggaa aacataaaaa
     9541 acggcagtgt gcacagcaca ttagcaaaca gaattatgga gtctcatttt agcttagaac
     9601 atgctatcgg aatgttaaaa gcaaatgatt taactagact cgaagaaata catttaattc
     9661 atttaagtag ccaaaattca aatgcaaaat acattaaaag tgaaatacaa aaagtgacgg
     9721 gcgtgcccgt ttatgttgga ggtttataaa tgataaacag aacaatatta gttggtcgtt
     9781 taactagaga cccagaatta agaaccactc aaagtggtgt aaatgtagca tcattcacat
     9841 tagcagttaa ccgtacattt acaaatgcac aaggagagcg cgaggcagac tttattaata
     9901 tcatcgtatt taaaaaacaa gcagagaacg ttaataaata cctatctaaa ggatcgttgg
     9961 cgggcgtaga tggcagatta caaacgcgta actatgaaaa caaggaaggt caacgtgtat
    10021 atgttacgga agttattgct gatagtattc agtttttaga accgaaaaac tcaaatgaca
    10081 ctcaacaaga tttataccaa caacaagtac aacaaacacg tggacaatcg caatattcaa
    10141 ataacaaacc agtaaaagat aatccgtttg cgaatgcaaa tggtccgatt gaaatagatg
    10201 acaatgattt accattctaa tttaaccggt ttgaaagtga ggtgtgtata tgactggttg
    10261 gataaaactt catagaaaat tattagattc gcctattttt cagaacgaaa agttattcaa
    10321 agtatttgca tattgtctta tgaaagctag tcataaggat catacacagc ttgttggcag
    10381 gcgggttgtc gaattagaaa aaggtcaatt tgtgttcggg agaaagcgag caagcgaaga
    10441 gttacgtctc aaagaatcca cagtaagaga ctacataaag cttttagaaa atcttggaac
    10501 tatcgtcgta aagtccgaca acaaattttc tgttataacc gttgtcaatt gggcgattta
    10561 tcaaagtatg gaagaaaatt ccgacagcaa aaacgacaac aaatcaacaa caaatggaca
    10621 acaaatcaac aacaaatcaa caacaaatgg acaacaaatc aacacaaaca agaatgtaaa
    10681 gaatggggat aatgtaaaga atgatgagaa tgagaagaag aaggcagctg ccttcgactt
    10741 cttccaagat aacggattcg gtttcataac tccttacaat ttagacgatt taaattatta
    10801 tcttgattca tttgaaaatg attcagatca aatagttacc gcatcactta aaatcgctaa
    10861 agacagaaat aaagttactt ggggatatgc taaaagcatt ttgaatacat ggcttaatgc
    10921 aaacttgaaa tctattgaac aaatacgtgc atttgaaaag caacaactgg aaagcaaaaa
    10981 gcaaactaat aaaccttatg ttaaaccatc gaaagaaaaa acacccaaat ggctcacaga
    11041 cagcacgaga gaaacgaaaa cgccggaagt agatgaaaac cttgagaaag acagagaagc
    11101 ttttattaag cgtctaaata gcaaatggga gtgattgaaa atggatgcat ttgataaata
    11161 ctatctattt gatcatgacg gcaacaaaat gttttcagtt acaccacatt ttaaagatgg
    11221 tcggcattta gttgttggaa taaaagaaac aaaatttaat ggtcgtcgtt ggtatttaga
    11281 cgattatgaa ttaaatacac ttattgataa tgaacaaatg gagttaggac accaaacaag
    11341 cttatttgaa tatatatgag ggattacatg gagatagaaa ttaaatttaa tgaagtgttt
    11401 aatgcgccga tggggtcgcc ccgtccacgc tttcgtaaaa caggtagatt tgttcaaact
    11461 tacatgccaa cgtcttacac aaagcataaa gcgtatatac aagggcaaat gcctaagtta
    11521 aatctagagc gcgcactaaa aatcgaatta gacttttact ttccattgct taaatcgtgg
    11581 tcgaagaaaa agaaaagcga aatggttggg cagtataaag tgactaatcc ggatatcgac
    11641 aacttaatta aaacggtatt agatgcttgt aatggccatg tatggaaaga cgataaccaa
    11701 attacagaaa taactagctc aaagcgttat ggaattgagc ccaaaataat catacgaata
    11761 gaagaaatat aagaggtgga taaaatggcg agaaaagcaa gaattgtaac aataaacgat
    11821 aaaccttata ggttcagtaa atttgaaatg gaattaatag aaagtcacgg tataaccgct
    11881 ggaatggttt ctaagagagt aaaagacggt tgggaactac atgaagcaat ggacgcacca
    11941 gaaggtacgc gtttaagcga gtacagagaa aagaaaacaa tagaaagact ggaacaagct
    12001 agactcgaac gcaaattgga aagaaagcaa aagaaagagg ctgagctaag aagaaagaag
    12061 ccacatttgt ttaatgtacc acagaaacat ccaagaggac gttatgcgtg ctacctgatg
    12121 gaaaacgaca tattcgtgaa agttaagaag tagatcatga cagataacgc acgcaaagaa
    12181 tacctaaatc aattctttgg atctaagaga tatctgtatc aggataacga acgagtggca
    12241 catattcatg tagtaaacgg cacttattac tttcatgggc atatcgtacc aggttggcaa
    12301 ggcgttaaaa agatgtttga tacagcagaa gagctcgaaa catatataaa gcaacatggt
    12361 ttggaatacg aggaacagaa gcaactaact ttattttaga ggagatataa aaatgaaaat
    12421 caaatttaaa aaagaaatga cattagatga attaattaag tgggcatggg agaaccctga
    12481 attagtaaga ggagagaaat tttatgcgca aggcaagagt aatgaaacat atgtgtattt
    12541 ccatctttac gacggaagaa agtgcatctt aagagaatat atatcagccg atgacacttt
    12601 tgaagtcgaa tatgaagagg aaatcacgga agaaactgta ataccgaagt tagtaaaaat
    12661 gtacaaagac ggaaaaatgt ccgtttacaa tgactattcg attaagcgtt ctttgcttta
    12721 ttcccctaaa gcatactata tcttaaacga cgacctaact atgacgttaa tctggaaaga
    12781 tggggagttg gtagaatgat gcaaacctat aaagtaagtc tttgtatcaa gttcttagcg
    12841 tctaaatgta attataaatt aaaaaagcat tattttgtgc aaagtacgaa tgaggaagaa
    12901 gccacgaata cggtattaaa actgactcgt aaaaagctcc cgttccaaac tgcaagcata
    12961 gaggtcgaaa aagtggaggt agtagtatga tgccgaaata tcgagtgtgg gacgaatata
    13021 caggaagaat acacgatgtt gtaggattcg acttcattga gactgaagtt cactatgaaa
    13081 actacgcgga agcagaagct ttaatacatg caagagattt taaagatgta gaacttatgc
    13141 aaagtacagg acttaaagac aaaaacaaca acgaaatata tgcgggagat atagttgagt
    13201 ttgaagatga aatattagag atgccagacg atgaatctgt aataggaaca attaatagag
    13261 cagtaatatc tattgatgtt gtaaatggta ttcaattaaa agattttatg tttgagggcg
    13321 cagtctccga aaatgattac tttgagtata tagacataaa atccttcctt agatatgact
    13381 gtgaggttaa aggcaacata tttgaatcat cacatttatt ggaggtaaca gaatgaacta
    13441 tgaaacaggg gtccaactag gtgtaatgga cgctaggttg aagaagatga gaaaacaacg
    13501 tgatgagtac aagaagcaac gtgatgagct tattggggat ataggtaagt taagagaacg
    13561 caacaaagag ctggagaaga aagcaagtgc atgggatagg tattgcaaga gcgttgaaaa
    13621 agatttaata aacgaatttg gcaaagatgg tgaaagagtt aaatttggaa tggaattaaa
    13681 caataaaact tttatggagg aagacactaa tgaataaccg tgaacaaatt gaacagtccg
    13741 ttataagtgc tagtgcgtat aacggcaatg acacagaggg attactaaaa gagattgagg
    13801 acgtatataa gaaagcgcaa gcgtttgatg aaatacttga gggaatgaca aatgctattc
    13861 aacattcagt taaagaaggt attgaacttg atgaagcagt agggattatg acgggtcaag
    13921 ttgtctataa atatgaggag gagcaggaag atgaaaaaat ttaatgttca aatcacatac
    13981 actggcatga ttgaagagac tatcgaggct gaaagtttag acgaagcaga atttgaggct
    14041 catgatattg cgagaatgga agtgccattt gattgtgatg aatttgaaat taatgtagag
    14101 gtggaacagg aaaatgacta acacattaca agtaaaacta ttatcagaaa atgctagaat
    14161 gcccgaacga aatcataaga cggatgcagg ttatgatata ttctcagctg aaactgtcgt
    14221 acttgagcca caagaaaagg cagtgattaa aacagatgta gctgtaagca taccagaggg
    14281 ctatgtcggg ctattaacta gccgtagtgg tgtaagtagt aaaacacatt tagtgattga
    14341 aacaggcaag atagacgcgg gatatcatgg caatttaggg attaatatca agaatgataa
    14401 tgaaacgtta gagagtgagt atataagtaa ctttggacgt agtccttctg gtatagacgg
    14461 acaatatgcc ctactacctg taacagataa aattttatgt atgaatggta gttatgtcat
    14521 aaacaaaggc gacaaactag ctcaattggt tattgtgcct atatggacac cggaactaaa
    14581 gcaagtggag gaattcgaga gtgtttcaga acgtggagca aaaggcttcg gaagtagcgg
    14641 agtgtaaaga catcttagat cgagttaagg aggttttggg gaagtgacgc aatacttagt
    14701 cacaacattc aaagattcaa caggacgtaa gcatacacac ataactaaag ctaagagcaa
    14761 tcaaaggttt acagttgttg aggcagagag taaagaagaa gcgaaagaga agtacaaggc
    14821 gcgaaatgcg ccagttgatg gagcgaccaa cttaaacgat atcaaatcaa atattggtat
    14881 ctttcacgtt gaaaaagtcg aaccaaacga gggtatggtg gatattaata ttgagacaat
    14941 gaaaccattc gaggaggcag atgatgatta acatacctaa aatgaaattc ccgaaaaagt
    15001 acactgaaat aatcaaaaaa tataaaaata aaacacctga agaaaaagct aagattgaag
    15061 atgatttcat taaagaaatt aatgataaag acagtgaatt ttacagtcct atgacggcta
    15121 atatgaatga acatgaatta agggctatgt taagaatgat gcctagttta attgatactg
    15181 gagatgacaa tgatgattaa aaaacttaaa aatatggatt ggttcgatat ctttattgtt
    15241 ggaatactgc gattattcgg cgtaatcgca ctgatgcttg ttgtcatatc gcctatctat
    15301 acagtggcta gttaccaaaa caaagaagta catcaaggga caattacaga taaatataat
    15361 aagagacaag ataaagaaga caagttctat attgtattag acaacaaaca agtcattgaa
    15421 aactccgact tattactcaa aaagaaattt gatagtgcag atatacaagc taaattaaaa
    15481 attggagata aagtagaagt taagacgatt ggatatagaa tacacttttt aaatttatat
    15541 ccggtcttat acgaagtaaa gaaggtagat aaaaaatgat taagcaaata ctaagactat
    15601 tattcttact agcgatgtat gagctaggta agtatgtaac tgagcaagta tatattatga
    15661 tgacggctaa tgatgatgta gaggcgccga gtgattacgt ctttcgagcg gaggtaagtg
    15721 agtgatgtgg attactatga ctattgtatt tgctatattg ctattagttt gtatcagtat
    15781 taatagtgat cgtgcaaggg agatacaagc gctcagatat atgaatgatt atctacttga
    15841 tgaagtagtt aaaactaaag gatacaacgg gttaaaagaa tacaggattg aattaaagcg
    15901 aatgaataac gatattaaaa agtaatttat attatcggag gtattgcatt gaatgataaa
    15961 gattgagaaa cacgatatca aaaagcttga agaatacatt cagcacatcg ataactatcg
    16021 aagagagttg aagatgcgag aatatgaatt acttgaaagt catgaaccag ataatgcggg
    16081 agctggcaaa agtaatttgc cgggtaaccc gattgaacga tgtgcaataa agaagtttag
    16141 tgataacagg tacaatacat taagaaatat agttaacggt gtagatagat tgatagatga
    16201 aagtgatgag gatacgcttg agttattaag gtttagatat tgggattgtc ctattggttg
    16261 ttatgaatgg gaagatatag cacattactt tggtacaagt aagacaagta tattacgtag
    16321 aaggaatgca ctgattgata agttagcaaa gtatattggt tatgtgtagc ggacttttac
    16381 cctatgtaag tccgcattaa aacagtttat tatgttagta tcagattaat atttaaagtt
    16441 attaaatgct aatacgacgc atgaacaaga ggcgcatcac tatgtgatgt gtctttttat
    16501 ttatgaggta tgaacatgtt caaactaatt gtaaatacat tactacacat caagtataga
    16561 tgcgtcttga tactacttaa gttatataag gtgaaacatt atgatgacta aagacgaacg
    16621 tatacgattc tataagtcta aagaatggca aacaacaaga aaaagagtac tagaaagaga
    16681 taattatgaa tgtcaacaat gtaagagaga cggcaagtta acgacatatg acaaaagcaa
    16741 acataagtcg ttggatgtag atcatatatt atcgctacaa catcatccgg agtttgctca
    16801 tgacttaaac aatttagaaa cactgtgtat taaatgtcac aacaaaaaag aaaagagatt
    16861 tataaaaaaa gaaaataaat ggaaagacga aaaatggtaa atacccccgg gtcaaaaaaa
    16921 tcaaaagcga tcaaaatact tggggaacgg ttagggagta aacttcgcga taattttaaa
    16981 aatccatgta taaccccccc tcttataacc attttaaggc aggtgatgaa atggagatta
    17041 tagtcgatga aaatttagtg cttaaagaaa aagaaaggct acaagtatta tataaagaca
    17101 tacctagcaa taaattaaaa gtagttgatg gtttaattat tcaagcagca aggctacgtg
    17161 taatgcttga ttacatgtgg gaagacataa aagaaaaagg tgattatgat ttatttactc
    17221 aatctgaaaa ggcgccacca tatgaaaggg aaagaccagt agccaaacta tttaatgcta
    17281 gagatgctgc atatcaaaaa ataatcaaac aattatcgga tttattgccc gaagagaaag
    17341 aagacacaga aacgccatct gatgattacc tatgattagt aataaatacg ttgatgaata
    17401 tataaatttg tggaaacaag gaaagataat tttaaataaa gaaagaattg atctctttaa
    17461 ttatctacaa aaacatatat attcacgaga tgatgtatat tttgatgaac agaaaatcga
    17521 ggattgtatc aaatttattg aaaaatggta ttttccaaca ttaccatttc aaaggtttat
    17581 catagctaat atatttctta tagataaaaa tacagatgaa gctttcttta cagaatttgc
    17641 tattttcatg ggacgtggag gcgggaaaaa cggtctaata agtgctatta gtgattttct
    17701 ttctacgccc ttacacggag ttaaagaata tcacatctcc attattgcta atagtgaaga
    17761 tcaagcaaaa acatcgtttg atgaaatcag aaccgtttta atggataaca aacgaaataa
    17821 gacgggtaaa acgccaaaag ctccttatga agttagtaaa gcaaaaataa taaaccgtgc
    17881 aactaaatcg gttattcgat ataacacatc aaacacaaaa accaaagacg gtggacgtga
    17941 ggggtgtgtt atttttgatg aaattcatta tttctttggt cctgaaatgg taaacgtcaa
    18001 acgtggtgga ttaggtaaaa agaaaaatag aagaacgttt tatataagta ctgatggttt
    18061 tgttagagag ggttatatcg atgcaatgaa gcacaaaatt gcaagtgtat taagtggcaa
    18121 ggttaaaaat agtagattgt ttgcttttta ttgtaagtta gacgatccaa aagaagttga
    18181 tgacagacag acgtgggaaa aggcgaaccc aatgttacat aaaccgttat cagaatacgc
    18241 taaaacactg ctaagtacga ttgaagaaga atataacgat ttaccattca accgttcaaa
    18301 taagcccgaa ttcatgacta agcgaatgaa tttgcctgaa gttgaccttg aaaaagtaat
    18361 agcaccatgg aaagaaatac tagcgactaa tagagagata ccaaatttag ataatcaaat
    18421 gtgtattggt ggtttagact ttgcaaacat tcgagatttt gcaagtgtag ggctattatt
    18481 ccgaaaaaac gatgattaca tttggttagg acattcgttt gtaagacaag ggtttttgga
    18541 tgatgtcaaa ttagaacctc ctattaaaga atgggaaaaa atgggattat tgaccattgt
    18601 cgatgatgat gtcattgaaa ttgaatatat agttgattgg tttttaaagg ctagagaaaa
    18661 atatgggctt gaaaaagtca tagctgataa ttatagaact gatattgtaa gacgtgcgtt
    18721 tgaggatgct ggcataaaac ttgaagtact tagaaatcca aaagcaatac atggattact
    18781 tgcaccacgt atcgatacaa tgtttgcgaa acataacgta atatatggag acaatccttt
    18841 gatgcgttgg tttactaata atgttgctgt aaaaatcaag ccggatggaa ataaagagta
    18901 tatcaaaaaa gatgaagtca gacgtaaaac ggatggattt atggcatttg ttcatgcgtt
    18961 atatagagcg gatgatatag tagacaaaga catgtctaaa gcacttgatg cattaatgag
    19021 tatagatttc taatagagga ggtgagacat gagtattcta gaaaagatat ttaaaactag
    19081 gaaagatata tcatatatgc ttgatttaga tatgatagaa gatttatcac aacaagcgta
    19141 tgtgaaacgt ttagcgattg atagttgtat tgaatttgtt gcgcgagctg tcgctcaaag
    19201 tcattttaaa gtattggaag gtaatagaat tcaaaagaat gatgtttact acaagttaaa
    19261 tataaaacca aatactgact tatcaagcga tagtttttgg caacaagtta tatataaact
    19321 aatttacgat aacgaggttt taatcgtagt aagtgacagc aaagaattac ttatcgcaga
    19381 tagcttttac agagaagagt atgctttgta tgatgatata ttcaaagatg taacggttaa
    19441 agattatact tatcaacgta ctttcacaat gcaagaggtc atatatttaa agtacaacaa
    19501 caataaagtg acacactttg tagaaagtct attcgaagat tacgggaaaa tattcggaag
    19561 aatgataggt gcacaattaa aaaactatca aataagaggg attttgaaat ctgcctctag
    19621 cgcatatgac gaaaagaata tagaaaaatt acaagcgttc acaaataaat tattcaatac
    19681 ttttaataaa aaccaactag caatcgcgcc tttgatagaa ggttttgatt atgaggaatt
    19741 atctaatggt ggtaagaata gtaacatgcc tttttctgaa ttgagtgagc taatgagaga
    19801 tgcaataaaa aatgttgcgt tgatgattgg tataccccca ggtttgattt acggagaaac
    19861 agctgatttg gaaaaaaaca cgcttgtatt tgagaagttc tgtttaacac ctttattaaa
    19921 aaagattcag aacgaattaa acgcgaaact cataactcaa agcatgtatt tgaaagatac
    19981 aagaatagaa attgtcggtg tgaataaaaa agacccactt caatatgctg aagcaattga
    20041 caaacttgta agttctggtt catttacaag gaatgaggtg cggattatgt taggtgaaga
    20101 accgtctgac aatcctgaat tagacgaata tctagtgacg aagaactacg aaaaagcaaa
    20161 cgaaaatggt agtacattga aaggtggtga tgaagatgaa agtggagatt aaaggcgtca
    20221 tcgtttccaa cgaagataaa tgggtttacg aaatgcttgg tatggattcg acttgtccta
    20281 aagatgtttt aacacaacta gaatttagtg atgaagatgt tgatattata attaactcaa
    20341 atggtggtaa cctagtagct ggtagtgaaa tatatacaca tttaagagct cataaaggca
    20401 aagtgaatgt tcgtatcaca gcaatagcag caagtgcggc atcgcttatc gcaatggctg
    20461 gtgaccacat cgaaatgagt ccggttgcta gaatgatgat tcacaatcct tcaagtattg
    20521 cgcaaggaga agcgaaagat ctaaatcatg ctgcagaaac attagaacat gttggtcaaa
    20581 taatggctga ggcatatgcg gttagagctg gtaaaaacaa acaagaactt atagaaatga
    20641 tggctaagga aacatggcta aatgctgatg aagccattga acaaggtttt gcggatagta
    20701 aaatgtttga aaacgacaat atgcaaattg tagcaagcga tacacaagtg ttatcgaaag
    20761 atgtattaaa tcgtgtaaca gctttggtaa gtaaaacgcc agaggttaac attgatattg
    20821 acgcaatagc aaataaagta attgaaaaaa taaatatgaa agaaaaggaa tcagaaatcg
    20881 atgttgcaga tagtaaatta tcagcaaatg gattttcaag attccttttt taatacaaaa
    20941 ataggaggtc ataaaatgac tataaattta tcggaaacat tcgcaaatgc gaaaaacgaa
    21001 tttattaatg cagtaaacaa cggtgaaccg caagaaagac aaaatgaatt gtacggtgac
    21061 atgattaacc aactatttga agaaactaaa ttacaagcaa aagcagaagc tgaaagagtt
    21121 tctagtttac ctaaatcagc acaaactttg agtgcaaacc aaagaaattt ctttatggat
    21181 atcaataaga gtgttggata taaagaagaa aaacttttac cagaagaaac aattgataga
    21241 atcttcgaag atttaacaac gaatcatcca ttattagctg acttaggtat taaaaatgct
    21301 ggtttgcgtt tgaagttctt aaaatccgaa acttctggcg tggctgtttg gggtaaaatc
    21361 tatggtgaaa ttaaaggtca attagatgct gcgttcagtg aagaaacagc aattcaaaat
    21421 aaattgacag cgtttgttgt tttaccaaaa gatttaaatg attttggtcc tgcgtggatt
    21481 gaaagatttg ttcgtgttca aatcgaagaa gcatttgcag tggcgcttga aactgcgttc
    21541 ttaaaaggta ctggtaaaga ccaaccgatt ggcttaaacc gtcaagtaca aaaaggtgta
    21601 tcggtaactg atggtgctta tccagagaaa gaagaacaag gtacgcttac atttgctaat
    21661 ccgcgcgcta cggttaatga attgacgcaa gtgtttaaat accactcaac taacgagaaa
    21721 ggtaaatcag tagcggttaa aggtaatgta acaatggttg ttaatccgtc cgatgctttt
    21781 gaggttcaag cacagtatac acatttaaat gcaaatggcg tatatgttac tgctttacca
    21841 tttaatttga atgttattga gtctacagtt caagaagcag gtaaggtttt aacgtacgtt
    21901 aaaggtctat atgatggtta tttagctggt ggtattaatg ttcagaaatt taaagaaaca
    21961 cttgcgttag atgatatgga tttatacact gcaaaacaat ttgcttacgg caaagcgaaa
    22021 gataataaag ttgctgctgt ttggaaatta gatttaaaag gacataaacc agctttagaa
    22081 gataccgaag aaacactata aaattttatg aggtgataaa atggtgaaat ttaaagttgt
    22141 tagagaattt aaagacatag agcacaatca acacaagtac aaagtagggg agttgtatcc
    22201 agctgaaggg tataacaatc ctcgtgttga attgttgaca aatcaaatca aaaataagta
    22261 cgacaaagtt tatatcgtac ctttagataa gctgacaaaa caagaattat tagaactatg
    22321 cgaatcatta caaaaaaaag cgtctagttc aatggttaaa agtgaaatca tcgacttatt
    22381 gaatggtgaa gacaatgacg attgatgatt tgcttgtcaa atttaaatca cttgaaaaga
    22441 ttgaccataa ttcagaggat gagtacttaa agcagttgtt aaaaatgtcg tacgagcgta
    22501 taaaaaatca gtgcggagtt tttgaattag agaatttaat aggtcaagaa ttgatactta
    22561 tacgcgctag atatgcttat caagatttat tagaacactt caacgacaat tacagacctg
    22621 aaataataga tttttcgtta tctctaatgg aggtatcaga agatgaagaa agtgtttaag
    22681 aaacctagaa ttacaactaa acgtttaaat acgcgtgttc atttttataa gtatactgaa
    22741 aataatggtc cagaagctgg agaaaaagaa gaaaaattat tatatagctg ttgggcgagt
    22801 attgatggtg tctggttacg tgaattagaa caagctatct caaacggaac gcaaaatgac
    22861 attaaattgt atattcgtga tccgcaaggt gattatttac ccagtgaaga acattatctt
    22921 gaaattgaat caagatattt caaaaatcgt ttgaatataa agcaagtatc accagatttg
    22981 gataataaag actttattat gattcgtgga ggatatagtt catgagtgtg aaagtgacag
    23041 gtgataaagc attagaaaga gaattagaaa aacattttgg cataaaagag atggtaaaag
    23101 ttcaagataa ggcgttaata gctggtgcta aggtaattgt tgaagaaata aaaaaacaac
    23161 tcaaaccttc agaagactca ggagcgctga ttagtgagat tggtcgtact gaacctgaat
    23221 ggataaaggg gaaacgtact gttacaatta ggtggcgcgg gccttttgaa cgatttagaa
    23281 tagtacattt aattgaaaat ggtcatgttg agaaaaagtc aggaaaattt gtaaaaccta
    23341 aagctatggg tgggattaat agagcaataa gacaaggtca aaataagtat tttgaaacgt
    23401 taaaaaggga gttgaaaaaa ttgtgattga tattttgtac aaagttcatg aagtgattag
    23461 tcaagacaga attattagag agcacgtaaa tatcaataat attaagttca ataaataccc
    23521 taatgtaaaa gatactgatg taccttttat tgttattgac gatatcgacg acccaatacc
    23581 tacaacttat actgacggag atgagtgtgc atatagttat attgtccaaa tagatgtttt
    23641 tgttaagtac aatgatgaat ataatgcgag aatcataaga aataagatat ctaatcgtat
    23701 tcaaaagtta ttatggtctg aactaaaaat gggaaatgtt tcaaatggaa aaccggaata
    23761 tatagaagaa tttaaaacat atagaagctc tcgcgtttac gagggcattt tttataagga
    23821 ggaaaattaa atggcaataa aacatgcaag tgcgccaaag gcgtatttta acattactgg
    23881 tttaggtttc gctaaattaa cgaaagaagg cgcggaatta aaatatagtg atattacaaa
    23941 aacaagagga ttacaaaaaa ttggtgttga aactggtgga gaactaaaaa cagcttatgc
    24001 tgatggcggt ccaattgaat cagggaatac agacggagaa ggtaaaattt cattacaaat
    24061 gcatgcgttc cctaaagaga ttcgcaaaat tgtttttaat gaagattatg atgaagatgg
    24121 cgtttacgaa gagaaacaag gtaaacaaaa caattacgta gctgtatggt tcagacaaga
    24181 gcgtagagac ggtacattta gaacagtttt attacctaaa gttatgttta caaatcctaa
    24241 aattgatggt gaaacagctg agaaagactg ggacttctca agtgaagaag tagaaggtga
    24301 ggcacttttc cctttaattg ataataaaaa gtctgtacgt aaatatatct ttgactcagc
    24361 taacatgaca aatcatggtg gcgacggtga aaaaggcgaa gaggctttct taaagaaaat
    24421 tttaggcgaa gaatatactg gaaacgtgac agaggatacg gaaactttgt aacgaaaccg
    24481 gcttcatcgg aaactgcggt aaagtcggtt agtataccag atagcattaa aacacttaaa
    24541 gttggcaaca catacgattt aaatgttgta gtagaaccat ctaatcaaag gaagctagta
    24601 aaatacacaa cagatcaaac taatgttgta tcaataaata gtgatggtca agtcactgca
    24661 gaagcgcaag gcattgccac ggttaaagca actgttggta atatgagtga cactataaca
    24721 ataaatgtag aagcataaga ggggggcaac ccctctattt tatttgaaaa taaggagagt
    24781 attataaaat ggcaaaatta aaacgtaaca ttattcaatt agtagaagac ccaaaagcaa
    24841 atgaaattaa attacaaacg tacttaacac cacacttcat ttcatttgaa attgtatacg
    24901 aagcaatgga tttaatcgat gatattgagg acgaaaatag cacgatgaaa ccaagagaaa
    24961 tcgctgacag attgatggat atggttgtaa aaatttacga taaccaattc acagttaaag
    25021 acctaaaaga acgtatgcat gcacctgatg gaatgaatgc acttcgtgaa caagtaattt
    25081 tcattactca aggtcagcaa actgaggaaa ctagaaattt tatccagaac atgaaataaa
    25141 gcctgaagat ttaacatata aagcaatgtt gaaaaatatg gatactctca tgatggactt
    25201 aattgaaaat ggtaaagacg ctaacgaagt tttaaaaatg ccatttcatt atgtactttc
    25261 catatatcaa aataaaaaca atgacatttc tgaagaaaaa gcagaggctt taattgatgc
    25321 gttttaacct taaccgtttg gttagggtta tttttttgaa cttttttaga aaggaggtaa
    25381 aaaatgggag aaagaatcaa aggtttatct ataggtttag atttggattc agcaaattta
    25441 aatagatcac ttacagaaat taaacgaaac tttagaacct taaattcaga cttaaagttg
    25501 actggaaaca actttaaata tactgagaaa tcaactgata gttataaaca acgaatcaaa
    25561 gagttagatg gaacaattgc aggttataag aaaaacgttg atgatttagc caagcaatat
    25621 gacaaggtat ctcaagaaca gggcgaaaac agtgcagaag ctcaaaagtt acgacaagaa
    25681 tataacaaac aagcaaatga gctgaattat ttagaaagag aattacaaaa aacatcagcc
    25741 gaatttgaag agttcaaaaa agctcaagtt gaagctcaaa gaatggcaga aagtggctgg
    25801 ggaaaaacca gtaaagtttt tgaaagtatg ggacctaaat taacaaaaat gggtgatggt
    25861 ttaaaatcca ttggtaaagg tttgatgatt ggtgtaactg cacctgtttt aggtattgca
    25921 gcagcatcag gaaaagcttt tgcagaagtt gataaaggtt tagatacagt tacccaagca
    25981 acaggagcaa ccggcggaga gcttaagaag ttgcagaatt catttaaaga tgtttatggc
    26041 aactttccag cagacgctga gactgtaggc ggtgttttag gggaagttaa cacaaggtta
    26101 ggtttcactg acaaagaact tgagagtgcc acagagtcat tcttgaaatt tagtcacata
    26161 acaggttctg acggcgtaca agccgttcaa ttaattacgc gtgcaatggg tgatgcaggt
    26221 attgaagctg atgagtatca aagtgtactt gatatggtag cgaaagcagc acaggctagc
    26281 ggtataagtg ttgatacatt agctgatagc attactaaat acggtgctcc aatgagggct
    26341 atgggctttg agatgaaaga atcaatcgct ttattctctc aatgggagaa atcaggtgtt
    26401 aatactgaaa tagccttcag tggtttgaaa aaagctatat ccaattgggg taaagcgggt
    26461 aaagacccaa gagaagaatt taagaagaca ttagcagaaa ttgaaaggac accggatata
    26521 gctagcgcaa caagtttagc gattgaagca tttggtgcaa aagcaggtcc tgatttagca
    26581 gatgctatta aaggcggtcg ctttagttac caagagttct taaaaactat cgaagattcg
    26641 caaggaacgg tcaatcaaac atttaaagat tctgaaagtg gctccgaaag atttaaagta
    26701 gcaatgaata aacttaaatt agtaggtgct gatgtatggg cttctattga aagtgcgttt
    26761 gctccagtaa tggaagaact aatcaaaaag ctatctatag cggttgattg gttttcaagt
    26821 ttaagtgatg gatctaaaag gtcgattgtt atattcggtg gtattgctgc tgcaattggt
    26881 cctgtagttt ttggattagg tgcattcata agcacagttg gcaacgcagt aactgtatta
    26941 gctccattat tagctagtat tgcaaaggct gacggattga ttagtttttt atcaactaaa
    27001 gtacctattt taggaacagt cttcacagca ttaactggtc caattggtat cgtgttaggt
    27061 gtactggctg gtttagcagt cgcatttaca atagcttata agaaatctga aacattcaga
    27121 aattttgtta atggtgcaat taacagtgtt aaacaaacgt ttagtaattt cattcaattt
    27181 atccaacctt tcattgattc cgttaaaaac atctttaaac aagcggtttc agcaatcgtt
    27241 gatttcgcta aagatatttg gagtcaaatt aatggattct ttaatgaaaa cggaatttct
    27301 attgttcaag cgcttcaaaa tatatgcaac tttattaaag cgatatttga atttatttta
    27361 aattttgtaa ttaaaccaat catgtttgcg atttggcaag tgatgcaatt tatttggcca
    27421 gcggttaaag ccttgattgt cagtacttgg gagaatataa aaggagtaat acaaggtgct
    27481 ttaaatatca tacttggctt tattaagttc ttttcaagtt tattcactgg taactggcga
    27541 ggcgtttggg acggtattgt gatgatacta aaaggcactg tgcagttaat ttggaattta
    27601 atacaactgt ggtttgtagg taagattcta ggtgttgtta gatactttgg tggattgctt
    27661 aaaggtttaa tatccggtat ctggggtgtt atcaaaggta ttttcacaaa atcattatct
    27721 gcaatttgga atgcaacgaa aagtattttc ggtttcttat acaatagtgt taaatctatt
    27781 ttcactaata tgaaaaactg gttatctagt acgtggaata atatcaaaag caataccgtc
    27841 ggcaaggctc attcgttatt tacgggtgta aggtctaaat tcacaagttt atggaatgcg
    27901 acgaaagata tatttactaa gttaagaaat tggatgtcaa acatctggaa ctctattaaa
    27961 gataacacgg taggtatagc tggtcgtttg tgggataaag tacgtaatat cttcggaaac
    28021 atgcgtgacg gtttaaaatc tatcattggt aaaattaaag atcatatcgg cggtatggta
    28081 agcgctatta aaaaaggact taataagctg attgaaggtt taaactgggt cggtggtaag
    28141 ttgggcatgg ataaaatacc taagttacac actggtacag agcacacaca tactactaca
    28201 agattagtta agaacggtaa gattgcacgt gacacattcg ctacagttgg ggacaaggga
    28261 cgcggaaatg gtccaaatgg ttttagaaat gaaatgattg aattccctaa tggtaaacgt
    28321 gtaatcacac caaatacaga tactaccgct tatttaccta aaggctcaaa agtatacaac
    28381 ggggcacaaa cttattcaat gttaaacgga acgcttccaa gatttagttt aggtactatg
    28441 tggaaagata ttaaatccgg tgcatcatcg gcatttaatt ggacaaaaga tcaaataggt
    28501 aaaggtacca aatggcttgg cgataaagtt ggcgatgttt tagattttat ggaaaatcca
    28561 ggcaaacttt taaattatat acttgaagct tttggaattg atttcaattc tttaactaaa
    28621 ggtatgggaa ttgcaggcga cataacaaaa gctgcatggt ctaagattaa gaaaagtgct
    28681 actgattgga taaaagaaaa tttagaagct atgggcggtg gcgatttagt cggtggaata
    28741 ttagaccctg acaaaattaa atatcattat ggacgtaccg cagcttatac cgctgaaact
    28801 ggaagaccat ttcatgaagg tgtcgatttt ccatttgtat atcaagaagt tagaacgccg
    28861 atgggtggca gacttacaag aatgccattt atgtctggtg gttatggtaa ttatgtaaaa
    28921 attactagtg gagttatcga tatgctattt gcgcatttga aaaactttag caaatcacca
    28981 cctagtggca cgatggtaaa gcccggtgat gttgttggtt taactggtaa taccggattt
    29041 agtacaggac cacatttaca ttttgaaatg aggagaaatg gacgacattt tgaccctgaa
    29101 ccatatttaa ggaatgccaa gaaaaaaggt aggttatcaa ttggtggcgg tggcgctact
    29161 tctggaagtg gtgcaactta tgccagccga gtaatccgac aagcacaaag tattttagga
    29221 ggacgttata aaggtaagtg gattcatgac cagatgatgc gagttgcaaa gcgcgaaagt
    29281 aactaccagt caaatgcagt gaataactgg gatataaatg ctcaaagagg agacccatca
    29341 agaggattat tccaaatcat cggctcaact tttagagcaa acgctaaacg tggatatact
    29401 aactttaata atccggttca tcaaggtatt tcagcaatgc agtacattgt tagacgctac
    29461 ggttgggatg gatttaagcg tgcgggtgat tacgcatatg ctacaggcgg aaaagttttt
    29521 gatggttggt ataacttagg tgaagacggt catccagaat gggttattcc aacagatcca
    29581 gctcgtagaa atgatgcaat gaagatgtta cattatgcag cagcagaagt aagagggaga
    29641 aaagcgagta aaaataagcg tcctagtcaa ttgtctagtg taaatgggtt tgatgatcca
    29701 agcttattat tgaaaatgat tgaacaacag caacaacaaa tagctttatt actgaaaata
    29761 gcgcaatcca acgatgtgat tgcagataaa gattatcagc cgattattga cgaatacgct
    29821 tttgataaaa aggtgaacgc gtctatagaa aagcgagaaa ggcaagaatc aacaaaagta
    29881 aagtttagaa aaggaggaat tgctattcaa tgatagacac tattaaagtg aacaacaaaa
    29941 caattccttg gttgtatgtc gaaagagggt ttgaaatacc ctcttttaat tatgttttaa
    30001 aaacagaaaa tgtagatgga cgttcggggg ctatatataa agggcgtagg cttgaatctt
    30061 atagttttga tataccttta gtggtacgta atgactattt atctcacaac ggcattaaaa
    30121 catatgatga cgtcttgaat gaattagtaa agttttttaa ctacgaggaa caagttaaat
    30181 tacaattcaa atctaaagat tggtactgga acgcttattt tgaaggacca ataaagctgc
    30241 acaaagaatt tacaatacct gttaagttca ctatcaaagt agtactaaca gacccttaca
    30301 aatattcagt aacaggaaat aaaaatactg cgatttcaga ccaagtttca gttgtaaata
    30361 gtgggactgc tgacactcct ttaattgttg aagcccgagc aattaaacca tctagttact
    30421 ttatgatcac taaaaatgat gaagattatt ttatggttgg tgatgatgag gtaaccaaag
    30481 aagttaagga ttacatgcct cctgtttatc atagtgagtt tcgtgatttc aaaggttgga
    30541 ctaagatgat tactgaagat attccaagta atgacttagg tggtaaggtc ggcggtgact
    30601 ttgtgatatc caatcttggc gaaggatata aagcaactaa ttttcctgat gcaaaaggtt
    30661 gggttggtgc tggcacaaaa cgagggctcc ctaaagcgat gacagatttt caaattacct
    30721 ataaatgtat tgttgaacaa aaaggtaaag gtgccggaag aacagcacaa catatttatg
    30781 atagtgatgg taagttactt gcttctattg gttatgaaaa taaatatcat gatagaaaaa
    30841 taggacatat tgttgttacg ttgtataacc aaaaaggaga ccccaaaaag atatacgact
    30901 atcagaataa accgataatg tataacttgg acagaatcgt tgtttatatg cggctcagaa
    30961 gagtaggtaa taaattttct attaaaactt ggaaatttga tcacattaaa gacccagata
    31021 gacgtaaacc tattgatatg gatgagaaag agtggataga tggcggtaag ttttatcagc
    31081 gtccagcttc tatcatagct atctatagtg cgaagtataa cggttataag tggatggaga
    31141 tgaatggatt aggttcattc aatacggaga ttctaccgaa accgaaaggc gcaagggatg
    31201 tcattataca aaaaggtgat ttagtgaaaa tagatatgca agcaaaaagt gttgtcatca
    31261 atgaggaacc aatgttgagc gagaaatcgt ttggaagtaa ttatttcaat gttgattctg
    31321 ggtacagtga attaatcata caacctgaaa acatctttga tacgacggtt aaatggcaag
    31381 atagatattt atagaaagga gatgagagtg tgatacatgt tttagatttt aacgacaaga
    31441 ttatagattt cctttctact gatgaccctt ccttagttag agcgattcat aaacgtaatg
    31501 ttaatgacaa ttcagaaatg cttgaactgc tcatatcatc agaaagagct gaaaagttcc
    31561 gtgaacgaca tcgtgttatt ataagggatt caaacaaaca atggcgtgaa tttattatta
    31621 actgggttca agatacgatg gacggctaca cagagataga atgtatagcg tcttatcttg
    31681 ctgatataac aacagctaaa ccgtatgcac caggaaaatt tgagaaaaag acaacttcag
    31741 aagcattgaa agatgtgttg agcgatacag gttgggaagt ttctgaacaa accgaatacg
    31801 atggtttacg tactacgtca tggacttctt atcaaactag atatgaagtt ttaaagcaat
    31861 tatgtacaac ctataaaatg gttttagatt tttatattga gcttagctct aataccgtca
    31921 aaggtagata tgtagtactc aaaaagaaaa acagcttatt caaaggtaaa gaaattgaat
    31981 atggtaaaga tttagtcggg ttaactagga agattgatat gtcagaaatc aaaacagcat
    32041 taattgctgt gggacctgaa aatgacaaag ggaagcgttt agagctagtt gtgacagatg
    32101 acgaagcgca aagtcaattc aacctaccta tgcgctatat ttgggggata tatgaaccac
    32161 aatcagatga tcaaaatatg aatgaaacac gattaagttc tttagccaaa acagagttaa
    32221 ataaacgtaa gtcggcagtt atgtcatatg agattacttc tactgatttg gaagttacgt
    32281 atccgcacga gattatatca attggcgata cagtcagagt aaaacataga gattttaacc
    32341 cgccattgta tgtagaggca gaagttattg ctgaagaata taacataatt tcagaaaata
    32401 gcacatatac attcggtcaa cctaaagagt tcaaagaatc agaattacga gaagagttta
    32461 acaagcgatt aaacctaata caccaaaaat taaacgacaa tattagcaat atcaatacta
    32521 tagtaaaaga tgttgtagat ggtgaattag aatactttga acgcaaaatt cataaaagtg
    32581 atacaccgcc agaaaatcca gtcaatgata cgctttggta tgatacaagt aaccctgatg
    32641 ttgctgtctt gcgtagatat tggaatggtc gatggattga agcaacacca aatgatgttg
    32701 aaaaattagg tggtataaca agagagaaag cgctattcag tgaattaaac aatattttta
    32761 ttaatttatc tatacaacac gctagtcttt tgtcagaagc tacagaatta ctgaatagcg
    32821 agtacttagt agataatgat ttgaaagcgg acttacaagc aagtttagac gctgtgattg
    32881 atgtttataa tcaaattaaa aataatttag aatctatgac acccgaaact gcaacgattg
    32941 gtcggttggt agatacaaaa actttatttc ttgagtatag aaagaaatta caagatgttt
    33001 atacagatgt agaagatgtc aaaatcgcca tttcagatag atttaaatta ttacagtcac
    33061 aatacactga tgaaaaatat aaagaagcgt tggaaataat agcaacaaaa tttggtttaa
    33121 cggtgaatga agatttgcag ttagtcggag aacctaatgt tgttaaatca gctattgaag
    33181 cagctagaga atccacaaaa gaacaattac gtgactatgt aaaaacatcg gactataaaa
    33241 cagacaaaga cggtattgtt gaacgtttag atactgctga agctgagaga acgactttaa
    33301 aaggtgaaat caaagataaa gttacgttaa acgaatatcg aaacggattg gaagaacaaa
    33361 aacaatatac tgatgaccag ttaagtgatt tgtccaataa tcctgagatt aaagcaagta
    33421 ttgaacaagc aaatcaagaa gcgcaagaag ctttaaaatc atacattgat gctcaagata
    33481 atcttaaaga gaaggaatcg caagcgtatg ctgatggtaa aatttcggaa gaagagcaac
    33541 gcgctataca agatgctcaa gctaaacttg aagaggcaaa acaaaacgca gaactaaagg
    33601 ctagaaacgc tgaaaagaaa gctaatgctt atacagacaa caaggtcaaa gaaagcacag
    33661 atgcacagag gagaacactg actcgctatg gttctcaaat tatacaaaat ggtaaggaaa
    33721 tcaaattaag aactactaaa gaagagttta atgcaaccaa tcgtacactt tcaaatatat
    33781 taaacgagat tgtccaaaac gttacagatg gaacaacaat cagatatgat gataacggag
    33841 tggctcaagc tttaaatgtg gggccacgtg gtattagatt aaatgctgat aaaattgata
    33901 ttaacggtaa tagagaaata aaccttctta tccaaaatat gcgagataaa gtagataaaa
    33961 ccgatattgt caacagcctt aatttatcaa gagagggtct tgatatcaat gttaatagaa
    34021 ttggaattaa aggcggtaac aataacagat atgttcaaat acagaatgat tctattgaac
    34081 taggtggtat tgtgcaacga acttggaaag gcaaacgatc aaccgatgat atattcacac
    34141 gtcttaaaga tggacatcta aggtttagaa ataataccgc aggcggttca ctttatatgt
    34201 cacattttgg tatttcaaca tatattgatg gagaaggcga agacggaggt tcatccggta
    34261 ctattcaatg gtgggataaa acttacagtg atagcggtat gaatggcata acaatcaatt
    34321 cctatggtgg tgtcgttgca ctaacgtcag ataataatcg ggttgttctg gagtcttacg
    34381 cttcatcgaa tatcaaaagc aaacaggcac cggtgtattt atatccaaac acagacaaag
    34441 tgcctggatt aaaccgattt gcattcacgc tgtctaatgc agataatgct tattcgagtg
    34501 acggttatat tatgtttggt tctgatgaga actatgatta cggtgcgggt atcaggtttt
    34561 ctaaagaaag aaataaaggt cttgttcaaa ttgttaatgg acgatatgca acaggtggag
    34621 atacaacaat cgaagcaggg tatggcaaat ttaatgtgct gaaacgacgt gatggtaata
    34681 ggtatattca tatacagagt acagacctac tgtctgtagg ttcagatgat gcaggagata
    34741 ggatagcttc taactcaatt tatagacgta cttattcggc cgcagctaat ttgcatatta
    34801 cttctgctgg cacaattggg cgttcgacat cagcgcgtaa atacaagtta tctatcgaaa
    34861 atcaatataa cgatagagat gaacaactgg aacattcaaa agctattctt aacttaccta
    34921 ttagaacgtg gtttgataaa gctgagtctg aaattttagc tagagagctg agagaagata
    34981 gaaaattatc ggaagacacc tataaacttg atagatacgt aggtttgatt gctgaagagg
    35041 tggagaattt aggattaaaa gagtttgtca cgtatgatga caaaggagaa attgaaggta
    35101 tagcgtatga tcgtctatgg attcatctta tccctgttat caaagaacaa caactaagaa
    35161 tcaagaaatt ggaggagtca aagaatgcag gataacaaac aaggattaca agctaatcct
    35221 gaatatacaa ttcattattt atcacaggaa attatgaggt taacacaaga aaacgcgatg
    35281 ttaaaagcgt atatacaaga aaataaagaa aatcaacaat gtgctgagga agagtaatct
    35341 ttagcactat ttttatacaa aaatttaagg aggtcattta attatggcaa aagaaattat
    35401 caacaataca gaaaggttta ttttagtaca aatcgacaaa gaaggtacag aacgtgtagt
    35461 atatcaagat ttcacaggaa gttttacaac ttctgaaatg gttaaccatg ctcaagattt
    35521 taaatctgaa gaaaacgcta agaaaattgc ggagacgtta aatttgttat atcaattaac
    35581 taacaaaaaa caacgtgtga aagtagttaa agaagtagtt gaaagatcag atttatctcc
    35641 agaggtaaca gttaacactg aaacagtatg aaaagctatg agttagatac tcataatctt
    35701 tattctttta gaaagcgggt gtactgaatt ggggtggttc aaaaaacacg aacatgaatg
    35761 gcgcatcaga aggttagaag agaatgataa aacaatgctc agcacactca acgaaattaa
    35821 attaggtcaa aaaacccaag agcaagttaa cattaaatta gataaaacct tagatgctat
    35881 tcaaaaagaa agagaaatag atgaaaagaa taagaaagaa aatgataaga acatacgtga
    35941 tatgaaaatg tgggtgcttg gtttagttgg gacaatattt gggtcgctaa ttatagcatt
    36001 attgcgtatg cttatgggca tataagagag gtgaataaaa tgtttaaact aatctttggt
    36061 tatagtttct ggacatgttt ttggttcggt aaatgtaaat aagttttagt cagtgcttcg
    36121 gtactgactt tttatttatt gttgtaatta tggtaatatg cagaagtgag caagttggat
    36181 agatggtggc tatctgagta taaggaggtg gtgcctatgg tggcattact gaaatcttta
    36241 gaaaggagac gcctaatgat tacaattagt accatgttgc agtttggttt attccttatt
    36301 gcattgatag gtctagtaat caagcttatt gaattaagca ataaaaaata accatcgcta
    36361 actttggctg gtttcgatgg ttaaatggtt attaatttaa tctttaatct aaaatagcca
    36421 ccgtcttttt aacgggctca ttagggtaac atgtttgcgc atgttgccct ttttctatat
    36481 ataaattaac acaccataat ataaatatca aatagacggc ttattagtcg tctttttatt
    36541 ttgggtaaaa ggagataaga atatgattaa ttggaaaatt agaatgaaac aaaaatcatt
    36601 ttgggtagcg atattgtcag ctatcttttt atttgctcaa aacatcgcaa aagctattgg
    36661 gtatgatatc caagtttata cagagcaatt aacagacggt ttaaacgcta tattaggatt
    36721 tttagtatta actggtgtga ttcaagaccc gactactaaa ggtataggtg atagccacca
    36781 agctttagaa tatgaagaac caagaagaaa atactaggag gtaaaataat gaaaacatac
    36841 agtgaagcaa gagcaaggtt acgttggtat caaggtagat atattgattt tgacggttgg
    36901 tatggttacc aatgtgcaga tttagcagtt gattacattt attggttgtt agaaattaga
    36961 atgtggggaa atgcaaaaga tgcaatcaat aacgatttta aaaacatggc aacagtatat
    37021 gaaaacacac catcgtttgt tccacaaata ggtgatgtgg ctgtatttac caaaggaata
    37081 tataaacaat acggtcatat tggtttagtg tttaatggtg gtaatacaaa ccaattttta
    37141 attttggaac agaactatga cggtaacgca aatacgcctg caaagttacg ttgggataat
    37201 tattacggct gtactcactt tattagacct aagtataaaa gtgagggctt aatgaataag
    37261 atcacaaata aagttaaacc acctgctcaa aaagcagtcg gtaaatctgc aagtaaaata
    37321 acagttggaa gtaaagcgcc ttataacctt aaatggtcaa aaggtgctta ttttaatgcg
    37381 aaaatcgacg gcttaggtgc tacttcagcc actagatacg gtgataatcg tactaactat
    37441 agattcgatg ttggacaggc tgtatacgcg cctggaacat taatatatgt gtttgaaatt
    37501 atagatggtt ggtgtcgcat ttattggaac aattataatg agtggatatg gcatgagaga
    37561 ttgattgtga aagaagtgtt ttaattctta ggttaaaatg ttaaatattt gttaattatt
    37621 ttttaatgta attttagttt cttttaatat tttattgatt tttaatattt tattgatttt
    37681 taatattttc tcaatataaa atgaagttgt tgatatttat catcttaaat aagggtgtta
    37741 gctataaaaa gagataaata aaaacaaata tattatattt ggaggaagcg ccatgctcaa
    37801 aagaggttta ttatttttaa ctgttttatt gttattattc tcattttctt caattactaa
    37861 tgaggtaagt gcatcaagtt cattcgacaa aggaaaatat aaaaaaggcg atgacgcgag
    37921 ttattttgaa ccaacaggcc cgtatttgat ggtaaatgtg actggagttg atggtaaagg
    37981 aaatgaattg ctatcccctc attatgtcga gtttcctatt aaacctggga ctacacttac
    38041 aaaagaaaaa attgaatact atgtcgaatg ggcattagat gcgacagcat ataaagagtt
    38101 tagagtagtt gaattagatc caagcgcaaa gatcgaagtc acttattatg ataagaataa
    38161 gaaaaaagaa gaaacgaagt ctttccctat aacagaaaaa ggttttgttg tcccagattt
    38221 atcagagcat attaaaaacc ctggattcaa cttaattaca aagattatta tagaaaagaa
    38281 ataaaacaaa atagttgttt attatagaaa gcaatgtctt gattgaatat gtgtagtgaa
    38341 aattatcttt catcaaattc tcattcatgc acgaatggtt cttccccacc taatcagata
    38401 ttaggtgact tatggggaga aatcagttag gatgaaaaag tggataatcc ttttttaggc
    38461 aggtacttcg gtacttgcct atttttttat gttataatct ttctagacgt attcaaggga
    38521 cgtcttttta gattgtatgt tatagctagc tttcgggcta gttttttgtt atgatgtgtt
    38581 acacatgcat caactattta catctatcct tgttcaccca agcatgtcac tgggtgtttt
    38641 ttcttatgat agagagcata gttttcatac tactccctcg tagtatatat gactttagca
    38701 ttcccgtata atagtttacg gggtgctttt tatgttataa ttaactgtat atagtaggag
    38761 tgaactatat agcctgttaa gtggcctagt aacctaacac ttatcctgca attgatatcc
    38821 tttttgccct tcactcgata catatatctc aacaacatag aaatattaca gtcgctacac
    38881 cgcatcttaa atggtgtggt tatttttatt ggaagtgtgt atcaggtatc agtaatgtta
    38941 aaacaccagc taaaaatgaa aagaattcac cagtgccagc aggttataca ctcgataaaa
    39001 acaatgtacc gtataaaaaa gagactggtt attacacagt tgccaatgtt aaaggtaata
    39061 acgtgaggga tggctattca actaattcaa gaattacagg tgtattaccc aataacgcaa
    39121 ctatcaaata tgacggcgca tattgcatta atggctatag atggattact tatattgcta
    39181 atagtggaca acgtcgttat atagcgacag gagaggtaga caaggcaggt aatagaataa
    39241 gcagttttgg taagtttagt gcagtttgat aattagatat ataaaggttt ggcaagttat
    39301 gaaatgtctg ccaaaccttt atataaaaaa gaaatatcta ccttttaatt tatgtaacta
    39361 ctattagtat gcatattcat tagtttttcc aggaccatta attacataag atgatttaga
    39421 ctctcctttt ttaaagaagt atgttttata cattttacct agtaactcaa cattttttct
    39481 atcttcagca agtggtgtat tcagatatac tgtatagtaa cctttatttt cagttaaaat
    39541 aaccattttt tcaaattgag cagaattttt tgtgcctttc tttaaataat ttctcaaacg
    39601 ttcatctaat tttcctagcg ttgtaggaag accactattt ttaaatgatt ctttataagc
    39661 tttttctttc tctaacattt tcttatttga ttctatttct tcatttgtag gaaacggttc
    39721 aaaagtaaaa gctttcgctg aatgatggtg tgtactgatt cctgccgtta aaaaacttaa
    39781 tgctaaaact gttgttgcta atttcttttt cataatgatg ttaattctcc ttaaattcta
    39841 tatttaaatt tatagttagt tttgcgaaat t
//
LOCUS       DAHHOJ010000012        39474 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.012, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000012 DAHHOJ010000000
VERSION     DAHHOJ010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 39474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39474
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.012"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            158..1111
                     /locus_tag="NBH32_000536"
     CDS             158..1111
                     /locus_tag="NBH32_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514167.1"
                     /translation="MKKTLGCLLLIMLLVVAGCSFGGNHKLSSKKSEESKQETVKKES
                     EEEKDPDLEKFEEIEKKMKGIKDAPSLDKLDPLMTEKSFTNSKGIQGWKDYKELMGKV
                     ELADYRFTKDSKGSSIKDVDAFFKGKKGIKRKVIETHDDVKQVDYWYVDPDGKKIGNS
                     NTPVFYAEIMTKYKDGKLVYASVEPGSYVIHKDDAIKYDDYSKLKKLSQLTKLDHPKP
                     VPYSVAQIKSFGVPLTSVSFMTHGSKDTKDEVLPALAYFTFSPKNYEDKSNPDPKVLN
                     LVHMDFLNASSDFGNAHFVVLSKYIKEYESNYETASDDSLK"
     gene            complement(1274..1477)
                     /locus_tag="NBH32_000537"
     CDS             complement(1274..1477)
                     /locus_tag="NBH32_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD1514168.1"
                     /translation="MQVNKVIYILLALFLGSFGIHKFYAGKNMQGFLHLIFFWTVIPH
                     ILAIISAIITVFKPADEHGNVTL"
     gene            1776..2264
                     /locus_tag="NBH32_000538"
     CDS             1776..2264
                     /locus_tag="NBH32_000538"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1514169.1"
                     /translation="MAIKKLVPASHPILTKKAQAVIKFDDSLKRLLQDLEDTMYAQEA
                     AGLCAPQINQSLQVAIIDMEMEGLLQLVNPKIISQSNETITDLEGSITLPDVYGEVTR
                     SKMIVVESYDVNGNKVELTAHEDVARMILHIIDQMNGIPFTERADRILTDKEVEAYFI
                     ND"
     gene            2257..3192
                     /locus_tag="NBH32_000539"
     CDS             2257..3192
                     /locus_tag="NBH32_000539"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCD1514170.1"
                     /translation="MTKIIFMGTPDFSTTVLEMLIAEHDVIAVVTQPDRPVGRKRVMT
                     PPPVKKVAMKYDLPVYQPEKLSGSEELEQLLQLDVDLIVTAAFGQLLPESLLALPKLG
                     AINVHASLLPKYRGGAPIHQAIIDGEQETGITIMYMVKKLDAGNIISQQAIKIEENDN
                     VGTMHDKLSVLGADLLKETLPSIIEGTNESVPQDDAQATFASNIRREDERISWNKPGR
                     QVFNQIRGLSPWPVAYTTMDDTNLKIYDAELVETNKINEPGTIIETTKKAIIVATNDN
                     EAVAIKDMQLAAKKRMLAANYLSGAQNTLVGKKLI"
     gene            3189..4496
                     /gene="rsmB"
                     /locus_tag="NBH32_000540"
     CDS             3189..4496
                     /gene="rsmB"
                     /locus_tag="NBH32_000540"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCD1514171.1"
                     /translation="MIENVRSLAFDTIQDILNEGAYSNLRINEVLSENELNAMDKALF
                     TEIVYGTVKRKYTLDFYLKPFVKTKIKAWVRQLLWMSIYQYVYLDKVPNHAIINEAVE
                     IAKERGGYHNGNVVNGILRTMMRSDLPDFNEIADPKKRMAIEYSMPKWIIDHWATHYG
                     LEETETILQSFLETTSTTVRANLTRASLDDIIEKLQDEGYDVEKDHDLPYCLHIGGQP
                     IIHSRSFKDGFVSIQDKSSMFVAHIMNVDRHDHVLDACSAPGGKACHIAEVLMPEGQV
                     DASDIHDHKIDLINFNIKKLRLTNIKAFQHDATKPYDKTYDKILVDAPCSGLGVMRHK
                     PEIKYTQSKQHIESLVDLQLEILENVKNNVKIGGEIIYSTCTIEQLENENVIYTFLKN
                     NKNFEFEPFQHPITGELVKTLQIMPQDFNSDGFFITKIKRKDN"
     gene            4499..5593
                     /gene="rlmN"
                     /locus_tag="NBH32_000541"
     CDS             4499..5593
                     /gene="rlmN"
                     /locus_tag="NBH32_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002435140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HCD1514172.1"
                     /translation="MITAEKKKKNKFLPNFDKQSIYSLRFDEMQNWLVEQGQQKFRAK
                     QIFEWLYQKRVDSIDEMTNLSKDLRQLLKDNFTVTTLTTVVKQESKDGTIKFLFELQD
                     GYTIETVLMRHDYGNSVCVTTQVGCRIGCTFCASTLGGLKRNLEAGEIVSQVLTVQKA
                     LDATEERVSQIVIMGIGEPFENYDEMMDFLRIVNDDNSLNIGARHITVSTSGIIPRIY
                     EFADEDIQINFAVSLHAAKDEVRSRLMPINRAYNVEKLIEAIQYYQEKTNRRVTFEYG
                     LFGGVNDQLEHARELAHLIKGLNCHVNLIPVNHVPERNYVKTAKNDIFKFEKELKRLG
                     INATIRREQGSDIDAACGQLRAKERQVETR"
     gene            5600..6343
                     /locus_tag="NBH32_000542"
     CDS             5600..6343
                     /locus_tag="NBH32_000542"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase Stp1"
                     /protein_id="HCD1514173.1"
                     /translation="MLEAQFFTDTGQHRDKNEDAGGIFYNQTNQQLLVLCDGMGGHKA
                     GEVASKFVTDELKSRFEAENLIEQHQAENWLRNNIKDINFQLYHYAQENAEYKGMGTT
                     CVCALVFEKSVVIANVGDSRAYVINSRQIEQITSDHSFVNHLVLTGQITPEEAFTHPQ
                     RNIITKVMGTDKRVSPDLFIKRLNFYDYLLLNSDGLTDYVKDNEIKRLLVKEGTIEDH
                     GNQLMQLALDNHSKDNVTFILAAIEGDKV"
     gene            6340..8334
                     /gene="pknB"
                     /locus_tag="NBH32_000543"
     CDS             6340..8334
                     /gene="pknB"
                     /locus_tag="NBH32_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein kinase Stk1"
                     /protein_id="HCD1514174.1"
                     /translation="MIGKIINERYKIVDKLGGGGMSTVYLAEDTILNIKVAIKAIFIP
                     PREKEETLKRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYIESH
                     GPLSVDTAINFTNQILDGIKHAHDMRIVHRDIKPQNILIDSNKTLKIFDFGIAKALSE
                     TSLTQTNHVLGTVQYFSPEQAKGEATDECTDIYSIGIVLYEMLVGEPPFNGETAVSIA
                     IKHIQDSVPNVTTDVRKDIPQSLSNVILRATEKDKANRYKTIQEMKDDLSSVLHENRA
                     NEDVYELDKMKTIAVPLKKEDLAKHISEHKSNQPKRETTQVPIVNGPAHHQQFQKPEG
                     TVYEPKPKKKSTRKIVLLSLIFSLLMIALVSFVAMAMFGNKYEETPDVIGKSVKEAEQ
                     IFNKNNLKLGKISRSYSDKYPENEIIKTTPNTGERVERGDSVDVVISKGPEKVKMPNV
                     IGLPKEEALQKLKSLGLKDVTIEKVYNNQAPKEYIANQSVTANTEIAIHDSNIKLYES
                     LGIKQVYVEDFEHKSFSKAKKALEEKGFKVESKEEYSDDIDEGDVISQSPKGKSVDEG
                     STISFVVSKGKKSDSSDVKTTTESVDVPYTGKNDKSQKVKVYIKDKDNDGSTEKGSFD
                     ITSDQRIDIPLRIEKGKTASYIVKVDGKTVAEKEVSYDEV"
     gene            8561..9436
                     /gene="rsgA"
                     /locus_tag="NBH32_000544"
     CDS             8561..9436
                     /gene="rsgA"
                     /locus_tag="NBH32_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HCD1514175.1"
                     /translation="MKTGRIVKSISGVYQVDVNGERFNTKPRGLFRKKKFSPVVGDIV
                     EFDVQNINEGYIHQVYERKNELKRPPVSNIDTLVIVMSAVEPNFSTQLLDRFLVIAHS
                     YQLDARVLVTKKDKTPIEKQLEINELLKIYENIGYETEFIGNDDDRKKIVEAWPAGLI
                     VLSGQSGVGKSTFLNHYRPELNLETNDISKSLNRGKHTTRHVELFERQNGYIADTPGF
                     SALDFDHIDKDEIKDYFLELNRYGETCKFRNCNHIKEPNCNVKHQLEIGNIAQFRYDH
                     YLQLFNEISNRKVRY"
     gene            9437..10081
                     /locus_tag="NBH32_000545"
     CDS             9437..10081
                     /locus_tag="NBH32_000545"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCD1514176.1"
                     /translation="MTKLYPSLLSVDFLDLQHELKRLEEAGVDGVHFDVMDGQFVPNI
                     SIGLPILDAVRKGTTLPIDVHLMIENPEKYIASFAEHGADMISIHVESTPHIHRAIQM
                     IKHLDKKAGVVINPGTPISQIEPILDIVDYVLVMTVNPGFGGQSFIDQCVEKIAGLNA
                     IKMERQLNFDIEVDGGVNTDTAKVCVENGATMLVTGSFFFKQEDYKKVTQALKG"
     gene            10088..10729
                     /locus_tag="NBH32_000546"
     CDS             10088..10729
                     /locus_tag="NBH32_000546"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HCD1514177.1"
                     /translation="MHINLLCSDRHLPQDIWAKSNEGKWGGVDRGALILLKHQIIPFF
                     SVGDFDSVSKEERQLLTEQLQIKPVQAEKADTDLALAVDKAVALGFDSITIYGATGGR
                     LDHFFGAIQLLLKKAYYKHDVHIEVIDQQNKIELLPKGQHTVEKDKSYPYISFIPMTD
                     DVELSLAGFKYNLARQMLNIGSTLTISNEIESLQAKVTVHDGLILQIRSTDLN"
     gene            complement(11089..11277)
                     /locus_tag="NBH32_000547"
     CDS             complement(11089..11277)
                     /locus_tag="NBH32_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014614133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCD1514178.1"
                     /translation="MGKQCFVTGRKASTGNRRSHALNSTKRRWNANLQKVRILVDGKP
                     KKVWVSARALKSGKVTRV"
     gene            11720..12094
                     /locus_tag="NBH32_000548"
     CDS             11720..12094
                     /locus_tag="NBH32_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1514179.1"
                     /translation="MTLEISNDYGKIDISNEVIASVVGGKAVECYGIVGMASRQQVRD
                     GIAEILGHENYAKGIKVTENNGVVNIDMYIIVSYGVKISEVANNVQSTVKYTLEKSLN
                     VSVNSINIYVQGVRVNNTGKKA"
     gene            12109..13755
                     /gene="fakA"
                     /locus_tag="NBH32_000549"
     CDS             12109..13755
                     /gene="fakA"
                     /locus_tag="NBH32_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase catalytic subunit FakA"
                     /protein_id="HCD1514180.1"
                     /translation="MISKINGKLFADMIIQGAQNLSNNADLVDSLNVYPVPDGDTGTN
                     MNLTMTSGREEVENNLSKNIGELGKTFSKGLLMGARGNSGVILSQLFRGFCKNIESES
                     EINSKLLAESFQAGVETAYKAVMKPVEGTILTVAKDAAQAAVEKANNTEDCIELMEYI
                     IVKANESLENTPNLLAVLKEVGVVDSGGKGLLCVYEGFLKALKGEKVEAKVAKIDKDE
                     FVHDEHDFHGVINTEDIIYGYCTEMMVRFGKNKKAFDEQEFRQDMSQFGDSLLVINDE
                     EIVKVHVHTEHPGKVFNYGQQYGELIKLKVENMREQHREVIRKEQHTAKPKMETVETA
                     IITISMGEGISEIFKSMGATHIISGGQTMNPSTEDIVKVIEQSKCKRAIILPNNKNIL
                     MASEQAASIVDAEAVVIPTKSIPQGISALFQYDVDATLEENKAQMADSVNNVKSGSLT
                     YAVRDTKIDGVEIKKDAFMGLIEDKIVSSQSDQLTTVTELLNEMLAEDSEILTVIIGQ
                     DAEQEVTDNMINWIEEQYPDVEVEVHEGGQPIYQYFFSVE"
     gene            13945..16005
                     /gene="recG"
                     /locus_tag="NBH32_000550"
     CDS             13945..16005
                     /gene="recG"
                     /locus_tag="NBH32_000550"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCD1514181.1"
                     /translation="MAKVNLIESPYSLLQLKGIGPKKIEALQQLNIHTVEDLVLYLPT
                     RYEDNTVIDLNQAEDQSNVTIEGQVYTAPVVAFFGRNKSKLTVHLMVNNIAVKCIFFN
                     QPYLKKKIELNQTITVKGKWNRVKQEITGNRVFFNSQGTQTQENADVQLEPVYRIKEG
                     IKQKQIRDQIRQALNDVTIHEWLTDELREKYKLETLDFTLNTLHHPKSKEDLLRARRT
                     YAFTELFLFELRMQWLNRLEKSSDEAIEIDYDLDQVKSFIDRLPFELTEAQKSSVNEI
                     FRDLKAPIRMHRLLQGDVGSGKTVVAAICMYALKTAGYQSALMVPTEILAEQHAESLM
                     ALFGDSMNVALLTGSVKGKKRKILLEQLENGTIDCLIGTHALIQDDVIFHNVGLVITD
                     EQHRFGVNQRQLLREKGAMTNVLFMTATPIPRTLAISVFGEMDVSSIKQLPKGRKPII
                     TTWAKHEQYDKVLMQMTSELKKGRQAYVICPLIESSEHLEDVQNVVALYESLQQYYGV
                     SRVGLLHGKLSADEKDEVMQKFSNHEIDVLVSTTVVEVGVNVPNATFMMIYDADRFGL
                     STLHQLRGRVGRSDQQSYCVLIASPKTETGIERMTIMTQTTDGFELSERDLEMRGPGD
                     FFGVKQSGLPDFLVANLVEDYRMLEVARDEAAELIQSGVFFENTYQHLRHFVEENLLH
                     RSFD"
     gene            16208..16780
                     /gene="fapR"
                     /locus_tag="NBH32_000551"
     CDS             16208..16780
                     /gene="fapR"
                     /locus_tag="NBH32_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription factor FapR"
                     /protein_id="HCD1514182.1"
                     /translation="MRGETLKLKKDKRREAIRQQIDSNPFITDHELSDLFQVSIQTIR
                     LDRTYLNIPELRKRIKLVAEKNYDQISSIEEQEFIGDLIQVNPNVKAQSILDITSDSV
                     FHKTGIARGHVLFAQANSLCVALIKQPTVLTHESSIQFIEKVKLNDTVRAEARVVNQT
                     AKHYYVEVKSYVKHTLVFKGNFKMFYDKRG"
     gene            16785..17771
                     /gene="plsX"
                     /locus_tag="NBH32_000552"
     CDS             16785..17771
                     /gene="plsX"
                     /locus_tag="NBH32_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCD1514183.1"
                     /translation="MVKLAIDMMGGDNAPDIVLEAVQKAVEDFKDLEIILFGDEKKYN
                     LNHERIEFRHCSEKIEMEDEPVRAIKRKKDSSMVKMAEAVKSGEADGCVSAGNTGALM
                     SAGLFIVGRIKGVARPALVVTLPTIDGKGFVFLDVGANADAKPEHLLQYAQLGDIYAQ
                     KIRGIDNPKISLLNIGTEPAKGNSLTKKSYELLNHDHSLNFVGNIEAKTLMDGDTDVV
                     VTDGYTGNMVLKNLEGTAKSIGKMLKDTIMSSTKNKLAGAILKKDLAEFAKKMDYSEY
                     GGSVLLGLEGTVVKAHGSSNAKAFYSAIRQAKIAGEQNIVQTMKETVGESNE"
     gene            17764..18690
                     /gene="fabD"
                     /locus_tag="NBH32_000553"
     CDS             17764..18690
                     /gene="fabD"
                     /locus_tag="NBH32_000553"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCD1514184.1"
                     /translation="MSKTAIIFPGQGAQKVGMAQDLFNNNDQATEILTSAANTLDFDI
                     LETMFTDEEGKLGETENTQPALLTHSSALLAALKNLNPDFTMGHSLGEYSSLVAADVL
                     SFEDAVKIVRKRGQLMAQAFPTGVGSMAAVLGLDFDKVDEICKSLSSDDKIIEPANIN
                     CPGQIVVSGHKALIEELVEKGKSLGAKRVMPLAVSGPFHSSLMKVIEEDFASYINQFE
                     WRDAKFPVVQNVNAQGETDKEVIKSNMVKQLYSPVQFINSTEWLIDQGVDHFIEIGPG
                     KVLSGLIKKINRDVKLTSIQTLEDVKGWNEND"
     gene            18683..19417
                     /gene="fabG"
                     /locus_tag="NBH32_000554"
     CDS             18683..19417
                     /gene="fabG"
                     /locus_tag="NBH32_000554"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HCD1514185.1"
                     /translation="MTKSALVTGASRGIGRSIALQLAEEGYNVAVNYAGSKEKAEAVV
                     EEIKAKGVDSFAIQANVADADEVKAMIKEVVSQFGSLDVLVNNAGITRDNLLMRMKEQ
                     EWDDVIDTNLKGVFNCIQKATPQMLRQRSGAIINLSSVVGAVGNPGQANYVATKAGVI
                     GLTKSAARELASRGITVNAVAPGFIVSDMTDALSDELKEQMLTQIPLARFGQDTDIAN
                     TVAFLASDKAKYITGQTIHVNGGMYM"
     gene            19852..20085
                     /locus_tag="NBH32_000555"
     CDS             19852..20085
                     /locus_tag="NBH32_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002510960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCD1514186.1"
                     /translation="MENFDKVKDIIVDRLGVDADKVTEDASFKDDLGADSLDIAELVM
                     ELEDEFGTEIPDEEAEKINTVGDAVKFINSLEK"
     gene            20201..20932
                     /locus_tag="NBH32_000556"
     CDS             20201..20932
                     /locus_tag="NBH32_000556"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCD1514187.1"
                     /translation="MSKQKKSEIVNRFRKRFDTKMTELGFTYQNIDLYQQAFSHSSFI
                     NDFNMNRLDHNERLEFLGDAVLELTVSRYLFDKHPNLPEGNLTKMRATIVCEPSLVIF
                     ANKIGLNEMILLGKGEEKTGGRTRPSLISDAFEAFIGALYLDQGLDIVWKFAEKVIFP
                     HVEQNELLGVVDFKTQFQEYVHQQNKGDVTYNLIKEEGPAHHRLFTSEVILQGEAIAE
                     GKGKTKKESEQRAAESAYKQLKQIK"
     gene            21079..24645
                     /gene="smc"
                     /locus_tag="NBH32_000557"
     CDS             21079..24645
                     /gene="smc"
                     /locus_tag="NBH32_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HCD1514188.1"
                     /translation="MVYLKSIDAIGFKSFADQTNVQFDKGVTAIVGPNGSGKSNITDA
                     IKWVLGEQSAKSLRGSKMEDIIFSGAEHRKAQNYAEVQLRLDNHSKKLSVDENEVIVT
                     RRLYRSGESEYYINNDRARLKDIADLFLDSGLGKEAYSIISQGRVDEILNAKPIDRRQ
                     IIEESAGVLKYKKRKAESLNKLDQTEDNLTRVEDILYDLEGRVEPLKEEAAIAKEYKT
                     LSHQMKHSDIVVTVHDIDQYTNDNRQLDQRLNDLQGQQANKEADKQRLSQQIQQYKGK
                     RHQLDNDVESLNYQLVKATEAFEKYTGQLNVLEERKKNQSETNARYEEEQENLMELLE
                     NISNEISEAQDTYKSLKSKQKELNAVIRELEEQLYVSDEAHDEKLEEIKNEYYTLMSE
                     QSDVNNDIRFLKHTIEENEAKKSRLDSRLVEVFEQLKDIQGQIKTTKKEYQQTNKELS
                     AVDKEIKNIEKDLTDTKKAQNEYEEKLYQAYRYTEKMKTRIDSLATQEEEYTYFFNGV
                     KHILKAKNKELKGIHGAVAEIIDVPSKLTQAIETALGASLQHVIVDSEKDGRQAIQFL
                     KERNLGRATFLPLNVIQSRVVATDIKSIAKEANGFISIASEAVKVAPEYQNIIGNLLG
                     NTIIVDHLKHANELARAIKYRTRIVTLEGDIVNPGGSMTGGGARKSKSILSQKDELTT
                     MRHQLEDYLRQTESFEQQFKELKIKSDQLSELYFEKSQKHNTLKEQVHHFEMELDRLT
                     TQETQIKNDHEEFEFEKNDGYTSDKSRQTLSEKETHLESIKASLKRLEDEIERYTKLS
                     KEGKESVTKTQQTLHQKQSDLAVVKERIKTQQQTIDRLNNQNQQTKHQLKDVKEKIAF
                     FNSDEVMGEQAFQNIKDQINGQQETRTRLSDELDKLKQQRIELNEQIDAQEAKLQVCH
                     QDILAIENHYQDIKAEQSKLDVLIHHAIDHLNDEYQLTVERAKSEYTSDESIDALRKK
                     VKLMKMSIDELGPVNLNAIEQFEELNERYTFLSEQRTDLRKAKETLEQIISEMDQEVT
                     ERFKETFHAIQGHFTAVFKQLFGGGDAELQLTEADYLTAGIDIVVQPPGKKLQHLSLL
                     SGGERALTAIALLFAILKVRSAPFVILDEVEAALDEANVIRYAKYLNELSDETQFIVI
                     THRKGTMEFADRLYGVTMQESGVTKLVSVNLNTIDDVLKEEQ"
     gene            24645..25895
                     /gene="ftsY"
                     /locus_tag="NBH32_000558"
     CDS             24645..25895
                     /gene="ftsY"
                     /locus_tag="NBH32_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCD1514189.1"
                     /translation="MSFFKRLKDKFATNKENEEVKSLTEEQGQDKLEDTHSEGSTQDV
                     NDLAENAEVKKKPRKLSEADFDDDGLISIEDFEEIEAQKMGAKFKAGLEKSRQNFQEQ
                     LNNLIARYRKVDEDFFEALEEMLITADVGFNTVMTLTEELRMEAQRRNIQDTEDLREV
                     IVEKIVEIYHQEDDNSEAMNLEDGRLNVILMVGVNGVGKTTTIGKLAYRYKMEGKKVM
                     LAAGDTFRAGAIDQLKVWGERVGVDVISQSEGSDPAAVMYDAINAAKNKGVDILICDT
                     AGRLQNKTNLMQELEKVKRVINRAVPDAPHEALLCLDATTGQNALSQARNFKEVTNVT
                     GIVLTKLDGTAKGGIVLAIRNELHIPVKYVGLGEQLDDLQPFNPESYVYGLFADMIEQ
                     NEEITTVENDQIVTEEKDDNHGAK"
     gene            25882..26214
                     /locus_tag="NBH32_000559"
     CDS             25882..26214
                     /locus_tag="NBH32_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HCD1514190.1"
                     /translation="MGQNDLVKTLRMNYLFDFYQSLLTNKQRNYLELFYLEDYSLSEI
                     ADTFNVSRQAVYDNIRRTGDLVEDYEKKLELYQKFEQRREIYDEMKQHLSNPEQIQRY
                     IQQLEDLE"
     gene            26240..27607
                     /gene="ffh"
                     /locus_tag="NBH32_000560"
     CDS             26240..27607
                     /gene="ffh"
                     /locus_tag="NBH32_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002457378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCD1514191.1"
                     /translation="MAFEGLSERLQATMQKMRGKGKLTEADIKIMMREVRLALLEADV
                     NFKVVKEFIKTVSERALGSDVMQSLTPGQQVIKIVQDELTKLMGGENSSINMSNKPPT
                     VVMMVGLQGAGKTTTAGKLALLMRKKYNKKPMLVAADIYRPAAINQLQTVGKQIDIPV
                     YSEGDQVKPQQIVTNALKHAKEEHLDFVIIDTAGRLHIDEALMNELKEVKDIAKPNEI
                     MLVVDSMTGQDAVNVAESFDDQLDVTGVTLTKLDGDTRGGAALSIRSVTQKPIKFVGM
                     SEKLDGLELFHPERMASRILGMGDVLSLIEKAQQDVDQEKAKDLEKKMRESSFTLDDF
                     LEQLDQVKNLGPLDDIMKMIPGMNKMKGLDKLNMSEKQIDHIKAIIQSMTPAERNNPD
                     TLNVSRKKRIAKGSGRSLQEVNRLMKQFNDMKKMMKQFTGGGKGKKGKRNQMQNMLKG
                     MNLPF"
     gene            28042..28317
                     /gene="rpsP"
                     /locus_tag="NBH32_000561"
     CDS             28042..28317
                     /gene="rpsP"
                     /locus_tag="NBH32_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCD1514192.1"
                     /translation="MAVKIRLTRLGSKRNPFYRIVVADARSPRDGRIIEQIGTYNPTS
                     ANAPEIKVDEALALKWLNDGAKPTDTVHNILSKEGIMKKFDEQKKAK"
     gene            28505..29008
                     /gene="rimM"
                     /locus_tag="NBH32_000562"
     CDS             28505..29008
                     /gene="rimM"
                     /locus_tag="NBH32_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCD1514193.1"
                     /translation="MRVEVGQIVNTHGIKGEIKVKSNSDFTDVRFQPGQVLTVVHNNN
                     DLEYTVKSHRVHKGLHMLTFEGINNINDIEHLKGSSIYQERDHEDIVLEENEFYYSDI
                     IGCTVFDDQETPIGRVINIFETGANDVWVIKGSKEYLIPYIADVVKEVDVENKKIIIT
                     PMEGLLD"
     gene            29008..29745
                     /gene="trmD"
                     /locus_tag="NBH32_000563"
     CDS             29008..29745
                     /gene="trmD"
                     /locus_tag="NBH32_000563"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCD1514194.1"
                     /translation="MKIDYLTLFPEMFDGVLNHSIMKRAQENNKLQINTVNFRDYAIN
                     KHNQVDDYPYGGGQGMVLKPEPVFNAMEDLDVTEQTRVILMCPQGEPFSHQKAVELSK
                     ADHIVFICGHYEGYDERIRTHLVTDEISMGDYVLTGGELPAMTMTDAIVRLIPGVLGN
                     EQSHQDDSFSDGLLEFPQYTRPREFKGLTVPDVLLSGNHANIDAWRHEQKLIRTYNKR
                     PDLIEKYPLTNADKQILERYKIGLKKG"
     gene            29848..30198
                     /gene="rplS"
                     /locus_tag="NBH32_000564"
     CDS             29848..30198
                     /gene="rplS"
                     /locus_tag="NBH32_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCD1514195.1"
                     /translation="MTNHKLIEAVTKSQLRTDLPSFRPGDTLRVHVRIIEGTRERIQV
                     FEGVVIKRRGGGVSETFTVRKISSGVGVERTFPLHTPKIEKIEVKRRGKVRRAKLYYL
                     RSLRGKAARIQEIR"
     gene            complement(30439..33045)
                     /gene="yfhO"
                     /locus_tag="NBH32_000565"
     CDS             complement(30439..33045)
                     /gene="yfhO"
                     /locus_tag="NBH32_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosyltransferase
                     YfhO"
                     /protein_id="HCD1514196.1"
                     /translation="MLKKWLNSNVKQFFVITFISVILTLILFSTHIYDYIVNGTVFSG
                     AGDGFRQMMPFQMYLYEHLSSFSSLYDASFGLGGDYMKGLSYYYSLSPLMWLNFLFIK
                     IGETVGIFNPTTIHFWPTNQLIMAMIRAIITFVVTFYLFKILHFKRSTNMIATILYGM
                     STVVIYFNFTWSFYGNLLYLLPLSILGLERYFQQRKIGIFIVAIALTLFSNFYFSYYQ
                     AIIIGCYYLYRLIFTYKYDIVSRTQKLICVISAAVLSVLSSVFGLFTGISAFLENDRK
                     QNPNVDIPFLTPLDYHYFFFSDGFYITISILTIVALLSFKLYRFYFYRLFAIVTWILF
                     IGSLSQYFDSAFNGFSFPERRWVYILALSSSALCGLFIQHLSTLNMKYYLIRTIPVSI
                     IALLYVLLSPTHPLVLIVGIILLMVLAVILKFSLWRYKKLTVVLIVLIVMIQQIVILD
                     NNKNMAIKPYQQSISTLKQHDYHSNYVNQLIKKINQNATGPFNRIDYMSDYALNSPFI
                     YHYNGISLYSSIFNGDILKYYDKTLQINMPIDKNSTYRLLGNRQNLLSLWNVNDRIRV
                     NHDYNLPYGFKINSEHKDNKVRWIHSKNTIHYPSAHITNKVFSNKELKSPLDKEQAML
                     QGIVSNNTKDVNTHFKANKNLLSDSTIKLNSAAWQSPSKHLLQVKQNNGGLTVQLPKS
                     VSNQFKDLYFEMDLELLSPDKAHYVKVNEYTLERNKLTYKYRRFVTPVTIRIKSSDAI
                     KLSLPKGKYRVNLKGIYGEDYTTLKDTSNSLEAVKVSKTKQGYTITKNKNSSGYIVLP
                     TAYNQGMKATSGDQSLKVEQVNGVMTGIKAPKNITKIQLSYTPPYYYLLITITIFGII
                     CSIIFTRWVRQK"
     gene            33446..34330
                     /gene="ylqF"
                     /locus_tag="NBH32_000566"
     CDS             33446..34330
                     /gene="ylqF"
                     /locus_tag="NBH32_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HCD1514197.1"
                     /translation="MVIQWYPGHMAKAKREVSEQLKKVDVVFELVDARIPYSSRNPMI
                     DEVINQKPRVVILNKKDMSNLNEMSKWEQFFIDKGYYPVSVDAKHGKNLKKVEAAAIK
                     ATAEKFEREKAKGLKPRAIRAMIVGIPNVGKSTLINKLAKRSIAQTGNKPGVTKQQQW
                     IKVGNALQLLDTPGILWPKFEDEEVGKKLSLTGAIKDSIVHLDEVAIYGLNFLIQNDL
                     ARLKAHYKIEVPEDAEIIAWFDAIGKKRGLIRRGNEIDYEAVIELIIYDIRNAKIGNY
                     CFDIFKDMTEELANDANN"
     gene            34314..35081
                     /locus_tag="NBH32_000567"
     CDS             34314..35081
                     /locus_tag="NBH32_000567"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCD1514198.1"
                     /translation="MTLTIKEVTQLINAVNTIEELENHECFLDERKGVQNAIARRRKA
                     LEQEQALKEKYAEMTYFENEILKEHPNAIICGIDEVGRGPLAGPVVACATILNSNHNY
                     LGLDDSKKVPVTKRLELNEALKNEVTAFAYGIATAEEIDEFNIYKATQIAMQRAIDGL
                     SVQPTHLLIDAMTLDNALTQVSLIKGDARSVSIAAASIMAKVFRDDYMTQLSKDYPEY
                     GFEKNAGYGTKQHLLAIDDIGIMKEHRKSFEPIKSLL"
     gene            35190..36356
                     /gene="sucC"
                     /locus_tag="NBH32_000568"
     CDS             35190..36356
                     /gene="sucC"
                     /locus_tag="NBH32_000568"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCD1514199.1"
                     /translation="MNIHEYQGKEIFRSMGVAVPEGRVAFTAEEAVEKAKELNSDVYV
                     VKAQIHAGGRGKAGGVKIAKSLSEVETYAKELLGKTLVTHQTGPEGKEIKRLYIEEGC
                     AIQKEYYVGFVIDRATDQVTLMASEEGGTEIEEVAAKTPEKIFKETIDPVIGLSPFQA
                     RRIAFNINIPKKSVNKAAKFLLALYNVFIEKDCSIVEINPLVTTADGDVLALDAKINF
                     DDNALFRHKDVVELRDLEEEDPKEIEASKHDLSYIALDGDIGCMVNGAGLAMATMDTI
                     NHFGGNPANFLDAGGSATREKVTEAFKIILGDENVKGIFVNIFGGIMKCDVIAEGIVE
                     AVKEVDLTLPLVVRLEGTNVELGKKILKDSGLAIEPAATMAEGAQKIVKLVKEA"
     gene            36378..37286
                     /gene="sucD"
                     /locus_tag="NBH32_000569"
     CDS             36378..37286
                     /gene="sucD"
                     /locus_tag="NBH32_000569"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCD1514200.1"
                     /translation="MSVFIDKNTKVMVQGITGSTALFHTKQMLDYGTKIVAGVTPGKG
                     GQVVEGVPVFNTVEEAKNETGATVSVIYVPAPFAADSILEAADADLDMVICITEHIPV
                     LDMVKVKRYLQGRKTRLVGPNCPGVITADECKIGIMPGYIHKKGHVGVVSRSGTLTYE
                     AVHQLTEEGIGQTTAVGIGGDPVNGTNFIDVLKAFNEDDETKAVVMIGEIGGTAEEEA
                     AEWIKANMTKPVVGFIGGQTAPPGKRMGHAGAIISGGKGTAEEKIKTLNSCGVKTAAT
                     PSEIGSTLIEAAKEAGIYESLLTVNK"
     gene            37513..38631
                     /locus_tag="NBH32_000570"
     CDS             37513..38631
                     /locus_tag="NBH32_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1514201.1"
                     /translation="MNKQQSKVRYSIRKVSIGILSISIGMFLALGMSNKAYADEIDKS
                     KDFTRGYEQNVFAKSELNANKNTTKDKIKNEGVVKTSDTSLKLDNKSAISNGNEINQD
                     IKISNTPKNSSQGNNLVVNNNEPTKEIKIANLEAQNSNQKKTNKVTNNYFGYYSFREA
                     PKTQIYTVKKGDTLSAIALKYKTTVSNIQNTNNIANPNLIFIGQKLKVPMTPLVEPKP
                     NTVSSNNKSNSNSSTLNYLKTLENRGWDFDGSYGWQCFDLVNVYWNHLYGHGLKGYGA
                     KDIPYANNFNSEAKIYHNTPTFKAEPGDLVVFSGRFGGGYGHTAIVLNGDYDGKLMKF
                     QSLDQNWNNGGWRKSEVAHKVVHNYENDMIFIRPFKKA"
     gene            38659..>39474
                     /gene="fmhC"
                     /locus_tag="NBH32_000571"
     CDS             38659..>39474
                     /gene="fmhC"
                     /locus_tag="NBH32_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhC"
                     /protein_id="HCD1514202.1"
                     /translation="MKFTTLSEEEFTNYTKKHFKHYTQSIELYNYRNKINYEAHIVGV
                     KNDKNEVIAACLLTEARIFKFYKYFYSHRGPLLDYFDAKLVCYFFKELSKFIYKNRGV
                     FILVDPYLIENLRDANGRVIKNYNNSVIVKMLGKIGYLHQGYTTGYSNKSQIRWISVL
                     DLKDKDENQLLKEMEYQTRRNIKKTIEIGVKVEDLSIEETNRFYKLFQMAEEKHGFHF
                     MNEDYFKQMQEIYKGKAMLKIACIDLNEYQDKLKIQLLKIENEMMTVNRSLNEN"
BASE COUNT        14778 a         5270 c         7549 g        11877 t
ORIGIN      
        1 taactactta aattattaga caaaacaatt ttcttaaatt atcaattaaa gtatttaaaa
       61 atattgatgg ttaatgtaaa atatcaatat aaataagttt tttaaattta tgtatgttta
      121 tttgattcaa acaaaataac ttaagaggag aaagtttatg aaaaagacac tgggatgttt
      181 acttttaatt atgcttttag tcgtagcagg ttgttctttt ggtggtaatc ataaattatc
      241 atcaaagaaa tcagaagaat caaaacaaga aactgtaaaa aaagaatcgg aagaagagaa
      301 agacccagat ttagagaaat ttgaagaaat agagaagaaa atgaaaggaa ttaaagatgc
      361 gccatctcta gataagttgg atccattaat gacagaaaag tcgtttacga atagtaaagg
      421 gattcaagga tggaaagatt acaaagaatt aatgggtaaa gtggaacttg cagattatag
      481 atttacaaaa gattcaaaag gatcttcaat aaaagatgtt gatgcattct ttaaaggtaa
      541 gaaaggtata aaaaggaaag tgattgaaac acacgatgat gtaaaacaag ttgattattg
      601 gtatgtagat ccagatggaa agaaaattgg caattcaaac acacctgttt tttacgcaga
      661 aattatgaca aaatataaag atggaaagtt agtttatgca tcagtcgaac caggttctta
      721 cgtaatacat aaagatgatg caattaaata tgacgattat tctaagttaa aaaaattaag
      781 tcaactaact aaacttgatc atccaaaacc agttccatat agcgtagctc aaatcaaatc
      841 tttcggagta cctttaacaa gcgtttcatt tatgacacat ggatcaaagg atactaaaga
      901 tgaagtgttg ccggcattgg cctatttcac tttttcacca aaaaattatg aagacaagtc
      961 taatccagat ccaaaagttt taaatttagt acatatggat ttcttaaatg catctagtga
     1021 ttttggtaac gcacattttg ttgttttaag taaatatatt aaagagtatg aatcaaacta
     1081 tgaaacagcg tcagatgatt ctttaaaata gtatttactg tgtgaaaaat aaatagtgta
     1141 ctacattaaa taatcgcaat aataatcccg ataaacaatc agcattactg cttatcatat
     1201 agagttcgta atatctataa ctctatgatt cgcaattaat aaatgattgt catcgggatt
     1261 tatttttatc aatttataat gtaacattac catgttcgtc agctggtttg aaaacagtaa
     1321 ttatcgcact aataattgct aatatatgtg ggatgactgt ccaaaagaat attaagtgta
     1381 ggaaaccttg catgtttttg ccggcataaa atttatgaat accaaaacta cctaagaaca
     1441 atgctaataa aatataaata actttgttta cttgcatttc tttccctcca gttgaattgc
     1501 ttataatgac attaacttct ctttttatta tacccacttt tagttcaaac attctagttt
     1561 aagcatttcc aatcatctag atttcagtta ttcaatcctt acaataaatt taggattaca
     1621 cttcagttgc attgtattat tttacgtgtg aaatatacgt aatgaatcac atgacaatct
     1681 ttaaattgaa aaatatacat tctatgatgt aaggtcgcat ttttaatata tttacgttat
     1741 aatggtttga tgaattttat tgaggagtaa gagctatggc gattaaaaag ttagtaccag
     1801 catcgcatcc tattttaacg aaaaaagcgc aagcagttat aaaatttgat gattcgttaa
     1861 aaagattatt acaagattta gaagatacaa tgtatgcaca agaagcagct ggcttatgtg
     1921 cacctcaaat taatcagtca ttgcaagtgg caatcattga tatggaaatg gaaggattat
     1981 tacaacttgt taatccgaaa attattagtc aatcaaatga aacgataaca gacttagaag
     2041 gttcaattac attgccagat gtttacggcg aagtgacaag aagtaaaatg atcgttgtcg
     2101 aaagttatga cgtcaatggg aacaaagttg aactaactgc acatgaagat gtagcaagaa
     2161 tgattttgca tattatagat caaatgaacg gtatcccttt tacagaacgt gcggaccgta
     2221 ttttaacaga taaagaagtg gaggcatatt ttataaatga ctaaaataat atttatgggt
     2281 acaccagact tttcaacaac tgttttagaa atgcttattg cagaacatga tgtcattgca
     2341 gtcgtaacgc aaccagatcg acctgttgga cgtaaacgtg ttatgacacc accaccagtt
     2401 aaaaaagttg caatgaaata tgatttacct gtatatcagc ctgaaaaact aagtggatca
     2461 gaagaattag aacaattgct tcaattagat gtagatttaa ttgtaactgc tgcttttgga
     2521 caattattac ctgaatcatt gttggcatta ccaaaacttg gcgcaattaa tgtacatgca
     2581 tcattgttac cgaagtatag aggtggtgca ccaattcatc aggcaattat cgatggtgaa
     2641 caagaaaccg gcataacaat tatgtatatg gttaaaaaat tagatgcggg taatattatt
     2701 tcgcaacaag caattaaaat agaagaaaat gataatgtcg gtacgatgca tgataaatta
     2761 agtgtattag gggcagattt attaaaagaa actttaccat ctattataga gggcacaaat
     2821 gaaagtgtac ctcaagatga tgcgcaagca acatttgctt ccaatattcg acgcgaagat
     2881 gagcgaatta gctggaataa accaggaaga caagtgttta atcaaattcg aggattatca
     2941 ccatggccag ttgcttatac aactatggat gacactaact tgaaaatata cgatgctgaa
     3001 ctcgttgaga ctaataagat aaacgagcct ggaacaatta tagaaacgac taaaaaagcc
     3061 attattgttg ctacaaatga taatgaagct gttgcaatta aagatatgca attggctgcg
     3121 aaaaagagaa tgttagctgc caattattta agtggtgcgc aaaacacact agtagggaag
     3181 aaacttatat gatagaaaac gtgagaagtc ttgcttttga cacgattcaa gatatattaa
     3241 atgaaggtgc gtatagtaac ttgcgtatca atgaagtgtt gtcagaaaat gaattaaatg
     3301 caatggataa agctttattt acagaaattg tctacggaac cgttaaaaga aaatatacgt
     3361 tagattttta tttaaagcct tttgtgaaaa caaagattaa ggcatgggtt aggcaattat
     3421 tatggatgag tatttatcaa tatgtttatt tagataaagt tccaaatcat gccattatta
     3481 atgaagcagt tgaaatagca aaagaacgcg gtggctatca taatggtaat gtcgtaaatg
     3541 gtattttacg tacaatgatg cgtagtgact tacctgattt taatgaaatt gcagatccta
     3601 aaaaaagaat ggcaatcgag tatagtatgc cgaagtggat tatagatcat tgggcaacac
     3661 attatggtct cgaagaaact gaaacaatct tacagtcatt tttagaaacg acatctacaa
     3721 ctgtgcgtgc caacctgacg cgagcatcat tagatgatat tattgaaaag ttgcaagacg
     3781 aaggttatga cgttgaaaaa gatcatgact taccttattg tctccatata ggaggacaac
     3841 caattattca ttctcgttca tttaaggatg gattcgtttc aattcaagat aaaagctcaa
     3901 tgtttgttgc gcacattatg aatgtagacc gacatgatca cgtattagat gcatgtagtg
     3961 cacctggcgg taaggcttgt cacattgctg aagttttaat gccagaaggg caagttgacg
     4021 cttcagatat acatgatcac aaaatagact taattaattt taatataaaa aaattacgat
     4081 taacaaatat taaagccttt caacatgatg cgacaaaacc ttatgataaa acatacgata
     4141 agatacttgt tgatgcacca tgtagcggat taggtgtaat gagacataag ccggagatta
     4201 agtatactca aagcaaacaa catattgagt cactagttga tttacagctt gaaatattgg
     4261 aaaatgtaaa aaacaatgta aaaataggtg gcgaaatcat ctattcaaca tgtacaatag
     4321 agcaactaga aaatgaaaac gtgatttata cgtttttgaa aaataataaa aacttcgaat
     4381 ttgaaccgtt tcaacatccg ataactggag agttggtcaa aacgttacaa atcatgccgc
     4441 aagactttaa ttcagatgga ttctttatca ctaagataaa aagaaaggac aattaggaat
     4501 gataactgct gaaaagaaaa agaagaataa atttcttcca aattttgaca agcaatcaat
     4561 atattcattg cgatttgacg aaatgcaaaa ctggctcgtt gaacaagggc aacaaaaatt
     4621 tcgagcgaaa cagatttttg aatggttata tcaaaaaaga gtagattcga ttgatgaaat
     4681 gacgaactta tcgaaagact tacgacagct cttaaaagat aactttactg ttacaacttt
     4741 aacaactgta gtaaaacaag aaagtaaaga cggtacaatt aaattcttat ttgaattaca
     4801 agatggctat acaattgaaa ctgttttaat gagacatgat tatggaaatt cagtatgtgt
     4861 aacaacacaa gtaggttgtc gtatcggatg tacgttttgt gcttctacac ttggcggctt
     4921 aaaaagaaac cttgaagctg gcgaaattgt ttcacaagtt ttaacagttc aaaaagccct
     4981 tgatgctaca gaagagcgcg tatctcaaat tgtcataatg ggtatcggtg aaccatttga
     5041 aaattatgat gaaatgatgg actttttaag aatcgtcaat gatgataaca gtttaaatat
     5101 tggtgcacgt cacattacag tatcaacatc aggtatcatt cctagaatat atgaatttgc
     5161 ggatgaagat attcaaatta attttgctgt aagcttacac gccgcaaaag atgaagtgcg
     5221 atcacgcttg atgccaatta accgtgcata taatgttgag aagttaatcg aagcaattca
     5281 atattatcaa gaaaaaacaa atcgtcgtgt tacttttgaa tatggtctgt ttggtggtgt
     5341 aaatgaccaa ctagaacatg caagagaatt agcacattta ataaaaggct taaattgcca
     5401 tgttaactta attcctgtca accatgttcc agaaagaaat tatgtaaaaa cggctaaaaa
     5461 tgatatcttt aaatttgaaa aagaattaaa gagactagga attaatgcca caatacgtcg
     5521 tgaacaaggt tcggatattg acgcagcttg tggtcaatta agagcaaagg aacgacaagt
     5581 agaaacgagg taaagacaaa tgctagaggc acaatttttt actgatactg gacaacatag
     5641 agataagaat gaagatgcgg gtggtatttt ttataatcaa actaatcaac aacttttagt
     5701 tctgtgtgat ggtatgggcg gccataaagc aggagaagtt gcaagtaaat ttgttacaga
     5761 tgagttgaaa tcccgttttg aagcggaaaa tcttatagaa caacatcaag ctgaaaattg
     5821 gttgcgtaat aatataaaag atataaattt tcagttatat cactatgcac aagaaaatgc
     5881 agaatataaa ggtatgggta caacatgtgt ttgtgcactt gtttttgaaa aatcagttgt
     5941 gatagcaaat gtcggtgatt ctagagccta tgttattaat agtcgacaaa ttgaacaaat
     6001 tactagtgat cactcatttg ttaatcatct tgttttaacg ggtcaaatta cgccggaaga
     6061 agcatttaca catccacaac gtaatattat tacaaaggtg atgggcacag ataaacgtgt
     6121 gagtccagat ttgtttatta agcgattaaa tttttatgat tatttattat taaattcaga
     6181 tggattaact gattatgtta aagacaatga aattaagcgt ttgttagtaa aagaaggtac
     6241 aatagaagat catggtaatc aattaatgca attggcatta gataaccatt cgaaagataa
     6301 cgttactttc atactcgcgg ctattgaagg tgataaagta tgataggtaa aataataaat
     6361 gaacgatata aaattgtaga taagcttggc ggcggtggca tgagtaccgt ttatcttgca
     6421 gaagatacga tacttaacat taaagttgca attaaggcga tttttatacc acctagagaa
     6481 aaagaagaaa cattaaaacg ttttgaacga gaagtacata actcatcaca gctatcacat
     6541 caaaatatag taagtatgat cgatgttgat gaagaagatg actgttacta cttagtaatg
     6601 gaatatattg aaggtccgac tttgtctgag tatattgaaa gtcatgggcc attaagtgtt
     6661 gacacggcga ttaattttac gaatcaaata ttggatggca ttaaacatgc gcatgatatg
     6721 cgtattgtac atagagatat taagccacaa aatatattaa ttgacagcaa taaaacgttg
     6781 aaaatatttg attttggaat tgctaaagct ttaagtgaga cgtctttaac tcagactaat
     6841 catgtgttag gtactgtgca gtacttttcg ccagaacaag caaaaggtga ggcaacggat
     6901 gaatgtacag atatttattc tataggtatt gtgttatatg aaatgcttgt tggtgagccg
     6961 ccatttaatg gagaaactgc agttagcatt gcgattaaac atattcagga ttctgttcca
     7021 aatgtgacaa cagatgtacg taaggatatt ccgcaatctt taagtaatgt cattttacgc
     7081 gctacagaaa aagacaaagc gaatcgttac aaaacaattc aagaaatgaa agatgatttg
     7141 agtagtgttt tacatgaaaa tcgagcgaat gaagatgtct atgaactcga taaaatgaaa
     7201 acgatagcgg tacctttgaa aaaagaagat ctagcaaagc atattagtga acataagtcg
     7261 aatcaaccta aacgtgaaac gacgcaagta cctattgtaa atgggcctgc tcatcatcag
     7321 caattccaaa agccagaagg tacggtgtac gaaccaaaac ctaaaaagaa atcaacacga
     7381 aagattgtgc tcttatcatt aatcttttcg ttgttaatga ttgcacttgt ttcttttgtg
     7441 gcaatggcaa tgtttggtaa taaatacgaa gagacacctg atgtaatcgg gaaatctgta
     7501 aaagaagcag agcaaatatt caataaaaac aacctgaaat tgggtaaaat ttctagaagt
     7561 tatagtgata aatatcctga aaatgaaatt attaagacaa ctcccaatac aggtgaacgt
     7621 gttgaacgtg gtgacagtgt tgatgttgtt atatcaaaag gccctgaaaa ggttaaaatg
     7681 ccaaatgtca ttggtttacc taaggaggaa gccttgcaga aattaaaatc gttaggtctt
     7741 aaagatgtta cgattgaaaa agtatataat aatcaagcgc caaaagaata cattgcaaat
     7801 caaagtgtaa ccgcaaatac tgaaatcgct attcatgatt ctaatattaa actatatgaa
     7861 tctttaggca ttaagcaagt ttatgtagaa gactttgaac ataaatcctt tagcaaagct
     7921 aaaaaagcct tagaagaaaa agggtttaaa gttgaaagta aggaagagta tagtgacgat
     7981 attgatgagg gtgatgtgat ttctcaatct cctaaaggaa aatcagtaga tgaggggtca
     8041 acgatttcat ttgttgtttc taaaggtaaa aaaagcgact catcagatgt caaaacgaca
     8101 actgaatcgg tagatgtacc atacactggt aaaaatgata agtcacaaaa agttaaagtt
     8161 tatattaaag ataaagataa tgacggttca actgaaaaag gtagtttcga cattactagt
     8221 gatcaacgta tagacattcc tttaagaatt gaaaaaggaa aaacagcaag ttatattgtt
     8281 aaagttgacg gtaaaactgt agctgaaaaa gaagtcagct atgatgaagt ttaaatataa
     8341 ttgtaataag tgtaccgagg tttctatttg gaagtctcgg tattttttgt tgagattgcg
     8401 gtagttttaa aatgctttat gacatcatat acaggtgagc attgtctatc aattctgttt
     8461 cactcccaca atatttatat catttgaaat gatcatttat atattaattt tccagtcttt
     8521 gatataataa atgcagtaaa attaatcgag aggtgccatt ttgaagacag gtcgaatagt
     8581 gaaatcaatt agtggggtat atcaagtaga cgttaatggc gaacgtttca atacaaaacc
     8641 acgaggatta tttagaaaga aaaaattttc accagtagtt ggagatatag tggaatttga
     8701 tgtacaaaac attaatgaag gctatattca tcaagtgtat gagcgaaaaa atgagttgaa
     8761 aagaccacct gtaagtaata tagatacact agtaattgta atgagtgctg tcgagccaaa
     8821 tttttcaacg caattattag atcgattttt agttattgca cattcgtatc agttagatgc
     8881 gagagttttg gtgactaaaa aagataaaac accaattgaa aagcagcttg aaattaatga
     8941 gttgttgaaa atatatgaaa atattggcta tgagactgaa tttattggaa atgatgatga
     9001 tcgaaaaaaa attgtagaag cttggccagc aggacttata gtacttagtg gtcaatcagg
     9061 tgtcggtaag tccactttct taaatcatta tcgtccagaa cttaatcttg agacaaatga
     9121 tatatcaaaa tcattaaatc gaggaaagca tactacaaga catgtcgaac tattcgaacg
     9181 tcaaaacggt tatattgcag acacacctgg attcagtgct ttagattttg atcatataga
     9241 taaagatgaa ataaaagatt attttcttga attaaatcga tatggtgaaa catgtaagtt
     9301 tagaaattgt aatcatatca aagaacctaa ttgtaatgtt aagcatcaat tagagatagg
     9361 gaatattgcg caatttagat acgaccatta tttacaacta tttaatgaaa tttcaaatag
     9421 aaaggttaga tattaaatga caaaactata tccatcatta ttatctgttg attttttgga
     9481 tttacaacat gaattaaaac gacttgaaga agcaggtgtc gacggagttc attttgatgt
     9541 tatggatggt caatttgtgc ctaatatatc tattggttta ccaatattag atgcagtgag
     9601 aaaaggcaca acattaccta tagacgtaca tttgatgatt gaaaatccag aaaagtatat
     9661 tgcatcattt gcagaacatg gtgccgatat gatttcaatt catgtcgaat caacgcctca
     9721 tatccatcgt gctattcaaa tgattaaaca tttagataaa aaagctggtg tagtaattaa
     9781 tcctggtaca ccaatatcac aaattgaacc tattttagac attgttgatt atgtactagt
     9841 gatgacagtt aacccagggt ttggtggtca atcatttatt gatcaatgcg tagaaaaaat
     9901 agcgggtctt aatgctatta aaatggaacg tcaattaaac tttgatattg aagttgatgg
     9961 aggcgtaaat accgatacag cgaaagtttg tgttgaaaat ggtgctacaa tgttagtaac
    10021 aggttcattt ttctttaaac aagaggatta taaaaaagtc acacaagcat tgaaaggttg
    10081 agtgtatatg catataaatt tattatgttc tgatcgacac ttgccgcaag atatttgggc
    10141 caaaagtaat gaaggtaaat ggggcggcgt tgatagaggt gctttgattt tattgaagca
    10201 tcaaattatc ccttttttct cagtgggaga ctttgattca gtcagtaaag aagaacgcca
    10261 acttctaaca gaacagttac aaatcaaacc agttcaagct gaaaaagctg atacggattt
    10321 agctttagcg gttgataaag ctgttgcact tgggtttgat agtattacaa tttatggtgc
    10381 aacaggcgga cgattagatc acttttttgg ggcaattcag ttattattga aaaaagcata
    10441 ttataaacat gatgttcata tagaagttat cgatcaacaa aataaaattg aattattacc
    10501 taaaggtcaa catacagttg aaaaagataa gagttatccg tacatttcat ttataccgat
    10561 gactgatgat gtagaacttt ctctagcagg ttttaaatat aatttagcta gacaaatgct
    10621 taatataggt tctactttaa ctatttcaaa tgaaattgag tctttgcaag cgaaagtaac
    10681 tgtacatgat gggttgattt tgcaaattag aagtacagat ttaaattaag tatattcaaa
    10741 tttgtgagga tttttaataa agtgacgttt atagatagaa tttataacat aataatagat
    10801 gaatcacgct gtaattaatg aagattatgg cgtgatttat tatcgaaagg aaaattgtag
    10861 gtcctagtac attgatttat gtgccaaaat cgatattgaa cccataagtc caatttgaca
    10921 gagaaccatt attaaacgta ttaaccaaat tatattgata gttcgaattt cactatgaac
    10981 cattatgtat tgtttggtaa caaaaaaaga actaagaggt tgagctctta gttcttaagt
    11041 aaatgtgtcc tccacataag gtcacatttt ttatgtgcaa tatatttatt aaactctagt
    11101 tactttacca gattttaaag cacgtgcaga aacccaaact tttttaggtt taccgtcaac
    11161 taggattcta actttttgaa ggttagcgtt ccatctacgt ttagtagagt ttaaagcgtg
    11221 tgaacgtctg ttaccagtcg aagctttacg acctgttacg aaacattgtt tacccatatg
    11281 agtacctcct ttaataaata taaatacaca taactactta tatacttaac taagatagca
    11341 tagtttcatt tgaaaaacaa tgaataattt tcacataaaa gtcaaaaata ctgagttttg
    11401 tgatataatt gtagactgtg aagttatgta gtatgatatt ttagagaaat aagtgaatga
    11461 aacatttaaa ctttttattt aatgatacta catctattaa aagaaaacgc ctatagataa
    11521 tcgtcatgag tgtaaattca gtttactcaa aatataatag gtgatttaag cgtaagtcat
    11581 tctgataagt tgtactattg atattttata aaaataatca atgaaactta aagcgtttat
    11641 gactacacta agtggtaaaa ttttaaaagt gattagggaa aacgaataat aaactttgaa
    11701 atttaggagg gacaaagata tgacattaga gatttcaaat gattacggta aaattgatat
    11761 ttcaaacgaa gtgattgctt cggttgtagg tggaaaagcc gttgaatgtt atggtattgt
    11821 aggtatggca tctagacaac aagttagaga tggtattgcg gaaatactag gacatgaaaa
    11881 ttatgctaaa ggcatcaaag taactgaaaa taatggcgta gtgaatatag atatgtacat
    11941 tattgtaagt tacggtgtga aaatatctga agttgccaat aatgtacaat caacagtgaa
    12001 atatactttg gaaaaatcac ttaatgtatc agtaaattca atcaatatat atgtacaagg
    12061 tgtacgtgtg aataatacag gcaagaaagc ttaggaggac aacttgaaat gattagcaaa
    12121 attaatggta aattatttgc cgatatgatt atacaagggg cacaaaattt atctaacaat
    12181 gcagatttgg tagattcttt gaatgtgtat ccagtgccag atggtgatac aggaacaaat
    12241 atgaatctta ctatgacttc aggtcgcgaa gaagtagaga ataatttgtc gaaaaatatc
    12301 ggcgaattag gtaaaacatt ctcgaaaggt ttactaatgg gtgcaagagg taactctggt
    12361 gtcatcttgt cacaattatt cagaggattt tgtaaaaata ttgaaagtga atctgaaatt
    12421 aactcaaaat tgttagctga aagttttcaa gctggtgttg aaacggcata taaagctgtt
    12481 atgaaaccag ttgaaggtac aatacttaca gttgcaaaag atgctgcgca agccgcagta
    12541 gaaaaagcaa ataatactga agattgtata gaattaatgg agtacattat tgtaaaagcc
    12601 aatgaatcac ttgaaaacac accaaactta ttagctgtac ttaaagaagt tggtgttgtt
    12661 gatagtggtg gtaaaggttt gttatgcgtt tacgaaggat tcttaaaagc gcttaaaggt
    12721 gaaaaagttg aagccaaagt tgcaaagata gataaagatg aatttgtaca tgatgaacat
    12781 gatttccatg gtgtaattaa tactgaagat attatttatg gctattgtac cgaaatgatg
    12841 gttcgttttg gaaagaataa aaaagccttt gatgaacaag aattcaggca agatatgagt
    12901 caatttggtg attctttatt agtcattaat gatgaagaaa ttgtgaaagt tcacgtgcat
    12961 accgaacacc caggtaaagt gtttaattat ggtcaacaat atggtgaatt aattaaactt
    13021 aaggttgaaa atatgagaga acagcaccgt gaagtgattc gaaaagaaca gcacacggct
    13081 aaaccgaaaa tggaaacggt tgaaacagca attattacta tttctatggg tgaaggtatt
    13141 tcagagatat ttaaatcaat gggtgccaca catatcatta gtggtggaca aacgatgaat
    13201 ccttctacag aagatatcgt taaagtcatt gaacaatcaa aatgtaaacg tgcaattatt
    13261 ttaccaaata ataaaaatat cttaatggca agtgaacaag cagcgagtat tgttgatgca
    13321 gaagcagttg ttattccaac gaaatctatt cctcaaggta taagcgcact attccaatat
    13381 gatgtggacg caacacttga agaaaataaa gcgcaaatgg ctgattcagt aaataacgtt
    13441 aaatctggtt cattaacgta cgctgttcgt gatacgaaaa ttgatggcgt tgagataaaa
    13501 aaagacgcgt ttatgggctt gattgaagat aagattgtaa gcagccaaag tgatcaatta
    13561 acaacggtta ctgagttgtt aaatgagatg ttagcagaag atagtgaaat attgactgtg
    13621 attattggtc aagatgcaga gcaagaagtt acagataaca tgataaactg gatcgaagag
    13681 caatatccag atgtagaagt ggaagttcat gaaggtggac aaccaattta tcaatatttc
    13741 ttttcagtag aataaaaatt taaaataaaa aactaccaat gataaatcat cagttggtag
    13801 ttttttattt tgctatttta gtgttattgc gggttaaaag tatcattctc gagttgctaa
    13861 caatgtcatg ttcaacttag tcatgataaa ataaataaca tacaaaatga tacgtaaaat
    13921 caaataaaac ataggtgatt tattttggct aaagtaaact taatagaaag tccatattct
    13981 cttttacaat taaaaggtat aggtcctaag aaaatagaag cattgcaaca actaaatatt
    14041 catacagtgg aagatcttgt tctttatttg ccaactagat atgaagataa tacagtgatt
    14101 gatttgaatc aagcagaaga tcaatctaac gttacgatag aaggacaagt atatacagct
    14161 ccagttgttg cattttttgg aagaaataaa tcaaaattaa ccgttcattt aatggtaaat
    14221 aatattgctg tcaaatgtat ttttttcaat caaccgtatt taaaaaagaa aatcgaatta
    14281 aatcaaacta taactgttaa aggtaagtgg aatagggtta aacaggaaat tactggtaat
    14341 agggttttct ttaattcaca agggacacaa actcaagaaa acgcagatgt tcaattagaa
    14401 ccagtctatc gtattaagga aggtattaaa caaaagcaaa tacgagacca aattagacaa
    14461 gcgttaaatg atgtgacaat tcatgaatgg ttaactgatg aactaagaga aaaatataaa
    14521 ttagagacct tggactttac tttgaacacg ttacatcatc caaaaagtaa agaggattta
    14581 ttacgtgctc gtagaaccta tgcatttact gaactgtttt tattcgaatt acgtatgcaa
    14641 tggctaaata gattagaaaa gtcatctgac gaagcaattg aaattgatta tgacttagac
    14701 caagttaaat catttattga tcgtttacct tttgaactaa ctgaagcaca gaaatccagt
    14761 gttaatgaaa tttttagaga tttaaaagca ccaatacgta tgcatcgatt acttcaaggt
    14821 gatgtaggtt caggaaaaac agttgttgct gcaatttgta tgtatgcatt aaaaacggct
    14881 ggttatcaat cagcattgat ggtaccaact gaaattttag cagagcaaca tgctgaaagt
    14941 ttaatggctt tatttggaga ttctatgaac gttgcattgt taactgggtc agtaaaaggt
    15001 aagaaacgaa aaatactttt agaacaactt gaaaatggta cgattgattg tttaattgga
    15061 actcatgcat tgattcaaga tgatgtgatt ttccataatg ttggtttagt aattacagat
    15121 gaacaacatc gatttggtgt gaatcaacgc cagcttttaa gagaaaaagg tgcaatgaca
    15181 aatgtgttat ttatgacagc aaccccgata ccaagaacac tagcaatatc agtttttggt
    15241 gagatggatg tgtcttcaat taaacaatta ccaaaaggtc gtaaacctat cattactact
    15301 tgggcaaagc atgagcaata cgataaagtt ttgatgcaaa tgacttcaga gttgaaaaaa
    15361 ggtcgtcaag catatgtcat ttgcccgctt atagaaagtt ctgagcatct cgaagatgtt
    15421 caaaatgttg tcgcattgta cgagtcttta caacagtatt atggtgtttc ccgtgtaggg
    15481 ttattgcatg gtaagttgtc tgccgatgaa aaagatgagg tcatgcaaaa gtttagcaat
    15541 catgagatag atgttttagt ttctactact gttgttgaag taggtgttaa tgtaccgaat
    15601 gcaactttta tgatgattta tgatgcggat cgctttggat tatcaacttt acatcagtta
    15661 cgcggtcgtg taggtagaag tgaccagcaa agttactgtg ttttaattgc atcccctaaa
    15721 acagaaacag gaattgaaag aatgacaatt atgacacaaa caacggatgg atttgaattg
    15781 agtgaacgag acttagaaat gcgtggtcct ggagatttct ttggtgttaa acaaagtgga
    15841 ttgccagatt tcttagttgc caatttagtt gaagattatc gtatgttaga agttgctcgt
    15901 gatgaagcag ctgaacttat tcaatctggc gtattctttg aaaatactta tcaacattta
    15961 cgtcattttg ttgaagaaaa tttattacat cgtagttttg actaattgcc atgctgattt
    16021 gtcaatttga gtgcaacact tcgttaattg agtgatatga cacttgaact atttaaatgt
    16081 aaagtggtat tttaacaatt tataaatttt cgactaaata atagctaaat aatacagtta
    16141 tttgttgagt cggttaaata gaaagtgtta tgatatgtga ggaatgttta agactaggta
    16201 ctaaaaaatg aggggtgaga cgttgaaact aaagaaagat aaacgtagag aagcaatcag
    16261 acaacaaatt gatagcaatc ccttcatcac agaccatgaa ctaagcgact tatttcaagt
    16321 gagtatacaa acaattcgtt tagatcgcac ttatttaaac ataccagaat taaggaagcg
    16381 tattaaatta gttgctgaaa agaattatga ccaaataagt tctattgaag aacaagaatt
    16441 tattggtgat ttgattcaag tcaatccaaa tgttaaagcg caatcaattt tagatattac
    16501 atcggattct gtttttcata aaactggaat tgcgcgtggt catgtgctgt ttgctcaggc
    16561 aaattcatta tgtgttgcgc taattaagca accaacagtt ttaactcatg agagtagcat
    16621 tcaatttatt gaaaaagtaa aattaaatga tacggtaaga gcagaagcac gagttgtaaa
    16681 tcaaactgca aaacattatt acgtcgaagt aaagtcatat gttaaacata cattagtttt
    16741 caaaggaaat tttaaaatgt tttatgataa gcgaggataa aattatggtt aaattagcaa
    16801 ttgatatgat gggtggcgac aatgcgcctg atatcgtatt agaagccgta caaaaggctg
    16861 ttgaagactt taaagatcta gaaattatac ttttcggtga cgaaaaaaag tataatctga
    16921 accatgaacg aatcgaattt agacattgtt ctgaaaagat tgaaatggaa gatgagcctg
    16981 ttagagcgat taaacgtaaa aaagatagct caatggtaaa aatggctgaa gctgtgaaat
    17041 ctggtgaagc agatggatgt gtgtcagcag gtaatactgg tgctttaatg tcagctggtt
    17101 tattcattgt tggacgtatt aaaggtgtag ctagaccggc tttagtagta acattgccaa
    17161 cgattgatgg aaaaggtttt gtctttttag acgttggtgc aaatgctgat gctaaacctg
    17221 aacacttatt acagtatgcg caactagggg atatttatgc tcaaaaaatt agaggtattg
    17281 ataatccgaa aatctcatta ttaaatatag gaaccgagcc agctaaaggt aatagtttaa
    17341 cgaaaaaatc atatgagtta ttaaatcatg atcattcatt gaattttgtt gggaatattg
    17401 aagcgaagac attaatggat ggcgatacag atgttgtagt taccgatggc tatacaggga
    17461 acatggtcct taaaaattta gaaggtactg caaaatcaat cggtaaaatg ttaaaagata
    17521 cgattatgag tagtactaaa aataaattag caggtgcaat attgaagaaa gatttagctg
    17581 aattcgctaa aaagatggac tactcagaat acggtggttc cgtattatta ggattggaag
    17641 gtactgtagt taaagcacac ggtagttcaa atgctaaagc tttttattct gcaattagac
    17701 aagcgaaaat cgcaggagaa caaaatattg tacaaacaat gaaagagact gtaggtgaat
    17761 caaatgagta aaacagcaat tatttttccg ggacaaggtg cccaaaaagt tggtatggca
    17821 caagatttgt ttaacaacaa tgatcaagca actgaaattt taacttcagc agcgaacaca
    17881 ttagactttg atattttaga gacaatgttt actgatgaag aaggtaaatt gggtgaaact
    17941 gaaaacacgc aaccagcttt attgacgcat agttcggcat tattagcagc gctaaaaaat
    18001 ttgaatcctg attttactat ggggcatagt ttaggtgaat attcaagttt agttgcagct
    18061 gacgtattat catttgaaga tgcagttaaa attgttagaa aacgtggtca attaatggcg
    18121 caagcatttc ctactggtgt aggaagcatg gctgcagtat tgggattaga ttttgataaa
    18181 gtcgatgaaa tttgtaagtc attatcatct gatgacaaaa taattgaacc agcaaacatt
    18241 aattgcccag gtcaaattgt tgtttcaggt cacaaagctt taattgaaga gctagtagaa
    18301 aaaggtaaat cattaggtgc aaaacgtgtc atgcctttag cagtatctgg accattccat
    18361 tcatcgctaa tgaaagtgat tgaagaagat tttgcaagtt acattaatca atttgaatgg
    18421 cgtgatgcta agtttcctgt agttcaaaat gtaaatgcgc aaggtgaaac cgacaaagaa
    18481 gtaattaaat ctaatatggt caagcaatta tattcaccag tacaattcat taactcaaca
    18541 gaatggctaa tagaccaagg tgttgatcat tttattgaaa ttggtcctgg aaaagtttta
    18601 tctggcttaa ttaaaaaaat aaatagagat gttaagttaa catcaattca aactttagaa
    18661 gatgtgaaag gatggaatga aaatgactaa gagtgcttta gtaacaggtg catcaagagg
    18721 aattggacgt agtattgcgt tacaattagc agaagaagga tataatgtag cagtaaacta
    18781 tgcaggcagc aaagagaaag ctgaagcagt agtcgaagaa atcaaagcta aaggtgttga
    18841 cagttttgcg attcaagcaa atgttgccga tgctgatgaa gttaaagcaa tgattaaaga
    18901 agtagttagc caatttggtt ctttagatgt tttagtaaat aatgcaggta ttactcgcga
    18961 taatttatta atgcgtatga aagaacaaga gtgggatgat gttattgaca caaacttaaa
    19021 aggtgtattt aactgtatcc aaaaagcaac accacaaatg ttaagacaac gtagtggtgc
    19081 tatcatcaat ttatcaagtg ttgttggagc agtaggtaat ccgggacaag caaactatgt
    19141 tgcaacaaaa gcaggtgtta ttggtttaac taaatctgcg gctcgtgaat tagcatctcg
    19201 tggtatcact gtaaatgcag ttgcacctgg ttttattgtt tctgatatga cagatgcctt
    19261 aagtgatgag cttaaagaac aaatgttgac tcaaattccg ttagcacgtt ttggtcaaga
    19321 cacagatatt gctaatacag tagcgttctt agcatcagac aaagcaaaat atattacagg
    19381 tcaaacaatc catgtaaatg gtggaatgta catgtaatat atttgagcta aagctcaaga
    19441 taaagacgta gtaatcaata aattgataac agggatagag gtacgcctga gctaaagctc
    19501 attgacgcag tggttgactg gtcatccgat ggagaattgc ctgacctagt caactttgcg
    19561 gggggaattc taagtaacct agataaggtt ccagaatttc tccctaagaa acactaatca
    19621 ataaattgat aagaagatta gaggaacgct tgagctaaag ctcatgcata agaaacagta
    19681 atcaataaat tgataactgg gatagaggta cgcctgagct aaagctcatg cataagaaac
    19741 agtaatcaat aaattgataa ctgtttctaa aatttctact tgttttttag aatttaaaat
    19801 gggaaaatat aatagtctat gtataggcat ttttaaagga ggtgaatcga cgtggaaaat
    19861 ttcgataaag taaaagatat catcgttgac cgtttaggtg tagacgctga taaagtaact
    19921 gaagatgcat ctttcaaaga tgatttaggc gctgactcac ttgatatcgc tgaattagta
    19981 atggaattag aagacgagtt tggtactgaa attcctgatg aagaagctga aaaaatcaac
    20041 actgttggtg atgctgttaa atttattaac agtcttgaaa aataataaat cttacatctg
    20101 ggtcgtcagt attgtcgact cagttttttt ctttaattat caatagtttt aacgtaaaat
    20161 taaagatgat tcaagagcaa cacataaagg agataaaata atgtctaaac aaaagaaaag
    20221 tgagatagtt aatcgtttta gaaagcgctt tgatactaaa atgacagagt taggctttac
    20281 ttatcaaaat attgatttat accaacaagc attttcgcat tcgagtttta ttaatgattt
    20341 taatatgaat cgtttagacc ataatgagcg tttagagttt ttgggtgatg cggtattaga
    20401 attgacggtt tcacgatatt tatttgataa acatcccaac ttgccagaag ggaatttaac
    20461 aaaaatgcgt gccactattg tatgtgagcc ctcacttgta atatttgcga ataaaattgg
    20521 attgaacgaa atgattttac ttggtaaagg tgaagagaaa acagggggac gtacaagacc
    20581 atcattaata tcagatgcat tcgaagcatt tattggggca ttgtatttgg atcaaggact
    20641 agatatagtt tggaaatttg ctgagaaagt tattttccca catgtagaac aaaatgagtt
    20701 attaggagtg gtagatttta aaacacaatt ccaagaatat gtgcaccagc aaaataaagg
    20761 tgatgtaacc tataatttaa taaaagaaga gggaccggca catcatcgtc tattcacttc
    20821 agaagttatt ctgcaagggg aagcaatagc tgaaggtaaa gggaaaacga aaaaagaatc
    20881 agaacaacgt gctgctgaaa gtgcctataa gcaattaaaa caaattaaat agaagtttat
    20941 atgatacgac atataggtgt tcgatttagg atttaaggaa gaatttttaa tattttatgg
    21001 aacaacttcg aaatttaata tacaaatatt aaaatatcgt atacatgtgt tctttaaaat
    21061 tgtgataagg agtttaggat ggtttattta aaatcaatag atgccattgg atttaagtct
    21121 tttgcagatc aaaccaatgt tcaattcgat aaaggtgtaa ctgcaattgt tggtccaaat
    21181 ggaagcggta aaagtaatat tacagatgct attaaatggg tgttgggtga acaatcggct
    21241 aaatcattac gtggctcaaa aatggaagat attatcttct caggtgcaga acatcgcaaa
    21301 gctcaaaatt atgctgaagt acagttaaga ttagataatc attctaaaaa gctcagtgtt
    21361 gatgaaaacg aagttattgt aacaagaaga ttgtatcgaa gtggtgaaag tgagtactac
    21421 ataaataatg accgtgcaag attaaaagat attgccgatt tatttttaga ttctggattg
    21481 ggaaaagaag catatagcat tatctcgcaa ggtagagttg atgaaatact aaatgctaaa
    21541 ccaattgata gacgtcaaat tattgaagaa tcggctggtg tacttaaata taaaaaacgt
    21601 aaagctgaat cattaaataa acttgaccaa acagaagata atttaacgag agtagaagac
    21661 attttatatg atttggaagg tcgtgtagaa cctctaaaag aggaggcagc tatagctaaa
    21721 gaatataaga cactttcaca ccaaatgaaa catagtgaca ttgtagttac agtgcacgat
    21781 attgatcaat atacaaatga caatagacaa ttagatcaac gtttaaatga tttacaaggt
    21841 caacaagcaa ataaagaagc tgacaagcaa cgtttaagcc aacaaattca acaatataaa
    21901 ggtaaacgtc atcaacttga taatgatgtt gaatcgctta attatcaatt agtaaaagct
    21961 acggaagcct ttgaaaaata tacgggacaa ttaaatgttt tagaagaacg taagaaaaat
    22021 caatctgaaa caaatgcacg atatgaagaa gaacaagaaa atttaatgga gcttttagaa
    22081 aatatatcaa atgagatttc tgaagctcaa gatacttata agtctctgaa aagtaaacaa
    22141 aaagaactca atgctgtcat tcgtgaactt gaagaacaac tatatgtttc agacgaagca
    22201 catgatgaaa aattggaaga aattaaaaac gaatactata cattaatgtc agagcaatca
    22261 gatgttaaca atgatattcg ttttttaaag catactatag aagagaatga ggctaaaaaa
    22321 tcaagactag attctcgatt agttgaagtt tttgagcaat tgaaagatat tcagggtcaa
    22381 ataaaaacga caaaaaaaga atatcaacag accaacaaag aactttctgc tgtagataaa
    22441 gaaattaaaa atatagaaaa agatctcact gatacaaaaa aagcacaaaa tgaatacgaa
    22501 gagaaattgt atcaagcata tcgatatacc gaaaaaatga aaacacgtat tgatagtttg
    22561 gcaacgcaag aggaagaata tacttatttt ttcaatggcg tcaaacatat tttgaaagct
    22621 aaaaataaag aattaaaggg tattcatggt gcagttgcgg aaattattga tgtgccatct
    22681 aaattaactc aggcaattga aacagcatta ggtgcttcat tacaacatgt cattgtagat
    22741 tcagaaaaag atggacgcca ggctattcaa tttttaaaag aacgtaattt aggtcgtgcg
    22801 acgtttttac cattaaatgt tatacagagt agagtggtag cgactgatat taaatctatt
    22861 gctaaagagg caaacggatt tattagtatc gcttcggaag cagttaaagt agcaccagaa
    22921 tatcaaaata ttatcgggaa tttattaggt aatacgatta tcgttgatca tttaaagcat
    22981 gcaaatgaat tggcacgtgc gattaaatat cgaactcgta ttgttacttt ggaaggtgat
    23041 attgtaaatc ctggtggttc tatgactggt ggtggcgctc gtaagtcaaa aagtattctg
    23101 tctcaaaaag acgagttgac aacaatgaga caccaattag aagattactt gcgtcaaaca
    23161 gaatcatttg aacaacaatt taaagagttg aagataaaaa gtgatcaatt aagtgaactg
    23221 tattttgaaa aaagtcaaaa gcataataca cttaaagagc aagtgcatca ttttgaaatg
    23281 gagctcgata gattaactac acaagaaaca caaataaaaa atgatcatga agaattcgaa
    23341 tttgaaaaaa atgatggtta tacgagtgac aaaagtcgac aaactttgag tgaaaaagaa
    23401 actcatctag aaagtattaa agcatcttta aaacgactag aagatgaaat tgaacgctac
    23461 acaaaacttt ctaaagaagg taaggaaagc gttactaaaa cacaacaaac cttacatcag
    23521 aaacaatctg atcttgctgt ggttaaagag cgtattaaaa cacaacaaca gacaatagat
    23581 cgattaaata atcaaaatca acaaactaaa catcaattaa aagatgttaa agaaaaaatt
    23641 gcattcttta attcggatga agtgatgggc gaacaagctt ttcaaaatat taaagatcaa
    23701 attaatggtc aacaagaaac gagaacacgc ttatctgatg aattagataa attgaaacaa
    23761 caacgtattg agttgaatga acaaatcgat gcgcaagaag ctaaactaca agtttgtcac
    23821 caagatattt tagctatcga aaatcactac caagatatta aagctgaaca atcaaagcta
    23881 gatgtattaa ttcatcatgc gatagatcat ttaaatgatg aatatcaatt gactgttgaa
    23941 cgtgcgaaat ctgaatatac gagtgatgaa tcgattgacg cattacgtaa aaaagttaag
    24001 ttaatgaaga tgtcgattga tgaactaggt cctgtaaact taaatgcaat tgaacaattt
    24061 gaagagttaa atgaacgtta tacattttta agtgaacaac gtacagatct tcgtaaagct
    24121 aaagaaacat tagagcaaat tataagtgaa atggatcaag aggttactga aagatttaaa
    24181 gaaactttcc atgctattca aggacatttt acagctgtgt tcaaacaatt gtttggtgga
    24241 ggcgatgcag aattgcaatt aactgaagcc gattatttaa cagctggtat tgatattgtg
    24301 gtacaaccac cgggtaaaaa gttgcaacat ttatcgttac taagtggtgg tgagcgtgca
    24361 ttaactgcta ttgctttact atttgcaatt ttaaaagtaa gatctgcacc ttttgttata
    24421 ttagatgagg ttgaagctgc attagatgaa gcgaatgtta ttagatacgc aaaatattta
    24481 aatgagttat cagacgaaac acaattcatt gttattacac accgtaaagg aacaatggaa
    24541 tttgctgata ggttatacgg tgtgacaatg caagaatcag gtgttactaa acttgtgagt
    24601 gtgaatttaa atacaataga tgatgtgttg aaggaggagc aataatgagc ttttttaaac
    24661 gcttaaaaga taaatttgca acaaataaag aaaatgaaga agttaaatcc ttaacagaag
    24721 aacaaggtca agacaaatta gaagatacac attctgaagg ttcaacgcag gacgtaaatg
    24781 atttagcaga aaatgctgaa gtgaaaaaga agccacgcaa gttaagtgaa gcggattttg
    24841 atgacgatgg cttaatatca attgaagatt ttgaagaaat tgaagctcaa aaaatgggtg
    24901 ctaaatttaa ggcaggactc gaaaaatctc gtcaaaattt ccaagaacaa ttaaataatt
    24961 tgatagcgag atatcgtaaa gtagatgaag acttttttga agctttagaa gaaatgttaa
    25021 tcactgcaga cgtcggtttt aatactgtga tgacgttaac tgaagaatta cgtatggaag
    25081 cacaacgacg taatattcaa gatactgaag atttgcgtga agtcattgtt gaaaaaatcg
    25141 tagagattta ccatcaagaa gatgataatt cagaagctat gaacttagaa gatggtcgtt
    25201 taaatgtcat tttaatggtt ggtgtgaatg gtgttggtaa aacaacaaca attggaaaat
    25261 tagcttaccg atataaaatg gaaggtaaaa aagtaatgtt agctgcgggc gatactttta
    25321 gagcgggtgc tattgatcaa ttgaaagttt ggggcgaacg tgttggtgta gatgtaatta
    25381 gccaaagtga aggttctgat ccagctgctg ttatgtatga tgcgattaat gccgctaaaa
    25441 acaaaggtgt tgatatttta atctgtgata ccgctggacg tttacaaaat aaaacaaatc
    25501 taatgcaaga attagaaaaa gttaagcgtg taattaatcg agcagtgcca gatgcgcctc
    25561 atgaagcatt actatgttta gatgctacaa ctggtcagaa tgcgttgtca caagctagaa
    25621 actttaaaga agtaacaaat gttacaggta ttgtattaac gaaattagat ggtacagcca
    25681 aaggtggtat cgtattagcc attcgtaatg aattgcacat tccagttaaa tatgtaggtt
    25741 taggtgagca attagatgac ttacaaccat ttaaccctga aagttatgtc tacggcttat
    25801 tcgctgatat gattgaacaa aatgaagaaa taacaacagt tgaaaatgat caaattgtaa
    25861 cagaagaaaa ggacgataat catggggcaa aatgatttag ttaaaacgtt acgaatgaat
    25921 tatttgtttg atttttatca atccttattg acgaataaac aacgtaatta tttggaatta
    25981 ttttatctcg aagattattc tttgagtgaa atcgcagata cttttaatgt gagtagacaa
    26041 gcagtttatg ataatataag aagaactggc gatttagttg aagattatga aaagaaattg
    26101 gaattatacc agaaatttga gcaacgccga gaaatatatg atgaaatgaa acaacattta
    26161 agtaatccag aacaaataca acgttatatt caacaattag aagacttaga atagatataa
    26221 attgagggag gacatcgata tggcatttga aggcttatca gaacgcctgc aagcgacgat
    26281 gcaaaaaatg cgtggtaagg gtaaacttac tgaagctgat ataaagataa tgatgcgtga
    26341 agtaagatta gcgttacttg aggctgacgt aaactttaaa gtggtaaaag aatttattaa
    26401 aacagtatca gaacgcgcat taggttccga tgtaatgcaa tcattaacac ctgggcaaca
    26461 agttattaaa atagttcaag atgaattaac gaagttgatg ggtggagaaa attcatcgat
    26521 taatatgtca aataaaccac ctactgttgt tatgatggtt ggtttacaag gtgctggtaa
    26581 aacaacaact gcaggtaaat tagcattatt gatgcgtaaa aaatacaaca aaaaacctat
    26641 gttagttgca gcagatattt atcgtccagc agcgataaat caattacaaa cagtagggaa
    26701 acaaattgat attcctgtat acagtgaagg ggatcaagta aagccacaac aaattgtaac
    26761 taatgcatta aaacatgcta aagaagaaca tttagacttt gtaatcattg atacagcagg
    26821 tcgattacac atcgatgaag cattgatgaa cgaattaaaa gaagtaaaag acattgctaa
    26881 accaaacgaa attatgttag ttgtcgattc aatgacgggt caagatgctg tcaatgttgc
    26941 agaatctttt gacgatcaac ttgatgtcac aggtgttacc ttaactaaat tagatggtga
    27001 tacacgtggt ggtgcagctt tatctattcg ttcggtgaca caaaaaccaa ttaaatttgt
    27061 tggtatgagt gaaaagttag atggtttaga gctattccat cctgaacgta tggcatcacg
    27121 tattttaggt atgggtgatg tgttaagttt aattgaaaaa gcgcaacaag atgtggatca
    27181 agaaaaagca aaagatttag agaaaaagat gcgtgaatca tcatttactt tagatgattt
    27241 tttagaacag cttgatcagg tgaaaaattt aggaccactg gatgatatta tgaaaatgat
    27301 tccaggtatg aataaaatga aaggtctaga taagcttaat atgagtgaaa agcaaattga
    27361 tcatattaaa gcgattatcc agtcaatgac gccggctgaa agaaacaatc cagacacatt
    27421 gaatgtatca cgtaaaaagc gtattgctaa aggttctggt cgttcattac aagaagtcaa
    27481 tcgtttgatg aaacaattta acgatatgaa gaaaatgatg aaacaattca ctggtggcgg
    27541 taaaggtaaa aaaggtaaac gcaatcaaat gcaaaatatg ttaaaaggta tgaacttacc
    27601 attttaatgt ttaatttaac acgctgattt ttgttgcaat gagatgtgca tcccgaaagt
    27661 tatactaaag aatataactt tatggggtgc atatttttag atacacaata ataattaaaa
    27721 actgaaattg ctgatttgct agaacatgag gttattgttg ctaatattaa taagttatat
    27781 tggtggaaca tgtgcgttag aagtggtacc attcatgtcc ttacgagata aattaactta
    27841 acacgtatgt ggtgaaggtt aatatgaatt tttataaact attaacagtc taatttagta
    27901 atcacatagt tatttttgta agaaaaaggt tcaataattt tcctggtaaa gaaaaaactc
    27961 tttacaaaca ttcacacacc tgttaatatt atttcttgta gaaaataaaa attaaaacat
    28021 gacttaaagg agattttata aatggcagtt aaaattcgtt taacacgttt aggttcaaaa
    28081 agaaatccat tctatcgtat cgtagtagca gatgctcgtt ctccacgtga cggacgtatc
    28141 atcgaacaaa tcggtactta taacccaacg agcgctaatg ctccagaaat taaagttgac
    28201 gaagcgttag ctttaaaatg gttaaatgat ggtgcgaaac caactgatac agttcacaat
    28261 atcttatcaa aagaaggtat tatgaaaaaa tttgacgaac aaaagaaagc taagtaattt
    28321 agcgtaaaat tgttctaaca ataagaataa ctcgtttaca ctgacagtta ttactcaatg
    28381 atacgttggg aatatcacat gttagtaata tagaacgtat tgggtaccat aatggtgccc
    28441 tttttctttg aataattttc aattaaaata gaagtgttca aagcatagag ttggaggtaa
    28501 tagaatgaga gttgaagttg gtcaaattgt taacacacat ggtattaaag gtgaaattaa
    28561 agtaaaatcc aattcagact ttacagacgt tcgttttcaa cccggtcaag tgctgacagt
    28621 tgtgcataac aataacgacc ttgaatatac tgttaagtca catagagtgc ataaagggct
    28681 tcatatgctt acatttgaag gtattaataa tattaatgat attgagcatt taaaagggag
    28741 ttctatttat caagagcgtg atcatgaaga tatcgtactt gaggaaaatg aattttatta
    28801 ttcagatatt ataggatgta cagtttttga tgatcaagaa acaccaatag gtcgtgtaat
    28861 taatatcttt gaaacaggtg caaatgatgt gtgggtgatt aaaggatcta aagaatattt
    28921 gattccttat attgctgatg ttgtaaaaga agtggatgtt gaaaataaaa aaattatcat
    28981 cacgccaatg gaaggattgt tggattaatg aaaattgatt atttaacttt atttcctgaa
    29041 atgtttgatg gtgttttaaa tcattcaatt atgaaacgtg cccaagaaaa caataaatta
    29101 caaatcaata cggttaattt tagagattat gcaattaaca agcacaacca ggtagatgat
    29161 tatccgtatg gtggcggaca aggtatggtg ttaaagcctg aacctgtttt taatgcgatg
    29221 gaagacttag atgtcacaga acaaacacgc gttattttaa tgtgtccaca aggcgagcca
    29281 ttttcacatc agaaagctgt tgaattaagc aaggctgacc acatcgtttt catatgcgga
    29341 cattatgaag gttacgatga acgtatccga acacatcttg tcacagatga aatatcaatg
    29401 ggtgactatg ttttaacagg tggagaattg ccagcgatga ccatgactga tgctattgtt
    29461 agactgattc caggtgtttt aggtaatgaa cagtcacatc aagacgattc attttcagat
    29521 gggttattag agtttccgca atatacacgt ccgcgtgaat ttaaaggtct aacagttccg
    29581 gatgttttat tgtctggaaa tcatgccaat attgatgcat ggagacatga gcaaaagttg
    29641 atccgcacat ataataaaag acctgactta attgaaaaat atccattaac taatgcagat
    29701 aagcaaatat tagaaagata taaaatagga ttgaaaaaag gttagtattg tgctaacata
    29761 atctgagtgt ggaaacacaa aaatcactat gatccgctgc tatatatttg tcgaggcaag
    29821 aacataggtt gaagaggaga atttataatg acaaatcaca aattaatcga agcagtaact
    29881 aaatcacaat tacgtacaga cttaccaagt ttccgtcctg gtgatacttt acgtgtacac
    29941 gtacgtatca ttgagggtac tcgtgagcgt atccaagtat tcgaaggcgt tgtaattaaa
    30001 cgtcgtggcg gtggcgtttc tgaaacgttt acagttcgta aaatttcatc aggtgttggc
    30061 gtggaacgta cattcccatt acacacacca aaaattgaaa aaatcgaagt taaacgtcgt
    30121 ggtaaagtac gtcgtgctaa attatattac ttacgtagtt tacgtggtaa agctgctaga
    30181 atccaagaaa ttcgttaatc agcatttaaa caaagctatg tatgagtcat attcgactca
    30241 aacaacaaag ccatctaggt cacttttatg gagtgatata gatggctttt tttgctgttt
    30301 agttaatgtg aagttgataa tgtaattgtt tttttattta ttcataacgt gtttgctcaa
    30361 aatggttata taaacaaaaa tctactaagt gccattgaaa aggacttagt agatttatta
    30421 aatatatgcg tagccacatt atttttgtct tacccatctc gtgaaaataa tactacatat
    30481 aatgccaaat atagtaattg ttataagtaa ataatagtat ggtggggtat agctcaattg
    30541 aatctttgtt atatttttag gtgctttaat gccggtcata acaccattta cttgttcaac
    30601 tttaagactt tgatcacctg atgtcgcttt cataccttga ttatatgctg ttggcaaaac
    30661 aatataccca gatgaatttt tatttttagt aatagtataa ccttgctttg tcttactaac
    30721 tttgacagcc tctaatgaat ttgaagtgtc tttaagcgtg gtataatctt cgccgtatat
    30781 cccttttaaa tttacacgat acttaccttt aggcaacgat aatttaattg catctgaaga
    30841 tttaatacgt atcgttacag gtgttacaaa acgacggtat ttatatgtga gtttgtttct
    30901 ttctagtgta tattcattaa ctttaacata atgagcttta tccggcgaaa gtaattctaa
    30961 atccatttca aaatacaaat ctttaaattg attagaaact gattttggca actgtacagt
    31021 tagaccacca ttattttgtt taacttgtaa taaatgtttt gaaggagatt gccaggctgc
    31081 actatttaat ttaattgttg aatctgatag taaattttta ttggctttaa aatgtgtatt
    31141 tacatcttta gtattgttag aaacaatccc ttgcaacatt gcttgttctt tatctaatgg
    31201 agattttaat tctttattgg aaaagacctt atttgtaata tgtgcacttg gataatggat
    31261 ggtattttta gaatgaatcc aacgaacttt attgtctttg tgttcagagt taattttaaa
    31321 tccatatggt aagttgtaat catgatttac tctaattcga tcatttacat tccaaagcga
    31381 tagtaaattt tgacgattgc caagtaatct ataagtgctg tttttatcga ttggcatatt
    31441 aatttggagt gtcttgtcat aatattttaa aatgtctcca ttaaaaatac tagaatataa
    31501 tgaaatgcca ttataatgat atataaatgg tgaatttaat gcatagtctg acatataatc
    31561 aatgcgatta aatgggcctg ttgcattttg atttatcttt tttataagct ggtttacata
    31621 gttactatga taatcatgtt gtttcaacgt tgatattgat tgttgataag gtttgattgc
    31681 catatttttg ttgttatcta aaatgacgat ttgttgaatc ataacgatta atactattaa
    31741 tacaacggtt aattttttat aacgccataa actaaatttt aaaataacgg caagcaccat
    31801 tagcaggata atacctacta taagtacgag tgggtgtgtc ggtgatagta atacataaag
    31861 taatgcgatg atgcttactg gtattgttct gattaaataa tatttcatat ttaatgttga
    31921 taaatgctga ataaacaatc cgcaaagagc acttgatgat agtgctaaga tatacaccca
    31981 acgcctttct ggaaatgaaa aaccattaaa agcactgtcg aaatactgtg ataatgaacc
    32041 aataaataat atccatgtta ctattgcgaa aagtctataa aagtaaaaac gatacagttt
    32101 gaatgacaat aatgcaacga tagtaagaat tgaaatcgta atataaaatc catcgctaaa
    32161 gaaaaaataa tgataatcaa gtggtgtcaa aaacggtata tcaacattgg gattttgctt
    32221 tctgtcattt tccaaaaacg cagaaatgcc agtgaataaa ccaaatacac ttgataacac
    32281 actcaaaact gcagcagata tgacgcaaat taatttttgt gttctagaga caatgtcata
    32341 tttgtaagtg aaaatgagtc gatataaata gtagcaacct ataataatag cttgataata
    32401 actgaaataa aaattgctaa atagtgttaa ggctatcgca acaatgaata taccgatttt
    32461 gcgttgttga aaatatcttt ccaaaccaag aatcgataat ggtaataaat ataataaatt
    32521 tccataaaat gaccaagtaa aattaaagta tataacgaca gttgacatgc cgtataaaat
    32581 cgtagcgatc atatttgttg agcgtttaaa gtgtaatatt ttaaataagt agaaggtcac
    32641 gacaaatgtt atgatagctc gtatcatggc cataataagt tggtttgtcg gccaaaaatg
    32701 tattgttgtc ggattaaata taccaaccgt ttctcctatt ttaatgaata gaaaatttag
    32761 ccacattaaa ggtgacagcg aataataata tgatagtcct ttcatataat cgccacctaa
    32821 tccaaacgat gcatcatata aactagaaaa actacttaga tgttcataca aatacatttg
    32881 aaatggcatc atttgacgga atccatctcc agccccacta aaaacagtac cattcacaat
    32941 ataatcatag atatgagtag aaaataaaat aagcgttaat attacactaa tgaaagttat
    33001 aacaaagaat tgtttgacgt ttgaatttag ccactttttt aacacaacat tatcctcaac
    33061 tttcaaattt aaaattaagt ttaactgaaa ctaaagttaa tgaggttctt gataggtaaa
    33121 gacgaagatg actgtggaac agatacctta tcatagttac ttaaactttg gatcattttc
    33181 agtttatcat taaacaaata tattgaataa taaaaatgtc atactgataa agatgaatgt
    33241 cacttaataa gtaacttaga tttattaaat gaaaattttt aattagagaa aacttggaat
    33301 aatcacttat acctgaatct aaagcattgt taagaagtgt gacaatgtta aaataaatat
    33361 agttgaatta atgaatttgt tctataatta acaggttata gattttaata atgagaaaag
    33421 aattgacgaa agtaaggtga attgaatggt tattcaatgg tatccaggac atatggcgaa
    33481 agccaaaaga gaagtaagtg aacaattaaa aaaagtagat gtagtgtttg aactagtaga
    33541 tgcaagaatt ccatatagtt caagaaaccc tatgatagat gaagttatta accaaaaacc
    33601 acgtgttgtt atattaaata aaaaagatat gtctaattta aatgagatgt caaaatggga
    33661 acaatttttt attgataaag gatactatcc tgtatcagtg gatgctaagc acggtaaaaa
    33721 tttaaagaaa gtggaagctg cagcaattaa ggcgactgct gaaaaatttg aacgcgaaaa
    33781 agcaaaagga cttaaaccaa gagcgattag agcaatgatt gttggaattc caaatgttgg
    33841 taagtccaca ttaataaata aactggcaaa gcgtagtatt gcacagactg gtaataaacc
    33901 aggtgtgacc aaacaacaac aatggattaa agttggtaat gcattacaac tattagatac
    33961 accagggatt ctttggccta aatttgaaga tgaagaagtc ggtaagaagt tgagtttaac
    34021 aggcgcgatt aaagatagta ttgtgcactt agatgaagtt gccatctatg gtctgaactt
    34081 tttaattcaa aatgatttag cgcgcttaaa agcacattat aaaatcgaag ttcctgaaga
    34141 tgcagaaatc atagcgtggt ttgatgcgat agggaaaaaa cgtggcttaa ttcgacgtgg
    34201 taatgaaatt gattacgaag cagtcattga actgattatt tatgatattc gaaatgctaa
    34261 aataggaaat tattgttttg atatttttaa agatatgact gaggaattag caaatgacgc
    34321 taacaattaa agaagtaacg cagttgatta atgcggttaa tacaatagaa gaattagaaa
    34381 atcatgaatg ctttttagat gagcgaaaag gtgtacaaaa tgccatagcc agacgcagaa
    34441 aagcgttaga acaagaacaa gctttaaaag aaaagtatgc tgaaatgact tactttgaaa
    34501 atgaaatatt aaaagagcat cctaatgcta ttatttgtgg tattgacgaa gttggaagag
    34561 gacctttagc aggtccagtc gttgcatgcg caacaatttt aaattcaaat cacaattatt
    34621 tgggccttga tgactcgaaa aaagtacctg ttacgaaacg tctagaatta aatgaagcac
    34681 taaaaaatga agttactgct tttgcatatg gtatcgctac agctgaagaa atagatgaat
    34741 ttaatattta taaagctact caaatcgcca tgcagcgagc tattgatgga ttatcagtac
    34801 aaccaacgca tttattgata gacgcgatga cgcttgataa tgcactgact caagtatctt
    34861 taatcaaggg tgatgcaaga agtgtatcta ttgcagcggc aagtatcatg gcaaaggttt
    34921 ttcgtgatga ttatatgaca cagttatcta aagattatcc tgaatatggt tttgaaaaaa
    34981 acgcgggtta cggtaccaaa caacatttac tagcaatcga tgatattggc attatgaaag
    35041 agcatagaaa aagctttgaa cctataaaat cgttactgta aatcgtaaaa ttcaattaaa
    35101 tgataaaaaa gtagcattta agtttacaat agcgcttaca ttttttataa ttgtaaggga
    35161 acttaaccta agtaacagga ggatggaaga tgaatatcca cgagtatcaa ggtaaagaaa
    35221 tatttcgttc aatgggcgtt gcagttccag aaggacgagt agcatttact gctgaagaag
    35281 cggtggagaa agcaaaagaa ttaaattcag atgtttatgt tgtaaaagca caaattcatg
    35341 ctggaggtag aggtaaagca ggcggagtaa aaattgctaa atctttatca gaggtagaaa
    35401 catatgcaaa agaattatta gggaaaactt tggtgacaca tcaaactggt ccagaaggta
    35461 aagaaattaa gcgtttatat atcgaagaag gttgtgctat tcaaaaagaa tattacgttg
    35521 gattcgttat tgatcgtgcg actgaccaag taacattgat ggcgtctgaa gaagggggca
    35581 ctgagattga agaagttgct gcgaagactc ctgaaaagat cttcaaagaa actattgatc
    35641 cagtaatcgg actttcacca ttccaagcaa gacgaattgc gtttaatatt aatattccta
    35701 aaaaatctgt taacaaagct gctaaattct tattagcact ttataatgta ttcattgaaa
    35761 aagattgttc aatcgtagaa atcaacccat tagttacaac agctgatggt gatgtattgg
    35821 cattagatgc taaaattaat tttgatgata atgcattatt cagacataaa gatgttgtag
    35881 aattacgtga tttagaagaa gaagatccga aagagattga agcgtctaaa catgatttat
    35941 catacattgc attagatggt gacatcggat gtatggttaa tggtgcaggt ttagccatgg
    36001 caacaatgga tacgattaat catttcggtg gaaacccagc caatttctta gatgcaggcg
    36061 gaagcgctac tagagaaaaa gtaactgaag catttaaaat cattttaggt gatgaaaatg
    36121 ttaaaggtat ttttgtaaac attttcggtg gcattatgaa atgtgatgtt atcgcagaag
    36181 gtatcgttga agctgtaaaa gaagtagatt taactttacc actagttgta cgcttagaag
    36241 gtacaaatgt tgagttaggt aaaaaaatct taaaagactc aggattagca attgaaccag
    36301 cagcaacaat ggctgaaggt gcacaaaaaa ttgttaaact agtcaaagaa gcataaggaa
    36361 aggatgggag cactaagatg agtgtattta tagataagaa tactaaagta atggtacaag
    36421 gtattacagg gtctactgcc cttttccata caaaacaaat gcttgattat ggtacgaaaa
    36481 tagtagcagg tgtgacgcct ggtaaaggtg gtcaagttgt tgaaggcgtt cctgttttca
    36541 acactgttga agaagctaaa aatgaaaccg gggcaacggt ttcagtcatt tacgttccag
    36601 caccatttgc tgcagactca attttagaag cagctgatgc agacttagat atggttattt
    36661 gtatcactga acatattcct gtattagaca tggttaaagt taaacgctac ttacaaggta
    36721 gaaaaacacg tttagttggt ccgaactgtc caggtgtgat tacagcagat gaatgtaaaa
    36781 ttggtattat gcctggctat attcacaaaa aaggtcatgt tggtgtagta tcacgttcag
    36841 gtacattaac atatgaagca gtgcaccaat tgactgaaga aggtattggt caaactacag
    36901 ctgttggtat tggtggagac ccagtaaatg gcacaaactt tattgatgtt ttaaaagcat
    36961 tcaatgaaga tgacgaaacg aaagcagttg ttatgattgg tgaaatcggt ggtacggctg
    37021 aagaagaagc tgctgaatgg attaaagcga atatgacaaa accagttgta ggctttatcg
    37081 gtggacaaac agcacctcct ggaaaacgta tgggacatgc tggtgcaatc atttcaggtg
    37141 gtaaaggtac tgctgaagag aaaattaaaa cattaaatag ttgtggtgtg aaaacagcgg
    37201 caacaccttc agaaattggt tcaacattaa ttgaagctgc taaagaagca ggaatttatg
    37261 aatcattatt aactgttaat aaataaagtt aaaagatgat ataaatggct atagcccatt
    37321 ccatacttta ttaaagtatc ggaaatgggc tatttgtcgt gcgatgaatt ttagtacact
    37381 tttcatattt tggaaaggca agttaattat tttacctcct tcaatagctt tctaaaacaa
    37441 agtgaatttt tattataata tatagtgcgc tatgttataa tatttatata ttattgcaag
    37501 gagtgtttca tcatgaataa acaacaaagt aaagtacgct attcaattag aaaagttagt
    37561 attggaattt tgtcaatttc aattggtatg tttttggcat tgggtatgtc gaacaaagca
    37621 tatgcagatg aaattgataa atctaaagat tttacaagag ggtatgagca aaatgtattc
    37681 gcgaaatcag agttaaatgc taataaaaat acgacaaaag acaaaataaa aaatgaaggt
    37741 gttgttaaaa catcggacac aagtttaaag ttagacaaca aatcagcaat ttcaaatgga
    37801 aatgaaatta atcaagatat aaagatttca aatactccga aaaactcaag ccaaggtaac
    37861 aatctagttg ttaataacaa tgaacctact aaagaaatta aaattgcaaa cttggaagct
    37921 caaaattcta atcagaagaa aacgaataaa gttactaata attactttgg ttactacagt
    37981 tttagagaag ctccaaaaac acaaatctat actgtaaaaa aaggagacac acttagtgct
    38041 atagcgttaa aatacaaaac tacagtttca aatattcaaa atacaaataa tatagcaaat
    38101 cctaatttaa tatttattgg tcaaaaatta aaagtgccaa tgacaccatt agtagaacca
    38161 aaaccaaata cagtgtcttc aaataataaa agtaatagta atagcagtac attaaattat
    38221 ttgaaaacat tagagaatag aggatgggat ttcgacggta gttatggatg gcaatgtttc
    38281 gatttagtca atgtatattg gaatcatctt tatggtcatg gattaaaagg atatggagct
    38341 aaagatatac catatgcaaa taattttaat agtgaagcta aaatttatca caacacacca
    38401 actttcaaag ctgaacctgg ggacttagtg gtttttagtg gaagatttgg tggaggatat
    38461 ggtcatacag ctattgtttt aaatggtgat tatgatggaa aattaatgaa gttccaaagt
    38521 ttagatcaaa actggaataa tggtggatgg cgtaaatcag aggttgcaca taaagttgtt
    38581 cataattatg aaaatgatat gatttttatt agaccattta aaaaagcata atttaaatca
    38641 aaggcaggac attgaaatat gaaatttaca actttaagtg aagaagaatt taccaactac
    38701 accaaaaaac acttcaaaca ttatacacag tctatagaat tatataatta tagaaataaa
    38761 ataaattatg aagcacatat tgtgggagtg aagaatgata aaaatgaagt tatagctgca
    38821 tgtttattaa cagaagcacg tatttttaaa ttctacaaat atttctactc tcatagaggc
    38881 cctttacttg attatttcga tgctaaatta gtttgttact tttttaaaga attatctaaa
    38941 ttcatttata aaaatagagg agtatttatt cttgttgatc catatttaat agagaattta
    39001 agagatgcaa atggtagggt aataaagaat tataataatt cagtgatagt aaagatgcta
    39061 gggaaaattg ggtatctcca tcaaggttat acaacaggat attcaaataa aagtcaaatt
    39121 aggtggattt ctgtattgga tttaaaagat aaagatgaga atcaactttt aaaagaaatg
    39181 gaataccaaa ctagaagaaa tataaaaaag actattgaga ttggtgttaa ggttgaagat
    39241 ttatctattg aagaaacaaa tcgattttat aaattgtttc aaatggctga agaaaaacat
    39301 ggttttcatt tcatgaatga agattatttt aaacaaatgc aagaaatata taaaggtaag
    39361 gcaatgttaa agatagcttg tatagatctt aatgaatatc aagataaatt aaaaatacaa
    39421 ttattgaaaa tcgaaaatga aatgatgact gtgaacagat cattaaatga aaat
//
LOCUS       DAHHOJ010000013        37377 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.013, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000013 DAHHOJ010000000
VERSION     DAHHOJ010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 37377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.013"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..269)
                     /locus_tag="NBH32_000572"
     CDS             complement(<1..269)
                     /locus_tag="NBH32_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCD1514203.1"
                     /translation="MALTIRYQTTYEPFKVVDDIKEIPKDATIVWYDFDEPNEQENEW
                     FKAHFNFNDLEVDDAINGMPRAKYKSYKDYQYLVFHSIMGSNYSP"
     gene            366..857
                     /locus_tag="NBH32_000573"
     CDS             366..857
                     /locus_tag="NBH32_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCD1514204.1"
                     /translation="MVIIKQLFTHTQTVTSEFIDHNNHMHDANYNIIFSDVVNRFNYS
                     HGLSLKERENLAYTLFTLEEHTTYLSELSLGDVFTVTLYIYDYDYKRLHLFLTLTKED
                     GTLASTNEVMMMGINQHTRRSDAFPESFSTQIAHYYKNQPTITWPEQLGHKIAIPHKG
                     ALK"
     gene            854..1273
                     /locus_tag="NBH32_000574"
     CDS             854..1273
                     /locus_tag="NBH32_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514205.1"
                     /translation="MTDALQQKIHIELLDLLDDVKFELTELNAQKGLYINGPANQLLK
                     RGVHMAYVQGQKQAIDNIMTIVEQQLEDQHFLEHYDKFQNEVAHRNYDKTANFAELSD
                     IPRQFDNFLDQFYQIKGQYFIITHINTLIGDFHSEAH"
     gene            complement(1378..2298)
                     /locus_tag="NBH32_000575"
     CDS             complement(1378..2298)
                     /locus_tag="NBH32_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000620861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1514206.1"
                     /translation="MIRNRVMNSVVNKYLLHNRSIMFKNDQDVERFFYKRKIENRKKH
                     KQPSTLNVKANLEKLSLDDMQVFRFNFRHQIDKKILYIHGGFNALQPSPFHWRLLDKI
                     TLSTLYEVVLPIYPKTPEFHIDDTFQAIQRVYDQLVSEVGHQNVVVMGDGSGGALALS
                     FVQSLLDNQQPLPNKLYLISPILDATLSNKDISDALIEQDAVLSQFGVNEIMKKWANG
                     LPLTDKRISPINGTIEGLPPVYMFGGGREMTHPDMKLFEQMMLQHHQYIEFYDYPKMV
                     HDFPIYPIRQSHKAIKQIAKSIDEDVTQNN"
     gene            2506..3144
                     /locus_tag="NBH32_000576"
     CDS             2506..3144
                     /locus_tag="NBH32_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514207.1"
                     /translation="MTNPNQRLEPFDETFQQPNIHRGKRYGKKKRSLVSMIIQIIVVI
                     LTAIAGYSMWKQPIFNIVFTKESVDFDGIKNFNETIGKMHQLNINLGNINDLQQGIDR
                     LILVFNGFFILCIVSLVITILTIVFNRTVLKVVNILVLTIMLVITLYFSYIIKALAQE
                     IADVFKKYYVNVPPDQVITEADAIHNGIILLGCSIGLLVISLFFRNRMPRLK"
     gene            complement(3391..5328)
                     /locus_tag="NBH32_000577"
     CDS             complement(3391..5328)
                     /locus_tag="NBH32_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCD1514208.1"
                     /translation="MTTTFIISYIVLALIIVGVINLLLIRSRRKAKRQQKEQHFATRQ
                     SNQSKFKASDLDRTTDQSSQHTAREEARIDNQDNQNQVSLNKQTEGSEQNQASFTDNN
                     ESDEEVYAAKNPKSEEYKVNEKIKKEHKNFIFGEGVSRGKILAALLFGMFIAILNQTL
                     LNVALPKINTEFNISASTGQWLMTGFMLVNGILIPITAYLFNKYSYRKLFLVALVLFT
                     IGSLICAISMNFPIMMVGRVLQAIGAGVLMPLGSIVIITIYPPEKRGAAMGTMGIAMI
                     LAPAIGPTLSGYIVQNYHWNVMFYGMFIIGIIAILVGFVWFKLYQYTTNPKADIPGII
                     FSTIGFGALLYGFSEAGNKGWGSVEIETMFAIGIIFIILFVIRELRMKAPMLNLEVLK
                     FPTFTLTTVINMVVMMSLYGGMILLPMYLQNLRGFSALDSGLLLLPGSLIMGLLGPFA
                     GKLLDTIGLKPLAIFGIAVMTYATWELTKLNMDTPYMTIMGIYVLRSFGMAFIMMPMV
                     TAAINALPGRLASHGNAFLNTMRQLAGSIGTAILVTVMTTQTTQHLSAFGEELDKTNP
                     VVQDHMRELASQYGGQEGAMKVLLQFVNKLSTVEGINDAFIVATIFSVIALILCLFLQ
                     SNKKAKATAQKIDADNSINHE"
     gene            complement(5341..5988)
                     /locus_tag="NBH32_000578"
     CDS             complement(5341..5988)
                     /locus_tag="NBH32_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCD1514209.1"
                     /translation="MKKMILINVITVVVLLAIGIAGFYFWNKTTSYITTDNAKVNGDQ
                     IKIASPASGQIKSLNVKQGDKLDKGDKVATVTVQGQDGETKDMELKMPQKGTIAKLDG
                     MEGSMVQAGNPIAYAYNLDDLYVTANIDEKDIKDVEVGKDVDVTIDGQKASVKGKVDS
                     IGKATAASFSLMPSSNSDGNYTKVSQVIPVKITLDSEPSKQVVPGMNAEVKIHKN"
     gene            6109..6663
                     /locus_tag="NBH32_000579"
     CDS             6109..6663
                     /locus_tag="NBH32_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1514210.1"
                     /translation="MKRQAKIEIQNALVDLMAEYPFQEISTKMICAYCNINRSTFYDY
                     YKDKFDLLDTINSKHKEKFQFLLSALHHNFENIKQDKLKLYKFFIIIAKYIKRNEQFF
                     KDILVTYPMKTLFIDYINLARDYYQQIMNDYSTTVTNKQLYVTYIIGGQAGVFINWLS
                     NGCNESPEEVADILLANTIKLQNQ"
     gene            complement(6890..8098)
                     /locus_tag="NBH32_000580"
     CDS             complement(6890..8098)
                     /locus_tag="NBH32_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HCD1514211.1"
                     /translation="MNDLKRFNKRSPIFIIILGSLTAIGALSIDMFLPGLPDIRHDFQ
                     TTTSNAQLTLSMFMIGLAFGNLFAGPISDSNGRRKPLIIAMIIFTLASLGIVFVHNIW
                     LMIALRFLQGVTGGAAAVISRAIASDMYSGNELTKFMALLMLVNGIAPVVAPTIGGII
                     LNYSVWRMVFVILTIFGFVMVIGSLLKVPESLTVTNRESSSGLKTMFKNFKILLKTPR
                     FVLPMLIQGMTFVILFTYISASPFIIQKIYGMTAIQFSWMFAGIGITLIISSQLTGYL
                     VDFIDSQKLMRVMTMIQIIGVILVTIVLLNHWNFWILAIGFIILIAPVTGVATLGFTI
                     AMDESSSGRGSSSSLLGLVQFLFGGIASPLVGVKGEDNPIPYIIIIIATAVILIILQI
                     YNMKVFKTNR"
     gene            complement(8208..8335)
                     /locus_tag="NBH32_000581"
                     /pseudo
     CDS             complement(8208..8335)
                     /locus_tag="NBH32_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501095.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(8366..9748)
                     /locus_tag="NBH32_000582"
     CDS             complement(8366..9748)
                     /locus_tag="NBH32_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCD1514212.1"
                     /translation="MKSCPKCGQQAQDDVQICTQCGHKFDSRQALYRKSTDEDIQTNN
                     IKMRKMVPWAIGFFILILIIILFFLLRNFNSPEAQTKILVNAIENNDKQKVATLLSTK
                     DNKVDSEEAKVYINYIKDEVGLKQFVSDLKNTVHKLNKSKTSVASYIQTRSGQNILRV
                     SKNGTRYIFFDNMSFTAPTKQPIVKPKEKTKYEFKSGGKKKMVIAEANKVTPIGNFIP
                     GTYRIPAMKSTENGDFAGHLKFDFRQSNSETVDVTEDFEEANITVTLKGDTKLNDSSK
                     KVTINDREMAFSSFKTYGPYPQNKDITISASGKAKDKTFTTQTKTIKASDLKYNTEIT
                     LNFDSEDIEDYVEKKEKEENSLKNKLIEFFAGYSLANNAAFNQSDFDFVSSYIKKGSS
                     FYDDVKKRVSKGSLMMISSPQIIDAEKHGDKITATVRLINENGKQVDKEYELEQGSQD
                     RLQLIKTSEK"
     gene            complement(9988..10443)
                     /locus_tag="NBH32_000583"
     CDS             complement(9988..10443)
                     /locus_tag="NBH32_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1514213.1"
                     /translation="MVKHLQDHIQFLEQFINNVNALTAKMLKDLQNEYEISLEQSNVL
                     GMLNKEPLTISEITQRQGVNKAAVSRRIKKLIDAKLVKLDKPNLNIDQRLKFITLTDK
                     GRAYLKERNAIMTDIAQDITNDLNSEDIENVRQVLEVINHRIKTYSNHK"
     gene            10638..11606
                     /locus_tag="NBH32_000584"
     CDS             10638..11606
                     /locus_tag="NBH32_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HCD1514214.1"
                     /translation="MLLNLLLLFILIVICPLIFNYTLTLNLTCYILVVISIFLSLIYS
                     IMTYTFPWSMFITFIIISIIMLFKHRYLFSHTSSRLFIKKLLSFSYKFVLYTSALLFI
                     STIQSPIVKGLSMWLAILCLSLLLTFICYLVWTVSYRHRSYNKEVDIIIVLGAGIFTE
                     FVTPMLAARLDRALDIYQQQVSATKIIVSGGQGPDEPIPEALAMQRYLIAHGVDKTDI
                     LMESHSINTYTNFLYSKQIINNLYHEAVKIVCVTSQFHVLRALRLAQKLGLSIDGVGS
                     HTPYHFFLHVLIKDYLGVMYQYRLLLTLYCSSSWFICLYAAFIYNS"
     gene            complement(11837..12502)
                     /locus_tag="NBH32_000585"
     CDS             complement(11837..12502)
                     /locus_tag="NBH32_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514215.1"
                     /translation="MALVVKDIVKNFGEGLSETKVLKGINFEVEQGEFVILNGASGSG
                     KTTLLTILGGLLSQTSGTVLYNDAPLFDKQHRPSDLRLEDIGFIFQSSHLVPYLKVIE
                     QLTLVGQEAGMTKQQSSTRAIQLLENIGLEDRLNVYPHQLSGGEKQRVAIMRAFMNNP
                     KIILADEPTASLDADRATKVVEMIRQQIKEQQMIGIMITHDRRLFEYADRVIELEDGK
                     ITD"
     gene            complement(12502..13557)
                     /locus_tag="NBH32_000586"
     CDS             complement(12502..13557)
                     /locus_tag="NBH32_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1514216.1"
                     /translation="MKLAIKEIMFYKFRYILITLIILLLSIMVLFISGLAQGLGRENI
                     SLFEHFDNDEYVVQKMKEPQIEKSQLSDTQQNQIKKVIHQEPYKMNIQTLKLSNKEQD
                     MITMNDVKQQRIQLKKGDYPKNAHEVAINDKLAADNIRVGDRLHFKNNSTSYRVSGIL
                     NDTMYAHSSIVLLNDNGFNALNKVNTAFYPVKNLTQQQRDELNKINDVQVVSEKDLTG
                     NIASYQAEQAPLNMMIVSLFAITAIVLSAFFYVMTIQKISQIGILKAIGIKTRHLLSA
                     LVLQILTLTIIGVGIAVIIIVGLSFMMPVTMPFYLTTQNILLMVGIFILVAILGASLS
                     FVKLFKVDPIEAIGGAE"
     gene            13693..14367
                     /gene="hssR"
                     /locus_tag="NBH32_000587"
     CDS             13693..14367
                     /gene="hssR"
                     /locus_tag="NBH32_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding heme response regulator HssR"
                     /protein_id="HCD1514217.1"
                     /translation="MVQCLVVDDDPRILNYIASHLQTEHIDAYTQPSGEAALKLLEKQ
                     RVDIAVVDIMMDGMDGFQLCNTLKNDYDIPVIMLTARDALSDKERAFISGTDDYVTKP
                     FEVKELIFRIRAVLRRYNINSNSEMTIGNLTLNQSYLELQVSNKTMTLPNKEFQLLFM
                     LAARPKQIFTREQIIEKIWGYDYEGDERTVDVHIKRLRQRLKKLNATLTIETVRGQGY
                     KVENHV"
     gene            14360..15733
                     /gene="hssS"
                     /locus_tag="NBH32_000588"
     CDS             14360..15733
                     /gene="hssS"
                     /locus_tag="NBH32_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000477337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme sensor histidine kinase HssS"
                     /protein_id="HCD1514218.1"
                     /translation="MFKTLYARIAIYSITVILFSALISFVLTNVYYHYNLKASNDAKI
                     MKTLKEARQYEQSAKPTHIQQYFKHLGQMNYQIMTVDQKGHKTFYGEPFREDTLSQNA
                     INNVLNNKDYHGIKDKPFALFVTGFFDNVTDNTVGINFKTKDGSIAVFMRPDIGETFS
                     EFRTFLAVLLMLLLFISISLVIASTYSIIRPVKKLKLATERLIDGDFETPIKQTRKDE
                     IGTLQYHFNKMRESLRQVDQMRQHFVQNVSHEIKTPLTHIHHLLSELQQTSDKTLRQQ
                     YINDIYTITTQLSGLTTELLLLSELDNHQHLLFDDKIQVDQLIKDIIRHEQFSADEKS
                     LIILADLESIDFLGNQRLLHQALSNLLINAIKYTDVGGAIDIALQHSHNNIIFTISND
                     GSPISPQAEARLFERFYKVSKHDNSNGLGLAITKSIIELHHGTIQFTQSNEYVTTFTI
                     TLPNNSH"
     gene            15896..16339
                     /locus_tag="NBH32_000589"
     CDS             15896..16339
                     /locus_tag="NBH32_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1514219.1"
                     /translation="MMKLNLFINATETESYIDIHAPKMNDHVQSIINAVNDLDKSHTL
                     VGYIDKEIHIINVSDVITFQVINKNVTAITSNQKFKLKLRLYELEKQLPQHFIRISKS
                     EIVNKYYIEKLLLEPNGLIRMYLKDAHYTYSSRRYLKSIKERLSI"
     gene            16336..16791
                     /locus_tag="NBH32_000590"
     CDS             16336..16791
                     /locus_tag="NBH32_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HCD1514220.1"
                     /translation="MKNLKNSLFISLIIGLSLSLFFSMLFADGKYYPLNPQSTIGILY
                     YNHFTETTVMLISIILWLLIGVVFFLGDFIFKYTDWGITKATIVHFITTYVGFLPLAI
                     LAGWFPLTINSLIIFTIIFIVVYTLIWIIQFFKNKNYVDTINEQLKQLK"
     gene            complement(16968..17531)
                     /locus_tag="NBH32_000591"
     CDS             complement(16968..17531)
                     /locus_tag="NBH32_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000372852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HCD1514221.1"
                     /translation="MDQTAFTDSRVTKTINRIHDGMVNLLKMKAYNDITVKDICEKSQ
                     ISRTTFYTHYKTKDDFIYSYLNTLLKVAKKKFIKERPMTQAVFFKRMIYYWLSEGQLI
                     LMLLGDESAYKLHQVIKKSLQQRIEINVVPVLNTKMLTTKEKYFLLIFMSNAIIGVLQ
                     DWVKRGYKESPKEVAEIMNKIFENAFR"
     gene            17665..18393
                     /locus_tag="NBH32_000592"
     CDS             17665..18393
                     /locus_tag="NBH32_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000388431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514222.1"
                     /translation="MDATIAKLDKVVKTYNKHHVLNEISMSINKGELLGLIGPSGSGK
                     TTTIKCLLGMEKVDSGSAEIFAHKMPNRKALNRLGYMGQTDALYENLTAYENLEFYGR
                     LSGLKGNVLKDNIEKNIVLVNLEQALNKKVAQFSGGMKRRLSLAITLLAEPELIILDE
                     PTVGIDPKLRQQIWQQFKQMTKDGKSVVITTHVMDEAERCDKVGLIVDGRIFAIDTPT
                     NLKQRFNVDTIEAVFIKAEEVRQS"
     gene            18390..19472
                     /locus_tag="NBH32_000593"
     CDS             18390..19472
                     /locus_tag="NBH32_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000696075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1514223.1"
                     /translation="MKILAIVKRVLKEMLRDKRTLALMFLAPILILTLMYFIFNGDEN
                     DLKIGIDTSVSSNITKQLPSSDIKTQTFHSHENIRSKIENNHLDGYIYQENQTLHVIY
                     TNEDPNKSNMIKQMIGKAIQQDKISSIKHLIEKAPPTMKAPNKQDIKISHSYLYGNSD
                     STYFDKMFPILMGFFVFLFVFLISGIALLRERTTGTLERVLSTSIRRSEIVLGYLIGY
                     GIFAIIQTLIIVLFSIYLLNINLAGSLWYVILINICLAITALSMGIFISTFANSEFQM
                     IQFIPLVAVPQVFFSGIFPLENMPDWLGNIGYLFPLRYAGDALTNIMIKGQGWSNIWF
                     DLLILMIFIIVFIILNIVGLKRYRQV"
     gene            complement(19599..21077)
                     /gene="mqo"
                     /locus_tag="NBH32_000594"
     CDS             complement(19599..21077)
                     /gene="mqo"
                     /locus_tag="NBH32_000594"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD1514224.1"
                     /translation="MTTQHSKTDVILIGGGIMSATLGTLLKELSPEKNIKVFEKLAQP
                     GEESSNVWNNAGTGHSALCELNYTKEGKDGTVDCSKAIKINEQYQISKQFWAYLVKTG
                     QLDNPDRFIQAVPHMSFVIGEDNVAFIKSRVATLKKSVLFEKMKLSQDEEEMKSWVPL
                     MIEGRKSDEPIALTYDETGTDVNFGALTAKLFDNLEQRGVEIQYKQNVLDIKKQKSGA
                     WLVKVKDLETNETTTYESDFVFIGAGGASLPLLQKTGIKQSKHIGGFPVSGLFLRCTN
                     QEVIDRHHAKVYGKAAVGAPPMSVPHLDTRFVDGKRSLLFGPFAGFSPKFLKTGSHMD
                     LIKSVKPNNIVTMLSAGIKEMSLTKYLVSQLMLSNDERMDDLRVFFPNAKNEDWEVIT
                     AGQRVQVIKDTEDSKGNLQFGTEVITSDDGTLAALLGASPGASTAVDIMFDVLQRCYR
                     DEFKGWEPKIKEMVPSFGYRLTDHEDLYHKINEEVTKYLQVK"
     gene            complement(21458..23056)
                     /locus_tag="NBH32_000595"
     CDS             complement(21458..23056)
                     /locus_tag="NBH32_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1514225.1"
                     /translation="MLVNTFNPFDNLLLSSLIAAIPIVLFLLCLTVFKMKGIYAAITT
                     LVVTLLIAIPFFKLPVGIASGAVVEGFFQGIIPIGYIVMMAVLLYKITVESGQFLTIQ
                     DSITNISQDQRIQVLLIGFAFNAFLEGAAGFGVPIAICALLLTQLGFNPLKAAMLCLV
                     ANAASGAFGAIGIPVGVVETLKLPGDVSVLGVSQSATLTLAIINFIIPFLLIFIIDGF
                     RGVKETLPAILVVSITYTLTQGLLTVFSGPELADIIPPLLTMLALAVFSKKFQPKHIY
                     RVNKDEEIEPAKAHSAKAVLHAWSPFIVLTVIVMIWSAPFFKNLFLPNGALSSLVFKY
                     NLPGTVSEVTHKPLVLTLNIIGQTGTAILLTIIITILMSKKVNFKDAGRLFGVTFKEL
                     WLPVLTICFILAISKITTYGGLSAAMGQGIAKAGNVFPVLSPILGWIGVFMTGSVVNN
                     NSLFAPIQASVAQQIGTSGSLLVSANTVGGVAAKLISPQSIAIATAAVKQVGKESELL
                     KMTLKYSVCLLIFICIWTFILSLL"
     gene            23373..25046
                     /locus_tag="NBH32_000596"
     CDS             23373..25046
                     /locus_tag="NBH32_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1514226.1"
                     /translation="MIKQINVSNMQKFESQLMKAQSEGYTHVVPYANEIMIYQSMLDA
                     VQLYPKSIVVDYTVDGQYKNDCHYFGQSTINIADWAQNNNYYPNLIYAIQQTLDLIHY
                     YSVETIFDLALLTLLKGDLSIDGHVVFDFKAPLATSASIWETIKTIEDFDMMSQFYLN
                     KMAYIDHHPIPFRNLFIEDSEQLNSPDNWLYSTKFMLPKWLYKIAKQRADNKQLQNFG
                     LYTKQPNVLKDHIVFIGDHHQYIGNSKYLFTYFVKHNPMTACYFVTDDRRGPHFISPK
                     SEKADELINSARVVLVENDIPETLQPNGTLIQLHQGTPIMQLFLDSKEPIKNIETPFY
                     RAKRYNRWLQFDYVIHSADDISHFYQTAFPSHQANVLAYGNPKHQYLLQKRNESTTQQ
                     QYKKSFKINDQKPVLLYAPIGLVSAQQLPLSDALFKAYHVVVQGIDETMLPEEAIVAP
                     KYLSAQDLILMSDVVITDYSNIIFDAMAIDKSVALYTPNHSQYIESQGVNEDIWRHLS
                     KIWYTDRQLLINNLISQAIPVIKYPQIQHKEQPLESISQLILSKMTSNQ"
     gene            complement(25201..25830)
                     /locus_tag="NBH32_000597"
     CDS             complement(25201..25830)
                     /locus_tag="NBH32_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514227.1"
                     /translation="MKRLVTGLLALSLFLAACGQDSDQQKDSNKEKDDKAKTEQQDKK
                     TNDSSKDKKDNKDDSNDVNKDNKDNSANDNQQQSNSNATNNDQNQTNNNQSSNNQANN
                     NQKSSYVAPYYGQNAAPVARQIYPFNGNKTQALQQLPNFQTALNAANNEANKFGSNNK
                     VYNDYSIEEHNGNYKYVFSFKDPNANGKYSIVTVDYTGQAMVTDPNYQQ"
     gene            complement(26144..26659)
                     /locus_tag="NBH32_000598"
     CDS             complement(26144..26659)
                     /locus_tag="NBH32_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1514228.1"
                     /translation="MAGIIKEISKQDAGELVELATRTFYDTFGSYYDDKDFDQFFKDN
                     YTVEKFTQEINHVDSFHYFYQEDGANVGYIKMNINSAQTEEMGETYLEVQRIYFLKDF
                     QGGGRGSQLIELAEKIAQEHNKHKIWLGVWEHNPRAQAFYKRHGFKVVGEHHFQTGDV
                     TDTDLIMEKEL"
     gene            26850..27854
                     /locus_tag="NBH32_000599"
     CDS             26850..27854
                     /locus_tag="NBH32_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000573244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1514229.1"
                     /translation="MIETFKAFVIDKDESGKVTPTFKQLSPTDLPKGDVLIKVHYSGI
                     NYKDALATQDHNAVVKSYPMIPGIDLAGTIVESEAPGFEKGEQVIVTSYDLGVSHYGG
                     FSEYARVKSEWIIKLPDTLTLEESMIYGTAGYTAGLAIERLEKVGMNIEDGPVLVRGA
                     SGGVGTLAVLMLNELGYKVIASTGKQDVSDQLLELGAKEVIDRLPVEDDHKKPLASST
                     WQACVDPVGGEGINYVTKRLNHSGSIAVIGMTAGNTYTNSVFPHILRGVNILGIDSVF
                     TAMKLRQRVWRRLAKDLMPENLHEIKQVITFDELPEQLNKVIKHENKGRIVIDFGVDK
                     "
     gene            complement(28036..28212)
                     /locus_tag="NBH32_000600"
     CDS             complement(28036..28212)
                     /locus_tag="NBH32_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000687295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514230.1"
                     /translation="MKIDTLRQAIIYYFVALVLSALMIYLFLTNIERVILYSCAFTVV
                     YWAIYAFDVYRKRN"
     gene            complement(28377..28778)
                     /locus_tag="NBH32_000601"
     CDS             complement(28377..28778)
                     /locus_tag="NBH32_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000241591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1514231.1"
                     /translation="MVKVTYDIPTCEDYCALRINAGMSPKTREAAEKGLPNALFTVTL
                     YDEDRLIGMGRVIGDGGTVFQIVDIAVLKSYQGQGYGSQIMEHIMKYIKNVSVESAYV
                     SLIADYPADKLYTKFGFMPTEPDSGGMYIKF"
     gene            complement(28814..29848)
                     /locus_tag="NBH32_000602"
     CDS             complement(28814..29848)
                     /locus_tag="NBH32_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1514232.1"
                     /translation="MKDVTIIGGGPSGLYASFYAGLRDMSVRLIDVQSELGGKMRIYP
                     EKIIWDIGGIAPKPCHEILKDTIKQGLYFKPEVHLNERVVDIRKKAERHFEVETEAGE
                     IYTSKAVIIAIGAGIINPKQLDVKGVERYQLTNLHYVVQSYRRFKDKDVLISGGGNTA
                     LDWAHDIAKIAKSVTVVYRKEDVSGHEAMKTLVTDLNVKLCPKTRIKYLVGNDDETHI
                     SEVVLEHVESGDTHTVKFDDVIISHGFDRCNTLLSETSSKLDMHDDCRVKGFGNTTTS
                     IPGIYACGDIVYHDAKSHLIASAFSDGANAANLAKTYIQPDANAEGYVSSHHEVFKEA
                     NKTIVNKHLY"
     gene            complement(29859..29975)
                     /locus_tag="NBH32_000603"
     CDS             complement(29859..29975)
                     /locus_tag="NBH32_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514233.1"
                     /translation="MANYAIYFNNININSIMSLNDNNYQFERVNERNEYRES"
     gene            complement(30080..30511)
                     /locus_tag="NBH32_000604"
     CDS             complement(30080..30511)
                     /locus_tag="NBH32_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2871 domain-containing protein"
                     /protein_id="HCD1514234.1"
                     /translation="MRRLLYSFLFYMIIGLFSGFFYRELTKAYDFTGTTQLSLVHTHT
                     LILGMFMFLILLPLEKLFKLSSYYLFNWFFYVYNIGVIVTIGMMVTKGFFQVTGKSYS
                     PEAFAGFAGIGHTGMLAGLLLLFFLLRQAILKEPRDESFKK"
     gene            complement(30717..31994)
                     /locus_tag="NBH32_000605"
     CDS             complement(30717..31994)
                     /locus_tag="NBH32_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD1514235.1"
                     /translation="MNIFQNKLLWIAPIATMIILVIFSLAFYPAYNPKPKDLPIGILN
                     EDKGTTIQDKNVNIGKKLEDKLLDSDSNKIKWVKVDSEKDLEKDLKDQKIFGVAIIDK
                     DFSKDAMSKTQKVVMDSKKEEMQQKVASGEIPPQVVQQMKQKMGNQQVEVKQAKFKTI
                     VSEGSSLQGSQIASAVLTGMGDNINAQITKQSLETLTSQNVKVNAADINGLTNPVKVD
                     NEKLNKVKDHQAGGNAPFLMFMPIWIGSIVTSILLFFAFRTSNNIVVQHRIIASIGQM
                     IFAVVAAFAGSFVYIYFMQGVQGFDFDHPNRIAIFVALAILGFVGLILGVMVWLGMKS
                     IPIFFILMFFSMQLVTLPKQMLPESYQKYVYDWNPFTHYATSVRELLYLNHQIELNST
                     MWMFIGFMIFGAVSSLVSAIVRKHSTKRTEVPS"
     gene            32155..32778
                     /locus_tag="NBH32_000606"
     CDS             32155..32778
                     /locus_tag="NBH32_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1514236.1"
                     /translation="MKEDRRIRKTKSSIKQAFTKLLQEKDLEKITIRDITTRADINRG
                     TFYLHYEDKYMLLADMEDEYISELTTYTQFDLLRGSSIEDIANTFVNDILKNIFQHIH
                     DNLEFYHTILQLERTSQLELKINEHIKNNMQRYISINHSIGGIPEMYFYSYVSGATIS
                     IIKYWVMDKQPISVDELAKHVHNIVFNGPLRIMAENRLHKSNLDSLT"
     gene            32824..33768
                     /locus_tag="NBH32_000607"
     CDS             32824..33768
                     /locus_tag="NBH32_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HCD1514237.1"
                     /translation="MITSFRHSEDIDKHIIKTPLDHTASWINVVEPDREEIENLMEQY
                     NIPEDFIRDPLDSEESSRIEYDEDTGYSLIIIDLPIVNSTNRSVLSFVTIPLGIIIGN
                     GIIVTVCDAENEFLENLPKRDINLKFHSRFALEILTTIADHYNRNLRLLNKSRIRIEK
                     ELKNNITNKQLFKLMEVEKSLVYFLAALKGNDTIIKKLFRLPAIKRFEEDEELLEDLI
                     IENNQAIETTELHQRILESITTSYASLLSNDMNTIMKTLTLFTVLLTLPTLVFSFFGM
                     NVPLPIDDHSYISWIIVVGISLILVVIVSIFLWRKQKL"
     gene            complement(33854..35296)
                     /locus_tag="NBH32_000608"
     CDS             complement(33854..35296)
                     /locus_tag="NBH32_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sucrose transporter subunit IIBC"
                     /protein_id="HCD1514238.1"
                     /translation="MNYKQSAEDILNAIGGEENLDAMAHCATRLRLVLNDESLVNEEA
                     LNNMDVVKGTFSTGGQYQIIIGSGTVNKVFSELEKLTGKEASTTSEVKAQSAKNMNPL
                     QRFVKMLSDIFVPIIPAIVAGGLLMGLNNILTAKDLFFSGKSLIDVYSQFAGLAEMIN
                     VFANAPFTLLPILIGFSAAKRFGGNPFLGAALGMILVHPSLMSAYDFPKAVEAGKAIP
                     YWDVFGLHINQVGYQGQVLPMLVAAYILASIEKGLRKVIPTVLDNLLTPLLSIFITAF
                     LTFSFVGPITRQLGYWLSDGLTWLYEFGGAIGGLIFGLLYAPIVITGMHHSFIAVETT
                     LIADATKTGGSFIFPIATMSNVAQGGAAIAAFFIIKQNKKLKGVASAAGISALLGITE
                     PAMFGVNLKLRYPFIGAIVGSGIGSAYIAFFKVKAIALGTAGLPGFISINPVHAGWLH
                     YFVGMTISFIVAITVTLILSKRKANKEVVE"
     gene            35479..35862
                     /locus_tag="NBH32_000609"
     CDS             35479..35862
                     /locus_tag="NBH32_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCD1514239.1"
                     /translation="MKERGHIEQLWREEKYHVLLHSQQSYQMIRNALKTDLSLHQVQQ
                     MIDDALLIEPSIGSVCNAFDHMWGYFKKCANEEERQQSKLLKADFINGKIDTQTLLDF
                     LAELANKYDVQYLLQSRVLNTKRKR"
BASE COUNT        12563 a         6504 c         5278 g        13032 t
ORIGIN      
        1 attggtgaat agtttgaccc cataatacta tgaaatacta agtattgata atctttataa
       61 gatttatatt tagctcgtgg cataccgtta attgcatcat ccacttctaa atcattaaaa
      121 ttaaaatgtg ctttaaacca ttcattttct tgttcattcg gttcatcaaa atcataccaa
      181 acaatagtcg catcttttgg tatctctttg atatcatcaa ctactttaaa cggttcatat
      241 gtagtttgat accgtatcgt taaagccatc gatactcccc ctaaataacg aattctctat
      301 tattttatca tgaattaaat aacgtgtatg tcttaattta ttttagtatg atagtcacta
      361 aggagatggt tattatcaaa caacttttta cacatactca aaccgtaaca tctgaattca
      421 ttgaccataa caatcatatg catgatgcaa attataatat catttttagt gacgtcgtga
      481 atcgttttaa ttacagccac ggtctttctt taaaagaacg cgaaaattta gcatatacgc
      541 tatttacact agaagaacat acgacatacc tctcagaatt gtctcttggc gatgtattta
      601 ctgttacttt atatatttat gattacgatt ataagcggct gcatttattt ttaacattaa
      661 ctaaagaaga tggtacacta gcatcaacaa atgaagttat gatgatggga attaatcagc
      721 acacacgtcg ttctgatgct tttcctgaat cattttcaac acaaatagca cactattata
      781 aaaatcaacc aactatcact tggcctgaac aattagggca taaaatagca attccacaca
      841 aaggagcatt aaaatgacag atgcattaca acaaaagatt catatcgaat tactagattt
      901 attagatgat gttaagtttg aattaacaga attaaatgca caaaaagggt tatacattaa
      961 cggaccagca aatcagctac ttaagcgtgg cgtgcatatg gcttatgttc aaggacaaaa
     1021 gcaagccatc gataatatta tgactattgt ggaacagcag cttgaagatc aacatttcct
     1081 agaacattat gataaatttc aaaatgaggt tgctcatcgc aattatgata aaacagctaa
     1141 ctttgcagaa cttagtgata ttccacgtca attcgataat tttttagatc aattttatca
     1201 aattaaaggg caatacttta tcatcacaca tatcaataca cttattggtg attttcactc
     1261 agaagctcat taacaattag tctatataac ccttgctata ttttcaaaaa aaaccaatta
     1321 cgttttcatg tcaaatatca tcttgcatga aatcgtaact ggatcaattg tatgttatta
     1381 gttattttgt gttacatcct catctatcga tttggcaatt tgtttaatag ctttatgtga
     1441 ttgtctaatt ggataaattg gaaaatcatg taccatctta ggataatcat aaaactcaat
     1501 gtattgatga tgttgcaaca tcatttgttc aaatagcttc atatcaggat gtgtcatttc
     1561 acgtccacca ccaaacatat aaactggtgg caatccttct attgtgccat taattggcga
     1621 tatgcgctta tctgttaatg gtaggccatt cgcccatttt ttcataatct cattgacacc
     1681 aaactgactt agaaccgcat cttgttcgat taaggcgtcc gaaatatctt tattagatag
     1741 tgttgcatct aaaattggtg agattaaata caatttattc ggtaatggct gttgattatc
     1801 taaaagagat tgtacaaagg ataatgccag tgcaccacct gaaccatcac ccatgactac
     1861 gacattttga tgtcctactt cagatactaa ttgatcataa acacgttgta tcgcttggaa
     1921 agtatcgtca atatgaaact ctggtgtctt tggatagata ggcagtacaa cctcatataa
     1981 tgtacttaaa gtgattttat ccaacaatct ccaatggaac ggtgatggtt gtagtgcatt
     2041 gaatccaccg tgaatatata aaattttctt atcaatttga tgtctgaaat taaagcgaaa
     2101 gacttgcata tcatctaatg ataacttttc taaatttgct ttaacattta atgttgaagg
     2161 ctgcttatgt ttttttctat tttcaatttt tcttttataa aaaaatcttt caacatcttg
     2221 atcattttta aacataatcg agcgattgtg aagcaaatat ttattgacaa cgctattcat
     2281 aacacggttt ctaatcaatg tcttaaccta cctttatata ttttatgtat ccaatgattg
     2341 tctatcccct acattcttcg ccaaaaaaag tatataatat agaagatatt ttctttttca
     2401 ctttcaaatt taagactaca attgaacagt gatttttcat cattataaca gacaactaga
     2461 catattgata agtaaagaaa agaactttat acggaggtgc cttgcatgac aaatccaaat
     2521 caacgattag aaccatttga tgagacattt caacaaccga atattcatcg tggtaagcga
     2581 tatggtaaga aaaaacgttc attggtaagc atgattattc aaatcatagt tgttatatta
     2641 accgcaatcg ctggatacag catgtggaaa caacctattt ttaatattgt cttcaccaaa
     2701 gaatctgtag attttgatgg cattaaaaat tttaacgaga caattggaaa aatgcatcaa
     2761 ttaaatatta atttgggtaa tatcaatgat ttacaacaag gaatcgatcg cttaatttta
     2821 gtctttaacg gtttctttat tttatgtatc gttagcctag tcataacaat actgacaatt
     2881 gtatttaatc gcacagttct aaaagtagta aatattttag tgttaacaat tatgttagtc
     2941 attacattgt atttcagtta tattattaaa gcactcgcac aagaaattgc agatgttttt
     3001 aaaaaatatt atgtaaatgt accaccagat caagtgataa cagaagctga cgctattcat
     3061 aacggcatca ttttactggg ctgtagcatc ggtttacttg ttataagctt attcttccgt
     3121 aatcgcatgc ctcgtttaaa ataatgatat acagtaggga ttgggacaga aatgttattt
     3181 tcacaaaatt catttcgttg acccacttcc tgctttttct ttattaatga cctactacgt
     3241 caatgccaaa ttttaatata attcctctaa cattctacaa atcaacaaat aaaaaaagta
     3301 tcgataacca tatattggca ccgatacttt aaaaatcatg tccccacaac ataatttttg
     3361 attagtcaca cttcaattaa tttattttat ttattcatga ttgatactat tatctgcatc
     3421 tatcttttga gccgtagctt tggctttttt gttgctttgt aaaaataaac ataaaatcaa
     3481 ggcgatgacg ctaaatatcg ttgcaactat aaatgcatca ttaattcctt caactgttga
     3541 tagtttattt acaaattgta ataacacttt cattgctcct tcttgaccac catattgcga
     3601 tgctaattca cgcatatgat cttgtacaac aggattcgtt ttatctaact cttccccaaa
     3661 agctgataaa tgttgtgtag tttgtgttgt cattacagta accaagattg ctgttcctat
     3721 agaaccagct agttgacgca tcgtatttaa gaaggcatta ccatgggagg caagtcgtcc
     3781 gggcaacgca ttaatagctg ctgttaccat tggcatcata ataaatgcca taccaaatga
     3841 acgtagaaca tagatgccca tgattgtcat atatggcgta tccatattca atttagttaa
     3901 ttcccacgtt gcgtaagtca tgacagcaat accaaaaatg gctaaaggtt ttaaaccaat
     3961 cgtgtctaac aacttaccag caaatggtcc tagtagaccc ataattagag aaccaggtaa
     4021 taataacaat ccggaatcta atgctgagaa accacgtaaa ttttgtaagt acattggtaa
     4081 taaaatcata ccaccataca aactcatcat tacaaccata ttaattactg ttgttaatgt
     4141 aaatgttgga aatttcaata cttctaaatt caacattggc gctttcattc ttaattctct
     4201 tattacaaat agaataataa agataatacc aatcgcaaac attgtttcta tctcaacaga
     4261 accccagcct ttgttccccg cttctgaaaa gccgtataac aaagcaccga aaccaatcgt
     4321 gctaaaaatg atacctggga tatcagcttt agggtttgta gtatattgat ataacttaaa
     4381 ccatacaaaa cctactaaga ttgcaataat tccgataatg aacataccgt aaaacatcac
     4441 attccaatgg taattttgta caatataacc tgataatgtt ggaccgattg caggtgctaa
     4501 aatcattgcg atacccattg tacccatggc agcaccacgt ttttcaggtg gataaattgt
     4561 aataataaca attgaaccta atggcattag tacacctgca ccaatggctt gtaatacacg
     4621 tccaaccatc atgattggga aattcattga aatcgcacat attaatgaac caattgtaaa
     4681 gagtactaac gcaactaaaa ataattttcg atatgaatat ttattaaata gatacgccgt
     4741 aattggtatt aaaataccgt ttactaacat gaatcccgtc atcaaccatt gtcctgttga
     4801 cgcagaaata ttaaattccg tattaatttt tggcaaagca acatttaata atgtttggtt
     4861 taaaatcgca ataaacatac cgaataataa tgccgctaat attttaccgc gtgaaacacc
     4921 ttcaccaaaa ataaagtttt tatgttcttt ttttattttt tcatttactt tatattcttc
     4981 tgatttagga tttttagcag catatacttc ttcatcagat tcattattat cagtgaatga
     5041 tgcttgattt tgttcagatc cctctgtctg tttgtttaga ctaacttgat tctgattatc
     5101 ttgattgtca atacgtgcct cttcacgcgc cgtatgttgt gacgattgat cagttgttct
     5161 gtctaaatca cttgctttaa atttagattg atttgattga cgtgtcgcaa aatgttgttc
     5221 cttttgttgg cgttttgctt ttcttcttga tcttattaac aataaattga taaccccaac
     5281 aataatgagc gctaaaacaa tgtagctaat aatgaaggtc gtagtcattt aatgaccccc
     5341 ttaattttta tgaattttta cttcagcgtt cattccagga acaacttgtt tagacggttc
     5401 tgaatctaac gtaattttaa caggtataac ttgagatact ttagtatagt taccatcact
     5461 atttgatgat ggcattaatg aaaagcttgc agcagtagct tttccaatac tatcaacttt
     5521 acctttaaca gaagcttttt gaccgtcaat agtcacatca acatctttac caacttcaac
     5581 atctttaata tctttttcgt caatatttgc agttacatat aaatcatcta aattgtaagc
     5641 ataagcaatt ggattgcctg cttgtaccat agaaccttcc atgccatcta atttagcaat
     5701 tgtacctttt tgtggcattt taagttccat atctttagtt tcgccatctt gaccttgaac
     5761 agtaactgtt gctactttat cacctttatc aagtttgtca ccttgcttaa cgtttagtga
     5821 tttgatttgc ccagaagctg gacttgctat tttaatttga tcgccattta ctttagcatt
     5881 atcagttgtg atataacttg ttgttttatt ccagaaataa aaaccagcaa tcccaattgc
     5941 tagcagtaca acaaccgtaa tgacattaat taatatcatc tttttcatga aacatttcct
     6001 cctatttgct tttaaaattc actttattta ttatagagac atgataacta aaaaaacacc
     6061 aacaattttt ataagagtgt tggttaaatt tcacaaaggt gtaacaatat gaaacgacaa
     6121 gccaaaatag aaattcaaaa cgcacttgtt gatttgatgg ctgaatatcc ttttcaagaa
     6181 atatccacta aaatgatttg cgcgtattgt aatattaatc gttctacttt ttatgattat
     6241 tacaaagata aatttgattt attggatacc atcaactcaa agcataaaga aaaattccaa
     6301 tttcttctaa gtgcgctaca tcataatttc gaaaatatta aacaagataa gcttaaactt
     6361 tataaattct tcattattat tgctaagtat atcaagcgca atgagcagtt tttcaaagac
     6421 attttagtta cctatccaat gaaaacatta ttcatagact atataaattt agcgagagat
     6481 tactatcaac aaattatgaa tgactatagt actacagtaa ccaataaaca gctttatgtt
     6541 acatatatca taggcggtca agctggggtg tttattaatt ggctttcgaa tggttgtaat
     6601 gaatcacctg aagaagttgc tgacatttta ttggctaata cgattaaact tcaaaaccaa
     6661 taatacggtt tcattctaat aaagaaatgc tataaaacaa ctagataaag ttaaaccaaa
     6721 tggacagata atagtacata tgtaacggtg ctatcaatat aaaaatgccg agatttccac
     6781 ataataatct cggcattttt atttttgatg ctaaattcat aggtctatga atagctcaag
     6841 ttttgttttt taatttttca attcaatata atagaagaaa ctatgtttat catcgatttg
     6901 ttttaaatac cttcatatta taaatctgta aaataattaa tataactgct gtagctataa
     6961 taataattat gtaaggtata ggattatctt caccttttac ccccactaat ggcgatgcaa
     7021 taccaccaaa taaaaactgc actaaaccta ataaacttga tgaactccct ctaccactac
     7081 tactttcatc catagctatt gtaaaaccaa gtgtcgcaac acctgtcact ggtgctatta
     7141 aaatgataaa tcctatcgct aaaatccaaa agttccaatg attaagcaat acaatcgtca
     7201 ctaaaattac accgataatc tgaatcatcg tcatgactct catcaatttt tgtgagtcta
     7261 taaaatcaac aaggtagcca gtaagttgac ttgatataat gagcgtaata ccaattcctg
     7321 caaacatcca actaaattgt attgcagtca tgccataaat tttttgaata ataaacggag
     7381 acgcagaaat ataagtaaat aatattacaa atgtcatacc ttgaatgagc attggtaata
     7441 caaaacgtgg cgtcttcaat aatattttga agtttttaaa cattgtcttt aatccactac
     7501 ttgattcacg atttgtcact gttaatgatt caggtacttt taataaagaa cctatgacca
     7561 taacgaagcc aaagatagtc aaaatgacaa agaccatacg ccagacagaa taatttaaaa
     7621 ttatgcctcc tattgttggc gcaacaactg gtgcaatacc attaacaagc atcaataatg
     7681 ccataaactt agttagttca ttaccactat acatatcact tgctatagct cttgaaataa
     7741 ctgctgctgc tccacctgtc actccttgaa gaaatcttaa tgcaatcatc agccaaatat
     7801 tatgtacaaa aacaatacct aaacttgcta atgtaaaaat aatcatagct ataataagcg
     7861 gttttctgcg tccatttgaa tctgaaattg gaccagcaaa caaattacca aacgctaaac
     7921 caatcataaa catcgataat gttaattgtg cgttagacgt cgttgtttga aaatcatgtc
     7981 taatatctgg taatccaggc aaaaacatat ctattgataa tgctccaatg gcagtcaaag
     8041 atcctaaaat aataataaat atcggtgaac gcttattaaa acgctttaaa tcattcacta
     8101 tatttcctcc tttatataat gattagttta taactatcac gaacagaaac taaatttaaa
     8161 tatgctcttt tacatattag cattactcac aagtttcata aacatcatta caacttataa
     8221 aaggtataaa atgatataaa cctcagcact ccataaattt tttcacaaaa acaatccctt
     8281 caaagtaatt cacactaatt gtaaatactt tgaaaggatg ttatttgtat tccatgacac
     8341 atgcatctta tttaagtttt acactctatt tttctgatgt cttgattaat tgcaagcggt
     8401 cttgcgaacc ttgttcaagc tcatattctt tatctacttg cttaccgttt tcatttatta
     8461 atcttacggt tgcggtaatc ttatcaccat gtttttcggc atctataatt tgtggtgaac
     8521 taatcatcat taaacttcct ttagatacac gcttctttac atcatcataa aaagatgatc
     8581 ctttttttat atatgacgat acaaaatcaa aatccgactg attaaacgca gcattattcg
     8641 ctaaagaata tccagcaaag aattctatca atttgttctt caagctgttt tcttcttttt
     8701 ctttcttttc aacatagtct tcgatatctt cactgtcaaa attcaaagtt atctctgtat
     8761 tgtattttaa atcgctagct ttaatcgtct tcgtctgtgt tgtaaatgtt ttatctttcg
     8821 ctttaccaga agctgaaatg gtaatatctt tattttgtgg atatggacca tacgttttga
     8881 aacttgaaaa tgccatttca cggtcattta tagttacttt tttagaacta tcatttaatt
     8941 ttgtatcgcc ttttaaagtt acagttatat ttgcttcttc aaaatcttca gtaacatcta
     9001 ccgtttcaga attactttgt ctaaaatcaa attttaaatg gcctgcaaaa tcaccgttct
     9061 cagttgattt catagctgga attctatatg tccccggtat aaaattacct attggcgtca
     9121 ctttatttgc ttcagctata accatttttt tcttaccacc agatttaaac tcatattttg
     9181 ttttttcttt cggtttaaca attggttgct tggtaggtgc agtaaagctc atattatcga
     9241 aaaagatata acgagtgcca tttttactta cacgtaatat attttgacca gatctggttt
     9301 gaatataaga agctacgctg gtcttactct tattcaattt atgtaccgta tttttaaggt
     9361 cgctgacaaa ttgcttaagc ccaacttcat ctttgatata gttaatgtat actttcgctt
     9421 cttcagaatc tactttatta tctttagtac ttaataatgt tgctactttt tgtttatcat
     9481 tattctcaat cgcattcact aatattttag tttgcgcttc aggtgaattg aagtttctta
     9541 gtaaaaagaa caatataata attaagatta aaatgaaaaa accaattgcc caaggcacca
     9601 tttttctcat cttgatgtta ttagtttgta tatcttcatc ggttgacttt ctatataatg
     9661 cttgacgact atcaaattta tgtccacatt gtgtacatat ctgtacatca tcttgtgctt
     9721 gttgtccgca cttcgggcaa gatttcatac tttcacctca attctgttcc tcttcaattg
     9781 ttatatgtat atcacaaaaa ttatttcatt attttaccat tattatttta caaatattat
     9841 cccaagaata caattcaaga aatgattaac tttactttag aattgtacct tattacatta
     9901 tttatgaaag tactctctca attataacta aaaagaaggt gttcaataca tacatattta
     9961 gtcttatatt tgaacacctt caaaaaccta tttatgattg ctatatgttt taatgcgatg
    10021 attgatgact tctaatactt gccttacatt ttcaatatct tcagaattta aatcattagt
    10081 aatatcttgc gcaatatctg tcataatcgc attacgttct ttcaaatatg ctctaccttt
    10141 gtcagttaag gttatgaatt tcaaacgttg atcaatattt aaatttggtt tatctaactt
    10201 aactaattta gcatcgatta actttttaat tcgtcggctt actgcggcct tgtttacacc
    10261 ttgtctttgc gtgatttcac taattgtcaa aggttcttta tttaacatac ctaatacgtt
    10321 agactgctct aatgaaattt catattcatt ttgtaaatct ttcaacattt ttgcagttaa
    10381 tgcgttaacg ttatttataa actgctctaa aaattgaata tggtcttgta aatgttttac
    10441 cataattatt tctccctaat tactattttt ataaagtatc ctcagcaatc attgtatcgc
    10501 acatctaaaa aacacaaaaa tattgaccca tatataatat aacaaattaa gacttacatg
    10561 ttgctacgtt ctccttgctt taataaatta aatcatctac atttatactt aatatttatt
    10621 ataagcgagg tgtcagtatg ttattgaatt tattattatt atttatactt atcgttattt
    10681 gtccgttaat tttcaactat actctgacgt taaatttaac atgctacatc ttagttgtca
    10741 tatcgatatt tttgtcactc atctatagca tcatgaccta tactttccct tggtctatgt
    10801 ttataacatt tataataatc agtattatca tgctattcaa acatagatat ttattttctc
    10861 atacttctag tcgcttattt attaaaaaat tattatcttt ttcatataaa tttgtactct
    10921 acacatcagc acttctattt ataagtacga ttcagtcacc tattgtgaaa ggtttgtcta
    10981 tgtggttagc tatattatgc ctttccttgt tactaacctt tatttgttat ctcgtttgga
    11041 cagtatcata tagacataga tcatacaaca aagaagttga tatcataatt gtgttaggtg
    11101 ctggtatttt taccgaattt gtcacaccaa tgcttgcagc gcgtttagac cgtgcacttg
    11161 atatctatca acaacaagta tcagcaacaa agattattgt atctggcgga caaggaccag
    11221 acgaaccaat tcctgaagct ttagctatgc aacgctattt gattgcacat ggtgttgata
    11281 aaacagatat actaatggaa tctcactcta taaatacata cacaaacttt ttatattcta
    11341 aacaaatcat caacaatttg tatcatgaag cagttaaaat tgtttgtgta acaagtcagt
    11401 ttcatgtatt acgcgcctta cgactagccc aaaaattagg tttatctatc gatggtgtcg
    11461 gaagtcatac accatatcac tttttcttac atgtgctcat taaagattat ctaggcgtaa
    11521 tgtatcagta tagattgtta ttaacgttat attgttcaag tagctggttt atatgtctat
    11581 acgctgcttt tatttataat agttaaccca caaataaact attattatag gttgtttcta
    11641 atattgcgtc aaactatctt taaatatgcg ttaatccttg attagacaat tgttatattg
    11701 aaaataaata aactaaagca cgtcatcatt ttatataata gtgacgtgct tttttgtaaa
    11761 cccaaatcgt attctgttat ctaactttta tactactatg tcttaaatca ttaacattta
    11821 gcgtctttac gagccactaa tcagttattt tgccatcttc taattcaatc actcgatcag
    11881 catattcaaa taatcttcga tcgtgtgtaa tcataatacc aatcatttgt tgttctttaa
    11941 tttgttgacg tatcatctca acaacttttg ttgctctatc ggcatctaaa cttgctgtgg
    12001 gctcatctgc taaaatgatt ttcggattat tcataaatgc tctcataatc gcaacacgtt
    12061 gcttttcacc gccagataac tgatgcggat atacattcaa gcgatcttct aaaccaatat
    12121 tttccaaaag ttgtattgct cttgttgaac tttgttgttt ggtcattccc gcttcttggc
    12181 ctacgagcgt caattgctct atcactttta aataaggaac taaatgtgaa gattgaaaaa
    12241 taaacccaat atcttccaat cgtaaatcac taggacgatg ctgtttatca aataatggcg
    12301 catcattgta aagcactgta ccactcgttt gacttaacaa tccacctaat atcgttagca
    12361 atgttgtttt cccagaacca gaggcaccat ttaaaatgac aaattcccct tgttccactt
    12421 caaaattaat accttttaaa acttttgttt cagacaaacc ttctccgaaa tttttgacga
    12481 tatctttaac aactaatgcc attattctgc acctccaatt gcttcgatag gatccacttt
    12541 aaataatttg acaaatgata gtgaggcacc taaaatcgct actaatataa atatccccac
    12601 catcaataaa atattttgcg ttgttaaata aaaaggcatc gttacaggca tcataaatga
    12661 tagtcctact atgatgatca cagcaatacc taccccaatt attgttagtg ttaaaatttg
    12721 taaaactaac gcactcaata aatgtcttgt cttaatacca attgctttca aaatgccgat
    12781 ttgtgatatt ttttgaatcg tcataacata gaaaaatgca cttagaacga ttgctgtaat
    12841 agcaaacaaa ctaacaatca tcatatttaa cggtgcttgc tctgcttgat aactcgcaat
    12901 attacctgtt aaatcttttt cactcacaac ttgaacgtca tttattttat taagctcatc
    12961 acgttgttgt tgcgttaaat ttttcactgg ataaaatgcc gtattaacct tattcaatgc
    13021 attaaatccg ttatcgttca atagcacaat ggaactatgc gcatacattg tgtcgtttaa
    13081 aataccagaa actctataac tagttgaatt atttttaaaa tgtaatctat ccccgactct
    13141 aatgttgtct gcagctaact tatcattaat agcgacctca tgagcatttt taggataatc
    13201 acctttttta agttgtatgc gttgttgttt aacgtcattc attgtaatca tatcttgttc
    13261 tttattagat aatttcaatg tttgaatatt cattttatat ggttcttgat gaattacctt
    13321 tttaatttga ttctgttgcg tatcactaag ttgcgatttc tcaatttgcg gctctttcat
    13381 tttttgaaca acatattcat cattatcaaa gtgttcaaat aacgaaatat tctccctacc
    13441 aagcccttga gctaaaccac taataaataa caccataata cttaataaaa gaatgattaa
    13501 tgtgattaaa atataacgaa atttgtaaaa cataatctct tttatcgcta atttcatatc
    13561 gattcacttc tccctatttc ttctttagcg tcagtataag gttgaaatat gaactcaata
    13621 tgaacaattg tttttttacg caaaaatatt ttacatgtat aataaatgaa aaagataagg
    13681 gagtttatag ctatggtgca atgtcttgtt gtcgatgacg atcctcgtat cctaaattat
    13741 atagctagcc atttacaaac agagcacatt gatgcataca cacaaccaag tggtgaagct
    13801 gcattaaaac ttttagaaaa acagcgtgtc gatattgcag tggtagatat tatgatggat
    13861 ggtatggacg gctttcaatt atgtaataca ttaaaaaatg attatgatat accagttatt
    13921 atgttaacag cgcgggatgc acttagtgat aaagagcgtg cgtttataag tggtactgac
    13981 gattatgtaa ccaaaccctt tgaggttaag gaacttattt ttagaattcg tgcggtatta
    14041 cgtcgatata atatcaattc aaattcagaa atgaccattg gcaacttaac gctaaaccaa
    14101 tcctatttgg aactccaagt atctaataaa acgatgacgt taccgaacaa ggaatttcaa
    14161 ttattattta tgcttgcagc acgtcctaag caaatcttta ctcgtgaaca gataatagaa
    14221 aaaatttggg gctatgatta tgaaggagat gaacgaacag ttgacgttca tattaagcga
    14281 ctacgccaaa gattaaaaaa attaaatgcc acacttacaa ttgaaacagt aagaggacaa
    14341 ggctataagg tggagaatca tgtttaaaac actctatgct agaattgcga tttattccat
    14401 tacggttatt ttatttagtg cattaataag ttttgtatta acaaatgttt actatcatta
    14461 taatttaaaa gcatctaatg acgcgaaaat catgaagacg cttaaagaag caagacaata
    14521 tgaacaatca gctaaaccaa cgcacattca acaatatttc aaacatttag ggcaaatgaa
    14581 ttaccaaata atgaccgttg atcaaaaagg tcataagaca ttttatgggg agccgtttag
    14641 agaagatact ttatcacaaa atgctattaa caacgtatta aataataaag attaccatgg
    14701 tattaaagat aaaccattcg cattattcgt aactggattt ttcgacaatg taacagataa
    14761 tactgttggt attaacttta agacaaaaga cggttcaata gcagtattca tgcgcccaga
    14821 tattggagaa acatttagcg agtttagaac attcttagcc gtcttgttaa tgttgttatt
    14881 atttatctcg atttcattag ttatcgcatc aacctattca atcattcgtc cagtaaaaaa
    14941 gttaaagcta gcgaccgaac gcttaattga tggtgatttt gaaacaccta tcaaacaaac
    15001 acgcaaagat gaaattggaa cattacagta tcactttaat aagatgagag agtcattgag
    15061 acaagttgat caaatgaggc agcattttgt acaaaatgta tctcatgaaa tcaaaacacc
    15121 attaacacat attcatcatt tattaagcga gttacaacaa acttctgata aaacattacg
    15181 acaacaatat ataaatgata tttataccat tacaacgcaa ctgagcggat taacaactga
    15241 attgttgctc ttatccgaat tagataatca ccaacattta ttattcgatg ataagattca
    15301 agtcgatcaa ctcattaaag atattattcg acatgaacaa ttttctgctg atgaaaagtc
    15361 gttaatcatt ttagctgacc tagaatcaat cgatttccta ggaaatcaac gtttgttaca
    15421 tcaagcatta agtaatttat taataaatgc tattaagtat acagatgttg gtggtgccat
    15481 tgacatcgct ttgcaacata gtcacaacaa catcattttc acaataagta atgatggttc
    15541 accaattagt ccacaagctg aagcacgttt atttgaacgt ttttataaag tgagcaaaca
    15601 tgataatagc aatggtttag gtcttgcaat tacaaaatcc atcattgaat tgcatcatgg
    15661 tacaatccaa ttcacacaaa gtaacgagta tgtaaccacc tttactatta cactgccaaa
    15721 taattcacat taatctctac ctcctgaaaa aacatttctg tttttattac aggaggtatc
    15781 ttttatatta attaacgctt tctagccact taacatttaa tttctaccat tcaacaaaat
    15841 ttaaatatat cgatggtcat ttatggttta ataattttga aggaggcatt aacctatgat
    15901 gaaactcaat ttatttatca atgcaactga gacggaatct tatattgata tacatgcacc
    15961 taaaatgaat gatcacgttc aaagtattat taatgcagtc aatgatttag acaaatcaca
    16021 tacattagtc ggatacatag acaaagaaat ccatatcatt aatgtatcag atgttataac
    16081 attccaagtt attaataaaa atgtaacagc cattacaagc aatcaaaaat tcaaactaaa
    16141 attacgactt tatgaactag aaaaacagtt accacaacat ttcattcgta tttccaaatc
    16201 agaaatcgtc aacaagtatt acattgaaaa attattatta gagccaaatg gacttatacg
    16261 tatgtatctt aaagatgcac actacaccta ttcttctaga cgttatttaa aatctataaa
    16321 ggagcgctta tcaatatgaa aaatttgaaa aactcattat ttattagctt aatcattggt
    16381 ttgtcccttt cactgttctt tagtatgtta tttgcagacg gcaaatatta tccactgaac
    16441 ccacaatcaa ctattggcat attgtattat aatcatttca cagaaactac cgtaatgttg
    16501 atttctatca ttttatggtt actcataggc gtcgtctttt tccttggaga ttttatcttt
    16561 aaatacacag attggggcat tactaaagca actatagtgc atttcataac aacgtatgtc
    16621 ggattcttac ctttagcaat acttgccggt tggtttccac taacaataaa ttccctaatc
    16681 atatttacaa ttatctttat tgtggtttac acattgattt ggattattca attctttaaa
    16741 aacaagaact acgtagacac tattaacgag caactgaaac aattaaaatg atttttatta
    16801 aaattataaa agctatacat tgcattaaag tagtctcttc tgcaatgtat agctttatgt
    16861 tgtatttatt ttaaatacgc atgaactgaa taatatgttt ttactatttt caaattaaat
    16921 tgtaactgca actgtccttt acaacatcaa cttatcatga taaaaaccta acgaaatgca
    16981 ttttcaaata ttttattcat aatttctgct acttctttag gactctcttt atatcctcgt
    17041 ttgacccaat cttgtaacac accaatgatt gcattactca taaaaataag taaaaaatac
    17101 ttttccttcg tcgttaacat ttttgtattt aatactggta caacattaat ttcgatacgt
    17161 tgttgtagtg atttttttat gacttgatgt aatttataag cactctcatc acctaataac
    17221 atcaatatga gctgtccttc acttagccaa taataaatca tccttttaaa aaatactgct
    17281 tgtgtcatag gcctctcttt aatgaacttc tttttagcaa cttttaacaa tgtattaaga
    17341 tagctgtata taaaatcatc tttagttttg taatgtgtat aaaaggttgt ccgacttatt
    17401 tgactctttt cacatatatc tttaacagtt atatcattat atgccttcat ttttaataga
    17461 tttaccattc catcatgtat gcgatttatc gttttcgtta cacgactatc cgtaaaagct
    17521 gtttgatcca tatgccccac cttttttctg aacactaaaa gcaaatatgt tcactatctg
    17581 ttaatatttt attcgcaatc ttaatatacg cttaagatga tatataaata aagtgaaatg
    17641 tatctcaatt taggaggggt tgtcatggac gcaactatag cgaagttgga taaagttgta
    17701 aaaacgtata acaagcatca cgtattaaac gaaattagta tgtcaatcaa taaaggtgaa
    17761 ctgctagggt taataggtcc tagtggttct ggtaaaacaa caacaataaa gtgtttacta
    17821 ggtatggaaa aagtagattc gggatctgca gaaattttcg ctcataagat gcctaatcgc
    17881 aaagctttaa atcgattagg ctatatgggg caaactgatg cattatacga aaacttaact
    17941 gcttatgaaa acctagagtt ttacggtcgt ttgtcaggac tgaaaggaaa tgtattaaaa
    18001 gataatattg aaaaaaatat cgttctagtg aatttagagc aagcgttaaa taaaaaagtt
    18061 gcacagtttt caggaggcat gaaacgccga ttatctttag caataacatt gttggccgaa
    18121 ccagaattaa tcatattaga tgaaccaact gtaggcattg accctaaatt gcgccaacaa
    18181 atatggcaac aattcaagca aatgaccaaa gacggaaaga gtgtcgtcat cacaacacat
    18241 gttatggatg aggcggaacg ttgcgataaa gttggactta ttgtcgacgg aagaatattt
    18301 gctatagata cgccaaccaa tttaaaacaa agatttaatg tagatacaat tgaagcggta
    18361 tttattaaag ctgaggaggt tcgtcaatca tgaaaatttt agctattgtt aaacgggtat
    18421 taaaagaaat gttacgtgat aaacgtactc ttgcgctgat gtttctagca ccaattttaa
    18481 tattgacgct tatgtatttt atttttaacg gtgatgaaaa cgatttgaaa atcggtatcg
    18541 atacatctgt ttcatcaaat ataacaaaac aattgccatc atccgacata aaaactcaaa
    18601 cgttccattc tcatgaaaat atcagatcta aaatcgagaa taatcatctt gatggttata
    18661 tttaccaaga aaatcaaaca ttacatgtca tttatacaaa tgaagaccct aacaaatcta
    18721 atatgatcaa acaaatgatt ggcaaagcta tacaacaaga taaaataagc agtataaagc
    18781 atctaattga aaaagcacct ccgaccatga aagcaccaaa caaacaagat attaagattt
    18841 cgcattccta tttatacggt aattctgata gtacttattt cgataaaatg tttccaatac
    18901 taatgggctt ctttgtattt ttatttgtat ttctaatttc aggtatagct ttattacgtg
    18961 aacgtacaac aggtacgtta gaacgtgtat tatcaacttc aattcgtcga agtgaaatcg
    19021 tccttggtta tctgattggc tatggtatat tcgcaatcat tcaaacatta atcatagtcc
    19081 tattttcaat atatttattg aacattaatt tagcaggtag cttatggtat gtaattttga
    19141 ttaatatttg tttagctatt acagcattat ctatggggat tttcatttca acgtttgcta
    19201 attctgagtt tcaaatgatt caatttattc cactagttgc tgtacctcag gtattctttt
    19261 caggaatttt cccacttgaa aatatgcctg attggctagg taacatcggc tatctcttcc
    19321 ctcttcgata tgctggtgat gctttaacta atattatgat aaaggggcaa ggttggagta
    19381 atatatggtt cgatttacta attctaatga tttttatcat agtctttatt atcttaaata
    19441 ttgttggttt aaaacgatac agacaggtat aaatgctaca aaacttttaa atgacttcaa
    19501 tcaagataag cttctaaata taaagcactg ataataaaaa caaccactaa aaaacggtaa
    19561 tgtctatcgc attaaaaaag acattaccgt ttcgtttatt atttaacttg taaatactta
    19621 gttacttctt cattaatttt gtgatataaa tcctcatgat ctgttaagcg ataaccaaat
    19681 gacggcacca tttctttaat ctttggttcc catcctttga attcatcacg atagcatcgc
    19741 tgtaaaacat caaacataat atctacagct gttgacgcac caggtgatgc accaagtaat
    19801 gcagctaatg tgccatcatc tgacgtaatg acttcggtac caaattgtaa gttaccttta
    19861 gaatcctcag tatccttgat tacttggaca cgttgccctg ctgtaatcac ttcccaatct
    19921 tcatttttag catttgggaa aaagactctt aaatcatcca tacgctcatc attagataac
    19981 atcaattgtg acactaaata cttcgtaaga ctcatttctt tgatacctgc agataacatc
    20041 gtcacgatat tatttggttt aaccgattta attaaatcca tatgtgaacc tgtttttaaa
    20101 aatttaggtg agaaacctgc aaatggacca aataacaatg aacgcttgcc gtctacaaaa
    20161 cgtgtatcta agtgcggcac tgacattggt ggcgcaccca ctgctgcttt tccgtacact
    20221 ttagcatgat gacgatcaat cacttcttga tttgtacagc gcaggaataa tccacttacc
    20281 gggaaaccac caatatgttt tgattgttta attccagtct tttggagtaa tggtaaactc
    20341 gcaccgccag caccaataaa tacaaaatca gattcatatg ttgtcgtttc gttagtttct
    20401 aaatctttaa ctttaactag ccatgcccct gatttctgtt tcttgatgtc taatacattc
    20461 tgcttatatt gaatttccac accacgttgt tctaaattat caaataactt tgcagttaac
    20521 gcaccaaagt taacatctgt accagtttca tcataagtta aagcaattgg ttcatcagac
    20581 ttacgacctt caatcattaa cggtacccaa gatttcattt cttcttcatc ttgcgataat
    20641 ttcatttttt cgaataaaac acttttcttt aacgttgcaa cacgactttt tataaaagct
    20701 acattatctt cgccaatgac aaaactcatg tgtggcaccg cttgaataaa gcgatctggg
    20761 ttatctaatt gtcctgtttt aactaaatat gcccaaaact gctttgaaat ttggtactgc
    20821 tcatttatct taattgcttt actacaatca actgtgccat ccttaccttc ttttgtatag
    20881 ttcaactcgc aaagtgctga atgccctgta ccggcattat tccatacatt tgaactctct
    20941 tcgccaggtt gtgctaattt ttcaaacact ttaatatttt tctcaggtga taattcttta
    21001 agtaatgttc ctaatgttgc actcataata ccgccaccaa ttaagatgac atctgttttg
    21061 ctatgttgtg ttgtcataac aaatacagtc ccccttttta aaatgaataa tggaacgttc
    21121 gacaaatacg acatattaat accactttga atcaataagt gtctcaatag gctcccatag
    21181 tgatgctcta ttactatcta ctatgtatat taatatttta taataacgaa actactttaa
    21241 ttatatactt tatgtgcgat agcacaaaga caaaaccaca aaccttaaca gctatcaacc
    21301 aatttaattt gattgctgat tactcactct tcattttaac ataaagctct accattatta
    21361 taatgtcaaa cttctttcat taataatcca ttaaaaaaag atgagatgtt agaaaacatc
    21421 tcaccctctg tatatttata ttttgaaata cgttttttta tagtaatgac aagatgaaag
    21481 tccaaataca gatgaatatt agtaaacata cactgtattt caatgtcatt tttaataatt
    21541 ctgattcctt accaacttgt tttactgctg cagttgcaat tgcaattgat tgtggtgaaa
    21601 tcaattttgc cgctacacca ccaactgtat tagcagatac aagaagtgaa ccacttgttc
    21661 caatttgttg tgcaacagaa gcttgaattg gtgcaaataa tgagttgtta tttacaactg
    21721 atcctgtcat aaacacacct atccaaccta agattggtga tagaactggg aagacattac
    21781 ctgctttagc aataccttga cccattgctg cacttaaacc accataagtt gtgattttag
    21841 aaattgctaa gatgaaacaa attgtaagga ctggtaacca taattcttta aatgtaacac
    21901 cgaataatct acctgcatct ttaaagttaa cctttttaga cattaaaatt gtaataataa
    21961 tagttaataa aatagctgta cctgtttgtc caataatatt taaagtcaaa actaatggtt
    22021 tatgtgtaac ttcactgact gttccaggta agttgtattt aaatactaat gatgataaag
    22081 caccatttgg taagaataag tttttaaaga atggcgcact ccaaatcatt acaatgactg
    22141 ttaatacaat gaatggactc catgcatgta atactgcttt tgcagaatgt gcttttgcag
    22201 gttcaatttc ttcatcttta ttaacacgat aaatgtgttt tggttggaat tttttagaaa
    22261 atactgctaa tgctaacatt gttaataacg gtggaataat atctgctaat tcaggtccac
    22321 tgaatactgt taataatcct tgagtaagtg tataagtgat tgaaactact aaaattgctg
    22381 gtaatgtttc tttaacacct ctaaaaccat caataataaa gataagtaag aatggaataa
    22441 tgaaatttat gattgccaat gttaaagttg ctgattgaga aacacctaat actgaaacat
    22501 ctccaggtaa tttcaacgtt tctacaacac ctacagggat accaatcgca ccaaaagcac
    22561 cagacgctgc atttgcgact aaacataaca tcgcagcttt taatggatta aatcctaatt
    22621 gtgttaataa aagtgcacaa attgcaattg gtacaccaaa tcctgctgca ccttctaaaa
    22681 atgcgttgaa tgcaaatcca ataagtaaaa cttgaatacg ttggtcttgt gaaatatttg
    22741 taatactatc ttgaattgtt aaaaattgtc cagattcaac agtaattttg tataacaata
    22801 ctgccatcat aacgatatag ccaatcggaa tgataccttg gaagaaacct tcgactactg
    22861 caccagaagc gataccaact ggcaatttga aaaatggtat tgcaattagt aatgttacaa
    22921 caagtgttgt aatggctgca taaatacctt tcattttaaa aacagttaaa catagtaaaa
    22981 acagtacaat cggtattgct gcaattaaag acgataataa taaattatca aatgggttaa
    23041 acgtatttac taacataggc tgactcttct ttccataaag tagtagtgta attacttcat
    23101 taaaatcatg ctctggctat agcattttgt tatcgataac attttacaga acagtgagat
    23161 taatgtgatt tttttcacaa ttaaaatata ccattttttc agactattaa caatatcact
    23221 ttcctattta attgttttat ttttcatttt aaatctgttc atcaaaatga attgtactta
    23281 atgtaatgaa gtaacttata ctattcatga attttacata agaccgtgct aaaataagga
    23341 agatactacg atgagaaatg gaagttatag caatgattaa acagataaat gtttctaata
    23401 tgcaaaagtt tgaatctcaa ttaatgaaag ctcagtctga aggttataca catgtcgttc
    23461 catatgccaa tgagataatg atttatcaat ctatgttaga tgcggttcaa ttatatccta
    23521 agtctatcgt ggttgattat actgtggatg gacaatacaa aaatgattgt cactatttcg
    23581 gtcaatcaac gatcaacatt gccgattggg ctcaaaacaa taattactat cctaacctga
    23641 tatatgcgat tcaacagact cttgatttaa tacattatta ctctgtagaa acgattttcg
    23701 atttagcttt attgacactt ctgaaaggtg atttatcaat cgatggtcat gtcgtttttg
    23761 attttaaagc ccctttagca acaagtgctt caatatggga aactattaaa actattgaag
    23821 attttgatat gatgtctcag ttttatttga ataaaatggc ttatattgat catcatccta
    23881 taccttttcg caaccttttt atcgaggatt cagaacaact taattcgcca gataattggt
    23941 tgtattctac caagtttatg ctacctaaat ggttatacaa aatagcaaag caacgtgctg
    24001 ataacaaaca attacaaaac ttcggcttgt atacgaagca accaaacgtt ttaaaagatc
    24061 acatcgtttt tatcggtgat catcatcaat atataggtaa ttcaaaatat ttatttactt
    24121 acttcgttaa acataatcca atgactgcct gttattttgt tactgacgat cgtcgcggac
    24181 cacatttcat atcacctaag tctgaaaaag ctgacgaatt gattaacagc gcacgcgtag
    24241 ttttagttga aaatgatata cctgaaacgt tacaaccaaa cggtacttta attcaattac
    24301 atcaaggcac accaataatg caattatttt tagatagtaa agagcctatt aaaaatatag
    24361 aaacaccttt ttatcgtgct aaaaggtata atcgctggtt gcaattcgat tatgttatac
    24421 attctgctga tgatattagc cacttttatc aaacggcttt tcctagtcac caagcaaatg
    24481 tattggctta cggtaatcct aagcaccaat atttactaca aaagcgtaac gaaagtacta
    24541 ctcaacaaca gtataagaaa tcgtttaaaa taaatgatca aaaaccagtt ttattatacg
    24601 cacctattgg attagtgagt gcgcaacaat taccactatc agatgcttta ttcaaagcct
    24661 atcatgttgt ggttcagggt attgatgaaa cgatgttacc agaagaggcg attgttgcac
    24721 caaaatatct tagtgctcaa gacttaattc taatgtctga tgtcgttatc actgattact
    24781 caaatataat atttgatgcg atggctatag acaaatcagt agcattatac actccaaatc
    24841 acagtcaata tattgagtct caaggtgtta acgaagatat ttggcgtcat ctatcaaaaa
    24901 tttggtatac agaccgacaa ttattaatta ataatttaat ttcccaggca ataccagtga
    24961 ttaaatatcc acaaatacaa cataaagaac aaccattaga atctatttca caacttatat
    25021 tgtctaagat gacatctaat caatagtgtt taaattcatc agtggctgtg aatgaggttt
    25081 aaaagtacta taaaacgtaa acttttatac tttaaaatac gcaaaaaaac ggtaaaccct
    25141 aattcatatt atagagttta ccgttttaat tttttaactt gcatcataat tacattaaca
    25201 ttattgttgg tagtttggat cagtaaccat tgcttgtcca gtataatcaa ccgttacaat
    25261 tgaatatttt ccatttgcat ttgggtcttt aaaactaaac acatacttat aattaccatt
    25321 atgttcttca atagagtagt cattatacac tttattatta ctaccaaatt tatttgcttc
    25381 attattagcc gcatttaaag ctgtttggaa atttggcaat tgctgtaaag cttgagtttt
    25441 atttccatta aacggataaa tttgacgtgc aaccggcgcg gcattttgtc cataatatgg
    25501 tgcaacgtaa cttgattttt gattattatt cgcttgatta ttacttgatt ggttattatt
    25561 tgtttggttt tggtcattgt ttgttgcatt tgaattagat tgttgctggt tatcgtttgc
    25621 actattatct ttattatctt tgtttacgtc attactatca tctttattgt ctttcttatc
    25681 tttagatgaa tcatttgttt ttttatcttg ttgttcagtt ttcgctttat catctttttc
    25741 tttattactg tctttttgtt ggtcactatc ttgaccacat gcagctaaaa ataatgataa
    25801 tgctagtaac cctgtaacta atcttttcat acatatctcc tcctataatt cgatattcat
    25861 tgaataagct tgaaatacat atctaccatg tgtatctttt catggcttgt taccaaagac
    25921 gtcaacatta atatgatctg aattatcaca atatccataa ctacaatcta aggttctttt
    25981 tattataccc taatttttgt tcattattat ttaatttttg tgaattttat gttttctata
    26041 aatttaatta ttttacttca acaattcatt acgcatttag catttcaagg catacacaat
    26101 atttattact atgatttcat tttatctgct gcaaaaacaa tcattataac tctttttcca
    26161 taattaaatc tgtatccgtt acatcacctg tttgaaaatg atgttcacca accactttaa
    26221 atccatgacg tttataaaat gcttgagcac gaggattatg ctcccaaact cctagccaaa
    26281 ttttatgttt attatgttct tgtgcaattt tttcggccaa ttctatcaat tgtgaacctc
    26341 ttccgccacc ttgaaagtct ttcaaaaaat atatgcgctg tacttctaaa taggtttccc
    26401 ccatttcttc agtttgagca ctattaatat tcatctttat ataaccaaca ttcgcaccat
    26461 cttcttgata aaaataatga aatgaatcta catggttaat ctcttgtgta aatttttcta
    26521 cagtataatt gtctttaaaa aattgatcaa aatctttgtc atcatagtaa gaaccaaacg
    26581 tgtcataaaa tgttctagtt gctaattcaa ctaattcacc agcatcttgt tttgaaattt
    26641 ctttgattat cccagccata taaatcctcc aataaacagt gatcgaatca aaatattact
    26701 tatgttattt ttcagccaaa actatttaaa aatacattaa cacaaatcaa ttacaaattg
    26761 tattgattgt gtgtaacatc aataaatgat acatttattc cagtaaaatg gccgtatttt
    26821 caaaagagaa aaagagagga tgtatcgttg tgatagaaac atttaaagcg tttgtaattg
    26881 ataaagatga gagcggtaaa gtgacaccaa ctttcaaaca attatcgcct actgatttac
    26941 ctaaaggaga tgtgctgatt aaagtacatt actctggtat aaattataaa gatgctttag
    27001 cgactcaaga tcataatgca gtcgtaaaat cgtatcctat gattccagga atagatttag
    27061 ctggaacaat tgttgaatcc gaagcaccag gatttgaaaa aggagaacaa gtaattgtaa
    27121 cgagttatga cctaggtgtt agccattatg gcggttttag tgaatatgcg cgtgtaaaat
    27181 cagaatggat tatcaagctt cctgatactt taacattaga agaatcaatg atatatggca
    27241 cagctggtta tactgccggt ttagcaattg aaagacttga aaaagttgga atgaatattg
    27301 aagatggtcc tgtactcgtt cgcggtgctt caggtggtgt cggtacttta gcagtactca
    27361 tgcttaatga acttggttat aaagttatcg caagtacagg taaacaagat gttagcgatc
    27421 aattacttga acttggtgcc aaagaagtta tcgatcgact tcctgttgaa gatgatcata
    27481 aaaagccact cgcatcatca acttggcaag cttgtgtaga ccctgttggt ggcgaaggta
    27541 ttaattatgt tacaaagcgt ttaaatcata gtgggtcaat tgcagttatt ggtatgactg
    27601 ccggtaatac ttatactaat tctgtattcc ctcacatttt aagaggtgta aacattttag
    27661 gaattgactc ggtatttact gctatgaaat taagacagcg cgtttggcgt cgtctcgcaa
    27721 aagatttaat gcctgaaaat ttacatgaga tcaagcaagt tattacattt gatgaacttc
    27781 cagaacaact taacaaagta attaaacatg aaaataaagg ccgcattgtt atcgatttcg
    27841 gtgtagataa atagtattca tgaaaaagac atcccgttat gcgagatgtc tttttatttg
    27901 ttgtggatta ttaaatttcc gatacttcat caattaacat tttactttag tgtattttgg
    27961 aaagccatgt attttcagca cctatcatac ctacacatta agcaaaataa atgttgattc
    28021 gtttacaatt catatttaat ttctctttct atatacatca aaggcatata ttgcccaata
    28081 aacgactgtg aatgcacaac tatacaaaat aactctctca atatttgtta aaaataaata
    28141 gatcattaaa gctgataaca caagtgcaac aaaataatat attatagctt gtcttaatgt
    28201 atcgattttc attgttctaa ctcccttagt tacaaagtcg aaaattctat atcatatcaa
    28261 cttgaacttt accataaaac acacattttt ttaatatcat tacttagaaa aacaaaaaca
    28321 tactaagtta agctgtgtag aaaactcagt atgtttatcg gtacgatttt tcatttttaa
    28381 aatttgatat acataccgcc tgaatctggt tcggtaggca taaatccaaa ttttgtatat
    28441 aatttatccg ctgggtagtc tgcgatcaga ctaacgtatg cactttcgac agatacattt
    28501 ttaatatact tcataatatg ttccataatt tgactgccgt aaccttgacc ttggtaactt
    28561 ttcaaaactg caatatcaac aatttgaaaa acagttcctc catcgccaat cactctaccc
    28621 ataccaatta accgatcttc atcatacaag gttactgtaa ataaggcatt aggtaatcct
    28681 ttttcagctg cttcgcgcgt ctttggactc atacctgcat taatccttaa tgcgcaataa
    28741 tcctcgcaag tcggaatatc atatgtcact ttaaccatta tttaccccac ttttcatcac
    28801 acaatatatc aaactagtat aaatgtttat ttacaatagt cttattcgct tctttaaaca
    28861 cttcatgatg acttgaaaca taaccctctg cattcgcatc tggttggata tatgttttag
    28921 caaggttcgc tgcatttgca ccatcactaa atgcacttgc aattagatgt gattttgcat
    28981 catgataaac aatatctcca cacgcataga taccaggtat actagttgtc gtattaccaa
    29041 atcctttaac acgacaatca tcatgcatat ctagctttga agatgtttca ctcaataatg
    29101 tattacaacg atcaaaacca tgactaataa tgacatcgtc aaatttaact gtatgcgtat
    29161 caccactttc aacatgttcc aaaacaactt cacttatatg cgtttcatca tcattgccga
    29221 ccaagtattt aatacgtgtt ttcgggcata gtttcacatt taaatctgtc accagtgttt
    29281 tcatcgcttc atgaccactt acatcttctt ttcgataaac aactgtcacg cttttagcaa
    29341 tcttggcaat atcatgcgcc caatctaatg ctgtatttcc tccacctgat attaatacat
    29401 ctttatcttt aaaacgtctg taactttgta caacataatg taaattagtt aattgatatc
    29461 tctctacacc tttaacatct aattgttttg gattaataat acccgcacca attgcaatga
    29521 taactgcttt cgatgtatat atttctcccg cttctgtttc aacttcgaaa tgacgttctg
    29581 cctttttcct aatatctacc acacgttcat tcaaatgaac ttccggttta aaatataatc
    29641 cttgcttaat tgtatctttt aaaatttcat gacaaggttt tggggcaatg ccgccaatat
    29701 cccaaataat tttttcaggg taaattctca tcttaccccc taattcagat tgaacatcta
    29761 ttaatcttac agacatatct cgcaatccag cataaaagct tgcatacaaa ccagacggac
    29821 cgccaccaat gattgtaacg tctttcatta tgtgcctcct atgactctct atattcattt
    29881 ctttcattaa cgcgctcaaa ttgataatta ttatcattta aagacattat actattaata
    29941 tttatattgt taaaataaat cgcatagtta gccatgaatt atcaataact atcccatcac
    30001 aatataaaaa cacacctttc aaagtacgaa acaacaatgt tcctttgaaa gatgtgtata
    30061 ttttttagta ctagttatat tattttttaa aagactcatc acgtggttct ttaagaattg
    30121 cttgtcttaa aaggaaaaat agcaacaata aacctgcaag catacctgtg tgcccaatac
    30181 ctgcaaagcc tgcaaatgct tctggagagt atgatttacc agtgacttgg aagaatcctt
    30241 ttgtcaccat catacctata gtaacgataa cacctatgtt atatacatag aagaaccagt
    30301 taaataagta gtaacttgat aatttaaata acttctccaa tggtaataat attaaaaaca
    30361 taaacatacc taaaataagt gtatgtgtgt gtacaagtga taattgtgtt gtaccagtaa
    30421 aatcatatgc ttttgttagc tctctataga aaaatccact aaataaacct atgatcatat
    30481 aaaaaagaaa tgaatataat aacctacgca ttttgaatcc tccctcacct tttcattata
    30541 tccgattata cctattataa cagtgacaaa aaagcgaaga tatgacacaa ctgaatttgg
    30601 tttagcacat agtatgacag ctaaaataac tttacatcaa gtactataca aataaaatag
    30661 ctataccatg acgctcgcta caaacgtctt gtatagctat attcctattt attaaattat
    30721 gacggcactt cagtacgttt tgtgctatgt tttctaacaa tagctgaaac taaacttgat
    30781 acagcgccaa aaatcataaa ccctataaac atccacattg tactatttaa ttcaatttga
    30841 tgattcaagt ataatagttc tcttacactt gttgcatagt gtgtgaatgg attccaatca
    30901 tatacatatt tttgataact ttcgggcaac atttgtttag gtaacgttac aagttgcata
    30961 ctaaagaaca ttaaaatgaa gaaaattgga attgacttca tacctagcca taccataaca
    31021 cctaaaataa gacccacgaa accaagaatc gcaagtgcta caaaaattgc gatacgattt
    31081 ggatggtcaa aatcaaatcc ttgaacgcct tgcatgaaat aaatataaac gaagctacct
    31141 gcaaatgctg caacaactgc aaatatcatt tgtccaattg aagcaatgat acgatgttgc
    31201 acgacgatat tgttactagt tctaaatgca aagaacaata agatagacgt tacgattgaa
    31261 cctatccaaa ttggcataaa cattaggaat ggtgcattac cacctgcttg gtgatcttta
    31321 actttattaa gtttttcatt atccactttt actggattcg tcaaaccatt gatgtccgcg
    31381 gcattgactt taacattttg actcgttaat gtttccaaac tttgcttcgt aatttgagca
    31441 ttaatattat cacccatacc agttaacaca gctgatgcaa tttgtgaacc ttgtaagctt
    31501 gatccttcac ttacaatcgt tttaaattta gcctgcttaa cctctacttg ttgattcccc
    31561 attttttgtt tcatttgttg aaccacttgt ggcggaattt cacctgaagc aactttttgt
    31621 tgcatttctt cttttttact atccataact actttttgtg ttttactcat agcatctttt
    31681 gaaaagtctt tatcaataat agctactcca aagatttttt gatctttcaa atctttttca
    31741 aggtcttttt cactatcaac cttaacccat ttaattttat tagaatcact atctaataat
    31801 ttatcctcta attttttacc aatgttaaca tttttatctt gaatcgttgt acctttgtcc
    31861 tcgtttaata taccaattgg taaatctttc ggtttaggat tatatgcagg ataaaaagct
    31921 aaagaaaaga ttaccaagat aatcatagtt gctattggtg caatccataa taatttattt
    31981 tgaaatatat tcattttatt aattacctcc ttattagaca atgtgttgaa tatttcgcat
    32041 gttgtctatt ataatatgta agtgggaata aacaatacac aatgttcatc aatacgttta
    32101 ttaatagaca aatacaccca cattgttcaa taacacaagg aggttgattt atttttgaaa
    32161 gaagataggc gaattagaaa gacaaagtcc tccattaaac aagcgttcac taaattacta
    32221 caagaaaaag atttagaaaa gattaccatt cgcgacataa caacacgcgc tgatatcaat
    32281 agagggacat tttacttaca ttacgaggat aaatatatgt tactcgcaga tatggaagat
    32341 gagtatattt cagaactaac gacatatact caatttgatt tgttacgcgg ttcttcaatt
    32401 gaagacattg cgaatacttt tgtaaatgat atactcaaaa atattttcca gcatattcat
    32461 gataatttag agttttatca tactatttta caattggaac gcacgagtca attagaattg
    32521 aaaatcaacg aacatattaa aaataatatg caacgttata ttagtatcaa tcattctatc
    32581 ggaggcattc cagagatgta tttttacagt tacgtttccg gagcaacaat ttcaattatt
    32641 aaatactggg taatggacaa acagcccatt tcagttgatg aattagctaa acatgtgcat
    32701 aatattgttt ttaatggtcc attaagaata atggcagaaa accgtttgca taaatcaaac
    32761 cttgattcac taacttaagt aatactcgct tttcctaaat cctttagaaa ggaactatat
    32821 atcatgatta cttcattcag acattctgaa gatatagata aacatattat aaaaacacca
    32881 ttagatcata cagcgtcatg gattaatgta gtagaaccag accgagaaga aattgaaaat
    32941 cttatggaac aatataatat acctgaagac tttatacgtg accctttgga ctcagaagaa
    33001 agttcccgta ttgaatatga cgaagatact ggttactcat taatcattat tgatttacct
    33061 atcgtcaatt caactaatcg tagcgttcta tcttttgtaa cgattccatt aggcattatt
    33121 attggcaacg gtattatcgt aacagtttgt gacgctgaaa atgaattttt agaaaattta
    33181 cctaagcgtg atattaattt aaaatttcac agcagatttg cattagaaat tttaactact
    33241 attgccgacc attataatcg taacttacga ttacttaata aaagtagaat tcgtattgag
    33301 aaagaactaa aaaataacat tactaacaaa caacttttca aattaatgga agttgaaaaa
    33361 agtttagtat actttttagc cgccttaaaa ggtaacgata caattattaa aaagttattc
    33421 cgtttacctg caattaaacg ttttgaagaa gatgaagaat tactagaaga tttaatcatt
    33481 gaaaataacc aagccatcga aacaacagaa ttacatcaac gtatcttaga aagtatcaca
    33541 acatcatacg cttccttatt atctaatgat atgaatacga ttatgaagac attaacactt
    33601 ttcacggtac tattaacgtt accaacgctc gtatttagtt tcttcggtat gaatgtgccg
    33661 ttgccaattg atgaccatag ttatatatct tggattattg tggtaggtat ttcattaatt
    33721 cttgtcgtaa ttgttagtat tttcttatgg cgaaaacaga aattataact aaattaaaag
    33781 gtatgacagc ttaaagtttc accctattgc atttgcaatg actgatactt tgattactgt
    33841 catacctctt tttttattct acaacttctt tatttgcttt tcttttagat aaaattaaag
    33901 taactgttat ggcaacgatg aatgatattg tcataccaac aaagtagtgt aaccatcctg
    33961 catgtactgg attgattgag ataaatccag gcaatccagc agttcctaat gcgattgctt
    34021 taaccttgaa gaaagcaata tatgctgaac caatacctga tccaacgata gcgccaataa
    34081 aaggatatct tagttttaag ttaacaccaa acatagccgg ttctgtaata ccaagtaatg
    34141 ctgaaatacc tgcggcagat gccacacctt ttaacttctt attttgttta ataataaaga
    34201 acgctgcaat tgctgcacca ccttgtgcaa cattagacat tgtcgcaatc gggaatataa
    34261 atgatccacc cgtttttgtg gcatctgcaa ttaatgtcgt ttctacagct ataaagctat
    34321 gatgcatacc tgtaataaca atcggagcat acaataatcc gaatattaat ccaccaattg
    34381 caccaccaaa ttcataaagc catgttaaac catctgataa ccagtaacct aattgtcgag
    34441 tgattggacc tacaaatgaa aatgttagaa atgctgttat aaaaatagat aacaatggtg
    34501 ttaacaaatt atctaacacc gttggaataa ctttgcgtaa ccctttttca attgaagcta
    34561 agatataagc tgctacaagc ataggtaaca cttgtccttg ataacctact tgattaatat
    34621 gcaaaccaaa tacatcccaa tatggaatag ccttacctgc ttcaactgct tttgggaaat
    34681 cgtatgcgct cattagcgat ggatgaacca atatcatacc taatgcagca cctaaaaacg
    34741 gattgccacc aaaacgtttt gctgcactaa atccaattaa aattggtaat aatgtaaatg
    34801 gtgcattcgc aaaaacattt atcatttcag ctaatccagc aaattgacta tagacatcta
    34861 tcaatgattt acctgaaaag aataaatctt tcgcagtcaa aatgttattt aaccccatta
    34921 ataaaccacc agcaacgatg gctggtataa tcggaacaaa gatatctgaa agcattttta
    34981 caaatcgctg taacggattc atatttttag cagattgtgc tttgacttcc gaagtggttg
    35041 atgcttcttt tccagttaat ttttccagtt cactaaatac tttattgact gtaccagacc
    35101 caataataat ttggtattgt cccccagtag aaaacgtccc tttaactaca tccatattgt
    35161 ttagcgcctc ttcatttact aaactttcat catttaaaac taatcgtagt ctcgttgcac
    35221 aatgcgccat tgcatctaaa ttctcttctc cgcctatcgc gttcaaaata tcttctgcgg
    35281 attgcttata attcatatat ttagcctcct aattagaacc ggttccattt attccacttt
    35341 attgtaaccg gttccaaaaa tatttgcaac actctattat cattttatat aaacacctac
    35401 accatttttg atagcgcatt catttttatt gctatactat atgcatgtat attaacatta
    35461 attatcggag gtaattttat gaaagaacgt ggccatatcg aacagctttg gcgtgaggaa
    35521 aaatatcatg tattgttaca cagtcaacag tcttaccaaa tgataaggaa tgctttaaaa
    35581 acggatttat cacttcatca agtccaacaa atgattgatg atgctttgtt aattgaacca
    35641 agtataggaa gtgtatgtaa tgcttttgat catatgtggg gatattttaa aaaatgtgcg
    35701 aatgaagaag aaagacaaca atcaaaacta cttaaagctg attttattaa tggtaaaatc
    35761 gacacccaaa ctttattaga ttttttagca gaacttgcta ataaatatga tgttcaatat
    35821 ttactacaaa gtcgtgtttt aaacacaaaa agaaagagat agattgaaac tttagttgta
    35881 acggacaaat agtagtcatt ggagttcagc aattcatact ttgtactaaa gtttactata
    35941 atctatctct caaggtttaa atgtacatat ctatattaaa atgcgtttca tgatgttaga
    36001 agataccata cacatttaat tagttacagc ataaatcaat ttataccctt aattattaca
    36061 ttgtttatat tttaaattcg ggggagtaat tttaagtaat atcttgttgc tgctaattct
    36121 attgttaata aattgatatt agatgtagaa attgagtgtg aaagttaata atagatgatt
    36181 tagctagtaa cataattaat tcatatatag tcaaaaagtt ctgttagata ctgttcttga
    36241 tgaatattct ttgatgttag gtgttagcta ttctagatga gtttcttaga tgtggatgcc
    36301 tgtcttgatg aagttatcta tgatgttaat ataatacttg atgttgattc ttagatgtta
    36361 gtgcgatttc aagatgctga ttctttgttg ttgcactatc ttggtgacgt ttctagttgt
    36421 ttatagttat cgcatctctt gatgtaaaat ctaagatgtt tatgcatttc tagatgttaa
    36481 ttcttcgctg cttaagaatt atcttgatgg taaatctagt tgtgccatgc attttctcat
    36541 gatgaatcta aggtgttaat tcattgatgt gtgctgtttc ttgttgcttc attatcttga
    36601 tggtgaattt cgttgtctaa tgcacttttt caaatgatta ttctaattgt tacggcaata
    36661 actagatgga ttttcttaga tgttcacttt tctagatgtt tatacttatc ttgatgtttt
    36721 ctaaacagaa cttttaaacg ctttattttt cagtagattg tttaaaattt cttagttgat
    36781 ggttattgtt gttgatttga ataatttgta gcgaatattt tgtaacaggg ctactaagaa
    36841 tttttataac aagaagtgat ttcaatcgta ctaattttca gcgtttgatg acaaaagtgt
    36901 atgtccaaac ttaaataatg gcgataaaat ttaagtcatg ggtacattta atgttttttg
    36961 attcaaactt ccattttggt caatttgctt tttttataac atagttgaat atcaataaaa
    37021 gattagcaag taagttcaaa atggctatgt attgtttttt gaaatacata ggtttgaaat
    37081 ctatattaat tatgcaccaa ataatttaat tagactctat cgaaaatttc caaattttgc
    37141 ttactatctt tctaatttta cttttatatt gtgcacaatg cataaaaaca agcgttttcc
    37201 taggagtttt cattaatttc ttttattttt cactattacc ttttcaaaaa tattcaacta
    37261 taataggtat actatccatg attttaaaaa tttccatgtt ttacatttaa atgatagatt
    37321 tcaatatata ttaggcagga gagagaaata tgacatgcca acttaaaata catcata
//
LOCUS       DAHHOJ010000014        37374 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.014, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000014 DAHHOJ010000000
VERSION     DAHHOJ010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 37374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37374
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.014"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(162..2228)
                     /locus_tag="NBH32_000610"
     CDS             complement(162..2228)
                     /locus_tag="NBH32_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIABC"
                     /protein_id="HCD1514240.1"
                     /translation="MFKKLFGQLQRIGKALMLPVAILPAAGILLAFGNAMHNEQLVEI
                     APWLKNDIIVMISSVMEAAGQVVFDNLPLLFAVGTALGLAGGDGVAALAALVGYLIMN
                     ATMGKVLHITIDDIFSYAKGAKELSQAAKEPAHALVLGIPTLQTGVFGGIIMGALAAW
                     CYNKFYNITLPPFLGFFAGKRFVPIVTSVVAIATGVVLSFAWPPIQDGLNSLSNFLLN
                     KNLTLTTFIFGIIERSLIPFGLHHIFYSPFWFEFGSYTNHAGELVRGDQRIWMAQLKD
                     GVPFTAGAFTTGKYPFMMFGLPAAAFAIYKNARPERKKVVGGLMLSAGLTAFLTGITE
                     PLEFSFLFVAPVLYGIHVLLAGTSFLVMHLLGVKIGMTFSGGFIDYILYGLLNWDRSH
                     ALLVIPVGIVYAIVYYFLFDFAIRKFKLKTPGREDEETEIRNSSVAKLPFDVLDAMGG
                     KENIKHLDACITRLRVEVVDKSKVDVAGIKALGASGVLEVGNNMQAIFGPKSDQIKHD
                     MAKIMSGEITKPSETTVTEEMSDEPVHVEALGTTDIYAPGVGQIIPLSEVPDQVFAGK
                     MMGDGVGFIPEKGEIVAPFDGTVKTIFPTKHAIGLESESGVEVLIHIGIDTVKLNGEG
                     FESLINVDEKVTQGQPLMKVNLAYLKSHAPSIVTPMIITNLENKELVIEDVQDADPGK
                     LIMTVK"
     gene            complement(2529..4268)
                     /locus_tag="NBH32_000611"
     CDS             complement(2529..4268)
                     /locus_tag="NBH32_000611"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HCD1514241.1"
                     /translation="MAKIKANEALVKALQAWDIDHLYGIPGDSIDAVVDSLRTVRDQF
                     KFYHVRHEEVASLAAAGYTKLTGKIGVALSIGGPGLIHLLNGMYDAKMDNVPQLILSG
                     QTNSTALGTKAFQETNLQKLCEDVAIYNHQIEKGDNVFEIVNEAIRTAYEQKGVAVVI
                     CPNDLLTEKIKDTTNKPVDTSRPTVVSPKYKDIKKAVKLINKSKKPVMLIGVGAKHAK
                     DELRAFVEAAKIPVVHSLPAKTILPDDHPYSIGNLGKIGTKTSYQTMQDADLLIMVGT
                     NYPYVDYLPKKNIKAIQIDTNPKNIGHRFNINVGIVGDSKIALHQLTENIKHVAERPF
                     LNKTLERKAVWDKWMEQDKNNNSKPLRPERLMASINKFIKDDAVISADVGTATVWSTR
                     YLNLGVNNKFIISSWLGTMGCGLPGAMASKIAYPNRQAIAIAGDGAFQMVMQDFATAV
                     QYDLPLTVFVLNNKQLAFIKYEQQAAGELEYAVDFSDMDHAKFAEAAGGKGYTIKSAS
                     EVDAIVEEALAQDVPTIVDVYVDPNAAPLPGKIVNEEALGYGKWAFRSITEDKHLDLD
                     QIPPISVAAKRFL"
     gene            complement(4315..5004)
                     /locus_tag="NBH32_000612"
     CDS             complement(4315..5004)
                     /locus_tag="NBH32_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HCD1514242.1"
                     /translation="MNDYVQALLMILLTVVLYYFAKRLQQKYPNPFLNPALIASLGII
                     FVLLIFGISYNGYMKGGSWINHILNATVVCLAYPLYKNREKIKDNVSIIFASVLTGVM
                     LNFMLVFLTLKAFGYSKDVIVTLLPRSITAAVGIEVSHELGGTDTMTVLFIITTGLIG
                     SILGSMLLRFGRFESSIAKGLTYGNASHAFGTAKALEMDIESGAFSSIGMILTAVISS
                     VLIPVLILLFY"
     gene            complement(4997..5392)
                     /gene="cidA"
                     /locus_tag="NBH32_000613"
     CDS             complement(4997..5392)
                     /gene="cidA"
                     /locus_tag="NBH32_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holin-like murein hydrolase modulator CidA"
                     /protein_id="HCD1514243.1"
                     /translation="MHKVQLIIKLLLQLGIIIVITYIGTEIQKIFHLPLAGSIVGLFL
                     FYLLLQFKIVPLTWVEDGANFLLKTMVFFFIPSVVGIMDVASEITLNYILFFAVIIIG
                     TCIVALSSGYIAEKMSVKHKHRKGVDAYE"
     gene            complement(5605..6483)
                     /locus_tag="NBH32_000614"
     CDS             complement(5605..6483)
                     /locus_tag="NBH32_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000351767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1514244.1"
                     /translation="MDIKHMKYFIEVVKQGGMTNASKSLYIAQPTISKAIKDIENEMG
                     TPLFDRSKRHLILTDAGQIFYEKSKEIVALYDYLPSEMERLNGLETGHINMGMSAVMN
                     MKILINILGAFHQQYPNVTYNLIENGGKTIEQQIINDEVDIGVTTLPVDHHIFDYTTL
                     DKEDLRLIVSREHRLAKYETVKLEDLAGEDFILFNKDFYLNDKIIENAKNVGFVPNTV
                     AQISQWHVIEDLVTNELGISILPTSISEQLNGDVKLLRIEDAHVHWELGVVWKKDKQL
                     SHATTKWIEFLKDRLG"
     gene            6650..6868
                     /locus_tag="NBH32_000615"
     CDS             6650..6868
                     /locus_tag="NBH32_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1514245.1"
                     /translation="MSFYEFMQSFLGDDTPLGELVDWINQDSNFPREVKSHSEILSYF
                     RTNPCPESISVPVIKRALSVFNQFTNVQ"
     gene            7257..7688
                     /locus_tag="NBH32_000616"
     CDS             7257..7688
                     /locus_tag="NBH32_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1514246.1"
                     /translation="MEYKKILIRLLIAFAVLFSADFTYQSVEQTHQSHAAVNYYSKNQ
                     CTWWAFKRRAQVGKPVSNKWGNAKNWYNNARKSKYATGRTPRKFAVMQSTAGYYGHVA
                     VVEQVYKNGSIKVSEYNFYRPLKYNTRVLSKKAARNFNYIY"
     gene            complement(7827..9104)
                     /locus_tag="NBH32_000617"
     CDS             complement(7827..9104)
                     /locus_tag="NBH32_000617"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HCD1514247.1"
                     /translation="MQNLDKNFRHLSRKEKLQQLVDKQWLSEEQFDILLNHPLIDEEV
                     ANSLIENVIAQGALPVGLLPNIIVDDKAYVVPMMVEEPSVVAAASYGAKLVNQTGGFK
                     TVSSERIMIGQIVFDGVDDTEKLSADIKALEKQIHKIADEAYPSIKARGGGYQRIAID
                     TFPEQQLLSLKVFVDTKDAMGANMLNTILEAITAFLKNEFPQSDILMSILSNHATASV
                     VKVQGEIDVKDLARGERTGEEVAKRMERASVLAQVDIHRAATHNKGVMNGIHAVVLAT
                     GNDTRGAEASAHAYASRDGQYRGIATWRYDQDRQRLIGTIEVPMTLAIVGGGTKVLPI
                     AKASLELLNVESAQELGHVVAAVGLAQNFAACRALVSEGIQQGHMSLQYKSLAIVVGA
                     KGDEIAKVAEALKQEPRANTQAAERILQEIRQQ"
     gene            9358..10524
                     /locus_tag="NBH32_000618"
     CDS             9358..10524
                     /locus_tag="NBH32_000618"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HCD1514248.1"
                     /translation="MTIGIDKINFYVPKYYVDMAKLAEARQVDPNKFLIGIGQTEMAV
                     SPVNQDIVSMGANAAKDIITDEDKKKIGMVIVATESAVDAAKAAAVQIHNLLGIQPFA
                     RCFEMKEACYAATPAIQLAKDYLATRPNEKVLVIATDTARYGLNSGGEPTQGAGAVAM
                     VIAHNPSILALNQDAVAYTEDVYDFWRPTGHKYPLVDGALSKDAYIRSFQQSWNEYAK
                     RQGKSLADFASLCFHVPFTKMGKKALESIIDNADETTQERLRSGYEDAVDYNRYVGNI
                     YTGSLYLSLISLLENRDLQAGETIGLFSYGSGSVGEFYSATLVEGYKDHLDQAAHKAL
                     LNNRTEVSVDAYETFFKRFDDVDFDEEQDAVHEDRHIFYLSNIENNVREYHRPE"
     gene            complement(10695..11216)
                     /locus_tag="NBH32_000619"
     CDS             complement(10695..11216)
                     /locus_tag="NBH32_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCD1514249.1"
                     /translation="MVYKSYYDSPVGRLELLSDGVSLTAVLFENQQGDGNREENTSLA
                     IFKEATQWLDAYFKGDNPEITIPLKPTGSDFQQCVWNELRQIPYGTLTTYGAIAKKVG
                     KVLDKPKMSAQAVGGAVGSNPLSIIVPCHRVVGKTGSLTGFGGTINNKIKLLELENID
                     MSKLYVPKHSTKP"
     gene            11579..13696
                     /gene="clpL"
                     /locus_tag="NBH32_000620"
     CDS             11579..13696
                     /gene="clpL"
                     /locus_tag="NBH32_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001058986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpL"
                     /protein_id="HCD1514250.1"
                     /translation="MNNNFFNSDFDSILRRMMQDMQSSNQTSNKKYYINGKEVSPEEL
                     AQFTQQGGNHSAEQSAQAFQQAALRQQGQQGQQGGNGNYLEQIGRNLTQEARDGLLDP
                     VIGRDKEIQETAEVLSRRTKNNPILVGEAGVGKTAIVEGLAQAIVEGNVPAAIKDKEI
                     ISIDISSLEAGTQYRGAFEENIQKLVEGVKSSQNAVLFFDEIHQIIGSGATGSDSGSK
                     GLSDILKPALSRGEISIIGATTQDEYRNNILKDAALTRRFNEVLVNEPSAKDTVEILK
                     GIREKFEEHHQVKLPDDVLKACVDLSIQYIPQRLLPDKAIDVLDITAAHLSAQSPAID
                     KVATEKRISELEHDKRKAVSAEEYKKADDIQNEIKSLQDKLENSNGEHTAVATVHDIS
                     DTIQRLTGIPVSQMDDNDIERLKNISNRLRSKIIGQDQAVEMVSRAIRRNRAGFDDGN
                     RPIGSFLFVGPTGVGKTELAKQLAIDLFGNKDALIRLDMSEYSDTTAVSKMIGTTAGY
                     VGYDDNSNTLTEKVRRNPYSVILFDEIEKANPQILTLLLQVMDDGNLTDGQGNVINFK
                     NTIIICTSNAGFGNGTDQEYDDIMHEMKKFFRPEFLNRFNGIVEFLHLDKDALQDIVN
                     LLLDDVQVTLDKKGITMNVSQDAKDWLIEEGYDEDLGARPLRRIVEQQVRDKITDYYL
                     DHTDVKHVDLDIEDNELVVKGKS"
     gene            complement(13763..13933)
                     /locus_tag="NBH32_000621"
     CDS             complement(13763..13933)
                     /locus_tag="NBH32_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HCD1514251.1"
                     /translation="MSVIINILIFLAIFGYALYTLVKFFKRSKQGKCGTCDIDRDCCG
                     TEQHTANHFPGK"
     gene            complement(13946..15940)
                     /gene="feoB"
                     /locus_tag="NBH32_000622"
     CDS             complement(13946..15940)
                     /gene="feoB"
                     /locus_tag="NBH32_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HCD1514252.1"
                     /translation="MENYCILGNPNVGKTSLFNALTGSYEYIGNWSGVTVEKKVGKLK
                     ENVGQLIDLPGTYDLSPISKDETVVTDYLLNDSFSGIINIVDASQLKRNMQLTVQLLE
                     LNQPIYIGLNMIDVATKRGIKIDYHKLMKKLKTPIFPVVARTGKGTKHLLGEIKHLGE
                     GYQPHFKINYGEKIEETIKNMCQIIMTETSHDKYQARFIAIQFLLNNMQIANELNSEV
                     VNKLSSLRDQVAKQVEAVSVRREMERIRNHYIETLLQDVVTYPDEDKQYFSSRIDKIL
                     THKYIGMPIFLAIMWLIFQTTFTWIGTPLSDQLDAFIGGTFTDSVKTIMNYLGVIPFL
                     QDLITDGIIAGVGSVLVFVPQIVVLFFFISLLEDSGYMARIAVLMDRIMESFGLSGKS
                     FIPMIIGFGCNVPSIMAARSIENEKERLTTILIAPFMSCSARLPVYALFVGIFFKENQ
                     SLVVLSLYVLGIIMAFLVSTVLTKTVLKNDNAIFIVELPTYRVPSIKTLWRSTWEKAK
                     GFVRKAGTFIFGGSVVIWLLSYVGPHGINVNINQSFLHMVGSFFGMLVQPLGFGTWQA
                     GATLVPGFLAKEVIVSSMAIIYSSGDAGLVNVIQNQFTPLSAYAFMIFILLYIPCVST
                     VAAIRKETYSWKWTALAVAYPLVTAYVLTFIFYQVGHLFV"
     gene            complement(15952..16179)
                     /locus_tag="NBH32_000623"
     CDS             complement(15952..16179)
                     /locus_tag="NBH32_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HCD1514253.1"
                     /translation="MLNIKNGEMNKAYKIKRMDIANENMLYRLSAFGLTDDAIITIKQ
                     KCLFKGPCIIEVNGQQLSIRHCDACSIALEE"
     gene            complement(16395..18884)
                     /gene="farE"
                     /locus_tag="NBH32_000624"
     CDS             complement(16395..18884)
                     /gene="farE"
                     /locus_tag="NBH32_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001051413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux MMPL transporter FarE"
                     /protein_id="HCD1514254.1"
                     /translation="MAKFLYKMGTFIAKHKWSAVIAWIVIVAAILIPLATNAPKFDND
                     IKMTGLESLDTNKKIEKHFNQDSEKAQIRVVFKTTKDDGIVQPNITKDIKKTLDDIKK
                     DDKHIDKISDPYENKQISKDKTTAFADITYDVSQTSLKDGSRDNVKSHLKDLRDNHNV
                     QTELTGTGMTSTEVGGNSELVGIIVAFVVLLITFGSVIAAGLPIISALIGLASGVGII
                     SLLTYAFDIPNVTLTLAVMIGLAVGIDYALFILFRYRQVMKTETDYVKGIGLAVGTAG
                     SAVIFAGVTVVIAVCGLSLVGIDFLAVMGFASAISVVFAVLSALTLLPALISIFHKRI
                     KVNKLQSKFKKDIDTPWSKFITGNALAAVLLGLIILVAAAIPVSHMRLGIPDDGVKPA
                     DSTQKKAYDIISDKFGEGFNGQIPMLINVKDKKDDPQGLQQDLQSVYKDIKDKKNVDI
                     VTPPQMSKDNDYALMVVIPKQGPNAESTNDLVHDLRDYHRDAQDKYGFKTEISGQSVI
                     NIDMSKKLNEAIPLFATVIVVLAFFLLMIVFRSILIPLKAVLGFVLSLMATLGFTTLV
                     MQDGFMKGLFGIETTGPILAFLPVITIGILFGLAMDYEVFLMSRIHEEYSKTGDNDYS
                     IKVGLKESGPVIVAAALIMFSVFFAFVFQEDVMIKSMGMALAFGVLFDAFVVRMMLIP
                     ALTKLFGKGSWYLPAWLNRIIPRVDIEGHALEKYKTVESQESEAKDSKETYDTTFKVY
                     PQGATNVSKYQDVHGQDDAHSIVLDDKTMALYQEVKQQSASSLFLYDALIDYQNKHQL
                     NSKQQVTNIEQLNKNIEKLNQLLEKNLRNKS"
     gene            19029..19577
                     /gene="farR"
                     /locus_tag="NBH32_000625"
     CDS             19029..19577
                     /gene="farR"
                     /locus_tag="NBH32_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid efflux pump transcriptional regulator
                     FarR"
                     /protein_id="HCD1514255.1"
                     /translation="MKETDLRVIKTKKALSSSLLQLLEQQLFQTITVNQICDNALVHR
                     TTFYKHFYDKYDLLEYLFNQLTKDYFARDISDRLNHPFQTISDTINNKEDLRDIAEFQ
                     EEDAEFNKVLKNVCIKIMHNDIKNNRDRIDIDSHIPDNLIFYIYDSLIEGFMHWIKDE
                     KIDWPGEEIDKIFHKIINIKIK"
     gene            complement(19654..19821)
                     /locus_tag="NBH32_000626"
     CDS             complement(19654..19821)
                     /locus_tag="NBH32_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514256.1"
                     /translation="MRYVISIIMAIVLAIWSLKQLSQSHLDSGFIFFFIVYVLCISYF
                     NSDKHDKNKKR"
     gene            complement(19933..21477)
                     /gene="pruA"
                     /locus_tag="NBH32_000627"
     CDS             complement(19933..21477)
                     /gene="pruA"
                     /locus_tag="NBH32_000627"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HCD1514257.1"
                     /translation="MVVEFKNEPGYGFSVQENVDMFKKALKDVEKELGQDIPLVINGE
                     KIFKDDKIKSINPADTSQVIANASKATKQDVEDAFKAANEAYKSWKTWSANDRAELML
                     RVSAIIRRRKAEIAAIMVYEAGKPWDEAVGDAAEGIDFIEYYARSMMDLAQGKPVLDR
                     EGEHNKYFYKSIGTGVTIPPWNFPFAIMAGTTLAPVVAGNTVLLKPAEDTPYIAYKLM
                     EILEEAGLPKGVVNFVPGDPKEIGDYLVDHKDTHFVTFTGSRATGTRIYERSAVVQEG
                     QNFLKRVIAEMGGKDAIVVDENIDTDMAAEAIVTSAFGFSGQKCSACSRAIVHKDVYD
                     EVLEKSIKLTKELTLGNTVDNTYMGPVINKKQFDKIKNYIEIGKEEGKLEQGGGTDDS
                     KGYFVEPTIISGLKSKDRIMQEEIFGPVVGFVKVNDFDEAIEVANDTDYGLTGAVITN
                     NREHWIKAVNEFDVGNLYLNRGCTSAVVGYHPFGGFKMSGTDAKTGSPDYLLHFLEQK
                     VVSEMF"
     gene            complement(21667..22266)
                     /locus_tag="NBH32_000628"
     CDS             complement(21667..22266)
                     /locus_tag="NBH32_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HCD1514258.1"
                     /translation="MTEKEKMLAEKWYDANFDQDLINERARAKDICFELNHTRPSDTI
                     KRKELIDQLFQTTTDNVSISIPFDTDYGWNVKLGKNVYVNTNCYFMDGGQITIGDNVF
                     IGPNCGFYTATHPLNFHHRNEGFEKAGPIHIGSNTWFGGHVAVLPGVTIGEGSVIGAG
                     SVVTKDIPPHSLAVGNPCKVVRKIDNDLPSETLNDETIK"
     gene            22505..22696
                     /gene="cwrA"
                     /locus_tag="NBH32_000629"
     CDS             22505..22696
                     /gene="cwrA"
                     /locus_tag="NBH32_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall inhibition responsive protein CwrA"
                     /protein_id="HCD1514259.1"
                     /translation="MRILITGTVAILIILGLVKTIQDYEMTNDTSRQMSDNKDDDKVI
                     HLNNFKNLHAKEFNPSDFF"
     gene            22936..25344
                     /locus_tag="NBH32_000630"
     CDS             22936..25344
                     /locus_tag="NBH32_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HCD1514260.1"
                     /translation="MANTKKTTLDITGMTCAACSNRIEKKLNKLDDVNAQVNLTTEKA
                     TVEYNPDQHDVQEFINTIQHLGYGVAVETVELDITGMTCAACSSRIEKVLNKMDGVQN
                     ATVNLTTEQAKVDYYPEETDADKLVTRIQKLGYDASIKDNNKDQTSRKAEALQHKLIK
                     LIISAILSLPLLMLMFVHLFNMHIPALFTNPWFQFILATPVQFIIGWQFYVGAYKNLR
                     NGGANMDVLVAVGTSAAYFYSIYEMVRWLNGSTTQPHLYFETSAVLITLILFGKYLEA
                     RAKSQTTNALGELLSLQAKEARILKDGNEVMIPLNEVHVGDTLIVKPGEKIPVDGKII
                     KGMTAIDESMLTGESIPVEKNVDDTVIGSTMNKNGTITMTATKVGGDTALANIIKVVE
                     EAQSSKAPIQRLADIISGYFVPIVVGIALLTFIVWITLVTPGTFEPALVASISVLVIA
                     CPCALGLATPTSIMVGTGRAAENGILFKGGEFVERTHLIDTIVLDKTGTITNGRPVVT
                     DYHGDNQTLQLLATAEKDSEHPLAEAIVNYAKEKQLTLTETTTFKAVPGHGIEATIDH
                     HHILVGNRKLMADNDISLPKHISDDLTHYERDGKTAMLIAVNYSLTGIIAVADTVKDH
                     AKDAIKQLHDMGIEVAMLTGDNKNTAQAIAKQVGIDTVIADILPEEKAAQIAKLQQQG
                     KKVAMVGDGVNDAPALVKADIGIAIGTGTEVAIEAADITILGGDLMLIPKAIYASKAT
                     IRNIRQNLFWAFGYNIAGIPIAALGLLAPWVAGAAMALSSVSVVTNALRLKKMRLEPR
                     RKDA"
     gene            25624..25830
                     /gene="copZ"
                     /locus_tag="NBH32_000631"
     CDS             25624..25830
                     /gene="copZ"
                     /locus_tag="NBH32_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HCD1514261.1"
                     /translation="MSQEILNVEGMSCGHCKSAVESALNNIDGVTSADVNLENGQVSV
                     QYDDSKVAVSQMKDAIEDQGYDVV"
     gene            complement(25920..26918)
                     /locus_tag="NBH32_000632"
     CDS             complement(25920..26918)
                     /locus_tag="NBH32_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1514262.1"
                     /translation="MTKIKIMSVRDEDMPYIKAWAEKHHVEVDITKEALTDDNVEGVA
                     GYDGLSLSQQIPLSEHVYKRLNELGIKQIAQRSAGFDTYDLELANKYNLIVSNVPSYS
                     PNSIAEFAVNQAINVVRHFNQIQTKVREHDFRWEPTILSKSIKDLKVAVIGTGRIGRV
                     VADIFANGYQSDVVAYDPFPNAKIATYVDYKDTIEEAVEGADIVTLHVPATKYNHYLF
                     NAELFKHFKKGAVFVNCARGSLVDTKALLEALDNGVIKGAALDTYEFERKLFPSDQRG
                     KTLNDPLLESLIDREDVILTPHIAFYTEAAVENLIVDALDATLDVLQTGDTRLRVN"
     gene            complement(26941..28095)
                     /locus_tag="NBH32_000633"
     CDS             complement(26941..28095)
                     /locus_tag="NBH32_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1514263.1"
                     /translation="MISNKLANIPDSYFGKTMGRKIEHGPLPLINMAVGIPDGPTPQG
                     IIDHFQQALTIPENQKYGAFHGKEAFKQAIVDFYQRQYNVTLDKEDEVCILYGTKNGL
                     VAVPTCVINPGDYVLLPDPGYTDYLAGVLLADGKPVPLNLEPPHYLPDWSKVDSQIID
                     KTKLIYLTYPNNPTGSTATKEVFDEAIAKFKDTDTKIVHDFAYGAFGFDAKNPSILAS
                     ENGKDVAIEIYSLSKGYNMSGFRVGFAVGNKDMIQALKKYQTHTNAGMFGALQDAAIY
                     ALNHYDDFLEEQSNIFKARRDRFEAMLAKADLPFVHAKGGIYVWLETPPGYDSEQFEQ
                     FLVKEKSILVAPGKPFGENGNRYVRISLALDDQKLDEAAIRLTELAYLYE"
     gene            complement(28522..30030)
                     /locus_tag="NBH32_000634"
     CDS             complement(28522..30030)
                     /locus_tag="NBH32_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1514264.1"
                     /translation="MKIAVIGAGVTGLAAAARIASQGHEVTIFEKNTNVGGRMNQLKK
                     DGFTFDMGPTIVMMPDVYKDVFTMCGKNYEDYIELRQLRYIYDVYFDRDDRITVPTDL
                     AELQHMLESIEPGSTHGFMSFLTDVYKKYEIARRYFLERTYRKPSDFYNMTSLVQGAK
                     LKTLNHADQLIEHYIDNEKIQKLLAFQTLYIGIDPKRGPSLYSIIPMIEMMFGVHFIK
                     GGMYGMAQGLAQLNKDLGVNIELNAEIEQIIIDPKFKRADAIKVNGDIRKFDKILCTA
                     DFPSVAESLMPDFAPIKKYPPHKIADLDYSCSAFLMYIGIDIDVTDQVRLHNVIFSDD
                     FRGNIEEIFEGRLSYDPSIYVYVPAVADKSLAPEGKTGIYVLMPTPELKTGSGIDWSD
                     EALTQQIKEIIYRKLAAIEVFEDIKSHIVSETIFTPNDFEQTYHAKFGSAFGLMPTLA
                     QSNYYRPQNVSRDYKDLYFAGASTHPGAGVPIVLTSAKITVDEMIKDIERGV"
     gene            complement(30042..30905)
                     /locus_tag="NBH32_000635"
     CDS             complement(30042..30905)
                     /locus_tag="NBH32_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HCD1514265.1"
                     /translation="MTMMAMNFKYCHKIMKKHSKSFSYAFDLLPEDQRKAVWAIYAVC
                     RKIDDSIDVYGDIQFLNQIKEDIQSIEKYPYEHHHFQSDRRIMMALQHVAQHKNIAFQ
                     SFYNLIDTVYKDQQFTMFETDAELFGYCYGVAGTVGEVLTPILSDHETHQTYDVARRL
                     GESLQLINILRDVGEDFENERIYFSKQRLKQYEVDIAEVYQNGVNNHYIDLWEYYAAI
                     AEKDFRDVMDQIKVFSIEAQPIIELAARIYIEILDEVRQANYTLHERVFVDKRKKAKL
                     FHEINSKYHRI"
     gene            complement(30942..32069)
                     /gene="crtQ"
                     /locus_tag="NBH32_000636"
     CDS             complement(30942..32069)
                     /gene="crtQ"
                     /locus_tag="NBH32_000636"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,4'-diaponeurosporenoate glycosyltransferase"
                     /protein_id="HCD1514266.1"
                     /translation="MKWLSRILTVIVTMSMACGALIFNRRHQLKAKTLNFNHKSLTII
                     IPARNEEKRIGHLLHSIIQQQVPVDVIVMNDGSTDETARVARSYGATVVDVVDDADGK
                     WYGKSHACYQGVTHACTNRIAFVDADVTFLRKDAVETLINQYQLQDEKGLLSVQPYHI
                     TKRFYEGFSAIFNLMTVVGMNVFSTLDDGRTNQHAFGPVTLTNKEDYYATGGHKSANR
                     HIIEGFALGSAYTSQSLPVTVYEGFPFVAFRMYQEGFQSLQEGWTKHLSTGADGTKPK
                     IMAAIVLWLFGSIASILGLCLSLKYRQMSVGKMLTVYLSYTTQFIHLHRRVGQFSNLL
                     MVCHPLLFMFFTKIFIQSWKQTHRYGVVEWKGRQYSISKEQ"
     gene            complement(32075..33568)
                     /locus_tag="NBH32_000637"
     CDS             complement(32075..33568)
                     /locus_tag="NBH32_000637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1514267.1"
                     /translation="MTKHIIVIGGGLGGISAAIRMAQSGYSVSLYEQNNHIGGKVNRH
                     ESDGFGFDLGPSILTMPYIFEKIFEYSNKQMSDYVTIKRLPHQWRSFFPDGTTIDLYE
                     GIKETGQHNVILSKQDIEELQNYLNYTRRIDRITEKGYFNYGLDTLSQIIKFHGPLNA
                     LINYDYVHTMQQAIDKRISNPYLRQMLGYFIKYVGSSSYDAPAVLSMLFHMQQEQGLW
                     YVEGGIHHLANALEKLAREEGVTIHTGARVDNIKTYQRRVTGVRLDTGEFVKADYIIS
                     NMEVIPTYKYLIHLDTQRLNKLEREFEPASSGYVMHLGVACQYPQLAHHNFFFTENAY
                     LNYQQVFHEKVLPDDPTIYLVNTNKTDHTQAPVGYENIKVLPHIPYIQDQPFTTEDYA
                     KFRDKILDKLEKMGLTDLRKHIIYEDVWTPEDIEKNYRSNRGAIYGVVADKKKNKGFK
                     FPKESQYFENLYFVGGSVNPGGGMPMVTLSGQQVADKINAREAKNRK"
     gene            complement(33561..34049)
                     /gene="crtO"
                     /locus_tag="NBH32_000638"
     CDS             complement(33561..34049)
                     /gene="crtO"
                     /locus_tag="NBH32_000638"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl-4,4'-diaponeurosporenoate
                     acyltransferase"
                     /protein_id="HCD1514268.1"
                     /translation="MKKYIKTAFFCSMYWLIVQLNIANLGTRIPDKYFRQKHIIFKSF
                     NFEKHGKFWNKWFYVRKWKHKILDGHQLNQNIYDQRHLMTINTDEIEKMIIETKRTEL
                     IHWISILPVIIFNKGPRLVKYINIFYAMITNVPIIIVQRYNRPRLTQLLRILKRRGER
                     HD"
     gene            complement(34224..34991)
                     /locus_tag="NBH32_000639"
     CDS             complement(34224..34991)
                     /locus_tag="NBH32_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1514269.1"
                     /translation="MKKIVTATIATAGLATIAFAGHDAQAAEQNNNGYNSNDAQSYSY
                     TYTIDAQGNYHYTWTGNWNPSQLTQNNTYYYNNYNTYSYNNASYNNYYNHSYQYNNYT
                     NNSQTATNNYYTGGSGASYSTTSNNVHVTTTAAPSSNGRSISNGYASGSNLYTSGQCT
                     YYVFDRVGGKIGSTWGNASNWASAAASSGYTVNNTPKAGAIMQTTQGYYGHVAYVEGV
                     NSNGSVRVSEMNYGHGAGVVTSRTISANQAGSYNFIH"
     gene            complement(35352..37163)
                     /locus_tag="NBH32_000640"
     CDS             complement(35352..37163)
                     /locus_tag="NBH32_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCD1514270.1"
                     /translation="MDTKDFKRLEKMYSPRYLPGLDGLRAFAVIGIIIYHLNAQWLSG
                     GFLGVDTFFVISGYLITSLLISEYYRTQKIDLLEFWKRRLKRLIPAVLFLICVVLTFT
                     LIFKPELIIQMKRDAIAAIFYVSNWWYISQNVDYFNQFAIEPLKHLWSLAIEEQFYLL
                     FPLVITFLLHRFKPRNIIQTLFIVSLISLGLMIVIHFITGDNSRVYFGTDTRLQTLLL
                     GCILAFIWPPFALKKDISKKIVVSLDIIGISGFAVLMTLFFIVGDQDQWIYNGGFYII
                     SFATLFIIAIAVHPSSLFAKFLSMKPLLIIGKRSYSLYLWHYPIIVFVNSYYVQGQIP
                     VYVYVIEIVLTALMAEISYRFIETPIRKKGFKAFAFLPKKKGQFARTVLVILLLVPSI
                     VVLSGQFDALGKQHEAEKKEKKTEFKTTKKKVVKKDKQEDKQTANSKEDIKKSSPLLI
                     GDSVMVDIGNVFTKKIPNAQIDGKVGRQLVDATPIVKSQYKDYAKKGQKVVVELGTNG
                     AFTKDQLNELLDSFGKADIYLVSIRVPRDYEGRINKLIYEAAEKRSNVHLVDWYKASA
                     GHPEYFAYDGIHLEYAGSKALTDLIVKTMETHATNKK"
BASE COUNT        11970 a         7188 c         5705 g        12511 t
ORIGIN      
        1 tttctatcaa ataattaata gaaatcagct tttttacatt gcctaagaac ttaatgtctc
       61 aagctctttt gtaacgtatt ctgtttcaac tcgctacttc gcatacgcac tacgaatgac
      121 aaaaagttca ttcatttgat atgctgttta atttttaatc attatttgac tgtcataatt
      181 agcttacctg gatcagcatc ttgtacatct tcaatgacaa gttctttatt ttcaagattt
      241 gtaataatca ttggtgtaac gatgcttggt gcgtgtgatt tcaagtatgc taaattcact
      301 ttcattaatg gttgaccttg tgttactttt tcatcaacgt taatcagact ttcgaatcct
      361 tcgccattca gtttcactgt atcgatacca atatgaataa gtacttcgac accactttca
      421 gattctaatc ctatcgcatg tttcgtaggg aagattgttt tcactgtacc atcaaacggt
      481 gctacaattt cacctttttc agggataaag ccaacaccat cacccatcat tttaccagcg
      541 aatacttgat caggtacttc tgataatgga atgatttgac cgacacctgg tgcatagata
      601 tctgttgttc caagtgcttc tacgtgaact ggttcatctg acatttcttc agtcactgtc
      661 gtttcacttg gtttcgtaat ttcaccactc ataatcttgg ccatatcatg tttaatttga
      721 tctgattttg gaccaaagat agcttgcata ttgtttccaa cttctaatac acctgatgcg
      781 cctaaagctt taatacctgc tacatctact tttgatttat caaccacttc tacgcgcaga
      841 cgtgtaatac atgcatctaa atgtttaatg ttttcttttc cacccattgc atctaagaca
      901 tcaaatggta attttgcgac actagagtta cgaatttcag tttcttcatc ttcacgacct
      961 ggtgttttca atttaaactt acgaattgca aagtcgaata agaagtaata cacgatagca
     1021 tatacaatac cgactggaat aactaataat gcgtgtgaac gatcccagtt taataaacca
     1081 tataaaatat aatctatgaa accacctgag aatgtcatac caattttaac gcctaataaa
     1141 tgcattacta agaatgatgt accagctaat aatacgtgaa ttccataaag tactggtgct
     1201 acaaataaga atgaaaattc taatggctca gtgataccag ttaaaaatgc agttaatcct
     1261 gctgataaca ttaaaccacc cacgactttt ttacgttctg gtcgtgcatt tttataaatc
     1321 gcaaatgctg ccgctggtaa accaaacatc ataaatggat atttaccagt agtaaatgca
     1381 ccagcagtaa atggtacgcc atctttcaat tgtgccatcc aaatacgttg gtcaccacga
     1441 actaattcac ctgcgtgatt tgtataacta ccgaattcaa accagaacgg tgaatagaaa
     1501 atatgatgta aaccaaatgg aattaatgag cgttcaataa taccgaatat aaacgttgtt
     1561 aatgttaaat ttttatttaa taagaaattc gataaactat ttaatccgtc ttgaattggt
     1621 ggccacgcaa agcttaaaac cacacctgtt gcaatcgcta cgaccgatgt cacaatcggt
     1681 acaaatcgtt tacctgcaaa gaatcctaaa aatggtggta gtgtaatgtt ataaaatttg
     1741 ttgtagcacc atgcggctaa agcacccatg ataataccgc caaacacacc cgtttgtaat
     1801 gttggaatac ctaatactaa ggcatgtgcc ggttctttcg ctgcttgact taattctttt
     1861 gcccctttgg catatgagaa aatgtcatca attgtaatgt gcaacacttt ccccattgtt
     1921 gcattcataa ttaagtaacc tactagcgct gctaatgctg caacaccgtc tcctcctgct
     1981 aatccaagtg ctgtaccaac tgcaaataat aatggcaagt tatcaaatac aacttgtcct
     2041 gctgcttcca tgaccgacga aatcattaca atgatatcgt tttttaacca tggtgcaatt
     2101 tctactaatt gttcgttgtg cattgcgtta ccaaacgcta ataaaatacc agctgctggt
     2161 aaaatcgcaa caggtaacat taatgcttta ccgatacgtt gcaattgtcc aaacaatttc
     2221 ttaaacatat aaacatcccc tatctcaatt tccgcttcca tgtacaaaaa aagcaggcat
     2281 gaacaaacaa gactattctc atagtgcggt tgtttgctca tgcctgcatt accagtaaca
     2341 cgcaagtgat atctaattca tagctattat acatatttgt aaaagcgttt tcaacaactt
     2401 tttcacaatt ttatcatcaa ttcagtacga tcatttgtga taaataaaca aaacggcaat
     2461 tcccattttg ttgaaataga aattaccgtt tttatagttc aattcaattg tgataacctt
     2521 taaatcagtt ataagaaacg ttttgctgcc actgaaattg gtggaatttg atctaaatct
     2581 aaatgcttat cttcagtaat tgatctaaat gcccacttac cataaccaag cgcttcttca
     2641 tttacaattt tacctggtaa tggcgcagca ttaggatcaa catatacatc tacaatcgtt
     2701 ggtacatctt gtgctaatgc ctcttcgact atagcatcta cttcgctagc actcttaatt
     2761 gtataacctt taccacctgc tgcctcagca aattttgcat gatccatatc agaaaaatca
     2821 actgcatatt ctaattcacc agctgcttgt tgttcatatt taataaatgc taactgtttg
     2881 ttattaagta caaatacagt taaaggtaaa tcatattgta ctgctgtagc gaagtcttgc
     2941 attaccattt ggaatgcacc gtcaccagca atggcaatcg cttgtctatt tggatatgca
     3001 atttttgatg ccatagctcc tggaagaccg caacccattg tacctaacca acttgaaatg
     3061 atgaacttgt tgttcacacc aagatttaaa taacgtgtag accaaactgt tgcagtacca
     3121 acatctgctg aaattactgc atcatcttta ataaatttgt taattgatgc cattaaacgt
     3181 tctggacgta atggtttgct attattattt ttatcttgtt ccatccattt atcccaaacc
     3241 gctttacgtt ctaacgtttt gtttaaaaat ggtctttccg caacatgttt aatattttca
     3301 gttaactgat gcaatgcaat tttactgtct ccaacaattc ctacattaat attaaaacga
     3361 tgtccgatat ttttaggatt tgtgtcgatt tggattgctt taatattttt cttaggtaag
     3421 taatctacat atggatagtt tgtacctacc ataattagta aatctgcatc ctgcattgtc
     3481 tgatatgagg tttttgtacc tattttacct aagttaccaa tactgtatgg atggtcatcc
     3541 ggtaagattg tcttagcagg taatgaatga acgaccggaa tttttgccgc ttcaacaaac
     3601 gcacgtagct catctttcgc atgttttgca ccaacaccaa tcaacataac cggcttttta
     3661 ctcttattga ttaatttaac agctttttta atgtctttat attttggtga tacaacagtt
     3721 ggtcttgatg tatctactgg tttattcgtt gtatctttaa ttttttcagt taataagtcg
     3781 ttaggacaaa taacaacagc tacacctttt tgttcatatg ccgtacgaat tgcttcgtta
     3841 acaatttcaa acacattgtc acctttttca atttggtgat tataaatggc tacatcttca
     3901 cataattttt gtaaatttgt ttcttggaat gctttcgttc caagtgctgt actattcgtt
     3961 tgtccagata atattaattg cggtacatta tccattttgg catcatacat accatttaat
     4021 aaatgaatta aaccagggcc accgatactt aatgccacac cgattttacc agttaatttt
     4081 gtgtaaccag cagccgctaa gcttgctact tcttcatgac gtacatgata aaatttaaat
     4141 tgatctctca ctgtacgtaa actatcgact actgcgtcga ttgagtctcc tggaatacca
     4201 tacaagtgat ctatatccca tgcttgtaat gctttaacta atgcttcatt tgcttttatt
     4261 tttgccatta ttactaatag cctccctttc tgtctatcat tttaaatatc taaattaata
     4321 gaataataaa attagaacag gtattaacac tgaactaata actgcagtta aaatcatccc
     4381 aattgaacta aatgcaccgg attcaatatc catttctagt gctttagctg tgccaaatgc
     4441 atgtgacgca ttcccatacg ttaaaccttt ggcgatagaa gattcaaatc ttccaaatct
     4501 taataacatc gaacctaaaa tactaccgat taaacccgtt gtgataataa aaagtacggt
     4561 catcgtatct gtaccaccta gttcatgtga cacttcgata cctactgcgg ctgttataga
     4621 tcggggcaat aacgttacaa tgacgtcttt agaatagcca aatgctttaa gtgttaagaa
     4681 cactaacatg aaattcagca tgacgccagt taatacactt gcaaaaatga tagagacatt
     4741 gtctttaatt ttctctctat ttttataaag tgggtacgct aaacatacga ccgttgcgtt
     4801 taaaatatgg ttgatccaac tgccaccttt catatacccg ttataactaa ttccaaagat
     4861 aagtaagaca aaaataattc ctaaagatgc aattaatgct ggattcaaaa atgggttcgg
     4921 atatttttgt tgtaaccttt tagcgaaata atataaaacg acagtcaaca aaatcattaa
     4981 taaggcttgc acgtaatcat tcataagcgt ctacaccttt acgatgttta tgtttaacag
     5041 acattttttc agcaatataa cctgaagata atgcaacgat acatgttcct atgataatga
     5101 ctgcgaaaaa gagtatataa tttagcgtaa tttcggaagc aacatccata atacctacaa
     5161 ctgacggtat gaagaaaaag accatcgtct ttaataaaaa gtttgcaccg tcttctaccc
     5221 aagttagcgg tacaatctta aattgtaata gtaaataaaa taaaaataga ccaacaatac
     5281 tgccggctaa gggaagatga aaaatctttt gaatttctgt gccaatataa gtaatcacaa
     5341 tgatgattcc tagttgtagt agtagtttga ttattaattg gactttgtgc atggcgccat
     5401 ccctttctaa atatgtctaa attgttacaa taactattat aaagatggcg tacacaatat
     5461 aaaaatactc tttttttata gtttgtatta ctattttcta tggaaacgct ctctaaattt
     5521 cacttcattt tcacaaaaaa acactcatta tttcaatgac ccacgtaatg atgcgcctta
     5581 aataatgagt gctttattaa tatgttagcc taaacggtct ttcaaaaatt ctatccattt
     5641 cgttgtggca tgacttaatt gtttatcctt cttccaaaca acacctaatt cccaatgtac
     5701 atgagcatct tcaatgcgta gcagcttcac atctccattt agttgctctg aaattgatgt
     5761 tggtaaaata ctaataccta attcattcgt aactaaatct tctataacat gccattgtga
     5821 aatttgcgct acagtattcg gaacaaagcc aacgttttta gcattttcaa taattttatc
     5881 attcaagtaa aagtctttat taaataaaat gaagtcttca cctgctaaat cttcgagttt
     5941 aacagtttca tattttgcga gtcgatgctc tctgctcacg ataagtcgca aatcttcctt
     6001 atctagggta gtataatcga aaatatgatg atcgactggc aaagtggtca cgcctatatc
     6061 tacttcatca ttgataattt gctgttcaat cgttttgccg ccattttcta ttaaattata
     6121 tgtaacattt ggatattgtt gatggaatgc accaagaata ttgataagaa tcttcatatt
     6181 catgactgcc gacataccca tgtttatatg ccctgtttcc agtccattca agcgttccat
     6241 ttcagatggt aaataatcat acagtgcaac aatttcttta cttttctcat aaaaaatttg
     6301 acctgcatcg gtaagaatta aatgtctttt acttctatca aataagggcg tgcccatttc
     6361 attttcaata tctttaattg ctttactaat tgtaggttgt gcaatatata atgatttgga
     6421 agcattagtc atgccccctt gcttaacgac ttcaataaaa tatttcatat gtttgatatc
     6481 cactaacatc atcctaacta tttcaacacg tttatttcat tatacacaac ttcttaaaca
     6541 ttcaataaat ttagatttat aaaattgctt tataaaaagt ttcatgtaaa atacacattc
     6601 ttttatggta ttatattaat ggctcaagtt tgttattcga ggtgaggtat tgagctttta
     6661 cgaatttatg caaagttttc ttggtgatga cacaccatta ggtgaattgg ttgattggat
     6721 taatcaagat agcaatttcc ctagagaagt gaagagccat agtgaaatat tgtcatattt
     6781 tcgaacaaat ccatgtcctg aaagcatctc agtacctgta attaaacggg cactatcagt
     6841 tttcaaccaa ttcacaaatg tacaatgata catgaataca tggagtcaca tcatctcatg
     6901 ttagcgctct gattgcaggg cgctattttt aatgttatcg ctacatttag tatgttgtga
     6961 ttgctatttt ctttcagtta taaaaccacg taccttttct tttaaatcat ttatattttc
     7021 ccactaatat ccgctgttaa tcaattctga catccttgta tcactatgac aattaattgt
     7081 taaatacatt aatttctaca ttttatgaaa aaatccattt ttattacaat tcaacacttt
     7141 atatgacaac ttcattacag ttacttttat tgttgattgc ttgcattgtt ttctaaaaaa
     7201 attttgttat cataaataac gttgaataaa gaaaaaaatt aacttgggag ataaaaatgg
     7261 aatataaaaa gatactaatt cgtttattaa ttgcttttgc agtacttttc tcagcagatt
     7321 tcacttatca atccgtcgaa caaacgcatc aatcgcatgc cgcagttaat tattatagta
     7381 aaaaccaatg tacatggtgg gcatttaaac gtcgcgcaca agttggcaaa cctgtctcta
     7441 acaaatgggg taatgccaaa aattggtata acaatgcacg taaatcaaaa tatgcgactg
     7501 gtcgtacacc aagaaaattt gctgtaatgc aatcaactgc aggatattat ggacatgtcg
     7561 cagttgttga acaagtatat aaaaacggta gtattaaagt ttcagaatac aacttttatc
     7621 gcccattaaa atacaataca cgtgtactaa gcaaaaaggc agcacgtaac tttaactata
     7681 tttactaatc aaaaaacttc tatcacgaac gcttcaattt cctgtatgcg tgtgatagaa
     7741 gtttttattt tttgttcata ttaatttatt taagcgctac gatggactga cttaaacttt
     7801 tctttaaaac cgctattcgc aatttactat tgttgtctaa tttcttgtaa aatacgttcc
     7861 gctgcttgtg tatttgcacg gggttcttgc ttcaatgctt cagctacttt agcaatttca
     7921 tcaccttttg ctcctacaac gatagctaat gatttatatt gtaaactcat atgaccttgt
     7981 tgaatacctt ctgacacaag cgcgcgacat gctgcaaagt tttgcgctaa accaacggca
     8041 gcaactacat gacctaattc ttgtgctgac tctacattta gtagctctaa tgaagcttta
     8101 gctattggta atactttcgt accaccccca acaattgcta atgtcattgg cacttcaatt
     8161 gtaccaatca atcgttgacg atcttgatcg taacgccatg tagcaatacc acgatactgt
     8221 ccgtcacgac tcgcatatgc atgcgcactt gcttctgcac cacgcgtatc atttcctgtt
     8281 gctaaaacaa cagcatgtat gccattcata acacctttat tatgtgttgc agcacgatga
     8341 atatcaactt gtgccaatac agaagcacgt tccattcgtt tggcaacctc ttccccagtt
     8401 ctctcgcccc ttgctaaatc tttaacatca atttcgcctt gaactttaac aacggacgct
     8461 gttgcatgat tggataaaat actcattaaa atatcgcttt gcggaaattc attttttaaa
     8521 aatgcagtta tggcctctaa aatcgtatta agcatattag cgcccatagc atctttcgta
     8581 tcaacaaata cttttaaaga tagtaactgt tgctcaggaa atgtatcaat cgctatacgt
     8641 tggtaaccac caccacgcgc tttaatagaa ggatatgcct catccgcaat tttatgaatt
     8701 tgcttttcta aagctttaat gtctgctgat aatttttcag tatcgtcaac gccatcaaag
     8761 acgatttgac ctatcataat acgttcagaa gataccgttt taaatccgcc agtctgattc
     8821 actagctttg caccataact agctgcagcg acaactgaag gctcttccac catcataggt
     8881 acaacatatg ccttatcgtc cacaatgata ttcggtaata atccaacggg taatgcacct
     8941 tgcgcgatga cattttcaat taaactattg gctacttctt catcgattaa tggatgattc
     9001 agtaaaatgt cgaattgttc ttctgataac cattgcttat caaccaattg ttgtaacttt
     9061 tctttacgag ataaatgtcg aaaattctta tctaaatttt gcatggacgt actcctttta
     9121 cttcacataa tttttaacat ttcaatcact actattttta ccacaaaata acgtcattcg
     9181 tcttaaaatt caattgaata attgtcgttt tgactttaaa ataaaacaag gtaaattaaa
     9241 acgcttacaa gaaacgacaa atcattttta aatttagtat atttctttgt ataaaattag
     9301 catattctga tatgatacaa gtgttgcttt tataaatttg aaaggatgta aaaccttatg
     9361 acaataggta tcgataaaat aaacttttac gttccaaagt actatgtaga catggctaaa
     9421 ttagcagaag cacgccaagt agatccaaac aaatttttaa ttggtattgg tcaaactgaa
     9481 atggctgtta gtcctgtaaa ccaagacatc gtttcaatgg gcgctaatgc tgctaaggac
     9541 attataacag acgaagacaa aaagaaaatt ggtatggtaa ttgtggcaac tgaatcagca
     9601 gttgatgctg ctaaagcagc cgctgttcaa attcacaact tattaggtat tcaacctttt
     9661 gcacgttgct ttgaaatgaa agaagcttgt tatgctgcaa caccagcaat tcaattagct
     9721 aaagattatt tagcaactag accgaatgaa aaagtattag ttattgctac agatacagca
     9781 cgttatggat tgaattcagg cggcgagcca acacaaggtg ctggcgcagt tgcgatggtt
     9841 attgcacata atccaagcat tttggcatta aatcaagatg ctgttgctta cactgaagac
     9901 gtttatgatt tctggcgtcc aactggacat aaatatccat tagttgatgg tgcattatct
     9961 aaagatgctt atatccgctc attccaacaa agctggaatg aatacgcaaa acgtcaaggt
    10021 aagtcgctag ctgacttcgc atctctatgc ttccatgttc catttacaaa aatgggtaaa
    10081 aaggcattag agtcaatcat tgataacgct gatgaaacaa ctcaagagcg tttacgttca
    10141 ggatatgaag atgctgtaga ttataaccgt tatgtcggta acatttatac tggatcacta
    10201 tatttaagcc taatttcatt acttgaaaat cgtgatttac aagctggtga aacaatcggt
    10261 ttattcagtt atggctcagg ttcagttggt gaattctata gtgcgacatt agttgaaggc
    10321 tacaaagatc atttagatca ggctgctcac aaagcattat taaataaccg tactgaagta
    10381 tctgttgatg catatgaaac attcttcaaa cgttttgatg acgtagactt tgacgaagaa
    10441 caagatgctg ttcatgaaga tcgccatatt ttctacttat caaatattga aaataacgtt
    10501 cgcgaatatc acagaccaga gtaatctgtg tatttgaaag acatttaaat ataacctaaa
    10561 aacagcagta agaccacttc taattgaaat cgtcttactg ctgttttcta tttataatgc
    10621 ttcgtcttga atgaattcat tatgcctatt tgacatatta ttaaagtttt cctaatgcct
    10681 ggatccttta tacgttacgg cttcgtgcta tgttttggta cataaagctt tgacatatcg
    10741 atattctcca actctaacag cttaatttta ttattaatcg ttccaccgaa tcctgttaag
    10801 ctacccgttt taccgacaac acgatgacat ggcacgataa ttgataatgg attacttccg
    10861 actgcacctc caaccgcttg agctgacatt tttggcttat caagcacctt gcctactttt
    10921 ttggcaatag caccatacgt tgttaacgtc ccataaggaa tctgtcttaa ttcattccaa
    10981 acacactgtt gaaaatcact acctgttggc tttaaaggta ttgtgatttc aggattgtca
    11041 cctttaaaat acgcgtctaa ccactgtgtc gcctctttaa atatcgctaa agacgtattt
    11101 tcttccctat taccatcacc ttgttgattt tcaaacaaaa cagcggtcag acttacccca
    11161 tcactcaaaa gttccaatcg tccaacagga gaatcatagt aactcttata taccataaac
    11221 acacactcct tttgcatcat catactattc atttaacttt atgaaaatga ttaacacttt
    11281 atgctttatc aagatcaact attcacttcc aaatcaaaat tcacacagac ttccaacaca
    11341 atataccatt tcttaattaa ctatgtatca acacatacat ttcttaacca ttgattaata
    11401 tttactttta aattcttact tacttcaaaa attacacaaa aagttcataa agtgcctaca
    11461 atgcgccgtt tttaaaacta caatggtgga aaaatagaat tgccattgtt tatagtttgc
    11521 atatagtttc aatgtatttt cttttactta ttagtttaaa aatttagagg tgatgtttat
    11581 gaataacaat tttttcaata gtgacttcga ttctatttta cgaagaatga tgcaagacat
    11641 gcaaagctca aatcagacta gtaacaagaa atactatatt aatggtaaag aagtttcacc
    11701 ggaagaatta gcacaattta cgcaacaggg tggtaatcat tctgccgaac aaagtgcgca
    11761 agcttttcaa caagcagcac taagacaaca aggtcaacaa ggtcaacaag gtggcaatgg
    11821 caattattta gaacaaattg gtcgcaatct tacgcaagag gctcgtgacg gattattaga
    11881 tccagtcatc ggtcgtgata aagaaatcca agagactgca gaagtcttaa gtagacgcac
    11941 taaaaacaat cctatcctag tcggagaagc tggggttggt aaaactgcga ttgtcgaagg
    12001 tttagcgcaa gcaatcgttg aagggaacgt acctgcagca atcaaagata aagaaattat
    12061 atctatagat atttcatcgc ttgaagctgg aacgcaatat cgtggcgcct ttgaagaaaa
    12121 catccaaaag ttagttgaag gcgttaaatc atcacaaaat gctgtgctct tctttgacga
    12181 aattcaccaa atcatcggtt ccggtgcaac tggaagtgat tccggtagta aagggttatc
    12241 tgatatttta aaaccagcat taagtcgtgg tgaaatttca attataggtg caacgactca
    12301 agatgaatat cgaaacaata ttcttaaaga tgctgcatta acgcgcagat ttaatgaagt
    12361 acttgttaat gaaccaagtg ctaaagatac tgttgaaatt ttaaaaggta ttcgcgaaaa
    12421 attcgaagaa caccatcaag taaaattacc agatgacgta ttaaaagcat gtgttgactt
    12481 atcaattcaa tatattccac aacgattatt accagataaa gcaatcgatg tgttagatat
    12541 tacagcagca catttatctg cacaaagccc tgctattgat aaagtcgcga ctgaaaaacg
    12601 tatttctgaa ttggaacatg ataagcgtaa agcggtaagt gctgaagaat ataaaaaagc
    12661 tgacgacatt caaaatgaaa tcaaatcatt acaagataaa ttagaaaata gtaatggtga
    12721 acatactgcc gttgctactg ttcatgatat ttcagatact attcaacgat taactggtat
    12781 tccagtttct caaatggatg ataacgatat tgaacgttta aaaaatattt ctaatcgttt
    12841 aagaagtaaa atcataggtc aagatcaagc tgtagaaatg gtttctcgtg caattcgccg
    12901 taatcgtgct gggtttgatg acggcaaccg tccaattggc agtttcctat ttgttggccc
    12961 tactggtgtt ggtaaaacag agcttgctaa acaattagca attgatctat ttggtaataa
    13021 agatgcactg attcgacttg atatgagtga atatagtgac acaacagctg tttcaaaaat
    13081 gattggtaca actgctggtt atgtcggtta tgatgacaat tcaaatacgt taactgaaaa
    13141 agtacgccgt aatccatatt cagtcattct atttgatgaa atcgaaaaag caaatccaca
    13201 aattttaaca ttgttattac aagtaatgga tgatggtaat ttgactgatg gtcaaggtaa
    13261 tgtcatcaac tttaaaaata caattattat ttgtacatca aatgctggct ttggtaacgg
    13321 tacagaccaa gaatacgatg acattatgca tgaaatgaaa aaattcttcc gccctgaatt
    13381 ccttaaccgc ttcaacggta tcgttgaatt cttacattta gataaagatg cattacaaga
    13441 tatcgtcaac ttattattag acgatgtcca agtaacatta gataaaaaag gtattacgat
    13501 gaatgtctct caagatgcaa aagattggtt aattgaagaa ggttatgatg aagacttagg
    13561 cgcacgccca ttaagacgta ttgttgaaca gcaagtacgt gacaaaatta cagattacta
    13621 tttagatcat actgacgtta aacatgtgga tttagatatc gaggacaatg aattagtcgt
    13681 caaaggtaaa tcatgatact ttaacatatt gcgtatcaaa aaatgagcat caggacgccc
    13741 tcacctgtgc tcatttttta aattatttcc caggaaaatg attcgctgtg tgctgttctg
    13801 ttccacaaca atcacgatca atgtcacatg taccacattt tccttgtttc gaacgtttga
    13861 aaaatttaac taacgtatac aaggcatatc cgaaaattgc taaaaaaatt aaaatgttaa
    13921 taatgactga cactttaacc actccttaaa caaataaatg tccgacttga taaaaaatga
    13981 atgttaagac atatgcagtg actagaggat aggcaactgc aagtgccgtc catttccatg
    14041 aataagtctc tttacggatt gctgccactg tagaaacaca aggaatatac aatagtataa
    14101 atatcataaa tgcatacgca gatagcggtg tgaattgatt ttgaatcaca ttaacaaggc
    14161 ctgcatcacc tgatgaatag ataatcgcca tcgaacttac gataacttct tttgctaaaa
    14221 atcctggcac taacgtagca cctgcttgcc atgttccaaa tccgagcggt tgcactaaca
    14281 taccaaagaa gctaccaacc atatgtaaaa aactttgatt gatattcaca ttgataccat
    14341 gtggtcctac ataacttaat agccaaatga ctactgagcc gccaaaaata aatgtacctg
    14401 ctttacgaac aaagccctta gccttttccc aagtactacg ccacaacgtt ttaatggaag
    14461 gcacacggta tgttggcaat tccacaataa agattgcatt atcatttttc aaaaccgttt
    14521 tagtaagtac tgtactgact aaaaatgcca taataatacc taaaacatac aagcttaata
    14581 ctactaaaga ttgattctct ttgaaaaaga tacctacgaa caacgcatac actggcagtc
    14641 tagcagagca tgacatgaat ggtgcaatta atatcgttgt taaacgctct ttttcatttt
    14701 caatactgcg cgcagccata atactcggta cgttacaacc aaatccgata atcatcggta
    14761 taaaagactt cccgcttaaa ccgaacgatt ccataatacg atccattaaa accgcaatac
    14821 gtgccatata acctgaatct tctaataaag atataaagaa aaagagcaca acaatttgtg
    14881 gtacaaagac taatactgat cctacaccag caataatgcc atctgtaatt aaatcttgta
    14941 aaaatggtat aacgccaaga taattcataa tcgtcttcac actatccgta aatgtaccac
    15001 ctataaatgc atcgagttga tccgacaaag gtgtgccaat ccatgtaaat gtagtttgaa
    15061 agatcaacca cataatcgct agaaagatag gcatccctat atatttatgt gttaatatct
    15121 tgtctattct agagctgaaa tattgcttat cttcatctgg atacgttacc acgtcttgca
    15181 ataacgtctc aatataatga ttgcgtatac gctccatctc tcgacgaaca gatacagctt
    15241 ctacttgttt agcaacttga tcacgtaaac tcgacaattt atttacaacc tctgaattaa
    15301 gttcgtttgc aatttgcatg ttatttaata agaattgaat cgcaataaat ctagcttgat
    15361 atttatcatg agatgtctct gtcattatta tttgacacat atttttaatt gtctcttcaa
    15421 tcttctcacc ataattgatt ttaaaatgcg gttgataccc ttcccctaga tgctttattt
    15481 cgccaagtaa atgtttagtt cctttgcctg tacgtgccac aactggaaaa ataggtgttt
    15541 ttaacttttt catcaattta tgataatcga tttttatccc gcgctttgtt gctacatcaa
    15601 tcatatttaa tccgatgtat attggttgat taagttctaa caattgtact gttaattgca
    15661 tatttctttt tagttgactc gcatcaacaa tgttaatgat tcctgaaaat gaatcgttta
    15721 ataaatagtc tgtcactaca gtttcatctt tagaaatcgg cgataaatca tatgtacctg
    15781 gtaaatcaat taattgtcct acattttctt taagctttcc tacttttttc tctaccgtta
    15841 cgccactcca gttgcctata tattcatacg aaccagttaa agcgttaaac aaagatgttt
    15901 taccaacatt aggatttcct aaaatacaat aattttccat tcgtccggct cctattcttc
    15961 taatgcaata gaacaagcat cgcaatgtct aatacttaac tgttgtccgt ttacttcaat
    16021 aatacatggc cctttaaata aacatttttg tttaatcgtt atgatagcgt catctgttaa
    16081 cccaaaggca cttagacgat acaacatatt ctcattagca atatccattc gctttatttt
    16141 ataagcctta ttcatttcac catttttaat gtttaacata ctatttcgct ctcctattag
    16201 aaataataat cattatcact taaaaatcat aacccttaaa attgtagctc gcaatacttt
    16261 atttaaataa ttttcatttt tcatataaaa tttgtgacat tacaaaaatg ttttatataa
    16321 tctcttacat gcaaaaaacc tccacttcaa atatcattga tagacattta aagcggaggc
    16381 atgtacatgt tattttatga tttatttctt aagttttttt ctaacaattg attgagcttt
    16441 tcaatatttt tattcaactg ttctatgttc gttacttgtt gttttgaatt tagttgatgc
    16501 ttgttttgat aatcaattaa agcatcatat aagaacaatg aacttgctga ttgctgtttc
    16561 acttcttggt acaatgccat cgttttatca tctagtacta ttgaatgtgc atcatcttgc
    16621 ccatgtacat cttgatattt cgatacattt gttgcacctt gtggatatac tttaaaggtt
    16681 gtgtcgtatg tttccttact atctttagct tcagattcct gtgattcaac cgttttatat
    16741 ttttcaagtg catgtccttc aatatcaact cgtggaataa tgcgattcaa ccatgctggt
    16801 aaataccacg aaccttttcc aaacaattta gttaatgcag gaattaacat cattctgact
    16861 acgaaggcat caaagagtac accaaacgct aatgccatac ccattgattt aatcatgaca
    16921 tcttcttgga atacaaacgc aaagaagaca ctaaacatga ttaatgcagc tgctacaata
    16981 acaggaccac tttctttcaa tcctactttg atagaataat cattatcccc tgttttacta
    17041 tattcttcat gaattcgcga cataaggaag acttcgtaat ccattgctaa gccgaatagt
    17101 ataccaattg taataaccgg caagaaggct agaattggtc ctgtcgtttc tatgccgaat
    17161 aatcctttca taaaaccatc ttgcattact aatgttgtaa atcctagcgt tgccattaat
    17221 gacaagacaa atcctaaaac tgcttttaac ggtattagaa ttgaacggaa gacaatcatt
    17281 aataagaaaa atgccaatac aacaatgact gttgcaaata aaggtatcgc ctcatttaac
    17341 tttttagaca tatcgatatt aatgacactt tgtcccgaaa tctccgtttt gaacccatat
    17401 ttatcttgtg catctctatg ataatctcgt aaatcatgca ctaaatcatt tgtgctctct
    17461 gcattaggcc cttgcttagg tatcacgacc atcaaagcat aatcattatc tttactcatt
    17521 tgtggcggcg taacgatatc tacatttttc ttatctttaa tatctttata cacagattgt
    17581 aaatcttgtt gtaatccttg tggatcatcc tttttatctt tcacatttat caacatcgga
    17641 atttggccat taaatccttc accaaattta tccgagataa tatcgtaagc ttttttctgt
    17701 gtagaatctg ctggtttaac accgtcatct ggaataccaa gtcgcatatg actaactggt
    17761 attgcagctg ctactaatat gattaaacct agtaatactg ccgcaagtgc atttcctgta
    17821 ataaatttag accatggcgt atcaatatct tttttgaatt tagactgtaa tttattcact
    17881 ttaatgcgtt tatggaaaat gcttattaat gcaggtaata aagttaaagc gctaagtact
    17941 gcaaaaacaa cactaattgc cgaagcaaat cccattaccg ctaagaagtc aatacctact
    18001 aatgataaac cacatactgc aatcacaact gtcacaccag cgaagatgac tgcgctacct
    18061 gctgttccca ctgcaagtcc gataccttta acatagtctg tttcagtttt cattacttgt
    18121 cgatatctaa acaaaataaa taatgcataa tcgataccaa cagcgagccc aatcattacc
    18181 gctaatgtca gtgtgacatt tggtatatca aatgcataag ttaacaagct gataatgcca
    18241 accccagatg ctaaaccaat caatgcactt ataattggta atcctgcagc aatgactgaa
    18301 ccaaatgtaa tcaataacac tacaaatgca acaatgatac caactagttc agaattaccg
    18361 cctacttctg tagatgtcat tcctgtacca gttaactctg tttgtacatt atgattgtca
    18421 cgtaagtctt tcaaatgact tttaacatta tctctagagc catcttttaa agatgtttga
    18481 ctaacgtcat atgtgatatc tgcaaatgct gttgttttat ctttactaat ttgcttattt
    18541 tcataaggat ctgatatttt atcaatgtgc ttgtcatctt ttttaatatc atctaacgtt
    18601 ttcttaatat ctttagtaat gttcggttgc acaataccat catctttagt cgtcttaaag
    18661 acaacacgta tttgtgcctt ttcactatct tgattaaaat gtttttcaat ctttttattc
    18721 gtatctaacg actctaatcc tgtcatttta atatcattgt caaatttcgg tgcatttgta
    18781 gcaagtggta tcaatattgc agctacaatc actatccatg caatgaccgc ggaccattta
    18841 tgttttgcga tgaatgtccc catcttatat aaaaattttg ccaaagtata ttgcctcctt
    18901 ttaaaatcaa cgttatagtt taactataca gtgtagatta ttgtttgatt atagtatcta
    18961 tccccgacct cttaaagaat caattggaaa attttgtata ttaaactaca cacaaaggag
    19021 aaatgtagat gaaagagact gatttacgag ttataaagac aaaaaaagca ttgtcaagta
    19081 gcttgctgca attgttagaa cagcaattat tccaaacgat tactgtcaat caaatttgcg
    19141 acaacgcact cgtacaccgc acaacatttt ataaacattt ttatgataaa tatgatcttc
    19201 ttgaatattt atttaatcaa ttgactaaag actatttcgc cagagacatt agcgatcgtc
    19261 ttaaccaccc atttcaaacg attagcgata caattaacaa taaagaagat ttgcgagata
    19321 ttgcagaatt tcaagaagaa gacgctgaat ttaataaagt attaaaaaat gtctgcatta
    19381 aaattatgca taacgatatc aaaaataata gagaccgtat cgatattgac agccacatcc
    19441 cagataatct catattttat atttatgact cgttgattga aggttttatg cattggataa
    19501 aagatgaaaa aattgactgg cctggtgaag aaattgataa aattttccat aagataataa
    19561 atattaagat taaatagtag atgagaggct cataagcgtc accagcattc ataataaaaa
    19621 cgatagttta cacattaatg cattcgtgta ctactatcgt tttttatttt tatcgtgctt
    19681 atcgctatta aaataactga tacacaacac ataaactatg aagaaaaaaa taaatccgct
    19741 atctaaatga ctttgactca gttgtttcaa tgaccaaatt gctaatacaa ttgccatgat
    19801 tattgaaata acgtatctca tattcttata cctatcatcc ttttctaaaa atatggttgc
    19861 tattacttaa tttttaaagt tataaataaa aagagccaac cgcaatggat ggcccttgtt
    19921 cattatgaag cattagaaca tttctgaaac aaccttttgt tctaagaagt gtaataagta
    19981 gtctgggcta cctgttttag cgtcagtacc tgacattttg aaaccaccaa atggatggta
    20041 tccaacaact gctgaagtac agcctctgtt aaggtataaa ttacctacat caaattcgtt
    20101 taccgcttta atccaatgct cgcgattatt tgtaatcact gcaccagtta aaccgtaatc
    20161 tgtatcattt gcaacctcaa ttgcttcatc aaaatcgtta actttcacaa agccaacaac
    20221 tggaccaaaa atttcttctt gcatgattct atctttagat ttaagtcctg aaatgattgt
    20281 tggttctaca aagtaacctt ttgaatcatc agtgccgcca ccttgttcta atttaccttc
    20341 ttctttacca atctcaatat aatttttaat cttatcaaat tgttttttat taataactgg
    20401 gcccatatac gtattgtcta cagtattgcc caacgttaat tcttttgtta atttgattga
    20461 tttctctaat acttcgtcat aaacgtcttt atgcacaatt gcacgtgaac atgcagaaca
    20521 tttttgacca gagaaaccaa acgctgacgt tacaatagct tctgccgcca tatctgtatc
    20581 aatattttca tcaactacaa tggcatcttt accacccatt tcagcgataa cacgtttcaa
    20641 gaagttttga ccttcttgaa ctacggcact acgttcataa attctagtac ctgtcgcacg
    20701 tgatcctgta aatgtaacga aatgcgtatc tttatgatca actaagtaat caccaatttc
    20761 tttcggatca ccaggaacaa agttaactac gccttttggt aatcctgctt cttctaaaat
    20821 ttccattaat ttataagcga tataaggtgt atcctcagca ggtttcaata acactgtatt
    20881 acctgccaca actggtgcta aagttgtacc agccataatc gcaaacggga agttccacgg
    20941 cggaattgta acacctgtac caattgattt atagaaatat ttattgtgtt caccttcacg
    21001 atcaagtact ggcttgcctt gagccaagtc catcattgaa cgtgcatagt attcaataaa
    21061 atcaatacct tcagctgcat caccaactgc ttcatcccat ggcttacctg cttcataaac
    21121 cataattgct gcaatttccg cttttcgacg acgaataatt gccgaaacac gtaacataag
    21181 ctctgcacga tcatttgctg accatgtttt ccaagattta taagcttcgt ttgctgcttt
    21241 aaatgcatct tcaacatctt gttttgttgc ctttgatgca tttgcaatta cttgtgatgt
    21301 gtctgcagga ttgattgatt taattttgtc atctttgaaa atcttctctc cattaatcac
    21361 taatggtatg tcttgaccta attctttttc cacgtctttc aatgctttct taaacatatc
    21421 cacattttct tggactgaaa aaccgtaacc aggttcattt ttaaattcta ctaccatgta
    21481 cacttacccc ctataaattt tgaaagtggt ttaacccttt gatttaatga tataacatca
    21541 tttaaactca ttttactatg attaaggtca gttttgcaat cgctttcatt tttatgcttt
    21601 atccctcatt ctcaagcatt ttgaaattga ttggttactt tttaaaattt atatgtgtcg
    21661 caactactac tttatcgttt cgtcatttaa tgtttcggat ggtaggtcat tatcaatttt
    21721 acgaacgact ttacaagggt ttccaaccgc taagctgtgt ggcggaatat ctttagtgac
    21781 aacactacca gcaccaatca cactaccttc tccaatcgtc acccctggta gcacggctac
    21841 atgaccgcca aaccaagtat tactgccaat atgaatgggt ccggcttttt caaaaccttc
    21901 atttctatga tgaaaattaa gtggatgtgt cgctgtgtag aatccacaat taggtccaat
    21961 aaaaacatta tcgccaattg taatctgtcc accatccata aaataacaat tggtgtttac
    22021 atagacattt tttcctagtt taacgttcca accataatct gtatcaaaag gaatcgaaat
    22081 acttacatta tctgttgttg tttgaaataa ttgatcgatt aattcctttc ttttaattgt
    22141 gtcactcggt cttgtatgat ttaattcaaa gcaaatatct ttcgctcgtg cacgttcatt
    22201 gattaagtct tgatcaaagt ttgcatcgta ccatttttct gctaacattt tttctttttc
    22261 agtcattaca cctttcaact cctaataact tatttacttg tttaaaagtt aatcaaataa
    22321 acattcgcct atgcaactaa tacgctataa cattatgaaa tcgtgacctt atcaccctta
    22381 tctatacaat tctcgcatca aatactgcta aagtagtaga taaattcaat actacagacg
    22441 cattcatttt ttaatctatt aacgtacaat gtgagtaaga gaaatataaa ggagtatgat
    22501 agcgatgaga atattaatta caggcacagt tgctatctta atcattctag gtttagttaa
    22561 aacgatacaa gattacgaaa tgacaaacga cacaagtcgt cagatgtcag acaacaaaga
    22621 tgatgataaa gtcatccatc ttaataattt taaaaattta catgcgaaag aatttaaccc
    22681 atctgatttc ttttaagtca cctaagaatt gcaaatccag aagtcattta agttttacct
    22741 ttcattcata catcctttaa tattaattac gacttctttt atatagatgc taaatagaga
    22801 gattgttgtg caatgtttgc acggcaatct ctctttttct ttttaaaatt ggtaaaagta
    22861 aaacgcaacg attgacttat atacctatag ggggtacatt agacgtgtaa caatgaatca
    22921 cagggaggca ataatgtggc taatacgaaa aaaacaacat tagatatcac tggtatgact
    22981 tgtgccgcat gttcaaatcg tatcgaaaag aaactgaata aacttgatga cgttaatgcc
    23041 caagtgaatt taactacaga gaaagcaact gttgagtata accctgatca acatgatgtc
    23101 caagaattta ttaatacgat tcaacattta ggttacggtg tcgctgtaga aactgtcgaa
    23161 ttagacatta caggtatgac ttgtgctgca tgctcaagcc gtattgaaaa agtgttaaat
    23221 aaaatggacg gtgttcaaaa tgcaactgtc aatttaacaa cagagcaagc taaagttgac
    23281 tattatcctg aagaaacaga tgctgataaa cttgtcactc gcattcaaaa attaggttat
    23341 gacgcgtcta ttaaagataa caataaagat caaacgtcac gcaaagctga agcgctacaa
    23401 cataaattga ttaagcttat catatcagca atattatctt taccactatt aatgttaatg
    23461 tttgtgcatc ttttcaatat gcatatacca gcactattta cgaatccatg gttccaattt
    23521 attttagcta cacctgtaca atttattatt ggatggcaat tttatgtagg tgcttataaa
    23581 aacttaagaa atggtggcgc caatatggat gtacttgttg ctgttggtac aagtgcagca
    23641 tatttttaca gtatttatga aatggttcgt tggctaaatg gctcaacaac gcaaccgcat
    23701 ttatactttg aaacaagcgc cgtactaatt accttaatct tattcggtaa gtatttagaa
    23761 gctagagcga agtctcaaac aaccaatgcg cttggcgaat tattaagttt gcaagctaaa
    23821 gaagcacgca ttttaaaaga tggtaatgaa gtgatgattc ctctaaatga agtacatgtt
    23881 ggagatacac ttatcgttaa accaggtgaa aagatacctg ttgatggtaa aattattaaa
    23941 ggtatgactg ccatcgacga atctatgtta acaggtgaat ctatacctgt tgagaagaat
    24001 gttgatgata ctgtaattgg ttcaacgatg aacaaaaacg gtactattac tatgacagca
    24061 acaaaagttg gcggggacac tgcgttggca aatattatta aagttgtcga agaagctcaa
    24121 agttctaaag cgccgattca acgattggca gatattattt ctggttattt cgttcctatc
    24181 gttgttggta tcgcactatt aacatttatc gtgtggatta ctttagttac accaggtaca
    24241 tttgaacctg cacttgttgc gagtatttcc gttctcgtca ttgcttgccc atgcgcatta
    24301 ggacttgcta caccaacttc tattatggta ggtactggtc gcgctgctga aaatggtatt
    24361 ttatttaaag gtggcgagtt tgttgaacgc acacatctaa ttgataccat cgttttagat
    24421 aagacgggta ccattacaaa tggtcgtcca gtcgtgacag attatcatgg tgacaatcaa
    24481 acgctacaac tactcgctac tgctgaaaaa gattctgaac acccattggc agaagccatt
    24541 gtcaattatg caaaagaaaa gcaattaaca ttaactgaga caacaacatt taaagcagta
    24601 cctggccatg gtattgaagc aacgattgat catcaccata tattggttgg taaccgtaaa
    24661 ttaatggctg acaatgatat tagcttgcct aagcatattt ctgatgattt aacacattat
    24721 gaacgagatg gcaaaacagc tatgctcatt gctgttaatt attcattaac tggtatcatc
    24781 gcagtggcag atactgtcaa agatcatgcc aaagatgcta taaaacaatt gcatgatatg
    24841 ggcattgaag ttgccatgtt aactggcgat aataaaaaca ctgctcaagc cattgcaaaa
    24901 caagtaggca tagatactgt tattgcagat attttaccag aagaaaaagc tgcacaaatt
    24961 gcgaaactac agcaacaagg taagaaggtt gcaatggttg gtgacggtgt aaatgatgca
    25021 cctgcattag ttaaagctga tatcggtatc gccattggta caggtacaga agttgccatt
    25081 gaagcggctg atattactat tcttggtggc gacttgatgc ttattcctaa agccatttat
    25141 gcaagtaaag caaccattcg taatattcgt caaaatctat tttgggcatt cggctataat
    25201 attgccggta tccctatagc tgcattgggc ttacttgcgc catgggttgc tggtgctgca
    25261 atggcactaa gttcagtaag tgttgtcaca aacgcactta gattgaaaaa gatgcgatta
    25321 gaaccacgcc gtaaagatgc ctagattcct taataatgaa ggattcgttg gtgattctga
    25381 ggtagactag tgattggctc tataatgtcg cggtttacag ttggatcttc gctcctactg
    25441 catatatagt tacacttttc gcttggcgaa ttagtgtgtc ttacctaata gctccgccta
    25501 ttaggttcca acattattat aaataataag tacactatgg tttacagttg gatcttcgct
    25561 ccaactgcat aagagccact aattattata aataataagt ggctctaaaa ggaggtgaat
    25621 gctatgtcac aagaaatttt aaatgttgaa ggtatgagct gtggtcactg caaaagtgct
    25681 gttgaatctg cattaaataa tattgacggt gtcacttcag ctgacgttaa ccttgaaaat
    25741 ggtcaagtaa gtgttcaata tgatgacagt aaagttgctg tatctcaaat gaaagacgca
    25801 attgaagatc aaggttacga tgtcgtttaa ttaggcaata ttcaacgtca tcaacaccaa
    25861 attaaaaaat cgaactgatg agaatcccaa caatccaaat tatctcatca gttcgatttt
    25921 taatttactc gcaacctggt atctccagtc tgcaatacat ctaatgttgc atctaatgca
    25981 tcgacaatta gattttcaac tgcagcttca gtataaaacg caatatgtgg tgttaatatg
    26041 acatcttccc tgtcaatcaa cgattctaac aatggatcgt tcagtgtttt gcccctttga
    26101 tcacttggga aaagtttgcg ttcaaattca tacgtatcaa gtgctgcacc tttaatcaca
    26161 ccattgtcta atgcttctaa taacgcctta gtatctacta aagaacctct cgcacaattg
    26221 acaaatactg cgcccttttt aaaatgttta aataattcag cattaaatag ataatgatta
    26281 tatttcgttg caggtacatg taatgtcacg atatcagcac cttcaaccgc ttcctcaatc
    26341 gtatctttgt aatcgacata cgttgcaatt ttagcattag gaaacgggtc gtatgcgacc
    26401 acatcacttt gataaccatt ggcaaatata tcggctacta cacggccaat tcgacctgta
    26461 ccaataacag ctacttttaa atctttaatg gatttcgata aaatagtagg ttcccatcta
    26521 aaatcatgct ctcgcacttt cgtttgaatt tgattaaaat gacgaaccac attaatagcc
    26581 tggttcacag caaactccgc aattgaattc ggagagtatg acggcacatt tgacacaatc
    26641 aagttatact tgtttgctaa ctccaaatca tatgtatcaa atccagcact tcgttgtgcg
    26701 atttgtttaa tacctagttc atttaatcgt ttataaacat gctctgataa tggtatttgt
    26761 tgtgatagcg ataagccatc ataaccagcg acaccttcaa cattgtcatc agttaatgct
    26821 tctttagtaa tatctacctc aacatgatgt ttctctgccc acgccttgat ataaggcata
    26881 tcttcatcac gtacactcat gattttaatt tttgtcattt taacatcacc cttaacttta
    26941 ttattcatat aaatatgcta gttctgttaa tcttattgca gcttcgtcta atttctggtc
    27001 atctaacgcc aatgaaattc tcacataacg attaccattc tctccaaatg gtttccctgg
    27061 agcaacaagt attgacttct ctttcactaa aaattgctca aattgctcgc tgtcataacc
    27121 aggcggtgtt tccaaccata catatatgcc acctttagca tgaacaaatg gcaaatcagc
    27181 ttttgcaagc atggcttcga atcggtcacg acgtgcttta aatatattgc tttgttcttc
    27241 taaaaaatca tcataatgat tcaaagcata tattgcggca tcttgtaatg caccaaacat
    27301 cccagcattt gtgtgcgttt ggtacttttt caaagcttga atcatatctt tattaccaac
    27361 tgcaaaaccg actctaaaac ctgacatgtt ataaccttta gacaaggaat aaatttcaat
    27421 cgcaacatct ttcccatttt ccgaagcaag tatactagga tttttagcgt cgaaaccgaa
    27481 agcaccataa gcaaaatcat gtacgatttt agtgtctgta tctttaaatt tagctatcgc
    27541 ttcatcaaaa acttctttcg tagctgtcga tccagttgga ttatttggat acgttaaata
    27601 aatgagtttt gttttatcta ttatttgtga atcaactttg gaccaatctg gcaaataatg
    27661 tggcggttct aaattaagtg ggacaggctt gccatcagct aaaagtacac ctgctaaata
    27721 atccgtgtag cctggatcag gtagtaatac atagtctcct ggattgataa cacatgttgg
    27781 tactgccact aatccatttt ttgtaccata taaaatgcat acttcatctt ctttatctaa
    27841 cgtcacatta tattgtcttt gataaaaatc tacaatagct tgcttgaacg cttctttacc
    27901 atgaaaagca ccatattttt gattttcagg aatagttagt gcttgttgaa aatgatcaat
    27961 aataccttgt ggtgtgggcc catcagggat cccaactgcc atattaatta atggcaatgg
    28021 tccatgttcg attttacgtc ccatcgtttt cccgaaataa ctatcaggga tatttgctaa
    28081 tttgttagag atcatcaaat tcctcctcta tcattaaaca tagcctgggc gactatcata
    28141 atcctaacaa cttgtatcgc tctcatttag atggttacaa tgacatcgcc attcaccgtt
    28201 atgttcaaca gaacttatga cacacgttgt attgaatgaa tttattttca ttttaggtag
    28261 gtacattatt attgtcaata ttaggaattt tcagatcaat atacgctcaa tcgttatgat
    28321 ttaactgtca tgcatatccg cttgcgcaac cagtaagata tgcttatata aagtataacg
    28381 cccatcaagg tacgtattca aacgtgaacc ttaacaggcg tcattcattg ttaaataaaa
    28441 cttctttagc acatacttat ttcactatgc cttttacgtt ccccttatac ttttctcaca
    28501 tctttctctt agactactcc cttacacgcc acgctcaata tctttaatca tttcgtctac
    28561 agttattttc gcactcgtta agacaatagg aacgcctgca cctggatgcg tacttgcacc
    28621 tgcaaaatat aaatctttat aatctctcga tacattttgt ggacgataat aattactttg
    28681 cgctaaagtt ggcattaaac cgaatgccga accaaatttc gcatgatacg tttgctcaaa
    28741 atcatttggc gtaaagattg tttctgaaac aatatgcgat tttatatctt caaatacttc
    28801 aatcgctgct aatttacgat aaataatttc ctttatttgt tgcgtcaaag cttcatctga
    28861 ccaatcgatt ccgctacctg ttttaagttc cggcgtcggc attaacacat aaataccagt
    28921 tttgccttct ggcgcaagtg atttatcagc gaccgctgga acatacacat aaatagaagg
    28981 atcatatgat aaacgtccct caaatatttc ttcaatattg cctctaaaat catctgaaaa
    29041 aataacatta tgaagtctca cttgatctgt cacatcaata tctataccga tatacattaa
    29101 aaatgctgaa caagagtaat ctaagtctgc aattttatgt ggtggatact ttttaatagg
    29161 tgcaaaatct ggcattaatg attccgcaac actagggaaa tcagccgtac ataaaatttt
    29221 atcaaatttt cttatgtcac cattcacttt tatcgcatca gctcgtttga atttaggatc
    29281 aataataatt tgctcaattt cagcattaag ttcaatatta acacctaaat ctttatttaa
    29341 ttgcgctagc ccttgagcca tgccatacat accgccttta ataaaatgca caccaaacat
    29401 catttcaatc ataggaataa ttgaatatag tgacgggcct cgttttggat caattcctat
    29461 gtataacgtt tgaaacgcta aaagcttttg tatcttttcg ttatcaatat aatgttcaat
    29521 tagctgatct gcatgattta acgtttttaa cttagcacct tgcacaagtg acgtcatatt
    29581 ataaaagtca cttggtttgc gatacgttct ttctaagaaa tagcgacgtg caatttcata
    29641 ttttttataa acatccgtta aaaatgacat aaaaccatgc gttgaaccag gttctatact
    29701 ttctagcatg tgctgtaatt cagctaaatc tgtaggcacc gttatacgat catcgcggtc
    29761 aaaatacaca tcgtaaatat aacgtaattg tctcaattca atataatctt cataattttt
    29821 accacacatt gtaaatacat ctttataaac atctggcatc atgacaattg tggggcccat
    29881 atcaaatgta aagccgtctt tctttaattg attcatacgc ccgcctacgt tagtattttt
    29941 ttcaaatatc gtcacttcat gaccttgaga agcaatacgg gctgccgctg ctaatcctgt
    30001 gacacctgca ccaattactg caatcttcat tattcaacca cctatattct atgatattta
    30061 ctatttattt catgaaacaa ctttgctttt ttccgcttat ccacaaaaac acgttcatgt
    30121 aatgtatagt tagcctgtct cacttcgtcc agtatttcaa tatatatacg tgctgctaat
    30181 tctatgattg gttgtgcttc aatactaaat actttgattt gatccataac atctcgaaaa
    30241 tctttttctg cgatagctgc ataatattcc cataagtcaa tataatgatt attaacacca
    30301 ttttggtaca cttcagcaat atcaacttca tattgcttta atcgttgctt actaaaatat
    30361 atccgttcat tttcaaaatc ttcaccgaca tctcttaata tattaatcaa ttgcaacgat
    30421 tcaccaagcc ttcttgcgac atcgtatgtc tgatgtgttt catgatcact taaaatcggc
    30481 gtcaatactt cacctactgt accagcaaca ccataacaat atccgaataa ttcagcgtcc
    30541 gtttcaaaca ttgtaaattg ttgatcttta tatacagtat caatgagatt atagaaagat
    30601 tgaaaggcga tatttttatg ttgtgcaaca tgctgaagag ccatcatgat tctacgatca
    30661 ctttgaaagt gatgatgttc atatgggtat ttttcaatag attgtatatc ttcttttatt
    30721 tgatttaaaa attgaatatc gccataaaca tctatactgt catcaatttt acgacacaca
    30781 gcataaattg cccaaaccgc ttttctttga tcttctggta acaagtcaaa agcgtaagaa
    30841 aagctttttg aatgtttctt catgatttta tgacaatatt taaaattcat agccatcatt
    30901 gtcatactag tcctcctata ttgaaatgcc attaccttga tttattgttc tttagatata
    30961 gaatattgac gacctttcca ttcaactaca ccataacgat gcgtttgttt ccaagattgg
    31021 atgaaaattt tagtgaaaaa cataaataac aatggatgac ataccattaa taaattagaa
    31081 aattggccga cccttcgatg cagatgaata aattgtgtag tatagctcaa gtaaactgtt
    31141 aacatttttc ctacagacat ttggcgatat tttaaactaa gacatagccc taaaatactc
    31201 gcgatagaac cgaacaacca caacacaatt gctgccatga tcttaggctt tgtgccatct
    31261 gccccagtcg acaaatgctt tgtccatcct tcttgtaatg actgaaatcc ttcttgatac
    31321 atgcgaaatg caacaaatgg aaatccttca taaactgtta cgggcaatga ttgtgaagta
    31381 tatgcacttc ctaaagcaaa tccttcaata atatgacggt tcgcactttt atgacctcca
    31441 gttgcataat aatcttcttt atttgttaat gtcaccggtc caaatgcatg ctggttagtc
    31501 cgaccgtcgt ctaaggtaga aaatacattc ataccaacga ctgtcattaa attaaatatc
    31561 gctgaaaacc cttcgtagaa acgctttgtt atgtgataag gctgtacgct taacaatcct
    31621 ttttcatctt gtaattgata ctgattaatc aacgtttcaa cagcatcttt ccttaagaaa
    31681 gttacatcag catctacaaa agcaatacga ttcgtacatg catgcgtcac accttgataa
    31741 caagcatgtg atttcccata ccatttgccg tcagcatcat caacaacatc tactacagtc
    31801 gcaccatatg aacgcgctac acgtgctgtt tcatctgtcg atccgtcatt cataacaatg
    31861 acatctactg gaacttgctg ttgtattatc gaatgtagta aatgacctat tcttttttct
    31921 tcgtttctag ccggaataat aattgttaat gatttatgat tgaagttcag cgttttcgcc
    31981 tttagctgat gtctacgatt aaatatcaat gcaccacacg ccatagacat ggtcactatt
    32041 actgttaata ttcgtgataa ccatttcata gatatcactt cctattcttc gcttctcgcg
    32101 cgtttatttt gtctgcgact tgttgcccac ttaatgtaac cattggcatg ccaccaccag
    32161 gatttactga tccacctaca aagtacaagt tttcaaaata ctgactttct ttaggaaatt
    32221 taaatccttt gtttttcttt ttatctgcta caacaccata tattgcacca cgattagaac
    32281 gataattttt ttcaatatcc tccggtgtcc aaacatcttc ataaataatg tgttttctta
    32341 aatcagtaag tcccattttt tctaatttat ccaaaatttt atccctaaac ttcgcataat
    32401 cttcagtggt aaaaggctga tcttgaatat atggaatatg tggtaagact ttgatatttt
    32461 cataacctac tggcgcttgt gtgtgatcag ttttattcgt atttactaga taaatggtcg
    32521 gatcatctgg caataccttt tcatgaaaaa cttgttgata attgagataa gcattttccg
    32581 taaaaaagaa attatgatgt gctaattgcg ggtattggca agcaacacct aaatgcatca
    32641 catatcctga gcttgccggc tcaaattccc tctctaattt gtttaatcgt tgagtatcaa
    32701 ggtgaattaa atatttataa gtaggtatga cttccatatt tgaaataata taatctgcct
    32761 ttacaaactc acctgtatct aatctgacac ccgtcacacg tctttgatat gttttaatat
    32821 tgtccacacg tgcacctgta tgaattgtga caccttcttc acgcgctagc ttttccaagg
    32881 cattggctaa atgatggatt ccaccttcta cataccaaag gccttgctct tgttgcatat
    32941 ggaataacat agataataca gctggcgcat cgtatgatga agaacctaca tatttgataa
    33001 aatagcctaa catttgtcgc aagtatggat tcgagatacg cttgtctatg gcctgttgca
    33061 tagtatgtac ataatcataa ttaataagag catttaatgg cccatgaaat ttaataattt
    33121 gagatagtgt atctaaacca tagttaaaat accctttttc agtaatacga tcgattcgtc
    33181 ttgtataatt caaataattt tgcagttcct ctatatcctg tttcgacaat atcacattat
    33241 gctgacctgt ttctttaata ccttcataca aatcgatagt cgttccatct ggaaaaaagc
    33301 tacgccattg atgtggcaat cgcttgattg taacgtagtc tgacatttgc ttattgctat
    33361 attcgaatat tttttcaaaa atataaggca tcgttaaaat agatggacct aaatcaaagc
    33421 caaagccatc tgattcatga cgattcactt tgcctcctat atgattattt tgttcatata
    33481 atgagaccga atagccactt tgcgccattc gaattgctgc agaaatccca cctaagccac
    33541 caccaataac gatgatatgt ttagtcatga cgttcacctc ttcgttttaa tatgcgtagt
    33601 aactgcgtta atctcggtcg attataacgt tgcacaataa tgattggaac attagttatc
    33661 attgcataga aaatatttat atactttact aaacgagggc ctttattaaa tatgatgact
    33721 ggaagtatcg atatccaatg gatcaactct gtcctctttg tctctataat cattttttca
    33781 atttcatcag tattgattgt cattaaatga cgctgatcat atatattttg attaagctga
    33841 tgaccatcta aaatcttatg tttccatttt cttacgtaaa accatttgtt ccaaaatttt
    33901 ccatgcttct caaagttaaa tgatttaaat attatatgct tctgacgaaa atacttgtca
    33961 ggaattcttg tacctaaatt tgctatattt agttgaacaa ttaaccaata catactacaa
    34021 aaaaatgctg ttttgatata ttttttcatg gttttcatct aaattgaatc actctcaatc
    34081 atactgacat agtataaatt atcattttat tacttagttc cctaaagatt agtgaataaa
    34141 ccgctctcat ttatatttat ataaaacaaa aacgccaata ctctcatgtt agagaatatt
    34201 gacgtcattt gatttacatt tgattaatga atgaaattat atgaacctgc ttggtttgct
    34261 gaaattgtac gagacgtaac cacaccagca ccatgtccat agttcatttc tgaaacacga
    34321 acagaaccgt tgctgttaac gccttcaacg taagcaacat gaccgtaata gccttgtgtt
    34381 gtttgcatga tagcaccagc ttttggtgta ttgttcactg tatagccaga tgatgcagct
    34441 gcgctagccc aattacttgc gttaccccat gttgaaccaa ttttcccacc aacacgatca
    34501 aatacataat aagtacattg tcctgaagta tataagttac ttcctgatgc ataaccatta
    34561 gaaattgaac gaccatttga agatggcgct gcagttgtag tcacatgaac attattactt
    34621 gttgtgctat aacttgcacc tgaaccacca gtataatagt tatttgttgc tgtttggcta
    34681 ttgtttgtat agttattgta ttgatatgaa tgattatagt agttattgta agatgcattg
    34741 ttataactat acgtattgta gttattgtaa tagtatgtgt tgttttgcgt taattgactt
    34801 ggattccaat ttcctgtcca agtgtaatga taattacctt gtgcatcaat tgtatacgta
    34861 tagctgtatg attgagcgtc attagaatta tatccattgt tattttgttc tgcggcttgt
    34921 gcatcatgtc ctgcaaatgc gatagtggca agtcctgctg tagcgattgt agctgtaacg
    34981 atttttttca taattaaata tcctcccaat aatcaaaaca ctctatacat atagtcatta
    35041 gcgactcaat tattttaaat tttatatttt ttattttttt agtatgcaaa taattataac
    35101 ttttatagtt ttgcgacaaa ataaactata acagagatta ggtgtcttgc gggctttgtt
    35161 atcataatgt aatctgttcc taataaagct tcattgaatg taatatacca attagaatgt
    35221 ttacaacact ttttctgcac cgttaacatg ggatttgaaa ataaaaaagc actaaacttt
    35281 taatgaaata cacacgaact gacaataaat atcagaagta atgtaatatt tcaaaagttt
    35341 agtgcatcaa gttatttttt atttgtagca tgtgtttcca tcgtttttac aatcaaatca
    35401 gtcagcgctt tactacctgc atattccaaa tgtatgccgt catatgcaaa gtattccgga
    35461 tgacctgcag aagctttata ccaatcgact agatgtacat tagagcgctt ttcagctgcc
    35521 tcataaatta atttatttat tctaccttca taatctctag gtactctaat agaaactaaa
    35581 taaatgtctg cttttccaaa actatccaat agttcattta attgatcttt cgtaaatgcc
    35641 ccatttgtac caagctctac tacaactttt tgaccttttt tagcatagtc tttatattgc
    35701 gatttcacaa ttggtgtagc atcaacgagt tgccgtccaa ctttaccatc aatttgtgca
    35761 tttggtattt tcttagtaaa gacattacca atatccacca tgaccgagtc accaattaat
    35821 agtggtgatg actttttaat atcctctttg ctattcgctg tctgcttatc ctcttgctta
    35881 tcttttttaa cgactttttt cttcgttgtt ttaaattccg tcttcttctc tttcttctcg
    35941 gcttcatgtt gtttgccaag tgcatcaaac tgtccactga gcacaacgat agacggaacc
    36001 aataatagga taactaacac tgttctagca aattgcccct tctttttagg taaaaatgca
    36061 aaagctttaa atcctttttt acgtataggt gtttcaataa agcgatacga aatttcagcc
    36121 attaacgctg ttaatacaat ttctatcaca tagacgtata caggtatttg tccttgtacg
    36181 taataactgt tcacaaaaac aatgatagga taatgccata aatataagct atatgatcgt
    36241 ttacctataa ttagcaaagg tttcatactt aaaaatttag caaataaact agaaggatgt
    36301 accgcaattg caataatgaa taaagttgca aatgatataa tgtaaaatcc tccattatag
    36361 atccattgat cttggtctcc aactataaag aacaaagtca ttagaaccgc aaaaccagat
    36421 atccctataa tatctaatga tacgacaatc tttttagaaa tatctttttt caaagcaaac
    36481 ggaggccaaa taaatgctaa tatacaacca agcaataaag tttgcagtcg tgtatctgtc
    36541 ccaaaataca cacgtgaatt atctccagtg atgaaatgaa tcactatcat aagtcctaaa
    36601 gaaatcaacg atacaataaa tagcgtttga ataatatttc tcggtttaaa tctatgtaat
    36661 aagaacgtga taaccaatgg gaaaagcaag taaaattgtt cttcaatggc taaagaccat
    36721 aaatgtttga gtggttcaat agcaaattgg ttaaaataat ctacattctg tgaaatgtac
    36781 caccagtttg aaacatagaa tatagctgca atagcatctc gtttcatttg tataatcaat
    36841 tccggtttaa atatcagtgt gaacgtaagc acgacacaga ttaaaaacaa cactgccgga
    36901 atgagtcgtt tcaatcgtcg cttccaaaac tctagcaaat cgattttttg cgtccgataa
    36961 tactcactta tcaacaaact tgttattaaa taacctgaaa taacgaagaa tgtatctact
    37021 cctaaaaagc ccccacttaa ccattgtgca ttcaagtgat aaataatgat tcctataact
    37081 gcgaatgccc tcaatccatc taatccgggt aagtatcgcg gggaatacat tttttctaaa
    37141 cgtttaaagt cttttgtatc catgttaata aacgccccat ttatttttct ctattttgta
    37201 gcatatcata atatttttga aaataaaata ttgcactgat tttcattaat tgatttaacc
    37261 cttaattaag atagttttaa attttttatt aagtagaaaa caattattac agttaatttc
    37321 attactgcaa accacatata aatttgtcga ttttactaca taacatagat tatc
//
LOCUS       DAHHOJ010000015        34752 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.015, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000015 DAHHOJ010000000
VERSION     DAHHOJ010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34752)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34752)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34752
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.015"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            198..2447
                     /gene="pflB"
                     /locus_tag="NBH32_000641"
     CDS             198..2447
                     /gene="pflB"
                     /locus_tag="NBH32_000641"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCD1514271.1"
                     /translation="MLETNKNHATAWQGFKNGRWNRHVDVREFIQLNYTLYEGNDSFL
                     AGPTEATSKLWEQVMQLSKEERERGGMWDMDTKVASTITSHDAGYLDKDLETIVGVQT
                     EKPFKRSMQPFGGIRMAKAACEAYGYELDEETEKIFTDYRKTHNQGVFDAYSREMLNC
                     RKAGVITGLPDAYGRGRIIGDYRRVALYGVDFLMEEKMHDFNTMSTEMSEDVIRLREE
                     LSEQYRALKELKELGQKYGFDLSRPAENFKEAVQWLYLAYLAAIKEQNGAAMSLGRTS
                     TFLDIYAERDLKAGVITESEVQEIIDHFIMKLRIVKFARTPDYNELFSGDPTWVTESI
                     GGVGIDGRPLVTKNSFRFLHSLDNLGPAPEPNLTVLWSVRLPDNFKTYCAKMSIKTSS
                     IQYENDDIMRESYGDDYGIACCVSAMTIGKQMQFFGARANLAKTLLYAINGGKDEKSG
                     AQVGPNFEGINSEVLEYDEVFKKFDQMMDWLAGVYINSLNVIHYMHDKYSYERIEMAL
                     HDTEIVRTMATGIAGLSVAADSLSAIKYAQVKPIRNEEGLVVDFEIEGDFPKYGNNDD
                     RVDDIAVDLVERFMTKLRSHKTYRDSEHTMSVLTITSNVVYGKKTGNTPDGRKAGEPF
                     APGANPMHGRDQKGALSSLSSVAKIPYDCCKDGISNTFSIVPKSLGKEPEDQNRNLTS
                     MLDGYAMQCGHHLNINVFNRETLIDAMEHPEEYPQLTIRVSGYAVNFIKLTREQQLDV
                     ISRTFHESM"
     gene            2470..3225
                     /gene="pflA"
                     /locus_tag="NBH32_000642"
     CDS             2470..3225
                     /gene="pflA"
                     /locus_tag="NBH32_000642"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HCD1514272.1"
                     /translation="MLKGHLHSVESLGTVDGPGLRYILFTQGCLLRCLYCHNPDTWKI
                     SEPSREVTVDEMVNEILPYKPYFDASGGGVTVSGGEPLLQMPFLEKLFAELKENGVHT
                     CLDTSAGCANDTKAFQRHFEELQKHTDLILLDIKHIDNDKHIRLTGKPNTHILNFARK
                     LSDMKQPVWIRHVLVPGYSDDKDDLIKLGEFINSLDNVEKFEILPYHQLGVHKWKTLG
                     IAYELEDVEAPDDEAVKAAYRYVNFKGKIPVEL"
     gene            3561..5324
                     /locus_tag="NBH32_000643"
     CDS             3561..5324
                     /locus_tag="NBH32_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase
                     membrane domain-containing protein"
                     /protein_id="HCD1514273.1"
                     /translation="MKRISKDIWAVFKLLYQNKGRFSINALLLQLIMIFISSTYLILL
                     FNMMLKVAGQSQLTINNWMEIVSHPASVILLIIFILSVAFLIYVEFSLLVYMVYAGFD
                     RQIITFKSIFKNAFVNVRKLIGVPVIFFVIYLMLMIPIANLGLSSVLTKNIYIPKFLT
                     EELMKTTKGIIIYGTFMIAVFILNFKLIFTLPLTILNRQSLFKNMRLSWQITKRNKFR
                     LVIEIVILELIIGAILTLIISGATYLAICVDEEGDKFLVSSILFVVLKSALFFYYLFT
                     KLSLISVLVLHLKQENVLDQPGLEFKYPKPKRKSRFFIISMVLAVTCFIGYNMYLLYN
                     NTINTNISIIGHRGFEDKGVENSIPSLKAAAKANVEYIELDTIMTKDKQFVVSHDNNL
                     KRLTGVNKNISESNFKDVVGLKMRQNGHEAKLVSLDEFIETAKQSNVKLLVELKPHGK
                     EPADYTQRVIDILKKHGVEHQYRVMSLDYDVMTKLKKEAPYLKCGYIIPLQFGHFKET
                     SLDFFVIEDFSYSPRLVNQAHMENKEVYTWTINGEEDLTKYLQTNVDGIITDDPALAD
                     QIKEEKKDETYFDRSIRILFE"
     gene            complement(5488..5832)
                     /locus_tag="NBH32_000644"
     CDS             complement(5488..5832)
                     /locus_tag="NBH32_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCD1514274.1"
                     /translation="MTTQMKIKTYLVAGIKAALLDTTGIKLASKSETTSHTYQHQALV
                     DQLYELIANTDLNKLSYLNLDAFQKRDILAAHYIAKSAIRTKNLDQMTKAKHRLESIY
                     DSISNPLHSQKN"
     gene            6003..7991
                     /gene="coa"
                     /locus_tag="NBH32_000645"
     CDS             6003..7991
                     /gene="coa"
                     /locus_tag="NBH32_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015445709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylocoagulase"
                     /protein_id="HCD1514275.1"
                     /translation="MEELKNYEKQIISLGALAVASSLFTWDNKADAIVTKDYNGKSQV
                     KKESKNGTLIDSRYYWEKIEALEKQFSSALALTDEYQYGGNEYKEAKDKLMERILGED
                     QYLLKKKIDEYDYYKKWYKATYPNDNSKMYSFHKYNVYYLTMNEYNEITNSLKDAVEK
                     FNNEVRDIQSKNEDLKPYDENTEKQETDKIYEFVSEIDTVFAAYYSHEKFGIHAKELR
                     AKLDIILGDVHNPNRITNERIKKEMMEDLNSIVDDFFMETNQNRPTTIKKYDPNIHDY
                     TKKKENKENFDKLVKETREAVEKADESWKNKTVKKYEETVTKSPFVKEEKKVEEPQLP
                     KVGNQQEVKTTAGKAEETTQPLVKIPQGTITGEIVKGPDYPTMENKTLQGEIVQGPDF
                     PTMEQNRPSLSDNYTQPTTTNPILEGLEGSSSKLEIKPQGTESTLQGTQGESSDIEVK
                     PQATETTEASQYGPRPQFNKTPKYVKYRDAGTGIREYNDGTFGYEARPRFNKPSETNA
                     YNVTTNQDGTVTYGARPTQNKPSKTNAYNVTTHANGQVSYGARPTYKKPSETNAYNVT
                     THANGQVSYGARPTQNKASETNAYNVTTHANGQVSYGARPTQNKPSKTNAYNVTTHGN
                     GQVSYGARPTYKKPSETNAYNVTTHADGTATYGPRVTK"
     gene            complement(8173..8280)
                     /locus_tag="NBH32_000646"
     CDS             complement(8173..8280)
                     /locus_tag="NBH32_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514276.1"
                     /translation="MCVRTRLVSSSSVRLSKAIIIAVIVVYHLDVRGLF"
     gene            complement(8594..9778)
                     /locus_tag="NBH32_000647"
     CDS             complement(8594..9778)
                     /locus_tag="NBH32_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD1514277.1"
                     /translation="MQEAYIVAYGRSAAAKAKQGALFHERPDDVAAKVLQGVLKRIDG
                     KFNKNMIEDVIVGTAFPEGLQGQNIARTIALRTGLSDTVPGQTVNRYCSSGLQTIAIA
                     ANQIMAGQGDILVAGGVELMSAVPMGGNEPTNNPTLQYDDIGASYPMGLTAENVASQF
                     DVSREDQDAYAVRSHQRAYDAQRDGRFKNEIIPIQVNSVEYTNAGPKVHTNIFDQDEF
                     IRPDTTMEALAKLRTVFKADGTVTAGTSAPLSDGAGFVVLMSGDKVKELGVTPIARFV
                     GYKAVGVDPKIMGIGPAYAIPEVLSLSNLSVEDIDLIELNEAFASQTIASIKEVGLDI
                     SRTNVNGGAIALGHPLGATGAMLTARLLNEMGRRPDSRYGMVTMCIGVGMGAAAIFEY
                     VR"
     gene            complement(9808..12069)
                     /locus_tag="NBH32_000648"
     CDS             complement(9808..12069)
                     /locus_tag="NBH32_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HCD1514278.1"
                     /translation="MTINKVTVLGAGTMGAQLAALFVNAGLKVKLLDIVVDKNDPNLI
                     AKKSYDKITDKKRPLLFDLNLASHLTYGNFDDDLVNDDADLYIEAVKEDIEIKHAVWQ
                     QVLQHAKEDALFATNTSGIPINAIAQVFNEKDQERFFGLHFFNPPRIMKLVELIPTSH
                     TKESIILDVKNFAQNVLGKGVIVVNDVPGFVANRVGTQTMNDIMYRAEQHKLSIVDVD
                     ALTGQAIGRPKTGTYALSDLVGLDIAVSVIKGMQQVPEETPYFHDVKIVNTLFDNGAL
                     GRKTKQGFYKKDKETKARLVYDVEKQDYVPVSQPQLPILNEFNKDLAHNLDVIFNAQD
                     EAGLFLWETLRNNFYYSAINVPKATDDFRDIDRALVWGFNWKLGPFQLWDAMGYERVK
                     KRMEDEFGDLPQWISDLDGGFYKKDETIEYATPVSHFVKDELWDKGDAKLSVTHDDQL
                     LLKLQSKNNVITDEFNDALVDAIDLLENDHYTSMVIYADGNNFSVGANLFLMKKAHED
                     GLVDDVVAQSIDKLHYSFNRLKYSLKPVVTAVQGRALGGGCELVLYSPIVVAASETYI
                     GLVEAGVGLLPSGGGLAEMADRILRTSHKFDDKQASMTKVLTNIAFAKVSTNAFEARR
                     YGYLRDTDTIIFNTAQRVEVALKRAKYEAETNYIPNPRHQYIALGEDFKAFIQGQLDA
                     QRRGHFISDHDYHIALNIATILAGGDLPRNTFINQRYIQSLEKIGFIDLLKSKKSYER
                     IAHMLKTGKPLRN"
     gene            complement(12256..13467)
                     /locus_tag="NBH32_000649"
     CDS             complement(12256..13467)
                     /locus_tag="NBH32_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HCD1514279.1"
                     /translation="MTFEKETVLKTLFPEDVLSIAKGLTDGEVEFLQQVDSLLESKYR
                     ENINQHWIDATVPEDFFKNLGELNYFNNPLLYKDRPNAKMPSQLFQFFMSYLLARFDI
                     SLATLLGVHQGLGHNTFYFGGSKEQIAKYVPKLQSHELRTCFALTEPEHGSDVAGGLE
                     TVAERQGDTWVINGEKKWIGGAHVSDVIPVFAVNKETGKPHCFVVRPEQDGVDIEVID
                     NKIALRIVPNALIKLTNVKVDEADRLQNITSFKDIAKILYSTRAGVAYMATGGMAGAL
                     RATLDYVTERKQFGKPISKYQLIQEKLAMMQGNLAQAMATCAQLANMQAHGEYDEVAT
                     STAKMMNALRLRETVAMGRGITGGNGILADDYDIARFFSDAEAIYTYEGTHEINALVI
                     GRALTGDSAFV"
     gene            complement(13579..15084)
                     /locus_tag="NBH32_000650"
     CDS             complement(13579..15084)
                     /locus_tag="NBH32_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCD1514280.1"
                     /translation="MNFDWIKTRSDFDDDKPAVIDHAKQTSWTYQQLNARADNMAHYL
                     TSQGVKKGDVIGIFAPNDIAILDLLFACFKTGAVFLPLNWRLNPKEIAAIVEDAQLKL
                     LFYAEKHLSSLTDIDQNLLHMDIDVAQYDEIVNPDYHQPFQATPVEPQDLAALIYTSG
                     TTGSPKGVMFSYESFVHNGANLELTYKFNSNYITIVSTPMFHVLGFNDTVLPVLMSGG
                     TLILQRYFNGEELNDMIAQYHPTFIIMIPTMYYSTLRASNFNPENFKAMDYIIQGGSQ
                     PLPSIQAAFKQYGINIINGYGLTEAPLVLVNTPENSKRKPMSIGKAVMFVDARILDDN
                     GEEVPTGEIGELAIKAKNVTPGYWNKPAETAKAFHGRYLLTGDLAKMDDDGDIFIIDR
                     KKELIITGGENVLPSEVENALAEHPLVDRCVVVGYDHPKYGESIAAAIILREDEPHYA
                     EILDQHMRSRLAGYKVPRMYVPVTHMPLNSTQKPDKLAIRQMMNDKVSQTL"
     gene            complement(15110..16687)
                     /locus_tag="NBH32_000651"
     CDS             complement(15110..16687)
                     /locus_tag="NBH32_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HCD1514281.1"
                     /translation="MEVTCLKQITWHDLQHIIKDGDVIGLPALAVANLPAEVLRAVLA
                     QHDTYHTPNDLTFILANDIHSLGAAPDLDDFIERRMIKRVIMSILTASSKTAQAMKNN
                     DIEAYFLPQGIIATHYRQSNQLLPGVITKIGLNTAVDPRYGGGKVNTRTTDDLVSLVT
                     INDETYLHYTFPSVDVALLRGTYADQQGNIYLTQEAYLSECYHVALNTKANHGKVIVQ
                     VKALVDDYQLKPNEVVIPGNLVDYVYVTEDEKNHRQVIQSHYLPALSGEERIDGIPEP
                     ALPFNSRKLILRRAAQFLTYGDTISIGYGINNELSNLLHEECVEHDVQPILDVGIFGG
                     FVGSREHFGMNYNADVRMPHDRAWDFIYNNGVSVAYLSFAEVDQHGNVNVSYFNDRLN
                     GCGGFIDITQSVNKIIFSGTFVAGSHVSCHNQRLNIETEGQNQKFVSDVSHIDFNAQY
                     SQSLEQEVYFVTDRAVFELTNQGLKLIEIAPGLDLHKDILNQMAFKPIIADHLKLIDT
                     SIYKEKWGQLKQSIHKV"
     gene            17046..17717
                     /locus_tag="NBH32_000652"
     CDS             17046..17717
                     /locus_tag="NBH32_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001656934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1514282.1"
                     /translation="MRVNVLQHTPNEGVGYIGEWAKESGHEVYIYHPYMNDGVLPLAE
                     ETDMLVILGGPMNPDDEMPWIQSERELILVLMAQNKPIYGACFGAQQISKALGYAVLK
                     SPVKEVGWDDVYLESEIIPNIPSRLKALHWHEDMFQIPQQAELLFSGNHIRNQGFIID
                     HHVIGLQFHFEPGPDDVREIVLNDAQYINHSILEQSAEDILAFEVPKQNRQAIFRILD
                     FITQA"
     gene            17940..19082
                     /locus_tag="NBH32_000653"
     CDS             17940..19082
                     /locus_tag="NBH32_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family intramembrane metalloprotease"
                     /protein_id="HCD1514283.1"
                     /translation="MSSASTQSTKTSDIHNESIDKQMEAKAHETAQNTDLKNEARSLF
                     DNATKSIGRLAGNDESLNLNLKDMFSEVFKPHTKNEADEIFIAGTAKTTPAICDISEE
                     WGKPWLFSRVFIAFTVTFIGLWVMAAIFNNTNAIPGLIFIGALTVPLSGLFFFYESNA
                     FKNISIFEVIIMFFIGGVFSLLSTMVLYRFVVFSDQFERFGSLTFFDAFLVGLVEETG
                     KALIIVYFVNKLKTNKILNGLLIGAAIGAGFAVFESAGYILNFALGENVPLLDIVFTR
                     AWTAIGGHLVWSAIVGAAIVIAKEQHGFEFKDIFDKRFLIFFLSAVGLHGIWDTSLTV
                     LGSDTLKIFILIVIVWILVFILMGAGLKQVNLLQKEFKEQQKKVDE"
     gene            complement(19395..20870)
                     /locus_tag="NBH32_000654"
     CDS             complement(19395..20870)
                     /locus_tag="NBH32_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1514284.1"
                     /translation="MKFKRLATIFSAVLVLSGCGSMHSSGKDLNISLPLKTKSIAPYE
                     TDVPVKIGAAESLFKTNDQGKIEKVLVKSYHQPNDKTLDIELKDNIKFQNGQKLTAEK
                     VKSSLENSMKKSDLVKYSLPISSITAKGQKLTIKTNYAYPELVSELANPFMAIYDTDA
                     KSDVNQTPVGTGPYQIKDYKQSRKISLSNFKDYWQGKPKLDHITVTYQEDGNNRVRNL
                     ESQKDDLITDVPVNKVQDIENNQNLKVSKESGFRTSLLMYNHTNKKMTKSVREALDHI
                     IDRQGIADHIYQGYAKPATSPFNDKIPYIKEPKLTKQNIEQAKALLAKDGYTKEHPLK
                     IKLITYDGRPELSKIAQVLQSDAKKANIEIDIKSVDDIEGYLKDRSAWDATMYSFGTI
                     PRGDTGYFFNQAYKKDGAINKGDYNNSNVDDLINQLNHTVDIKERHNISNDIIKLSSR
                     DVPNSYIAYNDQIVAANSKVKNYKVTPEGIYLIDYRTTIER"
     gene            complement(21068..21424)
                     /locus_tag="NBH32_000655"
     CDS             complement(21068..21424)
                     /locus_tag="NBH32_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCD1514285.1"
                     /translation="MTVDIGRIYDNKDNTDAIRILVDRVWPRGISKRTANLDYWLKDI
                     APSTELRQWFQHDPKLFGAFKEKYEKELRDQDVQKEAFEKLKDIVNQHNHVLLLYSAK
                     DTKHNQAVVLQQLLNT"
     gene            complement(21581..21754)
                     /locus_tag="NBH32_000656"
     CDS             complement(21581..21754)
                     /locus_tag="NBH32_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514286.1"
                     /translation="MKLNQRYVKVFALYFVSIVTANIVVKNNNLIKTLIQTLAGYTVF
                     AVGLKYLTKRKNK"
     gene            complement(21780..22925)
                     /locus_tag="NBH32_000657"
     CDS             complement(21780..22925)
                     /locus_tag="NBH32_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide dioxygenase"
                     /protein_id="HCD1514287.1"
                     /translation="MLTEQEKDIIKQTVPLLKEKGTEITSIFYPKMFKAHPELLNMFN
                     QTNQKRGMQSSALAQAVMAAAVNIDNLSVIKPVIMPVAYKHCALQVYAEHYPIVGENL
                     LKAIQDVTGLEENDPVIQAWAKAYGVIADVFIQIEKEIYDQMMWIGFKPFKITNIKQE
                     SEDIKSFTVETEEYDFSEFTPGQYITVDVSSDKLPYRAKRHYSIVSGDKNHLTFGVKR
                     DVTTEHEGEVSTILHDEIKEGDMINLAAPVGGFVLENTTEPQLFLGSGIGVTPLVAMY
                     EAASAKGLDTQMVQVAENEQHLPFKDNFNSIASHHDNAKLYTHLKDKQGYIGAEELKA
                     FLANKPEIYICGGTKFLQSMIEALKSLNYDMDRVHYETFIPRLSVAV"
     gene            23080..23187
                     /locus_tag="NBH32_000658"
                     /pseudo
     CDS             23080..23187
                     /locus_tag="NBH32_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            23498..24451
                     /locus_tag="NBH32_000659"
     CDS             23498..24451
                     /locus_tag="NBH32_000659"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1514288.1"
                     /translation="MNKFKGNKVVLIGNGAVGSSYAFSLVNQSIVDELVIIDLDTEKV
                     RGDVMDLKHATPYSPTTVRVKAGEYSDCHDADLVVICAGAAQKPGETRLDLVSKNLKI
                     FKSIVGEVMASKFDGIFLVATNPVDILAYATWKFSGLPKERVIGSGTILDSARFRLLL
                     SEAFDVAPRSVDAQIIGEHGDTELPVWSHANIAGQPLKTLLEQRPEGKAQIEQIFVQT
                     RDAAYDIIQAKGATYYGVAMGLARITEAIFRNEDAVLTVSALLEGEYDEEDVYIGVPA
                     VINRNGIRNVVEIPLNDEEQSKFAHSAKTLKDIMAESEELK"
     gene            complement(24772..26301)
                     /locus_tag="NBH32_000660"
     CDS             complement(24772..26301)
                     /locus_tag="NBH32_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1514289.1"
                     /translation="MKSLFEKAQQFGKSFMLPIAILPAAGLLLGIGGALSNPNTVKAY
                     PILDITLLQNIFTLMSAAGSIVFQNLPVIFAIGVAIGLSRSDKGTAGLAALLGFLIMN
                     ATMNGLLTITGTLAKDQLAQNGQGMVLGIQTVETGVFGGITTGIMTAILHNKYHKVVL
                     PPYLGFFGGSRFVPIVTAFAAIFLGVLMFFIWPSIQAGIYHVGGFVTKTGAIGTFVYG
                     FILRLLGPLGLHHIFYLPFWQTALGGTLEVKGHLVQGTQNIFFAQLGDPDVTKYYSGV
                     SRFMSGRFITMMFGLCGAALAIYHTAKPEHKKVVGGLMLSAALTSFLTGITEPLEFSF
                     LFVAPILYVIHAFFDGLAFMMADIFNITIGQTFSGGFIDFLLFGVLQGNSKTNYLYVI
                     PIGIVWFCLYYIVFRFLITKFNFKTPGREDKAAAQQVEATERAQTIVAGLGGQDNIEI
                     VDCCATRLRVTLHQNDKVDKVLLESTGAKGVIQQGTGVQVIYGPHVTVIKNEIEELLG
                     D"
     gene            26663..27598
                     /locus_tag="NBH32_000661"
     CDS             26663..27598
                     /locus_tag="NBH32_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD1514290.1"
                     /translation="MKRKIIMDCDPGHDDAIALILAGAIDSPLEILAVTTVAGNQSVD
                     KNTTNALNVLDIMGRQDIAVAKGADRPLIKSAAFASEIHGESGLDGPKLPSTPSRQAV
                     AMPASDMIINKVMTSDTPVTIVATGPLTNVATALIREPRIAEHIESITLMGGGTFGNW
                     TPTAEFNIWVDAEAAKRVFESGITINVFGLDVTHQVLADDLVIERFESINNPVAQFVV
                     ELLQFFKKTYKTHFNMDGGPIHDACTILYLLQPELFTMVPVNIDIEHQSPLTYGTMAV
                     DLNHVTGKPANAYFATAVDVEEVWNLIDYKLRTYE"
     gene            27708..27805
                     /locus_tag="NBH32_000662"
                     /pseudo
     CDS             27708..27805
                     /locus_tag="NBH32_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498807.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            27930..30026
                     /locus_tag="NBH32_000663"
     CDS             27930..30026
                     /locus_tag="NBH32_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD1514291.1"
                     /translation="MNGDNQQILREIVLNPTIHGKELESIFGLSRRQLGYRIQKINLW
                     LEQEGYPKLERTSQGNFIVSSEIMTLFKRDVSEQQMLNGNNVIFSIETRRYYLMLMLF
                     SKENAMSLNHFSIDLQVSKNTVIHDINHVKEQLENHGLSLKYSRKHGYEIVGDEFEVR
                     RFFIKLIDQRLNHDITKSEVLKALNLTFEDIAYQKDKIKQVEQFLKSRFIDKSLSSLP
                     YVLCVIRRRIQSGHVMNPLNINYQYLRDTKEYQATEIMTQHEPDLPEAEKLYLTLHLL
                     STSVQWTDLQESDSISNLTMAIAQMIHHFEQITFINIEDKEKLSQQLLLHLTPAFYRI
                     KYNLTDRDELINPLQGNYQSLFHMVKQSCQSLTEYFGKSLPDNEIAYLTMLFGGSLRR
                     QDENFDGKIKAIIVCTQGTSVSQMMLYELRNLFPEIIFLDAISLRTFENYTLDYDIVF
                     SPMFVLTHKKLFITKVALSENEQRKLRKEVMKYINKESADIDKEINKLMALIERTTTV
                     NDITELRDGLEDFIANYNSISTINGSIVTQNKTLDLADLIPARHVKRIHHVENIDEAI
                     AKASDVLVANHFIDIKYIHEMQQVFDDSYMVIMQNIAIPHAYSEKHVHKTAMSMLILQ
                     EPIYMSDGTAIHIIVPIAAVDKVTHLRALLQLRDVAQDNDAIKRIIQSRKNSDVNEIL
                     KNYSNKEARENGWDSN"
     gene            30011..30478
                     /locus_tag="NBH32_000664"
     CDS             30011..30478
                     /locus_tag="NBH32_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1514292.1"
                     /translation="MGQQLVHKENIMLNLSATDKESVLSQMSDVLFQNGFVKSTFKDA
                     VIDREKEFATGLPTHLCSVAIPHTDVEHINHRTIGVAVLEKEVPFIEMGTLDQQTEVK
                     IVFMLAMDKVDDQLKLLQQLMQIFQSEEKLEQILRTKDETILATLINDYLEYN"
     gene            30501..30779
                     /locus_tag="NBH32_000665"
     CDS             30501..30779
                     /locus_tag="NBH32_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCD1514293.1"
                     /translation="MKQVLVACGAGIATSTVVNNAIEEIAKEHNIKVDIKQIKITEVG
                     PYEDTADLLVTTAMTKKEYKFPVINARNFLTGIGIEETKQQILTELQK"
     gene            31006..32274
                     /locus_tag="NBH32_000666"
     CDS             31006..32274
                     /locus_tag="NBH32_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCD1514294.1"
                     /translation="MSYFTDFVRGFLDLGATVILPVVIFLLGLFFRQKIGAAFRSGLT
                     IGVAFVGIFLVIDLLVKNLGPAAQAMVKNLGVSLNVIDVGWPATSSIAWASSVAAFII
                     PLGIIVNVVLLVTKVTKTMNVDIWNFWHYTFTAAMVYAVSGSIWQALLAAVIFQVICL
                     KVADWTAPMMSEFFDLPGVSIATGATGSTISYAPGIYLVKLLQKVPGLNKLDADPETI
                     QKRFGAFGESIFVGLILGLGIGVLAGYKPGDIINLGMSMAAVMVLMPRMVKILMEGLM
                     PVSESARTWLNKRFGEREIYIGLDAAVALGHPAVISTALILVPITVLLAVILPGNQVL
                     PFGDLATIPFVVAFIVGAARGNIIHSVIVGTIMIAISLYIATDVAPIFTDMAKGTNVQ
                     MPKGSSEISSIDQGGNIVNYLIFKLFSLFN"
     gene            32292..33347
                     /locus_tag="NBH32_000667"
     CDS             32292..33347
                     /locus_tag="NBH32_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCD1514295.1"
                     /translation="MKALVKTREGHGNLELLDKEVATPLDDKVKIKVHYAGICGTDIH
                     TYEGHYKVNFPVTLGHEFSGEIVEVGADVKDFKVGDRVTSETTFYICNECEYCESKDY
                     NLCNHRKGIGTQVDGAFTNYVIAREESLHHIPDEVSYQSAAMTEPLACAHHGVSKIQV
                     NSGDVAVVMGPGPIGLLVAQVLKSKGATVVVTGLDNDKVRLDKAEALHMDYVVNLQQT
                     DLKTYINGITDGYGADVVVECSGAVPAARQGLDILRKKGFYSQIGIFKDAEIPFDMEK
                     VIQKEITVVGSRSQKPADWEPSLRLMADGLVNAEALVTKIYDISKWDEAYQHLKSGEG
                     IKALLKPLDLDENEGEN"
     gene            33349..33495
                     /locus_tag="NBH32_000668"
     CDS             33349..33495
                     /locus_tag="NBH32_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514296.1"
                     /translation="MVESMLTFMLGPLRQITDFYMEHLLVSNSIVIAGYFATGIFKKK
                     KVVN"
     gene            33519..34562
                     /locus_tag="NBH32_000669"
     CDS             33519..34562
                     /locus_tag="NBH32_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1514297.1"
                     /translation="MKALKLYGVEDLRYEDNDKPVIESANDVIVKVRATGICGSVTSR
                     YKKMGPYIQGMPFGHEFSGVVDAIGSDVTHVKVGDKVTGCPAIPCYQCEYCLKGEYAR
                     CEKLFVIGSYEPGSFAEYVKLPAQNVLKVPDNVDYIEAAMVEPSAVVAHGFYKSNIQP
                     GMTVAVMGCGSIGLLAIQWARIFGAAHIIAIDIDAHKLDIATSLGAHQTINSKEEDLE
                     KFIENHYANQIDLAIESSGAKVTIGQILTLPKKGGEVVLLGIPYDDIEIDRVHFEKIL
                     RNELTVCGSWNCLSSDFPGKEWTATLHYMKTKDINVKPIISHFLPLEKGPETFDKLVN
                     KKERFDKVMFTIY"
BASE COUNT        11616 a         5776 c         6398 g        10962 t
ORIGIN      
        1 ataactgttg tgtaacagaa tgcaattagc atattactgt tacacaaatt agtacagttt
       61 ctatgttttg acatacattt gatgaaaatt gtacataatt tatgtgaaaa aaatcacaac
      121 aaacatgcta caatgactat gaaaacgtta acatagcatt tcaaattcac aacattatac
      181 agatggaggc gtttagtatg ttagaaacaa ataaaaatca tgcaacagct tggcaaggat
      241 ttaaaaatgg aagatggaac agacacgtag atgtaagaga gtttatccaa ttaaactaca
      301 ctctttatga aggtaatgat tcatttttag caggaccaac agaagcaact tctaaacttt
      361 gggaacaagt aatgcagtta tcgaaagaag aacgtgaacg tggcggcatg tgggatatgg
      421 acacgaaagt agcttcaaca atcacatctc atgatgctgg ttatttagac aaagatttag
      481 aaacaattgt aggtgtacaa actgaaaagc cattcaaacg ttcaatgcaa ccattcggtg
      541 gtattcgtat ggcgaaagca gcttgtgaag cttacggtta cgaattagac gaagaaactg
      601 aaaaaatctt tacagattat cgtaaaacac ataaccaagg tgtattcgat gcatattcta
      661 gagaaatgtt gaactgccgt aaagcaggtg taatcactgg tttacctgat gcatacggac
      721 gtggacgtat tatcggtgac tatcgtcgtg tagctttata tggtgtagat ttcttaatgg
      781 aagaaaaaat gcacgacttc aacacgatgt ctacagaaat gtcagaagat gtaattcgtt
      841 tacgtgaaga attatcagaa caatatcgtg cattaaaaga attaaaagaa cttggacaaa
      901 aatatggttt cgatttaagc cgtccagcag aaaacttcaa agaagcagtt caatggttat
      961 acttagcata ccttgctgca attaaagaac aaaacggtgc agcaatgagt ttaggtcgta
     1021 catcaacatt cttagatatc tatgctgaac gtgaccttaa agcaggcgtt attactgaaa
     1081 gcgaagttca agaaattatt gaccacttca tcatgaaatt acgtattgtt aaatttgctc
     1141 gtacacctga ttacaatgaa ttattctctg gagacccaac ttgggtaact gaatctatcg
     1201 gtggtgtagg tattgacgga cgtccacttg ttacgaaaaa ctcattccgt ttcttacact
     1261 cattagataa cttaggtcca gcaccagaac caaacttaac agtattatgg tcagtacgtt
     1321 tacctgacaa cttcaaaaca tactgtgcaa aaatgagtat taaaacaagt tctatccaat
     1381 atgaaaatga tgacattatg cgtgaaagct atggcgatga ctatggtatc gcatgttgtg
     1441 tatcagcgat gacaattggt aaacaaatgc aattcttcgg tgcacgtgcg aacttagcta
     1501 aaacattact ttacgctatc aatggtggta aagatgaaaa atctggtgca caagttggtc
     1561 caaacttcga aggtattaac agcgaagtat tagaatatga cgaagtattc aagaaatttg
     1621 atcaaatgat ggattggcta gcaggtgttt acattaactc attaaatgtt attcactaca
     1681 tgcacgataa atacagctat gaacgtattg aaatggcatt acatgataca gaaattgtac
     1741 gtacaatggc aacaggtatc gctggtttat cagtagcagc tgactcatta tctgcaatta
     1801 aatatgcaca agttaaacca attcgtaacg aagaaggtct tgtagtagac tttgaaatcg
     1861 aaggcgactt ccctaaatac ggtaacaatg acgaccgtgt agatgatatc gcagttgatt
     1921 tagtagaacg ctttatgact aaattacgta gtcataaaac atatcgtgat tcagaacata
     1981 caatgagtgt attaacaatt acttcaaacg ttgtatacgg taagaaaact ggtaacacac
     2041 cagacggacg taaagctggc gaaccatttg caccaggtgc aaacccaatg catggccgtg
     2101 accaaaaagg tgcattatct tcattaagtt ctgtagctaa gatcccttac gattgctgta
     2161 aagatggtat ttcaaataca ttcagtatcg taccaaaatc attaggtaaa gaaccagaag
     2221 atcaaaaccg taacttaact agtatgttag atggttacgc aatgcaatgt ggtcaccact
     2281 taaatattaa cgtatttaac cgtgaaacat taatagatgc aatggaacat ccagaagaat
     2341 atccacagtt aacaatccgt gtatctggtt acgctgttaa cttcattaaa ttaacacgtg
     2401 aacaacaatt agatgtaatt tctcgtacat tccatgaaag tatgtaacaa aatttaaggt
     2461 gggagcacta tgcttaaggg acacttacat tctgtcgaaa gtttaggtac tgtcgatgga
     2521 ccgggattaa gatatatatt atttacacaa ggatgcttac ttagatgctt gtattgccac
     2581 aatccagata cttggaaaat tagtgagcca tcaagagaag tcacagttga tgaaatggtg
     2641 aatgaaatat taccatacaa accatacttt gatgcatcgg gtggcggtgt aacagtcagt
     2701 ggtggcgaac cattgttaca aatgccattc ttagaaaaat tatttgcaga attaaaagaa
     2761 aatggtgtgc acacttgctt agacacatcg gctggatgtg ctaatgatac aaaagcattt
     2821 caaaggcatt ttgaagaatt acaaaaacat acagacttga tattattaga tataaaacat
     2881 attgataatg acaaacatat tagattgacg ggaaagccta atacacacat ccttaacttc
     2941 gcgcgcaaac tgtcagatat gaaacaacct gtatggattc gacatgtcct tgtgcctggt
     3001 tattctgatg ataaagacga tttaattaaa ctaggggaat ttattaattc tcttgataac
     3061 gtcgaaaagt ttgaaattct gccatatcat cagttaggtg ttcataagtg gaaaacattg
     3121 ggcattgcat atgaattaga agatgtcgaa gcgcccgatg atgaagctgt taaagcagcc
     3181 taccgttatg ttaacttcaa agggaaaatt cccgttgaat tataaataca attcagaccg
     3241 aaaagaaagc atatgtaact tcaagagtga aggggcatat gcttcttttt caattgagta
     3301 tagagtaata gcaagacgta gtaggtatat gagacaactt ctacaatggt tgaaggaaga
     3361 catttttgta agtagctatg ctgataaaga atgtgaagcc ttgttaaagg tggggttcca
     3421 atatcatcat ttagctgatg ttgaatgggt tattatttgc tacttgcata tgaatatgag
     3481 tcttttcaaa tttttattga ccctgagtaa tgaaaaatat taagatgaaa cttaatatta
     3541 aagcaatgcg gagcgtgatt atgaagagaa ttagtaaaga tatatgggca gtatttaaat
     3601 tactgtatca aaataaaggg cgttttagca ttaacgcctt actattgcag ttaatcatga
     3661 tttttattag tagtacatac ttaattttac tatttaatat gatgttaaaa gtagctgggc
     3721 aaagccaact tacgattaac aattggatgg aaatcgtaag tcaccctgcc agtgtgatac
     3781 ttcttattat attcatatta agtgttgcct ttctgattta tgtagagttt tcattgttag
     3841 tttatatggt ttatgccggc tttgatcgac agattattac atttaaatcc atttttaaaa
     3901 atgcctttgt aaatgtgcgt aaactcatag gtgtaccagt tattttcttt gtcatttatt
     3961 taatgttaat gatacccatt gctaacctag gactaagttc agtattaaca aaaaatattt
     4021 acatacctaa atttttaacg gaagaactta tgaaaacgac aaaaggtata atcatttacg
     4081 gtacctttat gattgctgta tttatattaa actttaaatt aatatttact ctaccgttaa
     4141 cgattttaaa ccgccagtcg ttatttaaaa atatgagact aagttggcaa attacgaagc
     4201 gaaataagtt tcgacttgtt atagaaatag ttatattgga actcatcatt ggtgcgattt
     4261 taacattaat tatttcagga gcaacatatc ttgctatttg tgtagatgaa gaaggagata
     4321 agtttttagt ctcatcaatt ttatttgttg tattgaaaag cgcattgttc ttctattatt
     4381 tatttacgaa attatcatta atcagtgtgt tagtactgca cttaaaacaa gagaatgtat
     4441 tagaccaacc gggcttagaa tttaaatacc caaaaccgaa acggaaatct aggttcttta
     4501 taatttcaat ggtgcttgca gtgacatgtt ttatcggtta taacatgtac ttactttaca
     4561 ataatactat caatacaaat atatccatta ttggccatcg tggtttcgaa gataaaggcg
     4621 ttgaaaattc tattccgtca ttgaaagctg ctgcaaaagc gaatgtcgaa tatattgagt
     4681 tagatacaat tatgacgaaa gataaacaat ttgttgttag tcatgataac aatttgaaac
     4741 gtttaacagg tgttaataaa aacatttctg aatctaattt caaagatgtc gtcggtttga
     4801 aaatgcgtca aaatggacat gaagcaaaac tggtatcctt agacgaattt attgaaacgg
     4861 ctaaacaatc aaatgtgaag ctactagtag agttaaagcc acatggtaaa gaaccagcag
     4921 attatacaca acgtgttatt gatattttga aaaagcatgg tgttgaacat caatatcgtg
     4981 tgatgtcatt ggattatgat gtgatgacta agttaaaaaa agaagcgcca tatctcaagt
     5041 gtggttatat catcccgtta cagtttggtc attttaaaga aacatcatta gatttctttg
     5101 tcatcgaaga tttttcttat tcgccaagac ttgttaatca agcacacatg gaaaataaag
     5161 aagtctatac ttggaccatt aacggcgaag aagatttaac gaaatactta caaaccaatg
     5221 ttgatggtat tatcacagat gacccagcat tagctgatca gattaaagaa gaaaagaaag
     5281 acgaaacata cttcgatcgt tctataagaa ttttgtttga ataatataaa caaagacctc
     5341 taaagttatc aagatgatac cttcagaggt ctttttaaca ttgtcaccta tgggataggc
     5401 aatcgtttca ttcgtttata atcatatgac aaggatttat aaggaaattt ggcgtcacaa
     5461 acacttacat gatttattgg tgaattatta atttttttgt gaatgcaaag ggttagaaat
     5521 tgaatcgtaa atactttcta atctatgttt cgctttagtc atttgatcca aatttttagt
     5581 gcgtatagct gattttgcaa tatagtgcgc agctaaaata tcgcgttttt gaaacgcatc
     5641 taaatttagg tacgataatt tatttaagtc agtgtttgct attaattcat ataattgatc
     5701 tacaagcgct tgatgttgat acgtatgtga tgtagtttca gatttgcttg ctaatttaat
     5761 accagtcgta tcaaggagcg ccgctttaat accagcaact aaatatgttt tgattttcat
     5821 ttgtgttgtc atgctttgtt actcctttga tgtacattaa tcaaaaaaat tatacactat
     5881 tgtatattgc aaagctaatt aactataaca aaaagatagt taatgctttg tttattctag
     5941 ttaatatata gttaatgtct tttaatattt tgtttcttta atgtagattg ggcaattaca
     6001 ttttggagga attaaaaaat tatgaaaagc aaataatttc gctaggcgca ttagcagttg
     6061 catctagctt atttacatgg gataacaaag cagatgcgat agtaacaaag gattataatg
     6121 ggaaatcaca agttaaaaaa gagagtaaaa atgggacatt aatagatagc agatattatt
     6181 gggaaaaaat agaagctcta gaaaagcagt tttcaagtgc attggcatta acagatgagt
     6241 atcaatatgg tggtaatgaa tataaagagg caaaagataa attaatggaa aggatattag
     6301 gagaagatca atatttatta aagaaaaaaa tagatgaata cgattattat aaaaaatggt
     6361 ataaagcaac ttacccaaac gataattcaa aaatgtattc atttcataag tataatgttt
     6421 actatttaac aatgaatgaa tataatgaaa tcacaaattc attaaaagac gctgtggaaa
     6481 aatttaataa tgaagtaaga gatattcaat ctaaaaatga agacttaaag ccttatgacg
     6541 aaaatactga aaaacaagaa actgataaaa tatatgaatt tgttagtgaa atagatacag
     6601 tttttgcagc gtattatagt catgaaaaat ttggtataca tgctaaagaa ttacgagcaa
     6661 aattagacat aatactaggt gacgtgcata acccgaatag aatcactaat gaacgtataa
     6721 aaaaagaaat gatggaagac ttaaattcaa tagtcgatga tttctttatg gaaacaaatc
     6781 agaatagacc tacaacaata aagaaatatg accccaatat tcatgattat acaaagaaaa
     6841 aagagaataa agaaaatttt gataagttag ttaaagaaac aagagaagca gttgaaaaag
     6901 cagacgaatc ttggaaaaat aaaactgtca aaaaatacga ggaaactgta acaaaatctc
     6961 cttttgtaaa agaagagaag aaagttgaag aacctcaatt acctaaagtt gggaaccagc
     7021 aagaggttaa aactacggct ggtaaagctg aagaaacaac acaaccatta gttaaaattc
     7081 cacagggcac aattacaggt gaaattgtaa aaggtccaga ctatccaact atggaaaata
     7141 aaacgttaca aggtgaaatc gttcaaggtc cagatttccc aacaatggaa caaaacagac
     7201 catctttaag cgataattat actcaaccga cgacaacgaa ccctatttta gaaggtcttg
     7261 aaggtagctc atctaaactt gaaataaaac cacaaggtac tgaatcaacg ttacaaggta
     7321 ctcaaggaga atcaagtgat attgaagtta aacctcaagc aactgaaaca acagaagctt
     7381 ctcaatatgg tccgagaccg caatttaaca aaacacctaa atatgttaaa tatagagatg
     7441 ctggtacagg tatccgtgaa tacaacgatg gaacatttgg atatgaagcg agaccaagat
     7501 tcaataagcc atcagaaaca aacgcataca acgtaacgac aaatcaagat ggcacagtaa
     7561 catatggcgc tcgtccaaca caaaacaagc caagcaaaac aaacgcatat aacgtaacaa
     7621 cacatgcaaa cggtcaagta tcatacggag ctcgtccgac atacaagaag ccaagcgaaa
     7681 cgaatgcata taacgtaaca acacatgcaa acggtcaagt atcatatggc gctcgtccga
     7741 cacaaaacaa ggcaagcgaa acaaatgcat acaacgtaac aacacatgca aatggtcaag
     7801 tatcatatgg cgctcgtccg acacaaaaca agccaagcaa aacaaacgca tataacgtaa
     7861 caacacacgg aaatggccaa gtatcatacg gagctcgccc gacatacaag aagccaagcg
     7921 aaacaaatgc atacaatgta acaacacatg cagatggtac tgcgacatat gggcctagag
     7981 taacaaaata agtttgtaac tctatccaaa gatatacagt caatacaaaa cattacgtat
     8041 ctttacaaca gtaatcatgc attctatgat gcttctaact gaattaaagc atcgaacaat
     8101 cggaagcata tttctaaatt atttattcat tatagtctta aacataacat gacctaatat
     8161 attactaacc tattaaaata aaccacgcac atctaagtga tatacgacaa tcacagcaat
     8221 aataattgct ttagaaagtc gtaccgaact ggaacttaca agtctagttc gaacacacac
     8281 tgatgtgagt ggttttcttt attttaaaca tgaacaatca gataagttac tagcattagc
     8341 aaatattatt aaatcaaagg gcttcgattc ataaaattta aaacaatgat taaaattaga
     8401 cgtgtaaatg ttaaattcta aaacggaaat acccaccatc ccattaaacc acttttttgt
     8461 tcaatcacta tgtttcacat agcttcatta ataaaacgaa attgcttcaa cccgcttcaa
     8521 cttcaacctg cttcaacttc agcctacttc atttaataac aaaacgaatc cgcttcatcc
     8581 aaaatcaacc attctaacgc acatactcaa atatagcagc tgcacccata ccgacaccaa
     8641 tacacatcgt aaccatgccg taacggctat cgggacgtct acccatttca ttaagtaaac
     8701 gcgcggttaa cattgcgcct gtagcaccta atggatgacc taaagcaata gcgccaccat
     8761 tcacattcgt acgtgatata tctagaccta cttctttaat agatgcaatc gtttgagaag
     8821 caaatgcttc gttcaattcg atcaaatcaa tatcttcaac agatagattg ctgagtgaca
     8881 atacttcagg aatcgcatat gcaggcccaa tacccataat tttcgggtca acgcctactg
     8941 ccttataacc aacgaatcgt gcaataggtg tcacgccgag ttctttcact ttatctccag
     9001 acattaaaac tacaaatcct gcaccatcag aaagtggggc agatgttccc gcagtcacag
     9061 tgccgtcagc tttaaatact gtacgtaatt tggctaatgc ctccatcgtg gtgtcaggtc
     9121 gtataaattc atcttggtca aagatatttg tgtgtacttt tggtcctgcg tttgtatatt
     9181 caactgagtt tacttgtatt ggaataattt catttttgaa ccgaccatca cgttgtgcgt
     9241 cataggcacg ttgatgactt ctgacagcat aagcatcttg atcttcgcgt gatacgtcaa
     9301 attgggatgc tacattttca gcagttaaac ccataggata tgacgcacct atatcatcat
     9361 attgtaaggt gggattgttt gtgggctcgt tgccacccat tggtacggca ctcatcaatt
     9421 caacgccacc agctacaagt atatctcctt gaccagccat aatttgattg gctgcaatcg
     9481 cgatggtttg taatcctgat gagcagtagc gattcactgt ttgacccggt accgtgtcag
     9541 ataatcccgt acgcaatgca atcgttcgtg caatgttttg tccttgtaat ccttctggaa
     9601 aagccgtacc aacaatgaca tcttcaatca tattcttatt gaattttccg tcaatacgtt
     9661 tcaatacgcc ttgtaatact ttggctgcga catcatcagg tctttcgtgg aataatgcgc
     9721 cttgctttgc tttcgctgcg gctgaacgcc cataagctac aatgtatgct tcttgcatgg
     9781 ttatcatcct ctcttaatga ctatctttta attacgtaat ggcttaccag tttttaacat
     9841 atgtgcaatt ctttcatatg attttttaga ttttagtaag tcaataaagc caattttctc
     9901 caacgattga atgtaacgtt gattgataaa tgtatttctt ggtaaatcac cacccgctaa
     9961 aattgtggca atatttaagg cgatatgata atcatggtcg ctaataaaat gaccccgtct
    10021 ttgcgcatct aattgtcctt ggataaatgc tttgaagtct tcacctaaag cgatatattg
    10081 atgtctagga ttcggaatat agtttgtttc tgcttcatat ttcgcacgtt tgagcgcaac
    10141 ttcgacacgt tgtgctgtat tgaaaataat cgtatctgta tcacgtaaat aaccataacg
    10201 acgtgcctca aaggcatttg tagagacttt cgcaaatgcg atattcgtca gtacttttgt
    10261 catggaagct tgtttgtcat caaacttatg cgatgtgcgt aatatgcgat cagccatttc
    10321 tgcaaggcca ccgccactcg gtaataagcc aacgcctgct tcaacaagac cgatatacgt
    10381 ttcacttgca gcgacaacaa taggtgagta aagtacaagc tcacagccac cgcctaaggc
    10441 acgaccttga acagctgtga ctactggttt caaactatac ttcaaacgat taaagctata
    10501 atgtaattta tcaattgatt gtgcaacgac atcatctaca agaccgtctt catgcgcctt
    10561 tttcattaag aaaaggttag cacccacact gaaattgtta ccatctgcat aaataaccat
    10621 acttgtgtaa tggtcatttt ccagtaaatc aatcgcatca actaacgcat cgttgaattc
    10681 atcggtaatg acattatttt tactttgtaa tttcagtaac agttgatcat catgagttac
    10741 ggaaagtttg gcatcacctt tatcccaaag ttcatctttt acgaagtgag aaacaggtgt
    10801 tgcatattcg atggtctcat cttttttata aaagccacca tctaaatcac taatccattg
    10861 tggtaagtct ccaaattcgt cttccatacg ttttttaaca cgttcatatc ccattgcatc
    10921 ccataattgg aatggaccaa gtttccagtt gaacccccag acaagcgcac ggtctatgtc
    10981 tcggaaatca tcggtagctt taggtacatt gatagcagag taatagaaat tattacgtaa
    11041 tgtctcccat aaaaatagtc ccgcttcgtc ttgcgcattg aatatgacat caaggttatg
    11101 cgctaagtct ttattaaatt catttaaaat tggtaattgt ggttgcgata caggtacata
    11161 atcttgtttt tcaacatcgt aaacaagtcg agctttagtt tctttatcct ttttgtaaaa
    11221 tccttgtttc gttttacgtc cgagtgcgcc attgtcaaac aacgtattta caattttgac
    11281 atcatgaaaa taaggtgttt cttcaggtac ttgttgcatg cctttaatta cagacactgc
    11341 aatatctaaa ccgactaggt cagatagcgc atatgtacct gttttaggac gaccaatcgc
    11401 ttgtccagtt aaagcatcca catctacaat gcttaacttg tgttgctcgg cgcgatacat
    11461 aatatcattc attgtttgcg tgccgactct atttgcgaca aagccaggta catcattgac
    11521 gacaatgaca cctttaccta acacattttg cgcgaaattt tttacatcta atatgataga
    11581 ttccttcgtg tgtgacgtag gtattaactc cactaatttc ataatacgtg gtgggttaaa
    11641 gaaatgtaga ccaaagaatc gttcttgatc cttctcgtta aatacttgag caatcgcatt
    11701 aattggaata cctgatgtat ttgtagcgaa taaagcatct tctttagcat gttgtagaac
    11761 ttgttgccaa acagcatgct taatttcaat atcttctttg actgcttcga tatataaatc
    11821 agcatcatca tttaccaagt catcatcaaa attaccatat gttaaatgac tcgctagatt
    11881 taagtcgaat agtagcggcc gtttcttatc tgtaatttta tcgtaagatt ttttcgcaat
    11941 gagatttgga tcgtttttgt ctactacaat atctaatagt tttactttaa gtccagcatt
    12001 cacaaagagt gctgccagtt gagcgcccat tgtgcctgcg ccaagaacgg ttactttatt
    12061 aattgtcata gtgattcctc caatttagtt gaggataaga taaccattaa gataattgga
    12121 ataacgttgc tattttataa aattaattag gtatctttga cagtcatctt agcctcttat
    12181 ttaaggaaaa agctttatgc ttaaaataag tctgttttta gtgaaattac tgtatcttat
    12241 ataattcttt gctatttata cgaaagcaga atctccagtc aaagcgcgtc caattactaa
    12301 ggcattaatt tcatgtgtac cttcgtacgt gtaaatcgct tctgcatcag agaagaaacg
    12361 tgcaatatca taatcgtcag ctagtatgcc attaccacct gtaataccgc ggcccatagc
    12421 tactgtctca cgcaaacgta aggcattcat catcttcgcc gttgaagttg caacctcgtc
    12481 atattcacca tgtgcttgca tattagctaa ttgagcacat gttgccattg cttgagctaa
    12541 attaccttgc atcattgcta gcttttcttg tattaactga tatttactaa ttggtttgcc
    12601 gaattgctta cgctcagtga cataatctaa tgtggcacgt aaagcgccag ccataccacc
    12661 tgtagccata taagcaacgc ctgctctcgt tgaataaaga attttggcaa tatctttaaa
    12721 gcttgttatg ttttgtaagc gatccgcttc atctactttg acattagtta atttaattag
    12781 tgcgttagga acaatgcgaa gtgcgatttt attatcaatg acttcaatat cgacgccatc
    12841 ttgttctggt ctgactacaa agcaatgggg tttgccagtt tctttattta ctgcgaatac
    12901 tggaatgaca tcagatacat gtgcaccacc aatccatttc ttttcaccat tgataaccca
    12961 agtatcgccc tggcgttcag cgactgtttc aagacctccc gcaacgtccg aaccgtgttc
    13021 tggttcagtt aaagcaaagc atgtacgcag ttcatgtgac tgtaatttag gtacatattt
    13081 cgcaatttgt tctttgctac ctccgaaata gaaagtgtta tgccctaaac cttggtgaac
    13141 accgagtagg gtagctaagg aaatatcaaa tcgcgcgagt aggtaagaca tgaaaaactg
    13201 aaatagttga ctaggcattt tggcgtttgg acgatccttg taaagtaatg gattgttaaa
    13261 ataatttaat tctcccagat ttttaaaaaa gtcctcgggt acagtagcgt ctatccaatg
    13321 ttgattaata ttttcacggt acttactttc tagcaatgaa tctacttgtt gtaaaaattc
    13381 gacttcaccg tctgttaaac ctttagcaat actaagtaca tcttcaggaa ataatgtttt
    13441 taagactgtt tctttttcaa atgtcatata aattcctcct aaaaataata tgaatactaa
    13501 tgtgaaatgc atttaattca aaaacaacac gctttatttg taaacgctta cactaaatac
    13561 taaaaatttt tatcaccttt aaagtgtttg tgagactttg tcattcatca tttgtcgaat
    13621 cgcaagttta tctggtttct gcgtactgtt taacggcata tgtgtcactg gtacatacat
    13681 tcttgggact ttataacctg ctaaacgact tcgcatatgt tgatctaaaa tttcagcgta
    13741 atgaggttca tcttcgcgaa gtataatggc tgcagcaatt gattcaccat attttgggtg
    13801 atcatagcca acgaccacac accggtctac tagtggatgc tcagctaaag cattttcgac
    13861 ttcggatggt aagacatttt cgccaccagt tatgattaat tcttttttgc ggtcaataat
    13921 aaatatatcg ccatcgtcgt ccatcttcgc taagtcacca gttaataaat aacgaccatg
    13981 aaatgctttg gcagtctctg ctggtttatt ccaatatcct ggcgtgacat ttttagcctt
    14041 aattgcaagt tcgccaatct caccagtagg tacttcctca ccgttatcat caaggatacg
    14101 tgcatcaacg aacatgactg ctttaccaat actcattggc ttacgttttg aattttccgg
    14161 tgtattaaca agtacaagag gtgcttcagt taaaccatag ccgttaataa tgtttatgcc
    14221 atattgttta aaagctgctt ggatacttgg taatggttgt gaaccacctt ggatgatata
    14281 atccatagct ttaaaatttt caggattaaa attactagca cgtagcgtac tataatacat
    14341 tgtcggaatc atgataataa atgtagggtg atattgtgca atcatgtcat tcaattcttc
    14401 gccgttaaag taacgttgaa gaataagtgt gccacctgac attaatactg gtaatacagt
    14461 atcgttaaac cctaaaacat ggaacattgg tgttgataca atcgtaatat agtttgaatt
    14521 gaacttatac gtcagctcta agtttgcgcc gttatgaaca aatgattcat atgagaacat
    14581 cacaccttta ggtgatccgg ttgtaccact tgtataaatt aatgctgcaa gatcttgtgg
    14641 ttcaacaggt gttgcttgaa aaggttggtg ataatctgga tttacgattt catcatattg
    14701 cgctacatca atatccatat gcaataagtt ttggtcaata tcggtgagtg aacttaaatg
    14761 tttttcagca tagaagagca gttttaattg tgcatcttcc acaatggctg caatttcttt
    14821 tgggttaagc cgccaattca atggtaaaaa aaccgcacct gttttaaaac aagcaaatag
    14881 taaatctaat attgcaatat catttggcgc aaaaataccg ataacatcgc cttttttaac
    14941 accttgagat gttaaataat gtgccatatt atcagcgcgt gcattgagtt gttggtatgt
    15001 ccaagatgtt tgttttgcgt gatcaataac ggcaggcttg tcatcatcga agtctgaacg
    15061 tgtttttatc caatcgaaat tcattagtat acccccttta gcttcacttt catactttat
    15121 gaattgattg tttaagttgt ccccattttt ctttgtaaat gctggtatca attaatttta
    15181 aatgatcagc aataattggt ttaaaagcca tttgattcaa aatatcttta tgcaaatcaa
    15241 gacctggagc aatttcaatt agtttcaagc cttgattggt gagctcgaat actgcacgat
    15301 cagtaacaaa atagacttct tgctcgagtg attgtgaata ttgtgcatta aagtcgatat
    15361 ggctcacatc tgatacaaat ttctggtttt gtccttcagt ttcaatgttt aatcgttgat
    15421 tatgacatga gacatgacta ccggctacaa aagtacctga aaagataatt ttatttacag
    15481 attgcgtaat gtctataaag ccgccacatc catttagtcg gtcattgaag tatgacacgt
    15541 tgacattgcc gtgttgatca acttcagcaa agctaagata ggcaactgat acaccattgt
    15601 tataaataaa atcccatgct cgatcatgag gcatgcgcac atctgcattg taattcatac
    15661 caaaatgttc acgactccca acgaatccac cgaaaatgcc aacatctaaa atcggttgca
    15721 catcatgttc aacacattct tcatgcaata aattagagag ttcattattg atgccataac
    15781 cgatgctaat tgtatcgcca taagttaaaa actgagcagc acgtcggaga atcaatttgc
    15841 gactattaaa aggtaatgcg ggttcaggta ttccatcaat tcgttcttct ccagacaagg
    15901 ctggtaaata atgactctga attacttgac ggtgattctt ttcatcttct gtgacgtata
    15961 cataatcgac aagatttcct gggataacaa cttcattcgg ctttagttga tagtcgtcaa
    16021 ctaaagcttt aacttgtaca ataactttcc catgattggc tttcgtgttt aatgcgacat
    16081 gataacactc gctcaagtac gcttcttgag ttaaataaat gttaccttgt tgatctgcgt
    16141 atgttcctct cagtagtgcc acatcaacgc tagggaatgt gtagtgtaag tatgtttcat
    16201 cgttgatggt tactaatgaa actaaatcat cagttgttcg tgtatttact ttaccgccac
    16261 cgtatctagg atcaacagct gtgtttaatc cgattttagt aataactcca ggtaataatt
    16321 gattactctg acgataatga gttgcaatga taccttgtgg taaaaaataa gcttcaatgt
    16381 cattattttt cattgcttgt gccgttttgg aagaagccgt taaaatactc ataatgacac
    16441 gtttaatcat gcgacgttct ataaaatcat ctaaatccgg tgcggcacct aaactatgga
    16501 tatcattcgc taatataaac gttaaatcat tgggcgtatg atatgtgtca tgttgcgcta
    16561 acacagcacg tagaacttcg gcgggtaagt tggctacagc taatgctggt aaaccaatca
    16621 catcaccatc tttaatgata tgttgtaagt cgtgccatgt gatttgtttc aagcaagtca
    16681 cctccatcac atttgataaa atatagcgtt tttacacttt gtgtaaaccc ttacaagaaa
    16741 tataacataa cgacatttaa aatcaattag aaatatcttt ttattctgat aatagacaca
    16801 gtatagacac attttgatgg ttgataacaa ttgtaatatc aagggtttgt aatgaattga
    16861 atatcattaa aatacttata taaaaatatt gctcggaata taaaaaacaa cccatattta
    16921 tttaaaaaat gcaaagacta attatcattt tgatgcatca gtaaggtgag gatgatcagc
    16981 gtacaatcag tggtgtataa tgtaactgat gaaatcattg gagagttgaa ggagatgttg
    17041 aacatatgag agtcaatgtt ttacaacata caccaaatga aggtgttgga tacataggtg
    17101 agtgggcgaa ggaaagcggg cacgaagtct atatttatca cccatatatg aatgatggcg
    17161 tgctacctct tgcggaagaa acagatatgt tagtgatact tggtgggcca atgaatccag
    17221 acgatgagat gccttggatt caatcagagc gcgaattaat tttagtattg atggcacaaa
    17281 acaaaccaat ttatggtgct tgctttggtg cacaacaaat ttcgaaagca ttaggttatg
    17341 cggtgttaaa atcaccagta aaggaagtag gttgggatga tgtttactta gaatcagaaa
    17401 tcattccgaa tataccaagt cgattgaagg cactacattg gcatgaggat atgtttcaaa
    17461 taccgcaaca agcagaatta ttattttcag ggaatcatat tagaaaccaa ggatttatca
    17521 tcgatcatca tgtgattgga ttacaatttc attttgagcc tggtccagac gatgtcagag
    17581 aaatagtact aaatgatgcc caatacatca atcattctat tttagagcaa tcggcggagg
    17641 acatattagc ctttgaggtg cctaaacaga ataggcaggc cattttccga atacttgatt
    17701 ttattactca agcgtaagat gtgagttgta ttttaaaatg agtaaaaaaa cgaatgtaca
    17761 caatcgaaca aagtagttat ataaatatat ggaaaatcca tcaatattaa attaaaatag
    17821 ttttattatg aaaagtgaaa gtaggtaagt ctatggaagg tcttaatcat cgaagaaata
    17881 cagaaaaaga agagacaaca caaacgcaat cagttgcacc taatacaggt gaagagggga
    17941 tgtcatcagc aagtacacaa tcaactaaga cgtccgacat acataatgaa tctatcgata
    18001 aacaaatgga agccaaagcg catgaaacag cgcaaaatac agatttaaaa aacgaagcaa
    18061 gaagtttatt tgataatgca accaaatcaa tcggtagact agccggcaat gatgaaagct
    18121 taaatcttaa tttaaaagat atgttttcag aagtatttaa gccacatact aaaaacgaag
    18181 cagatgaaat atttatagcg ggtactgcta aaactacgcc agcaatttgt gacatatcag
    18241 aagaatgggg gaagccatgg ctcttttctc gagtattcat cgctttcaca gtaacattta
    18301 ttggattatg ggtcatggca gcgattttta ataacactaa cgcgattccg ggtctcattt
    18361 ttattggggc tttaacagta ccattatcgg gtttgttctt cttttatgaa tcaaatgcgt
    18421 ttaaaaatat tagcattttt gaagttatta tcatgttctt tattggcgga gtattttcat
    18481 tactaagtac gatggtatta tatagatttg tcgtttttag tgatcaattc gaaaggtttg
    18541 gttctttaac atttttcgat gcatttttag taggattagt tgaagaaact ggaaaagcac
    18601 tcattattgt ttatttcgtc aataaattga aaacaaataa gattttgaat ggattattaa
    18661 tcggtgctgc tattggtgca gggttcgcag tttttgaatc agcaggttat attttgaatt
    18721 tcgctttagg ggaaaatgtc ccattattag atattgtctt cacacgtgcg tggactgcga
    18781 ttggtggcca tttagtttgg tcagcgattg ttggtgctgc aatagttatt gcgaaagaac
    18841 agcatggctt tgaattcaaa gatatttttg ataaacgctt tttaatattc tttttatcag
    18901 ccgttggttt acatggcatt tgggatacat ctttaactgt acttggcagt gatacgttga
    18961 aaatatttat tttaatcgtt attgtgtgga tacttgtatt cattttaatg ggggcaggtt
    19021 taaaacaagt taatttactg cagaaagaat ttaaagaaca acagaaaaaa gtagacgaat
    19081 aataattaaa gcttatgttg ctcatatgtt tgtgacataa gctattttta taatttgtct
    19141 ctaaaagagt ggaagtggaa tactttttgg agttaaaaag tgtttcacgt taaacaaata
    19201 gtgacaatta gatttatata aaatgaacat gattcactaa aaatatgtaa taatcatttt
    19261 attgaaattc atcaaacaga aagtaataca atcatataag caaattaaac cacgccataa
    19321 tcatattgaa tgacctcggc gtggttttta tagttgaagc ggggctgaga cataaatcaa
    19381 tgtcccacac tcccttatcg ttcaatcgtt gttcgataat cgattaaata gataccttca
    19441 ggtgttactt tataattttt aaccttagag ttagcagcga ctatttgatc gttgtaagca
    19501 atataactgt ttggtacatc tcgacttgat aatttaataa tatcattaga aatattgtga
    19561 cgttccttaa tatctacagt atgattcaat tgattaatta aatcatcaac gttgctatta
    19621 ttgtagtctc ctttattaat agcaccatct tttttatatg cttgattaaa gaaatagcct
    19681 gtatctccac gaggaatcgt tccgaaacta tacatcgttg catcccaggc agaacggtct
    19741 tttaagtaac cttctatgtc atcaacactt ttaatgtcga tttcaatatt agctttttta
    19801 gcatctgatt gtaatacttg cgcaattttc gatagctctg gacgaccgtc atacgtaatt
    19861 aacttaattt ttaaagggtg ttcttttgta taaccatctt tagctaataa cgcttttgct
    19921 tgttcgatat tttgtttggt taacttaggt tctttaatat atggaatttt atcattaaat
    19981 ggactcgttg caggtttcgc gtaaccttga taaatatgat ctgcaatacc ttgtctatca
    20041 atgatatgat ctaatgcttc acgaacggat ttagtcattt ttttattagt atgattatac
    20101 ataagtaaag aagttctaaa tccagattct tttgacactt ttaaattttg attattttct
    20161 atgtcttgaa ctttattaac tgggacatca gttattaaat catctttttg agattctaaa
    20221 tttctgacgc gattattgcc gtcttcttgg tacgtcacag taatatgatc aagtttcggt
    20281 ttaccttgcc aatagtcctt aaaattcgac aatgatattt ttcgagattg cttataatct
    20341 tttatttggt aagggcctgt accaacagga gtttgattaa catctgattt agcatctgta
    20401 tcataaattg ccataaaagg attagctaat tcagatacaa gttcagggta agcgtagttg
    20461 gttttaattg tcagtttttg acctttagcg gtaattgatg atattggtaa tgaatatttg
    20521 accaagtcgc tttttttcat gctattttca aggctagatt tcactttttc tgcagtcaat
    20581 ttttgaccgt tttgaaattt aatattatct tttaattcta tatctaacgt tttatcattt
    20641 ggttgatgat acgatttcac taatactttt tctatttttc cttgatcatt tgttttaaat
    20701 aatgattctg cagcaccaat cttaactggt acatctgttt cataaggtgc aatagacttt
    20761 gtttttaacg gtaacgaaat attcaagtct ttgccagatg aatgcattga gccacatcct
    20821 gataacacta atactgctga aaatatagtt gctagtcttt taaacttcat ttcattaaca
    20881 ctctctttct aattactatg taaaacccaa taattaatat tttaaaactt tattttgtta
    20941 gagtaaaatg ttgttcaagt ttagtgatta ttaaagttca attaattgta gtaattatgc
    21001 tttttaaaaa taaatattag aaatgaagtt agcgacatgt atagtgattc aagacaaaca
    21061 tatataccta agtattgagc aactgctgta gtactacagc ttggttatgt ttagtatctt
    21121 ttgctgaata taacaataga acatgattat gctgatttac aatatccttt aatttttcaa
    21181 aagcctcttt ttgcacatcc tgatcacgta attctttttc atatttttct ttaaaagctc
    21241 caaaaagttt aggatcatgt tggaaccatt gtcgcaactc agtagaaggg gcaatgtctt
    21301 ttaaccaata atctaggtta gcagttcttt tcgaaatacc tctcggccag actctatcga
    21361 ctaggatacg aatagcgtcg gtattatctt tattgtcata aatccgtcca atatctacgg
    21421 tcatcttgtg aactcctttc ttatgaaatt cagtgagcat acatcaatgc atgttgtggt
    21481 gggacgacga aataaatttt gcgaaaatat cattactgtc ccactcccaa ttaaaaaaca
    21541 gccatgacaa agtaaagtca tagctgtttt tgtatatatg ttatttattt ttacgtttag
    21601 ttaaatactt caaaccaact gcaaagacgg tgtaaccggc aagtgtttga atcaatgttt
    21661 tgattaaatt gttatttttg acaacaatat ttgcagtaac aatacttacg aaatataatg
    21721 caaatacttt aacgtaacgt tgattaagtt tcatatgagt actaaacctc ttttctcagt
    21781 tatactgcaa cgcttagtct tggaataaat gtttcgtagt gtacgcgatc catatcgtaa
    21841 tttaaagatt taagtgcttc aatcatagat tgtaagaatt ttgtaccacc acagatataa
    21901 atttcaggtt tatttgctaa aaatgctttt aattcttcag cgccaatata gccttgttta
    21961 tcttttaagt gtgtatataa tttagcgttg tcatgatggc ttgcgatact gttgaagttg
    22021 tctttgaaag gtaaatgttg ttcattttca gcaacttgaa ccatctgtgt atctaaacct
    22081 ttggcagagg cagcttcata catagctact aaaggtgtaa caccaattcc tgaacctaag
    22141 aaaagttgtg gttcagtcgt attctctaat acgaatccac ctacaggcgc agctagatta
    22201 atcatatcgc cttctttaat ctcgtcgtgt aatattgttg aaacttcgcc ttcatgttct
    22261 gttgtgacat cacgtttaac gccaaaagtt aaatggtttt tatcacctga tacgatagaa
    22321 tagtgacgtt tagctctata tggaagttta tcactagaaa catcaactgt gatgtattgg
    22381 cctggtgtaa attcactaaa gtcatattct tcagtttcaa ctgtaaatga tttaatgtct
    22441 tcagattctt gtttaatatt ggtaattttg aaaggtttaa aaccaatcca catcatttga
    22501 tcataaattt ctttttcaat ttggatgaac acatccgcaa tgacgccata tgctttcgcc
    22561 caagcttgaa tgacagggtc gttttcttct aatcctgtca cgtcttgaat ggcttttaat
    22621 aaattttccc ccacaattgg ataatgctca gcataaactt gtaatgcaca gtgtttatat
    22681 gcgactggca taatgactgg cttaataaca cttaagttat cgatattaac cgctgcggcc
    22741 attacagctt gtgctaatgc tgaagattgc atgcctcgtt tttggttcgt ttgattaaac
    22801 atgtttaaaa gttcaggatg cgctttaaac atttttggat aaaagattga cgtaatttct
    22861 gttcctttct ctttaagtaa aggcaccgtt tgtttgataa tgtctttctc ttgttctgta
    22921 agcatgatac tcctccttta atctgtctac tttgattatt ctactaaaaa tttcgatatt
    22981 caattaattg gtttgagaaa atataaataa aatggcaaat ttgataattg tatgacattt
    23041 ttaatttttt aaatacttat caataagcat tgtgtacaat tgtgtatttg aaccccaacg
    23101 attgcgtgca cttatttgac taattcaaaa aacattgttg ttttcttaga aaaaattaaa
    23161 catgataata aaaatgtgaa agtgtaaaaa atcactggcg aagtacgaag actaaagaca
    23221 tctaagatgt aatcgtatac aaattaaaaa ggtgtaaaaa ttaaaataaa atgtgaaata
    23281 aatcacaatt taatattgac ccagtactta atgcgtgtta cattttatat gtgaaataaa
    23341 tcacaaactt aaaagcggat gacacatgac cttttaagtt atgcgttgaa aataaaagag
    23401 atgtttattt gcttttgtat cgtcaataag cagcattaaa ctaacatatt tgaagctaca
    23461 cgtatgttag tgaattaatc ataagggagt ttttgtaatg aacaaattta aagggaacaa
    23521 agttgtatta ataggtaatg gtgcagtagg ttcaagctac gcattttcat tagtgaacca
    23581 aagcattgtt gatgaattag tcatcattga tttagacact gaaaaagttc gaggagatgt
    23641 tatggattta aaacatgcca caccatattc tccaacaaca gttcgtgtga aagctggcga
    23701 atacagtgat tgtcatgatg cggatctagt tgtcatctgt gctggtgctg cacaaaaacc
    23761 tggagaaaca cgtttagatt tagtatctaa aaacttgaaa atatttaaat caattgttgg
    23821 tgaagtaatg gcatcaaaat ttgatggtat tttcttggta gctacaaatc ctgttgatat
    23881 tttagcgtat gcaacatgga aattctctgg tttacctaaa gaacgtgtta taggttctgg
    23941 tacaatttta gactctgcac gctttagatt attgttaagc gaagcgttcg atgttgcgcc
    24001 acgtagtgtc gatgctcaaa ttattggtga acatggtgac actgaattac cagtatggtc
    24061 acacgctaat attgcgggtc aacctttgaa gacattactt gaacaacgtc ctgagggcaa
    24121 agcgcaaatt gaacaaattt ttgttcaaac acgtgatgca gcatatgaca ttattcaagc
    24181 taaaggtgcc acttattatg gtgttgcaat gggattagct agaattactg aagcgatttt
    24241 cagaaatgaa gatgccgtat tgactgtatc agcattatta gaaggcgaat atgatgaaga
    24301 agatgtttac attggtgttc cagcagtcat caatagaaac ggtattcgca acgtcgtaga
    24361 aatcccatta aacgacgaag aacaaagcaa gttcgcacat tcagctaaaa cattaaaaga
    24421 tattatggct gaatcagaag aacttaaata actttttata aaatctatac cattccaaaa
    24481 attgtaaaac cttaccccaa aaaattgtat aaagggctat ttgatacaac tatatgtgtc
    24541 gagtagccct atttttaatg tagtgaaagt cgttgttgaa attaaattca aatctggcac
    24601 ttaagcgtta atcatagcat taagatgatt gtctagcaga ggcgatttgc gggctcacta
    24661 cagtgcatga tgaacttaat gcttcaaatg taacattaaa aataaaagca acgatgtcac
    24721 ttcttacttc gtacatcgtt gccattagtt ctgttgatat ttcggttagt cttaatcccc
    24781 gagcaattct tcaatttcat ttttgataac tgtaacgtga ggcccataaa ttacttgcac
    24841 accagtgcct tgctggatta cacctttggc accagtactt tcgagtaata ctttatcgac
    24901 tttgtcattt tgatgaagtg tgacgcgtag tctcgttgca caacagtcaa cgatttcaat
    24961 gttatcttgg cctcccaaac cagcaacaat agtttgtgct ctttcagtag cctcaacttg
    25021 ttgtgctgca gctttatctt ctcgaccagg tgttttgaaa ttaaatttcg taattaagaa
    25081 tctgaaaacg atgtaataca aacagaacca cacaattcca ataggtatga cgtataggta
    25141 gtttgtttta ctattacctt gtagcacacc aaaaagtaag aaatcgataa agcctccact
    25201 gaaggtttga ccaattgtaa tgttgaaaat gtctgccatc ataaatgcta atccatcaaa
    25261 gaaggcatgg attacataaa gaataggtgc gacaaacaag aaactaaact ctaaaggttc
    25321 ggtaatacct gttaaaaatg aagtgagtgc agcggataac attaaaccgc cgacaacttt
    25381 tttatgttca ggtttagctg tgtgataaat tgcaagtgcg gcaccacata agccgaacat
    25441 catcgtaata aaacggcctg acataaagcg tgacacacct gaataatact tcgtcacatc
    25501 tggatcaccg agttgagcaa agaagatgtt ctgcgtacct tgaactaagt gccctttgac
    25561 ttctaaagta ccaccaagtg ccgtctgcca aaacggtaag taaaaaatat gatgtaaacc
    25621 gagtggacct aacaatctta agatgaagcc ataaacaaaa gtaccgatgg cacctgtttt
    25681 cgttacaaat ccaccaacat gataaatgcc ggcttgtatg cttggccaaa tgaaaaacat
    25741 caatacacct aaaaagattg cggcaaatgc tgtgacaata gggacaaatc tagagccacc
    25801 aaagaaacct aaatacggtg gtaataccac tttgtgatat ttgttgtgaa gtattgcggt
    25861 cataatacct gtggtaatac cgccaaaaac accggtttca accgtttgta taccgagcac
    25921 catgccttgt ccattttgtg caagctgatc ttttgccaat gtgcccgtga tagttaataa
    25981 gccattcata gttgcgttca taattaagaa accgagcagc gcagctaaac ctgcagtacc
    26041 tttatcgctt ctagataatc cgattgcgac accaattgca aagatgaccg gtaaattttg
    26101 gaaaacaata ctacctgcag ctgacattaa tgtaaaaata ttttgtaata aggtaatatc
    26161 taaaataggg tatgctttaa cggtgtttgg attacttaat gcaccaccga tacccaacaa
    26221 tagacctgca gctggtaaga ttgcgatagg tagcataaag gacttgccga actgctgtgc
    26281 tttttcaaat aaagatttca tcaacatccc tcctaattat tctcaatata gcttttgaga
    26341 aatttaatat caatatatat tctgtgtatg aaaatatttt tcataaaaat tgtttgaatc
    26401 atgtaacaat catataaatt gctgtttata ttgttgtgaa aatgagttga caaaagtcgg
    26461 tatagatatg tagacatcct atttttagcg aggtaggttg ataaggcata tcggataaat
    26521 ttataagcca taaaatagat aaatagtgtt tgtatccaaa aatatgagaa agttaaaact
    26581 atttttcaaa ataaacatga ttcaccacat aaataaatat acataagcta tcgataacaa
    26641 cttaagaaag gtggatatat aaatgaaaag aaagattatt atggattgtg atccaggaca
    26701 cgatgatgca atagcattaa ttttagcggg ggcaattgac agtccactag agatattagc
    26761 tgtaacaaca gtcgcaggta atcaatcagt tgacaagaat acgacaaacg ccttgaacgt
    26821 attggatatt atgggacgtc aagatatagc agtagcgaaa ggtgcggata ggccgttaat
    26881 taaatcagct gcctttgctt ctgaaataca tggggaatct ggattagatg gcccgaaact
    26941 accgtcgaca ccatcacgtc aagcagttgc aatgccagca tcagatatga ttataaacaa
    27001 agtgatgacg agtgatacac ctgtaacaat tgtagcgaca ggtcctctta cgaatgtagc
    27061 aacggcattg attcgtgagc caagaatcgc tgagcatatt gaatctatta ctttgatggg
    27121 tggtggtaca tttggaaatt ggacgcctac agcagaattc aatatttggg ttgatgctga
    27181 agcagcgaag cgcgtttttg aaagtgggat tactataaat gtgtttggtt tagatgtaac
    27241 acatcaagtt ttagccgacg atctcgtgat tgaacgcttt gaaagtatca ataatcctgt
    27301 tgcacagttc gtcgtagaat tattgcaatt ctttaagaag acatacaaga ctcactttaa
    27361 tatggacggt ggtccaatac atgatgcttg tacaattttg tatttgttac aaccagaatt
    27421 gtttacaatg gtacccgtta atatcgacat tgaacatcaa agtccactaa cttatggtac
    27481 tatggctgtc gatttaaatc atgttacagg taagcctgcc aatgcttatt ttgctacagc
    27541 agttgatgtt gaagaagtgt ggaacttgat agattataag ttacgtactt acgaataata
    27601 atacttaatt aaatagatac agttagccct aaggcgcctg atataagcgt ccttagggtt
    27661 tttgtattgg attacaatgg tcatgacaat ttgataatga ttgagatatg tatgttaaaa
    27721 atgcttcaaa aaaacacaaa aatatatgta aaaatttcaa ttgctttatt ttaaaagatt
    27781 aaaatcacaa agaataccga tataattgga atgttgtcga aacatcacaa tgatgtacat
    27841 actcattttg aaaatatgtg ttcaaagtgt gtacatcggt gtgacgattg atgataaatt
    27901 gagctcagat gctcaaagga ggatcgaaca tgaacgggga taatcagcaa atactcagag
    27961 aaattgtatt gaatcctact attcatggta aagaacttga atcgatattt ggtttatctc
    28021 gtagacaact aggatatcgc attcaaaaaa tcaatttgtg gcttgaacaa gagggttatc
    28081 caaaacttga aagaacaagc caaggaaatt ttattgtaag ttctgaaatc atgacgttat
    28141 tcaagcgaga tgtatcggag cagcaaatgt taaacggtaa caatgtcatt tttagcatag
    28201 aaacacgtcg ttattattta atgctcatgc tttttagtaa ggaaaacgca atgtctctaa
    28261 accatttttc aattgattta caagtcagta aaaatactgt cattcacgat ataaatcatg
    28321 tgaaagagca attggaaaat catggtttgt cacttaagta ttctcgaaaa catggttatg
    28381 aaattgttgg tgatgaattt gaagttcgcc gtttcttcat taaattgatt gatcaaaggt
    28441 tgaatcatga tattactaaa agtgaagttt taaaggcgct caacttaaca ttcgaagata
    28501 tcgcatatca aaaagacaaa atcaaacagg tagaacaatt tttgaagagt cgctttatag
    28561 acaaatcact tagttcactg ccttatgtcc tttgtgtgat tcgtagacga attcaaagtg
    28621 gtcatgtgat gaatccatta aatattaatt atcagtattt gagggatacg aaagaatatc
    28681 aagcaacgga gattatgacg caacatgagc cggatttgcc agaagcggaa aagttatatt
    28741 tgacattaca cttactttca acaagtgtgc aatggactga tttgcaagaa tcagatagca
    28801 tatcgaattt aacgatggcc atcgctcaaa tgattcacca ttttgaacaa atcactttta
    28861 ttaacattga agataaggag aaattatcac agcaactctt gttacattta acgcctgctt
    28921 tttataggat taaatataac ttaacggatc gtgatgaatt aatcaatcct ttacaaggaa
    28981 attatcaatc cttatttcat atggtgaaac aatcatgcca atcgttaact gaatatttcg
    29041 gaaaatcgtt gcctgataat gaaatagcat atttaaccat gttgttcgga ggtagtttga
    29101 gacgtcaaga tgaaaacttc gatggcaaga taaaagctat tatcgtgtgt acacaaggca
    29161 cgtcagtatc acaaatgatg ttatacgagt tgcgaaactt atttccagaa attattttct
    29221 tagatgcgat ttcacttaga acatttgaaa attacacatt agattatgac atcgtctttt
    29281 caccaatgtt tgtcctaaca cataaaaaat tatttatcac aaaagtagct ttatctgaaa
    29341 atgagcaacg aaagttacgt aaagaagtga tgaagtacat taataaggaa tcggctgaca
    29401 ttgataagga aataaacaag ttaatggcat taattgaacg cacaacgaca gttaatgaca
    29461 ttacagaact acgtgatggt ttagaagatt ttattgcgaa ttataattca atttcaacca
    29521 ttaatggatc gattgtcaca caaaataaga cattagattt agctgacttg ataccagcaa
    29581 ggcacgtgaa aagaatacat catgttgaaa atattgatga agctattgct aaagcaagtg
    29641 atgtgttagt tgctaatcat tttattgata ttaaatatat tcatgagatg caacaggtat
    29701 ttgatgattc gtatatggtt atcatgcaaa atattgctat tccacatgca tactctgaaa
    29761 agcatgtaca taaaacggcg atgagtatgt tgatattaca agaaccaata tacatgtcag
    29821 atggcacagc aatccatatt attgtaccta ttgctgctgt tgataaagtg acacacttaa
    29881 gagcgttact acaattgaga gatgtggcgc aagacaatga tgcaattaag cgcatcatac
    29941 aaagtcgcaa aaattctgat gtaaatgaga ttttaaaaaa ttattcaaat aaagaagcga
    30001 gggaaaatgg atgggacagc aattagtgca taaagaaaat ataatgctca atttgtcggc
    30061 aactgataaa gaatccgtat tgtcacaaat gtcagatgtg ttatttcaaa atgggttcgt
    30121 gaagtcaacg tttaaagatg cagtcatcga cagagaaaaa gaatttgcta ctggtttacc
    30181 aacgcatcta tgttcggtcg ctataccgca tacagatgtc gaacatatta accatagaac
    30241 gataggtgtg gctgttctag aaaaagaagt gccgtttatt gaaatgggaa cacttgatca
    30301 acagacagaa gtgaaaatcg tttttatgtt agcaatggat aaagtagatg atcaacttaa
    30361 gctgttacaa cagttgatgc aaatttttca aagtgaagaa aaattggagc agattctgcg
    30421 aacgaaagat gaaacaattt tagcaacact aatcaatgat tatttggaat ataactaaaa
    30481 attaattgga ggaattgaaa atgaaacaag tattagtagc atgtggtgca ggtattgcaa
    30541 cgtcaacagt agtaaataat gcaattgagg aaatagcaaa ggaacacaat attaaagtag
    30601 atattaaaca aatcaaaatt acagaagttg gaccttatga agacactgca gatttattag
    30661 ttacaactgc aatgacaaaa aaagaatata aattcccagt tatcaacgca cgtaatttct
    30721 taactggtat tggtattgaa gaaacaaaac aacaaatctt aacagagtta caaaaataac
    30781 ggaattgata tgtaacgtgg ttcaataaca tataaatata tgcaaattgc atataaatga
    30841 ctatcacgac aaatgattgt tgatgacata gaaacgcagt gactgtaatt caaagggact
    30901 gcagtcgaat gtagcaaggg ttgtcgttga aatgactgta acgtcaaatg taatcacaat
    30961 cggaaagtat cgtgacaatg catatataac agggagggtt taaatatgag ttacttcact
    31021 gattttgtaa ggggattttt agatttaggt gcaactgtta ttttaccggt tgtcatattc
    31081 ttgcttggcc tattctttag gcagaaaatt ggagcggcat ttaggtctgg tttaacaata
    31141 ggtgtggctt ttgtagggat tttcttagtc atcgatttat tagttaaaaa tttagggcca
    31201 gcagcacaag cgatggttaa aaatttaggc gtcagtctga atgtgattga tgtaggttgg
    31261 ccagcaacat catctatcgc ttgggcatca tctgtcgcag catttattat tccactcgga
    31321 atcatagtta acgttgtatt gctagtaact aaagtgacaa agacgatgaa tgtagatatt
    31381 tggaattttt ggcattatac gtttacagca gcaatggttt atgccgtatc aggcagtatt
    31441 tggcaagcgt tattagcagc agttattttc caagttatct gtttgaaagt agcagattgg
    31501 acagcaccga tgatgagtga gttctttgat ttaccaggtg tatcgattgc tacaggtgct
    31561 acaggaagta caatttctta tgcaccaggt atttacttag ttaaattgtt acaaaaagta
    31621 cccggtctga ataagttaga tgctgatcct gaaacaattc aaaaacgttt tggcgcattt
    31681 ggagaatcta tctttgtcgg cttaatttta ggtttaggta ttggtgtgtt agcaggttac
    31741 aaacctggag acatcattaa tttaggaatg tcaatggctg cagtaatggt attaatgcct
    31801 agaatggtaa aaatcttaat ggaaggttta atgccagttt cagagtctgc aagaacatgg
    31861 ctaaacaaac gttttggcga acgtgaaatt tatattggat tggatgcggc tgtagcatta
    31921 ggtcatccag cggttatttc gacagcctta attttagtac ctatcactgt tttattagcc
    31981 gttattttac caggaaacca agtactacct tttggtgact tagcaactat accatttgtt
    32041 gtcgcgttta ttgttggtgc agcaagagga aacattattc attctgtcat tgtgggtacg
    32101 attatgattg caatttcact atatattgca acagacgtag cacccatttt cacagatatg
    32161 gcgaaaggta cgaatgtaca aatgccaaaa ggttcatctg aaatttcaag tattgatcaa
    32221 ggtggtaata tcgttaacta tcttatcttt aaactattta gtctattcaa ttaaaaatcg
    32281 aggtgtttgt ggtgaaagct ttagtaaaaa caagagaagg acatggcaac ttagaacttc
    32341 ttgataaaga agttgcaaca ccgctagatg ataaagtaaa gattaaagta cattatgcag
    32401 gaatttgtgg cacagatatt catacttatg aaggtcatta taaagttaat tttccagtga
    32461 cattaggtca tgaattttct ggtgaaatcg ttgaagttgg agcagacgtt aaagatttta
    32521 aagttggtga ccgtgtcact tctgaaacga cattctatat ttgtaatgag tgtgaatact
    32581 gtgaatcaaa agactataat ttatgcaacc atcgaaaagg tattggaaca caagttgatg
    32641 gcgcatttac taattatgtc attgcacgtg aagaaagttt gcatcatatt ccagacgaag
    32701 tatcgtatca gtctgcagct atgacagaac cattagcatg tgcacatcat ggtgtttcta
    32761 agattcaagt caattcaggc gatgtagcag ttgtaatggg acctgggcca atcggattac
    32821 ttgtagcaca agtgttgaaa agtaaaggcg caactgttgt ggtaactgga ttggacaatg
    32881 acaaagtcag attagataaa gcagaagcat tgcacatgga ttatgtagtc aatttacaac
    32941 aaacagactt aaaaacgtat atcaatggca ttacagacgg ttacggtgca gatgttgttg
    33001 ttgaatgttc aggtgcagtt ccagcagcac gacaaggttt ggatatttta cgcaaaaaag
    33061 gtttctacag tcaaataggt atttttaagg atgctgaaat tccatttgat atggaaaaag
    33121 tgattcaaaa agaaataaca gttgttggta gtagaagtca aaagccagca gattgggaac
    33181 cttcattgcg acttatggcg gatggtttag taaatgctga agctttggtg acaaaaatat
    33241 atgatatttc gaaatgggac gaggcgtatc aacatttaaa atcaggcgaa ggtattaaag
    33301 cattacttaa gccgctcgat ttagatgaaa atgaaggaga gaattaatat ggtagaatca
    33361 atgctaactt ttatgcttgg gccattaaga caaatcactg atttttatat ggaacattta
    33421 ctcgtaagta attccattgt cattgcaggt tattttgcga caggtatttt taaaaagaaa
    33481 aaagttgtga attaaatcag agttgaggtg atttacaagt gaaagctttg aaattatatg
    33541 gcgtggaaga tttacggtat gaggataatg ataagccagt cattgaaagt gcgaatgacg
    33601 ttattgttaa agtgcgcgca acgggtatat gtggttcagt cacgtcacga tacaaaaaaa
    33661 tgggcccata cattcaaggt atgccatttg gtcatgaatt ttcaggtgtt gtagatgcca
    33721 ttggaagtga tgttacgcat gttaaggtcg gcgacaaagt gacaggttgt ccagccatac
    33781 cttgttatca atgtgagtac tgtttgaaag gtgaatatgc acgatgtgaa aagttgttcg
    33841 tcattggctc atatgaacct ggatcgttcg cggaatatgt caaattgcca gcgcaaaatg
    33901 ttttaaaggt tccagacaat gttgattaca ttgaagcagc aatggttgag ccatcagccg
    33961 ttgttgcgca tggattttat aaatcgaata tacaacctgg tatgactgtt gcagtaatgg
    34021 ggtgtggcag tataggattg ttagctattc aatgggcacg aatatttggt gctgcacata
    34081 tcatcgctat agatatagat gcgcataaac tagatattgc tacatcattg ggcgcacatc
    34141 aaacaatcaa ttcaaaagaa gaagatcttg agaaattcat cgaaaatcat tacgccaatc
    34201 aaatcgattt agctatagaa tcatcaggtg ctaaagttac gattggtcaa atattgacgc
    34261 tacctaaaaa aggtggcgag gtggtattac tcggaatacc atatgatgat attgagattg
    34321 atcgcgttca ttttgaaaaa attctgcgta acgagttgac agtatgtggc tcttggaact
    34381 gtttgtccag tgattttccg ggcaaagagt ggacggcaac cttacattat atgaagacga
    34441 aagatattaa tgtaaagcct attatttctc attttttacc gttagaaaaa ggcccagaga
    34501 catttgataa attagttaac aagaaagaac gatttgataa agtcatgttt acgatttatt
    34561 agtatgcacc ttcgaggacg aaaacgctgg tatagttata gctatgaaag tgcgaatgcc
    34621 gtctggtcta cagatactat cgaaataatt catcttcgaa tatacgttgc taaatagctg
    34681 gtttacttgt gtgaaatatg cttgtgaatc ggttgttttg cattttgtat acttaaaatg
    34741 agatggcaat at
//
LOCUS       DAHHOJ010000016        34445 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.016, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000016 DAHHOJ010000000
VERSION     DAHHOJ010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 34445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34445
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.016"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            113..2818
                     /gene="acnA"
                     /locus_tag="NBH32_000670"
     CDS             113..2818
                     /gene="acnA"
                     /locus_tag="NBH32_000670"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCD1514298.1"
                     /translation="MAANFKEQSKKHFDLNGQSYTYYDLKAVEEQGITKVSNLPYSIR
                     VLLESLLRQEDDFVITDDHIKALSQFGKDGNEGEVPFKPSRVILQDFTGVPAVVDLAS
                     LRKAMDDVGGDITKINPEVPVDLVIDHSVQVDSYANPEALERNMKLEFERNYERYQFL
                     NWATKAFDNYNAVPPATGIVHQVNLEYLASVVHVRDVDGEKTAFPDTLVGTDSHTTMI
                     NGIGVLGWGVGGIEAEAGMLGQPSYFPIPEVIGVRLVNSLPQGATATDLALRVTQELR
                     KKGVVGKFVEFFGPGVQHLPLADRATIANMAPEYGATCGFFPVDDESLKYMKLTGRSD
                     EHIALVKEYLKQNHMFFDVEKEDPNYTDVIELDLSTVEASLSGPKRPQDLIFLSDMKS
                     SFENSVTAPAGNQGHGLDKSEFDKKAEINFKDGSKATMKTGDIAIAAITSCTNTSNPY
                     VMLGAGLVAKKAVEKGLKVPEYVKTSLAPGSKVVTGYLRDAGLQPYLDDLGFNLVGYG
                     CTTCIGNSGPLLPEIEKAIADEDLLVTSVLSGNRNFEGRIHPLVKANYLASPQLVVAY
                     ALAGTVDIDLQNEPIGKGNDGEDVYLKDIWPSIKEVSDTVDSVVTPELFIEEYNNVYN
                     NNELWNEIDVTDQPLYDFDPNSTYIQNPSFFQGLSKEPGTIVPLNGLRVMGKFGDSVT
                     TDHISPAGAIGKDTPAGKYLQDHQVPIREFNSYGSRRGNHEVMVRGTFANIRIKNQLA
                     PGTEGGFTTYWPTNEVMPIFDAAMKYKEDGTGLVVLAGNDYGMGSSRDWAAKGTNLLG
                     VKTVIAQSYERIHRSNLVMMGVLPLEFKKGESADSLGLDGTEEISVNIDENVQPHDYV
                     KVTAKKHDGDLVEFDAMVRFDSLVEMDYYRHGGILQMVLRNKLAQ"
     gene            2958..4525
                     /locus_tag="NBH32_000671"
     CDS             join(2958..3633,3633..4525)
                     /locus_tag="NBH32_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611580.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1514299.1"
                     /translation="MKTLKGAIFMPIKPKVDIDVLLYMFQEYEKGVSFQYLIDTLQLD
                     INVNTLYPKYKYYKTHGIETLLTQKQYNRYSKELKLKVVNEYLNSNQSTQDLAIKYNI
                     RSSTQVKNWIKMYTVGKEIKNQSPKTGVYIMKARKTTFEERVTIVEYYLNHKQSYREV
                     AEHFNISYGQIYQWVQKYQAHGKNGLVDGRGKGKPKSMMTPEEQKEAEIQALKAQNRL
                     LEMENDVFKKVSSIGKRNDSKRKQITAYKTIEALKHQYPIQRLCRILGISRASYYKWV
                     HYQPSELELENEQLKREIESIYHKYNGIYGYRRIYIYIRLKLGKQVNRKRIYCLMKEL
                     NLKAVIRQKRKPYRRSTPQITSENKLNRQFDIDTPNKVWLTDVTEFKIKDGSKIYLSA
                     IYDLGAKRIVSYELGPSNNNQLVFKTFNQAIEKVENTKGILFHSDRGFQYTSKTFKHM
                     LDECGMIQSMSRVSKCIDNGPMEGVWGTIKSEIFRGNKHFKFNSVEEATKTIHDFIHF
                     FNYERITLKMADSV"
     gene            4660..5127
                     /locus_tag="NBH32_000672"
     CDS             4660..5127
                     /locus_tag="NBH32_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1514300.1"
                     /translation="MIYSITEIEARYAETDKMGVIYHGNYATWFEVARLDYISKLGFS
                     YADMEKQGIISPVTDLNVNYKKSIFYPEKVKVKTWVEKYSRLRSVYKYEIFNEKGELA
                     TTGSTELICIKEDTFKPIRLDRYFPDWHEAYSKVQTLNNEGKTVEIMDDIDSL"
     gene            complement(5334..5630)
                     /locus_tag="NBH32_000673"
     CDS             complement(5334..5630)
                     /locus_tag="NBH32_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/YadR/YfhF family protein"
                     /protein_id="HCD1514301.1"
                     /translation="MQIELTDAAVTWFKNELELPENNKVLVFFVRYGGEFQLKQGFSP
                     AFTVEPKEDVDIGYEQQYDDLNVVVAEKDLWYFEDDHIIVNVVDHEDEISYSTK"
     gene            complement(6005..6613)
                     /gene="plsY"
                     /locus_tag="NBH32_000674"
     CDS             complement(6005..6613)
                     /gene="plsY"
                     /locus_tag="NBH32_000674"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCD1514302.1"
                     /translation="MMIIVMLLLSYLIGAFPSGFVIGKLFFKKDIRQFGSGNTGATNS
                     FRVLGRPAGFLVTFLDIFKGFITVFFPLWLPVHADGPISTFFTNGLIVGLCAILGHVY
                     PVYLKFQGGKAVATSAGVVLGVNPILLLILAIIFFVVLKIFKYVSLASIVAAICCVIG
                     SLIIQDYILLVVSFLVSIILIIRHRSNIARIFRGEEPKIKWM"
     gene            6819..8810
                     /gene="parE"
                     /locus_tag="NBH32_000675"
     CDS             6819..8810
                     /gene="parE"
                     /locus_tag="NBH32_000675"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCD1514303.1"
                     /translation="MNKQNNYSDDSIQVLEGLEAVRKRPGMYIGSTDKRGLHHLVYEI
                     VDNSVDEVLNGYGNEIDVTINKDGSISIEDNGRGMPTGIHKSGKPTVEVIFTVLHAGG
                     KFGQGGYKTSGGLHGVGASVVNALSEWLEVEIHRDGNIYHQSFKNGGSPSSGLVKKGK
                     TKKTGTKVTFKPDDTIFKASTSFNFDVLSERLQESAFLLKNLKITLNDLRSGKERQEH
                     HHYEEGIKEFVSYVNEGKEVLHDVATFSGEANGIEVDVAFQYNDQYSESILSFVNNVR
                     TKDGGTHEVGFKIAMTRVFNDYARRINELKTKDKNLDGNDIREGLTAVVSVRIPEELL
                     QFEGQTKSKLGTSEARSAVDSVVADKLPFYLEEKGQLSKSLVKKAIKAQQAREAARKA
                     REDARSGKKNKRKDTLLSGKLTPAQSKNTEKNELYLVEGDSAGGSAKLGRDRKFQAIL
                     PLRGKVINTEKARLEDIFKNEEINTIIHTIGAGVGTDFKIEDSNYNRVIIMTDADTDG
                     AHIQVLLLTFFFKYMKPLVQAGRVFIALPPLYKLEKGKGKTKRVEYAWTDEELNKLQK
                     ELGKGFTLQRYKGLGEMNPEQLWETTMNPETRTLIRVQVEDEVRSSKRVTTLMGDKVQ
                     PRREWIEKHVEFGMQEDQSILDNSEVQVLENDQFDEEEI"
     gene            8810..11212
                     /gene="parC"
                     /locus_tag="NBH32_000676"
     CDS             8810..11212
                     /gene="parC"
                     /locus_tag="NBH32_000676"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCD1514304.1"
                     /translation="MSEIIQDLSLEDVLGDRFGRYSKYIIQERALPDVRDGLKPVQRR
                     ILYAMYSSGNTHDKNFRKSAKTVGDVIGQYHPHGDFSVYEAMVRLSQDWKLRHVLIEM
                     HGNNGSIDNDPPAAMRYTEAKLSLLAEELLRDINKETVSFIPNYDDTTLEPMVLPSRF
                     PNLLVNGSTGISAGYATDIPPHNLAEVIQATLKYIDNPDITVNQLMKYIKGPDFPTGG
                     IIQGIDGIKKAYESGKGRIIVRSKVEEETLRNGRKQLIITEIPYEVNKSSLVKRIDEL
                     RADKKVDGIVEVRDETDRTGLRIAIELKKDVNSESIKNYLYKNSDLQISYNFNMVAIS
                     DGRPKLMGIRQIIDSYLNHQIEVVANRTKFELDNAEKRMHIVEGLIKALSILDKVIEL
                     IRSSKNKRDAKGNLIEVFEFTEEQAEAIVMLQLYRLTNTDIVALEGEHKELEALIKQL
                     RHILDNHDALLNVIKEELNEIKKKFKSERLSLIEAEIEEIKIDKEVMVPSEEVILSMT
                     RHGYIKRTSIRSFNASGVEDIGLKDGDSLLKHQEVNTQDTVLVFTNKGRYLFIPVHKL
                     ADIRWKELGQHVSQIVPIEEDEVVINVFNEKDFNTDAFYVFATQNGMIKKSTVPLFKT
                     TRFNKPLIATKVKENDDLISVMRFERDQLITVITNKGMSLTYNTSELSDTGLRAAGVK
                     SINLKAEDFVVMTEGVSENDTILMATQRGSLKRISFKILQVAKRAQRGITLLKELKKN
                     PHRIVAAHVVTGEHSQYTLYSKSNEEHGLINDIHKSEQYTNGSFIVDTDDFGEVIDMY
                     IS"
     gene            11462..12922
                     /locus_tag="NBH32_000677"
     CDS             11462..12922
                     /locus_tag="NBH32_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD1514305.1"
                     /translation="MKDFDSLIPGWFKEFVHVGTDLIWSQYLIGLLLTAGFFFTISSK
                     FVQLRMLPEMFRALVERPETLEDGKKGISPFQAFAISAGSRVGTGNIAGVATAIVLGG
                     PGAVFWMWVIAFIGAASAFIEATLAQVYKVHDKDGGFRGGPAYYITKGLNQKWLGIVF
                     AILITITFAFVFNTVQSNTIAESLNTQYNISPVITGIILAIVTAIIIFGGVRSIATLS
                     SLIVPIMAIIYIGMVLVILLFNLDQIVPMIGTIIKSAFGIEQVTGGAVGAAVLQGIKR
                     GLFSNEAGMGSAPNAAATAAVPHPVKQGLIQSLGVFFDTMLVCTATAIMILLYSGLKF
                     GDNAPQGVAVTQSALNEHLGSAGGIFLTIAVTLFAFSSVVGNYYYGQSNIEFLSTNRV
                     ILFIFRCLVVVLVFVGAVVKTETVWNTADLFMGLMAIVNIISIIGLSNVAFALMKDYQ
                     KQKKEGKNPVFKPENLEINLFGISAWGANKYKNSDK"
     gene            13422..14273
                     /locus_tag="NBH32_000678"
     CDS             13422..14273
                     /locus_tag="NBH32_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HCD1514306.1"
                     /translation="MGEYIVTKTLNNNVVVCTNNDQEVILIGKGIGFNKKEGMTLNDQ
                     TITIEKIYKLESEQQKAHYKSLVEIADDNVLQVIIDSLNFISNTAMNVDSKQLVVSLT
                     DHIIFAYKRLKQNQVISNPFVMETMQLYSDAYHIAKQVIDQLNAALDVHFPEDEIGFI
                     ALHIASNTEDLSMHEMTLINNVIKKGIDIIESDLVTTVDKESLQYQRFIRHVQFLIRR
                     LRRKEYIHAQDDFVSMIKNHYPICYNTAYKILTMIQKQFDVTISESEIIYLTLHIHHF
                     EERINHS"
     gene            14248..14415
                     /locus_tag="NBH32_000679"
     CDS             14248..14415
                     /locus_tag="NBH32_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514307.1"
                     /translation="MKKGLIIPKLSALYGRFVTVKGICMVSLMLVILHKWTVFIQHLM
                     NILSGGESIIE"
     gene            14408..15616
                     /locus_tag="NBH32_000680"
     CDS             14408..15616
                     /locus_tag="NBH32_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1514308.1"
                     /translation="MNENEKNIRKNFLKFTESRYMKFVGGNDLVFSLIALVLLGIVIF
                     IFEKVSYVFDPFIIVFKTIAAPIIVSLILFYLFNPIVNMMERYRIPRVAGISIIYLAV
                     VGVITLIVNLLIPIIGSQVDSLVKNSPQYLEKLINSIDKIANNTFFSSYYSQINDWLN
                     SLPKKIPSMLSEFTDGFGSKIATFAETIANIGVVIVTTPFVLFFMLKDGHHFKEFSTN
                     IMPPKFRKDFHDLLEKMSVQVGSYIQGQIIVSFCIGILLFIGYSVIGLKYSLVLASIA
                     AVTSVVPYLGPTIAISPAIVIAAITSPWMLLKLAVVWTLVQFVEGHFISPNIMGKTLK
                     IHPLTIIFILLCAGKLLGIVGVILGIPGYAILKVLVTHLFQLFKRRYNRFYGNDVGEY
                     DIKESNKIVE"
     gene            16097..18619
                     /gene="mprF"
                     /locus_tag="NBH32_000681"
     CDS             16097..18619
                     /gene="mprF"
                     /locus_tag="NBH32_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HCD1514309.1"
                     /translation="MNQEVKNKVFSILKITFATALFIFVVITLYRELSGINFKDTLVE
                     FSKINRMSLVLLFIGGGASLVILSMYDVILSRALKMDISLGKVLRVSYIINALNAIVG
                     FGGFIGAGVRAMVYKNYTHDKKKLVHFISLILISMLTGLSLLSLLIVFHVFDASLILD
                     KITWVRWVLYVVSFFLPLFIIYSMVRPPDKNNRFVGLYCTLVSCVEWLAAAVVLYFCG
                     VIVDAHVSFMSFIAIFIIAALSGLVSFIPGGFGAFDLVVLLGFKTLGVPEEKVLLMLL
                     LYRFAYYFVPVIIALILSSFEFGTSAKKYIEGSKYFIPAKDVTSFLMSYQKDIIAKIP
                     SLSLAILVFFTSMIFFVNNLTIVYDALYDGNHLTYYILLAIHTSACLLLLLNVVGIYK
                     QSRRAIIFAMISILLITVATFFTYASYILITWLAIIFVLLIVAFRRARRLKRPVRMRN
                     IVAMLLFSLFILYVNHIFIAGTLYALDIYTIEMHTSVLRYYFWLTILIIAIIIGMIAW
                     LFDYQFSKVRISSKIEDCEEIINQYGGNYLSHLIYSGDKQFFTNENKTAFLMYRYKAS
                     SLVVLGDPLGDENAFDELLEAFYNYAEYLGYDVIFYQVTDQHMPLYHNFGNQFFKLGE
                     EAIIDLTQFSTSGKKRRGFRATLNKFDELNISFEIIEPPFSTEFINELQHVSDLWLDN
                     RQEMHFSVGQFNEEYLSKAPIGVMRNEENEVIAFCSLMPTYFNDAISVDLIRWLPELD
                     LPLMDGLYLHMLLWSKEQGYTKFNMGMATLSNVGQLHYSYLRERLAGRVFEHFNGLYR
                     FQGLRRYKSKYNPNWEPRFLVYRKDNSLWESLSKVMRVIRHK"
     gene            complement(18822..19331)
                     /gene="msrA"
                     /locus_tag="NBH32_000682"
     CDS             complement(18822..19331)
                     /gene="msrA"
                     /locus_tag="NBH32_000682"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1514310.1"
                     /translation="MNINTAYFAGGCFWCMTKPFDTFDGIEKVTSGYMGGHIENPTYE
                     QVKSGTSGHLETVEIQYDVALFSYNKLLEIFFSVIDPLDTGGQYQDRGPQYQTAIFYT
                     NDHQKELAETYIEQLKNTINADKAIATKILPASQFYKAEDYHQDFYKKNPERYAEEQK
                     IRQEYKNKQ"
     gene            19468..20451
                     /locus_tag="NBH32_000683"
     CDS             19468..20451
                     /locus_tag="NBH32_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1514311.1"
                     /translation="MDKETNDNEYRRQSEHRTSAPKRKKKKKIRKLPIILLIVVILLI
                     ALVVYIVHSYNSGVEYAKKHAKDVKVHQFNGPVKNDGKISILVLGADKAQGGQSRTDS
                     IMVVQYDFINKKMKMMSVMRDIYADIPGYGKHKINSAYALGGPELLRKTLDKNLGINP
                     EYYAVVDFTGFEKMIDELMPEGVPINVEKDMSKNIGVSLKKGNHRLNGKELLGYARFR
                     HDPEGDFGRVRRQQQVMQTLKKEMVNFRTVVKLPKVAGILRGYVNTNIPDSGIFQTGL
                     SFGIRGEKDVKSLTVPIKNSYEDVNTNTDGSALQINKNTNKQAIKDFLDED"
     gene            complement(20539..20724)
                     /locus_tag="NBH32_000684"
     CDS             complement(20539..20724)
                     /locus_tag="NBH32_000684"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HCD1514312.1"
                     /translation="MPIVNVKLLEGRSDEQLKNLVSEVTDAVEKTTGANRQAIHVVIE
                     EMKPNHYGVAGVRKSDQ"
     gene            20872..22134
                     /locus_tag="NBH32_000685"
     CDS             20872..22134
                     /locus_tag="NBH32_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HCD1514313.1"
                     /translation="MYNYHLLEDRDVLCIDQKSFFASVSCIEKGLDPLETKLAVVADT
                     KRQGSVVLAATPKLKELGIKTGSRLFEIPHRNDIYIINPSMRKYLNVSVAISKIALRY
                     IPPEDLHQYSIDEFFMDVTDSYHRFSSTVHAFCERLKREIYEETGIYCTVGIGSNMLL
                     SKIAMDVEAKHSQNGIAEWRYQDVPTKLWPIQPLRDFWGINRRTEAKLNKRGIFTIGD
                     LAKYPYKFLKKEFGILGVDMHLHANGIDQSKVREKHKISNPSICKSQILMRDYHFDEA
                     KVVMQELIEDVASRVRARKKVARTIHFAFGYSDEGGVHKQYTLKDPTNLEKDIYKVVM
                     HFADKLCNKQALYRTLSISLSQFINEDERQLSLFEYEYQRKRDECLAKTIDQLHLKYG
                     KGIVSKAVSFTEAGTKHGRLGLMAGHKM"
     gene            complement(22271..23362)
                     /locus_tag="NBH32_000686"
     CDS             complement(22271..23362)
                     /locus_tag="NBH32_000686"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HCD1514314.1"
                     /translation="MTTVLFVGLGLIGGSLASNIKYHNPNTNIIAYDADTSQLDKAKS
                     IGIINEKCLNYSEAIKKADVIIYATPVAITNKYLSKLIDMPTKPGVIVSDTGSTKAMI
                     QQHECNLLKHNIHLVSGHPMAGSHKSGVLNAKKHLFENAYYILVYNEARNEQAANTLK
                     ELLSPTLAKFIVTTAEEHDYVTSVVSHLPHIVASSLVHVSQKNGQEHHLVNKLAAGGF
                     RNITRIASSNAQMWKDITLSNKTYILEMIRQLKSQFQDLERLIESNDSEKLLSFFAEA
                     KSYRDALPAKQLGGLNTAYDLYVDIPDESGMISKVTYILSLHNISISNLRILEVREDI
                     YGALKISFKNPTDRERGMQALSDFDCYIQ"
     gene            23527..24558
                     /locus_tag="NBH32_000687"
     CDS             23527..24558
                     /locus_tag="NBH32_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1514315.1"
                     /translation="MEPILEMIKTLTGINSPSGDTEEAIQFVEKYAKDLGYQTTLTNK
                     GALLITVPGKNDEVQRCITAHVDTLGAMVKEIKEDGRLAIELIGGFTYNAIEGEYCQI
                     KTDAGQIYTGTICLHETSVHVYKNNHEITRDQKHMEIRVDEVTTSEEDTKSLGISVGD
                     FVSFDPRTVITSSGFIKSRHLDDKASVAMILQLLKKLKEEQIILPHTTQFYISNNEEI
                     GYGANASIDSKIKEYIALDMGALGDGQASDEYTVSICAKDASGPYHKQLKSHLVNLCK
                     INNIPYKVDIYPYYGSDASAALHAGADIRHGLFGAGIESSHAMERTHIDSIKATEKLL
                     YAYCLSPIE"
     gene            25050..26456
                     /locus_tag="NBH32_000688"
     CDS             25050..26456
                     /locus_tag="NBH32_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCD1514316.1"
                     /translation="MDIFYKKIKANVTPEVLAQLHSKKIILESTNQQQTKGRYSVVIF
                     DIYGTLTLDNDVLSVSTLKESYQIIERPYHYLTTKINEDYHNIQDEQLKSLPFISGYV
                     GTCSFDLVRHEFPKLQSIQLEDHKQHDVRLYMVEQVYVFDHYKDELYIIATNQFSNST
                     KSDLENRVNKSIEDLTKIQPFMPTQDFDFKTKEIQSNISEERFIEMIQYFKEKITEGD
                     MFQVVPSRIYKYAHHASQHLNQLSFQLYQNLKRQNPSPYMYYLNIDKPYIVGSSPESF
                     VSVKDQIVTTNPIAGTIQRGETTQIDNENMKQLLNDPKECSEHRMLVDLGRNDIHRVS
                     KIGTSKITKLMVIEKYEHVMHIVSEITGKINQNLSPMTVIANLLPTGTVSGAPKLRAI
                     ERIYEQYPHKRGVYSGGVGYINCNHNLDFALAIRTMMIDEQYINVEAGCGVVYDSIPE
                     KELNETKLKAKSLLEVSP"
     gene            26453..27019
                     /locus_tag="NBH32_000689"
     CDS             26453..27019
                     /locus_tag="NBH32_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD1514317.1"
                     /translation="MILVVDNYDSFTYNLVDIVAQHTDVIVQYPDDDNVLNQSVDAVI
                     ISPGPGHPLDDQQLMKIISTYQHKPILGICLGAQALTCYYGGEVIKGDKVMHGKVDTI
                     SITSNTHKVLYQDVPEQFSIMRYHSLISNPDNFPEELKITGRTKDCIQSFEHKERPHY
                     GIQYHPESFATDYGVKIITNFINLVKEG"
     gene            27025..28023
                     /gene="trpD"
                     /locus_tag="NBH32_000690"
     CDS             27025..28023
                     /gene="trpD"
                     /locus_tag="NBH32_000690"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HCD1514318.1"
                     /translation="MTLLTRIKTETILLESDIKELIDILISPSIGTDIKYELLSSYSE
                     REIQQQELTYIVRSLINTMYPHQPCYEGAMCVCGTGGDKSNSFNISTTVAFVVASAGV
                     KVIKHGNKSITSNSGSTDLLNQMNIQTTTVDDTPNQLNEKDLVFIGATESYPIMKYMQ
                     PVRKMIGKPTILNLVGPLINPYHLTYQMVGVFDPTKLKLVAKTIKDLGRKRAIVLHGA
                     NGMDEATLSGDNLIYELTEDGEIKNYTLNATDYGLKHAPNSDFKGGSPEENLAITLNI
                     LNGKDQSSRRDVVLLNAGLSLYVAEKVDTIAEGIELATTLIDNGEALKKYHQMRGE"
     gene            28025..28807
                     /gene="trpC"
                     /locus_tag="NBH32_000691"
     CDS             28025..28807
                     /gene="trpC"
                     /locus_tag="NBH32_000691"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HCD1514319.1"
                     /translation="MTILSEIVKYKQSLLQNGYYQDKLNTLKSVKIQNKKSFINAIEK
                     EPKLAIIAEIKSKSPTVNDLPERDLSQQISDYDQYGANAVSILTDEKYFDGSFERLQA
                     LTTKTTLPVLCKDFIIDPLQIDVAKQAGASMILLIVNILSDKQLKDLYNYAISQNLEV
                     LVEVHDRHELERAYKVNAKLIGVNNRDLKRFVTNVEHTNTILENKKPNHHYISESGIH
                     DASDVRKILHSGIDGLLIGEALMRCDNISEFLPQLKMQKVKS"
     gene            28807..29439
                     /locus_tag="NBH32_000692"
     CDS             28807..29439
                     /locus_tag="NBH32_000692"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HCD1514320.1"
                     /translation="MKLKFCGFTSIKDVTAASQLPIDAIGFIHYEKSKRHQTITQIKK
                     LASAVPNHIDKVCVMVNPDLTTIEHVLSNTSINTIQLHGTESIDFIQEIKKKYSSIKI
                     TKALAADENIIQNINKYKEFVDLFIIDTPSVSYGGTGQTYDWTILKHIKDIPYLIAGG
                     INSENIQKVNQLKLSHQGYDLASGIEVNGRKDIEKMTAIVNIVKGDRENE"
     gene            29432..30646
                     /gene="trpB"
                     /locus_tag="NBH32_000693"
     CDS             29432..30646
                     /gene="trpB"
                     /locus_tag="NBH32_000693"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCD1514321.1"
                     /translation="MNKQIQTEADELGFFGEYGGQYVPETLMPAIIELKKAYKEAKAD
                     PEFQRELEYYLSEYVGRATPLTYAASYTESLGGAKIYLKREDLNHTGAHKINNALGQA
                     LLAKRMGKKKLVAETGAGQHGVASATVAALFDMELVVFMGSEDIKRQQLNVFRMELLG
                     AKVVAVEDGQGTLSDAVNKALQYWVSHVDDTHYLLGSALGPDPFPTIVRDFQSVIGKE
                     IKSQILKKEGRLPDAIVACIGGGSNAIGTFYPFIKDDVALYGVEAAGQGEDTDKHALA
                     IGKGSPGVLHGTKMYLIQDEGGQVQLAHSISAGLDYPGIGPEHSYYHDIGRVTFENAS
                     DTQAMNALINFTKHEGIIPAIESAHALSYVERLAPTMSKEDIIVVTISGRGDKDMETI
                     RQYMAERGLAND"
     gene            30639..31367
                     /locus_tag="NBH32_000694"
     CDS             30639..31367
                     /locus_tag="NBH32_000694"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCD1514322.1"
                     /translation="MTKLFIPYIMGNKDLIENATLLSENGTDIIEIGVPFSDPVADGP
                     VIMEAGQQAIKQGITIDYIFNQLEKHGDQIKCNYVLMTYYNIICHYGEQAFFEKCRDT
                     GVYGLIIPDLPYELSQRLKEQFSHYGVKIISLVAMTTDDKRMKDIVSHAEGFIYTVTM
                     NATTGQNGAFHPELKRKIESIKAIANVPVVAGFGIRTPQHVADIKEVADGIVIGSEIV
                     KRFKSNTREEIIKYLQSIQQTLNN"
     gene            31689..32951
                     /gene="femA"
                     /locus_tag="NBH32_000695"
     CDS             31689..32951
                     /gene="femA"
                     /locus_tag="NBH32_000695"
                     /EC_number="2.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemA"
                     /protein_id="HCD1514323.1"
                     /translation="MKFTNLTAKEFGAFTDSMPYSHFTQTVGHYELKLAEGYETHLVG
                     IKNNNNEVIAACLLTAVPVMKVFKYFYSNRGPVIDYENQELVHFFFNELSKYVKKHRC
                     LYLHIDPYLPYQYLNHDGEITGNAGNDWFFDKMSNLGFEHTGFHKGFDPVLQIRYHSV
                     LDLKDKTADDIIKNMDGLRKRNTKKVKKNGVKVRYLSEEELPIFRSFMEDTSESKAFA
                     DRDDKFYYNRLKYYKDRVLVPLAYINFDEYIKELNEERDILNKDLNKALKDIEKRPEN
                     KKAHNKRDNLQQQLDANEQKIEEGKRLQEEHGNELPISAGFFFINPFEVVYYAGGTSN
                     AFRHFAGSYAVQWEMINYALNHGIDRYNFYGVSGKFTEDAEDAGVVKFKKGYNAEIIE
                     YVGDFIKPINKPVYAAYTALKKVKDRIF"
     gene            32970..34229
                     /gene="femB"
                     /locus_tag="NBH32_000696"
     CDS             32970..34229
                     /gene="femB"
                     /locus_tag="NBH32_000696"
                     /EC_number="2.3.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine glycyltransferase FemB"
                     /protein_id="HCD1514324.1"
                     /translation="MKFTELTVTEFDNFVQNPSLESHYFQVKENIVTRENDGFEVVLL
                     GIKDDNNKVIAASLFSKIPTMGSYVYYSNRGPVMDFSDLGLVDYYLKELDKYLQQHQC
                     LYVKLDPYWLYHLYDKDIVPFEGREKNDALVNLFKSHGYEHHGFTTEYDTSSQVRWMG
                     VLNLEGKTPETLKKTFDSQRKRNINKAINYGVKVRFLERDEFNLFLDLYRETEERAGF
                     VSKTDDYFYNFIDTYGDKVLVPLAYIDLDEYVLKLQQELNDKENRRDQMMAKENKSDK
                     QMKKIAELDKQIDHDQHELLNASELSKTDGPILNLASGVYFANAYEVNYFSGGSSEKY
                     NQFMGPYMMHWFMINYCFDNGYDRYNFYGLSGDFTENSEDYGVYRFKRGFNVQIEELI
                     GDFYKPIHKVKYWLFTTLDKLRKKLKK"
BASE COUNT        12198 a         4872 c         6303 g        11072 t
ORIGIN      
        1 caaaacctaa attaagcgtt ttctaagcat gatgagttgt ttatcaataa atataataat
       61 acaatttaat caaggcatat aaatataaaa atgtatcaag ggggatcatt aaatggctgc
      121 aaattttaaa gagcaatcaa aaaaacattt tgacttgaat ggccaaagtt atacttacta
      181 tgatttaaaa gctgtagaag agcaaggtat tactaaagtt tccaatttac cttattcaat
      241 tcgtgttttg ttagaatctt tacttcgtca agaagatgat tttgtaatta cagacgatca
      301 tattaaagct ttaagtcagt ttggaaaaga tggaaatgaa ggcgaggtac catttaaacc
      361 ttctcgtgtt attttacaag atttcacagg tgtaccagcc gtagttgatt tagcttcttt
      421 acgtaaagca atggatgacg ttgggggaga tattactaaa attaatccag aagtaccggt
      481 ggatttagtt attgaccact cagttcaagt ggatagctat gcaaatccag aagctcttga
      541 acgtaatatg aaattagaat ttgaacgtaa ctatgaacgt tatcagtttt taaattgggc
      601 aacgaaagca tttgataatt acaatgcagt tcctcctgca actggaatag ttcaccaagt
      661 taacttagaa tatttagcaa gtgttgtaca tgttcgtgat gtagatggtg aaaaaactgc
      721 atttccagat acattagttg gtactgattc acatacaaca atgataaatg gtattggcgt
      781 actaggatgg ggtgttggtg gtattgaagc tgaagctgga atgcttggac aaccttctta
      841 tttcccaatt ccagaggtta ttggtgtacg actagtaaat tcattaccac aaggcgcaac
      901 agcaactgat ttagcgttaa gagtaactca agagctacgt aaaaaaggtg ttgttggtaa
      961 atttgtggag ttctttggtc caggtgtaca acatttacca ctagcagacc gtgctacaat
     1021 tgcaaacatg gcaccagagt atggagcaac ttgcggattc ttcccagttg atgatgaatc
     1081 tcttaaatat atgaagttaa ctggtagatc agacgaacat atcgcgctag taaaagaata
     1141 tttgaaacaa aaccatatgt tctttgatgt tgagaaagaa gatcctaatt atacagatgt
     1201 tatcgaattg gatttatcaa cagttgaagc atcgctttca ggaccaaaac gtcctcaaga
     1261 tttaattttc ttaagtgata tgaaatcatc atttgaaaat tctgtaacag ctccagcagg
     1321 caaccaagga cacggtttag ataaaagtga atttgataag aaagctgaaa ttaactttaa
     1381 agatggatca aaagctacaa tgaaaacagg tgatattgca atagcagcaa ttacatcatg
     1441 tacaaataca tctaaccctt atgtaatgtt aggtgcaggt ttagttgcta aaaaagcagt
     1501 tgaaaaaggc ttgaaagttc ctgaatacgt taaaacttct ctagcaccag gatcaaaagt
     1561 tgttaccgga tatttaagag atgctggctt acaaccttat ttagatgatt taggcttcaa
     1621 cttggttggt tatggatgta caacttgtat cggtaattca ggtcctttat taccagaaat
     1681 tgaaaaagcg attgctgatg aggacctatt agtgacatct gtattatctg gtaaccgtaa
     1741 ctttgaaggt cgtatccatc ctcttgttaa agccaattac ctagcttcac cacagttagt
     1801 tgttgcttat gcattagctg gaacggttga tattgattta cagaatgaac ctattggtaa
     1861 aggtaatgac ggtgaagatg tatatttgaa agatatttgg ccatcaatta aagaagtttc
     1921 agataccgtt gatagtgttg taacacctga attatttatt gaagaatata ataacgtata
     1981 caataacaac gaattatgga atgagattga tgtaactgat caacctctat atgactttga
     2041 tcctaattca acatacattc aaaatccatc attcttccaa ggattatcta aagaaccggg
     2101 tacgattgtt ccattaaatg gtttacgtgt tatgggtaaa ttcggtgatt ctgtgacaac
     2161 tgaccacatc tctccagcag gtgcaattgg taaagatacg ccagctggta aatatttaca
     2221 agatcaccaa gtgcctattc gtgaatttaa ttcttatggt tcaagacgtg gtaatcacga
     2281 agtaatggtt cgaggtacgt ttgctaatat acgtattaaa aaccaattag cgccaggcac
     2341 tgaaggtggt tttacaactt attggccaac aaatgaagta atgcctatct ttgatgctgc
     2401 aatgaaatat aaagaagatg gtacaggttt agttgtatta gccggtaacg attatggtat
     2461 gggttcatct cgtgactggg cagcaaaagg tacaaactta ttaggtgtta aaacagttat
     2521 tgcacaaagt tatgaacgta tccatcgttc aaatttagtt atgatgggtg tattaccatt
     2581 agagtttaaa aaaggtgaat cagctgattc tcttggtcta gatggtacag aagaaatttc
     2641 tgttaatatt gatgaaaatg ttcaaccaca tgactacgtc aaagttactg ctaagaagca
     2701 tgatggtgat ttggtagaat ttgacgctat ggttcgtttt gactcacttg ttgaaatgga
     2761 ttactatcgt cacggtggta ttttacaaat ggttttaaga aataaattag cgcaataaaa
     2821 atagatatca cagcaaaatt ttaatcggta tttgaggcac tttgatagtg ctcaaaaata
     2881 cttctataaa ctttatgata taataggtcg gttcaaaacc tttgtgaaat gctcccttca
     2941 aagtagacat tgaaaaaatg aaaactttga agggagctat atttatgcca ataaaaccta
     3001 aagtagacat agatgtactg ctatatatgt ttcaagaata tgaaaaaggc gtgtcgtttc
     3061 aatacttaat cgatacatta caattagata ttaatgtcaa tactttgtat cctaagtata
     3121 aatattataa aacccatggt attgaaacac tacttacaca aaaacaatac aatcgctatt
     3181 caaaggaact aaaattaaaa gttgtaaatg aatatttgaa ttcaaatcaa tcaacacaag
     3241 atctcgccat taaatacaat attcgtagtt cgacacaagt caaaaattgg ataaaaatgt
     3301 atactgtagg aaaagagatt aagaatcaat ctccgaaaac tggggtgtat atcatgaaag
     3361 caagaaaaac cacatttgaa gaacgtgtca caattgttga atattacttg aatcataaac
     3421 aatcttatag agaagttgca gaacacttta atatctcata cggtcaaatt taccagtggg
     3481 tccaaaagta tcaagcccat ggtaaaaatg ggttagtaga tggcaggggc aaaggaaaac
     3541 ccaaatctat gatgacgcca gaagaacaaa aggaagctga aattcaagca ttaaaagcac
     3601 aaaatcgatt attagaaatg gaaaatgacg ttttaaaaaa gtttcaagca ttggaaagag
     3661 aaatgattca aagagaaaac aaatcacggc atacaaaacg atcgaagcgt taaagcatca
     3721 ataccctatc caacgcttgt gtcgtatttt aggcatttct agagcgagtt attataagtg
     3781 ggtacattat caaccgagtg aacttgaatt agagaatgaa cagcttaagc gagaaataga
     3841 atcaatctat cataagtata atggtattta tggctatcgt cgcatttata tctatattcg
     3901 attaaaacta ggtaaacaag taaaccgaaa gcgaatttat tgtttgatga aagaacttaa
     3961 tttaaaagcg gtaatacgtc aaaagcgaaa accttatcga cgttcaacgc cacaaatcac
     4021 atcagaaaat aagcttaatc gacaatttga cattgatacg ccaaataaag tttggttaac
     4081 agatgtaaca gaatttaaaa tcaaagatgg tagtaagata tatttgagtg ccatttatga
     4141 cttaggtgct aaacgtattg ttagttatga gctaggccct tctaacaata atcaattagt
     4201 atttaaaaca ttcaatcaag cgatagaaaa agtagaaaat accaaaggta ttttatttca
     4261 tagtgaccga ggttttcaat acacatcgaa aacatttaaa catatgttgg atgaatgtgg
     4321 catgattcaa agtatgtcac gtgttagcaa atgtatcgat aatggtccta tggaaggtgt
     4381 gtggggtaca ataaaaagtg aaatttttag aggaaataag cactttaaat ttaatagtgt
     4441 agaagaagct acaaaaacaa ttcatgattt tatccatttc tttaattatg aaagaattac
     4501 attaaaaatg gctgattctg tataacaaca aaaccaacca ttaattttaa ttatttaatt
     4561 gtctactaga ctgggtgcag ttcattgtgt tcttggccgg cctcttcttg tattttatgt
     4621 tagaatgaga tataaagtta aactgaaaga aggcttaaaa tgatatatag tattacagaa
     4681 atagaagcgc gttatgctga aactgataag atgggtgtaa tttatcacgg gaattatgca
     4741 acttggtttg aagttgcgcg gttggattat atatcgaagt taggttttag ttatgctgat
     4801 atggaaaaac aaggaatcat ttcacccgtg actgacctca atgtcaatta taaaaagtct
     4861 attttttatc cagaaaaagt taaagttaaa acttgggttg aaaaatattc gagattacgt
     4921 tcagtgtata aatatgagat ttttaatgaa aaaggtgaac ttgcaactac aggttccaca
     4981 gaattgattt gcattaaaga agatactttt aagcctatac ggttggatcg ttatttccca
     5041 gattggcatg aagcctatag taaagtgcaa acgctcaata atgaagggaa aacagtagag
     5101 ataatggatg atattgattc tttataagtt actataaatg tttggagagg caatattaac
     5161 aattaatcta tgttaaatag tcaatacgtt agcattaacg caattagcgt gaagataagc
     5221 taatatatag caaaaacgaa cttgttcata caacatcaga tgtgataaac caaagtgcaa
     5281 cttccgaggt tcagtacaca tcagaatgtg taaacaagtt cgttttaatt tagttatttt
     5341 gtggaataag aaatttcatc ttcgtgatca actacattta caataatgtg gtcatcttca
     5401 aagtaccaca aatctttttc cgctacgaca acatttaaat cgtcatattg ttgttcatag
     5461 ccaatatcaa catcttcctt tggttcaact gtaaaagcag gactaaatcc ttgcttgagt
     5521 tggaattcgc caccatatct tacaaaaaac acgagcactt tattattttc aggtaactca
     5581 agttcatttt taaaccaagt tactgctgca tcagtaagtt ctatttgcat aacaatgacc
     5641 tcctatattt attacagaat ggtagtgggc caactcgtat tgtttgtgga atttcttttc
     5701 gaaattctct gtgttggagc ccaccccaac ctgcacatta ttgtaagctg aatttccata
     5761 agcttctatg ttgggacccc cgaccagaat tgaaaaaacc tagttataag cgcatttcgt
     5821 tctgtcaact tcagctaata taacattgtg gagcctatga cattgattta tgtctcggtc
     5881 tcaaatgttc ttgtaaaaaa ctaagtatag ttggatttta agttaaaaaa attgtttttg
     5941 caaaaataaa ataatccatc aactgcaaaa aaatatgcaa tgatggacta atgtcaaaaa
     6001 attgttacat ccattttatt ttaggttctt cgcctctaaa aatccttgcg atattagagc
     6061 gatgtctaat tatcaatatg attgaaacta agaaactaac gactaataaa atatagtctt
     6121 gaatgataag cgagccaatc acacagcaaa ttgctgcaac gatacttgct aaagaaacat
     6181 atttaaaaat cttcaataca acaaagaaga taattgcaag tattagtaaa agtatcggat
     6241 tgactcccaa gacgacacct gcactagttg caacagcttt gccaccttgg aattttaaat
     6301 aaacaggata aacatgtcca agtatagcgc ataagccaac aattaaacca tttgtaaaaa
     6361 aagtactaat agggccatct gcgtgaactg gtaaccataa agggaagaaa acagttatga
     6421 accctttgaa aatatctaga aatgttacca agaatcctgc aggacgacct aatactctaa
     6481 agctattagt agcgccagta ttaccactac caaattgtct aatatctttt ttgaaaaata
     6541 attttccaat tacgaatcca cttgggaaag cgccgataag ataacttagt agtaacatga
     6601 cgattatcat cataaatatt acacatcctt taatatctta ggactatttt atcatattcg
     6661 tttaaattac ggctaaaatt tttaaaaacg gggattaata tatggaatta agctatgaaa
     6721 gttaattgat acttgcattt tacgctgatt tatataagaa taactattgt atagttttaa
     6781 aaacgaacgt acgtttgcag gaggcgaaat cattggcaat gaataaacaa aataattatt
     6841 cagatgattc aatacaggtt ttagaggggt tagaagcagt tcgtaaaaga cctggtatgt
     6901 atattggatc aactgataaa cggggattac atcatctagt atatgaaatt gtcgataact
     6961 ccgtcgatga agtattgaat ggttacggta acgaaataga tgtaacaatt aataaagatg
     7021 gtagtatttc tatagaagat aatggacgtg gtatgccaac aggtatacat aaatcaggta
     7081 aaccgacagt cgaagttatc tttactgttt tacatgcagg aggtaaattt ggacaaggcg
     7141 gctataaaac ttcaggtggt cttcacggtg ttggtgcttc agttgtaaat gcattgagtg
     7201 aatggcttga agttgaaatc catcgagatg gtaatatata tcatcaaagt tttaaaaacg
     7261 gtggttcgcc atcttctggt ttagtgaaaa aaggtaaaac taagaaaaca ggtaccaaag
     7321 taacatttaa acctgatgac acaattttta aagcatctac atcatttaat tttgatgttt
     7381 taagcgaacg actacaagag tctgcgttct tattgaaaaa tttaaaaata acgcttaatg
     7441 atttacgcag tggtaaagag cgtcaagagc atcaccatta tgaagaagga atcaaagagt
     7501 ttgttagtta tgtcaatgaa ggaaaagaag ttttgcatga cgtggctaca ttttcaggtg
     7561 aagcaaatgg tatagaggta gacgtagctt tccaatataa tgatcaatat tcagaaagta
     7621 ttttaagttt tgtaaataat gtacgtacta aagatggtgg tacacatgaa gttggtttta
     7681 aaatagcaat gacacgtgta tttaatgatt atgcacgtcg tattaatgaa cttaaaacaa
     7741 aagataaaaa cttagatggt aatgatattc gtgaaggttt aacagctgtt gtgtctgtac
     7801 gtattccaga agaattatta caatttgaag gacaaacgaa atctaaattg ggtacttctg
     7861 aagctagaag tgctgttgat tcagttgttg cagacaaatt gccattctat ttagaagaaa
     7921 aaggacaatt gtctaaatca cttgtgaaaa aagcgattaa agcacaacaa gcaagggaag
     7981 ctgcacgtaa agctcgtgaa gatgctcgtt caggtaagaa aaacaagcgt aaagacactt
     8041 tgctatctgg taaattaaca cctgcacaaa gtaaaaacac tgaaaaaaat gaattgtatt
     8101 tagtcgaagg tgattctgcg ggaggttcag caaaacttgg acgagaccgc aaattccaag
     8161 cgatattacc attacgtggt aaggtaatta atacagagaa agcacgtcta gaagatattt
     8221 ttaaaaatga agaaattaat acaattatcc acacaatcgg ggcaggcgtt ggtactgact
     8281 ttaaaattga agatagtaat tataatcgtg taattattat gactgatgct gatactgatg
     8341 gtgcgcatat tcaagtgcta ttgttaacat tcttcttcaa atatatgaaa ccgcttgttc
     8401 aagcaggtcg tgtatttata gctttacctc cactttataa attggaaaaa ggtaaaggca
     8461 aaacaaagcg agttgaatac gcttggacag acgaagagct taataaattg caaaaagaac
     8521 ttggtaaggg cttcacgtta caacgttaca aaggtttggg tgagatgaac cctgaacaat
     8581 tatgggaaac gacgatgaac ccagaaacac gaactttaat tcgtgtacaa gttgaagatg
     8641 aagtgcgttc atctaaacgt gtaacaacat taatgggtga caaagtacaa cctagacgtg
     8701 aatggattga aaagcacgtt gagtttggta tgcaagagga ccaaagtatt ttagataatt
     8761 ctgaagtaca agtacttgaa aatgatcaat ttgatgagga ggaaatctag tgagtgaaat
     8821 aattcaagat ttatcacttg aagatgtttt aggtgatcgc tttggaagat atagtaaata
     8881 tattattcaa gagcgtgcat tgccagatgt tcgtgatggt ttaaaaccag tacaacgtcg
     8941 tattttatat gcaatgtatt caagtggtaa tacacacgat aaaaatttcc gtaaaagtgc
     9001 gaaaacagtc ggtgatgtta ttggtcaata tcatccacat ggagacttct cagtgtacga
     9061 agcaatggtc cgtttaagtc aagactggaa gttacgacat gtcttaatag aaatgcatgg
     9121 taataatggt agtatcgata atgatccgcc agcggcaatg cgttacactg aagctaagtt
     9181 aagcttacta gctgaagagt tattacgtga tattaataaa gagacagttt ctttcattcc
     9241 aaactatgat gatacgacac tcgaaccaat ggtattgcca tcaagatttc ctaacttact
     9301 agtgaatggt tctacaggta tatctgcagg ttacgcgaca gatataccac cacataattt
     9361 agctgaagtg attcaagcaa cacttaaata tattgataat ccggatatta cagtcaatca
     9421 attaatgaaa tatattaaag gtcctgattt tccaactggt ggtattattc aaggtattga
     9481 tggtattaaa aaagcttatg aatcaggtaa aggtagaatt atagttcgtt ctaaagttga
     9541 agaagaaact ttacgcaatg gacgtaaaca gttaattatt actgaaattc catatgaagt
     9601 gaacaaaagt agtttagtaa aacgtatcga tgaattacgt gctgacaaaa aagtcgatgg
     9661 tatcgttgaa gtacgtgatg aaactgatag aactggttta cgaatagcaa ttgaattgaa
     9721 aaaagatgtg aacagtgaat caatcaaaaa ttatctttat aaaaactctg atttacagat
     9781 ttcatataat ttcaacatgg tcgctattag tgatggtcgt ccaaaattga tgggtattcg
     9841 tcaaattatt gatagttatt tgaatcatca aattgaggtt gttgcaaata gaacgaagtt
     9901 tgaattagat aatgctgaaa aacgaatgca tatcgttgaa ggtttgatta aagcgttgtc
     9961 aattttagat aaagtaatcg aattgattcg tagctctaaa aacaagcgtg acgctaaagg
    10021 aaaccttatc gaagtattcg agttcacaga agaacaggct gaagcaattg taatgttaca
    10081 gttatatcgt ttaacaaaca ctgacatagt tgcgcttgaa ggtgaacata aagaacttga
    10141 agcattaatc aaacaattac gtcatattct tgataaccat gatgcattat tgaatgtcat
    10201 aaaagaagaa ttgaatgaaa ttaaaaagaa attcaaatct gaacgactgt ctttaattga
    10261 agcagaaatt gaagaaatta aaattgacaa agaagttatg gtgcctagtg aagaagttat
    10321 tttaagtatg acacgtcatg gatatattaa acgtacttct attcgtagct ttaatgctag
    10381 cggtgttgaa gatattggtt taaaagatgg tgacagttta cttaaacatc aagaagtaaa
    10441 tacgcaagat accgtactag tatttacaaa taaaggtcgt tatctattta taccggttca
    10501 taaattagca gatattcgtt ggaaagaatt ggggcaacat gtatcacaaa tagttcctat
    10561 cgaagaagat gaagtggtta ttaatgtctt taatgaaaag gactttaata cagatgcatt
    10621 ttatgttttt gcgactcaaa atggcatgat taagaaaagt acagtgcctc tatttaaaac
    10681 aacgcgtttt aataaacctt taattgctac taaagttaaa gaaaatgatg atttgattag
    10741 tgttatgcgc tttgaaagag atcaattaat taccgtcatt actaataaag gtatgtcatt
    10801 aacgtataat acaagtgaac tatcagatac cggattaagg gcagctggtg ttaaatcaat
    10861 aaatcttaaa gctgaagatt tcgttgttat gacagaaggt gtttctgaaa atgatactat
    10921 attgatggcc acacaacgcg gctcgttaaa acgtattagt tttaaaatct tacaagttgc
    10981 taaaagagca caacgtggaa taactttatt aaaagaatta aagaaaaatc cacatcgtat
    11041 tgtagctgca catgtagtga caggtgaaca tagtcaatat acattatatt caaaatcaaa
    11101 tgaagaacat ggtttaatta atgatattca taaatctgaa caatatacaa atggctcatt
    11161 cattgtagat acagatgatt ttggtgaagt aatagacatg tatattagct aaaaactata
    11221 tgcaatcacg aaattaaatg ataaaataca gtaatgttaa attttgacta aattcaaggg
    11281 atttatatta aatgctgacc aagtacttat cgttaaatta gcgatatact ttgaccaaac
    11341 taatataaat ctcttgtatt gtataagaaa tgatgatgta tctctatatt tactgaagtt
    11401 tcaattcggt ctaattttat agaaggtgca tgattcatgt agaggatgcg aggggtttat
    11461 tttgaaagat ttcgatagtt taattcctgg atggtttaaa gaatttgtcc atgttggtac
    11521 cgatttaata tggtctcaat atttaattgg tctattattg acagctggat tcttctttac
    11581 aattagttct aaattcgttc aattacgaat gttacctgaa atgtttagag ctttagtaga
    11641 acgtccagaa actttagaag atggtaagaa gggtatttcg ccattccaag catttgctat
    11701 tagtgctggt tcgagagttg gtactggtaa tattgctggt gttgcgactg cgattgtttt
    11761 aggcggtcca ggtgcagtgt tttggatgtg ggttattgca tttataggtg cagcgagtgc
    11821 atttatagaa gcgactttgg ctcaggttta taaagtacat gataaagatg gtggattccg
    11881 tggtggtcca gcttactata ttactaaagg tttaaatcaa aaatggctag gtatcgtatt
    11941 tgcgatttta attacaatta catttgcatt tgtatttaac acagtgcaat ctaatacaat
    12001 tgcggagtcg ttaaatacgc aatataatat tagtccagta atcacaggta ttattttagc
    12061 aatcgtaaca gctattatta tatttggtgg tgtacgtagt attgctacgt tatcttcgtt
    12121 aattgtaccg attatggcta tcatttacat tggtatggtt ttagtaatat tgctatttaa
    12181 tttagatcaa attgttccta tgataggtac gattattaaa agtgcatttg gtatcgaaca
    12241 agtaactggt ggcgctgtag gtgctgcggt tcttcaaggt atcaaacgtg gtttattctc
    12301 taacgaagct ggtatgggtt ctgcgccgaa tgcagcggca actgctgccg tgccacaccc
    12361 tgttaagcaa ggtttaatcc aatcattagg tgtgttcttt gatacaatgt tggtttgtac
    12421 agcaactgca atcatgattt tactatattc aggattgaaa tttggtgata acgcacctca
    12481 aggtgttgca gttactcaat cagcacttaa tgagcattta ggttctgctg gaggtatttt
    12541 cttaacaata gcagttacac tgtttgcatt ttcatctgtt gtaggtaatt actattacgg
    12601 tcaatctaat attgaatttt tatcaacaaa ccgtgtaata ttatttatct ttagatgtct
    12661 tgttgtagta cttgtctttg tcggtgcagt tgtaaaaaca gaaacagtat ggaatacggc
    12721 agacttattt atgggcttaa tggcaattgt aaacattatt tccattatag gactgtccaa
    12781 tgtagctttt gcattgatga aagattatca aaagcagaaa aaagaaggca agaatcctgt
    12841 ctttaaacct gaaaacttag aaattaactt atttggaatt agtgcttggg gcgctaacaa
    12901 atataagaac tctgataaat aaatattttt aatgtaacga ttcattatat aaaaatcaaa
    12961 taaaccctga ggttttcaaa ttaaagcgaa aatctcaggg ttttaaattt gtactcaata
    13021 atcatctgta tcattacact agttaatcca agatttgatt atatattaat atagtaaggt
    13081 tgagatttat ttcctccatt ggtgtgcaaa gactatacca ttgataagac atattgttgg
    13141 atagatgaat ataaatttat tttagctttg cacaatttaa tagtacgttt tagtttgtat
    13201 tgagtattaa ttcagataat atataaataa atagaattat ttgtaaatat gggagtcaga
    13261 tttttttatt atagttattc cactttgaga ggcatcgaaa ttaaattaag atgtatcata
    13321 taggataggc gtgtaataga acacaagtat ttaatttatt atactttcaa gttaggcatt
    13381 tccgatatat aatcttaata atagaaaaat aggtgatgat aatgggagaa tatattgtta
    13441 ctaaaacatt gaacaacaat gtcgtagtat gtactaataa tgatcaagaa gttattttaa
    13501 tcggtaaagg tattggtttt aacaaaaaag agggaatgac gttaaacgac caaactatta
    13561 caatagagaa aatttataaa ttagagagtg agcaacaaaa agcacattat aaaagtttag
    13621 ttgaaatcgc tgatgataat gtattacaag taattattga ttcgttaaat tttatttcta
    13681 atactgcgat gaatgttgat tcaaaacaac ttgtagtttc attaacggat catattatat
    13741 ttgcatataa acgcttaaaa caaaatcaag ttattagcaa tccatttgtt atggaaacta
    13801 tgcagttata tagtgatgca tatcatattg ctaaacaggt aattgatcag ttaaatgcag
    13861 cattagatgt acattttcct gaagatgaga taggatttat tgcattacat attgcatcta
    13921 acacagaaga tttatctatg catgagatga ctttgatcaa taatgttatt aaaaaaggta
    13981 tagatatcat tgaatcagac cttgtgacaa ctgttgataa ggaatcatta caataccaac
    14041 gttttataag acatgtacaa tttttaattc gccgattaag aagaaaagaa tatatacatg
    14101 cacaagatga ttttgtgtct atgattaaaa atcactatcc gatttgctat aacacagcat
    14161 ataaaatttt aactatgata caaaaacaat ttgatgttac tatcagtgag tctgaaatta
    14221 tatatttaac attacacatt catcattttg aagaaaggat taatcattcc taaattaagt
    14281 gcattatacg ggcgtttcgt tactgtaaag ggtatttgta tggtatcatt gatgttagta
    14341 attttacata agtggactgt ttttatacaa catcttatga atattttaag tggtggtgaa
    14401 tctataattg aatgaaaatg aaaagaatat aagaaagaac tttttaaaat ttaccgaatc
    14461 acggtatatg aagtttgttg gtgggaatga tttagtcttc tcattaatag ctctagtatt
    14521 gttgggtatt gttattttta ttttcgaaaa agtatcatac gtttttgatc cttttatcat
    14581 cgtttttaag acgatagcag cacctatcat cgtctcttta attctattct atctatttaa
    14641 cccaatcgta aatatgatgg aacgttatag aataccaaga gttgcaggta tttctattat
    14701 ttatctagct gtagtaggtg ttattacgtt aattgttaat ttattgatac ctattattgg
    14761 ttcgcaagta gatagtttag ttaaaaattc accgcaatat ctagaaaaat taattaattc
    14821 tattgataaa atagcaaata atacgttttt ctcttcgtat tatagtcaaa ttaatgattg
    14881 gttaaattct ttacctaaga aaataccatc tatgttaagt gaatttacag atggctttgg
    14941 gtctaaaatt gcaacgtttg cagaaacgat tgctaatatt ggcgttgtga ttgtaacaac
    15001 accatttgta ctattcttta tgcttaaaga tggacatcac ttcaaagaat tttcaacgaa
    15061 tattatgcca ccgaaattcc gaaaagattt tcatgatcta cttgaaaaaa tgagtgttca
    15121 agttggttca tacattcaag gacaaattat tgtttcattc tgtatcggta tattgttgtt
    15181 tatcggttat tcggttatcg ggttgaaata tagcttagta ttagctagta ttgcggcagt
    15241 tacaagtgtt gtaccatatt tagggcctac aatagcgatt tcaccagcta ttgtaatagc
    15301 tgctataact tcgccgtgga tgctcttaaa attagcagta gtatggactt tagtacaatt
    15361 tgttgaaggg cacttcattt caccaaatat catgggtaaa acacttaaga ttcatccact
    15421 tacaatcatt ttcattttac tttgtgcagg caaattgctt ggtattgtag gcgttatttt
    15481 aggtattccg ggatatgcta ttttaaaagt attagttact catttattcc aattatttaa
    15541 acgtcgatac aatcgtttct atggtaatga tgtaggtgaa tatgatatta aagaaagtaa
    15601 taaaatagtt gaataagtaa ttaaaaatac caatagacta tacgtttgtt ggtatttttt
    15661 atggtgctta taatgttggg cactcacatt tatgattatc agcacatgtt tagcttataa
    15721 atttttacga aagtttagcc gtttctaaag tacataataa taaaacgagt atttgccact
    15781 tgattagtac ttcattatta tgtcgaaaat aaaaacaagt ggtattttta atatattaag
    15841 aagcactcat aatcagctgt taattaataa tatttttcat aagtattgat tcatcatttt
    15901 ctttatgtta aatatgaatc agtatagata accatattgt tctgtttgag atagaaatat
    15961 tttctattta cttcttaata cagatgtcaa tatgacaaaa gtgttatata aatcaaaggt
    16021 aaatgatatg ctatttttat ggtttatatg atatattttg atttataaca gaaataatta
    16081 gaattgatgt gaaaaaatga atcaggaagt taaaaacaaa gtattttcaa tcttaaaaat
    16141 tacgtttgct acagctttat ttatttttgt agtaatcacg ttgtatcgag agttatctgg
    16201 aattaacttt aaagatactt tggttgaatt tagtaaaatc aaccgtatgt cccttgtctt
    16261 attatttatt ggtggtgggg catcgcttgt tattctctca atgtatgatg tgattttatc
    16321 tagagcttta aaaatggata tttcattagg aaaagtttta agagtaagtt atatcattaa
    16381 tgcattaaat gcgattgtag gctttggtgg ctttattggt gccggtgtaa gagcaatggt
    16441 ttacaaaaac tatacgcatg ataaaaagaa attggttcac tttatttcat taatactgat
    16501 ttcaatgttg acaggtttaa gcttattatc attgctaatt gtattccatg ttttcgatgc
    16561 atctttaatc ttagataaga ttacatgggt aagatgggta ttatatgtag tgtcattttt
    16621 cttaccatta ttcattattt attcaatggt tagaccaccc gataaaaaca atcgttttgt
    16681 aggattgtac tgcactttag tgtcgtgtgt tgaatggtta gcagctgcag ttgtattata
    16741 tttctgtggt gtaattgttg acgctcatgt atcattcatg tcctttattg caatatttat
    16801 cattgctgca ttatcaggtt tagtcagctt tattcctggt ggtttcggcg ctttcgattt
    16861 agttgtatta ctaggattta aaactttagg tgtccctgag gaaaaagtat tattaatgct
    16921 acttctatat cgttttgcgt actattttgt accggtaatt attgcattaa ttttatcatc
    16981 atttgaattt ggtacatcag ctaagaagta cattgaggga tctaaatact ttattcctgc
    17041 taaagatgtt acgtcatttt taatgtctta tcaaaaggat attattgcta aaattccatc
    17101 attatcatta gcaattttag tattctttac aagtatgatc ttttttgtaa ataacttaac
    17161 gattgtttac gatgctttat atgatggaaa tcacttaacg tattatattc tattggcaat
    17221 tcatactagt gcttgtttat tacttttact gaatgtagtt ggtatttata agcaaagtag
    17281 acgtgccatt atctttgcta tgatttcaat tttattaatc acagtggcga cattcttcac
    17341 ttacgcttca tatattttaa taacatggtt agctattatt tttgttctgc ttattgtagc
    17401 tttccgtaga gcacgtaggt tgaaacgccc agtaagaatg agaaatatag ttgcaatgct
    17461 tttattcagt ttatttattt tatatgttaa ccatatattt attgctggaa cgttatatgc
    17521 attagatatt tatacgattg aaatgcatac atctgtattg cgctattact tctggcttac
    17581 gattttaatc atcgctatca tcataggtat gattgcatgg ttgtttgatt atcaatttag
    17641 caaagtacgt atttcttcta aaattgaaga ttgcgaggag attattaatc agtacggcgg
    17701 taattatttg agtcacttga tatatagtgg tgacaagcag tttttcacta atgaaaataa
    17761 aacagcattt ttaatgtatc gttataaagc aagttcatta gtggttcttg gagatccgtt
    17821 aggtgatgaa aatgcctttg atgaattgtt agaagcattc tataattacg ctgagtattt
    17881 aggctatgat gttatattct atcaagttac agatcaacac atgcctttat atcataattt
    17941 cggtaaccaa tttttcaaat taggtgaaga agcaattatt gatttaacgc aattttcaac
    18001 ttcaggtaaa aaacgccgtg gatttagagc gactttaaat aaattcgatg aacttaatat
    18061 ttcgttcgaa attattgaac caccgttttc aactgaattt ataaatgaac ttcaacatgt
    18121 aagtgattta tggctagata atcgtcagga aatgcatttc tctgttggtc aatttaatga
    18181 agaatactta tctaaagcgc caattggtgt aatgcgaaat gaagaaaatg aagtaattgc
    18241 attctgtagt ttaatgccaa catactttaa tgatgccatt tcagtcgatt taattagatg
    18301 gttgccagag ttagatttac cattaatgga tggattatac ttgcacatgt tactttggag
    18361 taaagaacaa ggttatacaa aatttaatat gggtatggca acgttatcaa acgttggtca
    18421 attgcattat tcatatttaa gagaacgact tgcaggccgt gtctttgaac atttcaacgg
    18481 tctatatcgt ttccaaggat tacgtcgtta taaatctaaa tataatccga attgggaacc
    18541 acgcttttta gtttatcgta aagataattc gctttgggaa tcactttcta aagtaatgcg
    18601 tgtaatacgt cacaaataat taaaatccaa gtgctaagag gtatacagtt atgcctgaag
    18661 cgcttggatt ttttggtttc tataaagaat gatttataaa aaacggtaca gataagaata
    18721 tttaagtttc ctatctgtac cgtattttaa tattgaaatg agcttaagtt aagtcgccta
    18781 acttttatta tcagtccaat cagtttcatc aagtataaat gttattgctt atttttgtat
    18841 tcttggcgta ttttttgttc ttctgcatag cgctctggat ttttcttata aaaatcttgg
    18901 tgatagtctt cggctttgta aaattgtgac gctggtaata tttttgttgc aattgcctta
    18961 tcagcattaa tcgtattttt aagctgctcg atataagtct cagcgagttc tttttgatga
    19021 tcattagtgt agaaaatagc tgtttgatat tgagggccac ggtcttgata ttgaccacct
    19081 gtatctaatg ggtcaatgac tgagaaaaat atttctaata acttattgta tgagaataat
    19141 gcaacatcat attgaatttc aacagtttct aaatgaccac tcgtacctga ttttacttgt
    19201 tcgtaagtag gattttcaat atgtccgccc atatatccag aagttacttt ttctatgccg
    19261 tcaaaggtgt caaatggttt cgtcatacac caaaagcaac ctccggcaaa ataagctgta
    19321 ttaatattca tttttgacat cctttcatta gaccttagta cgatttatta agaaatcact
    19381 tgctttttga attgttttta tataacgtta atatgtgatt attatagtac acaaaaatct
    19441 atttttgaag atataaggta ggtaaatatg gataaagaaa ctaatgacaa cgaatataga
    19501 cgtcaaagtg aacatcgcac ttcggcgcct aagcgaaaaa agaagaagaa aattaggaaa
    19561 ttacctatca ttcttctgat tgttgtaatt ttacttatcg cattagttgt atatattgtg
    19621 catagttaca atagcggtgt agaatatgcc aagaaacatg cgaaagatgt taaagtacat
    19681 caatttaatg gaccagtaaa aaatgatggt aaaatttcta ttcttgtact cggtgcagat
    19741 aaagcacaag gtggacaatc aagaacagat tctatcatgg ttgttcaata tgactttatc
    19801 aataaaaaga tgaaaatgat gtctgtcatg cgtgatattt atgcagatat tccaggatat
    19861 ggaaaacaca aaattaattc agcatacgct ttaggtggtc cagagctact tagaaaaaca
    19921 cttgataaaa atttaggaat taatcctgaa tattatgcag tagttgattt tactggattt
    19981 gagaaaatga ttgatgaatt aatgccagaa ggtgtaccaa ttaatgtcga aaaagatatg
    20041 tcgaaaaata ttggtgtatc tttgaaaaag ggtaaccata ggttgaatgg taaagaatta
    20101 cttggttatg caagattccg tcacgaccct gaaggtgact tcggacgcgt gcgacgtcag
    20161 caacaagtga tgcaaacatt gaaaaaagaa atggttaatt ttagaacagt tgttaaatta
    20221 ccaaaagtcg caggtatttt aagaggctat gtgaatacaa acattcctga ttcagggatt
    20281 ttccaaacag gtttgagttt tggtatccga ggtgaaaaag atgttaagtc attgactgtg
    20341 ccaatcaaga actcatacga agatgtcaat acaaatactg atggtagtgc attacagatt
    20401 aataaaaaca caaataaaca agctattaaa gactttttag atgaagatta aaaataaaca
    20461 aggcgatttc tatcatactc agatagaagt cgccttgttc gttttcaaag aattatttgg
    20521 catgcatctt atgaagaact attgatctga ctttcttacg ccagccacac cataatggtt
    20581 tggtttcatt tcttctataa caacgtgaat tgcttgtcta tttgctcccg ttgttttttc
    20641 tacggcgtca gtaacttcgc taactaaatt ttttaattgt tcatccgaac gaccttctaa
    20701 taattttaca ttgacgattg gcatcatcca aacctcctaa tcattattaa tttgagtata
    20761 tcatgtaata cttaaaaatt catttgcaaa ttaacagaac acttgttcta aaatagttga
    20821 aatagaacac gtgttcgtat ataatagaat tcaagtaagg gaggaataac agtgtataat
    20881 tatcatttat tagaagatag ggatgttcta tgtattgacc aaaaaagttt ttttgcgagt
    20941 gtttcttgta ttgaaaaagg gctagatcca ttagaaacaa agttagctgt tgttgcagat
    21001 actaagcgtc agggttctgt agtattggct gcgacaccta aattaaaaga attaggcatc
    21061 aagacagggt cgcgattgtt tgaaatacca catagaaatg atatttacat tatcaatcca
    21121 agtatgcgta aatatcttaa tgtttcagtt gctatttcta agattgcatt gcgttatatt
    21181 ccacctgaag atttacacca atatagtatt gacgaatttt ttatggatgt tactgatagc
    21241 tatcatagat ttagttctac agtacatgca ttttgcgaaa gacttaaacg tgaaatttat
    21301 gaagaaacag gcatttattg tactgtgggc attggttcta atatgttatt aagtaaaatt
    21361 gctatggatg ttgaagcgaa gcatagtcaa aatggtatag ctgaatggcg atatcaagat
    21421 gtaccaacga aattatggcc aattcagccc ttgcgagatt tttggggtat taatcgtcga
    21481 acagaagcca aattgaataa aagaggaatt tttactatag gagatttagc gaaatatcca
    21541 tataaatttt taaaaaaaga gttcggtatt ttaggtgttg atatgcatct acatgcgaat
    21601 gggatagatc agagtaaagt acgtgaaaag cacaagatca gcaatccatc gatatgcaaa
    21661 agtcaaatat taatgagaga ttatcatttt gatgaagcaa aagtagtaat gcaagagtta
    21721 attgaagatg ttgctagcag agttcgagca agaaaaaaag tggcaagaac gatacatttt
    21781 gcctttggct atagtgatga aggcggtgta cataagcaat atactttgaa agatccaaca
    21841 aacttagaaa aagatattta taaagtagta atgcatttcg cagataaatt atgtaataaa
    21901 caagcactat atcgtacgct aagtatatct ttgagtcaat ttattaatga ggatgagcga
    21961 cagttaagtc tgtttgaata tgaataccaa cgcaaacgtg acgaatgtct agctaaaacg
    22021 atagaccaat tacatttgaa atacggcaaa ggtattgtgt ccaaagcagt atcgtttaca
    22081 gaagcaggta caaaacacgg cagattaggt ttaatggctg gacataaaat gtaatgatta
    22141 tacggtttaa ggaatatata actgtgattc gtataaaata agtctctaaa gataaatatt
    22201 tcatatatca caatagattt cacaataata tctaagaata catgggaatt tatcaaaaga
    22261 gacttaataa ttattggata taacaatcaa aatcactcaa tgcttgcata ccgcgttctc
    22321 ggtcagtagg atttttgaaa ctaattttta aagcaccgta tatatcttcg cgtacttcta
    22381 agattcttaa gttgcttata gatatgttat gtaaactcag gatataagtc actttactta
    22441 tcatacctga ttcatccgga atatccacat atagatcata cgcagtattt agtccaccta
    22501 gttgtttagc gggcagtgcg tcacgatacg atttagcttc ggcaaaaaat gataacaatt
    22561 tttcagaatc attgctttca attagtcttt ctaaatcttg aaactgactt tttagctgtc
    22621 gaatcatttc taaaatatac gttttattac tcaaggtgat atctttccac atttgtgcat
    22681 tactactagc tatacgagtg atattacgaa aaccaccagc tgcaagttta ttgactaaat
    22741 gatgttcttg accgttcttt tgactaacat gaactaaact agatgcaacg atatgaggta
    22801 aatgacttac gacgcttgtt acgtagtcgt gttcttcagc agtagttaca ataaatttag
    22861 caagagtagg tgataacagt tcttttaacg tgtttgctgc ttgctcattt cttgcctcat
    22921 tgtagactaa aatataataa gcgttttcaa ataagtgctt tttagcattt agtacaccag
    22981 atttatgact accagccatt ggatgaccac tgactagatg aatattatgc tttaataaat
    23041 tgcattcgtg ttgctgtatc attgctttag tactaccagt atcagaaaca ataacaccag
    23101 gtttagttgg catatctata agcttgctaa gatatttatt tgtgatagca acaggtgttg
    23161 cataaataat tacatcggct tttttaatag cttcactata atttaaacat ttttcattaa
    23221 taataccgat tgatttagct ttatctaact gagaagtatc tgcatcgtat gcaataatat
    23281 tagtattagg gttatggtat tttatattgc tagcaagact tccaccaatt aatccaagcc
    23341 caacaaataa aactgttgtc atataaatca ccttatttcg aaattttcag aataataaca
    23401 ttgtaaatga gctgttgaca cagtgcaata gtaaataaaa atcgataata gcattaatag
    23461 ataaacggag ataaatcatc tacaataaag agtatagtaa cacaatggca acggaggggt
    23521 aaatcaatgg aaccaatatt agaaatgatt aaaacattaa caggtattaa tagtccttca
    23581 ggagacacag aagaagcaat tcaatttgtc gaaaaatatg caaaagactt gggttatcaa
    23641 acaacactaa caaataaagg tgcgttatta ataacagtgc caggcaaaaa tgatgaagta
    23701 caacgctgta ttactgctca tgttgatact ttaggtgcaa tggttaaaga aattaaagaa
    23761 gatgggcgct tagcaataga attaattgga ggattcacgt ataacgcgat tgagggtgaa
    23821 tattgccaaa ttaaaactga tgctggtcaa atatatacag gaacaatttg tctgcatgaa
    23881 acaagtgttc atgtatataa aaataatcat gaaataacta gagaccaaaa gcatatggaa
    23941 ataagagttg atgaagtaac tacatcagaa gaagatacaa agagtttagg tatttcagta
    24001 ggtgattttg ttagctttga tccacgtaca gttatcacgt catcaggttt cattaaatct
    24061 cgccatttag atgataaagc tagcgtagcg atgatactac aattactaaa gaaattaaaa
    24121 gaagagcaaa taatattacc acatacaacg caattttata tttctaataa cgaagaaata
    24181 ggttacggtg caaatgcatc aattgattcg aaaatcaaag aatatattgc attagatatg
    24241 ggcgcgttgg gagacggtca agcatcggat gaatatacag tttctatttg tgccaaagat
    24301 gcttcaggtc catatcataa gcaattgaaa tcgcacctag ttaatctttg caaaataaat
    24361 aacattccat ataaagtcga catatatcca tattatggtt cagatgcttc agcagcttta
    24421 catgctggtg cggatatcag acatggttta tttggcgctg gcattgaatc atctcatgca
    24481 atggaacgaa cacatattga ttctattaaa gcgacagaga aattactata tgcatattgc
    24541 ttatcaccaa ttgagtaaac aattagtgtt gacaaatgtg aacgacctat gtaatataat
    24601 gaactataaa aataattaga attttctaaa gaaatagtag cagatatgaa acgtagcaaa
    24661 tagaaagcta atgggtgatg ggaattagca cgccatatct tgtgaattgg actttggaaa
    24721 acaattgaat gagttttgaa agtgaacatg aattatgtta actaaggtgg caccacggta
    24781 acgcgtcctt acaggtatat gcgttatgtg gtgtcttttt atttagacaa aatgtagtag
    24841 ttaattaaag gtagcaacag aaagttagtg gatgatgtga actaacaccg agattaatga
    24901 aattgggttt tgtctgcaac agaaaaatta tatatagtaa agagtgaact atgaatattt
    24961 cgaatattcg gttaatttag gtggtaccac gcgtcagcgt cctttatatt gataaggatg
    25021 ctggcgcttt tttgaaagga gcgtatagaa tggatatatt ttataaaaaa ataaaagcaa
    25081 atgtaacgcc agaagtttta gcacaacttc attccaagaa gatcattttg gaaagtacaa
    25141 atcaacaaca aactaaaggt cgctattcag ttgttatttt tgatatttat ggcactttaa
    25201 ctttagataa tgatgtatta tcagtaagca ctttaaaaga atcgtatcaa atcattgaaa
    25261 gaccgtacca ttatttaacg actaaaataa atgaagacta ccataatatt caagatgagc
    25321 aacttaagtc attaccgttt atatctggat atgttgggac gtgtagcttt gatttagtaa
    25381 gacatgaatt tcctaaattg caatcaatac aattagaaga tcacaagcag cacgatgtaa
    25441 ggttatatat ggttgaacaa gtttatgtat ttgaccatta caaagatgag ttatatatca
    25501 tcgcgacgaa tcaattttca aattcaacaa aatcagatct tgagaatcga gttaataagt
    25561 ctatcgaaga cttaactaaa atccaaccat tcatgcctac acaagatttt gattttaaaa
    25621 ctaaagaaat tcaatcaaat atttctgaag aaagatttat cgaaatgatt cagtatttca
    25681 aagagaaaat aacagaaggg gatatgttcc aagttgtgcc atcaagaatt tacaaatatg
    25741 cgcatcatgc tagtcagcat ttaaatcaac tttcgtttca actgtatcaa aatttaaaac
    25801 gacaaaaccc aagtccatat atgtattatc ttaatatcga taaaccctat attgtcggta
    25861 gttctcccga aagtttcgta agtgtcaaag atcaaattgt aacaactaat cctattgcag
    25921 gtacgattca acgtggtgag acgacacaaa tagataatga gaatatgaaa caactactta
    25981 atgatccaaa agaatgcagc gaacatcgta tgctagttga tttaggacgt aatgatattc
    26041 atagagtaag taaaatcggt acctcaaaaa ttactaaatt aatggttatt gaaaaatatg
    26101 aacatgttat gcatatcgta agtgaaatca caggtaaaat aaatcaaaat ttatcaccaa
    26161 tgacagttat tgcgaattta ttaccaacag gtaccgtttc aggtgcacca aaattacgtg
    26221 caattgaaag aatatatgaa caatatccac ataaacgagg cgtttatagt ggtggtgttg
    26281 gatacataaa ttgtaatcat aacttagatt ttgctttagc aattcgaacg atgatgatag
    26341 atgagcagta tatcaacgta gaagctggtt gtggcgttgt atatgattct attcctgaaa
    26401 aagaactaaa tgaaacgaaa ttgaaagcta aaagcttatt ggaggtgagc ccatgatctt
    26461 agttgtagat aattatgatt cctttacata taacctagtg gatattgttg ctcaacatac
    26521 tgacgtcatt gttcaatacc ctgatgatga taatgtgctg aatcaatcgg tggacgctgt
    26581 tattatatct cctggtccag ggcatccatt agacgatcaa cagttgatga aaatcatatc
    26641 aacctatcaa cacaaaccca ttttaggtat atgtttaggg gctcaggcac tgacttgtta
    26701 ctacggtgga gaagtcatta aaggcgacaa ggttatgcac ggcaaagttg atactataag
    26761 tatcacttca aatactcaca aagtgttata tcaagatgtt ccagaacagt tttcaattat
    26821 gagatatcat tcattaataa gtaaccctga caattttcca gaagaattga aaattactgg
    26881 acgtaccaaa gattgtatac agtcatttga gcataaagaa agaccgcatt acggtattca
    26941 gtaccatcct gaatcatttg ctacagacta tggtgtcaaa ataattacaa attttattaa
    27001 tctagtgaag gaaggatgaa aaccatgaca ttactaacaa gaataaaaac tgaaactata
    27061 ttacttgaaa gcgacattaa agagctaatc gatatactta tttctcctag tattggaact
    27121 gatattaaat atgaattact tagttcctat tcggagcgag aaatccaaca acaagaatta
    27181 acatatattg tacgtagctt aattaataca atgtatccac atcaaccatg ttatgaaggg
    27241 gctatgtgtg tgtgcggcac aggtggtgac aagtcaaata gtttcaacat ttcaacgact
    27301 gttgcttttg ttgtagcaag tgctggcgta aaagttataa aacatggtaa taaaagtatt
    27361 acctcaaatt caggtagtac ggatttgtta aatcaaatga acatacaaac aacaactgtt
    27421 gatgatacac ctaaccaatt aaatgaaaaa gaccttgtat tcattggtgc aactgaatca
    27481 tatccaatca tgaagtatat gcaaccagtt agaaaaatga ttggaaagcc tacaatatta
    27541 aaccttgtgg gtccattaat taatccatat cacttaacgt atcaaatggt aggcgtcttt
    27601 gatcctacaa agttaaagtt agttgctaaa acgattaaag atttaggtag aaaacgtgca
    27661 atcgttttac atggtgcaaa tggtatggat gaagcaacac tatctggtga taatttgata
    27721 tatgaattga ctgaagatgg agaaatcaaa aattacacat taaatgcgac tgattatggt
    27781 ttgaaacatg cgccgaatag tgattttaaa ggcggttcac ctgaagaaaa tttagcaatc
    27841 acactgaata tcttgaatgg taaagatcag tcaagtcgac gtgatgttgt cttactaaat
    27901 gcgggtttaa gcctttatgt tgcagagaaa gtggatacca tcgcagaagg catagaactt
    27961 gcaactacat tgattgataa tggtgaagca ttgaaaaaat accatcaaat gagaggtgaa
    28021 taatatgacg attttatcag aaattgttaa atataaacag tcacttttac aaaatggcta
    28081 ttatcaagac aaacttaata ccttgaaaag tgtgaagatt cagaataaaa aatcttttat
    28141 aaacgcaatt gagaaagaac caaagctagc aattattgca gaaatcaaat cgaagagtcc
    28201 tacagttaat gacttacctg aacgagattt atcgcaacaa atctcagatt atgaccaata
    28261 tggtgcaaat gccgtgtcta ttttaactga tgaaaagtac tttgatggta gttttgaaag
    28321 attacaagca ttgacgacaa aaacaacatt acccgtatta tgcaaagact ttattataga
    28381 cccgcttcaa attgatgttg ctaaacaagc tggtgcatct atgattttat tgatcgttaa
    28441 catcttatct gataaacaat tgaaagattt atataactac gctatatcgc aaaatctaga
    28501 agtgttagtt gaagtacatg atcgccatga attagaacgt gcctataagg ttaatgctaa
    28561 attgattggt gtgaataaca gggacttaaa acgatttgtc acaaatgtgg aacatacaaa
    28621 tactatttta gaaaataaaa aaccaaatca tcattatatt tctgaaagtg gtattcacga
    28681 tgcatctgat gtaagaaaaa tcttgcatag tggtatcgat ggcttactaa taggtgaggc
    28741 gcttatgcgt tgtgacaata tatctgaatt tttaccacaa ctgaaaatgc aaaaggtgaa
    28801 gtcatgatga aattgaaatt ttgtggcttt acatcaataa aggatgttac agcggccagt
    28861 caattaccta ttgatgcgat aggtttcatc cattatgaaa aaagtaaaag gcatcaaaca
    28921 attacccaaa taaaaaagtt agcgtctgct gttccaaatc atatcgataa agtatgtgtc
    28981 atggtaaatc ctgatttaac aacaattgaa cacgtattaa gcaatacgtc aattaacaca
    29041 atacagttac acggcacaga atctattgat tttatacagg aaattaaaaa gaaatattca
    29101 agcattaaaa tcactaaagc tttagctgca gatgaaaaca taatccaaaa cataaataaa
    29161 tataaagagt tcgtagattt atttattatc gacacaccct cagtgtcgta tggtggtacc
    29221 ggtcaaacat atgactggac tattttgaag cacataaaag acatacctta cttgatagca
    29281 ggaggcatta actctgaaaa tattcaaaaa gttaatcaac ttaaattatc acatcaaggt
    29341 tatgatcttg catcaggtat agaagtaaat gggcgaaaag atatagaaaa aatgacagca
    29401 attgtaaata ttgtgaaagg agatagagaa aatgaataaa caaatacaaa cagaagcaga
    29461 tgaattaggt ttctttggtg aatacggagg gcaatatgtt ccagaaacat taatgccagc
    29521 aattattgag ttgaaaaaag cttataaaga ggcaaaagca gacccagagt ttcaaagaga
    29581 actggaatac tatttatcag agtatgtagg acgcgcgacg ccacttacat atgctgcatc
    29641 atatactgaa agcctaggtg gcgctaaaat atatttgaaa cgagaggatc taaatcatac
    29701 aggcgcccat aaaattaata atgcgttagg tcaagcgttg cttgctaaaa gaatgggcaa
    29761 gaagaagctt gttgctgaaa ctggtgcggg tcaacatggt gtagctagtg ctacggttgc
    29821 tgcattattt gatatggaac ttgttgtctt tatgggaagt gaagatatta aaagacaaca
    29881 acttaatgta tttagaatgg aattacttgg tgcaaaggtt gtggcagttg aagatggtca
    29941 agggacttta tcggatgcag ttaataaagc attgcaatat tgggtaagtc atgtagatga
    30001 tacacattat ttattaggtt ctgcattagg tccagacccg ttcccaacga ttgttagaga
    30061 ttttcagagt gtgattggta aagaaataaa atcacagata ttgaagaaag aaggtcgact
    30121 tccggatgca attgtagcat gtatcggtgg tggctcaaat gcaatcggta cattttatcc
    30181 atttattaaa gatgatgttg cattatacgg tgttgaagcc gcaggtcaag gcgaggatac
    30241 tgataaacat gcacttgcaa ttggcaaagg atcacctggc gtattacatg gtactaaaat
    30301 gtatttaatt caagatgaag gtgggcaagt gcaactagca cattctattt cagcaggact
    30361 tgattatcct ggtattgggc cagaacattc ttattaccac gacattggta gagtaacttt
    30421 tgaaaatgca agtgatacac aagcaatgaa tgctttaatc aactttacaa aacatgaagg
    30481 tattatacct gcaattgaaa gtgcacatgc actgagttat gttgaaagac tagcgcctac
    30541 gatgtcgaaa gaagatatta ttgtagtaac tatttctgga cgtggcgata aagatatgga
    30601 aacaattaga caatatatgg cagagcgagg tcttgcaaat gactaaatta tttatacctt
    30661 atattatggg caataaagat ttgattgaaa atgcaacatt gttgagtgaa aatggtacag
    30721 atataattga aattggtgta cctttctctg atccggttgc tgatggtcca gttatcatgg
    30781 aagcaggtca acaggcgatt aaacaaggca tcacgataga ttatattttc aatcaattag
    30841 aaaaacatgg tgatcaaatt aagtgtaact atgtattaat gacgtattat aatattattt
    30901 gtcattatgg agaacaagcg ttttttgaaa aatgtcgaga tactggtgtc tacggcttaa
    30961 ttattcctga tttaccatat gaattatcgc agcgtttaaa agaacaattt agtcactatg
    31021 gcgtcaaaat catatcgtta gttgcgatga ctactgatga caaacgtatg aaagatatcg
    31081 tatcccatgc ggaaggcttt atttatactg tgacgatgaa tgcgacaaca gggcaaaacg
    31141 gtgcgtttca tccagaatta aaacgaaaaa ttgagtcaat taaagcgata gccaatgtgc
    31201 cagttgtcgc aggatttggt ataagaacac ctcaacatgt tgcagatata aaagaggttg
    31261 cagatggcat cgtcattggt agcgaaatcg ttaagcgatt taaatctaac acgcgtgaag
    31321 aaatcattaa atatttacaa tctatccaac aaacattgaa taattaagtt tacttgattt
    31381 aaaaaaatta gtcgaatact gtttgaaaaa gtgaaaaacg gtgaattata aaattgaata
    31441 gaaatgatac aaaagtaata tgagcaaacc caaacgttca tattactttt tttgaaattg
    31501 tatgcaaaaa tctaaatatt actataaaag tatacgcaat taaagcgttt atgttttagt
    31561 tttaacatta actattgtat acttatttag attagattta ttatttttga catttgcaga
    31621 ggggaaatag aaaaactgca aatacggaaa tgaaattaat taacgagaga caaataggag
    31681 taatgataat gaagtttaca aatttaacag ctaaagagtt tggtgccttt acagatagca
    31741 tgccatacag tcatttcacg caaactgttg gccactatga gttaaaactt gctgaaggtt
    31801 atgaaacaca tttagtggga ataaaaaaca ataataacga ggttattgca gcttgcttac
    31861 ttactgctgt acctgttatg aaagtgttca agtattttta ttcaaatcgc ggtccagtga
    31921 tcgattatga aaatcaagaa ctcgtacact ttttctttaa tgaattatca aaatatgtta
    31981 aaaaacatcg ttgtctatac ctacatatcg atccatattt accatatcaa tacttgaatc
    32041 atgatggcga gattacaggt aatgctggta atgattggtt ctttgataaa atgagtaact
    32101 taggatttga acatactgga ttccataaag gatttgatcc tgtgctacaa attcgttatc
    32161 actcagtgtt agatttaaaa gataaaacag cagatgacat cattaaaaat atggatggac
    32221 ttagaaaaag aaacacgaaa aaagttaaaa agaatggtgt taaagtaaga tatttatctg
    32281 aagaagaact gccaattttt agatcattta tggaagatac gtcagaatca aaagcttttg
    32341 ctgatcgtga tgacaaattt tactacaatc gcttaaaata ctacaaagac cgtgtgttag
    32401 tgcctttagc gtatatcaac tttgatgaat atattaaaga actaaatgaa gagcgtgata
    32461 ttttaaataa agatttaaat aaagcattaa aggatattga aaaacgtcct gaaaataaaa
    32521 aagcgcataa caagcgagat aacttacaac aacaacttga tgcaaatgag caaaagattg
    32581 aagaaggtaa acgtctacaa gaagaacatg gtaatgaatt acctatctct gctggtttct
    32641 tctttatcaa tccatttgaa gttgtttatt atgctggtgg tacatcaaat gcattccgtc
    32701 attttgccgg aagttatgca gtgcaatggg aaatgattaa ttatgcatta aatcatggca
    32761 ttgaccgtta taatttctat ggtgttagtg gtaaatttac agaagatgct gaagatgctg
    32821 gtgtagttaa attcaaaaaa ggttacaatg ctgaaattat tgaatatgtt ggtgacttta
    32881 ttaaaccaat taataaacct gtttacgcag catataccgc acttaaaaaa gttaaagaca
    32941 gaatttttta ggaagggaat tatcaaaaca tgaaatttac agagttaact gttaccgaat
    33001 ttgacaactt tgtacaaaat ccatcattgg aaagtcatta tttccaagta aaagaaaata
    33061 tagttacccg tgagaatgat ggctttgaag tagttttatt aggtattaaa gacgacaata
    33121 acaaagtaat tgcagcaagc cttttctcta aaattcctac tatgggaagt tatgtttact
    33181 attcgaatcg tggtccagta atggattttt cagatttagg attagttgat tattatttaa
    33241 aagagttaga taaatattta cagcaacatc aatgtttata tgttaaatta gatccgtatt
    33301 ggttatatca tctatatgat aaagatatcg tgccatttga aggtcgcgag aaaaatgatg
    33361 ccttagtaaa cttgtttaaa tcacatggtt acgagcatca tggctttaca actgagtatg
    33421 atacatcgag ccaagtacga tggatgggcg tattaaacct tgaaggtaaa acacccgaaa
    33481 cattgaaaaa gacatttgat agtcaacgta aacgtaatat taataaagcg ataaactatg
    33541 gtgttaaagt cagattcctt gaacgtgatg agttcaatct tttcttagat ttatatcgtg
    33601 aaactgaaga gcgtgctgga tttgtatcaa aaacagatga ttatttttat aactttattg
    33661 acacatatgg agataaagta ttagtaccat tagcatatat tgaccttgat gaatatgtgt
    33721 taaagttgca acaggaattg aatgacaaag aaaatcgtcg tgatcaaatg atggcgaaag
    33781 aaaacaaatc agataagcaa atgaagaaaa ttgcagaatt agataagcaa attgatcatg
    33841 atcagcatga attattgaat gcaagtgaat tgagcaaaac ggacggccca attctaaacc
    33901 ttgcttctgg cgtttatttt gcaaatgcat atgaagtgaa ttatttctct ggtggttcat
    33961 cagaaaaata taatcaattt atgggaccat acatgatgca ttggtttatg attaactatt
    34021 gcttcgataa tggttatgat cgttataatt tctatggttt atcaggtgat ttcacagaaa
    34081 acagtgaaga ttatggcgta taccgcttta aacgtggatt taatgtacaa atcgaagaat
    34141 taatagggga tttctataaa ccaattcata aagtgaaata ttggttgttc acaacattgg
    34201 ataaattacg taaaaaatta aagaaataga tgtaaatgat gttagggctt atagttattg
    34261 atgctatagg ctctttttta tatgtttttt agcgaaattt taattatttc acactaagaa
    34321 aaactagtat gtatttgtca atctaatgtt tttcattata atgctgtttt attgatttaa
    34381 ttttatgatt aaaatattat gcaaaacata aaatatatag cgtcatattt gagttttaat
    34441 gataa
//
LOCUS       DAHHOJ010000017        33041 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.017, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000017 DAHHOJ010000000
VERSION     DAHHOJ010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 33041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.017"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            60..869
                     /locus_tag="NBH32_000697"
     CDS             60..869
                     /locus_tag="NBH32_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HCD1514325.1"
                     /translation="MDFSNFFQNLSTLKIVTSILDLLIVWYVLYLLITVFKGTKAIQL
                     LKGILVIVIGQQISMILNLTATSKLFDIVIQWGVLALIVIFQPEIRRALEQLGRGSFL
                     KRYTSNTYSKDEEKLIQSVSKAVQYMAKRRIGALIVFEKETGLQDYIETGIAMDSNIS
                     QELLINVFIPNTPLHDGAMIIQGTKIAAAASYLPLSDSPKISKSLGTRHRAAVGISEV
                     SDAFTVIVSEETGDISVTFDGKLRRDISNEIFEELLAEHWFGTRFQKKGVK"
     gene            871..1803
                     /locus_tag="NBH32_000698"
     CDS             871..1803
                     /locus_tag="NBH32_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HCD1514326.1"
                     /translation="MLESKWGLRFIAFLLALFFFLSVNNVFGNIFNTGNLGQKSSKTI
                     QDVPVEILYNTKDLHLTKAPETVNVTISGPQSKIIKIENPEDLRVVIDLSNAKAGKYQ
                     EKYQVKGLADDIHYSVKPKLANITLENKVTKKMTVQPDVSQSDIDPLYKITKQEVSPQ
                     TVKVTGGEEQLNDIAYLKATFKTNKKINGDTKDVAEVTAFDKKLNKLNVSIQPNEVNL
                     QVKVEPFSKKVKVNVKQKGSLADDKELSSIDLEDKEIEIFGSRDDLQNISEVDAEVDL
                     DGISESTEKTVKINLPEHVTKAQPSETKAYINVK"
     gene            1830..3185
                     /locus_tag="NBH32_000699"
     CDS             1830..3185
                     /locus_tag="NBH32_000699"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCD1514327.1"
                     /translation="MGKYFGTDGVRGVANQELTPELAFKLGRYGGYVLAHNKGEKHPR
                     VLVGRDTRVSGEMLESALIAGLISIGAEVMRLGIISTPGVAYLTRDMGAELGVMISAS
                     HNPVADNGIKFFESDGFKLSDEQENEIEALLDQENPELPRPVGNDIVHYSDYFEGAQK
                     YLSYLKSTVDVNFEGLKIVLDGANGSTSSLAPFLFGDLEADTETIGCSPDGYNINEKC
                     GSTHPEKLAEKVVETESDFGLAFDGDGDRIIAVDENGQIVDGDQIMFIIGQEMHKNQE
                     LNNDMIVSTVMSNLGFYKALEQEGIKSNKTKVGDRYVVEEMRRGNYNLGGEQSGHIVM
                     MDYNTTGDGLLTGIQLASVIKMTGKSLSELAGQMKKYPQSLINVRVTDKYRVEENVDV
                     KEVMTKVEVEMNGEGRILVRPSGTEPLVRVMVEAATDEDAERFAQQIADVVQDKMGLD
                     K"
     gene            3462..10778
                     /locus_tag="NBH32_000700"
     CDS             3462..10778
                     /locus_tag="NBH32_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1542 domain-containing protein"
                     /protein_id="HCD1514328.1"
                     /translation="MNLFRQQKFSIRKFNVGIFSALIATVTFISINPTTASAAEQNQP
                     AQNQPAQPADPNAQPNANAGAQANPTAQPAAPANQGGQADPAANQGGQANPAGGAVQP
                     AAGTTQPAGQGNQADPNNAAQAQPGNQAAPANQAGQGNNQATPNNNATPANQTQPANA
                     PAGAQPAAAVAANGQTQDPNASNTGEGSINTTLTFDDPAISTDENRQDPTVTVTDKVN
                     GYSLINNGKIGFVNSELRRSDMFDKNNPQNYQARGNVAALGRVNANDSTDHGNFNGIS
                     KTVNVKPDSELIINFTTMQTNSKQGATNLVIKDAKKNTELATVNVAKTGTAHLFKVPT
                     DADRLDLQFIPDNTAVADASRITTNKDGYKYYSFIDNVGLFSGSHLYVKNRDLAPKAT
                     NNKEYTINTEIGNNGNFGASLKADQFKYEVTLPQGVTYVNDSLTTTFPNGNEDSTVLK
                     NMTVNYDQTANKVTFTSQGVTTARGTHTKEVLFPDKSLKLSYKVNIANIDTPKNIDFN
                     EKLTYRTASDVVINNAQPEVTLTADPFSVAVEMNKDALQQQVNSQVDNSHYTTASIAE
                     YNKLKQQADTILNEDANHVETANRASQADIDGLVTKLQAALIDNQAAIAELDAKAQEK
                     ITAAQQSKKVTQDEVAALVTKINNDKNNAIAEINKQTTAQGVTTEKDNGIAVLEQDVI
                     TPTVKPQAKQDIIQAVTTRKQQIKKSTASLQDEKDVANDKIGKIETKAIKDIDAATTN
                     AQVEAIKTKAINDINQTAPATTAKAEALEEFDEVVQAQIDQAPLNPDTTNEEVAEAIE
                     RINAAKVSGVKAIEATTTAQDLERVKNEEIFKIENITDSTQTKMDAYKEVKQAATARK
                     AQNATVSNATDEEVAEANAAVDAAQTEGLHDIQVVKSQQEVADTKAKVLDKINAIQTQ
                     SRVKPAADTEVDNAYNTRKQEIQNSNASTTEEKEAAYTELDAKKQEARTNLDAANTNS
                     AVTTAKDNGIAAINQVQAATTKKSDAKAEIAQKASERKTAIEAMNDSTSEEQQAAKDK
                     VDQAVVTANADIDNAAANSDVDNAKTTNEATIAAITPDANVKPQAKQAIADKVQAQET
                     AIDANNGAITEEKAAAKQQVQTEKTTADTAIDGAHSNAEVEAAKNAEIAKIEAIQPAT
                     TTKDNAKQAIATKANERKTAIAQTQDITAEEIAAANADVDNAVIQANSNIEAANSQND
                     VDQAKTTGETSIDQVTPTVNKKATARNEITTTLNNKLQEIQATPDATDEEKQDAETEA
                     NTENAKANQAITAATTNAEVDEAKTNAEAAINAVTPKVMKKQAAKDEIDQLQAAQTAV
                     INNDQNATNEEKEAAIQQLATAVTDAKNNITAATDNNGVDTAKDAGKNSIQSTQPATA
                     VKSNAKNDVDQAVTTQNQAIDNTTGATTEEKNAAKDLVSKAKEKAYQDILNAQTTNDV
                     TQLKDQAVADIQGITADTTIKDVAKGELTAKANEQKALIAQTADATTEEKEQANQQVD
                     AQLTQGNQNIENAQSIDDVNTAKDNAVQAIDPIQASTDVKTNARAELLNEMQNKITEI
                     LNDNTTTNEEKGKDIGPVRAAYEEGLNNINVANSTGDVTTAKDAAVQKVQQLHANPVK
                     KPAGKTELDQAAADKKTQIEQTPNASQQEINDAKQEVDTELNQAKTNIDQSSTDDYVD
                     AAVRDGKAKINAVKTFSEYKKDALAKIADAYNAKVNEADNSNASTSSEIAEAKQKLAE
                     LKQTADQNVNQATSKDDIEVQIHNDLDNINDYTIPTGKKESATTDLYAYADQKKNNIS
                     ADTNATQDEKQQAIKQVDQNVQTALESINNGVDNSDVDDALTQGKATIDAVQVDATVK
                     AKANQAIDAKAQETKESIDQSDQLTAEEKTEALATIKQITDQAKQGITDATTTAEVEK
                     AKAQGLEAFDNIQIDSTEKQKAIEELETALDQIEAGVNVDSDATTEEKEVFTNALEEI
                     LSKATEDISDQTTNAEIATVKNSALEQLKAQRINPEVKKNALEAIREVVNKQIEIIKN
                     ADADASAKEIARTDLGRYFDRFADDLDKTQTNAEVAELQNVTIPAIEAIVPQNDPDAN
                     HSSSDNNDATANTNVNAEPDNAGQPNVTESTDNANADTSSTTTNNHNDAATGETTATS
                     TNNSSTDDANDKPTANNNSSVDASIDNSATGNGTTDKPATTESTEKTSSADSKDNASV
                     NDSNQNAEVNNSAESQPTNGKVAQPKSENKAKVEKDGRDSTNQSMVESTTETLPSADI
                     TEPNVPSNTSKDKEESTSNQTDAGQLKSETNVASNEADKSPSKADTEVSNKPSTSASS
                     EAKDKMTSTEDSQKADMATADTKDNQASIGSSADANNKATQNDGANASPATVSNGSNS
                     ANQDMLNVTKPEENKANAKSDQQGKVNKPKQQAKTLPDTGMSHNDDLPYAELALGAGM
                     AFLIRRFTKKDQQTEE"
     gene            complement(11097..12203)
                     /locus_tag="NBH32_000701"
     CDS             complement(11097..12203)
                     /locus_tag="NBH32_000701"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCD1514329.1"
                     /translation="MKAVHFGAGNIGRGFIGYILADNNVKVTFADVNEEIINALAHDH
                     QYDVILADESKTTTRVNNVDAINSMQPSEALKQAILEADIITTAVGVNILPIIAKSFA
                     SFLKEKTNHVNIVACENAIMATDTLKKAVLDITGPLGNNIHFANSAVDRIVPLQKNEN
                     ILDVMVEPFYEWVVEKDAWYGPELNHIKYVDDLTPYIERKLLTVNTGHAYLAYAGKFA
                     GKATVLDAVKDSSIEAGLRRVLAETSQYITNEFDFTEAEQAGYVEKIIDRFNNSYLSD
                     EVTRVGRGTLRKIGPKDRIIKPLTYLYNKDLERTGLLNTAALLLKYDDTGDQETVEKN
                     NYIKEHGLKAFLSEYAKVDDGLADEIIEAYNSLS"
     gene            complement(12203..12637)
                     /locus_tag="NBH32_000702"
     CDS             complement(12203..12637)
                     /locus_tag="NBH32_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1514330.1"
                     /translation="MSELFSNDNIFLNVNVNSQNEAIEKAGKALVDSGAVTDAYIQAM
                     KDREQVVSTFMGNGLAIPHGTDEAKTNVIHSGLTLLQIPEGVDWDGEVVKVVVGIAGK
                     DGEHLDLLSKIAITFSEEENVDRIVQAKSAEEIKQVFEEADA"
     gene            complement(12649..14781)
                     /locus_tag="NBH32_000703"
     CDS             complement(12649..14781)
                     /locus_tag="NBH32_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1514331.1"
                     /translation="MLLSTREKEMIALLIKYHGQYITIHDIAQQLAVSSRTIHRELKG
                     VESYLTSFSLTLERANKKGLRIAGADSDLNDLKQSIAQLQTIDLSVEEQKVIIIYALI
                     QAKEPVKQYSLAQEIGVSVQTLAKMLDDLELDLNKYQLSLSRKRGEGIYLVGTESKKR
                     EFLSQLMVNNLNSTSVYSVIENHFVFHSLNQIHKDFVDLERIFNVERLLMDYLSALPY
                     QLTESSYLTLTVHIVLSISRIKNGEYVALNDDIYDSVQNTFEHKVASELADKLDQIYD
                     VTFNQAEIAFITIHLRGAKRKNLNDTSLNNRCEENKIKAFVNKVEMISGMTFADLDTL
                     VDGLTLHLNPAINRLQANIETYNPLTDMIKFKYPRLFENVRLALNDCWPDLIFPESEI
                     AFIVLHFGGSIKNQGNRFLNILVVCSSGMGTSRLLSTRLEQVFSEIERITQASVSDLK
                     SLDLSQYDGIISTVNLDIDTPYLMVNPLLPDSDISYVAQFLNTKSTFQETHDKSSNMN
                     DKDDVHVETKDIDGNTSFENEQTSNLTSVFEKHLSDEKSEQLMHHMRSGLTLLDSVKI
                     VSTEVKQWQTYIADYLYQCDAIKDPTSFAELLEQRLIDNPGWILSPYPVAIPHLRDNM
                     IKHPMILITVLEEPLTLPSIQNDNQTIKYMISMFIPDNDFMASLVSDLSEFLSLKLES
                     IDTFMENPQELETLLRSKFLERIKKQFI"
     gene            complement(14816..16354)
                     /locus_tag="NBH32_000704"
     CDS             complement(14816..16354)
                     /locus_tag="NBH32_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICB"
                     /protein_id="HCD1514332.1"
                     /translation="MSQTEEKKGIGRRVQAFGSFLSSMIMPNIGAFIAWGFIAAIFID
                     NGWLPNKDLATLAGPMITYLIPLLIAFSGGRLIYDLRGGIIAATATMGVIVALPDTPM
                     LLGAMIMGPLVGWLMKKTDQLIQPRTPQGFEMLFNNFSAGILGFIMTIAGFKILAPIM
                     KFIMHILSVAVESLVHAHLLPLVSILVEPAKIVFLNNAINHGVFTPLGADQAAKAGQS
                     ILYTIESNPGPGLGILLAYMIFGKGTAKATSYGAGIIHFLGGIHEIYFPYVLMRPLLF
                     IAVILGGMTGVATYQATGFGFKSPASPGSFIVYCLNAPRGEFLHMLLGVFLAALVSFV
                     VATLIMKFTREPKQDLEAATAQMENTKGKKSSVASKLVSSDKNVNTEENASGNVSETS
                     SSDDDPEALLDNYNTEDVDAHNYNNINHVIFACDAGMGSSAMGASMLRNKFKKAGIND
                     ITVTNTAINQLPKDAQLVITQKKLTDRAIKQTPNAIHISVDNFLNSPRYEELLNNLKK
                     DDQA"
     gene            17035..18840
                     /gene="glmS"
                     /locus_tag="NBH32_000705"
     CDS             17035..18840
                     /gene="glmS"
                     /locus_tag="NBH32_000705"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCD1514333.1"
                     /translation="MCGIVGYIGYDNAKELLLKGLEKLEYRGYDSAGIAVVNDDNTTV
                     FKEKGRIAELRKVADSSDFDGPVGIGHTRWATHGVPNHENSHPHQSSNGRFTLVHNGV
                     IENYEELKGEYLQGVSFISETDTEVIVQLVEYFSNQGLSTEEAFTKVVSLLHGSYALG
                     LLDAEDKDTIYVAKNKSPLLLGVGEGFNVIASDALAMLQVTSEYKEIHDHEIVIVKKD
                     EVIIKDADGNVVERDSYIAEIDASDAEKGVYAHYMLKEIHEQPAVMRRIIQEYQDAEG
                     NLKIDQDIINDVKEADRIYVIAAGTSYHAGLVGKEFLEKWAGVPTEVHVASEFVYNMP
                     LLSEKPLFVYISQSGETADSRAVLVETNKLGHKSLTITNVAGSTLSREADHTLLLHAG
                     PEIAVASTKAYTAQIAVLSILSQIVAKEHGREADIDLLRELAKVTTAIEAIVDDAPIM
                     EQIATDFLETTRNAFFIGRTIDYNVSLEGALKLKEISYIQAEGFAGGELKHGTIALIE
                     DGTPVVALATQENVNLSIRGNVKEVVARGAHPCIISMEGLEKEGDTYVIPHVHELLTP
                     LVSVVALQLISYYAALHRDLDVDKPRNLAKSVTVE"
     gene            19072..19854
                     /locus_tag="NBH32_000706"
     CDS             19072..19854
                     /locus_tag="NBH32_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514334.1"
                     /translation="MLIQLDQIGRMKQGKTILKNISWQIAKGDKWILYGLNGAGKTTL
                     LNILNAYEPATTGGVNLFGEMPGKVGYSAETVRQHIGFVSHSLLEKFQDGERVIDVVI
                     SGAFKSIGVYQDIDDEVRNEAHQLLKLVGISAKAQQYIGYLSTGEKQRVMIARALMGQ
                     PQVLILDEPAAGLDFIARESLLNILDSLSDSYPTLAMIYVTHFIEEITGNFTKILLLK
                     DGESVQQGLIDDILTSENMSRFFQKNVAVQRWNNRFSMAMLE"
     gene            19922..20779
                     /locus_tag="NBH32_000707"
     CDS             19922..20779
                     /locus_tag="NBH32_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1514335.1"
                     /translation="MDNVKAIFLDMDGTILHENNQASTYTKDVINRLREKGYKVFLAT
                     GRSHSEIHQLVPQDFAVNGIISSNGTIGEVDGEIIFKHGLSLAQVQQIINLAKRQQIY
                     YEVFPFEGNRVSLKEDETWMRDMIRSQDPINGVSHSEWSSRQDALAGKIDWVTKFPEG
                     EYSKIYLFSSNLEKITAFRDELKQNHVQLQISVSNSSRFNAETMAYQTDKGTGIKEMI
                     AHFGIHQDETLVIGDSDNDRAMFEFGHYTVAMKNARPEIQALTSDVTAYTNEEDGAAK
                     YLAKHFLAD"
     gene            complement(21439..21597)
                     /locus_tag="NBH32_000708"
     CDS             complement(21439..21597)
                     /locus_tag="NBH32_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001792784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCD1514336.1"
                     /translation="MRKSSDALFNLLTQSFELIEGVHNELVTDNLKTVMYKPRKERTN
                     GRINRRFK"
     gene            22015..22176
                     /locus_tag="NBH32_000709"
                     /pseudo
     CDS             22015..22176
                     /locus_tag="NBH32_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002504693.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            22274..22378
                     /locus_tag="NBH32_000710"
     CDS             22274..22378
                     /locus_tag="NBH32_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514337.1"
                     /translation="MLLLERTSMSDFEMLMVVLTIIGLVLISNQGHKK"
     gene            complement(22893..23984)
                     /locus_tag="NBH32_000711"
     CDS             complement(22893..23984)
                     /locus_tag="NBH32_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCD1514338.1"
                     /translation="MFTYFKSAFKNAKPQLLITLIYALIAFAVIAVVYLLANFQLAKY
                     VQTIAIYSQFGQKPPVDAYLKVIAILLIAAVVSLFVLVQIFIGITNVMKRAMSHEKVK
                     FTDLFIAFKKGNYLKSVLIGLVSIAMIIVLSLLTSLLYKLFSPVSEMIMNSVQSSYAD
                     STHLIGIAITTQSIIIIVVLLIKAIITWLLLIPIFNFMTSFVESTNDKVKTHLANGFK
                     AMKNGQKTFFKFFIGILLLNLIIILFKTPVGYLISFNTQSLSQSVAENIIRVYTVMTI
                     ILFVVIHAIILMGIVQYYLKRGQKITKDKVKTADKDKKVVTEPKNTKVENDKVATSVE
                     TKTEKAQDSLNDNTTKTMTSDKPEDNQPK"
     gene            complement(24250..25230)
                     /gene="czrB"
                     /locus_tag="NBH32_000712"
     CDS             complement(24250..25230)
                     /gene="czrB"
                     /locus_tag="NBH32_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc efflux transporter CzrB"
                     /protein_id="HCD1514339.1"
                     /translation="MSHSHHHHDHMHSHVTTNNKKVLFISFLIIGLYMFIEIIGGLLA
                     NSLALLSDGIHMFSDTFSLGVALVAFIYAEKNATTTKTFGYKRFEVLAALFNGVTLFV
                     ISILIVFEAIKRFFVPSEVQSKEMLIISIIGLIVNIVVAFFMFKGGDTSHNLNMRGAF
                     LHVIGDLLGSVGAITAAILIWAFGWTIADPIASILVSVIILKSAWGITKSSINILMEG
                     TPSDVDIDEVITTIKKDSRIQSVHDCHVWTISNDMNALSCHVVVDHTLTMKECELLLE
                     NIEHDLLHLNIHHMTIQLETPNHKHDESIICSGTHSHSHNHHAHHHAHVH"
     gene            complement(25232..25552)
                     /gene="czrA"
                     /locus_tag="NBH32_000713"
     CDS             complement(25232..25552)
                     /gene="czrA"
                     /locus_tag="NBH32_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)-responsive metalloregulatory
                     transcriptional repressor CzrA"
                     /protein_id="HCD1514340.1"
                     /translation="MSEQYSEIHTDTLERVTEIFKALGDYNRIRIMELLSVSEASVGH
                     ISHQLNLSQSNVSHQLKLLKSVHLVKAKRQGQSMIYSLDDIHVATMLKQAIHHANHPK
                     ESGL"
     gene            25704..26369
                     /locus_tag="NBH32_000714"
     CDS             25704..26369
                     /locus_tag="NBH32_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1514341.1"
                     /translation="MNILVIGANGGVGSLLVQQLAKENVPFTAGVRQSDQLNALKSQG
                     MKAILVDVENDSIETLTETFKPFDKVIFSVGSGGNTGADKTIIVDLDGAVKSMIASKE
                     ANIKHYVMVSTYDSRRQAFDDSGDLKPYTIAKHYADDYLRRSGLNYTILHPGALTNAA
                     GSGKIEAAQYFDGKGEIPREDVATVLKEIVTSNHFNYQEFQIISGEQDIKDALTQFEI
                     EKD"
     gene            26805..27743
                     /locus_tag="NBH32_000715"
     CDS             26805..27743
                     /locus_tag="NBH32_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001620169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I mannose-6-phosphate isomerase"
                     /protein_id="HCD1514342.1"
                     /translation="MPLFLQPILKTKLWGGQRLSEFGYQLDNDTTGECWCVSAHPNGT
                     SEIINGPYQGQTLDRIWSEHRELFGDFPSKDFPLLTKIVDARESLSIHVHPDNSYAYE
                     HENGQYGKSECWYIIDAEEDAEIVIGTLAESREEVANHVQHGTIESILRYIKVKPGEF
                     YFIPAGTVHTISSGILAYETMQSSDITYRLYDFNRQDNQYNDRPLNIEKALDVIQYNA
                     PLPNILPESEIIENHKCTHIVSNDFFTLVKWEISGTLNYMKPREFCLVTVLEGEGQMI
                     VDGEIFKLTTGTNFILTSEDLDSVFEGDFTLMISYV"
     gene            27774..28004
                     /locus_tag="NBH32_000716"
     CDS             27774..28004
                     /locus_tag="NBH32_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514343.1"
                     /translation="MKQFLYIALVCGVIAGLGAFLHIPQYPSMTIPRIVAILGIISAM
                     LTFKDKQISASLKFSALLINVLPLCGTFVASN"
     gene            28678..30051
                     /locus_tag="NBH32_000717"
     CDS             28678..30051
                     /locus_tag="NBH32_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HCD1514344.1"
                     /translation="MTAETNYFWLNCGYNRWNHNEPLVGQTALFESGAHFNPSQGFRA
                     FKKAKVGDQVIFYQVQTDTGLLGCGEIISVETGAQNKIRVQFRFNEQLKPLTADYLKR
                     SEALEFRMSNMKETLFNQITAEEFDLISGLGKGEIKIPRYFFLAETEEFEPGNQYTIY
                     THTYNGIKRNGYHFYTQLEEGDNIIFYNRTKNQSVVGIGEVSKHIHEKPPIPGRTNST
                     VIEVSYEKDITPITLSTLNKHPKLKNLYFLQENAKQAIASMSQAQYDAIIEMSDNNGL
                     KSPFEMVQKPDMLESEKEEALKPFILLVVDRKEEGLKAANDLLQKANANPVITTGHPD
                     FSEDMLYGKYLPNETGALYYREGFITQLMPKKDKSYLVIDNFNRIDTDIFQTYINVLE
                     GYEVTLPRYNKDGNMIKWSRQKDSFYYFNPNWHIVGITYDSLEEIKEKYSEQFLKYTR
                     IVKVKHD"
     gene            30507..30920
                     /locus_tag="NBH32_000718"
     CDS             30507..30920
                     /locus_tag="NBH32_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol-disulfide oxidoreductase DCC family
                     protein"
                     /protein_id="HCD1514345.1"
                     /translation="MPIVYYDGNCIYCYNYVIWLIQHDLPRNYRFATLKGEVGQQFFK
                     QHPEAANKNSVILQKGDRIYFESQAIIQLITALPNRTKLLGVGLWLVPKPVRNFGYRM
                     FANNRNRMWKTTWHQPNDYEKSFFLDDNAKVKLTD"
     gene            31120..31563
                     /locus_tag="NBH32_000719"
     CDS             31120..31563
                     /locus_tag="NBH32_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HCD1514346.1"
                     /translation="MSNQQDVVKELNQQVANWTVAYTKLHNFHWYVKGPNFFSLHVKF
                     EELYNEASQYVDELAERILAVGGNPVGTLTECLEQSIVKEAAKGYSAEQMVEELSQDF
                     TNISKQLEKAIEIAGNAGDDVSEDMFIGMQTSVDKHNWMFKSYLS"
     gene            complement(31685..32395)
                     /gene="deoD"
                     /locus_tag="NBH32_000720"
     CDS             complement(31685..32395)
                     /gene="deoD"
                     /locus_tag="NBH32_000720"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1514347.1"
                     /translation="MTKGTPHIQPNGVKIAKTVLMPGDPLRAKYIADNFLENVEQFND
                     VRNMFGYTGTYKGKEVSVMGSGMGIPSIGIYSYELYNFFDVDTIIRIGSCGALQENVN
                     LYDVIIAQAASTNSNYVDQYNIPGHFAPIADFELVTKAKNVADQIGATTHVGNVLSSD
                     TFYNADPTFNAAWKKMGILGIEMESAGLYLNAIHAGKKALGIFTVSDHILRDEATTPE
                     ERQNSFTQMMEIALEIAE"
     gene            32710..>33041
                     /gene="deoC"
                     /locus_tag="NBH32_000721"
     CDS             32710..>33041
                     /gene="deoC"
                     /locus_tag="NBH32_000721"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD1514348.1"
                     /translation="MNSAKLIDHTSLKPESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YAAERLADSEVLVCTVIGFPLGASTTATKAFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEA"
BASE COUNT        12103 a         5093 c         5909 g         9936 t
ORIGIN      
        1 gtgatgtaaa tgattggtat aatgggtatt atggaaaaat attacccgga ggagatgtta
       61 tggatttttc caactttttt caaaacctca gtacgttaaa aattgtaacg agtatccttg
      121 atttactgat agtttggtat gtactttatc ttctcatcac ggtctttaag ggaactaaag
      181 cgatacaatt acttaaaggg atattagtaa ttgttattgg tcagcagata agtatgatat
      241 tgaacttgac tgcaacatct aaattattcg atatcgttat tcaatggggg gtattagctt
      301 taatagtaat attccaacca gaaattagac gtgcgttaga acaacttggt agaggtagct
      361 ttttaaaacg ctatacttct aatacgtata gtaaagatga agagaaattg attcaatcgg
      421 tttcaaaggc tgtgcaatat atggctaaaa gacgtatagg tgcattaatt gtctttgaaa
      481 aagaaacagg tcttcaagat tatattgaaa caggtattgc aatggattca aatatttcgc
      541 aagaactttt aattaatgtc tttataccta acacaccttt acatgatggt gcaatgatta
      601 ttcaaggcac taagattgca gcagcagcaa gttatttgcc attgtctgat agtcctaaga
      661 tatctaaaag tttgggtaca agacatagag ctgcggttgg tatttcagaa gtatctgatg
      721 catttaccgt tattgtatct gaagaaactg gtgatatttc ggtaacattt gatggaaaat
      781 tacgacgaga catttcaaac gaaatttttg aagagttgct tgctgaacat tggtttggca
      841 cacgctttca aaagaaaggt gtgaaataat atgctagaaa gtaaatgggg cttgagattt
      901 attgcctttc ttttggcatt gtttttcttt ttatctgtta acaatgtttt tggaaatatc
      961 tttaacactg gtaatcttgg tcaaaagtct agtaaaacga ttcaagatgt acccgttgaa
     1021 attctttata acactaaaga tttgcattta acaaaagcgc ctgaaacagt taatgtgact
     1081 atttcaggac cacaatcaaa gataataaaa attgaaaatc cagaagattt aagagtagtg
     1141 attgatttat caaatgcaaa agctggaaaa tatcaagaga agtatcaagt taaagggtta
     1201 gctgatgaca ttcattattc tgtaaaacct aaattagcaa atattacgct tgaaaacaaa
     1261 gtaactaaaa agatgacagt tcaacctgat gtaagtcaga gtgatattga tccactttat
     1321 aaaattacaa agcaagaagt ttcaccacaa acagttaaag taacaggtgg agaagaacaa
     1381 ttgaatgata tcgcttattt aaaagccact tttaaaacta ataaaaagat taatggtgac
     1441 acaaaagatg tcgcagaagt aacggctttt gataaaaaac tgaataaatt aaatgtatcg
     1501 attcaaccta atgaagtgaa tttacaagtt aaagtagagc cttttagcaa aaaggttaaa
     1561 gtaaatgtta aacagaaagg tagtttagca gatgataaag agttaagttc gattgattta
     1621 gaagataaag aaattgaaat cttcggtagt cgagatgact tacaaaatat aagcgaagtt
     1681 gatgcagaag tagatttaga tggtatttca gaatcaactg aaaagactgt aaaaatcaat
     1741 ttaccagaac atgtcactaa agcacaacca agtgaaacga aggcttatat aaatgtaaaa
     1801 taaatagcta aattaaagga gagtaaacaa tgggaaaata ttttggtaca gacggagtaa
     1861 gaggtgtcgc aaaccaagaa ctaacacctg aattggcatt taaattagga agatacggtg
     1921 gctatgtttt agcacataat aaaggtgaaa aacacccacg tgtacttgta ggtcgcgata
     1981 ctagagtttc aggtgaaatg ttagaatcag cattaatagc tggtttgatt tcaattggtg
     2041 cagaagtgat gcgattaggt attatttcaa caccaggtgt tgcatattta acacgcgata
     2101 tgggtgcaga gttaggtgta atgatttcag cctctcataa tccagttgca gataatggta
     2161 ttaaattctt tgaatcagat ggttttaaac tatcagatga acaagaaaat gaaattgaag
     2221 cattattgga tcaagaaaac ccagaattac caagaccagt tggcaatgat attgtacatt
     2281 attcagatta ctttgaaggg gcacaaaaat atttgagcta tttaaaatca acagtagatg
     2341 ttaactttga aggtttgaaa attgttttag atggtgcaaa tggttcaaca tcatcactag
     2401 cgccattctt atttggtgat ttagaagcag atactgaaac aattggatgt agtcctgatg
     2461 gatataatat caatgagaaa tgtggctcta cacatcctga aaaattagca gaaaaagttg
     2521 ttgaaactga aagtgatttt gggttagcat ttgacggcga tggagacaga atcatagcag
     2581 tagatgagaa tggtcaaatc gttgacggtg accaaattat gtttattatt ggtcaagaaa
     2641 tgcataaaaa tcaagaattg aataatgaca tgattgtttc tactgttatg agtaacttag
     2701 gtttttacaa agcgcttgaa caagaaggaa ttaaatctaa taaaactaaa gttggcgaca
     2761 gatatgtagt agaagaaatg cgtcgcggta attataactt aggcggagaa caatctggac
     2821 atatcgttat gatggattac aatacaactg gtgatggttt attaactggt attcaattag
     2881 cttcagtaat aaagatgact ggtaaatcac taagtgaatt agctgggcaa atgaaaaaat
     2941 atccacaatc attaattaac gtacgcgtaa cagataaata tcgtgttgaa gaaaatgttg
     3001 acgttaaaga agttatgact aaagtagaag tagaaatgaa tggagaaggt cgaattttag
     3061 taagaccttc tggaacagaa ccattagttc gtgtcatggt tgaagcagca actgatgaag
     3121 atgctgaaag atttgcacaa caaatagctg atgtggttca agataaaatg ggattagata
     3181 aataaatact gtattacaaa taagccgatg cgtatgcatc ggttttttgt gtttgtagaa
     3241 ataatttata gtacaaacgt aaaatgatat aaacaaaata aaaacaaagt aatcaatatg
     3301 taatataaaa tacactggta ctcaatatat aatgatgata aaattaattt taattagata
     3361 gagttgcttt gtgtttttaa cgcagatgct actacatatc ttaacagttg attaagtgac
     3421 atcatttaac agcgagaata atcaaccagg aggatgactt aatgaattta ttcagacaac
     3481 aaaaatttag tatcagaaaa tttaatgtcg gtattttttc agctttaatt gccactgtta
     3541 catttatatc tattaacccg acaacagcgt ctgcagcaga gcaaaatcag cctgcacaaa
     3601 atcaaccagc acaaccggct gaccccaatg cacagcctaa cgcaaatgct ggtgctcaag
     3661 ctaatcctac agcacagcct gctgcacctg ccaaccaagg tggtcaagca gacccagcag
     3721 caaatcaagg tggacaggct aatccagcag gaggcgcagt acaaccggca gcaggtacaa
     3781 cacaaccagc tggacaaggt aatcaagctg atccgaataa tgctgcacaa gcacaacctg
     3841 gaaatcaagc agcacctgca aatcaagcag gtcaaggaaa taaccaagca acacctaata
     3901 ataatgcaac accggcaaat caaacacagc cagcgaatgc tccagcagga gcgcaaccag
     3961 cagcagctgt agcagcaaac ggacaaactc aagatccaaa tgctagcaat actggtgaag
     4021 gcagtattaa tactacatta acatttgatg atccagccat atcaacagat gagaatagac
     4081 aggatccaac tgtaactgtt acagataaag taaatggtta ttcattaatt aacaatggta
     4141 agattggttt cgttaactca gaattaagac gaagcgatat gtttgataag aataaccctc
     4201 aaaactatca agctagaggg aatgtagctg cattaggtcg tgtgaatgca aatgattcta
     4261 cagatcatgg taactttaac ggtatttcaa aaactgtaaa tgtaaagcca gattcagaat
     4321 tgattattaa ctttactact atgcaaacga atagtaagca aggtgcaaca aatttagtta
     4381 ttaaagatgc taagaaaaat actgaattag caactgtaaa tgttgctaag actggtactg
     4441 cacatttatt taaagtgcca actgatgctg atcgcttaga tttacaattt attcctgaca
     4501 atacagcagt tgctgatgct tcaagaatta caacaaataa agatggttat aaatactatt
     4561 cattcattga taatgtaggt ctattctcag gatcacattt atatgtcaaa aatagagatt
     4621 tagcaccgaa agcaactaac aataaagaat atactattaa tactgaaatc ggtaacaatg
     4681 gtaattttgg tgcttcatta aaagcagatc aattcaaata cgaagtaaca ttaccacaag
     4741 gtgtaacgta cgttaatgat tcgttaacta ctacgttccc taatggtaac gaagacagta
     4801 cagtattgaa aaatatgact gtcaattatg atcaaactgc gaataaagta acatttacta
     4861 gccaaggtgt gacaacggct cgtggtacac acacgaaaga agttttattc ccagataaat
     4921 ctttaaaatt atcatataaa gttaatattg cgaatatcga tacacctaaa aatattgatt
     4981 ttaatgaaaa attaacatat cgtactgctt cagatgttgt aattaataat gcgcaaccag
     5041 aagttacact aactgcagat ccattttcag tagcggttga aatgaacaaa gatgcgttac
     5101 aacaacaagt aaactcacaa gttgataata gtcattacac aacagcatca attgcagaat
     5161 acaataaact taaacaacaa gcagacacta ttttaaatga agatgcgaat catgttgaaa
     5221 ctgcaaatcg tgcatctcaa gcggatattg atggtttagt aactaaatta caagctgcat
     5281 taattgataa tcaagcagca attgctgaat tagatgctaa agcacaagaa aagattacag
     5341 cagcacaaca aagtaaaaaa gttacgcaag atgaagtggc agcacttgta actaaaatta
     5401 acaatgataa aaataatgca atcgcagaaa ttaataaaca aacaactgcg caaggtgtca
     5461 caactgaaaa agataatggt atcgcagtgt tagaacagga tgtaattaca ccaactgtta
     5521 aaccacaagc gaaacaagat attatccaag cagttacaac tcgtaaacaa caaattaaaa
     5581 agtcaactgc atctcttcaa gatgaaaaag atgtagcaaa tgataaaatt ggtaaaattg
     5641 aaacaaaggc aattaaagat attgatgcag caactacgaa tgcgcaagta gaagctatta
     5701 aaacaaaagc aatcaatgat attaatcaaa ctgcacctgc aacaacagcc aaagcagaag
     5761 ctcttgaaga atttgatgaa gttgtacaag cgcaaattga tcaagcgcct ttaaatcctg
     5821 atacaacaaa tgaagaagta gcggaggcca ttgaacgtat taatgcagct aaagtttcag
     5881 gtgttaaagc gatagaagcg acaacgactg cacaagattt agaacgcgtt aaaaacgaag
     5941 aaatattcaa aattgagaat attacggact caactcaaac taaaatggat gcttataaag
     6001 aagttaaaca agctgctaca gctagaaaag ctcaaaatgc tacagtttca aatgctacag
     6061 atgaagaagt agcagaggca aatgcagcag tagatgcagc tcaaacagaa ggtttacatg
     6121 acatccaagt tgttaaatca caacaagaag tggctgatac taaagcaaaa gtattagata
     6181 aaatcaatgc tattcaaaca caatcaagag ttaaacctgc agcggatacg gaagtagaca
     6241 acgcatataa tacacgtaaa caagaaatcc aaaatagcaa tgcttcaact acagaagaaa
     6301 aagaagcggc atatacggaa ttagatgcta aaaaacaaga ggcaagaacg aatcttgatg
     6361 ctgcaaatac aaacagtgct gtaacaacag ctaaagacaa tggtattgct gcaattaatc
     6421 aagttcaagc ggcaacaact aagaaatcgg atgctaaagc agaaattgct caaaaagcaa
     6481 gtgaacgtaa aacagcaatc gaagcaatga atgattcgac tagtgaagaa caacaagcag
     6541 cgaaagacaa agttgatcaa gcagtagtta ctgcaaacgc tgatattgat aatgccgcag
     6601 caaacagtga tgtagataat gcaaaaacta caaatgaagc tacaatcgca gccattacac
     6661 ctgatgcaaa tgttaaacct caagcgaaac aagcaattgc agataaagta caagctcaag
     6721 aaacagcaat tgatgctaat aacggtgcaa taacagaaga aaaagcagct gcgaaacaac
     6781 aagttcaaac tgaaaaaaca acagctgata cagcaattga tggtgcacat tcaaatgcag
     6841 aagttgaagc ggctaaaaat gcagaaattg ctaaaattga agcaattcag ccagcaacaa
     6901 caacgaaaga taatgcgaaa caagcaattg ctacgaaagc gaatgaacgt aaaacagcaa
     6961 tcgctcaaac gcaagacatt acagcagaag aaattgcagc ggctaatgcg gacgtagata
     7021 atgctgtgat acaagcaaat agcaacattg aagctgctaa tagtcaaaat gatgtagacc
     7081 aagcgaaaac aactggtgaa actagtattg atcaagtgac accaactgtt aataaaaaag
     7141 caacagcgcg taatgaaatc actactacat taaacaacaa gttgcaagag attcaagcta
     7201 cgccagatgc aacagatgaa gaaaaacaag atgcagaaac tgaagctaat actgaaaatg
     7261 ctaaagcaaa tcaagctatc acagcagcta caacaaacgc tgaagtagat gaggcaaaaa
     7321 ctaatgcaga agcggcaatt aacgctgtaa caccaaaagt tatgaagaaa caagcagcga
     7381 aagatgagat cgaccaatta caagcagcac aaacagcggt tattaataac gatcaaaacg
     7441 caacaaatga agaaaaagaa gcagcgattc aacaactagc aacagcagtt acagacgcga
     7501 agaataacat tacagctgca actgacaata atggtgtaga tacagccaaa gatgcgggta
     7561 aaaattcgat tcaaagtaca caaccagcaa cagcagttaa atcgaatgca aaaaatgatg
     7621 ttgatcaagc tgtgacaact caaaatcaag caattgataa tactacaggt gcaacgactg
     7681 aagagaaaaa tgcagcaaaa gatttagttt ctaaagctaa agaaaaagca tatcaagata
     7741 ttttaaatgc gcaaacgact aacgatgtta cgcaattaaa agatcaagca gttgctgaca
     7801 ttcaaggtat tactgctgat acgacaatta aagatgttgc taaaggtgaa ttaacggcaa
     7861 aagcaaatga acaaaaagca cttattgcac aaactgcaga tgcgactacg gaagaaaaag
     7921 aacaagcaaa tcaacaagta gacgcacagt taacgcaagg taatcaaaat attgaaaatg
     7981 cacagtcaat tgatgacgta aacactgcaa aagataatgc agttcaagcc atcgacccaa
     8041 ttcaagcatc aacagatgtt aaaacgaatg caagagctga attgttaaat gaaatgcaaa
     8101 ataaaataac tgaaatcctt aatgacaata cgactaccaa tgaagaaaaa ggtaaagata
     8161 tagggccagt tagagcagca tatgaagaag gtttaaataa tattaatgtt gcaaattcaa
     8221 caggtgatgt aacaactgct aaagacgcag cagtacaaaa agttcaacaa cttcatgcaa
     8281 atcctgttaa gaaaccagca ggtaaaacag aattagatca agctgcagct gataagaaaa
     8341 cacaaattga acaaacgcca aatgcgtcac aacaagaaat taatgatgca aaacaagaag
     8401 ttgatacaga gttaaatcaa gcgaaaacaa atattgatca atcttcaact gatgactatg
     8461 tagatgctgc ggttagagat ggaaaagcta aaattaatgc agttaaaaca tttagtgaat
     8521 acaaaaaaga tgctttagct aaaattgcag atgcatataa tgctaaagtt aatgaagcgg
     8581 ataactctaa cgcatcgact tcaagtgaaa ttgctgaagc gaaacaaaaa cttgctgaat
     8641 tgaaacaaac tgcggatcaa aacgttaatc aagctacttc taaagatgac atagaagtac
     8701 aaattcataa tgacttagat aatattaacg attatacgat tccaacaggt aaaaaagaat
     8761 cggctacaac agatttatat gcctatgcag atcagaagaa aaataatatt tcagctgaca
     8821 ctaatgcaac acaagatgaa aaacaacaag caatcaagca agttgaccaa aatgttcaaa
     8881 ctgcgttaga aagcattaat aatggcgtgg ataatagtga tgttgatgat gcattaacac
     8941 aaggtaaagc gacaattgat gcagttcaag tagatgctac tgttaaagct aaagcaaacc
     9001 aagcgattga tgctaaagca caggaaacga aagaatctat tgaccaaagt gaccagttaa
     9061 ctgctgaaga aaaaactgaa gcattagcaa cgattaaaca aattacagat caagctaaac
     9121 aaggtattac tgatgcaaca acaactgccg aagttgaaaa agcgaaagct caaggacttg
     9181 aagcatttga taacattcaa atcgactcaa cagaaaaaca aaaagctatc gaagaattag
     9241 aaactgcact agaccagatt gaagcaggtg taaatgtcga ctctgatgct acaactgaag
     9301 aaaaagaagt atttacgaat gctttagaag agattttatc aaaagcaact gaagatattt
     9361 ctgatcaaac tacaaatgca gaaatcgcta ctgtcaaaaa tagtgcgctt gaacaactta
     9421 aagcacaacg tattaatcct gaagttaaga aaaatgcttt agaagcaatt agagaagtgg
     9481 ttaacaagca aatagaaata attaaaaatg cagatgcaga tgcatcggcg aaagaaattg
     9541 cacgtacgga tttaggtaga tattttgaca gatttgctga tgatttagat aaaacacaaa
     9601 caaatgcaga agttgcagaa ttacaaaatg taacgatacc tgcaattgaa gcgattgtgc
     9661 ctcagaatga tccggatgct aatcatagca gttcagataa taatgacgca acagcaaata
     9721 caaatgttaa tgcagagcca gacaatgctg gacaacctaa tgtaactgag tcaacagata
     9781 atgccaacgc agatacgtct tcaacaacga caaataatca taatgatgca gcaacaggtg
     9841 aaacaacagc aacatcaaca aacaattcat caactgatga tgcaaatgat aaaccgacag
     9901 ctaataacaa ttctagtgta gatgcatcaa tagacaattc ggcaacgggt aatggcacaa
     9961 ctgataaacc agcaacaact gagagcacag aaaaaacatc atctgctgat agcaaagata
    10021 atgcatctgt aaatgattca aatcaaaatg ctgaagtgaa taatagtgct gaatctcaac
    10081 caactaatgg caaggttgca caaccaaaat ctgaaaataa agctaaggta gaaaaagatg
    10141 gtagagattc aacaaatcaa agtatggtcg aatcaacaac tgaaacattg ccttcagcag
    10201 acataacaga accaaatgta ccttctaata catcaaaaga taaagaggaa agtacgtcaa
    10261 atcaaactga tgcaggacaa cttaagtctg aaactaacgt tgcatctaac gaagcagata
    10321 aatcgccaag caaagctgat actgaagttt cgaataaacc atcaacatct gcatcttcag
    10381 aagcaaaaga taaaatgact tcgactgagg atagccaaaa agctgacatg gcaacagcag
    10441 atacaaaaga taatcaagca tcaattggtt catctgcaga tgcaaacaat aaagctacgc
    10501 aaaacgacgg tgccaatgca tctccagcta cagtttcaaa tggaagcaat agtgctaatc
    10561 aagatatgct taatgtaact aagcctgaag aaaacaaagc aaatgcaaaa tcagaccaac
    10621 aaggaaaagt taataagcct aaacaacaag ctaaaacttt accagatact ggtatgtcac
    10681 ataatgacga tttaccatat gctgaattag ctctaggtgc aggtatggca ttcttaatta
    10741 gaagattcac taagaaagac caacaaactg aagaataaaa tttattcaag aatgtgaaca
    10801 tctagttatt tcatagggaa atattcctta ctaagtaacc aagtctaaca gttaatatga
    10861 atttgtttta gtaatgacca atagccattg aataggtggc tgtggctcga aaataaaaca
    10921 aattattaac gcaaaaatac tcctttcata cataacacaa gttaagtgtc tatgttgaag
    10981 ggagtatttt ttatgttcag aaatttaata ttgttaattc aacggaacta ctacattatt
    11041 actgattgtt aacgaataac gttggaaact tcttgtttca aacaaagctc aataaattat
    11101 gaaagtgaat tgtacgcttc aattatttca tcggctaagc catcgtcaac tttagcatat
    11161 tcgcttaaaa acgcttttaa accgtgttct ttaatgtaat tatttttctc aacagtttct
    11221 tggtctcctg tatcatcata cttcaataac aatgcagctg tatttaataa accagtgcgt
    11281 tctaaatctt tattataaag atatgttaat ggttttataa ttctatcttt agggccaatt
    11341 ttacgtaacg tacctcgtcc gacacgtgtc acttcatcag ataaataaga attgttgaaa
    11401 cggtctatta ttttttcaac ataaccagct tgttccgctt cagtaaaatc aaattcatta
    11461 gtaatatatt gacttgtttc agctaaaaca cggcgtaagc cagcttcaat tgaactatct
    11521 tttacagcgt ctaatacggt agctttacct gcaaatttac cagcatacgc taaatatgca
    11581 tgtcctgtat tcacagttaa taatttacgt tcaatatatg gtgttaaatc atcaacatat
    11641 ttaatatggt ttagttctgg accataccat gcatcttttt caacaaccca ttcgtaaaat
    11701 ggctcaacca taacgtctaa tatattttca ttcttttgta atggtacaat tctatcaact
    11761 gctgagttag caaaatgaat attgttacca agagggccag taatatcaag taccgctttt
    11821 ttcaatgtat cagttgccat aatggcattc tcacaagcaa caatattaac atggtttgtt
    11881 ttttctttta agaaagacgc aaaagattta gcaataatag gtagtatgtt aacaccaaca
    11941 gctgttgtaa taatatcagc ttctagaatt gcttgtttca acgcttcaga aggttgcatt
    12001 gaattaattg catcaacatt attcacgcgc gtcgttgttt tagactcatc agctaaaata
    12061 acatcgtatt gatgatcatg agctaaagca ttaatgattt cttcattgac gtctgcaaat
    12121 gttactttaa cattgttgtc tgcaagaata taaccaatga aaccacgacc tatgttacca
    12181 gcaccaaagt gaactgcttt cattatgcat ctgcctcctc gaatacttgt ttaatttctt
    12241 ctgcagattt tgcttgaacg atacgatcca cattttcttc ttcgctaaat gtaattgcaa
    12301 ttttagataa caagtctaaa tgttccccat ctttaccagc aatacccacg acaactttaa
    12361 ctacttcgcc atcccagtca acgccttcag ggatttgtaa taatgttaaa cctgagtgaa
    12421 tcacatttgt tttagcttca tctgtgccat gaggaattgc taagccattt cccataaatg
    12481 ttgatacaac ttgctcacga tctttcattg cttgaatata agcatctgtt acagcaccac
    12541 tatcaactaa ggctttacct gctttttcaa ttgcttcatt ttggctgtta acatttacat
    12601 ttaaaaagat attgtcatta ctaaataatt cgctcatttt gtacactcct aaataaattg
    12661 ttttttaatt cgttctaaaa atttgcttct taataatgtt tcaagttcct gtggattttc
    12721 cataaaagta tcaatagatt ctaatttcaa acttaaaaat tcggacaagt cacttaccag
    12781 tgatgccata aaatcattgt caggaataaa catggaaatc atatatttaa ttgtttgatt
    12841 gtcattttga atactaggca atgttaacgg ttcttctaaa actgtgatta gaatcatagg
    12901 gtgtttaatc atattgtctc ttaggtgtgg tattgcaaca ggatatggac ttaatatcca
    12961 tcctggattg tcaatcaatc gttgctctag tagttcagcg aatgacgttg gatcttttat
    13021 tgcatcgcat tgatatagat aatctgcgat atatgtttgc cactgtttaa cttcggtact
    13081 aactattttc actgaatcaa gcaaagttaa acccgaacgc atatgatgca ttaattgctc
    13141 tgatttttcg tcacttaaat gtttttcgaa aactgaagtt aagtttgaag tttgttcatt
    13201 ttcaaaagat gtgttgccat caatatcttt cgtttcaaca tgaacatcat ccttatcatt
    13261 catgtttgat gatttatcat gcgtctcttg gaacgtagac tttgtattta aaaactgtgc
    13321 gacataactg atatcactat ctggtaataa tgggtttacc attaaatagg gggtgtcgat
    13381 gtctaaattc acagtagaaa taatgccatc atattgactt aaatctagtg acttcaaatc
    13441 gctgactgat gcttgtgtaa tacgctcaat ctcactaaaa acttgctcta gacgagttga
    13501 taatagacga ctagttccca taccactgct gcaaacgact aatatgttta aaaatcgatt
    13561 accttggttt ttaatcgagc caccaaagtg taaaactata aaagcaattt cactctctgg
    13621 aaaaatcaaa tcaggccaac aatcatttaa agctaatctt acattttcaa atagtcttgg
    13681 atatttgaac ttaatcatgt ctgttaacgg attataggtc tcgatattag cttgcaaacg
    13741 attgattgca ggattaaggt gtagcgtcag tccatctact aaagtatcca aatctgcaaa
    13801 tgtcatacct gaaatcattt ctactttgtt aacaaacgct ttaattttgt tttcttcaca
    13861 acgattattt aatgatgtat cattaagatt ttttcgttta gctccacgta aatggatagt
    13921 aatgaaagca atttctgcct gattaaacgt gacgtcatat atttgatcaa gtttatcagc
    13981 aagttcgctt gctactttgt gttcaaatgt gttttgtaca gaatcataaa tatcatcgtt
    14041 taatgcgaca tactctccat tttttatacg tgaaatggag agcacgatat ggacagttaa
    14101 agttaaataa cttgattcgg taagttggta gggtaaggca cttaggtagt ccattaatag
    14161 tctttcaaca ttaaaaatac gctctaagtc aacaaagtct ttgtggattt gatttaatga
    14221 atgaaagaca aaatgatttt caattactga ataaacgcta gtactattta agttattcac
    14281 cattaattga cttaaaaatt cacgtttctt tgattcagta cctaccaagt aaataccttc
    14341 gccacgcttt cgagataaag atagttggta cttatttaaa tcgagctcta aatcatctaa
    14401 catctttgct aaagtctgga cagaaacgcc aatttcttgc gctaaactat attgtttaac
    14461 tggctccttg gcttgtatca aagcgtatat aataattact ttctgctctt caacagataa
    14521 gtcaatggtt tgaagttgtg cgatcgattg cttcaaatca tttaaatcag aatctgcgcc
    14581 agcaatgcgt agcccttttt tgtttgcacg ttctaaagtt aatgaaaatg aagttaaata
    14641 tgattcaaca ccttttaatt cacggtgaat agtacgagag gacaccgcaa gttgctgagc
    14701 aatgtcgtgt atagtgatat attgaccgtg gtacttaatc aatagggcta tcatttcttt
    14761 ttcacgtgta ctcaataaca tggcggtatc ctccattttt taatttattt aattattatg
    14821 cttgatcatc tttttttaga ttatttaaaa gttcttcata tcttggtgaa ttaaggaaat
    14881 tatccactga aatatggatg gcatttggtg tttgtttaat agcacgatca gttagttttt
    14941 tctgagtgat aactaattga gcatcttttg gcaattgatt aatcgcagta tttgtaactg
    15001 taatatcatt aatgcccgct tttttaaatt tattacgtaa catgcttgca cccattgcac
    15061 tagaacccat acccgcatcg caggcaaaaa taacatgatt tatattattg taattgtgtg
    15121 catcaacatc ttcagtgttg taattatcca atagcgcttc aggatcatca tctgatgaag
    15181 atgtttcact aacattacca ctagcatttt cttctgtatt aacattttta tcagaagata
    15241 ctaacttaga agcaacgctt gattttttcc ctttagtatt ttccatttga gctgtcgcag
    15301 cttctaaatc ctgctttggt tctctagtga acttcataat taaagtagct acaacgaatg
    15361 atacaagtgc agcaaggaac acaccgagta acatgtgcaa gaattcacct ctaggtgcat
    15421 ttaaacagta aactataaat gaacctggtg acgcgggact tttaaatcca aatcctgttg
    15481 cttgataagt tgcaacacca gtcattccac ctaaaataac agcgataaat aataaaggac
    15541 gcattaatac atatgggaaa taaatttcat gaataccacc taagaagtgg ataattccag
    15601 caccatatga cgttgctttt gcagtgcctt ttccaaaaat catataagca agtaagatac
    15661 ctaaacctgg tccagggtta gattcaattg tgtataaaat tgattgacca gcttttgcag
    15721 cttgatctgc accaagcggt gtgaatacac catggttaat cgcattgttt aaaaatacga
    15781 tttttgcagg ttctactaaa atacttacaa gtggaagtag atgtgcatgc actaatgatt
    15841 ctactgctac tgataaaata tgcatgataa acttcataat tggtgctaaa attttaaatc
    15901 ctgcaattgt catgataaat cctaaaatac cagctgaaaa attattaaat agcatttcaa
    15961 aaccttgtgg tgttctaggt tggattaatt ggtctgtctt tttcattaac caaccaacaa
    16021 gtggacccat aatcattgca ccaagtaaca ttggtgtatc aggtaatgca acgatgaccc
    16081 ccatagttgc tgttgctgcg atgataccac cacgtaaatc ataaattaaa cgaccaccac
    16141 taaatgcgat caataatggg attaaataag taatcattgg tcctgctaaa gtagctaaat
    16201 ctttgttagg taaccatcca ttatctataa aaatggccgc gataaaaccc caagcgatga
    16261 aagcgccaat gtttggcatg atcatactac ttaagaatga tccaaatgct tgaacacgac
    16321 gaccaattcc ttttttctct tcagtttgtg acatatttac acctcttttt taatatatgt
    16381 gactatatat agtgtataga gacgttactt gttactcaat ataaacaaaa atcaactttg
    16441 tcaaaataaa tgtgacaaaa ttaaataaag tgtcatcaat gtgacagtat agatattttg
    16501 aaaaagtaaa acaaaaaaat tgttttagga tttttaaaat tttattgtga aaatatttgc
    16561 aaaacaaaac aacaccgttg tacaataatg attaatggaa agggggaaag ttcggcagta
    16621 cagttaaagc gcctgtgcaa ataaatattt gtatttgaag attaaaggtt aatatatgag
    16681 tggcctttat agagtgcaat atatgtattt gtagacgagg aggatagtga tcgaatagat
    16741 cggcggatgc tatcccggat gtggctcatt cgttagctta ttaagtaaaa cattagggtg
    16801 acttaatgga caaagttaat aagatcgcca gaaattgaat ataaaaaata ttaatatgga
    16861 aagtacagtg tgagcaattt gtatagttgt aaaaataact atgcttaatt tgttatggat
    16921 gaatgcgatg atagcatgtt cctatttata ttatgaaagc agattgtcaa tctaaattat
    16981 cggcaataaa tcataattta cgcgtactat tccaatatgg aggaaaatgt cgttatgtgt
    17041 ggaattgttg gttacattgg ctatgataat gccaaagaat tattattaaa aggtttagaa
    17101 aaattagaat acagaggtta tgactctgca ggtatcgcag tagtaaatga tgataataca
    17161 actgtattta aagaaaaagg tcgtattgca gaattacgta aagttgctga tagtagcgat
    17221 tttgatggac ctgttggaat cggtcacaca cgttgggcaa cacacggtgt accgaatcat
    17281 gaaaactctc atccacatca atcatcaaat ggccgtttta ctctagttca taacggtgtt
    17341 attgaaaact atgaagagtt aaaaggtgaa tacttacaag gtgtatcatt catttcagaa
    17401 acagatacag aagttatcgt tcaattagtt gaatactttt caaatcaagg actttcaact
    17461 gaagaagcat ttacaaaagt tgtgtcatta ttacatggtt catatgcatt aggtttatta
    17521 gatgctgaag acaaagacac aatctatgtt gctaaaaata aatcaccatt attattaggt
    17581 gttggtgaag gtttcaatgt tatcgcatca gacgcacttg caatgttaca agtgacaagc
    17641 gaatataaag aaatccatga ccatgaaatc gttattgtta aaaaagatga agttattatt
    17701 aaagatgcag atggaaacgt tgtagaacgt gattcatata ttgctgaaat tgatgcatca
    17761 gatgctgaaa aaggtgttta tgcacactac atgttaaaag aaattcatga acaaccagca
    17821 gtaatgcgtc gtattattca agaatatcaa gatgcagaag gtaacttgaa aattgatcaa
    17881 gacatcatca atgatgttaa agaagcagac cgcatttacg ttattgcagc aggtacaagc
    17941 taccatgcag gtttagtagg taaagaattt ttagaaaaat gggctggcgt accaactgaa
    18001 gtacacgttg catcagagtt tgtctacaac atgccattat tatctgaaaa accattgttc
    18061 gtttatattt ctcaatcagg tgaaactgca gatagccgcg ccgtattagt tgaaactaat
    18121 aaattaggtc ataaatcatt aacaatcact aatgttgcag gttcaacttt atcacgtgaa
    18181 gcagaccaca cattgttatt acacgcgggt cctgaaatcg cagttgcatc tacaaaagca
    18241 tatactgcac aaattgcagt attatcaatc ttgtctcaaa tcgttgcaaa agagcacggt
    18301 cgtgaagcag atattgattt attgagagaa ttagcaaaag taacaacagc aatagaagca
    18361 attgttgacg atgcaccaat tatggaacaa attgctacag atttcttaga aacaacacgc
    18421 aatgcattct ttatcggacg tactattgac tataacgtaa gtttagaagg tgcgttaaaa
    18481 cttaaagaaa tttcttacat tcaagcagaa ggttttgctg gtggagaact taaacatggt
    18541 acaattgcct taatcgaaga tggcacacca gttgttgctt tagcaactca agaaaacgtt
    18601 aatttatcaa ttcgtggtaa cgttaaagaa gtagtagcac gtggtgcaca tccatgtatc
    18661 atttctatgg aaggtcttga aaaagaaggc gacacttatg tcattccaca tgtacatgaa
    18721 ttattgacac cattagtttc agtagttgca ttacaattga tttcatatta tgcagcatta
    18781 cacagagatt tagatgtgga taaaccacgt aaccttgcta aatctgttac tgtggaataa
    18841 ttcacttttt tagaatcaat catgtattaa aattaaagta tatggcaccc ttttagatta
    18901 atcgactaga agggtgcttt tttaggtcga cttagctttt acttcatctt aatttggcag
    18961 aaatgcgtaa aaatgaagtg ttttatttat ttaaatagtc tgacaattaa gggtgttatg
    19021 ttaatatgat tttatgagaa gtatggagta gcaataaagg ggtgacctcg catgttaatt
    19081 caattagatc aaattgggcg aatgaagcaa ggaaaaacaa ttttaaaaaa tatttcgtgg
    19141 caaattgcta aaggtgacaa atggattcta tatggtttaa atggtgctgg taagacaacg
    19201 ctattaaata tattaaatgc atatgagcct gcgacaactg gcggtgttaa tctatttggt
    19261 gaaatgccag gcaaggtagg gtattctgca gagactgtac gacaacatat aggttttgta
    19321 tctcatagtt tactggaaaa gtttcaagat ggtgaaagag taatcgatgt ggtgataagc
    19381 ggtgctttta aatcaattgg tgtttatcaa gatattgatg atgaggtacg taatgaagca
    19441 catcaattac ttaaattagt tggaatatct gctaaagcgc aacaatatat tggttattta
    19501 tctacaggtg aaaaacaacg agtgatgatt gcacgagctt taatggggca acctcaggtt
    19561 ttaattttag atgagccagc agctggttta gactttattg ctcgtgagtc actattgaat
    19621 atacttgact cattgtcaga ttcatatcca acgcttgcga tgatttatgt gacgcacttt
    19681 attgaagaaa ttactggtaa ctttactaaa attttgctat taaaagatgg ggaaagtgta
    19741 caacaaggat taatagatga tattttgaca tctgaaaaca tgtcacgatt tttccagaaa
    19801 aatgtagcag ttcaaagatg gaataatcga ttttctatgg caatgttaga gtaaatattt
    19861 tgcaaataat aagtaataat gacaaaattt aattaagata aaatggacag tggagggcaa
    19921 tatggataac gtaaaagcaa tatttttgga catggatgga acaattttac atgaaaataa
    19981 tcaagcatca acgtatacga aagatgtcat taatcgattg agagagaaag gatataaagt
    20041 atttttggca acaggacgtt cgcattctga aatacatcaa cttgtacctc aagattttgc
    20101 ggttaatggc atcattagtt caaatggaac aattggagaa gtagatggag aaattatctt
    20161 caaacatggt ttatcattgg ctcaagtgca acaaattatt aatttagcta agcgccaaca
    20221 aatttattat gaggtatttc cttttgaagg taatagagtt tctttaaaag aagatgaaac
    20281 atggatgcga gatatgattc gtagtcaaga tcctattaat ggcgtaagtc atagtgaatg
    20341 gtcttcaaga caagatgcgc ttgctggtaa gatagattgg gtaactaagt ttcctgaagg
    20401 tgaatattca aaaatttatc tattcagttc taatttagaa aaaataacag catttagaga
    20461 tgaattaaag caaaatcatg tgcaactaca gattagtgtt tcaaattcat caagatttaa
    20521 tgcggaaaca atggcttatc aaactgataa aggcactggt attaaagaaa tgattgcaca
    20581 ctttggcatt catcaagatg aaactttagt tattggagat agtgataacg acagagcaat
    20641 gtttgaattt ggtcattata cagttgctat gaaaaatgca cgccctgaaa tccaagcatt
    20701 aacttcagat gtaacggcat acacgaatga agaggatggc gcagcaaaat atttagcaaa
    20761 acatttttta gctgattaat tggtgtttta ataataaatc caaaatagtt gatgagcaat
    20821 atacaaagag cagtaaagtt attttctgtt agaaaatgcc ttactgctct tttgtatgct
    20881 agtaatccag gtactagtgt tatttcttct ttttgtcttt cttctccata tatggaatga
    20941 agaaaatcac aaatagggca cagagtatag ctacaattga accgaatgtc atagttatat
    21001 ccgcttttat atggtttatg catttaagta tactataaca actaccgttt ctttaagtag
    21061 agtgtttgcg ctaagggcat gcataaggga ccctaacaac tccaaactcg catttgcaat
    21121 tttgagatca tcaaacattt cattccataa gtaagatatg tttcacttta tatgagtttt
    21181 ttacattcac attctgtagt ggttgtaaga aaattttttc tttctcttag gcagtaatag
    21241 gcaatttacc tgtactagta tgaacttagt agttaaatcg ttcattaaga taaaaaaact
    21301 taggaacttt ttccaaatac aattcgtctt gataagcatg aatttcatct aatgatttcg
    21361 ttattgattc aactttacct tttttctagg gcggccggaa atacaaggtt ttactttaaa
    21421 attaaaacca tcagcgaatt atttaaatct acgatttatt cgaccatttg tacgttcttt
    21481 tcttggctta tacatgactg ttttcaagtt atccgttaca agttcattat gtacaccttc
    21541 tattagctca aacgattgtg ttaataaatt aaataaggca tcacttgatt tacgcattgt
    21601 catgcatgat tataaaacga gaataagaaa gtagtagcac tcctatattt agtgaaatca
    21661 tttgattatc tttcttttta aaattaatgg tttctttcca gtcaaactgt acttgatgac
    21721 tgggttttgt ctcgaatctc gttgaaactt taggcgacat ctgttgataa cttttcataa
    21781 aataacgatt aaattcatca tgcttaagga tagctctaaa tgtagaatag gcacaggtta
    21841 atccatgttt aaccttcaaa tattgccaaa atatatgctt atagtagaac tattgtttac
    21901 tagtgtctga aagtatcttt tggatgatcg gataataatt gtcaataata gactgacgat
    21961 ttctctttcg aaatttttat ttttcaatgt ctactttgaa gggagcttat cagccgaaaa
    22021 agcgggcgcc cctctttacc agagcataaa aagtgagaaa ttaaattttt atatgatgaa
    22081 caaaagatag caggggaaga aatagctagt cagacagggg tatgtcgctc aactgtttat
    22141 cgagttgtta acgaaatgaa aaaacttata ctatgaatta ctgtttaaaa gcgtgcatgt
    22201 tataatattt attgagcaag ttggatagat ggtggctaat ctcttaataa aggggtgatg
    22261 cctatggtta tagttgttac tcctagaaag gactagcatg tctgattttg aaatgctgat
    22321 ggttgtatta acaatcattg gtttagtatt gattagtaat caaggccata aaaaataacc
    22381 ttctattcgc tttgaccggc atttttgaag gctattttta aataaaatat aaggtcaccg
    22441 tctttttaac gggctcatta gggtaacatg tttctgagtg ttgccctttt tgtgtttcaa
    22501 gagttaaaga ttttcatctt ttacttctac ttactacaaa tatattttaa cacatttttc
    22561 ttatgaattg tagttctgaa cataatcaga aataataaag ccaactatca atacaacaaa
    22621 aaacaataaa aagtatagaa tgtaatcacg gactatataa aaagtactgc agacctcttc
    22681 atatattttt tcggcatgcc atgaaattag catcatgtta tttcggtatc agctactaaa
    22741 gttgaaaaat agaggaacac agttggatgc tgcgcatcaa ctgcataaga gcccctaaca
    22801 actaaagttg aaaaatagag gaatacagtt ggatgctacg catcaactgc ataagagccc
    22861 ctaacaacta aagttgtaag gggctctaaa atttattttg gttgattgtc ttctggttta
    22921 tctgaagtca tcgtttttgt tgtattatca tttaatgaat cttgtgcttt ttctgttttt
    22981 gtttcaacag atgttgccac tttatcattt tctacttttg tattttttgg ttcagtgaca
    23041 acttttttat ccttgtcagc tgtttttact ttatctttag taattttttg accacgtttt
    23101 aagtaatatt gaacgatacc cattaaaatg attgcatgaa taacaacgaa taaaataatt
    23161 gtcattactg tataaactct tatgatattt tctgcaacac tttgagagag tgattgcgta
    23221 ttaaatgaaa tcaagtaacc aacaggtgtt ttaaataaaa taataattaa gtttaataat
    23281 agtattccaa tgaaaaattt gaaaaatgtt ttttgaccat ttttcattgc tttaaatcca
    23341 ttagctaagt gtgtttttac tttatcgtta gttgattcaa cgaaactagt cataaagttg
    23401 aaaataggta ttaacaataa ccaagtaata attgctttaa tcaataaaac aacaatgatt
    23461 atgatgcttt gagttgtaat agcaatacca atcaggtgtg tactatctgc atatgatgat
    23521 tgtactgaat tcataatcat ttctgatact ggagaaaata atttgtatag taatgatgtc
    23581 agaagtgaaa gaacgataat cattgctata gatacaagac cgatcagaac gctctttaaa
    23641 taattacctt ttttaaatgc aataaataaa tcggtaaatt ttactttttc atgactcatt
    23701 gctcgtttca taacatttgt aattccgata aaaatttgaa ctaaaacaaa cagtgaaact
    23761 acagctgcta taagtaatat agcgataact tttaaatatg catctactgg tggtttttgc
    23821 ccaaattgtg aataaattgc aatagtttgt acatacttag caagctggaa attagcaagt
    23881 aaatatacaa ctgcaattac agcaaatgct ataagtgcat atatcaatgt tattaataat
    23941 tgtggtttag catttttaaa tgctgattta aagtaagtaa acaaagtggt gcctcctttt
    24001 cttcatataa atacaataac aaaaaacacg ttattttcat attaatatct aataaaaatt
    24061 aattaaagtg actaaagtta gagaattata atagtaaatt gtcaaaaata tgactaatgt
    24121 aagaaataat agttgaaaat ttgttaataa acattgcgtc atcaaagacc aaacaagtcg
    24181 ccataaagac ataaaaaaac tactgccatt gaaaagttta atcgatgcaa tggcagtagg
    24241 ttaaaattat taatgtacat gtgcgtgatg atgagcatga tggttatgtg aatgactatg
    24301 tgttcctgaa catataatcg attcatcatg tttgtgatta ggcgtttcta attgaatagt
    24361 catatggtga atatttaaat gtaataaatc atgctcaatg ttttctaata ataattcaca
    24421 ttctttcatt gtcaatgtat ggtctacaac aacatgacaa ctcaatgcat tcatatcatt
    24481 tgaaattgtc caaacatggc aatcatgcac actttgtatt cgtgaatcct ttttaatagt
    24541 agttataact tcatctatat caacatcact tggtgtgcct tccattaaaa tgttaattga
    24601 agattttgtg ataccccaag cactttttaa aataataacg gaaactaaaa tacttgcgat
    24661 agggtcggcg attgtccatc caaatgccca aattaaaata gctgcagtaa tggcgccaac
    24721 tgaacctaat aagtctccga taacatgtag aaaagcacca cgcatattta aattgtgtga
    24781 agtgtcgccg cctttaaaca taaagaatgc aacaacgata ttgacaatta aaccgataat
    24841 actaatgatt aacatttctt ttgattgaac ttcagaagga acaaagaaac gtttaatcgc
    24901 ttcaaaaaca atcaaaatac ttattacaaa aagcgttaca ccgttaaata acgctgcgag
    24961 tacttcgaaa cgtttataac caaatgtttt tgtagttgtg gcattctttt cagcataaat
    25021 aaatgcgaca agtgcaacac ctaatgagaa tgtgtcgcta aacatatgga taccgtcaga
    25081 tagtaatgcc aagctgttag caaggagacc gccgatgatt tcgataaaca tatatagacc
    25141 gattattaaa aacgatataa acaatacttt cttattattt gtagttacat gactatgcat
    25201 atggtcatga tgatgatgtg aatgagacat attataaccc actttcttta ggatgattcg
    25261 cgtgatgtat ggcttgcttt aacatagttg ctacgtggat gtcatctaat gaataaatca
    25321 ttgattggcc ttgtcgtttt gctttcacaa gatgcacact tttaagtaat tttaattggt
    25381 gcgagacatt tgattgagat aaattcaatt gatgtgaaat gtgaccaaca cttgcttcac
    25441 tgactgataa caattccata atacgtattc gattgtaatc gcctaatgcc ttgaaaattt
    25501 cagttacgcg ttctaatgta tctgtatgta tttctgaata ttgttctgac attatatgac
    25561 aatcctttca tatgaatatt tgttcatata ttaatttaat acgttctcag tagtatgtca
    25621 attgattttt atagttgtaa caagttttac atatctcata aagtggtatg gcatagggaa
    25681 taataaatag gagagtgttg taaatgaata ttttggttat aggtgctaat ggcggtgtag
    25741 gttcgttact agtgcaacaa ttagcaaaag aaaatgtacc atttactgct ggtgttaggc
    25801 aatccgatca acttaatgcg ttaaaatcac aaggtatgaa agcaattctt gttgatgttg
    25861 aaaatgattc aatagagact ttaactgaga cgtttaaacc attcgataaa gttatctttt
    25921 cagtaggttc tggagggaac acgggagcag ataaaacaat tattgtcgat ttagatggtg
    25981 ctgttaaatc aatgattgcg agtaaagagg ctaatattaa acactatgtc atggtttcaa
    26041 catatgattc gagacgtcag gcttttgatg atagcggtga tttaaagcca tatacaatag
    26101 ctaaacatta cgctgatgac tatttaagac gttcaggttt gaattataca attttacatc
    26161 caggggcact tacaaatgca gcaggatctg gaaagataga agctgcacaa tattttgatg
    26221 gtaaaggtga aattcctaga gaagatgttg cgacagtatt aaaagaaatt gtcacatcca
    26281 atcattttaa ttaccaagag tttcaaatta ttagtggtga gcaggacatt aaagatgctt
    26341 taactcaatt tgaaattgaa aaggattaat aaggatagta aatatgattt gaagcgggtt
    26401 acgttttcgt aactcgttat ttttttgaaa atgatgaatt ataagtatga ataagtacgt
    26461 attgtctatg cattttacaa agaatttgaa caatgtatgg cacaactatt aaagttgttt
    26521 attaattggt ataatgggcg agtaaattta aagggaaatg tgtacaaatg taagagagag
    26581 ataatctcta tgctattaat ttttaaaaat gttaaagtac tgtaaattta agtgaagcgc
    26641 tttaattggc agtactgcaa tgttagtaaa tatggtacaa tcactaatag ttaatttgaa
    26701 atgtggaaat ttataatacg atgtaaatga gaacaaaatc aaaatataat ggaacaacaa
    26761 catcagagtt ctattgctta cttaatcaca atggaggtgc aacaatgcca ttatttttac
    26821 aaccaatttt aaaaacaaaa ttatggggcg gtcaacgtct aagtgagttt ggatatcaat
    26881 tagacaatga tacaactggg gaatgttggt gtgtgtcagc acatccaaat ggtacaagcg
    26941 agattattaa tggaccatat caaggtcaaa cattagaccg tatttggtca gaacatcgtg
    27001 aattgtttgg tgatttccca agcaaagatt ttccgcttct aactaaaata gtggatgcaa
    27061 gagaatcact ttctattcat gtgcaccctg ataattctta tgcttatgag catgaaaacg
    27121 ggcaatatgg caaatctgaa tgttggtata ttatagatgc tgaagaagat gcagaaatag
    27181 ttatagggac attagcagag tctagagaag aagttgcgaa tcatgttcaa cacggaacga
    27241 tagagtcgat acttagatat attaaagtaa aacctggaga attctatttt attccagcag
    27301 gaacagtaca tactatttct tcaggaatac tagcatacga aacgatgcaa tcgtcagaca
    27361 ttacatatag actttatgat ttcaatcgtc aagataatca atataatgat agaccgttaa
    27421 atattgaaaa agctttagac gttattcagt acaatgcccc attacctaat attttgcctg
    27481 aaagtgaaat tattgaaaac cataagtgta cacacattgt atcgaatgat ttctttacat
    27541 tggttaaatg ggaaatttct ggcacgttaa attatatgaa gcctagagag ttctgtttag
    27601 ttacagtgtt ggaaggtgaa gggcaaatga ttgtcgatgg tgaaattttc aaactgacta
    27661 ctggtacaaa ctttattttg acttctgaag atttggatag tgtctttgaa ggtgatttca
    27721 cattgatgat tagctatgtg taatagtgta ataacaattt aggagtgaat gatttgaagc
    27781 aatttctata tattgcgtta gtatgtggtg tgatagcagg tcttggtgct ttcttacata
    27841 taccgcagta tccgagcatg acaattccac gtatagtagc tattttagga attatcagtg
    27901 ctatgttgac ttttaaagac aagcaaatca gcgcctcatt aaagtttagc gcattgttaa
    27961 ttaatgtgct gccattatgc ggtacctttg tagcttcaaa ttaattgtcg atagcgcgtt
    28021 tgaatataat acagtgcatt aacagaagaa atatgattgt gttaatgaca ttgatgaaaa
    28081 taaagcacat caacataacc tctcacttgc atagtagtga gggggttttt gttggtttat
    28141 ataaaatgaa gtattttata tttaatgaaa attaagacat gctaaaattc taacggcagt
    28201 atagatattt aaaaatgtgt atcaatggcg ctatagagat aagcaagcat agtggtatca
    28261 tatataaatt tgtaattttt attgcatagt gtatccaagc atgtttttta tatgcaaaat
    28321 aggtagctaa atatgttaaa ctgtttgtaa caatttttat cattaagaca gatataataa
    28381 ataaaatata gaaaactgat taaaaggtat acagtcgctc gtatgtgtgt gtccaactgc
    28441 atatagagtc gcacttgttg taaagcaaaa tagttcgact tacttaaaag ggaaaacgtt
    28501 aagggtctgg gacgtaaatt aatgtcctgg gcgctacagt gttataggaa atgcgattaa
    28561 aatcgcagtg aacaaagggt atacagttga tcgcatacgc gtccaactgc ataagaaaca
    28621 ctaaaagaat gtaattcata gtgtttctaa aatttattca ggttaaggag tattaagatg
    28681 acagcagaaa cgaattattt ttggttgaat tgtggatata accgttggaa tcataatgaa
    28741 ccgttagtag gacaaacagc tttatttgaa tcaggcgcac attttaaccc atctcaagga
    28801 tttagagcgt ttaaaaaggc taaagtagga gatcaagtca tcttttatca agttcagacg
    28861 gatactggac tacttggatg tggtgaaatt attagtgttg aaacaggtgc acaaaataaa
    28921 atacgtgtac agtttcgttt taatgaacaa ttgaaaccat taacagcaga ttatctaaag
    28981 cgtagtgaag ctttggaatt tcgtatgagt aatatgaaag agacattgtt caaccaaatc
    29041 actgcagaag agtttgattt aataagtggt cttggtaaag gcgaaattaa gataccacgt
    29101 tatttctttt tagctgaaac tgaagaattt gaacctggaa accaatatac gatttatacg
    29161 cacacgtaca acggtattaa acgtaatggt tatcattttt atactcagtt agaagaaggc
    29221 gacaatatta ttttttataa tcgaacaaaa aatcaatctg tcgttggaat tggagaagta
    29281 tcaaagcata ttcatgaaaa accaccaatt cctggtagaa caaatagtac agtgattgaa
    29341 gtgtcttatg agaaagacat tacgccgatt acattaagta cattaaataa gcatcctaaa
    29401 ttgaaaaact tatatttcct tcaagaaaat gcaaaacagg ctattgcaag tatgtcacaa
    29461 gcacagtatg atgcaattat tgaaatgagc gataataatg gattgaaatc tccatttgaa
    29521 atggttcaaa aaccagatat gcttgaaagt gaaaaagaag aagcgttaaa gccatttatt
    29581 ttacttgttg tagatcgaaa agaagaaggc ttaaaagcgg ctaatgactt attgcaaaaa
    29641 gcgaatgcaa atcctgttat aacaacaggt catcctgatt tttcagaaga tatgctttat
    29701 ggcaagtatt taccaaatga aacaggggca ttatactatc gtgaaggatt tattactcag
    29761 ttaatgccta aaaaggataa aagttattta gttattgata attttaatcg tatagataca
    29821 gacattttcc aaacgtatat taatgtgtta gaaggctatg aggtaacatt accacgttat
    29881 aataaagacg gtaatatgat taaatggtca cgacaaaaag attctttcta ttatttcaat
    29941 cctaattggc atattgtagg tattacatat gatagtttag aggaaattaa ggagaaatat
    30001 tcagagcagt ttttaaaata cacacgtata gtcaaagtca aacatgacta atgatatatg
    30061 gtagtttttg aaaagaagta aggaaagatt aaaaattgca taattgcata tttgaataac
    30121 cgtataaaga aaaaatgacg ggtaatcaac cataagagct aattaagagc ttatgataga
    30181 ttactcgttt ttattttaat aaatgataaa gacagtatat gaatataaat gaatttatgt
    30241 tactgaatta aaactttata tgagtttcca gtggtatgtt gaataaggcg aacgactaaa
    30301 cgctaagtta ataataagaa ataagattac actgtagatt gagaaattta aagtaaataa
    30361 aaaatacatt atacaataaa tataaaactg aaaaataaat tttatggcaa tttcgtgtcg
    30421 cgaggactta tcaagtgaca accttgttta aatgttttta aattaggaaa aatgactata
    30481 agtactattg aaggagcgaa taaattatgc caatcgtata ttatgatgga aactgtatat
    30541 attgttataa ctatgtcatt tggttaattc aacatgacct accaagaaat tatcgatttg
    30601 ctacgttaaa aggtgaagtt ggacaacaat tttttaaaca acatccagaa gcggcaaata
    30661 aaaatagtgt gattttacag aaaggtgatc gtatatattt tgaatcgcag gcgattattc
    30721 aactaatcac agcactacct aatcgaacta aattattggg cgtaggttta tggcttgtac
    30781 ctaaaccagt ccgtaatttc ggttatcgaa tgtttgctaa taatagaaat agaatgtgga
    30841 aaacaacatg gcaccaacca aatgattatg aaaaatcgtt ctttttagat gataatgcga
    30901 aagtaaaact tactgattga taaaacatag ttgctaattg ataatagaca tactagatga
    30961 tggattaaaa tttaggcatt taaaaagcca aacacctcac aattaaatta gaataattat
    31021 aaaaaagaga gcgaaaacac tttacagatt agaatcatta taatataata attaatataa
    31081 acaagtaaga cgtagacaat tttaaggagt gtattaaata tgagtaatca acaagatgtt
    31141 gtaaaagaat tgaatcaaca agtagcaaac tggacagtag catacacaaa gctacataat
    31201 ttccactggt atgtgaaagg gcctaacttc ttctcattac acgttaaatt tgaagaatta
    31261 tataatgaag caagccaata tgtagacgaa ttagctgaaa gaattttagc ggtaggagga
    31321 aaccctgtag gtacattaac tgaatgctta gagcaatcaa ttgttaaaga agctgcgaaa
    31381 ggttactctg cagaacaaat ggttgaagaa ttatctcaag atttcacaaa tatctcaaaa
    31441 caattagaaa aagcgattga aattgctggt aatgctggcg atgatgtatc agaagatatg
    31501 tttataggta tgcaaacatc agtagataaa cataattgga tgtttaaatc ttacttaagc
    31561 taatctacag ataagtaata atataaataa aagccaacac ataaatgggg aatacgccca
    31621 gaatgtgttg gctttttgag tttaatctca ttgttaaaga gcaattaaag tcaatttaaa
    31681 taagttactc tgcgatttct aaagcaattt ccatcatttg tgtaaatgaa ttttgacgtt
    31741 cttcaggtgt agtagcttcg tcacgtaaaa tatgatcact tactgtgaaa ataccaagtg
    31801 cttttttacc agcatgaatc gcatttaaat ataaaccagc tgattccatt tcgataccta
    31861 aaatacccat ttttttccaa gcagcattga atgttggatc ggcattgtaa aatgtatcag
    31921 aagataatac gttacctacg tgtgtagtag caccgatttg gtcagcgaca tttttagctt
    31981 tagttactaa ctcgaagtca gcgataggcg cgaaatgacc tggaatattg tattgatcta
    32041 cataatttga attagttgat gcagcttgtg caataataac atcgtataag ttaacatttt
    32101 cttgtaatgc gccacaagaa ccgatacgaa tgattgtgtc tacatcaaag aagttgtata
    32161 actcatatga gtaaataccg atacttggaa tacccatacc agaacccatt acagaaactt
    32221 ctttaccttt atatgtacca gtgtaaccaa acatgttacg tacatcgtta aattgttcaa
    32281 cattttctaa aaaattatca gcaatatatt ttgcgcgtag cggatcgcca ggcattaata
    32341 ctgttttagc aatttttact ccatttggtt gaatatgtgg tgtacctttt gtcatttgtt
    32401 atcttctcct taatattcat taataatttt aagataacat ttgtttccct tttttgcgaa
    32461 tttttgtact atagaattaa agaattataa aaatataatc ctgattagct tgttgtcaag
    32521 tcatcgttca taatgaagat atcacgttca attgtattgt ttgtttatgg gaaatgaatt
    32581 aatgtaatag tatatgtatg cggttacata aaaagcgaac atctaacctg atatttaaat
    32641 gaacctgacg ctcaatcaac taatttacaa ccgtattttt ataatcaacc ataaaggagg
    32701 agatagaaaa tgaatagtgc aaaattgatt gatcacactt cattgaagcc tgagtcaaca
    32761 cgtacgcaaa tcgatcaaat catcgatgaa gcgaaagcat acaattttaa atctgtatgt
    32821 gtgaatccaa cgcatgttaa atatgcagca gagcgactag ctgattcaga ggtgttagtt
    32881 tgtacggtaa taggattccc attaggtgcg tcgacaactg caacgaaagc atttgaaaca
    32941 gaagatgcaa ttcaaaatgg tgcagacgaa attgacatgg tcatcaacat cggcgcatta
    33001 aaagatgggc gttttgatga tgtacaacaa gacattgaag c
//
LOCUS       DAHHOJ010000018        32854 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.018, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000018 DAHHOJ010000000
VERSION     DAHHOJ010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32854)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32854)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32854
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.018"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..647
                     /locus_tag="NBH32_000722"
     CDS             <1..647
                     /locus_tag="NBH32_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium transporter CorA"
                     /protein_id="HCD1514349.1"
                     /translation="VTYHHQTLESLNKVVYKYMNTLDAELDCADVVILILDMMVDKYF
                     NFVYALEDSVYHFEDRHVDDRFNKMVMDSVFKLRSDLIKVKRVLFPMQELIDTMKQNG
                     DLIIDNKHSLYIQHIDDHLIKQRNIIRTAQEMTNEIRENFESYTSFRMNSIMQVLTLV
                     SVIFSPLTFIAGIYGMNFVNMPALHLHYGYYICLAVMFVIAVVLIIFFRRKKWF"
     gene            678..1727
                     /locus_tag="NBH32_000723"
     CDS             678..1727
                     /locus_tag="NBH32_000723"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCD1514350.1"
                     /translation="MSDFQREQRKNEHVEIAMAQSDAMHSDFDKMRFVHHSIPSINVN
                     DIDLTSQTPDLTMAYPVYINAMTGGSEWTKNINEKLAVVARETGLAMAVGSTHAALRN
                     PRMAETFTIARKMNPEGMIFSNVGADVPVEKALEAVELLEAQALQIHVNSPQELVMPE
                     GNREFVTWLDNIASIVSRVSVPVIIKEVGFGMSKELMHDLQQIGVKYVDVSGKGGTNF
                     VDIENERRANKDMDYLSSWGQSTVESLLETTAYQSEISVFASGGLRTPLDAIKSLALG
                     AKATGMSRPFLNQVENNGIAHTVAYVESFIEHMKSIMTMLDAKNIDDLTQKQIVFSPE
                     ILSWIEQRNLNIHRG"
     gene            complement(1775..2983)
                     /gene="gltS"
                     /locus_tag="NBH32_000724"
     CDS             complement(1775..2983)
                     /gene="gltS"
                     /locus_tag="NBH32_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCD1514351.1"
                     /translation="MIELNAITTLCLACILYLLGKAIVNHVNFLKRICIPAPVIGGLI
                     FAILVATLDSFGMVKIKLDASFIQDFFMLAFFTTIGLGASLKLFKLGGKVLLLYFMFC
                     AIISVIQNIVGVSLAKVLNIKPLLGLTAGSMSMEGGHGNAAAYGKTIQDLGIDSALTA
                     ALAAATLGLVFGGLIGGPVVKFLIKRYNLKPQHSDDTFKDYSQVAYNEHLHSKFNATE
                     VFFIQFTIVVFCMAVGSYFSHLFTSQTGINVPIYVGSLFVAVIVRNISESFNFNIVDL
                     KITNQIGDVALGIFLSLALMSIQLIEIYKLAIPLIIIVLVQVVVMILFAVLILFRGLG
                     KDYDAAVMVGGFIGHGLGATPNAMANLDVITKKYGNSPKAYLVVPIVGAFLIDLIGVI
                     VIMGFIQWFS"
     gene            complement(3320..3928)
                     /locus_tag="NBH32_000725"
     CDS             complement(3320..3928)
                     /locus_tag="NBH32_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HCD1514352.1"
                     /translation="MDFVNNDTRQIAKNLLGVKVIYQDTTQTYTGYIVETEAYLGLND
                     RAAHGYGGKITPKVTSLYKRGGTIYAHVMHTHLLINFVTRSEGIPEGVLIRAIEPEEG
                     LSAMFRNRGKKGYEVTNGPGKWTKAFNIPRAIDGATLNDCRLSIDTKNRKYPKDIIAS
                     PRIGIPNKGDWTHKSLRYTVKGNPFVSRMRKSDCMFPEDTWK"
     gene            4327..4947
                     /locus_tag="NBH32_000726"
     CDS             4327..4947
                     /locus_tag="NBH32_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCD1514353.1"
                     /translation="MEQLPQVGFMKSFVLFWKNYVNFKGRSRRSEYWYMMLWHLIFMV
                     PAVCVLIISLFLFFISIAADNETLIVMSILMIILTSVYTFVYSLATFIPNLALCVRRF
                     HDISRTMLFPMIMTAFSIIFYIVLQIIDSYYDSDFTLMPMGLNILLVFLYFVYLSLTI
                     VMIVFLCFDSKPANKYGESPKYPSSIKNQPVTSETPKTPGETDNQL"
     gene            5179..6078
                     /locus_tag="NBH32_000727"
     CDS             5179..6078
                     /locus_tag="NBH32_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514354.1"
                     /translation="MGLVIEHVTKRFGKMTAVNDISLELESGKMLGFLGRNGAGKTTT
                     FRMILGLSEPTEGHITYNGKKLDKTMYNRIGYLPEERGLHGKLTVEEELKYLATLKGM
                     SKTEIQQQISYWLERFDITENRKKRIDSLSKGNQQKIQLLASMLHKPELLILDEPFSG
                     LDPVNVELLKEAVKDLNDWGSTIVYSSHRMEHVEELCDDVCILDKGQLVVSGDINHVR
                     ASNGNKKVVIESETTLPDLTNIRGIIHSENMKQGLQLTIENEDVAKDIYQVVAHQGYV
                     KRFQVVEPSLQDIFIEKVGGKDA"
     gene            6071..7303
                     /locus_tag="NBH32_000728"
     CDS             6071..7303
                     /locus_tag="NBH32_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1514355.1"
                     /translation="MHKFWATFTLTYIKKIKAKSFVIFMIIIAALMIGLSNIDKIINM
                     FDDGPDKIGVAAPNEQIYKVFKQQANTFHSDAKFTKVSIEDAEKEVKKHKLDKAYIIK
                     VNQNRTLQGTIISEKRVSHEDSQKVQALLTAIQTNMVAGELNINKEDLQKLQAQSKVD
                     NKVISNDEVDKVSEGQKIFNYALAYGIIFLMFFIVLNYASQIAMEIASEKTSRVIEMI
                     ITSISPIQHIFAKILGVIAVAVTQIILIVLMAVICIFAFDLKDLLQGFNVEMNQLSWQ
                     IIIVGIISSIVGIMAYVLLAAILGSLTSRIEDLNQSLMPLTLLGMIAFYIAIFTINNP
                     DGQLALITSYIPFLAPFQLVVRAQTSGLQIHEVILSSLISIVMVAVLIWIAIKTYKDS
                     VLTFERGLFNSMKRVFKK"
     gene            7287..7472
                     /locus_tag="NBH32_000729"
     CDS             7287..7472
                     /locus_tag="NBH32_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514356.1"
                     /translation="MYLKNKIDESDTHLSYKMMMKACYSYFFIFSYRIMSKKVYNMNV
                     KTNFLKWECEVRRNVNK"
     gene            7521..7847
                     /locus_tag="NBH32_000730"
     CDS             7521..7847
                     /locus_tag="NBH32_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCD1514357.1"
                     /translation="MLYPIFIFILAGLCEIGGGYLIWLWLREGQSSLVGLIGGAILML
                     YGVIATFQSFPSFGRVYAAYGGVFIIMSLIFAMVVDKQMPDKYDVIGAIICIVGVLVM
                     LLPSRA"
     gene            7877..8896
                     /locus_tag="NBH32_000731"
     CDS             7877..8896
                     /locus_tag="NBH32_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HCD1514358.1"
                     /translation="MIVEVEHQRQGVDLIKIDNDETKIIFTNYGARVVSWKYHDNNIV
                     LGNVVEADEFYHSNPFKFGASIGRYSGRIDNAKFKLKGKEYQLEKNNGEHHLHGGCHG
                     LDNKLFDYEIRNEIAQIKVIFKTVLKSADDHFPGDIDVTITHIYDADHQWSIEYEAVA
                     SEDTLFSPTNHVYFNLNRDNNVVDNHRISSNQLDMYVLDERNIVTGDILDLHEVFEDN
                     KIKLSDIFTSQHAQLSQQMIRFGGLDHPFTVGEHKMYVENHEFMLEVDTDMPHVVFFT
                     FNQPDEWDSPFNIYKPHSGFTLETQFLPNDINLYGDKAPSILKAGEVFNSKTSFRIEE
                     KVEND"
     gene            complement(9130..9783)
                     /locus_tag="NBH32_000732"
     CDS             complement(9130..9783)
                     /locus_tag="NBH32_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HCD1514359.1"
                     /translation="MISIHAISTGKIQDLPYSSKRPMRSALDKTKISQTTWLSSTGFT
                     GDEQAYKDHGGPHKAVCGFSKHNYALYQDDLPSLPTHAMFGENLTFDYLDESDVYFGN
                     QYRLGDALIEVSEIREPCWKIQAKYNIPDLVKRMSTSGKTGFYFRVLKQGYVSPNDQL
                     YLIQEAPIEHRLSVQQLNDLYYNDRQNQDLLRYALNNPFLSPTRRDKLQKMYNRTLK"
     gene            9957..10643
                     /locus_tag="NBH32_000733"
     CDS             9957..10643
                     /locus_tag="NBH32_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase A"
                     /protein_id="HCD1514360.1"
                     /translation="MKDVKALKLMTLNDVLSQINGDMTLGIGTGSTMELLLPQMAQLI
                     KERGYNITGVCTSNKIAFLAKELGIKICEINDVDHIDLAIDGADEVDPSLNIIKGGGG
                     ALFREKVIDEMASRFVVVVDETKMVQYLGEMFKLPVEVDKFNWYHILRKIESYADIKV
                     ERRVNEDVAFITDNGNYILDCKLPKGIDPYKFHEYLIHLTGVFETGYFLDMADQVIVG
                     TQEGVKILEK"
     gene            complement(10709..11965)
                     /gene="sdpC"
                     /locus_tag="NBH32_000734"
     CDS             complement(10709..11965)
                     /gene="sdpC"
                     /locus_tag="NBH32_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpC"
                     /protein_id="HCD1514361.1"
                     /translation="MKNNKISGFQWAMTIFVFFVITMALSIMLRDFQSIIGVKHFIFE
                     VTDLAPLIAAIICILVFKYKKVQLAGLKFSISLKVIERLLLALILPLVILIIGMYSFN
                     TFADSFILLQSTGLSVPITHILIGHILMAFVVEFGFRSYLQNIVETKMNTFFASIVVG
                     LMYSVFSANTTYGTEFAAYNFLYTFSFSMILGELIRATKGRTIYIATTFHASMTFGLI
                     FLFSEEIGDLFSIKVIAISTAIVAVGYIGLSLIIRGIAYLTTRRNLEELEPNNYLDHV
                     NDDKETNHTEAEKSSSNIKNAEKTGVATASTVGIAKNDTENTVADEPSIHEGTEKTEA
                     QHHIDNQTESNHDEDHDITSESVESAEPVKHAPQSDDLTNDSNEDEKQSLKEPATYKE
                     DRRSSVVIDAEKHIEKTEEQSSDKNK"
     gene            12255..13190
                     /locus_tag="NBH32_000735"
     CDS             12255..13190
                     /locus_tag="NBH32_000735"
                     /EC_number="3.5.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HCD1514362.1"
                     /translation="MYKQGEPNLWTGRLDSETDPKKFRHFQTVTFEDLSKLEKSSMPS
                     GVGILGYAVDKGVALNKGRIGAKEGPDAIKQAFAGLPDLNQCETLVDYGNVYHDHEEL
                     IDTQKEFAMLAAKSIANHRQTFLLGGGHDIAYAQYLATRKVYPTQSIGVINIDAHFDT
                     RAEQQSTSGTSFRQILEEDENTDYLVLGIAQGGNTQSLFDYAKEKKIDYVFADELLSH
                     VSPTIKDMIERFVHEHDVIMFTICMDVIDSAFAPGVSAPAVLGLYPHTVLELAKRIIP
                     SDKVSSVSIAEMNPTYDADNRTAKLVANLVHHFLK"
     gene            complement(14102..14986)
                     /locus_tag="NBH32_000736"
     CDS             complement(14102..14986)
                     /locus_tag="NBH32_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1514363.1"
                     /translation="MKIIQLEYFLAIVKYNSFTKAAQFLHISQPSLTATIKKMEADLG
                     YDLFTRSTKDIKITEKGIQFYRYASELVQQYRSTMEKMYDLSVTSEPRIKIGTLESTN
                     QWIANLIRKHHSDYPEQQYRLYEIHDKHQSIEQLLNFNIHLAITNEKITHEDIRSIPL
                     YEESYILLAPKETFKNQNWVDVENLPLILPNKNSQVRKHLDDYFNRRNIRPNVVVETD
                     RFESAVGFVHLGLGYAIIPRFYYQSFHTSNLEYKKIRPNLGRKIYINYHKKRKHSEQV
                     HTFVQQCQDYLYGLLEAL"
     gene            15146..16807
                     /gene="hutU"
                     /locus_tag="NBH32_000737"
     CDS             15146..16807
                     /gene="hutU"
                     /locus_tag="NBH32_000737"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HCD1514364.1"
                     /translation="MRKIQAKKGLSIECKGWEQEAVLRMLYNNLDPEVAERPEDLVVY
                     GGIGKAARNWEAFEAIEKTLRELESDETMLVQSGKPVAVFKTHEEAPRVLISNSVLVP
                     EWANWDHFNELDKKGLIMYGQMTAGSWIYIGSQGIVQGTYETFAELGNQHFNGDLAGT
                     VTLTAGLGGMGGAQPLAITMNHGVAICVDVDETRVDKRIDTKYCDVKTADLDEALKLA
                     EEAKERGEGLSIGLVGNAVDIHQAILEKGFKIDIITDQTSAHDPLNGYVPQGYSVEEA
                     KVLREKDPKKYVELSQASMAKHVELMLEFQKRGAVAFDYGNNIRQVAFNNGVKNAFDF
                     PGFVPAYIRPLFCEGKGPFRFAALSGDPKDIERADEEMRKLFPENEKLLRWLDLAEEK
                     ISYQGLPSRIAWLGYGERAKMGLALNRLVRDGEISAPIVIGRDHLDAGSVASPNRETE
                     SMKDGSDAVGDWAVLNALINTAAGGSWISFHHGGGVGMGYSLHAGMVVVADGSERAER
                     RLERVLTTDPGMGVARHVDAGYDIAIQTAKEKGIHIPMIDKAGDK"
     gene            16807..18045
                     /locus_tag="NBH32_000738"
     CDS             16807..18045
                     /locus_tag="NBH32_000738"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HCD1514365.1"
                     /translation="MNDLIINHIAELILPKSTDKPLKGKELDELNVVKNGTVVIKDGK
                     IVYAGTHTDDYDATETIDASGKVVSPALVDAHTHLTFGGSREHEMSLKRQGKSYLEIL
                     EMGGGILSTVNATRETSEDDLFKKAEHDLLTMIKHGVLAVESKSGYGLDRENELKQLK
                     VSNRLAEKYDLDMKHTFLGPHAVPKEASSNEAFLEEMIALLPEVKQYADFADIFCETG
                     VFTIEQSQHYMQKAKEAGFKVKIHADEIDPLGGLELAIDEQAISADHLVASSDKGKEK
                     LRNSDTVAVLLPATTFYLGKEDYADARGMLDNNGAIALATDYNPGSSVTNNLQLVMAI
                     AALKLKLSPNEVWNAVTVNAAKAIDINAGTINTGDKANLVIWDAPNHEYIPYHFGINH
                     AEKVIKNGKVIVDNTLSFKA"
     gene            18320..19441
                     /locus_tag="NBH32_000739"
     CDS             18320..19441
                     /locus_tag="NBH32_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 peptidase aminoacylase family protein"
                     /protein_id="HCD1514366.1"
                     /translation="MGLKDQAVAWRRYFHQFPELSDKEFKTTQKIKDILTEHHIRILD
                     LPLATGLVAEVGQGPSCIAVRADIDALPIQELVEQDFKSKNEGVMHACGHDIHMASIL
                     ATAVKLKEIEGTLTGRVKFIFQPAEELGHGAFKIIETHALKDVQAVLGFHNDPSRSVG
                     TFAIKTGAITSAVDRFEFHIKGVGGHAAKPEQCNDPVIVLAQLINSIQSIVSRNLSAF
                     DEAVVTIGQISCGNTWNVIADHAYVQGTVRSFDPVVRKLVETRLQNIADGLAQAYNMK
                     INLNYTHLPGAVMNDEVLTHKAIAVAQHVGYKMEMMEQPLTIGEDFSGYSQHFPSVFA
                     LIGSHSEYDLHHPQYKPDERILEKVPEYFVEFVKRLLHE"
     gene            19550..20431
                     /locus_tag="NBH32_000740"
     CDS             19550..20431
                     /locus_tag="NBH32_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1514367.1"
                     /translation="MAAQDPRTKFKTTDYEKQEQEVPGLQSEMTPAPDCGETSYQGHQ
                     RLEGYKMLVTGGDSAIGRAAAIAYAKEGADVAINYLPSEEQDAQEVRQVIEESGQKAV
                     LIPGDIRDEQFNYDLVEHAYQQLGGLDNVTLVAGHQQYHDDIHGFTTEAFTETFETNV
                     YPLFWTVQKALEYLKPGASITTTSSVQGYNPSPILHDYAASKAAIISLTKSFSEELGP
                     KGIRVNCVAPGPFWSPLQISGGQPQSKIPTFGQKTPLGRAGQPVELCGTYVLLASEES
                     SYTTGQVFGVSGGVQID"
     gene            20918..21490
                     /locus_tag="NBH32_000741"
     CDS             20918..21490
                     /locus_tag="NBH32_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514368.1"
                     /translation="MALIVSRAILFLILVLAFGIATVFPEQKLPVYILFTLFVVVAIV
                     SNVWLTHQNVKKHIIKFDKRVAYFLGVLSGVFYIGIVIYGSTIIGYYNFISFIIFAIS
                     IVLVQILENILGINFKNTLPSNKQLKVISKQRVQQFKVISLILVSVSIVFIFIALMLK
                     WWLLLVISIIVLIIITVAMIVYEKYYTTPD"
     regulatory      21543..21638
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            21715..23028
                     /locus_tag="NBH32_000742"
     CDS             21715..23028
                     /locus_tag="NBH32_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC family protein"
                     /protein_id="HCD1514369.1"
                     /translation="MEVHKKSNAWALFPLLLFVALFLGVGIITGDFTSMPLNVAITIT
                     VIVALLMNRKESFAKKVEVFTKGAGHSNIILMMLIFILAGAFSNTAEKMGGVKSTVNL
                     GLSLIPENLIIVGLFVICMFVSISMGTSVGTVAAIAPVGYGFAQATDVPTALAMATVV
                     GGAMFGDNLSMISDTTIAAVRTQHTKMKDKFRVNFKIVLPGAILTIIILYFLTNGISL
                     NHAKNYDYNLIKVVPYILVLVLALLGVNVIIVLIGGTLLAGIIGLIDGSFGWMGLLDA
                     VSKGIISMEDIAMIALLIGGLVGIIQHNGGIEWLLQFVRSKVKSKRGAELGIASLVSV
                     ADIATANNTISIIMSGPLAKNIADEYDVDSRKSASILDIFGGCFQGLLPYSPQVISAA
                     GVAGISPLMLFPYSIYPILLGVCGLVAILFNFPKLKKNTSQEMKH"
     gene            23195..23701
                     /locus_tag="NBH32_000743"
     CDS             23195..23701
                     /locus_tag="NBH32_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD1514370.1"
                     /translation="MAKFNVENEHVEVEIEKLYKFSPELVYEAWTKKDLLKQWFMTSA
                     RTNKEIEADVKEGGKYRIVDQQRNGKVNVIEGIYESLVMDEYVKMTIGMPGLSETQDV
                     IEVEFFERETGGTQMLFYYRSLVEKERRFTNLEYKQKKKEYHDAMVHGFELMFDKMYH
                     VIETSTQQ"
     gene            complement(23759..24523)
                     /locus_tag="NBH32_000744"
     CDS             complement(23759..24523)
                     /locus_tag="NBH32_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1514371.1"
                     /translation="MFLDEHINRNFDKLNDNDLHIAHFINTHIDECKNMKIQDLAQFT
                     HASNATIHRFTRKLGFDGYSDFKSYLKFESNKTHQLPSNSIDSFKQEIDSTFSYLERV
                     DYQLLTEKMHTASTIYLYGTGRAQLNVASEAQRILLTLHNHVIVLHDEHELKMILNKS
                     EPNDLFFIISLSGETHELLEVTNLLQLRQKYFISVTTMKDNTLAQKANYNVYVSSNTF
                     YLTDGTDYSSFISYHIFFETLVRKYNEYLLHNDISN"
     gene            24853..26457
                     /locus_tag="NBH32_000745"
     CDS             24853..26457
                     /locus_tag="NBH32_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1514372.1"
                     /translation="MNAIKRFGSAMIVPVLMFAFFGIVLGFATLFKNPTIMGSLADQH
                     TFWFKFWSVIESGGWVIFTHMEVVFVVGLPLSLAKKAPGHAALAALMGYLMFNTFINA
                     ILTQWPHTFGANLEKGVENVPGLKSIAGIATLDTNILGGIIISAIITWIHNRYYSKRL
                     PEMVGVFQGLTFVVTISFFVMLPLAAITCVIWPTVQHGIGSMQHFIIASGYIGVWLYH
                     FLERVLIPTGLHHFIYAPIEVGPVVVNHGLKAEWLQHLNEFAKSTKPLKEQFPYGFML
                     QGNGKVFGCLGIALAMYATTPKENRKKVAALLIPATLTAVVVGITEPLEFTFLFIAPY
                     LFVLHAVLAASMDTLMYAFGVVGNMGGGLLDFISTNWLPLGKEHWGTYVAQVIIGLIF
                     VAIYFFLFRFLILKFDIPLPGRKKTEEEVKLFSKQDYKNKKGDSVDSKRASTGNEYED
                     KAAYYLDGLGGKENIKDVTNCTTRLRLTVYDESKVADTEYFTHQQMAHGLVKSGKSIQ
                     VVVGMTVPQVREAFEQMVEDQSSEDK"
     gene            complement(26523..27053)
                     /locus_tag="NBH32_000746"
     CDS             complement(26523..27053)
                     /locus_tag="NBH32_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514373.1"
                     /translation="MLIDKFETYIINIAGLNDRTTRKKLSKLCKSVQFCDALQFSINK
                     QFNQYVLEISLPKQQLPYFISFLSFHQYSIFQVLSPKKINELLDSDNLYQSAKRFDIN
                     IDGLQDAFIKDKVIDIMNMFQNHTDITYTLNKSHAHIICTPEIFAKLLHTIATRNIDI
                     LSANYRSSSMSKARIS"
     gene            27321..28238
                     /locus_tag="NBH32_000747"
     CDS             27321..28238
                     /locus_tag="NBH32_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HCD1514374.1"
                     /translation="MFQKLSIFATKSFLVWMLVAAVIGFIFPQHVATLGKWVPYLLGI
                     VMLGMGLTITPNDFKMVFKAPRAVIIGVCLQFSIMPTLAFIIAKSFHLPPDIAVGVIL
                     VGCCPGGTSSNVMSYLAKANVALSVSITTVSTLLAPFVTPALIYLFANEWLEVSFLSM
                     LWSVVQVVLIPIALGIVLQIINRKIAEKASTALPIISVVAISLILAIVVGGSKHQILT
                     TGLLIFLVVILHNVLGYTIGYWLARLLKLDRQDQKAVSIEVGMQNSGLAVSLAALHFN
                     PIAAVPGAVFSFIHNITGPILAKYWSKKL"
     gene            28313..28948
                     /locus_tag="NBH32_000748"
     CDS             28313..28948
                     /locus_tag="NBH32_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1514375.1"
                     /translation="MYRAVIFDFDGTIIDTEQHLFNVINKHLEMHNADPISIDFYRSS
                     IGGAATDLHDHLIKAIGSENKDKLYEEHHLTSTTLPMIDTIKSLMAFLKQRHIPMAIA
                     TSSVKAEIMPTFKALGLDDYIEVVVGREDVEQVKPDPELYLSAVQQLNYMPTQCLAIE
                     DSVNGATAAIAAGLDVIVNTNKMTSAQDFSNVDYVAKDIDYDQIVARFFTK"
     gene            28959..29150
                     /locus_tag="NBH32_000749"
     CDS             28959..29150
                     /locus_tag="NBH32_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514376.1"
                     /translation="MMGYIILFFLAGPVILGVGNLVIGPIFNKQTPFHVQVRSFVVGS
                     MIYLILATIDYFLLLQGKL"
     gene            complement(29246..29431)
                     /locus_tag="NBH32_000750"
     CDS             complement(29246..29431)
                     /locus_tag="NBH32_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514377.1"
                     /translation="MTTALIGVIVLILLIISFVPNYRAMKSAKEQGSNATRATIMVGI
                     DIILIVLIIVTLLLKYM"
     gene            complement(29428..29784)
                     /locus_tag="NBH32_000751"
     CDS             complement(29428..29784)
                     /locus_tag="NBH32_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514378.1"
                     /translation="MLKQILPRATKISILFAVAFFIINYIGMEKPDILYLVGRTIIAT
                     LVFILICLTLFTIINSPERKIKLGTTLPIALIIGIIFGAIFLTVQIGVITGLIIGVIA
                     TFIWELIEKNKGGRSS"
     gene            30064..31443
                     /locus_tag="NBH32_000752"
     CDS             30064..31443
                     /locus_tag="NBH32_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1514379.1"
                     /translation="MENNELQRGLSARQIQMIALGGTIGVGLFMGATSTIKWTGPSVI
                     LAYLIAGIFLFLIMRAMGEMIYLNPTTGSFATFASDYIHPAAGYMTAWSNIFQWIVVG
                     MSEVIAVGEYMKFWFPELPTWIPGIIAILLLMAANLFSVKAFGEFEFWFALIKVVTII
                     LMIIAGFGLIFFGFGNGGHAVGISNLWTNGGFMPNGIVGFFFALSIVIGSYQGVELIG
                     ITAGETKDPQKNIVKAVNGVIWRILIFYLGAIFVIVSVYPWNQLGDIGSPFVATFAKI
                     GITFAAGLINFVVLTAAMSGCNSGIFSASRMIYTLAHKGEMPKIFTKIMRNGVPLYTV
                     VAVSLGILIGALLNVILPLYIDGAKSIFVYVYSASILPGMIPWFMILFSHLRFRKLHP
                     EEVEGHPFKMPGGAVTNYLTILFLILVLVGMVFNVETRISVLIGVIFLTIVTIYYFIR
                     YNKNNVKAK"
     gene            complement(31832..32704)
                     /locus_tag="NBH32_000753"
     CDS             complement(31832..32704)
                     /locus_tag="NBH32_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1514380.1"
                     /translation="MSNVLTEIDSQYPYMTKNEKKIAKFILNSPQKVIKMRSQDLASL
                     LDISTSSVIRFSKKITDGGFHDLKINISKYVPKASSIYNVELMNNESTESLRTKLHTR
                     TTRALNHANNELNDKTIDQICHCLKRSETIFIYGFGASFVVATDLYQKLSRIGLNIQL
                     VQETHIFATLLATHNSNDSVILITNNGTQSEMQSMVKVIDDYHIPIITITSTRDNPVA
                     QASNIVLTYGKTDENEMHMGATTSLFAQMFTIDILYYRYVALNYHASLDFITQSKMAL
                     DNYRKHLSNINFKH"
BASE COUNT        11081 a         4599 c         6325 g        10849 t
ORIGIN      
        1 tagtaacata tcatcatcaa acgttggaat cattaaataa agtagtctat aagtatatga
       61 atacattaga tgcagaatta gattgtgcag atgtcgtcat attaatttta gatatgatgg
      121 ttgataaata tttcaacttt gtatatgcac ttgaagatag tgtttatcat tttgaagacc
      181 gccatgtaga tgatcgcttt aataaaatgg tcatggatag tgtctttaag ttacgttcgg
      241 atttgattaa agtaaaacgt gtactgtttc ctatgcaaga gctaattgat acaatgaaac
      301 aaaatggtga tttgattata gataataaac attcgctata cattcaacat atcgatgacc
      361 acttgattaa gcaacgtaat atcattcgca ctgctcaaga aatgacaaac gaaatacgag
      421 aaaattttga gtcatacacg tcatttagaa tgaatagtat tatgcaagta ctgacattag
      481 tatcagttat attttcaccg ttaacattca tagctgggat ttatggtatg aactttgtaa
      541 atatgccggc attacattta cattatggat attatatttg cttagctgta atgtttgtta
      601 tagctgtcgt attaatcatc tttttcagac gtaaaaaatg gttttaaatt agaaatgacg
      661 aaaaggtagg tttaatcatg agtgattttc aaagagaaca gagaaaaaat gaacatgttg
      721 aaatagcaat ggctcaatct gacgcaatgc attcagattt tgataagatg cgttttgtgc
      781 atcattctat cccatcaatt aatgtaaatg atatcgattt gacatcacag acgcctgact
      841 taacgatggc atatccggtt tatattaatg caatgacggg tggtagcgag tggacgaaaa
      901 acatcaatga aaagctagct gtagttgcaa gagaaactgg cttagcgatg gcagttggat
      961 caacacatgc agcattgaga aatccacgca tggctgagac gtttacgatt gcgcgaaaaa
     1021 tgaatcctga aggcatgatt tttagcaatg ttggtgcgga cgtaccagta gaaaaggctt
     1081 tggaagcagt tgaattactt gaggcacaag cgttacaaat ccatgttaat tctcctcaag
     1141 aattagttat gcctgaaggg aatcgtgaat ttgtgacttg gttagataat atagcgtcga
     1201 ttgtatcacg agtgtctgtt ccagtcatta taaaagaagt tggatttggt atgagcaaag
     1261 aattaatgca tgacttacaa caaataggcg tcaagtatgt cgatgttagt ggcaaaggtg
     1321 gtactaactt tgtagatatt gaaaatgaac gtcgtgcaaa taaagatatg gattacttat
     1381 catcatgggg acagtctaca gttgagtcat tacttgaaac aacggcttat caaagcgaaa
     1441 tttcagtttt cgcgagtggt ggtttacgta caccactcga tgcaattaaa agtttagcac
     1501 ttggtgcaaa ggcaactggt atgtcacgtc cgtttttaaa tcaagttgaa aataatggca
     1561 ttgcacatac agtagcttat gtagaatcat ttattgaaca catgaaatca ataatgacga
     1621 tgttagatgc taaaaatatt gacgacttaa cacaaaaaca aatcgtattt agtccggaaa
     1681 tattgtcatg gatagaacaa cgtaacttga atatacatcg aggataaata aaagaggagg
     1741 cgaaggcctc ctcttttatt tatgagtttg gtgtttaact aaaccattga atgaatccca
     1801 taatgactat aacaccaatt aaatcgatta agaatgcacc aacaataggt acaactaaat
     1861 atgctttagg tgagtttcca tattttttag taataacatc taaatttgcc atggcatttg
     1921 gcgttgcacc aagcccatgc ccgataaaac cacctaccat tactgcagca tcataatctt
     1981 ttcctaaacc tctaaataaa attaaaacag caaataaaat cataacgaca acttgaacta
     2041 aaacgataat aataagaggt atagcaagtt tataaatttc gattaattga atgctcatta
     2101 gcgcaagaga taagaaaata cctaatgcga catcaccaat ttgattagta atttttaaat
     2161 ctacaatatt aaaattaaaa ctttcagaga tatttcggac aataacagct acaaataatg
     2221 agccaacgta aattggaaca ttaatccctg tttgagatgt aaacaaatga ctgaaataac
     2281 ttccaactgc catacagaat acaacgattg taaattgaat gaagaatact tcagtggcat
     2341 taaatttact atgtaaatgt tcgttatatg ctacttggct ataatcttta aatgtatcgt
     2401 cactatgttg aggctttaag ttataacgtt tgattaaaaa ttttacaaca ggaccaccga
     2461 taagccctcc aaatacaaga cctaaagttg cagctgcaag agccgctgtc agtgctgagt
     2521 caatgcctaa atcttgaatt gtcttaccat aagcagcagc attaccatga ccgccttcca
     2581 tagacatgga acctgctgtt aatcctaaca aaggtttaat atttaatact tttgctagtg
     2641 atacaccaac tatgttttga atgactgaaa tgatagcaca aaacataaag tataatagca
     2701 agactttgcc acctaattta aataatttca atgatgcacc aagaccgatt gtcgtaaaga
     2761 atgctaacat gaagaaatct tgaatgaatg aagcatctaa tttaatctta accatgccaa
     2821 atgaatccaa agtcgcaact aaaatagcaa agattaagcc gccaatcaca ggtgctggta
     2881 tacaaatgcg ttttaaaaaa ttaacgtgat taacgatagc tttaccaagt aagtaaagta
     2941 tacaggctaa acataatgtt gtaattgcat taagttctat cattttcaaa tcccccttgt
     3001 tgaatatccc tctccctaaa taagctttta tatgtaagcg ttataaaagg gcacttttaa
     3061 cattatacat gcaacaaatc aatatttcta gtttatgaaa ataatatcaa taatattaat
     3121 gcatctaaat ttaataatta taaataattc tattgttata ttttataatt aagaacaagt
     3181 tcaaaaagag gagatgaatc aaaaaatatg atggacaaaa agaaaaacaa tcaacccatc
     3241 cgcatgttga cggtacgatt gattgactta taaggagata gatttcattt tcatgatagt
     3301 aatcaatgaa agatggcatt tatttccaag tatcttcggg aaacatacaa tctgatttac
     3361 gcatgcgaga cacaaatgga ttacctttca ctgtgtaacg taaagattta tgtgtccaat
     3421 cacctttatt tggaataccg attcgtggac tagcaataat atctttagga tatttacgat
     3481 tcttagtatc aatagacaat ctacagtcat ttaacgtagc gccatcgata gcccgtggaa
     3541 tgttaaatgc cttagtccat tttcctgggc cattcgttac ctcgtagcct ttcttacctc
     3601 tgttacggaa catagcggat aaaccttctt ctggttcaat tgcgcggata agtacgcctt
     3661 caggtatacc ttcagatctt gttacaaaat taatgagtaa atgcgtatgc atgacatgtg
     3721 cataaattgt accaccacgt ttatataatg acgtaacttt aggtgttatt ttaccgccat
     3781 agccatgagc cgcacgatca ttcaaaccta agtatgcttc cgtttccacg atgtagcctg
     3841 tatacgtttg agtggtatcc tgataaatca ctttgacacc taataagttt ttagcaattt
     3901 gtcttgtatc attattaacg aaatccataa tattctcctt gcaattatat gattaattta
     3961 aataaaaatg aaaaagatat ttttattgtg gtaaatgttt gataaaatag tattgttaag
     4021 aattataaaa atgtaaagta tatactttgt tatggaatat tttatttgca gagattgagt
     4081 ctcttttgaa cgcgaacatt gcagtgtgac atatttttag tatagttaac gaagcgttga
     4141 ctttcaaatg agtctccata tagatgcgta cttgtagcgg gttttcattt aactagtgat
     4201 gaaatataaa gttcattttc caaagtgaat gacgattgaa atgaagtggc ttgataatga
     4261 atcttgtaaa ttataacggt aataataatt aaaaatataa atatgattaa cgaaagggag
     4321 attacaatgg aacaattacc tcaagtaggt tttatgaaat cattcgtatt attttggaaa
     4381 aattatgtta attttaaggg gagatcaaga cgtagtgaat attggtatat gatgttgtgg
     4441 catttaatat ttatggttcc agctgtgtgt gtgctaataa taagcctatt tctattcttt
     4501 atttctatag cggcagataa cgaaacatta atagttatga gtatattgat gattatcctt
     4561 acatctgtgt atacttttgt ctatagtttg gcaacgttta ttccaaattt agcgttgtgc
     4621 gttcgaagat ttcacgatat atcaagaaca atgttatttc caatgattat gacagcattc
     4681 agtattatat tttatatagt attacagatt atcgatagtt attatgacag tgattttacc
     4741 ttaatgccga tgggtcttaa tattttgtta gtatttcttt attttgttta tttaagccta
     4801 acaattgtta tgatagtatt cctatgcttt gatagtaagc cggcaaataa atatggcgaa
     4861 agcccaaaat acccatcttc aattaaaaat caaccagtga catctgaaac accgaaaaca
     4921 ccgggagaaa cagataatca attgtaatga tataaatatc tcggcacaat gatgctggga
     4981 tattttttta ccaataaggc aataaaggca tggaaaaaag catatccaac actaatcata
     5041 cttaacatgc aaagatggta ctatttaaat ttttcgattt cagaaatttt aaaaataaat
     5101 tcactttaaa aatattgcat ttatagagaa tagaaatgtt atattaagat aaatacatat
     5161 tgttttagga ggcgaagtat gggacttgta attgaacatg ttacgaaacg ttttggcaag
     5221 atgacggcag taaatgatat ttcattagag ttagaatcag gcaaaatgtt aggttttcta
     5281 ggaagaaatg gtgcaggtaa aacaacgaca tttcgtatga ttttaggctt aagtgaacca
     5341 actgaaggac acattacgta taacggtaaa aaactagata aaacaatgta caatcgtatt
     5401 ggttatttac cggaagaacg cggtttacat gggaaattga cagttgaaga agaattgaaa
     5461 tatttggcaa ctttaaaggg aatgtcaaaa acagaaatac aacagcaaat atcgtattgg
     5521 cttgagcgtt ttgatattac tgaaaaccgc aaaaaacgaa ttgatagtct atcaaaaggg
     5581 aatcaacaaa aaattcagct gttagcaagt atgttacata aaccagaatt actaatatta
     5641 gatgagcctt tcagtggtct agacccagtt aatgttgagt tattaaaaga agctgttaaa
     5701 gacttaaatg attggggcag tacaatcgtt tatagttctc accgtatgga gcatgttgaa
     5761 gaattatgtg atgatgtttg tattttagat aaaggtcaac ttgttgtttc tggtgatatc
     5821 aatcatgtac gtgcatctaa tggtaacaag aaagttgtca ttgaatcaga aacaacacta
     5881 ccagatttaa caaacattag aggcatcatt catagtgaaa acatgaagca aggtcttcag
     5941 ttgacgattg agaatgaaga cgtagccaaa gatatttatc aagttgtggc acatcaaggt
     6001 tatgttaaac gattccaagt tgtagaacca tcattacaag atatattcat tgagaaggtt
     6061 ggtggcaagg atgcataagt tttgggcgac atttactttg acatatatca aaaaaattaa
     6121 agctaaatca tttgtaattt ttatgattat tattgctgcc ttaatgattg gcctatctaa
     6181 cattgataag attattaaca tgtttgatga tggtccagat aaaattggtg tagcagcacc
     6241 gaatgaacaa atctataagg ttttcaagca acaggcgaat acatttcata gtgatgctaa
     6301 gtttactaaa gtttctatag aagatgctga aaaagaagta aaaaaacata aacttgataa
     6361 agcttatatt atcaaggtga atcaaaatcg cacgttacaa gggacaatca ttagtgagaa
     6421 gcgagtaagt catgaagatt cacaaaaagt acaagcactg cttacagcta ttcaaacaaa
     6481 tatggtagcc ggagaattaa atatcaacaa agaagactta caaaaattac aagcacaaag
     6541 taaagttgat aataaggtga tttctaatga tgaagtcgat aaagtatcag aaggacaaaa
     6601 gattttcaac tatgctttag cttacggcat tatctttcta atgttcttta ttgttttaaa
     6661 ttatgcatct caaatagcta tggaaattgc tagtgagaaa acatcgcgtg tcattgaaat
     6721 gattattaca agtatttcac caatccaaca tatttttgct aaaattttag gcgttatagc
     6781 agttgccgtg actcagatta ttttaattgt attgatggca gttatatgca tttttgcctt
     6841 tgacttaaaa gacttgctac aaggttttaa cgtggaaatg aatcaattgt cttggcaaat
     6901 tattattgtc ggaattatta gtagtattgt tggaattatg gcgtatgtat tacttgcagc
     6961 cattctcgga tcattaacat ctagaataga agatttaaat caatctttga tgccactgac
     7021 attacttggt atgattgcat tttatatcgc gatattcacg ataaataatc ctgatgggca
     7081 acttgcttta atcacaagtt atattccgtt tttagcgcca tttcaattag ttgtacgcgc
     7141 tcaaacttca gggcttcaaa tacatgaggt catcttaagt tcattaatct caattgtaat
     7201 ggtagctgtc ttgatatgga ttgcaattaa aacatataaa gattcagtac ttacatttga
     7261 acgtggctta tttaattcaa tgaaacgtgt atttaaaaaa taaaattgat gagagtgaca
     7321 cacatttatc atataaaatg atgatgaaag cgtgttactc ttattttttt atatttagct
     7381 atagaataat gtctaaaaaa gtgtacaata tgaatgtgaa aacgaacttt ttaaaatggg
     7441 aatgtgaagt aagaagaaat gtaaacaaat gatatgtagc cgatagatta ttggtagcaa
     7501 atgtgtgatg gagtgatttc atgttatatc cgatttttat atttatatta gctggcttat
     7561 gtgaaatagg tgggggatac ctgatttggc tgtggcttag ggaaggacag tcttcacttg
     7621 ttgggctaat aggcggtgcg atactcatgt tatatggtgt cattgcgaca tttcaatcat
     7681 ttccatcatt cggaagagta tatgcagcat atggtggcgt atttatcatc atgagtttga
     7741 tttttgcgat ggtcgtagat aaacaaatgc cagataaata tgatgttatc ggtgcaatca
     7801 tttgtattgt aggtgtttta gtaatgttgc tacccagcag agcttgaaca cttgtggtta
     7861 gaagggagaa gtaaaaatga tagttgaagt tgagcaccaa agacaaggtg tagatttaat
     7921 caaaattgat aatgatgaaa ctaaaattat ttttacgaat tatggagcaa gagtagtgtc
     7981 ttggaaatat catgataata atattgttct tggaaatgtt gttgaagcgg atgaatttta
     8041 tcatagtaat ccttttaaat ttggtgcttc tataggaaga tatagcggta gaattgataa
     8101 tgctaaattt aagttgaaag gtaaagaata tcagttagaa aaaaacaacg gcgaacatca
     8161 tttacatggt ggatgtcatg gtttggacaa taagttattt gattatgaaa ttagaaatga
     8221 aattgctcaa attaaagtga tatttaagac ggtgttgaaa tcagctgatg atcatttccc
     8281 gggtgatatc gatgtgacga ttacacatat ttatgatgcg gatcaccaat ggtcgattga
     8341 atacgaagca gttgcatcag aagacacatt gtttagtcct acgaatcatg tgtacttcaa
     8401 tttgaatcga gataataatg tcgttgataa tcatcgcatt tcaagtaatc aattagatat
     8461 gtatgtatta gatgagcgaa atattgttac aggtgatatt ctagatttac atgaagtgtt
     8521 tgaagataac aagataaaat tgagtgatat ttttacatca caacatgcgc aattaagcca
     8581 acaaatgata cgttttggtg gtctcgatca cccatttaca gttggtgaac ataaaatgta
     8641 tgttgaaaat catgaattta tgttagaggt cgatacagat atgcctcatg ttgtattctt
     8701 tacttttaat cagcctgatg agtgggatag tccatttaat atttataaac cacattctgg
     8761 ctttacatta gaaacacaat ttttaccaaa tgatattaat ttatatggtg acaaagcacc
     8821 gtcaatttta aaagctggtg aagtgttcaa ttcgaaaaca agtttccgta tcgaagaaaa
     8881 agtagaaaat gattaaaagt atcggcattt atataatata tatgttgttt ggcggaattt
     8941 tgaataggtt atcaagaatc gaatcaaatg aagtgacatc attaatatac agtttgaaat
     9001 aaaaaaatat ataacataaa aaaggaatca atagcatcag tagttataga taagactatt
     9061 atacaaacga tgtgtaagtt gattcctttt gttttgaaaa gttcatttaa cacttataat
     9121 gaaaggtaat tatttcaatg ttctgttata cattttttga agtttatcgc gtcttgttgg
     9181 tgacagaaat ggattgttta gtgcatatcg taacagatct tgattttgtc tatcattata
     9241 ataaaggtca ttaagctgtt gtacagataa acgatgttcg attggtgctt cttgtattaa
     9301 gtaaagctga tcatttggag atacatagcc ttgttttaat acccggaaat agaaacctgt
     9361 tttaccagat gtagacatgc gcttcactaa atcaggaata ttatattttg cttgaatttt
     9421 ccagcatggt tctctaattt cagaaacctc aattaacgca tcacctaaac gatattgatt
     9481 accaaagtaa acatcagatt cgtctaaata atcaaatgtt aaattctctc caaacatcgc
     9541 atgagtaggt agtgagggta aatcatcttg atacagtgca tagttgtgtt tactaaaccc
     9601 acaaactgct ttatgtggcc caccatgatc tttataagcc tgttcatcac cagtgaaacc
     9661 agttgaagat aaccatgttg tttgcgaaat cttagtttta tctaaagcag agcgcatcgg
     9721 tcttttcgag ctataaggca aatcttggat ttttcctgtc gaaatcgcgt gaattgatat
     9781 catatcattc tccttaacga gtattattta aaatatattg tactataaaa catttttaga
     9841 attgtaaatt ctgattgata tgtttaaaaa aatgttagaa ataagggtat gttaatttat
     9901 aagagtcatg taaaatatga attgaataga ttacataaat atgggaggct accttcatga
     9961 aagatgtcaa agcactcaag ttaatgacat taaatgatgt tttaagtcaa atcaatggtg
    10021 acatgacgct gggaattggt acaggaagta caatggaatt actcttacct caaatggcgc
    10081 aactaattaa agaacgtggt tacaatatta caggtgtatg cacttctaat aaaattgcat
    10141 ttttagcaaa agaattaggt attaaaatat gtgaaatcaa tgatgttgat catatcgact
    10201 tagcaattga tggtgctgat gaagtagatc catctttaaa tataattaaa ggtggcggtg
    10261 gtgcgctgtt cagagagaaa gttatagatg aaatggcatc gcgttttgtt gtggttgttg
    10321 atgaaacgaa aatggttcaa tacttaggtg agatgtttaa gttaccagta gaggtggata
    10381 aatttaactg gtatcacata ttacgtaaaa ttgaatcata cgctgatata aaagtagaac
    10441 gccgtgtaaa cgaagatgtc gcatttataa ctgataatgg caattatatt ttagattgta
    10501 agttaccaaa aggaattgac ccatataagt tccatgagta tttaattcat ctgacaggtg
    10561 tgtttgagac aggatacttc ttagatatgg cagatcaagt tattgtaggc actcaagagg
    10621 gtgttaaaat tttagaaaaa taatatatag tatagaaaag cgcttgctag catcatcttt
    10681 ttaagaggta ctagcaagcg ctttgttatt atttgttttt atctgaagat tgttcttcag
    10741 ttttttcgat atgtttttct gcatcgatta caactgatga acgtctgtct tctttataag
    10801 tcgcaggttc ttttaatgat tgtttttcat cttcatttga atcgtttgtt aaatcgtcac
    10861 tttgtggtgc atgtttaact ggttctgctg attctactga ctccgaagtg atgtcatgat
    10921 cttcatcatg attagattca gtttgattat ctatgtgatg ttgagcttct gttttttcag
    10981 taccttcatg aatgcttggt tcgtcagcca ctgtattttc agtatcattt ttagcaatac
    11041 cgaccgttga tgcagtagct acacctgttt tttcagcatt tttaatattt gaagaagatt
    11101 tttcagcctc agtatgatta gtttctttat cgtcattgac atggtctaaa taattattag
    11161 gctcaagttc ttcaaggttt cgtcttgttg ttaaatatgc aatacctcgg ataattaagc
    11221 ttaaaccaat gtatcctact gcaacgattg ctgttgaaat ggcgatgact ttgattgaaa
    11281 atagatcgcc gatttcttcg ctaaacaaga aaataagtcc gaatgtcatt gaagcatgaa
    11341 atgtcgttgc aatataaatt gtacgtcctt tagtcgctct aattaattca ccaaggatca
    11401 tagagaatga gaatgtataa aggaagttat aagcagcaaa ttctgtacca taagttgtat
    11461 ttgctgagaa aactgaatac atcaaaccaa caacaatact tgcaaagaac gtgttcattt
    11521 tcgtttcaac aatattttgt aagtatgaac ggaatccgaa ttctactacg aaagccatta
    11581 gaatatgtcc aatcagaatg tgtgtaatag gtactgataa gcctgttgat tgtaataaaa
    11641 taaagctatc tgcaaatgta ttaaagctgt acataccaat aattagaata actaaaggta
    11701 aaattaaagc tagcaataga cgttcaatta ctttcaggct gattgagaat tttaaacctg
    11761 caagttggac ctttttatat ttgaaaacga gtatacaaat gattgcagca attaatggtg
    11821 ctagatctgt aacttcaaat ataaagtgtt tgacaccaat tatagactgg aaatctctga
    11881 gcataatgga taacgccatt gtaatgacaa agaagacgaa aatcgtcatt gcccattgaa
    11941 aaccagaaat tttattgttc ttcattatat gtaacctcca ttaggtaaca agcaaaatat
    12001 cttttagtaa caattataca tattacaagc cgagagataa attgtctatg tcaatattaa
    12061 tgaaaatgta atagtcttga cgtattttat taacataaat cagctaagta ttcgttatta
    12121 catagaaaat aggggtaaaa gtaatgtaga taagtatttt taagatacgg atttaactat
    12181 gaattgaatt caaaatttcc tatacgttat actttaattg ttaataagcc acataaataa
    12241 gagggggaaa tgctgtgtac aagcaaggtg aaccaaattt atggactgga aggttagata
    12301 gtgaaacaga cccgaaaaaa tttagacatt ttcaaacagt aacatttgaa gatttgtcta
    12361 agctggaaaa gagtagtatg ccatcagggg tcggtatatt aggctatgct gttgacaaag
    12421 gtgttgcttt aaacaagggg cgcattggtg caaaagaagg accagatgcg attaaacaag
    12481 catttgcagg tttgccggat ttgaatcaat gtgaaacttt agtcgattac ggaaatgttt
    12541 atcatgatca tgaggaatta attgatactc aaaaagaatt tgctatgctt gcagcgaagt
    12601 caattgctaa tcatagacaa acatttttat taggtggtgg acatgatatt gcgtatgctc
    12661 aatatttagc aacacgtaaa gtctatccaa cacaatctat tggtgtgata aatattgatg
    12721 cgcattttga tacacgtgct gaacaacaat ctacatctgg aacgagcttt agacaaattt
    12781 tggaagaaga tgaaaacaca gattatttag tgcttggtat tgctcaaggt ggtaatacgc
    12841 aaagtttatt tgattatgct aaagagaaaa agattgatta tgtctttgca gatgaattat
    12901 tgagtcacgt atcaccaaca attaaagata tgattgaacg ttttgtacat gaacatgatg
    12961 tcattatgtt tacgatttgt atggatgtca ttgatagtgc gtttgcacct ggggtaagtg
    13021 cgccggcagt gttaggtttg tatccacata ctgttcttga attggcaaaa cgtattattc
    13081 caagtgataa ggtgtcttca gttagtattg ctgagatgaa tccaacatat gacgctgaca
    13141 atagaactgc gaagctcgtt gctaatttag tgcatcattt tttaaaataa ttaaaaatat
    13201 ttatatgtga cgaaatttgc attgcagggc agaatgtgag tatttcgtaa tagaagttat
    13261 attaacgttg atatatttga aaaataattg cagtaagaag actcttaagt tgtagtcatc
    13321 ttactgctta tttatgttcg ttggtctgaa tgtaatgatt tatacatgga aatcataaaa
    13381 ttggttgtga agttgtttta cagttgacta cgcaactgca taagaggctc taataactta
    13441 agttaaagtc aaaaggtata cagttgacta cgcaactgca taagaggcac taataactta
    13501 agttaaaggc aaaaggtata cagttgactg agcaactgca taagaggcac taataactta
    13561 agttaaaggc aaaaggtata cagttgactg agcaactgca taagaggctc taataactta
    13621 agttaaagtc aaaaggtata cagttgactg agcaactgca taagaggctc taataactta
    13681 agttaaagtc aatggtgtac agttgactgc gcaactgcat aagaggcttt aataacttaa
    13741 gttaaagtca aaaggtatac agttgactgc gcaactgcat aagaggctct aataacttaa
    13801 gttaaagtca aaaggtatac agttgactga gcaactgcat aagaggctct aataacttaa
    13861 gttaaagtca aaaggtatac agttgactga gcaactgcat aagaggctct aataacttaa
    13921 gttaaagtca aaaggtatac agttgactga gcaactgcat aagaggcact aattacttaa
    13981 gttaaaggca aaaggtatac agttgactga gcaactgcat aagaggctct aattacttaa
    14041 gttaaagtca aaaggtatac agttgactga gcaactgcat aagaggctct aataacttaa
    14101 gttaaagagc ctctaaaagt ccatataaat aatcttggca ttgttgtacg aatgtatgta
    14161 cttgttcgga gtgtttacgc tttttatgat aattgatata aatttttcgg cctaagtttg
    14221 gtcgaatttt tttatattct aaattggacg tgtgaaatga ttggtaataa aatctcggaa
    14281 tgatagcgta acctaagccg agatgaacaa atccaactgc tgattcgaat cgatctgttt
    14341 ctacaacgac atttggacga atatttcttc tattaaaata gtcatctaag tgtttgcgca
    14401 cttgagaatt tttgtttggc aatatgagtg gcaaattttc aacatctacc caattttgat
    14461 ttttaaatgt ttctttaggt gctaataaaa tgtaagattc ctcatataaa ggaatggatc
    14521 ttatatcttc gtgggttatt ttttcatttg ttatagctaa atgaatatta aaattcagta
    14581 attgctctat agattgatgt ttgtcatgta tttcatataa acgatattgc tgttcagggt
    14641 agtcggaatg gtgctttcga attaaattcg caatccattg attcgtagat tcaagagtcc
    14701 caatttttat ccttggttct gatgtaacgc ttaaatcata cattttttcc atcgtggatc
    14761 gatattgttg aactaattcg ctcgcataac gataaaactg tattcctttt tcggtaatct
    14821 tgatgtcttt tgttgaacgt gtaaataagt cataacctaa atctgcttcc atttttttaa
    14881 tcgtagcagt taaagatggc tggctaatat gtaaaaattg tgcagcttta gtaaaactat
    14941 tatatttcac gatagccaag aaatattcta actgaataat tttcatgtgt tttgctcctt
    15001 ttatttatag attcaagcta tggataatta gtttatttat atttgttggc tatattttaa
    15061 catactataa tgaatattga agataataat gtaagcgttt taaatttagt ggaattaact
    15121 tctaaaaaga acaggagcga attttatgag aaaaattcaa gcaaaaaaag gtttaagtat
    15181 tgagtgtaaa ggttgggaac aagaagcagt acttagaatg ttatacaaca atttagaccc
    15241 agaagttgct gaaagaccag aagatttagt tgtttatggt ggtattggaa aagcagcacg
    15301 taattgggaa gcttttgaag caatagagaa aacgttacga gaattagaat cagatgagac
    15361 aatgttagta caatcaggaa agccagttgc tgtatttaaa acacatgaag aagcgccacg
    15421 tgtattaatt tcaaattcag tattagtgcc tgaatgggca aactgggatc attttaatga
    15481 attagataaa aaaggtttaa tcatgtatgg acagatgaca gccggtagtt ggatatatat
    15541 tggttctcaa ggtattgtgc aaggtactta tgagacattt gccgagctag gtaatcaaca
    15601 ctttaatgga gatttagcag gcactgttac actgacagca ggtttaggtg gtatgggtgg
    15661 tgcgcaaccg ttagcaatta ctatgaatca tggggtagca atttgcgtgg atgttgatga
    15721 aacacgtgtt gataagcgaa ttgatacgaa atactgtgat gttaaaacag ctgatttaga
    15781 tgaagcatta aaattagcag aagaggcgaa agaacgtgga gaaggattat caatcgggtt
    15841 agttggaaat gctgtagaca tccatcaagc gattctagaa aaaggattta aaattgacat
    15901 tattactgac caaacaagtg cacatgatcc gctaaatgga tatgtgccac aaggatactc
    15961 tgtagaagaa gcgaaagtat tgcgtgaaaa agatccgaaa aaatatgttg aactgtcaca
    16021 agcttcaatg gcaaagcatg ttgaattaat gcttgaattc caaaaacgtg gcgctgtagc
    16081 atttgattat ggtaacaata ttcgtcaagt agccttcaat aacggagtga aaaatgcttt
    16141 tgacttccca ggctttgtac cagcttacat tagaccatta ttctgtgaag gtaaagggcc
    16201 attccgcttt gctgcgttga gtggtgatcc aaaagatatc gagcgtgcgg atgaagaaat
    16261 gcgcaaactt ttcccagaaa atgaaaagtt attaagatgg cttgatttag ctgaagaaaa
    16321 aatttcatat caaggactac catcacgtat tgcttggtta ggctatggag aacgagcgaa
    16381 aatgggctta gctttaaatc gtcttgtacg tgacggtgaa atttcagcac caattgttat
    16441 tggacgagat catttagatg ctggttcagt tgctagtcct aaccgtgaaa cagaaagtat
    16501 gaaagatggt agtgacgcgg ttggtgattg ggctgtattg aatgcactca ttaacacagc
    16561 tgcaggtggt tcatggattt cattccatca cggtggcggt gttggcatgg gatattcact
    16621 tcatgcgggt atggttgttg tagcagatgg atcagagcgt gctgaaagaa gattggaacg
    16681 cgtattgacg actgatccag gtatgggtgt tgcccgacat gttgatgctg gctatgacat
    16741 cgctattcaa acagctaaag aaaaaggtat tcatattcca atgattgata aagcaggtga
    16801 taagtaatga atgatttaat aattaatcat atagcagaat taattttacc gaaatcaaca
    16861 gataaacctt tgaagggtaa ggaattagat gaattaaatg ttgtgaaaaa tggtacagtt
    16921 gtcattaaag atggcaaaat tgtatatgct ggaacacata cagatgacta cgatgcgact
    16981 gagacgattg atgctagtgg gaaggtagtg tccccagcat tagtagatgc acatacacat
    17041 ttaacatttg gtggatctcg agaacatgag atgtcattaa aacgtcaagg taagtcttac
    17101 cttgaaatat tagaaatggg tggtggcata ttatctaccg ttaatgctac tagagaaaca
    17161 tccgaagatg acttgtttaa aaaagcagag catgatttgc ttactatgat taaacacggc
    17221 gtgcttgcag ttgaaagtaa gagtggttat ggattagata gagaaaatga actgaagcaa
    17281 ttgaaagtgt ctaatcgttt agctgagaaa tatgatttag acatgaaaca tactttccta
    17341 gggcctcatg ctgtacctaa agaggcaagt tcaaatgagg catttttaga agaaatgatt
    17401 gcgttacttc cggaagtaaa acaatatgca gactttgcgg atattttctg tgaaacaggt
    17461 gtatttacaa tagaacaatc gcaacattat atgcaaaaag ccaaagaagc aggttttaaa
    17521 gtgaaaatac atgcggatga aattgatccg ttaggcggac tggaattagc aattgatgaa
    17581 caagcaatat cagcagacca cttagtagct tctagtgata aaggaaaaga aaagttgcga
    17641 aatagtgata ctgtagctgt cctcttacct gcaacgacgt tctatttagg taaagaagat
    17701 tatgcagatg caagaggcat gcttgacaat aacggtgcga ttgcattagc aactgattat
    17761 aaccctggta gtagtgtcac aaacaactta caacttgtta tggcgattgc agccttgaaa
    17821 ttaaagctat cacctaatga agtttggaat gctgttacgg tcaatgctgc taaagcaata
    17881 gacattaacg cgggtacaat taacacaggt gataaggcta atttagtaat ttgggatgca
    17941 ccaaatcatg aatatattcc atatcatttt ggtatcaatc acgcagaaaa agttattaaa
    18001 aatggtaagg tcatcgttga taacacatta tcttttaaag cataaagtaa ttaaagctct
    18061 aagcgttggt ttacactaat gtttagagct ttttgtgatt tgtaaaaaca agttaattcg
    18121 agagcacgtt tttgtatagg ttcttaatta taaaagttag aatagtttga caatagtgtt
    18181 acgatattat taaacaattg tctatcatta gcgaaagtaa ttaacaagag tacattgttg
    18241 tgaaatcatc gtttaatagt taaagatgat catcgtaggg tagacaacat atcattgact
    18301 taaggaaggg gaatgaccga tgggattaaa agatcaagct gttgcctgga gaaggtattt
    18361 tcatcaattt ccagaactat ccgataaaga atttaagaca acgcaaaaaa ttaaagatat
    18421 tttaacagaa catcatatta gaatattaga cttaccgctt gcgactgggc ttgttgctga
    18481 agtgggacaa ggaccgagtt gtatagctgt tcgggctgac attgatgctt taccgattca
    18541 agagcttgtt gaacaggatt ttaaatctaa aaatgaaggt gtgatgcatg cgtgtggtca
    18601 tgatattcac atggctagta ttttggctac agctgtaaag ttaaaagaaa ttgagggcac
    18661 tcttactggg cgtgttaaat tcatttttca accagcagaa gaactaggac atggcgcttt
    18721 taaaattatt gaaacacatg cattaaaaga tgtacaagca gtattgggtt ttcataatga
    18781 cccatcgcgt tcggtaggta catttgcaat caaaacaggg gcaattacat cagcagtaga
    18841 tcgttttgag tttcatatta aaggcgtggg tggtcatgct gcaaagccag aacaatgcaa
    18901 cgatccagtt attgtgttgg cgcaattgat taatagtatt caatccatag ttagtagaaa
    18961 tctatctgcg tttgatgaag cggtagtaac cattgggcaa atatcatgtg gtaatacttg
    19021 gaatgtcatt gctgaccatg catatgtaca aggcacagta cgtagttttg atcctgtggt
    19081 gcgaaagctt gtagaaacac gattacaaaa tattgcggac gggttagctc aagcatataa
    19141 tatgaaaatt aatttgaatt atacacattt acctggtgct gtgatgaatg atgaggtatt
    19201 gacgcataaa gcaatagcag tagcccaaca tgttggatat aagatggaaa tgatggaaca
    19261 gccgcttacg attggagaag atttttcagg ttatagtcaa cattttccta gtgtatttgc
    19321 gttaattggt tctcatagtg aatatgattt acatcatcct caatataaac cggatgaaag
    19381 gattctggaa aaagtacctg agtattttgt tgaatttgta aagagactat tgcatgagta
    19441 attcgagctt gttttttaaa gaagaaagta atttttagcg taatcatgat taagttcctg
    19501 ggaagtttgg gtaaataata accattaagc gaataaggag atgttaaaaa tggcagctca
    19561 agatcctaga acaaaattta aaacaacaga ttatgaaaaa caagaacaag aggtaccggg
    19621 tttacaatct gaaatgacgc cagcacctga ttgtggcgaa acgtcttacc aaggtcatca
    19681 acgcttagaa ggctataaaa tgttagttac gggtggtgac tcagcaatag ggcgtgcagc
    19741 agcgattgcg tatgctaaag aaggtgcaga tgtagcgatt aactatcttc cgagtgaaga
    19801 acaagatgca caagaagtac gccaagtgat tgaagaaagt ggtcaaaagg cagtgttaat
    19861 tcctggtgat ataagagatg agcagttcaa ctatgacctt gttgaacatg catatcaaca
    19921 attaggtggc ttagataatg ttactttagt tgctggacat caacagtatc atgatgatat
    19981 tcatggattt acaactgagg catttacaga aacatttgaa acgaatgtct atccgctatt
    20041 ttggacagtt caaaaagcat tggaatattt aaaaccgggt gcgtcaatta caacgacatc
    20101 atctgtacaa ggctataatc ctagtcctat tcttcatgat tatgccgctt caaaagcagc
    20161 aattatttca ttaactaaaa gtttttcaga agaattagga ccaaaaggaa ttagagtgaa
    20221 ctgtgtagcg cctggtccat tctggtcacc attacaaatt tctggtggac aacctcaaag
    20281 taaaatccca acatttggtc aaaaaacacc tttaggtcgt gcaggtcaac ctgttgagtt
    20341 atgtggaact tatgtgctat tagcgtcaga agaatcaagt tatacaactg gacaagtgtt
    20401 cggtgttagc ggtggagtgc aaatagatta ggtttgtatg aagcgtttaa ttgaacaaaa
    20461 taatattaat ttagctactt caatcaagta aattaaaatg aattagtgaa gtgtatttta
    20521 aatagtcggt aaaggtgtgt atctttatcg gcttatttta ataccctgtc ataaagagcg
    20581 tttgctcagg gtgtggatta taagttttaa gcagaataaa ctttcttaat gaggatgtca
    20641 ggacaacaac gaaaaagtta aaaactgttg cgggattggg tttagggacg gttagtggaa
    20701 gaaagttaaa tttattaaca attgaaatgc ctaaggttag atattaaacg tcagaacgga
    20761 caaagaataa taggatatgt tttcaattat atagcgaagt atttatatgt taagatgatg
    20821 tgaattacat agaaacgctg cccgtagaag tactgatcat agaaatactg ataaacgtaa
    20881 atttgcaaac tcgatattaa acaaaggggt tgttattatg gcattaatag tatctagagc
    20941 tatattattt ttgattttag tattagcttt tggaatagcg actgtttttc ctgaacaaaa
    21001 gttgccagtt tatatattat tcacactgtt tgtagtggtt gcgattgttt cgaatgtttg
    21061 gcttacacat caaaacgtta aaaagcatat cattaagttt gacaaacgtg ttgcttactt
    21121 tttgggtgtg ctctcaggag tattctacat tggaatcgtt atttatggat caactattat
    21181 aggttactat aattttatat catttattat atttgcaata agtatcgtat tggttcaaat
    21241 attagaaaat atactgggta ttaattttaa aaatacgctt ccatcaaaca aacagttgaa
    21301 agtcatctcc aagcaacgtg ttcagcaatt taaagtcatt tcattaatat tagtaagtgt
    21361 gtctatcgtg ttcattttta ttgcattaat gcttaaatgg tggcttttac ttgttattag
    21421 cattattgtt ttgattatta ttacagtagc aatgattgtt tatgaaaaat attatacgac
    21481 tcctgattaa tttatgattg acgtactaag gattatcttg taacgtaaac gtaaataagc
    21541 atcacttatc tagagaggtg gagggactgg ccctatgaag cctcggcaac atctcgaatg
    21601 tgccaattcc agtaaccgta atggtttgaa gataagcagg taaagcacat gaaaacctct
    21661 ttcttcatcg tttgtgagaa agaggtattt ttaattggaa agcaggtaaa aaggatggaa
    21721 gtacataaaa agagcaatgc ttgggcatta ttccccttgt tattatttgt ggcgttgttt
    21781 ttaggcgtag gtattatcac aggtgatttt acttcaatgc cattaaatgt tgcaattacg
    21841 ataacggtaa ttgtggcgtt attaatgaat cgaaaagaat catttgctaa aaaagttgag
    21901 gtcttcacga aaggtgcagg ccattcaaac attattttaa tgatgttgat ttttatttta
    21961 gcaggtgcat tttcaaatac agctgaaaaa atgggtggcg taaagtcgac agttaattta
    22021 ggactatcat taatcccgga aaatttaatt attgtaggat tgtttgtgat ttgtatgttt
    22081 gtttctatat ctatgggaac gtcagtaggg actgttgctg caatagcacc tgttggttac
    22141 ggatttgcac aagcgacaga tgtaccaact gcgctagcaa tggctacagt tgttggaggt
    22201 gcaatgttcg gcgataatct atcaatgatt tcagatacaa caattgctgc ggttagaaca
    22261 caacatacga aaatgaaaga taaattcaga gtaaacttta aaattgtatt gccaggtgct
    22321 attttaacga ttattattct gtatttctta acgaatggca tttctttaaa tcatgctaaa
    22381 aactatgatt ataatctaat taaagttgta ccgtatattt tagtattggt attagcatta
    22441 ttaggtgtaa atgtcattat cgtattgatt ggcggaacat tattagccgg aatcattggt
    22501 ctcatagatg gttcattcgg ttggatgggg ctactagatg cagtgtccaa aggtattata
    22561 agcatggaag atattgctat gattgcgttg ctaattggtg gtttagtggg tattattcaa
    22621 cacaatggtg gtattgaatg gttgttgcaa tttgttcgct ctaaagtgaa atcaaagcgt
    22681 ggcgcagaat taggaattgc tagtttggta agtgtcgcag atattgccac ggcaaataac
    22741 accatctcta ttattatgtc aggtccttta gctaaaaata ttgcagatga atatgatgta
    22801 gattcaagga aatcagcaag tattcttgat atatttggag gctgcttcca aggtttatta
    22861 ccatatagcc cgcaagtcat ttcagcggct ggtgtagctg gtatttcacc gttaatgtta
    22921 ttcccataca gcatttatcc tatattatta ggtgtatgcg gacttgttgc gattttattt
    22981 aattttccaa agctaaaaaa gaacactagt caagaaatga aacattaatg cttaacaata
    23041 tatcgatgta gccaagtaag gtggcttgtt atattaacag atcatgttgt actcaccgta
    23101 taacgctaat cttttaaaaa tgatgatcat ctatgtttgg aactgagcaa aaatacgtta
    23161 aaataaaagt aagagcgaaa gtgggtggac aacagtggca aaatttaatg tagagaatga
    23221 acatgtcgaa gttgaaattg aaaagcttta taaattttct ccagagttag tatacgaagc
    23281 ttggacaaag aaagatttgt tgaaacaatg gtttatgaca tcagccagaa cgaataaaga
    23341 gattgaagcg gacgttaaag aaggcggtaa atatcgtatt gtagatcaac aacgtaatgg
    23401 caaagtaaat gtgattgaag gcatttatga atctttagtt atggatgaat acgttaagat
    23461 gactatcggt atgcctggac taagtgaaac acaagatgtc attgaggtag aattttttga
    23521 acgtgaaaca ggtggcaccc aaatgctatt ttattatcgt tcattagttg aaaaagaaag
    23581 acgatttact aatttagaat acaagcaaaa gaaaaaagaa tatcatgatg ccatggtgca
    23641 tggtttcgaa ttgatgtttg ataagatgta ccacgttatc gaaacatcaa cacaacaata
    23701 aaatgataaa aggaagttat gataggctca aggccattaa atcataactt ccttttttct
    23761 aatttgaaat atcattatgt agtaaatact cattatattt tcttacaagt gtttcaaaga
    23821 atatatgata actgatgaag ctagaatagt ctgtaccatc agttaagtag aatgtgttac
    23881 tggatacata gacgttgtaa tttgcttttt gagcaagggt attatctttc attgtagtaa
    23941 cagaaataaa gtatttttgt ctaagttgaa gcaagtttgt tacttctaat aattcatgtg
    24001 tttcacctga taatgaaata ataaagaata aatcattagg ttcactctta ttcaaaatca
    24061 ttttaagttc atgttcatca tgaagtacaa tgacatgatt atgtaacgtt aacaatatac
    24121 gctgtgcctc tgaagcaacg tttaactgtg cacgccctgt accatacaaa tatattgttg
    24181 aagctgtatg cattttttca gtaagaagct ggtaatcaac gcgttctaaa taactgaatg
    24241 tactatcgat ttcttgtttg aaactatcga tagagttaga tggtaattgg tgtgtcttat
    24301 tactttcaaa tttaaggtac gatttaaaat cgctataacc gtcaaaacct aatttgcgtg
    24361 taaatctgtg aatggttgca ttcgaggcat gtgtgaattg cgctaaatct tgtattttca
    24421 tatttttaca ttcatctata tgtgtattga taaagtgagc gatatgtaaa tcattgtcat
    24481 taagtttatc aaagtttcga ttaatgtgtt cgtctaaaaa catattcaac ctccttggaa
    24541 ataaatttgt gatatatggt ttttacatta aaaggtgaaa tttttaaaat ttaattcttg
    24601 attgtatata aggttataaa tggctaaagt acttacatat tttaaaatca agcatattct
    24661 tgacatgctt gtgaaaaaat tttcactata tgaaaagata atctgattat acaaaatact
    24721 ttcaaaatac gtcaatgtcg ttgtaggtgt gtaagcgttt actatatatt atatttaaat
    24781 agtaagacat ctatttataa atgaatctaa ttagatgtat tgttaggaaa gatgatatgg
    24841 agggaataga agatgaatgc gataaagcgt ttcggtagtg caatgattgt accagtatta
    24901 atgttcgctt tctttgggat agtgcttggc tttgcgacat tatttaaaaa tcctacaatc
    24961 atggggagtt tagcagatca acatacgttt tggtttaaat tttggtcagt gatagaatct
    25021 ggtggctggg ttatttttac gcatatggaa gttgtatttg ttgtaggttt accactttct
    25081 ttagctaaaa aagcaccagg tcatgcagca cttgcagcat tgatgggtta cttaatgttt
    25141 aatacattta taaatgctat tttaacgcaa tggccacata cgtttggcgc aaatttagaa
    25201 aaaggcgttg aaaatgtacc tggtttaaaa tcaattgcag gtattgccac attggatacg
    25261 aatattctag gtggtattat tatttcagct attattactt ggattcacaa tagatactat
    25321 agtaaacgat taccagaaat ggttggtgta ttccaaggtt taacatttgt ggttacaatt
    25381 tcattttttg tgatgttacc attagcagca attacttgtg ttatttggcc aactgttcag
    25441 cacggtattg gttcaatgca acactttatc attgcttcag gctatatcgg tgtatggttg
    25501 tatcacttct tagaacgtgt attaatacca acaggattac atcactttat ttatgcacca
    25561 attgaagttg gaccagtagt agtcaatcac ggcttgaaag ctgaatggtt acaacattta
    25621 aatgagtttg ctaagagtac taagccatta aaagagcaat tcccttatgg ctttatgctt
    25681 caaggaaatg ggaaagtatt tggatgttta ggtattgctt tagcaatgta tgctacgaca
    25741 cctaaagaaa atcgtaaaaa agtagcagca ttacttattc ctgcaacatt aacagcagtc
    25801 gttgtaggta ttactgagcc attagaattt acattcttat tcatcgcacc atacttattt
    25861 gttttacacg cagttttagc agcatcaatg gatactttaa tgtacgcatt tggtgttgta
    25921 ggtaacatgg gtggtggctt gttagacttt atttcaacaa actggttgcc attaggtaaa
    25981 gaacattggg gtacttatgt tgcacaagtt attatcggtc tgatatttgt agcgatttac
    26041 ttcttcttat tcagattctt aattttgaaa tttgatattc cattacctgg acgtaagaaa
    26101 acagaagagg aagttaaatt attttctaag caagattaca aaaataaaaa aggtgatagc
    26161 gtagattcta aacgtgcttc aacaggtaat gaatacgaag acaaagctgc gtattacctc
    26221 gacggtttag gcggcaaaga aaatatcaaa gatgttacta actgtacaac aagattacgc
    26281 ttaactgttt atgatgaaag taaagttgcg gatacggaat atttcacgca tcaacaaatg
    26341 gcacacggat tagtaaagag tggtaagagt atccaagttg tagtaggtat gacagtacca
    26401 caagttcgtg aagcatttga acaaatggtt gaggatcaat cgtccgaaga taaataatat
    26461 aaaaaaagac acatcactat acgcaatcat tgtaaatgat catcgtatag atgtgtcttt
    26521 tgttatgata tacgagcttt gctcatagat gacgatctat aattggcaga tagaatgtcg
    26581 atatttctag ttgcaatcgt atgtaacaat ttagcaaata tttctggcgt acatataata
    26641 tgtgcatgag acttattcaa cgtataagtg atatcagtgt gattttggaa catattcata
    26701 atatcgatga ctttgtcttt aatgaaagca tcttgcaagc catcaatatt gatatcgaaa
    26761 cgctttgcag attgatataa attatcagaa tcaagtaatt catttatttt ctttggtgaa
    26821 agtacttgga aaatactata ctgatgaaaa cttaaaaatg aaatgaagta tggaagttgt
    26881 tgctttggta agctaatttc taatacatac tgattaaact gtttgtttat tgagaattgc
    26941 aacgcatcac aaaattgtac acttttgcat aacttactta atttttttct agtagtacgg
    27001 tcatttaaac cggcgatgtt gatgatgtat gtttcgaatt tatcaattaa catgtgagga
    27061 cctcccgagg aatacatggc attaaataca cgtttaatat ttataaaggt gacttaattt
    27121 tgttcaagtt gattttacca cgcttttttt ctttattcac taagactttt gaatgaagtt
    27181 taaaataatt gtttatcagt gataaaatat ttgcaataag aagagaatgg ctaaataatc
    27241 ttaattttca gaaaagtaat tgtaacctta ctggtcttat ggtaatattt ttcaatatta
    27301 tcgacgagga tgtgttaaca atgtttcaaa aattgagtat atttgccacg aagagttttt
    27361 tggtatggat gttagtagca gctgttattg gatttatttt cccacaacat gttgcgacat
    27421 taggtaaatg ggtaccttat ttacttggta ttgttatgtt aggtatggga ttaacaatta
    27481 cacctaatga tttcaaaatg gtctttaaag cacctagagc agtaattatt ggtgtctgtc
    27541 tacaattcag tattatgccc acattagcat ttataattgc aaagtctttt catttaccac
    27601 ctgatattgc tgttggcgta atattagttg gatgttgtcc gggtgggaca tcaagtaatg
    27661 taatgagtta tttagccaaa gctaacgtag cactttctgt ttctattacg acggtctcta
    27721 cgttgctagc gccattcgtt acacctgcgt taatatatct atttgcaaat gaatggttgg
    27781 aagtatcttt cctgagtatg ttgtggtcag ttgttcaagt tgtattaatt ccaattgctt
    27841 taggtattgt tttgcaaatt attaatcgta aaattgctga aaaagcttct acagctttgc
    27901 cgattatatc agttgttgct atttcattaa ttttagcaat agttgtaggt ggcagtaagc
    27961 accaaatctt aactacagga ttattaatat ttttagtagt tattttacat aacgtattag
    28021 ggtatacgat tggatattgg ttagctcgtc ttttaaaatt agatcgacaa gatcaaaaag
    28081 cagtcagtat tgaagttgga atgcagaact ctggtttagc tgtgtcatta gcagcattgc
    28141 attttaatcc aattgcagca gtaccaggcg cagtgtttag tttcattcat aatataacag
    28201 ggcctatttt agcaaagtat tggtcaaaaa agttataatt gcactaatag aatgaagtgg
    28261 tcatcggact atgttaagct ttgataaaga gaaaaaatag aggagtaaat atatgtatag
    28321 agcagttata tttgatttcg atggaacaat aatagatacg gaacaacatt tatttaatgt
    28381 tattaataaa catttagaga tgcataatgc cgatcctata agcattgatt tttatcgttc
    28441 ttctattgga ggagcagcta cagatttgca tgaccattta attaaagcga ttggttcgga
    28501 aaataaagat aaactttatg aagaacatca tcttactagt acaacattac cgatgattga
    28561 tacgattaaa tcattgatgg catttttaaa gcaacgtcac attcctatgg cgattgccac
    28621 aagtagtgtg aaagcggaaa taatgcctac ctttaaagca ttaggtctag acgattatat
    28681 agaggtagtt gttggtagag aagatgttga acaagttaaa cctgaccctg aattatattt
    28741 atctgcagta caacaattaa attatatgcc gacacaatgt ttggctattg aagattctgt
    28801 aaatggtgca acagccgcga ttgcagctgg attagatgtt attgttaata cgaataaaat
    28861 gacaagcgca caggactttt ctaatgtaga ttatgtagca aaagatattg attacgatca
    28921 aattgtagcg cgtttcttta cgaaatagga ggcgtatcat gatgggttac attatattgt
    28981 tttttctagc tggtccagta attttaggcg ttggaaattt ggtgattggt cctatattta
    29041 acaaacagac accatttcac gtgcaagtaa gatcttttgt tgttggttca atgatttact
    29101 taatactcgc aacaattgac tattttttac tattacaagg taaactttaa cgagaaaacc
    29161 accttacctc attaaatgga cgaccatatg tatgtgaaat ggtagaacgt tcatgtttat
    29221 gtatgagata gggtggttta aatagttaca tatattttaa taataacgtc acgatgataa
    29281 gtacaattaa gataatatct atgcctacca taattgtagc tcttgttgca ttacttcctt
    29341 gttcttttgc tgatttcata gcacggtagt ttggcacaaa gctaataatt agtaagatta
    29401 atacaattac accaattaat gctgttgtca tgatgaacga cctcctttat ttttttcaat
    29461 caattcccaa ataaacgtag caatcacacc gatgattaac ccagtgatga caccgatttg
    29521 taccgttaaa aatatggcgc cgaaaatgat accaataatt aatgcaatcg gtaaagtcgt
    29581 accgagttta atcttgcgct ctggagaatt aataatagta aataatgtaa gacaaatgag
    29641 tatgaaaaca agtgttgcaa taatagttct tccaactaaa tataggatgt caggtttttc
    29701 cataccgata taatttatga tgaaaaatgc tacagcaaag agtatcgata tttttgtagc
    29761 acgtggcagt atttgtttta acattgatat actcctttgt aatattatta aaattatatc
    29821 ataattaccg agaatagctg aagttgtatg tgacttaata gttttcgaac aactttttta
    29881 tttttttaga aaaatcacaa aataattgtt tgcaaagttg caaaagcctg ctatagtagt
    29941 tctgtaaacg attgcatggt atgcaaatat taatgtacca aaatcggtaa tttatagtat
    30001 aattacggca ataagttttt ttatggattt atttagtatc aatcagagat ggggtaagaa
    30061 gttatggaga acaatgaact acaaagggga ttgagtgccc gtcaaattca aatgattgca
    30121 cttggtggta cgattggcgt ggggcttttc atgggtgcta caagtacaat taaatggaca
    30181 ggcccatcag ttatccttgc atatttaatt gcgggtatct ttttattttt aatcatgaga
    30241 gcaatggggg aaatgattta tttaaaccct acaacaggat catttgcaac atttgcaagt
    30301 gattatatac atcctgcagc aggttatatg acagcatgga gtaatatatt ccaatggatt
    30361 gttgttggta tgagtgaggt catcgcagta ggagaatata tgaagttttg gttcccggaa
    30421 ttgccaacct ggattcctgg tattattgct attttattat tgatggcagc gaatttattc
    30481 tcggtaaaag cgtttggaga attcgaattt tggtttgctt taattaaagt tgtaacaatt
    30541 attttaatga ttattgctgg ttttggtctt attttctttg gttttggaaa tggtggccat
    30601 gcggtaggta tttctaatct atggacaaat ggcggattta tgccaaatgg tattgttggt
    30661 ttcttctttg cattatcaat tgtaattggt tcataccaag gtgtggaact gataggtatt
    30721 actgcaggtg aaactaaaga tcctcagaaa aatatcgtta aagcagtgaa tggtgttatc
    30781 tggagaattt taattttcta tttaggtgct atttttgtta ttgtttcagt gtatccttgg
    30841 aatcaattag gagacattgg aagtccgttt gttgcaacat ttgcaaaaat cggtattaca
    30901 tttgcagctg gattaattaa ctttgttgta ttaactgcag caatgtcagg atgtaactca
    30961 ggtattttca gtgcgagtcg tatgatttat acacttgctc ataaaggtga aatgccgaaa
    31021 atatttacta aaatcatgag aaatggtgtg ccattgtaca ctgttgttgc agtatctctt
    31081 ggtattttaa ttggtgcttt attaaacgta attttacctt tatatatcga tggagcgaag
    31141 agtattttcg tatacgttta tagtgcgtct attttaccag gtatgattcc ttggtttatg
    31201 attttattca gtcacttacg ttttagaaaa ttacaccctg aagaagtgga aggtcaccca
    31261 ttcaaaatgc ctggtggcgc agtaactaac tatttaacaa tcttattttt aatattagta
    31321 ttggttggta tggtatttaa tgttgaaact agaatatcag tgcttattgg tgttatcttc
    31381 ttaacgattg taacgattta ttattttatt agatataata aaaataatgt aaaagcaaaa
    31441 tagtatgatt ataatgtgcg cttattttat gacaaacttg gccctagctt attaaagtta
    31501 aatagccaaa gaaatgttat gagtaggcgc atttttttat aaatatagct taaatcaaag
    31561 atgtagtatg aatttactta taagtggcaa tactatcgtt agtttttact gtaggcttta
    31621 aaataaaaaa tagctttatt aaaataacaa acgcaattga tatatggaat gaatccgtta
    31681 atcaactgcg tttgttttta atataagaaa ttgaatggcc ttatcaaatc attggtcata
    31741 tgcgtatgtt atacaagata aaagacatgt aagctttgaa aatgatgcat aaattattat
    31801 atgacatgat taaagaattt atataagact gttaatgttt aaagttaata tttgataaat
    31861 gtttacgata gttatctaaa gccattttag attgtgttat gaaatctaat gatgcgtgat
    31921 aatttaatgc aacataacga taatataaaa tatcaatagt gaacatttga gcaaataatg
    31981 aagttgttgc tcccatatgc atctcatttt catcagtttt cccataagtt aaaacaatat
    32041 ttgatgcctg tgctacggga ttatcccttg tactagtaat tgtaattata ggtatatggt
    32101 agtcatcaat aactttaacc attgattgca tttcactttg cgtaccattg ttagtaataa
    32161 gaataacact gtcattcgaa ttgtgagttg ctaataatgt tgcaaaaata tgtgtttctt
    32221 gaacaagttg aatattaaga cctattcttg ataacttttg gtataagtcg gtagcaacta
    32281 caaaagatgc gccaaaacca tatataaaaa tcgtctcaga acgttttaaa caatgacata
    32341 tttgatcaat agttttatca tttaattcgt tatttgcatg attaagtgcg cgtgtagtac
    32401 gtgtatggag tttagttctt aaagattctg tgctttcgtt attcattaat tcaacattgt
    32461 aaattgatga tgctttagga acatatttag atatatttat tttcaagtcg tgaaaaccgc
    32521 catcagtaat tttcttgcta aatcgaatga ctgatgatgt actaatatct aataagcttg
    32581 ctaagtcttg agacctcatt ttaatcacct tttgaggtga atttagtata aatttagcta
    32641 tctttttttc atttttagtc atatatggat attgactatc tatttctgtt agtacgtttg
    32701 acatattaat cactccactt taacgcaata tattttatta gcgttggcta ttgttgttta
    32761 acgcgatggt atttacaact tgtaacgtta gagaatgaaa aataagctga atatgttgtt
    32821 tgtttaatga tattgaatta taaaactgta aatt
//
LOCUS       DAHHOJ010000019        32690 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.019, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000019 DAHHOJ010000000
VERSION     DAHHOJ010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32690)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32690)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32690
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.019"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(107..1339)
                     /locus_tag="NBH32_000754"
     CDS             complement(107..1339)
                     /locus_tag="NBH32_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HCD1514381.1"
                     /translation="MGRNLKLKHRESDFEFTKNHKRLLLGSVFLMATSAIGPAFLTQT
                     AVFTSQFLASFAFAILLSIIIDIGAQINIWRILVVTGLRGQEISNKVVPGLGTVISIL
                     IAFGGLAFNIGNIAGAGLGLNAIFGLDVKWGAAITAIFAILIFVSKSGQKIMDVVSMI
                     LGIVMILVVAYVMFVSNPPYGDAFVHTFAPEHPMKLVLPIITLVGGTVGGYITFAGAH
                     RILDSGIKGKQYLPFVNQSAIAGILTTGIMRTLLFLAVLGVVVTGVTLSSENPPASVF
                     EHAIGPIGKNIFGIVLFAAAMSSVIGSAYTSATFLKTLHKSLDERSNLIVIVFIVIST
                     MIFLFIGKPISLLIIAGAINGWILPITLGAILIASKKKSIVGDYKHPNWMFIFGIVAV
                     LVTILTGIFSFKEVLQLF"
     gene            complement(1351..2103)
                     /gene="pxpA"
                     /locus_tag="NBH32_000755"
     CDS             complement(1351..2103)
                     /gene="pxpA"
                     /locus_tag="NBH32_000755"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCD1514382.1"
                     /translation="MRVDLNCDLGEAFGNYSFGGDHQIIPLITSANVACGFHAGDENV
                     MNETVKLAKAHNVAVGAHPGLPDLKGFGRRNIDISNDEIYNLMIYQLGALQGFCRIHQ
                     VKINHVKPHGALYQMGAKDREIASVIAQAVYDFDPSLVLVGLANSYLISEAKNVGLIT
                     ASEVFADRRYEDDGQLVSRKESDAVITDTDEALKQVLKMVKENKVISKNNKEVTLQAD
                     TICVHGDGEHALLFVSKIREILMKEGIDIQSL"
     gene            complement(2103..3464)
                     /locus_tag="NBH32_000756"
     CDS             complement(2103..3464)
                     /locus_tag="NBH32_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1514383.1"
                     /translation="MLRCLIANRGEIAVRIIRACREYGIETVAVYAKRDEQSLHVHLA
                     DQAICIGEANALDSYLNIDRIISAAQITGANAIHPGYGFLSESTKFAQTVEEQGIAFI
                     GPTKKTMEMMGDKITARQTVHHAGVPVIPGSNGAVNHVSEIEKLAKDIGYPVVIKAAS
                     GGGGKGIRIVKKAEDLEKAFKEAKSEGKKYFDDDRVYVEAFIPVAKHVEVQVMGDGQD
                     NYVHLGERDCSVQRKNQKLIEESPCAALTEERRQQICNDAVKVARAANYRSAGTIEFL
                     VTDTAHYFIEMNARIQVEHTVTEMRAERDLVAAQLYLLEHNHLPFSQSDIQFNGHVIE
                     ARINAENPEKKFQPTPGKVTALHLPQGFNVRVDSLLYHGYQVSPYYDSLVAKVIVKSH
                     DRASAIDKLKATLDEMVIDGFSTTADFLYAVLNYPLYRDGDAKDVDIKFLEKHQIVKG
                     VEL"
     gene            complement(3478..3927)
                     /locus_tag="NBH32_000757"
     CDS             complement(3478..3927)
                     /locus_tag="NBH32_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HCD1514384.1"
                     /translation="MNIEKIEQIIKLVKENDVKKFKYKNFEDEIEINFTDSNHLAAHS
                     NQSNQSMNNNDLTASKANDNSDVSTNDYHDIKSPMVGTFFLQDSKELTEPIVKVGDKV
                     NKGDIIGYVEAMKVLNEVTTDVAGEITEIVADHGTNVEYDQVLVRIK"
     gene            complement(3929..4939)
                     /locus_tag="NBH32_000758"
     CDS             complement(3929..4939)
                     /locus_tag="NBH32_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000023326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD1514385.1"
                     /translation="MSIKILQPGLFSTVQDLGRKGYEHIGFSGSGAMDQFSFKVAQSL
                     INNDGPAIEYTLIGPTIQFNTQNTFVITGGSVNASLNNKTISMNSVILAEKGDILKIG
                     AITKGARGYLTFGHSINVPSIAESYATHTRSSIGGFKGRKLLANDVITVKINNDFKEN
                     IGKTIHLQDDLLPENNIIHILQGPQFEAFSEEARAKIVNYPYLITEQSDRMGYRLEGD
                     SVAPINQADIISEPVALGSVQVPNDGQPIILLNDKQTIGGYTKIATVCKFDLPKLAQM
                     KPQDTIQFKWISFQEAVDKNREQMSLFNEILKSHQKTPIFDTSSLRHTSKKLATILKG
                     DL"
     gene            complement(4929..5663)
                     /gene="pxpB"
                     /locus_tag="NBH32_000759"
     CDS             complement(4929..5663)
                     /gene="pxpB"
                     /locus_tag="NBH32_000759"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001617363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCD1514386.1"
                     /translation="MDVRFINEQTIMIYFENNISEETYRNVTAMVRWIREKEILEIQD
                     IVPSYRAVLIYFDEQAITSSKLIENLELNKFNEKNVHAVNQTNRIIKIPVQYGGTYGP
                     DIEEVAKHNRITVEQVIEKHTSKPYLIYMLGFMPGFPYLGGLDQQLHTPRRNQPRLKI
                     HAGSVGIANNQTGLYPSDSPGGWQIIGRTPLKVFSSEREPMSMYEAGEWIQFYAIDEQ
                     EFIQIERDISDGNFNVDDWVVIENVN"
     gene            complement(5990..6466)
                     /gene="greA"
                     /locus_tag="NBH32_000760"
     CDS             complement(5990..6466)
                     /gene="greA"
                     /locus_tag="NBH32_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCD1514387.1"
                     /translation="MENQKQYPMTQEGFEKLERELEELKTVKRPEVVEKIKVARSFGD
                     LSENSEYDAAKDEQGFIEQDIQRIEHMLRNALIIEDTGDNNVVKIGKTVTFVELPGDE
                     EESYQIVGSAESDAFNGKISNESPMAKALIGKGLDDEVRVPLPNGGEMNVKIVNIQ"
     gene            complement(6494..7117)
                     /gene="udk"
                     /locus_tag="NBH32_000761"
     CDS             complement(6494..7117)
                     /gene="udk"
                     /locus_tag="NBH32_000761"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCD1514388.1"
                     /translation="MKATTIIGIAGGSGSGKTTVTNEIMKNLEGHSVALLAQDYYYKD
                     QKHLTFDERLETNYDHPFAFDNDLLIENLKDLKNGKAVEVPTYDYASHTRSDITIDFK
                     PKDVIIVEGIFALENKVLRDMMDVKIYVDTDADLRILRRLTRDTKERGRSMDSVINQY
                     LSVVRPMHDQFIEPTKKYADIIIPEGGSNKVAIDIMTTKIQSLVSKQ"
     gene            complement(7117..8385)
                     /locus_tag="NBH32_000762"
     CDS             complement(7117..8385)
                     /locus_tag="NBH32_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1514389.1"
                     /translation="MKTIEEIKSTPKTVMKKPELLAPAGNLEKLKIAVHYGADAVFLG
                     GQEYGLRSNADNFTMEEIAEGVEFANRYGAKIYVTTNIIAHDENIEGLESYLRNLEKT
                     GATGIIVADPLIIETCKEVAPKLEIHLSTQQSLSNYKAVEYWKEEGLDRVVLARETGA
                     MEMREMKEKVDIEIEAFIHGAMCIAYSGRCTLSNHMTARDSNRGGCCQSCRWDYELLE
                     VDDNGELDVFYNQGEVTPFAMSPKDLKLIESIPQMMDIGVDSLKIEGRMKSIHYIATV
                     VSVYRKVIDAYAADPDNFKINPEWLIELDKCANRDTAPAFFEGTPGYEEQMFGQQQSK
                     KSPFDFCGLVLDYNEDTKIATIQQRNNFKPGQEIEFFGPEIETFTQVVEAIYDEEGNS
                     LDAARHPLQIVQIKVDRPIYPNNMMRKEIG"
     gene            complement(8397..9320)
                     /locus_tag="NBH32_000763"
     CDS             complement(8397..9320)
                     /locus_tag="NBH32_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCD1514390.1"
                     /translation="MTELLVTPKSLSHMETLIDLGADAFVIGEQKFGLRLPGEFNRQQ
                     MTEAVALAHKNDKKVYAAVNGLFHNYHLDAVEDYINFLHEIRVDRIIFGDPAVVMYVK
                     AQENPIPLHWNAETLVTNHFQCNYWGKRGASRAVLARELNLDEIINIKENSNVEIEVQ
                     VQGMTCMFQSKRMLLGNYYTFQDRQMKIERRNDEQSLLLYDEERQNNYPVYEDYNGTH
                     IMSPNDICLIEELAPFFEAGIDSFKIDGILQTEEYINVVTEQYRQAIDLYNEDPEIYE
                     DEKFMLMDPIEEIQPDHRPFDEGFLYKQTVY"
     gene            complement(9323..9961)
                     /locus_tag="NBH32_000764"
     CDS             complement(9323..9961)
                     /locus_tag="NBH32_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HCD1514391.1"
                     /translation="MDDLNKKYLIDLHQHQNSSIEVLREFAEVNEVPIVDRLTLDLIK
                     QLIRMNNVKNILEIGTAIGYSSMQFASISDDIHVTTIERNETMIQYAKQNLATYHFEN
                     QVRIIEGNALEQFENVNDKVYDMIFIDAAKAQSKKFFEIYTPLLKHQGLVITDNVLYH
                     GFVSDIGIVRSRNVRQMVKKVQDYNEWLIKQPGYTTNFLNIDDGLAISIKGE"
     gene            complement(10246..10554)
                     /locus_tag="NBH32_000765"
     CDS             complement(10246..10554)
                     /locus_tag="NBH32_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HCD1514392.1"
                     /translation="MTEHNHDSQLEINNEEELLTLFDEEGNEVLYRKVLEFYHPEFKK
                     EYVILAEEGAQSDEDDMIELVPMINEPDESGDGGKLVPIETDEEWDMIEEVVNTEMEE
                     "
     gene            complement(10569..10997)
                     /gene="ruvX"
                     /locus_tag="NBH32_000766"
     CDS             complement(10569..10997)
                     /gene="ruvX"
                     /locus_tag="NBH32_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCD1514393.1"
                     /translation="MLQHKILGLDVGSRTVGIAISDIMGWTAQGLDTLRINEENNELG
                     IDQLVDIIKKHNVGTVVIGLPKNMNNSIGFRGEASLTYKEKLLEAYPSIEIVMWDERL
                     STMAAERSLLEADVSRQKRKQVIDKMAAVFILQGYLDSLH"
     gene            complement(11001..11261)
                     /locus_tag="NBH32_000767"
     CDS             complement(11001..11261)
                     /locus_tag="NBH32_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HCD1514394.1"
                     /translation="MENFDKTMKFDYEELPTQDVRDVLNNVYRTLDERGYNAVNQIVG
                     YLLSGDPAYIPRQNEARNQIRHIDRDVIMEELVSYYLKEQNK"
     gene            complement(11324..13954)
                     /gene="alaS"
                     /locus_tag="NBH32_000768"
     CDS             complement(11324..13954)
                     /gene="alaS"
                     /locus_tag="NBH32_000768"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCD1514395.1"
                     /translation="MKKLKASEIRQKYLDFFVEKGHMVEPSAPLVPIDDDTLLWINSG
                     VATLKKYFDGRETPKKPRIVNSQKAIRTNDIENVGFTARHHTFFEMLGNFSIGDYFKQ
                     EAIEFAWEFLTSDKWMGMEPDKLYVTIHPEDMEAYNIWHKDIGLEESRIIRIEGNFWD
                     IGEGPSGPNTEIFYDRGEAYGQDDPAEEMYPGGENERYLEVWNLVFSEFNHNKDHSYT
                     PLPNKNIDTGMGLERMASVSQNVRTNYETDLFMPIMNEIEKVSGKQYLVNSEQDVAFK
                     VIADHIRTIAFAISDGALPANEGRGYVLRRLLRRAVRFSQTLGINEPFMYKLVDIVAD
                     IMEPYYPNVKEKADFIKRVIKSEEERFHETLEDGLAILNELIKKAKATTNEINGKDAF
                     KLYDTYGFPIELTEEIAVQAGLKVDMTTFESEMQQQRDRARQARQNSQSMQVQSEVLK
                     NITSASTFVGYDTATAQTTLTHLIYNGEEVSQVEAGETVYFMLTETPFYAVSGGQVAD
                     TGIVYNGNFEIAVSEVTKAPNGQNLHKGVVQFGQVNVGATVSAEVNQNDRRDIQKNHS
                     ATHLLHAALKSVLGDHVNQAGSLVEADRLRFDFSHFGPMTKDEIDQVERLVNEEIWKG
                     IDVNIQEMDIASAKEMGAMALFGEKYGDVVRVVNMAPFSIELCGGIHVRNTSEIGLFK
                     IVSESGTGAGVRRIEALTGKAAFLYLEDIQEKFNTMKSQLKVKSDDQVVDKLTQLQDE
                     EKALLKQLEQRDKEITSLKMGNIEDQVEEINGYKVLVTEVDVPNAKAIRSTMDDFKSK
                     LQDTIIILASNVDDKVSMVATVPKSLTNNVKAGDLIKQMAPIVGGKGGGRPDMAQGGG
                     TQPENISKSLSFIKDYIKNL"
     gene            complement(14297..16774)
                     /locus_tag="NBH32_000769"
     CDS             complement(14297..16774)
                     /locus_tag="NBH32_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HCD1514396.1"
                     /translation="MSDPTLFDYSMIKGTVEAILFQNSDNFYTVLKVDTIETNEDFDT
                     MPTVVGFLPNIVEGDVYTFKGQVVDHPRYGKQLKAETFEKEMPQTKEAIISYLSSDLF
                     KGVGKKTAQNIVNALGDNAINDILDDHSVLEKVSGLSKKKQKQIAEQISANQESEKIM
                     IRLHDLGFGPKLSMAIYQFYLGDTLTILDQNPYQLIYDIKGIGFNKADQLARNIGIAY
                     NDNERLKAALLYTLEEECIKQGHTYLPINVVIDLTVDVLNYQDEEVIEPEKLDEMLQY
                     LNEEKRLIIDNEQVAIPSLYYSEIKSVQNLFRIKTHTNKLTEIEQSDLQMHIGEIEDA
                     NQVNYAASQKEALQTAINSKVMLLTGGPGTGKTTVIKGIVELYAEIHGLSLDYDDYVN
                     DDYPVVLAAPTGRASKRLQESTGLEAMTIHRLIGWNQDTKPEDILENEINARLIIIDE
                     MSMVDTWLFHQFLSAVPLDAQLIFVGDEDQLPSVGPGQVFKDLIESKAIPRVNLTEVY
                     RQQDGSSIIELAHRMKLGQKIDITQRFHDRSFINCQANQIPTVVEKVVTSAVNKGYTM
                     ADIQVLAPMYKGNAGIKRLNQVLQDILNPKKKDTREIEFGDVVFRKGDKVLQLVNRPN
                     DNIFNGDIGVIVGIFWAKENALNKDVLVVDFEGNEITFTKQDMMELTHAYCTSIHKSQ
                     GSEFPIVIMPIVKQYFRMLQRPILYTGLTRAKTSLVLLGDPEAFDIGLKTNGQARLTQ
                     LCTLLKNYFNSEDEEMLENTATNDTGASQTTIDDQVEAPMPSNDSEEVTSDSTKTDTN
                     VLTEATMFKIDPMINMGEITPYDFIER"
     gene            complement(16776..17444)
                     /locus_tag="NBH32_000770"
     CDS             complement(16776..17444)
                     /locus_tag="NBH32_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCD1514397.1"
                     /translation="MIDQQTIYQYIQNGKIEEALQALFGNIEEDPTIIENYINAGIVL
                     ADANEIEKAERFFQKALTIDPKNGVVFYNLANVYYNQQRYQEAIKLYQQALQTEIEQV
                     DCNYMIGMAFNQLESFKLALPYLMTAAELDKDKDAEVQFQYGLVLCQLEMFNEAITQL
                     KHVLTIDKNHVDARYNLGLALFMKNEDIDEAITHFKEAVTIDPKHLLSQHALKTFTKM
                     KEEE"
     gene            complement(17961..19079)
                     /gene="mnmA"
                     /locus_tag="NBH32_000771"
     CDS             complement(17961..19079)
                     /gene="mnmA"
                     /locus_tag="NBH32_000771"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCD1514398.1"
                     /translation="MSNKDIRVVVGMSGGVDSSVTAHVLKEQGYDVIGIFMKNWDDTD
                     ENGVCTATEDYNDVIEVCNQIGIPYYAVNFEKEYWDKVFTYFLDEYKKGRTPNPDVMC
                     NKEIKFKAFLDHAMNLGADYVATGHYARIHRHEDGHVEMLRGVDNNKDQTYFLNQLSQ
                     QQLSKVMFPIGDIEKSEVRRIAEEQGLVTAKKKDSTGICFIGEKNFKTFLSQYLPAQP
                     GDMITLDGKKMGKHSGLMYYTIGQRHGLGIGGDGDPWFVVGKNLKDNVLYVEQGFHHD
                     ALYSDYLIASDYSFVNPEDNDLDQGFECTAKFRYRQKDTKVFVKRENDHALRVTFAEP
                     VRAITPGQAVVFYQGDVCLGGATIDDVFKNEGQLNYVV"
     gene            complement(19080..20222)
                     /locus_tag="NBH32_000772"
     CDS             complement(19080..20222)
                     /locus_tag="NBH32_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1514399.1"
                     /translation="MEIYADYAATTPVKPEVVDAMMTIYNSHYGNPSSIHAKGRDARK
                     YLDESRRQIAQLLGADTHEIIFTSGATESNNTAIKGIVKANEQLGNHIITSKIEHHSV
                     LHVFEQLEREGFDVTYLDVDDTGAIDLDQLEETITDKTILVSIMFVNNEVGTVQQIYD
                     IQDIIAETNAYFHVDAVQAIGHLDVKFDEFEIDAMSITAHKFGGPKGVGALLVKDHVT
                     LDYPQLGGEQELKRRAGTENLAQIVGMAKALQLAEKNRDDNNIHLMNLKEQFLVKLQE
                     RAIPFELNGSMTDATGHIVNLYFPFVEVETMLTLLDMAQIYVSSGSACTAGSTQPSHV
                     LDAMFEDEERSNHSIRFSFNELTTENEINAIVAEIHKIYFKFKEES"
     gene            20534..21547
                     /locus_tag="NBH32_000773"
     CDS             20534..21547
                     /locus_tag="NBH32_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCD1514400.1"
                     /translation="MAIKYSALNLVPIREGEDERTAINDMVKLAQHLDELSYERYWIA
                     EHHNAPNLVSSATALLIQHTLEHTKHIRVGSGGIMLPNHAPLIVAEQFGTMATLFPNR
                     VDLGLGRAPGTDMMTASALRRDQHDGVYKFPEEVSLLQQYFGPAHQQAYVRAYPAVGK
                     NVPLYILGSSTDSAHLAARKGLPYVFAGHFAPQQMKEAIEIYKTLFEPSDVLDEPYVI
                     VCLNTIVAENDEEAQYLASSMAQVMVSITRGRMQPVQPPTHDLQNILTPREYAMAMER
                     QKISLIGSENTVQQKIQDFMETYGEVNEIMAISYIYDKDMQLDSYRRFKNVINQINEK
                     NTL"
     gene            complement(21784..21930)
                     /locus_tag="NBH32_000774"
     CDS             complement(21784..21930)
                     /locus_tag="NBH32_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514401.1"
                     /translation="MSFMDKAKDAVEKFKNSDNEQVKNVKDKINEYTGSNNEEKKENE
                     DKEK"
     gene            complement(21970..22152)
                     /locus_tag="NBH32_000775"
     CDS             complement(21970..22152)
                     /locus_tag="NBH32_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1514402.1"
                     /translation="MADESKFDQFKGNVKETVGNVTDNKELEKEGQQDKATGKAKEVV
                     ENAKNKITDAIDKLKK"
     gene            complement(22252..22674)
                     /locus_tag="NBH32_000776"
     CDS             complement(22252..22674)
                     /locus_tag="NBH32_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD1514403.1"
                     /translation="MKISTKGRYGLTLMISLAKKEGQGCISLKSIAEENNLSDLYLEQ
                     LVGPLRNAGLIRSVRGAKGGYQLRVPAEEISAGDIIRLLEGPITFVESIESEPPAQKQ
                     LWIRMRDAVRDVLDNTTLKYLAEYVDTSEDLDGYMFYI"
     gene            22759..24033
                     /locus_tag="NBH32_000777"
     CDS             22759..24033
                     /locus_tag="NBH32_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HCD1514404.1"
                     /translation="MSTEPLASRMRPKNIDEIISQQHLVGPRGIIRRMVDTKKLTSMI
                     FYGPPGIGKTSIAKAISGSTQYKFRQLNAVTNTKKDMQLVVEEAKMSGQVILLLDEIH
                     RLDKAKQDFLLPHLENGKIVLIGATTSNPYHAINPAIRSRAQIFELYPLNDEDVRQAL
                     TRAIEDDENGLKTYQPKIDEDAMTYFSTQSQGDVRSALNALELAVLSADNDKDGYRHV
                     TLQDAKDCLQKGAFVSDKDGDMHYDVMSAFQKSIRGSDVNAALHYLARLIEAGDLPTI
                     VRRLLVISYEDIGLASPNAGQRTLAAIESAERLGLPEARIPLSQAVIELCLSPKSNSA
                     MSAIDSALSDIRNGHVGQIPNHLKDGHYQGAKDLGRSIGYKYPHQYVNGYVSQQYLPD
                     KLKNKIYYEPKTTSKSEQQLKEIYNNLLKQRP"
     gene            complement(24194..24967)
                     /locus_tag="NBH32_000778"
     CDS             complement(24194..24967)
                     /locus_tag="NBH32_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA threonylcarbamoyladenosine dehydratase"
                     /protein_id="HCD1514405.1"
                     /translation="MKHQFSRNELAIGQQGLDLLKQQTVVILGVGGVGSFAAEALART
                     NIGHIILIDKDDVDITNVNRQLHALTTTVGQSKVSLMEERIKLINPDCKVTPLHMFYT
                     EETYEEIFDNYDIDYFVDASDTIMYKVHLMKECLDRGIKVISSMGAANKTDPTRFTIS
                     DISKTHTDPMAKIIRQKLKKLGIRKGIPVVFSDESPIVIREDVKDIVGDKNAINRKGQ
                     MPPSSNAFVPSVVGLICASYVVNDVLKDIPVRRIKDKGQ"
     gene            complement(24967..25095)
                     /locus_tag="NBH32_000779"
     CDS             complement(24967..25095)
                     /locus_tag="NBH32_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514406.1"
                     /translation="MGLIAFNKCFFVEFLKNNQSNNAKYKILSYYVLMYKSVKEVK"
     gene            complement(25160..25350)
                     /gene="ssrS"
                     /locus_tag="NBH32_000780"
     ncRNA           complement(25160..25350)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="NBH32_000780"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(25428..27194)
                     /gene="aspS"
                     /locus_tag="NBH32_000781"
     CDS             complement(25428..27194)
                     /gene="aspS"
                     /locus_tag="NBH32_000781"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCD1514407.1"
                     /translation="MSKRTTYCGLVTEAFLGQEITLKGWVNNRRDLGGLIFVDLRDRE
                     GIVQVVFNPAFSEEALKIAETVRSEYVVEVQGTVTKRDPETVNPKIKTGQVEVQVTNI
                     KVINKSETPPFSINEENVNVDENIRLKYRYLDLRRQELAQTFKMRHQITRSIRQYLDD
                     EGFFDIETPVLTKSTPEGARDYLVPSRVHDGEFYALPQSPQLFKQLLMISGFDKYYQI
                     VKCFRDEDLRADRQPEFTQVDIEMSFVDQEDVMQMGEEMLKKVVKEVKGVEINGAFPR
                     MTYKEAMRRYGSDKPDTRFEMELIDVSQLGRDMDFKVFKDTVENDGEIKAIVAKGAAE
                     QYTRKDMDALTEFVNIYGAKGLAWVKVVEDGLTGPIGRFFETENVETLLTLTGAEAGD
                     LVMFVADKPNVVAQSLGALRVKLAKELGLIDETKLNFLWVTDWPLLEYDEDAKRYVAA
                     HHPFTSPKEADIAKLGTAPEEAEANAYDIVLNGYELGGGSIRIHDGELQEKMFEVLGF
                     TKEQAQEQFGFLLDAFKYGAPPHGGIALGLDRLVMLLTNRTNLRDTIAFPKTASATCL
                     LTNAPGEVSDKQLEELSLRIRH"
     gene            complement(27210..28472)
                     /locus_tag="NBH32_000782"
     CDS             complement(27210..28472)
                     /locus_tag="NBH32_000782"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCD1514408.1"
                     /translation="MIKIPRGTQDILPEDSKKWRYIENQLDELMTFYNYKEIRTPIFE
                     STDLFARGVGDSTDVVQKEMYTFKDKGDRSITLRPEGTAAVVRSYIEHKMQGNPNQPI
                     KLYYNGPMFRYERKQKGRYRQFNQFGVEAIGAENPSVDAEVLAMVMHIYQSFGLKHLK
                     LVINSVGDMASRKEYNEALVKHFEPVIHEFCSDCQSRLHTNPMRILDCKVDRDKEAIK
                     TAPRITDFLNEESKAYYEQVKAYLDDLGIPYIEDPNLVRGLDYYTHTAFELMMDNPNY
                     DGAITTLCGGGRYNGLLELLDGPSETGIGFALSIERLLLALEEEGIELDIEENLDLFI
                     VTMGDQADRYAVKLLNHLRHNGIKADKDYLQRKIKGQMKQADRLGAKFTIVIGDQELE
                     NNKIDVKNMTTGESETIELDALVEYFKK"
     gene            complement(28933..29808)
                     /locus_tag="NBH32_000783"
     CDS             complement(28933..29808)
                     /locus_tag="NBH32_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1514409.1"
                     /translation="MKKIEAWLSKKGLKNKRTLIVVIAFVLFIIFLFLLLNSNSEDSG
                     NITITENAELRTGPNAAYPVIYKVEKGDHFKKIGKVGKWIEVEDTSSNEKGWIAGWHT
                     NLDIVADNTKEKNPLQGKTIVLDPGHGGSDQGASSNTKYKSLEKDYTLKTAKELQRTL
                     EKEGATVKMTRTDDTYVSLENRDIKGDAYLSIHNDALESSNANGMTVYWYHDNQRALA
                     DTLDATIQKKGLLSNRGSRQENYQVLRQTKVPAVLLELGYISNPTDETMIKDQLHRQI
                     LEQAIVDGLKIYFSA"
     gene            complement(29805..30256)
                     /locus_tag="NBH32_000784"
                     /pseudo
     CDS             complement(29805..30256)
                     /locus_tag="NBH32_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500248.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
     gene            complement(30268..32457)
                     /locus_tag="NBH32_000785"
     CDS             complement(30268..32457)
                     /locus_tag="NBH32_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HCD1514410.1"
                     /translation="MNNEYPYSADEVLHKAKSYLSADEYEYVLKSYHIAYEAHKGQFR
                     KNGLPYIMHPIQVAGILTEMRLDGPTIVAGFLHDVIEDTPYTFEDVKEMFNEEVARIV
                     DGVTKLKKVKYRSKEEQQAENHRKLFIAIAKDVRVILVKLADRLHNMRTLKAMPREKQ
                     IRISRETLEIYAPLAHRLGINTIKWELEDTALRYIDNVQYFRIVNLMKKKRSEREAYI
                     ETAIDRICTEMDRMNIEGDINGRPKHIYSIYRKMMKQKKQFDQIFDLLAIRVIVNSIN
                     DCYAILGLVHTLWKPMPGRFKDYIAMPKQNLYQSLHTTVVGPNGDPLEIQIRTFDMHE
                     IAEHGVAAHWAYKEGKKVSEKDQTYQNKLNWLKELAEADHTSSDAQEFMETLKYDLQS
                     DKVYAFTPASDVIELPYGAVPIDFAYAIHSEVGNKMIGAKVNGKIVPIDYILQTGDIV
                     EIRTSKHSYGPSRDWLKIVKSSSAKGKIKSFFKKQDRSSNIEKGRMMVEAEIKEQGFR
                     VEDILTEKNIQVVNEKYNFANEDDLFAAVGFGGVTSLQIVNKLTERQRILDKQRALNE
                     AQEVTKSLPIKDNIITDSGVYVEGLENVLIKLSKCCNPIPGDDIVGYITKGHGIKVHR
                     TDCPNIKNETERLINVEWVKSKDATQKYQVDLEVTAYDRNGLLNEVLQAVSSTAGNLI
                     KVSGRSDIDKNAIINISVMVKNVNDVYRVVEKIKQLGDVYTVTRVWN"
BASE COUNT         9978 a         6399 c         4558 g        11755 t
ORIGIN      
        1 cttaaaatat attcaattat gctttataaa tgaatcaccg gaaatcaaaa tacatcttgc
       61 ctaatactaa caacatttac agtgatacta ttattcgaaa tgtcttttaa aataactgaa
      121 gtacttcttt gaatgagaat atacctgtca atattgtaac aagtactgca acgataccaa
      181 agatgaacat ccaatttggg tgtttataat caccaacaat tgatttcttt ttacttgcaa
      241 tcaaaattgc acctaacgta ataggtaaaa tccatccatt tatcgcgcct gctataatta
      301 aaaggctgat tggttttcca ataaacaaga aaatcattgt tgaaataacg ataaacacaa
      361 tgacaattaa attacttctt tcgtcaagtg atttatgaag tgtttttaaa aatgttgcgc
      421 ttgtgtatgc tgagccaatt actgatgaca tagctgcagc aaataacaca ataccaaaaa
      481 tattctttcc aattggtcca attgcgtgtt caaaaactga cgctggtgga ttttcagaac
      541 ttagtgtcac acctgttaca acaactccta ataccgctag gaatagtaac gttctcataa
      601 tacctgtagt taaaatacca gcaattgctg attgatttac aaatggtaaa tattgcttac
      661 ctttaatgcc agagtctaaa atacgatgtg cacctgcaaa ggtaatataa ccacctacag
      721 ttccaccaac taacgtaatg attggcaaga ctaatttcat tggatgttct ggcgcaaatg
      781 tatgcacaaa agcatcacca taaggtggat tagaaacaaa catcacatat gccacaacta
      841 aaatcatcac aataccaaga atcattgaaa caacgtccat aattttttgg ccacttttac
      901 ttacaaagat taatattgca aagattgcag taatagctgc gccccatttt acatctaatc
      961 caaaaattgc atttaaacct aaaccggcac cagcaatatt accaatatta aaagctagac
     1021 ctccaaatgc aatcaatatt gagataacag taccaagccc aggaacaact ttatttgata
     1081 tttcttgacc tcttaaacca gtgacaacca atatgcgcca tatattaatt tgtgcaccaa
     1141 tatcaataat gattgatagt aaaattgcga atgcaaaact cgccaaaaat tgtgatgtaa
     1201 atactgctgt ttgcgttaaa aatgctggcc caattgcaga agttgccatc agaaatacag
     1261 aacctaacaa taatctctta tggtttttag taaattcaaa atcactttca cgatgtttaa
     1321 gctttaaatt tctccccatt ttttagcccc ctataaggat tgaatatcaa tgccttcttt
     1381 cattaaaatt tctctaattt tcgaaacaaa taataatgca tgttctccat caccatgcac
     1441 acaaattgta tctgcttgta acgttacttc cttattgttt tttgaaataa ctttattttc
     1501 cttcaccatc tttaaaacct gcttaagtgc ttcgtcagta tcagtaatca cagcatcact
     1561 ttcttttcta ctaacgagct gcccatcatc ttcgtatcgt ctatcagcaa acacttcaga
     1621 agctgtaatt aatccgacat tctttgcttc tgaaattaga tatgaatttg ctaatcctac
     1681 taacactagt gatggatcaa agtcataaac agcttgtgct ataacgcttg ctatttctct
     1741 gtcttttgca cccatctgat acaatgcacc atgcggttta acatgattaa ttttaacttg
     1801 atgaatgcga caaaaccctt gtaatgcacc taattgataa atcatcaaat tataaatctc
     1861 gtcgttagag atatctatat ttcgtctgcc aaagcctttc aaatcaggta aaccaggatg
     1921 tgcacctact gcaacattat gtgctttggc aagttttacc gtttcattca ttacattttc
     1981 atcaccagcg tgaaaaccac aagcaacatt cgcacttgta attaacggaa taatttgatg
     2041 atcaccacca aaggaataat ttccaaatgc ttcgcctaaa tcacaattca aatcaactcg
     2101 cattataatt ccaccccttt aacaatttga tgtttttcta aaaatttaat atcaacatct
     2161 tttgcatctc catcacgata tagtggataa tttaaaactg catataaaaa atcggcagtt
     2221 gtagaaaatc catctatcac catttcatct aaggtggctt tcaacttatc aattgctgaa
     2281 gctctatcat gagattttac aattacttta gctactaaag aatcataata tggtgaaact
     2341 tgataaccgt gatatagtaa agaatcgact cgcacattaa agccttgagg taaatgtaac
     2401 gctgtcactt tacctggtgt tggttgaaat ttcttttcag gattttcggc atttattctc
     2461 gcttctatca catgaccatt aaattgaata tcgctttgtg agaaaggtaa atgattatgt
     2521 tccaataaat acagttgtgc tgcaaccaaa tcacgttctg ctcgcatctc tgtaacagta
     2581 tgttcaactt gaattcgagc attcatttca ataaagtaat gtgcggtatc agttactaaa
     2641 aattcaatcg tacctgcact tctataattt gctgcacgtg caactttaac agcatcatta
     2701 catatttgtt gtcgcctttc ttcagttaat gctgcacaag gagattcttc gattaatttt
     2761 tgatttttac gttgtacaga acaatcacgt tcccctaaat gtacataatt atcctgccca
     2821 tcccccataa cttgaacttc aacatgtttt gcaacaggta taaaagcctc aacataaaca
     2881 cgatcatcat caaagtattt ttttccttca cttttagctt ctttaaatgc cttttctaaa
     2941 tcttcagctt tctttacaat acgtatacct ttaccaccac cgccactggc agctttgata
     3001 acaactggat aaccgatgtc tttggcaagc ttctcaattt cagacacatg attcacagca
     3061 ccatttgatc ctggaatcac aggaacacct gcatgatgaa ctgtttgtct tgctgttatt
     3121 ttatccccca tcatttccat cgttttttta gtaggcccta taaacgctat gccttgttcc
     3181 tcaacggttt gagcaaattt tgttgattct gataaaaatc catatcctgg gtgaattgca
     3241 ttagcaccag tgatttgtgc agcagatatg atgcggtcaa tatttaaata actatctaaa
     3301 gcattagctt ccccaataca tatagcttga tctgctaaat gtacatgcaa gctttgctcg
     3361 tcccgttttg cataaactgc tacagtttca atcccatatt ctctgcaagc tcttataatc
     3421 cttacagcaa tttcacctct gttcgcaatt aaacaacgaa gcatttactt acccccttta
     3481 cttaatacgt accaaaactt ggtcgtattc aacatttgtg ccgtgatcag ctactatttc
     3541 agtaatttct ccagcaacat ctgttgttac ctcgtttaat actttcatcg cttcaacata
     3601 tcctataata tctcccttgt taactttgtc accgactttc acaattggtt cagttaattc
     3661 tttactatct tgtaaaaaga atgtacctac cattggtgat ttaatgtcat gataatcatt
     3721 tgtcgaaaca tcggagttat cattcgcttt tgaagctgtc aaatcattat tgttcatact
     3781 ttgatttgat tgattactgt gtgcagccaa atgattcgag tcagtgaagt taatttctat
     3841 ttcatcttca aaatttttat atttaaattt cttaacatca ttttccttca ctaatttgat
     3901 tatttgttcg attttttcaa tattcatttt acaaatcccc ttttaaaatt gttgctaatt
     3961 ttttcgaagt atgtcgcaag ctagatgtat caaaaattgg agtcttttga tgactcttaa
     4021 gaatttcatt aaacagagac atttgttccc gattcttatc tacagcttct tggaatgata
     4081 tccatttaaa ttgaattgta tcttgtggtt tcatctgtgc taactttggc agatcaaatt
     4141 tgcatacagt tgcaattttg gtataaccac ctatcgtttg tttatcatta agcagaataa
     4201 taggttgacc atcatttggt acttgaacac tgccaagagc aaccggttca gaaatgatat
     4261 ctgcttgatt aattggtgca acgctgtcac cttccaaacg ataccccata cggtctgatt
     4321 gttcagttat taaatatgga taatttacaa ttttcgctct agcctcttca gaaaatgcct
     4381 cgaattgagg tccttgaaga atgtgtataa tattattttc tggtaataaa tcgtcctgta
     4441 aatgaatagt ctttccaatg ttttctttaa agtcattatt tattttcact gttattacgt
     4501 cattagctaa taactttcta cctttgaatc ctcctatact gcttcgggta tgtgttgcat
     4561 aactttcagc aatagaaggt acgttgatag aatgaccaaa agtaagataa ccgcgtgcac
     4621 ctttggttat agcacctatt tttaaaatgt cacctttctc agctaatatg acagaattca
     4681 ttgatatagt tttattattt agcgaggcat taacactacc accggttata acaaatgtat
     4741 tttgcgtatt aaattgaatg gtaggaccaa tcaaagtata ttcaatcgct ggaccatcat
     4801 tgttaattaa tgactgcgca accttaaaac taaattgatc catggcacct gagcctgaaa
     4861 atccaatatg ttcataacct tttcttccta gatcttgtac cgttgaaaag agacctggtt
     4921 gtaaaatctt aattgacatt ttcaatcacc acccagtcat caacattaaa gttgccatct
     4981 gatatatctc tttcgatttg tataaattcc tgttcatcta ttgcataaaa ttgtatccat
     5041 tctcctgctt cgtacattga cattggttca cgctcgctgc taaatacttt taacggtgtg
     5101 cgtccaataa tttgccatcc gccaggagaa tctgatggat atagtcctgt ttgattattc
     5161 gcaataccta cagaacctgc atgaattttt aaccttggct gattacgtct aggtgtatgt
     5221 agttgttgat caagtccgcc taagtatgga aatccaggca taaatcctag catatatatt
     5281 aaataaggtt tacttgtatg tttttcaata acttgctcaa cagttattcg attatgcttt
     5341 gctacttcct caatatcagg tccatatgta ccaccatatt gaacaggtat tttaataatt
     5401 cgattggttt gattcacagc atgaacattt ttttcattaa atttgttaag ttctaaattt
     5461 tcaattaatt tagaagatgt tatagcttgt tcatcaaaat atattagaac tgctcgatac
     5521 gaagggacaa tgtcttgaat ttctaatatt tctttttctc gtatccaccg taccattgct
     5581 gtgacatttc gatatgtctc ttcggatata ttattttcaa aataaatcat aattgtctgc
     5641 tcgttaataa atcttacatc cactctaaat ccccctttgt attgcaataa accagtattg
     5701 aatacctttt cattgtatca ttgagaagca caagttgttt aataagtaat tcaaatcgca
     5761 tacaataaca aattataatc aatttgtttt cttggaaata ttttgctttt tttaaaatgg
     5821 agtagttaat attgaaactc ttacactaat ttaggttctg ctatcaatcg gtctgatgga
     5881 aaatttttac ttttcatctg tccgattttt tgattttgaa tataaaaaag cacgaccgaa
     5941 gtatcattaa cacacttcaa tcgcgcaatt aaacaatcta tttgatcatt tattggatat
     6001 taacaatttt tacgttcatt tcgccaccat taggtagtgg aacacgaact tcatcatcta
     6061 aacctttacc aattaacgct ttagccattg gtgattcatt tgaaatctta ccattaaatg
     6121 catctgattc agctgaacca acgatttgat aactttcctc ttcatcacct ggtaattcta
     6181 caaacgttac tgttttacca attttaacaa cgttgttatc tccagtatct tcaatgatta
     6241 atgcatttct taacatatgc tcaattcttt gaatatcttg ttcgatgaat ccttgttcat
     6301 cttttgctgc atcatactca gagttctctg ataagtcacc aaatgaacgt gcaactttaa
     6361 ttttctctac aacttcagga cgcttaactg tttttaattc ttcaagttca cgctctaatt
     6421 tttcaaaacc ttcttgagtc attggatatt gcttttgatt ttccatattg tcatcttcct
     6481 ttactgaatt atactattgc ttgctaacta aagactgaat ttttgttgtc ataatatcta
     6541 ttgcaacttt attgctccca ccttcaggta taattatatc agcatatttc ttagtcggtt
     6601 caataaattg gtcatgcata ggtctaacaa cacttaaata ttgattgata acagagtcca
     6661 ttgaacgccc acgctcttta gtatctcgtg ttaaacggcg taatattctt aagtctgcat
     6721 ctgtatcaac atatatttta acatccatca tatcacgtaa taccttattt tctaaagcga
     6781 aaataccttc tacgataata acatctttag gtttaaaatc aatggtaata tcacttcttg
     6841 tatgactagc ataatcatat gtcggtactt ctactgcttt accatttttc aagtctttaa
     6901 gattttcaat taataaatca ttatcaaatg caaatggatg gtcataattg gtttctaggc
     6961 gctcgtcgaa agtcaagtgc ttttgatctt tataatagta atcttgagca agtaaagcga
     7021 cactatgacc ttctaagttt ttcataattt cgttagttac agttgttttt cctgagccag
     7081 atccaccagc tatgccaatg attgtagtag ctttcattag ccaatttcct ttctcatcat
     7141 gttgtttgga tatatcgggc gatccacttt aatttgaacg atttgtaatg gatggcgcgc
     7201 cgcgtctaag ctgttacctt cttcatcata aattgcttct actacttgtg taaatgtttc
     7261 aatttctgga ccaaagaatt ctatttcttg acctggttta aagttatttc gttgttgaat
     7321 agtcgcaatt tttgtatctt cattataatc taataccaaa ccacaaaaat caaatggtga
     7381 ttttttagat tgttgttgac caaacatctg ttcttcataa ccaggtgtac cctcaaagaa
     7441 tgctggtgca gtgtctctat ttgcacattt atctaactca attaaccatt ccggattaat
     7501 cttaaagttg tcaggatctg ccgcatacgc atcaatgact ttacgatata ctgagacaac
     7561 tgttgcaata taatgaattg acttcatacg tccttcaatt tttaatgagt ccacaccaat
     7621 atccatcatt tgaggaattg attcgattaa ttttaaatct ttaggactca tcgcaaacgg
     7681 tgtaacttca ccttgattat aaaatacatc aagttcacca ttatcatcaa cttctaataa
     7741 ttcataatcc caacggcaac tttgacagca accgcctctg ttggaatccc ttgcagtcat
     7801 atgattactt aatgtacatc tacctgaata ggcgatacac atagcaccat gaataaatgc
     7861 ttcgatttca atatctactt tttccttcat ttcacgcatt tccatcgcgc cggtctcacg
     7921 tgctaataca acacgatcca atccttcttc tttccaatat tctacagctt tgtaattaga
     7981 aagtgattgt tgagtagata aatgaatttc aagttttggc gcaacttctt tacatgtttc
     8041 gataattaaa ggatctgcaa caatgatacc tgtcgcacca gtcttttcca aattacgcaa
     8101 atatgattct agaccttcaa tattctcatc atgtgcaata atatttgtcg taacataaat
     8161 tttggcaccg taacggttcg caaattcaac accttcagct atttcttcca tcgtgaaatt
     8221 atcagcattt gaacgtaatc catattcttg accacctaaa aatacggcat cagcgccata
     8281 atgtactgct atttttaact tttctaagtt tccagcaggt gctaataatt ctggtttctt
     8341 cataactgtt ttaggagttg atttaatctc ttctattgtc ttcatgatta acctccttaa
     8401 tataccgttt gtttatataa gaaaccttcg tcaaatggtc gatgatcagg ttgaatttct
     8461 tcaattgggt ccatcaacat aaatttctca tcttcataga tttcaggatc ttcattgtac
     8521 aaatctatcg cttgacgata ctgttccgtt accacattaa tatattcttc cgtttgtaga
     8581 ataccatcga ttttaaatga atctataccc gcctcaaaaa atggtgctaa ttcttcaatt
     8641 aaacaaatgt cgtttggtga cataatgtgc gtaccattgt aatcttcgta aactgggtaa
     8701 ttattttgtc tttcttcatc ataaagtaat aaagattgtt catcattgcg acgttcaatt
     8761 ttcatttggc gatcttggaa cgtataataa ttgcctagta gcatacgctt tgattggaac
     8821 atacaagtca ttccttgaac ttgcacctca atttccacat ttgaattttc ttttatatta
     8881 ataatttcat ccaaattcag ctcacgtgct aagacagctc ttgatgcgcc tcttttcccc
     8941 cagtaattac attgaaaatg attagttact aacgtctctg cattccaatg aagtggtatt
     9001 ggattctctt gcgccttcac atacattact actgctggat ccccgaaaat aattctgtca
     9061 actcgtattt catgtaaaaa attaatataa tcttctacag catctaaatg ataattatga
     9121 aataatccat tcaccgccgc atatactttt ttatcgtttt tgtgagctaa tgcgacagcc
     9181 tctgtcattt gttgtctatt gaattcccct ggaagtctta aaccaaactt ttgctcgcca
     9241 attacaaaag catctgcacc taaatcaata agtgtttcca tatggcttaa tgacttgggt
     9301 gtgacaagta attctgtcat agtcattctc ctttaattga aatcgctaat ccatcgtcta
     9361 tatttaaaaa attcgttgta tatcctggtt gctttattaa ccactcatta taatcttgaa
     9421 cctttttaac catttgtctt acatttctcg atctaacaat cccaatatcc gatacaaaac
     9481 cgtgatataa aacattatct gtaattacga gaccttggtg ctttaaaagt ggtgtatata
     9541 tttcaaaaaa tttctttgat tgcgcttttg ctgcatcaat aaatatcata tcataaactt
     9601 tgtcatttac attttcaaat tgctctaaag cattaccttc aataattcga acctggtttt
     9661 caaaatgata agtagctaaa ttttgtttag catattgaat catcgtttca ttacgctcta
     9721 tcgttgtgac atgaatgtca tcagatatag aagcgaattg catagaacta tagccgattg
     9781 ctgtaccaat ttctaaaata tttttaacat tattcatacg aattaattgc ttaattaaat
     9841 ctaatgttaa acgatctacg attggcactt catttacttc ggcaaattca cgcaaaactt
     9901 cgattgaact attttgatgt tgatgtaaat ctattaaata ttttttattt aggtcatcca
     9961 tgttttaaac ttcctttatg taaaataagt caatatgatt atgacaataa aataaatcag
    10021 ccttcacaat tgattatgat tttgccaacc aattaaatga ctgatttcgt gttagacgca
    10081 aagctatttt atttatagaa gcgaatcatt catataaaat ttaactttag atattttacc
    10141 atattttcaa taaaattata agcgttaatt atttatacat tgcttgactt aaaaaaatac
    10201 tcttgcctcc ccatcttaag gttagcaaga gtaaaatctt tttaattatt cttccatttc
    10261 agtatttaca acttcttcaa tcatgtccca ttcttcatca gtttcgattg gtactaactt
    10321 accaccgtca cctgactcat ctggttcatt gatcattggt acaagctcaa tcatatcgtc
    10381 ttcatctgat tgagcacctt cttcagctaa gataacatac tcttttttga attcaggatg
    10441 ataaaattct aaaacttttc ggtataaaac ttcatttccc tcttcatcga ataaagttaa
    10501 taattcttct tcgttattaa tttctagttg tgaatcatga ttatgttcag tcatagtaaa
    10561 atctcctttt aatgtagtga atctaaatag ccttgtaaaa taaataccgc tgccatttta
    10621 tcaatcactt gttttctttt ttgtcttgaa acatctgctt ctaataatga tcgttcagca
    10681 gccattgtgc ttaatctttc atcccacatc acaatctcaa tagaaggata agcttctaat
    10741 aatttttctt tatatgttaa cgaagcttcg cctcgaaatc ctattgaatt attcatgttt
    10801 ttaggtagtc ctattacgac tgtacccaca ttatgttttt taataatgtc tactaattgg
    10861 tcaataccta attcattatt ttcttcattg attcggagtg tgtctaatcc ttgtgccgtc
    10921 caacccatta tatcactaat tgcaattcct accgttctac taccgacatc gagtcctaaa
    10981 attttatgtt gtaacataaa ttatttattt tgctctttta aatagtaaga aacaagctct
    11041 tccataataa catctctatc aatatgacga atttgatttc ttgcttcatt ttggcgtgga
    11101 atatacgcag ggtcacctga taataaataa cctacaattt ggtttacggc attatatcct
    11161 cgttcatcta atgttcgata aacattattt aaaacatctc ttacatcttg tgttggaagt
    11221 tcttcatagt cgaatttcat tgttttatca aagttttcca tttgcgacac tcctttaatt
    11281 acaaatataa ctcactatca tcatacaata ttatggctat aaattataga tttttaatgt
    11341 aatctttaat aaagcttaat gattttgaga tattttcagg ttgtgtaccg ccaccttgag
    11401 ccatatctgg acgaccgcca cctttaccac caacgattgg tgccatttgt ttgataagat
    11461 caccggcttt aacgttattt gttaaagatt tagggacagt tgcaaccatc gatactttat
    11521 catcaacatt acttgcaaga atgataattg tatcttgtag tttagattta aaatcgtcca
    11581 ttgtcgagcg aattgctttc gcatttggta catccacttc agtaaccaat actttatagc
    11641 cattgatttc ttcaacttga tcttcaatat tacccatttt aagtgatgta atttctttgt
    11701 cacgttgctc taattgtttt aataatgctt tttcttcatc ttgtaattgt gttaacttat
    11761 cgactacttg atcatcagat ttcactttca gctgtgattt catcgtatta aatttctctt
    11821 gaatatcttc taaatataag aaagctgctt tacctgttaa tgcttcaata cgacgcacac
    11881 cagctcctgt acctgactca cttactattt tgaataagcc aatttcagaa gtattgcgga
    11941 catgaatacc accacataat tcaattgaaa atggtgccat atttactaca cgcacaacat
    12001 caccatattt ttcaccgaat aatgccattg cgcccatttc tttagctgaa gcaatatcca
    12061 tttcttgaat gttaacgtca atacctttcc aaatttcttc atttactaag cgttcaactt
    12121 gatcaatttc atccttagtc attggaccaa aatgagagaa atcaaaacgt aaacgatctg
    12181 cttctactag tgaaccagct tggttaacat gatcacccag tactgatttc aacgctgcat
    12241 gtaataaatg tgttgcacta tggttctttt gaatgtcacg tcgatcattt tggttcactt
    12301 cagcagacac tgtagcgcca acatttactt ggccaaattg tactactcct ttatgcaagt
    12361 tttgaccatt tggtgctttg gttacttcac taacagcaat ttcaaaattg ccattataaa
    12421 caatacctgt atccgcaact tgtccaccac tgactgcata aaatggtgtt tccgttaaca
    12481 tgaagtatac tgtttcaccc gcttcaactt gtgaaacttc ttcgccattg tatatcaagt
    12541 gtgttagtgt tgtttgagct gtcgcagtat cataaccaac aaaagtactt gcagatgtaa
    12601 tatttttcaa tacttcactt tgaacttgca ttgattgaga attttgacgt gcttgacgtg
    12661 cacgatcacg ttgttgttgc atttctgact cgaatgttgt catatcaact ttcaatcctg
    12721 cttgcactgc tatttcttca gttaattcaa ttgggaaccc gtacgtatca tacaatttaa
    12781 atgcatcttt cccattaatt tcatttgttg tcgctttagc ttttttaatt aattcattta
    12841 aaatcgctaa gccatcttct aatgtttcat ggaatcgttc ttcttcagac tttataacac
    12901 gcttaatgaa atctgctttt tccttaacat ttggataata tggttccata atgtctgcaa
    12961 caatatcaac aagtttgtac ataaatggct cattgattcc taacgtttga ctaaaacgaa
    13021 cggcacgacg taacaatcga cgtaatacat atcctctacc ttcattggca ggtaatgcac
    13081 catcagaaat tgcaaatgca atcgtacgaa tgtggtcagc aattacttta aatgccacat
    13141 cctgttcgct gtttactaaa tattgtttac ctgatacttt ttcgatttca ttcattatag
    13201 gcataaataa atctgtttca tagttagtac gtacattttg agaaactgag gccatacgct
    13261 caagccccat gccagtatca atgtttttat taggtaatgg tgtgtaacta tgatctttat
    13321 tatgattgaa ttcactaaat actaagttcc atacttcaag atagcgttca ttttctccac
    13381 ctggatacat ttcttctgcc ggatcgtctt gtccatatgc ttctccgcga tcatagaaaa
    13441 tctcagtgtt cggtcctgaa ggcccttcac caatatccca gaagttacct tcaatgcgaa
    13501 taatacgact ttcttcaagc ccaatatctt tatgccaaat gttgtatgct tccatatctt
    13561 ccggatgaat cgtaacgtac aatttatctg gctccattcc catccattta tcactcgtta
    13621 aaaattccca agcaaattca atcgcttctt gtttaaaata atcaccaatt gagaagttac
    13681 ctaacatttc aaagaatgta tggtgacgcg ctgtgaaacc aacattttca atatcatttg
    13741 tacgaatagc tttttgagag tttacaattc ttggcttttt aggtgtttca cgtccatcaa
    13801 aatatttctt taatgttgct acacctgaat taatccataa taatgtatca tcatcaattg
    13861 gcactaatgg tgcagaaggt tcaaccatat gtcctttttc aacaaagaaa tctagatatt
    13921 tttgtctaat ttcactcgct tttaactttt tcatcattta cacatcctat ttactgtttt
    13981 taaattacca ttccataaaa attgatgaca cagatagtcg atttgaaaaa ctagtataaa
    14041 tcaatatcat tttttattat taaaaaataa aaaacgccca tcctcaaaag ggacgaacgt
    14101 tatcgcggta ccaccctagt tataaatgca attcaacaca tttatcactt taaatcgact
    14161 atacagttgt gcataaagta gcgttcacta atgtttgttg tacttttcac caaccagtac
    14221 atctctgata aacaaatcat taactactca tctttatacg aatttaattc tattttagtt
    14281 acatttacgc ttgttgtcaa cgttctataa agtcatacgg cgtgatttct cccatattaa
    14341 tcattgggtc aattttaaac attgtagctt ccgttaatac atttgtatct gtttttgttg
    14401 aatcagacgt aacttcttca ctatcattcg atggcattgg cgcttctact tgatcatcta
    14461 ttgtcgtttg tgaagctcct gtatcattag ttgctgtgtt ttctagcatt tcttcatctt
    14521 ctgaattaaa ataatttttc aacaatgtac ataattgtgt taaacgcgct tgaccatttg
    14581 ttttcaatcc aatatcaaat gcttctggat caccaagtaa aactaaactc gttttcgctc
    14641 tagttaaacc agtatataat atcggtcttt gtaacattct aaaatactgt ttaacaatag
    14701 gcatgataac aataggaaat tctgaacctt gtgatttatg gattgatgta caataagcat
    14761 gcgttaattc catcatatct tgtttcgtaa atgtaatttc attaccttcg aaatccacaa
    14821 caagtacatc tttattaagg gcattttctt tcgcccaaaa aataccaaca ataactccta
    14881 tgtcaccatt gaatatgtta tcatttggcc tattaacaag ttgtaatact ttgtcacctt
    14941 ttctaaagac tacatcacca aactcaattt ctcgtgtgtc tttcttttta gggtttaaaa
    15001 tatcttgtaa aacttgattt aaacgtttaa taccggcatt tcctttatac attggtgcaa
    15061 gcacttgaat atcagccata gtataccctt tattaacagc actagtaact accttctcaa
    15121 caactgttgg tatttggttt gcctgacagt taataaaact tctatcatga aaacgctgtg
    15181 taatatcaat tttctgaccc aatttcattc gatgtgctaa ttctataatg cttgaaccat
    15241 cttgttgacg atatacttca gtcagattaa ctcgtggtat agctttcgat tcaattaaat
    15301 ctttaaatac ttgaccagga cctacagaag gcaattggtc ctcatcacct acaaatatca
    15361 attgtgcatc taaaggaact gcacttaaaa attggtgaaa caaccaagta tctaccatag
    15421 acatctcatc aatgattatg agtcgtgcgt ttatttcatt ttctaatata tcctctggct
    15481 ttgtgtcttg attccaacct attaaacgat gaatcgtcat agcttctaat ccagttgact
    15541 cttgtagtct cttggacgct cttcctgttg gcgctgctaa tacaactgga taatcatcat
    15601 tgacataatc atcataatct aatgataagc catgaatctc agcatataat tctacaatgc
    15661 ctttaattac tgtcgttttt ccagttcccg gtccaccggt taatagcatc accttagaat
    15721 tgatagccgt ttgcaaagct tctttttgtg aagctgcata gttcacttga ttcgcatctt
    15781 ctatttcacc aatatgcatt tgtaaatctg actgttcaat ttctgtaagt ttatttgtat
    15841 gcgtctttat tctgaataag ttttgaacac ttttgatttc agaataatac aaacttggaa
    15901 ttgcaacttg ttcattgtca ataattagtc gtttttcctc atttaagtat tgcaacattt
    15961 cgtctaattt ttcaggttcg atgacctctt catcttgata atttaataca tcaaccgtta
    16021 aatctataac aacattgata ggcaaatatg tatgtccctg tttaatacat tcttcttcta
    16081 acgtatagag caacgcagct tttaatcgtt cattatcgtt ataagcgata ccaatatttc
    16141 tagcaagttg atctgcttta ttaaaaccaa tacctttaat atcataaatc aattgatatg
    16201 gattttggtc taaaatagtc agtgtatcac cgagataaaa ctgataaatt gccattgaaa
    16261 gtttagggcc aaaccctaaa tcatgtaaac gaatcattat tttttcagat tcttggtttg
    16321 ctgaaatttg ttctgcaatt tgtttctgtt tctttttaga taatcccgat actttttcta
    16381 gcactgaatg gtcatctaag atatcattta tcgcattgtc acctaatgca ttaacaatat
    16441 tttgagctgt ctttttacct acacctttaa acaaatcact tgataaataa cttataattg
    16501 cttctttcgt ttgtggcatt tctttttcaa aagtctctgc ttttaattgt ttaccataac
    16561 gtggatgatc aacaacttgc cctttaaatg tgtagacatc gccttcaaca atattcggaa
    16621 gaaaccctac aacagttggc attgtatcaa agtcttcatt tgtttcaata gtatctactt
    16681 taagcactgt ataaaaatta tcactgtttt gaaacaatat cgcttcaaca gtacctttga
    16741 tcattgaata atcaaatagt gtagggtctg acatgctatt cctcctcttt cattttagtg
    16801 aatgttttca gcgcatgctg acttaataag tgtttagggt cgatagtcac agcttcttta
    16861 aaatgagtta tggcttcatc aatatcttca tttttcataa ataacgctaa gcccaaattg
    16921 tatcttgcat caacatgatt tttatcaatc gttaatacat gtttaagttg agttatggct
    16981 tcattaaaca tttctaattg acataataca agaccatatt gaaattgaac ttctgcatct
    17041 ttgtctttat ctagttccgc agcagtcatt aaatacggca atgccagctt aaatgattct
    17101 aactgattaa acgccatacc gatcatataa ttacaatcaa cttgttcaat ctctgtttgt
    17161 aatgcttgtt gatataattt aatagcttct tgataacgct gctgattata atatacattt
    17221 gctagattat aaaatactac gccattcttc ggatctattg ttaaagcttt ttggaaaaag
    17281 cgctctgcct tttcaatctc attcgcatca gcaagtacga taccggcatt aatataattt
    17341 tcaataattg taggatcttc ttcgatattt ccgaacaatg cttgtaacgc ttcttctatt
    17401 tttccatttt gtatgtattg ataaattgtt tgttgatcta tcatttacga acctcatttc
    17461 tcatcaatta taacatcttg ataaattgta tgtctcgaat cacttaacaa cgaataaaat
    17521 ataatctaat atcatcttca ttcatgaaaa agcgggaatg gaatagaaat gctaaagaac
    17581 cattaacggt ttattatgta atggttcttc cacattagcc accactatta tgtacttaaa
    17641 aataggaata cataattaga ctcatgcata gggagtggga cagaaatgat atttttacaa
    17701 aattaatttg tttaccccaa cttccattgc cggttgaatt tctttacgaa attctctatg
    17761 ttggggcccc gccaacttcc attgcctgtt gaaattgggg ttccaatttc tctgtgttgg
    17821 ggccccgcca atataacatt gtagagccta ggacattgtg atgtcccaga ctctatcatc
    17881 atgaattatt ctcatcaaaa actgtctttc gtcatttcca acgctgaaac ttcaaataag
    17941 taatttattg ttgccattgt ttatacaaca taatttaatt gaccttcatt tttgaacaca
    18001 tcgtcaattg ttgctccacc aagacacaca tcaccttgat aaaaaacaac tgcttgtcca
    18061 ggtgtgattg ctcttactgg ctcagcaaaa gtaacacgta gtgcatggtc gttttcacgt
    18121 ttcacaaaaa ctttcgtatc tttttggcga tatctaaatt tagctgtaca ttcaaaacct
    18181 tgatctaagt cattatcttc tggatttaca aatgaatagt ctgaagcaat taagtaatca
    18241 ctgtataatg catcgtgatg gaatccttgt tctacatata aaacattatc ttttagattt
    18301 ttaccgacaa caaaccaagg atcgccatct ccacctatac ctaaaccatg tctttgtcct
    18361 attgtgtaat acatcaaccc actatgttta cccattttct taccatcaag tgttatcata
    18421 tcacccggtt gcgcaggtaa atattgagat aaaaatgttt taaagttttt ttcgccgata
    18481 aaacaaatac ctgtagaatc ttttttctta gcagtaacta gtccttgttc ttcagcaatt
    18541 cgacgcactt cactcttttc gatgtcgcca attgggaaca tcacttttga aagttgttgt
    18601 tgggataatt gattcaagaa gtatgtttga tctttattat tatctacacc acgtaacatt
    18661 tcaacatgac catcttcatg acgatgtatg cgtgcgtaat gccctgttgc tacataatct
    18721 gcacctaaat tcatcgcatg atctaaaaag gctttaaact taatttcttt attacacata
    18781 acgtctgggt ttggagtacg acctttttta tattcatcta agaaatacgt aaagacttta
    18841 tcccaatatt ctttttcaaa attaacagcg taatatggaa tgccaatttg attacacact
    18901 tcaataacat cgttgtaatc ttcagttgca gtacatacgc cattttcgtc agtgtcatcc
    18961 cagtttttca taaatatgcc aatgacatca taaccttgtt cttttaagac gtgagctgtt
    19021 acagaactat ctacaccgcc tgacatacca acgacaacac gtatatcttt atttgacaat
    19081 tatgactcct ccttaaattt aaaatatatt ttatgaattt cagctacaat tgcattaatt
    19141 tcattttcag tagtcaattc gttaaaacta aatcgaatcg aatgatttga tcgctcttca
    19201 tcttcgaaca ttgcatctaa aacatgcgac ggttgtgtag agcctgctgt acatgcagat
    19261 ccagacgaca catagatttg tgccatatcc aacaatgtta acatcgtttc aacttcaaca
    19321 aacggaaaat atagatttac aatatggcct gtagcatccg tcattgaacc atttaattca
    19381 aatggaatcg ctctttcttg taatttaact aaaaattgtt cttttaaatt cattaaatga
    19441 atattgttat cgtctcgatt cttttctgct aattgtaatg ctttagccat cccgacaatt
    19501 tgtgcaagat tttcagtacc tgcacggcgt ttcaattctt gttcaccgcc aagttgagga
    19561 taatctaatg tgacatggtc tttaactaat aatgcaccga caccttttgg tccgccaaac
    19621 ttatgagcag taatactcat tgcgtcgatc tcaaattcgt caaacttaac atcaagatgt
    19681 ccaattgctt gaaccgcatc aacatggaaa tatgcatttg tctcagcaat aatatcttga
    19741 atatcataaa tttgttgcac tgtgccaact tcattattta caaacataat agatactaaa
    19801 atcgtcttat ctgtaattgt ttcttcaagt tgatctaaat caatagcacc tgtatcatca
    19861 acatctagat atgttacatc aaaaccttct cgctctaatt gttcaaaaac atgtaacaca
    19921 gaatgatgtt caatcttcga tgtgataatg tgattaccca attgttcatt tgcttttact
    19981 atgcctttaa ttgccgtatt attcgattct gttgcaccac tcgtaaatat aatttcatgt
    20041 gtatctgcac caagtaattg tgcaatttga cgtcttgact catctaaata tttacgcgca
    20101 tctcttccct tagcatgtat tgatgatgga ttaccataat gcgaattgta aatcgtcatc
    20161 atcgcatcta ctacttcagg ttttactggt gtggtcgcag cataatctgc ataaatttcc
    20221 atgtttggac actcctcaca attttatcaa tgttccaata atagcacctt acatactatt
    20281 tttctacttt tctgtttaac tttatttata atgtttttaa ttatatttta ccattttcta
    20341 cacatgcttt tcgataggct tttttaagtt tatcgcttta ttcttgtctt ttttataaat
    20401 tttagtattt gcagatattt tttcattcgt aaaatgtaac gtactattat tttggttatg
    20461 agcaatttaa tatttatctg gttattcgat tggtatactt cttatatcat acaaaaggaa
    20521 ggacgataca aaaatggcga ttaaatattc agcattaaac cttgtcccaa ttcgagaagg
    20581 tgaagatgaa cgaacagcaa ttaatgatat ggttaagctc gcacaacatt tagacgaatt
    20641 atcatatgaa agatattgga ttgctgaaca ccataacgct cccaacctag taagttcagc
    20701 gactgcttta ttgattcaac atacgttaga acatacgaaa cacatacgtg taggttctgg
    20761 aggaatcatg ttacctaacc atgctccatt aatcgttgcg gaacaatttg gcacgatggc
    20821 aacattattt ccaaatcgtg tcgatttagg attaggacgt gcacctggaa cagatatgat
    20881 gaccgcaagt gcattaagac gagatcaaca tgatggtgtt tataaatttc cggaagaagt
    20941 ttcattatta caacaatatt tcggtcccgc tcaccaacaa gcatatgttc gtgcttatcc
    21001 agcagtaggt aaaaatgtgc ctttatatat tcttggttct tcaacagatt ctgcacattt
    21061 agctgctcgt aaagggcttc catatgtgtt cgctggacat tttgcacctc aacaaatgaa
    21121 agaagctatc gaaatttaca aaacgttatt tgaaccttct gatgtattag acgaacctta
    21181 tgttattgta tgtttaaaca caatcgttgc tgaaaatgat gaagaagcac aatatttagc
    21241 ttcatctatg gcacaagtta tggttagtat cactcgtggc agaatgcagc ccgttcaacc
    21301 gccaacacat gatctacaaa atatattaac gccgagagaa tatgcgatgg ctatggaaag
    21361 acagaaaata tcattaatag gttcagaaaa tactgttcaa caaaaaattc aagattttat
    21421 ggaaacttat ggtgaagtca acgaaattat ggcaataagt tatatttatg ataaagatat
    21481 gcaattagac tcttatcgtc ggttcaagaa tgttataaat cagataaatg aaaaaaacac
    21541 tttataatgt gataaataaa ctaagtgaaa gtatgtatcc ataatattaa taaaaataaa
    21601 cagtaacagc attttgaata aaagatgtct ttattgttca atcatttatt ttagtaatga
    21661 ttcaaattca cttaaaattc taatgcaaat atgaaagcgc cccttcaact ttacactgtg
    21721 ttagtgttta tttgatgggg cgctttcaaa atattgaaaa gcatatccaa aatttaaaga
    21781 aatttatttc tctttatctt cattttcttt tttctcttcg ttattcgatc ctgtatattc
    21841 atttatctta tcttttacat ttttaacttg ttcattatcg ctatttttaa atttttctac
    21901 tgcgtcttta gctttatcca taaaactcat attaatcgct cctcttatat ttaattagtt
    21961 taattgaact tattttttaa gtttatcaat tgcatcagtt attttgtttt tagcattttc
    22021 aacaacttct tttgctttac cagtcgcttt atcttgctga ccttcttttt ctaattcttt
    22081 gttatcagta acgttaccta ctgtttcttt aacatttcct ttaaattgat cgaacttact
    22141 ttcgtctgcc atagtgaaac ctccttggat gtatatattt atataccact aaggaggttc
    22201 gctaaacatt ataatatgaa gtttttatgt tatagtatag tatttatacg attaaatata
    22261 aaacatgtat ccgtctaaat cttcgcttgt atctacatat tccgctaaat atttcaatgt
    22321 tgtattatct aaaacatctc tcactgcatc tctcatgcga atccatagtt gtttttgcgc
    22381 aggtggttct gattcaatac tttcaacaaa tgtaattgga ccttccaaca gtcttataat
    22441 atcccctgct gagatttctt ccgctggcac tcttaattgg tatccacctt tagcaccgcg
    22501 tacacttcga attaaccccg catttcttaa aggacctaca agctgttcta aatataaatc
    22561 actcaaatta ttttcttcag caattgactt taatgatata catccttgcc cctctttttt
    22621 agcaagagaa atcatcaatg taagtccata tctcccttta gtagaaattt tcattgtata
    22681 acctcactta attcgaatat tgatattccc attttagcat tttttgagtt aagatagtat
    22741 aagaaaggtg tgacaaatgt gagtacagaa ccattagcat cgagaatgcg cccaaaaaat
    22801 atagatgaaa tcatttccca acaacattta gttggaccaa gaggcattat cagaagaatg
    22861 gttgatacaa aaaaattaac ttcaatgatt ttttatggtc cacctggtat aggcaaaaca
    22921 agtattgcca aagcaatttc gggcagtacg caatataaat tcagacaatt gaatgctgta
    22981 actaacacta aaaaagatat gcaacttgtt gttgaagaag ctaaaatgtc tggtcaagtt
    23041 atcttgttat tagatgaaat acatcgacta gataaagcta aacaagactt tttattacct
    23101 catttagaaa atggcaaaat cgtcttgatc ggtgctacaa cttcaaatcc ttatcatgct
    23161 atcaatccag cgattcgttc aagagcgcaa attttcgagt tatatccttt aaatgacgaa
    23221 gatgtgcgcc aagcgttaac tcgtgcaata gaagatgatg agaatggttt gaaaacatat
    23281 caacccaaaa ttgatgaaga tgccatgacc tacttttcta cacaaagcca aggtgatgtt
    23341 cgtagtgcgt taaatgcatt ggaattagct gtattaagcg cagataatga caaagacggt
    23401 tatcgacatg ttacattgca agatgctaaa gactgtttac aaaaaggtgc atttgtaagt
    23461 gataaggatg gtgacatgca ttacgatgtt atgagcgctt tccaaaaatc tatccgtggt
    23521 agcgacgtca atgccgcttt acattattta gcacgattaa ttgaagctgg agatttacct
    23581 acaatagttc gacgattact tgtaattagc tatgaggata taggcttagc ctcacctaat
    23641 gctggtcaga gaacacttgc tgctattgaa tcagcagaac gtctaggttt accagaagct
    23701 agaattccac taagccaagc agtaatcgaa ctatgcttat cacctaagtc aaattcagca
    23761 atgagtgcca ttgatagtgc attgtccgat attagaaacg gtcatgtggg ccaaattcca
    23821 aaccatttaa aagatggaca ttatcaaggt gctaaagatc taggccgatc tattggttac
    23881 aaatatccac accaatatgt taatggctat gtttcacagc aatatttacc tgataaactt
    23941 aaaaacaaaa tttattatga accaaaaacg acatctaaaa gtgaacaaca acttaaagaa
    24001 atatataaca acttacttaa acaaaggccg taaaaataag attaaatcga ttacctttga
    24061 tattttagct ataatcaaat caaaactgac tagtacacga ggcatattct actatgcttg
    24121 cgaactagtc agttttagtt ttatggtgcg gttacacttg ccttacgttg aaaattatga
    24181 ctttcaaaat taattattgc cctttgtctt taatgcgacg aactggaata tcttttaata
    24241 cgtcattcac cacataactt gcacaaatta atccaacaac acttggcaca aaggcatttg
    24301 aagaaggtgg catttgtcct tttcgattga tagcattttt atctccaact atatctttta
    24361 catcttctct tatgacaatt ggactttcat ctgaaaatac aactggaatc cctttacgaa
    24421 ttcctagttt tttcaatttt tgacgaataa ttttggccat tggatcggta tgtgttttag
    24481 agatatctga aattgtaaaa cgtgttggat ctgttttatt tgcagcaccc atactggaaa
    24541 tcactttaat ccctcggtca agacactctt tcattaagtg tactttgtac attattgtat
    24601 cacttgcatc tacaaaataa tctatatcgt aattatcgaa aatttcttca tatgtctctt
    24661 ctgtataaaa catatgtaaa ggtgtgactt tacaatctgg attaattaat ttaatacgtt
    24721 cttccatcaa agaaacttta ctttgtccta ccgttgtagt taaagcatgt aattgtctgt
    24781 ttacatttgt aatatcaaca tcatctttat caattaatat aatatgacca atattcgttc
    24841 ttgctaatgc ttcagcagca aatgaaccaa cacctccaac gccaagtatg acaacagttt
    24901 gttgcttcaa taaatctaaa ccttgttgtc caatcgctag ttcatttctt gaaaattgat
    24961 gtttcattat tttacctctt tcactgattt atacataagt acatagtaac ttaaaatttt
    25021 atatttagca ttattgcttt gattattttt caaaaattca acgaagaaac atttattaaa
    25081 cgctattaaa cccaactaat tctttattaa aaacttaaag aaacgcataa aaatacgcaa
    25141 gacaaagtct tgcgtatcga tagagtccgt attgccgtag ttataatagc ttgatcattc
    25201 ggcctgttat atacaggtgg gtgccctgtt tcttgttttg tacgtccttc atataaggcg
    25261 tgtacgctgc aagaaaaccc attgggctcc cttgatcaaa gagtgttagg cccaaattaa
    25321 aaagcaaact tacgaacaac tcagatgact atcttatgat gttatattac cacataatta
    25381 aaattaatga aattataaca aaccaaagtt tattgatttt ttaaaattta gtgacgaatt
    25441 cgcaaagaaa gttcttctaa ttgtttatca gaaacttcac caggcgcatt cgttaataaa
    25501 catgtagcag atgctgtttt agggaatgcg attgtatctc tcaagtttgt tctattagtc
    25561 aataacatga ctaatcggtc taatcctaat gcaataccgc catgtggtgg tgcaccatat
    25621 ttaaatgcat ctagtaagaa gccgaactgt tcttgtgctt gttctttagt aaatccaaga
    25681 acttcgaaca ttttttcttg taactcacca tcatgaattc tgattgaacc gccacctaat
    25741 tcataaccat ttaatactat gtcataagca tttgcctcag cttcttctgg cgcagtgcca
    25801 agcttagcaa tatcagcttc ttttggagat gtaaatggat gatgtgctgc aacgtaacgt
    25861 ttcgcatctt catcatattc taataatggc caatctgtca cccataagaa gtttaatttt
    25921 gtttcatcga ttaaacctaa ttctttagct aatttgacac gtaatgcacc taaactttgt
    25981 gcaacgacat ttggtttgtc tgcaacaaac attactaagt caccagcttc agcaccagtt
    26041 aatgtaagta atgtttcaac attttctgtt tcaaagaaac gtccaattgg acctgtcaaa
    26101 ccatcttcca caactttaac ccacgctaat cctttagcac catagatgtt tacaaattct
    26161 gttaaagcat ccatatcttt acgagtatat tgttcagccg cacctttagc gacaattgct
    26221 ttaatttcac catcattttc aacagtatct ttaaatactt taaagtccat atcacgtcct
    26281 aattgagaaa cgtcaattaa ttccatttca aaacgcgtat ctggtttatc agaaccatag
    26341 cgacgcatcg cttctttata tgtcatgcgt gggaaagcgc cattaatttc aacgccttta
    26401 acttctttaa caactttttt aagcatttct tcacccattt gcatcacatc ttcttggtct
    26461 acaaaactca tttcaatatc gacttgtgta aattcaggtt gacgatctgc acgtaaatct
    26521 tcgtcacgga agcattttac gatttggtag tatttgtcaa atccactaat catcaataat
    26581 tgcttaaata attgtggtga ttgtggtaat gcataaaatt caccatcatg aacacgagat
    26641 ggtactaaat agtcacgtgc accctcaggt gttgacttcg ttagtactgg tgtttcgatg
    26701 tcaaagaaac cttcatcatc caaatattga cgaatagaac gtgtaatttg atgtctcatt
    26761 ttaaatgttt gcgctaactc ttgacgacgt aaatctaaat aacggtattt taatcgaata
    26821 ttttcatcaa cgttaacatt ttcttcattt atagaaaatg gtggtgtctc agatttatta
    26881 atcactttaa tatttgtaac ttgtacttca acttggccag ttttaatttt aggattaact
    26941 gtttcagggt cacgcttcgt aactgtacct tgaacttcta caacatattc agaacgtact
    27001 gtttcagcaa ttttcaatgc ctcttctgaa aatgcaggat taaacacgac ttgtacaatt
    27061 ccttctctat ctcttaaatc aacgaaaatc aatccaccta ggtcacgacg attgttaacc
    27121 catcctttta atgtaatttc ttgtcctaaa aatgcttcag taactaatcc acaataagtt
    27181 gttctcttac tcatatttta acgccctctc tacttcttaa aatattcgac taatgcgtct
    27241 aattcaattg tttcagattc accagttgtc atatttttaa catcgatttt attattttct
    27301 aattcttgat caccaataac gattgtaaac ttggcaccta aacggtctgc ttgtttcatt
    27361 tgtcctttaa ttttacgctg taagtagtct ttatctgctt taataccatt atgtctcaaa
    27421 tgatttaata acttcacagc atatcgatct gcttgatcac ccattgtaac aatgaataaa
    27481 tctaagtttt cttcaatatc taattcgata ccttcttctt caagtgcaag caataatcgt
    27541 tctatactta gcgcaaaacc aatacctgtt tcacttggac catctagcaa ttctaataaa
    27601 ccattataac ggccaccacc acaaagcgtt gtaatggcac catcatagtt agggttatcc
    27661 atcattaatt caaatgctgt atgtgtataa taatccaatc cacgaactaa gttaggatct
    27721 tcaatatatg gaatacctaa atcatctaaa taagctttta cttgttcata atatgcctta
    27781 gattcctcat ttaagaaatc agtgattcta ggtgcagtct taatcgcttc tttatcacgg
    27841 tcaactttac aatccaaaat tcgcatcgga tttgtatgca aacgtgattg acaatctgaa
    27901 caaaattcat gaattactgg ttcaaagtgt ttcactaacg cttcgttata ttcttttcga
    27961 gacgccatat cccctacact attaataaca agctttaaat gttttaatcc aaatgattga
    28021 taaatatgca taaccatagc taatacttct gcatctacgc taggattttc agcaccaata
    28081 gcttctacac caaattgatt aaattgacga tagcgccctt tttgcttacg ttcatatcta
    28141 aacatcggtc cattgtaata aagtttaatt ggttggtttg gattaccttg cattttatgt
    28201 tcaatatatg aacgtacaac tgcagctgtt ccttcaggtc ttaatgtaat acttctatcg
    28261 cctttatctt taaatgtata catttctttt tgtacgacat cggttgaatc accaacacct
    28321 cttgcaaaaa gatctgtact ttcaaaaatt ggtgttctta tttctttata attataaaat
    28381 gtcattaatt catctaattg attttcaatg taacgccatt tctttgaatc ttcaggtaaa
    28441 atatcctgcg tccctctagg tattttaatc attttgacat ctccctcggc aaactatttt
    28501 atttaattac ctttttaaaa ctaaaactat atacgatcac ttaactttgc atgataaatc
    28561 ataattttaa aagcaaaaga aaaagccctt gcacagcctg aaactgcaca agggacgaat
    28621 atccgtgttg ccaccctcat tggtttaaaa aacatgccta attgtaatca tcattacttt
    28681 ttataagcgt gcattaaata aacccactca aaacgtttaa cgtacgttta acggctttac
    28741 ttttcataaa gcacctcttc ttgtgttcaa tgtatataaa ctactcagca taatctttca
    28801 gtctatggat tatattccta cacaatctta gttagattgc tacctatttc atttaccatt
    28861 tcaagaatac taacggtttt agttataaaa ttcaatatca atgataacta ctttcacata
    28921 tttttgcaag ccctacgcag aaaaataaat tttaaggcca tcaacaattg cttgttctaa
    28981 aatttgtcta tgtaattgat ctttaatcat cgtttcatca gttgggttac taatataacc
    29041 taattctaat aaaacagcag gaacttttgt ttgtcttaac acttgataat tttcttgtct
    29101 tgaaccgcga ttagaaagta gacctttctt ctgaatcgta gcgtctaacg tatctgctaa
    29161 agctctttga ttatcatgat accaataaac tgtcattcca tttgcattag atgattctaa
    29221 tgcatcatta tgtatactca aataggcatc gcctttgata tcacgatttt ctagtgaaac
    29281 atatgtatcg tctgttcttg tcatcttaac agttgcgcct tctttttcta aagtacgctg
    29341 caattctttt gctgttttca acgtatagtc tttttctaaa cttttatatt tagtattgct
    29401 tgaagcaccc tggtcactac ctccatgacc aggatcaagc actattgttt taccttgcaa
    29461 aggatttttc tccttcgtat tatccgcgac aatatctaaa tttgtgtgcc atccagctat
    29521 ccaacctttt tcattactgg atgtatcttc aacttcaatc catttaccta ctttaccaat
    29581 ctttttaaaa tggtcacctt tctcaacttt atatatgact ggatacgcag cgtttggacc
    29641 tgtacgtaat tcagcatttt cagttatcgt gatgttccca ctatcttcac tattgctatt
    29701 cagcaataaa aataaaaaga tgataaataa gacaaaggca atcactacta ttagagtacg
    29761 tttattttta agaccctttt tagataacca tgcctctatt tttttcattg aattttgccg
    29821 tcctgacttt cataaataat agtgactgga ccatcattat ttatgctaac attcatgtgt
    29881 gttccaaatt cacctgtttt cacagtaaga ccatacgctc gtagcgcatc attaaaatac
    29941 tcataaattt ttaccgcttg atcaggattt ttagaatttg agaaacctgg acggttacct
    30001 tttttacatc tgcatagaga gtaaattgtg aaactgatag tatttcacca ttcatttgtt
    30061 ggatattaaa gtttaattta ttattgtcat cttcaaataa tcttgcatta gcaattttct
    30121 ttgcaattac atctgcatct tgctctgtag agttctgacc gataccgact aataaacaat
    30181 atcctttttt gatttgatta tttaatgtat cattcgtcac cgatgcttct ttaactcttt
    30241 gtacaactac tttcatattt tgcacctcta gttccaaact cttgttactg tataaacatc
    30301 accaagttgt ttgatctttt ctaccacacg ataaacatca ttcacgtttt tcaccatgac
    30361 actaatattt attattgcat ttttatcaat atctgaacgt cctgaaactt taattaaatt
    30421 gccggctgtc gagctaacag cttgtagtac ttcattcaac aagccatttc ggtcatacgc
    30481 agttacctct aaatcaacct gatatttttg agttgcgtct tttgatttta cccattcaac
    30541 attaattagt cgttcagttt cgttcttaat atttgggcaa tcagtgcgat gtactttaat
    30601 accgtgacct ttggtgatat aacctacaat atcatcacct ggtataggat tacaacattt
    30661 tgacaacttg ataagtacat tttctaaacc ttctacatag acaccactat cagtaatgat
    30721 gttgtcttta ataggcaatg atttcgtaac ttcttgtgct tcatttaaag cacgttgttt
    30781 atctaaaata cgttgtcttt cagttaattt attaacaatc tgtagggatg tcacgccgcc
    30841 aaatcctaca gctgcgaata aatcatcttc atttgcaaag ttatattttt cattaacaac
    30901 ctgaatattt ttctctgtca aaatatcttc gactctaaat ccttgctctt ttatttcagc
    30961 ttcaaccatc attcggcctt tttcaatatt agatgaacga tcttgttttt tgaagaaact
    31021 tttaatttta cctttggcac tagacgattt aacaattttc aaccaatcac gacttggtcc
    31081 atatgaatgt ttactagtac gtatttcaac aatatcgcct gtttgtaaaa tatagtcaat
    31141 tggtacaatt ttgccattca ccttggcacc aatcatctta ttacctactt cactgtgaat
    31201 cgcataagca aaatcaatcg gcacagcacc atatggcaac tcaataacat cactcgctgg
    31261 ggtaaatgcg tatactttgt cactctgtaa gtcatatttt aaggtttcca taaattcttg
    31321 agcgtcagac gatgtatgat ccgcttcagc taattctttt aaccaattta acttattttg
    31381 ataagtttga tctttttcac ttactttttt accttctttg taagcccagt gtgctgcaac
    31441 accatgctca gcaatttcgt gcatatcaaa cgttcgtatt tggatttcga gcgggtctcc
    31501 atttggacct actactgtag tatgcaatga ctgatacaaa ttttgtttag gcattgcaat
    31561 ataatcttta aaacgtcctg gcatcggttt ccataacgta tgcaccaacc caagtatcgc
    31621 ataacaatca ttaatagaat tgacaataac acgtatcgcc aacaaatcaa aaatttgatc
    31681 aaattgtttt ttctgcttca tcattttccg ataaatactg taaatatgtt taggtctacc
    31741 atttatatcg ccttcgatat tcattcggtc catttcagta catattctat caatagccgt
    31801 ttcgatatac gcttcacgtt cactacgttt cttcttcatt aaattgacta ttctaaaata
    31861 ttgcacatta tcaatataac gaagagctgt atcttctagt tcccatttaa ttgtattaat
    31921 accaagacga tgtgctaaag gtgcataaat ttctaatgtt tctcgagaaa ttctaatttg
    31981 tttttcacgc ggcatggctt tcaaggtacg catattatgt aatctgtctg ctaatttcac
    32041 caaaattacg cgtacatctt tggcaatcgc aataaataac ttgcgatgat tttcagcttg
    32101 ttgttcttct tttgagcggt attttacttt tttaagcttc gtcacaccat caacaattcg
    32161 agcaacttct tcattgaaca tttcttttac atcttcaaat gtatacggtg tatcttcaat
    32221 tacatcatgc aaaaaacctg cgacaatcgt cggtccgtct aatcgcattt ctgttaaaat
    32281 acctgcaact tgtataggat gcataatgta tggtaatccg ttttttcgga actgaccttt
    32341 atgtgcttca taagcaatat gatagctttt taaaacatac tcatattcat ctgctgacaa
    32401 atatgatttt gctttgtgaa gaacttcatc tgcactatat ggatattcgt tgttcattat
    32461 atgatacacc ccattcatat ttattacttc gccttaaaca atgatttagg tactcttgtt
    32521 gaatagtatt tgtcccacac caatcatacg tccgtcgacg ataaatattt atcctgtcgt
    32581 cattaatcgt aatattaatt ttacttgagc gagtttaatt tgtatactat tcctactttt
    32641 aaaactttta caaaaattcg acctaaatct actgtttcat tttttaaata
//
LOCUS       DAHHOJ010000020        32500 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.020, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000020 DAHHOJ010000000
VERSION     DAHHOJ010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 32500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.020"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            320..6950
                     /gene="sasA"
                     /locus_tag="NBH32_000786"
                     /pseudo
     CDS             320..6950
                     /gene="sasA"
                     /locus_tag="NBH32_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501439.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich repeat glycoprotein adhesin SasA"
     gene            7080..8291
                     /gene="secY2"
                     /locus_tag="NBH32_000787"
     CDS             7080..8291
                     /gene="secY2"
                     /locus_tag="NBH32_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein translocase subunit
                     SecY2"
                     /protein_id="HCD1514411.1"
                     /translation="MLKLLQQYEYKIIYKRMLYTCFILFIYILGTNISIVSYNDMQVK
                     HESFFKIAISNMGGDVNTLNIFTLGLGPWLTSMIILMLISYRNMDKYMKQTSLEKHYK
                     ERILTLILSVIQSYFVIHEYVSKERVHQDNIYLTILILVTGTMLLVWLADKNSRYGIA
                     GPMPIVMVSIIKSMMHQKMEYIDASHIVIALLIILVIITLFILLFIELVEVRIPYIDL
                     MNVSATNMKSYLSWKVNPAGSITLMMSISAFVFLKSGIHFILSMFNKDVSDEMPMMTF
                     DSPIGISVYLVIQMLLGYFLSRFLINTKQKSKDFLKSGNYFSGVKPGKDTERYLNYQA
                     RRVCWFGSALVTVIIGIPLYFTLFVPHLSTEIYFSVQLIVLVYISINIAETIRTYLYF
                     DKYKPFLNQYW"
     gene            8302..9855
                     /gene="asp1"
                     /locus_tag="NBH32_000788"
     CDS             8302..9855
                     /gene="asp1"
                     /locus_tag="NBH32_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp1"
                     /protein_id="HCD1514412.1"
                     /translation="MKYFIPAWYDDQRWWQDTTVPYYQLQNKTEFDDMISLMGMHLEN
                     DLDYRLIVLNHAPNLRTFLHRYDLYETKYWSVFDEIQGFSHHAPQAINYHHLKWPDDV
                     EFVYTPYLLKCVTSEQNYTNIYFSQEGYSIWFEEFERDQLQRRYIFDDRGYLSAIRYF
                     DDHGEASYQEYLTINGDCVLYENFKNGRVTVSKRYQHHYQQTEYNNMVQLIEEKFQAM
                     IAQQIHEDDHVIVASDARHNRQIANHIPAKSLSYSFFKNRNETVSDEEYQSIVKNAHL
                     IVDSVQLERDLISHQEKYQRENTMIRITPFETRQSPNISSQLMETFIGVWIDGMSDAD
                     LQQMMQRLVDYIAQEDNYRLILLSRHQNDIPMWLRECITSVNEEYQAKQNADVNVSAL
                     MTPEDQDDIIAVKTIHAEHDVVEALRTLRLVIDMSKEPDLYLQISAISAGIPQINGQQ
                     TDYVSDYDNGRIINTVDELDDALNYYLFYLKNWNYAYAYSLKLIDAYASKNIINQLDE
                     LIEGENDAT"
     gene            9845..11413
                     /gene="asp2"
                     /locus_tag="NBH32_000789"
     CDS             9845..11413
                     /gene="asp2"
                     /locus_tag="NBH32_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp2"
                     /protein_id="HCD1514413.1"
                     /translation="MPRKFRVLQIGGDDLEPIFQHKKGVSWDYFDIGLFEFDSGYVEA
                     IEAIVEAEGRFDFIYIQAPYSETLTNLFQMISEPYNTYVDESFWSVEYEQDENVQKYV
                     VQPLHYRNIEERNNKLEAVSFSGQYGDKVSPKLALVHPNFKGDVVYQGNSELTLSGEF
                     GKEFKPIASWQNNLVYDKDKVIQIWPEFDIDGAVELQYTFRLIQTGADGALIEQIILT
                     DDMLDSPLEIPTKPFDAYISVTVKARGNGTVHLGPIHKRWSRLDMGQFLLGGSRFVDS
                     QRQEFIYYFHPGDMKPPLNVYFSGYRTAEGFEGYYMMKRMNAPFLLIGDPRVEGGSFY
                     IGSSEYEQGIINVIDETLEKLNFKSHELILSGLSMGSFGALYYGAQLNPQAIIVGKPL
                     VNIGTIAEHMRLLRPEEFGTALDVLVSNEGDTSQASIQALNQKFWQTFQKKSLSQTVF
                     AIAYMQHDDYDPHAFQELLPVLTAHQARVMNCSIPGRHNDDSPTIASWFVNFYNIILE
                     DKFGRVQHAEKQNI"
     gene            11391..12350
                     /gene="asp3"
                     /locus_tag="NBH32_000790"
     CDS             11391..12350
                     /gene="asp3"
                     /locus_tag="NBH32_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system protein Asp3"
                     /protein_id="HCD1514414.1"
                     /translation="MLKNKTFKVTWNYISQTTFMYGSKVSFSNGEVTFINPLMPSGLP
                     IHEWLMLKQFSKYKSAPSLPILRRGQHYKLHFDFDATPAGSVYFIIIFYNKNGTKLST
                     EIVKSNSITIQYPDEAYAYKIKMMNAASTSLIFRCLTITEMTHQDDLEYKSMRVTKIG
                     DDQYGNDMINVIIAEPSDTYPTISNDFLKLFGHVWLVERWMDDNIEGNIKQLKNDLQS
                     QDTLKAINLISYGSKSNVFATYVAQHLDCKVYRTSHEDDDLKEWLTEHVPGNHELKDT
                     NVEVYFKEEQDNHLNYMSRLMNPVRFLDYLDVSKLNGGEVNET"
     gene            12340..14730
                     /gene="secA2"
                     /locus_tag="NBH32_000791"
     CDS             12340..14730
                     /gene="secA2"
                     /locus_tag="NBH32_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system translocase SecA2"
                     /protein_id="HCD1514415.1"
                     /translation="MKHKLDVTINELRLKSIRKIVKRINTWSDEVKSYSDDALKQKTI
                     EFKERLASGVDTRDTLLPEAYAVAREASWRVLGMYPKEVQLIGAIVLHEGNIAEMQTG
                     EGKTLTATMPLYLNALSGKGTYLITTNDYLAKRDFEEMQPLYEWLGLTASLGFVDIVD
                     YEYQKGEKRNIYEHDIIYTTNGRLGFDYLIDNLADSAEGKFLPQLNYGIIDEVDSIIL
                     DAAQTPLVISGAPRLQSNLFHIVKEFVDTLIEDVHFKMKKTKKEIWLLNQGIEAAQSY
                     FNVEDLYSEQAMVLVRNINLALRAQYLFESNVDYFVYNGDIVLIDRITGRMLPGTKLQ
                     AGLHQAIEAKEGMEVSTDKSVMATITFQNLFKLFESFSGMTATGKLGESEFFDLYSKI
                     VVQVPTDKAIQRIDEPDKVFRSVDEKNIAMIHDIVELHETGRPVLLITRTAEAAEYFS
                     KVLFQMDIPNNLLIAQNVAKEAQMIAEAGQIGSMTVATSMAGRGTDIKLGEGVEALGG
                     LAVIIHEHMENSRVDRQLRGRSGRQGDPGSSCIYISLDDYLVKRWSDSNLAENNQLYS
                     LDAQRLSQSSLFNRKVKQIVVKAQRISEEQGVKAREMANEFEKSISIQRDLVYEERNR
                     VLEIDDAENRDFKALAKDVFEMFVNEEKVLTKSRVVEYIYQNLSFQFNKDVACVNFKD
                     KQAVVTFLLEQFEKQLALNRKNMQSAYYYNIFVQKVFLKAIDSCWLEQVDYLQQLKAS
                     VNQRQNGQRNAIFEYHRVALDSFEVMTRNIKKRMVKNICQSMITFDKEGMPVIHFP"
     gene            14748..16256
                     /gene="gtfA"
                     /locus_tag="NBH32_000792"
     CDS             14748..16256
                     /gene="gtfA"
                     /locus_tag="NBH32_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosyltransferase GtfA"
                     /protein_id="HCD1514416.1"
                     /translation="MTIYNINFGIGWASSGVEYAQAYRAKLLRRTNQDTKFVFLDFIQ
                     SENIQTLTQNIGFKDQEVIWLYQYFSDISIAPTTYTLDDVERELGHDISFRERNGKIV
                     RLYFNGKSSFVTCYLQNEQKDVVDRAEFVINSMLVRKDFYSYTRIFSEYYAPADNKAK
                     LYMRQFYNEDGSIAYNEYIDGDSSIYVFEDAVLYNKTEFIAYFLQRLNLTRDDIVILD
                     RASDIGQAVLQHKGDSKVGVVIHADHYSNNMMSEQHILWNNYYEYQFSKAKYIDFFIT
                     ATDIQNHMVCRQFEQYQGYRPRVYTIPVGSIDALSYPTLSRKPYAMISASRLANEKHI
                     DWLVKAVIVAKRQVPELTFDIYGEGSEKTRLRKIIDTHRAQDYIRLLGHVKLDEIYND
                     YELFLSASTSEGFGLTLMEAVGSGLGMIGLNVNYGNPTFIRDGENGYLVPFDTDEDSV
                     DDVVAKLAHAIVMYFNNGPQTPHDISYEVAQQFMTQDIILKWETLVQEVLHD"
     gene            16249..17607
                     /gene="gtfB"
                     /locus_tag="NBH32_000793"
     CDS             16249..17607
                     /gene="gtfB"
                     /locus_tag="NBH32_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory Sec system glycosylation chaperone
                     GtfB"
                     /protein_id="HCD1514417.1"
                     /translation="MIKLFDYFNDHSRKLYESFKASKLEEDLTIVLNDNGFLPDDVIS
                     PYQFFADNHNTENMKPRFFNQVTVPAFWEIKGNNNSATINDMGRLRGKIFYQSGERPR
                     IVSRVEWFDDQQRVRFVDYYSKNGIKFAQTVYDLNRKAILKKYMTAEGKEVIYENFVT
                     SDVILDWQGKSYFFPSKLAFVLFFIKQLEITEHHFVINSLALPFSVLYNLPSNGSDVL
                     VWQEQCDGNVPGNMQLMCKGDMKRHCNIIIPDKNEYETMLNIADAKVQSRILQGGYLY
                     NYRSRNRYTKEIVILTNSDQLRNIKVLVETLPDFKFHIAAITEMSDKLMQLDQYANVH
                     LYPSINIDRVNELYQLCDIYLDINEGNEILNAVEQAFDYELLILGYRQTAHHAKVTLS
                     EHLFEHNDEITMESKDQLIQMLESLKDQQQFRDALLAQKAHAHEISREQFEQVFKQAL
                     ES"
     gene            17849..19756
                     /gene="sasF"
                     /locus_tag="NBH32_000794"
     CDS             17849..19756
                     /gene="sasF"
                     /locus_tag="NBH32_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-wall-anchored protein SasF"
                     /protein_id="HCD1514418.1"
                     /translation="MAKYRGKPFQLYVKLSCSTMMATSIILTNILPYDAQAASEKDTE
                     ISKEILSKQDLLDKVDKANRQIEQLKQLSASSKAHYKAQLNEAKTASQIDEIIKRANE
                     LDSKDNKGSQIEMNGQSDIDSKLDQLLKDLNKVSSKVDRGQQSGKDDLNAMKNDMSQT
                     ATTKYGEKDNENDEAMVDKALEDLDHLNQQIHKSKDASKDTSEDPAVSTTDNNHEVAK
                     TPNNDGSGHVVLNKFLSNEENQSHSNRLTDKLQGSDKINHAMIEKLAKSNASTQHYTY
                     HKLNTLQSLDQRIANTQLPKNQKSDLMSEVNKTKERIKSQRNIILEELARTDDKKYAT
                     QSILESIFNKDEADKILKDIRVDGKTDQQIADQITRHIDQLSLTTSDDLLTSLIDQSQ
                     DKSLLISQILQTKLGKAEADKLAKDWTNKGLSNRQIVNQLKKHFASTGDTSSDDILKA
                     ILNNAKDKKQAIETILATRIERQKAKLLADLITKIETDQNKIFNLVKSALNGKADDLL
                     NLQKRLNQTKKDIDYVLSPIVNRPSLLDRLNKNGKTTDLNKLANLMNQGSNLLDSIPD
                     IPTPKPEKTLTLGKGNGLLSGLLNADGNVSLPKAGETIKEHWLPISVIVGAMGVLMIW
                     LSRRNKLKNKA"
     gene            19923..20483
                     /locus_tag="NBH32_000795"
     CDS             19923..20483
                     /locus_tag="NBH32_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1514419.1"
                     /translation="MSRKTALLVLDMQEGIASSVPRIKNIIKANQRAIEAARQHRIPV
                     IFIRLVLDKHFNDVSSSNKVFSTIKAQGYAITEADASTRILEDLAPLEDEPIISKRRF
                     SAFTGSYLEVYLRANDINHLVLTGVSTSGAVLSTALESVDKDYYITVLEDAVGDRSDD
                     KHDFIIEQILSRSCDIESVESWKSSL"
     gene            complement(20750..22609)
                     /locus_tag="NBH32_000796"
     CDS             complement(20750..22609)
                     /locus_tag="NBH32_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase domain-containing protein"
                     /protein_id="HCD1514420.1"
                     /translation="MPKNKILIYLLSTTLVLPTLVSPTAYADTPQKDTTAKTTSHDSK
                     KSNDDETSKDTTSKDTDKADNNNTSSQDNNDKKFKTIDDSTSGSNNIIDFIYKNLPQT
                     NINQLLTKNKYDDNYSLTTLIQNLFNLNSDISDYEQPRNGEKSTNDSNKNSDNSIKND
                     TDTQSSKQDKADNQKAPKSNNTKPSTSNKQPNSPKPTQPNQSNSQPASDDRANQKSSS
                     KDNQSMSDSALDSILDQYSEDAKKTQKDYASQSKKDKNEKSNTKNPQLPTQDELKHKS
                     KPAQSFNNDINQKDTRATSLFETDPSISNNDDSGQFNVVDSKDTRQFVKSIAKDAHRI
                     GQDNDIYASVMIAQAILESDSGRSALAKSPNHNLFGIKGAFEGNSVPFNTLEADGNQL
                     YSINAGFRKYPSTKESLKDYSDLIKNGIDGNRTIYKPTWKSEADSYKDATSHLSKTYA
                     TDPNYAKKLNSIIKHYQLTQFDDERMPDLDKYERSIKDYDDSSDEFKPFREVSDSMPY
                     PHGQCTWYVYNRMKQFGTSISGDLGDAHNWNNRAQYRDYQVSHTPKRHAAVVFEAGQF
                     GADQHYGHVAFVEKVNSDGSIVISESNVKGLGIISHRTINAAAAEELSYITGK"
     gene            22820..25801
                     /locus_tag="NBH32_000797"
     CDS             22820..25801
                     /locus_tag="NBH32_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HCD1514421.1"
                     /translation="MKNAFKLFKMDLKKVAKTPAVWIILAGLAILPSFYAWFNLWAMW
                     DPYGNTGHIKVAVVNEDKGDTIRGKKVNVGNTMVNTLKKNKSFDWQFVSREKADHEIK
                     MGKYFAGIYIPSKFTHEITGTLRKQPQKADVEFKVNQKINAVASKLTDTGSSVVVEKA
                     NEQFNKTVTRALLEEANKAGLTIEENVPTINKIKNAVYSADKALPKINDFANKIVYLN
                     NHQADLDKYANDFRKLGNYKGDILDAQKKLNEVNGAIPQLNEKAKLILALNNYMPKIE
                     KALNFAADDVPAQFPKINQGLNIASQGIDQANGQLNDAKDFVTQVRSRVGDYQDAIRR
                     AQDLNRRNQQQIPQNSAANNETSNSAPAAGNGVASTPPSVPSGDTAPNNNVTQNTAPN
                     GNNAPVSTTPQSTSGKKDGQSFADITTTQVSTANENTQNITDKDVKSMEAALTGSLLS
                     LSNNLDTQAKAAQKDSQALRNISYGILASDKPSDFRESLDNVKSGLEYTTQYNQQFID
                     TLKEIEKNENVDLSKEIDKVKAANNRINESLRLVNQLSNALKNGSSGTAEATKLLDQL
                     SKLDSSLSSFRDYVKKDLNSSLVSVSQRIMDELNKGQTALSNVQSKLNTIDQVINSGQ
                     AILKNGKTRIDRLQTVLPSIEQQYISAVKNAQANFPKVKSDVAKAANFVRNDLPQLEQ
                     RLTNATASVNKNLPTLLNGYDQAVGLLNKNQPQAKKALSDLADFSQNKLPDVEKDLKK
                     ANKIFKKLDKDDAVDKLIDTLKNDLKKQAGIIANPINKKTVDVFPVKDYGSGMTPFYT
                     ALSVWVGALLMVSLLTVDNKHKSLEPVLTTRQVFLGKAGFFIMLGMLQALIVSVGDLL
                     ILKAGVESPVLFVLITIFCSIIFNSIVYTCVSLLGNPGKAIAIVLLVLQIAGGGGTFP
                     IQTTPQFFQNISPYLPFTYAIDSLRETVGGIVPEILITKLIILTLFGIGFFVVGLILK
                     PVTDPLMKRVSEKVDQSNVTE"
     gene            complement(25913..26851)
                     /gene="manA"
                     /locus_tag="NBH32_000798"
     CDS             complement(25913..26851)
                     /gene="manA"
                     /locus_tag="NBH32_000798"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HCD1514422.1"
                     /translation="MALFLQPVFKDRIWGGHALKAFNYDIPNETTGECWAISAHPNGP
                     NTIINGPYKDMTLDQLWSQHRELFDNDSRDSFPLLTKVLDANDKLSVQVHPDDDYALK
                     HEGELGKTECWYILDAAPGAEIIYGVHAHNKQTLIDMIDNHEFDSLFKRIPVKPGDFY
                     YVPAGTVHAIGSGILILETQQSSDTTYRIYDYDRRDQNGQLRDLHLEQSKDVIELGNH
                     EPNTTPITTHIDTHTVTQFVDNQYFAVYKWDIHGDLKFNKPHAYCLVTIIDGTGTLTV
                     NDKHYDIPKGSSFILTTEDEDIHFVGNISAIVSHPG"
     gene            complement(26865..28817)
                     /locus_tag="NBH32_000799"
     CDS             complement(26865..28817)
                     /locus_tag="NBH32_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIABC"
                     /protein_id="HCD1514423.1"
                     /translation="MKIVAITSCPNGIAHTYMAQEKLEQVAKEMGVDIKVETQGGVGA
                     ENVLTTQDIEEADGVIIAADKQVDLSRFVGKRLINENVREGIHNPRGLIQRIINQDAP
                     IYQSETNYHSKDRGKSKSGIQMVYQHLMNGVSFMVPFIVVGGLLIAIALTLGGETTSK
                     GLVIPDDSFWKSIENIGSLAFKFMVPILAGYIAVSIADKPGLVPGMIGGAIAADGSFY
                     GSDAGAGFLGGIVAGFLAGYIAKWIKDIKVPKAMAPIMPIIIIPIISSVVVGLIFIFL
                     IGAPISNIFEALTTWLKSMQGANIIILAMIIGAMIAFDMGGPVNKVAFLFGSALIAEG
                     NYAVMGMVAVAVCTPPIGLGLATFVQKYKFNHSEREMGKASFTMGLFGITEGAIPFAA
                     QDPLRIIPANIIGAMIASVIAAIGGVGDRVAHGGPIVAVLGGIDHVLLFIFAVIVGSL
                     VTMTTVLLLKRNTPVIAVDAPAQHTQLHDTDITQHDTEVDNVDNTAKHSHHNKPSHVF
                     DQQTMIITDHDMSRNEAIDMLIHKLKICRYVEHTSHLKNAILEREMESTTAIGMNVAI
                     PHAKSDVVKQPIVAVMKNNHGVKWESLDGSLPQLIFLIAVPNNSQDTHLKILQRLSKA
                     LMNDETRQSLINANSTTEIYNLLMKI"
     gene            complement(28894..30768)
                     /locus_tag="NBH32_000800"
     CDS             complement(28894..30768)
                     /locus_tag="NBH32_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HCD1514424.1"
                     /translation="MLDRHLKLLQFFIKNPSKHISSNEIAEHVNVSNRTVRNDIHVIN
                     SNFMDDIIVSIKSKGYQLNTSQYTLETITERYTHIQSYKEKLLLSMAYQLLMHNKSQT
                     LQQLEQDYLLSKTVLNDYFIRIQQWCQKFNIALTIKKKQGIVVDGTDNDITNAIIHLN
                     QLSSGHVHVEDLILNELPDSHQRMISHIIQETLQQHSIETTDIQIQQLLIHLILIIKR
                     KQSLSELDTLNHDAKLISQSCIKQINERLGYDLNEQVINMFSFFIAYHFNKLDIGIER
                     IFIQSYIDRLIELMQQRIHVPFNEDSILQQNLYNHFSKAYLRITQNIYLNNPLVIEIK
                     KLYPFVFNTLFEAIDKLAVDTDIEMSEDEIAFLTIHFQAAIERRTKTQLNVVIACYYG
                     LGVSNFLETKINNLSEELLVINTIKLENITHYHFDNVDLLITTHDIPKQTLNILPKHL
                     STIKVAPLFSEDDRHKIRHIVKQKQNPVQAHHHMDTVNFLVVNTEQKPRHTVQIFEEA
                     QKILQAHHAIVEGYIESALEREKSSSTYIGNFMAIPHGDPEKVLQSHVLIFRTKDVFP
                     WRQHDVKLVFFLAISQKDTAFTKQMMQLIANLDDDSVNHLCNLDDHSLKQQLFEYLQE
                     "
     gene            complement(31008..31481)
                     /locus_tag="NBH32_000801"
     CDS             complement(31008..31481)
                     /locus_tag="NBH32_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000714021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514425.1"
                     /translation="MKKFLITVVIILLIGALGFAIYAAINHSTSSNHNNEQDNKATHQ
                     KKDTEVNHQKDENTNNTNENQNNNGVTNGNQSSTPSTVQNNGSTAPANTQPSNPNQSN
                     SNQSNPNQSNSNHGGSSQPSTNNQNHKQQNTPPANKSNNTNTPSKQTPQVQSPKN"
     gene            31736..32263
                     /gene="isaB"
                     /locus_tag="NBH32_000802"
     CDS             31736..32263
                     /gene="isaB"
                     /locus_tag="NBH32_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="immunodominant staphylococcal antigen IsaB"
                     /protein_id="HCD1514426.1"
                     /translation="MNQTTKLCAATTLALGTLIGATVVGTASPIHQETQAATTPYYTY
                     HGYIGHNANFIMDKHFINAIKYDNVTFNGIKLAKTNATKKVEKYDQTFKGVTAKGNEA
                     NQLQFIVKNNISLKDIQKAYGKDLKKENNKTKESDSGIFYYQHSKKSLGIWFVVDHNR
                     VVEVTVGHTPYKTSK"
BASE COUNT        11304 a         5100 c         6059 g        10037 t
ORIGIN      
        1 ctattaatga ataaaaggtg gatgataaat acattttaat aaaaagaagt ttttaccaaa
       61 taaataaaag atgtccaaga tgataagcat tgatttcaaa attatataaa cataataagg
      121 catattattc agaatatagt taaaaaatta tactaaatcc cacacaataa agaaagcgat
      181 ttcatcaaag aatatacttt tggaaaaatt ggattttgac aaattaagta ttttttattt
      241 gaaaaaagag ttgtattaaa ttgaatagtg gcatatatta taatctaatg aatgtaaaga
      301 taatacaagg agttattaca tgagcaaaag acagaaagaa tttcatgaca gcttagcaaa
      361 cgaaaaaaca agagtaagac tttataaatc tggaaaaaat tgggtaaaat ccggtattaa
      421 agaaatagaa atgttcaaaa taatgggact accatttatt agacatagta tagtgagtca
      481 agataatcaa agcattagta aaaaaatgac tggatacgga ctgaaaacga cggcggttat
      541 tggtggtgca ttcacggtaa acatgttgca tgaccagcaa gcttttgcgg cttctgatgc
      601 accattaaca tctgaattaa atacacaaag tgaaacagta ggtaatcaaa actcaacgac
      661 aatcgaagca tcaacatcaa cagccgattc cacaagcgta acgaaaaata gtagttcggt
      721 acaaacatca aatagtgaca cagtctcaag tgaaaagtct gaaaatgtca cttcgacaac
      781 taatagtaca agtaatcaac aagaaaaatt aacatctaca tcagaatcaa catcctcaaa
      841 gaatactaca tcaagttctg atactaaatc tgtagcttca acttcaagta cagaacaaca
      901 aactaatata tcaacaaatc aaagcactgc atcaaatact acttcacaaa gcacagcgcc
      961 agcttcaacc catttaaaac aaactagcac aacgtcaacc agcacagcac ctgtcaaact
     1021 tcgaactttc agtcgattag ctatgtcaac ctttgcatca gctgcgacga caaccgcatt
     1081 aactgctaat acaattacag ttaataaaga taacttaaaa caatatatga caacttcagg
     1141 taatgccact tatgaccaaa gtacaggggt cgtaacgtta acgcaggatg catatagcca
     1201 aaaaggtgct attacattag gaacacgtat tgactctaat aagagttttc atttttctgg
     1261 aaaagtaaat ttaggtaata aatacgaagg taatggaaat ggtggagatg gtatcggctt
     1321 tgccttttcg ccaggtgtat taggtgaaac agggttaaac ggtgccgcag taggtattgg
     1381 tggcctaagt aacgcatttg gcttcaaatt ggatacgtat cacaatacat ctaaaccaaa
     1441 ttcagctgca aaggcgaatg ctgacccatc tagtgttgct ggtggaggtg cgtttggtgc
     1501 ctttgttaca acagatagta ctggcgttgc gacaacgtac acatcaagtt caacagctga
     1561 taatgctgca aagttaaaag ttcaacctac aaacaacacg ttccaaaatt ttgatattac
     1621 ttataatggt gatacaaagg ttatgactgt cacgtatgca ggtcaaacat ggacacgtaa
     1681 tatttcagat tggattgcaa aaagtggtac gaccaacttt tcattatcaa tgacagcctc
     1741 aacaggtggc gcgacaaatt tacaacaagt acaatttgga acatttgaat atacagagtc
     1801 tgctgttaca caagtgagat acgttgatgt aacaacaggt aaagatatta ttccaccaaa
     1861 aacatattca ggaaatgttg atcaagtcgt gacaatcgat aatcaacaat ctgcattgac
     1921 tgctaaagga tataattaca catctgtcga tagttcatat gcatcaactt ataatgatac
     1981 aaataaaact gtaaaaatga cgaatgctgg acaatcagtg acatattact ttactgatgt
     2041 aaaagcacca actgtaactg taggcaatca aaccattgaa gtgggtaaaa caatgaatcc
     2101 tattgtgttg actacaacgg ataatggtac agggactgtg acaaatacag ttacaggatt
     2161 accaagcgga ttaagttatg atagtgcaac gaattcaatc attgggacac caacaaaaat
     2221 cggtcaatca acagtgacag tagtatctac tgaccaagca aataacaaat cgacgacaac
     2281 ttttacaata aatgttgtgg atacgacagc accaacagtg acgccaatag gagataaatc
     2341 atcagaagtt ttttctccaa tatcaccgat taaaattgct acgcaggaca atagtggtaa
     2401 tgcagtaaca aatacagtca caggattgcc gtctggacta acattcgata gtacaaataa
     2461 taccattagt ggtacaccaa caaacattgg tacaagtact ataacaatcg tttctacaga
     2521 tgcgagcggt aacaaaacga cgacaacttt taaatatgaa gtaacaagaa atagtatgag
     2581 tgattccgta tcaacatcgg gaagtaccca acaatcacaa agtgtgtcaa caagtaaagc
     2641 tgactcacaa agtgcatcaa cgagtacatc aggatcgatt gtggtatcta catcagctag
     2701 tacctcgaaa tcgacaagtg taagcctatc tgattctgtg agtgcatcta agtcattaag
     2761 cacatctgaa agtaatagtg tatcaagctc aacaagcaca agtttagtga attcacaaag
     2821 tgtatcatca agcatgtcag gttcagttag taaatcaaca tcattaagcg attctatttc
     2881 gaactctagc agtactgaaa aatccgaaag tctatcaaca agtacatctg attcattgcg
     2941 tacatcaaca tctttaagtg attcagtaag catgagtaca tcaggaagct tgtttaagtc
     3001 acaaagctta tcaacaagta catccgattc ggctagtact tcacaatcgg taagtgatag
     3061 tacatcaaat tcaattagta cgtcagaatc attgagtgag tcagttagta catcagaatc
     3121 tataagtatt tctaatagca tttcgaattc agtgagtgca tcaacaagca aatcggaatc
     3181 acaaagtaca tcaatatcat taagtacaag cgattctaaa tcgatgagta catcagaatc
     3241 attaagcgat tcaacgagca caagtgattc ggtatctgga tcattaagtg tagcagggtc
     3301 acaaagtgtc tcaacaagca catcagactc aatgagcacg tctgaaatga taagcgattc
     3361 tatgagtaca agtggatcat tagctgcatc agacagtaaa tcaatgtctg taagtagttc
     3421 gatgagcact tctcagtcag ttagtacatc agaatcatta agtgattcga taagtacatc
     3481 ggattctgac agtaagtcat tatcattaag tactagtcaa tcaggttcaa caagtacatc
     3541 aacgtcgaca agtgcttcag tacgtatgtc ggaatcacaa agtatgtctg gttcaatgag
     3601 tgcaagtcaa tccgattcaa cgagcatatc aacgtcgttt agtgattcaa caagtgatag
     3661 caaatcagca tcaacggcct caagtgaatc agtatcacaa agtgtctcaa caagtacatc
     3721 tggttcggtg agtacttcga catcgctaag tactagtagt tcagaacgca catcaacatc
     3781 attgagtgct tcgacaagtt taagcacatc agagtctggt tcaacgagcg actcaacgtc
     3841 gacgagcgat tctatcagtg aagcgatatc tggttcagag agtacgtcta tatcattaag
     3901 tgaatcaacg agtaccagtg attcagaatc acaaagtgca tctgcttttt taagtgaatc
     3961 attgagtgaa agcacatccg aatccaacat cagagtcatt aagtggctca acaagcgata
     4021 gtatgtcttt atcggatagt aattctgaat ctggtagcac atcaacatca ttaagtaatt
     4081 caacaagtgg tagtacgtcc atttcaacat caacaagtaa tagtgcatca acgtcaacgt
     4141 ttaagagcga gagtgtctca acatcattga gcacatcaac gagtacaagt gtgtcaaatt
     4201 caacaagcgc atcgacatca ttaagtgatt cagcaagtgg tagcaagtcc aattcactaa
     4261 gtgcatcaat gtcgacaagt gattcaatca gtacaagaaa atcagagtca ttaagtgcat
     4321 cgacatcatt aagcggttca acgagtgaga gtgaatccgg ctcaacatca tcaagtgaaa
     4381 gtaaatctga ttcaacatca atgagcctga gcatgtccca atcaacatca ggaagtacaa
     4441 gtgtgtcaac atcagaaagt ttgtctgact caacgagtac atcattgtca ctaagtgcct
     4501 caatgaatca aagcggtgta gactcaaact cagcaagtca aagtgtctca aactcaacaa
     4561 gtacaagcac gagcgaatcc gattcacaaa gcacatcatc atatacaagt cagtcaacaa
     4621 gccaaagtga atccacatcg acatcaacgt cactaagcga ttcaacaagt atatctaaaa
     4681 gtacgagtca atcaggttcg gtaagcacat cagcgtcatt aagtggttca gagagtgaat
     4741 ctgattcaca aagtatctca acaagtgcaa gtgaatcaac atcagaaagt gcgtcaacat
     4801 tactcagtga ctcaacaagt acaagtaact caggatcagc aagtacgtca acatcgctca
     4861 gtaactcagc aagcgcaagt gaatccgatt caacatcaac atcattaagc gattcacaaa
     4921 gcacatcaag aagtacaagt gcatctggat cggcaagtac atcaacatca acaagtgact
     4981 ctcgtagtac atcagcttca actagtactt cgatgcgtac aagtacgctt gattcacaaa
     5041 gtatgtcgct ttcaacaagt acatcaacaa gtgtgagtga ctcaacgtca atatctgata
     5101 gtgttagtga ttcaacatca gactcaacaa gtatgagtat atccgattca atgagtgtat
     5161 ctgtatcatt aagtgattcg acaagcacat caacatcggc tagtgaagta atgagcgcaa
     5221 gcatatcgga ttcacaaagt atgtcagaat ctgtaaatga ttcagaaagt gtaagtgaat
     5281 ctaattctga aagtgactct aaatcgatga gtggctcaac aagtgtcagt gattctggct
     5341 cattgagcgt ctcaacgtca ttaagaaaat cagaaagtgt aagcgagtca agttcattga
     5401 gtggctcaca atcgatgagc gattcagtaa gcacaagcga ttcgtcatca ttaagtgtat
     5461 cgacgtcact aagaagttcg gaaagcgtga gtgaatctga ttcattaagt gattcaaaat
     5521 cgacaagtgg ttcgacttca acaagtacat ctggttcatt gagtacatca acatcattaa
     5581 gtggttcaga aagcgtaagc gagtctacct cgctaagtga ttcaatatca atgagtgatt
     5641 ctactagtac aagtgactcc gactcattaa gtggatcaat atctttaagt ggttccacaa
     5701 gtcttagcac ttcggattca ttaagtgatt caaaatcatt gagtagctcg caaagtatga
     5761 gtggatcaga atcaacgtca acaagtgtga gcgattcgca gtcaagctca acaagtaata
     5821 gtcaatttga ctctatgagc atcagtgcat cagaaagcga ctcaatgtct acaagtgatt
     5881 cgtctagcat cagtggatca aattcaacga gtacatcact ttcaacatct gactcaatga
     5941 gcggaagcgt atcagtttca acatcgacaa gtttaagtga ctcaatatca ggttcaacaa
     6001 gtttaagtga ctcgagctca acaagcacat ctacatcatt aagtgattca atgtcacaaa
     6061 gccagtcaac aagtacaagt gcatctggtt ccttaagtac atcgatatca acatcaatgt
     6121 caatgagtgc tagtacatcg tcatcacaaa gcacatcggt gtcgacatca ttatcaacat
     6181 cagacagtat cagtgattct acttcaataa gtatcagtgg ttcacaaagt acagtagaat
     6241 cagaatctac aagtgattca acttctatca gtgactcaga atcattgagt acatcagatt
     6301 cagactcgac atcgacaagt acatcggact caacaagtgg ttcaacttca acaagcatat
     6361 ctgaatcatt aagtacgtct ggttcaggtt caacgagcgt atctgactca acatcaatga
     6421 gtgaatccga ttcaacaagt gtttcaatgt cacaaagtat gtccggttca acgtacaact
     6481 cgacttcagt gagcgactca gaatcagtgt caacaagcac atcaacgtca ttgagcacat
     6541 ccgattcgac aagcacatcc gaatcgctga gtacatctat gtctggttca caaagcattt
     6601 ctgactcaac atcaacaagt atgtccggct caacaagtac atctgaatct aactcaatgc
     6661 atccgtcaga ctcaatgagt atgcatcata ctcacagcac gagcacatct cgcttatcaa
     6721 gtgaagcaac aacgagcacg agtgaatctc agtctacatt aagtgcaaca tctgaagtga
     6781 ctaaacataa tggcacgcct gcacaaagtg aaaaaagatt gccagataca ggtgactcaa
     6841 taaaacaaaa tggattacta ggtggcgtta tgacattatt agttggttta ggtttaatga
     6901 agagaaagaa aaagaaagat gaaaatgatc aagatgattc tcaagcataa attgaattta
     6961 tagctggcta aaattaaaaa gactgaggca ccaatcacta gaaaaatcga tataggattt
     7021 taataattag tgatgaaccc ttagtctttt taatgttttt gatataaaag aggtgcaaca
     7081 tgttaaaact tttacaacaa tacgaatata aaattatata taaacgtatg ctatacacat
     7141 gttttatatt atttatttat atattaggta caaacatttc catcgtttcc tataacgata
     7201 tgcaagtaaa acatgagtca ttttttaaaa tagctatttc taatatgggt ggagacgtta
     7261 atactttaaa cattttcact ttaggattag gtccgtggtt aacatcaatg attatcttga
     7321 tgcttatttc ttatcggaat atggataaat acatgaaaca aacaagtctt gagaagcatt
     7381 ataaagagcg catcttaaca ttaattttaa gtgttattca aagttatttt gtgattcatg
     7441 aatatgtttc gaaagaacgt gttcatcaag acaatattta cttaacaata ttgattttgg
     7501 ttactggaac gatgttatta gtttggttag ctgacaaaaa tagtcgttac ggcatcgcag
     7561 gaccaatgcc gattgtaatg gttagtatta ttaaatcaat gatgcatcaa aaaatggaat
     7621 atatagatgc aagtcatatt gtgattgcgt tgttaatcat ccttgtgata atcacattgt
     7681 ttatcttatt atttattgaa ttagtagaag tgagaatacc atatatcgat ttaatgaacg
     7741 tttccgcaac aaatatgaaa tcttatttat cttggaaagt taaccctgca ggcagtatta
     7801 ctttaatgat gagcatttca gcttttgttt tcttaaaaag tggcattcat tttattttat
     7861 ctatgtttaa taaagacgtt tcagatgaaa tgccaatgat gacattcgat agtccaattg
     7921 gtatttcagt atatttagtg attcaaatgt tattgggtta ttttttatcg agatttttaa
     7981 taaatacaaa acaaaaatca aaagacttct taaagagtgg caattatttt tcaggagtta
     8041 aacctggtaa ggatacagaa cgttatttaa attatcaagc acgacgcgta tgttggtttg
     8101 gatcggcatt agttacagtc attattggta taccacttta tttcacattg tttgtaccgc
     8161 atttatctac tgaaatttat ttctcagtac aactgattgt attagtttat atcagtatta
     8221 atattgcaga aacaattcgt acatatttat attttgataa atataagcca tttttaaacc
     8281 agtattggta aggaggtaat tatgaaatac tttattccag cttggtacga tgaccaacga
     8341 tggtggcaag acacgactgt gccgtattat caactacaaa ataagacgga atttgacgat
     8401 atgattagtt taatgggaat gcaccttgaa aatgacttag attatcgtct gattgttctc
     8461 aatcatgcac caaatttaag aacattttta catcgatatg acttatatga gacaaagtat
     8521 tggtctgtgt ttgatgaaat tcaaggattc agtcaccatg cgccacaagc gattaattat
     8581 caccacttaa aatggccgga tgacgtagaa ttcgtgtaca cgccgtattt attaaaatgt
     8641 gtgacgagtg aacagaacta tacaaatatt tattttagtc aagaagggta ttcaatttgg
     8701 tttgaagaat ttgaaagaga tcagttacaa cggcgttata tttttgatga cagaggttat
     8761 ttatcagcga tacgttattt tgatgatcat ggagaggctt cttaccaaga atatttaacg
     8821 attaatggag attgtgtgct ttacgaaaac tttaaaaatg gacgagtcac tgtttcaaaa
     8881 agatatcaac atcattatca acaaacagaa tataacaata tggttcaact aattgaagaa
     8941 aaatttcaag caatgattgc acaacaaata catgaagatg atcatgtgat tgtggcttca
     9001 gatgctaggc acaatcgaca aatagccaat catattccag cgaaatcatt aagttattca
     9061 ttttttaaaa atagaaatga aactgtgtca gatgaggaat atcaatctat cgtaaagaat
     9121 gcccatttaa ttgttgatag tgtgcaacta gaacgtgatt taattagtca tcaagagaag
     9181 tatcagcgag agaatacaat gattcgaatc acaccatttg aaacgagaca atcacctaat
     9241 ataagtagtc aattgatgga aacatttata ggtgtatgga tagatggtat gagtgacgct
     9301 gatttgcaac aaatgatgca acgacttgtg gattatatcg cacaagaaga taattaccgt
     9361 ttaattttat tatcacgtca tcaaaatgac ataccaatgt ggcttcgtga atgtattacg
     9421 tcggtaaatg aggaatacca agctaaacag aatgcggatg ttaatgtttc agcattaatg
     9481 acacctgaag atcaagatga cattattgct gttaagacga tacatgctga acatgatgtt
     9541 gtagaagcat tgcggacgtt gcgacttgtg atagatatgt caaaagaacc tgacttgtat
     9601 ttacaaatta gtgcaattag cgctgggatt ccacaaatta atggtcaaca aacagattac
     9661 gtctctgatt atgacaatgg ccgtattata aatacagttg atgaattaga tgatgcgtta
     9721 aattattatt tattttattt gaaaaattgg aattatgcgt acgcctattc tttaaaatta
     9781 atagatgcat atgcttctaa gaatattatt aatcagctcg atgagttaat agaaggtgaa
     9841 aatgatgcca cgtaaattta gagttttgca aattggagga gacgatttag aacctatttt
     9901 ccaacacaaa aaaggtgtga gttgggatta cttcgatatt ggattgtttg aatttgatag
     9961 tggttatgta gaggctattg aagcgattgt tgaagcagaa gggcgctttg attttatcta
    10021 tattcaagca ccatactcgg agacattaac gaatttattc caaatgataa gcgaaccata
    10081 caatacgtat gttgatgaat cattttggtc tgttgaatat gaacaagacg aaaatgtcca
    10141 aaaatacgtt gttcaaccat tacattaccg gaacattgaa gaacgtaata ataaattaga
    10201 ggcagttagc ttctcaggac agtatggaga taaggtttct cctaagttag cacttgttca
    10261 tccgaatttt aaaggagatg tcgtttacca aggtaattca gagctcacgt tgagtggaga
    10321 atttggaaaa gaatttaaac ctatcgcatc ttggcaaaat aatctcgttt acgataaaga
    10381 taaagtcatt caaatatggc cagaatttga tattgatggt gcggtcgagt tgcaatatac
    10441 atttagattg attcagactg gcgctgatgg tgcattaatt gagcaaatca tattaactga
    10501 tgatatgtta gacagtcctt tagagatacc tacgaaacca tttgatgcat atataagtgt
    10561 aactgttaag gcgcgtggca acgggacggt acatttagga cctatacaca aacgatggtc
    10621 tagattagac atgggtcaat ttttacttgg tggtagtcgt ttcgtagata gtcaacgaca
    10681 ggaatttatt tattatttcc accctggtga tatgaaacca ccactaaacg tatattttag
    10741 tggttatcga acagcggaag gtttcgaagg atattatatg atgaaacgta tgaatgcacc
    10801 gtttttactt atcggcgatc ctcgtgttga aggtggtagc ttttatatcg gttcatctga
    10861 atatgaacaa ggtattatca atgttattga cgagacatta gaaaagctga attttaaatc
    10921 acatgaatta atattatctg gtttatctat gggttcattt ggtgccttgt attatggtgc
    10981 tcagttaaat cctcaggcaa ttattgttgg taaaccactt gttaatattg gaacgattgc
    11041 tgaacatatg cgattactta gaccagaaga gttcggtaca gcattagacg tcctggtgtc
    11101 taatgaaggt gatacatctc aagcgtcgat acaggcatta aatcaaaaat tttggcaaac
    11161 atttcaaaag aaatcattgt cacaaactgt ttttgccata gcatatatgc aacatgatga
    11221 ttatgatcca catgcatttc aagagctatt acctgtattg acagcacacc aagcaagagt
    11281 gatgaattgc agtattcctg ggcgacataa tgatgattca cctaccattg caagttggtt
    11341 tgttaatttt tacaacatta ttttggaaga caagtttggg agagttcaac atgctgaaaa
    11401 acaaaacatt taaagttact tggaattata tttcacaaac tacatttatg tatggctcta
    11461 aagtatcatt ttcaaatggt gaagtgactt ttataaaccc tttgatgccc tctggattac
    11521 cgattcatga gtggttgatg ttaaagcaat tttcaaaata taaaagtgcg ccaagtttac
    11581 caattttgcg tagaggacag cattataaat tgcattttga ttttgatgca acgcctgctg
    11641 gttcagtgta ttttatcatc attttttata ataaaaatgg cacaaaacta agtactgaga
    11701 ttgtaaagtc aaatagtatc acgattcaat atcctgatga agcctatgct tataaaataa
    11761 aaatgatgaa tgcagcatcg acgagtctta tatttagatg tttaacaatc acagagatga
    11821 cgcatcaaga tgatttagaa tataaaagca tgcgggtgac gaagataggc gatgaccaat
    11881 atggaaatga tatgatcaat gtcattattg cagaaccatc tgacacctat ccaactattt
    11941 cgaatgattt tttgaaactg tttggtcatg tttggctcgt tgaacgttgg atggacgaca
    12001 atatcgaggg aaatattaag caactaaaga atgatttgca aagtcaagat accctaaagg
    12061 ctattaattt gattagttat gggtcaaagt caaatgtttt tgcaacttat gtcgctcagc
    12121 atttggattg caaagtgtat cgtacaagcc atgaagatga tgatttaaaa gaatggctta
    12181 cagaacatgt cccaggaaat catgaactaa aagatacaaa tgtcgaggtt tattttaaag
    12241 aagagcaaga caaccatcta aactatatgt cacgtttaat gaatccagta cgatttttag
    12301 attacttaga tgtatcgaaa ctgaatggtg gggaagtaaa tgaaacataa actagatgtt
    12361 acgataaacg aattaagact aaagtcgatt agaaaaatag tgaaacgcat caatacatgg
    12421 agcgatgaag ttaaatcata ttctgatgat gcgttaaaac aaaagacaat agaatttaaa
    12481 gaacgtttag catcaggagt tgatacacga gatacattgt tgcctgaagc ttatgcagtg
    12541 gcacgtgaag cgagctggag agtattaggt atgtatccta aagaagttca gttaatcgga
    12601 gcgattgtat tgcatgaagg taacattgcg gagatgcaaa caggcgaagg taaaacatta
    12661 acagcaacga tgcctttata tttaaatgca ctttcgggaa aaggaacata tttaattaca
    12721 acgaatgatt atttagcgaa gcgtgatttt gaagagatgc aaccattata tgaatggtta
    12781 ggtttaactg cttcattagg ttttgttgat attgtggatt atgagtatca aaaaggggaa
    12841 aaacgtaata tatatgaaca tgatattata tatacaacta atggacgtct cggttttgat
    12901 tatttaattg ataatttggc tgatagtgcg gaaggaaaat ttttaccaca attaaattac
    12961 ggtattattg atgaagtgga ttcaatcatt ttagatgctg cgcaaacacc attagttatt
    13021 tcgggtgcac caagattaca atcaaatcta tttcatattg tgaaagagtt tgtagataca
    13081 ttgattgaag acgtgcattt taaaatgaag aagaccaaaa aggaaatatg gctgttaaat
    13141 caaggtattg aagcggcaca atcatacttt aatgttgaag atttatatag cgaacaagcg
    13201 atggtcctag tgcgtaatat taatctagca ctgcgcgcgc aatatttgtt tgaatctaat
    13261 gtcgattact tcgtatataa tggtgatatt gttttaattg accgtattac aggtcgtatg
    13321 ttaccgggga ctaagttgca agctggactt caccaagcta ttgaagcgaa agaaggtatg
    13381 gaggtttcaa cagataaaag tgttatggca acaattacct tccagaattt atttaaactt
    13441 tttgaatcat tttcaggtat gacagctaca ggaaaattag gcgaatcaga gttctttgat
    13501 ttgtattcaa aaatagtcgt acaagtacca actgataaag cgattcaacg tatcgatgaa
    13561 ccagataaag tgtttcgttc agttgatgag aaaaacatcg cgatgattca tgatattgtt
    13621 gaacttcatg aaacggggag accggtttta ctcataacga gaactgctga agcggctgaa
    13681 tacttttcga aagtattatt ccaaatggat attcctaata atttactcat tgcgcaaaat
    13741 gttgcaaaag aagcgcagat gatagctgaa gcagggcaaa ttggttccat gactgttgcg
    13801 actagtatgg caggtcgagg cacagatatt aaacttggtg aaggtgtcga agcattaggt
    13861 ggattagctg ttattattca tgaacatatg gaaaatagcc gtgtagacag gcaattacgt
    13921 ggtcgttctg gtagacaagg ggatccggga tcatcttgta tatatatttc actagatgat
    13981 tatttagtta agcgatggag cgatagtaat ttagcggaaa ataatcaatt atattcatta
    14041 gatgcacaac gattatcgca aagtagtttg tttaatcgca aagttaagca aattgtagtt
    14101 aaagcgcagc gtatctcgga agaacaaggg gttaaagctc gtgaaatggc taatgaattt
    14161 gaaaaaagca ttagtataca gcgagatctt gtatacgagg aacgtaatcg agttttagaa
    14221 atagatgatg ctgagaatcg agattttaaa gcgttagcta aagatgtgtt tgaaatgttt
    14281 gtaaatgagg aaaaggtgct aacaaaatcg cgtgttgtcg agtatattta tcaaaattta
    14341 agtttccaat ttaataaaga tgtggcttgt gttaatttta aagataagca agcagtagtg
    14401 acatttttat tagagcaatt tgaaaagcaa ttagctttga atcgtaaaaa catgcaaagt
    14461 gcatattatt ataatatttt cgttcaaaaa gtctttttga aagcaattga ttcatgttgg
    14521 ttagaacaag tcgactattt acaacaatta aaagcaagcg ttaatcaacg acaaaatggg
    14581 caacgtaacg ctatttttga ataccataga gttgccttag attcatttga agttatgaca
    14641 cgaaatatca aaaaaagaat ggtgaaaaat atttgtcaaa gcatgattac atttgataaa
    14701 gagggtatgc cagttataca ttttccataa atgaaaaggg gtagcgcatg acaatatata
    14761 atataaactt tggtataggc tgggcgagta gcggtgttga atatgcacaa gcttaccgtg
    14821 ctaaattatt gagacgtaca aatcaagaca ctaaatttgt gtttttagat tttatacagt
    14881 cagagaacat acagacatta acgcaaaaca ttgggtttaa agatcaagaa gttatttggt
    14941 tgtatcagta ttttagtgat atttctatag caccaacaac gtacacgtta gatgacgttg
    15001 aacgtgagct tggtcatgac attagtttta gagaaagaaa tggtaagata gtccgtctat
    15061 attttaatgg gaaatcatca tttgtaacat gttatctaca aaatgaacaa aaagatgttg
    15121 tggatcgtgc agaatttgta attaatagta tgttagtacg taaagatttt tatagttata
    15181 cgcgtatatt ttctgagtat tatgcaccag ctgataataa agcgaaatta tatatgcgac
    15241 aattctataa tgaagatggt tccatcgctt ataacgaata tattgatgga gacagctcta
    15301 tatatgtgtt tgaagatgcg gtgttgtata acaaaaccga atttattgca tactttttac
    15361 aacgattgaa tttaacccga gacgatattg tcatattaga ccgcgcttct gatattggtc
    15421 aggctgtttt gcagcataaa ggtgatagta aagttggtgt tgtgattcat gctgatcatt
    15481 atagtaataa tatgatgagt gagcagcata tactatggaa caattattat gaatatcaat
    15541 tttcaaaagc taaatacatt gatttcttta ttacagcaac agatattcaa aatcatatgg
    15601 tttgtcgaca atttgagcaa tatcaaggtt atcgcccacg tgtttatact attcctgtgg
    15661 gaagcattga tgcattatca tatccaacat tatcaagaaa gccatatgcg atgattagtg
    15721 catctcgttt agcaaatgag aaacatattg attggcttgt caaagcggtt atcgtggcta
    15781 aacgacaagt gccagaatta acatttgata tttatggaga aggtagcgag aagacgcgac
    15841 tgcgtaaaat tattgataca caccgcgcac aagattatat tcgtttacta gggcatgtta
    15901 aacttgatga aatttataat gattatgagt tatttctttc tgcatcaact agcgaaggtt
    15961 ttgggttaac actaatggaa gcggtaggct caggactagg tatgattgga ttaaatgtga
    16021 attatggtaa cccaaccttt attcgagatg gggaaaatgg ctacttggta ccttttgata
    16081 ccgatgaaga tagtgtggat gatgttgtcg caaaactagc tcatgccatt gtaatgtatt
    16141 ttaacaatgg tccccagaca ccacatgaca tctcttatga agtggcacag caatttatga
    16201 ctcaagatat tattttaaaa tgggaaacat tagttcaaga ggtgctgcat gattaaatta
    16261 tttgattact ttaacgatca ttcacgaaag ttatatgagt cttttaaagc cagtaagttg
    16321 gaagaagatt taacaattgt actcaatgac aatggctttt taccggacga tgttatatca
    16381 ccgtatcaat tttttgcaga taatcataat acagaaaata tgaaaccacg attttttaat
    16441 caggttactg taccagcttt ttgggaaatt aaaggtaaca acaattcggc tacaattaat
    16501 gatatgggac gtctacgagg taaaatattt tatcaaagtg gagagcgccc tagaattgtt
    16561 agtcgtgtcg aatggtttga tgaccaacaa cgtgtccgat ttgtcgatta ttattctaag
    16621 aatggtatta aatttgcaca aactgtttac gatttaaatc gtaaagcgat cttgaagaaa
    16681 tatatgacag cagaaggtaa agaagtgatt tatgaaaatt ttgtaacatc tgatgtcata
    16741 ttggattggc aagggaagtc ttactttttc ccttcaaagc tcgcattcgt attatttttt
    16801 atcaagcaac ttgaaattac ggaacatcat ttcgttatta actcactagc cctaccattt
    16861 tcagtgttat ataatttgcc atcaaatggt agtgatgttt tggtatggca agaacagtgc
    16921 gatggtaatg tcccaggaaa tatgcaattg atgtgtaaag gtgatatgaa acgacattgt
    16981 aatattatta ttccagataa aaacgaatat gaaacgatgc tcaatatagc agacgcaaag
    17041 gttcaatctc gtattttaca aggcggatat ctatataact atcgtagtcg taatagatat
    17101 actaaagaaa ttgtaatatt aactaactcg gatcaacttc gtaacatcaa ggtattggtt
    17161 gaaactttac cagattttaa atttcacatc gctgctatta cagagatgtc tgataagcta
    17221 atgcaacttg atcaatatgc taatgtgcac ttatacccat ctattaatat agacagggta
    17281 aatgaactat accagttatg tgatatttat cttgatatca atgagggtaa tgaaatttta
    17341 aatgcggtgg aacaagcgtt tgactatgag ttgttgattt taggttatcg tcaaacagca
    17401 caccatgcta aagtaacact atcagagcac ttatttgagc ataatgatga gataacaatg
    17461 gagagtaaag accaattaat acaaatgtta gagagtttga aagatcagca acagtttcga
    17521 gatgctttat tagcacaaaa agcacatgca cacgaaatct cgcgtgaaca attcgagcaa
    17581 gtttttaaac aggcgctcga aagctaaaaa gaaaaagcag tctaaaataa agtaccattt
    17641 aatataaaag atttgacgta atatgatgtt agcgacatgg acaatatgat attacgtcat
    17701 gcttgttttg tttacttaaa tataagctaa gtaataagta aataattact aacaacaata
    17761 actagataga taagtgtaaa tttcttgtaa acaggtatat aatagtatgt aattctttta
    17821 aggaagtaag tgagaaagga gtatgttgat ggctaaatat cgagggaaac cgtttcaatt
    17881 atatgtaaag ttatcgtgtt cgacaatgat ggcgacaagt atcattttaa cgaatatctt
    17941 gccgtacgat gcccaagctg catctgaaaa ggatactgaa atttcaaaag agatattatc
    18001 taagcaagat ttattagaca aagttgacaa agcaaatcgt caaattgagc aattaaaaca
    18061 gttatcggct tcatctaaag cacattataa agcacaacta aatgaagcga aaacagcatc
    18121 gcaaatagat gaaatcatta aacgagctaa tgagttggat agcaaagaca ataaaggttc
    18181 tcaaattgaa atgaacggtc aaagtgatat agacagtaaa ttagatcaat tgcttaaaga
    18241 tttaaataag gtttcttcaa aggttgatag gggtcaacaa agtggcaagg acgatcttaa
    18301 tgcaatgaaa aatgatatgt cacaaacggc tacaacaaaa tatggagaaa aagataatga
    18361 aaatgatgaa gcaatggtag ataaggcgtt agaagactta gaccatttga atcagcaaat
    18421 acacaaatcg aaagatgcat cgaaagatac atcggaagat ccagcagtgt ctacaacaga
    18481 taataatcat gaagtagcta aaacgccaaa taatgatggt tctggacatg ttgtgttaaa
    18541 taaatttctt tcaaatgaag agaatcaaag ccatagtaat cgactcactg ataaattaca
    18601 aggaagcgat aaaattaatc atgctatgat tgaaaaattg gctaaaagta atgcctcaac
    18661 gcaacattac acatatcata aactgaatac gttacaatct ttagatcaac gtattgcaaa
    18721 tacgcaactt cctaaaaatc aaaaatcaga cttaatgagc gaagtaaata agacgaaaga
    18781 gcgtataaaa agtcaacgaa atattatttt ggaagaactt gcacgtactg atgataaaaa
    18841 gtatgctaca caaagcattt tagaaagtat atttaataaa gacgaggcag ataaaattct
    18901 aaaagatata cgtgttgatg gtaaaacgga tcaacaaatt gcagatcaaa ttactcgtca
    18961 tattgatcaa ctatctctga caacgagtga tgatttatta acgtcattga ttgatcaatc
    19021 acaagataag tcgctattga tttctcaaat tttacaaacg aaattaggaa aagctgaagc
    19081 agataaattg gctaaagatt ggacgaataa aggattatca aatcgccaaa tcgttaacca
    19141 attgaagaaa cattttgcat caactggcga cacgtcttca gatgatatat taaaagcaat
    19201 tttgaataat gcgaaagata aaaaacaagc aattgaaacg attttagcaa cacgtataga
    19261 aagacaaaag gcaaaattac tggcagattt aattactaaa atagaaacag atcaaaataa
    19321 aatttttaat ttagttaaat cggcattgaa tggtaaagcg gatgatttat tgaatttaca
    19381 aaagagactc aatcaaacga aaaaagatat agactatgtt ttatcaccaa tagtaaatcg
    19441 tccaagttta ctagatcgat tgaataaaaa tgggaaaacg acagatttaa ataagttagc
    19501 aaatttaatg aatcaaggat caaatttatt agacagtatt ccagatatac ccacaccaaa
    19561 gccagaaaag acgttaacac ttggtaaagg taatggattg ttaagtggat tattaaatgc
    19621 tgatggtaat gtatctttgc ctaaagcggg ggaaacgata aaagaacatt ggttgccgat
    19681 atctgtaatt gttggtgcaa tgggtgtact aatgatttgg ttatcacgac gcaataagtt
    19741 gaaaaataaa gcataattat attgggggaa gagcatctat atatttttta agtatataag
    19801 acgtcttatt tctccttaat ttattgtgaa gtatatgcaa aatgcaatga atagattgtc
    19861 catcatttta acgttataat gaatttaacg acttagaact acacaagtaa aggagaatga
    19921 agatgtctcg aaaaacggcg ctattagttt tggatatgca agaaggtata gcgagtagtg
    19981 tacctagaat aaaaaatatt attaaagcga atcagagagc aattgaagca gcaagacaac
    20041 atcgaatacc agtcattttc atacgtttag tgttagataa gcattttaat gatgtctcct
    20101 cgagtaataa agtgttttca acaattaaag ctcaaggata tgcgattact gaagcagatg
    20161 catctacacg aatacttgaa gatttagcac cactagaaga tgagccgatt atttctaagc
    20221 gacgctttag cgcatttaca ggtagttact tggaagttta tttacgtgca aatgatatta
    20281 atcatttagt attaacgggt gtctctacaa gtggagctgt attgagcacg gcattagaaa
    20341 gtgtagataa agactattat attactgttt tagaagatgc tgttggtgat agatcagatg
    20401 ataaacatga ctttattatt gaacaaattt tatcacgctc atgtgacatt gaatccgtag
    20461 agtcatggaa aagtagttta taattaatat aacgtcaatt aaagctcggc agtaatgttt
    20521 gagaataagt acatttgctc atatttataa aatgtgtgag atgacaattg aaacgtatat
    20581 gatgaggaac atttgaacat aaaataatat atttatataa aacgacccga ggcgttcgaa
    20641 ctgaatgcct cgggtttaat tgaataagaa atcggactta tgaacagaaa tatgtttaag
    20701 tccgaactcc ttgtttatac ttataaattt tacgggttta atataatact tatttacctg
    20761 taatatatga taattcttca gcggcagctg cattgatagt tctatgagaa atgataccta
    20821 atcctttaac attggattct gaaataacga tagaaccatc actgttaact ttttcaacaa
    20881 atgctacatg accgtaatgt tgatctgcac caaattgtcc agcctcaaat acaacagcag
    20941 catgacgttt tggtgtatga cttacttgat aatcacggta ttgagctcga ttattccaat
    21001 tatgtgcatc acctaaatca cctgagatag atgtaccaaa ttgtttcata cggttatata
    21061 cgtaccaagt acattggcca tgtggatatg gcatactatc agatacctca cggaaaggtt
    21121 tgaattcatc tgatgaatca tcataatcct tgatagaacg ttcatattta tctaaatctg
    21181 gcatgcgttc atcgtcaaac tgagttaatt gatagtgttt aataatactg tttaatttct
    21241 tagcatagtt tggatctgta gcatatgttt tagataagtg tgatgttgca tctttataag
    21301 aatcggcttc cgatttccat gttggtttat aaattgttcg attgccatca ataccatttt
    21361 taataaggtc agagtaatct tttagtgatt ctttcgtgct tggatatttt cggaatccag
    21421 cattaatact atacaattga ttaccatcag cttctaatgt gttaaaagga acagaattcc
    21481 cttcaaaagc acctttgata ccgaataaat tatggtttgg tgacttagct aaagcactac
    21541 gacctgagtc agattctaag attgcttggg caatcatgac agacgcataa atatcgttat
    21601 cttgaccaat gcgatgtgca tctttagcaa ttgatttgac aaattgacgt gtatcttttg
    21661 agtcaacaac gttaaattgt ccactatcat cattgttaga tatactagga tctgtttcga
    21721 ataatgatgt tgcacgtgta tccttttgat taatatcgtt attgaatgat tgagcaggtt
    21781 tagatttatg tttcaattca tcttgtgttg gtaactgtgg attctttgta ttagattttt
    21841 catttttgtc ttttttagat tgagatgcgt aatctttttg tgttttcttt gcatcttcac
    21901 tgtattgatc caaaatagag tctaaagccg aatctgacat tgactgatta tctttcgatg
    21961 aagatttttg atttgctcta tcgtcacttg ctggttgact atttgattga ttaggttgtg
    22021 ttggctttgg cgaatttggt tgcttattag atgtacttgg ttttgtattg tttgatttag
    22081 gtgctttttg attgtctgct ttatcttgtt tagatgattg cgtatccgtg tcatttttga
    22141 tgctattgtc actgttttta ttcgaatcat ttgttgactt ttcgccatta cgaggttgtt
    22201 cgtaatcaga aatatccgaa tttaaattga ataagttttg gattaaagtt gttaatgagt
    22261 aattatcatc gtatttattt ttggttagca attggtttat attggtttgt ggtaaattct
    22321 tataaataaa atcaatgata ttgttagagc ctgaagtgct gtcgtctata gttttaaatt
    22381 ttttgtcgtt attgtcttgg ctacttgtat tattgttgtc tgctttatca gtatctttac
    22441 ttgtagtatc cttagaagtt tcatcatcat tagatttttt tgaatcatga gatgttgtct
    22501 tagctgtagt atctttttga ggtgtatcag cataagcggt aggtgaaact aaagtaggta
    22561 atacgagcgt agttgatagc aaataaatta aaattttatt tttaggcata tttcgtattc
    22621 tcccttgaaa aatataataa ttaagtgtga taataaacta tgatttgtta taatttatcg
    22681 tatgctgtaa atagttgata ggtatcaatc gactaaatat cttccagtaa attgattata
    22741 ctaattcaca acgcaaaaat aaattaattt acaaaaaata tataaaaaat atgaataatt
    22801 cctacatagg agtgtgacaa tgaagaacgc atttaaatta tttaaaatgg atctgaagaa
    22861 agtagctaag acgccagctg tgtggattat cttagcaggc ttagctattt tgccatcgtt
    22921 ctacgcttgg tttaacttat gggcaatgtg ggatccatat ggcaacacgg gacacatcaa
    22981 ggtcgcagtc gttaatgaag ataaaggcga cacaatcaga gggaaaaaag ttaatgtcgg
    23041 taatacgatg gttaatacac tcaagaaaaa taaaagtttt gattggcagt ttgtaagtag
    23101 agagaaagct gatcatgaga taaaaatggg taaatatttt gcaggtattt acatcccatc
    23161 taagtttaca catgaaatta ccgggacact acgtaagcag cctcaaaaag cagatgtaga
    23221 atttaaggtg aatcagaaga ttaacgctgt tgcgtctaag ctaacagata ctggttcgtc
    23281 agttgtcgtt gaaaaagcga atgaacaatt taataaaaca gtaactcgag cattattaga
    23341 agaagctaac aaagcaggtt taactattga agaaaatgtg ccgacaatta ataagataaa
    23401 aaatgcggta tattcagcag ataaagcttt acctaagatt aatgactttg cgaataaaat
    23461 tgtatatttg aataaccacc aagcggattt agataaatat gccaatgatt ttagaaaact
    23521 aggaaattat aaaggcgata ttttagatgc tcagaaaaaa ttaaacgaag tcaatggtgc
    23581 tattccgcaa cttaatgaaa aggctaagtt gatattagct ttaaataatt atatgccgaa
    23641 aattgaaaaa gcgttaaatt ttgcagctga tgacgtgcca gcgcagttcc ctaaaattaa
    23701 tcaaggactt aacattgcga gtcaaggtat tgatcaagct aatggacagt taaatgatgc
    23761 caaagacttc gtcacacaag ttagaagtag agtcggtgat tatcaagatg caattcgacg
    23821 cgcgcaagat ttaaatcgaa gaaaccagca acagattcct caaaatagcg cggcgaacaa
    23881 cgaaacatca aatagtgcac ctgcagctgg taatggtgta gcatcaacgc caccaagtgt
    23941 accaagtggc gatactgcac caaataataa tgttacgcaa aataccgcac caaatggtaa
    24001 taatgcgcct gtatcgacta caccacaaag tacaagcggg aaaaaagatg gtcaaagttt
    24061 tgcagatata acaacaacac aagtcagcac agctaacgag aacacacaaa acattacaga
    24121 taaagatgtt aaatcaatgg aagcggcatt aacgggctct ttattatcat tatcaaataa
    24181 tttagatacc caagcgaaag ccgcacaaaa agatagtcag gcattacgta atatttcgta
    24241 tggcatttta gcatcggaca agccttctga ttttagagag tctttagata atgttaagtc
    24301 cggtttagaa tacacaacgc aatataatca acaatttatc gatacattaa aagagattga
    24361 gaagaatgaa aatgttgatt tatcaaaaga aattgataag gtaaaggcag ctaataatcg
    24421 aattaatgaa tcattaaggt tagttaatca attaagcaat gcattaaaga atggtagttc
    24481 aggaactgct gaagctacta aattactaga tcaactttca aaactagatt catcattatc
    24541 atcatttaga gattatgtta aaaaagatct taacagctct ttagtatcag tatcacaacg
    24601 tattatggat gaattgaaca aagggcaaac tgcattatcc aatgttcagt ctaaattaaa
    24661 tacaattgat caagtcatta atagtggaca agctatttta aaaaatggta aaacacgtat
    24721 tgatcgctta caaacagtat taccaagtat tgaacaacaa tacattagtg ctgttaaaaa
    24781 tgctcaagca aacttcccga aagtgaaaag tgatgtagct aaagctgcta actttgtgcg
    24841 caatgactta ccacagttgg aacagcgatt aactaatgcg acagcaagtg tgaataaaaa
    24901 tttaccaacg ttattaaatg gttatgatca agcagtagga ttactaaata aaaatcagcc
    24961 acaagcgaaa aaggctttat cagatttagc tgatttttct caaaataaat tgcctgatgt
    25021 tgaaaaagat ttgaaaaaag cgaataagat tttcaagaag ttagacaaag acgatgcagt
    25081 cgacaaatta atcgacacac ttaagaatga tttaaaaaaa caagcgggta ttattgcaaa
    25141 tcctattaat aagaagactg ttgatgtttt cccagttaag gattatggtt caggtatgac
    25201 accattctat actgcactgt cagtatgggt aggtgcactc ttgatggtaa gcctattaac
    25261 tgttgataat aaacataaga gcttagagcc agtgttaacg acacgacaag tgttcttagg
    25321 taaggcagga ttctttataa tgcttggtat gttgcaagcg ctcattgtat cggttggaga
    25381 tttgttaatc ctaaaagcag gggttgagtc acctgtatta ttcgtactta taacgatttt
    25441 ctgttcgatt attttcaact cgatcgtata tacgtgcgta tcattacttg gtaacccagg
    25501 taaagccatt gcaatcgtat tgcttgtatt acaaattgca ggtggtggag gaacattccc
    25561 aattcaaact acgccacaat ttttccaaaa catttcgcca tacttaccat ttacgtatgc
    25621 aattgattca ttacgtgaaa cagtaggcgg tattgttccg gaaatcttaa ttacaaaatt
    25681 aattatatta acgttatttg gcataggatt cttcgttgta ggtttaattt taaaacctgt
    25741 aacagatcca ttgatgaaac gcgtatctga aaaagttgac caaagtaacg ttacagaata
    25801 aaaatgaaat ccacacatta gggttatagc tccttaatgt gtggattttt atgtttttag
    25861 acagaagaga tagtaatttc tgtcttttat gggacgggtg ttatcattgc tattatccag
    25921 gatgacttac tatagcacta atattaccga caaagtgaat atcctcgtct tccgtagtta
    25981 aaataaagct agaacctttt gggatgtcat agtgcttatc gtttactgtt aaagtaccag
    26041 taccatcgat aattgtaact aagcaataag catgtggttt attgaatttt aaatctccat
    26101 gaatatccca tttatatact gcaaaatatt gattatctac aaattgagtt acagtgtgtg
    26161 tgtcgatgtg agttgttata ggagtagtat ttggttcatg attgcctaat tcaatcacat
    26221 ctttactttg ctctaagtgc aaatcacgca attgaccatt ttgatctcgt ctatcatagt
    26281 cataaatacg gtatgtcgta tcggaggatt gttgtgtctc taaaattaaa atacccgaac
    26341 caatggcatg gacagtgcca gcaggaacat aataaaagtc accgggctta acaggtatac
    26401 gtttgaaaag actgtcaaat tcatgattat caatcatgtc tattaacgtc tgtttattat
    26461 gtgcatgtac gccatatata atttcagcac ctggggctgc atctaaaata taccaacatt
    26521 ctgttttacc tagttcgcct tcgtgtttta aagcgtaatc atcatccggg tgaacttgaa
    26581 cagataattt atcattggca tctaatactt tagttagcag agggaaacta tctcgtgaat
    26641 cattatcgaa taattcacga tgttgtgacc aaagttgatc tagggtcata tccttgtatg
    26701 gaccattgat aattgtatta ggaccatttg gatgtgcaga aattgcccag cattcaccag
    26761 ttgtttcatt agggatatca tagttaaatg cttttaatgc atgaccgccc caaattctgt
    26821 ctttaaaaac gggttgtaaa aataatgcca tagttaaaac tcctctatat tttcattaat
    26881 aagttataaa tttctgtagt actgtttgca ttaattagtg attggcgtgt ctcatcattc
    26941 attaacgctt tagataagcg ctgaagtatt tttaaatgtg tatcctgact gttgtttggt
    27001 acggcaatta agaatatcaa ttgaggtaga ctaccatcta gactctccca tttaacacca
    27061 tgattatttt tcataacagc tacaatcggt tgttttacaa catcagactt tgcatgtgga
    27121 atggccacgt tcatgccaat agctgtcgta gactccattt cacgttctag gattgcattt
    27181 tttaaatgcg atgtgtgctc tacataacgg caaattttaa gtttatgaat caacatatca
    27241 attgcttcgt ttcgagacat gtcgtgatca gtaattatca tagtttgttg atcaaaaaca
    27301 tgagaaggtt tattgtgatg tgaatgtttc gcggtgttat ctacattgtc aacctctgta
    27361 tcatgttgtg ttatatctgt atcatgaagt tgcgtgtgtt gcgctggtgc atctactgct
    27421 ataactggtg tattgcgttt taataatagt acagtagtca ttgtgacaag actacctact
    27481 atcactgcaa agataaacaa taatacatga tcaataccac ctaatacagc cacgattgga
    27541 cctccatgtg cgactctatc gccgacacca ccaatggctg caatgactga tgcaatcatt
    27601 gcaccaatga tgtttgcagg tataatgcgc aatggatctt gggctgcgaa aggaatagca
    27661 ccttcagtaa taccaaatag tcccatagtg aaggaagcct tacccatttc tctttcggaa
    27721 tgattgaatt tatacttttg aacaaacgtt gctaaaccta aaccgattgg tggtgtacat
    27781 acagcaactg cgaccatacc cataacggcg taattacctt cagcaataag tgctgagcca
    27841 aataaaaatg ctactttgtt tactggaccg cccatatcga aggcaatcat cgcacctata
    27901 atcatcgcaa gtataataat attagcacct tgcatacttt ttaaccaggt tgttaatgcc
    27961 tcaaaaatat tagaaattgg tgcaccgatt aaaaatataa atatcaatcc tacaacgacc
    28021 gatgaaataa tgggaataat aatgataggc ataattggtg ccattgcttt tggaacttta
    28081 atatctttaa tccactttgc gatataacct gctaagaaac cagcaacaat accacctaaa
    28141 aatcctgcgc ctgcatcact gccataaaaa ctaccgtcag cagcgatagc gccgccaatc
    28201 ataccaggaa caagaccggg cttgtcagcg atactaacag cgatatatcc agctagtatt
    28261 ggaaccataa atttaaaggc taaactacca atgttttcaa tggatttcca aaatgaatca
    28321 tctgggatga ctaatccttt tgatgtcgtt tcaccgccta gagtcagcgc gatggcgata
    28381 aggagtccac caactacgat aaaaggaacc ataaacgata caccgttcat taaatgttga
    28441 tacaccattt gaataccact tttagactta ccgcgatctt tcgaatgata atttgtttca
    28501 gattgataaa taggcgcatc ttgattaatg atacgttgaa ttagacctct cggattatga
    28561 atcccttcgc gaacattttc attaatcaac cgtttaccaa caaatcggga cagatcaact
    28621 tgtttatcag ctgcaattat gacaccatca gcttcttcga tgtcttgcgt agttaaaaca
    28681 ttttcagcac caacaccgcc ttgtgtctct actttaatat ccacacccat ttcttttgct
    28741 acctgctcaa gcttttcttg agccatatat gtatgtgcaa tgccatttgg gcatgaggta
    28801 atagctacaa ttttcataaa atcatctcct tttctatatt gtaagcgcat tctcgatact
    28861 aaaaaaaaga ataattaccg ttactagtgg caattattct tgtaagtatt caaataactg
    28921 ttgctttaaa ctatgatcat ctaaattaca taaatggttc actgaatcat catccaagtt
    28981 agcaattaat tgcatcattt gttttgtaaa agctgtgtct ttttgtgaaa ttgctaagaa
    29041 aaagacaagt ttgacatcgt gttgtcgcca aggaaaaaca tcttttgtgc gaaaaataag
    29101 cacatgtgat tgtaaaactt tttcaggatc accatgagga atcgccataa aattacctat
    29161 gtatgtagaa gatgatttct cacgctctaa agctgattcg atatatcctt ctacaatcgc
    29221 atgatgtgct tgtaatattt tttgagcttc ttcaaaaatt tgcacagtat gtcgtggttt
    29281 ttgttcagta tttacgacaa ggaaattgac agtgtccata tgatgatgtg cttgaaccgg
    29341 attttgcttt tgcttcacaa tatgtctgat tttgtgacgg tcatcctcag aaaataatgg
    29401 tgcaaccttg atagttgaaa ggtgcttagg aagtatgttt agcgtttgtt ttggaatatc
    29461 atgggtcgtt attaataaat ctacattgtc aaagtgatag tgtgttatat tttctagttt
    29521 aatcgtattt atcactaaca actcttcgga taagttattt attttagttt ctaaaaaatt
    29581 cgacacacct agaccataat aacaagcaat gactacattt aattgtgttt tggtacgacg
    29641 ctcgatggca gcttgaaaat gaatagttaa aaaagcaatt tcatcttcac tcatctcaat
    29701 atctgtatca actgctaatt tatcaatcgc ttcaaaaagt gtgttaaaca caaagggata
    29761 gagtttttta atctctataa ctaaaggatt gtttaaataa atgttttgag tgatacgtaa
    29821 atatgcttta ctaaaatgat tatataaatt ttgttgtaaa atcgaatctt cattgaaagg
    29881 tacatgaata cgttgctgca tcaattcgat taagcgatca atataacttt gtataaatat
    29941 acgttctatg ccaatatcga gtttattaaa atgataagca ataaagaatg aaaacatatt
    30001 gattacttgt tcgttcaagt cataacctaa tctttcgttg atttgcttaa tgcaagattg
    30061 agatatcaat tttgcatcgt ggtttaacgt gtctagctca cttaaagatt gcttacgttt
    30121 aatgattaaa atgagatgaa ttaaaagctg ttgtatttga atatcagttg tttcaatact
    30181 atgttgttga agtgtctctt gtataatatg cgaaatcatc ctttggtgtg aatcaggtaa
    30241 ttcatttaaa attaggtctt caacatgtac atgccctgat gataattgat ttaaatggat
    30301 gatggcatta gtgatatcat tatctgttcc atcgaccaca ataccttgtt ttttcttaat
    30361 tgttaaagcg atattaaatt tttgacacca ttgttgaata cgaatgaaat agtcgttgag
    30421 cacagtttta gataatagat aatcttgctc tagttgttgc aatgtctgcg atttattgtg
    30481 cattagcagt tgatacgcca tagataataa tagtttttct ttatatgatt gaatatgcgt
    30541 gtaacgttca gttatcgttt ctaaggtata ctgcgatgta tttaattgat atcctttaga
    30601 tttaatactt acgatgatat catccataaa attgctattg ataacatgga tatcattgcg
    30661 aactgtgcgg tttgaaacat tgacatgttc agcaatttca ttagaactaa tgtgctttga
    30721 tggattttta ataaaaaact ggagtagttt taagtgtcta tcaagcattt aacatgtacc
    30781 tcctttctaa attttccgtg taagcgtttt taaaggtgta ttaattttat taaacatgag
    30841 agcttataca tacgtcaatg acattaaagc gaacttttat atgaatttaa cagagtgcga
    30901 attatgcaaa taaagaacag cagtaagata tttcaaatag aaaaatatct tactgctgtt
    30961 tttctgaatt tatgtacctg atacttgtgg acgtatcagg taaatgatta atttttaggt
    31021 gattgtactt gaggtgtttg tttagaaggc gtattggtat tatttgattt attagcaggt
    31081 ggtgtgttct gttgtttatg attttggtta ttcgttgaag gttgactgct accgccgtga
    31141 tttgaatttg attgattcgg attagattga tttgaatttg attgattcgg attagatggt
    31201 tgtgtattcg ctggtgctgt tgaaccgtta ttttggacag ttgatggcgt agatgattga
    31261 ttgccattag taacaccgtt gttgttttga ttttcatttg tgttgtttgt attttcgtct
    31321 ttttgatgat ttacttcagt atctttcttt tgatgggtag ctttattatc ttgctcatta
    31381 ttatgatttg acgatgtaga atgatttatt gcagcataaa tggcgaatcc aagtgcacca
    31441 attaacaaga tgataacaac agtaataaga aattttttca aatgtaacgc ctccgtttat
    31501 aataaaaata gtcatacatt gcatggcatc atgctttcaa tgtcgacaaa atgatgttaa
    31561 ctataaaact tgaaatgcgg acgatattaa tgtttgtgtt agtttttatg tacttcaaat
    31621 aagtataaca aaactgttat atatttataa atataatcaa tagtatttta ttgtacaagc
    31681 aagttataat aatagtaata agattgaaaa atttgtatgg aaaagagaga taaatatgaa
    31741 tcaaacaact aaactttgtg cagcaacgac attagcatta ggtactttaa ttggcgcaac
    31801 agttgtaggg actgcatcac caatacatca agaaacgcag gcggcaacaa caccttacta
    31861 tacttatcat ggatatatcg gtcataacgc taattttata atggataagc attttattaa
    31921 cgctattaaa tatgacaacg tgacatttaa tggtattaaa ttagcgaaga caaatgcgac
    31981 aaaaaaagta gagaaatacg atcaaacttt taaaggtgta actgcaaaag gaaatgaagc
    32041 gaatcaatta caatttatcg ttaaaaataa tatatcacta aaagatattc aaaaagctta
    32101 tggtaaagat ttgaaaaaag agaataataa aacgaaagaa tctgatagcg gtatttttta
    32161 ttatcaacat tctaaaaagt cattaggcat ttggtttgtt gttgatcata atagagtggt
    32221 tgaagttaca gtgggacata cgccatacaa aacgagtaaa taataaagta aatatagggg
    32281 ctagtttgtg aggaatgatg tcacatacta gtctttttga ttatatgatg gtagttttcg
    32341 tgtctccatg atattgaaat atatagattt aatcatgcgc agttaagtag tcagcatata
    32401 tatttcacgt attgattaag tatgtgttgt actaattttt tgattgttaa taatttcaca
    32461 aaaataaagc cttgataaat tttaaaatat aattaagtgc
//
LOCUS       DAHHOJ010000021        30597 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.021, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000021 DAHHOJ010000000
VERSION     DAHHOJ010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30597
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.021"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            141..1595
                     /locus_tag="NBH32_000803"
     CDS             141..1595
                     /locus_tag="NBH32_000803"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCD1514427.1"
                     /translation="MSDRIRVRYAPSPTGYLHIGNARTALFNYLYAKHYNGDFVIRIE
                     DTDKKRNLEDGETSQFDNLKWLGLDWDESVDKDNGYGPYRQSERQHIYQPLIDQLLAE
                     DKAYKCYMTEEELEAEREAQIARGEMPRYGGQHAHLTEEQRQQFEAEGRQPSIRFRVP
                     QNQTYSFDDMVKGNISFDSNGIGDWVIVKKDGIPTYNFAVAIDDHYMQISDVIRGDDH
                     ISNTPKQIMIYEAFGWEPPRFGHMSLIVNEERKKLSKRDGQILQFIEQYRDLGYLPEA
                     LFNFIALLGWSPEGEEEIFSKEEFIKIFDEKRLSKSPAFFDKQKLAWVNNQYMKQKDT
                     ETVFQLALPHLIKANLIPEVPSEEDLSWGRKLIALYQKEMSYAGEIVPLSEMFFKEMP
                     ALGEEEQQVINGEQVPELMTHLFSKLEVLEPFEAAEIKKTIKEVQKETGIKGKQLFMP
                     IRVAVTGQMHGPELPNTIEVLGKEKVLNRLKQYK"
     gene            2018..2665
                     /gene="cysE"
                     /locus_tag="NBH32_000804"
     CDS             2018..2665
                     /gene="cysE"
                     /locus_tag="NBH32_000804"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCD1514428.1"
                     /translation="MILLKRMRDDIKMVFEQDPAARSTLEVITTYAGLHAVWSHLIAH
                     KLYNQKKYVAARAISQISRFFTGIEIHPGAKIGKRLFIDHGMGVVIGETCTIGDNVTI
                     YQGVTLGGTGKERGKRHPDIGDNVLIAAGAKVLGNIKINSNVNIGANSVVLQSVPSYS
                     TVVGIPGHIVKQDGVRVGKTFDHRHLPDPIYEQIKHLERQLEKTRNGEIQDDYII"
     gene            2649..4049
                     /locus_tag="NBH32_000805"
     CDS             2649..4049
                     /locus_tag="NBH32_000805"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000631968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCD1514429.1"
                     /translation="MITLYNTLTRQKEVFKPIEPGKVKMYVCGPTVYNYIHIGNARPA
                     INYDVVRRYFEYQGYNVEYVSNFTDVDDKLIKRSQELNQSVPEIAEKYIAAFHEDVGA
                     LNVRKATSNPRVMDHMDDIIQFIKDLVDQGYAYESGGDVYFRTRKFEGYGKLSHQSID
                     DLKVGARIDAGEHKEDALDFTLWKKAKPGEISWDSPFGEGRPGWHIECSVMAFHELGP
                     TIDIHAGGSDLQFPHHENEIAQSEAHNHAPFANYWMHNGFINIDNEKMSKSLGNFILV
                     HDIIKEVDPDVLRFFMISVHYRSPINYNLELVESARSGLERIRNSYQLIEERAQIATN
                     IENQQTYIDQIDAILNRFETVMNDDFNTANAITAWYDLAKLANKYVLENTTSTEVIDK
                     FKAVYQIFSDVLGVPLKSKNADELLDEDVEKLIEERNEARKNKDFARADEIRDMLKSQ
                     NIILEDTPQGVRFKRG"
     gene            4042..4446
                     /locus_tag="NBH32_000806"
     CDS             4042..4446
                     /locus_tag="NBH32_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HCD1514430.1"
                     /translation="MDNQQDNHIKLLNPLTLAYMGDAVLDQYVRTYIVLKLKSKPNKL
                     HQMSKKYVSAKSQAQTLEYLMEQEWFTDEEMDILKRGRNAKSHTKAKNTDVQTYRKSS
                     AIEAVIGFLYLEKREERLEALLNKIITIVNER"
     gene            4454..5200
                     /gene="rlmB"
                     /locus_tag="NBH32_000807"
     CDS             4454..5200
                     /gene="rlmB"
                     /locus_tag="NBH32_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCD1514431.1"
                     /translation="MEDTVIVGRHAVREAIITGHPINKILIQEGIKKQQINEILKNAK
                     DQKIIVQTVPKSKLDFLANAPHQGVAALIAPYEYADFDQFLKQQKEKEGLSTVLILDG
                     LEDPHNLGSILRTADATGVDGVIIPKRRSVTLTQTVAKASTGAIEHVPVIRVTNLAKT
                     IDELKDNGFWVAGTEANNATDYRNLEADMSLAIVIGSEGQGMSRLVSDKCDFYIKIPM
                     VGHVNSLNASVAASLMMYEVFRKRHDVGEI"
     gene            5200..5724
                     /locus_tag="NBH32_000808"
     CDS             5200..5724
                     /locus_tag="NBH32_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HCD1514432.1"
                     /translation="MKERYLIIDGYNMIGQSPTLSAIAKENLEEARIQLIDAIANYNA
                     VISDEIICVFDAYDQSGVEREYMYHGVKTIFTKEKETADSFIERYVYELYDKHTKHIT
                     VVTSDMSEQHAIFGSGAYRISSREMWRDLKENEIDVSKSLDDISENKPRTRIPLSSEI
                     LAEFEKIRRGHHKK"
     gene            5805..6374
                     /locus_tag="NBH32_000809"
     CDS             5805..6374
                     /locus_tag="NBH32_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HCD1514433.1"
                     /translation="MKYDLTTQDSTIKRNNAINDKDFEKLVMDLQPLIIRRIKTFGFN
                     HYDLEDLYQEILIRMYRSVQTFDFSGEQPFTNYVQCLITSVKYDYLRKYLATNKRMDN
                     LINEYRVTYPCAIKRFDVENNYLNKLAIKELICQFKYLSAFEKDVMYLMCEQYKPREI
                     AQLMHVKEKVIYNAIQRCKNKIKRYFKMI"
     gene            6489..6632
                     /gene="rpmG"
                     /locus_tag="NBH32_000810"
     CDS             6489..6632
                     /gene="rpmG"
                     /locus_tag="NBH32_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002451211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1514434.1"
                     /translation="MRKIPLNCEACGNRNYNVPKQEGSATRLTLKKYCPKCNAHTIHK
                     ESK"
     gene            6688..6870
                     /gene="secE"
                     /locus_tag="NBH32_000811"
     CDS             6688..6870
                     /gene="secE"
                     /locus_tag="NBH32_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCD1514435.1"
                     /translation="MAKKESFFKGVKSEMEKTSWPTKEELFKYTVIVVSTVIFFLVFF
                     YALDLGITALKNLLFG"
     gene            6883..7431
                     /gene="nusG"
                     /locus_tag="NBH32_000812"
     CDS             6883..7431
                     /gene="nusG"
                     /locus_tag="NBH32_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCD1514436.1"
                     /translation="MSEEVGAKRWYAVHTYSGYENKVKKNLEKRVESMNMTEQIFRVV
                     IPEEEENQVKDGKAKTTVKKTFPGYVLVELIMTDESWYVVRNTPGVTGFVGSAGAGSK
                     PNPLLPEEVRFILKQMGLKEKTIDVELEVGEQVRIKSGPFANQVGEVQEIETDKFKLT
                     VLVDMFGRETPVEVEFDQIEKL"
     gene            7612..8034
                     /gene="rplK"
                     /locus_tag="NBH32_000813"
     CDS             7612..8034
                     /gene="rplK"
                     /locus_tag="NBH32_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012664221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCD1514437.1"
                     /translation="MAKKVDKVVKLQIPAGKANPAPPVGPALGQAGVNIMGFCKEFNA
                     RTQDQAGLIIPVEISVYEDRSFTFITKTPPAPVLLKKAAGIEKGSGEPNKTKVGTVTK
                     DQVREIANSKMQDLNAADEEAAMRIIEGTARSMGIVVE"
     gene            8242..8934
                     /gene="rplA"
                     /locus_tag="NBH32_000814"
     CDS             8242..8934
                     /gene="rplA"
                     /locus_tag="NBH32_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCD1514438.1"
                     /translation="MAKKGKKYQEAASKVDRTQHYSVEEAIKLAKETSIANFDASVEV
                     AFRLGIDTRKNDQQIRGAVVLPNGTGKSQSVLVFAKGDKIAEAEAAGADYVGEAEYVQ
                     KIQQGWFDFDVVVATPDMMGEVGKLGRVLGPKGLMPNPKTGTVTMDVKKAVEEIKAGK
                     VEYRAEKAGIVHASIGKVSFTDEQLIENFNTLQDVLAKAKPSSAKGTYFKSVAVTTTM
                     GPGVKIDTASFK"
     misc_feature    9022..9168
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            9206..9706
                     /locus_tag="NBH32_000815"
     CDS             9206..9706
                     /locus_tag="NBH32_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCD1514439.1"
                     /translation="MSAIIEAKKQLVDEIAEVLSNSVSTVIVDYRGLTVAEVTDLRSQ
                     LREAGVEYKVYKNTMVRRAAEKAGIEGLDEFLTGPTAIATSSEDAVAAAKVISGFAKD
                     HEALEIKSGVMEGNVITAEEVKTVGSLPSHDGLVSMLLSVLQAPVRNFAYAVKAIGEQ
                     KEESAE"
     gene            9749..10117
                     /gene="rplL"
                     /locus_tag="NBH32_000816"
     CDS             9749..10117
                     /gene="rplL"
                     /locus_tag="NBH32_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001273587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCD1514440.1"
                     /translation="MANHEQIIEAIKEMSVLELNDLVKAIEEEFGVTAAAPVAVAGAA
                     GGADAAAEKTEFDVELTSAGSSKIKVVKAVKEATGLGLKDAKELVDGAPKVIKEALPK
                     EEAEKLKEQLEEVGATVELK"
     gene            10292..10900
                     /locus_tag="NBH32_000817"
     CDS             10292..10900
                     /locus_tag="NBH32_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1514441.1"
                     /translation="MSHYYDEDPSVISNEQRIQYQLNHHKIDLITDNGVFSKDKVDYG
                     SDVLVQTFLKAHPPGPSKRIADVGCGYGPIGLMIAKVSPHHSITMLDVNHRALALVEK
                     NKKINGIDNAIVKESDALSAVEDKSFDFILTNPPIRAGKETVHRIFEQALHRLDSNGE
                     LFVVIQKKQGMPSAKKRMNELFGNVEVVNKDKGYYILRSIKA"
     gene            11115..14666
                     /gene="rpoB"
                     /locus_tag="NBH32_000818"
     CDS             11115..14666
                     /gene="rpoB"
                     /locus_tag="NBH32_000818"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCD1514442.1"
                     /translation="MAGQVVQYGRHRKRRNYARISEVLELPNLIEIQTKSYEWFLREG
                     LIEMFRDISPIEDFTGNLSLEFVDYRLGEPKYDLEESKNRDATYAAPLRVKVRLIIKE
                     TGEVKEQEVFMGDFPLMTDTGTFVINGAERVIVSQLVRSPSVYFNEKIDKNGRENYDA
                     TIIPNRGAWLEYETDAKDVVYVRIDRTRKLPLTVLLRALGFSSDQEIVDLLGDNEYLR
                     NTLEKDGTENTEQALLEIYERLRPGEPPTVENAKSLLYSRFFDPKRYDLASVGRYKTN
                     KKLHLKHRLFNQKLAEPIVNTETGEIVVEEGTVLDRRKIDEIMDVLESNANSEVFELH
                     GSVIDEPVEIQSIKVYVPNDDEGRTTTVIGNAFPDSEVKCITPADIIASMSYFFNLLS
                     GIGYTDDIDHLGNRRLRSVGELLQNQFRIGLSRMERVVRERMSIQDTESITPQQLINI
                     RPVIASIKEFFGSSQLSQFMDQANPLAELTHKRRLSALGPGGLTRERAQMEVRDVHYS
                     HYGRMCPIETPEGPNIGLINSLSSYARVNEFGFIETPYRKVDLDTHAITDQIDYLTAD
                     EEDSYVVAQANSKLDENGRFMDDEVVCRFRGNNTVMAKEKMDYMDVSPKQVVSAATAC
                     IPFLENDDSNRALMGANMQRQAVPLMNPEAPFVGTGMEHVAARDSGAAITAKHRGRVE
                     HVESNEILVRRLVEENGVEHEGELDRYPLAKFKRSNSGTCYNQRPIVAVGDVVEYNEI
                     LADGPSMELGEMALGRNVVVGFMTWDGYNYEDAVIMSERLVKDDVYTSIHIEEYESEA
                     RDTKLGPEEITRDIPNVSESALKNLDDRGIVYIGAEVKDGDILVGKVTPKGVTELTAE
                     ERLLHAIFGEKAREVRDTSLRVPHGAGGIVLDVKVFNREEGDDTLSPGVNQLVRVYIV
                     QKRKIHVGDKMCGRHGNKGVISKIVPEEDMPYLPDGRPIDIMLNPLGVPSRMNIGQVL
                     ELHLGMAAKNLGIHVASPVFDGANDDDVWSTIEEAGMARDGKTVLYDGRTGEPFDNRI
                     SVGVMYMLKLAHMVDDKLHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGA
                     AYTLQEILTYKSDDTVGRVKTYEAIVKGENISRPSVPESFRVLMKELQSLGLDVKVMD
                     EQDNEIEMTDVDDDDVVERKVDLQQNDAPETQKEVTD"
     gene            14830..18426
                     /gene="rpoC"
                     /locus_tag="NBH32_000819"
     CDS             14830..18426
                     /gene="rpoC"
                     /locus_tag="NBH32_000819"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCD1514443.1"
                     /translation="MKIGLASPEKIRSWSFGEVKKPETINYRTLKPEKDGLFCERIFG
                     PTKDWECSCGKYKRVRYKGMVCDRCGVEVTKSKVRRERMGHIELAAPVSHIWYFKGIP
                     SRMGLLLDMSPRALEEVIYFASYVVVDPGPTGLEKKTLLSEAEFRDYYDKYPGQFVAK
                     MGAEGIKDLLEEIDLDEELKLLRDELESATGQRLTRAIKRLEVVESFRNSGNKPSWMI
                     LDVLPIIPPEIRPMVQLDGGRFATSDLNDLYRRVINRNNRLKRLLDLGAPGIIVQNEK
                     RMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRFRQNLLGKRVDYSGRSV
                     IAVGPSLKMYQCGLPKEMALELFKPFVMKELVQREIATNIKNAKSKIERMDDEVWDVL
                     EEVIREHPVLLNRAPTLHRLGIQAFEPTLVEGRAIRLHPLVTTAYNADFDGDQMAVHV
                     PLSKEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYYLTLERKDAVNTGAIFN
                     NTNEVLKAYANGFVHLHTRIGVHASSFNNPTFTEEQNKKILATSVGKIIFNEIIPDSF
                     AYINEPTQENLERKTPNRYFIDPTTLGEGGLKEYFENEELIEPFNKKFLGNIIAEVFN
                     RFSITDTSMMLDRMKDLGFKFSSKAGITVGVADIVVLPDKQQILDEHEKLVDRITKQF
                     NRGLITEEERYNAVVEIWTDAKDQIQGELMQSLDKTNPIFMMSDSGARGNASNFTQLA
                     GMRGLMAAPSGKIIELPITSSFREGLTVLEYFISTHGARKGLADTALKTADSGYLTRR
                     LVDVAQDVIVREEDCGTDRGLLVSDIKEGTEMIEPFIERIEGRYSKETIRHPETDEII
                     IRPDELITPEIAKKITDAGIEQMYIRSAFTCNARHGVCEKCYGKNLATGEKVEVGEAV
                     GTIAAQSIGEPGTQLTMRTFHTGGVAGSDITQGLPRIQEIFEARNPKGQAVITEIEGV
                     VEDIKLAKDRQQEIVVKGANETRSYLASGTSRIIVEIGQPVQRGEVLTEGSIEPKNYL
                     SVAGLNATESYLLKEVQKVYRMQGVEIDDKHVEVMVRQMLRKVRIIEAGDTKLLPGSL
                     VDIHNFTDANREAFKHRKRPATAKPVLLGITKASLETESFLSAASFQETTRVLTDAAI
                     KGKRDDLLGLKENVIIGKLIPAGTGMRRYSDVKYEKTAKPVAEVESQTEVTE"
     gene            18563..18817
                     /locus_tag="NBH32_000820"
     CDS             18563..18817
                     /locus_tag="NBH32_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal L7Ae/L30e/S12e/Gadd45 family protein"
                     /protein_id="HCD1514444.1"
                     /translation="MSKEKVARFNKQHFVVGLKETLKALKKDQVTSLIIAEDVEVYLM
                     TRVLSQINQKNIPVSFFKSKHALGKHVGINVNATIVALIK"
     gene            18915..19328
                     /gene="rpsL"
                     /locus_tag="NBH32_000821"
     CDS             18915..19328
                     /gene="rpsL"
                     /locus_tag="NBH32_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001142337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCD1514445.1"
                     /translation="MPTINQLVRKPRQSKIKKSDSPALNKGFNSKKKKFTDLNSPQKR
                     GVCTRVGTMTPKKPNSALRKYARVRLSNNIEINAYIPGIGHNLQEHSVVLVRGGRVKD
                     LPGVRYHIVRGALDTSGVDGRRQGRSLYGTKKPKN"
     gene            19394..19864
                     /gene="rpsG"
                     /locus_tag="NBH32_000822"
     CDS             19394..19864
                     /gene="rpsG"
                     /locus_tag="NBH32_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013054923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCD1514446.1"
                     /translation="MPRKGSVPKRDVLPDPIHNSKLVTKLINKIMLDGKRGTAQRILY
                     SAFDLVEQRSGRDALEVFEEAINNIMPVLEVKARRVGGSNYQVPVEVRPERRTTLGLR
                     WLVNYARLRGEKTMEDRLANEILDAANNTGGAVKKREDTHKMAEANKAFAHYRW"
     gene            19987..22068
                     /gene="fusA"
                     /locus_tag="NBH32_000823"
     CDS             19987..22068
                     /gene="fusA"
                     /locus_tag="NBH32_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCD1514447.1"
                     /translation="MAREFSLEKTRNIGIMAHIDAGKTTTTERILYYTGRIHKIGETH
                     EGASQMDWMEQEQDRGITITSAATTAAWEGHRVNIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFVNKMDKLGANFEYSVSTLHDRLQANAA
                     PIQLPIGAEDEFEAIIDLVEMKCFKYTNDLGTEIEEIEIPEDHLDRAEEARASLIEAV
                     AETSDELMEKYLGDEEISVSELKEAIRQATTNVEFYPVLCGTAFKNKGVQLMLDAVID
                     YLPSPLDVKPIIGHRASNPEEEVIAKADDSAEFAALAFKVMTDPYVGKLTFFRVYSGT
                     MTSGSYVKNSTKGKRERVGRLLQMHANSRQEIDTVYSGDIAAAVGLKDTGTGDTLCGE
                     KNDIILESMEFPEPVIHLSVEPKSKADQDKMTQALVKLQEEDPTFHAHTDEETGQVII
                     GGMGELHLDILVDRMKKEFNVECNVGAPMVSYRETFKSSAQVQGKFSRQSGGRGQYGD
                     VHIEFTPNETGAGFEFENAIVGGVVPREYIPSVEAGLKDAMENGVLAGYPLIDVKAKL
                     YDGSYHDVDSSEMAFKIAASLALKEAAKKCDPVILEPMMKVTIEMPEEYMGDIMGDVT
                     SRRGRVDGMEPRGNAQVVNAYVPLSEMFGYATSLRSNTQGRGTYTMYFDHYAEVPKSI
                     AEDIIKKNKGE"
     gene            22285..23469
                     /gene="tuf"
                     /locus_tag="NBH32_000824"
     CDS             22285..23469
                     /gene="tuf"
                     /locus_tag="NBH32_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCD1514448.1"
                     /translation="MAKEKFDRSKEHANIGTIGHVDHGKTTLTAAIATVLAKNGDSVA
                     QSYDMIDNAPEEKERGITINTSHIEYQTDKRHYAHVDCPGHADYVKNMITGAAQMDGG
                     ILVVSAADGPMPQTREHILLSRNVGVPALVVFLNKVDMVDDEELLELVEMEVRDLLSE
                     YDFPGDDVPVIAGSALKALEGDAQYEEKILELMEAVDTYIPTPERDSDKPFMMPVEDV
                     FSITGRGTVATGRVERGQIKVGEEVEIIGLHDTSKTTVTGVEMFRKLLDYAEAGDNIG
                     ALLRGVAREDVQRGQVLAAPGSITPHTEFKAEVYVLSKDEGGRHTPFFSNYRPQFYFR
                     TTDVTGVVHLPEGTEMVMPGDNVEMTVELIAPIAIEDGTRFSIREGGRTVGSGVVTEI
                     IK"
     gene            complement(23751..24926)
                     /locus_tag="NBH32_000825"
     CDS             complement(23751..24926)
                     /locus_tag="NBH32_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1514449.1"
                     /translation="MLDWFQLANNKENKTIQLRRYLHQYPELSFEEFQTHDYIVNQLS
                     QLSCDIETPIGRNGIKATFKGLGTGPTIALRADFDALPVEELNDVPYKSKNPGCMHAC
                     GHDGHTAILLTVAEILDEHKHLLEGNVVLIFQYGEEIMPGGSQEMIDAGCLENVDRIY
                     GTHLWSGYPTGTIHSRAGAIMASPDEFSVTIKGRGGHGAKPHETIDPIVIMAEFILSA
                     QKIISRTIDPVKQAVLSFGMIQAGTTDSVIPDQAFCKGTVRTFDSDIQNHVMDKMDKL
                     LQGLAIANDINYDLNYIKGYLPVHNNEKAYQVIKEATNDLHVRFNESDLMMIGEDFSH
                     YLKVRPGAFFLTGCGNESKGITAPHHNPKFDIDEKSLKYAVAVFLKIIELEQVFKTN"
     gene            25097..26284
                     /locus_tag="NBH32_000826"
     CDS             25097..26284
                     /locus_tag="NBH32_000826"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCD1514450.1"
                     /translation="MVQSLHEFLEENINYLKENGLYNEIDTIEGANGPEIKINGKSYI
                     NLSSNNYLGLATNEDLKSAAKAAIDTHGVGAGAVRTINGTLDLHDELEETLAKFKGTE
                     AAIAYQSGFNCNMAAISAVMNKNDAILSDELNHASIIDGCRLSKAKIIRVNHSDMDDL
                     RAKAKEAVESGQYNKVMYITDGVFSMDGDVAKLPEIVEIAEEFGLLTYVDDAHGSGVM
                     GKGAGTVKHFGLQDKIDFQIGTLSKAIGVVGGYVAGTKELIDWLKAQSRPFLFSTSLA
                     PGDTKAITEAVKKLMDSTELHDKLWDNAQYLKNGLSKLGYDTGESETPITPVIIGDEK
                     TTQEFSKRLKDEGVYVKSIVFPTVPRGTGRVRNMPTAAHTKDMLDEAIAAYEKVGKEM
                     KLI"
     gene            26551..27429
                     /gene="hchA"
                     /locus_tag="NBH32_000827"
     CDS             26551..27429
                     /gene="hchA"
                     /locus_tag="NBH32_000827"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCD1514451.1"
                     /translation="MSQDVNELSKQPTPDKAEDNAFFPSPYSLSQYTATKTDFDGVEH
                     KGAYKDGKWKVLMIAAEERYVLLENGKMFSTGNHPVEMLLPLHHLMEAGFDVDVATLS
                     GYPVKLELWAMPTEDEAVISTYNKLKEKLKQPKKLADVIKNELGPDSDYLSVFIPGGH
                     AAVVGISESEDVQQTLDWALDNDRFIVTLCHGPAALLSAGLNREKSPLEGYSVCVFPD
                     SLDEGANIEIGYLPGRLKWLVADLLTKQGLKVVNDDMTGRTLKDRKLLTGDSPLASNE
                     LGKLAVNEMLNAIQNK"
     gene            27587..29224
                     /locus_tag="NBH32_000828"
     CDS             27587..29224
                     /locus_tag="NBH32_000828"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCD1514452.1"
                     /translation="MSYSIGIDYGTASGRVFLINTTNGQVVSKFVKPYTHGVIESELN
                     GLKIPYTYALQNSNDYLEIMEEGISYIVRESKIDPVNIVGIGIDFTSSTIIFTDENLN
                     PVHNLKQFKNNPHAYVKLWKHHGAYKEAEKLYQTAIENNNKWLGHYGYNVSSEWMIPK
                     IMEVMNRAPEIMEKTAYIMEAGDWIVNKLTNKNVRSNCGLGFKAFWEEETGFHYDLFD
                     KIDPKLSKVIQDKVSAPVVNIGEVVGKLDDKMAQKLGLSKETMVSPFIIDAHASLLGI
                     GSEKDKEMTMVMGTSTCHLMLNEKQHQVPGISGSVKGAIIPELFAYEAGQSAVGDLFE
                     YVAKQAPKSYVDEAANRNMTVFELMNEKIKHQMPGESGLIALDWHNGNRSVLSDSNLT
                     GCIFGLTLQTKHEDIYRAYLEATAFGTKMIMQQYQDWHMEVEKVFACGGIPKKNAVMM
                     DIYANVLNKKLIVMDSEYAPAIGAAILGAVSGGAHNSINDAVDAMKEPILYEINPEAE
                     KVQRYETLFKAYKALHDIHGYKKANIMKDIQSLRVEG"
     gene            29438..30403
                     /locus_tag="NBH32_000829"
     CDS             29438..30403
                     /locus_tag="NBH32_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1514453.1"
                     /translation="MKKIMITGALGQIGTELVVKCREIYGTDNVLATDIREPEADSPV
                     QNGPFEILDVTDRDRMFELVRDFEADSLMHMAALLSATAEKNPILAWDLNMGGLMNAL
                     EAARTYNLHFFTPSSIGAFGDSTPKVNTPQVTIQQPTTMYGVNKVAGELLCQYYFKRF
                     GVDTRSVRFPGLISHVKEPGGGTTDYAVEIYFKAVREGHYTSFIDKGTYMDMMYMDDA
                     IEAIIKLMEADDAKLETRNGYNLSAMSFDPEMVKEAIQEYYPNFTLDYDVDPIRQGIA
                     NSWPDSIDTSCSRGEWGFDPKYDLASMTKLMLEAIEQKDTVKNNN"
BASE COUNT        10893 a         4586 c         6023 g         9095 t
ORIGIN      
        1 ttttaaggtc atgttaaaaa gcattgtttc gttttaacaa taagacgagt tagacatttg
       61 ttatgacggt cgaagatagc aaatgaacaa tttatatgaa ctaacttgaa aatctgatat
      121 ttaatttagg agtgaaaaaa atgagcgatc gtataagagt aagatatgca ccaagtccaa
      181 ctggttatct tcatattggt aatgcaagaa cagcattatt caattacttg tatgctaaac
      241 attacaacgg agattttgtg attcgaattg aagatactga taaaaaacgt aatttagaag
      301 atggagaaac atcacaattt gataatctta aatggttagg attagattgg gatgagtctg
      361 tagataaaga caatggctac ggaccatatc gtcaatctga acgtcaacat atctaccaac
      421 cattaataga tcagttacta gcagaagata aagcatataa atgctatatg acagaagaag
      481 aattagaagc tgaacgtgaa gcgcaaatcg ctcgtggtga aatgcctcgc tatggtggtc
      541 aacatgcgca tttgactgaa gaacaacgtc aacaatttga agcagaagga cgccaaccat
      601 caattcgttt ccgagtacct caaaaccaaa cgtattcatt tgatgatatg gtaaaaggaa
      661 atatttcatt tgattcaaat ggtattggtg actgggttat cgtaaaaaaa gatggcattc
      721 caacgtacaa ttttgcagta gctatagatg atcattacat gcaaatttca gatgtaattc
      781 gtggtgatga tcatatttca aacacgccta aacaaattat gatttatgaa gcatttggct
      841 gggagccacc tcgttttggt catatgtcat taattgttaa tgaagaacgt aaaaagttaa
      901 gtaaacgtga tgggcaaatt ttacaattta ttgagcaata tcgtgactta ggttatttac
      961 ctgaagcgtt atttaatttt attgcgttat taggttggtc tcctgaaggt gaagaagaaa
     1021 tcttttctaa agaagaattt atcaaaatct ttgatgaaaa gcgtttgtca aaatcaccag
     1081 catttttcga taagcaaaaa ttagcatggg ttaataacca atatatgaaa caaaaagata
     1141 ctgaaacagt attccaatta gcattacctc atttaattaa agcaaatttg attcctgagg
     1201 tgccgtcaga agaggattta tcttggggac gcaaattaat tgcgctttat caaaaagaaa
     1261 tgagttatgc cggtgaaatt gtacctttat cagaaatgtt ctttaaagaa atgccagctc
     1321 ttggtgaaga agaacaacaa gtgattaatg gagagcaagt accagagtta atgacgcact
     1381 tattcagtaa attagaagta cttgaaccat ttgaagcggc tgaaattaaa aagacaatta
     1441 aagaagttca aaaagaaaca ggaataaaag gcaagcaatt atttatgcct attcgtgttg
     1501 ctgtaacagg ccaaatgcat ggtcctgaat taccaaatac aattgaagta cttggtaaag
     1561 aaaaagtgct aaaccgttta aaacaatata agtaatgaaa tagacaagat caaggtagta
     1621 tatacttgaa ttttataacg taaccacata tgataaagtt gtattaacaa attacaacaa
     1681 tatgaataaa gtattttata taaaacgaag gattagtaat taaatttata cgatgcagag
     1741 agtgtacggt tgctgtgagt acaacgtaga aattaatgaa tgcaccttcg taaaatgaat
     1801 taaatatata atgagagtga tgagcattaa gttgacttag tttccttgat aatttggaag
     1861 cgcccgcaat attattaatg ttattcgcta aattcagagt ggaaccgtgc ggaagcgcct
     1921 ctaacaatac aatttgtatg ttagtggtgc ttttttgata tttaatttcg caggtacttc
     1981 aattatgtat ttacgtagtt caatcattga ggaggaaatg atcttgttaa aaagaatgag
     2041 agacgatata aaaatggtat ttgagcagga tccagcggca cgttcaacat tagaagtcat
     2101 tacaacgtat gcaggtttac atgcagtttg gagtcatttg attgcacata agttatacaa
     2161 ccaaaaaaaa tatgttgcag cacgcgcgat atctcaaatt tcaagatttt tcacaggtat
     2221 agaaatccat ccaggtgcta aaattggaaa gcgtctattt atagatcatg gtatgggcgt
     2281 tgtaatagga gaaacatgta caattggtga taatgtgaca atctatcaag gcgtgacact
     2341 tggtgggaca gggaaagaaa gagggaaaag acacccagat ataggagaca atgttttaat
     2401 agcagccggt gcgaaagttt taggaaatat taaaataaat tcaaatgtaa atattggtgc
     2461 aaattcagtt gttttacaat cagttccaag ttattcaacg gttgttggta taccaggaca
     2521 tattgttaag caagatggtg ttcgagttgg aaaaacattt gatcatcgcc atctacctga
     2581 tccaatttat gaacaaatta agcatttaga acgacaactt gaaaagacta ggaatggaga
     2641 gattcaagat gattacatta tataatacgc ttacacgtca aaaagaagtg ttcaagccta
     2701 tagaaccagg gaaagtaaaa atgtatgtat gtggtcctac tgtatataac tacattcata
     2761 ttggtaacgc aagaccagca attaattatg acgtagtgag acgttacttt gaataccaag
     2821 gatataatgt agaatatgta tcaaatttta cagacgtaga tgataaatta attaaacgtt
     2881 ctcaagaatt aaatcagtct gttcccgaaa ttgcagaaaa atatatcgct gcttttcatg
     2941 aagatgttgg tgcgttaaat gttagaaaag cgacttcaaa tccaagggta atggaccata
     3001 tggatgacat tattcaattt attaaagatt tggtggatca aggttatgca tatgaaagtg
     3061 gtggcgatgt ttactttaga acacgtaaat ttgaaggtta tggtaaatta agtcatcaat
     3121 ccatagatga cttaaaagtg ggtgctcgta tagatgcagg agagcataaa gaagatgcac
     3181 ttgattttac attgtggaaa aaagcgaagc ctggcgagat tagttgggat agcccatttg
     3241 gtgaaggtag accaggatgg catatagaat gttctgtaat ggcatttcat gagctaggac
     3301 ctacaattga tatacatgcg ggtggttcag atttacaatt tccacatcat gaaaatgaaa
     3361 tagcacaatc agaagcacat aatcatgcgc catttgctaa ttattggatg cataatggtt
     3421 tcattaatat tgataatgaa aaaatgagta aatcactagg caactttatt ttagttcacg
     3481 atattattaa agaagttgat ccagatgtac taagattctt tatgattagc gtacattata
     3541 gaagcccaat taactataat ctagaattgg tagaatcagc acgtagtgga ctagagcgta
     3601 ttcgcaatag ttatcaatta attgaagagc gcgcacaaat tgctactaat attgaaaatc
     3661 aacagacata tattgatcaa attgatgcga ttttaaatcg ttttgaaaca gttatgaatg
     3721 atgattttaa tacagctaat gcaattacag cttggtatga tttagcaaaa cttgcgaata
     3781 aatatgtact agagaataca acatcaacag aagtaattga taaatttaaa gcagtttatc
     3841 aaattttcag cgatgtttta ggtgtaccgt taaaatctaa aaatgcagat gaattattgg
     3901 atgaagatgt tgaaaaatta atcgaagagc gtaatgaagc aaggaaaaac aaagattttg
     3961 cacgagcaga tgaaattcga gacatgctga aatcacaaaa cattatatta gaagacacac
     4021 ctcaaggggt tagatttaaa cgtggataat caacaagata atcacattaa attattgaat
     4081 ccattgacct tagcatatat gggagacgca gtcttagatc aatatgtacg tacctatatc
     4141 gttttaaagc ttaaaagtaa gcctaataaa ctacatcaaa tgtctaaaaa atatgtatct
     4201 gccaaaagtc aggcgcaaac gttagaatat ttaatggagc aagaatggtt tacagacgaa
     4261 gaaatggata ttttgaagcg agggcgtaac gcgaaaagtc atactaaagc taaaaacact
     4321 gatgttcaaa catatcgtaa aagttcagcg atagaagcag tgataggttt tctttattta
     4381 gaaaaaagag aagaacgatt agaggcatta ttaaataaaa taataacaat agtaaacgaa
     4441 aggtagtgac aatgtggaag atacggttat tgttggtagg catgctgtta gagaagcgat
     4501 tattactggg catccgataa ataagatatt gattcaagaa ggtattaaaa agcaacaaat
     4561 taatgaaatt ttaaaaaatg caaaagatca aaaaatcatt gttcaaactg taccaaaatc
     4621 taaattagat tttttagcaa atgcaccaca tcagggtgtt gcagcgctta ttgcaccata
     4681 tgaatatgct gacttcgatc aatttttaaa acagcaaaaa gaaaaagaag gtttatcgac
     4741 agtacttata ttagacggct tagaagaccc gcataacttg ggatcaattt taagaacagc
     4801 cgatgcaacg ggagttgatg gtgttattat tcctaaacgt cgttcagtta cactaacgca
     4861 aacagttgca aaagcctcaa caggtgcaat tgaacatgta ccagttattc gagtgacaaa
     4921 tttagctaaa actatcgatg aactaaaaga taatggcttt tgggtagctg gcactgaagc
     4981 taataatgca acagattata gaaatctaga agcggacatg tcattggcta ttgtaattgg
     5041 tagcgaagga cagggtatga gtcgcctagt aagtgataaa tgcgattttt atattaagat
     5101 tccaatggtt ggacatgtaa acagtttgaa tgcttcggtt gcagcaagtt taatgatgta
     5161 cgaagtattt cgaaaaagac atgatgttgg agaaatataa tgaaagaacg ttacttaatc
     5221 attgatggat acaatatgat aggacaatca ccaacgctaa gcgccattgc aaaagagaat
     5281 ttagaagaag ctagaataca attaatagat gcaattgcaa attataatgc agttatttca
     5341 gatgaaatta tttgtgtttt cgatgcttat gaccaatcgg gtgttgaaag agaatacatg
     5401 tatcatggcg ttaaaacgat ttttaccaag gaaaaagaaa cagctgatag tttcatagaa
     5461 cgttatgttt atgaacttta tgacaagcat actaagcata ttacagttgt aacaagtgat
     5521 atgagtgagc aacatgctat ctttggatca ggtgcatata gaatatcatc tcgcgaaatg
     5581 tggagagatt taaaagaaaa tgaaattgat gtgagtaaat cattagatga tataagtgaa
     5641 aacaagccaa gaactcgaat tccgttatct tctgaaatcc ttgcagaatt tgaaaaaata
     5701 cgaagaggac atcataagaa atgacatttc cgtcatcttg aaattttgaa tgtaacaata
     5761 ttaaactaac cttaaatttt agatagaagg ggttagttta atacttgaaa tatgatttga
     5821 caactcaaga cagtacaatc aaacgtaaca atgcaataaa tgataaagac ttcgaaaagt
     5881 tagtaatgga tctacaacca ttaattattc gacgcatcaa aacatttgga tttaatcatt
     5941 atgatttaga agacttatat caagaaatac ttatacggat gtataggtcg gtccaaacat
     6001 ttgattttag tggagagcag cctttcacaa attatgttca atgtttaatt acgtctgtaa
     6061 agtatgatta tttgagaaaa tatttagcta caaataaaag aatggataat ttgattaatg
     6121 aatatagagt tacgtatcca tgtgcaataa agcgttttga tgttgaaaac aattatttga
     6181 ataaattagc aattaaagag ttgatttgtc agtttaagta tttgagtgca tttgaaaaag
     6241 atgtcatgta tttaatgtgt gaacaatata agccgagaga aattgctcaa ttgatgcatg
     6301 taaaagagaa agtgatttat aatgccatac aacgatgtaa aaataaaata aaacgttatt
     6361 tcaaaatgat ttgaaaagcg ccttaggacg tgaattgaat tataacgtgt tacttactga
     6421 tggtttgaca tttgttataa attttatgta tagtatactg gtattataat gaataaaggt
     6481 gaattattgt gagaaaaata cctttaaatt gtgaagcttg tggcaataga aattataatg
     6541 ttcctaagca agaaggctcg gcaacaagat taaccttaaa gaaatattgt ccaaaatgta
     6601 acgcgcacac aattcataaa gaatcgaaat aaatacattc gaaataatac tttgataata
     6661 tgttcaaagg atttggaggt tgagcagatg gctaaaaaag aaagtttctt taaaggcgtt
     6721 aagtctgaaa tggaaaaaac aagttggccg acgaaagaag agctatttaa atatactgta
     6781 attgtagttt ctactgttat attcttctta gtctttttct atgccttaga tttaggaatt
     6841 acagcattga aaaatttatt atttggttag aggagtgaag acatgtctga agaagttggc
     6901 gcaaagcgtt ggtatgcagt gcatacatat tctggatatg aaaataaagt taaaaagaat
     6961 ttagaaaaaa gagtagaatc tatgaatatg actgaacaaa tctttagagt agtcataccg
     7021 gaagaagaag aaaatcaagt aaaagatggc aaagctaaaa cgactgttaa aaaaacattc
     7081 cctggatatg tcttagtgga attaatcatg acagatgaat catggtatgt ggtaagaaat
     7141 acaccaggtg ttactggttt tgtaggttct gcaggtgcag ggtctaagcc aaatccattg
     7201 ttaccagaag aagttcgctt catcttaaaa caaatgggtc ttaaagaaaa gactatcgat
     7261 gttgaactcg aagttggcga gcaagttcgt attaaatcag gtccatttgc gaatcaagtt
     7321 ggtgaagttc aagaaattga aacagataag tttaagctaa cagtattagt agatatgttt
     7381 ggccgagaaa caccagtaga agttgaattc gatcaaatag aaaagcttta attaacaatt
     7441 aaagttatta aactaaccaa aagataaaaa agagtattga ttttttaatt agaaaagtgt
     7501 taaaattatg tggtcgcgct tttagagcgc ccatttcgtc acgaaatgtt aagagtggga
     7561 gggcaaaact gagccctgtg accacatcac gatatcaagg aggtgcacat cgtggctaaa
     7621 aaagtagata aagttgttaa attacaaatt cctgcaggta aagcgaatcc agcaccacca
     7681 gttggtccag cattaggtca agcaggtgtg aacatcatgg gattctgtaa agagttcaat
     7741 gcacgtactc aagatcaagc aggtttaatt attccggtag aaatcagtgt ttatgaagat
     7801 cgttcattta catttattac aaaaactcca ccggctccag tattacttaa aaaagcagct
     7861 ggtattgaaa aaggttcagg cgaaccaaac aaaactaaag ttggtacagt aactaaagat
     7921 caagtacgcg aaattgctaa cagcaaaatg caagacttaa acgctgctga cgaagaagca
     7981 gctatgcgta ttatcgaagg tactgcacgt agtatgggta tcgttgtaga ataattttac
     8041 gaatattaaa tttgattaca tgatttaaac gatgaagcag ataacagaga taataatgat
     8101 gaattataaa tataatctga atgactagat taatgattga tttattcata agattaattc
     8161 ttctgttgtc tgctcttaac ttgcatatag caagtaatgt gggaggaaat tccgctaaaa
     8221 ccactaaagg aggaactata aatggctaaa aaaggtaaaa agtatcaaga agcagctagt
     8281 aaagttgacc gtactcagca ctacagtgtt gaagaagcaa ttaaattagc taaagaaaca
     8341 agcattgcta actttgacgc ttctgttgaa gttgcattcc gtttaggaat tgatacacgt
     8401 aaaaatgacc aacaaatccg tggtgcagtt gtattaccaa acggaactgg taaatcacaa
     8461 agtgtattag tattcgctaa aggtgacaaa attgctgaag ctgaagcagc aggtgctgac
     8521 tatgtaggtg aagcagaata cgttcaaaaa atccaacaag gttggttcga cttcgatgta
     8581 gtagttgcta caccagacat gatgggtgaa gttggtaaat taggtcgtgt attaggacca
     8641 aaaggtttaa tgccaaaccc taaaactgga actgtaacaa tggatgttaa aaaagctgtt
     8701 gaagaaatca aagctggtaa agtagaatat cgtgctgaaa aagctggtat cgtacatgca
     8761 tcaattggta aagtttcatt tactgatgaa caattaattg aaaacttcaa tactttacaa
     8821 gatgtattag ctaaagctaa accatcatct gctaaaggta catacttcaa atctgttgct
     8881 gtaactacaa caatgggtcc tggagttaaa attgatactg caagtttcaa ataataaata
     8941 atataaacaa ttacaggctg aaagaaatat ctttcagtct gtaaaaatat attgacaata
     9001 agtaatttcc aagttatatt acttattgtg attattttac ctaagacagt aggagttatt
     9061 tataacttaa aatttatcct gccgaggcta aaattgactt gaacgtgatg atctatgatc
     9121 tttcaagcac tttttgccgt gggtagaaag tgcttttttt attaatttta aaaaaagcac
     9181 caaaaattta aatggaggtg tctgaatgtc tgctatcatt gaagctaaaa aacaactagt
     9241 tgatgaaatt gctgaggtac tatcaaattc agtttcaaca gtaatcgttg actaccgtgg
     9301 attaacagta gctgaagtta ctgacttacg ttcacaatta cgtgaagctg gtgttgagta
     9361 taaagtatac aaaaacacta tggtacgtcg tgcagctgaa aaagctggta tcgaaggctt
     9421 agatgaattc ttaacaggtc ctactgctat tgcaacttca agtgaagatg ctgtagctgc
     9481 agcgaaagta atttctggat ttgctaaaga tcatgaagca ttagaaatta aatcaggcgt
     9541 tatggaaggc aatgttatta cagcagaaga agttaaaact gttggttcat taccttcaca
     9601 cgatggtctt gtatctatgc ttttatcagt attacaagct cctgtacgca acttcgctta
     9661 tgcggttaaa gctattggag aacaaaaaga agaaagcgct gaataatttt tagcgtaaaa
     9721 aaattaaaaa taatggagga attataaaat ggctaatcat gaacaaatca ttgaagcgat
     9781 taaagaaatg tcagtattag aattaaacga cttagtaaaa gcaattgaag aagaatttgg
     9841 tgtaactgca gctgctccag tagcagtagc aggtgcagct ggtggcgctg acgctgcagc
     9901 agaaaaaact gaatttgacg ttgagttaac ttcagctggt tcatctaaaa tcaaagttgt
     9961 taaagctgtt aaagaagcaa ctggtttagg attaaaagat gctaaagaat tagtagacgg
    10021 agctcctaaa gtaatcaaag aagctttacc taaagaagaa gctgaaaaac ttaaagaaca
    10081 attagaagaa gttggagcta ctgtagaatt aaaataattc aagtatctta aacttaataa
    10141 tcaaagtttt atagcaagta ttgctataat ataatgattc tttgagaagt taaaaccccg
    10201 ttattttgat aacggggttt tattcattta aagactgagt gaaatgttat aattataatg
    10261 acgagttaca aagtgaagat gaggtggaat aatgagtcat tattacgatg aagatccaag
    10321 tgtaattagc aatgaacaac gtattcaata tcaattaaac catcataaaa ttgatttaat
    10381 aactgataat ggagtgtttt cgaaagataa agtagattat ggttcagatg ttcttgttca
    10441 aactttttta aaagcgcatc cacctggtcc aagtaagcga attgccgatg ttggttgtgg
    10501 ttacggacca attggtttga tgattgctaa agtatcacca catcattcaa ttacaatgct
    10561 agatgttaat cacagagcgc tagccttagt tgaaaaaaac aaaaaaataa atggtattga
    10621 taatgcgatc gtaaaggaaa gtgatgcttt gtctgctgtg gaagacaaaa gttttgattt
    10681 tattttaacc aatccaccaa taagagcagg gaaagaaacc gtgcatcgta tattcgagca
    10741 agcattacat agattagact cgaacggtga actattcgtt gtaattcaga agaagcaagg
    10801 tatgccatct gcaaagaaaa gaatgaatga actttttgga aatgtagaag tggtaaataa
    10861 agataaagga tattacattc tgagaagtat aaaagcttga aatgaaatgg atattctgtt
    10921 atagttatac aatgtaaaaa tttatgttca ataagtgtgt acttttacgt taaatagata
    10981 agttaattaa gaataaatat agaatcgaaa atggtgtcat cattaatgtt gccgttttct
    11041 ttttgtcttt ttattaatat gcttatggta tttagctaaa agcggatcac ataatttttg
    11101 aggggtgaat ctgtttggca ggtcaagttg tccaatatgg aagacatcgt aaacgtagaa
    11161 actacgcgag aatttcagaa gtattagaat taccaaactt aatagaaatt caaactaaat
    11221 cttacgagtg gttcctaaga gaaggtttaa tcgaaatgtt tagagacatt tctccaattg
    11281 aagattttac tggtaatttg tcattagagt ttgtggatta ccgtttagga gaaccaaaat
    11341 atgatttaga agaatctaaa aaccgtgacg ctacttatgc tgcacctctt cgtgtaaaag
    11401 tgcgtctaat cattaaagaa acaggagaag ttaaagaaca agaagtcttt atgggtgatt
    11461 tcccattaat gactgataca ggtacgttcg ttatcaatgg tgcagaacgt gtaatcgtat
    11521 ctcaattagt tcgttcacca tccgtttatt tcaatgaaaa aatcgacaaa aatggtcgtg
    11581 aaaactatga tgcaacaatt attccaaacc gtggtgcatg gttagaatat gaaacagatg
    11641 ctaaagatgt tgtatacgtg cgtattgata gaacacgtaa actaccatta acagtattgt
    11701 tacgtgcatt aggtttctca agcgaccaag aaattgttga ccttttaggt gacaatgaat
    11761 atttacgtaa tactttagag aaagacggca ctgaaaacac tgaacaagcg ttattagaaa
    11821 tctatgaacg tttacgtcca ggtgaaccac caactgttga aaatgctaaa agtctattgt
    11881 attcacgttt ctttgatcca aaacgctatg acttagcaag cgtgggtcgt tataaaacaa
    11941 acaaaaaatt acatttaaaa catcgtttat ttaatcaaaa attagctgag ccaattgtaa
    12001 atactgaaac tggtgaaatt gtagttgaag aaggtacagt gcttgatcgt cgtaaaatcg
    12061 acgaaatcat ggatgtactt gaatcaaatg caaacagcga agtgtttgaa ttgcatggta
    12121 gcgttataga cgagccagta gaaattcaat caattaaagt atatgttcct aacgatgatg
    12181 aaggtcgtac gacaactgta attggtaatg ctttccctga ctcagaagtt aaatgcatta
    12241 caccagcaga tatcattgct tcaatgagtt acttctttaa cttattaagc ggtattggat
    12301 atacagatga tattgaccat ttaggtaacc gtcgtttacg ttctgtaggt gaattactac
    12361 aaaaccaatt ccgtatcggt ttatcaagaa tggaaagagt tgtacgtgaa agaatgtcaa
    12421 ttcaagatac tgagtctatc acacctcaac aattaattaa tattcgacct gttattgcat
    12481 ctattaaaga attctttggt agctctcaat tatcacaatt catggaccaa gcaaacccat
    12541 tagctgagtt aacgcataaa cgtcgtctat cagcattagg acctggtggt ttaacacgtg
    12601 aacgtgctca aatggaagta cgtgacgttc actactctca ctatggccgt atgtgtccaa
    12661 ttgaaacacc tgagggacca aacattggat tgattaactc attatcaagt tatgcacgtg
    12721 taaatgaatt cggctttatt gaaacaccat atcgtaaagt tgatttagat acacacgcta
    12781 tcactgatca aattgactat ttaacagctg acgaagaaga tagctatgtt gtagcacaag
    12841 caaactctaa attagatgaa aatggtcgtt tcatggatga tgaagttgta tgtcgtttcc
    12901 gtggtaacaa cacagttatg gctaaagaaa aaatggatta tatggatgta tcgccgaagc
    12961 aagttgtttc agcagcgaca gcatgtattc cattcttaga aaatgatgac tcaaaccgtg
    13021 cattgatggg tgcgaacatg caacgtcaag cagtgccttt gatgaatcca gaagcaccat
    13081 ttgttggtac aggtatggaa cacgttgcag cacgtgattc tggtgcggct attacagcta
    13141 agcacagagg tcgtgttgaa catgttgaat ctaatgaaat tcttgtacgt cgtctagttg
    13201 aagagaacgg cgttgagcat gaaggtgaat tagatcgcta tccattagct aaatttaaac
    13261 gttcaaactc aggtacatgt tataaccaac gtccaatcgt tgcagttgga gatgttgttg
    13321 agtataacga gattttagca gacggaccat ctatggaatt aggagaaatg gcattaggta
    13381 gaaacgtagt agttggtttc atgacttggg acggttacaa ctatgaggat gccgttatca
    13441 tgagtgaaag acttgtgaaa gatgacgtgt atacttctat tcatattgaa gagtatgaat
    13501 cagaagcacg tgatactaag ttaggacctg aagaaatcac aagagatatt cctaatgttt
    13561 ctgaaagtgc acttaagaac ttagacgatc gtggtatcgt ttatattggt gcagaagtaa
    13621 aagatggaga tattttagtt ggtaaagtaa cgcctaaagg tgtaactgag ttaactgccg
    13681 aagaaagatt gttacatgca atctttggtg aaaaagcacg tgaagttaga gatacttcat
    13741 tacgtgtacc tcacggcgct ggcggtatcg ttcttgatgt aaaagtattc aatcgtgaag
    13801 aaggcgacga tacattatca cctggtgtaa accaattagt acgtgtatac atcgttcaaa
    13861 aacgtaaaat tcatgttggt gataagatgt gtggtcgaca tggtaacaaa ggtgtcattt
    13921 ctaagattgt tcctgaagaa gatatgcctt acttaccaga cggacgtccg atcgatatca
    13981 tgttaaaccc tcttggtgta ccatctcgta tgaacatcgg acaagtatta gagctacact
    14041 taggtatggc tgctaaaaat cttggtattc acgttgcatc acctgtattt gacggtgcaa
    14101 acgatgacga tgtatggtca acaattgaag aagctggtat ggctcgtgat ggtaaaactg
    14161 tactttatga tggacgtaca ggtgaaccat tcgataaccg tatttcagta ggtgtaatgt
    14221 acatgttgaa acttgcgcac atggttgatg ataaattaca tgcgcgttca acaggaccat
    14281 attcacttgt tacacaacaa ccacttggcg gtaaagcgca attcggtgga caacgtttcg
    14341 gtgagatgga ggtatgggca cttgaagcat atggtgctgc atacacatta caagaaatct
    14401 taacttacaa atccgatgat acagtaggac gtgtgaaaac atacgaggct attgttaaag
    14461 gtgaaaacat ctctagacca agtgttccag aatcattccg agtattgatg aaagaattac
    14521 aaagtttagg tttagatgta aaagttatgg atgagcaaga taatgaaatc gaaatgacag
    14581 acgttgatga cgatgatgtt gtagaacgca aagtagattt acaacaaaat gatgctcctg
    14641 aaacacaaaa agaagttact gattaatacg caatttacaa aacaggcaaa aagatactaa
    14701 gctgaatttt attgatgatt cagtttagta ctttaagcca ttttaaataa atgcaaatca
    14761 atcaaatagc acagctaatc taaattgaag gaggtaggct ccttgattga tgtaaataat
    14821 ttccattata tgaaaatagg attggcttca cctgaaaaaa tccgttcttg gtcttttggt
    14881 gaagttaaaa aacctgaaac aatcaactac cgtacattaa aacctgaaaa agatggtcta
    14941 ttctgtgaaa gaattttcgg acctacaaaa gactgggaat gtagttgtgg taaatacaaa
    15001 cgtgttcgct acaaaggcat ggtctgtgac agatgtggag ttgaagtaac taaatctaaa
    15061 gtacgtcgtg aaagaatggg tcacattgaa cttgctgctc cagtttctca catttggtat
    15121 ttcaaaggta taccaagccg tatgggatta ttacttgaca tgtcaccaag agcattagaa
    15181 gaagttattt actttgcttc ttatgttgtt gtagatccag gtccaactgg tttagaaaag
    15241 aaaactttat tatctgaagc tgaattcaga gattattatg ataaataccc aggtcaattc
    15301 gttgcaaaaa tgggtgcaga aggtattaaa gatttacttg aagagattga tcttgacgaa
    15361 gaacttaaat tgttacgcga tgagttggaa tcagctactg gtcaaagact tactcgtgca
    15421 attaaacgtt tagaagttgt tgaatcattc cgtaattcag gtaacaaacc ttcatggatg
    15481 attttagatg tacttccaat catcccacca gaaattcgtc caatggttca attagatggt
    15541 ggacgatttg caacaagtga cttaaacgac ttataccgtc gtgtaattaa tcgaaataat
    15601 cgtttgaaac gtttattaga tttaggtgca cctggtatca tcgttcaaaa cgaaaaacgt
    15661 atgttacaag aagccgttga cgctttaatt gataatggtc gtcgtggtcg tccagttact
    15721 ggcccaggta accgtccatt aaaatcttta tctcatatgt taaaaggtaa acaaggtcgt
    15781 ttccgtcaaa acttacttgg taaacgtgtt gactattcag gacgttcagt tattgcagta
    15841 ggtccaagct tgaaaatgta ccaatgtggt ttaccaaaag aaatggcact tgaactattt
    15901 aaaccattcg taatgaaaga attagttcaa cgtgaaattg caactaacat taaaaatgcg
    15961 aagagtaaaa tcgaacgtat ggatgatgaa gtttgggacg tattggaaga agtaattaga
    16021 gaacatcctg tattacttaa ccgtgcacca acacttcata gacttggtat tcaagcattt
    16081 gaaccaactt tagttgaagg tcgtgcgatt cgtctacatc cacttgtaac aacagcttat
    16141 aacgctgact ttgacggtga ccaaatggcg gttcacgttc ctttatcaaa agaggcacaa
    16201 gctgaagcaa gaatgttgat gttagcagca caaaacatct tgaaccctaa agatggtaaa
    16261 cctgtagtta caccatcaca agatatggta cttggtaact attaccttac tttagaaaga
    16321 aaagatgcag taaatacagg cgcaatcttt aataatacaa atgaagtatt aaaagcatat
    16381 gcaaatggct ttgtacattt acacactaga attggtgtac atgcaagttc attcaataat
    16441 ccaacattta ctgaagaaca aaacaaaaag attcttgcta cgtcagtagg taaaattata
    16501 ttcaatgaaa tcattccaga ttcatttgct tatattaatg aacctacgca agaaaactta
    16561 gaaagaaaga caccaaacag atatttcatc gatcctacaa ctttaggtga aggtggatta
    16621 aaagaatact ttgaaaatga agaattaatt gaacctttca acaaaaaatt cttaggtaat
    16681 attattgcag aagtattcaa cagatttagc atcactgata catcaatgat gttagaccgt
    16741 atgaaagact taggattcaa attctcatct aaagctggta ttacagtagg tgttgctgat
    16801 atcgtagtat tacctgataa gcaacaaata cttgatgagc atgaaaaatt agtcgacaga
    16861 attacaaaac aattcaaccg tggtttaatc actgaagaag aaagatataa tgcagttgtt
    16921 gaaatttgga cagatgcaaa agatcaaatt caaggtgaat tgatgcaatc acttgataaa
    16981 actaacccaa tcttcatgat gagtgattca ggtgcccgtg gtaacgcatc taactttaca
    17041 cagttagcag gtatgcgtgg attgatggcc gcaccatctg gtaagattat cgaattacca
    17101 atcacatctt cattccgtga aggtttaaca gtacttgaat acttcatctc aactcacggt
    17161 gcacgtaaag gtcttgccga tacagcactt aaaacagctg actcaggata tcttactcgt
    17221 cgtcttgttg acgtggcaca agatgttatt gttcgtgaag aagactgtgg tactgataga
    17281 ggtttattag tttctgatat taaagaaggt acagaaatga ttgaaccatt tatcgaacgt
    17341 attgaaggtc gttattctaa agaaacaatt cgtcatcctg aaactgatga aataatcatt
    17401 cgtcctgatg aattaattac acctgaaatt gctaagaaaa ttacagatgc tggtattgaa
    17461 caaatgtata ttcgctcagc atttacttgt aacgcacgac atggtgtttg tgaaaaatgt
    17521 tacggtaaaa accttgctac tggtgaaaaa gttgaagttg gtgaagcagt tggtacaatt
    17581 gcagcccaat ctatcggtga accaggtaca cagcttacaa tgcgtacatt ccatacaggt
    17641 ggggtagcag gtagcgatat cacacaaggt cttcctcgta ttcaagagat tttcgaagca
    17701 cgtaacccta aaggtcaagc ggtaattacg gaaatcgaag gtgtcgtaga agatattaaa
    17761 ttagcaaaag atagacaaca agaaattgtt gttaaaggtg ctaatgaaac aagatcatac
    17821 cttgcttcag gtacttcaag aattattgta gaaatcggtc aaccagttca acgtggtgaa
    17881 gtattaactg aaggttctat tgaacctaag aattacttat ctgttgctgg attaaacgcg
    17941 actgaaagct acttattaaa agaagtacaa aaagtttacc gtatgcaagg tgtagaaatc
    18001 gacgataaac acgttgaggt tatggttcga caaatgttac gtaaagttag aattatcgaa
    18061 gcaggtgata cgaagttatt accaggttca ttagttgata ttcataactt tacagatgca
    18121 aatagagaag catttaaaca ccgtaagcgt cctgcaacag ctaaaccagt attacttggt
    18181 attactaaag catcacttga aacagaaagt ttcttatctg cagcatcatt ccaagaaaca
    18241 acaagagttc ttacagatgc agcaattaaa ggtaagcgtg atgacttatt aggtcttaaa
    18301 gaaaacgtaa ttattggtaa gttaattcca gctggtactg gtatgagacg ttatagcgac
    18361 gtaaaatacg aaaaaacagc taaaccagtt gcagaagttg aatctcaaac tgaagtaacg
    18421 gaataacaag tatataacag aggctaatgc tttagcctct tgttattttt atgtaaatta
    18481 tttgatttaa tgttgacgaa ttctcttgtt caatgttaat atattaaagg ttgatgcaag
    18541 cagaactttg gaggataaat tattgtctaa ggaaaaagtt gcacgcttta acaaacaaca
    18601 ttttgtagtt ggtcttaaag aaacgcttaa agcgttaaag aaagatcaag ttacatcttt
    18661 gattattgct gaagacgttg aagtatattt aatgactcgc gtgttaagcc aaatcaatca
    18721 gaaaaatata cctgtatctt ttttcaaaag caaacatgct ttgggtaaac atgtaggtat
    18781 taacgtcaat gcgacaatag tagcattgat taaatgagaa ttagtaagtg ttttacttac
    18841 taaattttat ttaacctaaa aatgaaccac ctggatgtgt gggattaaaa agtgaagaga
    18901 ggaggacata tcacatgcca actattaacc aattagtacg taaaccaaga caaagcaaaa
    18961 tcaaaaaatc agattctcca gctttaaata aaggtttcaa cagtaaaaag aaaaaattta
    19021 ctgacttaaa ctcaccacaa aaacgtggtg tatgtactcg tgtaggtaca atgacaccta
    19081 aaaaacctaa ttcagcgtta cgtaaatatg cacgtgtgcg tttatcaaac aacatcgaaa
    19141 ttaacgcata catccctggt atcggccata acttacaaga acacagtgtt gtacttgtac
    19201 gtggtggacg tgtaaaagac ttaccaggtg tgcgttacca tattgtacgt ggagcacttg
    19261 atacttcagg tgttgacgga cgtagacaag gtcgttcatt atacggaact aagaaaccta
    19321 aaaactaaga atttagtttt taattaaatc ttaaacttaa aatatttaat ataaggaagg
    19381 gaggatttac attatgcctc gtaaaggatc agtacctaaa agagacgtat taccagatcc
    19441 aattcataac tctaagttag taactaaatt aattaacaaa attatgttag atggtaaacg
    19501 tggaacagca caaagaattc tttattcagc attcgaccta gttgaacaac gcagtggtcg
    19561 tgatgcatta gaagtattcg aagaagcaat caacaacatt atgccagtat tagaagttaa
    19621 agctcgtcgt gtaggtggtt ctaactatca agtaccagta gaagttcgtc cagagcgtcg
    19681 tactacttta ggtttacgtt ggttagttaa ctatgcacgt cttcgtggtg aaaaaacgat
    19741 ggaagatcgt ttagctaacg aaattttaga tgcagcaaat aatacaggtg gtgccgttaa
    19801 gaaacgtgag gacactcaca aaatggctga agcaaacaaa gcatttgctc actaccgttg
    19861 gtaagataaa agcttttacc ctgagtgtgt tctatattaa tgaattttca ttaagcgttc
    19921 atgcttaggg catcgccata tctatcgtat ttattcagta atataaactg gaaggagaaa
    19981 aaatacatgg ctagagaatt ttcattagaa aaaactcgta atatcggtat catggctcac
    20041 attgatgctg gtaaaacgac tacgactgaa cgtattcttt attacactgg ccgtatccac
    20101 aaaattggtg aaacacacga aggtgcttca caaatggact ggatggagca agaacaagac
    20161 cgtggtatta ctatcacatc tgctgcaaca acagcagctt gggaaggtca ccgtgtaaac
    20221 attatcgata cacctggaca cgtagacttc actgtagaag ttgaacgttc attacgtgta
    20281 cttgacggag cagttacagt acttgatgca caatcaggtg ttgaacctca aactgaaaca
    20341 gtttggcgtc aggctacaac ttatggtgtt ccacgtatcg tatttgtaaa caaaatggac
    20401 aaattaggtg ctaacttcga atactctgta agtacattac atgatcgttt acaagctaac
    20461 gctgctccaa tccaattacc aattggtgcg gaagacgaat tcgaagcaat cattgactta
    20521 gttgaaatga aatgtttcaa atatacaaat gatttaggta ctgaaattga agaaattgaa
    20581 attcctgaag accacttaga tagagctgaa gaagctcgtg ctagcttaat cgaagcagtt
    20641 gcagaaacta gcgacgaatt aatggaaaaa tatcttggtg acgaagaaat ttcagtttct
    20701 gaattaaaag aagctatccg ccaagctact actaacgtag aattctaccc agtactttgt
    20761 ggtacagctt tcaaaaacaa aggtgttcaa ttaatgcttg acgctgtaat tgattactta
    20821 ccttcaccac tagacgttaa accaattatt ggtcaccgtg ctagcaaccc tgaagaagaa
    20881 gtaatcgcga aagcagacga ttcagctgaa ttcgctgcat tagcgttcaa agttatgact
    20941 gacccttatg ttggtaaatt gacattcttc cgtgtgtatt caggtacaat gacatctggt
    21001 tcatacgtta agaactctac taaaggtaaa cgtgaacgtg taggtcgttt attacaaatg
    21061 cacgctaact cacgtcaaga aatcgatact gtatactctg gagatatcgc tgctgcggta
    21121 ggtcttaaag atacaggtac tggtgatact ttatgtggtg agaaaaatga cattatcttg
    21181 gaatcaatgg aattcccaga gccagttatt cacttatcag tagagccaaa atctaaagct
    21241 gaccaagata aaatgactca agctttagtt aaattacaag aagaagaccc aacattccat
    21301 gcacacactg acgaagaaac tggacaagtt atcatcggtg gtatgggtga gcttcactta
    21361 gacatcttag tagaccgtat gaagaaagaa ttcaacgttg aatgtaacgt aggtgctcca
    21421 atggtttcat atcgtgaaac attcaaatca tctgcacaag ttcaaggtaa attctctcgt
    21481 caatctggtg gtcgtggtca atacggtgat gttcacattg aattcacacc aaacgaaaca
    21541 ggcgcaggtt tcgaattcga aaacgctatc gttggtggtg tagttcctcg tgaatacatt
    21601 ccatcagtag aagctggtct taaagatgct atggaaaatg gtgtcttagc aggttatcct
    21661 ttaattgatg ttaaagctaa attatatgat ggttcatacc atgatgtcga ttcatctgaa
    21721 atggccttca aaattgctgc atcattagca cttaaagaag ctgctaaaaa atgtgatcct
    21781 gtaatcttag aaccaatgat gaaagtaact attgaaatgc ctgaagagta catgggtgat
    21841 atcatgggtg acgtaacatc tcgtcgtgga cgtgttgatg gtatggaacc tcgtggtaat
    21901 gcacaagttg ttaatgctta tgtaccactt tcagaaatgt tcggttatgc aacatcatta
    21961 cgttcaaaca ctcaaggtcg cggtacttac actatgtact tcgatcacta tgctgaagtt
    22021 ccaaaatcaa tcgctgaaga tattatcaag aaaaataaag gtgaataata taacttgttt
    22081 tgactagcta gcctaggtta aaatacaagg tgagcttaaa tgtaagctat catctttata
    22141 gtttgatttt ttggggtgaa tgcattataa aagaattgta aaattctttt tgcatcgcta
    22201 taaataattt ctcatgatgg tgagaaacta tcatgagaga taaatttaaa tattattttt
    22261 aattagaata ggagagattt tataatggca aaagaaaaat tcgatcgttc taaagaacat
    22321 gccaatatcg gtactatcgg tcacgttgac catggtaaaa caacattaac agcagcaatc
    22381 gctactgtat tagcaaaaaa tggtgactca gttgcacaat catatgacat gattgacaac
    22441 gctccagaag aaaaagaacg tggtatcaca atcaatactt ctcacattga gtaccaaact
    22501 gacaaacgtc actacgctca cgttgactgc ccaggacacg ctgactacgt taaaaacatg
    22561 atcactggtg ctgctcaaat ggacggcggt atcttagtag tatctgctgc tgacggtcca
    22621 atgccacaaa ctcgtgaaca cattctttta tcacgtaacg ttggtgtacc agcattagta
    22681 gtattcttaa acaaagttga catggttgac gatgaagaat tattagaatt agtagaaatg
    22741 gaagttcgtg acttattaag cgaatatgac ttcccaggtg acgatgtacc tgtaatcgct
    22801 ggttcagcat taaaagcttt agaaggcgat gctcaatacg aagaaaaaat cttagaatta
    22861 atggaagctg tagatactta cattccaact ccagaacgtg attctgacaa accattcatg
    22921 atgccagttg aggacgtatt ctcaatcact ggtcgtggta ctgttgctac aggccgtgtt
    22981 gaacgtggtc aaatcaaagt tggtgaagaa gttgaaatca tcggtttaca tgacacatct
    23041 aaaacaactg ttacaggtgt tgaaatgttc cgtaaattat tagactacgc tgaagctggt
    23101 gacaacattg gtgcattatt acgtggtgtt gctcgtgaag acgtacaacg tggtcaagta
    23161 ttagctgctc ctggttcaat tacaccacat actgaattca aagcagaagt atacgtatta
    23221 tcaaaagacg aaggtggacg tcacactcca ttcttctcaa actatcgtcc acaattctat
    23281 ttccgtacta ctgacgtaac tggtgttgtt cacttaccag aaggtactga aatggtaatg
    23341 cctggtgata acgttgaaat gacagtagaa ttaatcgctc caatcgcgat tgaagacggt
    23401 actcgtttct caatccgtga aggtggacgt actgtaggat caggcgttgt tactgaaatc
    23461 attaaataat ttctaatttc ttagatttta tataaaaaga agatccctca atcgaggggt
    23521 ctttttttaa tgtgtaaatt ttgtaatggc tattcgattt agaagaacaa taattgatga
    23581 aagactgact aataaaactt ataactgata atactgttta aataaaattg ttgagtcttg
    23641 gacattgtaa aatgctccct tcaaagtttt cattttttca atgtctactt tgaagggagc
    23701 atttcattag tttatgtctc agattcatat ctttcaatta atttaaatgc ttaatttgtt
    23761 ttaaatactt gctctaattc aatgattttt aaaaatacag ctacagcgta ttttaatgat
    23821 ttttcatcaa tatcaaattt gggattatgg tgtggcgctg taataccttt actttcatta
    23881 ccacaaccag tcagaaagaa tgcacctggt cgtactttca aataatgtga aaaatcttct
    23941 ccaatcatca ttaaatctga ttcattaaag cgtacatgta agtcatttgt tgcttcttta
    24001 ataacttgat atgctttctc gttattatgg acaggcaaat accctttaat ataattcaaa
    24061 tcatagttaa tatcatttgc tattgctaaa ccttgtaaaa gcttatccat tttgtccatt
    24121 acatgattct gtatatctga atcgaaagtt ctaactgtac ctttacaaaa tgcttgatca
    24181 ggaataacgc tatctgtggt gcctgcttga atcattccaa atgaaagtac agcttgttta
    24241 actggatcga tcgtacgtga aattattttt tgtgcactta aaatgaactc tgccatgatt
    24301 actattgggt caatggtttc atgaggtttg gcaccatgac cgccacgacc tttaattgtg
    24361 acactaaatt catctggaga ggccatgatt gcccccgcac gtgaatgaat agttccagta
    24421 ggataaccac tccataaatg tgtaccgtaa attctatcta cattttccag acatccagca
    24481 tctatcattt cttgagaacc acctggcatg atttcttcac cgtactggaa tattaataca
    24541 acattacctt ctaataaatg tttatgttca tctaaaatct ctgctacagt aagtaaaatt
    24601 gctgtatgac catcatgccc acacgcatgc atacatcctg gatttttaga cttataaggc
    24661 acatcgttta attcctcgac aggtaacgca tcaaagtcag ctcttaatgc aatggtaggt
    24721 cctgtgccca agcctttaaa tgtggctttg ataccattgc ggccgatagg agtttcaata
    24781 tcacaagata actggcttaa ttggttaaca atataatcat gtgtttgaaa ttcttcaaaa
    24841 gataactcag gatattggtg taaataacgt ctgagttgaa ttgttttatt ttctttatta
    24901 tttgctagtt ggaaccaatc taacaccctt atcactactt tctaaaataa tgtttatagt
    24961 ataacatttt atgaaattat cgtactaaat gattgctttg agatatttta tctatgaatg
    25021 ataaggcttt caagttatgt agaattactg tatgataaag gtattaccaa acaatactta
    25081 agggggatta tatactgtgg ttcaatcatt acatgagttt ttagaggaaa atataaatta
    25141 tctaaaagaa aatggtttgt ataatgaaat agatacaatt gaaggtgcaa acggaccaga
    25201 aatcaaaatc aatgggaaat catacattaa cttatcttca aataattatt taggactagc
    25261 aacaaatgaa gatttgaaat cagctgcaaa agcagctatt gatacacatg gtgtaggtgc
    25321 aggcgctgtt cgtacaatca atggtacatt agatttacac gacgaattag aagaaacact
    25381 agcaaaattt aaaggaacag aagctgcaat agcttatcaa tcaggattta attgtaatat
    25441 ggctgctatt tcggctgtga tgaataaaaa tgatgctatt ttatcagatg agcttaatca
    25501 tgcatcaatt attgatggat gtcgcttatc taaagctaaa attattcgag ttaaccattc
    25561 agacatggat gatttacgtg cgaaagcaaa agaagcagtt gaatcaggtc aatacaataa
    25621 agtgatgtat atcactgatg gcgtttttag tatggatggt gatgtggcta aattacctga
    25681 aattgtagaa attgcagaag aatttggttt attaacttat gttgacgacg ctcatggttc
    25741 aggtgttatg ggtaaaggcg ctggtacggt taaacatttt ggtttacaag ataaaatcga
    25801 tttccaaata ggtacgcttt ctaaagcaat tggtgtcgtt ggcggttatg tagcaggtac
    25861 aaaagagtta atagattggt taaaagcaca atcacgacca ttcttattct ctacatcatt
    25921 agcacctggg gataccaaag caataactga agcagttaaa aagttaatgg attcaactga
    25981 attacatgat aaattatggg acaatgcaca atatttaaaa aatggattgt caaaattagg
    26041 atatgataca ggtgagtcag aaactccaat tacaccagta attattggtg atgaaaaaac
    26101 aactcaagaa tttagtaagc gtttaaaaga cgaaggtgtc tatgtgaaat ctatcgtttt
    26161 cccaacagta ccaagaggta caggacgtgt aagaaatatg cctacagctg cacatacaaa
    26221 agacatgtta gatgaagcaa ttgcggctta tgaaaaagta ggaaaagaaa tgaagttgat
    26281 ttaatattta tttattccca cggcaaatat tgtcgtgggc tttttttaat gtttagttta
    26341 ttaacagtaa gttcttataa caatgtttag tgctccccaa aaattaaagt ttgaatttta
    26401 aaagcatctt gtagaattta gttgtatttt ttacaaagaa attcattttg attatttttg
    26461 ataatgagca ttttaatagt aatacatgtt tatagtgtgt agtatatgtc tatactagta
    26521 gtaactatat agagaaagta ggaataaact atgtcacaag atgtaaatga attaagtaag
    26581 caaccaacgc cagataaagc agaagataac gcatttttcc catcaccata ttcccttagt
    26641 caatatacag caactaaaac agattttgat ggtgttgaac acaaaggtgc ctataaagat
    26701 ggtaaatgga aagtattgat gattgctgct gaagagagat atgtattatt ggaaaatgga
    26761 aaaatgttct ctacgggtaa tcatcctgtt gaaatgttat tacctttaca tcatttaatg
    26821 gaagcaggtt ttgacgttga tgttgcgaca ttatctggtt atccagttaa attagaatta
    26881 tgggctatgc caactgaaga cgaggcagtt attagtactt ataataaatt gaaagaaaaa
    26941 ttaaaacagc caaaaaaatt agcagatgtg attaaaaatg aattaggacc tgattcagac
    27001 tatttatctg tctttatccc aggcggacat gctgcagttg ttggtatttc tgaaagtgag
    27061 gacgttcaac aaacattaga ttgggcatta gacaatgacc gctttatagt tacattatgt
    27121 catggaccag cagcactact ttcagcaggg ctcaacagag aaaaatctcc attagaagga
    27181 tattctgttt gtgtcttccc tgactcatta gatgaaggtg caaatattga aataggttat
    27241 ttacctggac gcttgaaatg gttagttgct gatttactaa ctaaacaagg attaaaagta
    27301 gttaacgacg atatgacagg cagaacgtta aaagatcgta aattattaac aggtgacagt
    27361 cctttagctt caaatgagtt aggaaaatta gcagttaatg aaatgttaaa tgcaatacaa
    27421 aataaataat taaatattaa ttagaggagc ctcatatgta aatgtatgag ggctcttttt
    27481 ttggcaaaat ttaagtgata cttgtaaaat agaacctatt atgagtatga tttaagaaaa
    27541 cgcttgcaaa actaataacc gcaactagcg atatggagga aacatgatgt cttatagcat
    27601 tggaattgat tatggaacag cttcaggccg tgtgttttta attaatacaa ctaacggtca
    27661 agtagtatca aaatttgtga aaccatatac acatggtgtc attgagagtg aattaaatgg
    27721 tttgaaaata ccatatacat atgcacttca aaatagtaat gattatttag aaattatgga
    27781 agaaggaata tcatatatag tacgtgaatc aaaaatagat ccagtcaata tagtaggtat
    27841 tggtatagac tttacttcat ctactattat ttttactgac gaaaacctta acccggtaca
    27901 taatttaaaa caatttaaaa acaatccaca tgcgtatgtg aaactttgga aacatcatgg
    27961 tgcatataaa gaggcagaga aattatatca aactgctatt gaaaataata ataagtggtt
    28021 aggccattat ggatataatg ttagtagtga atggatgatt cccaaaataa tggaggtcat
    28081 gaatcgagca ccagaaatta tggaaaaaac ggcttatatt atggaagcgg gcgattggat
    28141 tgtaaataaa ttaactaata aaaatgtacg ctcgaattgt ggattaggtt tcaaagcatt
    28201 ttgggaagaa gaaacagggt ttcattatga tttatttgat aaaatagacc ccaaattatc
    28261 aaaagtaatt caagataaag tatctgcacc ggttgttaat attggtgaag tagtagggaa
    28321 gctggacgat aaaatggcac agaaattagg attatcaaaa gagactatgg taagtccttt
    28381 tattattgat gcccatgcta gtttattagg tattgggtct gaaaaagata aagaaatgac
    28441 tatggtgatg ggaacaagca catgccatct tatgttaaat gaaaagcaac atcaagtgcc
    28501 aggtatatca ggttctgtaa aaggagcaat tattccagaa ttatttgctt atgaagcggg
    28561 acaatcagca gtaggtgatt tgtttgagta tgtcgctaag caagcaccaa agtcatatgt
    28621 agatgaagca gcaaatagaa atatgactgt atttgaatta atgaatgaaa agataaaaca
    28681 tcaaatgcca ggtgaaagcg ggctcattgc tcttgattgg cataatggaa atcgaagtgt
    28741 attaagtgat agcaatttaa caggttgtat ctttggatta actttacaaa ctaagcatga
    28801 ggatatttat agagcatatt tagaagctac agcatttggt actaagatga ttatgcaaca
    28861 gtatcaagat tggcatatgg aagtagaaaa ggtatttgca tgtggcggta tacctaaaaa
    28921 gaatgctgtt atgatggata tctatgcgaa tgtactgaat aaaaaattaa ttgttatgga
    28981 tagtgagtat gcaccagcaa taggcgcagc aatattaggt gcagtcagtg gtggcgcaca
    29041 taattcaatt aatgatgcag ttgatgctat gaaagagcca attttatacg aaattaatcc
    29101 agaagcggaa aaagtacaaa ggtatgaaac attatttaaa gcttataagg ctttacatga
    29161 tatccatggt tataaaaaag ctaatataat gaaagatatc cagagtttaa gagttgaggg
    29221 ataaaaaatt taatttgaca gtaattagca ataataaacg ctataaatta ctaaaatcaa
    29281 ttaatacaac tagaatttcc atttacagaa atatcagcaa catgtacatg tcatatatac
    29341 aagaaagcgc ttttatatta taattattat gaaaatgaat attatgtccg cttgtgatgg
    29401 actatagcta tatagaagag acaaaggaga tatttctatg aaaaaaatta tgattactgg
    29461 tgcattagga caaattggta cagaattagt tgttaagtgc agagaaattt atgggacaga
    29521 taatgttctt gctacagata ttagggaacc tgaagcagac tcacctgtac aaaatggacc
    29581 atttgaaatc ttagacgtaa cagatcgtga ccgtatgttt gagttagtta gggactttga
    29641 agcggatagt ctaatgcata tggcagcatt attatcagca actgctgaga aaaatccaat
    29701 tctagcttgg gatttaaata tgggtggatt aatgaatgca ttagaagctg caagaactta
    29761 taatttgcac tttttcacac caagttcaat tggtgcattt ggagactcaa ctcctaaagt
    29821 taatacgcca caagtaacga ttcagcaacc tacgacaatg tatggtgtaa ataaagtagc
    29881 tggagaatta ttgtgtcaat actatttcaa acgttttggt gtagatacaa gaagtgttag
    29941 attcccaggt ttaatctcgc atgttaaaga gccaggtggc ggtactacag actatgctgt
    30001 tgaaatatac ttcaaagcag taagagaggg tcattataca agcttcatag ataaaggcac
    30061 gtatatggat atgatgtata tggatgatgc aattgaagca attattaaac ttatggaagc
    30121 agacgacgct aaattagaaa ctagaaacgg ttataatttg agcgcaatga gttttgatcc
    30181 agagatggta aaagaagcaa ttcaagaata ctatccaaat tttacattag attacgatgt
    30241 tgatcctatt agacaaggta tcgctaatag ttggccggat tctattgata caagctgttc
    30301 acgtggcgaa tggggatttg atcctaaata tgatttagcg agcatgacta aattaatgtt
    30361 agaagctatt gaacaaaaag atactgttaa aaataataac taatcatttc cattcacttt
    30421 aatacacgga atgatatttt aaattactct ttattttaat aaactagtgc atgaattcta
    30481 atattattca ttatacttat tgaattcgcg agcttagttc attttaaagt aaagagtttt
    30541 ttgtatgtaa agtatattag taaaacacag attacttgtc tctgtgaaat taatacg
//
LOCUS       DAHHOJ010000022        30226 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.022, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000022 DAHHOJ010000000
VERSION     DAHHOJ010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 30226)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30226)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30226
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.022"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            162..614
                     /gene="argR"
                     /locus_tag="NBH32_000830"
     CDS             162..614
                     /gene="argR"
                     /locus_tag="NBH32_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCD1514454.1"
                     /translation="MPKKSVRHIKIREIISNEQIETQDELVKRLNDYDLNVTQATVSR
                     DIKELQLIKVPIPSGQYVYSLPNDRKFHPLEKLGRYLMDSFVNIDGTDNLLVLKTLPG
                     NAQSIGAILDQINWEEVLGTICGDDTCLIICRSKEASDEIKSRIFNLL"
     gene            630..2309
                     /gene="recN"
                     /locus_tag="NBH32_000831"
     CDS             630..2309
                     /gene="recN"
                     /locus_tag="NBH32_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCD1514455.1"
                     /translation="MLQTLSIKQFAIIEELEIQFSDGLTVLSGETGSGKSIIIDAIGQ
                     LIGMRASSDFVRHGEKKAVIEGIFDIDESKDAIHILKNMDIDVDEDFLLVKREIFSSG
                     KSLCKINNQTVTLQDLRKVMQELLDIHGQHETQSLLKQKYHLTLLDNYAESRYQDLLD
                     KYHQTFQNYKAKKQELEDLESADQALLQRLDLMKFQLEELSEAHLKEGEIEQLEIDIK
                     RIQNSEKLSLALNNAHMTLTDENAITDRLYELSNHLLTINDIVPNKYDKLKEDIDQFY
                     YILEDAKHELYDEMANTEFDEQVLNEYESRMNLLNNLKRKYGKDISELISYQEKLNNE
                     INKIENYEQSTSQLREEINALYNQVIEVGQALSKQRRIVARELRDHIVSEIQNLQMKD
                     ANLEISFKKLEEPNIDGIEFVEFLISPNKGEPLKSLNKIASGGELSRIMLALKSIFVK
                     SRGQTAILFDEVDSGVSGQAAQKMAEKMRDIAEYIQVICISHLPQVASMSDHHLLISK
                     SSKDDRTTTQVQELIGDDKVDEIARMISGASVTDLTRENAREMIQHNQRRR"
     gene            2460..3881
                     /gene="lpdA"
                     /locus_tag="NBH32_000832"
     CDS             2460..3881
                     /gene="lpdA"
                     /locus_tag="NBH32_000832"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1514456.1"
                     /translation="MSEKQYDLVVLGGGTAGYVAAIRASQLGKKVAIVERQLLGGTCL
                     HKGCIPTKSLLKSAEVFQTVKQAAMFGVDVKDANVNFENMLARKEDIINQMYQGVKHL
                     MQHNHIDIYNGTGRILGTSIFSPQSGTISVEYEDGESDLLPNQFVLIATGSSPAELPF
                     LSFDHDKILSSDDILSLKTLPSSIGIIGGGVIGMEFASLMIDLGVDVTVIEAGERILP
                     TESKQASQLLKKSLSARGVKFYEGIKLSENDINVNEDGVTFEISSDIIKVDKVLLSIG
                     RKPNTSDIGLNNTKIKLSISGHILTNEFQQTEDKHIYAAGDCIGKLQLAHVGSKEGVV
                     AVDHMFEGNPIPVNYNMMPKCIYSQPEIASIGLNIEQAKAEGMKVKSFKVPFKAIGKA
                     VIDSHDANEGYSEMVIDQSTEEIVGINMIGPHVTELINEASLLQFMNGSALELGLTTH
                     AHPSISEVLMELGLKAESRAIHV"
     gene            3897..4889
                     /locus_tag="NBH32_000833"
     CDS             3897..4889
                     /locus_tag="NBH32_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HCD1514457.1"
                     /translation="MIDYKSLGLSEEDLKVIYKWMDLGRKIDERLWLLNRAGKIPFVV
                     SGQGQEATQIGMAYALEEGDITAPYYRDLAFVTYMGISAYDTFLSAFGKKDDVNSGGK
                     QMPSHFSSRAKNILSQSSPVATQIPHAVGAALAIKMDGKKKIATATVGEGSSNQGDFH
                     EGLNFAGVHKLPFVCVIINNKYAISVPDSLQYAAEKLSDRALGYGIHGEQVDGNDPLA
                     MYKAMKEARDRAISGQGSTLIEAVTSRMTAHSSDDDDQYRTKEEREALKKADCNEKFK
                     KELLSAGIIDDAWLAEIEAEHKDIINKATKAAEDAPYPSVEEAYAFVYEEGSLN"
     gene            4889..5872
                     /locus_tag="NBH32_000834"
     CDS             4889..5872
                     /locus_tag="NBH32_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD1514458.1"
                     /translation="MAKLSYLEAIRQAQDLALQQNKDVFILGEDVGRKGGVFGTTQGL
                     QQKYGEDRVIDTPLAESNIVGTAIGAAMVGKRPIAEIQFADFILPATNQIISEAAKMR
                     YRSNNDWQCPLTIRAPFGGGVHGGLYHSQSIESIFASSPGLTIVIPSTPYDAKGLLLS
                     SIESNDPVLYFEHKKAYRFLKEEVPEEYYTVPLGKADVKREGEDLTVFCYGLMVNYCL
                     QAADILAADGINVEVVDLRTVYPLDKETIIDRAKHTGKVLLVTEDNLEGSIMSEVSAI
                     IAEHCLFDLDAPIMRLAAPDVPSMPFSPVLENEIMMNPEKILNKMRELAEF"
     gene            5885..7159
                     /locus_tag="NBH32_000835"
     CDS             5885..7159
                     /locus_tag="NBH32_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD1514459.1"
                     /translation="MEITMPKLGESVHEGTIEQWLVSVGDHIDEYEPLCEVITDKVTA
                     EVPSTISGTITEILVEAGQTVAIDTIICKIETADEKTNETTEEIQANVDEHTQKSTKK
                     ASATVEQTSTAKQNQPRNNGRFSPVVFKLASEHDIDLSQVVGSGFEGRVTKKDIMSVI
                     ENGGTTAQSDKQVQTQSTSVDTSSNQSSEDNSENSTIPVNGVRKAIAQNMVNSVTEIP
                     HAWMMIEVDATNLVNTRNHYKNSFKNKEGYNLTFFAFFVKAVADALKAYPLLNSSWQG
                     NEIVLHKDINISIAVADENKLYVPVIKHADEKSIKGIAREINTLATKARNKQLTAEDM
                     QGGTFTVNNTGTFGSVSSMGIINHPQAAILQVESIVKKPVVINDMIAIRNMVNLCISI
                     DHRILDGLQTGKFMNHIKQRIEQYTLENTNIY"
     gene            7510..7947
                     /locus_tag="NBH32_000836"
     CDS             7510..7947
                     /locus_tag="NBH32_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrxA/BrxB family bacilliredoxin"
                     /protein_id="HCD1514460.1"
                     /translation="MDMNFDLYMNGVVEQARNEIESAGYEQLTTAEDVDKVLKQDGTT
                     LVMINSVCGCAGGIARPAASHALHYDVLPDRLVTVFAGQDKEATQRAREYFEGYAPSS
                     PSFALVKDGKITEMIERHQIEGHDVMNVINQLQTLFNKYCEER"
     gene            7961..8941
                     /locus_tag="NBH32_000837"
     CDS             7961..8941
                     /locus_tag="NBH32_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1514461.1"
                     /translation="MLKLNPYKIGFRTIKTAVGMTLGVIISKLLGLDNYASSAILVVL
                     CIKHTKVHSLQAIISRLVSCFLVLFLGSAIFSLLGQSPIVLGIIVLLFIPLTVVLKVQ
                     EGVITSCVILLHVFNAKSIDVHLIVNETLLLLIGLSIAFTMNLMMPSLDKQLDEYKCK
                     IEQQIADIFSKYSYICEKYEDTIAIEFEALLLNIKKAKSIAFRDVKNHFVRNENSYYH
                     YFDMREEQVELLMRMKPLIESICHKDPLLNDVASLLKEIGLNVNSNDYTAMRLHNLYE
                     TRLKLDELPLPTTHKALNSRASIIQILNELEEYLQIKSQFGSLKMHSEVA"
     gene            complement(9068..9247)
                     /gene="prli42"
                     /locus_tag="NBH32_000838"
     CDS             complement(9068..9247)
                     /gene="prli42"
                     /locus_tag="NBH32_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stressosome-associated protein Prli42"
                     /protein_id="HCD1514462.1"
                     /translation="MLVISSQVKSFISYVVIINTKVIHLEAHKMQNKVLRIIIIVMLV
                     SVVLALLLTSIIPIL"
     gene            9510..10643
                     /locus_tag="NBH32_000839"
     CDS             9510..10643
                     /locus_tag="NBH32_000839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HCD1514463.1"
                     /translation="MINEQRLLNTFLELVQIDSETGNESTIQPILKEKFIALGLDVKE
                     DEAAKHPKLGANNLVCTMNSTIEEGEVPKLYLTSHMDTVVPAINVKAIVKEDGYIYSD
                     GTTILGADDKAGLAAMLEVLQVIKEQQIPHGQIQFVITVGEESGLIGAKELNSELLDA
                     DFGYAIDASADVGTTVVGAPTQMLISAKIIGKTAHASTPKEGVSAINIAAKAISRMKL
                     GQVDEITTANIGKFHGGSATNIVADEVILEAEARSHDPERIKTQVKHMTDVFETTASE
                     LGGKAEVTVEQSYPGFKINDNEAVVKIAQESARNLGLSANTIISGGGSDGSIINTFGI
                     PSVILGVGYEKIHTTNERMPIKSLNLLASQVLEIIKIVARQSK"
     gene            10711..12117
                     /gene="gndA"
                     /locus_tag="NBH32_000840"
     CDS             10711..12117
                     /gene="gndA"
                     /locus_tag="NBH32_000840"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCD1514464.1"
                     /translation="MTQQIGVIGLAVMGKNLAWNIESRGYSVSVFNRSSEKTDLMVEE
                     SKGKNIHPTYSLEEFVNSLEKPRKILLMVQAGKATDATIDSLLPLLDDGDILIDGGNT
                     NYQDTIRRNKALAQSAINFIGMGVSGGEIGALTGPSLMPGGQEEAYNKVADILDAIAA
                     KAKDGASCVTYIGPNGAGHYVKMVHNGIEYADMQLIAESYAMMKELLGMSHEDIAQTF
                     KDWNAGELESYLIEITGDIFMKLDENKEALVEKILDTAGQKGTGKWTSINALELGIPL
                     TIITESVFARFISSIKEERVNASKELNGPKASFDGDKKDFLEKIRKALYMSKICSYAQ
                     GFAQMRKASEDNEWNLKLGDLAMIWREGCIIRAQFLQKIKDAYDNNPGLQNLLLDPYF
                     KNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAH
                     TYERKDKEGVFHTQWIEE"
     gene            12336..12707
                     /locus_tag="NBH32_000841"
     CDS             12336..12707
                     /locus_tag="NBH32_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1514465.1"
                     /translation="MEKLNQVMLYVDDVDKAKAFWTETLEFVVVSETPLAEDYVAVEV
                     SPTKDAETSLTIMAKEFIEKYSPEVNLGTPSLMFKEKNFDALYSKLNDLGLTGHDIVE
                     MNGQRAFNFQDGQGNYFAVSD"
     gene            12646..12882
                     /locus_tag="NBH32_000842"
     CDS             12646..12882
                     /locus_tag="NBH32_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514466.1"
                     /translation="MVNVHLISKMAKVTILQLVTKQSILIFIQTFILIFGLNFSMKVL
                     FLSFYAIVCTNLLTFLESVTKLVLYNGWKRFPAQ"
     gene            12901..13920
                     /locus_tag="NBH32_000843"
     CDS             12901..13920
                     /locus_tag="NBH32_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1514467.1"
                     /translation="MVTIKDVALKAGVSPSTVSRVIKGNQRISEATISKVKKVMEELN
                     YFPNTAARTLITNQTYKIGLVLKGSEEPIRLNPFYINVLLGISETCNQHGYGTQTTVS
                     NNLNDLMDEVYKMIKQRMVDAFILLYSKENDPIKQMLIDESMPFIVIGKPTSDIDHQF
                     THIDNDNILASENLTRHVIEQGVDELIFITEKGNFEVSKDRIKGFETIASQNKINYQI
                     IETSNEREVIFNYMQNLHTRLKDPNIKQAIISLDAVLHLAILSVLYELNIEIPKDVMT
                     ATFNDSYLTEIASPPQTSIDIEPRMLGQQAGAAILNILKNKAQDVIELVIIDTELKIR
                     KSTQR"
     gene            13936..15585
                     /locus_tag="NBH32_000844"
     CDS             13936..15585
                     /locus_tag="NBH32_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCD1514468.1"
                     /translation="MNKQWWKEAVAYQVYPRSFNDSNHDGIGDLPGMIDKLDYLKDLG
                     IDVIWLSPMFKSPNDDNGYDISDYQEIMDEFGTMEDFDRLLKGVHDRGMKLILDLVVN
                     HTSDEHPWFIESKSSKDNPKRDWYIWQDPKPDGSEPNNWESIFNGSTWEYDANTEQYY
                     FHLFSKKQPDLNWANPEVRDAVFEMMNWWFDKGIDGFRVDAITHIKKTFEAGDLPVPE
                     DKTYAPAFDVDMNQPGIQTWLQEMKDRSLSKYDIMTVGEANGVSPDDADDWVGEENGK
                     FNMIFQFEHLGLWNSGDSHFDVNSYKSVLNRWQKQLENKGWNALFIENHDQPRRVSTW
                     GDDDKYWYESATSHAAVYFLQQGTPFIYQGQEIGMTNYPFESIETFNDVAVKNDYQIV
                     KAQGGDVDALLAKYKDENRDNSRTPMQWDDTLNGGFTNGEPWFPVNPNYKTINVAQQL
                     EDEHSVLQFYKDLIQLRKSNDVYVYGQFDLVDAENSQVFAYTRTLNEKQVLIVGNLTN
                     HEAELTVPFDLSHGEVKLFNYDAKVNLKQLRPYEACVIELN"
     gene            complement(15667..16533)
                     /locus_tag="NBH32_000845"
     CDS             complement(15667..16533)
                     /locus_tag="NBH32_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCD1514469.1"
                     /translation="MDVIKQIQQAIVYIEDRLLEPFNLQELSDYVGLSPYHLDQSFKM
                     IVGLSPEAYARARKMTLAANDVINGATRLVDIAKKYHYANSNDFANDFSDFHGVSPIQ
                     ASTKKDELQIQERLYIKLSTTERAPYPYRLEETDDISLVGYARFIDTKYLSHPFNVPD
                     FLEDLLIDGKIKELRRYNDVSPFELFVISCPLENGLEIFVGVPSERYPAHLESRFLPG
                     KHCAKFNLQGEIDYATNEAWYYIESSLQLTLPYERNDLYVEVYPLDISFNDPFTKIQL
                     WIPVKQSPYDED"
     gene            complement(16763..18247)
                     /locus_tag="NBH32_000846"
     CDS             complement(16763..18247)
                     /locus_tag="NBH32_000846"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCD1514470.1"
                     /translation="MSTKNKHIPCLITIFGATGDLSHRKLFPSIFHLYQQDNLDEHIA
                     IIGIGRRDITNDDFRNQVKSSIQKHVKDTNKIDAFMEHVFYHRHDVSNEESYQELLDF
                     SNELDSQFELKGNRLFYLAMAPQFFGVISDYLKSSGLTDTKGFKRLVIEKPFGSDLKS
                     AEALNNQIRKSFKEEEIYRIDHYLGKDMVQNIEVLRFANAMFEPLWNNKYISNIQVTS
                     SEILGVEDRGGYYESSGALKDMVQNHMLQMVALLAMEAPISLNSEDIRAEKVKVLKSL
                     RHFQSEDVKKNFVRGQYGEGYIDGKQVKAYRDEDRVADDSNTPTFVSGKLTIDNFRWA
                     GVPFYIRTGKRMKSKTIQVVVEFKEVPMNLYYETDKLLDSNLLVINIQPNEGVSLHLN
                     AKKNTQGIETEPVQLSYSMSAQDKMNTVDAYENLLFDCLKGDATNFTHWEELKSTWKF
                     VDAIQDEWNMVDPEFPNYESGTNGPLESDLLLARDGNHWWDDIQ"
     gene            18560..19480
                     /gene="rnz"
                     /locus_tag="NBH32_000847"
     CDS             18560..19480
                     /gene="rnz"
                     /locus_tag="NBH32_000847"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HCD1514471.1"
                     /translation="MEVTFFGTSAGLPTKERNTQAIALNLEPYSNSIWLFDVGEGTQH
                     QILHHAIKLGKVTHIFITHMHGDHIFGLPGLLSSRSFQGGEQKPLTLVGPKGIKAYVE
                     MSMNLSESHLNYPITYIEIDDHLTYHHDGFTVEAHLLNHGIPSYGYRVMAPETTGTIN
                     VEALKNIGLEPGPKYQEVKSHDTFEHNGQVYQSKDFRGESKQGPVVAIFGDTKPCSNE
                     RVISRDADVMVHEATYIDGEKHLANNYHHSHIEDVFALIKEANVKRTLITHLSNRYNT
                     EDINEIYQTLIQNEDTPNFNFVKDFDSFKV"
     gene            complement(19583..20398)
                     /gene="proC"
                     /locus_tag="NBH32_000848"
     CDS             complement(19583..20398)
                     /gene="proC"
                     /locus_tag="NBH32_000848"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCD1514472.1"
                     /translation="MKLVFYGAGNMAQAIFTGIINSSNLDANDIYLTNKSNEQALKAF
                     AEKLGVNYSYDDATLLKDADYVFLGTKPHDFDALATRIKPHITKDNCFISIMAGIPID
                     YIKQQLECQNPVARIMPNTNAQVGHSVTGISFSNNFDPKSKDEINDLVKAFGSVIEVS
                     EDHLHQVTAITGSGPAFLYHVFEQYVKAGTKLGLEKEQVEESIRNLIIGTSKMIERSD
                     LSMAQLRKNITSKGGTTQAGLDTLSQYDLVSIFEDCLNAAVDRSIELSNIEDQ"
     gene            20540..21295
                     /locus_tag="NBH32_000849"
     CDS             20540..21295
                     /locus_tag="NBH32_000849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1514473.1"
                     /translation="MKGQHFIVTGGTSGLGLSIVRKLLENKVHVTLLVRDVDKATRIF
                     EQELGKTINVIPCDLNDLKSIQALQFEDNTSFDGFIYSAGLGYFKSISDHSFSEMIET
                     YQLNLISFNVLYTVLRPYLTSNAHIVGISSQAAFSTQANAAHYGASKAGFYALMNALR
                     LESPNIHIMTVNVGPIDTPFHQKADPSMKYAKKMGKIMLDANQLAEDIIYGIKTKQLE
                     INRPKWMHHALKMYQIAPRFFERCFPKLFKNKA"
     gene            21311..21559
                     /locus_tag="NBH32_000850"
     CDS             21311..21559
                     /locus_tag="NBH32_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514474.1"
                     /translation="MNCQSESVVRLCVRYAEQLSVFEEFTVLDILGDISVDQISDSTL
                     YYTCEKFKLLVLQGNVLGVQIITNNDESTCEVKYRKMF"
     gene            complement(21775..22683)
                     /locus_tag="NBH32_000851"
     CDS             complement(21775..22683)
                     /locus_tag="NBH32_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1514475.1"
                     /translation="MQKNILKSGISLSELGLGCMSLGTDLKKAEQIIDCAVENGITYF
                     DTADMYDKGINESVVGKALLKYQQRDDIVIGTKVGNRLTKDGSTTWDPSKSYIKEAVK
                     GSLKRLGIDHIDLYQLHGGTIDDPLDETISAFDELKQEGIIRAYGISSIRPNVIDYYL
                     KHSQIETIMSQFNLIDNRPESLLDAIHNNDVKVLARGPVSKGLLTSNSVNVLDNKFKD
                     GIFDYSHDELGETIASIKEIESNLSALTFSYLTSHDVLGSIIVGASSVDQLKENIENY
                     HTKVSLDQIKTARARVKDLEYTNHLV"
     gene            22765..23307
                     /locus_tag="NBH32_000852"
     CDS             22765..23307
                     /locus_tag="NBH32_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HCD1514476.1"
                     /translation="MDLNEKTIDRTVIYNGKIVDVEIHTVTLPNGETSTRELVYHNGA
                     VAVCAVTPKKEVVLVKQYRKPVEKPLLEIPAGKLEDDEDRVEAAKRELEEETGYIAKE
                     LTHVVDMYGSPGFCDEQLSIYFTDNLEEGTVHLDEDEFVEVIKVPIENVKSMLMNKEI
                     EDAKTIIALQHLLLNYNHSK"
     gene            23412..23861
                     /locus_tag="NBH32_000853"
     CDS             23412..23861
                     /locus_tag="NBH32_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1514477.1"
                     /translation="MEERLNRVKQQLQQSSYKLTPQREATVRVLIENEKDHLSAEDVY
                     LKVKDKAPEIGLATVYRTLELLAELKVVDKINFGDGVARFDLRKEGAKHFHHHLVCME
                     CGRVDEIDEDLLPEVENRVENEFNFKILDHRLTFHGVCETCQAKGKG"
     gene            23910..24797
                     /gene="xerD"
                     /locus_tag="NBH32_000854"
     CDS             23910..24797
                     /gene="xerD"
                     /locus_tag="NBH32_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCD1514478.1"
                     /translation="METIIEEYLRFIQIEKGLSSNTIGAYRRDLKKYQDYMTEHHISH
                     IDFIDRQLIQECLGHLIDQGQSAKSIARFISTIRSFHQFAIREKYAAKDPTVLLDSPK
                     YDKKLPDVLNVDEVLALLETPDLNKINGYRDRTMLELLYATGMRVSELIHLELENVNL
                     IMGFVRVFGKGDKERIVPLGDAVIEYLTTYIETIRPQLLKKTITEVLFLNMHGKPLSR
                     QAIWKMIKQNGVKANIKKTLTPHTLRHSFATHLLENGADLRAVQEMLGHSDISTTQLY
                     THVSKSQIRKMYNQFHPRA"
     gene            complement(24875..25381)
                     /locus_tag="NBH32_000855"
     CDS             complement(24875..25381)
                     /locus_tag="NBH32_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HCD1514479.1"
                     /translation="MQQALINFYYQFHTKQHYFLCHDILEDAWKAENNYSKQDAVVSL
                     ILFATACYHYRRNNLKGAYKSFNKSKEIIQNAKDRDALYLNLNDYQLLIEQQIAKLNA
                     AKPFSSVILPITPVFERIIKANYPDYDYNQETATDPFIVDHHMRRDRSEVIAAKEEAI
                     QLRKHRRN"
     gene            25473..26204
                     /locus_tag="NBH32_000856"
     CDS             25473..26204
                     /locus_tag="NBH32_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HCD1514480.1"
                     /translation="MYEVKLDAFNGPLDLLLHLIQKFEIDIYDIPMQALTEQYMQYVH
                     AMKQLEINIASEYLVLASELLMIKSKMLLPQSTSDMDVDDDPREDLVGRLIEYQNYKE
                     YTAILNDMKEERDFYFTKRPTDLSHLETDESWDPNHTIDLTELIVAYQRVKNRVELNT
                     PKSVEIRKETFTIQQATEQVTSRLKDKDHFNFFSLFTFSEPIEQVVTHFLAILEMSKA
                     GIINIEQQRNFEDINIIRGVNYHFG"
     gene            26197..26739
                     /gene="scpB"
                     /locus_tag="NBH32_000857"
     CDS             26197..26739
                     /gene="scpB"
                     /locus_tag="NBH32_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HCD1514481.1"
                     /translation="MDNHGILESLLFTAGDEGLDEKQLLEILDMSKDQLVELIENYSS
                     HGLMIQRFGTTYVLTTKKEAATYIEQLIEQKSQMKLSQAAMEVLSIIAYNQPLSRSDI
                     ELIRSINSDGAVKTLIAKGLVEAKVVNEQRSQQLITTDLFLNVFGISNIEDLPTTEED
                     DEEMDAFFSNLVNQKGENND"
     gene            26732..27469
                     /locus_tag="NBH32_000858"
     CDS             26732..27469
                     /locus_tag="NBH32_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1514482.1"
                     /translation="MTKELERLQKRIANSGYTSRRKAETLISEGKVKVNGTTVTELGT
                     KVKPSDTIEVEGIKIELEDKIYILFHKPTQVITSVSDDRGRTVVTDYFKDIEARIYPV
                     GRLDYDTSGLLLLTNDGEFTNLMTHPRYQIKKKYVAKLKGYLMREEVKALEKGIELED
                     GMTQPAEVKVKKQDKDKNTTLVEITITEGRNRQVRRMFEHFGHQVTKLSRIEYGPLNV
                     VGLNAGEGRVLTPHEVKVMRHLAEHGK"
     gene            27602..28327
                     /gene="srrA"
                     /locus_tag="NBH32_000859"
     CDS             27602..28327
                     /gene="srrA"
                     /locus_tag="NBH32_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator SrrA"
                     /protein_id="HCD1514483.1"
                     /translation="MSNEILIVDDEDRIRRLLKMYLERESFEIHEASNGQEAYELAME
                     NNYACILLDLMLPEMDGIQVATKLREHKQTPIIMLTAKGEETNRVEGFESGADDYIVK
                     PFSPREVVLRVKALLRRTQSTTVEQSEPHARDVIEFKHLEIDNDAHRVLADNQEVNLT
                     PKEYELLIYLAKTPNKVFDREQLLKEVWHYEFYGDLRTVDTHVKRLREKLNRVSSEAA
                     HMIQTVWGVGYKFEVKSNDEPAK"
     gene            28308..30059
                     /gene="srrB"
                     /locus_tag="NBH32_000860"
     CDS             28308..30059
                     /gene="srrB"
                     /locus_tag="NBH32_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SrrB"
                     /protein_id="HCD1514484.1"
                     /translation="MMSRLNSVVIKLWLTIILIVTTVLILLSIALITFMQYYFTQETE
                     NAIREDARRISSLVEQSHNKEEAIKYSQTLIENPGGLMIINNKHRQSTASLSNIKKQM
                     LNEVVNNDHFDDVFDKGKSVTRNVTIKEKGSSQTYILLGYPTKAQKNSHSKYSGVFIY
                     KDLKSIEDTNNAITIITIITAVIFLTITTVFAFFLSSRITKPLRRLRDQATRVSEGDY
                     SYKPSVTTKDEIGQLSQAFNQMSTEIEEHVDALSTSKSIRDSLINSMVEGVLGINESR
                     QIILSNKMANDIMDNIDEDAKAFLLRQIEDTFKSKQTEMRDLEMNARFFVVTTSYIDK
                     IEQGGKSGVVVTVRDMTNEHNLDQMKKDFIANVSHELRTPISLLQGYTESIVDGIVTE
                     PDEIKESLAIVLDESKRLNRLVNELLNVARMDAEGLSVNKEVQPIAALLDKMKIKYRQ
                     QADDLGLNMTFNYCKKRVWSYDMDRMDQVLTNLIDNASRYTKPGDEIAITCDENKSED
                     ILYIKDTGTGIAPEHLQQVFDRFYKVDAARTRGKQGTGLGLFICKMIIEEHGGSIDVK
                     SELGKGTTFIIKLPKPE"
BASE COUNT        10796 a         4284 c         5613 g         9533 t
ORIGIN      
        1 attttaagca attgttatgc gtaatatgaa aaatatttat aaatagataa ccaagataga
       61 taaaccatgt ttaccctttt tacgtaaagg tgtttagtag tcgtaaatca gtgcatgtgt
      121 taaaataaat gtataaatat tcgtaatcag aggtgtttac tgtgcccaaa aaatcggtta
      181 ggcatataaa aattagagaa attatttcaa atgaacagat agagacacaa gatgaattag
      241 ttaaacgatt aaacgattat gatttaaatg tcactcaagc aactgtttct cgtgatatta
      301 aagaactaca acttattaaa gtacctatac cttcaggtca atatgtttat agtttaccaa
      361 atgatagaaa attccatcct ttagaaaaat tgggacgtta tttaatggat tcctttgtta
      421 atatagatgg tactgataat ttacttgttc taaaaacatt acctggtaat gcacaatcta
      481 ttggagctat attagaccaa atcaattggg aagaagtact aggcacaatt tgtggtgatg
      541 atacttgttt aattatttgt cgaagcaaag aggcaagtga tgaaatcaag tcaagaattt
      601 tcaatttgtt ataaggatgc gatgagttta tgttacaaac cttatcaatc aagcaatttg
      661 ctattattga agagttagaa attcaatttt ctgatggttt aactgtttta agtggtgaaa
      721 cgggttcagg taagtcaatt attatagatg caattggaca attaattgga atgagagctt
      781 catctgattt tgtaagacat ggtgaaaaaa aggctgtcat cgaaggtata tttgatattg
      841 acgagagtaa agatgctata catatattga aaaatatgga tattgatgta gatgaagatt
      901 ttttattagt taaacgggaa atttttagct ctggtaaaag tctttgtaaa attaataatc
      961 aaacagttac tttacaagat ttacgaaaag ttatgcaaga attattagat attcatggcc
     1021 aacatgaaac gcagtctttg ttgaaacaaa aataccattt aacattattg gataattacg
     1081 cagaatcacg ttatcaagat ttgttggata aatatcacca aacttttcaa aattataaag
     1141 ccaaaaagca agagttagaa gatttagaat cagcagacca agcattgctg cagcgtttag
     1201 atttaatgaa atttcaatta gaagaattgt ctgaagcaca cctaaaagaa ggtgaaatag
     1261 agcaacttga aattgatatt aaacgcattc aaaactctga aaaattaagc ttagcactta
     1321 ataacgcaca tatgacatta acggatgaaa atgctatcac tgatcgtttg tatgaactaa
     1381 gcaaccattt gttaacaata aacgatatag ttccaaacaa atatgacaaa ttaaaagaag
     1441 atattgatca attttattac attttagaag atgcaaaaca tgagttatat gatgaaatgg
     1501 ctaatacaga attcgacgaa caagtgttaa acgagtatga atctcgtatg aatttgctga
     1561 ataatttaaa acgcaaatat ggaaaagata tttcagaatt aatctcatat caagaaaaac
     1621 ttaataatga aatcaataaa attgaaaact atgaacaaag tacatcgcag ttacgagaag
     1681 aaataaatgc attgtataat caagttatag aggttggaca agcgctatca aagcagcgtc
     1741 gtatagtcgc tagggagtta agagaccaca ttgtatcgga aattcaaaac ttacaaatga
     1801 aagatgcaaa tcttgagatt tcatttaaaa aattagaaga accgaatatt gatggaatcg
     1861 aatttgtaga atttttaatc agtcctaata aaggggaacc attaaaaagt ttaaataaaa
     1921 ttgcgtcagg tggagaactt tctagaatta tgttagcgtt aaaaagtatt tttgttaaat
     1981 cgagaggtca aactgcaatt ttattcgatg aggttgactc aggtgtatct ggacaagctg
     2041 cacaaaaaat ggcagagaaa atgcgagata tagcagaata tatacaagtt atttgtattt
     2101 ctcacttgcc acaagttgca tcgatgagcg accatcattt attaatttcg aaatcatcaa
     2161 aagatgatcg tacaacaaca caagttcaag agttaattgg cgatgataaa gtagatgaaa
     2221 ttgcacgaat gatttcaggt gcaagtgtta ctgatttaac tcgagaaaat gcaagagaaa
     2281 tgatacaaca caatcaaaga cgtagataat ttccaaagtg tgttgaatac tcatttgtgg
     2341 acttatttat gtgcattcaa actaaataga caatcatgta aaataggtaa ttaatggaca
     2401 tgaaaactat gtattaataa attttcaatg gatatatatc attagttagg agtaagaaca
     2461 tgtcagagaa acaatatgat ttagtcgttc tcggtggagg tactgcagga tatgttgctg
     2521 cgatacgtgc gtctcaatta ggtaagaaag tggcaatagt agaacgacaa ctattaggag
     2581 gtacttgtct ccataaaggt tgtattccta ctaaatcact gcttaagtca gcagaagtat
     2641 ttcaaacagt aaaacaggca gcaatgtttg gcgttgatgt caaagatgct aatgttaatt
     2701 ttgaaaatat gttggcacga aaagaagaca ttattaatca aatgtatcaa ggtgtaaagc
     2761 atttaatgca acacaatcac attgacattt ataatggcac gggacgtatt ctaggtacat
     2821 ctatattttc acctcaaagt ggcacaattt ctgtcgaata tgaagatggt gaatcagact
     2881 tattacccaa ccaatttgta ttaattgcaa ctggttcttc gcctgcagag ttgccatttt
     2941 tatcatttga tcatgataaa attttatcta gcgatgatat attatcatta aagacgttac
     3001 catcaagtat tggtattatt ggtggtggtg ttatcggaat ggaatttgca tcattgatga
     3061 tagatttagg tgttgacgta acagttatag aagcaggtga aagaatttta ccaactgaaa
     3121 gtaaacaagc ttcacaacta ttaaaaaaat cattatcagc acgtggtgtt aaattctatg
     3181 agggaataaa actttctgaa aatgatataa atgttaacga agatggtgtt acatttgaaa
     3241 tttcctcaga cataattaaa gtagataaag tgttgctatc gattggtaga aaacctaaca
     3301 catcagatat tggtttaaac aacactaaaa taaaactttc aatatcagga catattttaa
     3361 cgaacgaatt tcaacaaact gaagataaac atatttatgc agctggtgat tgtattggaa
     3421 aattacaatt ggcacatgtt ggatcaaaag aaggtgttgt ggccgtcgat catatgtttg
     3481 aagggaaccc aatcccagta aactataaca tgatgcctaa gtgtatttat tcacaacctg
     3541 aaattgcttc tatcggttta aacattgagc aagcaaaggc agagggaatg aaagttaaaa
     3601 gttttaaagt accatttaaa gcaattggta aagcagtgat tgatagccat gatgcaaacg
     3661 aagggtatag cgaaatggtg attgatcaat caactgaaga aattgtgggt attaatatga
     3721 ttggtccaca tgtaacagaa ttgattaatg aggcatcact gttacagttc atgaatggct
     3781 cggcattaga attaggacta acaacacacg cacatccttc catctctgaa gtgttgatgg
     3841 aattaggatt gaaagcagaa agtagagcta ttcacgtata aataggagga tttaacatga
     3901 ttgattataa atcattaggc cttagcgaag aagacctaaa agtaatatat aaatggatgg
     3961 atttaggtag aaaaatagat gaaagactgt ggttattgaa ccgtgcaggt aaaattccat
     4021 ttgttgtaag tggtcaagga caagaagcaa ctcagattgg gatggcatac gcattagaag
     4081 aaggtgatat tactgcgcct tattatagag atctagcttt cgttacttat atgggtatat
     4141 cagcgtatga tacgttttta tcagcgtttg gaaagaaaga cgatgttaac tctggtggaa
     4201 aacaaatgcc atcacacttt agtagtagag ctaagaatat tttgtcacaa agttcaccgg
     4261 tagcaactca aataccccat gctgttggtg ctgctttagc tataaaaatg gatggtaaga
     4321 agaaaattgc aacagcaact gttggtgaag gtagctcaaa ccaaggtgac ttccatgaag
     4381 gtttaaactt cgcgggtgta cacaaacttc cttttgtatg tgtcattata aataataaat
     4441 atgcaatttc agtacctgat tcattacaat acgcagctga aaaattatcg gatagagcac
     4501 ttggttacgg tatacatggt gaacaagtag atggtaatga tccattagca atgtataaag
     4561 cgatgaaaga agcaagagat cgagctattt ctggtcaagg ttcaactttg attgaagcag
     4621 taacaagtcg tatgacagct cattcatcag atgatgacga ccaatatcgt acgaaagaag
     4681 agcgtgaagc gcttaaaaaa gcagactgca atgaaaagtt caaaaaagag ttgctttcag
     4741 ctggcattat cgatgatgct tggttggcag aaatagaagc ggagcataaa gacatcatca
     4801 ataaagcgac aaaggctgct gaagacgcgc catatccaag tgtcgaagaa gcctatgcat
     4861 ttgtgtacga agaagggagt cttaactaat ggctaaatta tcttatttag aggcgatacg
     4921 ccaggcacaa gacttagcat tacaacaaaa taaagatgtg tttattcttg gtgaagatgt
     4981 tggtagaaaa ggtggcgtat ttggtactac tcaaggactg caacaaaaat atggtgaaga
     5041 cagagttatc gatacgccat tagctgagtc aaatattgtt ggaactgcca ttggtgcagc
     5101 tatggtaggt aaaagaccaa ttgcagaaat tcaatttgca gattttattt tacctgcgac
     5161 aaatcaaatt attagtgaag ctgcgaaaat gcgttatcgt tcaaataatg attggcaatg
     5221 cccattaacg attcgtgcac catttggtgg cggtgttcac ggaggactat atcattcaca
     5281 aagtattgaa agtatatttg cttcatcacc aggtttaaca attgttattc cttccacacc
     5341 gtatgatgct aaaggtctat tattatcttc aattgaatca aatgatcctg ttttatactt
     5401 tgagcataaa aaagcttatc gtttcttaaa ggaagaagtg ccagaagaat attacactgt
     5461 acctttagga aaagcagatg ttaaacgtga aggtgaagat ttaacagtat tttgttatgg
     5521 attaatggtc aattattgct tacaagccgc tgatatttta gctgcagacg gtatcaatgt
     5581 tgaagtcgta gatttacgaa ctgtttatcc attagataaa gaaacaatta ttgatcgtgc
     5641 taaacataca ggtaaagttt tacttgtaac tgaagataat ttagaaggta gcattatgtc
     5701 agaagtgtca gcgattattg cagagcattg cttgttcgat ttagatgcac caatcatgcg
     5761 tttagctgct ccagatgtac catctatgcc attttctcct gtattagaaa atgaaattat
     5821 gatgaatcca gaaaaaatct taaataaaat gcgtgaatta gcagaattct agggagggaa
     5881 agtcatggaa ataacaatgc ctaagttagg tgagagtgtt catgaaggca ccattgaaca
     5941 atggttagtt tctgttggtg atcatattga tgaatatgaa ccattatgtg aagttattac
     6001 agataaagtg acagctgaag tcccttccac gatatcagga acaattacag aaattttagt
     6061 tgaagcgggg cagacagtag ctattgatac aattatctgt aaaattgaaa ctgctgatga
     6121 aaagacaaat gaaacaactg aagagataca agcaaatgtg gatgagcata ctcagaaatc
     6181 tactaaaaaa gctagtgcaa cagtggaaca gacatctact gctaaacaaa atcaaccacg
     6241 taataatggt cgcttttcac ctgttgtatt taaactcgct tcagagcatg acattgattt
     6301 atcacaagtt gtaggtagtg gatttgaagg tcgtgtaact aagaaggata taatgtcagt
     6361 tattgaaaat ggtggtacca cagctcaatc tgacaaacaa gttcaaacac aatcaacatc
     6421 agtagataca tcaagtaacc aatcatctga agacaatagt gaaaacagca caataccagt
     6481 aaatggtgtg cgtaaagcaa ttgcgcaaaa tatggttaat agtgtaacag agattccaca
     6541 tgcatggatg atgattgaag tagatgctac aaatcttgtg aatacgagaa atcattataa
     6601 aaacagcttt aaaaataaag aaggatataa tctaacgttt tttgctttct ttgtaaaagc
     6661 tgtagcagat gctttaaaag catatccttt attaaatagt agctggcaag gaaatgaaat
     6721 tgtcttacat aaagacatta atatttcaat tgctgttgct gatgaaaata aattatacgt
     6781 acctgtgatt aagcatgcag acgaaaagtc aatcaaaggt atagctagag aaattaatac
     6841 tttagcaaca aaagcgcgta ataagcaatt gacagctgaa gatatgcagg gcggtacatt
     6901 tacggtaaat aatactggta cgtttggttc tgtatcatca atgggtatta taaatcaccc
     6961 tcaagcagcg attttacaag tagaatcaat cgttaaaaag ccagtagtaa ttaatgatat
     7021 gattgcaatt cgtaacatgg ttaatttatg tatttcaatt gatcatcgta ttttagatgg
     7081 tttacaaaca ggtaaattta tgaatcatat taaacagcgt atcgaacagt atactttaga
     7141 aaatacaaat atatattagt gataacatag atgcatctat cgacaacttg ttttatcttg
     7201 ttcttgtcga tggatgtatt tttttgggca ataaaatatg tgcaatatat tcaaaaaaag
     7261 ataaagaaca ataatcaaca tggttgaatg aatttttgca gtaaagtcaa aataagacat
     7321 catacttgaa acatattaat gaaaacaggt gaacaaatta gtttccatga ttttaagcac
     7381 aataatgttt ggtatattgt taaaattgtg tctaaatata ggtgtgattc agattagttt
     7441 attgaacaat atgttattaa ttagtagaat gaggatagtt taaatataaa gggataggtg
     7501 attgaactta tggacatgaa tttcgattta tacatgaacg gtgttgtaga acaagcaagg
     7561 aatgaaattg aatctgcggg atatgagcaa ttaactactg cagaagatgt tgacaaagtt
     7621 cttaaacaag atggtacaac actagttatg atcaattctg tatgtggatg tgcaggtggt
     7681 atcgcaagac cagcagcatc acatgcttta cattatgacg tattacctga tcgtctagtg
     7741 acagtatttg ctggacaaga taaagaagcg acacaaagag cgcgtgaata cttcgaaggt
     7801 tatgcgcctt caagtccgtc atttgcatta gtaaaagatg gaaagattac agaaatgatt
     7861 gaaagacatc aaatcgaagg tcatgatgtg atgaacgtaa ttaatcaatt acaaacatta
     7921 ttcaataaat attgtgaaga aagataagag gcgctaaccc atgttaaagt taaatcctta
     7981 caagattgga tttagaacaa taaaaacagc agtgggtatg actttaggtg taattattag
     8041 taagctgtta ggtttagata attatgcttc aagcgccata ttagtcgtat tatgtattaa
     8101 acatacaaaa gtacattcgc tacaagcgat tatttcaaga ttagtatcat gttttttagt
     8161 attgttttta ggttcagcaa tatttagttt attaggccag agtccaattg tactcggtat
     8221 tatcgtattg ttatttatac cattaactgt cgtattaaaa gtacaagaag gtgtcattac
     8281 gagttgcgtt atattacttc atgtttttaa tgcaaaatca attgatgtac atttaattgt
     8341 taatgaaaca ttattactgt taattggact aagcattgca tttacaatga atttaatgat
     8401 gccaagttta gacaaacaac tagacgaata caaatgtaaa attgagcaac aaattgctga
     8461 tatttttagt aaatatagtt atatttgtga aaaatatgaa gataccattg cgattgaatt
     8521 tgaagcgtta cttttaaata ttaaaaaggc gaagtctatc gcgttccgag atgttaaaaa
     8581 tcattttgtt agaaacgaaa attcatacta tcattatttt gatatgcgag aagagcaagt
     8641 ggaattgtta atgagaatga aaccgctcat cgaaagtatc tgtcataaag atccactttt
     8701 aaatgatgtt gcgtcactgc tgaaagaaat tggtctaaat gtgaatagta atgactatac
     8761 agcaatgcgt ttacacaatt tgtatgagac gcgtttgaaa ttggatgaac tgccattgcc
     8821 aacaacgcac aaagcgctta actcgagagc aagtattatt caaatattaa atgaattaga
     8881 agaatattta caaatcaaat cgcaatttgg ttcattgaaa atgcatagtg aagtagctta
     8941 aaaaataatc catccgtatg acccatgtat gaaagtaatc gttgcaatac gataacttat
     9001 taatacatgc gtttataacg gatggatttt taattgtatg attttgtata ggtagttgag
     9061 atatagttta taaaattgga atgatactcg ttaataacaa tgctaataca actgatacaa
     9121 gcataacgat aatgataatt cttaaaactt tattttgcat tttgtgtgcc tccaaatgaa
     9181 taacctttgt attaataatt acgacataag atataaaact tttaacttga gacgatataa
     9241 ctagcaatca tcataaagga atataataaa aaacatgagt aagtttgctt atgtcatttt
     9301 cctcattata cgatatcatt gaaaaatcaa tcaatatatt tttgacaaag cttagtttga
     9361 gttacaataa atatatatat taaattgaaa ataatatgac acaatttaca agcctgataa
     9421 atctgtgtaa cgtcaattac tgtaacattt tatttcatca atttttaccg tttggcgtac
     9481 ttgaaaattt tgataaaagg ggttccatta tgattaatga gcaaagatta cttaatacgt
     9541 ttttagaact tgtacaaatc gattctgaga cagggaatga atcaacaatc caacctattt
     9601 taaaagaaaa attcatagca ttagggttag atgttaaaga agatgaagcg gctaaacatc
     9661 ctaaattagg tgccaataat ctagtgtgta caatgaatag tactatcgaa gaaggcgaag
     9721 tacctaaatt atatttgacg agccatatgg atactgttgt acccgcaatt aatgtaaagg
     9781 caattgtaaa agaagacggc tacatatact ctgatggtac gactattcta ggtgcagatg
     9841 ataaagcagg attagcagcg atgcttgaag ttttgcaagt gattaaggaa caacaaatcc
     9901 cacatggaca aattcaattt gtgattactg ttggagaaga atcaggttta attggtgcta
     9961 aagaattaaa ttcagagttg ttggacgcgg actttggtta tgctattgat gcaagtgctg
    10021 atgtcggcac tacggttgta ggtgcaccga cgcaaatgtt aatttcagct aaaattattg
    10081 gcaaaacagc tcatgcaagt acgccaaaag agggtgttag tgctattaat attgcggcga
    10141 aagcaattag tcgaatgaaa ttaggacaag ttgatgaaat aactactgcc aatattggta
    10201 agtttcatgg cggttcagct acgaatattg ttgcagatga agtcatttta gaagcagagg
    10261 cacgttctca cgatccagaa agaataaaaa cacaagtcaa acatatgact gatgtgtttg
    10321 aaactactgc atcagaatta ggtgggaaag cagaagttac agtggagcaa agttatccag
    10381 gatttaaaat taatgataat gaagcggttg ttaaaattgc acaagaaagt gctcgtaatt
    10441 taggattatc tgcaaataca attatttctg gtggcggttc agacggcagt attattaata
    10501 catttggtat tccttcagtc attttaggag tgggttatga aaaaatccat acaacaaatg
    10561 agagaatgcc gattaaatca ttgaacttgc ttgcaagtca agtgttagaa ataatcaaaa
    10621 tagttgcgcg acaatcgaaa tgatttagtg tagtgtatgt ctattttgtg atacaatact
    10681 aatgaaaaat caacaagtga ggtaagtaat atgacacaac aaattggagt tattggttta
    10741 gccgttatgg gtaaaaacct agcttggaat attgaatcac gcggatatag tgtatctgta
    10801 tttaaccgct caagtgaaaa aactgattta atggttgaag aatcaaaagg gaaaaatatt
    10861 catccaacgt attcattgga agaatttgtt aattctttag aaaaaccacg taaaatttta
    10921 ttaatggttc aagcaggtaa agctacggat gcaacgattg atagtttgtt acctttatta
    10981 gatgatggag atattttaat tgatggtggt aatacaaact atcaagatac tatcagacgt
    11041 aataaagcat tagcacaaag tgcaatcaac tttattggta tgggcgtttc tggtggtgaa
    11101 attggagcgt taactggtcc gtcattaatg ccgggtggac aagaagaagc ttataacaaa
    11161 gttgcagata ttcttgatgc aattgctgct aaggcaaaag acggtgcttc atgtgttact
    11221 tatattggtc caaatggtgc tggacactat gtgaaaatgg tacacaatgg tatcgaatat
    11281 gctgacatgc aattaattgc agaaagttat gctatgatga aagaattatt aggcatgtct
    11341 catgaagaca ttgctcaaac gttcaaagac tggaatgctg gtgaattaga aagttattta
    11401 attgaaatca ctggtgatat tttcatgaaa ttagatgaaa ataaagaagc attagttgaa
    11461 aaaattcttg atactgcagg tcaaaaaggt acaggaaaat ggacttcaat taacgcatta
    11521 gaattaggta ttccattaac aatcattact gaatctgtat ttgcacgttt tatctcttca
    11581 atcaaagaag agcgcgtaaa tgcttctaaa gaattaaatg gtccaaaagc atcatttgat
    11641 ggtgacaaga aagacttttt agaaaaaatt cgtaaagcat tatatatgag taaaatttgt
    11701 tcttatgcac aaggtttcgc gcaaatgcgt aaagcaagtg aagataatga atggaattta
    11761 aaacttggtg atttagctat gatttggaga gaaggttgca ttatccgtgc acaattctta
    11821 caaaagatca aagatgctta cgataataat ccaggattac aaaacttatt gctagatcct
    11881 tactttaaaa acatcgttac tgaatatcaa gatgcgctac gtgatgttgt cgcaactggt
    11941 gttcaaaatg gtgtaccaac acctggtttc tcttcaagta ttaattacta tgatagttac
    12001 cgtgcagctg atttacctgc aaacttaatt caagcacaac gtgactactt cggtgctcac
    12061 acgtacgaaa gaaaagataa agaaggcgta ttccatacac aatggattga agaataatat
    12121 tgattaaagg atagttgaaa gatactatcc ttttttatat cttttgataa atataaaaca
    12181 gagtgtgata gagtatctac aacctataaa aaattacaca agtattatcc ttgaccttaa
    12241 aagcatcccg acatattttt aagcattttg tgaagtaata aaatatttac tgtgttatga
    12301 tagcatgtac tatagagaaa tagaggtatt aacttatgga aaagttaaat caagttatgt
    12361 tatatgttga tgatgttgac aaagcgaaag cattttggac agaaacttta gaatttgttg
    12421 ttgtaagtga aacaccgtta gctgaggact atgttgcagt tgaagtatca cctacgaaag
    12481 atgctgaaac gtcgttgacg attatggcaa aagaatttat tgaaaagtat agtccagagg
    12541 tgaacctagg aacaccatct ttaatgttta aagagaaaaa ctttgatgca ttatactcaa
    12601 aattaaatga tttaggttta actgggcatg atatagtaga aatgaatggt caacgtgcat
    12661 ttaatttcca agatggccaa ggtaactatt ttgcagttag tgactaaaca atcgatttta
    12721 atatttatac aaacctttat attgattttc ggattgaatt tcagtatgaa ggttttattt
    12781 ttatcgtttt atgcaatcgt ttgcacaaat cttttgactt ttttagaaag tgtgactaaa
    12841 ctggtattat acaatggttg gaagcgtttt ccagcgcagt gattaacagg aggtgattaa
    12901 atggttacga ttaaagatgt tgcactaaaa gccggtgttt ctccttcgac agtttcaagg
    12961 gttataaaag gaaatcaacg tattagcgaa gcgacaattt caaaagtgaa gaaagttatg
    13021 gaagaattga attattttcc taatactgct gctagaactt taattactaa ccaaacatat
    13081 aaaattggtt tagtgttaaa agggtctgag gagcctattc gactgaatcc attctacatc
    13141 aatgtattgc taggaatttc tgaaacgtgt aaccagcatg gctatggtac acaaacgaca
    13201 gtctcaaata atttgaatga tttaatggat gaagtttata aaatgattaa acaacgaatg
    13261 gttgatgcgt ttatactgct ctattcaaaa gaaaatgatc cgattaaaca aatgttaatt
    13321 gatgaaagca tgccatttat tgtgattggt aagcctacat cggatataga tcatcaattt
    13381 acacacatag ataatgataa tatattagct tctgaaaatt tgacacgaca tgttattgaa
    13441 caaggtgtag atgaattaat atttattaca gaaaaaggaa attttgaagt ttcaaaagat
    13501 agaataaaag gatttgaaac gatagcttct caaaataaaa ttaattatca aattattgag
    13561 actagtaatg aaagagaagt tatttttaat tacatgcaaa atctacatac gcgtttgaag
    13621 gacccaaata ttaaacaggc aatcatttcg ttagatgctg tgttacattt agcgatttta
    13681 agtgtcctat atgaacttaa tattgaaatt ccgaaagatg taatgacagc aacgttcaat
    13741 gattcttatt taaccgaaat tgcgtcacca cctcaaacca gtattgacat cgagcctcga
    13801 atgttaggtc aacaggctgg tgcagcaatt ttaaatatat taaaaaataa agcacaggat
    13861 gttattgaac tcgtcattat agatacagaa ttaaaaataa gaaaatcaac acagcgatag
    13921 caaaggagtt ttaaaatgaa taagcaatgg tggaaagaag cagtagcata tcaagtatat
    13981 ccaagaagtt ttaatgatag taatcacgat ggtattgggg atttacctgg aatgattgat
    14041 aaattggact acttaaaaga tttaggtatc gatgtcattt ggctcagtcc aatgtttaaa
    14101 tcacctaatg atgacaatgg ttatgatatt agtgactatc aagaaattat ggatgaattt
    14161 ggaacgatgg aagactttga tcgtttatta aaaggtgttc atgatagagg catgaagctt
    14221 attttagatt tagttgtaaa tcatacatct gatgaacatc cttggtttat agaatctaaa
    14281 tctagtaaag acaatcccaa acgtgattgg tatatttggc aagatccaaa gccagatggc
    14341 tctgaaccta acaactggga aagtatcttt aatggatcta catgggaata tgatgctaat
    14401 actgagcaat attatttcca tttattcagt aaaaaacaac ctgatttgaa ttgggctaat
    14461 ccagaagtta gagatgctgt atttgaaatg atgaactggt ggtttgataa aggcattgat
    14521 ggatttagag tagatgcaat tacgcatatt aaaaagacgt ttgaagcggg tgacttacct
    14581 gtacctgagg ataaaacata tgccccagca tttgatgtag atatgaatca gccaggtata
    14641 caaacttggt tacaagagat gaaagatcgc tcattaagta agtatgacat tatgactgtt
    14701 ggtgaagcga atggtgtaag ccctgatgat gctgatgact gggtcgggga agaaaatggt
    14761 aaatttaata tgatattcca atttgaacat ttgggactgt ggaatagtgg tgattctcac
    14821 tttgatgtaa attcgtataa atctgtatta aatagatggc aaaaacaact tgaaaataaa
    14881 ggttggaatg cgttgtttat tgaaaatcac gaccaaccac gacgtgtatc gacgtggggt
    14941 gacgatgaca agtattggta tgaatcagca acaagtcatg cagctgttta tttcttgcaa
    15001 caaggtacgc cattcattta tcaaggtcaa gaaattggta tgacgaatta tccatttgaa
    15061 agtattgaaa cgtttaacga tgttgctgtt aaaaatgact atcaaattgt gaaagctcaa
    15121 ggtggagatg tagacgcttt acttgcgaaa tataaagatg agaaccgaga taattctcgc
    15181 acaccaatgc aatgggatga tacgttaaat ggaggattta caaatggtga accgtggttc
    15241 ccagtgaatc cgaattataa aactatcaat gttgcacaac aattagaaga tgagcattca
    15301 gtattacaat tttataaaga tttaattcaa ttaagaaagt ctaatgatgt atacgtatat
    15361 ggtcaatttg atttagtaga tgctgaaaat tcacaagttt ttgcgtacac gagaacatta
    15421 aatgaaaagc aagttcttat tgtaggtaat cttactaacc acgaagcaga attaactgta
    15481 ccatttgatt taagccatgg agaagtaaag ctatttaatt atgatgccaa agttaattta
    15541 aaacagttac gtccatatga agcatgtgtt atcgaactaa attaacatat taatatgccc
    15601 cctaatagaa tctaaggcca tttgtagacc tatagatata ttagggggct tctttgtttt
    15661 taatttttaa tcttcgtcat aaggactctg tttaacagga atccaaagct gaattttagt
    15721 gaatgggtca ttaaatgaaa tatcgagagg gtacacttca acatataaat catttcgttc
    15781 atatggcaat gttaactgca aacttgattc aatatagtac caagcttcat tagttgcata
    15841 atcaatttca ccttgtaaat tgaatttcgc acaatgtttg ccaggtaaaa atcggctttc
    15901 taagtgtgca ggataacgtt cacttggtac acctacaaat atttctaaac cattttcaag
    15961 aggacaacta ataacaaata gttcaaatgg actaacgtca ttatatcgtc gtaactcttt
    16021 aattttacca tcaatgagca agtcttctaa aaaatccgga acattaaaag gatgtgacaa
    16081 atacttagtg tctataaatc gtgcatatcc aaccaatgaa atatcatctg tctcttctaa
    16141 tctgtatgga tagggtgctc tctcagtagt tgataatttg atatataatc gctcttgaat
    16201 ttgtaattca tcttttttag tagaagcttg aataggtgat acgccgtgaa aatcactaaa
    16261 atcatttgca aaatcatttg aatttgcata gtgatatttt ttagcgatat ctacaagtcg
    16321 tgtagcacca ttaatcacat catttgcagc gagtgtcatt ttacgcgcgc gtgcataagc
    16381 ttctggagat aagccgacaa tcattttaaa tgattgatca agatggtatg gcgaaagacc
    16441 aacgtaatca cttaattctt gcaaattgaa aggctctaat aaacgatctt caatataaac
    16501 aattgcctgt tgtatttgct tgataacgtc caaaactttc actccaatta attcaatcat
    16561 ttatttttat tctacattat ttctataaat tatacaccca tttgttcaat gattattaaa
    16621 atagttttgg gcattgtaaa atataatttc ataatatagt ctagaaaaaa agcgaatgat
    16681 agaacaattg atttacttga ttcgtaatca atccttgtca ttcgctcatt tatttttgtt
    16741 taacatgtgc gttttaattc aattattgaa tatcgtccca ccaatggtta ccatcacgag
    16801 caagtagtaa atcactttct aatggaccat tagtacctga ttcatagtta gggaattctg
    16861 gatcaaccat attccattca tcttgaattg catcaacaaa tttccatgtt gattttaatt
    16921 cttcccagtg cgtgaagtta gtggcatcac ctttaagaca atcgaataat agattttcat
    16981 atgcatctac agtattcatt ttatcttgag cgctcattga ataagacaat tgaacaggtt
    17041 cagtttcgat accttgtgta tttttcttag catttagatg taaagataca ccttcattag
    17101 gttggatatt gattactaat aggtttgaat ctaatagttt atcagtttca tagtataagt
    17161 tcattggtac ttctttaaat tcaacgacaa cttgaattgt tttagatttc atacgtttac
    17221 cagtacgaat atagaatggt acaccagccc atctaaagtt atcaattgtt aatttacctg
    17281 aaacaaaggt aggtgtgtta gagtcatctg caacgcgatc ttcatcacgg tatgctttaa
    17341 cttgtttacc atcgatatag ccttcgccat attgaccacg aacaaagttc tttttaacat
    17401 cttcagattg gaaatgacgc agtgatttaa gtacttttac tttctcagca cggatatctt
    17461 cactatttaa actaataggt gcttccatag ctaataatgc aaccatttgt aacatgtggt
    17521 tttgcaccat atcttttagc gcgccacttg attcataata accaccacga tcttcaacac
    17581 ctagtatttc agaagatgta acttggatgt ttgaaatata tttgttattc cataatggtt
    17641 caaacatcgc attcgcaaaa cgtaatacct cgatattttg aaccatgtct tttcctaaat
    17701 agtggtcaat acgataaatt tcttcttctt taaatgattt acgaatttga ttgtttaatg
    17761 cttcggctga ttttaaatca ctaccgaatg gtttttcgat aacaaggcgt ttaaatcctt
    17821 ttgtatcagt aagaccagaa gattttagat aatcagaaat aacgccaaag aattgtggtg
    17881 ccattgctaa atagaatagt cgattacctt ttaattcaaa ttggctgtct aattcattac
    17941 taaaatctaa taattcttga tagctttctt cattactaac atcatgtcta tgatagaaga
    18001 catgttccat aaacgcgtca attttgtttg tatcttttac gtgcttttga attgatgatt
    18061 ttacttgatt acggaaatca tcattagtaa tgtcacgacg tccaataccg atgatggcaa
    18121 tatgttcatc taaattgtct tgttggtaga gatggaatat tgatggaaac aacttacgat
    18181 ggcttaagtc accagttgca ccaaagattg tgattaaaca agggatgtgt ttgtttttag
    18241 tactcaagat taaaacctca attcttaatt agatatatga tttattatta taagataatc
    18301 cattcagcag gtcattcaat atgctcgaca gtatgcagtg tcttaaatga aatatcgatt
    18361 catgtatcat gtttaatgtg atacttatta atgataagta taacgtaatt atcaaaattt
    18421 atatagtttt gtctacgtta aagttagaaa aattaactag caaagacgaa tttttaacag
    18481 attttgattc aagtataaat taaaactaaa ttgatacaaa ttttatgata aaatgaattg
    18541 aagaaaagga ggggcatata tggaagttac attttttgga acgagtgcag gtttgcctac
    18601 aaaagagaga aatacacaag caatcgcctt aaatttagaa ccatattcca attccatatg
    18661 gcttttcgac gttggtgaag gtacacagca ccaaatttta catcatgcaa ttaaattagg
    18721 aaaagtgaca catatattta ttactcatat gcatggcgat catatttttg gtttgccagg
    18781 attactttct agtcgttctt ttcagggcgg tgaacagaag ccgcttacat tggttggacc
    18841 aaaaggaatt aaagcatatg tggaaatgtc tatgaattta tcagagtcac atttaaacta
    18901 tccaattaca tatattgaaa tcgatgatca tctgacatat catcatgatg gttttactgt
    18961 agaggcgcat ttacttaatc atggtattcc atcatatgga tatagagtaa tggcacctga
    19021 aactacaggt acaatcaatg ttgaagcgtt aaaaaacatt ggtcttgaac caggtcctaa
    19081 atatcaagaa gtaaaatctc atgatacatt tgaacataat ggtcaagttt atcaatcaaa
    19141 agattttaga ggtgagtcca aacagggtcc agttgtagca atttttggtg atacaaaacc
    19201 atgttctaat gaacgtgtga tttctagaga tgctgatgtg atggtacatg aagcgaccta
    19261 tatagatggt gaaaagcatt tagccaacaa ttatcatcat agccatattg aagacgtatt
    19321 tgcattgata aaagaagcca atgttaaacg tacgttgatt acacatttga gcaatcgcta
    19381 taatacagaa gatataaatg aaatttatca aacattgata caaaatgaag atacacctaa
    19441 ttttaatttt gtaaaagatt ttgatagttt taaagtctaa tgacaaagta ataaacaatg
    19501 cttgccaaat agtaataatt tataaaaata acccgtccaa ctatgtatgc gatgatgcat
    19561 acatgtgttg gcgggtttgt ttttattggt cttctatatt agaaagttca atactacggt
    19621 cgacggcagc gtttagacaa tcttcgaaaa tagatactaa atcatattgt gacaatgtat
    19681 caaggccagc ttgtgtcgta ccacctttag aggtaatatt ttttcttaat tgagccatgc
    19741 tcaaatctga acgttcaatc atcttacttg tacctataat aaggttgcgt atagattctt
    19801 caacttgttc tttttctaga ccaagtttcg taccagcttt aacgtattgc tcgaatacat
    19861 gatataaaaa tgctgggccg cttccggtga tagctgttac ttgatgtaaa tgatcttctg
    19921 atacttcaat tacagaacca aatgctttaa ctaaatcgtt aatttcatct ttagatttag
    19981 ggtcaaagtt gtttgaaaaa ctaatgccag taacagagtg tccgacttgc gcatttgtgt
    20041 ttggcataat tctagcaact ggattttggc attctaattg ttgtttaata taatcaatcg
    20101 gaatacctgc cataattgaa atgaagcaat tgtcttttgt gatatgtggt ttgatgcgtg
    20161 ttgctagagc atcaaagtca tgcggtttgg tacctaaaaa aacataatct gcatctttta
    20221 ataatgtcgc atcatcataa ctatagttaa cacctagttt ttcagcgaat gcttttaaag
    20281 cttgttcatt agatttattt gttaaatata tatcattggc atctaagttg cttgagttaa
    20341 taattcctgt aaatatagct tgtgccatat taccagctcc ataaaatacg agtttcatta
    20401 acgtcacatc cctttagttt ttgatttgta tgttatattt tcttattatt gtatatatat
    20461 taggaaagat aatcaacttg tctcaattat gtaaagttga taaattgcat tctatataat
    20521 tgaaaaggga ggttaattga tgaaaggaca acattttata gtgactggtg gtacgagtgg
    20581 tttaggttta tcgattgtcc gaaagttact tgaaaataaa gtgcatgtaa cgttgttggt
    20641 gcgtgatgtt gataaagcaa ctagaatctt tgaacaagaa ttggggaaaa ctataaatgt
    20701 tattccttgt gacttaaatg atttgaagtc gatacaggca ctgcaatttg aagacaatac
    20761 atcattcgat ggattcattt atagtgcagg tttaggttat ttcaaatcta tttcagacca
    20821 tagtttttca gaaatgattg aaacgtatca gcttaattta attagtttta atgttttata
    20881 tactgtgttg agaccatatt taacatctaa cgcacatatt gtaggtattt caagtcaagc
    20941 tgcattttcc actcaagcaa atgctgcaca ttatggggca tcgaaagcag ggttttatgc
    21001 attaatgaat gctttaagac tagagtcacc aaacatacat atcatgacag ttaatgttgg
    21061 cccgattgat acaccttttc atcaaaaagc cgatccatca atgaagtatg ctaaaaagat
    21121 ggggaaaatt atgcttgatg ctaatcagct tgcagaagat atcatttatg gcattaaaac
    21181 aaaacaattg gaaattaatc gccctaaatg gatgcaccat gcgttgaaaa tgtatcaaat
    21241 tgcgcctaga ttttttgaac gatgttttcc taaattattt aaaaataaag cataaatagg
    21301 tgggatggca atgaactgtc aatcggagag tgtggtaaga ctttgtgtac gatatgcgga
    21361 acaactatca gtgtttgaag aatttacagt gctggatatt ttaggcgata tttctgtaga
    21421 ccaaatttca gacagtacat tatattatac ttgtgaaaaa ttcaaactac ttgtgttgca
    21481 aggaaatgtg ctgggtgtac aaataataac taataatgat gaatcaacat gcgaagtgaa
    21541 atatagaaag atgttttaat aaaattatta aaagatgaag tgggagaagt ttgcttaagg
    21601 cactgccgca gggcgaaatt actaaatact gatgcgtgag gacaacgctt taatttctat
    21661 cataagtgag taatcttaat cataaattat agcgaattgt acataaacaa cagcagtaag
    21721 gcatttttat aatgaaacgc ctcatgctgc tgtttttatt actgaaaatg acttctacac
    21781 taaatgattg gtatattcca aatcctttac acgagctctt gctgttttaa tctgatctaa
    21841 actaacttta gtatgatagt tttcaatatt ttcttttaat tggtcgacgc tacttgcacc
    21901 tacaatgatg gaaccaagca cgtcatgtga tgttaaataa ctaaatgtca atgcagataa
    21961 attactttca atttctttaa tagaggctat tgtttcaccc aattcatcat gagaataatc
    22021 aaaaatacca tctttaaatt tattgtctag cacattaaca ctgtttgaag ttaataatcc
    22081 tttagacaca ggtcctcttg ccaatacttt aacatcattg ttgtgaattg catctaataa
    22141 tgattctgga cgattatcaa tcaaattgaa ttgagacatt atcgtttcga tttgactatg
    22201 ttttaaataa taatcaatta catttgggcg aatagaagaa ataccgtaag cacgtataat
    22261 tccttcttgt ttcaattcat caaatgcgct tattgtttcg tctaatgggt catcaatggt
    22321 tccgccatga agttgatata aatcgatatg atcgatacct aaacgcttta gtgaaccttt
    22381 aactgcctct ttaatatagg atttactcgg atcccatgtt gtactgccat cttttgttaa
    22441 acgattgcct acttttgtac caatgacaat atcatcgcgt tgttgatatt taagaagtgc
    22501 cttaccaaca actgattcat ttataccttt atcgtacata tctgctgtat caaaataagt
    22561 gataccattt tcaacagcac aatctataat ttgttcggct ttttttaaat ctgtacctaa
    22621 actcatgcaa cctagaccta attcagataa tgaaatacca ctttttaata tatttttttg
    22681 catgtctgtt ctcctttcga tacactttag ctaacaagca tagtgtatca atatataata
    22741 tgaatttaaa ggaacgtgat tataatggat ttaaatgaaa aaacaattga tcgaacagtt
    22801 atttataatg gcaaaattgt agatgtagaa attcatacag tgacattacc aaacggtgaa
    22861 acgtcaacaa gagaattagt ttatcataat ggtgcagttg ctgtttgtgc agtgacacct
    22921 aaaaaagaag tcgtattagt gaaacagtat cgtaaaccag tagaaaaacc attgctagaa
    22981 attccagctg gtaaattaga agatgatgaa gatagagtcg aagcggcaaa acgtgaatta
    23041 gaagaagaaa caggatatat tgccaaagag ttaacacatg ttgtagatat gtatggttca
    23101 ccaggttttt gcgatgaaca attatcaata tattttacgg ataatttaga agaaggtaca
    23161 gttcacttag atgaagatga atttgtcgaa gtcattaaag ttccgattga gaatgttaaa
    23221 tcgatgctaa tgaataaaga aatagaagat gcgaagacta tcattgcatt gcaacaccta
    23281 ttattaaatt ataatcattc taaataacaa atgagaaaag cttgcatttt attgagaaaa
    23341 ctgttagttt taattgtgaa gtttgaaata atttgtaatg attttaatta ttagtagggg
    23401 agtggacatc gttggaagaa cgattaaatc gcgttaagca acaattacaa caatcatcat
    23461 ataagctaac gccacaacgc gaagctactg ttagagttct aattgaaaat gaaaaagatc
    23521 atctaagtgc tgaagacgta tatctgaaag taaaagataa agcgcctgaa attggcttgg
    23581 cgacagtata cagaacgtta gagttgttag ctgaactaaa agttgtcgac aaaattaact
    23641 ttggtgatgg cgtcgctcgt tttgatttaa gaaaagaagg cgcaaaacat ttccaccatc
    23701 atttagtatg tatggaatgt ggtcgtgtag atgaaatcga tgaagatttg ttaccagaag
    23761 ttgaaaatcg agttgaaaat gagttcaatt ttaaaatttt agatcatcgt ttaactttcc
    23821 atggtgtgtg tgaaacgtgc caagctaaag gtaaaggata gtaaattgcg taggttaaat
    23881 taaccttcgc tttttttaga ggtgtggtta tggaaacaat tattgaagaa tatttgcgtt
    23941 ttatacaaat tgaaaaagga ctaagttcta acacaattgg tgcttataga cgtgatttga
    24001 aaaagtatca agattatatg actgaacatc atatctcgca tattgatttt atagatcgac
    24061 aattaattca agagtgtttg gggcatttaa tagaccaagg gcaatctgct aaatctattg
    24121 cgcgatttat ttcaacaatc cgtagttttc atcaatttgc tataagagaa aaatatgcgg
    24181 cgaaagatcc aacggtatta ttagattcac caaaatatga caaaaaattg cctgacgttt
    24241 taaatgttga cgaagtgttg gctttattag aaacgccgga tctaaataaa attaatggtt
    24301 atcgtgatcg tacgatgtta gaacttctgt acgcaacggg aatgcgtgta tctgaattga
    24361 tacatttaga gttagagaac gtgaacttaa taatgggatt tgtacgcgta tttggtaaag
    24421 gcgataaaga aagaattgta ccattaggcg acgcagtcat tgagtactta actacttata
    24481 ttgaaacgat tagaccgcaa cttttaaaaa agactattac cgaagtctta tttttaaata
    24541 tgcatggtaa acctttatcg cgacaagcaa tatggaaaat gattaaacaa aatggtgtaa
    24601 aggcaaacat taaaaagacg ttaacgccac atacgttacg ccactctttt gcgacacatt
    24661 tattggaaaa tggcgcagat ttaagagcag tgcaagagat gttaggtcac tctgacatat
    24721 ctactaccca actctataca catgtttcga aatctcaaat tagaaaaatg tataaccaat
    24781 ttcatcctag agcataaagt gaacaataac tcaaaagtca caatacatat gactaaaaat
    24841 gtctgtgcta ttgtggcttt tttaaattgg ttgattaatt acgtctatgt tttcttaatt
    24901 gaatcgcttc ttcttttgct gcaatcactt ctgaacgatc acggcgcatg tgatggtcta
    24961 caataaaagg atctgttgct gtttcctgat tataatcata gtctggatag ttggccttga
    25021 tgatgcgttc aaagactgga gttattggta atataacaga tgaaaaaggc tttgctgcat
    25081 tcaattttgc aatctgttgc tcaattaaca actgataatc atttaaatta aggtataacg
    25141 catctctgtc tttagcattt tgtattattt ctttagattt attaaaagac ttataggcgc
    25201 cttttaaatt attgcggcga taatggtaac aagcagttgc aaacaagatt aaactaacaa
    25261 ctgcatcttg cttactgtag ttattttcag ctttccatgc atcttctaaa atgtcatgac
    25321 ataggaaata atgttgctta gtatgaaatt gataatagaa atttatcagt gcctgttgca
    25381 ttttgttatc accccaattt aaaagtaagt tattttcatg ctataatatt ttagagaatt
    25441 atgcacatat gacgcaatac gaggtagata ttatgtatga agttaaatta gatgctttca
    25501 atggaccatt agatttattg ctgcatctta tccaaaaatt tgaaatagat atttatgata
    25561 ttcctatgca agcattaaca gagcagtata tgcagtacgt tcatgcaatg aaacagcttg
    25621 aaattaatat tgcaagtgaa tacctagtat tagcgtcaga actcttaatg attaaaagta
    25681 agatgctatt accacaatca acatcagata tggatgttga tgatgaccca cgggaagatt
    25741 tagttgggcg tttaatagaa tatcaaaatt ataaagaata tactgctatt ttaaatgaca
    25801 tgaaagaaga aagagatttt tattttacaa aaagaccgac agatttatct catttggaaa
    25861 cagatgaatc ttgggatcca aatcatacga ttgatttaac tgaattaatt gtagcttatc
    25921 aaagagttaa aaatagagtt gagttaaata cacctaaatc tgttgaaatc cgaaaagaga
    25981 catttaccat tcaacaagct acagaacaag tgacatcgag attgaaagat aaagatcatt
    26041 ttaacttctt tagtctgttt acgttttctg agccaattga acaagtagtc actcactttt
    26101 tagctatttt agagatgtca aaagcaggaa taattaatat tgagcaacaa cgtaattttg
    26161 aagatattaa cattattaga ggagtgaact accattttgg ataatcatgg tatattagag
    26221 tcgcttttat ttacagctgg cgatgaaggt ttagatgaaa aacaactatt agaaatatta
    26281 gatatgtcga aagaccaact cgttgaatta attgaaaatt attcatcaca tggattaatg
    26341 atacaacgat ttggaacgac gtatgtttta acgactaaaa aagaagcggc aacgtatatt
    26401 gaacaattaa ttgaacaaaa gtcacaaatg aaattatcac aagcagcaat ggaagtacta
    26461 tcaattattg cttataacca gccattatca agaagtgata ttgaattaat tcgtagtatc
    26521 aattcagatg gtgcggtcaa gactttgatt gccaaaggac tagttgaggc taaagtggtt
    26581 aatgaacagc gtagccaaca gttaattact actgatttat ttttaaatgt atttggtatt
    26641 tccaatatag aagatttgcc gacaactgaa gaagacgatg aagaaatgga tgctttcttc
    26701 agtaatctag tcaatcaaaa aggagaaaat aatgactaaa gaattagaac gattacaaaa
    26761 acgtatagct aatagcggtt atacttcaag acgtaaggca gaaactttaa tttctgaagg
    26821 aaaagtgaaa gtgaatggta cgactgtcac tgaattagga acaaaagtaa agccatcaga
    26881 tacgattgaa gttgaaggta ttaaaataga actagaagac aaaatttaca ttttattcca
    26941 taaaccaact caagtgataa caagtgtatc agatgataga ggacgtacag tggttacgga
    27001 ttactttaaa gatatagaag cacgtattta tcctgttggt cgtttagact atgatacttc
    27061 tggattgtta ttacttacaa atgatggtga atttactaat ttaatgacac atccaagata
    27121 tcaaattaag aagaaatatg tagcaaaatt aaaaggttac ttaatgagag aagaagtgaa
    27181 agccctagaa aaaggtattg aattagaaga tggtatgacg caaccggctg aagtgaaagt
    27241 taagaaacaa gataaagata aaaacacaac attggttgaa attacaatta cagaaggtcg
    27301 taatcgccaa gtcagaagaa tgttcgaaca ttttggtcat caagtgacga aactgtctcg
    27361 tattgaatat gggcctttaa atgttgtcgg tttgaatgct ggtgaaggac gtgtattgac
    27421 gcctcatgaa gtaaaagtga tgcgtcattt agcagaacat gggaaataat taaataaaat
    27481 atgtatttat cacaaagttt gagaattttt tcacaaaatt ttaggttttt aataactgta
    27541 tgcgctttcc tgtgatataa tacttttgta aagaaaagca tgtgtgggag gtatgacctg
    27601 tatgtcgaac gaaatactta tcgtagatga tgaggataga atcagaagat tacttaaaat
    27661 gtatttagaa agagaatctt ttgaaatcca tgaagcaagt aatggccaag aggcttatga
    27721 acttgcaatg gagaataatt atgcttgcat actactagat ttaatgttgc ctgaaatgga
    27781 tggtatccag gtggcaacta aattgcgtga acataaacaa acaccgatta ttatgttgac
    27841 tgctaaaggt gaagaaacaa accgtgttga aggttttgaa tctggtgcag atgattatat
    27901 cgtcaaacca ttttcaccaa gagaagtagt cttaagagtt aaagcacttc taagaagaac
    27961 gcaatctaca actgtagaac aaagcgaacc tcacgcacgt gatgtgattg aatttaaaca
    28021 tttagaaata gataatgatg cacatcgcgt acttgctgat aatcaagaag ttaatttgac
    28081 tcctaaagag tacgaattat taatatattt agctaaaaca ccaaataaag tatttgaccg
    28141 tgaacaatta ttaaaagaag tttggcatta tgaattctat ggtgatttaa gaacagttga
    28201 tactcatgtt aaacgactta gagaaaagtt aaatcgtgtg tctagcgaag ctgcgcatat
    28261 gattcaaaca gtctggggcg ttgggtataa atttgaggtt aaatctaatg atgagccggc
    28321 taaatagtgt cgtaattaaa ctgtggttaa ctattatttt aatagtgacg acagttttaa
    28381 ttttattaag tattgcttta attaccttta tgcaatacta tttcacacaa gaaactgaaa
    28441 atgccataag agaagatgct agacgtataa gttcactggt cgaacaatca cataataaag
    28501 aagaagcaat aaaatatagt caaacattaa ttgaaaatcc tggtgggttg atgattataa
    28561 acaataaaca tcgtcaatca acggcttcac tttctaatat taaaaagcaa atgttgaatg
    28621 aagtagtcaa caacgaccat tttgacgatg tgtttgataa aggtaaatct gttactcgaa
    28681 atgtaacgat taaagaaaag ggttcatctc aaacttatat tttgttaggc tatccaacaa
    28741 aagcacagaa gaatagtcat agcaaatata gtggagtatt tatatataaa gacttgaaat
    28801 caatcgaaga tacaaataat gctattacga ttatcactat aattacggct gttattttct
    28861 taacaattac aacagtcttt gcgtttttct tatcgtcaag aattacaaaa cctttaagac
    28921 gtttaagaga ccaagctaca cgtgtatctg aaggggatta ctcttataaa ccttctgtca
    28981 caacgaaaga tgaaattggt caattatcgc aggcatttaa tcagatgagt acagaaatcg
    29041 aagagcatgt cgacgcatta tccacatcta aaagtattag agacagctta attaactcta
    29101 tggtagaagg cgtcctaggt attaatgaga gtcgacaaat tatcttatct aataagatgg
    29161 cgaatgatat tatggacaat attgatgaag atgctaaagc tttcttatta agacaaatag
    29221 aagatacttt taaatcaaaa caaactgaaa tgcgcgattt agaaatgaat gcacgattct
    29281 ttgttgtgac tacaagctat atcgacaaga ttgaacaggg aggtaaaagt ggtgttgttg
    29341 tgacagttcg tgatatgact aatgagcaca atctagatca aatgaagaaa gatttcattg
    29401 ctaatgtatc acatgaatta cgtacaccga tatcattact tcaaggttat actgaatcaa
    29461 ttgtagatgg tattgttaca gaaccggatg aaataaaaga atcgcttgcc attgtccttg
    29521 atgaatcgaa acgtttaaat cgtttagtta atgaattgtt aaatgttgct cgcatggatg
    29581 ctgaagggtt atccgtaaat aaagaagttc agcctattgc agcgttacta gataagatga
    29641 aaattaagta tcgccaacaa gctgatgatt taggtctaaa tatgactttt aattattgta
    29701 agaagcgtgt ttggagttat gatatggatc gcatggacca agtactaacg aacttaattg
    29761 ataatgcatc acgttatacg aaacctggag atgaaattgc aattacttgt gatgaaaata
    29821 aaagcgaaga tattttatac attaaagata caggcacggg cattgcacca gaacatttac
    29881 aacaagtatt tgatcgtttt tataaagttg atgcagcgag aacgcgaggt aaacaaggta
    29941 ccggtttagg tttgttcatt tgtaaaatga ttatcgaaga gcatggtggt tccattgatg
    30001 ttaaaagcga attaggaaaa ggcacaacat ttattattaa actaccaaaa ccagaataaa
    30061 actgaatata gttatttaag aacgcatgtt attgattaga gattctaatt tatagcatgc
    30121 gttttttgat tgaagtgggg aattttgtaa gtgaattagg aaataggatt tatgattttt
    30181 gcgaataaca actattaaat atattactaa tttatacaaa aatata
//
LOCUS       DAHHOJ010000023        29033 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.023, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000023 DAHHOJ010000000
VERSION     DAHHOJ010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 29033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.023"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..758)
                     /locus_tag="NBH32_000861"
     CDS             complement(<1..758)
                     /locus_tag="NBH32_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HCD1514485.1"
                     /translation="MDLNLTMIIIIILFGFIAAFIDSVVGGGGLISTPALLAIGLPPS
                     VALGTNKLASSFGSLTSTIKFIRSGKVDLFVVAKLFGFVFLASACGAYIATMVPSQIL
                     KPLIIIALSSVFIFTLLKKDWGNTRTFTQFTFKKAIIFAALFILIGFYDGFVGGGTGS
                     FMLFVLLIFGFDFLSAAGNAKVLNFASNIGALVLFMVLGQVDYVIGLIMATSMIAGSY
                     TGAHFAIKQGVAYVKVLFIIITAILILKNAFDYI"
     gene            complement(802..2025)
                     /gene="thiI"
                     /locus_tag="NBH32_000862"
     CDS             complement(802..2025)
                     /gene="thiI"
                     /locus_tag="NBH32_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCD1514486.1"
                     /translation="MKYDHLLVRYGELTLKGSNRKKFVNQLRNNVNKSLKGLDGFVVK
                     GKRDRMYIELEDHADINEITYRLSKIFGIKSISPVLKVEKTIEAMSAAAIKFAQQFEE
                     NSTFKIDVKRADKNFPMDTYELQRELGGTVLKQIENVSVNVKRPDHEIRVEVRLDAIY
                     MYEEVVPGSGGLPVGTGGKTLLMLSGGIDSPVAGMEVMRRGVTIEAIHFHSPPFTSDQ
                     AKEKVIELTRILAERVGPIKLHIVPFTELQKQVNKVVHPRYTMTSTRRMMMRVADKLV
                     HQIGALAIVNGENLGQVASQTLHSMYAINNVTSTPVLRPLLTYDKEEIIIKSKEIGTF
                     ETSIQPFEDCCTIFTPKNPVTEPNFDKVVQYESVFDFEEMINRAVENIETLEITSDYK
                     TIKEQQTNQLINDFL"
     gene            complement(2025..3173)
                     /locus_tag="NBH32_000863"
     CDS             complement(2025..3173)
                     /locus_tag="NBH32_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1514487.1"
                     /translation="MFILIYLDNAATTKAFEEVLDTYLKVNQSMYYNPNSPHKAGLQA
                     NQLLQQAKAQINAMINSKTNYNVVFTSGATESNNLALKGIAYRKFDTAKEIITSVLEH
                     PSVLEVVRYLEAHEGFKVNYVDVKKDGSINLEHFKELMSDKVGLVTCMYVNNVTGQIQ
                     PIPQMAEVIKNYPKAHFHVDAVQAFGKISMDFNNVDSISLSGHKFNGLKGQGVLLVNH
                     IQNVEPTVHGGGQEYGVRSGTVNLPNDIAMVKAMKIANENFEALNAFVTELNNDVRQF
                     LNKYHGVYINSSTSGSPFVLNISFPGVKGEVLVNAFSKYDIMISTTSACSSKRNKLNE
                     VLAAMGLSDKSIEGSIRLSFGATTTKEDIARFKEIFIIIYEEIKELLK"
     gene            3323..3464
                     /locus_tag="NBH32_000864"
                     /pseudo
     CDS             3323..3464
                     /locus_tag="NBH32_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500331.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(3538..5232)
                     /gene="ezrA"
                     /locus_tag="NBH32_000865"
     CDS             complement(3538..5232)
                     /gene="ezrA"
                     /locus_tag="NBH32_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HCD1514488.1"
                     /translation="MVLYIILAIIVIILIAVGVLFYLRSNKRQIIEKAIERKNEIETL
                     PFDQNLAQLSKLNLKGETKTKYDAMKKDNVESTNKYLAPVEEKIHNAEALLDKFSFNA
                     AQSEIDDANELMDSYEQSYQQQLEDVNEIIALYKDNDELYDKCKVDYREMKRDVLANR
                     HQFGEAASLLETEIEKFEPRLEQYEVLKADGNYVQAHNHIAALNEQMKQLRSYMEEIP
                     ELIRETQKELPGQFQDLKYGCRDLKVEGYDLDHVKVDSTLQSLKTELSFVEPLISRLE
                     LEEANDKLANINDKLDDMYDLIEHEVKAKNDVEETKDIITDNLFKAKDMNYTLQTEIE
                     YVRENYYINESDAQSVRQFENEIQSLISVYDDILKEMSKSAVRYSEVQDNLQYLEDHV
                     TVINDKQEKLQNHLIQLREDEAEAEDNLLRVQSKKEEVYRRLLASNLTSVPERFIIMK
                     NEIDHEVRDVNEQFSERPIHVKQLKDKVSKIVIQMNTFEDEANDVLVNAVYAEKLIQY
                     GNRYRKDYSNVDKSLNEAERLFKNNRYKRAIEIAEQALESVEPGVTKHIEEEVIKQ"
     gene            5369..5833
                     /locus_tag="NBH32_000866"
     CDS             5369..5833
                     /locus_tag="NBH32_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HCD1514489.1"
                     /translation="MTTINPTNYALLKKQAASLIEDEHHMIAILSNMSALLNDNLDQI
                     NWVSFYLLEQNELILGPFQGHPACVHIPIGKGVCGTAVSERRTQIVADVHQFEGHIAC
                     DANSKSEIVVPIFKDDKIIGVLDIDAPITDRFDDNDKEHLEAIVKIIEKQLA"
     gene            6077..6679
                     /gene="rpsD"
                     /locus_tag="NBH32_000867"
     CDS             6077..6679
                     /gene="rpsD"
                     /locus_tag="NBH32_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019165370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCD1514490.1"
                     /translation="MARFRGSNWKKSRRLGISLSGTGKELEKRPYAPGQHGPNQRKKL
                     SEYGLQLREKQKLRYLYGMTERQFRNTFDIAGKKFGVHGENFMILLASRLDAVVYSLG
                     LARTRRQARQLVNHGHILVDGKRVDIPSYSVKPGQTISVREKSQKLNIIVESVEINNF
                     VPEYLNFDADSLTGTFVRLPERSELPAEINEQLIVEYYSR"
     gene            complement(6895..7638)
                     /locus_tag="NBH32_000868"
     CDS             complement(6895..7638)
                     /locus_tag="NBH32_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1514491.1"
                     /translation="MTLNKLKDELQIVSHRGLPSDFPENTMVGYREVMGLNVAMLEID
                     VHLTKDQHFVVIHDETIDRTSDGKGRIADYTLSQLKSFDFGSYKDVAFKGERIPTLDE
                     VLSLCLKYDKKLLIELKSPNLYPEIECKLLAFLEEKKVDATQVVIQSFDIECIEKLNT
                     LGSIYELGVLCSKRKYWYKKPNFSRISQIASYVNPNYALVTRKFVDEAHHHQLQVMPY
                     TVNKLKTGEKLRQMGVDGLITDNPKLFIK"
     gene            7718..8164
                     /locus_tag="NBH32_000869"
     CDS             7718..8164
                     /locus_tag="NBH32_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCD1514492.1"
                     /translation="MHQHDFKVQTSWQGGRNNVGNVQGDILSENISIPASLGGVGIGT
                     NPDEMLVSAASSCYIISLAATLERAKFTDISIEQQSIGTACLNNGKFSMSKIVHHPHI
                     EIPSDQIAQLEKRLPKLITIADNNCMISNAVRNNVDIKIYPIIQAK"
     gene            8279..9439
                     /locus_tag="NBH32_000870"
     CDS             8279..9439
                     /locus_tag="NBH32_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HCD1514493.1"
                     /translation="MYYHQPLLLTPGPTPVPDAIMREIQAPMVGHRSKDFEDIAQQAF
                     QGLKPIFGSQNDVLILTSSGTSVLEASMLNIVNPEDHFVVIVSGAFGNRFKQIAQTYY
                     KNVHIYDVTWGEAVDVKDFINYLSTLDVEVKAVFSQYCETSTTVLHPIHELGNAINQF
                     NSNIYFVVDGVSCIGAVDVDINKDKIDVLVSGSQKAIMLPPGLAFVAYSHRAKERFKE
                     VTTPKFYLDLNKYISSQADNSTPFTPNVSLFRGVNAYVETVKAEGFNHVIARHYAIRN
                     ALRSALKALDLTLLANDKDASPTVTAFKPNTNDEVKIIKDELKNRFKITIAGGQGHLK
                     GQILRIGHMGKISPFDILSVVSALEIILTEHRKVNYIGKGISKYMEVIHEAI"
     gene            9426..11030
                     /locus_tag="NBH32_000871"
     CDS             9426..11030
                     /locus_tag="NBH32_000871"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1514494.1"
                     /translation="MKQFNVLVADPISKDGIKALLDHEKFNVDIQTGLSEEALIKIIP
                     SYHALIVRSQTTVTENIINAADSLKVIARAGVGVDNINIDAATLKGILVINAPDGNTI
                     SATEHSLAMLLSMARNIPQAHQSLTNKEWNRNAFKGTELYHKTLGVIGAGRIGLGVAK
                     RAQSFGMKILAFDPYLTDEKAKSLSITKATVDEIAQHSDFVTLHTPLTPKTKGLINAD
                     FFAKAKPSLQIINVARGGIIDEKALIKALDEGQISRAAIDVFEHEPATDSPLVAHDKI
                     IVTPHLGASTVEAQEKVAISVSNEIIEILIDGTVTHAVNAPKMDLSNIDDTVKSFINL
                     SQTVGELAIQLMYNAPSSIKITYGGDLASIDSSLLTRTIITHILKDDLGPEVNIINAL
                     MLLNQQQVTLNIENNKAETGFSNYLEVELSNDSDSVKVGASVFTGFGQRIVRINNFSV
                     DLKPNQYQIVSYHNDTPGMVGKTGALLGKYNINIASMTLGRTEAGGDALMILSVDQPV
                     SNNIIDELKQVGEYNQIFTTELTVQS"
     gene            11124..11219
                     /locus_tag="NBH32_000872"
     CDS             11124..11219
                     /locus_tag="NBH32_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514495.1"
                     /translation="MKPLVQSFTSSCAVKQQSNYKLSSTPLSSPK"
     gene            complement(11180..12310)
                     /locus_tag="NBH32_000873"
     CDS             complement(11180..12310)
                     /locus_tag="NBH32_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1514496.1"
                     /translation="MKKILFDVDGVFLSEERCFDVSALTVYELLMDKCYLGLHSHIDW
                     ETLTDNDIQDIRNRIFQKDKILNKLKSLGLNSNWDMLFIVFSIHLIDILKKLSHDEIE
                     AFMYQDEPVELKLQNISTNLADCFNLNEQLPLQFLDNVKVGKNNIYAALEEFATTELH
                     VSDATLFSLKGALWTLAQEVYQEWYLGSKLYEDVEKKIARTTFKTGYIYQEIILRPVD
                     EVKVLLNDLKGAGFELGIATGRPYTETVVPFENLGLLPYFEADFIATASDVLEAENMY
                     PQARPLGKPNPFSYIAALYGNNRDKYESYINKQDNIVNKDDVFIVGDSLADLLSAQKI
                     GATFIGTLTGLKGKDAAGELEAHHADYVINHLGELRGVLDNL"
     gene            complement(12417..13883)
                     /locus_tag="NBH32_000874"
     CDS             complement(12417..13883)
                     /locus_tag="NBH32_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIBC"
                     /protein_id="HCD1514497.1"
                     /translation="MYKFFQNLGRSLMLPVAILPAAAIIAGIGNTLNALHATPKIAMF
                     FTTVGTTILEQLGILFAIGVAIGMAKKNDGAVALAATLGYFLVTVVLSPMKLAPLLGM
                     KASEINSAFEKMNNGNVFVGIVIGLIAAYAYNKFSETELPLALSFFSGKRLVPIMTAF
                     YCTFLVVILLFLWPLLYSWIVKFGESIVGLGSFGAFIYGVANRLLIPTGLHHALNSVF
                     WFDTIGINDIGKFQSGKDAIKGITGRYQAGFFPIMMFGIPAAALAMYHTAKTTQKKQV
                     YGWFLASSVAAFFVGVTEPIEFAFMFVAPILYVVHALLTGLSLFIAATFHWTAGFSFS
                     AGLIDYVLSLINPVSNHPLMLLVQGVVFFILYYVIFRVVIQVFNLNTIGRGENELVDP
                     TVVKDNIAPGENDIKQSKYHQHAIQILEGLGGQENIVNLTNCATRLRLELKDTSIIDQ
                     QKIKNAGAVGVTVNGKHSTQVIVGTHVQQVADEIEKHL"
     gene            complement(14011..14628)
                     /locus_tag="NBH32_000875"
     CDS             complement(14011..14628)
                     /locus_tag="NBH32_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HCD1514498.1"
                     /translation="MYSVISKILNFILVKMSKSLYVIGKDNIPKDSKYVVTCTHESYN
                     EVIMLGMALYPNQIHYMAKKELFKNKWIGKFLTSLNAFPVDRENPGPSTLKRPINLLK
                     DNKTVGIFPTGSRTSQEGAPLKRGASTIAMLSKSPILPVAYVGPTKIHGLLTGQAYIN
                     IGKPIDINDLPKDLKRNERIDYITKEIETRTAKLQQELHEIVKSL"
     gene            14800..16074
                     /locus_tag="NBH32_000876"
     CDS             14800..16074
                     /locus_tag="NBH32_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD1514499.1"
                     /translation="MSDFNHTDHSTTNHSQTPRYRRPKFPWFKTVIVALIAGIIGALL
                     VLGIGKVLNSTILNKDGSTVQTTNNKGGNQLDGQSKKFGTVHEMIKSVSPTIVGVINM
                     QKASSVDDLLKGKSSKPSEAGVGSGVIYQINNNSAYIVTNNHVIDGANEIRVQLHNKK
                     QVKAKLVGKDAVTDIAVLKIENTKGIKAIQFANSSKVQTGDSVFAMGNPLGLQFANSV
                     TSGIISASERTIDAETTGGNTKVSVLQTDAAINPGNSGGALVDINGNLVGINSMKIAA
                     TQVEGIGFAIPSNEVKVTIEQLVKHGKIDRPSIGIGLINLKDIPEEEREQLHTDREDG
                     IYVAKADSDIDLKKGDIITEIDGKKIKDDVDLRSYLYENKKPGESVTVTVIRDGKTKE
                     VKVKLKQQKEQPKRQSRSERQSPGQGDRDFFR"
     gene            complement(16168..17430)
                     /locus_tag="NBH32_000877"
     CDS             complement(16168..17430)
                     /locus_tag="NBH32_000877"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCD1514500.1"
                     /translation="MTNVLIEDLKWRGLIYQQTDEQGIEDLLNKEQVTLYCGADPTAD
                     SLHIGHLLPFLTLRRFQEHGHRPIVLIGGGTGMIGDPSGKSEERVLQTEEQVDKNIEG
                     ISKQMHNIFEFGTDHGAVLVNNRDWLGQISLISFLRDYGKHVGVNYMLGKDSIQSRLE
                     HGISYTEFTYTILQAIDFGHLNRELNCKIQVGGSDQWGNITSGIELMRRMYGQTDAYG
                     LTIPLVTKSDGKKFGKSESGAVWLDAEKTSPYEFYQFWINQSDEDVIKFLKYFTFLGK
                     EEVDRLEQSKNEAPHLREAQKTLAEEVTKFIHGEDALNDAIRISQALFSGDLKSLSAK
                     ELKDGFKDVPQVTLSNDTTNIVEVLIETGISPSKRQAREDVNNGAIYINGERQQDVNY
                     ALAPEDKIDGEFTIIRRGKKKYFMVNYQ"
     gene            17811..18716
                     /locus_tag="NBH32_000878"
     CDS             17811..18716
                     /locus_tag="NBH32_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1514501.1"
                     /translation="MTNQDNNHQLNHRIYHFEKIYKAIKHVIVFIFMIFIAIVAIAVI
                     AMSLYFHHLTKTSDSLSDDALIKKVRQIPGDELLDHNNKNLLYEYNHSQNSLIIGPKT
                     SSPNVIKALTSSEDTLFYKHDGILPKAILRAMIQDIFNTDQSSGGSTITQQLVKNQVL
                     TNEKTYSRKANELRLAIRLEHLLSKDEIIYTYLNIVPFGRDYNGANISGIASASYSLF
                     GIPPKDLSIAQSAYLIGLLQSPYGYTPYEKDGTLKSDKDLKYSIQRQHYVLKRMLIED
                     QITEKEYNDALKYDIKSHLLNRKKR"
     gene            complement(18696..18788)
                     /locus_tag="NBH32_000879"
     CDS             complement(18696..18788)
                     /locus_tag="NBH32_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514502.1"
                     /translation="MKIILLLFLIFGFIVVVTLKSEHQLTLFSI"
     gene            complement(18919..21606)
                     /gene="harA"
                     /locus_tag="NBH32_000880"
     CDS             complement(18919..21606)
                     /gene="harA"
                     /locus_tag="NBH32_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haptoglobin-binding heme uptake protein HarA"
                     /protein_id="HCD1514503.1"
                     /translation="MNKHHPKLRSFYSIRKSTLGVASVIVSTLFLITSQHQAQAAENT
                     NTSDKISENQNNNATTTQPPKDTNQTQPATQPANTAKNYPAADESLKDAIKDPALENK
                     EHDIGPREQVNFQLLDKNNETQYYHFFSIKDPADVYYTKKKAEVELDINTASTWKKFE
                     VYENNQKLPVRLVSYSPVPEDHAYIRFPVSDGTQELKIVSSTQIDDGEETNYDYTKLV
                     FAKPIYNDPSLVKSDKNDAVVTNDQSSSDASNQTNTNTSNQNTSTTNNTSDQPYATTN
                     MSQPAQPVSSANADQASSQPAHETNSNGNTNDKTNESSNQSDVNQQYPPADESLQDAI
                     KNPAIIDKEHTADNWRPIDFQMKNDKGERQFYHYASTVEPATVIFTKTGPIIELGLKT
                     ASTWKKFEVYEGDKKLPVELVSYDSDKDYAYIRFPVSNGTREVKIVSSIEYGENIHED
                     YDYTLMVFAQPITNNPDDYVDEETYNLQKLLAPYHKAKTLERQVYELEKLQEKLPEKY
                     KAEYKKKLDQTRVELADQVKSAVTEFENVTPTNDQLTDLQEAHFVVFESEENSESVMD
                     GFVEHPFYTATLNGQKYVVMKTKDDSYWKDLIVEGKRVTTVSKDPKNNSRTLIFPYIP
                     DKAVYNAIVKVVVANIGYEGQYHVRIINQDIKTKDDATSQNNTSEPLNVQTGQEGKVA
                     DTDVAENSSTATNPKDASDKADVIEPESDVVKDADNNIDKDVQHDVDHLSDMSDNNHF
                     DKYDLKEMDTQIAKDTDRNVDKDADNSVGMSSNVDTDKDSNKNKDKVIQLNHIADKNN
                     HTGKAAKLDVVKQNYNNTDKVTDKKTTEHLPNDIHKTVDKTVKTKEKAGTPSKENKLS
                     QSKMLPKTGETTSSQSWWGLYALLGMLALFIPKFRKESK"
     gene            21605..21694
                     /locus_tag="NBH32_000881"
     CDS             21605..21694
                     /locus_tag="NBH32_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514504.1"
                     /translation="MLLTTPIRLYLIDNNYQYQLLVLIVAHPN"
     gene            complement(21939..23606)
                     /locus_tag="NBH32_000882"
     CDS             complement(21939..23606)
                     /locus_tag="NBH32_000882"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HCD1514505.1"
                     /translation="MTHLSDLDIANQSTLQPIKDIAASVGISEDALEPYGHYKAKIDI
                     NKITPRENKGRVVLVTAMSPTPAGEGKSTVTVGLADAFHELNKNVMVALREPALGPTF
                     GIKGGATGGGYAQVLPMEDINLHFNGDFHAITTANNALSAFIDNHIHQGNELGIDQRR
                     IEWKRVLDMNDRALRHVNVGLGGPTNGVPREDGFNITVASEIMAILCLSRSIKDLKDK
                     ISRITIGYTRNRKPVTVADLKVQGALAMILKDAIKPNLVQSIEGTPALVHGGPFANIA
                     HGCNSILATETARDLADIVVTEAGFGSDLGAEKFMDIKAREAGFDPAAVVVVATIRAL
                     KMHGGVAKDNLKEENVEAVKAGIVNLERHVNNIKKFGVEPVVAINAFIHDTDAEVEYV
                     KSWAKENNVRIALTEVWEKGGKGGVDLANEVLEVIDQPNSFKPLYELELPLEQKIEKI
                     VTEIYGGSKVTFSSKAHKQLKQFKENGWDNYPVCMAKTQYSFSDDQTLLGAPSGFEIT
                     IRELEAKTGAGFIVALTGAIMTMPGLPKKPAALNMDVTDDGHAIGLF"
     gene            complement(24046..25752)
                     /gene="acsA"
                     /locus_tag="NBH32_000883"
     CDS             complement(24046..25752)
                     /gene="acsA"
                     /locus_tag="NBH32_000883"
                     /EC_number="6.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
                     /protein_id="HCD1514506.1"
                     /translation="MKVEVYKGAQGKHNLKDYEETYNTFNWKDVEQAFSWSETGKMNM
                     AYECIDRHVDQGIGDKIALNYKDEHRKESYTYKDMQRLSNKAANVLSEHAEVDKGDRV
                     FIFMSRTPELYFALLGVLKIGAIVGPLFEAFMEKAVVDRLENSEAKVLITNKALLPRV
                     PVDKLPNLKKIVVVDEDVEDNYIDFISLMETASDEFDIEWLKSDDGLILHYTSGSTGQ
                     PKGVLHVQQAMLVHYISGKYVLDLQEDDVYWCTADPGWVTGTSYGIFAPWLNGATNCI
                     AGGRFSPEQWYSMIEDFKVTIWYTAPTALRMLMSAGDDIVEKYDLSSLRSILSVGEPL
                     NPEVIKWAKKVYGLTVLDTWWMTETGGHMIVNYPTMDVKLGSMGKPLPGIQAAIIDDA
                     GNELPPNRMGNLAIKKGWPSMMYRIWKNPEKYKSYFIGDWYVSGDSAYKDEDGYFWFQ
                     GRVDDVIMTAGERVGPFEVESKLVEHEAVAEAGIIGKPDPVRGEIIKAFVALRKGYEP
                     TDELKEEIRLFVKEGLSAHAAPREIEFKDKLPKTRSGKIMRRVLKAWELNLDAGDLST
                     ME"
     gene            25921..26553
                     /locus_tag="NBH32_000884"
     CDS             25921..26553
                     /locus_tag="NBH32_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1514507.1"
                     /translation="MNHLKTYQSEDYYIHDKQFVIEGPLTYEDLKALTFDAHLTAFRD
                     AEDQYEALLEITTLPEGRIYVARQDQLIVGYVTFHYPDEIERWSTGNLPYLIELGAIE
                     VSINFRQLQLAEKLIQLSLSTPEFEDYIVITTEYYWHWDLKNSKLDVFDYKKLMQRLM
                     ATGGLEIFATDDPEITSHPANCLMARIGKNITLEQQQAFDDIRYMNRFFF"
     gene            26578..27747
                     /locus_tag="NBH32_000885"
     CDS             26578..27747
                     /locus_tag="NBH32_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoin utilization protein AcuC"
                     /protein_id="HCD1514508.1"
                     /translation="MQQHSSKTAYVYSDKLLQYRFHDQHPFNQMRLKLTTELLLNANL
                     LSPEQIVQPRIATDDELMLIHKYDYVEAIKHASHGIISEDEAKKYGLNDEENGQFKHM
                     HRHSATIVGGALTLADLIMSGKVLNGCHLGGGLHHAQPGRASGFCIYNDIAITAQYLA
                     KEYNQRVLIIDTDAHHGDGTQWSFYADNHVTTYSIHETGKFLFPGSGHYTERGEDIGY
                     GHTVNVPLEPYTEDASFLECFKLTVEPVIKSFKPDIILSVNGVDIHYRDPLTHLNCTL
                     HSLYEIPYFVKYLADSYTNGKVIMFGGGGYNIWRVVPRAWSHVFLSLIDQPIQSGYLP
                     LEWINKWKHYSSELLPKRWEDRLNDYTYVPRTKEISEKNKKLALHIASWYESTRQ"
     gene            complement(27845..28834)
                     /gene="ccpA"
                     /locus_tag="NBH32_000886"
     CDS             complement(27845..28834)
                     /gene="ccpA"
                     /locus_tag="NBH32_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HCD1514509.1"
                     /translation="MTVTIYDVAREARVSMATVSRVVNGNQNVKAETKNKVNEVIKRL
                     NYRPNAVARGLASKKTTTVGVIIPDISNIYYSQLARGLEDIATMYKYHSIISNSDNDP
                     EKEKEIFNNLLSKQVDGIIFLGGTITEEMKELINQSSVPVVVSGTNGKDAHIASVNID
                     FTEAAKEITGELIEKGAKSFALVGGEHSKKAQEDVLEGLTEVLNKNGLQLGDTLNCSG
                     AESYKEGVKAFAKMKGNLPDAILCISDEEAIGIMHSAMDAGIKVPEELQIISFNNTRL
                     VEMVRPQLSSVIQPLYDIGAVGMRLLTKYMNDEKIEEPNVVLPHRIEYRGTTK"
     gene            complement(28907..29020)
                     /locus_tag="NBH32_000887"
     CDS             complement(28907..29020)
                     /locus_tag="NBH32_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514510.1"
                     /translation="MKKVYIKLKYIVRYHLAFVFISSFSLNFSKQKYVTVN"
BASE COUNT         9442 a         5305 c         4215 g        10071 t
ORIGIN      
        1 tgaatataat caaaagcatt ttttaaaatt aaaatagcag taataataat aaataatact
       61 tttacatagg caacaccttg tttgatagca aaatgtgctc cagtatatga cccagcaatc
      121 atgcttgtag ccataattaa acctattaca taatctactt gtcctaatac cataaataat
      181 acaagcgcac ctatattaga agcaaagttc aaaactttag catttcctgc agcacttaaa
      241 aaatcaaaac caaagattaa caatacaaaa agcataaatg aacctgttcc cccacctaca
      301 aatccatcat aaaagccgat taatataaaa agtgctgcaa atattatggc tttcttaaat
      361 gtaaattgag taaacgtacg tgtattgccc caatcttttt taagtaatgt gaatataaac
      421 accgacgaaa gtgcaatgat gattaaaggt ttcaatattt gtgacggaac catcgttgca
      481 atatatgcgc cacatgcaga tgccaaaaat acaaaaccaa ataatttggc aacaacaaat
      541 aagtccactt taccggacct tataaacttt atcgtactag ttaaagaacc aaatgaactt
      601 gccaatttat ttgtacctaa agccacagat ggtggtagac cgattgctaa taatgctggc
      661 gtagaaatta aaccgccacc ccctacaacc gaatctataa acgccgcgat aaaaccaaat
      721 aaaattatga ttataatcat cgttaagttc aaatccatag tttaacatcc ccttatgttt
      781 aaatttactc taggatttta tttataaaaa gtcgtttatt aattggtttg tttgctgttc
      841 tttaatagtt ttataatcac tagttatttc aagtgtttca atattttcaa cagcacgatt
      901 aatcatctct tcaaaatcaa agacgctttc atattgaact accttatcaa agtttggttc
      961 ggttactgga tttttagggg tgaaaattgt acaacaatct tcaaatggtt gaatagatgt
     1021 ttcaaatgtg ccaatttctt tcgatttaat aataatttct tctttatcgt aagttaataa
     1081 aggacgtaat acaggagtag aagttacatt attaattgca tacatgctat gaagtgtttg
     1141 actggctacc tgccctaggt tttcaccatt tacaatagct aaagccccta tttgatgtac
     1201 taatttatca gcaacacgca tcatcatacg tctcgttgaa gtcattgtat atcttggatg
     1261 tacaacttta tttacctgtt tttgcaattc tgtaaatggt acaatatgca atttaattgg
     1321 tccaacacgt tcagctaaaa tacgtgtcaa ttcaataact ttttcttttg cttgatcact
     1381 tgtaaatggt ggactatgga aatgaatcgc ttcaattgtt acgccacgtc tcatcacttc
     1441 cattccagca actggtgagt ctataccgcc tgaaagcatt agtaacgtct taccaccagt
     1501 accaactggt aatccgcctg aacccggaac aacttcttca tacatataaa ttgcatctaa
     1561 tctaacttcc actcgaattt catgatctgg acgtttgaca ttaacagaaa cattttcaat
     1621 ttgtttcaat acggtaccac ccaattcacg ttgtaattca tacgtatcca ttgggaaatt
     1681 tttatcggca cgcttcacat caattttaaa tgtgctgttt tcttcaaatt gctgcgcaaa
     1741 tttaattgcc gctgcactca ttgcctctat tgttttttct acttttaata ctggactaat
     1801 agatttaata ccgaaaattt ttgataatcg atatgttatt tcatttatat ctgcatggtc
     1861 ttcaagttca atatacatac gatctcgttt gcctttaacg acaaacccat caagtccttt
     1921 taatgactta tttacattat ttcttaattg atttacaaat ttctttctat ttgaaccctt
     1981 taatgttaac tccccgtatc taacaagcaa gtgatcatac ttcattattt tagcaactcc
     2041 ttaatttcct cataaatgat gataaatatt tctttaaacc ttgctatatc ttctttagtt
     2101 gtagtagccc caaatgataa tcttatacta ccttcaatag atttgtctga taatcccatt
     2161 gcagccaata cttcatttaa tttattacgt ttagatgaac aagcactcgt cgtagatatc
     2221 ataatgtcat attttgaaaa agcattaact aatacttcac cttttactcc aggaaaacta
     2281 atatttaaaa cgaatggtga acctgaagtt gaagagttaa tataaactcc atgatattta
     2341 tttaaaaatt gacggacgtc attatttaac tcagtaacaa atgcattcaa tgcttcaaag
     2401 ttttcattag ctatcttcat cgctttaacc attgcaatat catttggcaa attaactgtt
     2461 ccacttctaa cgccatattc ttgaccacca ccatggacag ttggttcaac attttgaatg
     2521 tgatttacaa gtaagacgcc ttggcctttt aaaccattaa acttgtgtcc acttaaacta
     2581 atactatcta cgttattgaa atccattgaa attttgccga atgcttgaac cgcatctaca
     2641 tgaaaatgtg ccttaggata attttttata acttcagcca tttgtggaat aggctgtatt
     2701 tgtcctgtta cattatttac atacatacat gttactaaac cgactttgtc tgacattaat
     2761 tctttgaagt gttctaagtt aatactgcca tctttcttta catcaacata attaacttta
     2821 aatccttcgt gcgcttccaa atatcttaca acctctaata cggatggatg ctctaacacg
     2881 gatgtaatta tttccttagc tgtatcaaat ttacgatagg caataccttt taaagcaaga
     2941 ttattggatt cagttgcacc actcgtgaaa acaacattat aatttgtttt tgaattaatc
     3001 attgcattaa tttgggcttt tgcttgctgt agtaattgat ttgcctgcaa accagcttta
     3061 tgcggactat tcggattata atacattgat tgatttactt ttaaataagt atctaacact
     3121 tcttcaaatg ctttcgtcgt tgccgcatta tctagatata tcaaaataaa cacttctctt
     3181 tcattaatca tcaatcgtta aaattgtttt actcaagcat tatactatta tccgacaaaa
     3241 aataaaggtt atagcgcaaa ataatcttat ttctacttat taaataagaa ttagactatt
     3301 tcactctata ttaaacagct gtgtgattat gcttttttca ctacttatat taattaaaac
     3361 aacacttttg attacgttta tctttaaaca attgctattt atatttattg tccctttcat
     3421 atatccatat aaaaaaagaa gctagacaac atacagttgg ctaacttcaa ttattaaact
     3481 catcagtcca atttgacaga gagctcatat tatttaagta taactacata ctagtttcta
     3541 ttgcttaata acttcttctt caatatgttt agtgacacct ggctcaacac tttcaagagc
     3601 ttgctctgca atttcaatcg cacgcttata gcgattattt ttaaataatc gttcagcttc
     3661 atttaagctc ttatcaacat tgctatagtc cttacgatat ctatttccat attgaattaa
     3721 tttctctgca taaacagcat taacaagaac atcatttgct tcatcttcaa atgtattcat
     3781 ttgaatcaca attttagaca ctttatcttt taactgttta acgtgtattg gacgttcact
     3841 aaattgttcg ttaacatcac gaacttcatg atcaatttca tttttcatga tgataaatct
     3901 ttcaggaaca cttgttaagt tagaagcaag taatcgacga tacacttctt ctttcttcga
     3961 ttgtactcgt aacagattgt cttctgcttc tgcttcatct tcacgtaatt gaatcagatg
     4021 attttgtagc ttttcttgtt tgtcattaat gactgtgaca tgatcttcta aatattgtaa
     4081 attatcctga acctcgctat atctaacagc agatttagac atttctttta aaatatcatc
     4141 atatacagaa attaaacttt gaatttcatt ttcaaattga cgaacacttt gagcatcaga
     4201 ttcatttata tagtagtttt cacgtacata ttcaatttct gtttgcaatg tataattcat
     4261 gtctttagct ttgaataagt tatccgtaat gatatctttt gtttcttcga catcattttt
     4321 agctttaact tcatgttcaa ttaaatcata catgtcatct aacttatcat tgatattagc
     4381 tagtttatca ttagcttctt ctaattctaa gcgactaatt aatggttcaa cgaaactaag
     4441 ctctgttttt aagctttgta atgtactgtc tacttttacg tgatccagat catacccttc
     4501 aactttaaga tcacggcaac catattttaa atcttggaat tgaccaggta attctttttg
     4561 agtttctcta attaattctg gtatttcttc catataagat cttagctgtt tcatttgttc
     4621 attcaaggca gctatatggt tgtgcgcttg tacataatta ccatcagctt ttagtacttc
     4681 atattgctct aaccttggct caaatttttc aatttcagtt tcaagtagac ttgctgcctc
     4741 accaaattga tgacgatttg ctaaaacatc acgtttcatt tcacgataat caaccttaca
     4801 tttgtcatat aattcatcat tatctttgta taacgcaata atttcattta catcttctaa
     4861 ttgttgctga tagctttgtt cgtaactatc catcaactca tttgcatcat caatttcact
     4921 ttgagctgcg ttgaaactaa atttatctaa taaagcctca gcattatgga ttttttcttc
     4981 cacaggagct agatacttat ttgtactttc tacgttgtcc tttttcattg catcgtattt
     5041 cgtttttgtt tcacctttta aattcaactt agataattgt gcaaggtttt gatcaaaagg
     5101 taacgtctca atttcatttt tacgttcgat tgctttttca ataatttgtc gtttatttga
     5161 acgtaaatag aataatacac ctacagcaat caatataatc acaattattg ccaaaatgat
     5221 atataacacc atatgcttct cctcctaatt tatcattact attataacgt aattttgctt
     5281 aaggataaat tataaaatga atttattgcc ataatatttt cgaaattcta tactattata
     5341 aaatctatga aaataagagg ttttaaatat gacaacaatt aacccaacaa actatgcatt
     5401 attaaagaaa caagcagcaa gccttattga agatgaacat cacatgattg ctattttaag
     5461 taacatgtct gccttattaa atgataatct ggatcaaatt aattgggtca gcttttactt
     5521 attggaacaa aatgaactta tacttggacc tttccaagga caccccgctt gtgtccacat
     5581 tccaattgga aaaggtgtat gtggtacagc cgtttcagaa cgtcgtacac aaattgtagc
     5641 tgatgttcat caattcgaag gacatatcgc ttgtgatgct aatagtaagt ctgagattgt
     5701 cgttccaatt ttcaaagatg ataaaattat cggcgtctta gatatcgatg cccctataac
     5761 ggatcgattt gatgacaatg acaaagaaca tcttgaagca attgttaaaa ttattgaaaa
     5821 gcaactcgca taaaaggaca tcagcatttt caataaagtg ttgacagtta gcaggaaaat
     5881 gttacaataa tctttgtgtg aattaacgaa agtagcagtt gtatattatt gagcgctatg
     5941 ttgttcccaa tgcggacgtg tcacgtaact gtcgctataa ggtgaagaca cataaaacaa
     6001 tatatcttag taagcatgca acactctttt ttgtttattc ataacaacaa aaaagaatta
     6061 aaggaggagt cttattatgg ctcgattcag aggttcaaac tggaaaaaat ctcgtcgttt
     6121 aggtatctct ttaagcggta ctggtaaaga attagaaaaa cgtccttacg caccaggaca
     6181 acatggtcca aaccaacgta aaaaattatc agaatatggt ttacaattac gtgaaaaaca
     6241 aaaattacgt tacttatatg gaatgactga aagacaattc cgtaacacat ttgacatcgc
     6301 tggtaaaaaa ttcggtgtac acggtgaaaa cttcatgatc ttattagcaa gtcgtttaga
     6361 cgctgttgtt tattcattag gtttagctcg tactcgtcgt caagcacgtc aattagttaa
     6421 ccacggtcat atcttagtag atggtaaacg tgttgatatt ccatcttatt ctgtaaaacc
     6481 tggtcaaaca atttcagttc gtgaaaaatc tcaaaaatta aacatcatcg ttgaatcagt
     6541 tgaaatcaac aatttcgtac ctgagtactt aaactttgat gctgacagct taactggtac
     6601 tttcgtacgt ttaccagaac gtagcgaatt acctgctgaa attaacgaac aattaatcgt
     6661 tgagtactac tcaagataat accgttcaat acaaacaccc acaattgtgg gtgttttttt
     6721 attaatttaa aaatgataga ttcaagatta tatcgagcta ccctatttta caatcaactt
     6781 agaatttaca agtcttatat gttgaattat ctataatatt tcctcattag tttttaaaat
     6841 tatcaattgc gatatacgaa tcctcatccc gctatcaaat aaccattcat cgtctcactt
     6901 aataaataat ttcgggttat ctgtaattaa tccatctaca cccatttgtc ttaatttttc
     6961 tccagttttc aatttattaa ctgtatatgg cataacctgt aattgatgat gatgtgcttc
     7021 atcaacaaat tttctggtca ctaatgcata attaggatta acataactag caatttgaga
     7081 aattcttgaa aagtttggct ttttatacca atacttacgt ttactacaaa gcacacctaa
     7141 ttcatatata ctgcccaatg tgtttaactt ttcgatgcat tcgatatcaa aggattgtat
     7201 aactacttgt gtcgcatcga ctttcttttc ttccaaaaat gccagcaatt tacattctat
     7261 ttctggatat aaattcggac ttttcaattc aatcaatagc tttttatcat actttaagca
     7321 taatgaaagt acttcatcta acgtgggtat tcgttctccc ttaaaagcaa catctttata
     7381 actaccaaaa tcaaatgatt ttaattgcga taatgtgtaa tcagcaatac gccccttacc
     7441 atccgatgtt ctatcaattg tttcatcatg tatcacaaca aaatgttggt ctttggtcaa
     7501 atgaacatct atttctaaca tagcaacatt gagccccatt acctctcgat aaccgaccat
     7561 tgtattttca ggaaaatcac tcggtaatcc acggtgcgaa acaatttgta attcatcttt
     7621 cagtttattc agagtcattg ataagtcacc tttctgttaa actttgataa actattctaa
     7681 aattaaacta tcacaattat attggaggtt aaagcaattg catcaacatg actttaaagt
     7741 ccaaacttct tggcaaggtg gtcgtaacaa tgtcggaaac gttcaaggcg acatactttc
     7801 agagaatatt tcaatacctg cttctttagg tggtgttggt ataggaacaa atcccgatga
     7861 aatgttagta tcagccgctt catcatgtta tatcatctca ttagcagcta ctcttgaacg
     7921 tgcaaagttc acagatattt caattgaaca acaatcgatt ggaacagctt gtttaaataa
     7981 tggaaaattc agtatgtcaa aaattgtgca ccatcctcac attgaaattc caagtgatca
     8041 aatagcacaa ttagaaaagc gattaccaaa attgataaca attgcagata ataattgcat
     8101 gatttcaaat gctgtaagaa ataatgtaga cataaaaatc tatcccatca ttcaggccaa
     8161 ataaaatcgt tacgtgataa cgtttattcg acaatatagc gttatcactt tttttaggct
     8221 tttaaatttt cagaaaattt gctaaaatag tgaaatctta tttataggag gcatataaat
     8281 gtattatcat caaccgttgt tattaacacc tggcccaacc cctgtacctg atgccattat
     8341 gagagaaatt caagcaccta tggttggtca tcgttctaaa gattttgaag acatcgcaca
     8401 acaagcattt caaggtctaa agccaatatt tgggagtcaa aatgatgtac ttattttaac
     8461 atctagcggt acaagcgtct tagaagctag tatgttgaac attgtaaacc ctgaagatca
     8521 cttcgttgtc attgtttcag gtgcctttgg taaccgattt aaacaaattg cacaaactta
     8581 ttacaaaaat gtgcacattt atgacgtaac atggggagaa gctgtagatg ttaaagattt
     8641 catcaattac ctttcaactt tagatgttga agttaaagca gtatttagtc aatattgcga
     8701 aacatctacg acagtgctac accctattca cgagttagga aatgccatca atcaatttaa
     8761 tagtaatatt tattttgtag ttgacggcgt aagttgcatt ggggctgttg atgttgacat
     8821 taacaaagat aaaattgatg tccttgtttc tggtagtcaa aaagcaatta tgttacctcc
     8881 aggattagct tttgtagctt atagccaccg cgcaaaagaa cgtttcaaag aagtaactac
     8941 accaaaattt tatctagact taaataaata catttcgtca caagctgaca attctacacc
     9001 gttcacacca aatgtgtctt tatttagagg tgtaaatgca tacgttgaaa ccgtaaaagc
     9061 agaaggtttc aatcacgtaa tagcacgaca ctatgcaatt agaaatgcat taagaagcgc
     9121 cttaaaagca ttagatttaa ctttattagc caatgataaa gatgcatctc caacggttac
     9181 agcattcaaa cctaatacaa atgatgaagt gaaaataatc aaagatgaac ttaaaaatcg
     9241 ctttaaaata acaattgctg gtggtcaagg ccatcttaaa ggtcaaattt taagaattgg
     9301 tcatatgggg aaaattagtc ctttcgatat tttatcggta gtatctgctt tagaaattat
     9361 tttaactgaa caccgtaaag ttaactatat cggtaaaggt atatcaaaat atatggaggt
     9421 tattcatgaa gcaatttaat gtactcgttg cagatcccat atcaaaagat ggtatcaaag
     9481 cattattaga tcacgaaaaa tttaatgtag atattcaaac tggcttgtcc gaagaagcat
     9541 taatcaaaat tataccttca taccatgctt taatcgttcg tagtcaaact acggtcactg
     9601 aaaatatcat aaatgctgct gattctttaa aagtaatcgc acgcgccggt gttggtgtag
     9661 ataatattaa tattgatgct gcaacattaa aaggtatttt agttattaat gccccagatg
     9721 gtaatacgat ttcagctact gaacattcac tggcaatgtt attatcaatg gctcgaaata
     9781 ttccgcaagc acaccaatca cttacaaata aagaatggaa tcgaaatgca tttaaaggta
     9841 ctgagcttta tcataaaaca ttaggtgtca ttggtgctgg tagaattggt ttaggtgttg
     9901 ctaaacgtgc gcaaagtttc ggaatgaaaa tactagcttt tgacccttac ttaacggatg
     9961 aaaaagcaaa atctttaagc attacgaagg caacagttga tgagattgcc caacattctg
    10021 atttcgttac attacataca ccactaacac ctaaaacaaa aggcttaatt aatgctgact
    10081 tttttgccaa agcaaaacct agtttgcaaa taatcaatgt ggcacgtggt ggtattattg
    10141 atgaaaaggc gctaataaaa gcattagacg aaggacaaat tagtcgggca gctatcgatg
    10201 tgtttgaaca tgaacctgca actgattcgc ctcttgttgc acatgataaa attattgtta
    10261 cacctcattt gggtgcttca acagtcgaag ctcaagaaaa agtggcaatt tctgtttcaa
    10321 atgaaatcat cgaaatttta attgatggta ctgtaacgca tgcagtgaat gcacctaaaa
    10381 tggacttaag caatatagat gatactgtaa aatcattcat caatttaagc caaacagttg
    10441 gtgaattagc tattcaatta atgtacaatg caccaagctc tattaaaatt acgtacggtg
    10501 gcgacttagc ctctattgat agtagtttat taacacgtac aattattact catattttaa
    10561 aagatgatct tggtcctgaa gtcaatatta tcaatgctct aatgttgtta aaccaacaac
    10621 aagtgacatt aaatattgaa aataataaag cagagacagg ttttagtaac tacttagagg
    10681 tagaactatc aaacgatagc gattccgtta aagttggcgc ttctgtcttt acaggtttcg
    10741 gtcaaagaat tgttagaatt aataattttt ctgtagactt aaagccaaat caatatcaaa
    10801 ttgtgtcata tcataatgat actccaggta tggtaggaaa aactggcgca ttgttaggta
    10861 aatacaatat caacattgca tctatgactt taggtagaac tgaagcgggc ggagatgcgc
    10921 taatgatttt atccgttgat caacctgttt caaacaatat aattgatgaa cttaaacaag
    10981 ttggtgaata caatcaaatt ttcacaactg aattgacggt acagtcataa acaatttatg
    11041 taaattcaaa atgccaagaa gacattggat tacttccatt acttcttggc attttttcat
    11101 tattaatttc attgtaactc aatatgaaac ctttggttca atcattcact tcaagttgtg
    11161 ctgtcaaaca acaatcaaat tacaaattat ctagtacacc tctaagttca cctaaatgat
    11221 taataacata gtcggcatga tgcgcttcta actcacctgc agcatcctta ccttttaaac
    11281 ctgttaatgt tccaataaac gttgcaccta ttttttgagc acttaataag tcagctaacg
    11341 aatcgcctac tataaatacg tcatctttat ttacaatgtt atcttgctta ttgatataag
    11401 attcatattt atcgcgatta ttaccatata aagctgcgat ataactaaaa ggattcggct
    11461 ttcctaatgg tcgtgcttgc ggatacatat tctctgcttc taaaacatca cttgcagttg
    11521 caataaaatc agcttcaaaa tatggtaaca atcctaaatt ttcaaatggc acaacagtct
    11581 cagtataagg acgacctgtt gcaataccta attcgaaacc agcacctttt aaatcattca
    11641 gaagtacctt aacttcatct actggtctca aaataatttc ttgataaata taacctgtct
    11701 taaaagtagt tcgtgctatt ttcttttcaa catcttcata caacttcgat cctaaatacc
    11761 attcttgata aacttcttgc gctaacgtcc ataatgcacc cttcaaacta aataaagtag
    11821 catccgaaac atgtaactct gttgttgcaa actcttccaa agcagcataa atattatttt
    11881 taccaacttt tacattatcc aaaaattgta aaggtagttg ttcatttaaa ttaaaacagt
    11941 ctgctaagtt tgtgcttata ttttgcaact tcaattcgac aggctcatct tgatacatga
    12001 atgcctcaat ttcatcatgt gataactttt tcaaaatatc aattaaatga atactaaaaa
    12061 cgataaataa catatcccaa tttgaattta gccctagcga ttttaatttg tttaaaatct
    12121 tatctttttg aaaaattcga ttcctaatgt cttgtatatc gttatcagtc aaagtttccc
    12181 aatctatatg tgaatgaaga cctaaataac acttatccat taataattca tataccgtta
    12241 atgcagagac atcgaaacaa cgttcttcac ttaaaaaaac gccatcaaca tcaaataaaa
    12301 ttttcttcac aatccccact ccatttctga aaattcagat ataaatcatt ctactatttg
    12361 actaaaaaaa gcgcaaaccc tattgaagtt ggatttgcgc tttagctgtt taaattttat
    12421 aaatgttttt caatttcatc agcaacctgc tgtacgtgtg taccgacaat aacttgagtt
    12481 gaatgtttac cattaacagt aacaccaact gcaccggcgt ttttaatctt ctgttgatca
    12541 ataatagatg tgtcttttaa ctctagacgc aaccttgttg cacaattagt taaattaaca
    12601 atattctctt gaccacctaa accttctaat atttgtatag catgttgatg atatttactt
    12661 tgtttaatat cattttcacc aggagcaata ttatctttta caactgttgg atcaactaat
    12721 tcattttcac ctctaccaat cgtattcaag ttaaatactt ggattactac acggaaaatc
    12781 acatagtata agatgaaaaa tacaacacct tgaacaagca acatcaatgg atgatttgat
    12841 actggattaa ttagtgataa cacataatct atcaaacctg cactaaatga aaatccagct
    12901 gtccaatgga atgtagctgc gataaataaa gataatcctg ttaataacgc atgaacaaca
    12961 tataagattg gcgcaacaaa cataaatgca aactcaatcg gctctgtaac accaacgaaa
    13021 aatgctgcaa ctgaactcgc taggaaccaa ccgtaaactt gttttttctg agtcgtttta
    13081 gctgtatgat acattgctaa cgcagccgct ggaataccga acatcatgat tgggaagaat
    13141 cccgcttgat agcgtcctgt aatacctttt atagcatctt tgccactttg gaatttacca
    13201 atatcattaa taccaatcgt atcaaaccag aacacactat tcagtgcatg atgtaatcct
    13261 gtaggaatta ataatctatt ggcaacacca tatatgaaag ctccaaacga tcctaaaccg
    13321 actatagatt caccaaattt tacaatccat gaataaagta gtggccataa gaataacaat
    13381 atgacaacta aaaatgtaca gtaaaatgca gtcataattg gaactagacg tttaccacta
    13441 aaaaatgata atgctaatgg taattctgtt tcactaaact tattgtatgc ataagctgct
    13501 attaaaccta ttacaatacc aacaaagaca ttgccattat tcatcttttc aaaagctgaa
    13561 tttatttccg aagctttcat tcctaataaa ggcgctaatt tcattggtga taatacaact
    13621 gtaactaaaa aatatcctaa cgtagctgca agcgcgactg caccatcatt tttctttgcc
    13681 attcctatag ctacaccaat tgcaaataaa atacctaatt gctctaaaat cgtagtacct
    13741 accgtagtaa agaacattgc gattttcggc gtcgcatgaa gtgcatttaa cgtattacca
    13801 attccggcaa taattgctgc agccggtaaa atggcaactg gtaacattaa cgaacgccct
    13861 aaattttgga aaaatttata cattgaatgt catccttctt aaaataaggt agaaatataa
    13921 agattactaa tgtaactaga ataactactt cgatactccg ttatagtcac ctaggcttac
    13981 taaccagcta tatttctacc tcaagttatt ttataaactt tttacaattt catgcaattc
    14041 ttgttgtaac tttgctgttc gtgtttcaat ctcttttgta atataatcga tacgctcgtt
    14101 tcgttttaaa tctttaggta aatcgttaat atcgattggt ttaccaatat ttatgtatgc
    14161 ttgtcctgtt aaaagaccgt gaatcttagt aggaccaaca taagcaacag gtaatattgg
    14221 tgacttactt aacattgcaa ttgttgaagc accacgtttc aaaggtgcac cttcttgcga
    14281 tgtgcgagaa cctgttggga agataccaac tgtcttatta tctttcaaca aattgattgg
    14341 gcgttttaaa gtactaggtc ctggattttc acgatctaca ggaaatgcat ttaaagacgt
    14401 taaaaattta ccaatccatt tatttttgaa taattctttt ttagccatat aatgaatttg
    14461 attaggatat aatgccatac ctagcataat gacttcgtta taactttcat gcgtacaagt
    14521 tacgacatat ttactatcct taggaatatt atctttaccg attacgtata atgattttga
    14581 cattttaact aaaatgaaat tcaaaatctt actaatcact gaatacattg tgccacctac
    14641 ttaacttatt tgtttataac aactagattt tatcactata tttttactag aacaagatag
    14701 tctgaaaata catctaaagt tgtatagtgt attactagca ataattataa aaaaaagaca
    14761 taattaaagc atatataaat catataatgg aggttaagta tgtcagattt taatcataca
    14821 gatcattcta caacaaacca tagccaaaca cctagataca gaagacctaa atttccatgg
    14881 tttaaaacag tcatcgttgc attgattgct ggaattattg gtgcacttct agtacttggt
    14941 ataggcaaag tattaaatag tacaatttta aataaagatg gttcaactgt ccagacaaca
    15001 aataataaag gtggcaatca attagacggt caaagcaaga aattcggtac cgttcatgaa
    15061 atgataaaat ctgtctcccc tacaattgtt ggagttatta acatgcaaaa agcatcaagt
    15121 gtagacgact tattaaaagg caaatcatct aaaccatctg aagctggagt aggttcaggt
    15181 gttatctatc aaataaacaa caattcagct tatatcgtta caaacaatca tgttattgat
    15241 ggcgcaaatg aaattagagt ccaattacat aataaaaaac aagttaaagc gaaattagtt
    15301 ggtaaagatg cagtaactga tattgctgta cttaaaattg aaaatacaaa aggtattaaa
    15361 gcgattcaat ttgccaactc ttcaaaagta caaactggcg atagcgtatt cgcaatgggt
    15421 aacccattag gattacaatt tgctaactct gtaacatctg gtatcatttc agcaagcgaa
    15481 cgtacgattg acgctgagac aactggtggc aatacaaaag tcagcgttct tcaaacagat
    15541 gctgctatta acccaggtaa ctcaggtggc gcattagtag atattaatgg taatttagtt
    15601 ggtattaact caatgaaaat tgctgcgaca caagttgaag gtatcgggtt tgctattcca
    15661 agtaatgaag ttaaagtaac aattgaacaa cttgtaaaac atggtaaaat tgaccgccct
    15721 tcgattggta ttggtttaat taatttgaaa gatattcctg aagaagagcg cgagcaactt
    15781 catactgata gagaagacgg tatttatgtc gccaaagctg atagtgatat tgatcttaaa
    15841 aaaggtgata ttattacaga aattgatggc aagaaaatta aagatgatgt tgatttaaga
    15901 agctatttat atgaaaataa aaaacctggt gaatcagtca ctgttaccgt tatccgtgat
    15961 ggtaaaacaa aagaagttaa agtgaaatta aaacaacaaa aagaacaacc aaaacgtcaa
    16021 agccgatcag aacgtcaatc acctggccaa ggcgatagag atttctttag ataatttaca
    16081 gacagtaaag tacattaaga gcctactcat attcattcct aagaatgtaa tgagtaggct
    16141 ctaatttatt tagctatgca attatattta ttgatagtta accatgaagt attttttctt
    16201 accgcgacga ataatcgtaa attcgccatc aattttatct tctggtgcta aagcataatt
    16261 aacatcttgt tgtctctcac cattaatata aatcgcacca ttgttaacat cttcacgtgc
    16321 ttgtcgttta gaaggagaaa tgcctgtttc aataaggact tcaacgatat ttgttgtgtc
    16381 atttgataat gtcacttgag gcacatcttt aaatccatct tttaattctt tcgctgataa
    16441 tgattttaaa tcaccactaa ataatgcttg tgaaatacgg attgcatcat ttaatgcatc
    16501 ttcaccatga ataaatttag ttacttcttc agctaatgtt ttttgagctt cacgtaaatg
    16561 cggtgcttca tttttagatt gttctaagcg atcaacttct tcttttccta agaaagtaaa
    16621 gtattttaag aatttaatta catcttcgtc tgattgatta atccagaatt gataaaattc
    16681 ataaggactt gttttttcag catctaacca aacagcacct gactcagact taccaaattt
    16741 cttaccatct gatttagtta caagcggaat agttaaaccg tatgcgtctg tttgaccata
    16801 catacgacgc attaattcaa taccacttgt gatattaccc cattgatctg atccacctac
    16861 ttgaatctta caattcaatt ctctattcaa atgaccgaaa tcaatagctt gtaaaatcgt
    16921 gtatgtgaat tctgtatatg aaataccatg ttctaaacga ctttggattg aatctttacc
    16981 taacatgtaa ttaacgccga cgtgtttacc atagtcacgt aaaaagctaa ttaatgagat
    17041 ttgtcctaac cagtctctat tattaacaag cactgcacca tggtctgttc caaattcaaa
    17101 aatattgtgc atttgcttac taataccttc gatattttta tctacttgtt cttctgtttg
    17161 tagcacacgt tcttctgatt tacctgatgg atcaccaatc atacctgtac caccgccaat
    17221 taaaacgata ggacgatgtc catgttcttg aaatcttctt aatgttaaaa atggtaataa
    17281 gtgaccaatg tgtaagctat ctgctgttgg atcggcacca cagtataacg tcacttgttc
    17341 tttatttaat aaatcttcaa taccttgttc atcagtttgt tgataaataa gacctctcca
    17401 ttttaaatct tcaattaata cattcgtcat tattatttcc tcctatttat aattatttat
    17461 tgaatacttg ttaaaatact ttaaagcttt ttgaacgtaa aaaaaccctt acaacaaata
    17521 tgtaagggcg cgattgcacg ttaccaccaa acttaaacat aatcataaga taatgttcac
    17581 tctattaatg atacgttcat taataaacgt aggacatgtt agttataaag gtgtattcat
    17641 attattaaaa acactagttc acagcgacca ctagctctct gatgatttca aataatatta
    17701 cttgtccttt tatcctattc tttatgcatt ttccctaatt ataattaacg ttaaaataaa
    17761 agtcaaattg cttaaatatg gtatactata cgtaatttag gaggttaaag atgacgaatc
    17821 aagacaacaa tcatcaattg aatcatcgta tatatcattt tgaaaagata tataaagcta
    17881 tcaaacatgt cattgttttt atatttatga ttttcattgc catcgttgct atcgctgtga
    17941 ttgcgatgtc tttatatttt catcatttaa ctaaaacgtc cgactcatta tcagatgatg
    18001 ctttaataaa aaaagttcga caaatacctg gcgatgaatt attagatcat aataacaaaa
    18061 atttattata tgagtataac cattctcaaa actcactcat tataggccct aaaacatcaa
    18121 gtccaaatgt cattaaagca ttaacgtcat ctgaagacac tttattttat aaacatgatg
    18181 gcatcttacc aaaggcgatt ttaagagcaa tgatacaaga tatttttaat actgatcaaa
    18241 gttcaggtgg tagcacaatt acacaacaac ttgttaaaaa tcaagttctt accaacgaaa
    18301 aaacatatag tagaaaagca aatgaacttc gcctagcaat tagattagaa cacctactct
    18361 caaaagatga aattatatat acatatttaa atatagttcc cttcggtaga gattataatg
    18421 gcgctaatat ttccggaatt gcatccgctt catatagttt gtttggtatt ccaccaaaag
    18481 atttatcaat tgcacaatct gcatacctta tcggtttgtt gcaaagccct tatggctata
    18541 caccctacga aaaagatgga acgttaaaat cggataaaga tttgaaatat agtattcaaa
    18601 gacaacatta tgtattaaag cgtatgttaa tcgaagatca aatcactgaa aaagaataca
    18661 acgacgcatt aaaatatgat attaaatcac atttgttaaa tcgaaaaaag cgttaattga
    18721 tgctcacttt ttaaagtaac cacaacaatg aatccaaata ttaaaaacag cagtaagatt
    18781 attttcaatt agaaaatttc tcactgctgt tctttttatt tactctaaag gaaagccatt
    18841 aaaccaatca atagtttata aattaattgc tttcacattg tctacaaagt taatcataca
    18901 tatgctatat ccagttaatt atttagattc ttttctgaat ttaggaatga ataaagctaa
    18961 catacctaat aacgcatata agccccacca tgattggctt gaagttgttt ctccagtttt
    19021 tggtagcatt ttagattgac taagtttgtt ttctttcgat ggtgtgccgg ccttttcttt
    19081 tgttttcact gttttatcta cagttttatg aatatcattc ggcagatgtt cagttgtttt
    19141 tttgtcagta actttgtctg tattattata attttgtttc actacgtcaa gctttgctgc
    19201 ttttccagta tgattatttt tatcggcaat atgattcagc tgtatgactt tgtctttatt
    19261 tttattagag tctttatcag tatcgacatt cgatgacata ccaacgctat tatcggcatc
    19321 tttatccaca tttctatcag tatctttggc aatttgagta tccatttctt ttaaatcata
    19381 cttatcgaag tgattattat ccgacatatc ggataaatga tcaacatcat gttgcacatc
    19441 tttatcaata ttattatcag catctttaac cacgtcagac tctggttcta tcacatctgc
    19501 tttatcagac gcatctttag gatttgttgc agtgctgcta ttttcagcta catctgtatc
    19561 agcaacctta ccttcttgtc ctgtttgtac atttagcggt tcactcgtgt tattttgtga
    19621 tgtagcatca tcttttgtct tgatatcctg atttataatt ctgacatgat attgaccttc
    19681 ataaccaatg tttgccacaa cgactttaac aatcgcattg taaactgctt tgtcaggtat
    19741 atatgggaaa atcagcgttc tagaattatt tttaggatct ttagaaacag tagtgacacg
    19801 tttaccttct acaattaaat ctttccagta actgtcatcc tttgttttca tcactacata
    19861 tttttgacca tttaaagttg ctgtatagaa tggatgttca acaaagccgt ccataactga
    19921 ctcactattt tcttcacttt caaaaacaac aaaatgcgct tcttgtaaat ctgttaattg
    19981 atcatttgta ggtgtaacat tttcaaattc cgtcactgct gatttaacct gatcagctaa
    20041 ctctactcta gtttgatcta atttcttttt atattccgcc ttatattttt ccggcaattt
    20101 ctcttgtaat ttttctaatt cataaacttg tctttctaac gttttagctt tgtgatacgg
    20161 agctaataat ttttgtaaat tgtatgtttc ctcatccaca tagtcgtctg ggttattagt
    20221 aataggctgt gcaaagacca ttagcgtata atcatagtct tcatggatgt tctcaccata
    20281 ttcaatagat gacacaattt taacttctct cgtaccatta gatactggga aacgaatata
    20341 tgcataatct ttatcagaat catatgatac taattcgact ggtaactttt tgtcaccttc
    20401 ataaacttca aatttcttcc atgttgaagc tgtctttaaa cctaattcaa ttattggtcc
    20461 tgtttttgta aaaatgacag ttgctggttc aacagtacta gcataatgat agaactgtct
    20521 ttcaccttta tcatttttca tttgaaaatc aattggtcgc caattatcag ctgtatgttc
    20581 tttatcgatg atagccgggt ttttaattgc atcttgtagt gattcatctg ctggtggata
    20641 ctgttgatta acatccgact gattacttga ctcattcgtt ttatcgttag tattaccatt
    20701 agaatttgtt tcatgagctg gttgacttga tgcttgatct gcattggctg acgatactgg
    20761 ttgtgcaggt tgactcatat tggtcgttgc gtacggttga tcactagtat tgttcgtcgt
    20821 tgatgtattt tgattagatg tattcgtgtt tgtttgatta cttgcgtctg aacttgattg
    20881 atcattcgtt actactgcat catttttatc tgattttaca agtgaaggat cgttataaat
    20941 aggtttagca aatactaatt tagtataatc ataatttgtt tcttctccat catcaatttg
    21001 agtcgaagaa acaattttca attcttgtgt gccatctgaa actgggaatc gaatataggc
    21061 atggtcttct ggtacaggac tatatgatac aagtctcact ggcaattttt gattgttttc
    21121 atagacttca aactttttcc atgttgaagc agtattgatg tctaattcaa cttctgcttt
    21181 ctttttagtg taatacacat ctgctggatc tttgatgctg aaaaagtgat agtactgcgt
    21241 ttcattgttt ttatctaata actggaaatt gacttgttct cttggaccta tatcatgttc
    21301 tttattttct aatgcaggat ctttaattgc atctttaagt gattcatccg ctgcaggata
    21361 gtttttcgca gtgtttgctg gttgcgtagc aggttgtgtt tgatttgtat cctttggtgg
    21421 ctgagttgta gttgcattat tattttgatt ttccgagatt ttatctgaag tatttgtatt
    21481 ttctgctgct tgcgcttgat gttgagaagt aattaaaaat agtgtactga caatgaccga
    21541 tgcaacgcct agagttgatt ttctaataga atagaaagac cttaattttg ggtgatgttt
    21601 gttcatgttg ttaacaactc ctataagatt atatttaatt gataataatt atcaatatca
    21661 attattagtg ttaattgtag cgcatcccaa ttaaatattg caacctattt tattaaaatt
    21721 ttttaatatg aatattcatt gcatcgcttt cctattcttc aagcccactt tttatcttca
    21781 tatacattaa ttaaccacct tcaaacattg tcgttagatt cgccaaatgg aatctatttt
    21841 tagcacagca aaaaaccgaa ctgctgaaat aatgcatctc caacagtccg gtctattaaa
    21901 ctatttactt atctttatta aacaattgac atgatttatt agaataaccc aattgcatga
    21961 ccatcatcag taacatccat gtttaatgct gctggttttt taggtaaacc aggcatagtc
    22021 atgattgcac ctgtcaacgc tacgataaat cctgcacctg ttttcgcttc taattcacga
    22081 attgtaattt caaatcctga tggtgcacct aacaacgttt gatcatctga gaatgaatat
    22141 tgtgttttcg ccatacatac tgggtaatta tcccaaccat tttctttaaa ttgttttaat
    22201 tgtttatgcg ctttactgct aaacgttact tttgaaccgc catagatttc agtcacaatc
    22261 ttttcaatct tttgctctaa tggtaattct aattcatata aaggtttaaa tgaattaggt
    22321 tgatcaatga cttctaatac ttcatttgct aagtcaacgc cacctttacc acctttttcc
    22381 caaacttcag ttaaggcaat tcgtacgtta ttttctttag cccaagattt tacatattct
    22441 acttctgcat cggtatcatg tataaatgca ttaattgcaa caaccggttc tacaccgaat
    22501 tttttaatat tattaacatg acgctctaaa ttaacaattc ctgcttttac tgcttctaca
    22561 ttttcttctt ttaaattatc tttcgctaca ccaccatgca tttttaatgc acgaattgtc
    22621 gcaacaacaa cgacagctgc cggatcaaat cctgcttcac gcgctttaat atccatgaat
    22681 ttttcagcgc ctaagtctga accaaatcca gcttccgtta caacgatatc agctaaatca
    22741 cgtgctgttt cagttgctaa aattgagtta caaccgtgtg cgatattcgc aaatggtcca
    22801 ccatgaacta atgctggtgt cccttcaatt gattgtacta agtttggttt aattgcatct
    22861 tttaaaatca ttgcaagtgc accttgcact tttaaatctg caactgtaac tggcttgcga
    22921 tttctagtgt aaccaatagt aatacgacta attttatctt ttaagtcttt aatacttcta
    22981 cttaaacata aaatcgccat aatttcagac gctactgtaa tattaaagcc atcttcacgt
    23041 ggtacaccat ttgtaggtcc acctaaccca acgtttacat gtctaagtgc acgatcattc
    23101 atatctaata cacgtttcca ttcaatacgt ctttgatcga ttcctaattc attaccttgg
    23161 tgaatatgat tatcgataaa cgcagacaat gcattatttg cagttgtaat cgcatggaaa
    23221 tctccgttga aatgtaagtt gatatcttcc ataggtaaga cttgcgcata accaccacca
    23281 gtcgcaccac ctttgatacc aaatgttggt cctaaagcag gctctcttaa tgcaaccata
    23341 acgtttttat ttaactcatg gaatgcatca gctaaaccaa ctgtaaccgt tgatttacct
    23401 tcaccagctg gtgttgggct catcgcagtt actaaaacaa ctctcccttt gttttctctt
    23461 ggcgtaattt tattaatgtc gattttagct ttgtaatgac cataaggttc taatgcatcc
    23521 tctgaaatac ctactgatgc agcaatatcc ttaattggtt gtagtgttga ttgattcgca
    23581 atatctaaat ctgataaatg agtcaacttt tttcgcccct ttttgtttaa tttatgcatt
    23641 tataatatgt aagttgtagc taagaaattt ttacttacat tcatataata acgaactgtt
    23701 aaaaacttgt cgactaaatc gggttagcgc tttcattttt gcaataaatt cacttattaa
    23761 agttttgtat tatacaactt ttagcgcttg atactattga taactatatt tttctattat
    23821 ttaaaatagt catcttccaa agatcatatt aaaaataaga agcaatataa agatatgcca
    23881 ttattgttat gtcatcattt tcaaatgcat tctacgaaat gatatgacac aagttaatag
    23941 ctctttatat tgcttatcat aaagtaaact aatatttaaa cattgacatg aaatatgttg
    24001 tcacccttta ttcttagaaa gaaatcttct ataacattca tgtcattatt ccattgtact
    24061 taaatcccct gcatctaaat ttaattccca agcttttaat acacgtctca taattttacc
    24121 tgaacgtgtt ttaggtaatt tatctttaaa ttcgatttca cgtggtgctg catgcgccga
    24181 caaaccttct ttaacaaata aacgaatttc ttcttttaat tcgtctgttg gttcatatcc
    24241 tttcctcagt gcaacaaacg ccttaattat ttcaccgcga accggatcag gtttaccaat
    24301 aattcctgct tcggcaactg cttcgtgttc aaccaattta gactcaacct caaatggtcc
    24361 aactcgttcg ccagctgtca taattacatc atcaacacgt ccttggaacc agaaataacc
    24421 atcttcatct ttatatgccg agtcaccaga tacataccag tctccaataa aatacgactt
    24481 atatttttct ggattcttcc agatacgata catcattgat ggccagcctt tttttatagc
    24541 aagattgccc attcgatttg gtggtaattc attacctgca tcatcgataa ttgcagcttg
    24601 aataccaggt aatggtttgc ccattgagcc aagcttaacg tccatcgttg gatagttaac
    24661 aatcatatgt ccacctgttt ctgtcatcca ccaagtatct aacaccgtta aaccgtatac
    24721 ttttttcgcc cattttataa cttcaggatt taaaggctca cctactgaca gaatcgaacg
    24781 taacgatgac aagtcatatt tctcaacaat atcgtcacca gcactcatta acattcttaa
    24841 agctgttggt gccgtatacc aaatcgtcac tttaaaatct tcaatcatac tataccactg
    24901 ttctggtgaa aagcgaccac cagctataca atttgtagcg ccatttaacc atggtgcaaa
    24961 aataccataa gatgttcctg taacccaacc tggatctgct gtacaccaat aaacatcatc
    25021 ttcttgtaaa tctaatacat attttccaga aatatagtgc actaacatcg cttgttgaac
    25081 atgcaataca cctttaggtt gcccagtaga acctgatgta taatgtaaaa tcaaaccatc
    25141 atccgacttt aaccattcaa tgtcaaattc atcgctagca gtttccatca aactaatgaa
    25201 gtctatgtaa ttgtcttcta catcctcatc tacgacaaca atttttttca agttcggtaa
    25261 tttatctaca ggtactcgag gtaacaatgc cttattagta attaacactt tagcttcact
    25321 gttctctaat ctatccacaa ctgccttttc cataaatgct tcaaataacg gaccaacaat
    25381 tgcaccaatt tttaaaacac ctaacaacgc aaaatatagt tcaggtgtac gcgacataaa
    25441 tataaatact ctgtcacctt tgtcaacttc tgcatgttca gacaaaacat tcgctgcttt
    25501 attagataac cgttgcatat ccttataagt atacgattct tttctgtgct catctttgta
    25561 atttaatgct attttatccc ctattccttg atctacatgg cgatctatac attcatatgc
    25621 catgttcatt tttccagttt cactccaaga aaatgcttgt tctacgtctt tccaattaaa
    25681 agtattatat gtttcttcat aatctttaag gttatgtttg ccttgcgctc ctttataaac
    25741 ttcgactttc atttgaaact ccccctttgt tatgtgaaaa cgctttcttt ctcaatatta
    25801 tacataacat ttctaattat tcaaaatttt tacttttatc ctttttctag tattaattat
    25861 tgtaaatttt ctatataatg aatttaatac aatcacgacg tcgattgagg agttgaaaat
    25921 atgaatcatt taaagacgta tcaatccgaa gattattaca ttcatgacaa gcaatttgtt
    25981 attgaaggac ctttaacata cgaagatttg aaagcgctta ctttcgatgc gcatttaacc
    26041 gcatttagag atgctgaaga tcagtatgaa gctttgttag aaattacaac attaccagaa
    26101 ggtagaattt atgttgctcg ccaagatcaa ctcattgtgg gttatgtcac tttccactat
    26161 cctgatgaaa ttgagcgctg gtctacaggt aaccttccat atttaatcga attgggggca
    26221 attgaagtca gcatcaattt taggcaatta caacttgcag aaaagctgat acaacttagc
    26281 ctttctacac cagaattcga ggattatatc gttataacta cagaatatta ctggcattgg
    26341 gatttaaaaa attcaaagtt agatgtattt gactataaaa aattaatgca gcggttaatg
    26401 gcaactggtg gacttgaaat attcgctaca gatgatccag aaataacaag tcatccagct
    26461 aattgtttaa tggcaagaat tggcaaaaac attacattag aacagcaaca agcgtttgat
    26521 gatattcgtt atatgaatcg gtttttcttt taatttttgg aatatcggag ggattatatg
    26581 caacaacatt catcaaaaac tgcatatgtt tattcagata agttattaca atatcgattt
    26641 catgaccaac atcccttcaa tcaaatgcgt ttaaaattaa caacagagct acttttgaat
    26701 gcaaatttat tgtctcctga acaaatagta caacctagaa ttgcaacaga tgacgaatta
    26761 atgttaattc ataaatatga ttacgtcgaa gctattaagc atgcttcaca tggcattatc
    26821 agtgaagatg aggctaagaa atatggatta aatgatgaag agaatggtca atttaagcat
    26881 atgcaccgcc atagcgccac aattgttgga ggcgctttaa ctttagcaga tcttattatg
    26941 tcaggcaaag tattaaatgg ttgtcactta ggtggtggat tgcatcacgc tcaacctggt
    27001 cgagcaagtg gtttttgtat atacaatgat attgcaatta ccgcacaata cttagctaaa
    27061 gaatacaatc aacgcgtttt aatcatagat accgatgcac atcatggaga tggtacacaa
    27121 tggagtttct atgccgataa ccatgttact acttattcta tccatgaaac tggaaaattt
    27181 cttttcccag gctctggtca ctatactgag cgcggtgaag atatcggcta tggacacact
    27241 gtaaatgtcc cacttgaacc gtatacagaa gatgcatcat ttttggagtg ttttaaatta
    27301 acagttgagc ctgtcataaa gagttttaaa cctgatatta ttctaagcgt aaatggtgtc
    27361 gatatacatt atcgtgatcc actaactcat ctaaattgta cgttacattc attatatgaa
    27421 attccatatt ttgtaaaata tttagctgat tcttatacga atggaaaggt aattatgttt
    27481 ggtggcggag gctacaatat ttggagagtc gtaccacgtg catggagtca tgtattctta
    27541 agtttaattg atcaaccaat tcaaagtggt tatttaccgt tagaatggat taataaatgg
    27601 aaacattatt catctgaatt attacctaaa agatgggaag atcgtttaaa tgattatacc
    27661 tatgtccctc gcacaaaaga aattagtgaa aaaaataaaa aattagcttt acatatagcg
    27721 agttggtacg aatctactcg tcaataagta acctaatctg caacgatttt aaaagataaa
    27781 taaaaggcat tcttccaaca cccatataat tcgggtcgtt agatgaatgc ctaattttgt
    27841 gaatttattt tgtagttcct cggtattcaa ttctgtgagg taaaactaca tttggttctt
    27901 ctatcttttc atcattcata tattttgtta ataagcgcat ccctactgca ccgatatcat
    27961 ataatggttg aataacacta gaaagttgtg gtctaaccat ctcaactaaa cgtgtattat
    28021 tgaaactaat aatttgtaat tcctctggaa ctttaatacc agcatccatt gcactatgca
    28081 taataccaat agcttcttcg tcactaatac ataatatagc atctggaaga ttacctttca
    28141 ttttggcaaa agcttttacg ccttctttat aactttcagc accagaacaa ttcaatgtat
    28201 cacctaattg aagaccattt ttatttaaca cttcagttaa accttctaaa acatcttctt
    28261 gagctttttt agaatgttct ccacctacta aagcaaatga tttagcgcct ttttcaatta
    28321 attctcccgt aatttctttc gcagcttcag taaaatcaat attaactgat gctatatgtg
    28381 catccttacc atttgttcct gatactacta caggtacaga tgattgattt atcaattctt
    28441 tcatttcttc agtaattgta ccaccaagga aaataatacc atcaacttgt ttacttaata
    28501 agttattaaa aatttctttt tccttttcag gatcgttatc tgaatttgaa ataattgagt
    28561 gatatttata cattgttgca atatcttcaa gtccacgagc aagttgtgaa taatagatat
    28621 tagatatatc tggaatgatc acacctactg ttgttgtctt tttactagct aaacctctag
    28681 caacagcatt tggacgataa ttcaaacgct taatgacttc gttaacttta tttttagttt
    28741 ctgctttaac attttggttc ccattaacaa cacgcgacac tgtggccata gagacacgcg
    28801 cttctcttgc tacatcatat atagtaactg tcataatttc ctccttgtaa acgttttatt
    28861 cattataata acatgttttc atttcatttt catagtttac catattttaa tttacagtaa
    28921 catatttttg tttggaaaaa tttagcgaaa agcttgaaat aaaaacaaac gctaaatgat
    28981 aacgaacgat atattttaat tttatataaa cttttttcat ttttaattgt aat
//
LOCUS       DAHHOJ010000024        28695 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.024, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000024 DAHHOJ010000000
VERSION     DAHHOJ010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 28695)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28695)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28695
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.024"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            19..1239
                     /locus_tag="NBH32_000888"
     CDS             19..1239
                     /locus_tag="NBH32_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic family protein"
                     /protein_id="HCD1514511.1"
                     /translation="MGYRTLKSIFHEHNESKMKDEYTKRFNSLASFNTNINIIPMENG
                     KKVNDLEYPLFFMVTKNLSKKQELISINSRKIDRALNSLPYAAREQYFNDLLIDELQS
                     TNEIENVFSTKQEIAHALNNQASEFLKFRGLVDQYKEIELNKKIKVDNVRDIRAIYDK
                     LVSNEINEQDKLDGELFRKNFVGVHDGSTNKYIHVGLQPETKIVEYIGEMLTFLKYFD
                     APQPFKIMASHYLFEYIHPFYDGNGRVGRFIIAKLLSDYYDNYTALTFSYVINRNKSK
                     YYKAFMTASNHLNCGDLTEFIDTMLELLIAGQERILDELIPKMDATEKLTLYLTSHYK
                     QIDYEFLYLLSMDKLFGNKRNRLTLIDLENILGVGRVKINNTIKKFGNYLVKIKSRPT
                     IYEISDEFLNSIIK"
     gene            1842..2594
                     /locus_tag="NBH32_000889"
     CDS             1842..2594
                     /locus_tag="NBH32_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000503564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exotoxin"
                     /protein_id="HCD1514512.1"
                     /translation="MGEFEVKYLTGFILILLLEGIFTNSASAIEYSDLHHKSELDSKR
                     LYNAKVSFANPTQLENKKTNDRLLKHDLLFHDMFVNDAWKKDFKVEFENEALSKKFVN
                     KDIDIFAGNYGYGCHGGETNKTLCSYGGVTLSDNNKYDDYKNIPCNLWIDGHQTGIEL
                     TAVKTKKKIVTIQELDVQLRNYLNEKYKLYELGGDIVKGYVKYHNDDEKNVEYDFYNL
                     NGEYGHEVLKMYADNKTINSDKLHLDIYLFKS"
     gene            complement(2796..3065)
                     /locus_tag="NBH32_000890"
     CDS             complement(2796..3065)
                     /locus_tag="NBH32_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514513.1"
                     /translation="MAENNQNSLVTKIATYGSFIAIASFVILFISIFLKFSFNIEFTA
                     TIMNICRYTLILGFILMSLPDVVDKNIKKIIFDAFIIIVFIFFLL"
     gene            3379..4065
                     /gene="mtnN"
                     /locus_tag="NBH32_000891"
     CDS             3379..4065
                     /gene="mtnN"
                     /locus_tag="NBH32_000891"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCD1514514.1"
                     /translation="MIGIIGAMEEEITILKNKLTQLSEISVAHVKFYTGILKDREVVI
                     TQSGIGKVNAAISTTLLINKFKPDIIINTGSAGALDESLNVGDVLISDDVKYHDADAT
                     AFGYEYGQVPQMPVAFQSSKPLIDKVSQVVQQQQLTAKVGLIVSGDSFIGSVEQRQKI
                     KKAFPNAMAVEMEATAIAQTCYQFNVPFVVVRAVSDLANGEAEMSFEAFLEKAAVSSS
                     QTVEALVSQL"
     gene            4085..4612
                     /locus_tag="NBH32_000892"
     CDS             4085..4612
                     /locus_tag="NBH32_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HCD1514515.1"
                     /translation="MGLVRKFFMPNSYVQSIFQIDLDKLVDKGVKGIITDLDNTLVGW
                     DVKEPTERVKAWFKEANEKGITITIVSNNNESRVASFSQHLDIDFIFKARKPMGKAFD
                     KAITKMNIRPDQTVVIGDQMLTDVFGGNRRGLYTIMVVPVKRTDGFITKFNRLIERRL
                     LRHFSKKGYITWEEN"
     gene            4613..5713
                     /gene="yqeH"
                     /locus_tag="NBH32_000893"
     CDS             4613..5713
                     /gene="yqeH"
                     /locus_tag="NBH32_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HCD1514516.1"
                     /translation="MSDILKCIGCGAPLQSEDKNKPGFVPEHNMFRDDVICRRCFRLK
                     NYNEVQDVGLESEDFLKLLSGLADKKGIVVNVVDVFDFEGSFINAVKRIVGNKKIILA
                     ANKLDLLPKQINKRRVKEWLKRTARKYGLEADDVVLISAEKGWGIDDLLSSIANIREN
                     EDVYIVGTTNVGKSTLINKLIEASVGEKDVVTTSRFPGTTLDMIDIPLDETSFMYDTP
                     GIIQDHQMTHLVSEKELKIIMPKKEIKQRVYQLNEAQTLFFGGLARIDYVSGGKRPLV
                     CFFSNDLNIHRTKTEKANDLWRNQLGDLLTPPGNPQNFDLNEVKAVRLETGKEKRDVM
                     ISGLGFITIGPGAKVIVRVPKNVDVVLRNSIL"
     gene            5727..6533
                     /gene="aroE"
                     /locus_tag="NBH32_000894"
     CDS             5727..6533
                     /gene="aroE"
                     /locus_tag="NBH32_000894"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCD1514517.1"
                     /translation="MKFAVIGNPISHSLSPVMHRANFNSLGLDDTYEALNIPIEDFHL
                     IKEIISKKELDGFNITIPHKERIIPYLDHVDEQAINAGAVNTVLIKDGKWIGYNTDGI
                     GYVKGLHSVYPDLENAYILILGAGGASKGIAYELAKFVKPKLTVANRTMARFESWNLN
                     INQISIADAEKYLAEFDIVINTTPAGMAGNNESIINLKHLSPNTLMSDIVYIPYKTPI
                     LEEAERKGNHIYNGLDMFVYQGAESFKIWTNKDADINSMKTAVLQQLKGE"
     gene            6537..6827
                     /gene="yhbY"
                     /locus_tag="NBH32_000895"
     CDS             6537..6827
                     /gene="yhbY"
                     /locus_tag="NBH32_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCD1514518.1"
                     /translation="MLTGKQKRYLRSLAHNIDPIFQIGKGGINENMIKQIDDTLENRE
                     LIKVHVLQNNFDDKKELAETLSEATRSELVQVIGSMIVIYRESKENKEIELP"
     gene            6830..7399
                     /gene="nadD"
                     /locus_tag="NBH32_000896"
     CDS             6830..7399
                     /gene="nadD"
                     /locus_tag="NBH32_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HCD1514519.1"
                     /translation="MKKIVLYGGQFNPIHTAHMIVASEVFHELQPDEFYFLPSFMSPL
                     KKHNNFIDVQHRLTMIQMIIDELGFGDICDDEIKRGGQSYTYDTIKAFKEQHKDSELY
                     FVIGTDQYNQLEKWYQIEYLKEMVTFVVVNRDKNSQNVENAMIAIQIPRVDISSTMIR
                     QRVSEGKSIQVLVPKSVENYIKGEGLYEH"
     gene            7389..7973
                     /gene="yqeK"
                     /locus_tag="NBH32_000897"
     CDS             7389..7973
                     /gene="yqeK"
                     /locus_tag="NBH32_000897"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HCD1514520.1"
                     /translation="MNIEKAKRLAKEKLPEKRYNHSLRVAETAIKLAEIYDGDTSKVE
                     LAGVLHDFCKYDDLGKMYQIVRQYELGNDLLSYGSEILHGPVCAAIMEHEYGINDEEV
                     LMAIKYHTTGRQQMTKTEKLIFIADYIEPGRTIPGVDDIRDMAYNQGSLDKTIYEISK
                     RTVLFLIQKDITVYNKTIDCLNYYNYSDERIKDD"
     gene            7974..8327
                     /gene="rsfS"
                     /locus_tag="NBH32_000898"
     CDS             7974..8327
                     /gene="rsfS"
                     /locus_tag="NBH32_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCD1514521.1"
                     /translation="MNSQELLAIAVDAIDNKKGEDTISLEMKGISDMTDYFVVTHGNN
                     ERQVQAIARAVKEVANEQNIEVKRMEGYNEARWILIDLADVVVHVFHKDERNYYNIEK
                     LYQDAPLESYSQVAY"
     gene            8330..9046
                     /locus_tag="NBH32_000899"
     CDS             8330..9046
                     /locus_tag="NBH32_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1514522.1"
                     /translation="MSQYAEMSLVYDQLTQDQPYEKWFEIVKNHCKDESNILDIGCGT
                     GSLTVQLEALGNVTGMDLSVDMLTVAAQKSANVNWLEGDMTSFDLQQQFDIITIFCDS
                     LNYLQDETAVIETFINVYHHLTDSGVFIFDVHTVYKMMNLFNNQSYIDDKGDIFLAWD
                     AVQGDLPLSVYHDMTFFIRHEDETYSRFDESHFQRTFDEKTYLSWLAQVGFKQVETFT
                     DFNIDEHNEDAERLFFIAKK"
     gene            9086..9772
                     /locus_tag="NBH32_000900"
     CDS             9086..9772
                     /locus_tag="NBH32_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001802022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEA family DNA-binding protein"
                     /protein_id="HCD1514523.1"
                     /translation="MVLLYQFLLRYKDFLTQWKLYIISAVVLIMVLIGFIFWRQDDYT
                     SRNFENKDTALKQSTSENSSLSKLEDVQVKDGDNSKNKGPVYVDVKGAVKHPNVYKMT
                     SKDRVVDLLDKAQLLEDADVSQINLSEKLTDQKMIFIPHKGQKNVEPQIGVNSVHVKN
                     GNTNNTKVNLNTASVSELMSVPGVGQAKANAIVEYRNQQGAFQEIDDLKKVKGFGSKT
                     FDKLKSYFTI"
     gene            9864..10325
                     /locus_tag="NBH32_000901"
     CDS             9864..10325
                     /locus_tag="NBH32_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000439692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HCD1514524.1"
                     /translation="MERIKWEEYFMAQSHLLALRSTCQRLSVGATIVKDNRIIAGGYN
                     GSVAGEVHCIDEGCLIEDGHCIRTIHAEMNALLQCAKQGVSTEGATIYVTHFPCLNCT
                     KSIIQAGIKRIYYAEDYHNHEYATKLLKQSGIEFKKIPFSPEYVAKYLTKG"
     gene            10330..12532
                     /locus_tag="NBH32_000902"
                     /pseudo
     CDS             10330..12532
                     /locus_tag="NBH32_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837660.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
     gene            12589..13563
                     /gene="holA"
                     /locus_tag="NBH32_000903"
     CDS             12589..13563
                     /gene="holA"
                     /locus_tag="NBH32_000903"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCD1514525.1"
                     /translation="MSDNIVAIYGDVPELVEKQSAEIISQFLKSDRDDFNFVKYNLYE
                     TEIAPIVEETLTLPFFSDKKAILVKNAYIFTGEKAPKDMAHNVDQLIEFIEKYDGENL
                     IVFEIYQNKLDERKKLTKTLKKHARLKKIEQMSEEEIKKWIQSKLNENFKDIKRDALD
                     LFIELTGINFNIVSQEIEKLILFLGDRPTINKQDVNQIINRSLEQNVFLLTEYIQKRK
                     KEQAIHLVKDLITMKEEPIKLLALITSNYRLFYQCKILSQKGYSGQQIAKTIGVHPYR
                     VKLALGQVRHYQLDELLNIIDACAETDYKLKSSYMDKQLILELFILSL"
     gene            complement(13608..13859)
                     /locus_tag="NBH32_000904"
     CDS             complement(13608..13859)
                     /locus_tag="NBH32_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCD1514526.1"
                     /translation="MANIKSAIKRVKTTEKAEARNISQKSAMRTAVKNAKTAVSNNAD
                     NKNELVSLAVKLVDKAAQSNLIHSNKADRIKSQLMTANK"
     gene            14205..16028
                     /gene="lepA"
                     /locus_tag="NBH32_000905"
     CDS             14205..16028
                     /gene="lepA"
                     /locus_tag="NBH32_000905"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCD1514527.1"
                     /translation="MDNEQRLKRRENIRNFSIIAHIDHGKSTLADRILENTKSVETRD
                     MQDQLLDSMDLERERGITIKLNAVRLKYEAKDGNTYTFHLIDTPGHVDFTYEVSRSLA
                     ACEGAILVVDAAQGIEAQTLANVYLALDNELELLPVINKIDLPAAEPERVKQEIEDMI
                     GLDQDDVVLASAKSNIGIEEILEKIVEVVPAPDGDPEAPLKALIFDSEYDPYRGVISS
                     IRIVDGVVKAGDKIRMMATGKEFEVTEVGINTPKQLPVDELTVGDVGYIIASIKNVDD
                     SRVGDTITLASRPASEPLQGYKKMNPMVYCGLFPIDNKNYNDLREALEKLQLNDASLE
                     FEPESSQALGFGYRTGFLGMLHMEIIQERIEREFGIELIATAPSVIYQCILRDGSEVT
                     VDNPAQMPDRDKIDKIFEPYVRATMMVPNDYVGAVMELCQRKRGQFINMDYLDDIRVN
                     IVYELPLAEVVFDFFDQLKSNTKGYASFDYEFIENKESNLVKMDILLNGDKVDALSFI
                     VHRDFAYERGKALVEKLKTLIPRQQFEVPVQAAIGQKIVARTNIKSMGKNVLAKCYGG
                     DISRKRKLLEKQKAGKAKMKAVGNVEIPQDAFLAVLKMDDE"
     gene            16536..17660
                     /locus_tag="NBH32_000906"
     CDS             16536..17660
                     /locus_tag="NBH32_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCD1514528.1"
                     /translation="MTVQSAYIHIPFCVRICTYCDFNKYFIQNQPVDEYLDALITEMS
                     TAKYRNLMTMYVGGGTPTALSINQLERLLKAIRDTFTITGEYTFEANPDELTKEKVQL
                     LEKYGVNRISMGVQTFKPELLSVLGRTHNTEDIYTSVLNAKNAGIKSISLDLMYHLPK
                     QTIEDFEQSLDLALDMDIQHISSYGLILEPKTQFYNMYRKGLLKLPNEDLGADMYQLL
                     MSKIEQSPFHQYEISNFALDGHESEHNKVYWFNEEYYGFGAGASGYVDGVRYTNINPV
                     KHYIKAINKESKAILVSNKPSLTERMEEEMFLGLRLNEGVSSSRFQKKFDQSIESVFG
                     QTINNLKEKELILEKNDAIALTKRGKVIGNEVFEAFLIND"
     gene            17761..18738
                     /gene="hrcA"
                     /locus_tag="NBH32_000907"
     CDS             17761..18738
                     /gene="hrcA"
                     /locus_tag="NBH32_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcription repressor HrcA"
                     /protein_id="HCD1514529.1"
                     /translation="MITDRQLSILNAIVEDYVDFGQPVGSKTLIERHNLNVSPATIRN
                     EMKQLEDLNYIEKTHSSSGRSPSQLGFRYYVNRLLEQTSHQKTNKLRRLNQLLVENQY
                     DVSSALTYFADELSNISQYTTLVVHPNHKQDIINNVHLIRANPNLVIMVIVFSSGHVE
                     HVHLASDIPFNNDKLNTISNFVTNKLTEFNQNLQDDIVSFVQSEQEEIFINQLLNTMN
                     NHISNQSNSIYMGGKVKLIDALNESNVSSIQPILQYIESNRIAELLQDISSPNINVKI
                     GNEIDDSLSDISIVTSQYHFDETLKGQIAVIGPTAMHYQNVIQLLNRIW"
     gene            18770..19396
                     /gene="grpE"
                     /locus_tag="NBH32_000908"
     CDS             18770..19396
                     /gene="grpE"
                     /locus_tag="NBH32_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCD1514530.1"
                     /translation="MTNKDESVEKNTESTVEETNVKQNIDDSVEQAEESKGHLQDEAI
                     EETSDENVIEEIDPKDQKINELQQLADENEEKYLRLYAEFENYKRRIQKENEINKTYQ
                     AQRVLTDILPAIDNIERALQIEGDDETFKSLQKGVQMVHESLINALKDNGLEVIKTEG
                     EAFDPNIHQAVVQDDNPDFESGEITQELQKGYKLKDRVLRPSMVKVNQ"
     gene            19465..21297
                     /gene="dnaK"
                     /locus_tag="NBH32_000909"
     CDS             19465..21297
                     /gene="dnaK"
                     /locus_tag="NBH32_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCD1514531.1"
                     /translation="MSKIIGIDLGTTNSCVTVLEGDEPKVIQNPEGSRTTPSVVAFKN
                     GETQVGEVAKRQAITNPNTVQSIKRHMGTDYKVDIEGKSYTPQEISAMILQNLKNTAE
                     SYLGEKVDKAVITVPAYFNDAERQATKDAGKIAGLEVERIINEPTAAALAYGLDKTDK
                     DEKVLVFDLGGGTFDVSILELGDGVFEVLSTAGDNKLGGDDFDQVIIDYLVAEFKKEN
                     GVDLSQDKMALQRLKDAAEKAKKDLSGVSQTQISLPFISAGENGPLHLEVNLTRSKFE
                     ELSDSLIRRTMEPTRQAMKDAGLTNSDIDEVILVGGSTRIPAVQEAVKKEIGKEPNKG
                     VNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIEILGGRMNTLIERNTTIPTSK
                     SQIYSTAVDNQPSVDVHVLQGERPMAADNKTLGRFQLTDIPPAERGKPQIEVTFDIDK
                     NGIVNVTAKDLGTNKEQRITIQSSSSLSDEEIDRMVKDAEVNAEADKKRREEVDLRNE
                     ADSLVFQVEKTLTDLGENIGEEDKKSAEEKKDALKTALEGQDIEDIKSKKEELEKVIQ
                     ELSAKVYEQAAQQQQQAQGANGGQNNDSTVEDAEFKEVKDDDKK"
     gene            21433..22572
                     /gene="dnaJ"
                     /locus_tag="NBH32_000910"
     CDS             21433..22572
                     /gene="dnaJ"
                     /locus_tag="NBH32_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCD1514532.1"
                     /translation="MAKRDYYEVLGISKDASKDEIKKAYRKLSKKYHPDINKEEGADE
                     KFKEISEAYEVLSDDNKRASYDQFGHDGPQGFGGQGFNGSDFGGFSGFGGGGFEDIFS
                     SFFGGGRQRDPNAPQKGDDLQYTMTLTFEEAVFGTTKEISIRKDVTCETCHGDGAKPG
                     TSKKTCSYCNGAGHVAVEQNTILGRVRTEQVCPKCNGSGQEFEEACPTCHGKGTENKT
                     VKLEVKVPEGVDNEQQIRLAGEGSPGVNGGPAGDLYVVFRVKPSETFKRDGDDIYYKL
                     NVSFPQAALGDEIKIPTLNNEVMLTIPAGTQTGKQFRLKEKGIKNVHGYGYGDLYVDI
                     KVVTPTKLTDRQKELMKEFAQLNGEEINEQPSNFKDRAKRFFKGE"
     gene            22576..23514
                     /gene="prmA"
                     /locus_tag="NBH32_000911"
     CDS             22576..23514
                     /gene="prmA"
                     /locus_tag="NBH32_000911"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCD1514533.1"
                     /translation="MNWTELSIIINHEAVELATNILENHGSNGVVIEDSDDLINQPED
                     KYGEIYALKKEDYPDKGVRLKAYFNEMTYDDKLRQQIKDELLNLDELDQHNVQFSEQI
                     IAETDWENEWKNYFHPFRASKKFTIVPSWETYAKEADEELCIELDPGMAFGTGDHPTT
                     SMCLKAIETYVLPQHSVIDVGTGSGILSIASHLIGVKRIKALDIDEMAVSVAKENFRR
                     NHCETLIEAVPGNLLKDETEKFDIVIANILAHIIDEMIEDAYNTLNEGGYFITSGIIK
                     EKYEGIQSHMERVGFKIISEQHDNGWVCLVGQKVSE"
     gene            23516..24268
                     /locus_tag="NBH32_000912"
     CDS             23516..24268
                     /locus_tag="NBH32_000912"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCD1514534.1"
                     /translation="MQRYFIDQNADVSQRFFITKKEDIHHITNVMRYDVGSKIILTFN
                     DQTVYLCEIADITSDKIAVDLKEKQNINTELPVDVTICSGLIKADKYEWLLQKATELG
                     ASSFIAVSMERSIVKLNEAKVAKKIERWQKIIKEAAEQSYRLVIPSIQFESNLKLICD
                     TIDNYDYILIAYEEEAKDGELSNFKQTLQQFKAQDKVLMIFGPEGGLSENEISLFSNT
                     STVVGLGPRILRAETAPLYALSAISYENELMG"
     gene            24275..25621
                     /gene="mtaB"
                     /locus_tag="NBH32_000913"
     CDS             24275..25621
                     /gene="mtaB"
                     /locus_tag="NBH32_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HCD1514535.1"
                     /translation="MSTVAFHTLGCKVNHYETEAIWQLFKEANYERVDFEANADVFVI
                     NTCTVTNTGDKKSRQIIRRAIRQNPDAVICVTGCYAQTSSAEIMEIPGVDVVVGTQDR
                     HKLLGYIDEFRKERQPINGVGNIMKNRKYEELDVPYFTDRTRASLKIQEGCNNFCTFC
                     IIPWARGLMRSRDPEKVVEQATQLVNSGYKEIVLTGIHTGGYGQDLKDYNLAQLLRDL
                     ETINGLERIRISSIEASQLTDEVIDVLERSTKVVRHLHIPLQSGSDTVLKRMRRKYTM
                     DRFSERLTKLHKALPDLAVTSDVIVGFPGETEAEFQETYDFIVKHKFSELHVFPYSPR
                     IGTPAARMDDQIDEEIKNERVHKLITLSNQLGKLYASKFDQDVLEVIPEEQGDTEGTL
                     VGYADNYMKVQFEGDESLIGQIVKVKITQANYPLNEGQAIKVVDFATNKSDREVLV"
     gene            25914..26090
                     /locus_tag="NBH32_000914"
     CDS             25914..26090
                     /locus_tag="NBH32_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016998564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCD1514536.1"
                     /translation="MSKTVVRKNESLEDALRRFKRSVSKSGTIQEVRKREFYEKPSVK
                     RKKKSEAARKRKFK"
     gene            26334..27017
                     /locus_tag="NBH32_000915"
     CDS             26334..27017
                     /locus_tag="NBH32_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCD1514537.1"
                     /translation="MTTILESTAGDTWVEQVSNIIVQPIFTLILTCLTFLGFVYQLYS
                     KKINAAGIIATLSLLILFLGFLIQGNVNMHSILIFSIGVILVVIELFVVGAVIGIIGM
                     ILITISITTLGDNLLFMLANVIVALILTIVEWVILVKIFNRKIPFLDKVILKDSTNSE
                     SGYNSHDNRSHLVGKTAQTVTDLRPAGIIFCENERIDAVSDGNFILRNKTVKILEVEG
                     TRVVVREVD"
     gene            27035..28024
                     /gene="floA"
                     /locus_tag="NBH32_000916"
     CDS             27035..28024
                     /gene="floA"
                     /locus_tag="NBH32_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500186.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HCD1514538.1"
                     /translation="MFSLSFIVIAVIIVVALLILFSFVPIGLWISALAAGVHVGIGTL
                     VGMRLRRVSPRKVIAPLIKAHKAGLALTTNQLESHYLAGGNVDRVVDANIAAQRADID
                     LPFERAAAIDLAGRDVLEAVQMSVNPKVIETPFIAGVAMNGIEVKAKARITVRANIAR
                     LVGGAGEETIIARVGEGIVSTIGSSKHHTEVLENPDNISKTVLSKGLDSGTAFEILSI
                     DIADVDISKNIGADLQTEQALADKNIAQAKAEERRAMAVATEQEMKARVQEMHAKVVE
                     AESEVPLAMAEALRSGNISVKDYYNLKNIEADTGMRNAINKRTDQSDDESPEH"
     gene            28041..>28695
                     /locus_tag="NBH32_000917"
     CDS             28041..>28695
                     /locus_tag="NBH32_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron transporter"
                     /protein_id="HCD1514539.1"
                     /translation="MSVGILIFVISVIISIITTMRENSHKDRQNQKPPQKTSTDNEPK
                     KGGFFEEIERTFKEISEELNEEEKKSSKRKYDDTLPPLFDELPKEEPKSKPVVEPMAP
                     KKQPETKPMTEKPITVPKAEPVEQKHRPSRQDNSDEIRRQLEKSLRDDIKTIRTDIDR
                     EKEKQIAKMEKRARDIIEDKYLSERTKRLKLKQLLNSQNVEKDLTKSAFQFDKDEVIN
                     "
BASE COUNT        10659 a         3749 c         5342 g         8945 t
ORIGIN      
        1 gagtagagga ggctgcaaat gggttacaga actttaaaaa gtatttttca tgagcataac
       61 gaaagtaaaa tgaaagacga gtatactaaa agatttaatt ctttagcatc tttcaatact
      121 aatattaata tcatacccat ggaaaatgga aaaaaagtta atgatttgga atatcctcta
      181 ttctttatgg tgactaaaaa tctatcaaaa aaacaagaat taatatcaat taatagtaga
      241 aaaattgata gagcacttaa ttctttgcct tatgcagcta gagaacaata ttttaatgat
      301 ttattaatcg atgaattaca aagtactaat gaaattgaaa atgtatttag tactaaacaa
      361 gagattgcac atgcgttaaa taaccaagca tcagaatttc ttaagttcag aggcctcgtg
      421 gatcaatata aagagataga acttaataaa aaaattaaag ttgataatgt aagagacatt
      481 agagcgattt atgataaatt agtttcaaat gaaattaacg aacaagataa gttagatgga
      541 gagctatttc gtaaaaattt tgtcggtgta catgatgggt caacgaataa atatatacat
      601 gttgggttac aacctgaaac caaaattgtt gaatatatag gtgaaatgct aacattttta
      661 aaatattttg atgcgcctca gccgttcaaa atcatggcta gtcattatct gtttgaatat
      721 atacatcctt tttatgatgg caatggtaga gttggaagat ttataattgc caagctcttg
      781 agtgattatt atgataatta tactgctttg acattttcat atgttataaa taggaataaa
      841 tcgaaatatt acaaggcgtt tatgactgct tcaaatcatt tgaattgcgg tgatttgacg
      901 gaatttattg acacaatgct tgagttattg atagcaggac aagaaagaat acttgatgaa
      961 ttaataccaa aaatggatgc gactgaaaaa ttaacgcttt atttaactag tcattataaa
     1021 cagatagatt atgaattttt gtatctcctt tcaatggata aattgtttgg aaataaaaga
     1081 aatagattaa cattaattga tttagaaaac atattgggag taggaagagt aaaaattaat
     1141 aatacaatta aaaaatttgg taactactta gttaaaatta aaagtagacc aacaatttat
     1201 gagataagtg atgaatttct aaattcgatt ataaaataaa tgtaatatgt gattcagcta
     1261 tacttattgt atttgtaaga ttgcgtaatt gcttcattaa aaaaatatat ttgttgagag
     1321 tggtgcatga caaatgtact gctctttttg ttgtgtatca gtattgtgat gttttaatgc
     1381 gaatattata tgaatcatta tgaaatttaa taaaaataat tgaaataatt atcatttttt
     1441 ctgatatact gttaaacggt ttggaatttt taagtataca ctgtattggt tgatataact
     1501 caactaataa ttgcgaacag ggtatttcaa attggaaagt attatgagcg tgatacataa
     1561 tcaaaattgt aggctaaaga acctgagaca taaatcaatg ttcagtgctc tacaaagtta
     1621 tattggcagt agttgactga acgaaaatac gcttgtaaca agcttttttc aattctagtc
     1681 aaccttgccg gcggggcccc aacatagaag ctggcgaaaa gacagcttac aataatgtgc
     1741 aagttggggt gggacgacga aataaatttt tcgaaaatat catttctgtc ccactccctt
     1801 atcatttatg tctcgctctc aaatgtaaat tagtaattgt tttgggggag tttgaagtta
     1861 aatatttaac aggatttatt ttaatattat tgttagaagg aatttttaca aattcagcaa
     1921 gtgcaatcga atattcagac ttacatcata aaagtgaact tgattccaag cgtctatata
     1981 atgctaaggt gtcatttgct aatccaactc agcttgaaaa taaaaaaaca aacgatagac
     2041 tgttgaagca tgatttgtta tttcatgaca tgttcgtaaa tgatgcttgg aaaaaagatt
     2101 ttaaagttga attcgaaaat gaggcacttt caaagaaatt tgtaaataag gatatcgata
     2161 tatttgctgg aaattatgga tatggatgtc atgggggaga aaccaataaa acgctatgta
     2221 gttatggtgg tgttacttta agtgacaata ataaatacga tgattataag aatatacctt
     2281 gtaatttatg gattgacgga catcaaacag gaatagaact aactgcagta aaaacgaaaa
     2341 agaaaattgt taccattcaa gaattagatg ttcaattaag aaattatttg aatgagaagt
     2401 ataagttgta cgaactaggt ggagacattg ttaaagggta tgttaaatat cataatgatg
     2461 atgaaaaaaa tgtagaatat gatttttata atttaaatgg tgagtatggt catgaggtat
     2521 taaaaatgta tgctgataat aaaactatca atagtgacaa attgcattta gatatctatt
     2581 tattcaaatc ataatttaag taagttaatc attaatgatc tttgacagtg aaatataaag
     2641 tacctgtcat tttattgtta caaataaaaa cagcagtaag gaggctatca ctcagaaaag
     2701 tcttactgct gttttctatt agaattacct ttaatgcaca caggaattcg atacgaaaca
     2761 aatatgatta actgtgtaat tacaaaataa aactactata gcaaaaagaa gataaagacg
     2821 atgataataa atgcatcgaa tataattttc ttgatgtttt tatcaacaac atcaggtaag
     2881 gacataagta taaatcctag aattagagtg tagcgacata tgttcataat tgttgctgta
     2941 aactcgatat taaatgaaaa ctttaaaaat atacttataa ataaaatgac aaacgacgca
     3001 attgcaataa aactgccgta tgtagcaatc tttgttacga gactattttg attattttca
     3061 gccattgtag aatcctctta tttcatcatt attattttaa acatatcatt ttcctgttat
     3121 ttaataaagt ttcttgacac aattagctga tgtaattgat ttgattagtt tttgaaaaat
     3181 agacaattta gaagcatcga tttccttgtt atgagttcat aaataattat ttatgatacc
     3241 acgttattgt gaatgttttt ttaaaaaata aagcaaaagt tttatacagt aacgaaaatc
     3301 atgatacttg tcgttgtagt gattaaaatg tgataaaata acaaatgcta ggatttttta
     3361 aaagaggtga atcaaaggat gattggtata attggtgcca tggaagaaga aataacaata
     3421 ttaaaaaata aattaacaca attaagcgaa atttcagttg cacatgttaa attttatact
     3481 ggcattttaa aagatagaga agtagtgatt acccaaagcg gcattggaaa agttaatgct
     3541 gcaatttcta cgacattatt aattaataag tttaaaccgg acatcattat taatacaggt
     3601 tctgctggag ctttagatga aagtttaaat gtaggtgacg ttcttataag tgatgatgta
     3661 aaatatcatg atgcagatgc aacagcattt ggttatgaat atggacaagt accacagatg
     3721 ccggtagcat ttcaatcaag taaaccttta atagataaag tatctcaagt tgtacaacaa
     3781 caacaattaa cagctaaagt aggcttaatt gtaagtggtg atagctttat cggtagtgtt
     3841 gaacaacgcc aaaaaattaa aaaagcattt ccaaatgcga tggcggttga aatggaagca
     3901 actgcaattg cacaaacatg ttatcaattt aatgtaccat ttgttgtagt tcgtgcagtt
     3961 tcagacttag caaatggaga agcggaaatg agcttcgaag catttttaga aaaagcagct
     4021 gtatcatcaa gtcaaactgt tgaagcatta gtgtctcaat tataaaaagg taaaggtgag
     4081 ttaaatgggt ttagttcgca agttttttat gccgaattca tatgttcaat caatatttca
     4141 aattgattta gacaagttag tggacaaagg cgttaaaggt attattacag atttagataa
     4201 tacgctagta ggttgggatg ttaaagaacc tacagaacgt gttaaagcat ggtttaagga
     4261 agctaatgaa aaaggaatca ctattacaat cgtgtctaat aataatgagt ctcgtgttgc
     4321 tagttttagt cagcatttag acatcgattt tatttttaaa gcgagaaagc caatggggaa
     4381 agcgtttgat aaagcaataa ctaagatgaa tatcagacca gatcaaactg ttgttatagg
     4441 tgaccaaatg cttactgatg tatttggtgg taatcgtcga ggtttatata caattatggt
     4501 cgttccagtt aaacgaactg atggctttat tactaagttt aataggttaa ttgaaagacg
     4561 attattacgt catttcagta aaaaaggtta tatcacatgg gaggaaaatt gattgtctga
     4621 cattttaaaa tgtatcggtt gtggtgcgcc acttcaatct gaagataaaa ataaacctgg
     4681 ttttgtacca gagcataata tgtttcgtga tgacgtgatt tgcagacgtt gtttccgctt
     4741 gaaaaattat aatgaagttc aagatgtagg attagaaagt gaagactttt taaaattatt
     4801 atcaggactt gcggataaaa agggtattgt cgtcaatgtc gtggatgtat ttgactttga
     4861 aggatcattt attaatgcag ttaaacgtat tgtcggaaac aaaaaaatca ttttagcagc
     4921 aaataaattg gatttattgc cgaaacaaat taataaacgt cgagttaaag aatggttaaa
     4981 acgaacagca agaaaatatg gtttggaagc tgacgatgtc gtattaattt cagctgaaaa
     5041 aggctggggc atagacgact tattatcatc aattgcgaat attcgagaaa atgaagatgt
     5101 gtatattgta gggacaacga atgttgggaa atctacattg attaataaac tgattgaagc
     5161 tagtgttggt gaaaaagatg tagtaacaac ttcaagattt cctggaacaa ctttagatat
     5221 gatagatatt cctttagatg aaacatcatt tatgtatgat acaccaggta ttattcaaga
     5281 tcatcaaatg acgcatttag ttagtgaaaa agaattgaaa attattatgc ctaagaaaga
     5341 aataaaacaa cgcgtatatc aattaaatga ggcgcaaaca ttattcttcg gcggtctagc
     5401 gcgcatagat tatgtatcag gtggtaaacg tccgttagtt tgtttctttt ctaatgactt
     5461 gaatatacat cgtactaaaa cggagaaggc taatgattta tggcgtaatc aacttggcga
     5521 tttattaacg ccacctggaa atccacaaaa ttttgatctt aatgaggtaa aggctgttag
     5581 acttgaaaca ggcaaagaga aacgcgatgt tatgatctct ggtctaggct ttataactat
     5641 aggaccagga gctaaagtaa tcgttcgtgt tcctaaaaat gttgatgttg tattaagaaa
     5701 ttctatttta taaggtgatt taaaaaatga aatttgcagt tatcggaaat cctatttcac
     5761 attccttgtc gcccgttatg catagagcaa attttaattc tttaggatta gatgatactt
     5821 atgaagcttt aaatattcca attgaagatt ttcatttaat taaagaaatt atttcgaaaa
     5881 aagaattaga tggctttaat atcacaattc ctcataaaga gcgtatcata ccgtatttag
     5941 atcatgttga tgaacaagcg attaatgcag gtgcagttaa cactgttttg ataaaagatg
     6001 gcaagtggat agggtataat acagatggta ttggttatgt taaaggattg cacagcgttt
     6061 atccagattt agaaaatgca tacattttaa ttttgggcgc aggtggtgca agtaaaggta
     6121 ttgcttatga attagcaaaa tttgtaaagc ccaaattaac tgttgcgaat agaacgatgg
     6181 ctcgttttga atcttggaat ttaaatataa accaaatttc aatagcagat gctgaaaagt
     6241 atttagctga attcgatatc gttattaata caacaccagc gggtatggct ggaaataacg
     6301 aaagtattat taatttaaag catctttctc ccaatacttt aatgagtgat attgtttata
     6361 taccgtataa aacacctatt ttagaggaag cagagcgcaa gggaaaccat atttataatg
     6421 gcttagatat gtttgtttac cagggtgcgg aaagctttaa aatttggact aataaagatg
     6481 ctgatattaa ttctatgaaa acagcagttt tacaacaatt aaaaggagaa taacatatgc
     6541 ttactggcaa acaaaaaaga tacttaagaa gtttagcaca caatattgat ccgatttttc
     6601 aaattggaaa aggcggtatc aacgaaaata tgattaaaca aatagatgat acgttagaaa
     6661 acagagaatt gattaaagtg catgtactac aaaataactt tgatgataaa aaagaattag
     6721 ctgaaacatt aagcgaagct acgcgtagtg aattagtgca agtgattgga tctatgatag
     6781 tgatttatag agaatctaaa gaaaataaag aaattgaatt gccataataa tgaaaaagat
     6841 agtactttac ggcggtcagt ttaaccctat ccatactgca catatgatag tagctagcga
     6901 agtatttcat gaattacagc cagatgaatt ttatttttta cctagtttta tgtctccatt
     6961 gaaaaagcac aataatttta tagatgttca gcacagatta acaatgatac agatgattat
     7021 cgacgagctt ggttttggag atatttgcga cgatgaaatt aaacgtggtg gtcagagtta
     7081 tacctatgac acgatcaagg cattcaagga gcaacacaaa gacagtgagt tgtactttgt
     7141 tattgggacg gatcagtata accaactaga gaaatggtat caaattgaat acttaaaaga
     7201 aatggttact tttgtagttg taaatcgaga caaaaatagt caaaatgttg aaaatgctat
     7261 gattgcaatt cagataccta gggtagatat aagttcgaca atgattcgac aaagagttag
     7321 tgaagggaaa tctatccaag ttcttgttcc taaatccgtt gaaaactata ttaaggggga
     7381 aggattatat gaacattgaa aaagcaaaac ggcttgcaaa agagaaactg ccagagaaac
     7441 gttataatca ttctttaaga gttgcagaaa cagctattaa attggcagaa atttatgatg
     7501 gagacactag taaagtagaa ttagcaggtg tattacatga tttctgtaag tatgatgatt
     7561 taggtaaaat gtatcaaatt gttcgacaat acgaattagg taatgaccta ctgagttatg
     7621 gtagtgaaat attgcatggc cctgtgtgtg cagcaatcat ggaacatgaa tatggtatca
     7681 atgatgagga agtattaatg gctatcaaat accatactac tggacgtcaa caaatgacaa
     7741 aaactgaaaa actgattttt attgcagatt acatcgaacc tggaagaaca atcccaggag
     7801 ttgatgatat tcgagatatg gcatacaatc aaggtagttt agataagaca atttatgaaa
     7861 tttctaaacg cacagtacta tttttaatac agaaagatat tacggtatat aataagacga
     7921 ttgactgttt aaattattat aactatagtg acgaaagaat aaaggatgat taaatgaatt
     7981 cacaagaatt attagcaatt gctgtggatg caattgacaa taaaaaaggc gaagatacga
     8041 tttctttaga aatgaaaggt atcagcgata tgacagatta ttttgttgta acgcacggaa
     8101 ataatgaacg acaagttcaa gcgattgcta gagcggtgaa agaagtagcc aatgaacaaa
     8161 atatagaagt aaaacgtatg gaaggataca atgaagcgcg ttggatatta attgacttag
     8221 ctgatgttgt ggtacatgtt ttccataaag acgaaagaaa ttattataat attgaaaagt
     8281 tatatcaaga tgcaccatta gaatcatata gtcaggttgc gtattaatta tgtcgcaata
     8341 tgcagaaatg agcctagtgt acgatcaatt gactcaagat caaccatatg aaaaatggtt
     8401 tgaaattgta aaaaatcact gcaaagatga atcaaatatt ttagatattg gatgcggtac
     8461 tggtagttta acagttcaat tagaagcttt aggtaatgtt actggtatgg atttaagtgt
     8521 tgatatgttg actgttgcag ctcaaaaatc agcaaatgta aattggcttg aaggagatat
     8581 gacatctttt gatttgcaac aacaatttga tatcatcact attttttgtg actctctaaa
     8641 ttatttgcaa gatgaaactg cagtgattga aacattcatc aatgtttatc atcatctgac
     8701 tgattcaggt gtatttattt ttgatgtaca tactgtttat aaaatgatga atttgtttaa
     8761 taatcaaagt tatattgatg ataaagggga cattttttta gcatgggatg ctgtgcaagg
     8821 agatttacct ttaagtgttt atcatgatat gacatttttc attcgacatg aagatgaaac
     8881 atattcaagg tttgatgagt ctcattttca gagaacattt gatgaaaaga catatttgtc
     8941 ttggttagct caagttggtt ttaaacaagt tgaaacattt actgatttta atatagatga
     9001 acataatgaa gatgcagaaa gattgttttt cattgcgaaa aaataaaagt aaaaccactc
     9061 gctcagacgt gtataaagtt gaggagtggt tttattgtat caatttttat tacgctataa
     9121 agatttttta actcagtgga agttatatat tataagtgct gttgttttaa ttatggtatt
     9181 aattggtttt atattctgga gacaagatga ttatacttca agaaattttg aaaataaaga
     9241 tactgctctg aaacaaagca ctagtgaaaa tagtagtttg tccaaattag aagatgtcca
     9301 ggtcaaagat ggagataatt ccaaaaataa aggtcctgta tatgtcgatg taaaaggtgc
     9361 tgttaaacat cctaatgttt ataaaatgac atctaaggat agagtagttg atttacttga
     9421 taaagcacaa ttattggagg atgcagatgt aagtcaaatt aatttgtctg aaaaattaac
     9481 agatcaaaaa atgattttca tacctcataa aggacaaaag aatgttgaac cacaaattgg
     9541 agtaaacagt gtgcatgtaa aaaatgggaa cacaaataat actaaagtaa atttaaatac
     9601 cgcatctgta tcagaattga tgtctgttcc tggagtaggg caagctaaag ctaatgcaat
     9661 tgttgaatat cgcaaccaac aaggtgcatt tcaagaaatt gacgatttga aaaaagtaaa
     9721 aggttttgga agtaaaactt ttgataaact gaaatcttat ttcacgatat aatggattat
     9781 tttaattgca aattttattt tttgtgatta atcttatatt attagaaaat gttactaaat
     9841 tttaagtatg gaaggtgaac atcttggaaa gaatcaaatg ggaagaatat tttatggcac
     9901 aaagtcattt gctagcatta cgttcaactt gtcaaagatt atctgtaggt gcaacgattg
     9961 ttaaggataa tcgtattatt gctggtggtt ataatggctc tgtagctggc gaggtgcatt
    10021 gtatagatga aggatgttta attgaagatg gacattgtat cagaacgata catgcagaaa
    10081 tgaatgcttt attacaatgt gcaaaacaag gtgtatctac tgaaggtgca acaatctatg
    10141 ttactcattt tccatgccta aattgtacaa agtcaattat tcaagcaggt ataaagcgta
    10201 tctactatgc agaagattat cataaccatg aatatgcaac taaattactc aaacaatctg
    10261 gtattgaatt taaaaaaatt ccattttcac cagaatatgt tgctaaatat ctgactaaag
    10321 gttaatgttt tgctgtatgt cgcgttatca atgattgtag gagtgctttg gaattctagc
    10381 aaagtgctct ctacatttct tttcatttta cttttgtata ttacttatcg taaaaataaa
    10441 atcgtttatg cccctatttc tctcttttta atcattttct cctcatggta tttacattat
    10501 tcacaacaag caatatttaa ttatatcaat tatattgaac gtaattctca gtttaatgag
    10561 cgtgctcaag taatccaaat tcaacgtcaa ggtagtgaca catataaagg taggttgagt
    10621 ttaaaaaatg aaatatatcc tttcttttta acaaataaaa agaattttga tttaaagaaa
    10681 attgaaagtc gtaattgtat tgttaaagga caattcaaag ttaatgacaa taagtttgta
    10741 actcttaaat tacaaagtat agttgtacaa agctgcctag aatcgaaccg gtctaattta
    10801 attgagaaac ataaacagtt tataatgaat cgaatttatg attcgggtat taagtttccg
    10861 gatcgtatta tggcattgat tactggtgac gtaaaagaaa ttaatgagca atttaaggaa
    10921 cgtgttaaag agataggtat atatcatttg ctggcagtta gtggctcgca tatagctgca
    10981 attgtattct taatttacca acctttaaaa cgattaaatt tacctttatt tgtcattaaa
    11041 ggaattacaa tcattgtatt agctttattt gctcaataca caaattatgc acctagtgct
    11101 gtaagagcta taataatgac aactcttgta ctgcttatta ctaagcaaat taaaataaag
    11161 ggtattcagc tattagcatt tgcatttata attatgttta ttttaaatcc actagttgtt
    11221 tatgatattg gatttcaatt ttcattcatc atttcatttt ttattatgct actttttcct
    11281 tttttacagc aattgtcaaa gttacaatca ttattcataa ttacgtttat tgcacaatta
    11341 gcttcattta tcgttgccat tccaaacttt catcaacttc aatgggtggg atttttatct
    11401 aatttgattt ttgtaccgta ctattcgatt atattgtttc cgctatctat tttattcttt
    11461 attacaagtc attttattgt gggattaacg ccgctaaatt acttggttga cctaagtttt
    11521 aattttcatg actggttact agacctattc acaagaatca agcaatcaca tttttctgtt
    11581 cccaagttta atgattggat atttataata tttataattt ctgtttatta catattttgg
    11641 ttattggcta aacgtaaata tatattggtt acggtttgga ctataattat tctgacatta
    11701 ttaataacgt ttccaacaaa ttcacatcac aaaattacaa tgttaaatgt ggggcaggga
    11761 gacagtattt tatatgaagg tggtaagaac caaaatgtct tgattgatac aggtgggaaa
    11821 gtgattgatg atactaaaca acctagttat tcaatttcta aatatcatat tttaccaacg
    11881 ctaaatgaaa gagggataaa tgaattagag tatctaattt taacacatcc acacaatgac
    11941 catattggtg aagtggaata tattattagt catattaaaa ttaaacatat agtgatatac
    12001 aataagggat atagtagtaa tacattgatg ttattatcga aattaagcca taagtataac
    12061 attaaactta tggatgtaag acaagttagt agttttaaac ttggagatag tagttttcta
    12121 tttttttgat agttttattc caaatagccg agataaaaat gaatattcta ttattactat
    12181 gattacatat caaaataaaa aagttttatt aatgggcgat gctagtaaaa ataatgaatc
    12241 tttactacta aaaaaatata acttgccgga gattgatatt ttaaaagtag gacatcatgg
    12301 gagcaagaca agtagttcta aagaatttat agagatgatt aagcctaaaa taagtttgat
    12361 ttcttctggg aagaacaata tgtatcatct tcctaatata gaagttgtta aacgattgca
    12421 aaggattcgc agtcgcattt acaatagtca acaaaacggt caagttacaa ttgacttaga
    12481 tgataattta aaagttgatt caaactctta tggaaatgca agtggtttat agatacagaa
    12541 acatgatata atctattgat gtgtcaaacg aatgaaaggg tgtaatgaat gagcgacaat
    12601 attgtagcta tttatggaga tgtgcctgaa ttggttgaaa aacaaagtgc agaaatcata
    12661 tcacaatttt tgaaaagtga tagagatgac tttaactttg tgaaatataa tttatacgaa
    12721 acagagattg caccaattgt tgaagaaaca ttaacattgc ctttcttttc agataaaaaa
    12781 gcaattttgg ttaaaaatgc atatatattt acaggtgaaa aagcgccaaa agatatggct
    12841 cataatgtag atcaattaat agaatttatt gaaaaatatg atggcgaaaa tttgattgtc
    12901 tttgagatat atcaaaataa acttgatgaa agaaaaaagt taactaaaac tctaaaaaag
    12961 catgcaaggc ttaaaaaaat agagcaaatg tctgaagaag aaataaaaaa atggattcaa
    13021 agtaaattaa atgagaattt caaagatatc aaaagagatg cattagattt atttattgag
    13081 ttgacaggta ttaactttaa tattgtctca caagagatag aaaagttgat tttattttta
    13141 ggcgatagac caacaattaa taagcaggat gttaaccaaa ttattaatag aagtttagaa
    13201 caaaatgtat ttttactgac tgaatacatt cagaaaagaa agaaagaaca agcaattcat
    13261 ttagtaaaag atttaataac tatgaaagaa gaacctatta aattacttgc actaattaca
    13321 agtaattacc gattatttta tcaatgtaag attttgagtc aaaaaggata tagtggacag
    13381 caaattgcta aaacaatagg cgtacatcca tacagagtaa aattagcgtt aggacaagta
    13441 agacattatc aacttgatga attattgaat attatagatg cttgtgcgga aactgattat
    13501 aaacttaaat catcatatat ggataaacag ttaatactgg aattattcat tctatcttta
    13561 taaaaaataa aagttcaagc atgcgcttga acttttattt aaaaagatta tttatttgca
    13621 gtcattaatt gtgatttaat acggtcagct ttgtttgaat gtattaaatt actttgagca
    13681 gctttgtcta ctaacttaac tgctaagctt actaattcat ttttattatc agcgttattt
    13741 gaaacagctg ttttagcgtt tttaactgct gtacgcattg cactcttttg tgaaatgttg
    13801 cgtgcttcag ctttttcagt tgtttttacg cgtttaattg cagatttgat atttgccatt
    13861 tctgtcacct cctaaaagtg agttcatctt atcaaaattt tatttgatta caacaagaaa
    13921 tattttaaca aaagtctaac cgttgtgcaa tcatttattt gatggggtta gcttaaaatt
    13981 ttgcagattt aataaattgt gttggaaaca caatcaatac gttttataat tataagtacg
    14041 taattaacat attaacatat cgcaagtatg tatttaaata aagattgtta taatttcaaa
    14101 gttcatccaa gattatggcg tttgcattta cctattaaaa acgttattat atcaaagatg
    14161 cgaaagataa tacgggttta ttttatgaaa gtgagaagga taaaatggat aatgagcaac
    14221 gcttaaaaag aagagagaat ataaggaatt tctcgattat agcacatatt gaccacggaa
    14281 aatctacatt ggctgataga attttagaaa ataccaaatc agttgaaaca agagatatgc
    14341 aagatcagtt actagattca atggatttag aaagagaacg tggtattaca atcaaattaa
    14401 acgcagtacg tttaaagtac gaagctaaag atggaaatac ttatacattc catttaatcg
    14461 atacgcctgg acacgtcgat tttacatatg aagtgtcacg ttctttggca gcttgtgagg
    14521 gtgcgatttt agtagtagat gcggctcaag gtatcgaagc acaaacatta gcaaatgttt
    14581 atttggcatt agataatgag ttagagttat tgcctgttat taacaaaatt gatttacctg
    14641 ctgcagaacc tgaacgcgtg aaacaagaaa ttgaggatat gataggttta gaccaagacg
    14701 atgttgtttt agcaagtgct aaatctaaca ttggaattga agagatacta gagaaaatag
    14761 ttgaagttgt gccagctcca gatggtgacc cagaagcacc actaaaagcg ttaatatttg
    14821 attctgagta tgatccatat agaggggtaa tttcatcgat aagaattgta gacggtgttg
    14881 ttaaagccgg ggataaaatt cgaatgatgg ccactggtaa agagttcgaa gtaacagaag
    14941 ttggaattaa tacacctaag cagcttccag ttgatgaatt aacagttggt gatgttggtt
    15001 atattattgc aagtattaaa aatgttgatg attctagggt tggtgacaca atcacattag
    15061 ctagtagacc tgcatcagaa ccattgcaag gttataagaa aatgaatcca atggtatatt
    15121 gcggactgtt cccaatagat aacaaaaatt ataatgattt aagagaagca ttagaaaaat
    15181 tacaattgaa tgatgcatca ttagaatttg agcctgaatc atcacaagca ttaggttttg
    15241 gttatagaac tggtttctta ggtatgttac acatggaaat aattcaagaa agaattgaaa
    15301 gagaatttgg tattgaatta attgcaaccg caccatctgt aatttatcaa tgtattttaa
    15361 gggacggttc agaagtgacg gttgataacc cagcacaaat gccagatcgt gataaaattg
    15421 ataaaatatt tgagccatat gttcgagcaa ctatgatggt tccaaatgac tatgtcggtg
    15481 cagtaatgga attatgtcaa cgtaaacgtg gacaatttat aaatatggac tatttagatg
    15541 atattcgtgt aaatattgtt tatgaattac ctttagctga agttgtattt gatttcttcg
    15601 atcaacttaa atctaatact aaaggatatg catcatttga ttatgaattc atcgaaaata
    15661 aagaaagtaa tttagtcaag atggatattt tattaaatgg tgataaagtg gatgcgctaa
    15721 gcttcatagt tcatagagat tttgcgtatg aacgtggtaa agcattagtt gaaaaactta
    15781 aaacgttaat tccaagacag caatttgaag tacctgtaca ggctgcaata ggacaaaaaa
    15841 ttgtagcgcg tacaaatatt aaatcaatgg gtaaaaacgt tttagctaaa tgttatggcg
    15901 gtgacataag ccgtaaacgt aaattacttg aaaaacaaaa agcaggtaaa gctaagatga
    15961 aagcagttgg taatgttgaa attccacagg atgctttctt ggctgtgttg aaaatggatg
    16021 acgaataagt ttttaaaaaa tctattaaca atttacaatg aataaagttt aataactaaa
    16081 aagagggagc ctaggataaa ttaacgtcct gggctttaca atgttatatt ggcagccatc
    16141 gacagagtta aaatgagctt ataacaatgg ggccccaaca tagaagctga cgaaaagtca
    16201 gcttacaata atgtgcaagt tggggtggga cgacgaaata aattttgtga aaatatcatt
    16261 tctgtcccac tcccttatgc atgagtttaa ctcatgtaat tctattttta agtacatatt
    16321 acctctggct aatgtgtaag agccactaca taataaatca ttagtggttc tttattattt
    16381 ctatctcact ccctcttaac atttataaat attaaaatga atcgatatat tagacaatca
    16441 ttgattaaac gttaaagtta aaagtaagaa taatagcaga tagtccaaca ggatgtagcc
    16501 gattggataa acagtctgag aagcggggca ttaaaatgac ggtacaaagt gcatatatac
    16561 atattccatt ttgtgtaaga atatgtacat attgtgattt caataaatat tttatacaga
    16621 atcaacctgt agatgagtac ttagatgcac taataacaga aatgtctaca gccaaatata
    16681 ggaacttaat gaccatgtat gtaggtggtg gcacaccaac ggccctttct attaatcagt
    16741 tggaaagatt acttaaagca atacgtgata cgtttacaat cacaggcgag tatacatttg
    16801 aagccaatcc tgatgagtta actaaagaga aagtccaact attagagaaa tatggagtaa
    16861 acagaatttc aatgggcgtt caaacattca agccggagtt attgtctgtt ttaggtagaa
    16921 cgcacaatac tgaagatatt tacacttcgg tgttaaatgc taaaaacgca ggtattaaat
    16981 caatcagttt agatttaatg tatcatttac cgaaacagac gattgaagat tttgaacaaa
    17041 gtttagatct agctttagat atggatattc aacatatttc gagttacggc ttaatacttg
    17101 aacctaaaac ccaattttat aatatgtata gaaaagggtt gctcaaactt cctaatgagg
    17161 atttaggtgc tgacatgtat cagttgctga tgtctaagat agaacaatct cctttccatc
    17221 aatacgaaat atctaatttt gcattagatg gccatgaatc agaacataat aaggtttact
    17281 ggtttaatga ggaatattat ggatttggag caggtgcaag tggttatgta gatggtgtgc
    17341 gttatacgaa tatcaatcca gtgaaacatt atatcaaagc tataaataaa gaaagtaaag
    17401 caattttagt atcaaataaa ccttctttga ctgagagaat ggaagaagaa atgtttcttg
    17461 ggttgcgttt aaatgaaggt gtgagtagta gtaggttcca aaagaagttt gaccaatcta
    17521 ttgaaagtgt ctttggtcaa acaataaata atttaaaaga gaaggaatta attttagaaa
    17581 agaacgatgc gattgcacta acaaagcgag ggaaagtcat cggtaatgag gtttttgaag
    17641 ctttcctaat aaatgattaa aaaaaattga aatttcgagt ctttaacatt gacttacttt
    17701 gaccaatttg ataaattata attagcactt gagataagtg agtgctaatg aggtgaaaac
    17761 atgattacag ataggcaatt gagtatatta aacgcaattg ttgaggatta tgttgatttt
    17821 ggacaacccg ttggttctaa aacactaatt gagcgacata acttgaatgt tagtcctgct
    17881 acaattagaa atgagatgaa acagcttgaa gatttaaact atatcgaaaa gacacatagt
    17941 tcttcagggc gttcgccatc acaattaggt tttaggtatt atgtcaatcg tttacttgaa
    18001 caaacatctc atcaaaaaac aaataaatta agacgattaa atcaattgtt agttgagaat
    18061 caatatgatg tatcatcagc attgacatat tttgcagatg aattatcaaa tatatctcaa
    18121 tatacaactt tagttgttca tcctaatcat aaacaagata ttatcaataa tgtacacttg
    18181 attcgtgcta atcctaattt agttataatg gttatcgtat tttcatcagg tcatgtagag
    18241 catgtacatc ttgcttcaga cataccgttt aataacgaca aactaaatac aatatcaaat
    18301 tttgttacaa acaaactaac tgagtttaat cagaatttac aagatgatat tgtctctttt
    18361 gttcaatcag agcaggaaga aatatttatc aatcaattgt taaatacgat gaataatcat
    18421 atttctaatc aaagtaatag catttatatg ggtggaaagg ttaaacttat tgatgcattg
    18481 aatgaaagta atgtatcttc aattcagccc attctacaat atatagaatc aaatagaatt
    18541 gcagaattat tacaagatat atcttcacca aatattaatg taaaaattgg taacgaaatt
    18601 gatgacagct taagtgatat ttcaattgta acaagtcaat atcattttga tgaaacgtta
    18661 aaaggtcaaa tcgctgtgat tggacctaca gctatgcatt atcaaaatgt cattcaatta
    18721 ttaaatagaa tttggtaatg atggattaga aagtattgga ggacagaaaa tgacaaataa
    18781 agacgaatca gttgaaaaaa acactgaatc aacagttgaa gaaacaaacg tcaaacaaaa
    18841 tattgatgat tcagttgaac aagctgaaga aagtaaaggt catttacaag atgaagcaat
    18901 agaagaaacg tctgacgaga atgttattga agaaatagat ccaaaagatc aaaaaattaa
    18961 tgaacttcaa caattagcag atgaaaacga agagaaatat ttaaggctct acgctgagtt
    19021 tgaaaattat aagcgtagaa ttcaaaaaga aaatgaaata aacaaaacat atcaagcaca
    19081 acgtgtgtta acagatattt taccagcaat agacaatata gaacgtgcac ttcaaattga
    19141 aggtgatgat gagactttta aatctcttca aaaaggtgta caaatggtgc atgaaagttt
    19201 gattaacgca ctaaaagata atggtcttga agttattaaa actgaaggtg aagcatttga
    19261 tccaaatatt caccaagctg tagttcaaga tgataaccct gattttgaat ctggcgaaat
    19321 cactcaagaa ctacaaaaag gatacaagct taaagataga gtattaagac catcaatggt
    19381 caaagtaaac caataactta aatttggcga aaagacattg tttaaaatta aattaattta
    19441 atgattaaat ggaggaattt tattatgagt aaaattattg gtatagactt aggtacaaca
    19501 aattcatgtg taacagtatt agaaggcgat gagccaaaag taattcaaaa ccctgaaggt
    19561 tcacgtacaa caccatctgt tgtagctttc aaaaatggag aaactcaagt tggtgaagta
    19621 gctaaacgtc aagctattac aaatccaaac actgttcaat ctattaaacg tcatatgggt
    19681 actgattata aagtagatat tgaaggtaaa tcatacacac cacaagaaat ctcagctatg
    19741 attttacaaa acttaaaaaa tacagctgaa agctatttag gtgagaaagt tgacaaagct
    19801 gtaattacag tacctgcata ctttaacgat gctgaacgtc aagcaactaa agatgctggt
    19861 aaaattgctg gtttagaagt tgagcgtatc attaatgaac caacagctgc agcattagca
    19921 tatggtttag acaaaactga taaagatgaa aaagttcttg tttttgactt aggtggcggt
    19981 acatttgacg tatctatcct agaattaggt gacggtgtat tcgaagtact atcaacagcc
    20041 ggtgacaaca aacttggcgg tgatgatttt gaccaagtaa ttattgacta cctagttgca
    20101 gaattcaaaa aagaaaatgg cgtagactta tctcaagata aaatggcatt acaacgtttg
    20161 aaagatgctg ctgaaaaagc taaaaaagac ttatcaggtg tatcacaaac tcaaatctca
    20221 ttaccattta tctcagctgg tgaaaacggt ccattacact tagaagtaaa cttaactcgt
    20281 tctaaatttg aggaattatc agattcatta attagaagaa caatggaacc tacacgccaa
    20341 gcaatgaaag acgctggctt aacaaactca gatatcgatg aagttatctt agttggtggg
    20401 tcaactcgta ttccagcagt acaagaagct gtcaaaaaag aaatcggtaa agaacctaac
    20461 aaaggtgtaa acccagacga agtagtggca atgggagctg caatccaagg tggcgttatc
    20521 acaggtgacg ttaaagacgt agtattatta gacgtaacac cactatcttt aggtattgaa
    20581 attttaggtg gacgtatgaa tacgttaatt gaacgtaaca ctacgattcc tacatctaaa
    20641 tctcaaatct attcaacagc agtagataat caaccatcag tagatgtaca cgtattacaa
    20701 ggtgaacgtc caatggctgc ggataataaa acacttggta gattccaatt gactgatatt
    20761 ccaccagctg aacgtggtaa acctcaaatt gaagtaacgt ttgatatcga taaaaacggt
    20821 attgtaaatg taactgcaaa agacttaggt acaaataaag aacaaagaat tacaattcaa
    20881 tcaagttctt cattatcaga cgaagaaatc gaccgtatgg taaaagatgc tgaagttaac
    20941 gctgaagcag ataaaaaacg tcgtgaagaa gtagacttaa gaaacgaagc tgacagccta
    21001 gtattccaag ttgaaaaaac tttaactgat ttaggcgaaa atatcggtga agaagataaa
    21061 aaatctgctg aagagaaaaa agacgctctt aaaactgctt tagaaggtca agatatagaa
    21121 gatattaaat ctaaaaaaga agaacttgaa aaagtgattc aagaattatc agcaaaagta
    21181 tatgagcaag cggctcaaca gcaacaacaa gcacaaggtg ctaatggtgg tcaaaacaac
    21241 gatagcactg tagaagatgc tgaatttaaa gaagtaaaag acgacgacaa aaaataatta
    21301 aacaatacaa ttagaatgtt gtcatgatct gaaaagtcaa agtcaataga acattggctt
    21361 tggcttttta tttggatagt atgacatatt acgttaaact atatgagtta attaagaaaa
    21421 ggagagatag ctgtggccaa aagagactat tatgaagttt taggcattag taaagatgct
    21481 tcaaaagatg aaatcaaaaa agcgtatcga aagctttcga aaaaatatca tccagatatt
    21541 aacaaagaag aaggtgcaga tgaaaagttt aaagaaattt ctgaagcata tgaagtttta
    21601 agtgatgata ataaacgcgc aagctatgat caatttggac atgacggtcc gcaaggtttt
    21661 ggcggacaag gattcaatgg ctctgacttt ggtggtttta gtggcttcgg tggcggcggt
    21721 tttgaagata tctttagctc tttcttcggt ggcggcagac aaagagatcc aaatgcaccg
    21781 caaaaaggtg atgatcttca gtatacaatg acattgacat ttgaagaagc ggtatttggt
    21841 acaacaaaag aaatttcaat ccgtaaagat gtaacatgcg aaacatgtca tggtgatggt
    21901 gcaaagcctg gaacaagtaa aaagacttgt agttactgta atggcgctgg tcatgtagct
    21961 gttgaacaaa atacaatttt aggtagagtt cgtactgaac aagtttgtcc taaatgtaat
    22021 ggaagtggtc aagaatttga agaagcttgt ccaacatgtc acggaaaagg tactgaaaat
    22081 aaaacagtta aattagaagt gaaagtacct gaaggtgtcg acaatgaaca acaaattaga
    22141 ttagctggtg aaggctctcc aggcgtgaat ggaggacctg ctggtgattt atacgtagta
    22201 tttagagtta aaccatctga aacatttaaa cgtgatggtg atgatattta ttacaaatta
    22261 aatgttagct tcccacaagc agctttaggc gatgaaataa aaataccaac attaaataat
    22321 gaagttatgt taacaatccc tgctggtaca caaacaggta agcaattccg cttaaaagaa
    22381 aagggtataa aaaatgttca tggctatgga tacggagatt tatatgttga tattaaagta
    22441 gtaacgccta ctaaattaac agatagacaa aaagaactaa tgaaagaatt tgcccaatta
    22501 aatggtgaag aaattaatga acaaccttcg aattttaaag atagagcaaa aagattcttt
    22561 aagggagaat aaagtatgaa ctggacagag ctttcaatta ttattaatca tgaagcagta
    22621 gaattggcta ccaatatact tgaaaatcat ggatcaaatg gtgtcgtgat agaagattca
    22681 gatgatttaa ttaaccaacc agaagataaa tacggtgaaa tttacgcttt gaaaaaagag
    22741 gattatccag ataagggagt aagattgaaa gcctatttta atgaaatgac ttatgatgat
    22801 aagttgcgac agcaaattaa agatgagtta ttaaatttag atgaacttga tcaacataac
    22861 gttcaattca gtgagcaaat tattgcagag acggattggg aaaatgaatg gaaaaactat
    22921 ttccatccat tccgagcgtc gaagaagttc acaatagttc ctagttggga aacatatgct
    22981 aaagaagcgg atgaagagct ttgcattgag ctcgacccag gtatggcttt tggaacaggc
    23041 gatcatccga ctacaagtat gtgtttgaag gcaatagaaa catatgtatt gccacagcat
    23101 tcagtaattg atgttggtac tggctcaggt atattaagta ttgcaagtca tctaatcggt
    23161 gtaaaacgaa ttaaagcgtt ggatattgat gaaatggcag tgagtgtagc taaagaaaac
    23221 ttcagaagaa atcattgtga aacgttaatt gaagctgttc caggtaactt attgaaagac
    23281 gaaacagaaa aatttgatat tgtaatagca aatattttag cgcatattat tgatgaaatg
    23341 attgaagatg cttataatac tctaaatgaa ggcggttatt ttattacttc tggtattata
    23401 aaagagaagt atgaaggtat acagtcacat atggagcgtg taggttttaa aattatttca
    23461 gaacaacatg acaacggttg ggtttgtctt gttggccaga aagtgagtga ataatgtgca
    23521 acgttatttc atagaccaaa acgctgatgt aagtcagcgt ttttttatta caaaaaaaga
    23581 agatattcat catataacaa atgtaatgag atatgatgtt ggtagtaaaa ttattttaac
    23641 ttttaatgat caaacagttt atttatgtga aattgctgat ataacaagtg ataaaattgc
    23701 agttgattta aaagaaaaac aaaatattaa tacagaattg ccagttgatg ttacgatttg
    23761 cagtggacta attaaagctg acaaatatga gtggttgcta caaaaagcta ctgaattggg
    23821 tgcttcatca tttatagctg tgagcatgga acgctcaatt gttaaattaa acgaggcgaa
    23881 agttgccaaa aagatagagc gttggcaaaa aataattaaa gaagctgctg aacaaagcta
    23941 tcgtttagtg attccatcta ttcaattcga gtcgaattta aaattaattt gtgatactat
    24001 tgataattat gactatattc ttattgcata tgaagaggaa gcaaaagatg gtgagttaag
    24061 caatttcaag caaactttac aacaattcaa ggctcaggat aaagtgttga tgatttttgg
    24121 tcctgagggt ggtttgtcag aaaatgaaat ttcattattt agtaatacta gtacagttgt
    24181 tggtcttgga ccgagaattt tacgggcaga aactgcacca ttgtatgcgt taagtgcaat
    24241 aagttatgaa aatgaattaa tggggtgaat atttatgtca acagttgcgt ttcacacatt
    24301 aggttgtaaa gtaaaccatt atgaaactga agcaatctgg caattattta aagaagcaaa
    24361 ctatgagcgc gttgactttg aagcgaatgc tgatgtattt gttattaata cttgtacagt
    24421 aacgaataca ggtgataaaa aaagtcgtca aattattaga cgtgcaataa gacaaaatcc
    24481 tgatgctgta atttgtgtaa caggttgtta tgcgcaaact tcatcagctg aaattatgga
    24541 aattcctggt gtcgatgtag tagttggtac acaagataga cataaactat taggttacat
    24601 tgatgaattc cgtaaagaac gccaaccaat taatggtgtt ggaaatatca tgaaaaatcg
    24661 taaatatgaa gaattagatg tcccatattt tacagataga acacgtgcgt cattaaaaat
    24721 tcaagaaggt tgtaacaact tctgcacatt ctgtattatt ccatgggctc gtggcttaat
    24781 gcgttcaaga gatccggaaa aagtagttga acaagcgacg caactagtga attcaggata
    24841 taaggaaatt gtattgacgg gaattcatac aggtggatat ggtcaagatt taaaagatta
    24901 taacttggcc caattattac gtgatcttga aacgattaat ggattagaac gaattcgaat
    24961 ttcttcaatt gaagcaagtc aacttacaga tgaagtaatt gacgttttag aacgttcaac
    25021 aaaagttgtg cgtcatttgc atattccatt acaatctggt tcagatacag tattaaaacg
    25081 tatgagacgt aagtatacta tggatagatt ttcagaacga ttaacaaaat tgcataaagc
    25141 tttaccagac ttggcagtta cgagtgatgt aattgttggt ttcccaggtg aaactgaagc
    25201 agagttccaa gaaacatatg attttatcgt aaagcataag ttctctgaac tgcatgtttt
    25261 cccttattct cctagaattg gcacgccagc tgcaagaatg gatgaccaaa ttgatgaaga
    25321 aattaaaaat gaacgcgtgc ataagttaat tacgctaagc aatcaactcg gaaagttata
    25381 tgcgtctaaa tttgatcaag atgtgcttga agtaattcct gaggaacagg gtgacacaga
    25441 aggtacatta gttggatatg cagataatta tatgaaagta caatttgaag gtgacgaatc
    25501 actcataggt caaattgtaa aagttaaaat tacgcaagca aattatccat taaatgaagg
    25561 gcaagcaatt aaagttgttg attttgcaac aaataaatct gatagagaag ttttagttta
    25621 agtattcctt gattcatacc ataaggttcc gtattaaaga tataggtttt gtaacgttgt
    25681 tttaacggct tcacaattta ctatataagc aactcttctc attaaataaa ttattaagag
    25741 atttttagaa caaaatctaa aagtctttgt gattttgatg tgaaatatgt ttcaaataaa
    25801 aaacaattta ctaattgacc ataaattaca gatatattat acttataaat gcatagtttt
    25861 actgtgcaat tgactataaa gttccgttga tatttggagg gagggaaata cagatgtcta
    25921 aaacagtagt acgtaaaaat gaatcacttg aagatgcgtt acgtagattt aaacgttcag
    25981 tttctaaaag tggaacaatc caagaagtac gtaaacgtga attttacgaa aaaccaagcg
    26041 taaaacgtaa aaagaaatca gaagctgcac gtaaacgtaa attcaaataa ttaatacctc
    26101 tgttgactcc ctcaacacga atattaatta tataaaacaa acatcacaag ttagtgtctg
    26161 acactaatat gtgatgtttt tttgttgtca atttttaatt aaaaaaagtt atatagttta
    26221 taaataatca aattgatatt ctataggttc ttataactat aaagtatatt caatttcatg
    26281 tataattaat gtgagggcga ggtgaaattg tgagttataa taatttttta caaatgacaa
    26341 ctatcttgga atcaacggct ggagatactt gggttgaaca agttagtaat ataattgttc
    26401 aacctatttt tacgttaata ttaacctgtt tgacattctt aggatttgta tatcaacttt
    26461 actctaaaaa aatcaatgca gctggtatta tcgccacatt atcattactt attttatttt
    26521 tgggatttct aatccaagga aatgtcaata tgcattctat cttaatattc tcaattggcg
    26581 ttatattagt tgtaattgaa ttatttgtag ttggtgcagt aattggtatt attggcatga
    26641 tactgataac tataagcata acaacgctcg gtgataattt gctatttatg cttgcgaatg
    26701 ttatcgttgc cttgatttta acgattgtag aatgggtgat attagtgaag attttcaaca
    26761 gaaagattcc gtttttggat aaagttatct taaaagattc aactaattct gagtcaggtt
    26821 acaattctca tgataaccgc tcgcacctcg taggaaagac tgctcaaaca gttacagatc
    26881 ttcgacctgc agggattatt ttttgtgaaa atgaacgtat tgatgctgtt tcagatggca
    26941 actttatttt acgcaataaa acggtaaaaa tccttgaagt tgaaggaaca agagtagttg
    27001 tgagggaagt agattaatta aaaggagcga taccatgttt agtttaagtt ttatcgtaat
    27061 agcagttatt atagtagttg cattacttat tttattctca tttgtaccga ttggtttatg
    27121 gatttcagcg ttagcagctg gcgttcatgt tggtataggt acattggttg gtatgcgttt
    27181 acgtcgtgta tctccaagaa aagttatagc gccattaatt aaagcgcata aagcaggact
    27241 agcattaaca acaaatcaat tagaatcgca ttatctagca ggaggaaatg ttgacagagt
    27301 tgttgacgct aatattgctg cacaacgtgc tgacattgat cttcctttcg aacgtgctgc
    27361 tgcaattgac cttgcaggac gtgacgtatt agaagcggtt caaatgtctg ttaatcctaa
    27421 agtcattgaa acaccattta tcgcaggtgt agcaatgaac ggtattgaag tgaaagccaa
    27481 agctcgtatc acagttagag ctaatattgc tcgacttgtt ggtggtgctg gtgaagaaac
    27541 aatcatcgca cgtgttggtg aaggtatcgt ttcaacaatt ggttctagta agcatcatac
    27601 agaagtactt gaaaacccag ataatatttc taaaacagta ttaagcaaag gtttagattc
    27661 aggtactgca tttgaaattt tatcaattga tattgctgac gttgatatta gtaaaaatat
    27721 tggtgcagac ttacaaactg aacaagcatt agcagacaaa aatattgcac aagcaaaagc
    27781 tgaagaacgt agagctatgg ctgtagcaac tgagcaagaa atgaaagcgc gtgtacaaga
    27841 aatgcatgct aaagtagttg aagccgaatc tgaagtgcca ttagctatgg ctgaagcatt
    27901 acgctcaggc aatatcagtg ttaaagatta ttataatttg aaaaatatcg aagctgatac
    27961 aggcatgaga aatgcaatta ataaacgaac tgatcaaagt gatgatgagt cacctgaaca
    28021 ttaagtcgag aggtgattaa atgagtgtcg gtattctaat ttttgtcata tcagtgatca
    28081 tttctatcat tactactatg cgcgaaaata gtcataaaga tagacaaaat caaaagccac
    28141 ctcaaaaaac atctaccgat aatgaaccaa aaaaaggtgg cttttttgaa gaaattgagc
    28201 gaacgtttaa agaaataagt gaagaattaa atgaagaaga aaagaaatca tcgaaacgaa
    28261 aatatgatga tacgttacca cctttattcg atgaacttcc aaaggaagag cctaaatcga
    28321 aacctgttgt agaacctatg gcacctaaaa aacaaccaga aacaaaaccg atgacagaga
    28381 aaccaattac agtgcctaaa gcagaaccgg tggagcagaa acatagacct tctagacaag
    28441 ataattctga cgaaattaga cgtcaattag aaaaatcact tagagatgat attaaaacga
    28501 ttcgtactga cattgataga gaaaaagaaa agcaaattgc taaaatggaa aaacgtgcta
    28561 gagatattat tgaggataaa tacttatctg aacgtacaaa acgtttgaaa ttaaagcagt
    28621 tgcttaattc tcaaaatgtc gaaaaagatt tgactaaatc agcgttccaa tttgataaag
    28681 atgaagtaat caatg
//
LOCUS       DAHHOJ010000025        26932 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.025, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000025 DAHHOJ010000000
VERSION     DAHHOJ010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 26932)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26932)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26932
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.025"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..110
                     /locus_tag="NBH32_000918"
     CDS             <1..110
                     /locus_tag="NBH32_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514540.1"
                     /translation="GILIKCLNIGGQYYLQTRMLSNVDNAEKTISGNSI"
     gene            492..752
                     /locus_tag="NBH32_000919"
     CDS             492..752
                     /locus_tag="NBH32_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514541.1"
                     /translation="MIIMRGYNERYCSWTNTAIQCDYFNKVRQVKKDGKSDRLSNNIN
                     SNIIYRVFEKCIALYNVYSRCKRALILSEHSKKNIFNIKKAV"
     gene            762..1259
                     /locus_tag="NBH32_000920"
     CDS             762..1259
                     /locus_tag="NBH32_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD1514542.1"
                     /translation="MNNRNVYDIEVSDYKGLTYKLEAFRGKVILVVNTATECIYSEQL
                     KKLETLFQKYKDRGFVVLSFPNNNFDNRQPGSNEEILKISREKFGVTFPVLAKISVNG
                     NDEHPLFTHLKNEQPGIFGSPIKWNFTKFIIDRQGNVVNRFLPMEDPLDISANIEILL
                     QESSS"
     gene            complement(1367..1455)
                     /locus_tag="NBH32_000921"
                     /pseudo
     CDS             complement(1367..1455)
                     /locus_tag="NBH32_000921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501399.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1646..3526
                     /locus_tag="NBH32_000922"
     CDS             1646..3526
                     /locus_tag="NBH32_000922"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     flavoprotein subunit"
                     /protein_id="HCD1514543.1"
                     /translation="MKLNTSNSPFTEKQVTEINNLLQTLTESQQQWLSGFLLANSSDT
                     TSDSNQQQLETEVWQQSQISEEQATSTTYMLQNKEPHIEANQRHVTVLYGSESGNAMR
                     LAEIFSERLSDIGHQVVLMSMDEYDTTNIAQLEDLFIITSTHGEGEPPDNAWDFFEFL
                     EDDNAPNLNHVRYSVLALGDQTYEFFCQAGKDVDALLENLGAERICKRVDCDIDYEED
                     AEKWMADIINMIDTTSEGIQSESVISESIKSAKEKKYSKSNPYQAEVLANINLNGTDS
                     NKETRHIEFLLDDFSESYEPGDCIVALPQNDPELVEKLISMLGWDPQSPVPINDHGDT
                     VPIVEALTSHFEFTKLTLPLLKNADIYFDNEELSERIQDESWAREYVINRDFIDLITD
                     FPTIELQPENMYQILRKLPPREYSISSSFMATPDEVHITVGTVRYQAHGRERKGVCSV
                     HFAERIKPGDIVPIYLKKNPNFKFPMKQDIPVIMIGPGTGIAPFRAYLQEREELGMTG
                     KTWLFFGDQHRSSDFLYEEEIEEWLENGNLTRVDLAFSRDQEHKEYVQHRIMEESERF
                     NEWIEQGAAIYICGDEKCMAKDVHQAIKDVLVKERHISQDEAETILRQMKQQQRYQRD
                     VY"
     gene            3592..4200
                     /locus_tag="NBH32_000923"
     CDS             3592..4200
                     /locus_tag="NBH32_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HCD1514544.1"
                     /translation="MNMPLMINLTNKNVVIVGGGVVASRRAQTLSQYVEHMTVISPTI
                     TEKLQNMVDNGVVIWKEKEFEPSDIVDAYLVIAATNEPRVNEAVKQALPEHALFNNVG
                     DASNGNVVFPSALHRDKLTISVSTDGASPKLTKSIMAELEALYPPSYSSYIDFLYTCR
                     QKIKLLDITYNEKQQLLSQIVSQEYLNHDKQAQFLAWLDVRQ"
     gene            4265..4360
                     /locus_tag="NBH32_000924"
     CDS             4265..4360
                     /locus_tag="NBH32_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514545.1"
                     /translation="MGGFVNSLIMKYLSDNDMRFKYEYYNREKDT"
     gene            4682..6052
                     /locus_tag="NBH32_000925"
     CDS             4682..6052
                     /locus_tag="NBH32_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CitMHS family transporter"
                     /protein_id="HCD1514546.1"
                     /translation="MNSDNMWLTVMGLIIIISIVGLLIAKKINPVVGMTIIPCLGAMI
                     LGYSVTDLVGFFAKGLDQVINVVIMFIFAIIFFGIMNDSGLFKPLVKRLILMTRGNVV
                     IVCAMTALIGTIAQLDGAGAVTFLLSIPALLPLYKALNMNKYLLILLLALSAAIMNMV
                     PWGGPMARVAAVLKAKSVNELWYGLIPIQIIGFILVMLFAVYLGFKEQKRIKKAIERN
                     ELPQTQDIDVHKLVEVYERDQDVRFPVKGRARTQPWIKWVNTALTLAVILAMLMNIAP
                     PEFAFMIGVSLALIINFKSVDEQMERLRAHAPNGLMMAAVIIAAGMFLGVLNETGMLK
                     AIATNLIKVIPAEVGPYLHIIVGLLGVPLDLLTSTDAYYFAVLPIVEQTAGQFGVPSV
                     STAYSMVIGNIIGTFVSPFSPALWLAIGLAEANMGTYIKYAFFWIWGFAIVMLVIAML
                     MGIVTI"
     gene            6298..8148
                     /gene="nrdD"
                     /locus_tag="NBH32_000926"
     CDS             6298..8148
                     /gene="nrdD"
                     /locus_tag="NBH32_000926"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCD1514547.1"
                     /translation="MNQIEEALTGLISKDPAIVNENANKDSDTFSTMRDLTAGIVSKS
                     YALNHLLPKHVADAHQRGDIHFHDLDYHPFQPLTNCCLIDAKNMLHNGFEIGNANVTS
                     PKSIQTASAQLVQIIANVSSSQYGGCTVDRVDELLSTYARHNEEQHRNIAKQFVKESE
                     IDRYVDQQVTKDINDAIESLEYEINTLYTSNGQTPFVTLGFGLGTDHLSRKIQQAILN
                     TRIKGLGKDRTTAIFPKLVFSIKKGTNFSPQDPNYDIKQLALKCSTKRMYPDILNYDK
                     LVEILGDFKAPMGCRSFLPSWKDAEGHFENNGRCNLGVVTLNLPRMALESAGNMTKFW
                     EIFYERIDVLHDALLYRINRLKDAVPNNAPILYKSGAFNYKLKETDDVAELFKNKRAT
                     ISMGYIGLYETATVFYGPDWETSQEAKAFTLEILKEMKRYQTKWTELYDIWFSIYSTP
                     SESLTDRFCRLDQERFGDIKDITDKGYYQNSFHYDVRKDVTPFEKLDFEKDYPYYASG
                     GFIHYCEYPKLQHNLKALEAVWDYSYDKVGYLGTNIPIDHCYECDYDGDFEATEKGFK
                     CPNCGNDNPKTVDVVKRTCGYLGNPVQRPVIKGRHKEICARVKHMKAPKE"
     gene            8145..8681
                     /gene="nrdG"
                     /locus_tag="NBH32_000927"
     CDS             8145..8681
                     /gene="nrdG"
                     /locus_tag="NBH32_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HCD1514548.1"
                     /translation="MTLLDIKQGQGYIAKIESNSFVDGEGVRCSVYVSGCPFNCVGCY
                     NKASQKFRYGEKYTDEILAEILDDCDHDYISGLSLLGGEPFCNLDITLNLVKAFRARF
                     GNTKTIWVWTGFLYEYLANDCAERRELLSYIDVLVDGLFIQHLFKPDLPYKGSLNQRI
                     IDVQQSLSHARMIEYIVS"
     gene            8781..8891
                     /locus_tag="NBH32_000928"
     CDS             8781..8891
                     /locus_tag="NBH32_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514549.1"
                     /translation="MIEYDLTIILIPILKVAYPFFKKVERKKWYFSGKEV"
     gene            complement(8907..9068)
                     /locus_tag="NBH32_000929"
     CDS             complement(8907..9068)
                     /locus_tag="NBH32_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514550.1"
                     /translation="MAIIEYLYNFSRNKQNLNSAKTTVLSYDLTNLTNFTRMAFKNIY
                     TLLTNIYLL"
     gene            9200..10822
                     /locus_tag="NBH32_000930"
     CDS             9200..10822
                     /locus_tag="NBH32_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1514551.1"
                     /translation="MKKEKVMDWTTFIGTVAVLLFAVIPMMAFPKASEDIITGINSAI
                     SDSIGSIYLFMGLAIFCFVMYIAFGKYGNVTLGKASDKPEFNTFTWAAMLFCAGIGSD
                     ILYWGVIEWAFYYQVPPNGAKSMSDEALQYATQYGMFHWGPIAWAIYVLPALPIGYLV
                     FVKKQPVYKISQACRPILKGQTDKFVGKVVDILFIFGLLGGAATSLALGVPLISAGIE
                     RLTGLDGKNMILRSAILLTITVIFAISSYTGLKKGIQKLSDINVWLSFVLLAFIFIIG
                     PTVFIMETTVTGFGNMLRDFFHMATWLEPFGGIKGRKETNFPQDWTIFYWSWWLVYAP
                     FIGLFIARISKGRRLKEVVLGTIIYGTLGCVLFFGIFGNYAVYLQISGQFNVTQYLNT
                     HGTEATIIEVVHHLPFPSLMIVLFLVSAFLFLATTFDSGSYILAAASQKKVVGEPLRA
                     NRLFWAFALCLLPFSLMLVGGERALEVLKTASILASVPLIVIFIFMMISFLIILGRDR
                     IKLETRAEKLKEVERRSLRIVQVSEEEQDDNL"
     gene            complement(10992..11555)
                     /locus_tag="NBH32_000931"
     CDS             complement(10992..11555)
                     /locus_tag="NBH32_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GbsR/MarR family transcriptional regulator"
                     /protein_id="HCD1514552.1"
                     /translation="MTRSQNKQQQLEQAKDIVINSIGQTMDLYGTNRSVGNLYGTMVF
                     EGSMTLDEMRHQLQMSKPSMSAGVKKLQEFDIVKQQFTRGSRKQHFIAEKDFFIFFRN
                     FFTKKFQREIDINVEAVKDAQAIINPLLESSDLTEAETQEAQKIKAQLDHTHVYYEWL
                     EQLTEAIESGEIFKYFPIPEQPSDSEN"
     gene            complement(11644..11739)
                     /locus_tag="NBH32_000932"
     CDS             complement(11644..11739)
                     /locus_tag="NBH32_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514553.1"
                     /translation="MKNVSIYKTTKELSFVSSLDFYLTKHLTLML"
     gene            11808..13298
                     /gene="betB"
                     /locus_tag="NBH32_000933"
     CDS             11808..13298
                     /gene="betB"
                     /locus_tag="NBH32_000933"
                     /EC_number="1.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine-aldehyde dehydrogenase"
                     /protein_id="HCD1514554.1"
                     /translation="MELLKHLSQRQYIDGEWVESANKNTRDIINPYNQEVIFTVSEGT
                     KEDAERAILAARRAFESGEWSQETAETRGKKVRAIADKIKEHREALARLETLDTGKTL
                     EESYADMDDIHNVFMYFAGLADKDGGEMIDSPIPDTESKIVKEPVGVVTQITPWNYPL
                     LQASWKIAPALATGCSLVMKPSEITPLTTIRVFELMEEVGFPKGTINLILGAGSEVGD
                     VMSGHKEVDLVSFTGGIETGKHIMKNAANNVTNIALELGGKNPNIIFDDADFELAVDQ
                     ALNGGYFHAGQVCSAGSRILVQNSIKDKFEQALIDRVKKIKLGNGFDADTEMGPVIST
                     EHRNKIESYMDVAKAEGATIAVGGKRPDRDDLKDGLFFEPTVITNCDTSMRIVQEEVF
                     GPVVTVEGFETEQEAIQLANDSIYGLAGAVFSRDIGKAQRVANKLKLGTVWINDFHPY
                     FAQAPWGGYKQSGIGRELGKEGLEEYLVSKHILTNTNPQLVNWFSK"
     gene            13559..15268
                     /gene="betA"
                     /locus_tag="NBH32_000934"
     CDS             13559..15268
                     /gene="betA"
                     /locus_tag="NBH32_000934"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HCD1514555.1"
                     /translation="MSNKNKSYDYVIIGGGSAGSVLGNRLSEDKDKEVLVLEAGRSDY
                     FWDLFIQMPAALMFPSGNKFYDWIYSTDEEPHMGGRKVAHARGKVLGGSSSINGMIYQ
                     RGNPMDYEGWAEPEGMETWDFAHCLPYFKKLEKTYGAAPYDKFRGHDGPIKLKRGPAT
                     NPLFQSFFDAGVEAGYHKTPDVNGFRQEGFGPFDSQVHRGRRMSASRAYLHPAMKRKN
                     LTVETRAFVTEIHYEGRRATGVTYKKNGKLHTIDANEVILSGGAFNTPQLLQLSGIGD
                     SEFLKSKGIEPRVHLPGVGENFEDHLEVYIQHKCKEPVSLQPSLDIKRMPFIGLQWIF
                     TRKGAAASNHFEGGGFVRSNNEVDYPNLMFHFLPIAVRYDGQKAAVAHGYQVHVGPMY
                     SNSRGSLKIKSKDPFEKPSIRFNYLSTEEDKKEWVEAIRVARNILSQKAMDPFNGGEI
                     SPGPEVQTDEEILDWVRRDGETALHPSCSAKMGPASDPMAVVDPLTMKVHGMENLRVV
                     DASAMPRTTNGNIHAPVLMLAEKAADIIRGRKPLEPQYIDYYKHGVHDENEGAIEVKP
                     YAK"
     gene            15545..15757
                     /locus_tag="NBH32_000935"
     CDS             15545..15757
                     /locus_tag="NBH32_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1514556.1"
                     /translation="MSFYEYIQTFKDDKTPLGELAIWIKEDDSFPKQEKLTENILSYF
                     HQMSNIDHEFLEIVKRSLSLYDQLKS"
     gene            16037..16480
                     /locus_tag="NBH32_000936"
     CDS             16037..16480
                     /locus_tag="NBH32_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCD1514557.1"
                     /translation="MNIYDEYRSYVIEELDDCLTIQKNNDTAYYDVLEAINDLSNDSL
                     CVLNHLYINEGQEETFEQKFLRRNKHLKNVDGFKALRFLRPRTAGRHYIIITLWENRK
                     AFYHWQNSAEYKHTHKHRGTSKGADVKIINRELSYNIRIELADMV"
     gene            16674..18272
                     /locus_tag="NBH32_000937"
     CDS             16674..18272
                     /locus_tag="NBH32_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl--CoA ligase"
                     /protein_id="HCD1514558.1"
                     /translation="MQKSALFAPEKYNIISEIEKYSHIPDKKAILYHNTEGEDISVTY
                     QQLIEQSNKVGNVLASHGLSKGDKVLIMMPRSIATYELYIAALKLGVAIIPCSEMLRT
                     KDLQYRITHGEINAVIALEPFTVEFEKIKGYDALTKFVIAGQKDGWISLEAEKENASN
                     KLEMADTTRDDTAILSYTSGTTGNPKAVTHCHGWGFAHLQMAPKHWLCINEDDLVWAT
                     AAPGWQKWVWSPFLSVLGSGATAFVFNGRFSPETYLELLQKYQINVLCCTPTEYRMMA
                     KLQNLNDYDLTHLHSAVSAGEPLNREVVEQFKKYFNLTVRDGYGQTESTLLIGFLKDT
                     PQRIGSMGKGIPGSSVTVVDDEGNSVPANTEGNIAVPLDLPALFKGYYKEPERTAKAQ
                     TGKYYITGDLAHVDDDGYFWFEGRRDDIIISSGYTIGPFEVEDALTNHPAVKECAVVA
                     KPHDIRGNIVKAFVILQDHTAGDDTLVKELQQFVKNEVAPYKYPREIEFVDDLPKTNS
                     GKIRRVELRDAEIEKWQQQKDSNQ"
     gene            complement(18332..18663)
                     /locus_tag="NBH32_000938"
                     /pseudo
     CDS             complement(18332..18663)
                     /locus_tag="NBH32_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501381.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            18959..20455
                     /gene="mqo"
                     /locus_tag="NBH32_000939"
     CDS             18959..20455
                     /gene="mqo"
                     /locus_tag="NBH32_000939"
                     /EC_number="1.1.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase (quinone)"
                     /protein_id="HCD1514559.1"
                     /translation="MAKSNSKDIVLIGAGVLSTTFGSMLKEIEPDWNIHVYERLDRPA
                     IESSNERNNAGTGHAALCELNYTVLQPDGSIDIEKAKVINEEFEISKQFWGHLVKSGS
                     IENPREFINPLPHISYVRGKNNVKFLKDRYEAMKAFPMFDNIEYTEDIEVMKKWIPLM
                     MKGREDNPGIMAASKIDEGTDVNFGELTRKMAKSIEAHPNATVQFNHEVVDFEQLSNG
                     QWEVTVKNRLTGEKFKQVTDYVFIGAGGGAIPLLQKTGIPESKHLGGFPISGQFLACT
                     NPQVIEQHDAKVYGKEPPGTPPMTVPHLDTRYIDGQRTLLFGPFANVGPKFLKNGSNL
                     DLFKSVKTYNITTLLAAAVKNLPLIKYSFDQVIMTKEGCMNHLRTFYPEARNEDWQLY
                     TAGKRVQVIKDTPEHGKGFIQFGTEVVNSQDHTVIALLGESPGASTSVSVALEVLERN
                     FPEYKTEWAPKIKKMIPSYGESLIEDEKLMRKIRKQTSKDLELGYYEN"
     gene            complement(20647..21537)
                     /locus_tag="NBH32_000940"
     CDS             complement(20647..21537)
                     /locus_tag="NBH32_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002478556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose bisphosphate aldolase"
                     /protein_id="HCD1514560.1"
                     /translation="MNKEQLEKMKNGKGFIAALDQSGGSTPKALKEYGVNEDQYSNED
                     EMFQLVHDMRTRVVTSPSFSPDKILGAILFEQTMDREVEGKYTADYLADKGVVPFLKV
                     DKGLAEEQNGVQLMKPIDNLDSLLDRANERHIFGTKMRSNILELNEQGIKDVVEQQFE
                     VAKQIIAKGLVPIIEPEVNINAKDKAEIEKVLKAELKKGLDSLNADQLVMLKLTIPTE
                     PNLYKELAEHPNVVRVVVLSGGYSREKANELLKDNAELIASFSRALASDLRADQSKEE
                     FDKALGDAVESIYDASVNKN"
     gene            complement(21660..22076)
                     /locus_tag="NBH32_000941"
     CDS             complement(21660..22076)
                     /locus_tag="NBH32_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514561.1"
                     /translation="MRLKVLFHFIAAIFISFMLLWMTMLFDLISNQSHLKALLLNLDF
                     LIPSDNTPYILEIICHLLIGSVIYFIFVLLFHTSKRLYYLCYIPLFFLFIALYPFLVF
                     IAQRPIFQFSVTELIGWIITHIFFMSLIALVIPRIK"
     gene            22334..23671
                     /locus_tag="NBH32_000942"
     CDS             22334..23671
                     /locus_tag="NBH32_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HCD1514562.1"
                     /translation="MSKAHQLIQEDEHYFAKSGRIKYYPLVIDHGYGATLVDIEGKTY
                     IDLLSSASSQNVGHAPREVTEAIKAQVDKFIHYTPAYMYHEPLVRLAKKLCQIAPGDF
                     EKRVTFGLTGSDANDGIIKFARAYTGRPYIISFTNAYHGSTFGSLSMSAISLNMRKHY
                     GPLLNGFYHIPFPDKYRGMYEQPQANSVEEYLAPLKEMFAKYVPADEVACIVIETIQG
                     DGGLLEPVPGYFETLEKICREHGILIAVDDIQQGFGRTGTWSSVSHFNFTPDLITFGK
                     SLAGGMPMSAIVGRKEIMNCLEAPAHLFTTGANPVSCEAALATIQMIEDQSLLQASAE
                     KGEYVRKRMDQWVSKYNSVGDVRGKGLSIGIDIVSDKKLKTRDASAALKICNYCFEHG
                     VVIIAVAGNVLRFQPPLVITYEQLDTALNTIEDALTALEAGNLDQYDISGQGW"
     gene            complement(23751..25199)
                     /locus_tag="NBH32_000943"
     CDS             complement(23751..25199)
                     /locus_tag="NBH32_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1514563.1"
                     /translation="MGSFFNKIARKEDPAIYQNKDGHLKRTLRVRDFLALGVGTIVST
                     SIFTLPGIVAAQHAGPAVALSFLLAAIVAGLVAFTYAEMAAAMPFAGSAYSWVNVLFG
                     EFFGWVAGWALLAEYFIAVAFVASGFSANLRGLVKPIGIELPAALSNPFGTNGGFIDI
                     IAAIVILLTALLLSRGMSEAARMENILVILKVLAIILFVIVGLTAINVSNYVPFIPEH
                     KVTATGDFGGWQGIYAGVSMIFLAYIGFDSIAANSAEALDPQKTMPRGILGSLSVAIV
                     LFIAVALVLVGMFHYSQYANNAEPVGWALRQSGHGVVAAIVQAISVIGMFTALIGMML
                     AGSRLLYSFGRDGLLPSWLSHLNDKHLPNRALVILTIIGVLIGSMFPFAFLAQLISAG
                     TLVAFMFVSLAMYRLRKREGKDLPIPAFKLPLYPVLPAVTFVLVLLVFWGLGFEAKLY
                     TLIWFIVGIILYLSYGLRHSKKNDVAEYHPPK"
     gene            25886..26845
                     /locus_tag="NBH32_000944"
     CDS             25886..26845
                     /locus_tag="NBH32_000944"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HCD1514564.1"
                     /translation="MKTFGKKVVLIGDGSVGSSYAFAMVTQGVADEFVIIDIAKDKVK
                     ADVQDLNHGTVHSPSPVDVKAGEYEDCKDADLVVITAGAPQKPGETRLQLVEKNTKIM
                     KSIVKSVMDSGFDGYFLIAANPVDILTRFVKEYTGLPAERVIGSGTVLDSARLQYLIS
                     QELGVAPSSVDASIIGEHGDTELAVWSQANVAGISVYDTLKEQTGSEAKAEEIYVNTR
                     DAAYEIIQAKGSTYYGIALALMRISKAILNNENNVLNVSIQLDGQYGGHKGVYLGVPT
                     LVNQHGAVKIYEMPLSAEEQALFDKSVKTLEDTFDSIKYLLED"
BASE COUNT         9442 a         4029 c         5104 g         8357 t
ORIGIN      
        1 atggcatact tataaagtgt ctaaatattg gtggtcaata ttacttacaa acacgaatgt
       61 taagtaatgt agacaatgct gaaaaaacaa taagtggaaa ctctatatag aatgttcgtt
      121 tttattttta ataattgaat aatcattatt gatgatttaa tatgtattcg gtcgcacacg
      181 atacaaagtg acttgtttta atgatatgca ataatatgac gtttatcgtc gcatgtaaca
      241 atgtatgagt gtgtcagttt aattttaaaa gggttataag gatacttaat aagataaagg
      301 agggcttaat aaacaatcac atgtgaagtt tttaaggatt atatgagttg ggcagctctt
      361 taatccatag ttataacgaa ttttaaattg ttagatttaa cgtatggtat cagtatgtat
      421 gagtttaact taagtattaa tctatagttt aaagagcggt ctaattgtct ttttaaaaat
      481 agaaaataag gatgattata atgaggggtt ataacgaaag atattgtagc tggactaata
      541 cagctataca atgtgattat tttaataaag taagacaggt gaaaaaagac ggtaaatcgg
      601 ataggttgag caacaacata aactcgaaca ttatttatcg tgtgtttgaa aaatgtatcg
      661 cgctatataa tgtgtattcg cggtgtaaac gcgcacttat tttatcagaa cattctaaaa
      721 agaacatatt caatataaaa aaggctgtgt aggggtgtct tatgaataat agaaatgtgt
      781 atgatatcga agtaagtgat tataaaggct taacttataa attagaagca tttagaggta
      841 aagtgatttt agttgttaat actgcaacag aatgtatata tagcgaacaa ttgaaaaaac
      901 tagagacttt gtttcaaaaa tataaggatc gtgggtttgt agtgttgagt tttccgaata
      961 ataattttga caatcgacaa ccaggttcta atgaagaaat cttgaaaatc agtcgtgaga
     1021 aatttggtgt tacatttcca gtgctagcta aaatatctgt gaacgggaac gatgaacatc
     1081 cgttatttac gcatttaaag aatgaacaac caggaatctt tgggtcccca ataaaatgga
     1141 atttcacaaa atttataatc gatcgacaag gcaatgttgt aaatcgattc ttgccaatgg
     1201 aagatccatt ggatatatcg gcaaatatag aaatattatt gcaagaatct tcatcttaaa
     1261 tttaaaattg agcgcttagt atgcaaataa cacaagagcc tcttaatgat gtactaatag
     1321 tatgtcatta ggaggcattt tatatttaga aaatcagtaa tttgacctat tttactgaaa
     1381 ggctgatttg gtacataata aatagtaaat aaaaaataac aacgatgcat tactgtatgc
     1441 attaataact gacacgacat aatcaaagtt ttgtctgaat tactgtgtca ttcaagcaac
     1501 atgaaggttg taaatatttg taagtataac tatcagacac aactcaagtt tgttttaatt
     1561 tataccatac atattaaaaa attgactaaa ctatcgataa aaaatgagtc aatcatctat
     1621 ttcaagttta ttgaggtgaa aatatttgaa attaaataca tctaatagtc catttacaga
     1681 aaaacaagtt acagagatta acaatctgct gcaaacatta acagagagcc aacaacaatg
     1741 gcttagtggg tttttactag ctaatagtag cgatacgaca agtgatagta atcaacaaca
     1801 attagagaca gaagtgtggc aacaatcaca aatatcagaa gaacaagcaa cttcaacaac
     1861 gtatatgtta caaaataaag agccacatat cgaagctaat cagcgtcatg tcactgtgct
     1921 atacggttct gaatcaggta atgccatgcg attggctgaa atattttcag aacgattaag
     1981 tgatatcgga catcaagttg ttttgatgtc aatggatgaa tatgatacga caaacatcgc
     2041 acagttagaa gatttattta ttattacgtc tactcatggt gaaggagaac cgcccgataa
     2101 tgcatgggat ttctttgaat ttttagaaga cgataacgca cctaatttaa atcatgtgag
     2161 atattcagta ctagctttag gtgatcaaac atatgaattt ttctgtcaag ccggtaaaga
     2221 tgtagatgct ttactagaaa atctaggcgc tgagcgtata tgtaagcgtg tagattgtga
     2281 tattgattat gaagaagacg cagaaaagtg gatggcagac atcattaata tgattgatac
     2341 cacatcagaa ggtattcaaa gtgaatcggt gataagtgaa tcaattaagt ctgccaaaga
     2401 aaagaaatat tctaaatcaa atccatacca agcagaagtt ttagcgaata tcaatttaaa
     2461 tggtaccgat tcaaataaag aaacacgaca tatagaattt ttacttgatg attttagtga
     2521 atcatatgaa ccaggagatt gtatagtagc attaccgcaa aacgaccctg aattggttga
     2581 aaaactaata tccatgttag gttgggatcc gcaatctccg gtgccaatta atgatcatgg
     2641 tgatacagtt cctattgttg aagcactaac atcacatttt gaatttacta aattaacatt
     2701 gccattattg aaaaatgcag atatctattt tgacaatgaa gaattatctg aacgtattca
     2761 agatgagtca tgggcgcgtg aatatgttat aaatcgggac tttatagatt taataacaga
     2821 ttttccaact atagaattac aacctgagaa tatgtatcaa atccttagaa aattaccacc
     2881 aagagagtat tcgatttcta gtagttttat ggcaacgcca gatgaagtgc atattaccgt
     2941 tggtacggtt cgttatcaag cacatggacg tgagagaaaa ggtgtatgct cggttcattt
     3001 tgctgagcgt attaaaccag gcgatatagt accaatttat ttgaagaaaa atccgaactt
     3061 caaatttccg atgaagcaag atataccggt tattatgatt ggaccaggta ctggaattgc
     3121 tccttttaga gcatatttac aagaacgtga agaacttggt atgactggaa aaacatggtt
     3181 gttctttggt gatcaacacc gtagttctga ctttttatat gaagaagaaa tagaagaatg
     3241 gcttgaaaat ggaaacttaa cacgcgtaga tttagcattt tcaagagacc aagaacacaa
     3301 agaatatgta cagcatcgta taatggaaga aagtgaacgt ttcaatgaat ggattgagca
     3361 aggcgcagca atctatattt gtggcgatga aaaatgtatg gcgaaggatg tccatcaagc
     3421 cattaaagat gtattggtaa aagaacgtca tatttctcaa gacgaagctg agacgatatt
     3481 gcgtcaaatg aaacaacaac aacgctatca acgtgatgtt tattaatgct tcaatgtgtt
     3541 aaatatttta aggtgtaatg atgtaaaatt atataaagga tgttgctcaa catgaatatg
     3601 ccattaatga taaatttaac aaataaaaat gtcgtcatag ttggtggagg cgtcgttgca
     3661 agtcgtcggg cacaaacatt aagtcaatac gttgaacata tgacggtcat cagtccgaca
     3721 atcactgaaa aacttcaaaa tatggtagat aacggtgtcg tcatatggaa agaaaaagag
     3781 tttgaaccaa gcgatattgt agacgcgtat ctagttattg cagcaaccaa tgagccacgt
     3841 gtcaatgaag cggtaaaaca agccttacct gagcatgccc tttttaataa tgttggagat
     3901 gcatcaaatg gcaatgttgt atttccaagt gcactacacc gcgacaagct aactatcagt
     3961 gtatcaactg atggtgcgag tcctaagttg acaaaatcaa ttatggcaga gcttgaggcg
     4021 ttatatccac catcatacag ttcgtatatc gactttttat atacttgccg acagaaaata
     4081 aagttactag atataacata taacgaaaag caacagttac tgtcacaaat tgtgtcacaa
     4141 gaatatttaa atcatgacaa acaagctcaa tttttagcgt ggttggatgt aagacaataa
     4201 tagcggaccg tctaaggtaa gtcttcttat tttaacttta acgcttaatc attgaaatta
     4261 agacatgggc ggctttgtga atagtctaat aatgaagtat ttaagcgata atgatatgcg
     4321 ttttaaatat gaatattaca atagagaaaa agatacgtag aacaaactta ataaaataga
     4381 tggataaatt gaaatctggt tgaagtcgtt actatcatag cgacctttag ccagattttt
     4441 tgtgcaatag aaagcaataa taaaaatgat aaatcaaaat gaaatacagg acaggatata
     4501 caaggattag tcatgccatg ttatcaagta ggaaaatcaa acttcactat tgatagttac
     4561 acaaaaaaga aatttttgat aaaatgagtt aacttaaata taaaaaatta tattaattat
     4621 tatatttaag ttaaagaggg ggattatgta aattgtatta aaagtggagg gagaaaataa
     4681 tatgaatagt gataatatgt ggttaacagt aatggggctc attattatta tttcaattgt
     4741 aggtttactc attgccaaaa agataaatcc agttgtaggt atgacaatca taccttgctt
     4801 aggggcaatg attttaggat atagtgtgac agatttggtt ggattttttg ctaaaggatt
     4861 agatcaagtc atcaacgttg ttattatgtt tatctttgcc attattttct ttggcatcat
     4921 gaatgatagt ggtttattca agccgcttgt caaacgctta atattaatga cacgaggcaa
     4981 tgtcgtcatt gtctgtgcaa tgacagcttt aattggcaca atagcccaat tagatggggc
     5041 cggtgcggta acatttttgc tttctattcc tgcattatta cctttatata aagcgttaaa
     5101 tatgaataaa tatttattga ttttactatt agcattaagc gcggcgatta tgaacatggt
     5161 accttgggga ggtccaatgg ctcgtgtagc tgcagtgtta aaagccaaaa gtgtcaatga
     5221 attatggtat ggattaatac ctattcaaat aataggtttc attcttgtta tgttatttgc
     5281 ggtatatctt ggatttaaag aacaaaaacg tatcaaaaaa gcaatagaga gaaatgaatt
     5341 accgcaaaca caagatatag atgtacataa attagttgaa gtatatgaac gagatcaaga
     5401 tgtaaggttt cctgtaaaag gacgtgcacg aacacaacca tggataaaat gggtgaatac
     5461 agctttaact ttagctgtta ttctagcgat gttaatgaat attgcgccac ctgaatttgc
     5521 attcatgata ggtgtttcat tggcacttat tattaatttt aaatcagtgg atgaacaaat
     5581 ggagcgatta agagcgcatg cgccgaatgg attaatgatg gctgcagtga ttattgcagc
     5641 aggtatgttt ttaggtgtac taaatgaaac cggtatgctt aaagcgattg cgacgaattt
     5701 aatcaaagtg attcctgcag aagtagggcc atacttgcat attattgtag gtttacttgg
     5761 tgtaccatta gatttactaa ctagcacaga cgcttattat tttgctgtgt taccaatcgt
     5821 cgaacaaaca gcagggcaat ttggtgtacc gtctgtatca acagcttatt caatggtcat
     5881 agggaatatt ataggtacat ttgtcagccc gttttcacca gccttatggt tggcaattgg
     5941 tttagcagag gcaaacatgg gcacgtatat taagtatgca ttcttttgga tttggggatt
     6001 cgccatcgtg atgttagtaa ttgcgatgtt gatgggcatt gtgacgattt aagtatgaaa
     6061 aaatagaaac tatggtcacg ttgcaaaatg aaatgatggt atgtaactat gctagaaaga
     6121 cggaacgatt cgagaacaat tttaaaaatt aatgaaataa ttgtgtagaa atatgaattt
     6181 cactaaatgt taataacttt gtgacgtttt agttaacaga ctaataaaaa tttgaaaata
     6241 ctatatatag tggtataacg taatgagtag acacaatata taggaagaag gggtaaaatg
     6301 aatcaaatcg aagaagcatt aacgggtttg atttctaaag atcctgctat tgttaacgaa
     6361 aatgccaaca aagatagtga tacattttca acaatgagag atttaacagc aggtatcgtt
     6421 tctaaatctt acgcattaaa tcatttatta ccaaagcacg ttgcagatgc acatcaaaga
     6481 ggggacatac attttcacga cttagattat catccattcc aaccgttaac aaactgttgt
     6541 ttaatagatg ctaaaaatat gctacataat ggatttgaaa taggcaacgc gaatgtaact
     6601 tcaccaaaat caatacaaac tgcatcagcg cagcttgtac aaattatagc caatgtttct
     6661 agcagtcaat atggtggctg tacggttgac cgcgttgacg aattacttag tacatatgca
     6721 cgacataatg aagaacaaca taggaatatc gcaaagcaat ttgtcaaaga atctgaaatc
     6781 gaccgttatg ttgatcaaca agtcactaaa gacatcaatg atgcgattga aagtttagaa
     6841 tatgaaatta ataccttata tacatctaat ggacagacac cttttgtaac attaggattc
     6901 ggcttaggta cagatcattt aagtcgcaaa attcaacaag ctatcttaaa tactcgtatc
     6961 aaaggcttag gaaaagaccg cacgacagcg attttcccaa aacttgtatt ttcaattaaa
     7021 aaaggaacca actttagtcc gcaagatccg aactatgaca ttaaacaact agcattaaag
     7081 tgttcaacaa aacgtatgta tccagatatt ttaaattatg acaaactcgt agaaatatta
     7141 ggtgatttca aagcgccaat gggttgtcgt tcatttttac caagttggaa agatgcggaa
     7201 ggtcattttg aaaataatgg tcgttgtaac cttggtgttg taacgcttaa tttacctaga
     7261 atggcattag aatctgctgg taatatgaca aaattctggg aaatctttta tgaacgtatc
     7321 gatgtgttac atgatgcatt actttatcgt ataaatcgtt tgaaagatgc tgtaccgaat
     7381 aacgcaccga ttttatataa aagtggcgca tttaactata aattaaaaga aacagatgat
     7441 gttgctgagt tatttaaaaa taaacgtgca acgatttcaa tgggctatat agggttgtat
     7501 gaaacagcta ctgttttcta tggtccagac tgggaaacat ctcaagaagc aaaagcattt
     7561 acgcttgaaa ttcttaaaga aatgaaacgt tatcaaacga aatggacaga attatatgac
     7621 atttggttca gtatttacag tacgccgagt gaatcgctaa cggatcgttt ttgtcgttta
     7681 gaccaagaga gatttggaga tattaaagac attacagata aaggatatta ccaaaactct
     7741 ttccattatg atgtacgtaa agatgttaca ccttttgaaa agttagattt tgaaaaagat
     7801 tatccttatt atgcgagtgg tggtttcatt cactattgtg agtatccgaa attgcaacac
     7861 aatttgaaag cactagaagc ggtatgggac tactcttatg acaaagttgg ttacttaggt
     7921 acaaatattc cgattgatca ttgttatgaa tgtgattacg atggagattt tgaagcaact
     7981 gaaaaaggat ttaaatgccc gaactgtggc aatgataatc ctaaaacagt tgatgtcgtt
     8041 aaacgaacat gtggttactt aggcaatcca gttcaacgtc cagtaattaa aggccgtcat
     8101 aaagaaattt gcgcacgagt aaaacatatg aaagcgccta aagaatgaca cttttagaca
     8161 ttaaacaagg acaaggttat attgctaaaa tagaatcaaa tagctttgtt gacggtgaag
     8221 gagtaagatg cagtgtttat gtatcaggat gtccatttaa ttgtgttgga tgttacaaca
     8281 aagcctcaca aaagttcaga tatggcgaga aatacactga tgaaatatta gcagaaatat
     8341 tagatgactg cgatcatgat tatatatctg ggctaagtct attaggtggc gaaccatttt
     8401 gtaatttgga tattacatta aatcttgtca aagcatttcg agcacgtttt ggaaatacaa
     8461 agacaatttg ggtatggact ggatttttat atgaatattt agcaaatgat tgtgcagaac
     8521 gtcgagagtt attatcatac attgacgttt tagtagatgg gctatttata caacacttat
     8581 tcaaacctga tttaccatat aaaggttctt taaatcaacg tattatagat gtacaacaat
     8641 cactctcgca tgcgcgtatg attgaatata ttgttagttg aatatgtatt agaagtcaag
     8701 gtaacatgcg ttgccttggc ttctttttag gttaggtaca taattgaaag ttaataaaag
     8761 caatttttta gaaaaatata ttgatagaat atgacctaac aatcattttg ataccaatac
     8821 taaaagttgc atatccgttt tttaaaaaag ttgaaagaaa aaagtggtat tttagtggga
     8881 aggaagtcta actttttggt agcgttttac aataaataaa tattcgttaa taacgtataa
     8941 atattcttaa atgccattct agtaaaattt gttaaattcg ttaaatcgta acttaacact
     9001 gttgttttag cgctattaag gttttgttta ttacgggaaa aattatataa atattcaata
     9061 attgccaagt ttcaaattgt atgaaatttg cattattatt aaatgttagt tattgtcaat
     9121 tttgtgaatc aatataatta ttacattttg agataaatcg aaacaggatt cataaaatta
     9181 ataattaggg ggagcacaat tgaaaaaaga gaaagttatg gactggacga cctttatagg
     9241 gacagtagct gtacttcttt ttgcagttat acctatgatg gcttttccaa aagcaagtga
     9301 agatatcatc actggtatta atagtgccat ttctgattca attggttcga tatatttatt
     9361 tatggggctg gcgatttttt gctttgtaat gtatatcgca tttggtaaat atggtaatgt
     9421 cacacttggt aaagcaagtg ataaaccaga atttaataca tttacatggg ctgcaatgct
     9481 gttttgtgca ggcataggct ctgatatttt atactggggc gttattgaat gggcttttta
     9541 ctatcaagtt ccaccaaatg gcgcgaaaag tatgagtgat gaagcactcc aatatgcgac
     9601 gcaatatggt atgttccact gggggccaat tgcttgggct atttatgttc taccagcatt
     9661 accaattggt tatttagtat ttgttaaaaa acaaccggtg tataaaatta gtcaagcttg
     9721 tcgtccgatt ttaaaaggtc aaacagataa atttgtaggt aaagttgtag atatcttatt
     9781 tatctttgga ttgctaggtg gtgcggcaac atcactagcg ttaggtgtgc cattaatttc
     9841 tgcaggcata gaaagattaa ctggtttaga tggtaaaaat atgattttac gttcggccat
     9901 tttattaaca atcacggtta tatttgccat tagttcatat acaggattga aaaaaggtat
     9961 tcaaaagtta agtgatatca acgtttggct atcatttgta cttttagcct ttatatttat
    10021 tattggaccg actgttttta ttatggaaac gacagtgaca gggttcggaa atatgttgag
    10081 agattttttc catatggcaa catggttaga accattcggt ggtattaaag gtcgaaaaga
    10141 aacgaatttc ccacaagact ggacaatatt ctactggtca tggtggttag tatatgcgcc
    10201 atttatcggt ttatttatcg ctagaatttc aaaaggtcga cgccttaaag aagtcgtgct
    10261 aggaacaatt atttatggaa cgcttggatg cgtattattc tttggtattt ttggtaacta
    10321 tgctgtgtat ttacaaattt ctggacagtt taatgtaaca caatatttaa atacacatgg
    10381 tacagaggca accattattg aagtggtgca tcatttacca ttcccatcat tgatgattgt
    10441 actattctta gtatctgctt tcttattctt agcaacaaca tttgattcgg gttcatatat
    10501 tttagcggca gcatctcaga aaaaagtggt aggcgaacca ttacgtgcca atcgtttatt
    10561 ctgggcattt gcattgtgct tattgccatt ttcattgatg ctagttggtg gcgaacgtgc
    10621 attagaagta ttgaaaactg cttcaatact ggcaagtgtg ccattaattg ttatttttat
    10681 tttcatgatg atatcatttt taatcatttt agggcgcgat agaattaaac ttgaaacgcg
    10741 tgctgaaaaa ttaaaagaag ttgaacgtcg ttcattgcga atcgttcaag tatcagaaga
    10801 agaacaagac gataatttat aattaaataa atagcacata agactttgat ttttaaaagt
    10861 aaataattga ttgtcatata aaacaaaaac ggtaatttct atttattgag atagaaatta
    10921 ccgtttattt cgtggacctg ttgcattgtt ttcatcatgc ataatcatca ttgtcgttgt
    10981 ttgagtcaat tttaattttc agaatcagaa ggttgttctg gaattgggaa atatttgaaa
    11041 atttcaccgc tttcaatcgc ttcggttaac tgttctaacc attcgtaata aacatgtgta
    11101 tgatcaagct gagctttaat tttttgtgcc tcttgtgttt cagcttcagt taaatcactg
    11161 ctttcaagta atggattgat aatagcttga gcatctttta ctgcttcgac attgatatca
    11221 atttcacgct ggaatttttt agtgaaaaag tttcggaaaa agatgaaaaa gtctttctcg
    11281 gcgataaaat gttgtttgcg gcttcctctc gtaaattgtt gtttaacaat atcaaattcc
    11341 tgcaatttct taacgccagc actcatactt ggtttgctca tttgcaattg atgacgcatt
    11401 tcatcaagcg tcatactgcc ttcaaacacc atagtgccat ataagtttcc tacacttcta
    11461 ttagtgccat acaaatccat tgtctgtcca attgaattaa ttacaatatc ttttgcttgt
    11521 tctaattgtt gctgtttgtt ctgagaacga gtcatcattg cacctccgta catcattttg
    11581 gtcacgttaa aataaatact aatacattat aaaacctttt ctaaaaaaag acattaaaaa
    11641 tatttaaagc attaaagtta aatgtttcgt taaataaaaa tctaacgaac ttacaaaact
    11701 taattcttta gttgttttgt aaattgacac atttttcatt tctatgctaa cataagtttg
    11761 taaaattcgt taaataaaaa tttaacaaac ttaacggagg ttgttgaatg gaacttttaa
    11821 aacatttatc tcagcgtcaa tatattgatg gtgagtgggt tgaaagcgcg aataaaaata
    11881 caagagatat tatcaatcct tacaatcaag aagtgatatt tacggtttct gaagggacaa
    11941 aagaggatgc agaacgtgca atcttagctg caagacgtgc gtttgagtct ggtgaatggt
    12001 cgcaagaaac tgctgaaaca agaggtaaaa aagtacgtgc catcgcggac aagattaaag
    12061 aacatcgcga agcgttagca cgattagaaa cattagatac tggaaaaacg ttagaagaat
    12121 catatgcaga tatggatgat attcataatg tgtttatgta ttttgctgga ttagcagata
    12181 aagacggtgg cgaaatgatt gattcaccaa ttccagatac agaaagcaaa attgttaaag
    12241 aaccagtagg tgtagttaca caaattacac cttggaatta tccgttatta caagcatcat
    12301 ggaaaattgc gcctgcgctt gctacgggtt gttcactagt tatgaaacca agtgaaatta
    12361 caccattaac aacaatacgt gtttttgaat taatggaaga agttggtttc cctaaaggaa
    12421 caattaatct tattctaggt gcaggttctg aagttggtga cgtaatgtca ggtcataaag
    12481 aggttgacct tgtatcattt acaggtggca ttgagactgg taagcatatt atgaaaaatg
    12541 ctgctaataa tgttacgaat attgccttgg aacttggcgg taaaaatcca aacattatct
    12601 ttgatgatgc tgattttgaa ttggcggtag accaagcgtt aaatggtgga tatttccatg
    12661 caggtcaagt ttgttcagca ggatcaagaa tattagtaca aaacagtatt aaagacaaat
    12721 ttgagcaagc acttattgat cgcgtgaaaa aaattaaatt aggtaatggt tttgatgctg
    12781 atactgaaat gggaccagtg atttcaacag aacatcgtaa taagatcgaa tcttatatgg
    12841 atgtagctaa agcagaaggc gcaacaattg ctgttggtgg taaacgtcca gatagagatg
    12901 atttaaaaga tggtctattc ttcgagccaa cagtcattac aaattgtgat acgtcaatgc
    12961 gtattgtaca agaagaggtt ttcggacctg tcgttactgt agaaggcttt gaaactgaac
    13021 aagaagcgat tcaattagcg aatgattcta tatatggttt agcaggtgct gtattttcta
    13081 gagatattgg aaaagcacaa cgcgttgcta acaagttgaa acttggaacg gtgtggatta
    13141 atgatttcca tccatatttt gcacaagcgc catggggtgg atacaaacaa tcaggtatcg
    13201 gtagagaatt aggcaaagaa ggcttagaag agtaccttgt ttcaaaacac attttaacaa
    13261 atacaaatcc acaattagtg aattggttta gcaaataaaa attagataag gtgagtgcca
    13321 ttgtaagaac acaagacact cactttgttt tgtataagtg gcgaaatgtt gattgataat
    13381 ttggactaaa cgcaaaatga atcatagatt atttcatgac tgttagtaac aatcgtaaaa
    13441 ggaaaagcga gtgttttggt tagctaagtt tagcaattca acgataacca atcagccact
    13501 aacaaatatt tcatgcaata ctcactttga aatacaacaa actttggagg tcataacgat
    13561 gagtaacaaa aacaaatcat atgattatgt catcattgga ggaggcagtg caggttctgt
    13621 actaggtaat cgtctgagtg aagataaaga taaagaagtc ttagtattag aagcgggtcg
    13681 cagtgattat ttttgggatt tatttatcca aatgcctgct gcgttaatgt tcccttcagg
    13741 caataaattt tacgattgga tttattcaac agatgaagaa ccacatatgg gcggtcgtaa
    13801 agtagcacat gcccgaggta aagttttagg tggatcaagt tcgattaatg gcatgattta
    13861 ccaacgtggt aatccaatgg actatgaagg ctgggcagaa ccagaaggta tggaaacttg
    13921 ggattttgcg cactgtttac cgtattttaa aaaattagaa aaaacatacg gtgcagcgcc
    13981 ttatgataaa tttagaggcc atgatggacc aattaagtta aaacgagggc cagcaacgaa
    14041 tcctttattc cagtcattct ttgatgcagg tgttgaagca ggctatcata aaacacctga
    14101 tgtgaatgga tttagacaag aaggttttgg accgttcgat agtcaagtac atcgtggtcg
    14161 ccgaatgtca gcttcaagag catatttaca tccagcgatg aagcgtaaaa acttaaccgt
    14221 tgaaacacgt gcctttgtaa ctgaaattca ttatgaaggt agaagagcaa ctggtgttac
    14281 gtataagaaa aatggcaaac tacataccat cgatgctaat gaagtcattt tgtctggtgg
    14341 ggcattcaat acgccacaat tactacaatt atctggtatc ggtgattcag agttcctaaa
    14401 atcaaaaggc attgagccac gtgttcattt acctggtgtg ggtgaaaact ttgaagatca
    14461 cttagaggta tacattcaac ataaatgtaa ggaacctgta tcattacagc caagcttaga
    14521 tatcaagcgc atgccattta ttggtttaca atggattttc acacgtaaag gtgcagcagc
    14581 atctaaccat tttgaaggtg gcggatttgt acgttcaaat aacgaagttg attatcctaa
    14641 cttaatgttc catttcttac caatagctgt aagatatgat ggtcaaaagg cagcggtcgc
    14701 acatggttat caagttcacg taggaccaat gtattcaaac tctcgtggta gcttgaaaat
    14761 caaatctaaa gatccattcg aaaaaccaag tatccgcttt aattatttat caacagaaga
    14821 agataaaaaa gaatgggtag aagcaattcg tgtagcaaga aatattttat ctcaaaaagc
    14881 aatggatcca ttcaatggtg gagaaatttc accaggacca gaagtgcaaa cagacgaaga
    14941 aattcttgat tgggttcgta gagatggtga aacagcatta cacccatctt gtagtgctaa
    15001 aatgggtcct gcttcagatc caatggcagt agttgacccg ttaacaatga aagtccacgg
    15061 tatggaaaat ttacgtgtag ttgatgcatc agctatgcca cgtacgacaa atggcaatat
    15121 acacgcacct gttttaatgt tagctgaaaa agcagcagat attatacgtg gcagaaagcc
    15181 tttagagcca caatatattg attattataa gcatggtgtt catgatgaaa atgaaggtgc
    15241 aatcgaagta aaaccatacg ctaaataaat gtatatagta gactcttagt ctggcatcta
    15301 ttgtattggt gccaggcttt ttgcgtttta tctaaatgag aagttaatgt gttgttcaaa
    15361 aacgatgtgt gagtatatga actgaaaata gagttaattt tacattgtga tgattaattg
    15421 acctaattgg cagacattaa aagagtgtag aactttaaaa agttcactaa atagacattt
    15481 aattgtgttt tcttgaaagc tttcgtggta ggatttatat aagtaaattg agagaaggaa
    15541 tactatgagc ttttacgaat atatacaaac atttaaagat gataaaacac cattaggcga
    15601 attagcgatt tggattaaag aagatgattc attcccaaaa caagagaagc taacagaaaa
    15661 cattttgtct tattttcatc aaatgtccaa tatagatcat gagtttttag aaattgtaaa
    15721 aagatcactt tctctgtatg atcaattaaa atcgtaaaat cagaaataaa gttagctaaa
    15781 ataaaaagcc ggtaatttca cagatgaagt tgtcggctat tttcatgact agtgttaaat
    15841 aatgatcatg aaattgaaga cacaaaaata gtgtgcgatt cctgtaccca tttgtatgaa
    15901 atattaatac tttttacaaa gtcgtatctt ttaaaaagaa gccaaactat gattgtaatt
    15961 aataaatacg ggaatgaatt agtaatagat ataaagtaat atgaccaaaa atataaatga
    16021 aatgaaggca tttattatga acatttatga tgaatataga agttatgtaa tagaagaact
    16081 ggatgattgt ctcacaatac aaaaaaataa tgacactgca tattacgatg tgctagaagc
    16141 gataaatgat ttatcaaatg attcattatg tgtgttaaat catttatata ttaacgaagg
    16201 acaagaagaa acattcgagc aaaaattttt gcgacgtaat aagcatttaa aaaacgttga
    16261 tgggtttaaa gcattaaggt ttttaagacc tagaacagct ggtagacact atatcattat
    16321 tacgctgtgg gaaaatagga aagcatttta tcattggcaa aattcagctg agtacaagca
    16381 tacacacaag catcgtggca cttcgaaagg tgcagatgta aaaataatta atcgtgaatt
    16441 atcatacaat attcgtattg aattagctga tatggtctag cccgagatat caaccctcaa
    16501 tcatttgaaa gtccttgaat gctacgtcat ggcaaagtgt agtgtcgagg actttggtat
    16561 ttaaccctat ttaattgaaa attctgtaaa gataggcgta tgatatatat aattcattta
    16621 taaaattaca atgttcatca caggaaaagt ggatgtatga aaggaagtta gttatgcaaa
    16681 agtcagctct attcgcgcct gaaaaatata acattatttc ggaaatcgaa aaatatagcc
    16741 acataccaga taaaaaggca attttatatc ataatactga aggcgaagat atatcagtca
    16801 cttaccaaca gctcattgaa cagtctaata aagtaggcaa tgttttagca tcgcacggtt
    16861 tatctaaagg tgataaagta ctcattatga tgccacgatc tattgcgacg tatgaattat
    16921 atattgctgc tttaaaatta ggtgtcgcaa tcattccatg ttctgaaatg ctaagaacta
    16981 aagatttaca ataccgtatc acacatggtg aaattaatgc agttattgca ctagagccat
    17041 tcacagttga atttgaaaaa attaaaggat atgatgcgtt aactaaattt gttattgccg
    17101 gtcaaaaaga tggatggatt tcacttgaag ctgaaaaaga aaacgcaagt aacaaactag
    17161 aaatggcaga tacgacgcga gatgatacag cgattttgtc atatacatct ggtacaactg
    17221 gcaatcctaa agccgttaca cattgtcatg gatggggatt tgcacattta caaatggcac
    17281 caaaacattg gttatgtatt aatgaagatg acttagtttg ggcaacagca gcaccaggtt
    17341 ggcaaaaatg ggtatggagt cctttcttat ctgtattagg ttcaggtgca actgcttttg
    17401 tatttaatgg aagattcagt cctgaaacat acttagaatt gcttcaaaaa tatcaaatta
    17461 atgttttatg ttgtacaccg acagaatatc gtatgatggc taaattacag aacttaaatg
    17521 attatgactt aacgcattta catagtgccg tttctgcagg tgagccacta aatcgagaag
    17581 tagttgaaca attcaagaaa tactttaatt taactgtaag agatggatat ggccagactg
    17641 aaagtacctt gcttataggc tttttaaaag atacaccaca aaggattgga tcaatgggta
    17701 aaggcattcc gggtagttcg gtcactgtag tagatgatga ggggaatagc gtacctgcta
    17761 atacggaagg gaatattgca gtaccacttg atttgccagc acttttcaaa ggatattata
    17821 aggagccaga acgtacggcg aaagcgcaaa caggtaaata ctacatcact ggtgacttag
    17881 ctcatgtaga tgatgatggt tatttctggt ttgaaggacg tcgagatgac attattatta
    17941 gttcaggcta tacgattggg ccttttgaag ttgaagatgc gttaacgaat catccagccg
    18001 ttaaagaatg tgctgtagta gcaaaacctc atgatatacg aggcaatatt gtgaaagcat
    18061 tcgttatttt acaagaccat actgcgggtg atgatacgtt agttaaagaa ctacaacaat
    18121 ttgttaaaaa tgaagttgca ccatataaat atccgagaga gattgaattc gttgacgact
    18181 taccaaaaac aaactcaggt aaaattcgtc gtgttgaact acgagatgct gaaatagaaa
    18241 aatggcaaca acaaaaagat agcaatcaat aaacatagca aagcagtcca atgcgtaagg
    18301 tatgatgata cgcactggac tgcttgttta tttatgatgt tctttgtcgt ctagttataa
    18361 ataataaaat tagtttagaa acaagcgcaa ccagtattaa gaatgagaaa atataagatg
    18421 ttaaacctcg aatatcccaa tttaataaaa atacattact atcaccggca ttaagttgaa
    18481 actcagtgtg tgttaaaata ataattacaa tacctataag tgcaagattc aaaataattt
    18541 tcaatattaa aggtaattga agtggttttc ttggatgcat gtgtattgaa tctccttttt
    18601 atgattgatt ttacacttat attgtatcaa aatagagtat aggtttaaaa tgctataagc
    18661 caaatgtaga ttaaaacaat cgttagctat ttgttgtcaa ataattatga ttgatttgat
    18721 atttatcttc tatgaataaa agatttaata tattgcaaag ttaatgaata attataattt
    18781 acatttacca ctgcttgctt taacatatac atgtaagcac taactatagt attttgttac
    18841 agcgtatcaa ttaaaacagg aagttatcta tggcgttgat attttaaaga aatatgtcag
    18901 ttttatgtaa ctttcatatt gatgatacac atttacaatt tttggagagg tgaaaccaat
    18961 ggctaagtct aatagtaaag acatcgtttt aattggagcc ggtgtactta gcacaacatt
    19021 tggttcaatg ttaaaagaaa ttgagccaga ctggaatatc cacgtttacg aacgcttgga
    19081 tcgtcctgca atcgaaagtt caaacgaaag aaataatgct ggtacgggtc atgcagcatt
    19141 atgtgagttg aactacacag ttttacaacc tgatggttct atcgacatcg aaaaagcgaa
    19201 agtgattaac gaagagtttg agatttcaaa acaattctgg ggtcacttag tgaaaagcgg
    19261 tagcatcgag aatccaagag aatttatcaa tccattacca cacatcagtt atgttagagg
    19321 taaaaacaat gttaaattct taaaagatcg ttacgaagcg atgaaagctt tccctatgtt
    19381 cgataatatc gaatatactg aagacatcga agtaatgaaa aaatggattc cattgatgat
    19441 gaaaggccgt gaagataacc ctggtatcat ggcggcaagt aaaattgacg aaggtacaga
    19501 tgtaaacttc ggtgaattaa cacgtaaaat ggctaaaagc attgaagcac atccaaatgc
    19561 tacagtgcaa tttaaccatg aagttgttga ttttgaacaa ttatcaaatg gtcaatggga
    19621 agttactgtt aaaaatcgcc taactggtga gaaattcaaa caagtaactg actatgtatt
    19681 catcggtgct ggcggtggag caattccatt attacaaaaa acaggtatcc ctgaaagtaa
    19741 gcatttaggt ggattcccta tcagtggtca attcttagct tgtacaaacc cacaagttat
    19801 tgaacaacac gatgccaaag tttatggtaa agagccacct ggtacaccac caatgacagt
    19861 acctcattta gatacgcgtt acattgatgg tcaaagaaca ttattatttg gaccatttgc
    19921 taatgttgga cctaaattct tgaaaaatgg ttctaactta gatttattca aatcagttaa
    19981 aacatacaac attacaactt tattagcagc agcagttaaa aacttacctt taattaaata
    20041 ctcatttgac caagtgatta tgacaaaaga aggttgtatg aaccacttac gtactttcta
    20101 tccagaagca cgtaatgaag attggcaatt atacactgct ggtaaacgtg tacaagttat
    20161 caaagataca cctgaacacg gtaaaggatt catccaattc ggtacagaag tggttaactc
    20221 acaagaccat actgtaatcg cattattagg tgaatcacca ggggcttcaa cttcagtttc
    20281 agttgcgtta gaagtattag aacgtaactt ccctgaatac aaaactgaat gggcacctaa
    20341 aatcaagaaa atgattccat catacggtga atcattaatt gaagacgaaa aattaatgag
    20401 aaaaatccgt aaacaaactt caaaagactt agaattaggt tactacgaaa actaaattct
    20461 aatagctata tttaaaatcg ggcaactgag aaatcttata gatttttcag ctgtccgatt
    20521 ttttacgtaa tctaatacat acggcaaaat caactttaat caaaaaagac tcatacacaa
    20581 tgcccttaaa gcacatgtat gagtccattt tagtagttta tatcaaaaaa tagtttaatg
    20641 tataaattag tttttgttta cagatgcgtc gtagattgat tctacagcat cacctaaagc
    20701 tttatcgaat tcttctttag attgatcagc tcttaaatca ctagctaatg cacgtgagaa
    20761 acttgcgata agttcagcgt tatctttaag taattcattt gctttttctc tgctgtaacc
    20821 acctgataat acaacgacac gaacaacatt aggatgttca gctaactctt tgtataagtt
    20881 tggttcagta ggaattgtta atttcaacat tactaattga tcagcattta agctatctaa
    20941 acctttttta agttcagctt ttaatacttt ttcaatttca gctttgtctt ttgcattaat
    21001 attaacttct ggttcgataa ttggaactaa acctttagca ataatttgtt tagcaacttc
    21061 aaattgttgt tcaacaacgt ctttgatacc ttgctcattt aattctaaaa tgttagaacg
    21121 catttttgta ccaaaaatgt gacgttcgtt tgcacggtct aataaactgt ctaagttgtc
    21181 gattggtttc attaattgaa caccattttg ctcttcagca agacctttgt ctacttttaa
    21241 gaacggaaca acacctttat cagctaagta atctgcagtg tatttgcctt ctacttcgcg
    21301 atccattgtt tgttcgaaaa gaatagcacc caaaatttta tctggtgaga atgaaggtga
    21361 agttaccaca cgtgtacgca tatcgtgaac aagttggaac atttcgtctt cattgctata
    21421 ttgatcttcg tttacaccat attctttaag tgcttttggt gtactaccac cactttggtc
    21481 taatgcggca ataaagcctt ttccattttt cattttttct aattgctctt tattcatact
    21541 ttccactcct taacttttca atacacctcc agtatgataa aaatgagaac atttctcaag
    21601 tcataaacct tgaaaagtgt ataaaatgtg aaaaataatt gtcagtttaa ttaaaaatat
    21661 tattttattc taggtatgac taacgctatt aatgacataa agaaaatatg tgtaataatc
    21721 caaccgatta attctgtcac actaaattga aaaattggac gttgcgcaat aaatactaaa
    21781 aagggataca atgctataaa taagaaaaat aaagggatat aacatagata gtaaagcctt
    21841 ttagaagtat gaaacagcaa cacaaatata aaataaatga cgctaccaat tagtaagtga
    21901 catattattt ctagaatata tggtgtattg tcagaaggaa ttagaaaatc taaattcaat
    21961 aataaagctt ttaaatgtga ttgattgctt attaagtcaa acaacatggt catccatagc
    22021 aacatgaatg atatgaaaat agcagcaatg aaatggaaca atacttttaa acgcattcgt
    22081 atcacctcag agtcaaatga aatatagtaa acaattccct aatcgaacaa aattatgcgc
    22141 ataaacaaag tagattgata taaaattctt aattatcaga atatatttac aaatctgaat
    22201 tttattagta tattggatag tattcataga ggcatgacgg tatttgagca ggattttaaa
    22261 tcggatttta taatcgattt aagagaggcc actttgcttg cacattaata ctgtcaatgg
    22321 gaggggaatg tatatgagta aagcacatca attaattcaa gaggatgaac attattttgc
    22381 gaaatcagga cgtattaaat attatccgtt agtgattgat catggatatg gagcaacatt
    22441 ggttgatatt gaggggaaga catatatcga tttgttatcg agtgcgagtt ctcaaaacgt
    22501 aggtcatgca cctagagaag taacagaagc gataaaggca caagttgata aatttattca
    22561 ttatacacca gcatatatgt atcatgaacc gttggtacgt ttagctaaga agctttgtca
    22621 gattgcacct ggagattttg aaaaaagagt gaccttcgga ttaaccggat cagacgcaaa
    22681 tgatggcatc attaaatttg ccagagcata tacagggcgt ccttatatca ttagtttcac
    22741 taatgcatat catggttcaa cttttggctc attgtctatg tcagctatta gtttaaatat
    22801 gcgcaaacat tatggtccgt tattgaatgg tttttatcat attccgtttc cagataaata
    22861 tcgtggtatg tacgagcagc cacaagctaa ttcagtagaa gaatatttag cacccttaaa
    22921 agaaatgttt gcgaaatatg tacctgctga tgaagtagca tgtattgtta ttgaaacgat
    22981 acaaggcgat ggtggacttt tagaaccagt tccagggtat tttgaaacgt tagaaaagat
    23041 ttgtcgtgaa catggtattt taatcgctgt cgatgatatt caacaaggtt ttgggagaac
    23101 aggtacatgg agttcagtct cgcattttaa ttttacgccc gatttaatca cttttggaaa
    23161 atccttagca ggtggtatgc ctatgtcagc aattgttgga cgcaaagaga ttatgaattg
    23221 tttagaagcg ccagcacatt tatttacaac aggtgctaat ccagttagtt gtgaagctgc
    23281 attagccaca attcaaatga ttgaagatca gtcgcttctt caggctagtg cggaaaaagg
    23341 ggaatatgtt aggaaacgaa tggatcaatg ggtatctaaa tacaatagtg taggcgatgt
    23401 tagaggtaaa ggtctgagca ttggtattga tattgtttcc gacaaaaaac tcaaaacacg
    23461 tgatgccagt gcggcactta aaatttgtaa ttactgcttt gagcatggcg tagttattat
    23521 agctgtagca ggaaatgtgt tgcgattcca accgccattg gtaataacat atgagcaatt
    23581 agacacggcg ttaaacacta tagaagatgc actgactgct ttggaagcag gtaacttaga
    23641 tcaatatgac atatctggac aaggttggta atagcgatta tcttaatata aaataaaaaa
    23701 tcatttccac atctggatgt taatcagatg ggaaatgatt tttttgattt ttattttggt
    23761 gggtggtatt cagctacgtc atttttctta gaatgtctaa gtccataact taaatataga
    23821 atgataccaa caataaacca aattaaagtg tataatttcg cttcgaatcc taatccccag
    23881 aatactagca atactaaaac aaatgtaact gctggtaaca caggatataa aggtaattta
    23941 aatgcaggaa ttggtagatc tttaccttca cgctttctca aacgatacat tgctaatgaa
    24001 acgaacataa atgcaacaag tgtacctgct gaaattaatt gtgctaaaaa tgcgaatggg
    24061 aacatagaac caattaaaac accaataata gtaagtataa ctagtgcgcg attaggtaaa
    24121 tgtttgtcgt ttaaatggct taaccatgaa ggtaataagc cgtcacgtcc aaatgaataa
    24181 agtaaacgtg agcctgctaa catcatacca attaatgctg taaacatacc gattacagag
    24241 atagcttgaa caatagctgc tacaacacca tgaccacttt gacgtaaagc ccaaccaaca
    24301 ggttcagcat tgtttgcgta ttgtgagtaa tggaacatac caactaacac aagtgctaca
    24361 gcaataaata atacgatagc aacacttaaa gaaccaagaa tacctctagg cattgtcttt
    24421 tgagggtcaa gtgcttctgc tgagtttgct gcgatagaat cgaaaccgat atacgctaag
    24481 aaaatcattg aaacaccagc atatatgcct tgccaaccac caaagtcacc tgtagcagtt
    24541 actttgtgtt ctggaataaa tggcacatag ttactaacat ttattgctgt taaacctacg
    24601 atgacaaata aaataatagc taatactttt aaaataacta aaatattttc catacgtgct
    24661 gcttctgaca taccacgtga tagtaataat gcagttaata aaataacgat agcagcaata
    24721 atatcgataa aaccgccatt cgtaccaaat ggatttgata atgctgctgg taattcgatg
    24781 ccaattggtt tcacaagtcc gcgtaaattc gctgagaatc ctgatgcaac aaaggctacg
    24841 gcgataaaat attcagctaa tagagcccaa ccggcaaccc atccaaagaa ttcaccaaat
    24901 aatacattga cccaagaata ggctgaacct gcaaatggca tagcggcagc catttctgca
    24961 taagtaaatg caactaaacc agcaacaata gctgcgagta agaatgataa cgcaactgcc
    25021 ggccctgcat gctgtgcagc aacaatgcca ggtagcgtaa agatagatgt cgatacaatt
    25081 gttccaacac ctaaagctaa gaaatcacgc acccgaagtg tacgctttaa atgaccatct
    25141 ttattttgat agatagccgg atcctctttt cgtgctattt tattgaaaaa acttcccata
    25201 aactttcctc ccaaacattc ataaacaatt ctatacggtg ttttttaata tgttatatca
    25261 tagcacaaat aatcaatatt ttgtctaaaa attctgaaaa atcacaactt tatgttacgt
    25321 attaatgact tgtcttgata acatccgtag attttttaaa tgataaaact gattataaca
    25381 gatattaaat gaataagtac tattttttgc taattttcta acaattttgc acattatatt
    25441 tttaaaatca atttcatgtt tatgtctgat tggttagtgt gtatgaaatg taattctttg
    25501 actataaaaa gatgcatcaa atatgatgag ggtaaggcgg aaaataggag gttatatagt
    25561 aagtcattta agtcgaatag ccatcctttt taaattgaaa aaaatagaaa gctcaatagt
    25621 ttgttaaagc ctttaaatag cgtcattatg caattttaga atgctaaaaa ttgtcacaaa
    25681 tttcaaatta cgtgctaaag cttgagatat caatatttat tggcgataga gtgtaatttg
    25741 actccgctta catctcagtt ttatgtttgt aatttggtag cataatatta taataaaata
    25801 aaattgttaa tctttaattt cagtatagat atttttacgt gtagtcacgt gtaaaataaa
    25861 ttcaattagg ttaggagaca taattatgaa aacatttggt aaaaaggttg tattaatcgg
    25921 agatggatct gtaggatcaa gctatgcctt tgcaatggtt acgcaaggtg ttgctgatga
    25981 atttgtaatt attgacattg caaaagacaa agtaaaagca gatgttcaag atttaaacca
    26041 tggtacagtc cacagtcctt caccagttga tgtgaaagca ggtgaatacg aagactgtaa
    26101 agatgcagat ttagttgtta ttacagctgg tgcacctcaa aagccaggtg aaacacgttt
    26161 acaattagtt gaaaaaaata ctaagattat gaagagcatt gttaagagtg taatggatag
    26221 cggctttgat ggatatttct taatcgcggc aaaccctgta gacattttaa caagatttgt
    26281 aaaagaatat actggattgc cagcagagcg tgttatcggt tcaggtactg tattggacag
    26341 tgcacgttta caatatttaa ttagccaaga acttggtgtt gcaccttcaa gtgttgacgc
    26401 tagtattatt ggtgagcatg gtgatactga acttgcagtt tggtcacaag caaatgtagc
    26461 aggtatttca gtatatgaca cattaaaaga acaaactggt agcgaagcta aagcggaaga
    26521 aatttatgta aatacacgtg acgctgctta tgaaattatc caagctaaag ggtcaacata
    26581 ctatggtatt gcattagcat tgatgcgcat ttcaaaagcc attttaaata atgaaaataa
    26641 tgtcttaaat gtttctatac aattagatgg tcaatatggc ggtcacaaag gcgtttactt
    26701 aggtgtaccg acattggtta accaacatgg cgcagttaaa atttatgaaa tgccattaag
    26761 tgctgaagaa caagcattat tcgacaaatc agttaaaaca ttagaagata catttgattc
    26821 aattaaatat ttattagaag actaagccta ttttaagtat taattagaaa tatattaatg
    26881 gtaagaggat ctattagtgt tgcagtaaca cgtggcactg atagatccat tt
//
LOCUS       DAHHOJ010000026        25997 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.026, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000026 DAHHOJ010000000
VERSION     DAHHOJ010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.026"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(23..238)
                     /locus_tag="NBH32_000945"
     CDS             complement(23..238)
                     /locus_tag="NBH32_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514565.1"
                     /translation="MSINIDPEKFAELVVMSNPSKFEDAEDIAKESLKLYINAYRLAE
                     RYSTIATNCYDTAEVIKELKKTDLQLK"
     gene            412..1209
                     /locus_tag="NBH32_000946"
     CDS             412..1209
                     /locus_tag="NBH32_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HCD1514566.1"
                     /translation="MRIMFIGDIVGKIGRDAIETYIPQLKQKYKPTVTIVNAENAAHG
                     KGLTEKIYKQLLRNGVDFMTMGNHTYGQREIYDFIDEAKRLVRPANFPDEAPGIGMRF
                     IQINDIKLAVINLQGRAFMPDIDDPFKKADQLVKEAQEQTPFIFVDFHAETTSEKYAM
                     GWHLDGRASAVVGTHTHIQTADERILPKGTGYITDVGMTGFYDGILGINKTEVIERFI
                     TSLPQRHVVPNEGRSVLSGVVIDLDKEGKTKHIERILINDDHPFSTF"
     gene            1349..3109
                     /locus_tag="NBH32_000947"
     CDS             1349..3109
                     /locus_tag="NBH32_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HCD1514567.1"
                     /translation="MKPQLSWKVGGQQGEGIESTGEIFATAMNRKGYYLYGYRHFSSR
                     IKGGHTNNKIRVSTTPVHAISDDLDILIAFDQETIDVNHHEMREDSIILADAKAKPVK
                     PEGCHAQLIELPFTATAKELGTALMKNMVAIGATSALMNLNTNTFEELITNMFSKKGD
                     KVVEVNIQALNEGYQLMQSRLPEIYGDFELESTDALPHLYMIGNDAIGLGAIAAGSQF
                     MAAYPITPASEVMEYMIANISKVNGAVIQTEDEIAAVTMAIGANYGGVRAFTASAGPG
                     LSLMMEAIGLSGMTETPLVIINTQRGGPSTGLPTKQEQSDLMQMIYGTHGDIPKIVVA
                     PTDAEDAFYLTMEAFNLAEQYQCPVIVLSDLQLSLGKQTVEKLDYNRIEIKRGEIIQS
                     DIEREEDDKGYFKRYALTSNGVSPRPIPGIKGGIHHITGVEHNEEGKPSESASNRQQQ
                     MEKRMRKIEQLLIELPVEANLQHEDADILYIGFISTKGAIQEGSNRLNQQGIKVNTIQ
                     IRQLHPFPTSVIQDAVNKAKKVVVVEHNYQGQLSSIIKMNVNIHDKIENYTKYDGTPF
                     LPHEIEEKGKIIATEIKEMV"
     gene            3110..3976
                     /locus_tag="NBH32_000948"
     CDS             3110..3976
                     /locus_tag="NBH32_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HCD1514568.1"
                     /translation="MATFKDFRNNVKPNWCPGCGDFSVQAAIQKAAANIGLEPEEVAI
                     ITGIGCSGRLSGYINSYGVHSIHGRALPLAQGVKMANKDLTVIASGGDGDGYAIGMGH
                     TIHALRRNMNMTYIVMDNQIYGLTKGQTSPSSAVGFVTKTTPKGNIEKNVAPLELALS
                     SGATFVAQGFSSDIKGLTKLIEDAINHDGFSFVNVFSPCVTYNKINTYDWFKEHLTSV
                     DDIENYDSTDKQLATKTVIEHESLVTGIVYQDKETPSYESQIKELDDTPLAKRDIKIT
                     EDTFNALTEQFI"
     gene            4070..4363
                     /locus_tag="NBH32_000949"
     CDS             4070..4363
                     /locus_tag="NBH32_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-binding protein"
                     /protein_id="HCD1514569.1"
                     /translation="MKDTLMSIQIIPKTPNNDNVIPYVDEAIKIIDESGLHFRVGPLE
                     TTVQGNMNECLILIQSLNERMVELECPSIISQVKFYHVPDGITIETLTEKYDE"
     gene            4497..6041
                     /gene="miaB"
                     /locus_tag="NBH32_000950"
     CDS             4497..6041
                     /gene="miaB"
                     /locus_tag="NBH32_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCD1514570.1"
                     /translation="MNEEQRKASSVDVLAERDKKAEKDYSKYFEHVYQPPNLKEAKKR
                     GKQEVRYNRDFQIDEKYRGMGNERTFLIKTYGCQMNAHDTEVIAGILEALGYQATTDI
                     NTADVILINTCAIRENAENKVFSEIGNLKHLKKERPDILIGVCGCMSQEESVVNKILK
                     SYQNVDMIFGTHNIHHLPEILEEAYLSKAMVVEVWSKEGDVIENLPKVREGNIKAWVN
                     IMYGCDKFCTYCIVPFTRGKERSRRPEDIIDEVRELAREGYKEITLLGQNVNSYGKDL
                     QDIEYDLGDLLQAISKIAIPRVRFTTSHPWDFTDHMIDVISEGGNIVPHIHLPVQSGN
                     NAVLKIMGRKYTRESYLDLVKRIKDRIPNVALTTDIIVGYPNESEEQFEETLTLYDEV
                     GFEHAYTYLYSQRDGTPAAKMKDNVPLDVKKERLQRLNKKVGHYSQIAMSKYEGQTVT
                     VLCEGSSKKDDQVLAGYTDKNKLVNFKAPKEMIGKLVEVRIDEAKQYSLNGSFVKEVE
                     PEMVIQ"
     gene            6042..6407
                     /locus_tag="NBH32_000951"
     CDS             6042..6407
                     /locus_tag="NBH32_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RicAFT regulatory complex protein RicA family
                     protein"
                     /protein_id="HCD1514571.1"
                     /translation="MYNKDDVLKQADNIANKIKNLDTIKTYQQIEAQIHQNQTIKTKM
                     DMLKKHQKQAVNFQNYGKQNALEQSEHTIQSIEAEINTLPIVEQFQTSQYEANQLLKM
                     FVSTMETRLNDHNKAKHSD"
     gene            6434..6925
                     /gene="thiW"
                     /locus_tag="NBH32_000952"
     CDS             6434..6925
                     /gene="thiW"
                     /locus_tag="NBH32_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy coupling factor transporter S component
                     ThiW"
                     /protein_id="HCD1514572.1"
                     /translation="MKSRKLAITALLIAINVVLSSIIIIPLGPVKAAPVQHFVNVLSA
                     VIVGPWYGLAQALISSILRVLFGTGTAFAFPGSMIGVLLASMFYIYRKHIFMAAVGEV
                     LGTGVIGSLICIPLAYFLGLQDFFIKPLMITFIVSSAIGSIISYFLLITLKKRGILQR
                     FIK"
     gene            7228..9846
                     /gene="mutS"
                     /locus_tag="NBH32_000953"
     CDS             7228..9846
                     /gene="mutS"
                     /locus_tag="NBH32_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCD1514573.1"
                     /translation="MSNVTPMMQQYLKIKSEYQDCLLFFRLGDFYEMFYEDAKEASRV
                     LEITLTKRDAKKENPIPMCGVPYHSADSYIDTLVNNGYKVAICEQMEDPKQTKGMVRR
                     EVVRIVTPGTVMEQGGVDDKQNNYILSFVMNQPEIALSYCDVSTGELKVTHFNDEATL
                     LNEITTINPNEVVINDNISDNLKRQINMVTETITVRETLSSEIYSVNQTEHKLMYQAT
                     QLLLDYIHHTQKRDLSHIEDVVQYAAIDYMKMDFYAKRNLELTESIRLKSKKGTLLWL
                     MDETKTPMGARRLKQWIDRPLISKEQIEARLDIVDEFSAHFIERDTLRTYLNQVYDIE
                     RLVGRVSYGNVNARDLIQLKHSISEIPNIKALLNSMNQNTLVQVNQLEPLDDLLDILE
                     QSLVEEPPISVKDGGLFKVGFNTQLDEYLEASKNGKTWLAELQAKERQRTGIKSLKIS
                     FNKVFGYFIEITRANLQNFEPSEFGYMRKQTLSNAERFITDELKEKEDIILGAEDKAI
                     ELEYQLFVQLREEVKKYTERLQQQAKIISELDCLQSFAEIAQKYNYTRPSFSENKTLE
                     LVESRHPVVERVMDYNDYVPNNCRLDNETFIYLITGPNMSGKSTYMRQVAIISIMAQM
                     GAYVPCKEAVLPIFDQIFTRIGAADDLVSGKSTFMVEMLEAQKALTYATEDSLIIFDE
                     IGRGTSTYDGLALAQAMIEYVAETSHAKTLFSTHYHELTTLDQALPSLKNVHVAANEY
                     KGELIFLHKVKDGAVDDSYGIQVAKLADLPEKVISRAQVILSEFEASAGKKSSISNLK
                     MVENEPEINQENLNLSVEETTDTLSQKDFEQASFDLFENDQESEIELQIKNLNLSNMT
                     PIEALVKLSELQNQLK"
     gene            9859..11868
                     /gene="mutL"
                     /locus_tag="NBH32_000954"
     CDS             9859..11868
                     /gene="mutL"
                     /locus_tag="NBH32_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCD1514574.1"
                     /translation="MGKIKELQTSLANKIAAGEVVERPSSVVKELLENAIDAGATEIS
                     IEVEESGVQSIRVVDNGSGIEAEDLGLVFHRHATSKLDQDEDLFHIRTLGFRGEALAS
                     ISSVAKVTLKTCTDNANGNEIYVENGEILNHKPAKAKKGTDILVESLFYNTPARLKYI
                     KSLYTELGKITDIVNRMAMSHPDIRIALISDGKTMLSTNGSGRTNEVMAEIYGMKVAR
                     DLVHISGDTSDYHIEGFVAKPEHSRSNKHYISIFINGRYIKNFMLNKAILEGYHTLLT
                     IGRFPICYINIEMDPILVDVNVHPTKLEVRLSKEEQLYQLIVSKIQEAFKDRILIPKN
                     NLDYVPKKNKVLHSFEQQKIEFEQRQNTENNQEKTFSSEESNSKPFMEENQNDEIVIK
                     EDSYNPFVTKTSESLIADDESSGYNNTREKDEDYFKKQQEILQEMDQTFDSNDDTTVQ
                     NYENKASDDYYDVNDIKGTKSKDPKRRIPYMEIVGQVHGTYIIAQNEFGMYMIDQHAA
                     QERIKYEYFRDKIGEVTNEVQDLLIPLTFHFSKDEQLVIDQYKNELQQVGIMLEHFGG
                     HDYIVSSYPVWFPKDEVEEIIKDMIELILEEKKVDIKKLREDVAIMMSCKKSIKANHY
                     LQKHEMSDLIDQLREAEDPFTCPHGRPIIINFSKYELEKLFKRVM"
     gene            11874..12416
                     /locus_tag="NBH32_000955"
     CDS             11874..12416
                     /locus_tag="NBH32_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
                     /protein_id="HCD1514575.1"
                     /translation="MNQVNNNILPAIRNIKDLEKLIKTDYKMCVLLDMHIGHIKSIME
                     LLKQNHIECFIHIDLIKGLSHDEFASEFIIQQYKPKGIVSTKSKVIKKAKSLNTLTIF
                     RVFIIDSQALKRSIDLIKKVEPDFVEVLPGVASKAIHHIQKETNTQVIAGGLINTIDE
                     VNEAVKNGAKYVTTSYDKLW"
     gene            12881..13699
                     /locus_tag="NBH32_000956"
     CDS             12881..13699
                     /locus_tag="NBH32_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HCD1514576.1"
                     /translation="MNVYLAEFLGTAILILFGGGVCANVNLKRSAANGADWIVITAGW
                     GLAVTMGVFAVGQFSGAHLNPAVSLALALDGSFDWSLVPGYIVAQMLGAIVGATIVWL
                     MYLPHWKATEEAGAKLGVFSTAPAIKNYFANFLSEIIGTMALTLGILFIGVNKIADGL
                     NPLIVGALIVAIGLSLGGATGYAINPARDLGPRIAHAILPIAGKGGSNWSYAIVPILG
                     PIAGGLLGAVVYAVFYKHTFNIGCAIAIVVVIITLILGYILNKSSKKGDIESIY"
     gene            13828..15324
                     /gene="glpK"
                     /locus_tag="NBH32_000957"
     CDS             13828..15324
                     /gene="glpK"
                     /locus_tag="NBH32_000957"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCD1514577.1"
                     /translation="MEKYILSIDQGTTSSRAILFNQKGEIAGVAQREFKQYFPQSGWV
                     EHDANEIWTSVLAVMTEVINENDVRADQIAGIGITNQRETTVVWDKHTGRPIYHAIVW
                     QSRQTQSICSELKQQGYEQTFRDKTGLLLDPYFAGTKVKWILDNVEGAREKAENGDLL
                     FGTIDTWLVWKLSGKAAHITDYSNASRTLMFNIHDLEWDDELLELLTVPKNMLPEVKA
                     SSEVYGKTIDYHFYGQEVPIAGVAGDQQAALFGQACFERGDVKNTYGTGGFMLMNTGD
                     KAVKSESGLLTTIAYGIDGKVNYALEGSIFVSGSAIQWLRDGLRMINSAPQSESYATR
                     VDSTEGVYVVPAFVGLGTPYWDSEARGAIFGLTRGTEKEHFIRATLESLCYQTRDVME
                     AMSKDSGIDVQSLRVDGGAVKNNFIMQFQADIVNTSVERPEIQETTALGAAFLAGLAV
                     GFWESKDDIAKNWKLEEKFDPKMDEGEREKLYRGWKKAVEATQVFKTE"
     gene            15482..17155
                     /locus_tag="NBH32_000958"
     CDS             15482..17155
                     /locus_tag="NBH32_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HCD1514578.1"
                     /translation="MALSTFKREHIKKNLRNDEYDLVIIGGGITGAGIALDASERGMK
                     VALVEMQDFAQGTSSRSTKLVHGGLRYLKQFQIGVVAETGKERAIVYENGPHVTTPEW
                     MLLPMHKGGTFGKFSTSIGLGMYDRLAGVKKSERKKMLSKKETLAKEPLVKKEGLKGG
                     GYYVEYRTDDARLTIEVMKRAAEKGAEIINYTKSEHFTYDKNQQVNGVKVIDKLTNEN
                     YTIKAKKVVNAAGPWVDDVRSGDYARNNKKLRLTKGVHVVIDQSKFPLGQAVYFDTEK
                     DGRMIFAIPREGKAYVGTTDTFYDNIKSSPLTTQEDRDYLIDAINYMFPSVNVTDEDI
                     ESTWAGIRPLIYEEGKDPSEISRKDEIWEGKSGLLTIAGGKLTGYRHMAQDIVDLVSK
                     RLKKDYGLTFSPCNTKGLAISGGDVGGSKNFDAFVEQKVDVAKGFGIDEDVARRLASK
                     YGSNVDELFNIAQTSQYHDSKLPLEIYVELVYSIQQEMVYKPNDFLVRRSGKMYFNIK
                     DVLDYKDSIIDIMADMLDYSPAQIEAYTEEVEQAIKEAQHGNNQPAVKE"
     gene            17305..18219
                     /locus_tag="NBH32_000959"
     CDS             17305..18219
                     /locus_tag="NBH32_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1514579.1"
                     /translation="MTEKQFKLTVQDNTNIEVKVNFTDVDSKGIIHIFHGMAEHMERY
                     DKLAHALSKDGFDVICHNHRGHGINIDESTRGHYDDMKRVIGDAFEVAQTVRGNVDKP
                     YIIIGHSMGSVIARLFVETYPQYVDGLILSGTGMYPLWKGLPTVKVLQLITKIYGAEK
                     RVEWVNQLVSNSFNKKIRPLRTQSDWISSNPTEVDNFIKDPYSGFNVSNQLLYQTAYY
                     MLHTSQLKNIKMLNHAMPILLVSGCDDPLGDYGKGILKLANIYRKAGIKNVKVNLYHH
                     KRHEVLFEKDHDKIWEDLFKWLNQFYKK"
     gene            18237..19172
                     /gene="miaA"
                     /locus_tag="NBH32_000960"
     CDS             18237..19172
                     /gene="miaA"
                     /locus_tag="NBH32_000960"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCD1514580.1"
                     /translation="MNKNKPFIVVIVGPTASGKTELSIELAKRINGEIISGDSMQVYK
                     HMNIGTAKVTPEEMDGIPHHLIDILNPDDTFSAYEFKRLAEDLITDITNRGKVPIIAG
                     GTGLYIQSLIYNYELEDETVTPAQLSIVKQKLSALEHLDNQQLHDYLDQFDAVSAENI
                     HPNNRQRVLRAIEYYLKTKKLLSNRKKVQQFTENYDTLLLGIEMSRKTLYSRINKRVD
                     IMLDHGLFREVQQLVEQGYESCQSMQAIGYKELISVINGQMIYEDAVNDLKQHSRQYA
                     KRQMTWFKNKMSVHWLDKENMSLQMMLDEITTQIK"
     gene            19187..19420
                     /gene="hfq"
                     /locus_tag="NBH32_000961"
     CDS             19187..19420
                     /gene="hfq"
                     /locus_tag="NBH32_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCD1514581.1"
                     /translation="MIANENIQDKALENFKANQTEVTVFFLNGFQMKGVIEEYDKYVV
                     SLNSQGKQHLIYKHAISTYTVETEGQASTESEE"
     gene            complement(19642..20118)
                     /locus_tag="NBH32_000962"
     CDS             complement(19642..20118)
                     /locus_tag="NBH32_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HCD1514582.1"
                     /translation="METIYDFVVETNKGVTYKLDAYKGDVMLIVNTASECGFTSQFEG
                     LQSLYEKYKDQGFVILGFPCNQFGGQEPGSGEEAAQNCKLNYGVTFPMHQKIDVKGEH
                     QLPLFRYLTAAQHGFFNEKIKWNFTKFLVDREGNVVKRFAPQKKPVQIEREIEKLL"
     gene            20230..21468
                     /gene="hflX"
                     /locus_tag="NBH32_000963"
     CDS             20230..21468
                     /gene="hflX"
                     /locus_tag="NBH32_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCD1514583.1"
                     /translation="MAQQQIHDIKIKLEKAVLVGVHAQDDKQFNFESTMEELSSLSET
                     CQLEVLGQITQNRDRVDRKYYVGKGKIEEIQAFIEFKDIDVVITNDELTTAQSKSLNE
                     ALGVKIIDRTQLILEIFALRARSKEGKLQVELAQLDYLLPRLQGHGKSLSRLGGGIGT
                     RGPGETKLEMDRRHIRTRMNEIKHQLRTVEEHRERYRNKRNQNQVFQVALVGYTNAGK
                     SSWFNVLANEETYEKDQLFATLDPKTRQIQINDGFNLIISDTVGFIQKLPTTLIAAFK
                     STLEEAKGADLLVHVVDSSHPEYRTQYDTVNDLIKQLDMSHISQIVIFNKKDLCDHAS
                     NRPASDLPNVFVSSKNDGDKLLVKTLFIDEIKRRLTYYDEAIATNNADRLYFLKQHTL
                     VTELKYDEIENVYRIKGFKK"
     gene            21487..22725
                     /locus_tag="NBH32_000964"
     CDS             21487..22725
                     /locus_tag="NBH32_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1514584.1"
                     /translation="MKDISKIVADVESTLAPYFKEIEETAYINQEKVLNAFHHVKATE
                     SDLQGSTGYGYDDFGRDHLEEIYAQAFKAEDAIVRPQIISGTHAITIALQSLLKHGDE
                     LIYITGSPYDTLLEVIGVNGNGIESLMEYGVSYKDIALKEGKIDIESVLDGVSERTKV
                     IAIQRSKGYDQRPSIPLDEIEQVIIRLKEVHPNILIFVDNCYGEFVERREPIECGADL
                     IAGSLIKNPGGGLAKIGGYIAGRKDLIERCGYRLTAPGIGKEAGASLNALLEMYQGFF
                     LAPHVVSQSLKGALFTSLFLEKMNMNTTPKYYEKRTDLIQTVKFKTKEQMISFCQSIQ
                     HASPINAHFSPEPSYMPGYEDDVIMAAGTFIQGSSIELSADGPIRPPYEAYVQGGLTY
                     EHVKIAVTRAVNQLKEQGLI"
     gene            22969..23337
                     /locus_tag="NBH32_000965"
     CDS             22969..23337
                     /locus_tag="NBH32_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1514585.1"
                     /translation="MISNDAIRRNMAVFSMSVVSKLTDLTPRQIRYYETHELIKPERT
                     EGQKRLFSLNDLERLLEIKSLLEKGFNIKGIKQIIYDSQEHLTTDEQEIRKKMIVDAT
                     QKPIGETLPINRGDLSRFIK"
     gene            23356..24696
                     /gene="glnA"
                     /locus_tag="NBH32_000966"
     CDS             23356..24696
                     /gene="glnA"
                     /locus_tag="NBH32_000966"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HCD1514586.1"
                     /translation="MPKRTFTKDDIRKFAEEENVRYLRLQFTDILGTIKNVEVPVSQL
                     EKVLDNEMMFDGSSIEGFVRIEESDMYLHPDLDTWVIFPWTAGQGKVARLICDVYKTD
                     GTPFEGDPRANLKRVLKEMEDLGFTDFNLGPEPEFFLFKLDEKGEPTLELNDDGGYFD
                     LAPTDLGENCRRDIVLELEDMGFDIEASHHEVAPGQHEIDFKYADAVTACDNIQTFKL
                     VVKTIARKHNLHATFMPKPLFGVNGSGMHFNVSLFKDKENAFFDPNTEMGLTETAYQF
                     TAGVLKNARGFTAVCNPLVNSYKRLVPGYEAPCYIAWSGKNRSPLIRVPSSRGLSTRI
                     EVRSVDPAANPYMALAAILEAGLDGIKNKLKVPEPVNQNIYEMNREEREAVGIQDLPS
                     TLYTALKAMRENEVIKKALGNHIYNQFINSKSIEWDYYRTQVSEWERDQYMKQY"
     gene            25189..25386
                     /locus_tag="NBH32_000967"
     CDS             25189..25386
                     /locus_tag="NBH32_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514587.1"
                     /translation="MNCYDEIFNTIKELIENKEISSYQINKDTGISYGNINAMRRRER
                     RIENLSLKNAKILYEYAKKVL"
BASE COUNT         9635 a         3524 c         4913 g         7925 t
ORIGIN      
        1 taggatttat ttctaagttg agttatttta attgtaaatc tgttttcttt aattctttta
       61 taacttctgc agtatcataa caatttgttg caattgttga atatctctct gctaaacgat
      121 atgcattaat gtaaagcttt aaactttctt tagctatatc ctctgcatct tcgaattttg
      181 atgggttaga cataaccact aattctgcaa atttttctgg atcaatatta atagacatgt
      241 atttatttac aactcctatt tattttgatg tcttaatact aacatattga agttttcaga
      301 caaagtaatg tctctttata attgaagaaa aataattcaa agaatttaat atttacttta
      361 aatattagaa cgtatgaatt aaactagtat ttatgagagg atgaacaaaa catgagaata
      421 atgtttatag gggatatcgt aggtaaaatt ggacgagacg caattgaaac gtacatacct
      481 caactgaagc aaaagtataa accaacagtt acaattgtaa atgctgaaaa tgcagcacat
      541 ggtaaaggtt tgactgaaaa aatatataaa caattactaa gaaatggtgt agatttcatg
      601 actatgggta atcacacata tggtcaacgt gaaatttatg attttataga tgaagcaaaa
      661 cgactagtaa gaccagcgaa ttttccggat gaagcgccgg gaattggtat gagatttata
      721 caaattaatg atattaaact tgcagttatt aatctgcaag gaagagcgtt tatgccagat
      781 attgatgatc cttttaaaaa ggcagatcaa ttagtcaagg aagcacaaga acaaactccg
      841 tttatatttg ttgattttca tgcagaaaca acttctgaaa agtatgcaat gggatggcat
      901 ttagatggta gagctagcgc tgttgttgga acgcatacac acattcaaac agcagatgaa
      961 cgtattttac caaaggggac agggtatata acggatgttg gtatgacagg tttttatgac
     1021 ggcattttag gaataaataa aacagaggta attgagcgtt ttatcactag tttgccacaa
     1081 agacatgttg ttccaaatga aggtagaagt gtattatctg gtgttgttat tgatttagac
     1141 aaagaaggta aaacgaagca catcgaacgt atattgataa atgatgacca tccattttca
     1201 acattttaaa attacgtaag taaacattcg aattggaccc tatcgtccat tagtatgaat
     1261 ttaatatagt accactgttt acatagtaaa tcggtggttc tttttgttat catttaatat
     1321 gaaatatatc cataggaggc atataactat gaaaccacaa ttatcgtgga aagttggcgg
     1381 tcaacaaggc gaaggtattg aatcaactgg ggaaatcttc gctacggcta tgaatagaaa
     1441 aggatattat ttatatggat atagacattt ttcaagtcgt atcaaaggtg gacatacgaa
     1501 taataaaatt agagtttcta cgacgcctgt tcatgcaatt agtgatgatt tagatatttt
     1561 gattgcattt gaccaagaaa caattgatgt taaccatcat gaaatgagag aagacagtat
     1621 tattttagct gatgccaagg ctaaacctgt gaaaccagaa ggatgtcatg cacagcttat
     1681 tgaattacct tttacagcaa ccgctaaaga attaggtaca gcattaatga aaaacatggt
     1741 tgcaataggt gctactagcg cattgatgaa tttgaataca aatacatttg aagaacttat
     1801 tactaatatg ttttctaaaa aaggtgacaa ggtagttgaa gtcaatatcc aagcattaaa
     1861 cgaaggttat caattaatgc aatctcgctt acctgaaatc tacggggact ttgaattaga
     1921 gtcaacagat gcactaccac atctatatat gattggtaac gatgccattg gattaggtgc
     1981 aattgctgca ggttcgcaat ttatggcggc atatcctatt acacctgcgt ctgaagttat
     2041 ggaatatatg attgccaata tatctaaagt aaacggagcg gttattcaaa cagaagatga
     2101 aattgctgct gtaactatgg ctattggtgc aaattatggt ggagtcagag cgtttacggc
     2161 tagtgctggt ccaggtttat ctttaatgat ggaagcaatt ggattatctg gtatgactga
     2221 aacgccatta gtcattatta atacccaacg aggtggacct tctactggat tacctacgaa
     2281 acaagaacag tcagatttaa tgcaaatgat ttatggtaca catggtgata ttccaaaaat
     2341 tgttgtagca ccaactgatg cagaagatgc attttattta actatggaag cctttaattt
     2401 agcagaacaa tatcaatgcc ctgttatagt tctaagtgat ttgcaattat ctttaggtaa
     2461 acaaactgtt gaaaaattag attataatcg cattgaaatt aaacgtggtg aaatcattca
     2521 atctgatatt gaacgtgaag aagatgataa aggttatttc aagcgttatg cgttaacatc
     2581 caatggtgtt tctcctagac ctatccccgg tattaaagga ggtattcatc atataactgg
     2641 tgtggaacac aatgaagaag gtaaacctag tgaatctgca tcaaatagac aacaacaaat
     2701 ggaaaaacga atgcgtaaaa ttgagcagtt actaattgaa ttgccagtag aagctaactt
     2761 acaacatgaa gatgcagata ttctttatat cggttttatt tctacaaaag gtgcaattca
     2821 agaaggtagt aaccgtttga atcaacaagg cataaaagtt aacactatac aaattagaca
     2881 attgcatcca ttcccaacaa gcgttattca agatgcagtt aataaagcga agaaagtcgt
     2941 tgtagtggag cacaattatc aaggacaatt gtctagtatt ataaaaatga atgtcaatat
     3001 tcatgataag attgaaaatt atacaaagta tgatgggaca cctttcctac cacatgaaat
     3061 cgaagaaaaa ggcaaaataa ttgctactga aataaaggag atggtataga tggcgacatt
     3121 taaagatttt agaaataatg ttaagcctaa ctggtgccct ggatgtggcg atttctcagt
     3181 acaagctgca attcaaaaag cagccgcaaa tatagggtta gaacctgaag aagtagctat
     3241 catcaccggt ataggatgtt ctggccgtct ttcaggatat attaattctt atggcgttca
     3301 ttctattcac ggacgtgcat tacctttagc tcaaggtgta aaaatggcga ataaagattt
     3361 aactgttatt gcatctggag gagatggtga tggttatgct ataggtatgg ggcatacaat
     3421 ccatgcttta agaagaaata tgaacatgac gtatatagtc atggataatc aaatttatgg
     3481 tttgacaaag ggacaaacat cgccgtcatc agcagtagga tttgttacta aaacaacgcc
     3541 aaaaggtaat atagaaaaaa atgttgcgcc tttagaatta gcattatcat ctggtgccac
     3601 atttgtagcc caaggttttt caagcgatat taaaggatta acaaaactaa ttgaagatgc
     3661 aattaatcat gatggatttt cattcgttaa tgtcttttca ccttgtgtga cttataataa
     3721 aattaacaca tacgattggt ttaaagaaca tttaacaagt gttgatgaca ttgaaaatta
     3781 tgattctaca gataaacaat tagcgactaa aactgttatt gaacatgaat ctttagtaac
     3841 tggtattgtt tatcaagata aagaaacacc atcatatgaa tctcaaatta aagagttaga
     3901 tgatacacca cttgctaaaa gagatatcaa aattactgaa gacaccttca atgcattaac
     3961 tgaacaattt atttaataat taaaactgag tcgtataaat gtatttataa cagatccatt
     4021 tatgctactc agttttttac tattacaaaa aataaaggag tttttaaaaa tgaaagacac
     4081 attaatgagt atacaaataa ttcctaaaac accaaacaat gacaatgtta ttccttacgt
     4141 agacgaggcg attaaaataa ttgacgaatc tggtttgcat tttagagtag gtccgttaga
     4201 aacgacagta caaggaaata tgaatgaatg tttaatttta atacaatcat taaatgaacg
     4261 aatggtggaa cttgaatgtc caagtattat tagccaagtt aagttttatc atgtgccaga
     4321 tggcatcact attgaaactt taactgaaaa atatgatgaa taacattaaa agtgaagtaa
     4381 actggatttg aattggcttg ttagagatga cgtataactt taactgtttt tgcactttat
     4441 agttaaattt aatataatta ttaaatgata cgggcaaata gaaaggattt tgtaaagtga
     4501 acgaagaaca aagaaaagca agttctgtag atgttttagc tgagagagat aagaaagcag
     4561 aaaaagatta tagtaaatat tttgaacatg tttatcagcc gcctaattta aaagaagcga
     4621 aaaaaagagg taaacaagaa gttcgttata atagagattt ccaaattgat gaaaaatatc
     4681 gcggtatggg gaacgagcgt acatttttaa ttaaaacata tggatgtcaa atgaatgcac
     4741 atgacactga ggtcattgct ggtatacttg aagcattagg ctatcaagca acgactgata
     4801 ttaacactgc agatgttatt ttaattaata catgtgcgat tagagaaaat gccgagaaca
     4861 aagtgtttag tgaaataggt aatttgaagc atttgaaaaa agaacgacct gatattttaa
     4921 tcggtgtttg tggttgtatg tcacaagaag agtcagtagt gaataaaatt ttaaaatcgt
     4981 atcaaaatgt agatatgata tttggtacac ataatattca tcatttacca gaaattttag
     5041 aagaagcata cttatctaaa gcaatggttg ttgaagtatg gtctaaagaa ggagacgtta
     5101 ttgaaaatct tccaaaagtc cgtgaaggca acattaaagc atgggtcaat attatgtatg
     5161 gttgtgataa gttttgtaca tattgtattg ttccatttac aagaggtaaa gaacgaagcc
     5221 gcagacctga agacattata gatgaagtac gtgaacttgc tcgtgaaggt tacaaagaaa
     5281 taacgctttt aggtcaaaat gtaaattctt atggtaaaga tttacaggat atagaatatg
     5341 acttaggaga tcttttacaa gcaatttcta aaatagcgat tccaagagtt cgtttcacaa
     5401 caagtcatcc ttgggacttt acagatcaca tgattgatgt tatttcagag ggtggtaata
     5461 tcgttccgca tatccacttg ccagttcaat ctggaaataa tgcagtatta aaaataatgg
     5521 gtagaaaata tacacgagaa agttatttgg atttagtaaa acgaatcaaa gatagaattc
     5581 ctaatgtagc attaactaca gatattattg tagggtatcc aaatgaatca gaggaacaat
     5641 ttgaagaaac tttaactctg tatgatgaag ttggttttga acatgcatat acgtatttgt
     5701 attcacaacg tgatggtacg cctgctgcta aaatgaaaga taatgtacct ttagatgtca
     5761 aaaaggaacg attgcaacgt ttgaataaaa aagttggtca ttattcacaa atagctatga
     5821 gtaagtacga aggacaaact gtaacagtac tttgtgaagg tagtagtaaa aaagatgatc
     5881 aggttcttgc tggctacact gataaaaata agctagttaa tttcaaagcg cctaaagaaa
     5941 tgattggtaa actagtggaa gtacgaatag atgaagctaa acagtattca ttaaatggca
     6001 gttttgtaaa ggaagtagag ccggaaatgg tgattcaata aatgtataat aaagatgacg
     6061 tgttgaaaca agcggataat attgcaaata aaattaaaaa tttggatact atcaaaacat
     6121 atcaacaaat tgaagcacag attcatcaga accaaacgat aaagactaaa atggatatgt
     6181 taaaaaagca tcaaaaacaa gcagtaaact ttcaaaatta cgggaaacaa aatgcgctag
     6241 aacagtcgga acataccatt caaagtatag aagcagaaat aaatacattg cccatagttg
     6301 aacagtttca aacttcacaa tatgaagcga atcaattatt gaaaatgttt gtatcaacaa
     6361 tggaaacacg tttaaatgac cataataaag ccaagcatag tgattaatta caaacaaaaa
     6421 ggagaacaat catatgaaat caagaaaact ggctataact gcacttttaa ttgcaataaa
     6481 tgttgtatta agcagtatta tcatcattcc tctaggacca gttaaggcag caccagtaca
     6541 gcattttgta aatgtattaa gtgcggtcat agtaggtcct tggtatggat tagctcaagc
     6601 gcttatatca tcaattttaa gagttctttt tggtactggt acagcttttg catttccagg
     6661 tagtatgatt ggagttttat tggctagtat gttttacata tatcgtaaac atatattcat
     6721 ggccgcggtc ggtgaagtac ttggaactgg tgtcatcgga agtttaattt gtataccatt
     6781 agcatatttc cttgggcttc aagacttctt cattaaaccg ttaatgatta cgttcatagt
     6841 ctcaagtgct atcggatcta ttataagtta tttcttatta attactctaa aaaaacgtgg
     6901 tattcttcaa aggtttataa aataatttta tgatatatga tttcaaacag agtcattgtt
     6961 cattttaata ttgaacgccc ttacttaatt aaatgtcttg aatgttagaa ttttaattta
     7021 attctaaaca tgctattgac acattttatt aggtttgagg cgttttctta tacatttaag
     7081 atgcttcgtg attatatggt atgaataggt ttgtaaaagt aagtatgtta aaatgtataa
     7141 gttaaataat taataattag aaagcttaca aacgaatata ttgtaaagac aaattttaaa
     7201 aagagacaag ggttgtgaag aaataatatg tctaatgtta caccaatgat gcagcaatat
     7261 ttaaaaataa aatcagaata ccaagattgc ttattatttt ttagactagg tgatttctat
     7321 gaaatgtttt atgaagatgc caaggaggca tcacgtgtac ttgaaattac tttaactaaa
     7381 agagatgcta aaaaagaaaa tccaattccg atgtgtggtg ttccgtatca ttctgcagat
     7441 agttatatag atacacttgt taataatgga tataaagtag ctatttgtga acagatggaa
     7501 gatccgaaac aaacgaaagg tatggttaga cgtgaggtag taagaattgt gactccagga
     7561 actgtgatgg agcaaggtgg tgtagatgat aaacaaaata actatatttt aagttttgtt
     7621 atgaatcaac ctgaaattgc gcttagttac tgtgatgttt ctactggcga attaaaggtt
     7681 acacatttta atgatgaagc gactttatta aatgaaatta cgacgataaa ccctaacgaa
     7741 gttgttatca atgacaatat ttccgataat ttaaaaagac aaattaatat ggtgacagaa
     7801 acaataacag tcagggaaac gttatcatca gaaatctata gtgtgaatca aactgaacat
     7861 aaattaatgt atcaagcgac acaattattg ctagattata ttcatcatac acaaaaacgt
     7921 gatttatcgc atatcgagga tgttgttcaa tatgcagcta tagattatat gaaaatggat
     7981 ttttatgcta agagaaacct tgagttaacg gaaagcattc gattaaaatc aaaaaaagga
     8041 acgctacttt ggctaatgga cgaaacgaaa acaccaatgg gagcacgccg cttaaaacaa
     8101 tggatagata gaccactaat aagtaaagaa caaattgaag cacgattaga tatcgttgat
     8161 gaatttagtg ctcatttcat agaaagagac accttaagaa catatcttaa tcaagtgtat
     8221 gatattgaac gtcttgttgg gcgtgttagt tatggaaatg ttaatgcgag agatttaatt
     8281 caacttaaac attccatttc tgaaataccg aatattaaag cattactaaa ttctatgaat
     8341 cagaatactc ttgtacaagt taatcaacta gaaccccttg atgatttact tgatatatta
     8401 gaacagagtt tagtagaaga accaccaatt tcagttaaag atggcggact attcaaagtt
     8461 ggttttaata cgcaattaga tgaatatctt gaagcttcaa aaaacggaaa aacatggtta
     8521 gcagaattac aagccaaaga aagacaacgt acaggaataa aatcattgaa aataagcttt
     8581 aataaagtgt ttggttattt tatagaaata acacgtgcca acttgcaaaa ttttgaacca
     8641 agtgaatttg gttatatgag gaagcaaacg ttatcgaatg cggaacgttt tataactgat
     8701 gaacttaaag aaaaagaaga tatcatttta ggtgcggaag acaaagccat cgaattagaa
     8761 tatcaattat ttgttcagct acgtgaagaa gttaaaaaat atactgaacg tttacaacaa
     8821 caagctaaaa ttatttcaga gctagattgt ttacagagct ttgcagaaat tgctcaaaaa
     8881 tataattaca ctaggccttc atttagtgaa aataaaacat tagaattagt ggaatctagg
     8941 cacccagtag tggaaagagt aatggattat aatgactatg tgcctaataa ttgtcgatta
     9001 gataatgaaa catttatata tttaattaca ggtccgaata tgtctggtaa atcgacatat
     9061 atgagacaag ttgccataat tagtataatg gcccaaatgg gagcttatgt cccttgtaaa
     9121 gaggcagtgt tacctatatt tgatcaaata ttcactagaa taggtgcggc agatgatttg
     9181 gtttcaggta agagtacgtt tatggtagaa atgctagaag cacaaaaggc attaacttat
     9241 gcaacagagg atagtttgat tattttcgat gaaattggac gtggtacttc aacgtatgac
     9301 ggtttagctt tagcgcaggc aatgatagag tatgtagctg aaacatcaca tgctaaaacg
     9361 ttattttcaa cacattatca tgaattgaca acattagatc aagcattacc aagtctaaaa
     9421 aatgttcacg tcgctgctaa tgaatataaa ggtgaactta tattcttgca taaagtcaaa
     9481 gatggtgcag ttgacgatag ttatggtatt caagttgcga aattagctga tttacctgaa
     9541 aaagttatta gcagagcaca agtgatccta agcgagtttg aagcgtctgc tggtaaaaaa
     9601 tcatcgatat caaatttaaa aatggtcgaa aatgaacctg aaattaatca agaaaattta
     9661 aacttaagtg ttgaagaaac aactgatact ttatctcaaa aagactttga acaagcatca
     9721 tttgatttgt ttgaaaatga tcaagaaagc gagattgaac tacaaattaa aaatttgaat
     9781 ttatctaata tgacaccaat tgaggcattg gtgaagttaa gtgaattaca aaatcaatta
     9841 aaatagaggt gttgcaaaat ggggaaaatt aaagaactcc aaacctcatt agcaaataaa
     9901 atcgcagcag gtgaagtagt tgaaagaccg agttctgttg tgaaagaact gttggaaaat
     9961 gctatagatg caggcgctac agaaataagc attgaagtag aggaatctgg cgtccaatct
    10021 attcgcgtag tcgataatgg aagcggaatt gaagcggaag acttaggatt agtatttcat
    10081 agacatgcga ctagtaaatt agatcaagat gaagatttat ttcatattag gacattagga
    10141 ttccgtggtg aagcactagc cagtatttca tcagttgcta aagtaacatt gaagacttgc
    10201 acggataatg ctaatggaaa tgaaatatat gtagaaaatg gtgaaatatt aaatcataag
    10261 cctgcaaaag cgaaaaaagg aacagatata cttgtagaat cattatttta taatacacca
    10321 gcacgtttaa aatatattaa aagtttatac actgaactag gtaaaataac agatattgtc
    10381 aacagaatgg caatgagcca tccggacatt cgaatagcac tcatttcaga tggcaaaaca
    10441 atgttaagta caaatggttc aggacgaact aatgaagtga tggcagagat ttatgggatg
    10501 aaagttgcac gagatttagt acatatatct ggagatacaa gtgattatca cattgaaggt
    10561 tttgttgcaa agcctgaaca ttctagaagt aataagcact atatttctat ttttattaat
    10621 ggacgataca ttaaaaactt tatgctaaat aaagcgattt tagaaggcta tcatacactc
    10681 ttaacaatag gtaggttccc gatttgttat attaatattg aaatggatcc aatcttagta
    10741 gacgtaaatg ttcatccaac aaaactagaa gtgcgtttat caaaagaaga gcaactatac
    10801 caattgattg tgagcaaaat acaagaagca tttaaagacc gtatattaat tcctaaaaat
    10861 aacttggatt atgtgccaaa aaaaaataaa gtattacatt cattcgaaca acaaaaaatc
    10921 gaatttgaac aaagacaaaa cacagagaat aaccaagaga agacgttttc atctgaagaa
    10981 agtaacagta agccatttat ggaagaaaat caaaacgatg agatagttat aaaagaagat
    11041 tcatataatc cattcgtaac gaaaacatct gaaagtttaa tagctgatga tgaatcttct
    11101 ggttataata atacacgtga aaaagatgaa gactacttca aaaaacaaca agaaatttta
    11161 caagaaatgg atcaaacatt tgattcaaat gatgatacaa ctgtgcaaaa ttatgagaat
    11221 aaagcgtctg atgattatta tgatgtaaac gatattaaag gaacaaaaag taaagaccct
    11281 aaacgaagaa ttccatatat ggaaattgtt ggacaagtac atggaacgta tattattgct
    11341 caaaatgaat ttggcatgta catgattgac cagcatgcag ctcaagaaag aataaaatat
    11401 gaatattttc gagataaaat aggtgaggtt accaatgaag tacaagattt attaatcccg
    11461 ttaacatttc atttttcaaa agatgaacaa ttagtcattg atcaatataa aaatgagctt
    11521 caacaagtag gtatcatgtt agaacatttt ggtggtcatg attatattgt aagtagctat
    11581 ccagtttggt tccctaaaga tgaagtagaa gaaattatta aagatatgat tgagctaatt
    11641 ttggaagaga aaaaagtaga tatcaaaaaa ttacgtgaag atgtagcaat catgatgtca
    11701 tgtaaaaaat ccattaaagc gaatcattat ttacaaaaac atgaaatgtc tgatttaatt
    11761 gatcaattaa gagaagcgga agatccattt acatgtccac atggtcgtcc aataatcatt
    11821 aatttttcaa aatacgaatt agaaaaatta tttaagcgtg tgatgtagag aggatgaatc
    11881 aagtgaataa caacatattg cctgctataa gaaacattaa agatttagag aaactgatta
    11941 aaacagatta taaaatgtgt gtgcttctag atatgcatat aggacatata aaaagtatta
    12001 tggaattgct gaagcaaaat catatagagt gttttattca tatagatttg ataaaaggtt
    12061 taagccacga tgaatttgca agtgaattta ttattcagca atacaagcca aaaggtatcg
    12121 tatcgactaa atctaaagta ataaaaaaag ctaaatcatt aaatacttta acgattttta
    12181 gagtatttat tattgatagt caagcattga aacgcagtat agatttgata aaaaaagttg
    12241 aacctgattt tgttgaagta cttccaggtg ttgcgagtaa agcgattcat catattcaga
    12301 aagaaacaaa cacacaagtc attgcaggtg gcctaattaa tacaatagat gaagtcaatg
    12361 aagctgttaa aaatggagcg aaatatgtaa caactagtta tgataaactt tggtaaatca
    12421 taagtctgca aaaatttcat ttttcacgag atttagtcat ataacataat aaaaaagccg
    12481 ttttcatatg ggatatgcag taggtatcat acaaaaaata atcaaataaa ataattagca
    12541 cctctaaaac gattaaaatc attaaagttt tagaggtgtt tattattaaa aaatgcaact
    12601 ggtcataaaa aatatggctg aagaatgtca attaaacata atggcgtcat aaataaatta
    12661 gttaggattg gtatttctag ttttcaaatt aaactacttt tataaagact tgaaaaatta
    12721 aatttgacaa aatttttaca aaaaccattg acaacgcttt catatcgata gtatcataac
    12781 aaatataata aaagttaata catagaatag agacgggaga tttctacgag ccaaactgct
    12841 agtgtaggaa tctctttgtc tttttgggag gacatttaat atgaatgtat atttagcaga
    12901 attcctagga actgcaatct taatcctttt tggtggtggc gtttgtgcca atgtcaattt
    12961 aaagagaagt gctgcgaatg gtgctgattg gattgtcatc acagctggat ggggattagc
    13021 ggttacaatg ggtgtgtttg ctgtcggtca attctcaggt gcacatttaa acccagcggt
    13081 gtctttagct cttgcattag acggaagttt tgattggtca ttagttcctg gttatattgt
    13141 tgctcaaatg ttaggtgcaa ttgtcggagc aacaattgta tggttaatgt acttgccaca
    13201 ttggaaagcg acagaagaag ctggcgcgaa attaggtgtt ttctctacag caccggctat
    13261 taagaattac tttgccaact ttttaagtga gattatcgga acaatggcat taactttagg
    13321 tattttattt atcggtgtaa acaaaattgc cgatggttta aatcctttaa ttgtcggagc
    13381 attaattgtt gcaatcggat taagtttagg cggtgctact ggttatgcaa tcaacccagc
    13441 acgtgattta ggtccgagaa ttgcacatgc gattttacca atagctggta aaggtggttc
    13501 aaattggtca tatgcaatcg ttcctatctt aggaccaatt gccggtggtt tattaggtgc
    13561 agtggtatac gctgtatttt ataaacatac atttaatatt ggttgtgcaa ttgcaattgt
    13621 tgtagttatt attactttga ttttaggtta cattttaaat aaatcatcaa aaaaaggtga
    13681 tatcgaatca atttactaaa ataaaaagaa acgtaaatag cataatttaa catgtttgat
    13741 tcatggatta tgctattttt tcgccaaaat ttaacagatt ttgtacaatg ggttagcgat
    13801 tattttttaa taaaggagat actactaatg gaaaaatata ttttatctat agaccaagga
    13861 acaacaagct caagagcgat tttattcaat caaaaagggg aaattgcagg ggtagcacaa
    13921 cgtgagttta agcaatattt tccacaatca ggttgggttg aacatgatgc aaatgaaatt
    13981 tggacatctg tgttagctgt aatgacggaa gtaattaatg aaaatgatgt tagagctgat
    14041 caaattgcag gtatcggtat tacaaaccaa cgtgaaacaa cggttgtttg ggacaaacat
    14101 actggccgcc caatttatca cgcaattgtt tggcaatcac gtcaaacaca atcaatttgt
    14161 tcagaattaa aacaacaagg atatgaacaa acatttagag ataagacagg attactttta
    14221 gatccgtatt ttgcaggtac aaaagttaaa tggattctag acaatgttga aggtgcacga
    14281 gaaaaagcag aaaatggcga tctattattt ggaacgattg atacttggtt agtatggaaa
    14341 ttatcaggaa aagctgcgca tattactgat tattcaaatg cgagtcgtac attaatgttt
    14401 aatatccatg atttagaatg ggacgatgag ttattagaac tacttacagt acctaaaaat
    14461 atgttgccag aagttaaagc ttcgagtgaa gtatatggta agacaattga ttaccacttc
    14521 tatggtcaag aagtaccaat cgctggagta gctggtgatc aacaagcagc attatttgga
    14581 caagcttgct tcgaacgtgg tgacgtgaaa aacacatatg gaactggtgg cttcatgtta
    14641 atgaatacag gtgacaaagc ggttaaatct gaaagtggtt tattaacaac aattgcttat
    14701 ggtattgatg gaaaagtaaa ttatgcgctt gaaggttcca tctttgtttc gggttcagca
    14761 atccaatggt tacgtgatgg attaagaatg attaattcag caccacaatc agaaagttat
    14821 gcgacacgag ttgactctac tgagggtgtt tatgttgttc cagcttttgt aggtttagga
    14881 acaccatatt gggattctga agcacgtggt gcgattttcg gtttaacacg tggaactgaa
    14941 aaagagcact ttatccgtgc aactttagaa tcattatgtt accaaactcg tgacgttatg
    15001 gaagcaatgt caaaagactc tggtattgat gtccaaagtt tacgtgtcga tggtggtgca
    15061 gttaaaaata actttattat gcagttccaa gcagacattg ttaatacttc tgttgaaaga
    15121 cctgaaattc aagaaactac agctttaggt gctgcatttt tagcaggatt agcagttgga
    15181 ttctgggaga gtaaagatga tatcgctaaa aactggaaat tagaagaaaa attcgatccg
    15241 aaaatggatg aaggcgaaag agaaaaatta tatagaggtt ggaaaaaagc tgttgaagca
    15301 acacaagttt ttaaaacaga ataaacttgt agattagact tttgtataaa cattgtgata
    15361 caatcaattt aagttaatat ttgaatcgag aaacgagaga tttgttcgaa catgtacaat
    15421 tgaaggattg tttatgtggg acaagtctct cgtttttaca ttttttagga ggcgttttgg
    15481 aatggcattg tctactttta agagagaaca tattaaaaag aatttaagaa atgatgaata
    15541 tgatttagta attattggtg gcggtattac aggtgcaggt attgcactag acgcgagtga
    15601 aagaggaatg aaagttgcat tagttgaaat gcaagacttt gcacaaggaa caagctcaag
    15661 atctacaaaa ttagtccatg gtggtttgcg ttacttaaaa caattccaaa ttggagtagt
    15721 tgccgaaact ggtaaagaac gtgcgattgt ttatgaaaat gggcctcatg ttacgactcc
    15781 agagtggatg cttttaccaa tgcataaagg tggaacattt ggtaaattct caacatcaat
    15841 tggtttagga atgtatgatc gtttagcagg tgttaagaag tctgaacgta aaaaaatgtt
    15901 atctaaaaaa gaaactttag ctaaagaacc attagttaaa aaagaaggtc taaaaggcgg
    15961 cggttactat gttgaatatc gtactgacga tgcgcgttta actattgaag ttatgaagcg
    16021 tgctgctgaa aaaggcgcag aaattatcaa ctatactaag tctgaacact tcacttatga
    16081 taaaaatcaa caagtaaatg gtgttaaagt tattgataaa ttaactaatg aaaattatac
    16141 aattaaggct aaaaaagtgg ttaatgcagc aggtccatgg gttgatgatg ttagaagtgg
    16201 tgattatgca cgcaataata aaaaattacg tttaactaaa ggtgtacatg ttgttattga
    16261 tcaatcaaaa ttcccattag gtcaagcagt atactttgat actgaaaaag atggaagaat
    16321 gatttttgca attccacgtg aaggaaaagc gtatgtaggt actacagata cattctatga
    16381 caatatcaaa tcttcaccat taactacaca agaagacaga gactatttaa tcgatgcgat
    16441 taattacatg ttccctagtg ttaatgttac agatgaagat attgaatcaa catgggcagg
    16501 aattagacca ttaatttacg aagaaggcaa agacccttct gaaatctctc gtaaggatga
    16561 aatttgggaa ggtaaatcag gtttattaac tattgcaggt ggtaaattaa caggctatcg
    16621 tcacatggct caagacattg ttgatttagt atctaaacgc ttgaaaaaag actacggttt
    16681 aacatttagt ccatgtaata caaaaggtct ggcaatttca ggtggcgatg taggaggtag
    16741 caagaacttt gatgcgtttg tagagcaaaa agtagatgta gctaaaggat tcggcattga
    16801 tgaagatgtt gcaagacgtt tagcatctaa atatggttca aatgttgatg aattgttcaa
    16861 cattgcgcaa acatctcaat accatgatag caagttacca ttagaaattt atgtagaact
    16921 tgtttatagt attcaacaag aaatggtata caaacctaac gatttcttag ttcgtcgttc
    16981 tggtaaaatg tacttcaata ttaaagatgt attagattat aaagatagta tcatcgatat
    17041 tatggctgat atgttagact actcaccagc tcaaattgag gcttatactg aagaagttga
    17101 gcaagcaatt aaagaagcgc aacatggaaa taatcagcca gcagttaaag aataattaat
    17161 ttgtacaatc ataaactggt gtcctgtttt aggggcatca gtttttttat atgagataca
    17221 ttagtcaatt gaaactatgc atcactaaat gtatgataat aataaaagcg aatataaaaa
    17281 cgttgttatt aaaggtgtga gatgatgact gaaaaacaat ttaaattaac tgtacaagat
    17341 aacacgaata ttgaagttaa agtgaatttt acagatgtag attcaaaagg aattattcat
    17401 atatttcatg gtatggctga acatatggaa cgttacgata aattagcaca tgcactttca
    17461 aaggatggct tcgatgtgat atgtcataat catcgaggac atggtattaa tattgatgaa
    17521 tcaacaagag ggcattacga tgatatgaaa cgagttatcg gtgatgcctt tgaagtagcg
    17581 caaacagtga gaggcaatgt tgataaacca tacattataa tcggacattc aatgggatcc
    17641 gttatagcta gattgtttgt agaaacatat ccgcaatatg ttgatggtct aattttaagt
    17701 gggacaggga tgtatccatt atggaaagga ttaccaaccg ttaaggtgtt acaactgatt
    17761 acaaaaattt atggtgctga gaaacgggtt gaatgggtta accagttagt atcaaatagt
    17821 tttaataaaa aaatacgtcc attacgtaca caaagtgatt ggatttctag taatccaact
    17881 gaagtagaca actttattaa agatccatat agtggattta atgtgtcaaa tcaattatta
    17941 tatcaaacag cctattatat gctacataca tcacaattaa aaaatataaa aatgttaaat
    18001 catgccatgc ctatattatt agtttcagga tgtgacgatc ctttaggtga ttatggtaaa
    18061 gggattttaa aattggcgaa tatatataga aaagctggca ttaaaaatgt taaagtgaat
    18121 ctttatcatc ataaacgtca tgaagtgtta tttgaaaaag atcatgacaa aatttgggaa
    18181 gacttgttta aatggttgaa tcaattttat aaaaaataaa gaaagtggaa ttaaatatga
    18241 ataaaaataa gccttttatt gtagtaattg tggggccaac tgcttcaggt aaaacagagc
    18301 ttagcataga actcgcgaag cgtatcaatg gtgaaatcat aagcggtgat tctatgcaag
    18361 tctacaaaca tatgaatatt ggaactgcaa aagtaacacc tgaagaaatg gatggtattc
    18421 cacatcattt aattgatatc ttgaatcctg atgatacatt ttcagcatat gaattcaagc
    18481 gattagcaga agatttaatt actgatataa cgaatagagg taaagttcca atcatagcag
    18541 gtggaacagg cttatatatt caatcattaa tatataatta tgaattagaa gatgaaacag
    18601 ttacacctgc acaattatcc atagttaaac aaaagttatc tgcattagaa catttagata
    18661 atcagcaact acacgattat ttagatcaat ttgatgcggt ttctgcagaa aatattcacc
    18721 ctaacaaccg ccaaagagta ttgcgcgcta ttgaatatta tttaaaaaca aaaaaacttt
    18781 tgagtaatcg caagaaagtg caacaattta ctgaaaatta tgatacatta ttattaggga
    18841 ttgaaatgtc gcgcaaaaca ttatattcaa gaataaataa acgtgttgat attatgttgg
    18901 atcacggatt atttagagaa gtgcaacaac ttgttgaaca aggctatgaa tcttgccaaa
    18961 gtatgcaagc tattggatat aaagaattaa tatctgtgat taacggacaa atgatttatg
    19021 aagatgctgt caatgattta aagcaacatt cacgccaata tgcaaaacga caaatgacat
    19081 ggttcaagaa taaaatgagt gttcattggt tagataaaga aaatatgtca cttcaaatga
    19141 tgttagatga gattacaacc cagattaagt aaaaggagtc cgacagatga ttgcaaacga
    19201 aaacatccaa gacaaagcac tagagaattt taaagcaaac caaactgaag taactgtatt
    19261 ctttctaaac ggtttccaaa tgaaaggtgt tattgaagaa tacgacaagt atgtcgtaag
    19321 cttaaattct caaggcaaac aacacttgat ttacaaacat gcgatcagca cttatacagt
    19381 agaaactgaa ggtcaagcat ctactgaaag tgaagaataa gttgtaatca tatgggcacg
    19441 atttaatgac tcgtgtacat aacgtttgaa gtatgataaa tagatataga aataaagtca
    19501 tatgtgacat caattaaatg atgttcaaat gacaagatac aatagaggaa tgtttgtaat
    19561 aaaaacgctt catataatgg tcgagtcaat atatgatacg actttatatg aagcgtttta
    19621 tttgctatga gctagtatat tttataataa cttttctatt tctctttcga tttgaacagg
    19681 ttttttttga ggtgcaaatc gtttaacaac gttaccttcg cgatccacta aaaacttagt
    19741 gaaattccat ttgattttct cattaaagaa tccatgttgt gccgcagtca aatatctaaa
    19801 taaaggtaat tgatgttccc cttttacgtc tattttttga tgcataggga aggtaacacc
    19861 atagtttaat ttacagtttt gagctgcttc ttcgcctgat ccaggttctt ggccaccaaa
    19921 ttgattacaa gggaaaccta gaattacaaa cccttgatct ttgtatttct cgtataatga
    19981 ttgcaaacct tcaaattgtg aagtaaagcc acattcgcta gctgtattaa caattagcat
    20041 aacgtcaccc ttatatgcat ctaatttgta agtaacacct ttatttgttt ctactacaaa
    20101 atcataaatt gtctccattg aatcatcctt tcgatttact taaaatgtac cataaaatcg
    20161 tgtaatagtc tttactaaaa ctctatgata gaatactttg agtaggattt tattaaggag
    20221 atgtataaca tggctcagca acaaattcat gatattaaaa ttaaactaga aaaagctgtc
    20281 ttagtcggtg tacatgctca agatgataag caatttaatt ttgagtctac aatggaggaa
    20341 ttatcatctt tatcagagac ttgccaactt gaagtgttgg gtcaaattac tcaaaacaga
    20401 gatcgtgtag atcgcaaata ttatgttggt aaaggtaaaa ttgaagaaat tcaagcattt
    20461 attgagttca aagatattga tgtagtcatc acaaatgatg aattaacgac tgcacaatcc
    20521 aaatcactaa atgaagcttt aggtgtaaaa attattgata gaactcagtt gattcttgaa
    20581 atatttgcat taagagcaag aagtaaagaa ggtaaattgc aagtagagct agcacaactt
    20641 gattatttat tacctagatt gcaaggccat ggtaaaagcc tttctcgttt aggtggcggt
    20701 attggaacta gaggccctgg tgaaacgaag ttagagatgg atcgcagaca tattcgaact
    20761 cgtatgaatg aaattaaaca tcaattgcgg acggtagaag aacatcgcga aagatatcga
    20821 aataaaagaa atcaaaatca ggtgtttcaa gtagctttag ttggttatac aaatgctggt
    20881 aaatcatcat ggtttaatgt tttagcaaat gaagagacgt atgaaaaaga tcaattattt
    20941 gcaacgttag atcctaaaac acgacaaatt caaataaatg atggatttaa tttaattatt
    21001 tcagatactg ttggttttat acagaaacta cctacgacgt taattgcagc ttttaaatca
    21061 actttagaag aggctaaagg tgcggattta ttagtacatg tcgtagatag tagccatcct
    21121 gaataccgta cacagtatga cacagttaat gatttaatca aacaattaga tatgagtcat
    21181 atttctcaaa tagttatttt taataaaaag gacttatgtg atcatgcatc aaatcgtcca
    21241 gcaagtgatt tgcctaatgt ttttgtttct tctaaaaatg atggtgataa attacttgtt
    21301 aagacgttat ttattgatga aatcaaaagg cgattaactt attatgatga ggcaattgcg
    21361 acgaataatg cagatcgatt atattttcta aaacaacata cattagtgac tgaacttaaa
    21421 tatgatgaaa ttgaaaatgt ttatcgtata aaaggattta aaaaataata aaaggacgaa
    21481 attcaaatga aagatataag taagatagta gctgacgtcg aatcaacgtt agcaccatat
    21541 tttaaagaaa ttgaagaaac agcatatatt aatcaagaaa aagtattaaa tgcgtttcat
    21601 catgtcaaag caaccgaaag tgatttacaa ggatcaacag gttacgggta tgatgatttt
    21661 ggacgtgacc atttagaaga aatatatgcg caggcattta aagcagaaga tgcaattgtt
    21721 cgtccgcaaa ttatttcagg tacgcatgcg attactattg cattacaaag tttattaaaa
    21781 catggtgatg aattaattta tataacgggt agtccatatg acactttact tgaagtcatt
    21841 ggcgtaaacg gaaatggtat tgaaagttta atggagtacg gcgtatcgta taaagatatt
    21901 gcacttaaag aaggtaaaat cgatattgaa agtgtgttag atggggtttc tgagcgcacc
    21961 aaagtaatag cgattcaacg ttcgaaaggc tatgatcaaa gaccttcaat tcctctagat
    22021 gaaattgaac aggtgattat taggttgaaa gaagtgcatc ctaatatttt aatatttgtg
    22081 gataactgtt atggggaatt tgtagaaaga cgtgaaccta tagaatgtgg tgccgattta
    22141 atagcaggat cattaattaa aaaccctggc ggtggtttag ctaagattgg tggatacatt
    22201 gctggtagaa aagatttaat tgaacgatgt ggttatagat tgacagcacc tggtattggt
    22261 aaagaagcag gtgcatcatt aaatgcattg cttgaaatgt atcaaggttt ctttttagca
    22321 ccacatgttg tcagtcagag tcttaaaggg gcattgttta ctagtttatt tttagaaaaa
    22381 atgaatatga acacaacgcc gaagtactac gaaaaacgaa ctgatttaat tcaaacagtt
    22441 aaatttaaaa cgaaagaaca aatgatttca ttttgtcaaa gtattcaaca cgcatcccca
    22501 attaatgcac attttagtcc agaaccgagt tatatgcctg gttacgaaga tgatgttatt
    22561 atggctgctg gtacgtttat tcaaggttca tcgattgaat tatctgcaga tggacctatt
    22621 cgtcctcctt atgaagcgta tgttcaagga ggattaacat atgaacacgt taaaattgct
    22681 gtgacaagag ctgttaatca gttgaaagaa caaggactta tataatagat taaatttcac
    22741 cctaaatgat aatgtatttt gggtgaaatt tttttgcgga gatttatcag attattttta
    22801 aaaattgaaa aaatatttta gctgtagatt gttttatggc gttttaaagg tcaaaattgc
    22861 agttatatca atgttttgag ttttatgtca gaaaacctga catatttttg aaatcctaaa
    22921 aaaattatga taagttatta acaagttcaa aagtagagga gaggaacaat gatatcgaat
    22981 gatgcaatca gacgaaatat ggctgtcttc tctatgagtg tagtaagtaa attaacggat
    23041 ttaacgccaa ggcaaatacg ttactatgaa acacatgaac tcatcaaacc tgaaagaaca
    23101 gaaggtcaaa aacgtctgtt ctcactcaat gatttggaaa gattactaga aattaaatca
    23161 cttttagaaa aaggatttaa tatcaaaggg attaaacaaa tcatttatga ctcacaagag
    23221 catttaacaa cagatgaaca agagataaga aaaaagatga ttgtagatgc cacgcaaaag
    23281 cctattggag aaactttacc aataaatcgt ggtgatttat cccgatttat taaataaaat
    23341 ttggaggatt ttaaaatgcc aaaacgtact ttcactaaag acgacattcg taaatttgca
    23401 gaagaggaaa atgtaagata tttaagatta caattcactg atattttagg aacaattaaa
    23461 aatgttgaag tgcctgtaag ccaattagaa aaagtacttg ataacgaaat gatgtttgac
    23521 ggttcttcta tcgaaggttt cgtacgtatc gaagaatctg acatgtattt acatccagat
    23581 ttagatactt gggtaatctt cccatggact gctggtcaag gtaaagtagc acgtttaatt
    23641 tgtgatgtat ataaaacaga tggtacgcca tttgaagggg atccacgtgc aaacttaaaa
    23701 cgtgtattaa aagaaatgga agatttaggc ttcacagact ttaacctagg gcctgaacca
    23761 gaattcttct tgtttaagtt ggatgaaaaa ggagaaccaa ctttagaact taatgatgat
    23821 ggtggatatt tcgatttagc acctacagat ttaggagaaa actgtcgtcg tgatattgtt
    23881 ttagaattag aggatatggg cttcgatatt gaagctagtc accatgaagt tgcaccgggt
    23941 caacatgaaa tcgactttaa atatgcggat gcagttacag catgtgataa catccaaaca
    24001 tttaaattgg ttgttaaaac aattgcacgt aagcataatt tacatgcaac attcatgcct
    24061 aaaccattat ttggtgtgaa cggtagcggt atgcacttta atgtttcatt attcaaagat
    24121 aaagaaaatg cattctttga tccgaatact gaaatgggct taacagaaac agcttaccaa
    24181 ttcacagctg gtgtacttaa aaacgcacgt ggatttacag cggtatgtaa cccattagta
    24241 aactcataca aacgtttagt tcctggttat gaagcaccat gttatattgc atggagcggt
    24301 aaaaaccgtt caccgttaat ccgtgtacca tcttcaagag gattatcaac tcgtattgaa
    24361 gtacgttcag ttgacccagc tgcgaaccca tatatggcat tagctgcaat tttagaagct
    24421 ggtttagacg gtattaaaaa taaattaaaa gttccagaac ctgttaacca aaatatttac
    24481 gaaatgaatc gtgaagaacg tgaagcagta ggcattcaag acttaccttc aacactttac
    24541 acagcattaa aagcaatgcg tgaaaatgaa gttattaaaa aagctttagg aaatcatatc
    24601 tataatcaat ttattaattc aaaatcaatt gaatgggatt actacagaac tcaagtatct
    24661 gaatgggaaa gagatcagta catgaagcaa tattaatacg ctaaacaatg atgggaaatc
    24721 agtaatatca agggctagag cgttttgctt tagctctttt ttgatttgga aagatgggat
    24781 ttcacttgac cacttttgtt aaaaaatgga tttggtcaag ttttggtcaa gtaaatgttt
    24841 tatgtattta tttttttaaa gctggggaag accattttat attttagttc acatgaattt
    24901 taatatattt ggaggtgatg agatgaggcg ataaaagaac gtcgcgaaaa cgttgagtat
    24961 tacttgaaaa tagccatatt catattactt ctcatcgaat tcatttgcag aacgttctaa
    25021 ggtaagaccc cgaaaagagg gaccttacta cgcattatta agggggtaat actatatgaa
    25081 aagagaaaca aaacaaaaaa tatctttttg tttatcgatc ggtattttca ttttagttat
    25141 actactattg atattctaaa aaattaaaaa tttctcctca tctaatttat gaattgctat
    25201 gatgaaatat tcaatacaat caaagaattg atagaaaaca aagagatatc gagttatcaa
    25261 attaataaag atactgggat aagttacggt aatattaatg ctatgcgccg tagagaaaga
    25321 agaatagaaa atttaagctt aaagaatgca aagatcttat atgaatatgc gaaaaaggta
    25381 ttgtaatata agcattgagt tcaataggca tacaataact gcccaaacta gtgactgagt
    25441 gggtgctttt atagtattaa aagttttcta aaagtgaaag aaatcatgtt tagtgattta
    25501 cttatgtttt tcaaaatagt ataataattt tatacaatat atagagcttc agtatacctt
    25561 ttgagtatat atggagttct ttctttttat acaagatgta tatgtataaa tggaaatatt
    25621 taatacatta cataaaaacc tttaggtgca cttagaggga tgttaatcat aaaaaaagag
    25681 attttgaaaa atataggcta ttatacttta gaattacaaa ttataaatct tgccagtgga
    25741 tatgggagtt caacatgtca cttaccccaa tttgtggaat gttaaataac tataaagagg
    25801 aaaggagaaa aaatgataag aatggttaaa gaaaaagaac gaatagcaaa attaaatttc
    25861 gaatagctgt gacgagctta ataattcaag tacttaattt tatcaaatcg ttcttttaaa
    25921 aaggtaaagg gcaaaagacc tttaccacat taaataggga ggtggtattt atgacaagag
    25981 aattaaggaa aaaatta
//
LOCUS       DAHHOJ010000027        25633 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.027, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000027 DAHHOJ010000000
VERSION     DAHHOJ010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.027"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(38..241)
                     /locus_tag="NBH32_000968"
                     /pseudo
     CDS             complement(38..241)
                     /locus_tag="NBH32_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789088.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(278..1018)
                     /locus_tag="NBH32_000969"
     CDS             complement(278..1018)
                     /locus_tag="NBH32_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCD1514588.1"
                     /translation="MFNLENDLETLKHIIDSSNRITFFTGAGVSVASGVPDFRSMGGL
                     FDEISKDGLSPEYLLSRDYLEDDPEGFINFCHKRLLFVDTKPNIVHDWIAKLERNQQS
                     LGVITQNIDGLHSDAGSQHVDELHGTLNRFYCNACHKSYTKSDVIDRTLKHCDNCGGA
                     IRPDIVLYGEMLDQPTIIRALNKIEHADTLVVLGSSLVVQPAAGLISNFKGDNLIIIN
                     KDRTPYDSDATLVIHDDMVSVVKSLITG"
     gene            1224..2015
                     /locus_tag="NBH32_000970"
     CDS             1224..2015
                     /locus_tag="NBH32_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1514589.1"
                     /translation="MTHRLNKGGNFTLNKHERLDEIAKLVNKKGTIRTNEIVEGLNVS
                     DMTVRRDLIELENKGILTKIHGGARSNSTFQYKEISHKEKHTRQIAEKRFIARKAASL
                     IEDGDTLFFGPGTTVELLAEEVNHHTLTIITNCLPVYKILLEKQTAHFRVYLIGGEMR
                     HITEAFVGEMANAMLEKLRFSKMFFSSNAVNKGAVMTSTLDEAYTQQLALSNSIEKYL
                     LIDHTKVGKEDFTSFCQLNELTAVVMDYEDEEKVETIKTYIEVVD"
     gene            2383..2811
                     /gene="lacA"
                     /locus_tag="NBH32_000971"
     CDS             2383..2811
                     /gene="lacA"
                     /locus_tag="NBH32_000971"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HCD1514590.1"
                     /translation="MAIIIGSDEAGKRLKEVIKSYLLDNKYDVVDVTEGQEVDFVDAT
                     LAVAKDVQSQEGNLGIVIDAFGAGSFMVATKIKGMIAAEVSDERSGYMTRGHNNSRMI
                     TMGSEIVGDTLAKNVVKGFVEGKYDGGRHQIRVDMLNKMC"
     gene            2827..3342
                     /gene="lacB"
                     /locus_tag="NBH32_000972"
     CDS             2827..3342
                     /gene="lacB"
                     /locus_tag="NBH32_000972"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HCD1514591.1"
                     /translation="MKIALGCDHIVTDTKMRVSEFLKSKGHEVIDVGTYDFTRTHYPI
                     FGKKVGEQVVSGNADLGVCICGTGVGINNAVNKVPGVRSALVRDMTSALYAKEELNAN
                     VIGFGGRIIGELLMCDIIDAFINAEYKPTEENKKLIAKIKHLETSNADQADPHFFDEF
                     LEKWDRGEYHD"
     gene            3355..4287
                     /gene="lacC"
                     /locus_tag="NBH32_000973"
     CDS             3355..4287
                     /gene="lacC"
                     /locus_tag="NBH32_000973"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HCD1514592.1"
                     /translation="MILTLTLNPSVDISYPLTALKLDDVNRVQEVSKTAGGKGLNVTR
                     VLAQVGEPVLASGFIGGELGQFIAKKLDHAGIKHAFYNIKGETRNCIAILHEGQQTEI
                     LEQGPEIDNQEAAGFIKHFEQLLEKVEAVAISGSLPKGLNQDYYAQIIERCQNKGVPV
                     ILDCSGATLQTVLENPYKPTVIKPNISELYQLLNQPLDESLESLKQAVSQPLFEGIEW
                     IIVSLGEQGAFAKHNHTFYRVNIPIISVLNPVGSGDSTVAGITSAILNHENDHDLLKK
                     ANTLGMLNAQEAQTGYVNLNNYDDLFNQIEVLEV"
     gene            4291..5271
                     /gene="lacD"
                     /locus_tag="NBH32_000974"
     CDS             4291..5271
                     /gene="lacD"
                     /locus_tag="NBH32_000974"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HCD1514593.1"
                     /translation="MSKSNQKIASIEQLSNNEGIISALAFDQRGALKRMMAKHQTEEP
                     TVAQIEQLKVLVAEELTQYASSILLDPEYGLPASDARNKDCGLLLAYEKTGYDVNAKG
                     RLPDCLVEWSAKRLKEQGANAVKFLLYYDVDDTEEINIQKKAYIERIGSECVAEDIPF
                     FLEVLTYDDNIPDNGSVEFAKVKPRKVNEAMKLFSEPRFNVDVLKVEVPVNMKYVEGF
                     AEGEVVYTKEEAAQHFKDQDAATHLPYIYLSAGVSAELFQETLKFAHEAGAKFNGVLC
                     GRATWSGAVQVYIEQGEDAAREWLRTTGFKNIDDLNKVLKDTATSWKQRK"
     gene            5293..5604
                     /locus_tag="NBH32_000975"
     CDS             5293..5604
                     /locus_tag="NBH32_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HCD1514594.1"
                     /translation="MNREEVQLLGFEIVAFAGDARSKFLEALTAAQAGDFAKADALIE
                     EGNNCIAEAHRAQTSLLAKEAQGDDIAYSVTMMHGQDHLMTTILLKDLMKHLLEFYKR
                     G"
     gene            5610..7322
                     /locus_tag="NBH32_000976"
     CDS             5610..7322
                     /locus_tag="NBH32_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HCD1514595.1"
                     /translation="MMQKLIAQIEKGKPFFEKLSRNIYLRAIRDGFISAMPVILFSSI
                     FLLIAYVPNIFGFKWDKGMEAILMKPYNYTMGLVAFLVAGTTAKSLTDSFNRKLESTN
                     QINFISTMLAAMCGFLFLASDPAKDGGFLSAFMGTKGLLTAFLSAFVTVIVYNFCVKR
                     NITIKMPKEVPPNISQVFKDLIPFSAVIIILYALDLVIRNSFKSNVAEGILKLFEPLF
                     TAADGWIGVTIIFGAFALFWFVGIHGPSIVEPAIAAITYANIEANFKLLQAGEHADKI
                     ITSGTQMFIVTFGGTGATLVVPFMFMWMTKSKRNKAIGRASVVPTFFGVNEPILFGAP
                     LVLNPVFFIPFVLAPIVNVWIFKLFVEVLGMNSFSVNLPWTTPGPLGIIMGTGFGLWS
                     FVLAITLIVVDIIIYYPFLKVYDSEILDEEEGRKESNSDLKEKVAANFDTKKADSILA
                     ASGVSDDAAKASNITEQTNVLVLCAGGGTSGLLANALNKAAEEYHVPVKAAAGGYGAH
                     MDIMKEYQLIILAPQVASNYEDIKQDTDRLGIKLAKTQGAEYIKLTRDGQAALDFVQQ
                     QFEN"
     gene            7340..8752
                     /gene="lacG"
                     /locus_tag="NBH32_000977"
     CDS             7340..8752
                     /gene="lacG"
                     /locus_tag="NBH32_000977"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HCD1514596.1"
                     /translation="MTKTLPEDFIFGGATAAYQAEGATNTDGKGRVAWDTYLEENYWY
                     TAEPASDFYNRYPVDLELSEKFGVNGIRISIAWSRIFPNGYGEVNPKGVEYYHKLFAE
                     CHKRHVEPFVTLHHFDTPEVLHKDGDFLNRKTIDYFVDYAEFCFKEFPEVKYWTTFNE
                     IGPIGDGQYLVGKFPPGIKYDFEKVFQSHHNMMVAHARAVKLFKDGGYQGEIGVVHAL
                     PTKYPFDPSNPEDVRAAELEDIIHNKFILDATYLGKYSRETMEGVQHILSVNGGKLNI
                     TDEDYAILDAAKDLNDFLGINYYMSDWMRGYDGESEITHNATGDKGGSKYQLKGVGQR
                     EFDVDVPRTDWDWMIYPQGLYDQIMRVVKDYPNYHKIYITENGLGYKDEFIESEKTVH
                     DDARIDYVRQHLNVIADAIKDGANVKGYFIWSLMDVFSWSNGYEKRYGLFYVDFETQE
                     RYPKKSAYWYKELAETKEIK"
     gene            9018..9887
                     /locus_tag="NBH32_000978"
     CDS             9018..9887
                     /locus_tag="NBH32_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000719336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1514597.1"
                     /translation="MKKIFVIITTLLAVAIIIGSIITVVFSQRQAQTFKIQQQQFVKK
                     PIPTLFLHGFGGSANSEKFMVKQAEKRGVTKDIITAYVSKDGAVTFKGKLRKDAVNPI
                     VKIELENNRQGYLDKNAAWFKNVLTKLQSEYNFDKFNFVGHSMGNLTFAQYMMTYGND
                     KSLPQLNKQVNIAGTFNGVLNMNEDVNEITVDKDGKPSRMNQPYQQLRVLKDIYKGKG
                     IEVLNIYGDLKDGTHSDGRVSNSSSKSLKYLLGNSPKSYRESKYEGEPAQHSQLHENE
                     NVANELIDFLWKK"
     gene            10170..11171
                     /locus_tag="NBH32_000979"
     CDS             10170..11171
                     /locus_tag="NBH32_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCD1514598.1"
                     /translation="MQNKQILFNKIPEGMPQEDTFKIEEIDTPKLESDGVLVQTLYIS
                     VDPYMRGRMTKADSYVQPFEIGKPIVSHVVAKVIDSTLADYKKGDVVVGMLPWRIINH
                     VQADQITKVPTTDVPLDLYLSVLGMPGQTAYHGLLDIGQPKAGDTVVVSAASGAVGSV
                     VGQIAKIKGCRVVGIAGGDKKVNYLTETLGFDAGIDYKKEDFAEALAQAVPDGIDVYF
                     ENVGGTVGDEVFKHLNRFARVPVCGAISSYNHPEADIGPRIQGTLIKKQVMMRGFLVS
                     EFANAFKEASEQLATWVQEGKIQSQVTIEDGFENAPHAFKNLFTGDNFGKQVIKVTE"
     gene            11405..12412
                     /locus_tag="NBH32_000980"
     CDS             11405..12412
                     /locus_tag="NBH32_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HCD1514599.1"
                     /translation="MKMIGFEKPFKLEEGNLFKVYEQRKPTPENDDILVKVNSISVNP
                     VDTKQRQMEVTQAPRVLGFDAIGTVEAIGPDVTLFSPGDVVFYAGSPNRQGSNATYQL
                     VSEAIVAKAPHNISANEAVSLPLTGITAYETFFDTFKISHNPSENVGKSVLIINGAGG
                     VGSIATQIAKRYGLTVITTASRQETTEWCEKMGADIVLNHKEDLVRQFKEKEIPLVDY
                     IFCTYNTDLYYNTMIELIKPLGHITTIVAFNEDQDLNALKLKSITFTHEFMFARPIHR
                     TPDMIKQHEYLEDITKNIESGHYQPTTTQVFEGLSPENLYQAHLLLEKQSMIGKLVIN
                     I"
     gene            12719..14281
                     /locus_tag="NBH32_000981"
     CDS             12719..14281
                     /locus_tag="NBH32_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1514600.1"
                     /translation="MKNKSVSLVFWVSLVICTIFVAFGAIFPKQLEKLTQNITTFIAL
                     HFSWYYLLLVLVILFVCVYILFSRYANITLGEEGEDPEFSLPSWFAMLFSAGMGIGLV
                     FWTTAEPISHAFKLTPIHKAGTQSAINDAMQFSFFHWGIHAWAVYGIVALVFAYFSFH
                     KGYPGLVSATLTPLLGEKAMRGPLGGAIDVLAVIATVTGVAATLGFGALQINEGLHFL
                     FNVPSNFTMQVILIVITTILFTWSAWSGIDKGIKTLSNINMLLAFVVLIGLFIVGPTL
                     YILNTFTNGLGNYIANFFSMSLRIPSGGQKFQWLQNWTIFYWAWWISWAPFVGIFIAR
                     VSKGRTIKEFILGVLFVPALVCFIFFAVFGASAIYLQDNHIADIAKAATETATFATLQ
                     HYPLGFVLSLITLLVIMIFFVTSADSATYVLGMLSSRGDINPKSFVKVSWGIIMALFA
                     IIMIYTGGTQAIQNLLIIAALPFSVVIIAMIWSLLKSLSEEKPRNSNKVLIKHRDPDI
                     LEYRSSKHIDEN"
     gene            14456..14992
                     /gene="amaP"
                     /locus_tag="NBH32_000982"
     CDS             14456..14992
                     /gene="amaP"
                     /locus_tag="NBH32_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HCD1514601.1"
                     /translation="MKRLKNFILGLLIVVIVGFLLFMYIQDGRITEYQDYFLQFEWFQ
                     PLLISLAALLILIGLILVFSIFKPTHRKPGLYKDFDDGHVYVSRKAVEKTAFDTVAKY
                     DQVRQPNVVAKLYNKKNKSYIDIKTDFFVPNNVQVQSLTEAIRSDIKKNVEYFTEMPV
                     RKLEVNVRDQKTSGPRVL"
     gene            15005..15244
                     /locus_tag="NBH32_000983"
     CDS             15005..15244
                     /locus_tag="NBH32_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HCD1514602.1"
                     /translation="MANNHNQNGQDSTQQVINFLKVFKWRIVGFLAFLLIAILFLTLG
                     FWKTILIIVLCLIGVGIGYMKDRKQDFMNFLNRWS"
     gene            15307..15816
                     /locus_tag="NBH32_000984"
     CDS             15307..15816
                     /locus_tag="NBH32_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1514603.1"
                     /translation="MTVDNNKAKQAYDNQTGVNEKEREERQKQQEQNQEPQFKNKLTF
                     SDEVVEKIAGIAAREVKGILDMKGGLTDTFTNAFSSGNNVTQGVSVEVGEKQAAVDLK
                     VILEYGESAPKIFRKVTELVKEQVKYITGLDVVEVNMQVDDVMTQKEWKQKHEKNNEN
                     NNQERQGLQ"
     gene            complement(15977..17953)
                     /gene="sfaD"
                     /locus_tag="NBH32_000985"
                     /gene_synonym="sfnaD"
     CDS             complement(15977..17953)
                     /gene="sfaD"
                     /locus_tag="NBH32_000985"
                     /gene_synonym="sfnaD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine--citrate ligase SfaD"
                     /protein_id="HCD1514604.1"
                     /translation="MNLNLIFKEQTLKFNKEEQETYLFLQQHNNDWANIFKEMILQGR
                     DKVTQRLVTSMHRENLVKARTQSKKILSRDLIMLDISTTHILEIQFPQAKQTLYAPIT
                     GEHAFDRIDVEGPFYIKDDITNTITRVHHPNEILECILIEAPDLKNAASDQFQQDLIN
                     SATNMTFAISYQALSMQHDSTPLFNIIENSEDSYLRSEQAVIEGHPLHPGAKLRKGLN
                     ALQTFLYSSEFNQPIKLKIVLIHSKLSRTMSLSKDYDTTVHQLFPDLIKQLENEFTPK
                     FNFNDYHIMIVHPWQLDDVLHSDYQAEVDKELIIEAKHTLDYYAGLSFRTLVPKYPAM
                     SPHIKLSTNVHITGEIRTLSEQTTHNGPLMTRILNDILEKDVIFKSYASTIIDEVAGI
                     HFYNEQDEADYQTERSEQLGTLFRKNIYQMIPQEVTPMIPSSLVATYPFNNESPVVTL
                     IKRYQSAASLSDFESSAKSWIETYSKALLGLVIPLVTKYGIALEAHLQNAIATFRKDG
                     LLDTMYIRDFEGLRIDKAQLNEMGYSTSHFHEKSRILTDSKTSVFNKAFYSTVQNHLG
                     ELILTISKASNDSNLERHMWYIVRDVLDNIFDQLVLSTHKSNQVNENRINEIKDTMFA
                     PFIDYKCVTTMRLEDEAHHYTYIKVNNPLYRENN"
     gene            18055..19248
                     /gene="sfaA"
                     /locus_tag="NBH32_000986"
     CDS             18055..19248
                     /gene="sfaA"
                     /locus_tag="NBH32_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A export MFS transporter"
                     /protein_id="HCD1514605.1"
                     /translation="MTKYFFSSSFLLFLGNWIGQIGLNWFVLTTYHNAVYLGIVNFCR
                     LVPILLLSVWAGAIADKYDKGRLLRITISSSFLVTAILCVLTYSFTAIPISIIIIYAT
                     LRGILSAVETPLRQAILPDLSDKISTTQAVSFHSFIINICRSIGPAIAGVILAVYHAP
                     TTFLAQAICYFIAVLLCLPLHFKVTKMPEDATRYMPLKVIIDYFKLHMEGRQIFITSL
                     LIMATGFSYTTLLPVLTNKVFPGKSEIFGIAMTMCAIGGIIATLVLPKVLKYIGMVNM
                     YYLSSFLFGIALLGVVFHNIVIMFICITLIGLFSQWARTTNRVYFQNNVKDYERGKVL
                     SIIMMDRGMIPLGSLLMSICADVFGIVRTFSIMGISTICITMVFYFINRKLKLKLEES
                     NHGIS"
     gene            19235..20992
                     /gene="sfaB"
                     /locus_tag="NBH32_000987"
     CDS             19235..20992
                     /gene="sfaB"
                     /locus_tag="NBH32_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A synthetase SfaB"
                     /protein_id="HCD1514606.1"
                     /translation="MVYLEWAKADRNIQYRVINAIIKERIYPEQTFISQKGSLIEIQY
                     HMHVLTIEVVRKSALERYEFTGDITYLNKGETSLIITLEGLLDVLNHDFDIPISERLR
                     EELIHSRDSLVETYKQMSHRQTLISQSFKFSRLPQDINFFSWLQHVKDSDKTDDLTYS
                     ESLVPEGHPTHPLTKTKLPLTMEEVRAYAPEFEKEIPLQIMMIEKDHVVCTAMDGNDQ
                     FIIDEIIPEYYNQIRVFLKSLGLKNEDYRAILVHPWQYDHTIGKYFEAWIAKKILIPT
                     PFTILSKATLSFRTMSLIDKPYHVKLPVDVQATSAVRTVSTVTTVDGPKLSYALQNML
                     NQYPGFKVAMEPFGEYANVDKDMARQLACIIRQKPEIDGKGATVVSASLVNKNPIDQK
                     VIVDSYLEWLNQGITKESITTFIERYSQALIPPLIAFIQNYGIALEAHMQNTVVNLGP
                     HFDIQFLVRDLGGSRIDLETLQHRVSDIKITNDSLIADSIDAVIAKFQHAVIQNQMAE
                     LIHHFNQYDCVEETELFNIVQQVVAHAINPTLPHANELKDILFGPTITVKALLNMRME
                     NKVKQYLNIELDNPIKKEV"
     gene            20999..22069
                     /gene="sfaC"
                     /locus_tag="NBH32_000988"
     CDS             20999..22069
                     /gene="sfaC"
                     /locus_tag="NBH32_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin A biosynthesis protein SfaC"
                     /protein_id="HCD1514607.1"
                     /translation="MAHVNINISKIKYNAKVLQTVFQSKNMQFTPVIKCIAGDRTIVE
                     SLKALGINHVAESRLDNIISIADQDLTYTLLRTPAKKEISDMIEKVDMSIQTELSTIH
                     QINEVAEVLGKKHKILLMIDWKDGREGVLTYDVLDYIKEIIHLKNIHFVGLAFNFMCF
                     KSDAPSDDDIFMINRFVSAVEREIGYRLKIISGGNSSMLPQLLYNDLGKINELRIGET
                     LFRGVDTTTNQAIAMLYQDAITLEAEILEIKPRVNTQTHESFLQAIVDIGYLDTKVDN
                     ISPMDQHINILGASSDHLMLDLNGQGHYQVGDHISFSLNYEALSHSMYMKNLHKVYID
                     DSKIDTLLQNFDVKSPAMVNQY"
     gene            complement(22170..22295)
                     /locus_tag="NBH32_000989"
     CDS             complement(22170..22295)
                     /locus_tag="NBH32_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514608.1"
                     /translation="MYHGYIIFALMQLTPFSMTVIPVIQLILSVAILCDELLHFI"
     gene            22462..23445
                     /locus_tag="NBH32_000990"
     CDS             22462..23445
                     /locus_tag="NBH32_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1514609.1"
                     /translation="MRGLKTFSILGLIVALFLVAACGNTDNSSKKESSTKDTISVKDE
                     NGTVKVPKDAKRIVVLEYSFADALAALDVKPVGIADDGKKKRIIKPVREKIGDYTSVG
                     TRKQPNLEEISKLKPDLIIADSSRHKGINKELNKIAPTLSLKSFDGDYKQNIDAFKTI
                     AKALDKEKEGEKRLAEHDKLINKYKDEIKFDRNQKVLPAVVAKAGLLAHPNYSYVGQF
                     LNELGFKNALSDDVTKGLSKYLKGPYLQLDTEHLADLNPERMIIMTDHAKKDSAEFKK
                     LQEDATWKKLNAVKNNRVDIVDRDVWARSRGLISSEEMAKELVELSKKEQK"
     gene            23457..24488
                     /locus_tag="NBH32_000991"
     CDS             23457..24488
                     /locus_tag="NBH32_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1514610.1"
                     /translation="MAIKEISSQSAIDHKRKRRTTLTYIVSLCFLFICIYLNMAIGSS
                     KINFSDIVHYVTGHTDTKATFLLHNVRMPRMIAGLFIGGALAVSGLLMQAMTRNPLAS
                     PKIFGVSSGASFVIVLVTIIIPSLEYYALYLGVIGAFIGGLTVYTLSGATKGMTPIKL
                     ALAGMAIHLFFSSMTEGIIILNENSNEQVMFWLVGSLSSMKWDEILTILPWLIGALIA
                     TVFIGRQLTIMELGDDIAKGLGQNINKVRIIIGLLVIILTGMSVSVAGPIGFVGLIVP
                     HIVKRYVSKNYLVMIPLTFIIGADLLLLSDVLSRLITYPYESPVGIVTSFVGALYFLF
                     ITIKGVKRI"
     gene            24485..25453
                     /locus_tag="NBH32_000992"
     CDS             24485..25453
                     /locus_tag="NBH32_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HCD1514611.1"
                     /translation="MMTTKIIRRYSLITILLIVSIFISLCVGSVMIHPIDAIKGIFAQ
                     DDFILNEYRIPRTLLGIIIGSSLAISGAIIQAVIRNPLASPDVIGISKGASLAAVIII
                     MIFPTAPLFVLPIGSFLGAFAVSLFLSFLISKYDVKGSKLALIGLAIGAICTAIVQFL
                     LIRNPLEANKALVWLTGSLYGHDMSNIYTILPWFLITIPIIILLSYQLDVLNLGDAVA
                     TALGLKVKTIKMLLLILAVILAGSAISVVGGISFLGLIAPHIARQLIGNKTLHVIIMS
                     GLIGAILLTFGDGLARGIHPPLDIPVGVIIAIIGAPYFLILLRRMK"
BASE COUNT         8893 a         3687 c         4777 g         8276 t
ORIGIN      
        1 gcagctatta tttttttact tgttttatgt ttatttttta atctaacaaa tgacaaacca
       61 ttaaaagtaa catcccaaca tcaaacaaaa aacaattcat caaatgaaaa tcgttataaa
      121 accaagtcat taaacacgca ataattaaaa ttttccactc attttaattt ggaacttcaa
      181 acgtcgaatt ccaaaaacga aactccaaat tccaaaaacg caactccaaa attaaaagca
      241 tctccctgtt actcgggaaa tgctttttac ctactgatta ccctgttatt aatgatttta
      301 caacggaaac catgtcgtca tgtatgacca aagtagcgtc gctatcataa ggtgttcgat
      361 ctttattgat aataattaaa ttgtcgcctt taaaatttga tattaatcct gcggcaggtt
      421 gtacaacgag tgatgaacct agtacaacaa gggtgtcggc atgttcaatt ttatttaatg
      481 cccgtatgat ggtaggttga tctaacattt caccgtataa tacgatgtcc ggtcgaatgg
      541 cgccaccaca attatcacaa tgtttcaaag ttctatcaat gacatctgac ttcgtataag
      601 atttatgaca tgcattacaa taaaaacgat ttaacgtgcc atgtaattca tcaacatgtt
      661 gacttccagc gtctgagtgc aaaccatcga tattttgcgt gatgacacct aaagattgtt
      721 gattacgttc taattttgca atccaatcat gaacgatatt aggcttcgta tcgacaaata
      781 gtaaacgctt atggcagaaa ttgataaaac cttcaggatc atcttctaaa taatcacggc
      841 ttaacaagta ttctggtgaa agcccatctt ttgaaatttc atcaaataag ccacccattg
      901 aacggaaatc tggaacacca cttgcgacag atacaccagc acctgtgaaa aatgtaatac
      961 gattcgaact atctataata tgttttaatg tctctaaatc attttccaaa ttaaacaccc
     1021 catcaaaata atgaatgatt tataacgtct tttgctttta ttatagtata ttcattttac
     1081 gattatcaaa agcgcagttt aaacagttct ttttaaaaat gaattataca tataatcgaa
     1141 cagttaaatt atattagttt ttacgcaatt tttattatag aataagcatg tatattaaaa
     1201 ttgatgtcta aacataaagg ataatgaccc atcgcctaaa taaaggaggc aattttacat
     1261 tgaataaaca tgaacgtttg gacgaaattg ctaaactagt gaataaaaag ggcacgataa
     1321 gaacgaatga aatcgtcgaa ggtttaaatg tgtctgatat gacagttcga agagatttaa
     1381 ttgaattgga aaataaaggg attttaacga agattcatgg tggtgcacgc agtaattcaa
     1441 catttcagta taaagaaata tcacataaag aaaaacatac ccgtcaaatt gcagaaaaac
     1501 gatttatagc tagaaaagct gcatcattaa ttgaagatgg ggatactttg tttttcggac
     1561 caggaacaac agtggaacta ttagcagaag aagtcaatca tcatacgctc acaattatta
     1621 cgaattgttt gccggtgtat aaaattttgt tagaaaaaca aacagcacat tttcgtgtct
     1681 atttaattgg tggtgaaatg cgccatatta cagaagcatt tgtaggtgaa atggcgaatg
     1741 ccatgttgga aaaactaaga tttagcaaga tgttctttag tagtaacgca gtaaataaag
     1801 gtgctgtgat gacatctaca ttagatgaag cctatacgca acaactggca ctcagtaatt
     1861 caattgaaaa atacttgtta atcgatcata cgaaagttgg caaagaagat tttacatcat
     1921 tttgtcagtt aaacgaattg actgcagtgg tcatggatta tgaagatgaa gaaaaggtag
     1981 aaacgattaa aacatatatt gaagtagtcg actaaattaa aaatataact gcattgttaa
     2041 aatatgtgtc tatatgctcg taagtgaatg tgaatttgca ttcatttatg ggctattttt
     2101 tatgcaaatt tatgtaaacg ggaacctcga caaagtttta ctacaatttc agacgaatac
     2161 ctaattaaaa caacaccaca gtatgaatgt tcacttattg tttgaaaata tgtcataaac
     2221 aaacagatta taaacatatt atgttgattt attgtttgtt tatgtttata atttaaacgt
     2281 aatcaaattg aaagcctttt ctcaagattt tcataaccta caaataaaca tgtcatattt
     2341 taagaaaggt atttcaaaat taaagtaaaa aggagtctta ttatggcgat tattattggt
     2401 tcagatgaag ctggcaaacg attaaaagaa gtcatcaaat catacttatt agacaataaa
     2461 tatgatgttg ttgacgtaac agaaggacag gaagttgact ttgttgatgc aactttggct
     2521 gtagcaaaag atgttcaaag tcaagaaggt aacttaggta ttgttatcga tgcatttggt
     2581 gcaggtagct ttatggttgc tacaaaaatc aaaggcatga ttgctgctga agtatcagat
     2641 gaacgctctg gatatatgac tagaggccat aacaattcaa gaatgatcac tatggggtct
     2701 gaaattgttg gagacacatt agcgaaaaat gtcgttaaag gttttgttga aggtaaatac
     2761 gatggtggta gacaccaaat ccgtgtagat atgcttaata aaatgtgcta gggaggaaaa
     2821 attactatga agattgcatt aggatgcgac catattgtta cagatacaaa aatgcgtgta
     2881 tctgaatttt taaaatcaaa aggacatgaa gtcattgacg taggaacata cgatttcaca
     2941 agaacacatt atccaatttt tggtaaaaaa gttggcgaac aagttgttag cggtaatgca
     3001 gacttaggtg tttgtatttg tggaacaggt gttggtatta acaatgctgt aaataaagta
     3061 cctggcgttc gttcagcact agtacgtgat atgacatcag ctttatatgc taaagaagaa
     3121 ttaaatgcga acgttattgg ctttggtgga cgtattatag gtgagttatt aatgtgcgat
     3181 attatcgatg catttattaa tgctgaatat aaaccaactg aagagaacaa aaaattaatc
     3241 gctaaaatta aacatttaga aacaagcaat gcagatcaag ctgatccaca tttctttgat
     3301 gaattcttag aaaaatggga cagaggcgaa taccacgatt aagggacgac acatatgatt
     3361 ttaactttaa cattaaatcc atccgttgat atttcatatc cattaacagc attgaaatta
     3421 gatgatgtta atcgtgttca agaagtaagt aaaacagctg gtggtaaagg tttaaatgta
     3481 acaagagtac ttgcgcaagt aggtgagcca gtccttgcga gtggttttat tggcggtgaa
     3541 ttaggtcaat ttattgctaa aaaattagat catgccggta tcaagcatgc attttataac
     3601 attaagggtg aaacacgaaa ctgcattgca attttacatg aagggcaaca aacagaaatt
     3661 ttagagcaag gaccggagat tgataatcaa gaggctgcag gatttattaa acattttgaa
     3721 caattattag aaaaagttga agcagttgct atttcaggta gcttacctaa aggattaaat
     3781 caagattatt atgcacaaat cattgaacga tgccaaaaca aaggtgtgcc tgtaatatta
     3841 gactgctcgg gtgcaacttt gcaaacagtt ttggaaaatc catacaaacc aacagtcatt
     3901 aaaccaaaca tttctgaact atatcaattg cttaaccagc cacttgatga aagtttagaa
     3961 agtttgaaac aggcagtgtc acaaccctta ttcgaaggga ttgagtggat tattgtgtct
     4021 ttaggtgaac aaggtgcatt cgctaagcac aatcatacat tctatagagt gaatattccg
     4081 ataataagtg tattaaatcc ggttggttca ggtgattcta cggttgctgg aattacttca
     4141 gcaattctca atcatgaaaa cgaccacgat ttactaaaaa aagccaatac tttaggcatg
     4201 ctgaatgcgc aagaagcaca aacaggttat gtcaatttaa ataattatga tgatttattc
     4261 aatcaaattg aagttttaga ggtgtaaaaa atgtcaaaat cgaatcaaaa aatcgcatca
     4321 attgaacaat taagtaacaa cgaaggtatc atttctgcat tagcatttga ccaacgtggt
     4381 gctttaaaac gcatgatggc aaaacatcaa actgaagaac caactgtagc tcaaattgaa
     4441 caattaaaag tattagttgc ggaagaatta acacaatatg catcttcaat tttattagat
     4501 cctgaatacg gtttaccagc ttctgatgca cgtaacaaag attgtggatt attacttgct
     4561 tatgaaaaaa ctggttatga cgtaaatgct aaaggtcgtt tacctgactg tttagttgaa
     4621 tggtctgcaa aacgattaaa agaacaaggc gcaaatgctg ttaaattctt actttactat
     4681 gatgtagatg atacagaaga aatcaatatt caaaagaaag catatatcga aagaatcggt
     4741 tcagaatgtg tagctgaaga tattccattc tttttagaag tattaacata cgatgacaac
     4801 attcctgata acggtagtgt tgaattcgca aaagttaagc ctagaaaggt taacgaagca
     4861 atgaaattat tctcagaacc acgctttaac gttgatgtat tgaaagttga agtaccagta
     4921 aacatgaaat atgtagaagg ctttgctgaa ggtgaagtgg tttatacaaa agaggaagcg
     4981 gcacaacact ttaaagatca agatgcagca actcatttac catacattta cttaagtgct
     5041 ggtgtttctg cggaattatt ccaagaaact ttaaaatttg ctcacgaagc tggtgctaaa
     5101 ttcaacggtg tactttgtgg ccgtgctaca tggtctggtg ctgttcaagt atacattgaa
     5161 caaggtgaag atgctgctcg tgaatggtta cgtacaactg gtttcaaaaa tattgatgac
     5221 ttaaacaaag tattaaaaga tactgcgaca tcttggaaac aaagaaaata agtataaaag
     5281 gggattaatg ctatgaatag agaagaagtc caattattag gttttgaaat tgttgcattt
     5341 gcaggggatg cacgttctaa gtttttagaa gcattaacag cagctcaagc tggagatttt
     5401 gcaaaagcag atgcactgat agaagaagga aacaattgca ttgcggaagc gcatagagca
     5461 caaacaagcc tgttggctaa agaagcgcaa ggtgatgata ttgcatatag tgtgacgatg
     5521 atgcatggtc aagaccattt aatgacaaca attttactga aagatttaat gaagcattta
     5581 ttagaatttt ataaaagagg gtgatgacaa tgatgcaaaa attgatcgca caaattgaaa
     5641 aagggaagcc atttttcgag aaattatcac gtaacatata tttaagagcc atccgtgatg
     5701 gatttatttc tgcaatgcct gtcatcttat tctcaagtat ctttctatta attgcatacg
     5761 tgccaaatat atttggtttt aaatgggata aaggcatgga agccatttta atgaaaccat
     5821 ataactatac gatgggactt gtagcatttt tagtagcagg aacgacagcg aaatctttaa
     5881 cagattcatt taatcgtaag ctagaaagta caaatcaaat taactttatt tctacgatgc
     5941 tagcagcgat gtgtggattc ttgttcttag cttctgatcc agccaaagat ggtggattct
     6001 taagtgcatt tatgggaact aaaggtttat taacagcatt cttatcagca tttgtaactg
     6061 ttattgttta taacttctgt gtgaagcgca atattacaat taaaatgccg aaagaagtac
     6121 cgccgaatat ttcacaagta tttaaggact taattccatt ttcagcggta atcatcattc
     6181 tttatgcatt agatttagtc attcgcaaca gctttaaatc aaatgtagcg gaaggtattt
     6241 taaaattatt cgaaccatta tttacagcag cagatggatg gattggtgtc acaattatct
     6301 ttggtgcctt tgcattattc tggtttgtag gaattcatgg tccatcaata gtagaaccag
     6361 caattgcagc cattacatat gcgaatatcg aagcgaactt caagttgctt caagctggag
     6421 aacacgcaga taaaattatt acatcaggta cacaaatgtt tatcgttact tttgggggta
     6481 cgggtgcgac attggtcgtc ccattcatgt ttatgtggat gacgaaatct aaacgaaaca
     6541 aagcgattgg acgtgcatca gttgtaccaa cattctttgg tgtaaacgaa ccaattttat
     6601 ttggtgcacc actagtattg aatcctgtgt tctttattcc atttgtatta gcaccaattg
     6661 ttaacgtatg gattttcaaa cttttcgttg aagtgttagg aatgaatagt tttagtgtga
     6721 atttaccttg gacaacacca ggtccattgg gcattatcat gggtacaggt tttggtttat
     6781 ggtcattcgt actagctatt actttgattg ttgtagatat cattatttac tacccattcc
     6841 taaaagttta tgatagtgaa attcttgatg aagaagaagg acgtaaagaa agtaattcag
     6901 atttaaaaga aaaagttgca gcaaactttg atacgaaaaa agctgattca attttagcgg
     6961 caagtggtgt atcagacgat gcagctaaag cttcaaatat cacagaacaa acaaatgtct
     7021 tagtactttg tgccggtggt ggtacaagtg gattattagc caacgcactt aataaagcag
     7081 ctgaagaata tcatgtgcca gtgaaagcag cagctggcgg atatggcgca catatggaca
     7141 ttatgaaaga gtaccaatta atcattttag cgccacaagt tgcgtctaac tatgaagata
     7201 ttaagcaaga tacagataga ttaggtatta aattagcgaa gactcaaggt gctgaatata
     7261 tcaaattgac acgagatggc caagcagccc ttgatttcgt tcaacaacaa tttgaaaatt
     7321 aagaatagga gtttttcata tgactaaaac attacctgaa gattttattt ttggtggagc
     7381 aacagcagca tatcaagcag aaggtgcgac aaatacggat ggtaaaggtc gtgtagcatg
     7441 ggatacgtat ttagaagaaa actattggta cactgcagaa ccagcgagtg atttttataa
     7501 cagatatcca gtggatttag aattaagtga aaaattcggt gttaatggta tccgtatttc
     7561 aattgcatgg tctcgtatct tcccaaatgg atatggcgaa gttaacccta aaggtgttga
     7621 gtattaccat aagttatttg cagagtgtca taaacgtcat gtcgaaccat ttgtaacatt
     7681 gcatcatttt gatacaccag aagtgttaca caaagatggc gattttctaa atcgtaaaac
     7741 gattgactac tttgtagatt atgctgaatt ttgttttaaa gaattcccag aagtaaaata
     7801 ttggactaca ttcaatgaaa ttggacctat tggtgatggt caatatttag taggtaaatt
     7861 ccctccaggt attaaatatg attttgaaaa agttttccaa tctcatcaca atatgatggt
     7921 tgctcatgca agagcagtaa aattatttaa agatggcggc tatcaaggag aaattggtgt
     7981 tgtacacgca ttgccaacaa aatatccgtt cgatccatca aatcctgaag atgttcgtgc
     8041 agcagaatta gaagatatca tccataataa attcatctta gatgcaacat atttaggtaa
     8101 gtattctcgt gaaacgatgg aaggtgtgca acatatctta tctgttaatg gcggtaaatt
     8161 aaacattaca gatgaagatt atgccatttt agatgcagcc aaagatttaa acgacttctt
     8221 aggtatcaat tactacatga gtgattggat gagaggttac gatggagaat ctgaaatcac
     8281 gcataatgcg acaggtgata aaggcggttc taaataccaa cttaaaggtg tcggacaacg
     8341 tgaatttgat gtcgacgtac ctcgcacaga ctgggactgg atgatctatc ctcaaggttt
     8401 atatgatcaa atcatgcgtg ttgttaaaga ttatcctaac tatcataaga tttatatcac
     8461 tgaaaatggt ttaggatata aagatgaatt tattgaatct gaaaaaacag ttcatgatga
     8521 tgcacgtatt gattatgtaa gacaacattt aaacgttatt gcagatgcta ttaaagatgg
     8581 tgcaaatgtt aaaggttact ttatttggtc attaatggat gtgttctctt ggtcaaatgg
     8641 ttatgaaaaa cgatatggtt tattctatgt cgattttgag actcaagaac gttatcctaa
     8701 gaaaagtgct tattggtaca aagagttagc cgaaacaaaa gaaattaaat aattatagat
     8761 attaaaaggc ctatcagtcg agtgctggta ggtctttatt tatagaaaga gataaattaa
     8821 acatgcatat acatatttat atattaatca atgggtgaca caccattgaa agctaaagaa
     8881 gcaatcaatt aacaaacgtt aacggacaac tttccaaagt catcttaaaa gttaatgatg
     8941 tattcaaaat caactaaagg acatttttaa tcacatcctt tatacttata taaaagacat
     9001 gaatgggagt taaaagtgtg aagaaaattt ttgtaattat tacgacattg ttagcggtcg
     9061 ctattattat aggctctatt attacggtgg tgtttagtca acgacaggca caaacattta
     9121 aaatacaaca acagcaattt gttaaaaagc caattccaac attgttctta catggatttg
     9181 gtggtagtgc caattctgaa aagtttatgg tgaagcaagc agaaaaaaga ggggtaacta
     9241 aagatatcat cactgcttac gtttcaaagg atggtgcagt gacatttaaa gggaaactaa
     9301 gaaaagatgc agtgaatcct attgtgaaaa ttgaacttga aaataacaga caaggctatt
     9361 tggataagaa tgctgcgtgg tttaaaaatg tactgactaa gttacaatct gaatacaatt
     9421 tcgataaatt caactttgtc ggtcactcaa tggggaactt aacatttgca caatatatga
     9481 tgacatatgg taacgacaag tcactgccac agttaaacaa acaagtgaat attgcaggga
     9541 catttaatgg tgttttaaat atgaatgaag atgtcaatga aattacagtt gataaagatg
     9601 gtaaaccaag tcgaatgaat caaccttatc agcaattacg cgtgctaaaa gatatttata
     9661 aaggtaaagg tatcgaagtg ctaaatattt atggagattt gaaagatggt acacattcag
     9721 acggcagagt gtctaatagt tcttcgaaat cattaaaata tttactggga aatagtccga
     9781 aaagttatcg agaatctaaa tatgaaggtg agcctgcaca acatagtcag cttcatgaga
     9841 atgaaaatgt tgctaatgag ttaatcgact tcttatggaa aaaataaagc aactgctagt
     9901 ttttctgggg caaaaaagct tgtaatatta gagcttttca aaaattatct attgtttgag
     9961 agtttccata aaatgtctaa gtcgtaacat tttaaattta tgattatagc aaatataatg
    10021 tggttgaagt tacatgaatg aatcagtgct taagtcaaaa tataacaact aagaatgtat
    10081 ttgcatacag acatcacgaa gacacaattc aaacttaacg cttaagtaac aaataagtaa
    10141 aagtttcaaa taaagaaagg tgattaatga tgcaaaataa acaaattctt tttaacaaaa
    10201 taccagaagg catgcctcaa gaggatacat tcaaaattga agagattgac acgcctaaac
    10261 ttgagagcga tggtgtactt gtacaaacgc tgtatatttc agtggatcca tatatgagag
    10321 gacgtatgac taaggcagat tcgtatgtac agccttttga aattggtaaa ccgattgtta
    10381 gccatgtcgt tgcaaaagta attgattcga cactcgcaga ttataaaaaa ggagatgtcg
    10441 ttgtaggtat gttgccttgg cgaatcatta atcatgtcca agcggatcaa attactaaag
    10501 ttccaacgac agatgtacca cttgatttat atttaagtgt tttaggaatg ccaggacaaa
    10561 cagcatatca tggtctgctt gatattggac aacctaaagc cggtgatact gttgtggttt
    10621 cagctgcatc tggtgctgta ggttcagttg taggtcaaat tgctaagatt aaaggttgcc
    10681 gtgttgtagg tattgcgggt ggcgacaaaa aagtaaatta tttaacagag acactgggct
    10741 ttgacgctgg tattgattat aagaaagagg attttgcaga agcgttagca caagcagtac
    10801 cagatggtat tgatgtttat tttgaaaatg ttggtggtac agttggcgat gaagtattta
    10861 agcatttaaa tcgttttgca cgtgtaccag tatgtggcgc aatttcgtca tataatcatc
    10921 ctgaagccga tatcggacca cgtattcaag gtacgcttat taagaagcaa gtaatgatga
    10981 gaggcttctt agtaagtgaa ttcgccaatg cttttaaaga agctagtgaa cagctcgcaa
    11041 catgggttca agaaggtaaa attcagtcac aagtaaccat tgaagatggt tttgaaaatg
    11101 cgccacatgc atttaaaaat ttatttactg gcgataactt tggtaaacaa gttattaaag
    11161 taacggaata ataaagtata cgttttaaca aactaacttt gttaattgac caaaatttaa
    11221 taatttttta tctaaagcaa atttgttcag taacatggta gaaataagtc taaaatgtaa
    11281 tacgaaagtc gtctgtaatt atttatatat tcagatgaaa aaggagtcat gatgctgcga
    11341 taaaagcaca catggcttct attttgtata ataagaaata agttcataaa tgaggtgaaa
    11401 cgatatgaaa atgataggat ttgaaaagcc ctttaaattg gaagaaggaa atttatttaa
    11461 agtatacgaa caaagaaagc caacgccaga aaacgatgat atcttagtca aagtaaatag
    11521 cattagtgta aatcccgtag atacaaagca acgacaaatg gaagtcactc aagcacctcg
    11581 tgtacttggg tttgatgcta ttggtactgt tgaagctatc ggaccagacg ttacattatt
    11641 tagtccgggt gacgttgtat tttatgctgg atcgccaaat agacaaggat caaatgcgac
    11701 atatcaattg gtttcagaag ctatagttgc aaaagcgcca cacaacataa gcgctaatga
    11761 agcggttagt ctaccgctaa ctggtattac ggcatatgaa acgttttttg atacatttaa
    11821 aatttcacat aatccttctg aaaatgtagg taaatccgtg ttaatcatta atggcgctgg
    11881 gggtgtagga agtattgcca ctcaaattgc taaacggtat ggtttaactg tcattacaac
    11941 agcatcacga caagaaacaa cagagtggtg tgaaaagatg ggagcagata tcgtattgaa
    12001 tcataaagag gatttagtgc gtcaatttaa agaaaaagaa attccattag tagattatat
    12061 attttgtacc tataatacag acttgtatta caatactatg attgaactga taaaaccatt
    12121 agggcatatt acaactatcg tagcttttaa tgaagatcaa gatttaaatg cattaaaatt
    12181 aaaaagtata acatttacac atgaatttat gtttgcgcgt ccaattcatc gaacgcctga
    12241 tatgattaaa caacatgagt atttagaaga tattacgaaa aatatagaat caggacatta
    12301 ccaaccaaca acgacacaag tttttgaagg tttatcacct gaaaacttat atcaagcaca
    12361 tctattgttg gaaaaacagt caatgattgg gaaattagta attaatatat agtttagtaa
    12421 gtaaagtcgc cttatgcaga aaatactgta tggggcgact tatttttgtg tctagcgact
    12481 attgattcta aagggaaata tgacaactta gaaggtatca attcaataat gcattagaaa
    12541 ggcattactt aaatacaata aaatactttg ttgttcaatg ataaataatt tgagtatgtt
    12601 ttatatatca tttttaaaag atgaagcatt attaattgtg tctatataaa attacaaatg
    12661 attaaaatgt tgcagttgtg ggtatgaatt taccattaca cagaaagggt ttgtatttat
    12721 gaaaaataaa tcagtaagtc tagtgttttg ggtatcgtta gtgatttgta cgatatttgt
    12781 tgcttttggt gcaatatttc cgaaacagtt agaaaaactc acgcaaaaca ttactacatt
    12841 tattgcgcta catttttctt ggtattattt attgctcgtt ttagttattt tatttgtctg
    12901 tgtgtatatt ttattttcaa gatacgcaaa cattacatta ggtgaagaag gcgaagatcc
    12961 tgaattctca ttgccgtctt ggtttgctat gttatttagt gctggtatgg gaataggact
    13021 tgtgttctgg acaacagcag aaccaatcag tcatgccttc aaattaacgc ctattcataa
    13081 agctggcacg caatctgcaa ttaatgatgc aatgcagttt tcatttttcc attgggggat
    13141 tcatgcatgg gcagtctatg gtattgtcgc gcttgtgttt gcatatttta gctttcataa
    13201 aggatatcct ggcttagtca gcgctacatt gacgccttta ttaggtgaaa aggcaatgcg
    13261 tggaccttta ggtggtgcca ttgatgtatt ggcagtcata gctacagtaa caggcgttgc
    13321 tgcaacatta ggtttcggtg cattgcaaat aaacgaaggt ttacattttt tatttaatgt
    13381 accgagtaac ttcacgatgc aagtaatctt gattgtcatt acaaccatat tatttacttg
    13441 gtcagcatgg tcaggtatcg ataaaggtat caaaacgttg agtaatatca acatgttact
    13501 tgcttttgta gtacttattg gtctttttat tgttgggcca acgctataca ttttaaatac
    13561 atttacgaat ggtctaggta attatattgc aaatttcttt agtatgagtt tacgcattcc
    13621 gtcaggcggt caaaaattcc aatggttaca aaattggaca atcttttact gggcatggtg
    13681 gatatcttgg gcaccatttg taggtatatt tattgcacgt gtgtcaaaag gacgtacgat
    13741 taaagaattt attctaggtg tactttttgt cccggcatta gtttgcttta tattctttgc
    13801 ggtattcggc gcgtcagcaa tttacttaca agataatcat attgccgata ttgctaaagc
    13861 tgcgacagaa acagcaacct ttgcaacgct acaacattac ccactaggtt ttgtattaag
    13921 cttaattact ttacttgtta ttatgatttt ctttgttaca tcagcggatt cggcaactta
    13981 tgttttaggt atgttgagtt caagaggtga tattaatccg aagtcatttg tcaaagtgag
    14041 ttggggtatc attatggcat tatttgcaat tatcatgatc tacacaggtg gtactcaagc
    14101 gattcaaaat ttattaatca ttgctgcgtt accattttcc gtggtcatta tagcgatgat
    14161 atggtcgctt ttaaaatctt tgagtgagga gaaaccgaga aatagtaata aggttttaat
    14221 taaacatcgt gacccagata ttttagaata tcgttcttca aaacatattg acgaaaatta
    14281 attaataatg aggttttatt tacaaaaact agggaatata ttactaaatg gtacttcatc
    14341 ttatatatta aaaataaatt tatagttatt aatttattat gtttaagaaa aaatctgatg
    14401 ggtacatatt taatatgttt ttaaattaac tttaactaag gaggtgtcgt taggggtgaa
    14461 acgacttaaa aactttatcc ttggcttatt aatagtagta atcgtggggt tcctgttatt
    14521 catgtatatc caagatggac gtataactga atatcaagac tacttccttc aatttgaatg
    14581 gttccaacca ttacttattt cacttgctgc actattgata ttaataggtc ttatattagt
    14641 atttagtatc ttcaaaccta cgcatcgaaa acctggatta tacaaagact tcgatgatgg
    14701 tcatgtttac gtatctcgta aagctgttga aaagacggca ttcgatacgg ttgcaaaata
    14761 tgatcaagta agacaaccaa atgtagttgc aaaactatat aacaaaaaaa ataaatctta
    14821 tatcgacatt aaaactgact ttttcgtacc aaataatgtt caagttcaat ctttaactga
    14881 agcaattcgt tcagatatta aaaagaatgt agaatatttc acagaaatgc cagtacgtaa
    14941 gttagaagtt aatgtgagag atcagaaaac ttctggtcca cgagtgttgt aagggaggta
    15001 tatcatggct aacaatcata accaaaacgg acaagactct acacaacaag tgattaattt
    15061 tttaaaagta tttaaatgga gaatcgttgg tttcttagcg tttctattaa tcgcgatatt
    15121 attcttaacg ttaggttttt ggaaaacaat acttatcatc gttttatgtt taattggtgt
    15181 aggtattggg tatatgaaag accgtaagca agattttatg aattttttaa atcgatggag
    15241 ttaattcatc aatttagaat ttaattaaaa aaatataaaa aacaataagt taaaggagaa
    15301 tctattatga ctgtagataa caataaagca aaacaagcat acgacaatca aactggtgtt
    15361 aacgaaaaag aaagagaaga gcgtcaaaaa caacaagaac aaaatcaaga acctcaattc
    15421 aaaaataaat taactttctc tgatgaagtt gttgaaaaaa ttgctggtat cgctgcacgt
    15481 gaagttaaag gtatcttaga catgaaaggt ggcttaactg atacattcac taatgcattc
    15541 tcaagtggca acaacgttac acaaggtgta tctgttgaag ttggtgaaaa acaagctgct
    15601 gtagacttaa aagtaatttt agaatatggt gaatcagcac ctaaaatttt ccgtaaagta
    15661 actgaattag taaaagaaca agttaaatat attactggtt tagatgttgt tgaagttaac
    15721 atgcaagttg acgatgtaat gactcaaaaa gaatggaaac aaaaacacga aaaaaataat
    15781 gaaaacaata accaagaaag acaaggttta caataagtta ttcgataaaa aagcacggca
    15841 caccatttta gtgccgagct tttttgtgat ttttaataat ttgaatatga gaaatgtatc
    15901 tttctctcat ttgtttagat tgtatttaat aagttaatgt aatccttgag ctcacgatta
    15961 ataaaattct ataaccttaa ttattttctc gatacaaagg gttattaact ttaatataag
    16021 tataatgatg tgcctcatct tcaagacgca ttgttgtaac acacttataa tctataaatg
    16081 gcgcgaacat ggtatcttta atttcattta tgcgattctc atttacttga ttagatttgt
    16141 gtgtcgaaag cacaagttga tcaaagatgt tatctagtac atcacgaacg atataccaca
    16201 tatgtctttc caagtttgaa tcatttgatg ctttagagat tgtaagaatt aattcgccta
    16261 aatggttttg aacggtagaa taaaaggctt tgttaaacac agacgttttt gaatcagtaa
    16321 gaattcttga tttttcatgg aaatgagatg tactgtatcc catttcattt agttgtgctt
    16381 tatcaattct taaaccttca aagtctctaa tatacatcgt gtccaataaa ccatctttcc
    16441 gaaatgttgc aatagcattt tgcaagtgag cctctaatgc aatgccatat ttagtaacta
    16501 gtgggattac gagaccaagc aatgctttac tataagtttc aatccacgat ttcgccgatg
    16561 attcaaaatc agacaatgat gcagctgatt gataacgttt aatcaatgtc acgacaggtg
    16621 attcattgtt aaatgggtag gttgcaacta aacttgaagg aatcattggt gtgacttctt
    16681 gtggaatcat ttggtatatg ttttttctaa ataatgtacc taattgttca cttcgttcag
    16741 tttgatagtc tgcttcgtct tgctcattat aaaaatgaat accagcaact tcatcaataa
    16801 ttgttgatgc ataggactta aatatgacat ctttctccaa aatatcattt aaaatacgtg
    16861 tcattagtgg accattgtgc gttgtttgtt ctgataatgt acgaatttca cctgtaatat
    16921 gaacgtttgt cgacaatttg atgtgtggcg acatagctgg gtatttagga actaatgttc
    16981 tgaaagataa accagcataa taatccaacg tatgttttgc ttcaatgatt aattctttat
    17041 ctacttctgc ttgataatca gaatgtaata cgtcatctaa ttgccatgga tgtacaatca
    17101 taatgtgata atcattaaag ttaaactttg gcgtaaattc attttctaat tgtttaatta
    17161 agtccggaaa tagttgatga acagttgtat cataatcttt agacagtgac atagtacggc
    17221 ttaatttact gtgaatcagt actattttca acttaatagg ttgattgaat tctgaagaat
    17281 ataggaatgt ttgtaatgca tttaaacctt tacgtaattt agccccagga tgtagcggat
    17341 gaccttcaat aacggcttgc tctgaacgca agtaactatc ttcgctattt tcgataatat
    17401 taaataaagg tgtagaatca tgttgcattg acagtgcttg atagctaatt gcaaatgtca
    17461 tattagttgc actgtttatt aaatcttgct gaaattgatc actagcagca ttttttaaat
    17521 ctggtgcttc aattaaaata cactcaagaa tttcatttgg atggtgtact cgtgtaatcg
    17581 tatttgtaat gtcatcttta atgtagaaag ggccttcaac atcaattcga tcaaaggcat
    17641 gttctccagt gataggagca tataatgttt gcttagcttg tgggaactgg atttctagta
    17701 tatgagtcgt tgagatatct aacataatca aatcacgact caatattttc ttactttgag
    17761 tgcgcgcttt aactaagttt tcgcgatgca ttgatgtgac taatctctga gtgactttat
    17821 ctcttccttg taaaatcatc tctttaaaaa tattagccca atcgttatta tgttgttgta
    17881 aaaataaata tgtttcttgt tcttctttgt taaattttaa tgtctgttct ttaaaaatta
    17941 agttcaagtt cataattcac ctctatgaaa tattttacaa aagcaagata gatttgtata
    18001 atccatatta atgataatga ttcttattat caacagaatg cgggtgtaag ttttatgaca
    18061 aaatattttt ttagcagttc ttttctacta tttctaggta attggattgg acaaataggg
    18121 ctaaattggt ttgtacttac cacttatcat aacgcagttt atctggggat tgtcaatttt
    18181 tgcagacttg taccaatatt attactaagt gtgtgggcag gggcaattgc cgataaatat
    18241 gataaagggc gattgctgag aattacaatt tcatcatcat ttttagtaac tgcaatttta
    18301 tgtgtgctta cgtatagttt cactgcaatt ccaattagca tcattattat atatgcgaca
    18361 ttaagaggga ttttaagtgc ggttgaaaca cctttaagac aagcaatctt accagattta
    18421 tcagataaaa tatctactac acaagctgta tcatttcatt cattcatcat taatatttgt
    18481 cgttcaatag ggcctgccat tgctggtgtc atattagcag tctatcatgc gccaacaaca
    18541 tttcttgcac aggcaatttg ttattttatc gcagttttat tatgtttacc attacatttt
    18601 aaagtaacta aaatgcctga agatgcaaca agatatatgc ctttaaaagt cattatagat
    18661 tacttcaaat tacatatgga aggtcgacaa atatttataa catcattatt gattatggcg
    18721 acaggttttt catatacgac acttttacca gttttgacaa acaaagtatt tccagggaaa
    18781 tctgaaatat ttggtatcgc tatgacgatg tgtgccattg gtggtattat tgcaacgcta
    18841 gttttaccta aagtacttaa atatattggt atggtaaata tgtattattt aagttcattt
    18901 ttatttggca ttgctttgtt aggtgtggta tttcacaata ttgtcatcat gttcatttgt
    18961 attacattga ttgggttatt tagtcaatgg gcacgtacga caaatcgcgt ttattttcaa
    19021 aataatgtta aagattatga acgtggtaaa gtactgagta ttattatgat ggatagaggt
    19081 atgattccat tgggaagtct gttaatgagt atatgtgcag atgtgtttgg cattgttaga
    19141 actttttcaa taatgggaat aagtactata tgcattacaa tggtattcta ttttataaat
    19201 agaaagttga agttaaagtt ggaggaaagt aatcatggta tatcttgaat gggcaaaggc
    19261 agatagaaat attcaatatc gtgtaattaa cgccattatt aaagaacgta tttaccccga
    19321 gcaaacattt atttcgcaaa aaggatcttt aattgaaatt cagtatcata tgcatgtgtt
    19381 gactattgaa gttgttagaa aaagtgcatt agaacgctat gagtttacag gggatattac
    19441 ttatttaaat aaaggtgaaa cgtcattaat tataacttta gaaggtttgt tagatgtgtt
    19501 gaatcatgac tttgatatcc ctatttcaga gcgactacgc gaagagttaa tacacagtcg
    19561 agatagttta gttgaaacat ataagcaaat gtctcataga caaacattaa taagtcaaag
    19621 ttttaaattt tcaagattac cacaagatat taactttttt tcatggttac aacatgtaaa
    19681 agatagtgat aagacagatg atttaactta ttctgagagt ttggtaccag aggggcatcc
    19741 aacacaccct ttaaccaaaa cgaaattgcc cttaactatg gaagaagtac gagcatatgc
    19801 acctgagttt gaaaaagaaa tccctttgca aattatgatg attgaaaaag accatgttgt
    19861 gtgcacagct atggatggta atgatcaatt tattattgat gaaataattc ccgaatacta
    19921 caatcagatt cgtgtgtttt tgaagagttt aggtttgaaa aatgaagact atagagcgat
    19981 tttagtacat ccttggcaat atgatcatac gatagggaaa tattttgaag catggattgc
    20041 taaaaaaata ttaattccaa cgccgtttac aatactttca aaagcaactt tatcatttag
    20101 gacgatgtct ttaattgata aaccatacca tgttaagttg cccgtcgatg tacaagcaac
    20161 aagtgccgtt agaacagtct caactgtgac tactgtagat ggaccaaagt taagttatgc
    20221 tttacaaaac atgttgaatc aatatccagg atttaaagtt gctatggaac cgttcggtga
    20281 atatgcaaat gttgataaag atatggcacg tcagcttgct tgtattataa gacaaaaacc
    20341 tgaaattgat ggaaaaggtg caacagttgt tagtgcaagt ctagttaata aaaatccaat
    20401 agatcaaaaa gttatcgtgg atagttactt agagtggtta aatcaaggaa ttactaaaga
    20461 aagtattacg acatttattg aacgatactc tcaagcatta atcccgcctt taattgcttt
    20521 tattcaaaat tatggaattg cattagaagc acacatgcaa aatacagtag tgaacttggg
    20581 gccacatttt gacattcaat ttttagtgag agatttaggt ggttctagaa ttgatttaga
    20641 aacattacaa catcgtgtat cagatattaa aattacaaat gatagtttaa tagctgattc
    20701 tatagatgca gtgattgcaa aattccaaca tgctgttatt caaaatcaaa tggcagaatt
    20761 aatccatcat tttaatcagt atgattgtgt tgaagaaacc gaattattta acatagtaca
    20821 gcaagtagta gcgcatgcca ttaacccaac actaccacat gcaaatgagt taaaagatat
    20881 tttgtttgga ccaacaatta ctgtcaaagc gttgttaaat atgagaatgg aaaataaagt
    20941 aaagcaatat ttaaatattg agttagataa tccgataaaa aaagaggtgt agtactacat
    21001 ggcacacgtt aacataaata tatcgaagat taaatataac gccaaagtac ttcaaacagt
    21061 ttttcaaagt aaaaatatgc aattcacacc agtaattaag tgcatagctg gtgaccgtac
    21121 aattgtagaa agcttaaaag cgttaggtat caatcatgtt gcagaatcca gattggataa
    21181 cataattagt attgcagatc aggatttaac atatacgtta ttgcgtacgc ctgccaaaaa
    21241 agagatttca gatatgatag aaaaagttga tatgagtatc caaacagaat tatctaccat
    21301 acatcaaata aatgaagtag cagaagtttt aggaaaaaaa cataagattt tattaatgat
    21361 agattggaaa gatggccgtg aaggtgttct gacttatgat gttttagatt atattaaaga
    21421 gattattcac ttgaaaaata tccattttgt aggtttagca ttcaatttta tgtgctttaa
    21481 atctgatgcg ccaagtgatg atgatatctt tatgattaat cgattcgtct cagctgttga
    21541 aagagaaata ggatatcgat tgaaaattat ttcaggtggt aattcgagca tgttaccaca
    21601 acttttatat aatgatttgg gtaaaataaa tgaattacga ataggtgaaa cgttgtttag
    21661 aggtgttgat acaacgacaa atcaagcgat agctatgtta tatcaagatg cgattacatt
    21721 ggaggctgag attttagaaa ttaaacctcg agttaataca caaacgcatg aatcattctt
    21781 acaagctatt gtagatattg gctatttaga cactaaagtc gataatattt cacctatgga
    21841 tcagcatatc aatattttag gcgcttcgag tgatcattta atgcttgatt taaatggtca
    21901 aggacactat caagttggag atcatatatc tttttcatta aattatgaag cactttctca
    21961 ttcgatgtat atgaaaaatt tacataaggt atatattgat gattcaaaga ttgatacgct
    22021 acttcaaaat tttgatgtga aatctcccgc tatggtaaac caatattaat atatttatca
    22081 ataagtctaa gttgacaagt tatactacat atcgtttaca cggttgtatc ataattgttc
    22141 aacttagatt ttttgtattt gttgatttat caaataaagt gcaacagttc gtcacataaa
    22201 attgcaacag ataatatcag ctgaattaca gggataacgg tcatgctaaa tggtgtcaat
    22261 tgcattaatg caaagatgat atagccatga tacattatca gtattttgtc taaggaaatg
    22321 tgctaattgt agtcataatt attagaggaa atatatatag tcatacattt tagtaatata
    22381 aaaagattga acgctacttg acaatgataa ttgttatcaa taaaataata aatgaagtta
    22441 tacatattaa ggagtggaac gatgagaggt ctaaaaactt ttagtatatt gggattaata
    22501 gttgccttat ttttagttgc agcttgtggt aatacggata attcaagtaa aaaagaatca
    22561 tcaactaaag atactatttc ggtaaaagat gaaaatggta cagtaaaagt acctaaagat
    22621 gcaaaacgta tcgttgtatt agagtactca tttgcagatg cattagcagc attagacgtt
    22681 aaaccagttg gtattgctga tgatggtaag aaaaaacgta tcattaaacc agttagagaa
    22741 aaaattgggg attatacttc tgtaggtaca cgtaaacagc caaacttaga ggaaattagt
    22801 aaattaaaac cggatttaat tatcgctgat agcagtagac ataaaggtat taataaagaa
    22861 ttaaacaaaa ttgcaccaac attatcatta aagagttttg atggtgacta caagcaaaat
    22921 atcgatgctt tcaagacaat tgcgaaagct ttagataaag aaaaagaagg cgaaaaacgt
    22981 cttgctgagc atgataaatt aatcaataag tataaagatg aaattaaatt tgatagaaat
    23041 caaaaagtgc ttccagcagt tgttgctaaa gctggtttat tagcacatcc aaactattca
    23101 tatgttggac aatttttaaa cgaattagga tttaaaaatg cattaagtga cgatgtaaca
    23161 aaaggtttaa gtaaatattt gaaaggacct tacttacaat tagacactga acatttagct
    23221 gatttaaatc cagagcgtat gatcattatg actgatcacg ctaaaaaaga ttctgctgaa
    23281 ttcaagaaat tacaagaaga tgcaacatgg aaaaaattga atgcagttaa aaataatcgc
    23341 gtggatattg ttgaccgtga tgtttgggca agatctcgtg gcttaatttc ttctgaagaa
    23401 atggctaaag aacttgttga attatcaaaa aaagaacaaa agtaaggtgg aagtaaatgg
    23461 ctataaaaga aataagtagc caatctgcca tagatcataa aagaaaaaga cgcacaacac
    23521 tcacgtatat agtgagtttg tgctttcttt ttatttgtat atatttaaat atggcgattg
    23581 gttcttcgaa aattaatttt agcgatatcg ttcactatgt tactggtcat acagatacga
    23641 aagcaacgtt tttattgcac aatgtacgta tgccaaggat gattgcaggg ttatttattg
    23701 gcggtgcatt agcggtatct ggtttgttaa tgcaagcaat gacacgcaat ccgctagcat
    23761 cacctaaaat ttttggtgtt agttctggcg cgtcatttgt cattgtatta gtaacgatta
    23821 ttattccatc attagaatat tatgcattat atttgggtgt gataggtgca tttataggtg
    23881 gtttaactgt ttatacactt tcaggtgcta cgaaaggtat gacacccatt aaattagcat
    23941 tggctggtat ggcgatccat ttgtttttta gtagtatgac tgaaggtatt attattttaa
    24001 atgagaattc taatgaacaa gtgatgtttt ggcttgttgg ttcattatcc agtatgaagt
    24061 gggatgagat tttaaccatt ctaccatggc ttattggtgc attaattgca acagtcttta
    24121 ttgggcgtca gttaacgata atggaacttg gcgatgacat tgctaaggga ttgggtcaaa
    24181 atataaataa agttagaatt atcattggac tattagtcat tattcttaca ggtatgtcgg
    24241 tgtcagtggc aggaccaatt ggatttgttg gcttaatcgt accgcatata gtcaaacgct
    24301 atgtgagtaa gaattattta gtcatgattc cattaacatt tattataggt gcagatttgt
    24361 tactactatc agatgtatta agtcgtttaa ttacatatcc ttatgaatca ccagtaggga
    24421 ttgttacatc attcgtaggt gcactttact tcttatttat aacgattaaa ggggtgaaaa
    24481 gaatatgatg acaacgaaaa ttatacgtcg ttatagttta atcacaatcc ttttaattgt
    24541 gagtatattc ataagtttat gtgtagggtc tgtcatgatt catccgattg atgctattaa
    24601 gggaatcttt gcacaagatg attttatttt aaatgagtat cgtataccta gaacattact
    24661 tggtattatt atcggtagta gcttggctat atcaggtgca attatacaag ctgtaataag
    24721 aaacccgtta gcctcacctg atgttatagg tatttctaaa ggtgccagtt tagctgctgt
    24781 gattattatt atgatctttc caacagcacc attgtttgta ttacctatag gttcattctt
    24841 aggtgccttt gcagtaagtt tatttttatc atttttaatt tcaaaatatg atgttaaagg
    24901 ttcaaagctt gccttaattg gtttagcaat aggcgcaata tgtacggcga ttgtgcagtt
    24961 cttattaatc agaaatccac tggaagcaaa caaagcgctt gtttggttga caggtagcct
    25021 atatggacat gatatgtcga atatttatac tatattacct tggtttttaa taacgatacc
    25081 aatcatcatt ttattgagtt atcaattaga tgtattgaat ctaggtgatg ctgttgcaac
    25141 cgcactcggt ttaaaagtta aaactattaa aatgctattg ttaattttag cagtaatatt
    25201 agcaggatcc gcaatttcag ttgtcggtgg tattagcttt ttaggattaa ttgcacctca
    25261 tattgcgaga caattaatag gaaataaaac attacacgtt attatcatgt cggggttaat
    25321 tggtgctata ttgttaacat ttggagatgg tttagcccgt ggcattcatc cgccactgga
    25381 tattcctgta ggggttatca ttgcaattat cggtgcacca tacttcttaa tattattacg
    25441 aagaatgaaa taactaacat atgattagag tttaaaaaag gttaagtacg aaatcagtaa
    25501 ttgtacttaa ccttttttga attcatcttg aaaaattaca agaaatagcg tatatttaat
    25561 aactgtaatt attcattatt ttgcatacat aaacatttta aaagatagaa tgataaggaa
    25621 tatgtttgtt aac
//
LOCUS       DAHHOJ010000028        25444 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.028, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000028 DAHHOJ010000000
VERSION     DAHHOJ010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25444
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.028"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(49..1560)
                     /locus_tag="NBH32_000993"
     CDS             complement(49..1560)
                     /locus_tag="NBH32_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1514612.1"
                     /translation="MEKNENINVEILTTSDMHSHFLNGDYGSNIYRAGTYVNQVRAQN
                     HRVILLDSGGSLAGSLAAYYYAIVAPYKRHPMIKLMNRMHYDASGVSPSEFKFGLSFL
                     TRSIALARFPWLSANIEYNVTKEPYFSTPYCIKHFGDLKIAIVGVTADGLMENEYSEM
                     EQDVSIEKTLVASKRWIRYIHEVEEPDFLIVIYHGGLNKISNSTKNKKASSNEAEKLM
                     EELGVIDLMITAHQHQTIVGQDHETYYVQAGQDAKELVHLSINFKKRTTTYDVESIDS
                     KVIDLNEYEEDQELLDLTFYDRKAVAYWSQEIISDKGLMLSVNGLQDLVCQTHPFSQL
                     LHDAIHLAFDNDITCVHVPMNGEKGLSGQIRNEDLYHAYPYPDKPMDMTISGQNIKDI
                     LEYSYSHLDFVNEQLSLTIIDETLCTMWQGFNYEIDMNQEPGQRVMLDQIDLTKSYRV
                     TMTDYCYRNYKNYLKNAIIHESYDETMSTLIAEKLRDPNYRISCSDNFVVKNR"
     gene            complement(1699..3057)
                     /locus_tag="NBH32_000994"
     CDS             complement(1699..3057)
                     /locus_tag="NBH32_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1514613.1"
                     /translation="MSIFSQFLKRSSPQQGIVLYYIVAIVIAFLLLNLPYVHKPGVEV
                     NPIDTLFVAVSGISVTGLSPISIVDTYSTFGQLIILVILNIGGIGVMAIGTMLWVVLG
                     KHIGIRERQLIMLDNNKNTMSGTVKLIIDIVKSIFVIELVGAMLLAFYFYRDNPDLKY
                     AIMQGVFVSISATTNGGLDITGKSLIPYAHDYFVQAIVIFLIILGSIGFPVLLEVKAY
                     IQNRVTNFRFSLFTKITTSTYLFLFIVGVLAILLFEHNHAFKGLSWHQSLFYSLFQSA
                     TTRSAGLQTIDVTTLSDPTNIIMGILMFIGSSPSSVGGGIRTTTFAILILFLINFSNN
                     ADKTSIKVYNREVHIMDIQRSFAVFTMATILTFLGMLIISATENGKLTFLQVFFEVMS
                     AFGTCGLSLGVTSDISDISKVVLMVLMFIGRVGLISFIIMIAGRREPDKFHYPKERIQ
                     IG"
     gene            complement(3074..5398)
                     /locus_tag="NBH32_000995"
     CDS             complement(3074..5398)
                     /locus_tag="NBH32_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HCD1514614.1"
                     /translation="MEWTLVDIGKKHVIPKSQYRRKRREFFHNEDREENLKQHQDKQN
                     IDHTTSKKADKQIHKDSIDKHERFKNSLSSHLEQRNRDVNENKTEESKSNQDSKSAYS
                     RDHYLTDDVFKKQNSLDSVDQDTEKSKYYEQNSDATLSTKSTDKIESTDMRKLSSDKN
                     KVGHEEQHVLSKPSEHDKETRIDFESSRTDSDSSMQTEEIKKDSSDENKSSNLKSEVI
                     SDKSNTVPKLSESDDEVNNQKPLTLPEEQKLKRQQSQNEQTKTYTYGDSEQNDKSNHE
                     NDLSHHTPSISDDKDNVMRENHIVDDNPDNDINTPSLSKTDDDRKLDEKIHVEDKHKQ
                     NADSSETVGYQSQSSASHRSTEKRNISINDHDKLNGQKTNTKTSANNNQKKATSNLNK
                     GRATNNNYSDILKKFWMMYWPKLVILMGIIILIVILNAIFNNVNKNDRMNDNNDTDAQ
                     KYTTTMKNANNTVKSVVTVENETSKDSSLPKDKASQDEVGSGVVYKKSGDTLYIVTNA
                     HVVGDKENQKITFSNNKSVVGKVLGKDKWSDLAVVKATSSDSSVKEIAIGDSNNLVLG
                     EPILVVGNPLGVDFKGTVTEGIVSGLNRNVPIDFDKDNKYDMLMKAFQIDASVNPGNS
                     GGAVVNREGKLIGVVAAKISMPNVENMSFAIPVNEVQKIVKGLETKGKIDYPDVGVKM
                     KNIASLNSFERQAVKLPGKVKNGVVVDQVDNNGLADQSGLKKGDVITELDGKLLEDDL
                     RFRQIIFSHKDDLKSITAKIYRDGKEKEINIKLK"
     gene            complement(5617..6420)
                     /locus_tag="NBH32_000996"
     CDS             complement(5617..6420)
                     /locus_tag="NBH32_000996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCD1514615.1"
                     /translation="MLMDPSLILPYLWVLVVLVFLEGLLAADNAIVMAVMVKHLPPEQ
                     RKKALFYGLLGAFVFRFLALFLISIIANFWFIQAAGAVYLIYMSIKNLWQFFKHPEIE
                     SPEAGDDHHYDESGEEIKASNKSFWGTVLKIEFADIAFAIDSMLAALAIAVTLPKVGI
                     HFGGMDLGQFVVMFLGGMIGVILMRYAATWFVELLNKYPGLEGAAFAIVGWVGVKLVV
                     MVLAHPDIAVLPEHFPHGVLWQSIFWTVLIGLVIIGWLGSVVKNKKSHK"
     gene            complement(6722..8284)
                     /locus_tag="NBH32_000997"
     CDS             complement(6722..8284)
                     /locus_tag="NBH32_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCD1514616.1"
                     /translation="MNLKQEVESRKTFAIISHPDAGKTTLTEKLLYFSGAIREAGTVK
                     GKKTGKFATSDWMKVEQERGISVTSSVMQFDYDDYKINILDTPGHEDFSEDTYRTLMA
                     VDSAVMVIDCAKGIEPQTLKLFKVCKMRGIPIFTFINKLDRVGKEPFELLDEIEETLN
                     IETYPMNWPIGMGQSFFGIIDRKSKTIEPFRDEENILHLNDDFELEEDHAITNDSAFE
                     QAIEELMLVEEAGEAFDNDALLSGDLTPVFFGSALANFGVQNFLNAYVDFAPMPNARQ
                     TKEDVEVSPFDDSFSGFIFKIQANMDPKHRDRIAFMRVVSGAFERGMDVTLQRTNKKQ
                     KITRSTSFMADDKETVNHAVAGDIIGLYDTGNYQIGDTLVGGKQTYSFQDLPQFTPEI
                     FMKVSAKNVMKQKHFHKGIEQLVQEGAIQYYKTLHTNQIILGAVGQLQFEVFEHRMKN
                     EYNVDVVMEPVGRKIARWIENEDQITDKMNTSRSILVKDRYDDLVFLFENEFATRWFE
                     EKFPEIKLYSLL"
     gene            complement(8284..8544)
                     /locus_tag="NBH32_000998"
     CDS             complement(8284..8544)
                     /locus_tag="NBH32_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueH family protein"
                     /protein_id="HCD1514617.1"
                     /translation="MALLINKRFIDEGKTIDVYLFEALNNQIIIAIPDWFWSYQMAMT
                     LDEETCFEAILMQLFVFKEEEEAESIASQLTDWIETYKKEKD"
     gene            complement(8525..10009)
                     /locus_tag="NBH32_000999"
     CDS             complement(8525..10009)
                     /locus_tag="NBH32_000999"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HCD1514618.1"
                     /translation="MDASTLFKKVKVKRVLGSLEQQIDDITTDSRTAREGSIFVASVG
                     YTVDSHKFCQNVADQGCKLVVVNKEQSLPANVTQVVVPDTLRVASILAHTLYDYPSHQ
                     LVTFGVTGTNGKTSIATMIHLIQRKLQKNSAYLGTNGFQINETKTKGANTTPETVSLT
                     KKIKEAVDAGAESMTLEVSSHGLVLGRLRGVEFDVAIFSNLTQDHLDFHGTMEAYGHA
                     KSLLFSQLGEDLSKEKYVVLNNDDSFSEYLRTVTPYEVFSYGIDEEAQFMAKNIQESL
                     QGVSFDFVTPFGTYPVKSPYVGKFNISNIMAAMIAVWSKGTSLETIIKAVENLEPVEG
                     RLEVLDPSLPIDLIIDYAHTADGMNKLIDAVQPFVKQKLIFLVGMAGERDLTKTPEMG
                     RVACRADYVIFTPDNPANDDPKMLTAELAKGATHQNYIEFDDRAEGIKHAIDIAEPGD
                     TVVLASKGREPYQIMPGHIKVPHRDDLIGLEAAYKKFGGGPVDQ"
     gene            10442..11617
                     /locus_tag="NBH32_001000"
     CDS             10442..11617
                     /locus_tag="NBH32_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diglucosyl diacylglycerol synthase"
                     /protein_id="HCD1514619.1"
                     /translation="MVTQNKKILIITGSFGNGHMQVTQSIVNQLNDMNLDHLGVIEHD
                     LFMEAHPILTSICKKWYINSFKYFRNMYKGFYYSRPDKLDKCFYKYYGLNKLINLLIK
                     EKPDLILLTFPTPVMSVLTEQFNINIPVATVMTDYRLHKNWITPYSTRYYVATKETKQ
                     DFIDVGIDPSTVKVTGIPIDNKFETPINQKQWLIDNNLDPDKQTILMSAGAFGVSKGF
                     DTMITDILAKSANAQVVMICGKSKELKRSLIAKFKSNENVLILGYTKHMNEWMASSQL
                     MITKPGGITITEGFARCIPMIFLNPAPGQELENALYFEEKGFGKIADTPEEAIKIVAS
                     LTNGNEQLTNMISTMEQDKIKYATQTICRDLLDLIGHSSQPQEIYGKVPLYARFFVK"
     gene            11595..12785
                     /locus_tag="NBH32_001001"
     CDS             11595..12785
                     /locus_tag="NBH32_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001154225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1514620.1"
                     /translation="MQDSSLNNYANHKNFILMLIILFLMEFARGMYILSYINFLPTVT
                     SIAVAITSLAFSIHFIADASTNFVIGFLLKKFGTKIVLTTGFILAFTSLFLVIWFPAS
                     PFVIIFSAMMLGIAVSPIWVIMLSSVEEDKRGKQMGYVYFSWLLGLLVGMVFMNLLIK
                     VHPTRFAFMMSLVVLIAWILYYFVDVKLTNYNTRPVKAQLRQIVDVTKRHLLLFPGIL
                     LQGAAIAALVPILPTYATKVINVSTIEYTVAIIIGGIGCAVSMLFLSKLIDNRSRNFM
                     YGVILSGFILYMILIFTLSMIVNIHIVWIIALAIGLMYGILLPAWNTFMARFIKSDEQ
                     EETWGVFNSIQGFGSMIGPLFGGLITQFTNNLNNTFYFSALIFLVLAVFYGSYFIANR
                     EKAK"
     gene            complement(12898..13407)
                     /locus_tag="NBH32_001002"
     CDS             complement(12898..13407)
                     /locus_tag="NBH32_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcG family protein"
                     /protein_id="HCD1514621.1"
                     /translation="MILGLALIPSKSFQEAVDSYRKRYDKQYSRIKPHVTIKAPFEIE
                     DGDLDSVIEQVRARINGIPAVEVHATKASSFKPTNNVIYFKVAKTDDLEELFNRFNGE
                     DFYGEAEHVFVPHFTIAQGLSSQEFEDIFGQVALAGVDHKEIIDELTLLRFDDDEDKW
                     KVIETFKLA"
     gene            complement(13601..14359)
                     /locus_tag="NBH32_001003"
     CDS             complement(13601..14359)
                     /locus_tag="NBH32_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1514622.1"
                     /translation="MSEFKTGKINKHVLYSNILNRDVTISIYLPESYNQLVKYNVILC
                     FDGLDFLRFGRIQRTYESLIKEARIDDAIIVGFHYEDVDKRREEFHPQGSRSHLTIQS
                     VGKEILPFIDSTFSTLKVGNARLLVGDSLAGSIALLTALTYPTIFSRVAMLSPHSDDK
                     VLDKLNQCANKEQLTIWHVIGLDEKDFTLPTNGKRADFLTPNRELAEQIKKYNITYYY
                     DEFEGGHQWKDWKPLLSDILLYFLRKNTDDQHYE"
     gene            complement(14504..16072)
                     /locus_tag="NBH32_001004"
     CDS             complement(14504..16072)
                     /locus_tag="NBH32_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine:cation symporter family protein"
                     /protein_id="HCD1514623.1"
                     /translation="MIEKLVTFLNEVVWSKPLVYGLLITGVLFTLRMRFFQVRHFKEM
                     IRLMFQGEKSPNGISSFQAIAMSLAGRVGTGNIVGVSTAIFIGGPGAVFWMWITAFLG
                     ASSAFIESTLGQIFKRVENNEYRGGPAYYIEYGIGGKFGKIYGIIFAIVTIISVGLLL
                     PGVQSNAIASSMHNAIHVPQWLMGAIVVVILGLIIFGGVRSIANVATAVVPFMAIIYI
                     LMAVIIICINIQEVPALFALIFKSAFGLQSAFGGIVGAMIEIGVKRGLYSNEAGQGTG
                     PHAAAAAEVSHPSKQGLVQAFSVYIDTLFVCTATALIILISGTYNVTDGTVNANGTPH
                     LIKDGGIYVENATGKDYSGTAMYAQAGIDKAFHGSGYQFDPTFSGVGSYFIAFALFFF
                     AFTTILSYYYITETNVAYLTRNQNNQVSSIFINIARVIILFATFYGAVKTADVAWAFG
                     DLGVGLMAWLNIIAIWILHKPAVNALKDYEIQKKRLGNGYNAVYQPDPNKLPNAVFWL
                     KTYPERLKQARAKK"
     gene            16414..17499
                     /locus_tag="NBH32_001005"
     CDS             16414..17499
                     /locus_tag="NBH32_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCD1514624.1"
                     /translation="MLNKVWFRTGIALIMLFILIKLFMEVHEVFTPIATIIGSVFLPF
                     LISGFLFYICLPFQNLLEKVGFPRWASITTIMLALFAIIGLIVAFVAPIIISNINNLI
                     SQTPALQKEAEQIIKFALAQMDKLPEDVTSRITNMVKSMGDGVTNILSNSLQYITSLI
                     STIFLLIMVPFFLIYMLKDHEKFIPAVAKFFKGERKVFFVDLLTDLNFTLKSYIQGQV
                     TVSVILGIFLYIGYSIIDLPYIPLLVLFAGVANLIPFLGSWLSFAPAAILGIIDSPTT
                     FIWVCIITLIAHQLEGNIITPNVMGKSLSIHPLTIIVVILAAGDLGGFTLVLIAVPLY
                     AVLKTVVSNIFKYRQRIIDKANSNVKD"
     gene            complement(17696..18466)
                     /gene="fabI"
                     /locus_tag="NBH32_001006"
     CDS             complement(17696..18466)
                     /gene="fabI"
                     /locus_tag="NBH32_001006"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCD1514625.1"
                     /translation="MLNLENKTYVIMGIANKRSIAFGVAKVLDQLGAKLVFTYRKERS
                     RKELEKLLEQLNQPEAHLYQIDVQSDEEVINGFEQIGKDVGNIDGVYHSIAFANMEDL
                     RGRFSETSREGFLLAQDISSYSLTIVAHEAKKLMPEGGSIVATTYLGGEFAVQNYNVM
                     GVAKASLEANVKYLALDLGPDNIRVNAISAGPIRTLSAKGVGGFNTILKEIEERAPLK
                     RNVDQVEVGKTAAYLLSDLSSGVTGENIHVDSGFHAIK"
     gene            complement(18744..20588)
                     /locus_tag="NBH32_001007"
     CDS             complement(18744..20588)
                     /locus_tag="NBH32_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monovalent cation:proton antiporter family
                     protein"
                     /protein_id="HCD1514626.1"
                     /translation="MEFLSLVIVVLAAFLTPIIVNRLNINFLPVVVAEILMGIVIGNS
                     FLNIVERDSILNILSTLGFIFLMFLSGLEIDFKAFKKDKRARQGQNDDESSIPGHLNL
                     ALTVFAFIMIISILLAYVFKWLGLVDDVLLMVIIISTISLGVVVPTLKEMNIMRTTIG
                     QFILLVAVLADLVTMILLTVYGAINGQGGSTIWLIGILVVFTAISYILGVQFKRMSFL
                     QKLMDGTTQIGIRAVFALIILLVALAEGVGAENILGAFLAGVVVSLLNPDEEMVEKLD
                     SFGYGFFIPIFFIMVGVDLNIPSLIKEPKLLIIIPILIVAFIISKLIPVMFIRRWFDM
                     KTTIASAFLLTSTLSLVIAAAKISERLNAISAETSGILILSAVITCVFVPIIFKKLFP
                     VPDEFNRKIDVSLIGKNQLTIPIAQNLTSQLYDVTLYYRKDLSDRRQLSDDITMIEIA
                     DYEQDVLERLGLFDRDIVVCATNDDDINRKVAKLAKAHQVERVICRLESTTDDTELVD
                     SGIEIFSSYLSNKILLKGLIETPNMLNLLSNVETSLYEIQMLNYKYENIQLRNFPFGG
                     DIIFVRIIRNNESIVPHGDTQLRYGDRLIVTGAKEYVDELKQELEFYF"
     gene            complement(20598..21983)
                     /gene="mgtE"
                     /locus_tag="NBH32_001008"
     CDS             complement(20598..21983)
                     /gene="mgtE"
                     /locus_tag="NBH32_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HCD1514627.1"
                     /translation="MSMNTDEKERVQEELYDQTLLDQYLENDDIDQFRDEFLALHTYE
                     QSEYFEDTTDENRQKIFQYLSPEEVANFFDQLDIDDDEYELLFDKMNATYASHILEEM
                     SYDNAVDILNELTKPKVASLLTLMNKDDANEIKALLHYDEDTAGGIMTTEYLSLKAHT
                     PVKEALLLVKAQAPDAETIYVIFVVDDDGKLVGVLSLRDLIVAENDAYIEDIMNERVI
                     SVNVADDQEDVAQVMRDYDFMAVPVIDYQEHLLGIITIDDILDVMDEEASEDYSRLAG
                     VSDIDSTNDSIIKTALKRLPWLIILTFLGMITATILGRFEKTLENVALLAAFIPIISG
                     MSGNSGTQSLAVSVRNITTGEINEQSKFRIALREAGSGVLSGVVCSTILFTIIVAIYH
                     QPLLALIVAGSLTCAMTVGTFVGSMIPLLMNKLNIDPAVASGPFITTINDIISMLIYF
                     GLATSFMAYLI"
     gene            complement(22004..22858)
                     /locus_tag="NBH32_001009"
     CDS             complement(22004..22858)
                     /locus_tag="NBH32_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1514628.1"
                     /translation="MKFKYHISQQETVKTFLARHDFSKKTVSAIKNNGALIVNDEPVT
                     VRKQLMPNDILEIHLPREIPSVNLIPYARKLEVLYEDAFIIIVTKPNNQNCTPSREHP
                     HESLIEQVLYHCQEHGENINPHIVTRLDRNTTGIVIFAKYGHIHHLFSKVNLKKIYTC
                     LVYGKTHTSGIIEANIRRSKDSIITREVASDGKYAKTSYEVINQNDKYSLCKVHLHTG
                     RTHQIRVHFQHIGHPIVGDSLYDGFHDKIHGQVLQCTQIYFVHPINKNNIYITIDYKQ
                     LLKLFNQL"
     gene            complement(22855..23664)
                     /locus_tag="NBH32_001010"
     CDS             complement(22855..23664)
                     /locus_tag="NBH32_001010"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HCD1514629.1"
                     /translation="MRYTILTKGDSKSNALKHKMMNYMKDFRMIEDSENPEIVISVGG
                     DGTLLQAFHQYSHMLSKVAFVGVHTGHLGFYADWLPHEVEKLIIEINNSEFQVIEYPL
                     LEIIMRYNDNGYETRYLALNEATMKTENGSTLVVDVNLRGKHFERFRGDGLCVSTPSG
                     STAYNKALGGALIHPSLEAMQITEIASINNRVFRTVGSPLVLPKHHTCLISPVNHDTI
                     RMTIDHVSIKHKNVNSIQYRVANEKVRFARFRPFPFWKRVHDSFISSDEER"
     gene            complement(23681..24316)
                     /locus_tag="NBH32_001011"
     CDS             complement(23681..24316)
                     /locus_tag="NBH32_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD1514630.1"
                     /translation="MNQWDQFLTPYKQAVDELKVKLKGMRKQYEVGEQASPIEFVTGR
                     VKPIASIIDKANKRQIPFDRLREEMYDIAGLRMMCQFVEDIDVVVNILRQRKDFKVIE
                     ERDYIRNTKESGYRSYHVIIEYPIETLQGQKFILAEIQIRTLAMNFWATIEHTLRYKY
                     DGAYPDEIQHRLERAAEAAYLLDEEMSEIKDEIQEAQKYYTQKRSKKHEND"
     gene            complement(24333..24680)
                     /locus_tag="NBH32_001012"
     CDS             complement(24333..24680)
                     /locus_tag="NBH32_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514631.1"
                     /translation="MRLYINEIKIKDDILYCYTEDSIKGLSEVGQMLVDSDNYAFAYT
                     LDDGKAYAYLIFVQETWTMLHENMTKKIIINDELELTEFHQELTYILDNIKGNNNYGK
                     EFVATVEETFDIE"
     gene            24866..>25444
                     /locus_tag="NBH32_001013"
     CDS             24866..>25444
                     /locus_tag="NBH32_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HCD1514632.1"
                     /translation="MATNHEIEFKQMITASIYNKLQEKYFKDSVLFKQVNYYIDTPDF
                     KLKEHRSALRIRVKDNQYEMTLKTPAKVGLLEYNYIVDIKPEMNLIISNDNLPDDIRQ
                     IIVEQFGVKDTTLSILGALTTYRQETKYKGDLLVLDKSEYFDTTDYELEFEVKDYNQG
                     LQKFQSLLHELNLEHHQPLNKLQRFFKKKETLS"
BASE COUNT         8332 a         4691 c         3523 g         8898 t
ORIGIN      
        1 ggtttttaga attgttaaac ttataattaa tttattacat agcaatattt acctgttttt
       61 aactacaaaa ttatcactac atgaaatacg ataattcgga tctcttaact tctctgcaat
      121 taatgtactc attgtttcat cgtatgattc atgtataata gcatttttca aataattctt
      181 gtagttacga taacaatagt cagtcattgt aactctataa ctcttagtca aatcaatttg
      241 atctaacatt actcgttgcc caggttcttg attcatatca atctcataat tgaatccttg
      301 ccacattgta cataacgttt catcaataat tgttaagctt aattgctcgt taacaaaatc
      361 taaatgtgaa taactatact ccaatatatc tttgatattt tgaccactaa ttgtcatatc
      421 cattggctta tctggatatg ggtatgcatg atacaaatct tcatttcgaa tctgtccact
      481 caaccccttc tctccgttca taggcacgtg gacacatgtt atatcattat caaatgcaag
      541 gtgaattgca tcatgtaata attgcgaaaa tggatgtgtt tgacagacta aatcttgtaa
      601 cccatttact gataacatca aacctttatc acttatgatt tcctgtgacc aataagccac
      661 tgcttttcta tcatagaatg ttaaatctaa taattcttga tcctcttcat actcatttaa
      721 gtcaatcact ttagaatcaa tactttcaac atcataagtt gttgttctct ttttaaaatt
      781 aatcgaaaga tgtacaagct cttttgcatc ttgaccagcc tgaacataat acgtttcatg
      841 atcttgacct actattgttt gatgctgatg agctgtaatc attaaatcta taacaccgag
      901 ttcttccatt aatttttcag cttcattcga acttgccttt ttatttttcg tgctattact
      961 aattttattc aatccaccat gataaattac aatcaaaaaa tctggctctt caacttcatg
     1021 gatatatcta atccatcgtt ttgatgccac taatgtcttt tcaatagata catcttgttc
     1081 catttcagaa tactcatttt ccattaaacc atctgctgtg acgcctacga tagcaatttt
     1141 taagtcacca aaatgtttaa tacaatatgg cgttgaaaaa tatggctcct tagtaacatt
     1201 gtattcaata tttgctgata accatggaaa acgtgccaaa gcaattgaac gagttaaaaa
     1261 tgataaacca aacttgaatt cacttggact cacaccgcta gcatcataat gcattctgtt
     1321 catcaacttt atcattggat gtcgtttata aggtgcaaca atagcataat aataggccgc
     1381 taacgagcca gctaaacttc cgccactatc taataaaatg acgcgatgat tttgtgctct
     1441 tacttggtta acataagtac cagctctata tatatttgaa ccataatcac catttaagaa
     1501 atgactatgc atatctgacg tagttaaaat ctctacatta atgttttcat ttttttccat
     1561 catgttacac ctctcattgt tactagttta ccattttcaa aaccaatcgt cttataaaat
     1621 aagttaaaaa tacaaactaa aaaagcaatc taagttccag ttaaaatcta cattaactaa
     1681 acttagattg ctattatatt atcctatttg aatacgttct tttggataat ggaatttatc
     1741 tggttctcga cgtcctgcta tcataatgat aaatgatatt aagccaacac gtcctataaa
     1801 catgagtacc attagtacga ccttggaaat atcactaata tcacttgtga caccaagcga
     1861 tagtccacaa gttccaaatg cagacatgac ttcaaaaaat acttgtaaaa atgtaagctt
     1921 accattttca gtagctgata taattagcat tcctaaaaat gttaaaattg tcgccattgt
     1981 aaatactgca aatgaacgtt gaatatccat aatgtgtact tctctattgt aaactttaat
     2041 ggatgtttta tcggcattat tactaaagtt aattaaaaac aaaattaaaa tagcgaatgt
     2101 tgttgtacga ataccgccac caaccgaact tggcgaagaa cctataaaca ttaaaatacc
     2161 catgataata ttcgtagggt cacttagtgt tgtcacatca attgtttgaa gacccgcact
     2221 tcttgtagtc gctgattgaa atagcgaata gaataacgat tgatgccaac ttaatccttt
     2281 gaacgcatgg ttatgttcaa ataatagaat ggctaatacc ccaacaataa ataaaaataa
     2341 atatgttgat gtcgtaattt tagtaaataa tgaaaatcta aaattagtaa ccctattttg
     2401 aatataagct ttaacttcta ataatactgg gaagccgatt gatcctaaaa ttattaaaaa
     2461 tataactatc gcttgtacaa aataatcatg tgcataagga attaatgact tacctgtaat
     2521 atctaatcca ccattggtag tggcagaaat agaaacaaaa acaccttgca tgattgcata
     2581 ttttaaatct ggattatctc gataaaagta aaatgctaat aacatggctc ctacgagttc
     2641 gattacaaat attgatttta caatatcaat aatcaatttg acggtaccac tcattgtgtt
     2701 tttgttatta tctaacataa ttaactgacg ttctctaatt ccaatatgtt tacctagtac
     2761 cacccataac atcgtaccaa ttgccatgac gccaattcca ccaatattta atatcacgag
     2821 gataattaat tgtccaaatg tagaataggt atcgacaata cttatcggag acaatccagt
     2881 aacactaatt ccggatacgg caacaaataa tgtgtcaatt ggatttactt ctacacctgg
     2941 tttatgaaca tacggtaagt ttaataataa aaatgcaatg acaattgcga cgatatagta
     3001 caatacaata ccttgttgag ggcttgatct ttttaaaaac tggctaaaaa ttgacacgtt
     3061 gttcacctca acattatttt agtttaatat taatttcttt ctctttacca tctctataaa
     3121 tcttcgctgt aattgatttc aagtcatctt tatgactaaa tataatctgc ctaaagcgta
     3181 aatcatcttc taaaagtttg ccatctaatt ctgtaattac atcacctttt ttcagaccag
     3241 attgatccgc taaaccattg ttgtcaactt gatctacaac aacaccgttc ttaacttttc
     3301 ctggcaattt aactgcttgt ctttcaaaac tatttagact ggcaatattc ttcattttaa
     3361 cacctacatc gggatagtca attttacctt ttgtttctaa accttttaca atcttttgta
     3421 cttcattaac aggtattgca aatgacatgt tttcaacgtt tggcatacta attttagctg
     3481 caactacacc aattaatttt ccttctctat tgacgacagc accacccgag ttacctggat
     3541 ttactgatgc atcaatttgg aaagctttca tcaacatatc atatttatta tctttatcga
     3601 aatcaatagg aacatttctg ttcagacctg aaacaatacc ttcagtcaca gtgcctttaa
     3661 agtctacacc aagtggatta cctacgacta atattggctc tcctaacact aaattatttg
     3721 aatctccaat agctatctct ttcactgaac tgtctgaaga agttgcttta acaacagcta
     3781 aatctgacca tttatcttta ccaagcactt tcccaacaac acttttatta ttcgagaaag
     3841 ttattttttg attttcttta tcaccgacaa cgtgtgcatt cgtaacaata tataacgtat
     3901 ctccagattt tttatataca acacctgatc ctacttcgtc ttgagatgct ttatctttag
     3961 gtaatgatga atcttttgat gtttcatttt caactgtaac gaccgattta actgtgttat
     4021 tggcattttt catcgttgtc gtatattttt gagcatctgt atcattatta tcattcatgc
     4081 gatcattttt attcacatta ttaaaaatgg cattcaaaat aacaattaga ataataatac
     4141 ccattagaat aactaattta ggccaataca tcatccaaaa ctttttcaaa atgtcactat
     4201 aattattatt tgtagcgcgc cctttgttca aatttgatgt agcctttttt tgattattat
     4261 ttgccgatgt ctttgtattt gttttttgac cgtttaattt atcatggtca ttaatagaaa
     4321 tatttctttt ttcagtgcta cgatgagatg cacttgactg actttgatat cccaccgttt
     4381 cagacgagtc tgcattttgt ttatgtttat cttcaacatg aattttttca tcaagttttc
     4441 gatcgtcatc tgtttttgat aatgatggtg tattgatatc attatcagga ttatcgtcaa
     4501 caatatgatt ttctctcata acgttatctt tatcatcact tatcgatggt gtatgatgac
     4561 ttaaatcatt ttcatgatta gacttgtcat tttgttcgct atcaccatat gtataggttt
     4621 ttgtttgctc attttgactt tgctgtcttt tcaatttctg ttcttccggc aaagttaatg
     4681 gcttctgatt atttacttca tcatcagatt ccgacaattt tggtactgta tttgatttgt
     4741 ctgatattac ttcagatttc agattactac ttttattttc atctgaactg tcttttttta
     4801 tttcctctgt ctgcatcgag ctgtctgaat cagttcttga agactcaaaa tcaattctag
     4861 tctctttatc atgttctgaa ggtttagaaa gtacatgttg ctcttcatga ccaactttgt
     4921 ttttatctga acttagcttt ctcatgtcag ttgattctat tttatcggtt gatttagttg
     4981 ataaagtcgc atcagaattt tgctcataat attttgattt ctctgtatct tggtccactg
     5041 aatctaatga attttgtttt ttaaatacat cgtctgttaa ataatgatct ctgctatatg
     5101 ctgacttact atcctgatta cttttacttt cttcagtttt attctcatta acatcacggt
     5161 ttctctgttc taaatgcgat gataaactgt ttttaaaacg ttcgtgctta tcaattgaat
     5221 ctttatgtat ttgcttatct gctttttttg atgttgtatg atctatattt tgtttatctt
     5281 gatgttgttt taaattttct tctctgtctt cgttgtggaa gaattcacga cgcttacgtc
     5341 ggtactgact tttaggaatt acatgttttt taccaatatc cactaatgtc cactccattc
     5401 aataaaatca cttttcatta tgacacatta acacttaatt tgctttaaaa tctatataat
     5461 ttctattgaa aaaatgctaa atataaaagg cacattttct taatgctata tcgcatattt
     5521 tgattcatat tcttcaaaat ccatttttca taaatcactc aattaaatga aaaaggacaa
     5581 ccttaaacta aattaagatt gtccgcttca catcaattat ttatgcgatt ttttattttt
     5641 aacaactgaa cctaaccaac cgataattac taatccaatt agtactgtcc agaaaataga
     5701 ttgccataat acgccatgtg ggaagtgctc aggcaataca gcgatgtctg ggtgcgctaa
     5761 taccatgaca actaatttaa cacctaccca accaacgatc gcgaaggctg caccttcaag
     5821 tcctggatat ttgtttaata gctctacaaa ccatgttgct gcataacgca ttagaataac
     5881 accaatcatt ccacctagga acatgactac gaactgacct aagtccatac caccaaagtg
     5941 aataccaact ttaggaagtg ttacagcaat agctaaagca gcaagcatag aatctatggc
     6001 aaatgcgata tctgcaaatt ctattttcaa cacagttccc cagaatgatt tgttacttgc
     6061 tttaatctct tcaccagatt catcataatg atgatcatct ccagcttcag gactttcaat
     6121 ttctgggtgt ttaaagaact gccacagatt tttgattgac atataaatta agtaaaccgc
     6181 tcctgcagct tgaataaacc aaaagttcgc gataatacta attaagaata atgctaaaaa
     6241 tctaaataca aatgcaccta acaaaccgta aaacaaagct tttttacgtt gttcgggtgg
     6301 taagtgctta accattacag ccataacaat cgcgttatct gctgctaata agccttctaa
     6361 aaatactaaa acgacaagta cccataaata aggtaagatc aaacttggat ccattaacat
     6421 gtacaactcc tttaaataaa taagttaagt acaaacaaaa agacctatgc taaataaata
     6481 gcaaaggtct cactaagcaa tttaaattgc ccactttacc ggaagatatc ttcgtaatga
     6541 cgataaagtg tcgaatcatt tatacggtca tgattcttaa gttactcccc tctgacaaaa
     6601 tgccataggt tattcatttg atacatcata gtataccttt ttcttttaaa atatacaatt
     6661 tatttttctt caacgaaaca attattttta atgtaacaaa ttcgattata caattgagct
     6721 gttaaagtaa actatacaat ttaatttcag ggaatttctc ttcaaaccat cttgttgcaa
     6781 attcattttc aaataagaat actaaatcgt catatctatc tttcactaaa atcgatcttg
     6841 atgtgttcat cttatctgta atttggtctt cattttcaat ccaacgtgca attttacggc
     6901 ctactggctc cataacaaca tcaacattat attcgttttt cattctatgt tcgaaaactt
     6961 caaattgtaa ctgaccaaca gcacctaaaa taatttggtt tgtgtgtaat gttttatagt
     7021 attgaatcgc accttcttgt actaattgtt caataccttt atggaaatgc ttctgtttca
     7081 tgacgttttt agcagaaact ttcataaaaa tttctggcgt aaattgtggt aaatcttgga
     7141 aactgtaggt ttgttttcca ccaactaaag tatctccaat ttgataatta ccagtatcat
     7201 atagtccaat gatatcgcct gctacagcat gattcacagt ttctttatcg tctgccataa
     7261 atgacgttga acgtgtgatc ttttgctttt tattagtacg ttgcaaagta acatccatac
     7321 cacgttcaaa tgcaccacta acgacacgca taaaggcaat tctatcacgg tgtttagggt
     7381 ccatgttggc ttgaatttta aagataaatc ctgaaaatga atcatcaaac gggcttactt
     7441 caacgtcttc ttttgtttgt ctcgcatttg gcattggcgc aaaatcaaca tatgcattta
     7501 agaaattttg tacaccaaag ttagctaaag ctgaaccgaa aaatacaggt gttaagtctc
     7561 cactcaacag cgcgtcatta tcaaaggctt cacccgcttc ttcaaccaac attaattctt
     7621 caatcgcttg ttcaaaagca ctatcatttg taattgcatg atcttcttcc aactcaaaat
     7681 catcattcaa atgtaatata ttttcttcat ctctaaatgg ttcaattgtt ttagactttc
     7741 tatcaatgat gccaaagaaa ctttgtccca taccaattgg ccaattcata gggtatgttt
     7801 caatatttaa tgtctcttcg atttcatcta ataattcaaa tggttcttta cctactcggt
     7861 ctaatttatt aatgaatgta aagattggaa taccacgcat tttacaaact ttaaataact
     7921 tcaatgtttg tggttcaata ccttttgcac agtctatgac catgacagca ctgtcaactg
     7981 ccattaatgt tctatacgta tcttctgaaa agtcttcatg tcctggtgta tctaagatat
     8041 tgattttata atcatcgtaa tcaaattgca ttactgaact agttacagaa ataccacgct
     8101 cttgttcaac tttcatccag tcacttgtcg caaatttacc agtcttcttc cctttaactg
     8161 tacccgcttc acgaatagca ccactgaagt acaatagttt ttcagttaac gttgttttcc
     8221 ctgcatcggg atgtgaaata atcgcaaaag tctttctaga ctcaacttct tgctttaagt
     8281 tcattagtct ttctcctttt tatatgtttc tatccaatct gttagttgtg atgcaatcga
     8341 ttctgcctct tcctcttctt taaaaacaaa caattgcatg agtattgctt caaaacaagt
     8401 ttcttcatct aatgtcattg ccatctgata tgaccaaaac caatctggta tagcaatgat
     8461 tatctggtta tttaatgctt cgaataaata aacatcaata gttttacctt catcaataaa
     8521 tcttttattg atcaacaggg ccaccaccga actttttgta agctgcttca aggccaatta
     8581 aatcatctcg atgtggcacc ttaatatgcc ctggcatgat ttgatatggt tctcttcctt
     8641 ttgatgctaa aacgacagta tccccaggct cagctatgtc aattgcatgt tttatccctt
     8701 ctgcacgatc atcaaattca atatagtttt gatgtgttgc acctttggct aattctgccg
     8761 ttaacatttt cgggtcatca tttgccggat tatccggtgt gaaaatgaca taatctgcac
     8821 gacaggcaac tcgccccatt tcaggcgttt tagttaaatc acgttcgcct gccataccaa
     8881 ctaaaaatat caacttttgc tttacaaaag gctgtactgc atcgattaat ttgttcatac
     8941 catcagctgt atgtgcataa tcgataatta aatcaatagg taacgaagga tctaaaactt
     9001 ctaatcgccc ttcaacaggt tctaaatttt caacagcttt aataatcgtt tctaaagatg
     9061 tacctttact ccacaccgca atcattgccg ccataatatt agaaatatta aacttaccaa
     9121 cataaggcga ttttactggg taagttccaa aaggcgttac aaaatcaaag ctgacacctt
     9181 gtaaagattc ttgaatattt ttagccataa attgggcttc ctcatcaatt ccataactaa
     9241 atacttcata aggcgtcact gttcttaaat actcagaaaa tgaatcgtca ttgtttaaca
     9301 cgacatactt ttctttcgac aaatcttcac ctaattgact aaacaataaa gacttcgcgt
     9361 gtccgtatgc ttccattgtg ccatgaaaat ctaaatggtc ttgtgttaaa tttgaaaata
     9421 ttgcaacgtc aaattcaacg cctcgcagtc gtcctaatac taagccatgg cttgatactt
     9481 ctaatgtcat agattcagcg cctgcatcaa ctgcttcttt aattttctta gttaaagaaa
     9541 ctgtttctgg tgtcgtattt gcaccttttg tctttgtttc attaatttgg aaaccattag
     9601 ttcctaaata tgcactattt ttttgtaact ttctttgaat taaatgaatc atcgtcgcaa
     9661 tagaagtttt accatttgta cccgttacac caaatgtcac taactgatga ctcggataat
     9721 catataatgt gtgtgctaga atactagcta ctcttaatgt gtccggcaca accacttgtg
     9781 ttacgttagc tggtaatgat tgttctttat tgaccactac caacttacac ccttgatcag
     9841 ctacattttg acagaactta tgactgtcta cagtatatcc aactgaagcg acaaaaatgc
     9901 taccttctct cgctgtacgt gaatcagtag tgatatcatc tatttgttgt tctaaagaac
     9961 ccaatacacg ctttactttt actttcttaa acaacgtact tgcatccaaa ctgaaccgct
    10021 cctttttcta caatatgttt attatacacg ttttgttgat gttaataaat ttataatttc
    10081 actgttatat cctactaatc agcattttct aatttctact attaaaaata aaacatacgg
    10141 tattatgaat tagttttaca attatggctc atcttaaatt attaaaacca tcttgtaaca
    10201 tggtataata tcataattgt ttccaaaaat atgctataat tgtatactgg ataattttaa
    10261 atatacaaat aaaacataga acaataaatt gctttataaa ataacgtttt attttgtatc
    10321 aattcatcta ttgattaaaa ttgctataaa tgtaacgact ttgcgtcttt atttctttta
    10381 taatgccgta aaataagtta cacttaaata aaattttcaa actaacggag ggtggctatt
    10441 aatggttact caaaataaaa agatattgat tattactggc tcattcggta acggtcatat
    10501 gcaagttaca cagagtatcg ttaatcaact taatgatatg aatctagacc atttaggcgt
    10561 cattgagcac gatttattta tggaagctca tccaattttg acttctattt gtaaaaaatg
    10621 gtatatcaat agctttaaat attttagaaa tatgtacaaa gggttttatt acagccgccc
    10681 agataaacta gacaaatgtt tttacaaata ctatggactt aataagttaa ttaatttatt
    10741 gataaaagaa aagccagatt taatattatt aacgtttcct acaccagtta tgtcggtact
    10801 aactgagcaa tttaacatta atattccagt tgctacagtg atgacagact atcgcttaca
    10861 taaaaactgg attacgccgt attcaacaag atattatgtg gcaacaaaag aaacgaaaca
    10921 agacttcata gacgtaggta ttgatccttc aacagttaaa gtgacaggta ttcctattga
    10981 taacaaattt gaaacgccta ttaatcaaaa gcagtggtta atagacaaca acttagatcc
    11041 agataagcaa actattttaa tgtcagctgg tgcatttggt gtatctaaag gttttgacac
    11101 gatgattact gatatattag cgaaaagtgc aaatgcacaa gtagttatga tttgtggtaa
    11161 gagcaaagag ctaaagcgtt ctttaatagc taagtttaaa tctaacgaga atgtattgat
    11221 tctaggttat accaaacaca tgaatgaatg gatggcatca agtcaactta tgattacgaa
    11281 acctggtggt atcacaataa ctgaaggttt cgcccgttgt attccaatga ttttcctaaa
    11341 tcctgcacct ggtcaagagc ttgaaaatgc cctttacttt gaagaaaaag gttttggtaa
    11401 aatcgctgat actccagaag aagcaataaa aattgtagca agtttaacga acggtaatga
    11461 gcaattaact aacatgatta gtacaatgga acaagataaa attaagtatg ctacacagac
    11521 aatatgtcga gacttacttg atttaatcgg tcattcatca caaccacaag agatttatgg
    11581 aaaggttcct ttatatgcaa gattcttcgt taaataatta cgctaatcat aaaaatttca
    11641 ttttaatgct tattatctta tttttaatgg aatttgcgag aggcatgtat atcctaagct
    11701 atattaattt cttgccaaca gttacatcca ttgctgtagc aataacgtct ctagcatttt
    11761 caatacattt tattgctgat gcatctacaa actttgttat aggattttta ctgaaaaaat
    11821 tcggcacaaa aatcgtttta acgactggat ttattttagc atttacaagt ttatttttag
    11881 ttatttggtt tccagcatca ccttttgtaa tcatatttag tgctatgatg ctcggtattg
    11941 cagttagtcc tatttgggtc attatgttat ccagtgttga agaagataaa cgtggcaaac
    12001 aaatgggcta tgtttatttt tcatggttgt taggcctatt ggtaggtatg gtttttatga
    12061 atctacttat caaagtgcac ccaacacgct ttgccttcat gatgtcttta gtcgttttaa
    12121 ttgcatggat attatattat tttgtcgatg taaagttaac aaattataat actcgtccag
    12181 ttaaagctca attgagacaa attgttgatg tgacaaagcg ccatttattg ttgttccctg
    12241 gtattctttt acaaggtgcc gcaattgctg ccctagttcc tatattacca acatatgcta
    12301 ctaaggttat taacgtaagc acaattgaat atactgttgc aatcattatt ggtggtatcg
    12361 gctgtgcagt ttcgatgcta tttttatcga aactaataga taaccgcagc agaaacttta
    12421 tgtatggtgt tattctaagc ggatttattt tatacatgat attaattttt actctatcta
    12481 tgattgttaa tattcacatc gtgtggatta tcgctttagc tatcggtcta atgtatggca
    12541 tcttattacc agcatggaat acctttatgg cacgatttat caaatcagat gaacaagaag
    12601 aaacttgggg tgtttttaat agtatccaag gattcggctc aatgataggg ccattatttg
    12661 gcggattaat tacacaattt accaacaatt taaacaatac attttatttc tcggcattaa
    12721 tctttttagt cttagctgta ttttatggaa gctactttat agcaaataga gaaaaagcta
    12781 agtaaggtta aaacgtattt taaaataaag aaaatggtca tttttattac attcaataaa
    12841 aatgaccatt ttcttattaa ttcaaacata ctacgattaa caatactatt atgttactta
    12901 agctaattta aacgtttcaa taactttcca tttatcttcg tcatcgtcaa aacgtaacaa
    12961 agttaattca tcgataattt ctttatggtc taccccagct aatgctactt gaccaaaaat
    13021 atcttcgaat tcttggctag atagtccttg tgctattgta aagtgtggca caaaaacatg
    13081 ttcagcttct ccatagaaat cttctccatt aaagcgatta aacaattctt ctaagtcgtc
    13141 cgtcttcgca actttaaagt aaatcacatt gttcgttggt ttgaagctag aagcttttgt
    13201 agcatgaact tctactgctg gtataccatt aatacgagct ctaacctgtt caatgacaga
    13261 atctaaatca ccatcttcaa tttcaaatgg cgctttaatt gtcacatgtg gtttaattcg
    13321 tgaatactgt ttatcatatc ttttacggta agaatccacc gcttcttgaa atgactttga
    13381 tggaattaat gctaatccta aaatcatctg aattcctcct ttgtttaacc tattgctatt
    13441 atatcaaatt tctttgaaaa ccgttacatt aaaaataatt cattatacat cttttgcaat
    13501 ttcgacaaat ttaaattaaa tcaaacagta aaatctttta taaattatat tatagaccaa
    13561 actatatgaa gacaattcat actaaatcta ctaatgtaaa ttactcataa tgttgatcat
    13621 ctgtgttttt acgtaaaaaa tacaataata tatctgacag caatggtttc caatctttcc
    13681 attggtgacc accttcaaat tcatcgtaat aataagttat attatatttc ttaatttgtt
    13741 cagctaattc tctattcggt gttaagaaat cggcacgctt accatttgtt ggtaaagtaa
    13801 aatctttttc atctagacca atgacatgcc aaattgtcaa ttgttcttta ttagcacatt
    13861 gatttagctt atctaatact ttgtcatctg aatgtggact taacattgct acacgactaa
    13921 aaatcgttgg ataggtcaac gccgttaata aggcaatact acccgctaaa ctatccccta
    13981 ctaataacct tgcattacct actttcagtg tagaaaacgt cgagtcaata aatggcaata
    14041 tttctttacc gactgattga atagttaaat gagaacgact tccttgtgga tgaaattcct
    14101 ctctacgctt atcaacgtct tcataatgga atccaacaat gatcgcatca tcaatacgcg
    14161 cttctttgat taacgattca tatgtacgtt gtattctccc gaaacgtaaa aaatctaatc
    14221 cgtcaaagca aagaatgaca ttatatttaa caagttgatt ataagattct ggtaaataaa
    14281 tacttatcgt gacatctcta tttaaaatat tactatataa aacatgttta ttaatcttac
    14341 cagttttaaa ttctgacata aaaaagtgcc ccctagtagt tgtattgtta ttgtaccaag
    14401 agacactata tttttaaaat tcaaatttct gagcacctaa aattatcaat gctgtttctt
    14461 tttttatcaa atgatcacta catatactat aaacaaaagt agattacttt ttggcacgtg
    14521 cttgttttaa acgttctgga tatgtcttca accaaaagac agcattaggt aatttattcg
    14581 gatcaggttg ataaactgca ttataaccgt ttcctaaacg tttcttttga atttcataat
    14641 cttttaaagc atttacggca ggcttatgta aaatccaaat cgcaatgata tttaaccaag
    14701 ccattagacc tacacctaaa tcaccgaatg cccatgctac atcagctgtt ttaactgcac
    14761 cgtaaaatgt agcgaacaaa ataatcacac gagcaatatt aataaatatc gatgaaactt
    14821 gattattttg attacgcgtt aaataagcaa catttgtttc tgtaatgtag tagtacgaca
    14881 aaattgtagt aaatgcaaag aagaataaag caaatgcaat aaagtacgaa cctacgccag
    14941 agaaagtagg atcaaattga taaccactgc catggaacgc tttatcaatg ccggcttgtg
    15001 catacatcgc agtacctgaa taatctttac ctgttgcatt ttcaacataa ataccgccat
    15061 ctttaattaa atgcggtgtg ccattcgcat taaccgtacc atcagtcaca ttatatgtac
    15121 cagaaataag tataatcaga gcagttgcag tacatacaaa taatgtatca atataaactg
    15181 aaaatgcttg tactagacct tgtttacttg gatgtgatac ttctgctgcc gctgctgcgt
    15241 gtggacctgt accttgacca gcctcatttg agtataatcc acgtttaaca ccaatctcta
    15301 tcattgcgcc aacgatacca ccaaaagcag attgtaatcc aaatgctgat ttgaaaatta
    15361 atgcaaataa cgctggcact tcttgtatat tgatacaaat gataatgaca gccatcagta
    15421 tgtaaataat tgccataaat ggtacaacgg ctgttgcaac attggcaata ctacgtacac
    15481 caccaaaaat aattaatccc aaaataacta caacaatagc acccattaac cattgtggaa
    15541 catgaatcgc attatgcata gaacttgcta tagcgttaga ttgcacacca ggaagcaata
    15601 gacctactga gataatcgta acaatagcaa agataattcc gtaaatttta ccaaatttac
    15661 caccaatacc atattcaata taatacgctg gtccaccacg gtattcatta ttttcaactc
    15721 tcttgaatat ttgaccaagt gtagattcaa taaaagcact acttgcacct aaaaacgcag
    15781 taatccacat ccaaaatact gcaccaggtc ctcctataaa tattgcagta gatacaccga
    15841 caatattacc tgtaccaacc ctgcctgcta aagacatggc tatcgcttga aaacttgaaa
    15901 taccattagg agacttctct ccttgaaaca ttaatcgaat catttcttta aaatgtctaa
    15961 cttgaaaaaa tcgcatacgc aatgtaaata gcacaccagt aattagcaaa ccataaacta
    16021 atggcttact ccaaacaacc tcatttaaaa aggttactaa tttttcaatc ataagattcc
    16081 ccctattgtt taaacataaa aatattatac gattaagcac gagacacttc aatatatttt
    16141 tcagaatatt cttataaata ttaatatgat catttcatta tttaaacacg tttaatatta
    16201 aaataagtta ttcatataaa ctggtgctgt ttgatccaat tgcagactta cgagtcattg
    16261 aaaagtctca caaactattg aaagtaaata tcttaaaata gaaagtgaat ggttaattta
    16321 agtatattta aaaaatatta acctttttaa gcactgctat ttaggatata ctaaataata
    16381 actaagttta gaaaaatagg aggaacatcg tttatgttaa acaaggtttg gttccgaact
    16441 ggcatcgctc tgattatgct gttcattctc atcaaactat ttatggaagt gcacgaagta
    16501 tttactccaa tagctacaat catcggttct gtatttcttc catttttaat tagtggtttt
    16561 ttattttata tctgtctacc ttttcaaaac ttacttgaaa aagtcggttt tccaagatgg
    16621 gctagtataa caacaatcat gttagctttg tttgctatta tcggcttaat tgttgcgttt
    16681 gttgcaccaa tcatcatttc aaatatcaac aatttaatta gtcagacacc tgccctacaa
    16741 aaagaagcag agcaaattat taaattcgca ctagctcaaa tggataaatt acctgaagat
    16801 gtaacaagta gaattacaaa tatggttaaa tcaatgggtg atggcgttac taatatttta
    16861 tctaattcat tacagtacat tacatcatta atttcaacca tcttcttatt gattatggtt
    16921 ccattcttct taatttatat gttaaaagac catgaaaaat ttatcccagc tgttgctaaa
    16981 tttttcaaag gtgaacgtaa agtatttttc gtagatttat taactgattt aaactttaca
    17041 ttaaaatctt acattcaagg tcaagtaact gtcagtgtta ttttaggtat attcttatat
    17101 atcggttatt caattattga cttaccatat attcctttat tagttctatt tgctggtgtc
    17161 gcaaacttaa ttcctttctt aggttcatgg ttatcatttg cacctgcagc aatactcggt
    17221 attatagata gtcctactac atttatatgg gtttgtatca ttacattaat tgcacaccaa
    17281 cttgaaggta atatcattac gccaaatgta atgggtaaat ctttaagtat ccatccttta
    17341 acaattatcg ttgttatttt agcagcagga gatttaggtg gctttacatt agttctgatt
    17401 gcagtgccat tatatgctgt acttaaaacg gttgttagta atattttcaa ataccgccaa
    17461 cgcattattg ataaagcaaa cagtaatgtt aaggactaat tctgtggatg tcttttaaga
    17521 atataagata ctatctcctc aaaagttgaa actacagctt ttgaggagat ttttttgtgc
    17581 ataaaaaaaa tcagtcagat gaaatatcaa ataattttcc atcagtccga ttattataaa
    17641 agcaaaaaag ctttgctcac atatatgata acgtgaacaa agctgttgaa tgatattatt
    17701 taattgcgtg gaatccgcta tctacatgaa tattttcacc tgtaacgcca cttgataagt
    17761 cacttaataa gtaagccgct gttttaccta cttctacttg atcaacgtta cgttttaaag
    17821 gtgcacgctc ttcgatttct ttaagaattg tattgaaacc acccacacct tttgcactta
    17881 atgtacggat tggaccagct gaaattgcat taacgcgaat attatcagga cctaagtcta
    17941 atgctaaata tttaacattt gcttctaagc tcgctttagc aacacccatc acattataat
    18001 tttgaactgc gaattcgcca cctaaatatg ttgttgcaac aatgctacca ccttctggca
    18061 ttaatttttt agcttcatga gccacaattg ttaatgagta agaactaatg tcttgagcta
    18121 acaagaagcc ttcacgtgaa gtttcagaaa agcgtccgcg taagtcttcc atattagcaa
    18181 atgcgattga atgatataca ccatcaatat tgccaacatc tttaccaatt tgctcaaaac
    18241 cattaataac ctcttcatcg ctttgaacat caatttgata taagtgcgct tctggttgat
    18301 ttaattgttc taataatttt tcaagctctt tacggctacg ttctttacgg taagtaaata
    18361 ctaatttagc acctaattga tctaaaactt tagcgacacc aaaagcaata ctacgcttat
    18421 tagcgattcc catgatgaca tatgttttgt tttcaagatt taacatgtaa gataactcct
    18481 tataataatt aatttaacgg atagaatatc taatcccagt tttaactaac tttttattcg
    18541 tcaatacatt ttaacactat tatgactttt taataaagtt atttaaacgt attacaacga
    18601 taaatgtttg atttagtcct ttctgtatca aacttgattt acgtaaaaag catttattta
    18661 tcaaaatcat atacatacta gcattttaaa atcattaatt ttacgtcgta ctaaaatacg
    18721 tctgcatttt aatatcatta ttgttaaaaa taaaattcta actcttgctt caattcatca
    18781 acgtattctt tagccccggt aacaattaag cgatctccat atcgcaattg tgtatctcca
    18841 tgcggaacaa tcgactcatt attacggata atacgcacga agatgatgtc tcctccgaat
    18901 gggaaattac gtaattgaat attttcatat ttataattta acatttgaat ttcatatagt
    18961 gatgtttcaa cattacttaa taaattcaac atgttaggtg tttcaattaa accttttaat
    19021 aagattttat tacttaagta gctactgaaa atttcaatac ctgaatcaac taactctgta
    19081 tcgtccgttg tgctttcaag tctgcaaatg acacgctcaa cttgatgtgc tttggctaat
    19141 ttagcaactt ttcggttaat atcatcgtca ttcgtggcac aaacaactat gtctcggtca
    19201 aacagaccta gtcgttctaa aacatcttgt tcataatcag caatttctat catcgtgata
    19261 tcatccgaca attgacgacg atcactcaaa tctttgcgat aatataatgt cacgtcatat
    19321 aactgagatg ttaaattttg cgctatagga atcgttaatt gatttttacc aattaaacta
    19381 acatcaattt tacggttaaa ctcatctgga actggaaaca gttttttgaa aataatcgga
    19441 acgaatacac atgtaatgac tgcgcttaaa attaatatac ctgacgtttc agctgaaata
    19501 gcatttaatc tttctgaaat tttggctgca gctatcacga gcgataatgt tgatgttaat
    19561 aaaaatgctg atgcaatcgt tgttttcata tcaaaccaac gtcgaataaa catgactgga
    19621 attaattttg aaatgataaa tgcaacgatt aaaatcggta tgataattag taatttcggt
    19681 tctttaatta atgaaggtat gtttaaatct acaccaacca ttataaagaa aataggaata
    19741 aagaacccat aaccaaatga gtctaacttt tcaaccattt cttcatctgg atttaataat
    19801 gaaacaacga cacctgctaa gaatgcacct aatatatttt ctgcgccaac tccctctgct
    19861 agggctacta ataatattat taatgcaaac accgcacgaa taccgatttg cgtcgtacca
    19921 tccatcaatt tttgtaaaaa tgacattctt ttaaattgaa cacctaaaat atatgaaatt
    19981 gctgtgaaaa caactaatat acctattaac catattgtac tgccgccttg accattgatt
    20041 gcaccataga ccgttaataa aatcatagtt actaagtccg caagtactgc tactaatagg
    20101 atgaattgac ctatagttgt tctcataata ttcatttctt ttaaagttgg aacaactacg
    20161 cctaaggaaa tagttgaaat gataatgacc attaataaca catcatccac taatccaagc
    20221 catttaaata catacgctaa cagaatcgaa ataatcataa taaatgcaaa tacagttaac
    20281 gctagattaa gatgccctgg aattgaggat tcatcatcat tttgtccttg acgtgcgcgt
    20341 ttatcttttt taaaagcttt aaaatcaatt tctaaaccac ttaaaaacat taaaaagata
    20401 aagcctaacg ttgataaaat atttagaatt gaatcccttt ctactatatt tagaaatgaa
    20461 tttccaatca caatccccat caaaatttct gcaacaacaa ctggcaagaa attaatattt
    20521 aatcgattga caattattgg agttaaaaac gctgctaaaa caacaataac taaagataaa
    20581 aactccataa ctcctcctta aattaagtaa gccataaatg atgtagctaa accaaaataa
    20641 atcaacatac taataatatc attaattgtt gtaataaatg gtccactagc cactgctgga
    20701 tcgatattta atttattcat caataatgga atcatcgaac ctacaaacgt ccccaccgtc
    20761 atcgcacaag ttaaacttcc tgcaacgatt aatgctaaaa gtggctgatg atatattgca
    20821 acaataattg taaataatat tgttgaacat acaacacccg ataatacacc acttcccgct
    20881 tctcttaatg caattctaaa tttactttgc tcattaattt cccctgtcgt aatgttacga
    20941 actgaaacgg ctaaagattg tgtacctgaa tttcctgaca taccactaat aataggaata
    21001 aacgctgcga gtagcgctac attttctaat gttttttcga atctccctaa aattgtcgca
    21061 gtaatcattc ctaaaaatgt taaaataatc aaccatggta aacgttttaa tgctgtttta
    21121 atgattgaat cattagtcga atcgatatct gaaaccccgg ctaaacgaga gtagtcttca
    21181 ctagcctctt catccataac gtctaaaata tcatcaatcg tgatgatacc aagcaaatgt
    21241 tcttggtaat ctataacagg tacagccatg aaatcatagt ctctcataac ttgagcaaca
    21301 tcttcttggt cgtctgctac attcacacta atgacacgtt cattcataat atcttcaata
    21361 taagcatcat tttcagctac aattaaatct cttagtgata aaacacctac taatttacca
    21421 tcatcatcaa cgacaaatat aacatatatt gtttctgcgt ctggcgcttg cgctttgacc
    21481 aataataacg cttctttaac aggcgtatgc gctttaagtg ataaatactc cgtcgtcata
    21541 ataccgccgg ccgtatcctc atcatagtga agtaatgctt tgatttcatt cgcgtcatct
    21601 ttattcatca atgttaaaag actagcaact tttggtttag tcaactcatt taaaatatct
    21661 actgcattgt cgtatgacat ttcttctaat atgtgacttg cgtatgtcgc attcatttta
    21721 tcaaatagca actcatattc atcgtcatca atatctaatt gatcaaagaa atttgcaact
    21781 tcttcaggtg ataaatattg aaaaatcttt tgtctatttt cgtcggtagt atcttcaaaa
    21841 tactcacttt gttcatatgt gtgtaatgct agaaattcat ctctaaattg atcaatatca
    21901 tcattttcta agtattgatc taataaagtt tggtcgtata attcctcttg aacacgctct
    21961 ttttcatctg tgttcattga catacttaca ccccctgtgt gaattagagt tgattgaata
    22021 atttaagtaa ttgcttataa tcaattgtaa tataaatatt gttcttattg attggatgaa
    22081 caaaatatat ttgcgtacat tgcagtactt gaccatgaat tttgtcatga aaaccatcat
    22141 acaaagaatc tcccacaatt ggatgcccaa tatgttgaaa atgtacacga atttgatgtg
    22201 tacgtcccgt atgcaaatga actttgcata aactgtattt atcattctga tttattactt
    22261 cataagatgt tttagcgtat ttaccatccg aggcaacttc tctagttata atactatcct
    22321 ttgaccgtct aatattagct tcaataatac cagatgtatg ggttttacca tatacaaggc
    22381 aagtatatat ttttttcaag tttactttag aaaataaatg atggatatgt ccatatttag
    22441 cgaatatcac aataccagta gtattacgat ctagacgcgt aacaatatgt gggttaatat
    22501 tttcaccatg ttcctgacaa tgatatagta cttgttcgat taaactttca tgaggatgtt
    22561 ctctcgaagg cgtacaattt tgattgtttg gtttagtaac tatgatgata aaagcatctt
    22621 catacaatac ttctagctta cgagcataag gtattaaatt aacactcggt atttctcgtg
    22681 gtaaatgaat ttctaatata tcatttggca ttaattgctt acgcactgtg actggttcat
    22741 cattaacaat taaagcgcca ttatttttaa tggcgctcac tgtcttctta gaaaaatcat
    22801 gtcgtgctaa aaaagtttta acagtttctt gttgtgatat atgatactta aatttcatcg
    22861 ttcttcatca cttgatatga aagaatcgtg tacacgtttc cagaatggga atggtctaaa
    22921 acgtgcaaac ctcacttttt catttgctac acggtattgt attgaattaa catttttatg
    22981 tttgatacta acatgatcta tcgtcattct aatggtatca tgattaactg gtgatattaa
    23041 acatgtatga tgctttggta atacaagtgg tgatcctacc gttctaaaca cacgattatt
    23101 tattgaggca atttctgtaa tttgcattgc ttcaagtgaa ggatgtatca gtgcgccacc
    23161 tagcgctttg ttataagccg ttgaacccga aggtgttgat acacataatc catcgcctct
    23221 aaatcgctca aagtgtttcc ctcttaagtt aacatccaca acaagtgttg agccattttc
    23281 agttttcatc gttgcttcat ttaatgctaa ataccttgtt tcatatccgt tgtcgttgta
    23341 tctcataata atttcaagca atggatattc aatgacctga aactctgaat tattaatttc
    23401 gatgattaat ttttcaactt catgaggtaa ccaatccgca taaaatccta aatgacctgt
    23461 atgaacccca acaaatgcca cttttgataa catgtggcta tactgatgga atgcttgtag
    23521 taatgtacca tcaccaccaa ctgaaataac aatttcagga ttttcactat cctcaatcat
    23581 gcgaaaatct ttcatatagt tcatcatttt atgctttaag gcattagact tggagtcacc
    23641 tttagttaaa attgtataac gcatgattta taacacctcg ttaatcattt tcatgttttt
    23701 tagaacgttt ttgcgtgtaa tatttttgag cttcctgaat ttcatcttta atttcagaca
    23761 tctcttcatc aagtaaatac gctgcttctg ccgctctttc caaacgatgt tgaatttcat
    23821 ccggataagc accatcatat ttatatcgta aagtatgttc aatcgttgcc cagaaattca
    23881 ttgctaatgt acgaatctga atctcagcca atataaattt ttggccttgt aatgtttcaa
    23941 ttggatattc aataatgaca tgatacgagc ggtaaccact ttctttagtg ttacgaatat
    24001 aatctcgttc ttcaattacc ttaaaatctt ttctttgtct taaaatattg acgacaacat
    24061 caatatcttc tacaaattgg cacatcattc ttaaaccagc gatatcgtac atttcttctc
    24121 ttaacctatc aaatggtatt tgtcgtttgt ttgccttatc tataatacta gcgattggtt
    24181 taacacgacc agtaacaaat tctattggcg aggcttgttc accaacttca tattgtttgc
    24241 gcatgccttt aagtttcact tttaactcat caaccgcttg cttataaggt gttaagaact
    24301 gatcccattg attcatagtg cttcaccccg ctttattcaa tgtcgaatgt ttcttcaacg
    24361 gttgcaacaa attccttacc ataattatta tttcctttta tgttgtctaa aatataagta
    24421 agttcttggt ggaattcagt caattctagt tcatcattga taataatttt tttagtcatg
    24481 ttttcatgca acatcgtcca tgtttcttgt acgaaaatga gataagcata cgctttacca
    24541 tcatctaatg tatacgcaaa ggcataatta tcactatcaa cgagcatttg tcctacttca
    24601 gataatcctt taatagaatc ttctgtataa caataaagta tgtcatcttt aattttaatt
    24661 tcattaatat ataaacgcat gcttaatcct cctcttattc cataacattt taacacaatt
    24721 caataataca aaacacttac gaacactatt tttaatggtc aaattaacat tgataataga
    24781 taaaggaaat tacttttaaa attcaacttt gtcattcata atatagttaa gataaattga
    24841 atttgatacg aggtgtatac atatcatggc aacaaatcat gaaatagaat ttaaacaaat
    24901 gataactgca tcaatctata ataaattgca agagaaatat tttaaagaca gtgtgctgtt
    24961 taagcaagtc aattactata ttgatacacc cgatttcaaa ttaaaggaac atcgctctgc
    25021 actacgcata agagttaaag acaatcaata tgaaatgact ttaaagacac cagcaaaggt
    25081 tggtctatta gaatacaact atatcgtcga cattaaacca gagatgaact tgattatttc
    25141 aaacgacaat cttccagatg acattcgtca aattatagtt gaacagtttg gtgtaaagga
    25201 tacaacttta tcaatacttg gtgcattaac gacatatcgc caagaaacta aatataaagg
    25261 tgatttactc gttttagata aaagtgagta tttcgatact acggattatg agttagagtt
    25321 cgaagttaaa gactataatc aaggtttaca aaagttccaa tctttattac acgaactaaa
    25381 cctcgaacat catcaacctt taaataaact tcaacgtttt ttcaagaaaa aagaaacact
    25441 ttca
//
LOCUS       DAHHOJ010000029        25116 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.029, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000029 DAHHOJ010000000
VERSION     DAHHOJ010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25116
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.029"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            42..602
                     /locus_tag="NBH32_001014"
     CDS             42..602
                     /locus_tag="NBH32_001014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1514633.1"
                     /translation="MNKVEVIKCNDDINKMYDALKARSDRDYLFFKLAIHSGMKITEL
                     LTITVKDTKRLIEKGTLSELCKAHYHSLIKIRLPETLSNELLHYIENKSLSNEDVLFQ
                     SLRTNQVLSRQQAYRIIHQAAIKAGIENVGLTTLRKTFAYHAYQKGIPIPVIQKYLGH
                     QSAIETLNFIGLENECEHSIYISLQL"
     gene            621..3023
                     /gene="mnhA2"
                     /locus_tag="NBH32_001015"
     CDS             621..3023
                     /gene="mnhA2"
                     /locus_tag="NBH32_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit A"
                     /protein_id="HCD1514634.1"
                     /translation="MSLVYLLIAVLVIMAMILLTSKRRAMAKYAGYIALTAPVIASIY
                     FLLQVPSVIKQHYLSVSVPWMTSLDINVDLRLDGLSLMFSLIISLIGIAVFFYATQYL
                     SSRKDNLPRFYLYLTLFMFSMLGIVLADNTILMYVFWELTSVSSFLLISYWYNNGDSQ
                     FGAMQSFMITVFGGLALLVGFIMLYIMTGTNNITEILGQADHIKNHALFIPMIIMFLL
                     GAFTKSAQFPFHIWLPRAMAAPTPVSAYLHSATMVKAGIFLLLRFTPLLGLSNMYIYI
                     VTFVGLITMLFGSITALKQWDLKGILAYSTISQLGMIMAMVGIGGGYAQHQQNAIASI
                     YVFVLFGALFHLMNHAIFKCALFMGVGILDHEAGSRDIRILSGMRQLFPKMNLVMTIA
                     ALSMAGVPFLNGFLSKEMFLDALTQTGQLSQFSLISMISIVFVGVIASVFTFTYALYM
                     VKEVFWTKYDSKVFTKKNIHEPWLFSLPSLILMVLVPVIFFVPNIFGKGIIVPALRAV
                     SGGNHQIDQLAPHVSQWHGFNIPLLLTIIIILLGSVLAIKVDWKKVFTGKIRQISVSK
                     SYEMVYRHFEKFATKRFKRVMQDRLNQYIIMTLGIFMIIIGYGYIRIGLPKVHQLHVS
                     EFGALEIILAIVTVTIGISLIFIRQRLTMVILNGVIGFVVTLFFIAMKAPDLALTQLV
                     VETITTILFIVSFSRLPNVPRSNANKKREIIKISVSLLMALIVVSLIFITQQTDGLSS
                     ISDFYLKADKLTGGKNIVNAILGDFRALDTLFEGLVLIITGLGIYTLLNYQDRRGQDE
                     RE"
     gene            3010..3435
                     /gene="mnhB2"
                     /locus_tag="NBH32_001016"
     CDS             3010..3435
                     /gene="mnhB2"
                     /locus_tag="NBH32_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit B"
                     /protein_id="HCD1514635.1"
                     /translation="MKENDVVLRTVTKLVVFILLTFGFYVFFAGHNNPGGGFIGGLIF
                     SSAFILMFLAFNVEEVLESLPIDFRILMIIGALVSSITAIIPMFFGKPFLSQYETTWL
                     LPILGQIHVSTITLFELGILFSVVGVIVTVMLSLSGGRS"
     gene            3432..3776
                     /gene="mnhC2"
                     /locus_tag="NBH32_001017"
     CDS             3432..3776
                     /gene="mnhC2"
                     /locus_tag="NBH32_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit C"
                     /protein_id="HCD1514636.1"
                     /translation="MNLILLLVIGFLVFIGTYMILSINLIRIVIGISIYTHAGNLIIM
                     SMGTYGSSRSEPLITGGNQLFVDPLLQAIVLTAIVIGFGMTAFLLVLVYRTYKVTKED
                     EIEGLRGEDDAK"
     gene            3766..5262
                     /gene="mnhD2"
                     /locus_tag="NBH32_001018"
     CDS             3766..5262
                     /gene="mnhD2"
                     /locus_tag="NBH32_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit D"
                     /protein_id="HCD1514637.1"
                     /translation="MLSNLLILPMLLPFLCALILVFLKNNDRISKYLYLGTMTITTII
                     SLMLLIYVQRHRPITLDFGGWSAPFGIQFLGDSLSLIMVTTASFVITLIMAYGFGRGE
                     HKANRYHLPSFILFLSVGVIGSFLTSDLFNLYVMFEIMLLASFVLITLGQSVEQLRAA
                     IIYVVLNIIGSWLFLLGIGLLYKTVGTLNFSHIAMRLNDMGDNRTVTMISLIFLVAFS
                     AKAALVLFMWLPKAYAVLNTELAALFAALMTKVGAYALIRFFTLLFDQHNDLIHPLLA
                     TMAAITMVIGAIGVIAYKDIKKIAAYQVIISIGFIILGLGTNTFAGINGAIFYLVNDI
                     VVKTLLFFIIGSLVYITGYRQYQYLNGLAKKEPLFGVAFIIMIFAIGGVPPFSGFPGK
                     VLIFQGALQNGNYIGLALMIITSLIAMYSLFRILFYMYFGDKDGEEVNFKKIPLYRKR
                     ILSILVVVVIAIGIAAPVVLNVTSDATELNTSDQLYQKLVNPHLKGED"
     gene            5263..5745
                     /gene="mnhE2"
                     /locus_tag="NBH32_001019"
     CDS             5263..5745
                     /gene="mnhE2"
                     /locus_tag="NBH32_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit E"
                     /protein_id="HCD1514638.1"
                     /translation="MNQIVLNIIIAFLWVLFQDEDHFKFSTFFSGYLIGLIVIYILHR
                     FFSDDFYVRKIWVAIKFLGVYLYQLITSSISTINYILFKTKDMNPGLLSYETRLTSDW
                     AITFLTILIIITPGSTVIRISQDSKKFFIHSIDVSEKEKDSLLRSIKHYEDLILEVSR
                     "
     gene            5742..6044
                     /gene="mnhF2"
                     /locus_tag="NBH32_001020"
     CDS             5742..6044
                     /gene="mnhF2"
                     /locus_tag="NBH32_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000616642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit F"
                     /protein_id="HCD1514639.1"
                     /translation="MIQTITHIMIISSLIIFGIALIICLFRLIKGPTTADRVVTFDTT
                     SAVVMSIVGVLSVLMGTVSFLDSIMLIAIISFVSSVSISRFIGGGHVFNGNNKRNL"
     gene            6019..6456
                     /gene="mnhG2"
                     /locus_tag="NBH32_001021"
     CDS             6019..6456
                     /gene="mnhG2"
                     /locus_tag="NBH32_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh2 subunit G"
                     /protein_id="HCD1514640.1"
                     /translation="MEITKEIFSLIAAVMLLLGSFIALISAIGIVKFQDVFLRSHAAT
                     KSSTLSVLLTLIGVLIYFIVNTGFFSVRLLLSLVFINLTSPVGMHLVARAAYRNGAYM
                     YRKNDDHTHASILLSSNEQNSTEALQLRAKKREEHRKKWYQND"
     gene            6796..8838
                     /locus_tag="NBH32_001022"
     CDS             6796..8838
                     /locus_tag="NBH32_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HCD1514641.1"
                     /translation="MEIFETILIFIAVVILSSFVHTFIPKVPLAFIQIFLGMLLFITP
                     IPVQFNFDSELFMVTMIAPLLFVEGVNVSRVHLRKYIKPVMMMALGLVITTVIGVGLF
                     IHWIWPDLPIGAAFAIAAILCPTDAVAVQAITKGKVLPKGAMTILEGESLLNDAAGII
                     SFKIAVGVLVTGAFSLVDAVQLFLIASIGGAVVGLLIGMALVRFRLTLMRRGYENINM
                     FTIIQLLTPFVTYLIAELFHASGIIAAVVAGLVHGFERDRIMQVRTQLQMSYNHTWNI
                     LGYVLNGFVFSILGFLVPEVIIKIIKTEPHNLIFLIGITIVVALAVYLFRFVWVYVLY
                     PYFYLAISPFQKMMTKNDDDNPTTEKPPKRSLYALIMTLCGVHGTISLAIALTLPYFL
                     AGHHAFTYRNDLLFIASGMVIISLVVAQVLLPLLTKPAPKTVIGNMSFKVARIYILEQ
                     VIDYLNQKSTFETSFKYGNVIKEYHDKLAFLKTVEKDDENSKELERLQKIAFNVETKT
                     LESLVDEGQITNSVLENYMRYAERTQVYRQASLIRRMIVLLRGALLKRRVQTRVNSAS
                     SLSVTDNLMELNKINKLVHYNVVSRLSKETTKDNTLEVGMVCDGYLMRIENLTPSNFF
                     NSASEDTITKIKLNALREQRRILRELIDTDEVSEGTALKLREAINYDEMVIVDSMT"
     gene            complement(9733..10662)
                     /locus_tag="NBH32_001023"
     CDS             complement(9733..10662)
                     /locus_tag="NBH32_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HCD1514642.1"
                     /translation="MKKLVPLLLALLLLVAACGTGGKQSSDKSNGKLKVVTTNSILYD
                     MAKNVGGDNVDIHSIVPVGQDPHEYEVKPKDIKKLTDADVILYNGLNLETGNGWFEKA
                     LEQAGKSLKDKKVIAVSKDVKPIYLNGEEGNKDKQDPHAWLSLDNGIKYVKTIQQTFI
                     DNDKKHKADYEKQGNKYIAQLEKLNNDSKDKFNDIPKEQRAMITSEGAFKYFSKQYGI
                     TPGYIWEINTEKQGTPEQMRQAIEFVKKHKLKHLLVETSVDKKAMESLSEETKKDIFG
                     EVYTDSIGKEGTKGDSYYKMMKSNIDTVHGSMK"
     gene            complement(10659..11495)
                     /locus_tag="NBH32_001024"
     CDS             complement(10659..11495)
                     /locus_tag="NBH32_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1514643.1"
                     /translation="MLEFVEHLFTYQFLNRALITSIIVGIVCGTVGSLIVLRGLSLMG
                     DAMSHAVLPGVALSFLFGIPMFVGALITGMIASIFIGYITSSSKTKPDAAIGISFTAF
                     LASGIIIISLINTTTDLYHILFGNLLAITNSAFLTTIVIGSIVLILIIIFYRPLMIST
                     FDPTFSRMSGLNTTLLHYFVMLLLSLVTVASIQTVGIILVVALLITPASTAFLISKKL
                     YSMMIIASLISVISSIVGLYYSYIYNIPSGATIVLCTFVIYIITLFFTKFTNRKKRGS
                     LT"
     gene            complement(11489..12232)
                     /locus_tag="NBH32_001025"
     CDS             complement(11489..12232)
                     /locus_tag="NBH32_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1514644.1"
                     /translation="MLETKDLNLFLGNKHVLKNISLSIPVRGEIIGIMGPNGAGKSSL
                     IKSLIGEFNATGTKLLYNKPIQQQLQHITYIPQKAHIDLDFPISVEQVILSGCYKEIG
                     WFRRPNKSARDKLKQLLSDLELESLRHRQISELSGGQLQRVLVARALMSESEVYFLDE
                     PFVGIDFSSEKLIMTKIENLKQQGKLILIIHHDLSKAKQYFDRIILLNQTLRYFGDSE
                     EAMSVTRLNETFMSSTDCSDPSQRSNITC"
     gene            12354..12998
                     /locus_tag="NBH32_001026"
     CDS             12354..12998
                     /locus_tag="NBH32_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HCD1514645.1"
                     /translation="MLTEEKEDYLKAILTNNGDKNFVTNKILSQFLNIKPPSVSEMVG
                     RLEKAGYVETKPYKGVRLTEDGLTHTLDIIKRHRLLELFLIEILKYNWEEVHQEAEIL
                     EHRISDLFVERLDSLLNFPETCPHGGVIPRNNEYKEKYITTILNYEPGDIVTIKRVRD
                     KTDLLIYLSSKDISIGNEVEIVSKDEMNKVIIIKRNDNVIIVSYENAMNMFAEK"
     gene            complement(13078..13830)
                     /locus_tag="NBH32_001027"
     CDS             complement(13078..13830)
                     /locus_tag="NBH32_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HCD1514646.1"
                     /translation="MNYLSNFFNTTIQLNIYIIVIVAACYIAIHQYRHKAVLNYLDVI
                     LNYIPVLTHEFGHVLFNKLAGGRAKDLVIVTSPRERQQTLQQGFAITQSRHLAGQWLT
                     TIGGYFMPPIMLLIGLASSHYQIPSFFIFTYLLIFIYFLILTSRKGSPIVVITLISIM
                     LYFILKDENIVEIQLLVTMSYQYILGVLLGEVLQSSWTIAKLTFQRPSPQWDGSALKE
                     LSHVPIFIYSTIWIIFNLYAVNILLKYTHLIN"
     gene            14066..14830
                     /locus_tag="NBH32_001028"
     CDS             14066..14830
                     /locus_tag="NBH32_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HCD1514647.1"
                     /translation="MTVEERSNTAKVDILGVDFDNTTMLQMVENIKTFFANQSTNNLF
                     IVTANPEIVNYATTYQAYLELINQASYIVADGTGVVKASHRLKQPLAHRIPGIELMDE
                     CLKIAHVNHQKVFLLGATNEVVEAAQYALQQRYPNISFAHHHGYIDLEDETVVKRIKL
                     FKPDYIFVGMGFPKQEEWIMTHENQFESTVMMGVGGSLEVFAGAKKRAPYIFRKLNIE
                     WIYRALIDWKRIGRLKSIPIFMYKIAKAKRKIKKAK"
     gene            complement(14891..15685)
                     /gene="tagH"
                     /locus_tag="NBH32_001029"
     CDS             complement(14891..15685)
                     /gene="tagH"
                     /locus_tag="NBH32_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter
                     ATP-binding subunit TagH"
                     /protein_id="HCD1514648.1"
                     /translation="MNVSVNIKNVTKEYRIYRTNKERMKDALIPKHKNKTFFALDDIS
                     LKAYEGDVIGLVGINGSGKSTLSNIIGGSLSPTVGKVDRNGEVSVIAISAGLSGQLTG
                     IENIEFKMLCMGFKRKEIKAMTPKIIEFSELGEFIYQPVKKYSSGMRAKLGFSINITV
                     NPDILVIDEALSVGDQTFAQKCLDKIYEFKEQNKTIFFVSHNLGQVRQFCTKIAWIEG
                     GKLKDYGELDDVLPKYEAFLNDFKKKSKAEQKEFRNKLDESRFVIK"
     gene            16032..16844
                     /gene="tagG"
                     /locus_tag="NBH32_001030"
     CDS             16032..16844
                     /gene="tagG"
                     /locus_tag="NBH32_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acids export ABC transporter permease
                     subunit TagG"
                     /protein_id="HCD1514649.1"
                     /translation="MSAIGTVFKEHVKNFYLIQRLAQFQVKIINHSNYLGVAWELINP
                     VMQIMVYWMVFGLGIRSNAPIHGVPFVYWLLVGISMWFFINQGILEGTKAITQKFNQV
                     SKMNFPLSIIPTYIVTSRFYGHLGLLLLVIIACMFTGIYPSIHIIQLLIYVPFCFFLT
                     ASVTLLTSTLGVLVRDTQMLMQAILRILFYFSPILWLPKNHGISGLIHEMMKYNPVYF
                     IAESYRAAILYHEWYFMDHWKLMLYNFGIVAIFFAIGAYLHMKYRDQFADFL"
     gene            16943..18046
                     /locus_tag="NBH32_001031"
     CDS             16943..18046
                     /locus_tag="NBH32_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1514650.1"
                     /translation="MNVLIKKFYHLVVRILSKMITPQVIDKPHIVFMMTFPEDIKPII
                     KALNNSSYQKTVLTTPKQAPYLSELSDDVDVIEMTNRTLVKQIKALKSAQMIIIDNYY
                     LLLGGYNKTSNQHIVQTWHASGALKNFGLTDHQVDVSDKGMVQQYRKVYQATDFYLVG
                     CEQMSQCFKQSLGATEEQMLYFGLPRINKYYTADRATVKAELKHKYGITNKLALYVPT
                     YREDKADNRAIDKAYFENCLPGYTLINKLHPSIEHSDIDDVSSIDTSTLMLMSDIIIS
                     DYSSLPIEASLLNIPTIFYVYDEEAYDKVRGLNQFYKAIPDSYKVYTEEDLIMTIQEK
                     EHLLSPLFKDWHKYNTDKSLHQLTEYIDKMVTK"
     gene            18043..19104
                     /locus_tag="NBH32_001032"
     CDS             18043..19104
                     /locus_tag="NBH32_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCD1514651.1"
                     /translation="MRLTIIIPTCNNEATIRQLLISIESKEHYRILCIDGGSTDQTIP
                     MIERLQRELKHISLIQLQNASIATCINKGLMDIKMTDPHDSDAFMVINPTSIVLPGKL
                     DSLTAAFKNNDNIDMVIGQRAYNYHGEWKLKSADEFIKDNRIVTLMEQPDLLSMMSFD
                     GKLFSAKFAELQCDETLANTYNHAILVKAMQKATDIHLVSQMIVGDNDIDTHATSNDE
                     DFNRYITEIMKIRQRVMEMLLLPEQRLLYSDMVDRILFNNSLKYYMNEHPAVTHTTIQ
                     LVKDYIMSMQHSDYVSQNMFDIINTVEFIGENWDREIYELWRQTLIQVGINRPTYKKF
                     LIQLKGRKFAHRTKSMLKR"
     gene            19167..19565
                     /gene="tagD"
                     /locus_tag="NBH32_001033"
     CDS             19167..19565
                     /gene="tagD"
                     /locus_tag="NBH32_001033"
                     /EC_number="2.7.7.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate cytidylyltransferase"
                     /protein_id="HCD1514652.1"
                     /translation="MKRVITYGTYDLLHYGHIELLRRAREMGDYLIVALSTDEFNQIK
                     HKKSYYDYEQRKMMLESIRYVDLVIPEKGWGQKEDDVEKFDVDVFVMGHDWEGEFDFL
                     KDKCEVIYLKRTEGISTTKIKQELYGKDAK"
     gene            complement(19682..20977)
                     /gene="pbp4"
                     /locus_tag="NBH32_001034"
     CDS             complement(19682..20977)
                     /gene="pbp4"
                     /locus_tag="NBH32_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein PBP4"
                     /protein_id="HCD1514653.1"
                     /translation="MKNLISIIIILCLTLSIMTPYAQAANSDVTPVQAANQYGYAGLS
                     AAYEPTSAVNVSQTGQLLYQYNIDTKWNPASMTKLMTMYLTLEAVNKGQLSLDETVTM
                     TNKEYIMSTLPELSNTKLYPGQVWTIADLLQITVSNSSNAAALILAKKVSKNTSDFVD
                     LMNNKAKAIGMKNTHFVNPTGAENSRLRTFAPTKYKDQERTVTTARDYAILDLHVIKE
                     TPKILDFTKQLAPTTHAVTYYTFNFSLEGAKMSLPGTDGLKTGSSDTANYNHTITTKR
                     GKFRINQVIMGAGDYKNLGGEKQRNMMGNALMERSFDQYKYVKILSKGEQRINGKKYY
                     VENDLYDVLPSDFSKKDYKLVVEDGKVHADYPREFINKDYGPPTVEVHQPIIQKANTV
                     AKSMWAEHPLFTIIGGACLVAGLALIVHMIINRLFRKRK"
     gene            21398..23125
                     /locus_tag="NBH32_001035"
     CDS             21398..23125
                     /locus_tag="NBH32_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514654.1"
                     /translation="MKRENPLFFLFKKLSWPVGLIVAAITISSLGSLSGLLVPLFTGR
                     IVDKFSVSHINWNLIALFGGIFVINALLSGLGLYLLSKIGEKIIYAIRSVLWEHIIQL
                     KMPFFDKNESGQLMSRLTDDTKVINEFISQKLPNLLPSIVTLVGSLIMLFILDWKMTL
                     LTFITIPIFVLIMIPLGRIMQKISTNTQSEIANFSGLLGRVLTEMRLVKISNTERLEL
                     DNAHKNLNEIYKLGLKQAKIAAVVQPISGIVMLLTIAIILGFGALEIATGAITAGTLI
                     AMIFYVIQLSMPLINLSTLVTDYKKAVGASSRIYEIMQEPIEPTEALEDSENVLIDDG
                     VLSFEHVDFKYDVKKILDDVSFQIPQGQVSAFVGPSGSGKSTIFNLIERMYEIESGDI
                     KYGLESVYDIPLSKWRRKIGYVMQSNSMMSGTIRDNILYGINRHVSDEELINYAKLAN
                     CHDFIMQFDEGYDTLVGERGLKLSGGQRQRIDIARSFVKNPDILLLDEATANLDSESE
                     LKIQEALETLMEGRTTIVIAHRLSTIKKAGQIIFLDKGQVTGKGTHSELMASHAKYKN
                     FVVSQKLTD"
     gene            23484..24713
                     /locus_tag="NBH32_001036"
     CDS             23484..24713
                     /locus_tag="NBH32_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1514655.1"
                     /translation="MFLLINIIGLIVFLGIAVLFSRDRKNIQWQSIGILVVLNLFLAW
                     FFIYFDWGQKAVRGAANGIAWVVQSAHAGTGFAFASLTNVKMMDMAVAALFPILLIVP
                     LFDILMYFNILPKIIGGIGWLLAKVTRQPKFESFFGIEMMFLGNTEALAVSSEQLKRM
                     NEMRVLTIAMMSMSSVSGAIVGAYVQMVPGELVLTAIPLNIVNAIIVSCLLNPVSVEE
                     KEDIIYSLKNNEVERQPFFSFLGDSVLAAGKLVLIIIAFVISFVALADLFDRFINLIT
                     GLIAGWIGIKGSFGLNQILGVFMYPFALLLGLPYDEAWLVAQQMAKKIVTNEFVVMGE
                     ISKDIASYTPHHRAVITTFLISFANFSTIGMIIGTLKGIVDKKTSDFVSKYVPMMLLS
                     GILVSLLTAAFVGLFAW"
     gene            24968..25060
                     /locus_tag="NBH32_001037"
     CDS             24968..25060
                     /locus_tag="NBH32_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514656.1"
                     /translation="MFCYYKQHKGDNFSIEEVKNIIADNEMKVN"
BASE COUNT         8422 a         3611 c         4385 g         8698 t
ORIGIN      
        1 ttaatgttta tgattaagac acttaaagcg aggtgggaca tatgaacaag gtagaagtaa
       61 ttaaatgcaa tgatgatatt aataaaatgt atgacgcact caaagctcga tctgataggg
      121 attatttgtt ttttaaatta gctatacata gtggtatgaa aattacagaa ttattaacaa
      181 ttactgttaa agatactaaa cgactgattg aaaaggggac actaagcgaa ttgtgtaaag
      241 cccattatca ctcattgatt aaaattagat tgccagaaac attatctaat gagttacttc
      301 actatattga aaacaaaagt ctgtcaaatg aagatgtatt atttcaatca ttgagaacta
      361 atcaagtatt atctaggcaa caagcctatc gtattattca tcaagcagca attaaggctg
      421 gcatagagaa tgtaggacta acgacattgc gtaagacatt tgcgtatcat gcatatcaaa
      481 agggcatacc tattcctgtc attcaaaagt atttagggca tcaatctgct attgaaacac
      541 taaattttat cggtttagaa aatgaatgtg aacacagtat ttatatttca ttacaattat
      601 aacgacaaag gaggctaata atgagtttgg tttatctatt aattgccgta ctagtaatta
      661 tggcgatgat attattaaca tctaaacgta gagcaatggc taaatatgca ggatacatag
      721 cactgactgc acctgtaata gcatctattt attttttgtt acaagttcca tcagtaatta
      781 aacagcatta tttgtctgtt tccgttccgt ggatgacctc attagatatt aatgtagatt
      841 tacgtttaga tggtttgagt ttaatgtttt ctcttattat ttcacttatt ggaattgctg
      901 tattcttcta tgcaacacaa tatttatcct ctcgtaaaga taatttacca aggttttact
      961 tatacttaac gctatttatg tttagcatgc tcggtattgt attagctgat aatacaatat
     1021 taatgtatgt gttttgggaa ctaactagcg tatcatcatt tctattaatt tcatattggt
     1081 ataacaacgg agatagtcaa tttggtgcga tgcaatcatt tatgattaca gtatttggtg
     1141 gtttggcatt attagttggt tttatcatgt tgtatatcat gacaggaacg aataacatca
     1201 cagagatatt aggacaagca gatcatatta agaaccatgc tttatttatc cctatgatta
     1261 tcatgttttt attaggtgca tttacaaagt ctgcacaatt cccatttcat atttggttac
     1321 caagggcaat ggcagcgcct acacctgtaa gtgcatattt acattcagcc actatggtta
     1381 aagctggtat ctttttatta cttagattta cacctttgtt aggtctaagc aatatgtata
     1441 tttatatcgt aacctttgtc gggttaataa caatgttatt tggttcaatc acagctttaa
     1501 aacaatggga tttaaaagga atcctagcat attcaacaat cagtcaacta ggaatgatta
     1561 tggcaatggt cggtatcggt ggtggatatg ctcaacacca acaaaacgca atagcatcta
     1621 tttatgtatt tgtattattt ggtgcgctat ttcatctaat gaatcatgcc atctttaaat
     1681 gtgcgctttt catgggagta ggtattttag atcatgaagc aggttcaagg gatatacgaa
     1741 ttttaagtgg aatgcgtcaa ctatttccta aaatgaatct agtcatgacg atagcggctc
     1801 tatctatggc tggagtacca tttttaaatg gatttttaag taaagaaatg ttcttagatg
     1861 cattaacaca aactggacaa ttatcccaat ttagtttgat ttcaatgata agtattgtgt
     1921 ttgttggtgt tattgcgagt gtttttacat tcacatatgc attatacatg gtaaaagaag
     1981 tattttggac aaaatatgat tctaaggttt ttactaaaaa aaatatccac gaaccatggt
     2041 tgtttagttt accatctctt atattaatgg tgctagtacc tgtaatcttt tttgtaccaa
     2101 atatatttgg gaaggggatt atcgttccag cattaagagc tgtatcaggt ggtaatcatc
     2161 aaattgatca attggcacca catgtttcgc aatggcatgg atttaacata ccgcttcttt
     2221 taaccatcat cattatttta ttgggtagtg tactagcaat caaagtagat tggaaaaaag
     2281 tgttcacagg taaaattaga cagatttcag tttcaaaaag ctatgagatg gtatatcgac
     2341 attttgaaaa gtttgctacg aagcgattta aacgtgttat gcaagatcgt ttaaaccaat
     2401 acattattat gaccttaggc atatttatga ttatcattgg atatggttat attcgaattg
     2461 gacttccaaa agtacatcag ttacatgttt ctgaatttgg ggcattagaa attatattag
     2521 caatcgtaac tgtcacaatt ggtatttctt taatttttat acgtcaacga ctgacaatgg
     2581 tcattttaaa tggagtcatc ggatttgttg tgaccttatt ctttatagca atgaaagccc
     2641 ctgatttagc attgactcag ctagtagttg aaacaataac gacgatacta tttattgtta
     2701 gtttttcaag attaccaaac gtgccaagat ctaacgctaa caaaaaaaga gaaataatta
     2761 aaatttctgt atcactcttg atggcactta ttgttgtatc attaattttt attacacaac
     2821 aaacagatgg tttatcatca atatcagact tttatttaaa agctgacaaa ctaacaggtg
     2881 gtaaaaatat tgtaaatgcg atacttggtg actttagagc attagataca ttatttgaag
     2941 gattagtgtt aattattact gggctaggta tttacacatt attaaattat caagatcgga
     3001 ggggacaaga tgaaagagaa tgatgtcgtg ttaagaacgg tcacgaaact tgttgtattt
     3061 attttattga ctttcggatt ctatgtcttc ttcgcaggtc ataataatcc tggtggtggc
     3121 tttattggtg gtttaatatt tagttcagcg tttattttaa tgtttctggc ttttaatgtt
     3181 gaagaggttt tagaaagttt accgattgat tttagaattt taatgattat tggagcattg
     3241 gtatcatcta ttactgcgat aatacctatg ttttttggaa aaccattttt gtctcaatat
     3301 gaaacaactt ggttacttcc aattttaggg caaattcatg taagtacaat aacacttttt
     3361 gaattaggta ttttattctc agttgttggt gttattgtca cagtgatgtt gtcgcttagc
     3421 ggaggtcgat catgaattta atattattac tagttatagg atttttagtg tttataggaa
     3481 catatatgat tttatcaatc aatttaattc gtattgtaat tggaatttca atatatactc
     3541 atgctggtaa tctcattatt atgagtatgg gaacgtatgg ttctagtaga tcagaaccat
     3601 taataactgg tggaaaccaa ttgtttgttg atcccttgtt acaagctatt gtactaactg
     3661 caatagttat agggtttggg atgactgcgt ttttacttgt acttgtttat agaacttata
     3721 aagtaacaaa agaagatgaa attgaaggcc taagggggga agatgatgct aagtaactta
     3781 ttgattttac caatgttatt accattcctt tgtgccttaa tccttgtatt tttaaaaaat
     3841 aatgatcgta tttctaaata tttatactta ggtacaatga ctatcaccac aattatttca
     3901 ttaatgttat taatttatgt tcagcgtcac cgtccaatta cgctagactt tggaggatgg
     3961 tcagcgccct ttggtataca gtttttagga gattctttaa gtttaattat ggttacaacc
     4021 gcttcatttg tgattacttt aattatggca tacggatttg ggcgtggcga acataaagca
     4081 aatcgttatc acttgccatc gttcatatta tttttaagcg ttggcgtgat aggctctttt
     4141 ctaacatcag atttatttaa tttatacgtc atgtttgaaa ttatgttact tgcgtcattt
     4201 gtactcatta cacttggaca atctgttgaa caattacgtg ccgcaattat ttatgttgtc
     4261 ttgaatatta ttggttcatg gctattctta ttaggtatag gtttacttta taaaacagta
     4321 ggtacattaa acttttcaca tattgcaatg cgtttgaatg atatgggaga taatcgcact
     4381 gtaacaatga tttcattaat cttcttagtc gcatttagtg cgaaagcagc gctggttctt
     4441 tttatgtggc tacccaaagc atacgcagtg ttaaatactg agcttgcagc attatttgca
     4501 gcgttaatga ccaaagtagg ggcctatgca ttaattcgat tcttcacttt actattcgat
     4561 caacataatg atctcataca tccattgcta gcaactatgg ctgctataac tatggtcatc
     4621 ggcgctatag gtgtcattgc ttataaagat attaaaaaga ttgcagctta ccaagtcata
     4681 atctcaatag gatttatcat tttaggtttg ggaacaaaca cgtttgcagg tattaatggt
     4741 gcaatatttt atttggtaaa tgacattgtt gtaaaaacat tgctattctt tattattggt
     4801 agtttagttt acattacagg ctatcgacaa tatcaatatt tgaatggctt agctaaaaaa
     4861 gaacctttat ttggagttgc gtttattata atgatttttg ctattggcgg cgtgccccca
     4921 tttagtggct ttccggggaa agtacttatt ttccaaggcg cattgcaaaa tggcaattat
     4981 attggactag cgttaatgat tattactagt ctaattgcaa tgtacagttt atttaggata
     5041 cttttttata tgtattttgg agataaagat ggggaggaag ttaattttaa gaaaatccca
     5101 ctatatcgaa aaagaatttt aagtatttta gtagttgtgg ttatcgcaat cggaattgct
     5161 gcgcctgtag tgttaaatgt tacaagtgat gcaactgagt tgaacacgag tgatcaatta
     5221 tatcaaaaac ttgtaaatcc gcatttgaaa ggagaggact aaatgaatca aatagtttta
     5281 aatattatca ttgcattctt atgggtatta tttcaagatg aagatcattt taaattctcg
     5341 actttctttt ctggatatct aattggtcta attgtcattt atatattaca caggtttttc
     5401 agcgatgatt tttatgttag aaaaatttgg gtagctatta aatttttagg tgtttattta
     5461 tatcaattaa taacatctag cattagcacg attaattata ttctttttaa aacaaaagat
     5521 atgaaccctg gattactttc atatgaaaca agactaacaa gtgactgggc aataacattt
     5581 ttaacaattt taattattat aactccaggg tctacagtaa tacgaatttc tcaagactct
     5641 aaaaagtttt ttattcatag tatcgacgtg tcagaaaaag aaaaagatag tttgttaaga
     5701 agtattaagc attatgaaga cttaatattg gaggtgtcgc gatgatacaa acaataacac
     5761 atattatgat tattagttca ctcattattt ttggaattgc attaatcatc tgtttattta
     5821 gattaatcaa gggacctaca acagcagatc gtgtcgttac atttgataca acaagtgctg
     5881 tcgtaatgtc aattgtgggt gtgttaagtg tacttatggg caccgtttct ttcttagatt
     5941 caatcatgct cattgccatt atatcttttg taagttctgt ttcaatatca cgctttattg
     6001 gtggggggca tgtgtttaat ggaaataaca aaagaaatct ttagtcttat tgctgctgtg
     6061 atgttgttgt taggtagttt tattgctctt attagtgcaa taggtatcgt gaaattccaa
     6121 gatgttttct taagaagtca cgctgcgaca aaaagttcaa ctttatcagt gttattaact
     6181 ttaatcggtg tattaattta ttttattgtg aatacaggat ttttcagtgt gcgtttatta
     6241 ctgtcacttg tttttattaa tttaacttca cccgtcggca tgcacttagt cgctcgcgct
     6301 gcttatcgca acggcgctta tatgtatcga aaaaatgatg atcacacaca tgcatcaata
     6361 ttattaagtt caaatgaaca aaactctaca gaagcattac aattacgtgc taaaaaacga
     6421 gaagagcatc gtaagaaatg gtatcaaaac gattgaagtg ttggctaaat tgttaagcat
     6481 aacgatgctt ttgaagattt gttttcaaaa ttaatcatta aaagagtact ttgaaattgg
     6541 ttagtcgatt ttaaggtgct cattttttgt attataagtt aatgttgtta tgtgaaagca
     6601 agttgtttat ttatacaaac aattagtgat aaaataaaat taaataattg caaatttaag
     6661 tactgcaatc gtagacatat atctatatta aagtgctttt gtggtcgcta actcaattta
     6721 tcctctaaag tataattaca acgagatgtg atacttaatt ttcaattaaa caatactttt
     6781 tagagaggtg aaagtttgga aatatttgaa acaattctta tatttatagc tgttgtgata
     6841 ctaagttcgt ttgtccatac tttcatacct aaagtacccc tagcatttat acaaattttc
     6901 ttgggcatgt tactatttat taccccaatc cctgttcaat ttaattttga ttctgaattg
     6961 tttatggtaa caatgattgc gcctttgtta tttgtagaag gtgttaatgt ttctagagtc
     7021 catttaagaa aatatattaa gccagtgatg atgatggcat taggattagt cattactact
     7081 gtgataggtg taggtttatt tattcattgg atttggccag atttacctat tggagcagca
     7141 tttgcaattg ctgccattct ttgccctact gatgcagtag cagtgcaagc aatcactaaa
     7201 ggaaaggtct tgccaaaagg agcaatgaca attcttgaag gtgagtcatt attgaatgat
     7261 gctgctggta ttatttcatt taaaatagct gttggagtat tagttacagg tgctttttca
     7321 cttgttgatg ctgttcagtt gtttttaatt gcatcaattg gtggcgcagt ggttggttta
     7381 cttataggta tggcattagt aaggttccga ttaacattga tgcgtcgagg atatgaaaac
     7441 attaatatgt ttacaattat tcaattgtta acaccatttg ttacgtattt aattgctgaa
     7501 ttgtttcacg catcaggaat cattgcagca gtagttgcag gacttgtaca tggtttcgaa
     7561 cgtgacagaa ttatgcaagt acgtacacaa ctacaaatga gttacaatca tacatggaat
     7621 atactaggtt atgttttaaa tggctttgtt ttttcaatat taggattttt agtacctgaa
     7681 gttattatta aaattatcaa aacagaaccg cacaatttaa tctttttaat aggcatcact
     7741 attgttgttg ctttagctgt ctatctattt agatttgttt gggtttatgt cttatatcct
     7801 tatttttatt tagccatcag tccattccaa aaaatgatga ctaaaaatga tgatgataat
     7861 ccaacgactg agaaaccacc aaagcgaagt ttatacgctt taattatgac gttatgtggt
     7921 gtgcatggaa caatttcttt agcaatcgca ttaacgttac cgtatttttt agcagggcat
     7981 catgctttta cgtatagaaa cgacttatta tttattgcat ctggtatggt tattattagt
     8041 ttggtagttg cgcaagtatt attgccacta ttaacgaaac ctgcacctaa aacagtaatt
     8101 ggcaatatgt cgtttaaagt tgctagaatt tatatattag aacaagttat tgattatcta
     8161 aatcaaaaat ctactttcga aacaagtttt aaatatggta acgtgattaa agaatatcat
     8221 gataaattag catttttaaa aactgtagag aaagatgatg aaaactctaa agaattagaa
     8281 cgtctacaaa aaattgcttt taatgtagaa acaaaaacat tagagtcttt agtagatgaa
     8341 ggacaaataa cgaatagtgt acttgaaaac tatatgcgtt atgctgaaag aacacaagta
     8401 tatagacaag catcattaat aagaagaatg attgtattat tacgaggtgc tttattaaaa
     8461 cgaagagtac aaacgagagt gaactcggca tcttcactta gtgttacgga taacttaatg
     8521 gaattaaata aaattaataa attagtccat tataatgtgg ttagtcgatt gtctaaggaa
     8581 acaacaaaag ataatacact tgaagttgga atggtttgtg acggttattt aatgcgaatt
     8641 gaaaacttaa caccatctaa tttcttcaac tcagcaagtg aagatacgat tactaaaatt
     8701 aaattaaatg cattgagaga acaacgtcgc attttacgtg agttgattga tacagatgaa
     8761 gtatcagaag gtacagcgtt aaaactaaga gaggccatta attatgatga aatggttatt
     8821 gtagatagta tgacgtagtt cgtaattatg ctaaaaggga ttgatgaaaa aatgaatggc
     8881 ttttcatcaa tcccttttat tttaggggaa ttgaatagat agttttaaac tatacgaatt
     8941 attaatattt gagaattaat tgaaataagt tttaaaaatt ggaggagata gattaatcga
     9001 agtcatttaa attaagaaga tacgtgcatt cacaaaaaat agccacactc atatgacatc
     9061 ggatgagtgt ggcttaagga tctatggggg gaggaaacca tagatgttta ctttgatagg
     9121 ccagattaaa tatcaaagta tgcgattatt tatagcttga tgcaaaagtg gtatgcctat
     9181 taaaagttac tgcacatagc ttttaatatt ccgttcaaag gaaaggggca tacaattgaa
     9241 caatctgtaa tagtactttt aaccagctat gctaaaagtc tagtagggag aacagttgtc
     9301 caatcacata agaacctcta acttcgttag tacgattaag aaaagctttt tagttagtat
     9361 gtaatacaat ttattgacgc gcgtgaatct cttttataag agtgtgtagg gaatggcgtt
     9421 gtataaattg tattagaaga acttctaacg catctctgtg gttaaaagag atgaagggaa
     9481 cgacagttta attaaaactg cataagaact tctagctttt ctctttcgtt caaagagaag
     9541 ttctaatacc accatatcgt gcgatcggga acggtatata tattaatagg agggtaatat
     9601 atatttaacg cacgatatgg gactattagc cttcgacttt gttatgttga tgtgtggcct
     9661 aaaatattgg agataccaat attttaggtt gcatcaacat caattcatct tacttcatta
     9721 aaacacagcg tgttatttca tgcttccgtg tacagtatca atatttgatt tcatcatttt
     9781 gtagtaagag tcacctttag tgccttcttt accgattgaa tctgtgtaca cttcaccaaa
     9841 gatatctttc ttcgtttctt cagataaact ttccattgct ttcttatcaa cacttgtttc
     9901 tactaataag tgttttaatt tgtgcttttt aacaaactca atagcttgtc tcatttgttc
     9961 aggtgtacct tgtttttcag tgttaatttc ccaaatataa cctggtgtaa taccgtattg
    10021 ttttgagaag tacttgaagg caccttcact tgtaatcatg gcacgttgtt cttttggaat
    10081 gtcattaaat ttgtctttac tgtcattatt taatttttcc aattgagcaa tgtatttgtt
    10141 accttgcttt tcataatctg ctttatgttt tttgtcgtta tcgataaatg tttgttgaat
    10201 tgtttttacg tatttaatac cgttatctaa acttaaccat gcgtgtggat cttgtttatc
    10261 tttgttgcct tcttcaccgt ttaaatagat aggtttaaca tcttttgata ctgcgataac
    10321 ttttttatct tttaatgatt taccagcctg ttctaaggct ttttcaaacc aaccgttacc
    10381 agtctctaaa tttaatccgt tgtataaaat aacgtcagcg tcagttaact ttttaatatc
    10441 tttaggttta acttcatatt catgaggatc ttgaccaaca ggtacaatac tatgaatatc
    10501 gacgttgtct ccaccaacat ttttagccat atcatataaa attgaattcg tcgttactac
    10561 ttttaatttg ccatttgact tatcactgct ttgtttacca ccagtaccac atgcagcaac
    10621 tagaagtaat aaggctaata ataaaggtac taattttttc atgttaaact tcctcgtttc
    10681 tttctattcg taaattttgt gaaaaataat gtgatgatat aaattacaaa cgtacaaagt
    10741 acgattgtcg caccactagg aatgttgtaa atatagctgt aataaagtcc gacaattgaa
    10801 cttatgacac ttattaaact tgctataatc atcattgagt atagtttttt actaattaaa
    10861 aatgctgtag atgcaggtgt aattaataat gcaactacaa gaataatacc taccgtttga
    10921 atacttgcta ctgttactaa tgagagtaac aacatcacaa agtaatgtaa taacgtcgta
    10981 tttagaccac tcattctact aaacgttgga tcgaatgtag aaatcattaa tggacgatag
    11041 aaaataatga ttagaataag gacgattgaa ccaatcacaa tagttgttaa aaatgcacta
    11101 tttgtgattg ccagtaaatt accaaacaga atatggtaca aatctgtcgt agtgtttatt
    11161 aagctaataa taataatccc cgaagctaag aaagcggtaa aactaattcc aatagcggcg
    11221 tcaggtttcg ttttactact agatgtgata taaccgataa aaatacttgc gatcatacca
    11281 gttataagtg cgcctacaaa cattggaata ccaaataaga atgatagggc aacaccaggt
    11341 aatactgcgt gactcattgc atctcccatt aatgaaagac cacgtaatac aattaaacta
    11401 ccaactgtac cacaaactat ccctacaata attgaagtta tcaatgctcg attcaagaat
    11461 tgatatgtaa ataaatgttc gacaaactct aacatgttat attgctcctt tgactagggt
    11521 cactacagtc agtgctactc ataaatgttt cgtttaagcg agtgacactc atagcctctt
    11581 cactatcacc gaagtatcgt aatgtttgat ttaatagaat aatgcgatca aagtattgct
    11641 ttgcttttga tagatcatgg tggatgataa gaataagttt tccttgttgt tttaagttct
    11701 cgatttttgt catgattaat ttttcgctac taaaatcaat tccgacaaac ggctcatcta
    11761 gaaaataaac ttcactttcg gacatcaatg ctcttgctac tagcacacgt tgtaattgtc
    11821 caccacttaa ttctgaaatt tgtcgatgac gtaaagattc taattctaaa tcgcttaata
    11881 actgtttgag tttatccctt gctgatttat taggtcgtct aaaccatcca atttctttgt
    11941 agcaacctga taaaatcact tgttccacac ttataggaaa atctaaatca atatgtgctt
    12001 tttgtggaat atatgtaata tgttgcagtt gttgttgtat aggtttgtta tataacaatt
    12061 tagtaccggt agcattaaat tcaccaatta aagacttgat aagggaagat ttaccagcac
    12121 cattcgggcc catgatacca attatttcgc cgcgtactgg tatcgataag gaaatgtttt
    12181 taagtacatg cttattacct aaaaacagat ttaaatcttt tgtttctaac aaacgtttat
    12241 acctcctaat taaaagttta ggctaaccta attaattgta taataaactg agaatattta
    12301 tcatgtcaag taaattcgtg atataatata gacaatgtat gtgaggtgaa agtatgttaa
    12361 ctgaagaaaa agaggactat ttaaaggcaa tccttacgaa taatggcgat aaaaactttg
    12421 tgacaaataa aatcttatct caatttttaa atattaagcc tccatctgta agtgaaatgg
    12481 taggacgtct tgaaaaagca ggctatgttg aaacaaaacc atacaaaggt gttagattaa
    12541 cagaggatgg tttaacgcat acgcttgata tcattaagag acatcgacta ttagaattat
    12601 ttttaataga aatattgaaa tataattggg aagaagtaca tcaagaagca gaaattttag
    12661 aacatcgaat ttcagattta tttgttgaaa ggctggatag cctgttaaat ttcccagaaa
    12721 cttgcccgca cggcggtgtg attcctagaa ataatgaata taaagagaaa tatataacaa
    12781 cgattttgaa ttatgaacct ggtgatatcg ttacaatcaa acgtgtgaga gataagaccg
    12841 atttgctaat atatttgtct agtaaagata tttctattgg taatgaagtg gaaattgtat
    12901 cgaaagatga aatgaataaa gtaattatta ttaaacgtaa tgataatgta attattgtca
    12961 gttacgaaaa tgcaatgaac atgtttgctg aaaaataaaa taaagaagcc ataaagatat
    13021 ccatgattgt actgataaag acatatggat aattgcttta ggcttctttt ttattagtta
    13081 atttatcaag tgagtatatt tgagtaaaat attcactgca taaagattga agataatcca
    13141 gattgtacta taaatgaaga taggtacatg actgagttct ttaagtgcac taccatccca
    13201 ctgtggactc ggacgctgga aagtcaattt agcaatcgtc caactagatt gtagaacttc
    13261 gcctaataat acacctaaaa tatattgata actcattgtg acaagtagtt gaatttctac
    13321 tatattttca tcttttaata taaaatacaa catgatagaa attaaagtta taacaacaat
    13381 gggtgagcct tttctagatg ttaaaattaa aaaataaata aatatcaata agtaggtaaa
    13441 tataaagaaa ctaggtatct gataatggct cgacgctaaa cctatcaata acataatagg
    13501 tggcataaaa taaccaccaa tcgttgtaag ccattggcct gctagatgtc tagattgtgt
    13561 aattgcgaat ccttgttgta atgtctgttg tcgctctcgt ggacttgtta caatgactaa
    13621 atcttttgca cggccaccag cgagtttatt aaacaataca tgaccaaatt catgtgttaa
    13681 aacagggata tagtttaaaa tgacatctaa atagttcaaa acagccttat gtctatattg
    13741 atgaatagca atataacaag ctgcaacaat aacgataatg tatatattaa gttgaattgt
    13801 cgtattaaaa aagtttgata aataattcat tgttaacctc atataagata ttaatttaaa
    13861 gtttgcttat cacttattat aaatgatatt ggcattaata gcgttagact ttagacttac
    13921 cttagttaaa ctaattttaa tttttgaaaa ggtgaatatg tgttaaaata aagcaaaatc
    13981 atttcgatat aaataggata aatataaata ctgttaatat tgattacact aacataataa
    14041 tgaaataaga taggagattc ctgttatgac tgttgaagaa agatccaata cagccaaagt
    14101 tgacatttta ggggtcgatt ttgataatac aacaatgttg caaatggttg aaaatattaa
    14161 aacctttttt gcaaatcaat caacgaataa tctttttata gtaacagcca accctgaaat
    14221 agtgaattac gcgacgacat atcaagcgta tttagagtta ataaatcaag cgagctatat
    14281 tgttgctgat gggacaggag tagtcaaagc ttcgcatcgt ttaaagcaac ctctagcgca
    14341 tcgtatacct ggtattgagt tgatggatga atgtttgaaa attgctcatg taaatcatca
    14401 aaaagtattt ttgctagggg caactaatga agttgtagaa gcggcacaat atgcattaca
    14461 acaaagatat ccaaacatat cgtttgcaca tcatcacggt tatattgatt tggaagatga
    14521 gacagtagtg aaacgaatta aactgtttaa acctgattac atatttgtag gtatgggatt
    14581 ccctaaacaa gaagaatgga ttatgacaca tgaaaaccaa tttgaatcta cagtgatgat
    14641 gggcgtaggt ggttctcttg aagtatttgc tggggctaaa aagagagcgc cctatatctt
    14701 tagaaaatta aacattgaat ggatatatag agcattaata gattggaaac gtattggtag
    14761 attaaagagt attcctatat ttatgtataa aatagccaaa gcaaaaagaa aaataaaaaa
    14821 ggcgaaataa tcatgatgac aaaaataaaa ccgaggaaat ccttaaatgg agattctcgg
    14881 ttttttcggt ttatttaata acgaagcggg actcatcgag tttgtttcta aattcttttt
    14941 gttcggcttt ggatttcttt ttaaaatcgt ttaggaaagc ttcatattta ggtaatacat
    15001 catcaagttc accgtaatct tttaactttc cgccttcaat ccaagcaatc ttagtacaaa
    15061 attgtctcac ttggcctaag ttatgactaa cgaaaaagat ggttttgttt tgctctttaa
    15121 actcgtaaat tttatctaaa catttttgtg caaaagtttg gtcacctaca gataaagctt
    15181 cgtcaatgac taagatatct ggattaactg tgatattaat tgaaaaacca agttttgcac
    15241 gcataccact tgaatacttt ttaactggtt gataaataaa ctcaccaagt tcactaaatt
    15301 caataatctt aggtgtcatc gctttaattt cttttcgctt aaagcccata cataacattt
    15361 taaattcgat attttcaatc cctgtaagtt gtccactcaa gccagcacta attgcgataa
    15421 cgctgacttc accattacga tccactttgc caacagtagg cgacaaagaa ccgccaatga
    15481 tattgctcaa cgttgatttg ccggaaccat tgatgccaac aagccctatg acgtcacctt
    15541 catatgcttt taaactaatg tcatctaaag cgaaaaatgt tttgttttta tgtttgggaa
    15601 tgagcgcatc tttcatacgt tctttatttg tacgataaat acgatattct tttgttacat
    15661 ttttaatgtt taccgaaacg ttcatttgta gaccttcctt attcacattt atctagatta
    15721 taatatacta ctcaacagtt gttaaatttt aaaacctgtt gtaaagtgta tagaagattt
    15781 tgttattatc agagttggtg ttttgacaca aaatgttaat catcaatgat aacaatgata
    15841 tttaaaaact aaacttattt caacttacat gattgtatac tataatgtat ttgtaataaa
    15901 ctaatatttt aaagaactag acaataattt tgatagcatc catgtatagt gatagtattt
    15961 acaacaatta ttataatact atttagttaa gtagagaaat agttaaacat ttgaaagtgt
    16021 ggtttaatgg aatgtcagca ataggaacag tttttaaaga acatgtaaag aacttttatt
    16081 taattcaaag actggctcag tttcaagtta aaattattaa tcatagtaac tatttaggtg
    16141 tggcttggga attaattaac cctgttatgc aaattatggt ttactggatg gtttttggat
    16201 taggaataag aagtaatgca ccaattcatg gtgtaccttt tgtttattgg ttattggttg
    16261 gtatcagtat gtggttcttc atcaaccaag gtattttaga aggtactaaa gcaattacac
    16321 aaaagtttaa tcaagtatcg aaaatgaact tcccgttatc gataataccg acatatattg
    16381 tgacaagtag attttatgga catttaggct tacttttact tgtgataatt gcatgtatgt
    16441 ttactggtat ttatccatca atacatatca ttcaattatt gatatatgta ccgttttgtt
    16501 ttttcttaac tgcctcggtg acgttattaa catcaacact cggtgtgtta gttagagata
    16561 cacaaatgtt aatgcaagca atattaagaa tattatttta cttttcacca attttgtggc
    16621 taccaaagaa ccatggtatc agtggtttaa ttcatgaaat gatgaaatat aatccagttt
    16681 actttattgc tgaatcatac cgtgcagcaa ttttatatca cgaatggtat ttcatggatc
    16741 attggaaatt aatgttatac aatttcggta ttgttgccat tttctttgca attggtgcgt
    16801 acttacacat gaaatataga gatcaatttg cagatttctt gtaatatatt tatatgacga
    16861 aaccccgcta accattaata aatggaagtg gggttcattt ttgtttataa tttaagtaaa
    16921 taacatatta agttggtgta ttatgaacgt tttaataaag aaattttatc atttggtagt
    16981 tcgaatactt tctaaaatga ttacgcctca agtgattgat aaaccgcata tcgtatttat
    17041 gatgactttt ccagaagata ttaagcctat catcaaagca ttaaataatt cgtcgtatca
    17101 gaaaacggtt ttaacaacac caaaacaagc gccttattta tctgaactta gcgacgatgt
    17161 tgatgtgata gaaatgacta atcgaacatt ggtaaaacaa attaaggctt tgaaaagcgc
    17221 gcagatgatt attatcgata attattacct attactaggt ggatataata agacttctaa
    17281 tcaacacatt gttcaaacgt ggcatgcaag tggtgcatta aaaaactttg gattaacaga
    17341 tcatcaagtc gatgtgtctg acaagggaat ggttcagcag taccgtaaag tttatcaagc
    17401 gacggatttt tacttagtgg gttgtgaaca aatgtcacaa tgttttaaac agtctttagg
    17461 tgcaacagaa gagcaaatgt tgtattttgg gcttccgaga attaataaat attacacagc
    17521 tgatagagca acggttaagg cagagttaaa gcataaatat ggaattacaa ataagttggc
    17581 attatatgta ccaacatata gagaagataa agcagataat agggctattg ataaagctta
    17641 ttttgaaaac tgtttaccag gatatacact gattaataaa ttacatccat caattgaaca
    17701 ttcagacatt gatgacgtat cttcaatcga cacgtctaca ttaatgctaa tgtcagatat
    17761 aattattagc gactatagtt cgctgccaat agaagctagc ttgttaaata ttccaactat
    17821 attttatgtg tatgatgaag aggcatatga taaagtgaga ggcctgaatc aattttacaa
    17881 agcaataccg gatagctaca aagtgtatac tgaagaagat ttaataatga cgatacaaga
    17941 aaaagaacat ctattaagtc cgttatttaa agattggcat aagtataata ctgataaaag
    18001 tttacatcag ctcacagaat atatagataa gatggtgaca aaatgaggct tacgataatc
    18061 atacctacat gtaataatga ggcaacaatt cgacaattgt taatatctat tgagagtaaa
    18121 gaacactata gaatcctttg tattgatgga ggttctaccg atcaaacaat tcctatgatt
    18181 gaacggttac aaagagaact caagcatatt tcattaatac aattacaaaa tgcttcgata
    18241 gctacgtgta ttaataaagg tttgatggat atcaaaatga cagatccaca tgatagtgac
    18301 gcatttatgg tcataaatcc aacatcaatc gtattgccag gtaaattaga tagtctaact
    18361 gcagctttca aaaataatga taatattgat atggtaatag ggcagcgagc ttacaattac
    18421 catggtgaat ggaaattgaa aagtgctgat gagtttatta aagacaatcg aatcgttaca
    18481 ttaatggaac aaccagattt gttatcaatg atgtcttttg acggaaagtt attcagtgct
    18541 aaatttgctg aattacagtg tgacgaaact ttagctaaca catacaatca cgcaatactt
    18601 gtcaaggcga tgcaaaaagc tacggatata catttagttt cacagatgat tgtcggagat
    18661 aacgatatag atacacatgc tacaagtaac gatgaagatt ttaatagata tatcacagaa
    18721 attatgaaaa taagacaacg agtcatggaa atgttactat tacctgaaca aaggctatta
    18781 tatagtgata tggttgatcg tattttattc aataattcat taaaatatta tatgaatgaa
    18841 cacccagcag taacgcacac gacaattcaa ctcgtaaaag actatattat gtctatgcag
    18901 cattctgatt atgtatcgca aaacatgttt gacattataa atacagttga atttattggt
    18961 gagaattggg atagagaaat atacgaattg tggcgacaaa cattaattca agtgggcatt
    19021 aataggccga cttataaaaa attcttgata caacttaaag ggagaaagtt tgcacatcga
    19081 acaaaatcaa tgttaaaacg ataacgtgta cattgatgac cataaactgc aatcctatga
    19141 tgtgacaata tgaggaggat aacttaatga aacgtgtaat aacatatggc acatatgact
    19201 tacttcacta tggtcatatc gaattgcttc gtcgtgcaag agagatgggc gattatttaa
    19261 tagtagcatt atcaacagat gaatttaatc aaattaaaca taaaaaatct tattatgatt
    19321 atgaacaacg aaaaatgatg cttgaatcaa tacgctatgt cgatttagtc attccagaaa
    19381 agggctgggg acaaaaagaa gacgatgtcg aaaaatttga tgtagatgtt tttgttatgg
    19441 gacatgactg ggaaggtgaa ttcgacttct taaaggataa atgtgaagtc atttatttaa
    19501 aacgtacaga aggcatttcg acgactaaaa tcaaacaaga attatatggt aaagatgcta
    19561 aataaattat atagaactat cgatactaaa cgataaatta acgtaggtta ttataaaata
    19621 aatataaaac ggacaagttt cgcagcttta taatgtgcaa cttgtccgtt tttagtatgt
    19681 tttattttct ttttctaaat aaacgattga ttatcatatg aacaattagt gctaatccag
    19741 cgactaggca tgcaccacca atgatagtga ataatggatg ttctgcccac atacttttag
    19801 caacagtatt tgccttttga ataattggct gatgaacttc tacagttgga ggtccataat
    19861 ctttattaat aaattctctt ggatagtccg cgtgtacttt accatcttcg actacaagtt
    19921 tataatcttt tttactaaaa tcacttggta aaacatcgta aagatcgttt tcaacataat
    19981 atttcttacc atttatcctt tgctcacctt tagacaatat ttttacatat ttatactgat
    20041 caaatgagcg ttccattaat gcattcccca tcatattacg ttgcttctcg ccaccaaggt
    20101 ttttatagtc tcccgcaccc atgataactt gattaattct aaatttacct cgtttggtag
    20161 taatcgtatg gttgtaattt gctgtatcac ttgatccagt ttttaaacca tctgtaccag
    20221 gcaaactcat ttttgcacct tccaatgaaa agttgaatgt gtaatacgta actgcatgcg
    20281 ttgttggtgc taactgcttt gtaaagtcta atattttagg tgtctcttta atcacgtgta
    20341 aatctaaaat ggcatagtct ctagcagtcg ttacagtacg ttcttggtct ttatactttg
    20401 ttggtgcaaa tgtacgtaat cttgaatttt cagcacccgt tggattgacg aaatgtgtat
    20461 ttttcattcc gatagcttta gctttgttat tcattaaatc aacgaaatcg ctggtgtttt
    20521 ttgatacctt cttagctaaa attaatgccg cggcattact agaattagat actgtaattt
    20581 gtaataggtc tgcgattgtc catacttgtc caggatatag tttcgtatta ctcaactcag
    20641 gtagtgtaga cataatatat tctttgttcg tcattgtgac agtttcatca agtgaaagct
    20701 gccccttatt tacagcttcc aatgttaagt acattgtcat taatttagtc atagacgctg
    20761 gattccactt agtatcgata ttgtattgat acagtaattg tccagtttga ctaacattaa
    20821 cagcactcgt cggttcgtat gcagccgaca aacctgcata accatattga tttgctgctt
    20881 gtacaggggt tacgtcactg ttagcagctt gtgcatatgg tgtcataata cttaatgtta
    20941 aacataaaat gatgataata gatattaaat ttttcataaa gcgttaatct tcccttttcc
    21001 aattcttaaa tattccctaa aagcaatggt cattcctaat tacggaaatc attgctaatt
    21061 cacttcacct taattaaatt gttgaaaatt aagtttcttg cggtttattt gaaaaataat
    21121 gcaaatatat tacgtgtgta gctaaaggtg ttataatgtt tgtacgaaga gcaaacttac
    21181 tcaaaagcga ttaattatca tgttttaata taaaaacttt gagaagttat tacaaaaaat
    21241 gcaatagaaa tattctatca tataaatgtt atgaacggta ttttggggca atactttatt
    21301 tgatttttaa agttttgttg ggagaaagta tatgatagaa atgcatgtat ctatctaaat
    21361 gaattaacta taaatttcaa acaaaagagg taaaactatg aaacgagaaa atccattgtt
    21421 tttcttattt aaaaaactat catggccagt gggtcttatc gttgcagcta tcactatttc
    21481 atcactaggg agcttaagtg gactattagt gccactgttt actggacgaa ttgtagataa
    21541 attttccgtg agccatatca attggaatct aatcgcatta tttggtggta tctttgtcat
    21601 caatgcttta ttaagcggat taggtttata tttattaagt aaaattggtg aaaaaattat
    21661 ttacgcgata cgctcagttt tatgggagca tatcatacaa ttaaaaatgc cattctttga
    21721 taaaaatgaa agtggtcaat taatgagtcg attaactgac gatacgaaag tgataaatga
    21781 atttatttca caaaagctac ccaacttatt accatcaatc gttacattag ttgggtcact
    21841 aatcatgtta tttattttag attggaaaat gacattatta acatttataa cgataccgat
    21901 atttgtttta attatgattc ctctaggacg tattatgcaa aaaatatcga caaatacaca
    21961 atctgaaatt gcaaacttta gtggtttgtt agggcgtgtc ctaactgaaa tgcgtcttgt
    22021 taaaatatca aatacagagc gacttgaatt agataatgca cataaaaatt tgaatgaaat
    22081 atataaatta ggtttgaaac aggctaaaat tgcggcagtt gtacaaccaa tttcaggtat
    22141 tgttatgttg ctaacaattg caattatttt aggttttggt gcattagaaa ttgcgactgg
    22201 tgcaatcact gcaggtacat taattgcaat gatattttat gttattcaat tatcgatgcc
    22261 tttaatcaat ctttcaacgt tagttacaga ctataaaaag gcagtcggtg caagtagtag
    22321 aatatacgaa atcatgcaag aacctattga accgacagaa gctcttgaag attctgaaaa
    22381 tgtattaatt gatgacggtg tgttgtcatt tgaacatgta gactttaaat atgatgtgaa
    22441 gaaaatatta gatgatgtgt cgttccaaat cccacaaggg caagtgagtg cttttgtagg
    22501 tccttctgga tctggtaaaa gtacgatatt taatctgata gaacgtatgt atgaaattga
    22561 gtcaggtgat attaaatatg gccttgaaag tgtctatgat atcccgttat ctaagtggcg
    22621 acgcaaaatt ggatatgtta tgcaatcaaa ttcgatgatg agtggtacaa ttagagacaa
    22681 tattttatac ggaattaatc gtcatgtttc agatgaagaa cttattaatt atgctaaatt
    22741 agcgaactgt catgatttta tcatgcaatt tgatgaagga tatgacacgc ttgtaggtga
    22801 acgaggattg aaactgtctg gcggacaacg tcaacgtatt gatattgcta gaagttttgt
    22861 taaaaatccc gatattttgt tacttgatga agcaacagct aatctcgata gtgaaagtga
    22921 attgaaaatt caagaagctt tagaaacatt gatggaaggt agaacaacga ttgtcattgc
    22981 gcatcgtttg tctacaatta aaaaagccgg tcaaattata ttcttagaca aaggacaggt
    23041 aacaggtaaa ggtacgcatt cagaactaat ggcatcacat gcgaagtata aaaactttgt
    23101 agtgtctcaa aaattaacag attaatttta tatatataag taagcttgga gcaagtacag
    23161 atataccatc gaggaaatta aagcgtggca cattgatgga tatagatgtt aataaattgc
    23221 ttcaagcttt tgtctatttt aaatcatttg agaagttacg acataataat tcttaaatta
    23281 atgaaatcga tattttaaga aaaaaatgct catggtataa tacaagttat aagcaaacat
    23341 acatatatta aatactgtag ccacgagtca taattcttca tattttacat agcaatttaa
    23401 ctgattttag agtccacggt acagaagttt gatatttcaa tgtttctaaa tttttaaaaa
    23461 attaaatcat aggtgggtgc caaatgtttt tattaatcaa cattattggt ctaattgtat
    23521 ttcttggtat tgcggtatta ttttcaagag atcgcaaaaa tatccaatgg caatcaattg
    23581 ggatcttagt tgttttaaac ctgtttttag catggttctt tatttatttt gattggggtc
    23641 aaaaagcagt aagaggagca gccaatggta tcgcttgggt agttcagtca gcgcatgctg
    23701 gtacaggttt tgcatttgca agtttgacaa atgttaaaat gatggatatg gctgttgcag
    23761 ccttattccc aatattatta atagtgccat tatttgatat cttaatgtac tttaatattt
    23821 taccgaaaat tattggaggt attggttggt tactagctaa agtaacaaga caacctaaat
    23881 tcgagtcatt ctttgggata gaaatgatgt tcttaggaaa tactgaagca ttagccgtat
    23941 caagtgagca actaaaacgt atgaatgaaa tgcgtgtatt aacaatcgca atgatgtcaa
    24001 tgagctctgt atcgggagct attgtaggtg cgtatgtaca aatggtacca ggagaactgg
    24061 tactaacggc aattccacta aatatcgtta acgcgattat tgtgtcatgc ttgttgaatc
    24121 cagtaagtgt tgaagagaaa gaagatatta tttacagtct taaaaacaat gaagttgaac
    24181 gtcaaccatt cttctcattc cttggagatt ctgtattagc agcaggtaaa ttagtattaa
    24241 tcatcatcgc atttgttatt agttttgtag cgttagctga tctatttgat cgttttatca
    24301 atttgattac aggattgata gcaggatgga taggcataaa aggtagtttc ggtttaaacc
    24361 aaattttagg tgtgtttatg tatccatttg cgctattact cggtttacct tatgatgaag
    24421 cgtggttggt agcacaacaa atggctaaga aaattgttac aaatgaattt gttgttatgg
    24481 gtgaaatttc taaagatatt gcatcgtata caccacacca tcgtgcggtt attacaacat
    24541 tcttaatttc atttgcaaac ttctcaacga ttggtatgat tatcggtaca ttgaaaggca
    24601 ttgttgataa aaagacatca gactttgtat ctaaatatgt gcctatgatg ctattatcag
    24661 gtatcctagt ttcattatta acagcagctt tcgttggttt atttgcatgg taatatgttg
    24721 aagtgtgact tcgataatac attttaacta ataaatatgt tcaggcatgt cgtctattga
    24781 tataggtgag atgcttggac ttttttatta ttgatataaa gatatttaaa tatttttaaa
    24841 gttaccgaaa ttgaagcatt ataaaaacca agtgcacatg gtaatacact tggcttttat
    24901 gggaaatgaa tattattgta catatgacag taaggactag gtacagtcat agtacttcga
    24961 gcaaaatttg ttttgttatt ataaacaaca caaaggagat aacttctcta ttgaagaagt
    25021 taaaaacatt atagcagaca atgaaatgaa agtaaattaa aaattcagaa tatttttaat
    25081 tattatattg tgagtgatat ttataaggga aagcta
//
LOCUS       DAHHOJ010000030        25080 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.030, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000030 DAHHOJ010000000
VERSION     DAHHOJ010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 25080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25080
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.030"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(204..1151)
                     /locus_tag="NBH32_001038"
     CDS             complement(204..1151)
                     /locus_tag="NBH32_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HCD1514657.1"
                     /translation="MRLQKAPLVTSGLVLGLLGLGNLLKDLSLTLNAVCGIFAFLIWI
                     HLLCTMLKYSNNVKEQLNSPLVSSVFTTFFMSGFLGTTYLNTFFSNITFINSLITPIW
                     ILCLVGIMTHMIIFSIKYLKDFSLENVYPSWTVLFIGIAIAGLTAPVSGYFFIGQLTV
                     IYGFVATCIVLPIVFKRLKAFPLQTSIKPNTSTICAPFSLVAAAYVIAFPKANAFIVI
                     IFLILAQIFYFYIIIQLPKLLKEPFSPVFSAFTFPLVISATALKNSLPVLMFPDIWKG
                     LLFIEVLLATVIVLRVFIGYLHFFLKKENQDKFLRNASQ"
     gene            1400..1588
                     /locus_tag="NBH32_001039"
     CDS             1400..1588
                     /locus_tag="NBH32_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1514658.1"
                     /translation="MRNQIQKLLDSDLSSLHISKQTGVPQSTIHRMRKKERSLDNMSL
                     KNAELLYKFANSIFSNEN"
     gene            complement(2057..2130)
                     /locus_tag="NBH32_001040"
     tRNA            complement(2057..2130)
                     /locus_tag="NBH32_001040"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2094..2096),aa:Arg,seq:tct)
     gene            2494..2628
                     /locus_tag="NBH32_001041"
     CDS             2494..2628
                     /locus_tag="NBH32_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD1514659.1"
                     /translation="MEGLFNAIKDTVTAAINNDGAKLGTSIVSIVENGVGLLGKLFGF
                     "
     gene            2685..2819
                     /locus_tag="NBH32_001042"
     CDS             2685..2819
                     /locus_tag="NBH32_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-class phenol-soluble modulin"
                     /protein_id="HCD1514660.1"
                     /translation="MTGLAEAIANTVQAAQQHDSVKLGTSIVDIVANGVGLLGKLFGF
                     "
     gene            2943..3638
                     /locus_tag="NBH32_001043"
     CDS             2943..3638
                     /locus_tag="NBH32_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HCD1514661.1"
                     /translation="MGKLGYKNILIDFDDTIVDFYDAEEWAFHYMANVFNHKATKDDF
                     LTFKKINHQHWEAFQQNKLTKSEVLSERFVNYFKHHQMEVDGHRADVLFRNGLAEAKV
                     KYFDQTLETIVELSKRHDLYIVTNGVTETQKRRLNQTPLHKYIKKIFISEETGYQKPN
                     PEFFNYVFNDIGEDERQHSIIVGDSLTSDILGGINAGIATCWFNFRGFDHNPGIIPDY
                     EINSWKQLNDIVR"
     gene            complement(3744..4184)
                     /locus_tag="NBH32_001044"
     CDS             complement(3744..4184)
                     /locus_tag="NBH32_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1514662.1"
                     /translation="MSEIKRLEINYKTDELFENFRAFGNKDLYMVNELNGQMIDASSD
                     SPFYGIFVGDQLGARMALLKKGDVEEIYFPDFEDYILLWKLEVLPKYQNRGYASELID
                     FAKSFNMPIKAIGRNDSKDFFLHHGFTDVEAKNIEGHDVLLWKP"
     gene            4379..5992
                     /gene="bshC"
                     /locus_tag="NBH32_001045"
     CDS             4379..5992
                     /gene="bshC"
                     /locus_tag="NBH32_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000340457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis cysteine-adding enzyme
                     BshC"
                     /protein_id="HCD1514663.1"
                     /translation="MDCKVVSLNEKDQFIPKIKSSDPVITGLFQYDAAQQTSFEKRMS
                     KENNGREAALANVIREYMSDLKLSSEQELNIQHLANGSKVVIGGQQAGLFGGPLYTFH
                     KIFSIITLSKELTDTHKQQVVPVFWIAGEDHDFDEVNHTFVYNENHGSLHKVKYHTME
                     MPETTVSRYYPDKAELKQTLKTMFILMKETVHTQGLLEICDRIIDQYDSWTDMFKAIL
                     HETFKAYGVLFIDAQFEPLRKMEAPMFKKILKKHQLLDDAFRATQQRTQNQGLNAMIQ
                     TDTNVHLFLHDENMRQLVSYDGKHFKLNKTDKTYIKEEIINIAENQPELFSNNVVTRP
                     LMEEWLFNTVAFVGGPSEIKYWAELKDVFELFDVEMPIVMPRLRITYLNDRIEKLLSK
                     YNIPLEKVLVDGVEGERSKFIREQASHQFIEKVEGMIEQQRRLNQDLLDEVAGNQNNI
                     NLVNKNNEIHIQQYDYLLKRYLLNIERENEISMKQFREIQETLHPMGGLQERIWNPLQ
                     ILNDFGTDVFKPSTYPPLSYTFDHIIIKP"
     gene            6136..6567
                     /gene="mraZ"
                     /locus_tag="NBH32_001046"
     CDS             6136..6567
                     /gene="mraZ"
                     /locus_tag="NBH32_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCD1514664.1"
                     /translation="MFMGEYDHQLDTKGRMIIPSKFRYDLNERFIITRGLDKCLFGYT
                     LDEWQQIEEKMKTLPMTKKDARKFMRMFFSGAVEVELDKQGRINIPQNLRKYANLTKE
                     CTVIGVSNRIEIWDRETWNDFYEESEESFEDIAEDLIDFDF"
     gene            6583..7518
                     /gene="rsmH"
                     /locus_tag="NBH32_001047"
     CDS             6583..7518
                     /gene="rsmH"
                     /locus_tag="NBH32_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017724209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCD1514665.1"
                     /translation="MFHHISVMLNETIDYLNVKENGVYIDCTLGGAGHALYLLNQLND
                     DGRLIAIDQDQTAIDNAKEVLKDHLHKVTFVHSNFRELTQILKDLNIEKVDGIYYDLG
                     VSSPQLDIPERGFSYHHDATLDMRMDQTQELTAYEIVNNWSYEALVKIFYRYGEEKFS
                     KQIARRIEAHREQQPITTTLELVDIIKEGIPAKARRKGGHPAKRVFQALRIAVNDELS
                     AFEDSIEQAIELVKVDGRISVITFHSLEDRLCKQVFQEYEKGPEVPRGLPVIPEAYTP
                     KLKRVNRKPITATEEDLDDNNRARSAKLRVAEILK"
     gene            7532..7933
                     /gene="ftsL"
                     /locus_tag="NBH32_001048"
     CDS             7532..7933
                     /gene="ftsL"
                     /locus_tag="NBH32_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCD1514666.1"
                     /translation="MAVEKVYQPYDEQVYNSIPKQQPQTKPEKKTVSRKVVVQLTKFE
                     KVLYITLITVIAMLSIYMLSLKMDAYDTRGKIADLDYKIDKQSSENSALQSEIKKNSS
                     YERIYEKAKKQGMSLENDNVKVVRSNGEAKN"
     gene            7914..10148
                     /locus_tag="NBH32_001049"
     CDS             7914..10148
                     /locus_tag="NBH32_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1514667.1"
                     /translation="MAKQKIKIKKNKIGAVLLVGLFGLLFFILVLRISYIMITGHSNG
                     QDLVMKANEKYLVKNAQQPERGKIYDRNGKVLAEDVERYKLVAVIDKKASANSKKPRH
                     VVDKKETAKKLSTVIDMKPEEIEKRLSQKKAFQIEFGRKGTNLTYQDKLKIEKMNLPG
                     ISLLPETERFYPNGNFASHLIGRAQKNPDTGELKGALGVEKIFDSYLSGSKGSLRYIH
                     DIWGYIAPNTKKEKQPKRGDDVHLTIDSNIQVFVEEALDGMVERYQPKDLFAVVMDAK
                     TGEILAYSQRPTFNPETGKDFGKKWANDLYQNTYEPGSTFKSYGLAAAIQEGAFDPDK
                     KYKSGHRDIMGSRISDWNRVGWGEIPMSLGFTYSSNTLMMHLQDLVGADKMKSWYERF
                     GFGKSTKGMFDGEAPGQIGWSNELQQKTSSFGQSTTVTPVQMLQAQSAFFNDGNMLKP
                     WFVNSVENPVSKRQFYKGQKQIAGKPITKDTAEKVEKQLDLVVNSKKSHAANYRIDGY
                     EVEGKTGTAQVAAPNGGGYVKGPNPYFVSFMGDAPKKNPKVIVYAGMSLAQKNDQEAY
                     ELGVSKAFKPIMENTLKYLNVGKSKDDTSNAEYSKVPDVEGQDKQKAIDNVSAKSLEP
                     VTIGTGTQIKAQSIKAGNKVLPHSKVLLLTDGDLTMPDMTGWTKEDVIAFENLTNIKV
                     NLKGSGFVSHQSISKGQKLTEKDKIDVEFSSENVDSNSTNNSDSNSDDKKKSDSKTDK
                     DKSD"
     gene            10440..11405
                     /locus_tag="NBH32_001050"
     CDS             10440..11405
                     /locus_tag="NBH32_001050"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCD1514668.1"
                     /translation="MIFVYALLALVITFVLVPVLIPTLKRMKFGQSIREEGPQSHMKK
                     TGTPTMGGLTFLLSIVITSLVAIIFVDQANPIILLLFVTIGFGLIGFIDDYIIVVKKN
                     NQGLTSKQKFLAQIGIAIIFFVLSNVFHLVNFSTSIHIPFTNVAIPLSFAYVIFIVFW
                     QVGFSNAVNLTDGLDGLATGLSIIGFTMYAIMSFVLGETAIGIFCIIMLFALLGFLPY
                     NINPAKVFMGDTGSLALGGIFATISIMLNQELSLIFIGLVFVIETLSVMLQVASFKLT
                     GKRIFKMSPIHHHFELIGWSEWKVVTVFWAVGLISGLIGLWIGVH"
     gene            11407..12756
                     /locus_tag="NBH32_001051"
     CDS             11407..12756
                     /locus_tag="NBH32_001051"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCD1514669.1"
                     /translation="MLNYTGLENKNVLVVGLAKSGYEAAKLLSKLGANVTVNDGKDLS
                     QDAHAKDLESMGISVVSGSHPLTLLDNNPIIVKNPGIPYTVSIIDEAVKRGLKILTEV
                     ELSYLISEAPIIAVTGTNGKTTVTSLIGDMFKKSRLTGRLSGNIGYVASKVAQEVKPT
                     DYLVTELSSFQLLGIEKYKPHIAIITNIYSAHLDYHENLENYQNAKKQIYKNQTEEDY
                     LICNYHQRQVIESEELKAKTLYFSTQQEVDGIYIKDSFIVYKGVRIINTEDLVLPGEH
                     NLENILAAVLACILAGVPIKAIIDSLTTFSGIEHRLQYVGTNRTNKYYNDSKATNTLA
                     TQFALNSFNQPIIWLCGGLDRGNEFDELIPYMENVRAMVVFGQTKAKFAKLGNSQGKS
                     VIEANNVEDAVDKVQDIIEPNDVVLLSPACASWDQYSTFEERGEKFIERFRAHLPSY"
     gene            12772..14091
                     /locus_tag="NBH32_001052"
     CDS             12772..14091
                     /locus_tag="NBH32_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HCD1514670.1"
                     /translation="MDDKTKNDQQESNEDKDELELFTRNTSKKRRQRKRSKATHFSNQ
                     NKDDTSQQTDFDEEIYLINKDFKKEESNDENNDSASSHANNNNIDDSTDSNIENEDYR
                     YNQEIDDQNESNGIAVDNEQPQSAPKEQNSDSNDEETVTKKERKSKVTQLKPLTLEEK
                     RKLRRKRQKRIQYSVITILVLLIAVILIYMFSPLSKIAHVNINGNNHVSTSKINKVLG
                     VKNDSRMYTFSKKNAINDLEEDPLIKSVEIHKQLPNTLNVDITENEIIALVKYKGKYL
                     PLLENGKLLKGSNDVKINDAPVMDGFKGTKEDDMIKALSEMTPEVRRYIAEVTYAPSK
                     NKQSRIELFTTDGLQVIGDISTISKKMKYYPQMSQSLSRDSSGKLKTRGYIDLSVGAS
                     FIPYRGNTSSQSESDKNVTKSSQEENQAKEELQSVLNKINKQSSKNN"
     gene            14197..15603
                     /gene="ftsA"
                     /locus_tag="NBH32_001053"
     CDS             14197..15603
                     /gene="ftsA"
                     /locus_tag="NBH32_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCD1514671.1"
                     /translation="MEEHYYVSIDIGSSSVKTIVGEKFHNGINVIGTGQTYTSGIKNG
                     LIDDFDIARQAIKDTIKKASIASGVDIKEVFLKLPIIGTEVYDESNEIDFYEDTEING
                     SHIEKVLEGIREKNDVQETEVINVFPIRFIVDKENEVSDPKELIARHSLKVEAGVIAI
                     QKSILINMIKCVEACGVDVLDVYSDAYNYGSILTATEKELGACVIDIGEDVTQVAFYE
                     RGELVDADSIEMAGRDITDDIAQGLNTSYETAEKVKHQYGHAFYDSASDQDIFTVEQV
                     DSDETVQYTQKDLSDFIEARVEEIFFEVFDVLQDLGLTKVNGGFIVTGGSANLLGVKE
                     LLSDMVSEKVRIHTPSQMGIRKPEFSSAISTISSSIAFDELLDYVTINYHDNEETEED
                     VIDVKDKDNESKLGGFDWFKRKTNKKDTHENEVESTDEEIYQSEDNHQEHKQNHEHVQ
                     DKDKEESKFKKLMKSLFE"
     gene            15636..16808
                     /gene="ftsZ"
                     /locus_tag="NBH32_001054"
     CDS             15636..16808
                     /gene="ftsZ"
                     /locus_tag="NBH32_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCD1514672.1"
                     /translation="MLEFEQGFNHLATLKVIGVGGGGNNAVNRMIDHGMNNVEFIAIN
                     TDGQALNLSKAESKIQIGEKLTRGLGAGANPEIGKKAAEESREQIEDAIQGADMVFVT
                     SGMGGGTGTGAAPVVAKIAKEMGALTVGVVTRPFSFEGRKRQTQAAAGVEAMKAAVDT
                     LIVIPNDRLLDIVDKSTPMMEAFKEADNVLRQGVQGISDLIAVSGEVNLDFADVKTIM
                     SNQGSALMGIGVSSGENRAVEAAKKAISSPLLETSIVGAQGVLMNITGGESLSLFEAQ
                     EAADIVQDAADEDVNMIFGTVINPELQDEIVVTVIATGFDDKPTSHGRKSGSTGFGTS
                     VNTSSNATSKDESFTSNSSNAQATDSVSERTHTTKEDDIPSFIRNREERRSRRTRR"
     gene            17068..17859
                     /gene="pgeF"
                     /locus_tag="NBH32_001055"
     CDS             17068..17859
                     /gene="pgeF"
                     /locus_tag="NBH32_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HCD1514673.1"
                     /translation="MNDNFKKQPHHLIYEELLQQGITLGITTRGDGLSDYPKNAFNMA
                     RYIDDRPYNITQHQLQLAEEIAFDRKNWVFPIQTHENKVACITKDDIGTNIDTLTDAL
                     HGIDAMYTYDSNVLLTMCYADCVPVYFYSTKHHFIALAHAGWRGTYTEIVKEVLKHVN
                     FDLKDLHVVIGPSTSSSYEINDDIKNKFETLPIDSANYIETRGRDRHGIDLKKANAEL
                     LNYYGVPKENIYTTAYATSEHLELFFSYRLEKGQTGRMLAFIGQQ"
     gene            17883..18551
                     /locus_tag="NBH32_001056"
     CDS             17883..18551
                     /locus_tag="NBH32_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HCD1514674.1"
                     /translation="MKDNLQQISTQINDKSEKNNFSTKPNVIAVTKYVTIERAKEAYE
                     AGIRHFGENRLEGFFQKKEALPSDAVIHFIGSLQSRKVKDVINDVDYFHALDRLSLAK
                     EINKRAEHKIKCFLQVNVSGEASKHGIALEDVDQFIDDLKKYDKIEIVGLMTMAPLTD
                     DEAYIRSLFKQLRLKKEEIQRLNLEYAPCDELSMGMSNDYLIAVEEGATFVRIGTKLV
                     GEEE"
     gene            18548..19111
                     /locus_tag="NBH32_001057"
     CDS             18548..19111
                     /locus_tag="NBH32_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HCD1514675.1"
                     /translation="MSHLALKDLFSGFFVIDDEEEVEVPDKQQQVNEAPAKEQSQQTT
                     KQNAIKSVPQKSASRYTTTSEERNNRMSNYSKNNSRNVVTMNNATPNNASQESSKMCL
                     FEPRVFSDTQDIADELKNRRATLVNLQRIDKVSAKRIIDFLSGTVYAIGGDIQRVGTD
                     IFLCTPDNVEVAGSITDHIENMEHSFD"
     gene            19123..19413
                     /locus_tag="NBH32_001058"
     CDS             19123..19413
                     /locus_tag="NBH32_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HCD1514676.1"
                     /translation="MDINVLATIFKFILFVVEIYYFGMIIYFFTSWVPSIRETKVGYL
                     LAKIYEPFLQPFRKVIPPIGIIDISSIAAIIVLVLFQKGLLQIFNWILIQLQ"
     gene            19523..20302
                     /locus_tag="NBH32_001059"
     CDS             19523..20302
                     /locus_tag="NBH32_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001827493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HCD1514677.1"
                     /translation="MINIDIYQHFRQEEYELIDQLTDKCDQAEHHYAPVLTHFLDPRG
                     QYILEVICGSYEDLNVSFYGGPNAERKRAIISPNYYEPKESDFELTLMEIEYPEKFVT
                     LKHQHILGTLMSLGIEREQVGDIIVNERIQFVLTSRLESFIMLELQRIKGASVKLYTI
                     PVTDMIQSNENWKNESATVSSLRLDVVIKEMIRKSRTIAKQLIEKKRVKVNHTIVDSA
                     DFQLQANDLISIQGFGRAHITDLGGKTKKDKTHITYRTLFK"
     gene            20326..20943
                     /locus_tag="NBH32_001060"
     CDS             20326..20943
                     /locus_tag="NBH32_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HCD1514678.1"
                     /translation="MPFTPNEIKNKEFSRVKNGLEPTEVANFLEQLSTEIERLKEDKK
                     QLEKVIEERDTNIKSYQDVHQSVSDALIQAQKAGEETKQAAEKQAEAIIAKAEAQANQ
                     MVGDAVEKARRLAFQTEDMKRQSKVFRSRFRMLVEAQLDLLKNEDWDYLLNYDLDAEQ
                     VTLENIHHLHENDLKPDEVAANAQNNASNTPDNNQQSNDSETTKK"
     gene            21164..23917
                     /gene="ileS"
                     /locus_tag="NBH32_001061"
     CDS             21164..23917
                     /gene="ileS"
                     /locus_tag="NBH32_001061"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCD1514679.1"
                     /translation="MDYKETLLMPKTDFPMRGGLPNKEPQIQEKWDAEDQYHKALEKN
                     KGNETFILHDGPPYANGNLHMGHALNKILKDFIVRYKTMQGFYAPYVPGWDTHGLPIE
                     QALTKKGVDRKKMSTAEFREKCKEFALEQIELQKKDFRRLGVRGNFNNPYITLKPEYE
                     AAQIRIFGEMADKGLIYKGKKPVYWSPSSESSLAEAEIEYHDKRSASIYVAFDVKDDK
                     GVVDADAKFIIWTTTPWTIPSNVAITVHPELKYGQYNVNGEKYIIAEALSDAVAEALD
                     WDKASIKLEKEYTGKELEYVVAQHPFLDRESLVINGDHVTTDAGTGCVHTAPGHGEDD
                     YIVGQKYELPVISPIDDKGVFTEEGGQFEGMFYDKANKAVTDLLTEKGALLKLDFITH
                     SYPHDWRTKKPVIFRATPQWFASISKVRQDILDAIENTNFKVNWGKTRIYNMVRDRGE
                     WVISRQRVWGVPLPVFYAENGEIIMTKETVNHVADLFAEHGSNIWFEREAKDLLPEGF
                     THPGSPNGTFTKETDIMDVWFDSGSSHRGVLETRPELSFPADMYLEGSDQYRGWFNSS
                     ITTSVATRGVSPYKFLLSHGFVMDGEGKKMSKSLGNVIVPDQVVKQKGADIARLWVSS
                     TDYLADVRISDEILKQTSDVYRKIRNTLRFMLGNINDFNPDTDSIPESELLEVDRYLL
                     NRLREFTASTINNYENFDYLNIYQEVQNFINVELSNFYLDYGKDILYIEQRDSHIRRS
                     MQTVLYQILVDMTKLLAPILVHTAEEVWSHTPHVKEESVHLADMPKVVEVDQALLDKW
                     RTFMNLRDDVNRALETARNEKVIGKSLEAKVTIASNDKFNASEFLTSFDALHQLFIVS
                     QVKVVDKLDDQATAYEHGDIVIEHADGEKCERCWNYSEDLGAVDELTHLCPRCQQVVK
                     SLV"
     gene            24208..25005
                     /locus_tag="NBH32_001062"
     CDS             24208..25005
                     /locus_tag="NBH32_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1514680.1"
                     /translation="MYHNSNANFVNGITLNVRDKNELKPFYEDVLGLNIINETLTSIQ
                     YEVGQNNHVITLVELQNGREPLMSEAGLFHIAIKLPQISDLANLLIHLSEYDIPVNGG
                     IQPASLSLFFEDPEGNGFKFYVDKDEAQWTRQNDLVKIDIRPLNVPRLVSHATKLLWL
                     GIPDDAIIGALHIKTIHLSEVKEYYLDYFGLEQSAYMDDYSIFLASNGYYQHLAMNDW
                     VSATKRVENFDTYGLAIVDFHYLETTHLNLQGPDGIYYRFNHIEVEG"
BASE COUNT         9271 a         3447 c         4736 g         7626 t
ORIGIN      
        1 gcttatatat tatttaaata aaggttagag tgaaacgtgt ttatgaacta gacgttctag
       61 tatagttact acagctttag tcagaaggta tcattgatat gatcatatta aatcaaagag
      121 gcattgatat acactaaaaa gaggcaagat tacctgcctc ttttttagtt attaaatatg
      181 cgtgttaatt cttggtaata gtgttactga gacgcattac gaagaaattt atcttgattt
      241 tcctttttta aaaagaagtg aagatatcct ataaagactc taagtactat tacagtagct
      301 aataacactt cgataaacaa aagacctttc caaatgtctg gaaacataag tacaggcaaa
      361 ctgttcttta aagcagttgc tgagattact aaagggaatg tgaaagctga aaatacgggc
      421 gaaaaaggtt cttttagtaa tttaggcaat tgtataatga tataaaaata aaatatttga
      481 gctaatatta aaaatataat tacgataaaa gcattcgcct taggaaatgc tataacatat
      541 gctgcagcga ctaaagaaaa tggtgcacaa attgtcgatg tgttcggttt gattgacgtc
      601 tgcaatggaa atgcttttaa tcgcttgaaa acaataggta agacaataca agtagctaca
      661 aagccatata ttactgttaa ttgacctatg aaaaaatatc cgctaacggg tgccgtcaat
      721 cctgcgatag caataccaat aaaaagtaca gtccacgaag gataaacatt ttcaagtgaa
      781 aaatctttta aatattttat tgaaaaaata atcatatgcg tcataattcc cacaaggcat
      841 aaaatccaaa taggcgttat taagctattg ataaaagtta tgttactaaa aaatgtattt
      901 aaataagtag tacctaaaaa gccagacatg aaaaatgttg tgaacactga tgaaactaga
      961 ggactgttta attgttcttt cacattatta gaatatttga gcatagtaca taaaaggtga
     1021 atccaaatca agaaagcaaa gattccgcaa acagcgttta aagtaagaga taagtctttt
     1081 aatagattac ccaggcctaa taatcctaag actagtcctg acgttactaa aggtgctttt
     1141 tgaagtctca tgatttacaa cctttcttat gtgattttct tcactaatta tatcatgata
     1201 gctatggcca attaataaga agagtgtgta ctattacgtt attagagtat gtatattgga
     1261 ttgatagaca cataattgac atttaaatct caaaattaat gatatttgag gtatgctttg
     1321 caacctaaat attggaatat gtggaaaagt aattatttaa tttaaatgat gattgaagat
     1381 ttacaagggg gtgtacaaaa tgagaaatca aattcaaaaa ctattagaca gtgatttgag
     1441 cagtttacat atatcgaaac aaacaggagt tccacaaagc acaatacaca gaatgagaaa
     1501 aaaagaaaga tcattagaca atatgtcatt gaaaaacgct gaactacttt ataaatttgc
     1561 caatagtata tttagcaatg aaaattaaga agaataatta gtgaattaaa taattaaaaa
     1621 accacgtctt gatggacgtg gttatttttt taagatgtat ataattatag gatttcgggt
     1681 ccctgaaaag tccctaaaat tttgaatagt atcaaaatct ataagaataa tataataata
     1741 aaaatctcta cgcatgcaaa tggtgagtgt taaaaatctt ggtgtagtgt tggtgtagtt
     1801 caggtgcagt atagatgaaa tttgagcaca aaattactta attaaaaatg tataaacaag
     1861 tgtcagacgc gtgtcaattg tgtgtcaaaa aattctataa cgaataagca tgcttgtttt
     1921 aattactgta ttttcggggc ttatgcgggg gacgttttgg tgacgcatca tactataata
     1981 ctgaaattca aaacaaaaag agccccgtaa tcacggaact cttttgtttg gtaatgcgta
     2041 taaaaatacc tataaacgtc ctgggaggga ttcgaacccc cgaccgatgg cttagaaggc
     2101 cattgctcta tccagctgag ctaccaggac acgtttaaca acacaagaat tattatatct
     2161 aaatgaactt aaattagcaa taccttgtaa ataaaaaatg tttatatttt tcactattat
     2221 agagctattt atctaaaaag gttcaataag acttaaatac gaattcaggc aacttaattg
     2281 tgttaaatac agttttgaat gcctaactgt atttcttttc tctttaaaat acagttaagt
     2341 acattataag atgttgtgcg gataaacaaa ctaattgcat caaatttatt ttaaaataac
     2401 aacaacaaaa cgttaagcga ataacatttc ggtgatttaa aagctacgca cgtttttgtt
     2461 atcttcaaat ttaaatttta aggagtgttt tcaatggaag gtttatttaa cgcaattaaa
     2521 gataccgtaa ctgcagcaat taataatgac ggcgcaaaat taggcacaag cattgtgagc
     2581 atcgttgaaa atggcgtagg tttattaggt aaattattcg gattctaata tcaatatgtt
     2641 atgtaagtaa taagtattat ttcaaaggtg agggagagat ttaaatgact ggactagcag
     2701 aagcaatcgc aaatactgtg caagctgcac aacaacatga tagtgtgaaa ttaggcacaa
     2761 gtatcgtaga catcgttgct aacggtgtgg gtttactagg taaattattt ggattctaat
     2821 ataataacta atattcttta aaataaactg ggtgagcata ctttaatgtt atgcactcag
     2881 tttattttat ttgcagaaat ttgagcctct gttaagattt agatacatag acaatatagg
     2941 agatggggaa attgggatat aaaaatattt tgatagactt tgatgataca attgttgatt
     3001 tttatgatgc agaagaatgg gcgtttcact atatggcgaa tgtttttaat cataaagcaa
     3061 caaaagatga ttttttaaca tttaaaaaaa tcaatcacca acattgggaa gcttttcaac
     3121 aaaataaatt aacgaagtct gaagtattat cagaacgatt tgtgaattac ttcaaacatc
     3181 atcaaatgga agttgatggg catcgtgcag atgtgttatt tagaaatgga ttagcagaag
     3241 ctaaagttaa atactttgat caaacattag aaacaattgt cgaattatcg aaaagacatg
     3301 atttatatat tgttactaat ggtgtaaccg aaacgcaaaa gagaaggtta aatcagacgc
     3361 cgttgcataa atatattaaa aagatattta tatctgagga aacaggatat caaaaaccta
     3421 atccggaatt ttttaattat gtttttaatg atattggtga ggatgaaaga cagcactcga
     3481 ttatagttgg agattcttta acatctgaca ttctaggtgg aatcaatgcg ggtatagcta
     3541 cttgctggtt taattttaga ggatttgatc ataatccagg aattatacct gattatgaaa
     3601 ttaattcatg gaaacaacta aatgatattg tacgttaaga caaataattt gaaaatgatt
     3661 tactattagc ttaagaaagt aattgtaaaa aagacactac gaaaagatgc attttcatgc
     3721 tcgtagtgtc gaatattatt gtcttatggt ttccacaata agacatcatg tccctctata
     3781 tttttagctt ccacatctgt aaatccatga tgtaaaaaga aatccttaga atcatttctg
     3841 ccaatggctt taattggcat gttgaaactc tttgcaaaat caatcaattc tgaagcgtac
     3901 cctctgtttt gatattttgg taatacttct aacttccata ataatatata atcttcaaaa
     3961 tctgggaagt agatttcttc gacatcacct ttttttagta atgccattct agctcctaat
     4021 tgatctccga caaatatgcc ataaaatggt gaatctgaac ttgcatcaat catttgaccg
     4081 tttaactcat tgaccatgta taagtctttg ttgccaaacg ctctaaagtt ttcgaataat
     4141 tcgtcagttt tgtaattaat ttcaagacgt ttgatttcac tcatatttat ctacccctta
     4201 tttgtttact actattatta tacattaaac cacttgtgtt tttcattgat ttgaaaatgt
     4261 aaaacaatga atattataaa ttttccatga ttaaattgat tttcaagccg taaaattgtc
     4321 aatcaccggt atagacttta tactatagtt tgtaatcgcg tacttaagga gaaaaaacat
     4381 ggactgtaaa gtagttagtt taaatgaaaa agatcagttt ataccaaaaa taaagagcag
     4441 tgaccctgta ataacaggat tatttcaata tgacgcagct caacaaacta gttttgaaaa
     4501 aaggatgtct aaagaaaata atggaagaga agcggcatta gcgaatgtta ttcgtgaata
     4561 tatgagtgat ttaaagcttt ctagtgaaca agaattaaac atacaacatt tagctaatgg
     4621 ttcaaaagtt gtgattggtg gacaacaagc agggcttttc gggggaccat tgtatacatt
     4681 ccataaaata ttttcaatca ttactttatc taaggaatta acggatacac ataagcaaca
     4741 agtagtacca gttttttgga ttgcaggaga agatcatgat ttcgatgaag tgaatcatac
     4801 atttgtttat aacgaaaatc atgggtcgct gcataaggtt aaatatcata caatggagat
     4861 gccagagacg actgtctcta gatattatcc tgataaggct gagttgaaac aaactttaaa
     4921 aacgatgttc attcttatga aagaaactgt tcatacacaa ggtctactgg agatttgtga
     4981 cagaattatt gaccaatatg actcgtggac tgatatgttt aaagcaatac tgcatgaaac
     5041 atttaaagca tatggcgttc tatttataga tgcgcagttt gagccgttaa gaaaaatgga
     5101 agcgcctatg tttaaaaaga ttttgaaaaa acatcagttg cttgatgatg cttttagagc
     5161 aacacaacaa cgtactcaaa atcaaggctt gaatgcgatg atacaaacag atacaaatgt
     5221 tcatttattc ttacatgatg aaaatatgcg tcaattagtt tcgtatgatg gtaagcattt
     5281 taaattaaat aaaacagata agacatatat aaaggaagaa attataaata ttgcggaaaa
     5341 tcaacctgaa ttattttcta ataatgtagt gacaagacca ttaatggaag aatggttatt
     5401 taacacggtg gcatttgttg gaggaccgag tgaaattaag tactgggctg aactaaaaga
     5461 tgtatttgaa ctatttgatg ttgaaatgcc tatcgtgatg ccaaggctta gaattactta
     5521 tttaaatgac cgtatagaaa aattactttc gaaatacaat attccattag aaaaagtgtt
     5581 agtcgatggt gttgaaggag aaagaagtaa gtttattaga gaacaagcat cacatcaatt
     5641 tattgaaaag gtagaaggta tgattgaaca acagcgtcgt ctaaaccaag acttattaga
     5701 tgaagtggcg gggaatcaaa ataatattaa ccttgtgaat aaaaataatg aaattcatat
     5761 acaacagtat gattatttgt taaaacgtta tcttttaaac attgaaagag aaaacgagat
     5821 cagtatgaag caatttagag aaattcaaga aacactccat ccaatgggag gattacaaga
     5881 aagaatatgg aatccacttc aaattttgaa tgattttggg acagatgtgt tcaagccctc
     5941 cacctatcca ccactttctt acacttttga tcatattatt ataaaacctt aatataccaa
     6001 gggtttagcc cgatttatct taatgataaa tcgggcattt ttttgttttt taaaataaat
     6061 ttcacaaatt ttgtataaat agtggtggat agtggggaga tgtggtaaat tatatataag
     6121 gtgaggtgat aaaaaatgtt catgggagaa tacgatcatc aattagatac aaaaggacgt
     6181 atgattatac cgtccaagtt tcgttatgac ttaaatgagc gttttattat cacaagaggc
     6241 cttgataaat gtttattcgg ttacactcta gacgaatggc aacagattga agagaaaatg
     6301 aaaaccttac ctatgacaaa aaaagacgca cgtaagttta tgcgtatgtt cttctctggt
     6361 gctgttgaag tagaacttga taagcaaggg cgtattaaca tccctcaaaa cttgaggaaa
     6421 tacgctaatt taactaaaga atgtacagta atcggtgttt caaatcgtat tgagatttgg
     6481 gatagagaaa cttggaatga tttctatgaa gaatctgaag aaagtttcga agatattgct
     6541 gaagatttaa tagattttga tttttaaaat ggaggaattg aagtgtttca tcatatcagc
     6601 gttatgttaa acgaaaccat tgattattta aatgtaaaag aaaatggtgt gtacattgac
     6661 tgtacgctag gtggagcggg acatgccctt tatttactaa atcaattaaa tgacgacgga
     6721 agattaatag caatcgatca agaccaaact gcaattgata atgctaaaga ggtattgaag
     6781 gatcatttgc ataaggtgac ttttgttcat agcaacttcc gtgaattaac tcaaatatta
     6841 aaagacttaa acattgaaaa agtagatgga atttattacg acttgggtgt ttcaagccca
     6901 caactcgaca ttccagaacg aggattcagt tatcaccatg acgcaacatt agacatgcgt
     6961 atggaccaaa cacaagaact aacagcatat gaaattgtta acaattggtc atatgaagcg
     7021 ttagtgaaga ttttttatcg ttatggcgag gagaaatttt caaaacagat agctcgaaga
     7081 atcgaagcac atcgtgaaca acaaccaata acaacaacat tagaattagt tgacattata
     7141 aaagaaggta ttcctgcaaa agcaagaaga aaaggcggac atcctgcaaa acgagtattt
     7201 caagcactac gaattgcagt aaacgatgaa ttgtcagctt ttgaagattc aatagaacaa
     7261 gcgattgaat tagtgaaagt agacggcagg atatcggtaa tcactttcca ttctttagaa
     7321 gatcgtttat gtaaacaggt gttccaagaa tatgaaaaag gtccagaggt accaagagga
     7381 ttaccagtta taccagaagc atatacacct aagttaaagc gtgttaatcg taaaccgatt
     7441 accgctacag aagaagattt agatgacaat aacagagcac gaagcgcgaa attacgtgta
     7501 gctgaaatac ttaaataagg agcaatttat aatggctgta gaaaaagtgt accaaccata
     7561 tgacgaacaa gtttataata gtataccgaa gcaacaacca caaactaagc ccgaaaagaa
     7621 gactgtttcg agaaaagtgg ttgtacaatt aactaaattt gaaaaagttt tatacataac
     7681 tttgattact gtaattgcta tgttaagtat ttatatgcta tctttaaaaa tggatgcgta
     7741 tgatacgcga ggaaagattg cagatttaga ttataaaata gataaacaat caagtgaaaa
     7801 cagtgcttta caatctgaaa tcaaaaagaa ttcttcttat gaacgcatat acgaaaaggc
     7861 taagaaacag gggatgagcc ttgagaacga taatgtaaag gtagtgcgta gtaatggcga
     7921 agcaaaaaat taaaattaaa aaaaataaaa taggggcagt cctacttgtt ggtttattcg
     7981 gactgctctt ttttatattg gttttaagaa tttcatatat catgattact ggacattcta
     8041 atggtcaaga tttagtcatg aaggcaaatg aaaagtattt agttaagaat gcacaacaac
     8101 cagaacgagg gaagatatat gatcgtaatg gtaaagtgct agcagaagat gtagaaagat
     8161 ataaacttgt tgcagtaata gataaaaagg cgagtgccaa ttctaaaaaa cctaggcatg
     8221 tagttgataa aaaagagact gcaaagaaat tatctacagt cattgatatg aagccagagg
     8281 aaattgaaaa gagacttagt caaaagaaag ctttccaaat tgaatttgga cgcaaaggaa
     8341 caaatttaac gtatcaggac aaattgaaaa tagagaaaat gaatttgcct ggtatttctt
     8401 tattgcctga aacagaacgc ttttatccaa atggcaattt tgcatcacac ttaattggta
     8461 gagctcagaa aaatccagat actggtgaac ttaaaggtgc gcttggagtt gaaaagattt
     8521 ttgatagtta tttaagtgga tctaaaggat cattgagata tattcatgat atttggggat
     8581 atatcgcacc aaatactaaa aaagagaagc agcctaaacg tggtgatgat gtccatttaa
     8641 caatcgattc aaatattcaa gtatttgttg aagaagcttt agatggcatg gttgaaagat
     8701 accagccgaa agatttattt gcggttgtca tggatgccaa aactggagaa attttagcat
     8761 acagtcagcg accaacattt aatcctgaaa ctggtaaaga ctttggtaaa aagtgggcaa
     8821 atgacctata tcaaaataca tacgagcctg gatcaacatt taaatcatat gggttagcag
     8881 ctgctattca agaaggtgct tttgatcctg ataagaaata taaatctgga catagagata
     8941 ttatgggttc acgtatttca gactggaata gagtcggttg gggtgaaatc ccaatgtcac
     9001 tcggatttac ttattcatct aatacattga tgatgcattt acaagattta gttggtgcag
     9061 acaaaatgaa atcttggtat gaacgatttg gatttggaaa atcaactaaa ggtatgtttg
     9121 atggagaagc acctggtcaa attggatgga gtaatgagtt gcaacaaaaa acgtcatcat
     9181 ttggtcaatc gacaactgta acacctgttc aaatgttaca agcgcaatca gcgttcttta
     9241 atgatggtaa tatgttaaaa ccatggtttg tgaatagcgt tgaaaatcct gttagtaaaa
     9301 gacaatttta taaaggtcaa aaacaaatcg caggcaaacc aataacaaaa gatactgctg
     9361 aaaaagttga aaagcaattg gatttagttg tgaatagtaa gaagagtcac gctgcaaact
     9421 atcgtattga tggttatgag gtcgaaggta agactggtac agcacaagtc gctgcaccta
     9481 atggtggtgg atacgttaaa ggtccaaacc catattttgt aagttttatg ggtgacgcgc
     9541 cgaagaaaaa tcctaaagtt attgtatacg ctggtatgag cttggcacaa aaaaatgacc
     9601 aagaagctta tgaattaggt gttagtaaag cgtttaaacc aataatggaa aatactttga
     9661 aatatttaaa tgtaggtaaa tcaaaagatg acacatctaa tgcagagtat agtaaagtgc
     9721 cagatgttga aggtcaagac aaacaaaaag ctattgataa tgtgagtgca aaatcattag
     9781 aaccagttac tatcggtact ggcacacaaa taaaagcaca atctataaaa gcagggaata
     9841 aagtcttacc tcatagtaaa gtactgttat taacagatgg agacttaact atgcctgaca
     9901 tgacaggatg gacgaaagaa gatgtcattg cttttgaaaa cctaacaaat attaaagtaa
     9961 atttaaaagg tagcggtttt gtgtcccacc aatcaattag taagggacaa aaacttactg
    10021 aaaaagataa aatagacgta gaattttcat cagagaatgt agacagcaat tcgacgaata
    10081 attctgattc gaattcagat gataagaaga aatctgacag taaaactgac aaggataagt
    10141 cggactaaca agaatggcta cattaaataa gggtgtattg tcactatttg tttttactaa
    10201 agtaatgctg tgatagaaaa caacatcttc atttaatctt tataaataag tgttgataga
    10261 gaattttaag gtagacgagg tcagattaac ttgtttgtta taggcaaact ttttatactg
    10321 gcctcttatg ccgtaaaaaa gaaaaattat aaattttgat tacaactttg tttatttaat
    10381 tgaaaaattg ataatatatt agtggttaaa aaagtctgga ttaagaagag gagatagtta
    10441 tgatttttgt atatgcgtta ttagcgctag tgattacatt tgttttggta cctgttttaa
    10501 tacctacatt aaaaaggatg aaatttggtc aaagtattcg agaagaaggt ccacaaagcc
    10561 atatgaagaa gactggtaca ccaacgatgg gtggactaac atttctatta agtattgtga
    10621 taacgtcttt ggtggctatt atatttgtag atcaagctaa tccaatcata ctgttattat
    10681 ttgtgacgat tggttttggg ttaattggtt ttatagatga ttatattatt gttgttaaaa
    10741 agaataacca aggtttaaca agtaaacaga agtttttggc gcaaattggt attgcgatta
    10801 ttttctttgt tttaagtaat gtgtttcatt tggtgaattt ttctacgagc atacatattc
    10861 catttacgaa tgtagcaatc ccactatcat ttgcatatgt tattttcatt gttttttggc
    10921 aagtaggttt ttctaatgcg gtaaatttaa cagatggttt agatggatta gcaactggac
    10981 tgtcaattat cggatttaca atgtatgcca tcatgagctt tgtgttagga gaaacggcaa
    11041 ttggtatttt ctgtatcatt atgttgtttg cacttttagg atttttacca tataacatta
    11101 accctgctaa agtgtttatg ggagatacag gtagcttagc tttaggtggt atatttgcta
    11161 cgatttcaat catgcttaat caggaattat cattaatttt tataggttta gtattcgtaa
    11221 ttgaaacatt atccgttatg ttacaagtcg ctagctttaa attgactgga aagcgtatat
    11281 ttaaaatgag tccgattcat catcattttg aattgatagg atggagcgaa tggaaagtag
    11341 ttacagtatt ttgggccgtt ggtctgattt caggtttaat cggtttatgg attggagtgc
    11401 attaagatgc ttaattatac agggttagaa aataaaaatg tattagttgt cggtttggca
    11461 aaaagtggtt atgaagcagc taaattatta agtaaattag gtgcgaatgt aactgtcaat
    11521 gatggaaaag acttatcaca agatgctcat gcaaaagatt tagaatctat gggcatttct
    11581 gttgtaagtg gaagtcatcc attaacgttg cttgataata atccaataat tgttaaaaat
    11641 cctggaatac cttatacagt atctattatt gatgaagcag tgaaacgagg tttgaaaatt
    11701 ttaacagaag ttgagttaag ttatctaatc tctgaagcac caatcatagc tgtaacgggt
    11761 acaaatggta aaacgacagt tacttctcta attggagata tgtttaaaaa aagtcgctta
    11821 actggaagat tatccggcaa tattggttat gttgcatcta aagtagcaca agaagtaaag
    11881 cctacagatt atttagttac agagttgtcg tcattccagt tacttggaat cgaaaagtat
    11941 aaaccacaca ttgctataat tactaacatt tattcggcgc atcttgatta ccatgaaaat
    12001 ttagaaaact atcaaaatgc taaaaagcaa atatataaaa atcaaacgga agaagattat
    12061 ttgatttgta attatcatca aagacaagtg atagagtcgg aagaattaaa agctaagaca
    12121 ttgtatttct caactcaaca agaagttgat ggtatttata ttaaagatag ttttatcgtt
    12181 tataaaggtg ttcgtattat taacactgaa gatctagtat tgcctggtga acataattta
    12241 gaaaatatat tagcagctgt gcttgcttgt attttagctg gtgtacctat taaagcaatt
    12301 attgatagtt taactacatt ttcaggaata gagcatagat tgcaatatgt tggtactaat
    12361 agaactaata aatattataa tgattccaaa gcaacaaaca cgctagcaac acagtttgcc
    12421 ttaaattcat ttaatcaacc aatcatttgg ttatgtggtg gtttggatcg agggaatgaa
    12481 tttgacgaac tcattcctta tatggaaaat gttcgcgcga tggttgtatt cggacaaacg
    12541 aaagctaagt ttgctaaact aggtaatagt caagggaaat cggtcattga agcgaacaat
    12601 gtcgaagacg ctgttgataa agtacaagat attatagaac caaatgatgt tgtattattg
    12661 tcacctgctt gtgcgagttg ggatcaatat agtacttttg aagagcgtgg agagaaattt
    12721 attgaaagat tccgtgccca tttaccatct tattaaaggg tgtgagtatt gatggatgat
    12781 aaaacgaaga acgatcaaca agaatcaaat gaagataaag atgaattaga attatttacg
    12841 aggaatacat ctaagaaaag acggcaaaga aaaagatcaa aggctacaca tttttctaat
    12901 caaaataaag atgatacatc tcaacaaact gattttgatg aagaaattta cttgataaat
    12961 aaagacttca aaaaagaaga aagcaatgat gaaaataatg attctgcttc tagtcatgcg
    13021 aataataata atatcgatga ttctacagac tctaatattg aaaatgagga ttatagatat
    13081 aatcaagaaa ttgacgacca aaatgaatcg aatggaattg cagtcgacaa cgaacaacct
    13141 caatcagctc ctaaagaaca aaatagcgac tcgaatgatg aggaaacagt aacgaaaaaa
    13201 gaacgaaaaa gtaaagtaac acaattaaag ccattaacac ttgaagaaaa gcggaagtta
    13261 agacgtaagc gacaaaaacg aatccaatac agtgttatta caatattggt attgttgatt
    13321 gctgttatat taatttacat gttttcacca cttagtaaaa ttgcgcatgt aaatataaat
    13381 ggaaataatc acgttagtac ttcaaagata aacaaagttt taggtgttaa aaatgattca
    13441 aggatgtata cgtttagtaa aaaaaatgct attaatgatc tcgaagagga tccattaatc
    13501 aaaagtgttg agatacacaa gcaattacca aacacattaa acgtagatat cacagaaaat
    13561 gaaattattg ctttagtgaa atataaaggt aaatatttac ctttattaga aaatggtaaa
    13621 ttgcttaaag gttcaaatga tgtcaaaatt aatgatgcac ctgtcatgga tggtttcaaa
    13681 ggtacaaaag aagatgatat gattaaggcg ttatctgaaa tgacacctga agttagacga
    13741 tatattgccg aagtgacata cgccccaagt aaaaacaaac aaagcagaat tgaattgttt
    13801 acgacagatg gacttcaagt aatcggtgat atttcgacga tatctaagaa aatgaaatat
    13861 tatccgcaga tgtcacaatc attatcaagg gatagttcgg gtaaactaaa aacacgaggc
    13921 tatattgatt tatcagtcgg tgcttcattt atcccatacc gtggaaacac gtctagtcaa
    13981 tcagaaagcg ataaaaatgt gactaaatca tctcaagagg aaaatcaagc aaaagaagaa
    14041 ttacaaagcg ttttaaacaa aattaacaaa caatcaagta agaataatta aaaaattttg
    14101 atattgtcta tgtttatagt tcacaagcca ttcaacgtat tgtaaactaa ggatagtgta
    14161 tttttttaat agtaatttgt caggaggtgc ctatctatgg aagaacatta ctacgtaagt
    14221 attgatattg gatcatcaag cgtaaaaaca atagtaggcg agaaatttca caatggtata
    14281 aatgtgatag gtacaggaca aacctacacg agcggtataa aaaatggttt aattgatgat
    14341 tttgatattg cgcgacaagc aatcaaagac acaattaaaa aggcatcaat cgcttcgggt
    14401 gttgatatta aagaagtttt cctgaaatta cctatcattg gaacggaagt ttatgatgaa
    14461 tcaaatgaaa tcgactttta tgaggataca gaaatcaacg gttcacatat cgaaaaagta
    14521 ttagaaggta ttagagaaaa aaatgatgtg caagaaacag aagtaattaa tgtgttcccg
    14581 attcgtttta tagtcgataa agaaaatgag gtttcagacc ctaaagaatt aattgccaga
    14641 cattcattaa aggttgaagc aggcgtaatt gctattcaaa aatcgatttt aattaatatg
    14701 attaaatgcg ttgaagcatg tggtgttgat gtattagatg tttactctga tgcatataac
    14761 tatggttcaa tcctaacagc tactgaaaaa gagttaggtg catgtgtcat tgatattggt
    14821 gaagacgtta cgcaagttgc tttttatgaa cgcggtgaat tagtagatgc tgattctatc
    14881 gaaatggcag ggcgtgatat tacagacgat attgcacaag gattaaacac ttcttatgaa
    14941 actgctgaaa aagttaaaca ccaatatggt catgcattct atgattctgc ttcagatcaa
    15001 gatatcttca ctgttgaaca ggttgatagt gatgaaacag tacagtatac tcaaaaagat
    15061 ttgagtgact ttattgaagc gcgtgtagaa gaaatattct tcgaagtatt tgatgtttta
    15121 caagatttag gattaacaaa agtaaatggt gggtttattg taactggtgg atctgcaaac
    15181 ttacttggcg taaaagaatt attatcagat atggtaagtg aaaaagttag aattcacacg
    15241 ccatcgcaaa tgggaattag aaaacctgaa ttttcttcag caatttctac aatttctagt
    15301 agtatcgctt ttgatgagtt attagattat gttacaatta attatcatga taatgaagaa
    15361 actgaagaag atgttattga tgtgaaagac aaagataacg aatctaaatt aggcggattt
    15421 gattggttta aacgtaaaac aaacaaaaaa gatactcatg aaaatgaagt agagtcaaca
    15481 gatgaagaaa tttatcaatc agaagataat catcaggaac ataaacagaa tcatgaacat
    15541 gttcaagaca aagataaaga agaaagtaaa ttcaaaaaac taatgaaatc tctatttgaa
    15601 tgattattgg ccaataaaac taggaggaaa tttaaatgtt agaatttgaa caaggattta
    15661 atcatttagc gactttaaag gtcattggtg taggtggtgg cggtaacaac gccgtaaacc
    15721 gaatgattga ccatggaatg aataatgttg aatttatcgc tatcaacaca gacggtcaag
    15781 ctttaaactt atctaaagct gaatctaaaa tccaaatcgg tgaaaaatta acacgtggtt
    15841 taggagcagg agctaatcct gaaatcggta aaaaagctgc agaggaatct cgtgaacaaa
    15901 ttgaagatgc aatccaaggt gcagacatgg tatttgttac ttctggtatg ggtggcggaa
    15961 ctggtactgg tgcagcacca gtcgttgcta aaattgcaaa agaaatgggc gcattaactg
    16021 ttggtgttgt aactcgtcca tttagttttg aaggacgtaa acgtcaaact caagctgctg
    16081 ctggagtaga agctatgaaa gctgcagtag atacattaat cgttatacca aatgaccgtt
    16141 tattagatat cgttgacaaa tctacgccaa tgatggaagc atttaaagaa gctgataacg
    16201 tgttacgcca aggtgtacaa ggtatctcag acttaatcgc tgtttctggt gaagtaaact
    16261 tagactttgc agacgttaag acaattatgt ctaaccaagg ttctgcatta atgggtattg
    16321 gtgtttcttc tggtgaaaat agagcggtag aagctgctaa aaaagcaatc tcttctccat
    16381 tacttgaaac atctatcgtt ggtgcacaag gtgtgcttat gaatattact ggtggcgagt
    16441 cattgtcatt atttgaagca caagaggctg ctgatattgt ccaagatgct gcagatgaag
    16501 acgttaatat gattttcggt acagttatta atcctgaatt acaagatgag attgttgtaa
    16561 cagttattgc aactggtttt gatgacaaac caacatcaca tggtcgtaaa tctggtagca
    16621 ctggattcgg aacaagcgta aatacttcta gcaatgcaac ttctaaagat gaatcattca
    16681 cttcaaattc atcaaatgca caagcaactg atagtgtaag tgaaagaaca catacaacta
    16741 aagaagatga tattcctagc ttcattagaa atagagaaga aagacgttca agaagaacaa
    16801 gacgttaatc ggttaatata tatacacaaa taattcaaca caaatcatca gataacatat
    16861 ctgatgattt ttttactaat ttttagaaca tgtagaagga catttaagtt tttcaaagtt
    16921 aataaaagtg tttaagtatc gtgtcaaaat taagtcaaaa attatttgcg caacatttta
    16981 actttaaaca taaatgttat attatataat tattaacttt gtacagttag acgaagataa
    17041 tttaaatgaa atgatggtga cgatcgagtg aatgataatt ttaaaaagca accgcatcat
    17101 ttaatatatg aagaattatt acaacaaggt attactctag gtattacaac tagaggcgat
    17161 ggtttaagtg actatcctaa aaatgctttt aatatggcga gatatattga tgatcgccca
    17221 tataatatta ctcaacatca attgcaatta gctgaagaaa ttgcgtttga tagaaaaaat
    17281 tgggtgtttc ccatccaaac acatgaaaat aaagtcgctt gtattacaaa ggatgatata
    17341 ggcacaaata tagacacttt aactgatgcg cttcatggta ttgatgcgat gtacacatat
    17401 gatagtaatg tcttattaac gatgtgttat gcagactgtg taccagtata tttttatagt
    17461 acaaaacatc attttattgc attggcgcat gcaggttggc gtggtaccta tactgaaatt
    17521 gtaaaagaag tgctaaaaca tgtgaacttt gatttgaaag acttacatgt cgttattgga
    17581 ccatctacat catcaagtta tgaaattaat gatgatatta aaaataaatt tgaaacattg
    17641 ccaattgata gtgccaacta tattgaaact agaggacgag atcgtcatgg tattgatttg
    17701 aaaaaagcca atgctgaatt attaaattat tatggtgttc ctaaagaaaa tatttatacg
    17761 acagcgtatg ctacatctga acatttagaa ttatttttct cttatcgatt agaaaaaggt
    17821 caaacaggac gcatgttagc attcattggt caacagtaaa caaggaggag atatgtttgc
    17881 gtgtgaaaga taatttacaa caaatctcaa cacaaattaa tgacaaaagt gaaaaaaata
    17941 atttttcaac aaaaccaaac gtgattgcag ttacaaaata tgttacaata gagcgagcta
    18001 aagaagcgta tgaggctgga ataagacatt ttggtgagaa tagattggaa ggcttttttc
    18061 aaaagaaaga agcattacca tcagatgcgg tgattcattt tataggatca ttacaatctc
    18121 gaaaagttaa ggacgttata aacgacgtag attatttcca tgctttagat cgattgagct
    18181 tagccaaaga aattaacaaa cgtgcagaac ataaaattaa atgtttcttg caagtgaacg
    18241 tttcgggaga agcttctaaa catggtattg ctttagaaga tgttgatcag tttatagatg
    18301 atcttaaaaa atatgacaaa atcgaaattg taggtttaat gacgatggca ccattgacag
    18361 atgatgaagc atatattaga tcgttattta aacagttacg tttgaaaaaa gaagaaatac
    18421 aacgactcaa tttagaatat gcgccttgtg atgaattatc aatgggaatg agtaatgact
    18481 atcttattgc agttgaagaa ggtgcgacgt ttgttagaat tgggactaaa cttgtaggag
    18541 aagaggagtg agccacttgg ctttaaaaga tttatttagt ggattttttg taatagatga
    18601 tgaagaggaa gtagaagtac ctgacaaaca acaacaggtc aatgaagcgc cagcaaaaga
    18661 gcagtcacaa caaacaacaa aacaaaacgc aatcaaatca gtccctcaaa aatctgcatc
    18721 aagatataca acaacgtcag aagaaaggaa taaccgtatg tctaattatt caaaaaataa
    18781 ttcacgtaat gttgtaacta tgaacaatgc tacaccaaac aatgcatcac aagaaagttc
    18841 aaaaatgtgt ttattcgaac cacgtgtttt ctcagataca caagatattg ctgatgagct
    18901 taaaaaccgc cgtgcgacac ttgtcaattt acaacgtatt gataaagtat cagcgaaaag
    18961 aattattgat tttttaagcg gtactgttta tgcaatcggt ggagatatcc aacgtgtagg
    19021 tactgatatt ttcttatgta cgcctgataa tgtggaagta gctggaagca ttacagacca
    19081 tattgaaaat atggaacatt cattcgacta aggagttaac atatggatat aaatgtgcta
    19141 gctacaatat ttaaatttat cctttttgtt gttgaaattt attatttcgg catgattata
    19201 tatttcttta catcttgggt accaagtatt agagaaacta aggtaggtta tttattagcg
    19261 aaaatatatg aacctttctt acaaccattt agaaaagtaa ttccacctat tggaattatc
    19321 gacatatcat caatcgctgc aattatcgtt ttagtattat tccaaaaagg gttactccaa
    19381 atctttaatt ggattttaat tcaattacaa taaattaagc gacataacga ggcaagggta
    19441 catgatactt tagcctcgtt tttgatatgt atttttctga atataagggc aatagatggt
    19501 attttataaa ttttttaagg tagtgattaa catagatatt tatcaacact ttaggcagga
    19561 agaatacgaa ttaattgatc agctaacgga taaatgtgat caagcggaac accattatgc
    19621 accagtatta acgcattttt tagatccaag agggcaatat atattggaag tgatttgtgg
    19681 cagttatgaa gatttaaacg tatcttttta tggcgggcct aatgctgaaa gaaaaagagc
    19741 aatcatttcg ccgaactatt atgaacctaa agaaagtgac tttgaattaa ctttaatgga
    19801 aatagagtat cctgaaaaat tcgtcacttt aaaacatcaa catattttag ggacattaat
    19861 gtctttaggt atcgaacgcg aacaagttgg agatataatt gtgaatgaac gaattcaatt
    19921 tgttttgaca agtagattgg aatcatttat tatgttagaa ttacaacgta ttaaaggcgc
    19981 atcagttaaa ctttatacta ttccagtaac agatatgata caatctaatg agaattggaa
    20041 aaatgaaagt gcaacagtta gttctttaag gttagatgtt gttattaaag aaatgatacg
    20101 taaatcacgt acgattgcga aacaactaat cgaaaaaaaa cgtgttaaag tgaatcacac
    20161 tattgttgat tcagcagatt ttcaattaca agcaaatgat ttaatatcca tccaaggttt
    20221 tggtagagca cacattactg acttaggtgg taaaactaaa aaagataaaa cgcacattac
    20281 ctatagaaca ttattcaaat agtaatgatt taaggaggat aacaaatgcc ttttacacca
    20341 aatgaaatta agaataaaga gttttcacgt gtaaagaatg gtttagaacc tactgaagtt
    20401 gctaattttt tggagcaact aagcactgaa attgaacgtc ttaaagaaga taaaaaacaa
    20461 cttgaaaaag taatcgaaga gagagatact aatattaagt cttatcaaga cgtgcatcaa
    20521 tctgtaagtg atgctttgat acaagcccaa aaagctggtg aagaaactaa acaagctgca
    20581 gagaaacaag ctgaagcgat tatagctaag gcagaagcgc aagctaatca aatggttggt
    20641 gacgcggtag aaaaagcacg ccgtttagca ttccagactg aagatatgaa acgtcaatca
    20701 aaagtattta gatcgcgttt ccgtatgtta gttgaagcgc aattagactt attaaaaaac
    20761 gaagattggg attacttgtt gaattatgat ttagacgctg aacaagtgac gcttgaaaat
    20821 attcatcatt tgcatgaaaa tgatttaaag ccagatgaag ttgcagcaaa tgcacaaaat
    20881 aatgcatcaa atacaccaga caataatcaa caatccaatg attcagaaac aactaagaag
    20941 taagaattaa ataaagacag acgcgtaata tacatttaac ttttcacagc gaattaggta
    21001 atggtgagag cctagtaaaa gcatgtatgt tatatcactg gctttttaat atttaaataa
    21061 tgtaatgaga gaactctaag ttgagttaat aagggtggta ccgcgagcaa tcgtcccttt
    21121 taatttaact tagagttttt taaattttta aggagtgaaa aaaatggatt acaaagaaac
    21181 gttattaatg cctaaaacag atttcccaat gcgaggtggt ttaccaaaca aggagccgca
    21241 aattcaagaa aaatgggatg cagaagatca ataccataaa gcgttagaaa aaaataaagg
    21301 taacgaaaca ttcattttac atgatggccc accatacgcg aatggtaact tacatatggg
    21361 acatgccttg aacaaaattt taaaagactt tattgtacgt tataaaacta tgcaagggtt
    21421 ctatgcacca tacgtaccag gttgggatac acatggttta ccaattgaac aagcattaac
    21481 gaaaaaaggt gttgaccgaa agaaaatgtc aacagctgaa ttccgtgaga aatgtaaaga
    21541 atttgcttta gaacaaattg aattacagaa aaaagatttt agacgtttag gtgttcgtgg
    21601 taactttaat aatccatata tcacattaaa acctgaatac gaagctgcac aaattcgtat
    21661 ttttggagaa atggcagata aaggtttaat ttataaaggt aaaaagccag tttattggtc
    21721 tccttcaagt gagtcttcat tagcagaagc agaaattgaa tatcacgata aacgttcagc
    21781 atcaatttac gttgcatttg acgttaaaga tgacaaaggt gtcgttgatg cagatgctaa
    21841 atttattatc tggacaacaa cgccatggac aattccatca aatgttgcga ttaccgttca
    21901 tcctgaatta aaatatggtc aatacaatgt aaatggcgaa aaatatatta ttgcagaagc
    21961 cttatctgac gctgtagcag aagcactgga ttgggataaa gcatcaatca aattagaaaa
    22021 agaatacaca ggtaaagaat tagagtatgt tgtagcacaa catccattct tagacagaga
    22081 atcgttagtg attaatggtg atcatgttac tacagatgct ggtacaggtt gtgtacatac
    22141 agcaccaggc cacggggaag atgactatat tgttggtcaa aaatatgaat taccagtaat
    22201 tagtccaatc gatgataaag gtgtatttac tgaagaaggc ggccaatttg aaggaatgtt
    22261 ctatgacaaa gctaataaag ccgttactga tttattaaca gaaaaaggtg cactattaaa
    22321 attagacttt attacacata gctatccaca cgactggaga acaaaaaaac ctgtaatctt
    22381 ccgtgctaca ccacaatggt ttgcttcaat cagtaaagta agacaagata ttttagatgc
    22441 aatcgaaaat acaaacttca aagtaaattg gggtaaaaca cgtatttaca atatggttcg
    22501 tgaccgtggc gaatgggtta tttctcgtca acgtgtttgg ggtgtaccgt taccagtatt
    22561 ttatgctgaa aatggcgaaa ttatcatgac gaaagaaaca gtgaatcatg ttgctgattt
    22621 atttgcagaa cacggttcaa atatttggtt tgaaagagaa gcgaaagact tactaccaga
    22681 aggatttaca catccaggca gccctaacgg tacatttact aaagaaacag acattatgga
    22741 cgtttggttt gattctggtt catcacaccg tggcgtgttg gaaacaagac cggaattaag
    22801 tttcccagca gatatgtatt tagaaggtag tgaccaatat cgtggttggt tcaactcttc
    22861 tatcacaact tcagttgcta caagaggagt atcaccttat aaattcttac tttctcatgg
    22921 ttttgttatg gatggtgaag gtaagaaaat gagtaaatct ttaggtaatg tgattgtacc
    22981 tgaccaagtg gttaaacaaa aaggtgctga tattgcgaga ctttgggtaa gtagtacgga
    23041 ctatttagct gatgttagaa tttctgatga aattttaaaa caaacatctg atgtttatcg
    23101 taaaatcaga aatacattaa gatttatgtt aggtaacatt aacgatttca atcctgacac
    23161 agatagcatt cctgaatcag agttattaga agtggatcgt tacttgctaa atcgtttacg
    23221 tgaatttact gcaagtacga ttaacaacta tgaaaacttt gactacttaa atatttatca
    23281 agaagttcaa aactttatca atgttgagtt aagtaatttc tatttggatt acggtaaaga
    23341 cattttatat attgaacaac gtgattctca tatccgtcgc agtatgcaaa cagtgttata
    23401 tcaaatttta gttgatatga cgaagttgtt agcaccaatc ttagtgcata cagctgaaga
    23461 agtttggtct catacaccac atgttaaaga agaaagtgtt cacttagcag acatgcctaa
    23521 agttgtagaa gtagatcaag ctttattgga taaatggcgt acatttatga atttacgtga
    23581 tgatgtgaat cgtgcattag aaactgctcg taatgaaaaa gttattggta aatcattaga
    23641 agctaaagtt acgattgcta gtaacgataa atttaatgca tctgaattct taacttcatt
    23701 tgatgcatta catcaattat ttatcgtgtc acaagttaaa gttgtagata agttagatga
    23761 tcaggcaaca gcttatgaac atggtgatat tgtcatcgaa catgcagatg gtgaaaaatg
    23821 tgaaagatgt tggaactatt cagaggatct tggtgctgtt gatgaattga cgcatctatg
    23881 tccacgatgc caacaagttg taaaatcact tgtataattg aaattgtata aagtactcat
    23941 acagatgata taaattaaag ctctcttcat aatcatgttg tagtttttgt tgacatgatg
    24001 aagggagttt ttttacgaat aaaaaaatga ccaagttact ggttatatgt gttaaaaaat
    24061 gtgtaattta ctaaaataaa aattattcag gaatggtaca aattctctaa ggcttataaa
    24121 tgcgttatag ttgctattct caattatgtt cgcgataatt ttaagtaaaa gtaagcacag
    24181 atattgaatt tgataggagt taattgaatg tatcataaca gtaacgcaaa ctttgtaaat
    24241 ggtatcactt taaatgtaag agataagaat gaattaaagc cattttatga ggacgtatta
    24301 ggattaaata ttataaatga gacattaaca tcgatacaat atgaagtagg ccaaaataat
    24361 catgtcatta cacttgttga attacaaaat ggacgtgaac ctttaatgtc cgaagcggga
    24421 ctgtttcata tcgcaattaa actacctcaa attagtgatt tagctaattt actaattcat
    24481 ttaagcgaat atgatattcc agttaacggt ggtatacagc ctgcttcgtt atcattattt
    24541 tttgaagacc cggaaggaaa cggttttaaa ttttatgttg ataaagacga agcgcaatgg
    24601 acgaggcaaa atgatttagt aaaaattgat attagaccat taaatgtacc gagattagtg
    24661 agtcatgcaa caaaattgtt atggttaggt attccagatg acgctattat aggtgcattg
    24721 catattaaga caattcattt atcagaggta aaagagtact acctcgatta ttttggatta
    24781 gagcaatcgg catatatgga tgattattca atatttttag catcgaatgg ctattatcaa
    24841 catttggcca tgaatgattg ggtatcagca acgaaacgtg tagaaaattt tgatacgtat
    24901 ggattagcaa ttgttgactt tcattatctt gaaacaacac atttaaattt acaaggtccg
    24961 gatggtatct attatcgctt taatcatatc gaagttgaag gttagtatat gctttgaatg
    25021 gacgaaccat ataatgaatc gtttttaatg atctttttat acaagctatg aaggaggctg
//
LOCUS       DAHHOJ010000031        23907 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.031, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000031 DAHHOJ010000000
VERSION     DAHHOJ010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23907)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23907)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23907
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.031"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            90..1016
                     /gene="hemC"
                     /locus_tag="NBH32_001063"
     CDS             90..1016
                     /gene="hemC"
                     /locus_tag="NBH32_001063"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCD1514681.1"
                     /translation="MRKLVVGSRRSKLALTQSQQFIDKLKAVEPNLEIEIKEIVTKGD
                     RIVDKQLSKVGGKGLFVKEIQHELFEKNIDMAIHSLKDVPSVIPEGLTLGCIPDRELP
                     FDAYISKTHTPLSQLPEGSIIGTSSLRRGAQILSKYPNLEIKWIRGNIDTRLEKLQTE
                     DYDAIILAAAGLRRMGWSDDIVTSYLDRDTLLPAIGQGALGIECRSDDEELLTLLSKV
                     HNDEVAKCVTAERTFLAEMDGSCQVPIAGYATISDQKEIEFTGLIMTPDGKERFEYTM
                     NGTDPVELGKTVSNKLKEQGAYEIIKRLNEQH"
     gene            1038..1706
                     /locus_tag="NBH32_001064"
     CDS             1038..1706
                     /locus_tag="NBH32_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCD1514682.1"
                     /translation="MKPVVVMTQTNDMQSDLVSIIHKPFIDIKPLNFDIHLLNQRYDW
                     LVFSSKNAVKFFYKYLKGINVDNIAVIGSKTAQYCESLGIRVDFMPNDFSQEGFLKSF
                     NQTNQKILLPSSELARPLLLAALSKNNEVVKIDLYTSVPNKQNIQDVKEMIEHQQIDA
                     LTFSSSSAVRYYFNEGFVPKFKSYFAIGEQTARTIKSYQQPVTIAEIQTLESLIEKIL
                     ESRG"
     gene            1709..2683
                     /gene="hemB"
                     /locus_tag="NBH32_001065"
     CDS             1709..2683
                     /gene="hemB"
                     /locus_tag="NBH32_001065"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001633925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCD1514683.1"
                     /translation="MKFDRHRRLRSSATMRDMVRENHVRKEDLIYPIFVVEKDDVKKE
                     IKSLPGVYQISLNLLESELKEAYDLGIRAIMFFGVPNSKDDIGTGAYIHDGVIQQATR
                     IAKKMYDDLLIVADTCLCEYTDHGHCGVIDDHTHDVDNDKSLPLLVKTAISQVEAGAD
                     IIAPSNMMDGFVAEIRRGLDEAGYYNIPIMSYGVKYASSFFGPFRDAADSAPSFGDRK
                     TYQMDPANRLEALRELESDLKEGCDMMIVKPALSYLDIVRDVKNHTNVPVVAYNVSGE
                     YSMTKAAAQNGWIDEERVVMEQMVSMKRAGADMIITYFAKDICRYLDK"
     gene            2731..4017
                     /gene="hemL"
                     /locus_tag="NBH32_001066"
     CDS             2731..4017
                     /gene="hemL"
                     /locus_tag="NBH32_001066"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD1514684.1"
                     /translation="MRYTKSEEAMKVAETLMPGGVNSPVRAFKSVDTPAIFMDHGKGS
                     KIYDIDGNEYIDYVLSWGPLILGHRDPQVISHLHEAIDKGTSFGASTLLENKLAQLVI
                     DRVPSIEKVRMVSSGTEATLDTLRLARGYTGRNKIVKFEGCYHGHSDSLLIKAGSGVA
                     TLGLPDSPGVPEGIAKNTITVPYNDLDALKIAFEKFGDDIAGVIVEPVAGNMGVVPPI
                     EGFLQGLRDITTEYGALLIFDEVMTGFRVGYHCAQGHFGVTPDLTCLGKVIGGGLPVG
                     AFGGKKEIMDHIAPLGNIYQAGTLSGNPLAMTSGYETLSQLTPETYEYFNMLGDILED
                     GLKRVFAKHNVPITVNRAGSMIGYFLNEGPVTNFEQANKSDLKLFAEMYREMAKEGVF
                     LPPSQFEGTFLSTAHTKEDIEKTIQAFDTALSRIVK"
     gene            4078..4179
                     /locus_tag="NBH32_001067"
     CDS             4078..4179
                     /locus_tag="NBH32_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514685.1"
                     /translation="MGGDLIVKVHICFVVKTASGYCYLNKREAQAAI"
     gene            4213..5286
                     /locus_tag="NBH32_001068"
     CDS             4213..5286
                     /locus_tag="NBH32_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCD1514686.1"
                     /translation="MTMIYRNNFIVFVLSFFISIILYSSHVLLPFMFGPIIASIICVK
                     VFKLDIKWPFLLSELGIVLLGVQIGSTFTKNVVMDIKDNWLSIIVVSISILLIALVMA
                     FIFKKIARINTETAILSVIPGALTQMLVMAEQDKRANLLVVSLTQTSRIIFVVVLVPF
                     ISYFFHDGNMHANGKLTKVLPLSQVLNIGQIVILAIAIFIVYLIMSKIKFPTFQLLAP
                     LIVLIVWNFSTDLTFTLDHWLLNMAQLIYMIRIGVQIAHLLSDLKGRLAIAITIQNIM
                     LIIGALIMVYVIHFFDNNPINELFLGAAPGGVNQIVLVALATGADVAMITSYHIFRIF
                     FILFIIAPGINYFLKYRSNKRED"
     gene            complement(5509..6069)
                     /locus_tag="NBH32_001069"
     CDS             complement(5509..6069)
                     /locus_tag="NBH32_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCD1514687.1"
                     /translation="MNDCAFGTKDPVYLDYHDYVWGQPLYDSKALFKLLALESQHAGL
                     SWLTILKKKEAYEEAFYDFEPEKVAQMTAQDIDRLMTFPNIVHHRKKLEAIVNQAQGY
                     LKIEQAYGSFSKFLWSYVNGKPKDLQYEHASDRITVDDTATQLSKDLKQYGFKFLGPV
                     TVFSFLEAAGLYDAHLKDCPSKPKHN"
     misc_binding    6127..6428
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            6473..9103
                     /locus_tag="NBH32_001070"
     CDS             6473..9103
                     /locus_tag="NBH32_001070"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCD1514688.1"
                     /translation="MEMKPKYDPREVEAGRYEEWVKNGYFKPSEDKSKETYTIVIPPP
                     NVTGKLHLGHAWDTTLQDIITRMKRMQGYDTLYLPGMDHAGIATQAKVEAKLNEQGIT
                     RYDLGREKFLEQAWDWKEEYASFIRAQWAKLGLGLDYSRERFTLDEGLSKAVKKVFVD
                     LYNKGIIYRGERIINWDPKARTALSDIEVIHEDVQGAFYHFKYPYADGEGFIEIATTR
                     PETMLGDTAIVVNPNDERYKDVIGKTVILPIVGRELPILADEYVDIDFGSGAMKVTPA
                     HDPNDFEIGQRHQLENIIVMDENGKMNDKAGKYEGMDRFDCRKQLVEDLKEQDLVIKI
                     EDHVHSVGHSERSGAVVEPYLSTQWFVRMEDLAKRSLDNQKTDDRIDFYPQRFEHTFN
                     QWMENIRDWTISRQLWWGHQIPAWYHKETGEIYVGEEAPTDIENWQQDEDVLDTWFSS
                     ALWPFSTLGWPDLESEDFKRYYPTNALVTGYDIIFFWVARMIFQGLEFTDRRPFNDVL
                     LHGLVRAEDGRKMSKSLGNGVDPMDVIDEYGADSLRYFLATGSSPGHDLRYSTEKVES
                     VWNFINKIWNGARFSLMNIGEDFKVEDIDLSGNLSLADKWILTRLNETIATVTDLSDK
                     YEFGEVGRALYNFIWDDFCDWYIEMSKIPMNGNDEEQKQVTRSVLSYTLDNIMRMLHP
                     FMPFVTEKIWQSLPHEGDTIVKASWPEVRESLIFEESKQTMQQLVEIIKSVRQSRVEV
                     NTPLSKEIPILIQAKDKEIETTLSQNKDYLIKFCNPSTLNISTDVEIPEKAMTSVVIA
                     GKVVLPLEGLIDMDKEISRLEKELAKLQSELDRVDKKLSNENFVSKAPEKVINEEKRK
                     KQDYQEKYDGVKARIEQLKA"
     gene            9116..10387
                     /locus_tag="NBH32_001071"
     CDS             9116..10387
                     /locus_tag="NBH32_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCD1514689.1"
                     /translation="MNYLESLYWIHERTKFGIKPGVKRMEWMLAQFNNPQNNIKGIHV
                     GGTNGKGSTVAYLRTALVENGYEVGTFTSPFIETFNERISLNGVPISNDAIVELVSRI
                     KPVSEMMERETDLGVATEFEIITAMMFLYFGEIHPVDFVIVEAGLGIKNDSTNVFTPV
                     LSILTSIGLDHTDILGGTYLDIARDKGAIIKPNVPVIYAVKNEDALKYVRERAIEQHA
                     KPIELDREIVVVSQNDEFTYRYKDYELETIILSMLGEHQKQNAALAITALIELNEQGL
                     IELDFNKMIDGIESVRWTGRIEQVHDKPLIILDGAHNSESIDALIDTIKQYHDKEKVD
                     ILFSAINGKPINEMVKHLSLIAHTFYATEFDFPKALRKEEIVGSIENDEIQLVDDYVE
                     FIKNYQGDTLVITGSLYFISEVKSKLDFEAL"
     gene            10657..11364
                     /locus_tag="NBH32_001072"
     CDS             10657..11364
                     /locus_tag="NBH32_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCD1514690.1"
                     /translation="MVVLLSYSCSCIFSFLYQFISIEETSFDYLHRRSKCDYCNSSLK
                     WYELMPIISFLLLKGRCRNCRKRISLTHFLGETFAIIPIVFIKYDFTYVNATLFITTY
                     VFLLIFTMTDITSLMLDCRLIIIYCIVSLSLSMIYPVAFIIISMTTHIFYFLFRAYIG
                     YGDVLLISALSLFFPLQFTIYVILFTFVIAGLVALITMIFKPIKLLPLVPFIFISFFI
                     NSLFYNDIHQFLGGVYF"
     gene            11361..12047
                     /gene="radC"
                     /locus_tag="NBH32_001073"
     CDS             11361..12047
                     /gene="radC"
                     /locus_tag="NBH32_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000692885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCD1514691.1"
                     /translation="MKIKEMVTSEMPRERLLSHGAKSLSNTELLAILINTGRKGFSSI
                     DISNELLKSASNLNELKKSSINDLIQVKGIGLQKAITLKAAFELGERMGRRAENNRIK
                     ITQPSDVADYMIPTMKDLTQEHFVILLLNSKNVVIKETCVFKGTLNSSIVHPREIFSI
                     AVRENANAIIAVHNHPSGDVTPSQEDIITTMRLKECGLILGIDLLDHIIIGDNRFTSL
                     VEAGYFDEND"
     gene            12115..12399
                     /locus_tag="NBH32_001074"
     CDS             12115..12399
                     /locus_tag="NBH32_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514692.1"
                     /translation="MTLFIIIGVLVPMVYTMQLNIKNEPVTKRNLLITLALSTLGILV
                     TALAGVIVTKQAFPLLSVAIGSIFTGIVWGLLLSGSYALIRFLSNAFGRK"
     gene            complement(12516..12713)
                     /locus_tag="NBH32_001075"
     CDS             complement(12516..12713)
                     /locus_tag="NBH32_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514693.1"
                     /translation="MSILTIILIALLVILLFRVGLSILRFLIYVGLVLLCIYLGYQGL
                     IWLLDFFQINSGFLPHFQFNN"
     gene            12873..13346
                     /locus_tag="NBH32_001076"
     CDS             12873..13346
                     /locus_tag="NBH32_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4930 family protein"
                     /protein_id="HCD1514694.1"
                     /translation="MRLIFSMIKNIIAVIAIVIIVYIALKYAPFLKEQDWNPVNHDND
                     QMNQVTQPTNDAQHVYVSGEKYSLKENDLIKNVPLSQIKNVFKMIDKQEFMAVSGMNR
                     MAYNDQYIIGQRGDEFILYKFGDESMRVYNTEFEMQQDLNELGQNLQLKPENAYQ"
     gene            13420..13539
                     /locus_tag="NBH32_001077"
     CDS             13420..13539
                     /locus_tag="NBH32_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514695.1"
                     /translation="MAEQSKQKQANEQQKAQNLFARWRQLQNSNSESSNDTNK"
     gene            13739..14581
                     /gene="mreC"
                     /locus_tag="NBH32_001078"
     CDS             13739..14581
                     /gene="mreC"
                     /locus_tag="NBH32_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCD1514696.1"
                     /translation="MLKFFKNNKLIVVLCAIIVFIALIGLSIRSQSQSPPEQYIGDSV
                     SFGQRVVSYPVNFVAGTIGDFFKKGDSKESKNKISQLESKNQQLEAENEKLKKELDLK
                     DISKFDPISTTVLARNPDQWMNTIVIDKGSKSGITSNMAVMTSKGFVGRVTKVNKFSS
                     QVDLISTNTRAGKLSVNIQHGSKNIFGLIDRYDEKNSELVISDINNRDNISKGDKVVT
                     SGLADQLPSDLYIGEVTKVQNDQYGLAKEVRIKTGADLTDLSHVYVAKRDPKTIPDDE
                     SRDK"
     gene            14581..15111
                     /gene="mreD"
                     /locus_tag="NBH32_001079"
     CDS             14581..15111
                     /gene="mreD"
                     /locus_tag="NBH32_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCD1514697.1"
                     /translation="MRTLYYFLIGILLFYIDTAIGLLIPMNIGKFELVFVPHLTFMYI
                     LMMVVYRGFGVSLLLSIFLGVMTDVYFGSIYGVYLFGYILFLALIDRFFKIFYKDHSM
                     LFIIILASTLLLEVYVALIYGMLGFIQFDIIHFVVFRLLPTLIMNFVLLIMLYPLIIK
                     FLKKTNNDIDMKRRQW"
     misc_feature    15138..15334
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            15347..15655
                     /gene="rplU"
                     /locus_tag="NBH32_001080"
     CDS             15347..15655
                     /gene="rplU"
                     /locus_tag="NBH32_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000457386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCD1514698.1"
                     /translation="MFAIIETGGKQIKVEEGQEIFVEKLDVNEGDTFTFDKVLFVGGD
                     SVKVGAPTVEGATVTATVNKQGRGKKITVFTYKRRKNSKRKKGHRQPYTKLTIDKINA
                     "
     gene            15661..15981
                     /locus_tag="NBH32_001081"
     CDS             15661..15981
                     /locus_tag="NBH32_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HCD1514699.1"
                     /translation="MITVDITVNDEGKVTDVIMDGHADHGEYGHDIVCAGASAVLFGS
                     VNAIIGLTSERPDINYDDNGGHFHIRSVDTNNDEAQLILQTMLVSLQTIEEEYNENIR
                     LNYK"
     gene            15993..16277
                     /gene="rpmA"
                     /locus_tag="NBH32_001082"
     CDS             15993..16277
                     /gene="rpmA"
                     /locus_tag="NBH32_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCD1514700.1"
                     /translation="MLKLNLQFFASKKGVSSTKNGRDSESKRLGAKRADGQFVTGGSI
                     LYRQRGTKIYPGENVGRGGDDTLFAKIDGVVKFERKGRDKKQVSVYAVAE"
     gene            16497..17789
                     /gene="obgE"
                     /locus_tag="NBH32_001083"
     CDS             16497..17789
                     /gene="obgE"
                     /locus_tag="NBH32_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020444729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HCD1514701.1"
                     /translation="MFVDQVKISLKAGDGGNGITAYRREKYVPFGGPAGGDGGKGASV
                     VFEVDEGLRTLLDFRYQRHFKASKGENGQSSNMHGKNAEDLVLKVPPGTIIKNVETDE
                     VLADLVEDGQRAVVAKGGRGGRGNSRFATPRNPAPDFSEKGEPGEELDVSLELKLLAD
                     VGLVGFPSVGKSTLLSIVSKAKPKIGAYHFTTIKPNLGVVSTPDQRSFVMADLPGLIE
                     GASDGVGLGHQFLRHVERTKVIVHMIDMSGSEGREPIEDYKVINQELAAYEQRLEDRP
                     QIVVANKMDLPESQDNLNLFKEEIGEDVPVIPVSTITRDNIDQLLYAIADKLEEYKDV
                     DFTVEEEESVGINRVLYKHTPSQDKFTISRDDDGAYVVSGNAIERMFKMTDFNSDPAV
                     RRFARQMRSMGIDDALRERGCKNGDIVRILGGEFEFVE"
     gene            17802..18257
                     /locus_tag="NBH32_001084"
     CDS             17802..18257
                     /locus_tag="NBH32_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD1514702.1"
                     /translation="MDNKDYKKFYLIREDVLPESVVKTLKIKDALKSDPTLSIYDAVK
                     QFDLSRSAFYKYRETIFPVDDKMLDHREFTLILYVTDIVGMLARVLDVISKLELSVLT
                     IHQSIPMEEKATITLSLNAKSKETAVEDVIGALRNLDYVSKVELISMSM"
     gene            18271..18873
                     /gene="ruvA"
                     /locus_tag="NBH32_001085"
     CDS             18271..18873
                     /gene="ruvA"
                     /locus_tag="NBH32_001085"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCD1514703.1"
                     /translation="MYAYVKGKLTHLYPTHVVVETAGVGYEIQTPNSYRFQKQLDHEV
                     LIHTSLIVREDAQLLYGFSSEEEKDMFLSLIKVTGIGPKSALAILATSTPNEVKRAIE
                     NENDTYLTKFPGIGKKTARQIVLDLKGKVKITEEDSDSLLQVDATSTVQDQFVQEAML
                     ALEALGYSKRELAKVEKTLNKNKYDSVDEAVKAGLQLVVS"
     gene            18905..19909
                     /gene="ruvB"
                     /locus_tag="NBH32_001086"
     CDS             18905..19909
                     /gene="ruvB"
                     /locus_tag="NBH32_001086"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCD1514704.1"
                     /translation="MNERMVDQSMHSEETDFELSLRPTRLRQYIGQNSIKSNLEVFIK
                     AAKLRHEPLDHVLLFGPPGLGKTTLSNIIANEMEVNIRTVSGPSLERPGDLAAILSGL
                     QPGDVLFIDEIHRLSSVVEEVLYPAMEDFFLDIIIGKGDEARSIRIDLPPFTLVGATT
                     RAGSLTGPLRDRFGVHLRLEYYNESDLKEIIIRTAEVLGTGIDEESAIELAKRSRGTP
                     RVANRLLKRVRDFQQVNEDEQIYIETTKHALGLLQVDQHGLDYIDHKMMNCIIKQYNG
                     GPVGLDTIAVTIGEERITIEDVYEPFLIQKGFLERTPRGRKATPLAYEHFAKSNEERE
                     "
     gene            19911..20936
                     /gene="queA"
                     /locus_tag="NBH32_001087"
     CDS             19911..20936
                     /gene="queA"
                     /locus_tag="NBH32_001087"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCD1514705.1"
                     /translation="MNFEEFDYDLPESLIAQTPLKDRDHSRLLVMDRETGEMKHLHFK
                     DIIEYFRPGDTLVLNDTRVMPARLFGLKEETGAKVEMLMLTQIEGNDWEVLLKPAKRI
                     KVGNKLNFGNGKIIAECIKEMDQGGRIMRLHYEGILQERLDELGEMPLPPYIKERLDD
                     PDRYQTVYAKESGSAAAPTAGLHFTDELLTEIKNKGVNIAFVTLHVGLGTFRPVSVDD
                     VNDHEMHSEYYQMTQETADLLNDTESKGHRIISVGTTSTRTLETIRRDHDKFVETSGW
                     TNIFIYPGFDFKAIDGQITNFHLPKSTLVMLVSAFSTRENVLNAYKTAVNLEYRFFSF
                     GDAMLII"
     gene            20959..22098
                     /gene="tgt"
                     /locus_tag="NBH32_001088"
     CDS             20959..22098
                     /gene="tgt"
                     /locus_tag="NBH32_001088"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016425271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCD1514706.1"
                     /translation="MPAVTYEHIKTCKQSGARLGIVHTPHGSFETPMFMPVGTKATVK
                     TMSPEELRQIEAKIILGNTYHLWLQPGNDIIKHAGGLHKFMNWDGPILTDSGGFQVFS
                     LSNLRKITEEGVEFRHHTNGSKLFLSPEKSMQIQNDLGSDIMMAFDECPPMPAEYDYV
                     KKSIERTTRWAKRCLDAHQRPEDQALFGIIQGGEYEDLREQSAKDLVELDFPGYAIGG
                     LSVGEPKPVMYKMVEHTEQFMPKDKPRYLMGVGSPDALIECSIRGMDMFDCVLPTRIA
                     RNGTCMTSQGRLVIKNAKFADDLRPLDENCDCYTCQNYSRAYIRHLIKAEETFGIRLT
                     TIHNLHFLLKLMEDIRQAIREDRLLDFKEEFFEQYGLNVENPKNF"
     gene            22117..22377
                     /gene="yajC"
                     /locus_tag="NBH32_001089"
     CDS             22117..22377
                     /gene="yajC"
                     /locus_tag="NBH32_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCD1514707.1"
                     /translation="MQFSLLIYIVVIFAVMYFLMIRPQQKRAKQHRELINNIQSGQRI
                     TTIGGIKGTVKAVDETTVVITVNGHGTELTFEKPAIKQVDPS"
     gene            22652..>23907
                     /gene="secD"
                     /locus_tag="NBH32_001090"
     CDS             22652..>23907
                     /gene="secD"
                     /locus_tag="NBH32_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCD1514708.1"
                     /translation="MKKSSRIIAFLLLVVLLFAGMAATYKSVIKNVNLGLDLQGGFEV
                     LYQVDPLNKGDKIDKKALQSTAQTLENRVNVLGVSEPKIQVEEPNRIRVQLASVTDQN
                     EARKILSSQANLTIRDAEDKVKLSGSDIKQGSAKQEFKQETNQPTVTFKVKDKNKFKK
                     VTEEISKKRDNVMVVWLDFKKGDSYKKEAQKKNPKFISAASVDQPINSDSVEISGGFK
                     GQEGVKKAKQIAELLNAGSLPVDLKEIYSNSVGAQFGQDALDKTVFASFIGVALIYLF
                     MLGFYRLPGLVAIIALTTYIYLTLVAFNFISGVLTLPGLAALVLGVGMAVDANIIMYE
                     RIKDELRIGRTIKQAFSKANKSSFLTIFDSNLTTVIAAAVLFFFGESSVKGFATMLLL
                     GILMIFVTAVFLSRFLLSLLVSSNIF"
BASE COUNT         8367 a         3282 c         4615 g         7643 t
ORIGIN      
        1 tgcttaaata tgattaattt attttttgca acacattttg tgaattgaga aaataatcta
       61 gagcaatccg ttcctaggag gaattgaaca tgcgtaaatt agtcgttggc tccagaagaa
      121 gtaaattagc tttaacacaa agccagcaat ttattgataa attaaaagct gtcgagccaa
      181 atctagaaat tgaaattaaa gaaattgtca cgaaaggcga tcgtatagta gataaacaat
      241 tgtctaaagt cggaggcaaa ggcttatttg ttaaagaaat acaacatgaa ctttttgaaa
      301 aaaatatcga tatggcaata cactcactta aagacgtacc aagtgtaatt ccggaaggtt
      361 taacattagg ttgtatccct gatagagaat taccttttga tgcgtatatt tctaaaacac
      421 atacaccact atcccaattg ccagaaggca gtattattgg tactagttca ttacgtcgtg
      481 gtgcacaaat attatctaag tatcctaatt tagagattaa atggattaga ggtaatatag
      541 atacacgatt agaaaagtta caaactgaag attatgatgc gattatttta gctgcagctg
      601 gtttaagaag aatgggctgg tcagatgata ttgtaacatc ttatcttgat agagatacat
      661 tgttacctgc aatcggacaa ggtgctttag ggatagaatg tcgtagtgac gatgaagaac
      721 tattaacatt attaagcaaa gtacataatg atgaagttgc aaaatgtgtg actgctgaac
      781 gaacgttttt agcagaaatg gatggtagtt gtcaggtgcc aattgcagga tatgctacaa
      841 tctcagatca aaaagaaatc gaatttacag gtttaattat gaccccagat ggtaaagaac
      901 gatttgaata tacaatgaac ggaacagatc cggttgagtt aggcaaaaca gtgagtaaca
      961 aattaaaaga gcaaggtgct tatgaaatta taaaacgctt aaatgaacaa cattaaataa
     1021 tggagtgacg tttaaatatg aagccagttg tagttatgac acaaacaaat gacatgcaaa
     1081 gtgatttagt atcaattatt cacaaaccct ttattgatat aaagccacta aattttgata
     1141 ttcatttgtt aaatcaacgc tatgactggc ttgttttttc atctaaaaat gctgtgaaat
     1201 tcttttataa atatctaaaa ggaattaatg ttgataacat tgctgtgata ggaagtaaga
     1261 cagcgcaata ttgtgaatca cttggcattc gagttgattt tatgccaaac gacttttctc
     1321 aagaaggatt tttaaaatca tttaatcaaa ctaaccaaaa aatacttttg ccttcgagtg
     1381 aattggcgag accattgtta ttagcagcgt tatctaaaaa taatgaagtt gttaaaatag
     1441 atttatatac ttcagtgcct aacaaacaaa atatacaaga tgttaaagaa atgatagaac
     1501 atcaacaaat cgatgcatta acattttcaa gttcgtcggc agtacgttat tattttaatg
     1561 aaggatttgt accaaaattc aagtcgtatt ttgctattgg agaacaaaca gcacggacca
     1621 ttaaatcata tcaacaacca gtaacaattg cagaaattca aacactcgaa tcactaattg
     1681 aaaagatttt agaaagtagg ggctaaaaat gaaatttgat agacatagaa gattgagatc
     1741 atcagcgaca atgagagata tggttagaga gaatcatgta agaaaagaag atttaatata
     1801 tccaattttt gtagttgaaa aagacgatgt gaaaaaagaa attaagtcat tgccaggtgt
     1861 ataccaaatc agtttgaatt tacttgaaag tgaattaaaa gaagcttatg acttaggcat
     1921 acgtgccatt atgtttttcg gtgttccaaa ctcaaaagat gatataggta ctggtgcata
     1981 cattcacgat ggtgttattc aacaggcgac acgtattgct aaaaaaatgt atgatgactt
     2041 attaattgtt gcagacactt gtttatgtga atatactgat catggtcatt gtggtgtgat
     2101 tgatgaccat acacatgacg ttgacaatga taaatcattg ccactacttg ttaaaacagc
     2161 aatttctcaa gtggaagctg gtgctgatat tattgcgcca agtaatatga tggatggttt
     2221 tgttgctgaa attcgtcgtg gattagatga agccggctat tacaatattc ctataatgag
     2281 ttatggtgtc aagtatgcat caagtttctt tggacctttt agagatgcag cagattcagc
     2341 gccatcattt ggggatagaa aaacgtatca gatggaccct gctaaccgtt tggaagcact
     2401 tcgtgaatta gaaagtgatt taaaagaagg gtgcgacatg atgattgtta aacctgctct
     2461 aagttattta gacatagttc gagatgttaa aaatcatacg aatgttccag ttgttgcata
     2521 taatgtgagt ggagaatata gtatgactaa agcggcggca caaaatggtt ggatagatga
     2581 agaacgtgtc gttatggaac aaatggtttc aatgaaacgt gcaggtgctg atatgattat
     2641 tacgtatttt gcaaaggaca tttgtcgcta tttagataaa taaggtttta tatgtatgat
     2701 tttccataaa ctataggagg aatttacttt atgagatata cgaaatcaga agaagcaatg
     2761 aaggttgctg aaactttaat gcctggtggt gtaaatagtc cagtacgcgc atttaaatca
     2821 gtagatacac cagcaatttt tatggatcac ggtaaaggtt caaaaattta tgatatcgat
     2881 ggtaacgagt atatcgacta tgtactaagt tggggaccac ttattttagg acatagagac
     2941 cctcaagtta ttagtcattt acatgaagca attgataaag gtacaagttt tggtgcatca
     3001 acattacttg aaaataaatt ggcgcagctc gttattgacc gagtaccttc aatagaaaaa
     3061 gtgcgtatgg tgtcatctgg tacagaagct acattggata ctttaagatt agcacgtggt
     3121 tatactggca gaaataaaat tgtgaaattt gaaggttgct atcatggtca tagtgattcg
     3181 ttattaatca aagctggttc tggggtggca acattaggat tgccggattc tcctggtgtg
     3241 cctgaaggta ttgctaaaaa tacaattaca gttccataca atgatttaga tgcacttaaa
     3301 atcgctttcg aaaaatttgg agacgatatt gctggggtaa tcgtagaacc tgttgctggt
     3361 aatatgggtg tcgtaccgcc gattgaaggt tttttacagg gattaagaga tattacgact
     3421 gaatacggag cattgctaat tttcgatgaa gtaatgactg gtttcagagt cggttatcat
     3481 tgtgcacaag gtcactttgg tgtgacacca gatttaactt gcttaggaaa agttatcggt
     3541 ggaggactac ctgtaggtgc ttttggtggt aaaaaagaaa tcatggatca tatagcacca
     3601 ttaggaaata tttatcaagc gggtacgtta tcaggaaatc ctcttgcaat gacaagtggt
     3661 tatgaaacgt taagccaatt aacgccagag acatatgagt attttaatat gttaggcgat
     3721 atacttgaag acggtttaaa gcgtgtattt gctaaacaca atgtaccaat aactgtaaat
     3781 agagcaggtt caatgattgg ttatttctta aatgaaggac ctgtaactaa ttttgaacaa
     3841 gcgaataaaa gtgatttgaa attatttgca gaaatgtatc gagaaatggc aaaagaaggt
     3901 gtgtttttac caccatctca atttgaaggt acattcttat ctacggcaca cacgaaagaa
     3961 gatattgaaa aaacgattca agcatttgat acggctttaa gtcgtattgt aaaataaata
     4021 tacggacaaa ttgagagcct gaactttgtt caggctcttt ttaaatgtat atcagacatg
     4081 ggcggcgact tgatagtgaa agtccatata tgctttgtag tcaaaactgc tagcggatat
     4141 tgttatctta acaaacgtga agctcaagca gcaatttagt cattttattt tttattgaaa
     4201 gaagtggaaa acatgacaat gatatataga aataatttca ttgtgttcgt tttatcattt
     4261 tttattagta ttatattgta ttcatcgcac gtattactcc catttatgtt tggtcctatt
     4321 atcgcatcaa tcatttgtgt gaaagttttc aaacttgata ttaaatggcc attcttactt
     4381 agtgaattag gtattgtact attaggtgtg caaatcggat caacgtttac gaaaaatgtc
     4441 gttatggata ttaaagacaa ttggctttcg attattgttg tatctatttc gatattatta
     4501 attgcattag taatggcatt tatttttaaa aaaattgcac gtattaatac agaaacagct
     4561 attttaagtg ttataccagg agcactaaca caaatgctgg tcatggctga acaagacaaa
     4621 cgtgctaatt tgttagttgt tagcttaacg caaacatcac gaattatatt tgttgttgtt
     4681 ttagtaccgt tcatttcata tttttttcat gatggtaaca tgcatgcgaa tggaaagtta
     4741 acaaaagtct tgcctttatc acaagtatta aacatagggc aaatagttat tttagcgata
     4801 gctatcttta tagtttatct aattatgtct aaaataaagt ttccaacatt tcaattatta
     4861 gcaccactca ttgtattaat tgtttggaat ttttctacag atttaacatt tacactagat
     4921 cattggttgt tgaacatggc acaactaata tatatgatta gaattggagt tcaaattgcg
     4981 catttattgt cagatttaaa aggtagacta gcaatcgcaa ttacaattca aaatattatg
     5041 ttgataattg gtgcgctaat catggtttat gttatacatt tctttgataa taatcctatt
     5101 aatgaattgt ttcttggtgc agcaccagga ggggttaatc agatagtttt agttgcatta
     5161 gctactggtg ccgatgttgc tatgattacg agttatcata ttttccgaat ctttttcatc
     5221 ctatttatta ttgcaccagg aattaattac tttttaaaat atcgttcgaa taagcgtgaa
     5281 gattagaaac tatcatccat ttaagttgaa ttatgcttag gatagaattc attattaagg
     5341 tttaagcaaa ggttgtacat ctatatttag attaagatat ctaatattcg cagtgcatta
     5401 acttggaact ttaacttatg atttgaataa attttcacat caaaaaacga gacagtctat
     5461 tagttaaaaa tagacgatgt ctcgtttatt ttatgatgct atagcaaatt aattgtgttt
     5521 aggctttgat ggacaatctt ttaaatgtgc atcatataaa ccggctgctt ctaaaaacga
     5581 aaatactgtt actggaccta aaaatttaaa cccgtattgt tttaaatctt tagatagttg
     5641 tgttgcagta tcatcaactg tgatacgatc agaagcatgt tcatactgca aatctttagg
     5701 cttaccattt acatatgacc ataaaaattt actaaaacta ccatatgctt gttcaatttt
     5761 taaataccct tgagcttgat taacaattgc ttctaatttt ttacgatgat gaacgatatt
     5821 tggaaaagtc attaagcggt cgatatcttg agcggtcatt tgcgctacct tttctggttc
     5881 gaaatcataa aatgcttctt cataggcttc tttctttttt aaaatagtta accaagatag
     5941 cccagcatgt tgtgattcta atgctaaaag tttaaacaat gctttgctat catagagcgg
     6001 ttgtccccat acataatcat gatagtctaa gtagactgga tctttagttc caaatgcgca
     6061 atcattcatt ttattcccac ctatacattg acttattttg tcaatcatta tactataagt
     6121 taagagttta atatatgatt tgggaagagt aagttcaaat tattttaaag agagcatatg
     6181 atttggtgtg agtatgtaaa tgatttttac tgaaggtagc ccacagagta tcttagctga
     6241 acgtgattaa tagtaggcta agacgtgttt gagcgttaat cataatctag agtgcttgaa
     6301 gagatgaagc atttcaaatt tcacgatgat agtattggta tgattattcc aattatgtta
     6361 aaagagtgaa tttgatatta atcatccttc ttgaatatag gtggtaccac ggaatatccg
     6421 tcctatttgt atataggatg gatatttttt atttttttag gaggtatagc aaatggaaat
     6481 gaaaccaaaa tatgatcctc gtgaagttga agcgggacgt tatgaagaat gggtaaagaa
     6541 tggttatttt aaaccgtcag aagataaatc aaaagaaaca tatacaatcg ttatcccgcc
     6601 accaaatgta actggtaaat tacatttagg acatgcatgg gatacaactt tacaagatat
     6661 cattacacgt atgaaacgta tgcaagggta cgatacgtta tacttaccag gtatggatca
     6721 tgctggtatt gcgacacagg caaaggtaga agctaaatta aatgaacaag gaataactag
     6781 atatgatctt ggtcgtgaaa agtttttaga acaggcatgg gattggaaag aagagtatgc
     6841 gtcatttatt cgtgcgcaat gggctaaatt aggtctaggt ttagattata gtagagaacg
     6901 ttttacttta gatgaaggtt taagtaaagc agttaaaaaa gtttttgttg atttatacaa
     6961 taaaggaatt atttatcgtg gcgaacgtat tataaattgg gatcctaaag cacgtacagc
     7021 tttatctgat attgaagtaa tacatgaaga tgttcaaggt gcgttttatc attttaaata
     7081 tccttacgct gatggtgaag gttttattga aattgcaaca acaagaccag aaacgatgtt
     7141 aggtgataca gcgattgttg ttaaccctaa tgacgaacga tacaaagatg taatcggtaa
     7201 aactgttata ttaccaatcg taggacgcga actgcctatt ttagcagatg agtatgttga
     7261 tatagacttc ggttctggtg ctatgaaagt gacaccagca catgacccta atgattttga
     7321 aattggtcaa agacatcaat tagaaaatat tatcgttatg gatgaaaatg gtaaaatgaa
     7381 cgacaaagct ggtaaatacg aaggtatgga ccgttttgat tgtcgtaaac agctagttga
     7441 agatttaaaa gaacaagatt tagttatcaa gattgaagat catgttcatt ctgtaggtca
     7501 ttcagaacga tctggcgctg ttgttgaacc atatttatca acacaatggt ttgtgcgcat
     7561 ggaagactta gcgaaacgtt cattagataa ccaaaaaaca gatgatcgta ttgattttta
     7621 tccgcaacgt ttcgaacata catttaacca atggatggaa aatattagag actggacgat
     7681 ttcaagacaa ttatggtggg gtcatcaaat tccggcttgg tatcataaag aaacaggcga
     7741 aatatatgtt ggagaagaag cgccaactga tattgaaaat tggcaacaag atgaagatgt
     7801 attagatacg tggttctcaa gtgctttatg gcctttctct acgttaggtt ggcctgattt
     7861 agaaagtgaa gactttaaac gatactaccc aacaaatgcc ttagttacag gttacgatat
     7921 tatcttcttc tgggtagcac gcatgatatt ccaaggctta gaatttacag atcgtcgtcc
     7981 atttaatgat gtattattac acggtttagt tcgtgctgaa gacgggcgta agatgagtaa
     8041 atcattaggt aatggtgtgg atccaatgga tgttattgac gaatacggtg ctgatagctt
     8101 gcgttacttc ttagcaacgg gttcatctcc aggacatgat ttaagatact caactgaaaa
     8161 agttgagtca gtgtggaact ttatcaataa aatctggaat ggggcacgtt tcagtttaat
     8221 gaatatcggt gaagacttta aagttgaaga tatcgattta agtggtaact tatcattagc
     8281 agataaatgg attctaacac gtttaaatga aacgattgca acagttactg atctaagtga
     8341 caaatatgaa ttcggcgaag ttggacgtgc attatataat ttcatttggg atgatttctg
     8401 tgattggtac attgaaatga gtaaaattcc aatgaatggt aatgatgaag aacaaaaaca
     8461 agttacacgt tcagtattga gttatacttt agacaatatt atgagaatgc tacatccatt
     8521 catgccattt gtaacagaga aaatatggca aagtttacca catgaaggtg acacaattgt
     8581 taaagcttca tggccagaag tgcgtgaatc attgattttt gaagaaagta aacaaacaat
     8641 gcaacaactt gttgaaatca ttaaatctgt aagacaatca cgtgtagaag taaatacgcc
     8701 attgtctaaa gaaataccta ttttaattca agctaaagat aaagaaattg aaacaacttt
     8761 atcacaaaac aaagattatt taatcaaatt ctgtaatcct agtaccttaa atattagcac
     8821 tgacgtggaa attcctgaga aagcaatgac atcagttgta attgcaggta aagtggtatt
     8881 accattagaa gggctaattg atatggataa ggaaatcagc cgtttggaaa aagaattagc
     8941 taaacttcaa agcgaattag atagagtaga taaaaagctc tctaatgaaa actttgtaag
     9001 taaagcacct gaaaaggtta taaatgaaga aaaacgtaaa aaacaagatt atcaagaaaa
     9061 atatgatggt gtgaaggcaa gaattgaaca attaaaagca taggagttag taacaatgaa
     9121 ttacctagag agcttgtatt ggatacacga aagaactaaa tttggcatca aaccaggtgt
     9181 taaacgtatg gaatggatgc tagcacaatt taataatcct caaaataaca ttaagggtat
     9241 tcatgtaggt ggcacaaatg gtaaaggatc tacagttgct taccttagaa cagctttagt
     9301 tgaaaatggt tatgaagtag gtacatttac gtcgccgttt attgaaacat ttaatgaacg
     9361 aattagtcta aatggtgtgc caatatcaaa tgacgctatt gtagaattag tatcacgtat
     9421 taaaccagta agtgaaatga tggaacgtga aacagattta ggtgttgcaa ctgaattcga
     9481 aataatcaca gcgatgatgt ttttatattt tggtgaaata catcctgttg attttgtcat
     9541 tgttgaggct ggattgggta taaagaacga ttcgacaaat gtctttacac cggttttatc
     9601 aatcttaact agtatcggtc tagaccatac agatatttta ggtggtacgt atctagatat
     9661 tgctagggat aaaggcgcga ttataaagcc taacgttcca gtgatatatg ctgttaaaaa
     9721 tgaagatgca ttaaaatatg ttcgtgaacg cgcaattgaa caacatgcaa agccaattga
     9781 attagataga gaaattgttg ttgtatcgca aaatgatgaa tttacttacc gttataaaga
     9841 ttatgaatta gaaacaatca ttttaagtat gttaggtgaa catcagaaac aaaatgctgc
     9901 attagccata acagctctta ttgaattaaa tgaacaagga ttaattgaat tagatttcaa
     9961 taagatgata gacggtattg aatcagttcg ttggactgga cgtattgagc aggtgcatga
    10021 caaaccttta atcattttgg atggcgcaca taattcagag agtatagatg ctctaattga
    10081 tacaattaaa cagtaccatg ataaagaaaa agtagatatt ttgttctcag caataaacgg
    10141 aaaaccgatt aacgagatgg tcaaacattt aagtttaatt gcgcatacgt tttatgcaac
    10201 tgaatttgat tttccgaaag cgttacgcaa agaagaaatt gtaggtagta ttgaaaatga
    10261 tgaaatacaa ttagtagatg actacgttga atttataaaa aattatcaag gtgatacatt
    10321 agtaattacc ggtagtctgt atttcataag tgaagttaaa tcaaaattag actttgaagc
    10381 attataaaac taaaactaaa atagccatga agaatctatt taatcaatag gtagagcttc
    10441 atggttattt tttatgctct aaatcgtatg tgtttataag gttcaatttc ttgccattat
    10501 taattttgcc gagcagtaat gggctgttaa agtatccatc aattatgctt agcaatattg
    10561 aaaaatttaa atgaaaaagc gattcaattc ttgaaaaata gtgtatattt acattttgat
    10621 aataactaac tataatttac ataaggaggt tgtcatttgg tagtattgtt atcttatagt
    10681 tgcagttgta tctttagttt tctatatcaa tttatatcta tagaagaaac gtcatttgat
    10741 tatttacata gaagatcgaa atgtgattat tgtaattcat cactcaaatg gtatgaatta
    10801 atgccgatta ttagtttttt attattaaaa gggcgatgtc gaaactgtcg aaagcgtatt
    10861 tccctaacac atttcttagg ggaaaccttt gctataatac ctatcgtctt tattaagtat
    10921 gatttcacat acgtaaatgc tacgctattt ataactacat atgtttttct gcttattttt
    10981 actatgaccg atatcacttc tttaatgtta gattgtcgct taattataat ttattgtatc
    11041 gtttctctct cgttaagtat gatttatcca gtagctttta tcattattag tatgaccacg
    11101 catatattct actttttatt tcgggcatat attggttatg gtgacgtttt actaatatct
    11161 gcactttctt tgtttttccc tctccaattc actatttatg tcattttatt tacatttgtc
    11221 attgctggtt tagttgcttt aattaccatg atatttaagc cgattaaact attacccctt
    11281 gttccattta tatttatttc attttttatc aattcacttt tttataatga tatccatcaa
    11341 tttttaggag gcgtatattt ttgaaaatta aagaaatggt aacttcagaa atgccaagag
    11401 aacgtttgtt aagccatggt gcaaaaagtc tttcgaatac agaattatta gctatattaa
    11461 ttaacaccgg aagaaaagga ttctcgagca tagacattag taatgaactg cttaaatctg
    11521 cttcgaatct gaatgaattg aaaaaatctt caattaacga tttgatacaa gttaaaggga
    11581 ttggattaca aaaagcaatt actttaaaag cagcatttga gctaggggaa aggatgggga
    11641 gaagagctga aaataatcgt ataaaaataa cgcaaccaag tgatgttgct gattatatga
    11701 ttccaacaat gaaagattta acacaagaac attttgtcat tttattgtta aattcaaaaa
    11761 atgtagtgat taaagaaacc tgtgttttta aaggtacatt aaatagttcg attgtacatc
    11821 cacgtgaaat ttttagtatt gcggtgagag aaaatgccaa tgcaatcatc gcagttcata
    11881 atcatccatc cggtgatgta acgccctcac aagaagatat cataacaaca atgaggttga
    11941 aggagtgtgg tttgatttta gggatagatt tattggatca tattataatc ggtgataata
    12001 gatttacgag tcttgtagaa gcgggttact ttgatgaaaa tgattgaagt tgatagaatt
    12061 aatgacgtat cttgtgtata atacctacga agtactttca ttggaggaaa aatagtgact
    12121 ctatttatta ttatcggggt tctcgtgcca atggtttata ccatgcagtt aaatattaaa
    12181 aatgaacctg taacaaagcg caatctatta ataacattag ctttatctac gttaggtatt
    12241 ttagtaacgg cattagcagg tgtgattgtt acaaaacaag cttttccttt attaagtgta
    12301 gcaataggct caatttttac aggaatcgtt tggggccttt tactaagcgg tagctatgcg
    12361 ctgatacgat ttttatctaa cgcatttggg cgtaagtaat agtaatctgt tcatcaagta
    12421 gtatacgtgc ttgaaaacaa attaaaactc ctaatgtgga actagttaaa aaattctaaa
    12481 ctagtacatt aggagttttg ttatgcagaa taaatttaat tgttaaattg aaagtgtggt
    12541 aaaaacccac tatttatttg aaaaaaatcg agcaaccata ttaaaccttg atatcctaag
    12601 taaatacata ataaaacaag tccgacataa attaaaaatc gcaaaataga aagtccaact
    12661 ctaaaaagga ggatgactaa tagtgctatt aaaataattg ttagtatact caatgctaca
    12721 aacctcctca tatgctttta aatcataaca aaattataag ctattttaaa tgaacgtgca
    12781 tcagtcctaa gatatatcat gattaaggct gaagactgag gaaaatatac ataataaaat
    12841 atataattag atgaagacat tggaggcgaa ctatgagatt gatatttagt atgattaaaa
    12901 acattatagc agtaatagca attgtgataa ttgtttatat cgccttgaaa tacgcacctt
    12961 ttttaaaaga acaagattgg aatcctgtaa atcacgataa tgatcaaatg aatcaagtta
    13021 cgcaacccac taatgatgcc caacatgttt atgtaagtgg tgagaaatat tcattaaaag
    13081 aaaatgattt aataaagaat gtaccattaa gccaaattaa aaatgtattt aaaatgatag
    13141 ataaacaaga gtttatggct gtgtctggaa tgaatcgcat ggcttataat gatcaatata
    13201 ttataggtca aagaggagac gaatttattc tttataaatt tggagatgag tcaatgcgtg
    13261 tttacaatac tgaatttgaa atgcaacaag acttaaatga attagggcaa aatttacaac
    13321 taaaacccga aaatgcttat caataggaaa ttattgcata agaaatatat tttagttatc
    13381 ctgtcactag aataaattta aaaggttggt gtagttcaaa tggcagaaca atcaaaacag
    13441 aaacaagcta atgaacaaca aaaggcccag aacttattcg cacgttggag acaacttcag
    13501 aattcgaaca gtgaatcctc gaacgataca aacaaataat tttaaaattt aaatcgatta
    13561 tttaaactac tagtgactgg aatgtttgct agtagttttt ttgcttttaa aatgccacaa
    13621 attttttacg gagttgttca ttttcaaact aattcgtagt tagtgcttta tttcataatg
    13681 ggagtaatat aaaatagaaa aggatataca atattttgac ataatagagg tgttctgggt
    13741 gcttaagttt tttaaaaata acaaattaat tgttgtttta tgtgcaatta tcgtttttat
    13801 tgcattaatt gggctgtcca tacgttcaca atctcaatca cctcctgaac aatatatagg
    13861 tgattctgtg tcttttggac aacgagttgt gagttatcca gttaattttg ttgctggtac
    13921 gattggggac ttttttaaaa aaggagattc taaagaatct aaaaataaga ttagccagtt
    13981 agaatctaag aaccaacaat tagaagcgga aaatgaaaaa ttaaaaaaag agcttgattt
    14041 aaaagatatt tcaaaatttg atcctatttc tactacggtt ttagcaagaa atccggatca
    14101 gtggatgaat acaattgtta ttgataaggg atctaaatca ggtataactt caaatatggc
    14161 tgtgatgaca tcaaaaggtt ttgttggaag agttactaaa gttaataaat tttcttcaca
    14221 agttgattta atctcaacta atacacgtgc gggtaaatta tctgtaaata tacaacacgg
    14281 ctctaaaaat atatttggct taattgatcg ttatgatgaa aagaactcag aacttgtaat
    14341 tagtgatatt aataatagag ataatatctc aaaaggtgat aaagtcgtta caagtggatt
    14401 agctgatcaa ttaccaagtg atttatatat aggagaagtg actaaggttc aaaatgatca
    14461 atatggctta gctaaagaag ttaggattaa gactggtgca gacttaacag atttgagtca
    14521 tgtttatgtt gcaaaaagag atcctaaaac aattcctgat gatgaaagca gggataaata
    14581 atgcgtacac tgtattattt tttgataggt attttactat tttatataga tactgcaatt
    14641 gggcttctta ttccaatgaa cataggtaag tttgaacttg tatttgtacc acaccttaca
    14701 tttatgtata ttttaatgat ggtagtgtat cgtggctttg gcgtatcatt attgctcagc
    14761 atatttttag gcgtaatgac tgatgtatac tttggtagta tttatggagt gtacttattt
    14821 ggctatatat tgtttttagc acttatagat cgattcttta aaatttttta caaagatcat
    14881 tcgatgttat tcattattat attagccagc accttattat tagaagtcta tgtggcatta
    14941 atatacggta tgttaggatt cattcaattt gatattattc attttgtagt ctttagatta
    15001 ttgccaacat taattatgaa ttttgtactg ttgattatgc tttatccgtt gattataaag
    15061 ttccttaaaa aaacaaacaa tgacattgac atgaaacgtc gtcaatggta agatgcaata
    15121 gttgagtagt tataagtagc tacatacaac cgctcaaata taggttcaag aacattttta
    15181 atgaagaaag cgattttcaa tcaaggcgtt gaaggaagaa acgagaagag tttatcttta
    15241 taaatgactc gagttacgac cgaaacaacg tagagtgcaa acgcttgact caataacgaa
    15301 atgtcaccta tgtatggcgt gacttattat acaggaggtg caaagtatgt ttgctattat
    15361 tgaaacaggt ggaaaacaaa tcaaagtaga agaaggtcaa gaaatcttcg ttgaaaaatt
    15421 agacgtaaac gaaggagata cttttacatt tgataaagta ttatttgtag gtggagattc
    15481 agttaaagtt ggagcgccaa cagttgaagg tgcaacagtt actgctactg ttaataaaca
    15541 aggtcgcggt aaaaaaatta ctgtattcac atacaaacgt cgtaaaaatt caaaacgtaa
    15601 aaaaggccat cgtcaaccat acactaaatt aacaatcgat aaaatcaacg cgtaattatt
    15661 atgattactg ttgatattac agttaatgat gaaggcaaag taacagacgt tattatggat
    15721 ggccatgctg accatggtga atatggtcat gatatcgttt gtgctggagc ttcagctgta
    15781 ttgtttggta gtgttaatgc gattatagga ttgacatctg agagaccaga tatcaattat
    15841 gacgacaatg gtggtcattt tcatataaga agcgttgata caaataacga tgaagcgcaa
    15901 ctaattcttc aaacaatgct tgtgtcttta caaactattg aagaagaata taatgagaat
    15961 attagattaa attataagtg aggtgcattc cgatgttaaa attaaactta caattcttcg
    16021 catctaaaaa aggggtaagt tctacaaaaa acggacgtga ctctgaatca aaacgcttag
    16081 gtgctaaacg tgctgacggt caattcgtaa caggtggttc aattttatat cgccaacgtg
    16141 gtactaaaat ttaccctggt gaaaatgtag gtcgtggtgg cgatgataca ttattcgcta
    16201 aaatcgacgg cgttgttaaa ttcgaacgta aaggtcgcga caaaaaacaa gtttctgtat
    16261 atgcagtagc tgaataattt tgtctagtta acaccagaag tgaatcttct ggtgtttttt
    16321 actttttata aaacgttttt cattattatt ttcattttgt tattaaaaaa tgggaatttt
    16381 agacgttata ctaaatgtgc gactgcataa gagcccctaa tcgctaaact ataaggggct
    16441 ctaatatgct ataattattg ggaaaatgaa aattatatgt aaaagaggtg agatatatgt
    16501 ttgtcgatca agtcaaaata tctcttaaag ccggtgatgg tggtaatggt attaccgcat
    16561 acagaagaga aaaatatgta ccatttggtg gaccagctgg cggtgacggt ggtaaaggtg
    16621 cttcagtcgt atttgaagtg gatgaaggtt taagaacgtt attagatttt agatatcaac
    16681 gtcattttaa agcaagcaaa ggtgaaaatg gccaaagtag taatatgcat ggtaaaaatg
    16741 cggaagattt agtattaaaa gttccacctg gtacaattat taaaaatgtt gaaacagacg
    16801 aagtgttagc agatcttgtt gaagatggtc aaagagctgt agtagcgaag ggcggtcgag
    16861 gtggccgagg taattcacgt tttgcaacac ctagaaaccc tgcacctgac ttcagtgaaa
    16921 aaggtgaacc aggcgaggaa ttagatgtat ctttagaatt gaaattatta gctgatgtag
    16981 gattagtagg tttccctagt gtgggtaaat cgactttatt atctatcgtt tcaaaagcta
    17041 agcctaaaat tggggcatat cattttacaa cgattaaacc aaatctaggt gttgtttcaa
    17101 cacctgatca acgtagtttt gttatggcag atttaccagg tttaattgaa ggtgcatctg
    17161 atggcgttgg attaggacat caatttttaa gacatgtaga gagaacaaaa gttattgttc
    17221 acatgattga tatgagcggt tctgaaggta gagaacctat tgaagattat aaagtcatta
    17281 atcaagaatt agctgcgtac gagcaacgtt tagaagatag acctcaaatc gtagtagcta
    17341 acaagatgga tttacctgaa tcacaagata atttaaactt gtttaaagaa gaaattggcg
    17401 aagatgtgcc agttattcca gtttcaacaa taacgcgtga taatattgat caattattat
    17461 atgcaatagc agataaatta gaagaatata aagatgttga cttcacagtt gaagaagagg
    17521 agtcagttgg cattaaccga gtattatata aacatacacc gtcacaagat aaatttacaa
    17581 tttcaagaga tgatgatggt gcttatgtgg taagtggtaa tgctattgaa agaatgttta
    17641 aaatgactga ctttaacagt gatccagcag tacgtcgatt tgctcgtcaa atgcgttcga
    17701 tgggtattga tgatgcactt agagaacgtg gttgtaaaaa tggtgatatc gttagaattc
    17761 ttggcggaga atttgaattc gttgaatagg agcgtaacat gatggacaat aaagattata
    17821 aaaagtttta tttaattaga gaagatgtct tgcctgaatc cgtggttaaa acattgaaga
    17881 ttaaagatgc cttaaaaagt gatccgacat tgtccattta tgatgccgtt aaacagtttg
    17941 atctatctag aagtgctttt tataaatata gagaaacgat atttccagta gacgataaaa
    18001 tgcttgacca tcgagaattt acattaattt tatatgtaac agatattgtg ggtatgttgg
    18061 cacgtgtact agatgttata tcaaagttag aactatctgt attaacgatt catcaaagta
    18121 ttccaatgga agaaaaagca acaataacat tatcactgaa tgctaaatct aaagaaactg
    18181 cagtagaaga tgttattggc gctttgagaa atttagatta tgtatcaaaa gtagaattaa
    18241 ttagtatgag tatgtaagga tgtgcctata atgtacgcgt atgtcaaagg taagttaaca
    18301 catttatatc ctacacacgt agttgttgaa actgctggtg ttggttatga aattcaaaca
    18361 ccaaattctt atcgttttca aaagcaacta gatcatgaag ttttaattca tacatcttta
    18421 attgttcgtg aagatgcaca attattgtat ggatttagta gtgaagaaga gaaagatatg
    18481 ttcttgagtt taattaaagt tactggtatt ggtccgaaat cagctttagc tattttagcg
    18541 acaagtacgc ctaatgaagt aaaacgtgcc attgaaaatg aaaatgatac gtatttaact
    18601 aaattcccag gaattggtaa gaaaacggca agacagattg tcttagattt aaaaggtaaa
    18661 gtgaaaatca ctgaagaaga tagcgattca ttattacaag tagacgctac ttcgacggtg
    18721 caagatcaat tcgtgcaaga agcaatgtta gcgttagaag cattaggtta ttctaaacga
    18781 gagcttgcaa aagttgagaa aacgttaaat aaaaataaat atgactcagt tgatgaagct
    18841 gttaaggcag gtcttcaatt agttgtatct taattttaaa tagattaata ggggaagtgt
    18901 tgtcatgaat gagcgtatgg ttgatcaatc aatgcatagt gaagaaactg atttcgaatt
    18961 gtcgcttaga cctacgagat tacgacaata tattggtcaa aattcaataa aaagtaattt
    19021 agaagtattt attaaagcgg ctaaacttcg tcatgaacca ttagatcatg tattgctttt
    19081 tggcccccct ggattaggta agacaacatt atctaatatc attgccaatg aaatggaagt
    19141 taatatacgt acagtatcag ggccttcatt agaaagacct ggtgatttgg ctgcaatttt
    19201 atcaggactt caacctggag atgttttgtt tattgatgaa atacacagac tgagtagtgt
    19261 tgttgaagaa gtgttatacc ctgcaatgga agatttcttt ttagatatta tcattggtaa
    19321 aggcgatgag gctagaagta tccgtatcga cttacctcca ttcactttgg taggtgcaac
    19381 aacgcgagct ggcagcttaa caggtccact aagggatcga tttggtgtgc acttaagatt
    19441 agaatattat aatgaatcag atttaaaaga aatcattatt agaacagctg aggttttagg
    19501 cacaggtatt gatgaagaaa gtgccattga acttgctaaa cgttctagag ggactccaag
    19561 agtagcaaat cgactattga agcgggtaag agacttccag caagtgaatg aagatgaaca
    19621 aatatacatt gaaacaacga agcacgcatt aggtttactt caagttgatc aacacggact
    19681 agattacatt gatcataaaa tgatgaactg tattattaag cagtataatg gtggacctgt
    19741 tggtttagat acgattgccg taacaattgg tgaagaacgt attacaattg aggacgttta
    19801 tgagccattt cttattcaga aaggcttttt agaacgtacg ccacgtggca gaaaagcaac
    19861 accattagct tatgaacatt ttgcaaagtc gaatgaggag agagaataac gtgaattttg
    19921 aagaatttga ctatgactta ccagaatcat taattgctca aacgccttta aaagatcgtg
    19981 atcatagtcg tttattagtc atggatagag aaactggtga aatgaaacat ttacatttca
    20041 aagatatcat tgagtatttt agacctggtg atacattagt gcttaacgat acgcgagtaa
    20101 tgccagctag actttttggt ttaaaagaag aaactggtgc aaaagttgaa atgttaatgt
    20161 taactcaaat tgaaggtaat gattgggaag tcttactgaa accagctaag cgtattaaag
    20221 ttggtaataa attgaatttt ggtaatggca aaattatagc tgaatgcata aaagaaatgg
    20281 atcaaggtgg acgcatcatg cgtttacatt atgaaggtat tttacaagaa agattagatg
    20341 aattagggga aatgccactg ccaccataca tcaaagaacg tttagatgat ccagatcgtt
    20401 atcaaacagt ttacgctaaa gaaagtggtt cagcggcagc accaacagca ggattacatt
    20461 ttactgatga gttattaact gaaattaaaa ataaaggtgt taatatcgca tttgttacat
    20521 tacatgttgg gttaggtacg tttagaccgg tgagcgtcga tgatgtgaat gaccacgaaa
    20581 tgcatagtga atattatcaa atgacacaag aaacagctga tttattaaat gatactgagt
    20641 ccaaaggaca tcgcattata tcagttggta caacttcaac acgtacactt gaaacaattc
    20701 gacgcgatca tgataaattt gttgaaacga gtggctggac taatatattt atttatccag
    20761 gatttgattt taaagcaatt gatggccaga ttactaattt tcatttacca aaatcaacat
    20821 tagttatgct agtatcagcg tttagtactc gtgaaaatgt tctgaatgct tataaaacgg
    20881 cagtaaattt agaatataga ttctttagtt ttggcgatgc aatgttaatt atataaaaag
    20941 aatgtgagga ttttgaatat gcctgcagta acatacgaac acattaaaac ttgtaaacaa
    21001 tcaggtgcgc gtttaggtat cgtgcacaca ccacacggtt catttgaaac acctatgttt
    21061 atgccagttg gtactaaagc aaccgttaaa acaatgagtc cagaagagtt aagacaaatt
    21121 gaagcaaaaa tcattttggg caacacatat catttgtggt tacaacccgg aaatgatatt
    21181 atcaaacacg ctgggggatt acataaattc atgaattggg atggtccgat tctaacagat
    21241 tcaggcggtt tccaagtgtt tagtttaagt aatttacgta aaattacaga agaaggcgtg
    21301 gaatttagac atcatactaa tgggtctaaa ttatttttga gtcctgagaa atcaatgcaa
    21361 attcaaaatg atttaggttc tgatattatg atggcatttg atgaatgtcc accaatgcct
    21421 gctgaatatg attatgtaaa aaaatctatt gaacgtacaa cacgttgggc gaaaagatgt
    21481 ctagatgcac accaaagacc tgaagatcaa gcattgttcg gcattataca aggtggcgaa
    21541 tatgaagatt taagagaaca aagtgcaaag gatttagtag aattagattt tcctggttat
    21601 gcaatcggcg gtttgtcagt tggtgaacct aaaccggtta tgtataaaat ggttgaacat
    21661 acagagcagt ttatgcctaa agataaacca agatatttaa tgggtgtagg atctccagat
    21721 gcgttaatcg aatgtagtat tcgcggcatg gatatgtttg attgtgtctt accgacacgt
    21781 attgccagaa atggtacttg tatgacatcg caaggtcgtt tagttattaa aaatgcaaaa
    21841 tttgcagatg atttaagacc gttagatgag aattgtgact gttatacatg tcaaaactat
    21901 tcaagagcgt atatacgtca tttaatcaag gcagaggaaa cttttggtat tcgtcttact
    21961 actattcata atttacattt tctgctaaaa ttaatggaag atataagaca agccattcga
    22021 gaagatcgtc ttttagattt caaagaagaa ttcttcgagc aatatggatt aaatgttgag
    22081 aacccaaaaa acttttaagc aagaggagcg tataaaatgc aattttcatt actaatatat
    22141 atagtcgtaa tttttgcggt tatgtatttc ttgatgatta gaccacaaca aaaacgtgcg
    22201 aaacagcatc gtgagttgat taataacatt caatctggtc aaagaattac aactattggt
    22261 ggtattaaag gtactgttaa agcagtagat gaaacaactg ttgttattac agttaatggt
    22321 catggtactg aattaacttt cgaaaaacct gctattaaac aagttgaccc ttcataacga
    22381 gaagtggtat taaaaaggat gaaacgaata gcgtttcatc ctttttaagt tataagtagt
    22441 atgtagaata gcttattttt attccaaaaa aagaaattgt gatacataat aattgttaaa
    22501 tttaaattaa atatatctat gactattttt actccctgct ctagtatgtt aatatgtata
    22561 aggctaataa attttgtaag aaagatttaa cttgcgttat aaaatagtat tcaagtattc
    22621 taaatacata cgtaaataat gaggtgttca tgtgaagaaa agtagtagaa taattgcgtt
    22681 cttgctttta gtcgtattgt tgtttgccgg aatggctgca acttataaaa gtgttataaa
    22741 gaatgtcaat ttaggacttg atttgcaagg tggatttgag gtgctttatc aagtcgatcc
    22801 tttaaataaa ggcgacaaaa tagataaaaa agccttacaa tcaacagcgc aaacattaga
    22861 aaatcgtgtt aacgttttag gtgtttccga acctaaaatt caagttgaag aacctaatag
    22921 aattagagtt caacttgcta gtgtaacaga ccagaatgaa gcacgtaaaa ttttatcttc
    22981 tcaggctaat ttaacaattc gcgatgcaga agataaagta aaattaagcg gttctgatat
    23041 taaacaggga tctgccaaac aagaatttaa acaggaaaca aaccagccta cagttacttt
    23101 taaagtaaaa gacaaaaaca aatttaaaaa agtaacggaa gaaatctcta agaaacgtga
    23161 taatgtcatg gttgtttggt tagatttcaa aaaaggtgac agctataaaa aagaagcaca
    23221 aaagaaaaac cctaaattta tttctgcagc aagtgttgat caaccaatca attctgatag
    23281 cgtagaaatc tctggtggat ttaaaggaca agaaggcgtt aaaaaagcaa aacaaatcgc
    23341 cgaattgtta aatgcaggtt ctttaccagt tgacttgaaa gaaatttatt caaactcagt
    23401 aggtgcacaa tttggtcaag atgcattaga taaaactgtg tttgcatcgt ttattggtgt
    23461 agcattaatt tatttattca tgcttggatt ctaccgatta cctggtttag tagcgattat
    23521 tgcattgaca acttatatct atctaacgtt agtagcattt aatttcattt ccggggttct
    23581 aactttacca ggattagcgg cgttggtatt aggtgtaggt atggctgtag atgccaatat
    23641 tatcatgtat gagcgtatta aggatgaact tcgaataggt agaacgataa agcaagcctt
    23701 ttctaaagca aacaaaagtt cgttcctaac aatttttgat tctaacttaa caacagttat
    23761 cgccgcagca gtattattct tcttcggtga aagttcagtt aaaggtttcg cgacaatgtt
    23821 attattaggt attctaatga tctttgttac agccgtgttc ttatcaagat tcttattatc
    23881 attacttgtt tcatcaaata tattcaa
//
LOCUS       DAHHOJ010000032        23797 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.032, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000032 DAHHOJ010000000
VERSION     DAHHOJ010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23797
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.032"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            149..1462
                     /gene="murT"
                     /locus_tag="NBH32_001091"
     CDS             149..1462
                     /gene="murT"
                     /locus_tag="NBH32_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II isoglutaminyl synthase subunit MurT"
                     /protein_id="HCD1514709.1"
                     /translation="MRQWTAIHLAKLARKASRAVGKRGTDLPGQIARKVDTDILRKLA
                     EQVDDIVFISGTNGKTTTSNLIGHTLKANNIQIIHNNEGANMAAGITSAFIMQSTPKT
                     KIAVIEIDEGSIPRVLKEVTPSMMVFTNFFRDQMDRFGEIDIMVNNIAETISNKGIKL
                     LLNADDPFVSRLKIASDTIVYYGMKAHAHEFEQSTMNESRYCPNCGRLLQYDYIHYNQ
                     IGHYHCQCGFKREQAKYEISSFDVAPFLHLNINDEKYDMKIAGDFNAYNALAAYSVLR
                     ELGLNEQTIKNGFETYTSDNGRMQYFKKERKEAMINLAKNPAGMNASLSVGEQLEGEK
                     VYVISLNDNAADGRDTSWIYDADFEKLSKQQIEAIIVTGTRAEELQLRLKLAEVEVPI
                     IVERDIYKATAKTMDYKGFTVAIPNYTSLAPMLEQLNRSFEGGQS"
     gene            1464..2195
                     /locus_tag="NBH32_001092"
     CDS             1464..2195
                     /locus_tag="NBH32_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1514710.1"
                     /translation="MHELTIYHFMSDKLNLYSDIGNIIALRQRAKKRNIKVNVVEINE
                     TEGITFDECDIFFIGGGSDREQALATKELSKIKTPLKEAIEDGMPGLTICGGYQFLGK
                     KYITPDGTELEGLGILDFYTESKTNRLTGDIVIESDTFGTIVGFENHGGRTYHDFGTL
                     GHVTFGYGNNDEDKKEGIHYKNLLGTYLHGPILPKNYEITDYLLEKACERKGIPFEPK
                     EIDNEAEIQAKQVLIDRANRQKKSR"
     gene            2281..2391
                     /locus_tag="NBH32_001093"
     CDS             2281..2391
                     /locus_tag="NBH32_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514711.1"
                     /translation="MDGNNQIDVCFFVLVNNYYTMSIYPRIINSNGDYAT"
     gene            2420..2833
                     /locus_tag="NBH32_001094"
     CDS             2420..2833
                     /locus_tag="NBH32_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514712.1"
                     /translation="MKPTKVILRDASYLHSKTSITFILKDVVIEEDNKIYYFDTSATF
                     EDQEVKFEFALFDSDMDNLKHMDYDNPVTEIYFIEPDLHFTIIDFNQELLCIYIDFDS
                     GLRHSNMATESGISLRINVAKSDFTKFINELASLH"
     gene            complement(3264..4250)
                     /locus_tag="NBH32_001095"
     CDS             complement(3264..4250)
                     /locus_tag="NBH32_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1514713.1"
                     /translation="MNDQWYKHLIGARTIKTGIAIFLTAVFCMALDLTPIYAILTAVV
                     TIEPTAKASLIKGYRRLPATVIGAGFAVLFTYLFGDQSPFTYALSATFTILFCTKLKL
                     QVGTNVAVLTSLAMIPGIHDAYIFNFLSRTLTAIIGLVTSGLINFMVFPPKYYGQVEE
                     KLSKTDALMYKLFYNRCQELILSRLQSDKSEKAYKNIFNLNNQVETLISYQRDELSYH
                     KKKECDWKLLNQLTKRAYTNRLFITHLSNIIYLPKNTRVNFSGDEKMALLKISSSIKD
                     IFYDGSFKREDDSVETLRSTIKALEISGENQIKSHILYEVLMIYRLLDSRYA"
     gene            4779..5537
                     /gene="map"
                     /locus_tag="NBH32_001096"
     CDS             4779..5537
                     /gene="map"
                     /locus_tag="NBH32_001096"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCD1514714.1"
                     /translation="MIVKTEEELQALKEIGYICAKVRNTMQAATKPGITTKELDNIAK
                     ELFEEYGAISAPIHDENFPGQTCISVNEEVAHGIPSKRVIREGDLVNIDVSALKNGYY
                     ADTGISFVVGESDDPMKQKVCDVATMAFENAIAKVKPGTKLSNIGKAVHNTARQNDLK
                     VIKNLTGHGVGLSLHEAPAHVLNYFDPKDKTLLTEGMVLAIEPFISSNASFVTEGKNE
                     WAFETSDKSFVAQIEHTVIVTKDGPILTTKIEEE"
     gene            5754..6140
                     /locus_tag="NBH32_001097"
     CDS             5754..6140
                     /locus_tag="NBH32_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514715.1"
                     /translation="MNYVERYIEQFLRATVRNNIKHYLLMLDEKMKNLDDYMRYLITK
                     KEQLSKLIDSLMLTLENKYIDIAEAFQIQCAREINNQEIENIKSELNKVEAYYAQIET
                     QIQQTSTEKIATEKTSYLINYMNAVA"
     gene            6155..6856
                     /locus_tag="NBH32_001098"
     CDS             6155..6856
                     /locus_tag="NBH32_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HCD1514716.1"
                     /translation="MTHKYISTQMLIIFTALMIIANFYYIFFEKIGFLLVLLLGCVLV
                     YVGYLYFHKIRGLLAFWIGALLIAFTLLSNKYTIIILFVFLLLLIVRYLIHKFKPKKV
                     VATDEVMTSPSFIKQKWFGEQRTPVYVYKWEDVQIQHGIGDLHIDLTKAANIKENNTI
                     VVRHILGKVQVILPVNYNINLHVAAFYGSTYVNEKSYKVENNNIHIEEMMKPDNYTVN
                     IYVSTFIGDVEVIYR"
     gene            6853..7896
                     /locus_tag="NBH32_001099"
     CDS             6853..7896
                     /locus_tag="NBH32_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1514717.1"
                     /translation="MNHYIRTIGSMLILVYSMLAAFLFIDKVFVNIIYFQGMFYTQIF
                     GIPVFLFLNLIIILLCIIVGSVLAYKINQQNDWIKTQIERSMEGETVGINDQNIEIYS
                     ETLDLYHTLVPLNQELHKLRLKTQNLTNENYNINDVKVKKIIEDERQRLARELHDSVS
                     QQLFAASMMLSAIKETKLEPPLDQQIPILEKMVQDSQLEMRALLLHLRPLGLKDKSLG
                     EGIKDLVIDLQKKVPMKVVHEIQDFKVPKGIEDHLFRITQEAISNTLRHSNGTKVTVE
                     LFNKDDYLLLRIQDNGKGFNVDEKLEQSYGLKNMRERALEIGATFHIVSLPDSGTRIE
                     VKAPLNKEDSYDD"
     gene            7886..8515
                     /gene="vraR"
                     /locus_tag="NBH32_001100"
     CDS             7886..8515
                     /gene="vraR"
                     /locus_tag="NBH32_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator VraR"
                     /protein_id="HCD1514718.1"
                     /translation="MTIKVLFVDDHEMVRIGISSYLSTQSDIEVVGEGASGKEAIAKA
                     HELKPDLILMDLLMEDMDGVEATTQIKKDLPQIKVLMLTSFIEDKEVYRALDAGVDSY
                     ILKTTSAKDIADAVRKTSRGESVFEPEVLVKMRNRMKKRAELYEMLTEREMEILLLIA
                     KGYSNQEIASASHITIKTVKTHVSNILSKLEVQDRTQAVIYAFQHNLIQ"
     gene            complement(8628..9845)
                     /locus_tag="NBH32_001101"
     CDS             complement(8628..9845)
                     /locus_tag="NBH32_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HCD1514719.1"
                     /translation="MSKKDHSSSKYLNSVKEAQEESKKKNKSNPKIDVDRTYIEPQQF
                     QSKKPKKDDQVFFLSRLNKPAKYKKDSNFLSYLIYRIGKDDASGLAAQMTYHFVLAMF
                     PMLLFLLTLLPFFNIKQSQITNMLSNAPAETSTLIKSVIGDITQNSSGGLLSIGLILA
                     IWSASNGMTAIMNSFNVAYDVEDSRNGIVLKLLSVVFTVVMGVVFVVALALPTLGSVI
                     SHFLFGPLGFDEQVKWIFNLIRIVLPIIIIFIIFIVLYSVAPNVKTKLKSVLPGAVFT
                     SIIWLAGSFGFGWYISNFGNYSKTYGSIAGIIILLLWLYITSFIIIVGAEINAIIHQR
                     SVIKGKTPEEAALEHDDNNKNHYNENTRYEYDEDSNATHQRTYHVDEHPSDTYPEDDK
                     NIKDKIVDKLKKD"
     gene            complement(10123..10398)
                     /locus_tag="NBH32_001102"
     CDS             complement(10123..10398)
                     /locus_tag="NBH32_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1514720.1"
                     /translation="MENKFVPGILIGAVIGGAISLADKSTRQALVQSVKDAKNGNRTR
                     KPSKVSKIKDEVLYWKDVVEEIRRNNPELERSLKDAKETFVNRKNQR"
     gene            complement(10405..10869)
                     /locus_tag="NBH32_001103"
     CDS             complement(10405..10869)
                     /locus_tag="NBH32_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HCD1514721.1"
                     /translation="MVDVAFVCLGNICRSPMAEAIMRQRLKDRNIHDIKVHSRGTGSW
                     NLGEPPHEGTQKILNKHNIPFDGMISELFEATDDFDYIVAMDQSNVDNIKSINPNLKG
                     QLFKLLEFSNMEESDVPDPYYTNNFEGVYDMVLSSCDNLIDYIVKDANLKEG"
     gene            11016..11216
                     /locus_tag="NBH32_001104"
     CDS             11016..11216
                     /locus_tag="NBH32_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1128 family protein"
                     /protein_id="HCD1514722.1"
                     /translation="MTNEEKVLAIREKLNIVNQGLLDPEKYKNANEEELTDIYDFVQS
                     REKLSPSEVTAIADALGQLRHD"
     gene            11219..12475
                     /locus_tag="NBH32_001105"
     CDS             11219..12475
                     /locus_tag="NBH32_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCD1514723.1"
                     /translation="MGILTNYKEKLQQYAELLVKVGMNVQPKQPVFIRSSVETLELTH
                     LIVEEAYHCGASDVRVVYSDPTLKRLKFENESVEHFANHELKSYDVEARMDYVKRGAA
                     NLALISEDPDLMDGIDSQKLQAFQQQNARAFKGYMESVQKNQFPWVVAAFPSKAWAKR
                     VYPELSVEEAYIKFIDEVFDIVRIDGNDPVENWRQHIANLSVYAQKLQQKNYHALHYV
                     SEGTDLTVGLAKNHIWEDATSYVNGKEQAFIANIPTEEVFTAPDRNRVDGYVTNKLPL
                     SYNGTIIDQFKLMFKDGEIIDFSAEKGETVLKDLINTDEGSRRLGEVALVPDDSPISN
                     RNTIFYNTLFDENAACHLAIGSAYAFNIQGGTEMTVEEKIASGLNDSNVHVDFMIGSS
                     DLTIYGIFEDGSKELVFENGNWASTF"
     gene            12565..13095
                     /locus_tag="NBH32_001106"
     CDS             12565..13095
                     /locus_tag="NBH32_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1514724.1"
                     /translation="MTNQDRPMKSMSESKCYKNRQVFPQDTNHHHTMFGGTLMANIDE
                     IAAITAMKHAGAQVVTASTDSVDFLKPIKTGDILQYVAMVSYAGTSSMEVVVQIRIDD
                     VFNNKHDLAALSYLTFVALDDEGKPKHVPGVYPEDDVEKWFYDTAPQRVERRKARRIE
                     SKQTIEYLAQVQHIRD"
     gene            complement(13355..14506)
                     /gene="yfkAB"
                     /locus_tag="NBH32_001107"
     CDS             complement(13355..14506)
                     /gene="yfkAB"
                     /locus_tag="NBH32_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM/CxCxxxxC motif protein YfkAB"
                     /protein_id="HCD1514725.1"
                     /translation="MNSVKLKQPVSIYNDPWESYNDVKEHGQLTLSNIEFTTTNLCNM
                     RCSHCAVGYTLQTVDPEPLDMDLIYRRLDEIPNLRTMSITGGEPMFSKKSIRNVVKPL
                     LKYAHHRGIYTQMNSNLTLPQDRYLDIAEYIDVMHISHNWGTTDEFANVGFGAMKKQP
                     PLKAKLKLYEQMISNARTLSEQGMFVSAETMLNQSTLPHLRKIHQEVVHGMKCSRHEI
                     HPMYPADFASQLNVLTLAEMKKTIHDILDFRDEDIWMLFGTLPVFPCLKDDEDQKLLS
                     RLRNAKNVTTRNDPDGRSRLNVNVFTGNVIVTDFGDETGTISNIQKDKLTDVFDKWLS
                     SDLAKSLNCHCSEFSCLGPNVLVKNMYYPNMDFKDNERHMHKQPQIIQF"
     gene            14730..14891
                     /locus_tag="NBH32_001108"
     CDS             14730..14891
                     /locus_tag="NBH32_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514726.1"
                     /translation="MNEQQTIEQIKARLNKFIEDIDHVNPDEVRVEDIDEWIGLLDQL
                     EEKVKLVSK"
     gene            15022..15537
                     /locus_tag="NBH32_001109"
     CDS             15022..15537
                     /locus_tag="NBH32_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HCD1514727.1"
                     /translation="MTKKVAIILANEFEDIEYSSPKEALENAGFNTVVIGDTANSEVV
                     GKHGEKVTVDVGIAEAKPEDYDALLIPGGFSPDHLRGDTEGRYGTFAKYFTKNDVPTF
                     AICHGPQILIDTDDLKGRTLTAVLNVRKDLSNAGAHVVDESVVVDNNIVTSRVPDDLD
                     DFNREIVKQLQ"
     gene            15878..16687
                     /gene="sgtB"
                     /locus_tag="NBH32_001110"
     CDS             15878..16687
                     /gene="sgtB"
                     /locus_tag="NBH32_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional peptidoglycan glycosyltransferase
                     SgtB"
                     /protein_id="HCD1514728.1"
                     /translation="MKRSDRYSNSNEHFEHMKHEPHYNTYYQPVGKPPKKKKSKRILL
                     KILLTILIIIALFIGIMYFLSTRDNVDELRKIENKSSFVSADNMPEYVKGAFISMEDE
                     RFYNHHGFDLKGTTRALFSTISDRDVQGGSTITQQVVKNYFYDNDRSFTRKVKELFVA
                     HRVEKQCNKNEILSFYLNNIYFGDNQYTLEGAANHYFGTTVNKNSTTMSHITVLQSAI
                     LASKVNAPSVYNINNMSENFTQRVSTNLEKMKQQNYINETQYQQAMSQLNR"
     gene            16948..17766
                     /gene="recX"
                     /locus_tag="NBH32_001111"
     CDS             16948..17766
                     /gene="recX"
                     /locus_tag="NBH32_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCD1514729.1"
                     /translation="MPKITKIEVQKKNKERFNLFLDEQFEMGIDIDTLVKFNLKKGQQ
                     LEAADMAEIQKYDHYRIGLNKAIQYLSYKKRTEKEVIQYLQKEEISEQAISEVIEYCY
                     REKLIDHQDYAESLKNTMIRTTDKGPKIYQQKLYQLGIEPNIIEMFTELYREQQELDD
                     IIQIAEKISKTKKGPQNKVKEKVMQSLIQKGFEMETIHAVLNEMDFTQDEAVLDDLLQ
                     RDLEKIYNKNRKKYTQQKLISKTIEGLMRKGYKYDKIKAKLEESGIADGTEEIE"
     gene            17744..18058
                     /locus_tag="NBH32_001112"
     CDS             17744..18058
                     /locus_tag="NBH32_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000435810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhH family protein"
                     /protein_id="HCD1514730.1"
                     /translation="MEQKKLSEMSEPELRHEIQLYKEKMRKAEMNGILNEYDVYQSKV
                     IVAESYLVDRKKIEIGKIYKLTDGSNQYFKVERLKGIFAWGFRFNSDEPEEGLPIALL
                     QL"
     gene            18073..19590
                     /locus_tag="NBH32_001113"
     CDS             18073..19590
                     /locus_tag="NBH32_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514731.1"
                     /translation="MGSSIVLKLLKVTHYYRNKQNKKWYLPFGYDAEDIDLNNISLHI
                     YQGEALGIIGEPESSKALVGQLLAGAIKPDKGKVVCTEDLFYGYIEDQSLIHQTVEAY
                     TAQLVQLFPYEINDHKAEQIIQYAHLGDFKTKPVNHISKAAYAQLLLSIARSSKSNII
                     ILNHVIDYLTPQFMERAIELTNDYIENNLTIVSIGDDIDKISQVSNYIAWFSHGQLRM
                     EGSLKQVIPSFKEHERDRLSLNSKEEIENFDLDWKKNRTRIPEMTYNFKRVERYNHAK
                     PPKFLVRFWTLASGTILGLALMMLLIFNNIGIISITDFTNRATMQNENKDPYGEKLAY
                     GIAFNGSVDMQGDKQVTIPKYSVVTITGENSKNYRVTADNKTYYVSKDKLEYFNPAGL
                     YQTHSFKKLAPYMKSNYSNYYAYFNSQLHKKHSSVIKTLVPDDDNRFVASVTQQPIQL
                     LFNDNNQLYGFVYPIVDKKELKDKFNINNNIWITKVGNGYCIANLKEDKWIYIEL"
     gene            19599..20435
                     /locus_tag="NBH32_001114"
     CDS             19599..20435
                     /locus_tag="NBH32_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514732.1"
                     /translation="MLDNIILYFKNLPHTKRYVTERLKQSWKSFLIVLAACLILIIAS
                     ETLFSFSHLTDVKEVRWLFRIIALIVFAVVMFTIYISYHHYMNDFLVTKLFNISAATP
                     VVIMSILSFIMLVILTMISALVKPVNFETSYIALFYFIVMATIFVGLISVTFGLIRLL
                     TEKINIIFYGVCVLCILLLPIIFIPNPNHVFINHILMLNPMYYIVNGIAQSIIFGISS
                     MENIPYHFYFILFLCLIAAVNFVLARYTTHAIYNKTSKVTQTDNQQDVSNDSTDEADT
                     SS"
     gene            complement(20696..21673)
                     /locus_tag="NBH32_001115"
     CDS             complement(20696..21673)
                     /locus_tag="NBH32_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1514733.1"
                     /translation="MDTATHIAIGVGLTALATQDPAMASTFGATATTLIVGSLIPDGD
                     TVLKLKDNATYISHHRGITHSIPFTILWPILITFLIFTFFSGTNPFHVWMWAQLAVFL
                     HVFVDIFNSYGTQALRPITNKWIQLSVINTFDPIIFTVLCIGIVLWVIGLHPFAVFFP
                     IIALLIIYYMIRFKMRAVIKQQALKAIQQEHHPVKVFVAPTIKFMEWRVAIQTDAHDY
                     VGKAYGRNVVFSDKVERQTLSTDSILWKVKGNKDIRTFLNFSSIYRWQTTTLADGSTE
                     IRLIDLRYLKNDHYSFVAIAHVTNDNVIDHSYIGWVFTEDKLQRKLYAK"
     gene            21825..22862
                     /gene="mutY"
                     /locus_tag="NBH32_001116"
     CDS             21825..22862
                     /gene="mutY"
                     /locus_tag="NBH32_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCD1514734.1"
                     /translation="MYQQSSFKENLIHWFDENQREMPWRQTTNPYYIWLSEVMLQQTQ
                     VKTVIDYYHRFVERFPTVEVLSQASEDEVLKYWEGLGYYSRARNFHTAIKEVYDKYEG
                     LVPKDPDQFKALKGVGPYTQAAVMSIAYNVPLATVDGNVFRVWSRLNDDYRDIKLQST
                     RKSYEQELLPYVTTEAGTFNQAMMELGALICTPKNPLCLFCPVQENCEAFDKGTFEKL
                     PVKSKNVSKKVIEQSVFLIRNNQGQYLLQKRSEKLLHGMWQFPMFESEHARHEMTEKI
                     GHDIQPVETPIFELKHQFTHLTWKIKVYAVSGAINIETLPDDMIWFDLSDRDQYTFPV
                     PMSKIYQFING"
     gene            23164..23706
                     /locus_tag="NBH32_001117"
     CDS             23164..23706
                     /locus_tag="NBH32_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD1514735.1"
                     /translation="MVRESIPKEGENIKIQSYKHDGKIHRVWSETTILKGTDHVVIGG
                     NDHTLVTESDGRTWITREPAIVYFHSEYWFNVICMFREDGIYYYCNLSSPFVCDEEAL
                     KYIDYDLDIKVYPNGKYHLLDEDEYEQHMNQMNYPHDIDIILRRNVDILQQWIEQKKG
                     PFAPDFIKVWKERYKKIRQY"
BASE COUNT         8607 a         3348 c         4188 g         7654 t
ORIGIN      
        1 atttaattga gaataaatat catttttaat tatctaaatg agaatgatta taatttagaa
       61 acactgtatc aatttgtgat tgagaaactt ttttgaagtt gccttgtcat gttataatga
      121 tgcgagtgga aatttgagga ggaaacacat gagacagtgg acggcaatcc atctagcgaa
      181 attggcgcgt aaagcaagta gagcagtagg taaaagagga acagatttac ctggacaaat
      241 cgctagaaaa gtggatacag atatattaag aaaattagca gagcaagttg atgatattgt
      301 atttatcagt ggaacaaatg gtaaaacaac gacttcaaac ttaattggac atactttaaa
      361 agcaaataat attcaaatta tacacaataa tgaaggtgct aatatggctg caggtataac
      421 ttctgcattc atcatgcaat caacacctaa gactaaaatt gcggtaatcg aaattgatga
      481 aggttcgatt ccacgtgtgt taaaagaagt tacaccttca atgatggtat ttactaattt
      541 ctttagagat caaatggatc gcttcggtga aattgatatt atggttaata acattgcaga
      601 gacaattagt aataaaggca tcaaattatt gctaaatgct gatgatccat ttgtgagtcg
      661 tttgaaaatc gcaagtgata cgattgtgta ctatggtatg aaagcacatg cccatgaatt
      721 tgaacaaagt acgatgaatg aaagtagata ttgtccaaac tgtggtcgct tattgcaata
      781 cgattatatt cattataatc aaattggtca ttatcactgt cagtgtggtt tcaaacgaga
      841 gcaagcaaaa tatgaaatat caagttttga tgtggcaccg tttttacatt taaatatcaa
      901 tgatgaaaaa tatgatatga aaattgcagg tgactttaac gcttataacg cgttagcagc
      961 atattcagtt ttaagagagc ttgggttaaa tgaacaaaca attaaaaatg gctttgaaac
     1021 gtatacatca gacaatggtc gtatgcagta ctttaaaaaa gaacgaaaag aagcgatgat
     1081 caatttagct aaaaaccctg caggaatgaa tgcaagttta tcagttggtg aacaattaga
     1141 aggcgaaaaa gtgtatgtta tttcgctaaa tgataacgct gcagatggtc gagatacttc
     1201 atggatttat gatgcagatt ttgaaaaatt atctaagcaa caaattgaag ctatcatcgt
     1261 gacaggtaca cgagcagaag aacttcaatt gcgattgaag ttagcagagg ttgaagtacc
     1321 aattatagtt gagcgtgata tttataaagc aacggcaaag actatggatt ataaaggttt
     1381 cacagttgca ataccaaact atacatcatt agcgcctatg cttgaacaat taaaccgttc
     1441 gtttgaagga ggtcaatcat aatatgcatg aattgactat ttatcatttt atgtcagata
     1501 aattgaattt atacagtgat ataggaaata ttattgcttt aagacaacgt gctaaaaaac
     1561 gaaatattaa agttaatgtc gtagaaatca atgaaacaga aggtattacc tttgatgaat
     1621 gtgatatttt ctttatcggt ggtggaagtg atagagaaca agcattagca acaaaagaat
     1681 taagtaaaat taagacacca cttaaagaag cgattgaaga tggtatgccg ggattaacaa
     1741 tttgtggagg ctatcaattt ttaggaaaaa aatatatcac gcctgatggt acagaattag
     1801 aagggttagg tattttagat ttttatactg aatcaaagac aaaccgatta acaggagata
     1861 ttgttatcga aagtgatact tttggaacta ttgtaggttt tgaaaatcac ggtggtagaa
     1921 catatcatga tttcggtaca cttggtcatg ttacttttgg ttatggtaat aatgatgaag
     1981 ataaaaaaga aggcattcat tataaaaatt tattaggtac ttatttacac ggaccaattt
     2041 tacctaaaaa ttacgaaatc actgattatc tgttagaaaa agcttgtgaa cgtaagggta
     2101 ttccgtttga gcctaaagaa atagataatg aagcggaaat acaagcgaaa caagtattaa
     2161 tagacagagc aaatagacag aagaaatctc gttaactctg aatatcgcat caatggattt
     2221 aatattgata aacgatgaag tttagtaatt aatcatatat gtataaacac acacattatt
     2281 ttggatggaa acaaccaaat tgatgtgtgt ttttttgttc tagtgaataa ttattataca
     2341 atgagtatct atcctagaat tatcaatagt aatggtgatt atgcaacatg aaaaaatgaa
     2401 tgatgaaagg aatttgacga tgaagcccac taaagtgatt ttaagagatg catcttattt
     2461 acatagcaaa acatcgataa catttatttt aaaagatgta gttatcgaag aagataataa
     2521 aatttattat ttcgacacta gtgcaacttt cgaagatcaa gaagttaaat ttgaatttgc
     2581 actttttgat agtgacatgg acaatttgaa acatatggat tacgacaatc ctgttaccga
     2641 aatatatttt atagaacctg atttacattt tacgattatt gattttaatc aagaactgct
     2701 ttgtatttat attgattttg attctggttt aaggcattca aacatggcaa cagaatctgg
     2761 tatttcatta aggataaatg ttgctaaatc agattttact aaatttatta atgaattagc
     2821 ctctttacat taatgattta aatctgatac ataattacaa tcaaaaaaga cagccacatc
     2881 cctccgtagt ttaggcgtgt ggctatattt gagtctgaat atttatgctt gtaattttaa
     2941 aaagggacat gctatatacg ataaaaagag gcggggacat aaatcaatgt tctatgctct
     3001 acgaagttat attggcagta gttgactgaa cgaaaatgcg cttgtaacaa gcttttttca
     3061 attctagtca ggggccccaa caaagagaaa ttggattccc aatttctaca gacaatgcaa
     3121 gttggggtgt gggccccaac acagagaatt tcgaaaagaa attctacagg caatgcgagt
     3181 tggggtggga cgacgaaata aattttacga aaatatcatt tctgtcccac tcccatggtg
     3241 ccaattagca taagatactt aaattaagca tatctgctgt ctagcagtcg ataaatcatt
     3301 agaacttcgt atagtatatg acttttaatt tgattttcac cactaatttc aagtgctttt
     3361 attgtcgaac gtaaagtttc tacagaatca tcttctctct taaaagaacc atcataaaat
     3421 atatctttga tgctactact aatttttagc aatgccattt tttcgtcacc tgaaaagtta
     3481 acacgagtat ttttaggcaa gtaaatgata tttgataaat gagtgataaa caaacgattc
     3541 gtatatgcac gtttagttaa ttgattgagt aatttccaat cacattcttt tttcttatga
     3601 tagcttaatt catcacgttg ataacttatt aacgtttcaa cttgattgtt taaattgaaa
     3661 atatttttat atgctttttc gcttttatca gattgcagtc ttgataagat aagttcttgg
     3721 cagcgattgt aaaataattt atacatcaag gcatctgtct tacttaattt ttcttcgacc
     3781 tgaccataat acttaggtgg aaacaccatg aagttaatta aacctgatgt cacgagtcca
     3841 ataattgctg tcaatgttcg agacaaaaag ttgaatatgt aggcatcatg aatacctgga
     3901 atcatagcta atgatgttag tacagcgaca ttcgtaccaa cttgcaattt gagttttgta
     3961 cagaataaaa tcgtgaacgt tgcactcaat gcatatgtaa aaggtgattg atcgccgaat
     4021 aaatatgtaa ataatactgc aaagcctgca ccaattaccg tagcaggtaa tctacgataa
     4081 cctttaataa gtgatgcctt ggcagttggt tcaattgtga ctacagctgt taaaatggca
     4141 tagatgggtg ttaaatctag tgccatacaa aagacagctg ttaaaaaaat ggcaatacca
     4201 gttttaattg ttctggcacc aattaaatgt ttataccatt gatcgttcat tttttaacct
     4261 ctaatcatcg taaaatctta gcgagcgctt tataataata gtatcgtaca ttggaaaagt
     4321 tcatgtatgt aaaatatttg aaataatcat acttaagcat tactttgatt ttcatataca
     4381 ttaatcaaag ttctgggctc cacaatgtta tattgttagt agttgactga aataaaatgc
     4441 acttgtaaca agcatttttc aattatagtc cggggcccca acacagaagc tgacgaaaag
     4501 tcagcttaca ataatgtgca agttggggtg tgggccccaa catagagaat ttcaaaaaag
     4561 aaattctaca ggcaatgcaa gttggggtgt gggccccaac acagagaatt tcgaaaagaa
     4621 attctacagg caatgcaagt tggggacgat aaagaaatat tttttcttta tcacactatg
     4681 tctcactcac tttccaaaat actaaagtaa catctttagt atatcaaaga atttttgcta
     4741 taataagtta taattatata aaaaaggaac gggataaaat gattgtaaaa acagaagaag
     4801 aattacaagc gttaaaagaa attggataca tatgcgctaa agtgcgcaat acaatgcaag
     4861 ctgcaaccaa accaggtatc actacgaaag agcttgataa tattgcgaaa gagttatttg
     4921 aagaatacgg tgctatttct gcgccaattc atgatgaaaa ttttcctggt caaacgtgta
     4981 ttagtgtcaa tgaagaggtg gcacatggga ttccaagtaa gcgtgtcatt cgtgaaggag
     5041 atttagtaaa tattgatgta tcggctttga agaatggcta ttatgcagat acaggcattt
     5101 catttgtcgt tggagaatca gatgatccaa tgaaacaaaa agtatgtgac gtagcaacga
     5161 tggcatttga gaatgcaatt gcaaaagtaa aaccgggtac taagttaagt aacattggta
     5221 aagcggtgca taatacagct agacaaaatg atttgaaagt cattaaaaac ttaacaggtc
     5281 atggtgttgg tttatcatta catgaagcac cagcacatgt acttaattac tttgatccaa
     5341 aagacaaaac attattaact gaaggtatgg tattagctat tgaaccgttt atctcatcaa
     5401 atgcatcatt tgttacagaa ggtaaaaatg aatgggcttt tgaaacgagc gataaaagtt
     5461 ttgttgctca aattgagcat acggttatcg tgactaagga tggtccgatt ttaacgacaa
     5521 aaattgaaga agaatagttc aacatatact aagactaaag tatgaacatc atttagttcc
     5581 ggagcctatt catattggtt tcggaactgt tttataataa ttaagaacac aatcaatgcg
     5641 tcatttcaaa aatatgttgt aataaagtag tttttaagca aacatatcat cgacatcaac
     5701 gaagatacat agcgcatttg gtattttaaa acttattata aaaggtgata gttatgaact
     5761 atgttgaacg ttatattgaa cagtttttga gagcaacagt aagaaataat atcaagcact
     5821 accttttaat gctagatgaa aaaatgaaaa atttagatga ttatatgcgt tatttaatta
     5881 ctaaaaaaga acaacttagc aagttaattg acagtctaat gctaacatta gaaaataaat
     5941 atattgatat tgctgaagca tttcaaattc aatgtgcaag agaaatcaat aatcaagaaa
     6001 ttgaaaatat taaatcagag ttgaataaag ttgaagcata ttatgcacaa attgaaactc
     6061 aaattcaaca aacttcaact gaaaaaatag caacagaaaa aacatcgtat ctaataaatt
     6121 atatgaacgc tgtggcatag aaaggcggcg aaacatgaca cacaaatata tatcaacgca
     6181 aatgttgatt atttttactg cattaatgat tattgccaat ttttactaca tattttttga
     6241 aaaaattggc tttttactcg ttctattatt gggatgtgta ttagtttatg taggatatct
     6301 ttattttcat aaaatacgtg gccttttggc gttttggata ggcgcgctat taattgcatt
     6361 cacattattg tctaataagt atacaatcat catcttgttc gtctttttat tactacttat
     6421 tgtgcgttat ttaatacaca aatttaaacc aaaaaaagta gttgcgacgg atgaggttat
     6481 gacttcacca tcttttatta aacaaaagtg gtttggtgag caacgcacac cagtttatgt
     6541 atataagtgg gaagatgtcc aaatccaaca tggaattggc gacctacata ttgacttaac
     6601 aaaagctgca aatattaagg aaaataatac cattgttgtg agacacattt taggtaaagt
     6661 acaggttata ttgccggtta attacaatat taatttacat gtagctgctt tttatggaag
     6721 tacttacgtg aatgaaaaat catataaagt tgaaaataac aatattcata ttgaagaaat
     6781 gatgaaaccg gataactata cagttaatat ctacgtatca acgtttatcg gagacgtaga
     6841 ggtgatttat cgatgaacca ctacattaga acaattggtt caatgctcat cttagtatat
     6901 agcatgctag ctgcatttct attcatcgat aaagtttttg taaatatcat ctattttcaa
     6961 ggtatgtttt atacacaaat attcggaata ccagtctttt tatttttaaa tctcatcatc
     7021 atattattgt gtattattgt tggttcggta ctcgcttaca aaatcaatca gcaaaatgat
     7081 tggattaaga cgcaaattga gcgttcaatg gaaggcgaaa cagttggcat taatgatcaa
     7141 aatatagaaa tatatagtga aacgttagat ttataccata cactcgtacc tttaaatcaa
     7201 gaattgcata agttgcgact taaaactcaa aacttaacca atgaaaatta taatattaat
     7261 gatgtgaaag ttaaaaagat tattgaagat gaacgtcaaa gactagcacg agaacttcac
     7321 gattctgtta gtcagcaact ttttgcggca agtatgatgc tatctgctat caaagaaacg
     7381 aagttagaac caccattaga ccaacaaatt cctattttag agaaaatggt tcaagattcg
     7441 cagttagaaa tgcgtgcttt gctgttacat ttaagaccgc ttggtttaaa agacaaatct
     7501 ttaggtgagg gtattaaaga tttagttatt gatttacaaa aaaaagtgcc aatgaaagtt
     7561 gtgcatgaaa tacaagattt taaagtgcct aaaggtattg aagatcattt gttcagaatt
     7621 acacaggaag caatttcgaa tacattgcgt cattcaaacg gtacaaaagt gacagtagaa
     7681 ttgtttaata aagacgatta tttattgttg agaattcaag ataatggtaa aggttttaat
     7741 gttgatgaaa aattagaaca aagttatgga cttaaaaata tgcgtgaaag agctttggaa
     7801 attggtgcaa cgttccatat tgtatcattg ccagattcag gtacacgtat cgaggtgaaa
     7861 gcacctttaa ataaggagga ttcgtatgac gattaaagta ttgtttgtgg atgatcatga
     7921 aatggtacgt ataggaattt caagttatct atcaacgcaa agtgatattg aagtagttgg
     7981 tgaaggcgct tctggtaaag aagcaattgc caaagcccat gagttgaagc cagatttaat
     8041 tttaatggat ttacttatgg aagacatgga tggtgtagaa gcgacgactc agattaaaaa
     8101 agatttaccg caaattaaag tattaatgtt aactagtttt attgaagata aagaggtata
     8161 tcgtgcatta gatgcaggtg tcgatagtta cattttaaaa acaacaagtg caaaagatat
     8221 cgccgatgca gttcgtaaaa cttctagagg agaatctgtt tttgaaccgg aagttttagt
     8281 gaaaatgcgt aaccgtatga aaaagcgcgc agagttatat gaaatgctta cagaacgaga
     8341 aatggaaata ttattattga ttgcgaaagg ttactcaaat caagaaattg ctagtgcatc
     8401 gcatattact attaaaacgg ttaagacaca tgtgagtaac attttaagta agttagaagt
     8461 gcaagataga acacaagctg tcatctatgc attccaacat aatttaattc aatagttcgt
     8521 atcgaattaa gaaaagttac ttacgccaat cacaatataa catcaaatag acaccaaaaa
     8581 acagaggtct tcgcaatcat tagcgaaaac ctctgttttg ttggtgttta gtctttttta
     8641 agcttatcaa cgattttgtc ttttatattt ttatcatctt ctggataggt atcactagga
     8701 tgttcatcaa catgatacgt acgttgatgt gttgcattac tatcttcatc atattcgtat
     8761 ctagtgttct cattataatg atttttatta ttgtcatcat gttctaatgc tgcttcttca
     8821 ggtgttttac ctttaattac actacgctga tgaatgattg cattgatttc agcaccgaca
     8881 attataataa aacttgtgat atataaccat agtaataaaa tgatgatacc cgcgatactg
     8941 ccatatgttt tagaatagtt accaaaattt gaaatatacc aaccaaaacc aaatgaacca
     9001 gctaaccaaa taattgaagt aaatactgca cctggtaata ctgacttaag cttcgtttta
     9061 acgttaggtg caaccgaata taacacgata aatatgataa atataataat gattggtaac
     9121 acaattctaa taaggttaaa aatccatttc acttgttcgt caaatccaag tggaccgaat
     9181 aggaaatgac taattacaga accaagcgtt ggtaatgcta gagcaactac aaacacaacg
     9241 cccataacta cagtgaagac aacacttagt agttttaaaa cgattccatt acggctatct
     9301 tctacatcgt aagcaacatt gaaagaattc ataattgcag tcattccatt tgaagctgac
     9361 caaattgcta aaatcaaacc gatagataat aagccaccac tggagttttg agttatatca
     9421 ccaattacac tcttaattag agtagatgtt tcagcgggtg cattgcttaa catattagta
     9481 atttgactct gcttaatatt gaaaaatggt aataatgtta ataggaaaag caacataggg
     9541 aacatagcaa gtacgaaatg gtaagtcatt tgcgctgcta gtcctgaggc atcatctttt
     9601 cctatgcgat agatgagata tgataagaaa ttagagtctt tcttatattt tgcaggttta
     9661 tttaatcttg ataagaagaa aacctgatca tcttttttag gtttcttaga ttggaattgt
     9721 tgaggttcaa tatatgtacg atcaacatca attttgggat tacttttatt tttctttttt
     9781 gactcctctt gcgcttcctt aacagaatta aggtattttg aagaagagtg atcctttttc
     9841 gacatgtgca ctccttaacc tttctctatg taatgtttta taaagtaact attagtattt
     9901 atccaattat aaaaaaacta aacaatgata tgctttaaaa atgataatat tatagaaatt
     9961 aattgattta aatctttcat aggctatttt gatttaaaat taaaacaaag gcaatactga
    10021 aacaatgtag tttggccaat cattgtatta tcatttaata atgataacta tgaaaggcgt
    10081 acaccaaatt gttaagtatt accttataaa aacaaaatca tcttagcgtt gattttttct
    10141 attaacaaat gtttctttcg catcctttaa tgaacgttct aattcaggat tattacgacg
    10201 aatttcttca acaacatctt tccagtataa aacttcgtct ttaatcttgc tgactttaga
    10261 aggcttacga gtgcggttac cattttttgc atctttaact gattgaacta aagcttgacg
    10321 tgtagattta tcagctaaac taattgcacc accaattacg gcaccaatta aaataccagg
    10381 aacaaattta ttttccataa aaaactaccc ctctttcaaa tttgcatctt ttacgatgta
    10441 gtctattaaa ttatcacaag atgataatac catgtcgtat acaccttcaa aattattcgt
    10501 gtagtatgga tctggtacat cactctcttc catattacta aattctaaca gtttgaacaa
    10561 ttgtccctta agattaggat tgatagattt aatattatca acgttacttt gatccatagc
    10621 cacaatgtaa tcaaaatcat ctgtcgcttc gaataattca ctaatcatgc catcaaatgg
    10681 aatattgtgt ttgttgagaa ttttttgtgt accttcatga ggcggctctc ctaaattcca
    10741 gctaccagta cctcttgaat gtactttaat atcatgaata tttctgtctt taagtctttg
    10801 tcgcatgatt gcttctgcca ttggagaacg acatatattg ccaagacaga caaatgctac
    10861 atctaccatt ttgattcctc caaactatgt agttatatcc ccattttata gcgactttaa
    10921 acaataagaa agcagattat ataaaattct attaaagttt attaaattgt gatactttga
    10981 taacataact attattagag gtgaacattg tggctatgac aaatgaagag aaagtattag
    11041 ctattagaga gaagttaaat attgttaatc aaggattatt agatcctgaa aaatataaaa
    11101 atgcaaatga agaagaatta acagatatat atgattttgt tcaatcaaga gaaaaattgt
    11161 cgccaagtga agtgacagct attgctgacg ctttaggaca attgcgacac gactaggagt
    11221 gggaattttg actaattaca aagaaaagtt acaacaatac gctgaactat tagttaaggt
    11281 tggtatgaat gttcaaccaa aacagccagt atttattaga tcatcagtag aaacattaga
    11341 attaacgcat ttaattgtag aagaagcata tcattgtggc gcttcagatg tacgtgttgt
    11401 atatagcgat ccaaccttaa aacgcttaaa atttgaaaat gaatctgtgg aacattttgc
    11461 taatcatgag ttaaaatcat atgatgttga agcgagaatg gattacgtta aacgtggcgc
    11521 tgctaattta gcacttatta gtgaagatcc tgatttaatg gatggtatcg atagccaaaa
    11581 attacaagca tttcaacaac aaaacgcacg cgcatttaaa ggatatatgg aaagtgtaca
    11641 aaaaaatcaa tttccatggg tcgtagctgc ttttccatct aaagcttggg cgaagagagt
    11701 gtatcctgaa ctatctgtag aagaagcata tattaagttt attgacgagg tattcgatat
    11761 tgtgcgaatt gatggtaacg atccagttga aaattggcgt caacacatcg ctaatttaag
    11821 tgtttacgct caaaagttac aacaaaaaaa ttaccatgcg ttgcattatg tatctgaagg
    11881 tactgattta acggtaggtt tagcgaaaaa tcatatctgg gaagatgcta caagttatgt
    11941 taacggcaag gaacaagctt ttattgctaa tataccaact gaagaagtat ttacagcacc
    12001 agatcgaaat agagtagatg gatatgttac taataaattg cctttaagtt ataatggcac
    12061 aattatcgat caatttaaat tgatgtttaa agatggagag attattgatt tttcagctga
    12121 aaaaggtgaa acggtattga aagatttaat caatactgat gaaggttcaa gaagattagg
    12181 tgaagtagca ttagtacctg atgattcacc gatatcaaat cgtaatacca ttttttacaa
    12241 tactttattt gatgaaaatg cagcatgtca tttagcgatt ggatctgctt acgcctttaa
    12301 tattcaaggt ggaacggaaa tgactgttga ggaaaagatt gcaagcggat taaatgattc
    12361 aaatgtacat gtcgatttca tgattggtag tagtgatttg actatttatg gcatattcga
    12421 agatggttca aaagaactag tatttgaaaa tggaaattgg gcatcaacat tttaataaat
    12481 gttattttga ggtgctgagt aggaaatgaa acatgtattt aaaggtcaaa tacgtgatta
    12541 aagtatagat tgggagataa aataatgaca aatcaggaca gaccaatgaa atctatgtca
    12601 gaatcaaaat gttataaaaa tagacaagtt ttccctcaag atacgaatca ccatcataca
    12661 atgtttggtg gtacattgat ggctaatatt gatgaaattg cagcaatcac agctatgaaa
    12721 catgctggtg cgcaagtagt taccgcatct acagactcag tagatttctt aaagccgatt
    12781 aaaacagggg acatattaca atacgtagcg atggtttcat acgctgggac tagttcaatg
    12841 gaagtggtcg ttcaaattag aattgacgat gtatttaata acaagcatga tttagctgca
    12901 ttaagttact taacatttgt tgcgttagat gatgaaggca aacctaaaca tgtacctggt
    12961 gtatatccag aagatgatgt tgaaaaatgg ttttatgata cagcaccaca acgtgttgaa
    13021 cgaagaaaag cacgtcgcat tgaaagtaaa caaacaattg aatatttagc tcaagtgcaa
    13081 catattagag actaaaaata taaagacaga ttgttaagga tgaattgaac tgttatgttc
    13141 gaatattcca cctacaatct gtctttttga ttatgcacgt ttatgctttt agttaaaatt
    13201 tacccctttt attatagact attagtccta ttaaatgacc tttattatta aagtagagta
    13261 ctgccagcaa ttaattgttt tttattttat aatagcatta aaatatgcgg agactactta
    13321 tcgataaagt gcttctccgc ataattaagc gtttttaaaa ttgtataatt tgtggttgtt
    13381 tgtgcatatg acgctcatta tctttaaaat ccatattcgg atagtacata tttttaacaa
    13441 gaacatttgg tcctaaacaa ctaaactcgg aacaatgaca atttaatgat ttagcaagat
    13501 cagaggataa ccatttatca aatacatctg ttaatttatc tttttgtata ttcgaaattg
    13561 tacctgtttc atctccgaaa tcagttacga ttacattacc tgtaaataca ttgacattta
    13621 aacgactacg gccatccggg tcatttctag tcgttacatt tttagcattt cttaaacgtg
    13681 atagtaactt ttgatcttca tcatccttta agcatggaaa cacaggcaaa gtaccaaata
    13741 acatccaaat atcttcatct ctgaaatcca atatatcatg aattgtcttt ttcatttccg
    13801 ctagagttaa cacatttaat tgacttgcaa agtcagctgg atacataggg tgaatctcgt
    13861 gtctgctaca tttcatacca tgaacgactt cttgatgtat ttttcgtaaa tgtggtagcg
    13921 tactttgatt gagcattgtt tccgcagata caaacattcc ttgttctgat aatgtacgtg
    13981 cattcgaaat catttgttca tataatttta acttagcttt taacggtggt tgcttcttca
    14041 ttgcgccaaa gccaacattt gcgaattcat cagttgttcc ccagttatgt gagatatgca
    14101 taacatcgat atattcagca atatctaaat aacgatcttg aggcaatgtt aggtttgaat
    14161 tcatttgtgt atatatacct cgatgatgtg catactttaa tagaggttta acaacatttc
    14221 taatagactt tttagaaaac attggttcgc cacctgtaat tgacatcgtt cgcagatttg
    14281 gaatttcatc aagtctacga taaattaagt ccatatctaa aggctcgggg tcgacagttt
    14341 gtaaagtata accaactgca cagtggctac aacgcatatt acaaagattt gtagttgtaa
    14401 attcgatgtt acttaaagtt aattggccat gttctttaac atcgttatat gattcccatg
    14461 gatcattgta aatgctaaca ggttgcttca atttgacgct attcatcgtc ttaaacttcc
    14521 ttctttctat ttggttcata tcggtatgat accacttcgt attatcactc gtaacaaatt
    14581 atctcttaat tttagttaat tgaaaagggg atttttaaaa gtcaaaataa tttgaatgtc
    14641 ataaatatag ctaagattat atcgctttaa cgcaatttct gttaggataa taatgaaaaa
    14701 gaaaacaaaa taaaaggagt taacttttta tgaatgaaca gcaaacaatc gaacagataa
    14761 aagcgcgttt aaataagttt attgaggata tcgatcatgt aaatcctgat gaagtacgtg
    14821 ttgaagatat agatgaatgg attggattgt tagatcagct tgaagaaaag gttaaattag
    14881 tatctaagta aatgccacat aagatgtgtt agggttagat atcgcgacag tcgcttaata
    14941 atttttatat cttgtatgtt taatcactga tttacttgtc tatagtaatt atatcaatga
    15001 tatatagaaa ggtgattttc aatgactaaa aaagtagcaa ttattctagc aaacgaattt
    15061 gaagatatag aatattcaag ccctaaagag gcattagaga atgcaggctt taatactgta
    15121 gtgattggag atactgcaaa tagtgaagtt gttggtaaac acggtgaaaa agttactgtc
    15181 gatgtaggca ttgcagaagc taaaccagaa gattatgatg cattattaat tcctggagga
    15241 ttttcaccag atcatttacg tggagataca gaaggtcgat atggcacatt tgctaaatac
    15301 tttactaaaa atgatgtacc aacatttgcc atttgtcatg ggccacaaat actaatagat
    15361 acagacgatt taaaaggtcg tacgttaaca gcagtattaa atgtacgcaa agatttatca
    15421 aatgcaggcg cacatgtagt tgatgagtca gtagttgtag acaacaatat tgtaacaagt
    15481 cgagtaccag acgatttaga tgattttaat cgagaaatcg ttaaacaatt acaatagaag
    15541 tattgtggtt atcgattgtt aaaatttata tattttcgtt tgaaatgaaa tatggatttt
    15601 agttttaata aatgaataac aaataaaagg tcgtctctat tggcatttaa tagggatggt
    15661 cttttatttt tacaaaatat aacaatattt gacatgatta atatttaaaa ttgcaatgat
    15721 ttatctataa ttttttcaaa aaacaaaaaa gtaagtatga tggcgtatac gattgtaagc
    15781 ccttggactg attttcccaa aaagtgtagc ctatttgtga tagtactggt aaactcaagg
    15841 tatatactaa gtgagtttta aaagaaggag caaacgcatg aaaagaagcg ataggtactc
    15901 aaactcaaat gaacattttg agcatatgaa acacgaacct cactataata cgtattatca
    15961 accagttggc aaaccgccga aaaagaaaaa aagtaaacga atactattga aaatattatt
    16021 aaccattcta atcattatcg cattgtttat tggtatcatg tattttttat ctacacgcga
    16081 taatgtggat gaactaagaa aaattgaaaa taaaagtagt tttgtgtcag ctgataacat
    16141 gccagagtat gttaaaggtg cctttatttc aatggaagat gaacgattct acaatcatca
    16201 tggattcgat ttgaaaggta caactagagc tttattttca acgattagcg acagagatgt
    16261 gcaaggtggt agtaccatta cacaacaagt tgtcaaaaat tatttttatg ataatgatcg
    16321 ttcatttact agaaaagtaa aagaattatt tgtagctcat cgagttgaaa aacaatgtaa
    16381 taagaacgaa attttaagct tttatttaaa taatatttac tttggggata atcaatatac
    16441 gcttgagggc gcagcaaacc attactttgg aacaaccgtg aataaaaata gtacaacaat
    16501 gtctcacata acagttttac aaagcgctat tttagctagt aaagtcaatg cacctagcgt
    16561 atataatatc aataatatgt cagagaattt tacgcaacgt gtaagcacga acttagaaaa
    16621 aatgaagcaa caaaattata tcaatgaaac acaatatcaa caggctatgt cacaattaaa
    16681 tcgttaaata cttgcattca tgtgtaagat gcttggaact ataatgtaga aggtttaatt
    16741 gaaatgtcat aatgataaag ttgaattgtt tctgctttat aaattttaca gatttgcttg
    16801 taatcacact ttcattttaa aagatgatag tgtgattttt tgttagcatc gaaaaatatt
    16861 taaagttaaa catttgctat aggaagtggc ttattgaata ataaaagagt attgtattaa
    16921 aacttgagaa agtaacgggg tgatccagtg ccgaagatta ctaaaataga agttcaaaag
    16981 aaaaacaaag aacgttttaa tcttttttta gacgaacaat ttgaaatggg tatagatatc
    17041 gatacattag tcaaatttaa tttaaaaaaa gggcaacaac ttgaagctgc tgacatggca
    17101 gagattcaaa agtatgatca ttatcgcata ggtttaaaca aagcaatcca atatttatca
    17161 tataaaaaga gaactgaaaa agaagttata caatatttac aaaaagaaga gatatcagag
    17221 caagcgattt ctgaagtgat tgaatattgt tatcgcgaaa agttaatcga ccatcaagat
    17281 tatgcggaaa gtttaaaaaa tacaatgatt cgcacgacag ataaaggacc taaaatttat
    17341 caacaaaaac tatatcaact tggtatagaa ccaaatatca ttgaaatgtt tacagaactt
    17401 tatagagaac aacaggaact tgatgatatt atccaaattg ctgaaaaaat atctaaaaca
    17461 aaaaaagggc cacaaaataa ggttaaagaa aaagtaatgc aatctttaat tcaaaaaggg
    17521 tttgaaatgg aaaccattca tgctgtattg aatgaaatgg attttacaca agatgaagcg
    17581 gttttagacg atttattaca acgagattta gaaaaaattt ataataaaaa tcgaaagaag
    17641 tacacgcaac agaaattaat ttcaaaaact atcgaaggcc ttatgagaaa aggatataaa
    17701 tatgataaaa ttaaagctaa attagaagaa agtggtattg ccgatggaac agaagaaatt
    17761 gagtgagatg tctgagccag aattaagaca cgaaatacaa ctttataaag agaaaatgag
    17821 aaaagctgag atgaacggta ttttaaatga atatgacgtt tatcaaagta aagtaattgt
    17881 tgccgaaagt tacttagtag acagaaaaaa gatagaaatt ggtaaaattt ataaactgac
    17941 tgatggcagt aatcaatatt ttaaagtaga aagattaaaa ggtatttttg cgtgggggtt
    18001 tagatttaat agcgatgaac ccgaagaagg cttacctata gcgttattac aattatagaa
    18061 ggatgaaaat agatgggaag ttcaattgtt ttaaagttac taaaagtaac acactactat
    18121 aggaataaac agaataaaaa atggtattta ccttttggat atgatgctga agatatcgat
    18181 ttaaacaata ttagtttaca tatttatcaa ggagaagcat taggtataat tggtgaacct
    18241 gaatcttcca aagcgttggt aggtcaattg ttggcaggtg caattaaacc tgataaaggt
    18301 aaagtagttt gtactgaaga tttgttctac ggatatattg aagatcaatc gttaattcat
    18361 caaactgttg aagcttatac agcgcagtta gttcaactat ttccatatga aattaatgat
    18421 cataaagctg aacagattat tcaatatgca catttaggcg attttaaaac gaagccggtt
    18481 aaccatatat cgaaagcggc atacgctcaa ttactattaa gtattgcacg ctcatcaaaa
    18541 tcaaatatta ttattttaaa tcatgttatt gactatttaa caccacaatt tatggaacgt
    18601 gcgattgaat taacaaatga ttatattgaa aataatttaa cgattgtgtc aattggtgat
    18661 gatattgata aaatttcaca agtgagtaac tacatagctt ggttttcaca tggtcaatta
    18721 agaatggaag ggtcacttaa acaagttatt ccatctttta aagaacatga acgtgatcga
    18781 ttaagtctaa actcaaaaga agaaattgaa aactttgatt tagattggaa aaagaatcgt
    18841 acaagaatac cagagatgac ctataatttc aaacgtgttg agcgctataa tcatgcaaaa
    18901 ccgcctaagt ttttagtgcg tttttggact ttagcctcag gtaccatttt aggcttagca
    18961 ttgatgatgt tgctcatttt caataatata ggaattattt cgataacaga ttttacgaat
    19021 cgtgctacga tgcaaaatga aaataaagat ccatatggcg aaaagttagc ttatggaatt
    19081 gcttttaatg gcagtgtgga tatgcaaggg gataaacaag tcacaattcc aaaatatagt
    19141 gtagttacaa ttactggcga aaatagtaaa aattatcgtg ttaccgccga taataagact
    19201 tactatgtta gtaaagataa attagaatat tttaacccgg caggtttata tcaaacgcat
    19261 agttttaaaa aattagcacc atatatgaaa tcaaattata gtaattacta tgcatacttt
    19321 aatagtcaat tacataaaaa gcatagttca gttattaaaa ctttagttcc tgatgatgat
    19381 aaccgtttcg ttgcatccgt tacacaacaa ccgatacaat tacttttcaa tgataataat
    19441 cagttatatg gttttgttta tccaattgta gataaaaaag aattaaaaga taagtttaat
    19501 attaacaata acatttggat tactaaagtt gggaatggat attgtattgc caatttgaaa
    19561 gaagacaaat ggatttatat tgaattgtag gtgtaaagat gctagataat attattttat
    19621 attttaaaaa cttaccgcat actaaaaggt atgtaacaga acgtttaaaa caaagttgga
    19681 aaagttttct aatcgtatta gctgcatgtt taatacttat tattgcaagt gaaacgctat
    19741 tttcctttag tcatttgacc gatgttaaag aggtgcgatg gttatttaga attattgcat
    19801 tgattgtttt tgcggtagtg atgttcacaa tttatatctc ttatcatcat tatatgaatg
    19861 actttttagt tactaagtta tttaatattt ccgcagcgac gccagtagtt attatgtcta
    19921 ttttaagttt tattatgcta gtcattttga cgatgattag tgcattggtt aaaccagtaa
    19981 acttcgaaac atcatatata gcgctattct attttattgt gatggcaact atttttgtag
    20041 gattaatatc cgttactttt ggtcttattc gactgttaac tgaaaaaata aatatcatat
    20101 tttatggggt ttgtgtattg tgtatcttat tgttaccaat tattttcata ccaaatccaa
    20161 atcatgtatt tatcaatcac atcctaatgc tgaatccaat gtattacatt gttaatggta
    20221 tagcacaatc tatcatattt ggtatatcaa gtatggaaaa cattccatat catttttact
    20281 ttattttatt cttatgttta atagctgcag taaatttcgt attagcaagg tatacgacac
    20341 acgcaattta taataaaaca tctaaagtga cacaaactga taatcaacag gatgtttcaa
    20401 atgatagtac agacgaggcg gatacttctt catgatgttt agctgacctg ttgtgtaaat
    20461 taatctgtga gaatcagaaa caattagaat atcatctaga tattgttttc ctatactgat
    20521 tttgatattt cgagtcatta aaggtagttt gttagcaaca atataaattt tagattttgt
    20581 aaaagcattc atagttttac attaaaaaac cggacagagt aaaaatcgtt ttgattttca
    20641 ttctgcccgg ttgttcattt tttatagatt aactttagtg aataataact tgaaattatt
    20701 tagcatacag tttacgttgt aacttatctt ctgtaaatac ccagccaata taagagtggt
    20761 ctatgacatt atcgtttgtt acatgtgcaa ttgccacaaa tgaataatga tcatttttta
    20821 aataacgcaa atcaatcaaa cgaatttcag tagaaccatc tgctaacgtt gttgtttgcc
    20881 aacgatagat tgatgaaaag tttaaaaaag tacgtatatc tttattacct ttgactttcc
    20941 ataaaatgga gtctgttgat aatgtttgac gttccacttt atcactaaac accacattcc
    21001 taccatatgc ttttccaaca tagtcatgtg catcagtttg tatcgcgaca cgccattcca
    21061 taaattttat tgttggcgca acaaatactt taacagggtg atgctcttgt tgaattgctt
    21121 ttaaagcttg ttgcttaatt acggctctca ttttaaaacg aatcatgtaa taaatgatta
    21181 gtaaagcgat tataggaaag aagactgcaa atggatgcaa gcctataacc cataatacaa
    21241 taccaataca aagaactgtg aaaataatag ggtcaaatgt gttaatcaca cttaattgaa
    21301 tccatttgtt tgtgataggt ctaagtgctt gtgtaccata agaattgaat atatctacaa
    21361 agacatgtaa aaatactgcg agctgagccc acatccatac atgaaatggg ttggttccac
    21421 taaagaacgt gaatattaaa aatgtaatta aaattggcca tagtattgtg aaagggatgg
    21481 aatgcgtgat acctctatga tgcgaaatat atgttgcatt gtcctttaat ttaagaacag
    21541 tatccccatc aggaattaat gaaccaacga taagggttgt agctgttgca ccaaacgtag
    21601 aagccattgc tggatcttga gttgcaagtg ctgtaaggcc caccccaatt gcgatatgtg
    21661 ttgcagtgtc catatcagtt cactcatttc ataatattat atatactatt ataatccaat
    21721 ttcgcaaata actaaattaa cttacatact aaccatgatt ttaatgtaca ataaaaacat
    21781 tatgagtaaa gacaattaat tttggtgagg aaagggtttt ataaatgtat cagcagtcta
    21841 gttttaaaga aaatttgata cactggtttg atgaaaatca aagagaaatg ccatggcgac
    21901 aaacgaccaa tccgtactat atttggttaa gtgaggtcat gttacaacag acacaagtta
    21961 aaacagttat tgactattat catcgttttg ttgaacgatt tccaactgtg gaagttttga
    22021 gtcaagcttc ggaagatgaa gtgttaaagt attgggaagg cttaggatat tatagtagag
    22081 ctcgaaattt tcatacggcg attaaagaag tttatgataa atatgaaggc ttagttccca
    22141 aagatcctga tcaatttaaa gcgttaaaag gtgttggccc atacacacaa gctgcagtaa
    22201 tgagcattgc atataatgta ccactagcaa ctgtagacgg aaatgttttt cgagtatggt
    22261 ctagattgaa tgatgactat cgagatatta aattacaatc gacgaggaaa tcgtacgaac
    22321 aagaattgtt accgtatgtt actacagaag ctggaacgtt taatcaagct atgatggaat
    22381 taggggcatt aatttgtaca ccaaaaaatc cattatgctt attttgtcca gttcaagaaa
    22441 actgtgaggc atttgataaa ggtacatttg aaaagttacc tgttaaatct aaaaatgtaa
    22501 gtaaaaaagt aattgagcaa tccgtctttt taattagaaa taatcaaggt caatatctgt
    22561 tgcaaaagcg tagcgaaaaa ttattgcatg gtatgtggca atttcctatg tttgaaagtg
    22621 agcatgctag acatgaaatg actgaaaaaa tcggtcatga cattcaacca gtagaaacac
    22681 ctatttttga gttgaagcat caatttactc atttaacatg gaagattaaa gtatacgccg
    22741 tttcaggagc aattaacatt gaaacattac cagatgacat gatttggttt gatttaagtg
    22801 atcgagatca atatacattc cctgtaccta tgtcaaagat ttatcaattt ataaatgggt
    22861 aacattcgat gattggtaat attatcggaa aacatagtga cattaatttt aaaaagtata
    22921 aatgatatct ttaaatctca tagaataaca atgtcaaaaa gttaaagtgt tgatatataa
    22981 aagtgatgat tttatatact ttaaatggga catgtaaatc tctgttgcag tacttagcaa
    23041 tattaccgag tttaattatt aagtagtaaa atcttatttc gtaaaaatat gtatttatat
    23101 aatactattt ctgaaatatg ctataattta taatggttaa attaacagaa ggtggtgtgg
    23161 agtatggtca gagaatccat acctaaagaa ggggaaaaca ttaaaattca gagttataaa
    23221 catgatggta agattcatcg cgtttggtct gaaacaacaa tattaaaggg tactgatcat
    23281 gttgtcattg gtggcaatga tcatacgtta gtgactgaaa gtgacggaag aacttggata
    23341 acacgtgaac cggcaattgt ttactttcac tcagaatatt ggtttaatgt tatctgcatg
    23401 tttagagaag atggaattta ttactattgc aatttgtcat caccatttgt ttgtgatgaa
    23461 gaggcattaa aatatataga ctatgattta gatattaaag tatatccaaa cggaaagtat
    23521 catttattag atgaagatga atatgagcaa catatgaatc aaatgaatta tccgcatgat
    23581 attgatatta ttttaagacg caacgtggac attcttcaac aatggataga acagaagaag
    23641 ggcccattcg cgccagattt tatcaaagtt tggaaagaac gttacaaaaa aattagacag
    23701 tattaagcaa ataaagtgta aatactgatt aaatcattta tattgactta gtgatgtaaa
    23761 gaagtatgat aacttttgct gtcgtgtggg gacacag
//
LOCUS       DAHHOJ010000033        23119 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.033, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000033 DAHHOJ010000000
VERSION     DAHHOJ010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23119)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23119)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23119
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.033"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(260..1258)
                     /gene="sirC"
                     /locus_tag="NBH32_001118"
     CDS             complement(260..1258)
                     /gene="sirC"
                     /locus_tag="NBH32_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirC"
                     /protein_id="HCD1514736.1"
                     /translation="MTEKINKKDNYHLIFALIFLAIVSVVSMMIGSSFIPLQRVLMYF
                     INPNDSMDQFTLEVLRLPRITLAILAGAALGMSGLMLQNVLKNPIASPDIIGITGGAS
                     LSAVVFIAFFSHLTIHLLPLFAVLGGTIAMMILLVFQTKGQIRPTTLIIIGISMQTLF
                     IALVQGLLITTKQLSAAKAYTWLVGSLYGATFKDTIILGMVILAVVPLLFLVIPKMKI
                     SILDDPVAIGLGLHVQRMKLIQLITSTILVSMAISLVGNIGFVGLIAPHIAKTIVRGS
                     YAKKLLMSAMIGAISIVIADLIGRTLFLPKEVPAGVFIAAFGAPFFIYLLLTVKKL"
     gene            complement(1255..2250)
                     /gene="sirB"
                     /locus_tag="NBH32_001119"
     CDS             complement(1255..2250)
                     /gene="sirB"
                     /locus_tag="NBH32_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter permease
                     subunit SirB"
                     /protein_id="HCD1514737.1"
                     /translation="MLLKPKYQIVIAGLCLAIVAILSLMIGNTLVSPGTVIQALFNFD
                     SENDLHDVVTGARASRTIIALLTGAALAVSGLLMQALTRNPIASPGLFGVNAGAVFFV
                     IFSITFIQIQSFKMIVVIAFLGAIVVTVLVVALGMFRQTLFSPHRVILAGAAIAMLFT
                     AFTQGILIMNETDLQGLLFWLSGSVSLRNIWDIPWIIPLVLILILIAFSMAAHINILM
                     TSDDIATGLGQNIKLIKWMIIMLISMLAGISVAVAGSIVFVGLIVPNISKRLLPPNYK
                     YLIPFTALAGAILMIISDIVARIIIKPLESPIGVVTAVIGAIVLIYIMKKGRQRL"
     gene            complement(2266..3258)
                     /gene="sirA"
                     /locus_tag="NBH32_001120"
     CDS             complement(2266..3258)
                     /gene="sirA"
                     /locus_tag="NBH32_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B ABC transporter
                     substrate-binding protein SirA"
                     /protein_id="HCD1514738.1"
                     /translation="MNKVIKMLVVTLAFLLVLAGCSGNSNKQSSDNKDKETTSIKHAM
                     GTTEIKGKPKRVVTLYQGATDVAVSLGVKPVGAVESWTQKPKFEYIKNDLKDTKIVGQ
                     EPAPNLEEISKLKPDLIVASKVRNEKVYDQLSKIAPTVSTDTVFKFKDTTKLMGKALG
                     KEKEAEDLLKKYDDKVAAFQKDAKAKYKDAWPLKASVVNFRADHTRIYAGGYAGEILN
                     DLGFKRNKDLQKQVDNGKDIIQLTSKESIPLMNADHIFVVKSDPNAKDAALVKKTESE
                     WTSSKEWKNLDAVKNNQVSDDLDEITWNLAGGYKSSLKLIDDLYEKLNIEKQSK"
     gene            3489..4469
                     /gene="sbnA"
                     /locus_tag="NBH32_001121"
     CDS             3489..4469
                     /gene="sbnA"
                     /locus_tag="NBH32_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000570808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diaminopropionate biosynthesis protein SbnA"
                     /protein_id="HCD1514739.1"
                     /translation="MIEKSQACHDSLLDSVGQTPMVQLHQLFPKHEVFAKLEYMNPGG
                     SMKDRPAKYIIEHGIKHGLITENTHLIESTSGNLGIALAMIAKIKGLKLTCVVDPKIS
                     PTNLKIIKSYGANVEMVEEPDAHGGYLMTRIAKVQELLATIDDAYWINQYANELNWQS
                     HYHGAGTEIVETIKQPIDYFVAPVSTTGSIMGMSRKIKEVHPNAQIVAVDAKGSVIFG
                     DKPINRELPGIGASRVPEILNRSEINQVIHVDDYQSALGCRKLIDYEGIFAGGSTGSI
                     IAAIEQLITSIEEGATIVTILPDRGDRYLDLVYSDTWLEKMKSRQGVKSE"
     gene            4466..5476
                     /gene="sbnB"
                     /locus_tag="NBH32_001122"
     CDS             4466..5476
                     /gene="sbnB"
                     /locus_tag="NBH32_001122"
                     /EC_number="1.5.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-[(2S)-2-amino-2-carboxyethyl]-L-glutamate
                     dehydrogenase SbnB"
                     /protein_id="HCD1514740.1"
                     /translation="MNREMLYLNRSDIEQAGGNHSQVYVDALTEALTAHAHNDFVQPL
                     KPYLRQDPENGHIADRIIAMPSHIGGEHAISGIKWIGSKHDNPSKRNMERASGVIILN
                     DPETNYPIAVMEASLISSMRTAAVSVIAAKHLAKKGFKDLTIIGCGLIGDKQLQSMLE
                     QFDHIERVFVYDQFSEACARFVDRWQQQRPEINFIATENAKEAVSNGEVVITCTVTDQ
                     PYIEYDWLQKGAFISNISIMDVHKEVFIKADKVVVDDWSQCNREKKTINQLVLEGKFS
                     KEALHAELGQLVTGDIPGREDDDEIILLNPMGMAIEDISSAYFIYQQAQQQNIGTTLN
                     LY"
     gene            5497..7251
                     /gene="sbnC"
                     /locus_tag="NBH32_001123"
     CDS             5497..7251
                     /gene="sbnC"
                     /locus_tag="NBH32_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis protein SbnC"
                     /protein_id="HCD1514741.1"
                     /translation="MQNHTAVNTAQTIILRDLVDALLFEDIAGIVSNSDITKENGQTL
                     LIYERETQQIKIPVYFSALNMFRYESSQPITIEGRASKQPLTADEFWQTIANMNCDLS
                     HEWEVARVEEGLTTAATQLAKQLSELDLASHPFVMSEQFASLKDRPFHPLAKEKRGLR
                     EADYQVYQAELNQSFPLMVAAVKKTHMIHGDTANIDELENLTAPIKEQATDMLHDQGL
                     SIDDYVLFPVHPWQYQHILPNVFAKEISEKLVVLLPLKFGDYLSSSSMRSLIDIGAPY
                     NHVKVPFAMQSLGALRLTPTRYMKNGEQAEQLLRQLIEKDEALAKYVTVCDETAWWSY
                     MGQDNDIFKDQLGHLTVQLRKYPEVLAKNDTQQLVSMAALAANNRTLYQMICGKDNIS
                     KNDVMTLFEDIAQVFLKVTLSFMQYGALPELHGQNILLSFEDGRVQKCVLRDHDTVRI
                     YKPWLTAHQLSLPKYVVREDTPNTLINEDLETFFAYFQTLAVSVNLYAIIDAIQDLFG
                     VSEHELMSLLKQILKNEVATISWVTTDQLAVRHILFDKQTWPFKQILLPLLYQRDSGG
                     GSMPSGLTTVPNPMVTYD"
     gene            7244..8500
                     /gene="sbnD"
                     /locus_tag="NBH32_001124"
     CDS             7244..8500
                     /gene="sbnD"
                     /locus_tag="NBH32_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B export MFS transporter"
                     /protein_id="HCD1514742.1"
                     /translation="MINQSIWRSNFRILWLSQFIAIAGLTVLVPLLPIYMASLQNLSV
                     VEIQLWSGIAIAAPAVTTMIASPIWGKLGDKISRKWMVLRALLGLAVCLFLMALCTTP
                     LQFVLVRLLQGLFGGVVDASSAFASAEAPAEDRGKVLGRLQSSVSAGSLVGPLIGGVT
                     ASILGFSALLMSIAVITFIVCIFGALKLIETTHMSKSQTPNINKGIRRSFQCLLCTQQ
                     TCRFIIVGVLANFAMYGMLTALSPLASSVNHTAIDDRSVIGFLQSAFWTASILSAPLW
                     GRFNDKSYVKSVYVFATIACGCSAILQGLATNIEFLMAARILQGLTYSALIQSVMFVV
                     VNACHQQLKGTFVGTTNSMLVIGQIIGSLSGAAITSYTTPATTFIVMGVVFAVSSLFL
                     ICSTITNQINEHTLMKLWELKQKSAK"
     gene            8490..10226
                     /gene="sbnE"
                     /locus_tag="NBH32_001125"
     CDS             8490..10226
                     /gene="sbnE"
                     /locus_tag="NBH32_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2,3-diaminopropanoate--citrate ligase SbnE"
                     /protein_id="HCD1514743.1"
                     /translation="MQNKELIQHAAYAAIERILNEYFREENLYQVPPQNHQWSIQLSD
                     LETLTGEFRYWSAMGHHMYHPEVWLIDGKSKKITTYKEAIARILQYMAQSADNQTAVQ
                     QHMAQIMSDIDNSIHRTARYLQSNTIDYVEDRYIVSEQSLYLGHPFHPTPKSASGFSE
                     ADLEKYAPECHTSFQLHYLAVHKNVLLTRYVEGKEDQVEKVLYQLADINISEIPKDFI
                     LLPIHPYQINVLRQHPQYMQYSEQGLIKDLGVSGDLVYPTSSVRTVFSKALNIYLKLP
                     IHVKITNFIRTNDLEQIERTIDAAQVIASVKDEVETPHFKLMFEEGYRALLPNPLGQT
                     VEPEMDLLTNSAMIVREGIPNYHADKDIHVLASLFETMPDSSMSKLSQVIEQSGLAPE
                     PWLECYLDRTLLPILKLFSNTGISLEAHVQNTLIELKDGIPDVCFVRDLEGICLSRTI
                     ATEKQLVPNVVAASSPVVYAHDEAWHRLKYYVVVNHLGHLVSTIGKATRNEVVLWQLV
                     AHRLMTWKKEYANNAVFVDCVEDLYQTPTIAAKANLMSKLNDCGANPIYTHIPNPICH
                     NKEVSYCESNNS"
     gene            10135..11985
                     /gene="sbnF"
                     /locus_tag="NBH32_001126"
     CDS             10135..11985
                     /gene="sbnF"
                     /locus_tag="NBH32_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-(L-alanin-3-ylcarbamoyl)-2-[(2-
                     aminoethylcarbamoyl)methyl]-2-hydroxypropanoate synthase
                     SbnF"
                     /protein_id="HCD1514744.1"
                     /translation="MIVVQTLFIHIYQIQFVITRRYRIVNQTILNRVKTRVMHQLVSS
                     LIYENIVVYKASYQDGVGHFTIEGHDSEYRFTAEKTHSFDRIRITSPIERVVGDEADT
                     TTDYTQLLREAVFTFPKNDEKLEQFIVELLQTELKDTQSMQYRESNPPATPETFNDYE
                     FYAMEGHQYHPSYKSRLGFTLSDNLKFGPDFVPNIKLQWLAIDKDKVETTVSRNVVVK
                     EMLRQQVGDKTYEHFVQQIEASGKHVNDVEMIPVHPWQFEHVIQVDLAEERLNGTVLW
                     LGESDELYHPQQSIRTMSPIDTTKYYLKVPISITNTSTKRVLAPHTIENAAQITDWLK
                     QIQQQDTYLKDELKTAFLGEVLGQSYLNTQLSPYKQTQVYGALGVIWRENIYHMLIDE
                     EDAIPFNALYASDKDGVPFIEKWIKQYGSEAWTKQFLAVAIRPMIHMLYYHGIAFESH
                     AQNMMLIHENGWPTRIALKDFHDGVRFKREHLSEAASHLTLKPMPEAHKKVNSNSFIE
                     TDDERLVRDFLHDAFFFINIAEIILFIEKQYGIDEQRQWKWVKDIIEAYQEAFPELNN
                     YQHFDLFEPTIQVEKLTTRRLLSDSELRIHHVTNPLGVGGINDATTISET"
     gene            11960..12736
                     /gene="sbnG"
                     /locus_tag="NBH32_001127"
     CDS             11960..12736
                     /gene="sbnG"
                     /locus_tag="NBH32_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis citrate synthase
                     SbnG"
                     /protein_id="HCD1514745.1"
                     /translation="MQQLSLKHRLNNGDSVYGIFNSIPDPLMIEVIAASGYDFVVIDT
                     EHVAINDETLAHLIRAAEAAHIIPIVRVTAVIDRDIIKVLDMGARGIIVPHVKDRETV
                     EHIVKLSRYYPQGLRSLNGGRMARFGRTPLLDAMEMANEHIMVIAMIEDVEGVMAIDD
                     IAQVEGLDMIVEGAADLSQSLGIPWQTRDDQVTSHVQHIFEIVNAHGKHFCALPREDE
                     DIAKWQAQGVQTFILGDDRGKIYRHLSASLATSKQKGDEG"
     gene            12736..13938
                     /gene="sbnH"
                     /locus_tag="NBH32_001128"
     CDS             12736..13938
                     /gene="sbnH"
                     /locus_tag="NBH32_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphyloferrin B biosynthesis decarboxylase
                     SbnH"
                     /protein_id="HCD1514746.1"
                     /translation="MRIVQPVIEQLKAQSHPVCHYIYDLVGLEHHLQHITSSLPSNCQ
                     MYYAMKANSERTILDTISQYVEGFEVASQGEIAKGLAFKPANHIIFGGPGKTDEELRY
                     AVSEGVQRIHVESMHELQRLNAILEDEDKTQHILLRVNLAGPFPNATLHMAGRPTQFG
                     ISEDEVDDVIEAALAMPNIHLDGFHFHSISNNLDSNLHVDVVKLYFKKAKAWSEKHRF
                     PLKHINLGGGIGVNYADLTSQFEWDNFVERFKTLIVEQEMEDVTLNFECGRFIVAHIG
                     YYVTEVLDIKKVHGAWYAILRGGTQQFRLPVSWQHNHPFEIYRYKDNPYSFEKVSISR
                     QDTTLVGQLCTPKDVFAREVKIDAISTGDVIVFKYAGAYGWSISHHDFLSHPHPEFIY
                     LTQTKEDE"
     gene            13942..14706
                     /gene="sbnI"
                     /locus_tag="NBH32_001129"
     CDS             13942..14706
                     /gene="sbnI"
                     /locus_tag="NBH32_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transcriptional
                     regulator/O-phospho-L-serine synthase SbnI"
                     /protein_id="HCD1514747.1"
                     /translation="MNHIHEHLKLVPVDKVDLHETFEPLRLEKTKSSIEADDFIRHPI
                     LVTAMQHGRYMVIDGVHRYTSLKALGCKKVPVQEIHETQYSISTWQHKVPFGVWWETL
                     QQEHRLPWTTETRQEAPFITMCHGDTEQYLYTKDLGEAHFQVWEKVVASYSGCCSVER
                     IAQGTYPCLSQQDVLMKYQPLSYKEIEAVVHKGETVPAGVTRFNISGRCLNLQVPLAL
                     LKQDDDVEQLRNWKQFLADKFANMRCYTEKVYLVEQ"
     gene            14902..15309
                     /locus_tag="NBH32_001130"
     CDS             14902..15309
                     /locus_tag="NBH32_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514748.1"
                     /translation="MSSIIGRITIWIGIVAQIYFSVVFVRMISINIAGGSDYETIFLL
                     GLILTLFTVLPTIFTAIYMESYSVIGGALFIVYAIIALCLYNFLSAILWLIGGILLIW
                     NKYSKDESTDENEKVDIESTENQFESKDKITKE"
     gene            15359..15985
                     /locus_tag="NBH32_001131"
     CDS             15359..15985
                     /locus_tag="NBH32_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1514749.1"
                     /translation="MEKNVEKSFIKIGLYFQIAYIVLMAITLCGFVICYGLIFGLFYL
                     LSGSRADYLIVTIVISAIISIFVIILSIVPVIVLASDLFKERISKGVILIVLAIIALV
                     LCNFVSAILWFVSAISILGRKKLVAAADTTTIQKSKGNANQASHKDTGKKELDSQDMM
                     EHPEFKNPTTKNLEGLNEEIHKDEATTKVDSDNTQPPIESKDHVSKKD"
     gene            16196..16972
                     /locus_tag="NBH32_001132"
     CDS             16196..16972
                     /locus_tag="NBH32_001132"
                     /EC_number="1.1.1.304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-acetoin forming diacetyl reductase"
                     /protein_id="HCD1514750.1"
                     /translation="MTNNKVALVTGGAQGIGFKIAERLVEDGFKVAVVDFNEEGAKAA
                     ALKLSSDGTKAIAIKADVSNRDDVFNAVRQTAAQFGDFHVMVNNAGLGPTTPIDTITE
                     EQFKTVYGVNVAGVLWGIQAAHEQFKKFNHGGKIINATSQAGVEGSPGLSLYCSTKFA
                     VRGLTQVAAQDLASEGITVNAFAPGIVQTPMMESIAVATAEEAGKPEAWGWEQFTSQI
                     ALGRVSQPEDVSNVVSFLAGKDSDYITGQTIIVDGGMRFR"
     gene            17116..17238
                     /locus_tag="NBH32_001133"
     CDS             17116..17238
                     /locus_tag="NBH32_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514751.1"
                     /translation="MHQLKALLVLTHPRYYKTTQKRHYLIYLNKNSQSYLILFL"
     gene            17308..18294
                     /locus_tag="NBH32_001134"
     CDS             17308..18294
                     /locus_tag="NBH32_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCD1514752.1"
                     /translation="MEDLERVLITGGAGFIGSHLVDDLQQDYDVYVLDNYRTGKRENI
                     KNLADDHVFELDIREYDAVEQIMKTYQFDYVIHLAALVSVAESVEKPILSQEINVVAT
                     LRLLEIIKKYNSHIKRFVFASSAAVYGDLPDLPKSDQSLILPLSPYAIDKYYGERTTL
                     NYCSLYNIPTAVVKFFNVFGPRQDPKSQYSGVISKMFDSFEHNKPFTFFGDGLQTRDF
                     VYVYDVVQSVRLIMEHKDAIGHGYNIGTGTFTNLLEVYRIIGELYGKSVEHEFKEARK
                     GDIKHSYADISNLKALGFVPKYTVETGLKDYFNFEVDNIEEVTAKEVEMSKK"
     gene            18251..18943
                     /locus_tag="NBH32_001135"
     CDS             18251..18943
                     /locus_tag="NBH32_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HCD1514753.1"
                     /translation="MKKLQLKKWKCQKNNIEAVHNNKGYAYQRKLDKLEEVRKRYYPI
                     KRAIDLILSIVLLFLTLPIMVIFAIAIVIDSPGNPIYSQVRVGKMGKLIKIYKLRSMC
                     KNAEKNGAQWADKDDDRITNVGKYIRKTRIDELPQLINVVKGEMSFIGPRPERPEFVE
                     LFSSEVIGFEQRCLVTPGLTGLAQIQGGYDLTPQQKLKYDMKYIHKGSLMMELYISIR
                     TLMVVITGEGSR"
     gene            19153..20319
                     /locus_tag="NBH32_001136"
     CDS             19153..20319
                     /locus_tag="NBH32_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCD1514754.1"
                     /translation="MKIIYCITKADNGGAQTHLIQLANHFCVHHDVYVIVGNHGPMIE
                     QLDARVNVIILEHLVGPIDFKQDILAVKVLAQLFSKIKPDVIHLHSSKAGTVGRIAKF
                     ISKSKDTRVVFTAHGWAFTEGVKPAKKFLYLVIEKLMSRITDSIICVSDFDKQLALKS
                     RFNRLKLTTIHNGIADVPAVKQTLKSQSHNNIGEVVGMLPNKQDLQINAPTKHQFVMI
                     ARFAYPKLPQNLIAAIEILKLHNSNHAHFTFIGDGPTLNDCQQQVVQAGLENDVTFLG
                     NVINASHLLSQYDTFILISKHEGLPISIIEAMATGLPVIASHVGGISELVTDNGICMM
                     NNQPETIAKVLEKYLIDSDYIKMSNQSRKRYLECFTEEKMIKEVEDVYNGKSTQ"
     gene            20300..21538
                     /locus_tag="NBH32_001137"
     CDS             20300..21538
                     /locus_tag="NBH32_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD1514755.1"
                     /translation="MENQHNSKYLTLLLIGLAVFIQQSSVVAGVNVSIADFITLLILV
                     YLLFFANHLLKANHFLQFFIILYTYRMIITLCLLFFDDLIFITVKEVLASTVKYAFVV
                     IYFYLGMIIFKLGNSKKVIVTSYIISSVTIGLFCIIAGLNKSPLLMKLLYFDEIRSKG
                     LMNDPNYFAMTQIITLVLAYKYIHNYIFKVLACGILLWSLTTTGSKTAFIILIVLAIY
                     FFIKKLFSRNAVSVVSMLVIMLILLCFTFYNINYYLFQLSDLDALPSLDRMASIFEEG
                     FASLNDSGSERSVVWINAISVIKYTLGFGVGLVDYVHIGSQINGILLVAHNTYLQIFA
                     EWGILFGALFIIFMLYLLFELFRFNISGKNVTAIVVMLTMLIYFLTVSFNNSRYVAFI
                     LGIIVFIVQYEKMERDRNEE"
     gene            21528..22958
                     /locus_tag="NBH32_001138"
     CDS             21528..22958
                     /locus_tag="NBH32_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCD1514756.1"
                     /translation="MKSDSLKENIIYQGLYQLIRTMTPLITIPIISRAFGPSGVGIVS
                     FSFNIVQYFLMIASVGVQLYFNRVIAKSVNDKRQLSQQFWDIFVSKLLLALTVFAMYI
                     AVITIFIDDYYLIFLLQGIYIIGAALDISWFYAGTEKFKIPSLSNIVASGIVLSVVVI
                     FVKDQSDLSLYVFTIAIVTVLNQLPLFIYLKRYISFVSVNWIHVWQLFRSSLAYLLPN
                     GQLNLYTSISCVVLGLVGTYQQVGIFSNAFNILTVAIIMINTFDLVMIPRITKMSLHQ
                     SHSLTKTLADNMNIQLILTIPMVFGLIAIMPSFYLWFFGEEFASTVPLMTILAILVLI
                     IPLNMLISRQYLLIVNKIRLYNASITIGAVMNLVLCLVLIYFYGIYGAAIARLITEFF
                     LLIWRFIDITKINVKLNIVSTIQCVIAAVMMFIVLGVVNHYLPPTMYATLLLIAIGIV
                     VYLLLMMTMKNQYVWQILRHLRHKTI"
BASE COUNT         7710 a         3563 c         4484 g         7362 t
ORIGIN      
        1 gtttttgaat aaatgacatt atcaaacact atatttaaat aataaatagt tatcgatatt
       61 tgaaaaatcg aagaatattg attgatatta tagaatatga gtcggtgtta tttgtatata
      121 aagtattaat atgtaattaa gaaagtcgac gcttgttaac attaacaaca actatatgta
      181 taaaaaaacc tcgcaactgt tagttaactg aatcatttag ctaacttcgt tgtgaggtca
      241 ttttgttttg ataatatctt tataactttt tcacggttaa taataagtat atgaagaatg
      301 gggcaccaaa agcagcaata aatacacctg ctggcacttc tttaggcaag aataaggtac
      361 gcccaattaa gtctgcaata acaattgata tggcaccaat cattgctgac attagtaact
      421 ttttagcata acttccgcga acgattgttt tcgcgatatg tggtgcgatt aaaccgacaa
      481 acccaatgtt acctactaaa ctgattgcca tagatacgag tatagtagaa gtgattaatt
      541 ggattagttt catacgttgt acatgtaagc ctaagccaat cgctacaggg tcatcaagta
      601 tagatatttt catttttggt ataacaagaa ataacaacgg cacaacagct aaaataacca
      661 tacccaaaat gattgtatct ttaaacgtag caccgtaaag acttccgact agccatgtat
      721 aagctttggc agcagataat tgcttcgttg taatgagtaa tccttggaca agcgcaataa
      781 acaacgtttg catcgaaata ccgatgatta tgagtgttgt cgggcgtatt tgtcctttcg
      841 tttgaaacac taatagtatc atcattgcaa ttgtgccacc taatacagcg aatagtggaa
      901 gtaaatgtat cgttaaatgg ctgaaaaatg caataaagac aacagcactt aagctagcac
      961 cacctgtgat accgataata tcaggtgagg caattggatt ttttaataca ttttgcaaca
     1021 ttaaaccact cattcctagt gcggcacctg ctaaaatcgc aagtgtaatg cgaggtaagc
     1081 gtaatacttc taaagtgaat tgatccatac tgtcatttgg atttataaag tacatcagta
     1141 cgcgttgtaa tggtataaag cttgaaccaa tcatcatact taccactgaa acgatggcta
     1201 aaaagattaa cgcgaagatg agatggtaat tgtctttttt attaatcttt tcggtcataa
     1261 gcgttgacgt cctttcttca taatatagat taagacaata gcgccaatga cagcggtaac
     1321 gacaccgata ggcgactcta gtggcttaat tattatacga gcaacaatgt ctgaaatgat
     1381 cattaggatt gctccagcta atgcagtaaa aggaattaaa tacttatagt ttggtggtaa
     1441 taatcgtttg ctaatattcg gtacgataag acccacaaag acgattgatc cagctacggc
     1501 taccgaaata ccggctaaca tactgatgag cataataatc atccatttga ttaattttat
     1561 gttttgaccg aggccggttg caatgtcgtc acttgtcatc aagatgttga tgtgtgcagc
     1621 catgctaaat gcaattaaaa taagtatcaa tacaagcgga ataatccatg ggatatccca
     1681 aatgttacgt aatgaaacgg agccactcaa ccaaaataat aggccttgta agtctgtttc
     1741 gttcataata agtatgcctt gagtaaaggc tgtaaatagc atcgcaatcg cagcacctgc
     1801 caaaatgaca cggtgaggtg agaatagtgt ttgtctaaac atacctagcg caacgactaa
     1861 gactgtaaca acaatagccc ccaaaaatgc gatgacaaca atcattttaa aagattgaat
     1921 ttggataaat gtaatactaa agatgacaaa aaatactgcg cctgcattga caccgaaaag
     1981 gcctggtgat gctattgggt ttcgtgtaag tgcttgcatc aacaaacctg agacagcaag
     2041 ggcagcacca gtcaataacg caatgattgt tctcgacgcc cgtgcaccag tgacaacatc
     2101 atgtaaatcg ttttcactat caaagttgaa taacgcctgt atcaccgtac ctggtgacac
     2161 aagcgtattt ccaatcatta aacttaagat agctactatt gcaagacata aaccagcaat
     2221 aacgatttgg tattttggtt taagtagcat cgtaaaactc cttaattatt ttgattgttt
     2281 ttcaatattt aacttttcat ataaatcgtc aatcaatttt aatgatgatt tatatccgcc
     2341 agctaagttc caagtgattt catctaaatc atcagatact tggttgtttt taactgcgtc
     2401 taaatttttc cactctttac ttgaagtcca ttcgctttca gtctttttaa ctaatgcagc
     2461 atctttcgca tttggatctg attttactac aaaaatatga tcagcgttca ttaacggaat
     2521 gctttcttta gatgtaagtt ggataatatc tttaccatta tcaacttgtt tttgtaagtc
     2581 tttattacgt ttgaatccta aatcatttaa gatttcacca gcatatccac cagcataaat
     2641 tcttgtatga tcagcacgga agttaacaac tgaagctttc aatggccatg catctttata
     2701 ctttgctttt gcatcttttt ggaatgcagc tactttatca tcgtactttt taagtaaatc
     2761 ttcagcttct ttttctttcc ctaaagcttt ccccattaac ttagttgtat ctttgaattt
     2821 gaaaactgta tcagtagaaa ctgttggtgc gattttagat aattgatcgt aaactttttc
     2881 atttctaact tttgacgcga caattaagtc cggttttaat ttagagattt cttctaagtt
     2941 aggtgcaggt tcttgaccta caatcttagt atcttttaaa tcatttttta tgtattcgaa
     3001 tttcggtttt tgtgtccatg attctacagc acctacaggt ttaacaccta aagatacagc
     3061 gacgtcagtg gcaccttgat atagcgtaac aacacgcttt ggtttccctt taatttcagt
     3121 tgtacccatt gcatgtttaa ttgaagttgt ttccttatct ttgttatcag atgattgctt
     3181 atttgaattc ccactacatc ctgctaaaac aagtaggaaa gcaagcgtaa caacaagcat
     3241 tttaattact ttattcattg actaattagc ctccttcgtg atgtatgaca atgagaatca
     3301 ttatcacgat ttagtatgaa ttaaaatttt tcctaagtca ataaaatatt tatgatttac
     3361 atgcaactta taattatttg acatataaat gcataaaaaa tataatccta attacttgat
     3421 agtgagaata attatcaatt aggtaacaca caatattata gaattttaaa tttgaggagg
     3481 aagcgctttt gattgaaaaa agtcaagcat gtcacgattc attgttagat tctgtagggc
     3541 aaacacctat ggttcaactt catcaactat ttccgaaaca tgaagtgttt gcaaagttag
     3601 agtatatgaa tcctggaggc agcatgaaag atcgacctgc caagtacatc attgaacatg
     3661 gtattaaaca tggtttaatc actgagaata cacatttaat tgaaagtact tctggtaatt
     3721 taggcattgc gttggcaatg atagctaaaa tcaagggatt aaaactcacg tgtgttgttg
     3781 atcctaaaat atcaccaaca aatttgaaaa ttattaaaag ttatggtgcc aatgtagaaa
     3841 tggttgaaga acctgatgca catgggggtt atttaatgac tcgtattgca aaggtgcaag
     3901 aactgttagc cactattgac gatgcatatt ggattaatca atatgcgaat gagttaaatt
     3961 ggcaatccca ttatcatggt gcaggcacag agattgttga aacaattaag caacctatag
     4021 attattttgt cgcgccagtc agcacgacag gtagcattat gggtatgagt agaaaaataa
     4081 aagaagtgca tccaaacgca caaattgttg ctgttgatgc gaaagggtca gtcatttttg
     4141 gtgacaaacc tattaataga gaattacctg gtatcggtgc tagtcgtgta cccgaaatat
     4201 tgaatagatc agaaattaat caagtgatcc atgtagatga ttatcaatct gctttgggct
     4261 gtcgaaaact gattgattat gaaggcatat ttgccggagg ttcaacaggt tcgattattg
     4321 cagcgattga gcagttgata acgtcaattg aagaaggtgc aacaattgtc acgattttac
     4381 cagatcgagg cgatcgttac ttagatttag tttattcaga tacatggtta gaaaaaatga
     4441 aatcaagaca aggagttaaa tcagaatgaa tagagagatg ttgtatttaa atagatcaga
     4501 tattgaacaa gcgggaggaa atcattcaca agtttatgtg gacgcattaa cagaagcatt
     4561 aacagcgcat gcgcacaatg attttgtaca accgcttaag ccgtatttaa gacaggatcc
     4621 tgaaaatgga cacatcgcag atcgaattat tgcaatgcca agccatatcg gtggagaaca
     4681 cgcaatttca ggtattaagt ggataggtag taagcacgac aatccatcga aacgtaatat
     4741 ggagcgtgca agtggtgtca ttattttgaa tgatccggaa acgaattatc caattgcagt
     4801 tatggaagca agtttaatta gtagtatgcg tactgcagca gtttcagtga ttgcagctaa
     4861 gcatttggct aaaaaaggat ttaaagactt aacaatcatt ggatgcgggc taatcggaga
     4921 caagcaatta caaagtatgt tagagcaatt cgatcatatt gaacgcgtgt ttgtttacga
     4981 tcaattctct gaagcatgtg cacgctttgt tgatagatgg caacaacagc gtccggaaat
     5041 taattttatt gcgacagaaa atgctaaaga agcagtatca aatggtgaag tagtcattac
     5101 atgtaccgta acggatcaac catacattga atatgattgg ttacaaaagg gtgcatttat
     5161 tagcaacatt tctatcatgg atgtgcataa agaagtcttt attaaagctg acaaagtcgt
     5221 agtagatgac tggtcacaat gtaatcgaga aaagaaaact attaaccaat tggtgttaga
     5281 aggtaaattc agcaaagaag cacttcatgc tgaactaggg caacttgtga caggtgacat
     5341 accaggacgt gaagacgatg atgaaatcat attacttaat ccgatgggta tggctatcga
     5401 agatatttca agtgcttatt ttatttatca acaggcacaa caacaaaata ttgggacaac
     5461 attgaaccta tattaagaat gcgaggtgtc tgaacattgc agaatcatac agcagtcaat
     5521 acagcacaaa cgataatatt aagggattta gttgatgcat tattatttga agatatagcc
     5581 ggaattgtat cgaatagtga tattactaaa gaaaatggac aaacgctttt gatatacgaa
     5641 cgtgaaacac aacaaataaa gatacctgtt tattttagtg ctttaaatat gtttcgttat
     5701 gaaagttcac aaccaattac gatagaggga agggcgtcta agcaaccact aactgcagat
     5761 gaattttggc aaacaattgc taatatgaat tgtgatttaa gtcatgaatg ggaagtggct
     5821 cgcgttgaag aaggactgac tactgctgcc acacagcttg ctaaacaatt atcagaatta
     5881 gatttagcgt cacatccttt tgtgatgtca gagcagtttg caagtttaaa agatcgtcca
     5941 tttcatccat tagctaaaga aaaaagagga ttaagagaag cggattatca agtgtatcaa
     6001 gctgaattaa atcaatcatt tcctttaatg gttgcagcag ttaaaaagac acatatgatt
     6061 catggcgata ctgcaaatat tgatgaatta gaaaatttga cagcccctat aaaagaacaa
     6121 gcgacagata tgttacatga tcaagggtta tcaatagatg actatgtact atttcctgta
     6181 catccttggc aatatcaaca tattttgccg aacgtctttg cgaaagagat tagtgaaaaa
     6241 ttggttgtac tattaccgtt aaaatttgga gattatctgt cgtcttcaag tatgcgttca
     6301 ttaattgata ttggcgcacc gtataaccat gtcaaagtac catttgcaat gcagtcatta
     6361 ggcgcattaa gactaacgcc tacgcgttac atgaaaaacg gagaacaagc agaacaatta
     6421 ttacgtcagc ttatagaaaa agatgaagca ctagctaagt atgtcacagt ttgcgatgag
     6481 acagcttggt ggtcatatat gggtcaagac aatgatattt tcaaagatca attaggtcat
     6541 ttaactgttc agctaaggaa atatccagaa gtgctagcca aaaatgatac gcaacagcta
     6601 gtgtcaatgg cagcactcgc ggcaaataat cgcaccttat atcaaatgat ttgtggaaaa
     6661 gataatattt ctaaaaatga tgtcatgacg ttatttgaag atatcgcgca agtcttttta
     6721 aaggtaacac tatcatttat gcaatacggc gcattaccag agttgcatgg tcaaaatata
     6781 ttgttgtcat ttgaagatgg acgtgtacaa aaatgcgtgt tacgtgatca tgatactgtc
     6841 agaatttata aaccatggct aacagcacat cagctttcat tgccgaagta tgtcgtcaga
     6901 gaagatacac ctaatacgct aattaatgaa gatttggaaa cattctttgc ttattttcaa
     6961 acattagctg tatcggtaaa tctatatgcc attattgatg caattcaaga tttatttggt
     7021 gtaagtgagc atgaacttat gtcgttgtta aaacaaattt taaaaaatga agtggcaact
     7081 atttcctggg ttacaactga tcagctagct gtcagacaca ttttatttga taaacagacg
     7141 tggccattca aacaaatttt attaccattg ctatatcaac gtgatagtgg tggaggtagt
     7201 atgccttcag gtttaactac cgtaccaaat ccaatggtga catatgatta atcagtctat
     7261 atggcgcagt aactttcgca ttttatggct cagtcagttt atagcgattg ctggactgac
     7321 agtacttgtg ccattattgc caatttatat ggcatcacta caaaatctat cagtcgtaga
     7381 aatacagttg tggagtggta tagcgattgc tgctcctgct gtaacgacga tgatagcttc
     7441 gccgatatgg gggaagctag gtgataagat cagtcgaaaa tggatggtgt taagagcgtt
     7501 acttggtttg gcggtatgct tatttttaat ggcattgtgt acgacaccat tacagtttgt
     7561 acttgtgagg ttattgcagg gactatttgg tggtgttgtt gatgcatcaa gtgcgtttgc
     7621 gagtgcagag gcgccagctg aagatcgtgg caaggtatta ggaagactgc aaagttcagt
     7681 cagcgcaggg tctcttgtgg ggccattaat tggcggtgtt acagcttcga tattaggttt
     7741 tagtgcgtta ctgatgagta ttgccgttat tacttttatt gtctgtattt tcggtgcatt
     7801 aaaattgatt gaaacgacac atatgtcaaa atcacaaaca ccaaatatta ataaaggtat
     7861 tcgccgttca tttcaatgtc tattatgcac acaacaaaca tgtcgattta ttatcgttgg
     7921 cgttttagca aactttgcta tgtatggcat gctaactgca ttatcaccac ttgcttcatc
     7981 agtgaatcat acagcgatag atgaccgtag tgtgattgga tttttacagt ccgcattttg
     8041 gacggcttcg atattaagcg cgcctttatg gggtcgcttt aatgataaat catatgttaa
     8101 atcagtatat gtatttgcaa cgattgcatg tggttgtagt gcgatactgc aaggtttagc
     8161 gacgaatata gagtttttaa tggctgcaag gatacttcaa ggattaacat atagtgcatt
     8221 gattcaaagt gtcatgtttg ttgtcgtaaa tgcgtgtcat caacaactta aaggcacatt
     8281 tgttggtacg acaaatagta tgttagttat tggccaaatt attggtagtc ttagtggcgc
     8341 tgccattact agttatacta caccagctac tacgtttatc gttatgggcg tagtatttgc
     8401 agtaagtagt ttatttttaa tttgttcaac catcactaat caaatcaacg agcacacatt
     8461 aatgaaatta tgggagttga aacaaaaaag tgcaaaataa agaattaata caacatgcag
     8521 cgtatgcggc tatcgaacgc attttaaatg aatattttag agaagaaaat ttatatcaag
     8581 tgccacctca aaatcatcaa tggtctatac aattatcaga tctcgaaacg ttaacgggtg
     8641 aatttcgcta ttggtctgcg atggggcatc atatgtatca tccagaggta tggcttattg
     8701 atggaaaaag taaaaaaata acaacttata aagaagcaat tgcgcgtatt ttgcaatata
     8761 tggctcaaag tgcagataat caaacggcag tgcaacaaca tatggcgcaa attatgtctg
     8821 acatcgataa tagcattcat cgcacggcgc gttatttgca aagtaacaca atagactacg
     8881 tagaggatcg ctatatcgtt tcagaacaat ctttatactt aggtcatcca tttcatccga
     8941 ctcctaagag tgcaagtggg ttttcagaag cagatttaga gaaatatgca cccgaatgtc
     9001 atacatcatt ccaattgcat tatttagctg tgcataaaaa tgttctgctc acgcgctatg
     9061 tagaaggtaa agaagatcag gttgagaaag tgttgtatca attagcagat ataaatatat
     9121 cagagatacc taaagatttt attttattac caatacatcc ttatcaaatc aatgtgttgc
     9181 gacagcatcc acagtatatg caatatagtg aacaaggttt aataaaagac cttggcgttt
     9241 ccggtgattt agtgtacccg acgtcttcgg ttagaactgt attttcaaaa gcattaaaca
     9301 tttatttaaa attaccgata cacgttaaaa ttactaattt tatacgtacg aatgaccttg
     9361 aacagattga acggacaatt gatgccgcgc aagttatcgc atcagtcaaa gatgaggttg
     9421 aaacacccca ttttaaattg atgtttgaag aaggatatcg tgcattgtta ccgaatccat
     9481 tagggcaaac agttgaacct gaaatggatt tattaacaaa tagtgccatg attgttcgtg
     9541 aagggatacc gaattaccat gctgataaag atattcatgt attggcgtca ttatttgaaa
     9601 cgatgcctga ttcatcgatg tctaagttat cacaagtgat tgagcaaagt ggtttagcac
     9661 cagaaccatg gcttgaatgt tatttggatc gtacattatt gccgatatta aagctgttta
     9721 gtaacacagg cattagtcta gaagcacatg tacaaaatac attaattgaa ttaaaagatg
     9781 gcatacccga cgtatgcttt gtcagagatc ttgaaggcat ttgtctatct agaacgattg
     9841 ctactgaaaa acaacttgtg ccaaatgttg tggcagcatc aagccctgtt gtatatgcac
     9901 acgatgaagc atggcatcgt cttaaatatt atgttgtagt aaatcactta ggacatttag
     9961 tatcaactat tggtaaagcg actagaaatg aagttgtgtt atggcaactt gtagcgcatc
    10021 gtcttatgac atggaaaaaa gaatacgcga ataacgcagt atttgttgac tgtgtagaag
    10081 atttatatca aacgccgacc attgcggcta aagcgaattt gatgagtaaa ttgaatgatt
    10141 gtggtgcaaa ccctatttat acacatatac caaatccaat ttgtcataac aaggaggtat
    10201 cgtattgtga atcaaacaat tcttaatcgt gtaaagacta gagtgatgca ccaactggta
    10261 tcatcactta tttatgagaa tattgttgtg tataaagcgt catatcaaga cggtgtcggt
    10321 cattttacaa tagaaggaca tgattcagag tatcgtttta ctgctgaaaa gacacatagc
    10381 tttgatcgta tacgtatcac atcaccaatt gagcgtgtcg taggagatga ggcagataca
    10441 acaacagact atacacaatt attgagagaa gctgtattta catttcctaa aaatgatgaa
    10501 aagctagaac aatttattgt tgagttatta cagacagaat taaaagatac acaaagtatg
    10561 cagtatcgag aatcaaaccc accagcaaca cctgagacat ttaacgacta tgaattttat
    10621 gcgatggaag ggcatcagta tcatccaagt tacaaatcac gtttaggatt tacgttgagt
    10681 gataatttga aatttggacc tgattttgta ccaaacatta aactgcagtg gttagctatc
    10741 gacaaagata aagtagaaac gacggtatca agaaatgttg tagttaaaga aatgttacgt
    10801 caacaagttg gtgataagac ttatgaacat tttgtacagc aaattgaagc gtctggcaaa
    10861 catgtaaatg atgttgagat gatacctgta cacccatggc agtttgaaca tgtcatccaa
    10921 gttgatttgg ctgaagaaag gcttaatggc acagtactat ggttagggga aagtgatgag
    10981 ctatatcacc ctcaacaatc gattcgtacg atgtcgccaa tagacacgac aaaatattat
    11041 ttaaaggtac caataagtat aacgaatact tcaacgaaac gagtgttggc gcctcataca
    11101 attgaaaatg cagcgcaaat tacggattgg ttaaagcaga tacagcaaca agatacgtat
    11161 ttaaaagatg aattaaagac agcttttcta ggggaagtct taggacagtc ttatttaaat
    11221 acacaacttt cgccttataa acaaactcaa gtttatggtg cgttaggtgt tatatggcgt
    11281 gaaaatatat atcatatgtt aatcgatgaa gaggatgcga taccatttaa tgcactttat
    11341 gcgagtgata aggatggtgt accattcatt gaaaagtgga ttaaacaata tggttctgaa
    11401 gcttggacaa agcaattttt agctgtagcg attcgtccaa tgattcatat gctttattat
    11461 cacggtattg cctttgaatc gcatgcacaa aatatgatgc tcattcatga aaatggttgg
    11521 cctacacgta ttgccttaaa agattttcat gatggtgttc gttttaagcg tgagcattta
    11581 agtgaagctg cttcacacct gacattaaag ccaatgccag aagcacataa aaaagtgaat
    11641 agtaattcat ttattgaaac agatgacgaa cgtttagtac gcgacttttt acatgatgca
    11701 tttttcttta ttaatatcgc cgaaatcatc ttatttattg aaaagcaata tggtatcgat
    11761 gagcagcgac aatggaaatg ggttaaagac attatcgagg cgtatcaaga agcatttcca
    11821 gagttgaata actatcaaca tttcgatttg tttgaaccta cgattcaagt tgaaaagtta
    11881 acgacacgtc gattattaag tgactccgag ttaagaattc atcatgttac aaatccatta
    11941 ggtgtaggag gtataaatga tgcaacaact atctctgaaa catagattaa acaatggtga
    12001 ttcagtttat ggcattttta attctatacc ggacccattg atgatcgagg ttatcgcagc
    12061 aagcgggtat gactttgttg tgattgatac agaacacgtg gcgattaatg atgagacact
    12121 agcgcattta attcgtgcag ctgaagcagc gcatattata ccaattgtac gtgtcactgc
    12181 agtgatagat agagatatca ttaaagtgtt ggatatgggt gcgagaggta ttattgtgcc
    12241 acacgttaaa gatcgtgaga cagttgagca tattgtgaaa ttaagtcgtt attacccgca
    12301 aggattaaga agtttgaatg gtggtcgcat ggcaagattt ggacgtacac cattacttga
    12361 tgcaatggag atggctaatg agcatattat ggtgattgcc atgatagaag atgttgaagg
    12421 ggttatggcc attgacgata tagctcaagt cgaaggttta gacatgatag tcgaaggtgc
    12481 cgcagattta tcccagtcac ttggcatacc atggcaaacg cgtgatgatc aagtaacatc
    12541 acatgttcaa catatttttg aaattgtgaa tgcacatggt aaacattttt gtgcattacc
    12601 acgtgaagat gaagatattg caaaatggca ggcacaaggt gtacaaacat ttattttagg
    12661 tgatgatcgc ggaaaaatat atcgccattt aagtgcatct ctagcgacgt ctaaacagaa
    12721 aggggatgaa ggctaatgcg tatagttcaa cctgttattg aacaattaaa agcacaatct
    12781 catccagttt gtcattatat ctatgattta gtcggactgg aacatcattt gcaacatatt
    12841 acatcgtcat tgccgagtaa ttgtcaaatg tactatgcaa tgaaagcaaa tagtgaacga
    12901 acaatcctag atacaattag tcagtatgtt gaaggattcg aagttgcatc tcaaggtgaa
    12961 atagcaaaag gtcttgcttt taaaccagca aatcatatta tttttggtgg ccctggtaag
    13021 acagacgagg aactaagata tgcagtaagt gaaggtgttc agcgtattca tgttgaaagt
    13081 atgcatgaat tacaaaggct aaatgccatc ttagaagatg aagataagac acaacacatt
    13141 ttattgcgtg ttaatttagc aggaccattt cccaatgcaa cgttgcatat ggcaggacgc
    13201 cctacacaat ttggtatttc tgaagacgaa gttgatgatg tcattgaagc tgcgctcgca
    13261 atgccaaata ttcatctaga tggatttcat tttcattcta tttctaacaa tttagactcg
    13321 aatttacatg tcgatgtagt gaaactttat tttaaaaaag caaaggcatg gtctgaaaaa
    13381 catcgatttc cactcaaaca tatcaatctt ggtggtggta taggggttaa ctatgcagat
    13441 ttaactagcc aatttgagtg ggataatttt gtagaacgtt ttaaaacact tatcgttgag
    13501 caagaaatgg aagatgtgac attgaacttt gaatgtgggc gctttattgt ggcacatatt
    13561 ggttactatg tgacagaagt gctagatatt aagaaagtgc atggtgcttg gtatgccatt
    13621 ttaagaggag gtacgcaaca atttagactg ccggtatctt ggcagcataa ccatcctttt
    13681 gaaatttatc gctataagga caatccatat tcatttgaaa aagtttcaat ttcgagacag
    13741 gacacaacgt tagtcggtca attatgtaca ccgaaagatg tctttgctag agaagtaaag
    13801 atagacgcaa tcagtacagg cgacgttatt gttttcaaat atgcaggtgc atacggatgg
    13861 tctatatcac atcacgattt cttaagccat ccacatcctg aatttattta tttaacgcaa
    13921 acaaaggagg atgaataact attgaatcat attcatgaac atttaaaatt ggtaccagta
    13981 gataaggttg atcttcacga aacattcgaa cctttaagat tggaaaagac gaaaagtagt
    14041 attgaagcag atgattttat acgtcatcct attttagtga cagcgatgca acatggtaga
    14101 tatatggtta tagatggtgt gcatcggtat acaagtttga aagcgttagg atgtaagaaa
    14161 gttccagtgc aagaaatcca tgaaacacaa tattcaatta gtacgtggca acataaagtt
    14221 ccatttggtg tgtggtggga aacgttacaa caagaacatc gcttgccatg gactactgag
    14281 acaagacaag aagcgccatt tattacgatg tgtcatggtg atacagaaca atatttgtat
    14341 acaaaagatt taggcgaagc acattttcaa gtatgggaaa aggttgtcgc aagttatagt
    14401 ggttgttgtt ctgtagagag aattgcacaa ggtacatatc cttgtctttc tcaacaagat
    14461 gtactcatga agtatcagcc attgagttat aaggaaattg aagcggttgt tcataaaggg
    14521 gaaactgtgc cagcaggtgt gacacgcttt aatatttcag gacgatgtct taatcttcaa
    14581 gtaccactgg cattgcttaa acaagatgat gatgttgaac aactgcgcaa ttggaagcag
    14641 tttttagcag ataagtttgc caatatgaga tgctatactg aaaaagtata cttggtggag
    14701 caatagtttt actgtgatgt tgagggaaat atgatgattt agcgtattga tagcgaaaat
    14761 ataataaaac aatatagtgt ggagaacttt tgatatttta taaatattgg agttctccat
    14821 ttttgtattt tgcatataaa aattaaataa aataaggtat attaaggtaa agtataaatt
    14881 ttaaataaat ggggaatgag catgagctca attataggaa gaataacaat ttggataggc
    14941 atcgtagctc aaatatattt tagtgtcgtt tttgttagga tgatatctat taatattgct
    15001 ggaggctctg attacgaaac aattttttta ttaggattaa tattgactct tttcactgtt
    15061 ttaccaacca tctttactgc gatttatatg gaaagttact ctgtaatcgg aggtgcactt
    15121 tttattgttt atgcgattat tgcactgtgt ttatataatt tcctttcggc aattttatgg
    15181 ctgattggtg gtattttgct gatttggaat aaatactcaa aagatgaatc gacagacgaa
    15241 aatgaaaaag ttgatattga aagtacagag aatcaatttg aatctaaaga taaaatcact
    15301 aaagaataaa gagaatattt aaggcggagt ataaatatta aataaatggg gaatagacat
    15361 ggaaaaaaat gtagaaaaat cattcataaa gataggttta tattttcaaa tagcttatat
    15421 agtactcatg gctataactt tatgtgggtt tgtaatttgc tatggactaa ttttcggcct
    15481 tttctattta ttatcaggta gcagagctga ttatttaata gtaacaatag ttatatcggc
    15541 aataatttct atatttgtaa ttatactttc aatcgtacct gtcatcgtat tggcatctga
    15601 cttatttaaa gaaaggattt caaaaggtgt catattaatt gtattggcta ttatcgcttt
    15661 agtattatgc aactttgtat ctgcaatact ctggtttgtt tcagccatat ctattttagg
    15721 tagaaaaaaa ttagtagctg cagcagatac taccactatt caaaaaagta aagggaacgc
    15781 aaatcaagca tcacataaag acacgggtaa aaaggaactt gatagtcaag acatgatgga
    15841 acatcctgag tttaaaaatc ccacgactaa aaacctagaa ggattaaacg aagaaataca
    15901 taaagatgaa gctacaacta aagttgacag tgataacacg caaccgccga ttgaatcaaa
    15961 agaccatgtc tcgaaaaaag attgatgaca aactaatcga gagacttaaa aaataatatt
    16021 caacataagc acttttaaag cgacatttaa acgcattgcc aatcactaat ggtagtgcgt
    16081 ttaactatac cttaaatatc tgaatatttt gttaaatgga gttatctttg ttgtactatt
    16141 caaatgaaga ggagtaaaat gtaattaaag gaaagtaatt tgaggagtga tctttatgac
    16201 aaacaacaaa gtagcgttag taactggcgg tgcacaaggg attggtttta aaattgcaga
    16261 acgtttagtg gaagatggtt tcaaagtagc agttgttgat tttaatgaag aaggggcaaa
    16321 agcagctgca cttaaattat caagtgatgg tacaaaagct attgctatca aagcagatgt
    16381 atcaaaccgt gatgatgtat ttaacgcagt aagacaaact gcagcgcaat ttggtgattt
    16441 ccatgtcatg gttaacaatg ccggccttgg accaacaaca ccaatcgata caattactga
    16501 agaacagttt aaaacagtat atggcgtgaa cgttgcaggt gtgctatggg gtattcaagc
    16561 cgcacatgaa caatttaaaa aattcaatca tggtggtaaa attatcaatg caacatctca
    16621 agcaggcgtt gagggtagcc caggattgtc tttatattgc agtacaaaat tcgcagtgcg
    16681 aggtttaaca caagtagccg cacaagattt agcgtctgaa ggtattactg tgaatgcatt
    16741 cgcacctggt atcgttcaaa caccaatgat ggaaagtatc gcagtggcaa cagccgaaga
    16801 agcaggtaaa cctgaagcat ggggctggga acaatttaca agtcagattg ctttgggcag
    16861 agtttctcaa ccagaagatg tttcaaatgt agtgagcttc ttagccggta aagactctga
    16921 ttacattact ggacaaacaa ttattgtaga tggtggtatg agattccgtt aataatcatc
    16981 cactaatgat aaataaatcc ttattgttaa gtttaatcac ttagcagtaa ggatttttta
    17041 gtgcacttag aagggattgt attggtagaa aattaataag cgaagttctt aagtgagtta
    17101 tgatgtcaca gtctaatgca tcagttgaaa gcattactag tattaacaca cccaagatat
    17161 tataaaacaa cacaaaaacg ccactatcta atttatctca ataaaaattc acaaagttat
    17221 ctcattttat ttttataaat aaaaaatatc gataaaaagc ttacaatact ttatgttttt
    17281 atgatatatt tttaatgtat aaatgaggtg gaagatttgg aaagagtttt gataactggt
    17341 ggggctggtt ttattgggtc gcatttagta gatgatttac aacaagatta tgatgtttat
    17401 gttctagata actatagaac aggtaaacga gaaaatatta aaaatttggc tgacgatcat
    17461 gtgtttgaat tagatattcg tgaatatgat gcagttgaac aaatcatgaa gacatatcaa
    17521 tttgattatg ttattcattt agcagcatta gttagtgttg ctgagtcggt tgagaaacct
    17581 atcttatctc aagaaataaa cgttgtagca acattaagat tgttagaaat cattaaaaaa
    17641 tataatagtc atataaaacg ttttgtcttt gcttcgtcag cagctgttta tggtgatctt
    17701 cctgatttac ctaaaagtga tcaatcatta atcctaccat tatcaccata tgcaatagat
    17761 aaatattacg gcgaacggac gacattaaat tattgctcgt tatataacat accaacagcg
    17821 gttgttaaat tttttaatgt atttgggcca agacaggatc ctaagtcaca atattcaggt
    17881 gtgatttcaa agatgttcga ttcatttgag cataacaagc catttacatt ttttggtgac
    17941 ggactgcaaa ctagagattt tgtatatgta tatgatgttg ttcaatctgt acgcttaatt
    18001 atggaacaca aagatgcaat tggacacggt tataacattg gtacaggcac ttttactaat
    18061 ttattagagg tttatcgtat tattggtgaa ttatatggaa aatcagtcga gcatgaattt
    18121 aaagaagcac gaaaaggaga tattaagcat tcttatgcag atatttctaa cttaaaggca
    18181 ttaggatttg ttcctaaata tacagtagaa acaggtttaa aggattactt taattttgag
    18241 gtagataata ttgaagaagt tacagctaaa gaagtggaaa tgtcaaaaaa ataacattga
    18301 agctgtccat aataataagg gttatgccta tcaaagaaaa ttagacaaac tagaagaagt
    18361 gagaaaaaga tattacccaa ttaaacgtgc gattgactta attttaagca ttgttttatt
    18421 atttttaact ttaccgatta tggttatatt cgccattgct atcgtcatag actcaccagg
    18481 aaaccctatt tatagtcagg ttagagttgg gaagatgggt aaattaatta aaatatacaa
    18541 attacgttcg atgtgtaaaa acgcagagaa aaatggtgcg caatgggctg ataaagatga
    18601 tgatcgtata acaaatgtcg ggaagtatat tcgtaaaaca cgcattgatg aattaccaca
    18661 actaattaat gttgttaaag gggaaatgag ttttattgga ccacgcccgg aacgtccgga
    18721 atttgtagaa ttatttagtt cagaagtgat aggtttcgag caaagatgtc ttgttacacc
    18781 agggttaaca ggacttgcgc aaattcaagg tggatatgac ttaacaccgc aacaaaaact
    18841 gaaatatgac atgaaatata tacataaagg tagtttaatg atggaactat atatatcaat
    18901 tagaacattg atggttgtta ttacagggga aggctcaagg tagtcttaat ttacttaata
    18961 agttcaaata aaagttacat tttaaagatt gtgaccaatt gttacagtat aacgaggaat
    19021 cccttgagac agtatcaaat ggcattaaga aatatgtgcc atcattgatt tgcgtggcta
    19081 taaatactat tcatctgatg agatagccat gttaagaaat tgaaagtata gcattaaagg
    19141 ggtttgtaac agttgaaaat tatatattgt attactaaag cagacaatgg tggtgcacaa
    19201 acacatctca ttcaactcgc caaccatttt tgcgtacacc atgatgttta tgtcattgta
    19261 ggcaatcatg gaccaatgat tgaacaacta gatgcaagag ttaatgtaat tattctcgaa
    19321 catttagtag gtccaattga ctttaaacaa gatattttag ctgtcaaagt gttagcacag
    19381 ttattctcga aaattaaacc tgatgttatc catttacatt cttccaaagc tggaacggtc
    19441 ggacgaattg cgaagttcat ttcgaaatcg aaagacacac gtgtagtttt tactgcacat
    19501 ggatgggctt ttacagaggg tgttaaacca gctaaaaaat ttctatattt agttatcgaa
    19561 aaattaatgt cacgtattac agatagcatt atttgtgttt cagatttcga taaacagtta
    19621 gcgttaaaat ctcgctttaa tcgattgaaa ttaaccacaa tacataatgg tattgcagat
    19681 gttcccgctg ttaagcaaac gctaaaaagc caatcacata acaatattgg cgaagtagtt
    19741 ggaatgttgc ctaataagca agatttacag attaatgccc cgacaaagca tcaatttgtt
    19801 atgattgcaa gatttgctta tccaaaattg ccacaaaatc taatcgcggc aatagagata
    19861 ttgaaattac ataacagtaa tcatgcgcat tttacattta taggcgatgg acctacatta
    19921 aatgattgtc agcaacaagt tgtacaagct gggttagaaa atgatgtcac atttttgggc
    19981 aatgtcatta atgcgagtca tttattatca caatacgata cgtttatttt aataagtaag
    20041 catgaaggtt tgccaattag cattatcgaa gctatggcta caggtttgcc tgttatagcc
    20101 agtcatgttg gcggtatttc agaattagta actgataatg gtatatgtat gatgaacaac
    20161 caacccgaaa ctattgccaa agtcctggaa aaatatttaa tagacagtga ttacatcaaa
    20221 atgagtaatc aatctagaaa acgttattta gaatgtttta ctgaggagaa aatgattaaa
    20281 gaagtggaag acgtttataa tggaaaatca acacaatagt aaatatctaa cattgttact
    20341 tatcggttta gcggttttta ttcagcaatc ttcggttgtt gctggtgtga atgtatctat
    20401 agctgacttt attacattac taatattagt ttatttactg ttttttgcta accatttatt
    20461 aaaggcaaat cattttttac agtttttcat tattttgtat acataccgta tgattattac
    20521 gttgtgttta ctattttttg atgatttgat atttattacg gttaaggaag ttcttgcatc
    20581 tacagttaaa tatgcatttg tagtcattta tttctattta gggatgatca tctttaagtt
    20641 aggtaatagc aaaaaagtaa tcgttacctc ttatattata agcagtgtga ctataggtct
    20701 attttgtatt atagctggtt tgaacaagtc ccctttacta atgaaattgt tatattttga
    20761 tgaaatacgt tcaaaaggat taatgaatga ccctaactat ttcgcgatga cacagattat
    20821 tacattggta cttgcttaca agtatattca taattacata ttcaaggtcc ttgcatgtgg
    20881 catcctgcta tggtctttaa ctacaacggg atctaagact gcgtttatca tattaatcgt
    20941 cttagccatt tatttcttta ttaaaaagtt atttagcaga aatgcggtaa gtgttgtgag
    21001 tatgttagtg attatgctga tattactttg ttttaccttt tataatatca actactattt
    21061 attccaatta agcgaccttg atgccttacc gtcattagat cgaatggcgt ctatttttga
    21121 agaggggttt gcatcattaa atgatagtgg atctgagcga agtgttgtat ggataaatgc
    21181 catttcagta attaaatata cattaggttt tggtgttggc ttagtggatt atgtacatat
    21241 tggctcgcaa attaatggta ttttacttgt tgcccataat acatatttgc aaatttttgc
    21301 ggaatggggc attttattcg gtgcgttatt tatcatattt atgctttatt tactgtttga
    21361 attatttaga tttaacattt ctggtaaaaa tgtaacagca attgttgtaa tgttgacgat
    21421 gctgatttac tttttaacag tatcgtttaa taactcaaga tatgtcgctt ttattttagg
    21481 aattatcgtc tttattgttc aatatgaaaa gatggaaagg gatcgtaatg aagagtgatt
    21541 cactaaaaga aaatattatt tatcaagggc tataccaatt gattagaacg atgacaccac
    21601 tgattacaat acccattatt tcacgtgcat ttggtcccag tggtgtgggt attgtttcat
    21661 tttctttcaa tatcgtgcaa tactttttga tgattgcaag tgttggcgtt cagttatatt
    21721 ttaatagagt tatcgccaag tccgttaacg acaaacggca attgtctcag cagttttggg
    21781 atatctttgt tagtaaatta ttattagcgt taacagtttt tgcgatgtat atcgctgtga
    21841 ttacaatctt tatagatgat tactatctta ttttcctact acaaggtatc tatattatag
    21901 gtgcagcact cgatatttca tggttttatg ctggaactga aaagtttaaa atacctagcc
    21961 ttagtaatat tgttgcgtct ggtattgtat taagtgtcgt tgttatcttt gtcaaagatc
    22021 aatcagattt atcattgtat gtatttacta ttgctattgt gacggtatta aaccaattac
    22081 ctttgtttat ctatttaaaa cgatacatta gctttgtttc ggttaattgg atacacgtct
    22141 ggcaattgtt tcgttcgtca ttagcatact tattaccaaa tggacagctc aacttatata
    22201 ctagtatttc ttgcgttgtt cttggtttag taggcacata ccaacaagtt ggtatctttt
    22261 ctaacgcatt taatatttta acggtcgcaa tcataatgat taatacattt gatcttgtaa
    22321 tgattccgcg tattactaaa atgtctttac atcaatcaca tagtttaact aagacgttag
    22381 cagataatat gaacattcaa ttgatattaa caatacctat ggtctttggt ttgattgcaa
    22441 ttatgccatc attttattta tggttctttg gtgaggaatt cgcatcaact gtcccattga
    22501 tgaccatttt agcgatactt gtattaatca ttcctttaaa tatgttgata agcaggcaat
    22561 atttattaat agtgaataaa ataagattat ataatgcgtc aatcactatt ggtgcggtaa
    22621 tgaatttagt attatgtctt gttttgatat atttttatgg aatttacggt gctgctattg
    22681 cgcgtttaat tacagagttt ttcttgctca tttggcgatt tattgatatt actaaaatca
    22741 atgtgaagtt gaatattgta agtacgattc aatgtgtcat tgctgccgtt atgatgttta
    22801 ttgtgcttgg tgtggtcaat cattatttgc ctcctacaat gtacgctacg ctgctattaa
    22861 ttgctattgg tatagtagtt tatcttttat taatgatgac tatgaaaaat caatacgtat
    22921 ggcaaatatt gaggcatctt cgacataaaa caatttaagt atcggtaacg ctatacttta
    22981 ggaatttaag attaagaaga aaaggcaatt tcttattgaa aaatggaagt tgtctttttt
    23041 aattcttttt aaaagcggga aacaaaagca gttaaatgcc tttttgcatt caatattaaa
    23101 tattatatca attccgaat
//
LOCUS       DAHHOJ010000034        23074 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.034, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000034 DAHHOJ010000000
VERSION     DAHHOJ010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 23074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23074
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.034"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            1..90
                     /locus_tag="NBH32_001139"
                     /pseudo
     CDS             1..90
                     /locus_tag="NBH32_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499555.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(238..555)
                     /locus_tag="NBH32_001140"
     CDS             complement(238..555)
                     /locus_tag="NBH32_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HCD1514757.1"
                     /translation="MNKLLQSLSALGVSATLVTPNLNADATTNTTPQIKGANDIVIKK
                     GQDYNLLNGISAFDKEDGDLTDKIKVDGQIDTSKSGKYQIKYHVTDSDGAIKISTRYI
                     EVK"
     gene            complement(1220..2080)
                     /gene="folD"
                     /locus_tag="NBH32_001141"
     CDS             complement(1220..2080)
                     /gene="folD"
                     /locus_tag="NBH32_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCD1514758.1"
                     /translation="MVAKILDGKQIAKDYRQGLQDQVEALKEKGFTPKLSVILVGNDG
                     ASQSYVRSKKKAAEKIGMISEIVHLEETATEEEVLNELNRLNNDDSVSGILVQVPLPK
                     QVSEQKILEAINPDKDVDGFHPINIGKLYIDEQTFVPCTPLGIMEILKHADIDLEGKN
                     AVVIGRSHIVGQPVSKLLLQKNASVTILHSRSKDMASYLKDADVIVSAVGKPGLVTKD
                     VVKEGAVIIDVGNTPDENGKLKGDVDYDAVKEIAGAITPVPGGVGPLTITMVLNNTLL
                     AEKMRRGIDS"
     gene            2281..2763
                     /gene="purE"
                     /locus_tag="NBH32_001142"
     CDS             2281..2763
                     /gene="purE"
                     /locus_tag="NBH32_001142"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCD1514759.1"
                     /translation="MKVAVIMGSSSDWKIMQESCNMLDYFEIPYEKQVVSAHRTPKMM
                     VQFASEARESGIDIIIAGAGGAAHLPGMVASLTTLPVIGVPIETKSLKGIDSLLSIVQ
                     MPGGIPVATTAIGAAGAKNAGILAARMLSIQNPSLVEKLNHYESSLIQKVEDMQNELQ
                     "
     gene            2750..3874
                     /gene="purK"
                     /locus_tag="NBH32_001143"
     CDS             2750..3874
                     /gene="purK"
                     /locus_tag="NBH32_001143"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCD1514760.1"
                     /translation="MNFNKLKFGATIGIIGGGQLGKMMAQSAQKMGYKVAVLDPAEDC
                     PCRYVAHEFIQAKYDDEKALNQLGQKCDVITYEFENISAQQLKLLCEKYNIPQGYQAI
                     QLLQDRLTEKETLKSAGTKVVPFISVKESTDIDKAIETLGYPFIVKTRFGGYDGKGQV
                     LINNEKDLQEGFKLIETSECVAEKYLNIKKEVSLTVTRGNNNQITYFPLQENEHRNQI
                     LFKTIVPARIDKTAEAKEQVNKIIQSIHFIGTFTVEFFIDSNNQLYVNEIAPRPHNSG
                     HYSIEACDYSQFDTHILAVTGQSLPNSIELLKPAVMMNLLGKDLDLLENEFNEHPEWH
                     LHIYGKSERKDSRKMGHMTVLTNDVNQTEQDMYAKFEGSN"
     gene            3878..4582
                     /locus_tag="NBH32_001144"
     CDS             3878..4582
                     /locus_tag="NBH32_001144"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCD1514761.1"
                     /translation="MTLLYEGKAKRIFSTNQENELRVEYKDEVTAGNGAKKDTMAGKG
                     RLNNQITSIIFKYLQENGIESHFIKQLSETDQLVKPVKIIPLEVVVRNIASGSITKRL
                     GFENGEVFREPLVEFFYKNDALNDPLITDDHIKLLNIAIDKDIETLKTKALEINQVLK
                     QLMDAMNLKLVDFKIEFGKTETGQILLADEISPDTCRIWDKATNANFDKDVYRNNTGS
                     LIETYQIFLNKLEDLK"
     gene            4582..4845
                     /gene="purS"
                     /locus_tag="NBH32_001145"
     CDS             4582..4845
                     /gene="purS"
                     /locus_tag="NBH32_001145"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HCD1514762.1"
                     /translation="MKTIELHITLQPQVLDTQGQTLTRAVHDLGYAQVNDIRVGKVLY
                     MTVDEVSDEKVHNIITTLSEKLFANTVIEEYSYKVLDDEKENA"
     gene            4847..5518
                     /gene="purQ"
                     /locus_tag="NBH32_001146"
     CDS             4847..5518
                     /gene="purQ"
                     /locus_tag="NBH32_001146"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase I"
                     /protein_id="HCD1514763.1"
                     /translation="MKFAVLVFPGSNCDRDMFNAAIKSGVEAEYVDYRETSLSGFDGV
                     LIPGGFSFGDYLRSGAMASVAPIISEVKRLAAEGKPVLGVCNGFQILTEIGLLPGALL
                     HNDSHLFISRNEKLEIVNNQTAFTNLYEQGEKVIYPVAHGEGHYYCTDEIYQQLKANN
                     QIILKYVNNPNGSYDDIAGIVNEKGNVCGMMPHPERALETLLGTDSGVKLFEAMVKSW
                     REQHV"
     gene            5511..7700
                     /gene="purL"
                     /locus_tag="NBH32_001147"
     CDS             5511..7700
                     /gene="purL"
                     /locus_tag="NBH32_001147"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HCD1514764.1"
                     /translation="MSKFIEPSVEEIKLEKVYQDMGLSDQEYEKVCDILGRQPNFTET
                     GIFSVMWSEHCSYKHSKPFLKQFPTSGEHVLMGPGEGAGVVDIGDNQAVVFKVESHNH
                     PSAIEPYQGAATGVGGIIRDIVSIGARPINLLNSLRFGELDNKQNQRLLKGVVKGIGG
                     YGNCIGIPTTAGEIEFDERYDGNPLVNAMCVGVINHDMIQKGTAKGVGNSVIYVGLKT
                     GRDGIHGATFASEELTEESESKRPSVQIGDPFVGKKLMEATLEAITFDELVGIQDMGA
                     AGLTSSSSEMAAKGGSGLHLRLEQVPTREPGISPYEMMLSETQERMLLVVEKGTEQKF
                     LDLFDKHELDSAVIGEVTDTNRFVLTYDDEVYADIPVEPLADEAPVYILEGEEKDYNT
                     SKNDYTHIDVKDTFFKLLKHPTIASKHYLYDQYDQQVGANTIIKPGLQASVVRVEGTN
                     KAIASTIDGEARYVYNNPYEGGKMVVAEAYRNLIAVGATPLAMTDCLNYGSPEKKEIY
                     QQLIDSTKGMAEACDILKTPVVSGNVSLYNETKGTSIFPTPVVGMVGLIENVNYLNDF
                     EPQVGDKLYLIGDTKDDFGGSQLEKLIYGKVNHEFESLDLSSEVEKGESIKTAIREGL
                     LSHVQTVGKGGLLITLAKLSAHYGLGLKSSIDITNAQLFSETQGRYVVSVKSGKTLNI
                     DNAIEIGLLTDSDNFKVTTPYTEISENVSDIKQIWEGAIAQCLTTQD"
     gene            7679..9163
                     /locus_tag="NBH32_001148"
     CDS             7679..9163
                     /locus_tag="NBH32_001148"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCD1514765.1"
                     /translation="MFNYSGLNEECGVFGIWNHPEAAQLTYMGLHSLQHRGQEGAGIV
                     VSDQNELKGERGLGLLTEAIKDDQMERLKGYQHAIGHVRYATSGNKGIENIQPFLYHF
                     YDMSVGICHNGNLINAKSLRQNLEKQGAIFHSSSDTEVIMHLIRRSKAPTFEEALKES
                     LRKIKGGFTFAILTKDALYGAVDPNAIRPLVVGKMKDGTYILASETCAIDVLGAEFVQ
                     DIHAGEYVVINDKGITVKSYTHHTTTAISAMEYIYFARPDSTIAGKNVHAVRKASGKK
                     LAQESPVNADMVIGVPNSSLSAASGYAEEIGLPYEMGLVKNQYVARTFIQPTQELREQ
                     GVRVKLSAVKDIVDGKNIILVDDSIVRGTTIRRIVKMLKDSGANKVHVRIASPEFMFP
                     SFYGIDVSTTAELISASKSPEEIKDYIGADSLAYLSVDGLIESIGLDYDAPYSGLCVE
                     SFTGDYPAGLYDYEANYKAHLSHRQKQYISKNKHFFDSEGNLNV"
     gene            9156..10184
                     /locus_tag="NBH32_001149"
     CDS             9156..10184
                     /locus_tag="NBH32_001149"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCD1514766.1"
                     /translation="MSKAYEQSGVNIHAGYEAVERMSSHVKRTMRKEVIGGLGGFGAT
                     FDLSQLNMTAPVLVSGTDGVGTKLKLAIDYGKHDSIGIDAVAMCVNDILTTGAEPLYF
                     LDYIATNKVVPEVIEQIVKGISDACVETNTALIGGETAEMGEMYHEGEYDVAGFAVGA
                     VEKDDYVDGSEVKEGQVVIGLASSGIHSNGYSLVRKLINESGIDLASNFDNRPFIDVF
                     LEPTKLYVKPVLALKKEVSIKAMNHITGGGFYENIPRALPAGYAARIDTTSFPTPKIF
                     DWLQQQGNIDTNEMYNIFNMGIGYTVIVDEKDASRALKILAEQNVEAYQIGHIVKNES
                     TAIELLGV"
     gene            10187..10753
                     /gene="purN"
                     /locus_tag="NBH32_001150"
     CDS             10187..10753
                     /gene="purN"
                     /locus_tag="NBH32_001150"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCD1514767.1"
                     /translation="MVKIAIFASGSGSNFENIVEHVESGKLENIEVTALYTDHQNAFC
                     IDRAKKHGIPVYINEPKQFDSKAAYEQHLVTLLNKDKVEWIILAGYMRLIGPDLLASF
                     AGKILNIHPSLLPKYKGIDAIGQAYHSGDTITGSTVHYVDSGMDTGEIIEQRKCDIRP
                     DDSKEQLEEKVKKLEYELYPSVIAKIVK"
     gene            10768..12246
                     /gene="purH"
                     /locus_tag="NBH32_001151"
     CDS             10768..12246
                     /gene="purH"
                     /locus_tag="NBH32_001151"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCD1514768.1"
                     /translation="MKKAILSVSNKTGIVEFAKALTQLNYELYSTGGTKRILDEANVP
                     VRSVSDLTHFPEIMDGRVKTLHPAVHGGILADRNKPQHLNELSEQHIDLIDMVVVNLY
                     PFQQTVANPDVTMDEAIENIDIGGPTMLRAAAKNYKHVTTIVHPADYHEVLTRLRNDS
                     LDESYRQSLMIKVFEHTAEYDEAIVRFFKGDKETLRYGENPQQSAYFVRTSNAKHTIA
                     GAKQLHGKQLSYNNIKDADATLALVKKFDTPAAVAVKHMNPCGVGIGDTIEQAFQHAY
                     EADSQSIFGGIVALNRAVTPELAEQLHSIFLEVIIAPKFTDEALDILKQKKNVRLLEI
                     DMTIDSNEEEFVSVSGGYLVQDKDNYVVPKDEMKVVTEVVPTDEQWEAMLLGWKVVPS
                     VKSNAIILSNNKQTVGIGAGQMNRVGAAKIALERAIEINDHVALVSDGFFPMGDTVEL
                     AAQHGIKAIIQPGGSIKDQDSIDMANKHGIAMVVTGTRHFKH"
     gene            12268..13515
                     /locus_tag="NBH32_001152"
     CDS             12268..13515
                     /locus_tag="NBH32_001152"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCD1514769.1"
                     /translation="MNILVIGAGGREHALAYKLNQSNLVKQVFVIPGNEAMTPIAEVH
                     TEISESNHQGILDFAKQQNVDWVVIGPEQPLIDGLADILRANGFKVFGPNKQAAQIEG
                     SKLFAKKIMEKYNIPTADYKEVERKKDALTYIENCELPVVVKKDGLAAGKGVIIADTI
                     EAARSAIEIMYGDEEEGTVVFETFLEGEEFSLMTFVNGDLAVPFDCIAQDHKRAFDHD
                     EGPNTGGMGAYCPVPHISDDVLKLTNETIAQPIAKAMLNEGYQFFGVLYIGAILTKNG
                     PKVIEFNARFGDPEAQVLLSRMESDLMQHIIDLDEGKRTEFKWKNESIVGVMLASKGY
                     PDAYEKGHKVSGFDLNENYFVSGLKKQGNTFVTSGGRVILAIGKGDNVQDAQRDAYEK
                     VSQIQSDHLFYRHDIANKALQLK"
     gene            complement(13781..14587)
                     /locus_tag="NBH32_001153"
     CDS             complement(13781..14587)
                     /locus_tag="NBH32_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCD1514770.1"
                     /translation="MYEQWKVRQSFVDDVNIITKLFLGIILFFFIIFVHNFDFMLYIT
                     ILMLVFLLMFNGTQFKITSIFITISIGFALLSSLFMILYGNGSHTILKFGFIHITTES
                     LVRGLHVSLRTIAISFFGILIALTSQVVMIFYSLMQHLKVKSKVAYAFMAAIRMIPLM
                     ISSLIQLRRSLKMRYQMIDAANYRGLKRFKHLIIPLLSQNIRKAHQLSVAMESKGFRD
                     GPRTYYYNVPFSYKDIIFIIVLIAIITLAYYCSITLPITGIDDVRLGRVG"
     gene            complement(14580..15980)
                     /locus_tag="NBH32_001154"
     CDS             complement(14580..15980)
                     /locus_tag="NBH32_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514771.1"
                     /translation="MLKVSDLRLKYPSGQRKIFDHLNITIQDKEKVLLLGPSGCGKST
                     LLNVLSGIVPNLIELPMKYDELIVDPLSGVIFQDPDSQFCMPKVYEELAFVLENRQVP
                     REDMDALIINALNMVNLNVTPETYIKDLSGGMKQKLAIVETILQQSKTLFLDEPTAML
                     DVQATEDLWTKLIELWEDQTVVIVEHKVKHIWNHVDRVILMDYNGNIIADECPEIILQ
                     KYVHLLSEYGVWHPRAWEFAPSRVDFPTTNSHLLQFKNGRIIRGKSTLLSFSDLEIGL
                     GEWITITGANGSGKTTLLESIMQLIKYQGDVYFENQRLTKIKHAAKHMYLVYQNPELQ
                     FITNSVYDEINIHFNHLSKDQSDDETIQLLKLLDLENVKDQHPYELSIGQKRRLSVAT
                     ALSSKADIIFLDEPTFGLDSHNTFQLIKLFQKRINLGQSIVMVTHDDEIIERYPSRRL
                     KISDGTLLDCDGDTNV"
     gene            complement(15995..16570)
                     /locus_tag="NBH32_001155"
     CDS             complement(15995..16570)
                     /locus_tag="NBH32_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1514772.1"
                     /translation="MSKGLKLSEILVTVLISVVFAVIYNLWWFVYNGVQATGLHLEQL
                     TNGVWFMAAIVCYLIIPKPGIALLAEFAAGAGETIIMGRFDIPTIVYAFIQGLACELV
                     FAIFKYQSRSVMVAMLAGFCTAIAAFPIDYFYGYLNEVAGWNLTLFIVFRLISGAVVA
                     GVLSYLLVKALDKTGVTKLFRPAAKEDYDNL"
     regulatory      complement(16647..16747)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(17069..17203)
                     /gene="graF"
                     /locus_tag="NBH32_001156"
     CDS             complement(17069..17203)
                     /gene="graF"
                     /locus_tag="NBH32_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance-associated protein GraF"
                     /protein_id="HCD1514773.1"
                     /translation="MSNENQNKKAAEKAKEVEEKLKDKKEVKTEDINQTKQDIQDTLN
                     "
     gene            17355..18650
                     /locus_tag="NBH32_001157"
     CDS             17355..18650
                     /locus_tag="NBH32_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514774.1"
                     /translation="MSFLRKHAEIIFSYLIGIVSLFTGLIILINLPLIKQLNGGKKVD
                     THVHNVWEFLNAFFSEIIKVMSRFIGNFPIVSAIVIIIFGILVMLIGHTLLRTIKYDY
                     DISIFFLVIGIMYFIITLILMTQVYGFFAVIFIIPFTIHIGYIVYKDELNQENVKNHF
                     MWIIVSYGISYLITQIALYGRIDANEIESIDILSVNAFFIIMWLLGQMAIWNFLFLRR
                     ALPLTKQELGEEEPELSRTSKGNVTNQTKIHLKQLQDKTTEYARKTRRSVDLDKIRAK
                     RDKFKKKVNDIIDIQEDDIPDWMRKPKWVKPMYVELFCGVVIFLFTFLEFNNRNALFV
                     YGDWKLSQTQYVIEWVTLLILLFIIIAYIATTLTFHLKGKFYYLQLFMGSILFFKLLT
                     EFINIMIHGLLLSVFITPTLLLMLLAIIISYSLQLRERP"
     gene            19075..20247
                     /locus_tag="NBH32_001158"
     CDS             19075..20247
                     /locus_tag="NBH32_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HCD1514775.1"
                     /translation="MKIATLNKGKETKYFNGYPLIEEEDIYSQDHLKEGDIFQIVTDK
                     SQYVATAYVGRQHKGLGWVLTYDKAQEINTAFFVKLFNTALAERDYYFNIDGTNAFRL
                     FNAEGDGVGGLTIDNYDGHLLIQWYSKGIYKFKYAILEAVRKVFDYKSIYEKVRFKDS
                     EYSGGFVEGDAPEFPIVIEENFTFYNVDLEDGLMTGIFLDQKEVRKKLRDQYAKERHV
                     LNLFSYTGAFSVIAASEASSTTSVDLANRSRSLTEENFGLNAIDPKSQYIYVMDTFDF
                     YKYAARHGHSYDTIVIDPPSFARNKKRTFSVQKDYDKLITGALNILSSEGTLLLCTNA
                     SVYPLKQFKNTIKKTLEESGVDYELTEVMGLPKDFKTHPHYKPSKYLKAVFVNIRH"
     gene            20301..20843
                     /locus_tag="NBH32_001159"
     CDS             20301..20843
                     /locus_tag="NBH32_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF697 domain-containing protein"
                     /protein_id="HCD1514776.1"
                     /translation="MGFKNNLTSNLTNKIGNSVFKIENVDGKGAMPTTIQELRERRER
                     AEAIVKRKSLMSSTMSVVPIPGLDFGVDLKLMKDIIEDVNKIYGLDHKQVNSLGDDVK
                     ERIMSAAAIQGSQFIGKRISSAFLKIVIRDVAKRTAAKQTKWFPVVGQAVSASISYYF
                     MNKIGKDHIQKCENVIKNVM"
     gene            20997..21263
                     /locus_tag="NBH32_001160"
     CDS             20997..21263
                     /locus_tag="NBH32_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HCD1514777.1"
                     /translation="MEQNSYVIIDETGIHARPATMLVQTASKFDSDIQLEYNGKKVNL
                     KSIMGVMSLGVGKDAEITIYADGSDESDAIQAISDVLSKEGLTK"
     gene            21266..22984
                     /gene="ptsP"
                     /locus_tag="NBH32_001161"
     CDS             21266..22984
                     /gene="ptsP"
                     /locus_tag="NBH32_001161"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCD1514778.1"
                     /translation="MSKLIKGIAASDGVAIAKAYLLVEPDLTFDKNEKVTDVEGEVAK
                     FNNAIEASKVELTKIRNNAEVQLGADKAAIFDAHLLVLDDPELIQPIQDKIKNENANA
                     ASALTDVTTQFVTIFESMDNEYMKERAADIRDVSKRVLSHILGVELPNPSMIDESVVI
                     VGNDLTPSDTAQLNKEFVQGFATNIGGRTSHSAIMSRSLEIPAIVGTKSITQEVKQGD
                     MIIVDGLNGDVIVNPTEDELIAYQDKRERYFADKKELQKLRDADTVTVDGVHAELAAN
                     IGTPNDLPGVIENGAQGIGLYRTEFLYMGRDQMPTEEEQFEAYKEVLEAMDGKRVVVR
                     TLDIGGDKELSYLNLPEEMNPFLGYRAIRLCLAQQDIFRPQLRALLRASVYGKLNIMF
                     PMVATINEFREAKAILLEEKENLKNEGHDISDDIELGIMVEIPATAALADVFAKEVDF
                     FSIGTNDLIQYTLAADRMSERVSYLYQPYNPSILRLVKQVIEASHKEGKWTGMCGEMA
                     GDETAIPLLLGLGLDEFSMSATSILKARRQINGLSKNEMTELANRAVDCATQEEVIEL
                     VNNYVK"
BASE COUNT         8096 a         3323 c         4319 g         7336 t
ORIGIN      
        1 gaatttgcca aaagtaattt atcgttaaac aatgaaaagt agaaatttgt ttgttcaaaa
       61 tcaaaatatt cttttaaaaa gagcggttaa gttttaataa tttgaaaggt agtcctttgg
      121 tatacagaaa aaatacaata tttgggttaa agttgcagat gaagttggaa taacaaataa
      181 aatggtagca aaacatgtac gtttgctacc attttatttg cagtatagtg atgagggcta
      241 tttaacctca atatacctag tggaaatttt aattgcacca tctgaatcag tgacatgata
      301 tttaatttga tatttaccag atttagatgt atcaatttgg ccatcgactt taattttatc
      361 ggttaaatct ccatcttctt tatcaaatgc acttatgccg tttagaaggt tataatcttg
      421 acctttctta ataacgatat cattagcgcc tttaatttgt ggtgtagtat tcgtcgttgc
      481 atctgcattt aaatttggtg ttactagtgt agcagaaaca ccgagggctg ataatgactg
      541 taatagttta ttcataatgt aacctccgaa tgataagtca tttctgagtt agctttacta
      601 taccttaatt tatattattt acacatcata ctatagtcct aatcttaccc tataaagaat
      661 gaaatttaaa tataaattta taaataatga ggaaataatt attattttga aaatctgcca
      721 caattgtaaa attaataatt cagggataat ttgaaatcaa atataacttt attcgacagt
      781 ttgaacataa atttttgtgt atcaaaacaa taagaaaagg gaggcgaaaa tgataaagaa
      841 ccactattga ttaattatgt agtagtactt tatcatagtc acagcttctg tgatggggcc
      901 tcgtcgactt gcattgtctg tagaaattgg aaatccaatt cctctttgtt ggggcccatc
      961 cccaacttgc acattattgt aagctgactt tccgccagct tctatgttgg ggccccggac
     1021 tataattgaa aaatgcttgt tacaagctta ttttcattca attaacaact gtctatataa
     1081 aattgttgag ccaagaacaa agtgaaatgc tcccttcaaa gccttcattt attcaatgtc
     1141 tactttgaag ggagcatttg atcgattaat gtcccagatt tttcttgtta ttcattcaca
     1201 ttacatgact aatgtgaatt tacgaatcaa tacctcgacg cattttttct gcaagcaaag
     1261 tattatttaa taccatagta attgttaatg gaccaacgcc accaggaact ggtgtaatag
     1321 ctccagcaat ttctttaacc gcatcataat caacgtcacc ttttaatttc ccattttcat
     1381 ctggcgtatt gccaacatcg ataattactg ctccttcttt gaccacatct tttgttacta
     1441 aaccaggctt accaactgca ctgacaatga catcagcatc ttttaaatat gatgccatat
     1501 cctttgaacg agaatgtaag attgttactg atgcattttt ttgaagtagt aacttagaaa
     1561 ctggttgtcc gacaatatga cttcgtccaa ttacaactgc atttttacct tctaaatcaa
     1621 tatcagcatg ttttaatatt tccatgatgc cgagcggtgt gcaaggtaca aaagtttgtt
     1681 catcgatata taatttccct atatttattg gatgaaaacc gtccacatct ttatcaggat
     1741 tgattgcttc taatattttc tgttcgctaa cttgttttgg tagtggtact tgtaccaaaa
     1801 taccacttac agaatcatca ttatttagtc tatttagttc gtttaatact tcctcttcag
     1861 tagctgtttc ttccaaatgt acgatttctg aaatcatacc aattttttca gctgctttct
     1921 tttttgatct aacataactt tgactagcgc catcattacc aactaatata acggataatt
     1981 taggtgtaaa acccttttct tttagcgctt caacttgatc ttgtaacccc tgtctgtagt
     2041 ctttggcaat ttgtttacca tctaaaattt tagcaaccat aaaaacttct cctcctattg
     2101 tcaatatcct aactttgtta gtttataaaa ctaatttaca tagcaaaagg cgtaaattcg
     2161 aacaaaaaat caaaataaag ttcgattttt gattgaaaaa gcagaaattg cttgttatgc
     2221 tatatctata atatacaact aaagtaattc acaattcaaa cttttgaaag ggtgtacaaa
     2281 gtgaaagtag cagtcattat gggcagttct tccgattgga aaattatgca agagagttgt
     2341 aacatgttgg attattttga aattccgtac gaaaaacaag tagtatcggc acatcgtacg
     2401 ccaaaaatga tggttcaatt tgcttctgaa gcgagagaaa gcggtataga cattatcatt
     2461 gcaggcgctg gcggtgcggc acatttacca ggtatggttg catcattaac gacgctacca
     2521 gttattggag tgccgattga aacaaaaagt ttaaagggta tagattcttt attatcaatt
     2581 gttcaaatgc caggaggtat tccggttgca acgactgcaa ttggtgcagc aggtgctaaa
     2641 aacgcaggta tacttgcagc aagaatgtta agtattcaaa atccttcttt agttgaaaaa
     2701 ctaaatcatt atgaatcttc gttaattcaa aaagtggagg acatgcaaaa tgaacttcaa
     2761 taaattaaag tttggtgcga ctattggcat tattggtggt ggtcagcttg gaaagatgat
     2821 ggcacaatca gctcaaaaaa tgggttataa agtggctgta ttggatcctg ctgaagattg
     2881 tccatgtaga tacgttgcac acgaatttat acaagccaag tatgacgatg aaaaggcact
     2941 caatcaatta ggacaaaaat gtgatgtgat tacttatgaa tttgaaaaca tttcagccca
     3001 acaattaaaa ctattatgtg aaaagtacaa tattccgcaa ggttaccaag ctatacagtt
     3061 attacaagac cgcttaactg aaaaagaaac attaaaaagt gctggtacca aagttgtccc
     3121 gttcatttca gtaaaagaat ctacagatat tgacaaagca attgaaacat taggatatcc
     3181 ttttattgta aaaactagat ttggtggcta cgatggcaaa ggtcaagttt taattaacaa
     3241 cgaaaaagac ttacaagaag gttttaaatt aattgaaact agtgaatgcg tagctgaaaa
     3301 atatttgaat atcaagaaag aagtatcact tactgttaca agaggaaaca acaatcaaat
     3361 tacttatttc ccattacaag aaaatgagca tagaaatcaa atacttttca aaacaattgt
     3421 tccagcgaga atagataaaa cagctgaggc gaaagagcaa gttaataaaa ttatccaatc
     3481 gattcatttc attggaacat ttacagttga attttttata gatagtaaca accaattgta
     3541 tgtgaacgag attgcaccaa ggcctcacaa ttccggacat tattcaattg aagcatgtga
     3601 ttattcacaa tttgatactc atattttagc agttaccgga caatcattac caaattcaat
     3661 tgaattatta aagcctgcag tcatgatgaa cttactaggt aaagacttag atttattgga
     3721 aaatgaattt aatgaacatc cagagtggca cttacatatt tatggtaagt ctgagcgtaa
     3781 agatagtaga aaaatggggc atatgactgt actaacgaat gatgtaaacc aaactgaaca
     3841 agatatgtac gctaaatttg aggggagtaa ttaaagcatg acattattat atgaaggaaa
     3901 agcgaagcgt attttctcaa caaatcaaga aaatgaatta agagttgaat ataaagatga
     3961 agttactgct ggaaacgggg ctaagaaaga cacaatggca ggtaaggggc gattaaataa
     4021 tcaaattact tctattatat ttaaatattt acaagaaaat ggaatagaaa gtcactttat
     4081 taaacaatta tctgaaacag atcaattagt taagcctgtg aaaataattc cattagaagt
     4141 agttgtccgt aatattgcta gtggatctat tacaaagcgt ttaggttttg aaaatggtga
     4201 agtttttaga gaaccacttg tggaattttt ctataaaaat gatgcattaa atgatccttt
     4261 gattacagat gatcacatca aattactgaa tattgcgata gataaagata ttgaaacatt
     4321 aaaaacgaaa gcgctagaaa ttaatcaagt attaaaacaa ttaatggatg cgatgaattt
     4381 aaaattagta gattttaaaa tcgaatttgg aaagactgag actggtcaaa ttttgttagc
     4441 ggatgaaata tctccagata catgtcgaat ttgggataaa gctaccaatg caaactttga
     4501 taaagatgta tatagaaata acactggatc actgattgaa acatatcaaa tatttttaaa
     4561 caaattggag gatttaaaat aatgaaaaca attgaactac atatcacatt acaaccacaa
     4621 gtattagata cgcaaggaca aacgcttact cgagctgtac atgacttagg ttatgcacaa
     4681 gtgaatgata ttcgtgtagg aaaagtatta tatatgacag tggatgaggt tagtgatgaa
     4741 aaggtacaca acattattac aactctaagt gaaaaattgt ttgcaaatac agtgattgaa
     4801 gaatatagct ataaagtgtt agatgatgaa aaggagaatg cataaaatga aatttgcggt
     4861 tcttgttttt ccaggttcga attgtgatag agacatgttt aatgctgcta ttaaaagtgg
     4921 tgttgaagcg gaatatgtag attatagaga aacatcacta agtggatttg atggcgtact
     4981 tattcctggt ggattttcat tcggggatta cttaagatct ggggcaatgg ctagtgtagc
     5041 gccgattatt tcggaagtta aacgtcttgc agctgaaggt aagccagtat taggtgtttg
     5101 taatgggttt caaattttaa ctgaaatagg cttattacct ggtgcattat tgcataacga
     5161 ttcacattta tttattagta gaaatgaaaa gttagaaata gtgaataatc aaacggcatt
     5221 tacaaatctt tatgaacaag gtgaaaaagt tatatatcct gtagctcacg gtgaaggtca
     5281 ttattattgt actgatgaaa tatatcaaca attaaaagct aacaatcaaa ttattctgaa
     5341 atatgtgaat aatccgaacg gttcatatga tgatattgca ggaattgtta atgaaaaagg
     5401 caatgtatgt ggcatgatgc cacatcctga aagagcttta gaaacgttgt taggtactga
     5461 tagtggtgtg aaattatttg aagcgatggt aaaaagttgg agggaacaac atgtctaaat
     5521 ttatcgaacc aagcgttgaa gaaattaaac ttgaaaaagt atatcaagat atgggattaa
     5581 gtgatcaaga atatgaaaaa gtttgcgata ttttaggcag acaacctaac tttacagaaa
     5641 caggtatctt ttctgttatg tggagtgaac attgctctta taaacattct aaaccgtttt
     5701 taaagcaatt tcctacgtca ggtgagcatg tgcttatggg gcctggtgaa ggtgcagggg
     5761 tagtcgatat aggtgataat caagccgtag tatttaaagt agagtctcac aatcatccat
     5821 cagcaattga accatatcaa ggggctgcta caggcgttgg tggaatcatt cgtgacattg
     5881 tctctattgg ggctagacct attaatttgt taaacagtct tagatttgga gaattagata
     5941 ataaacaaaa ccaaagatta cttaaaggtg ttgtaaaggg tatcggaggt tatggtaact
     6001 gcattggtat tccaacaact gctggtgaaa tcgaatttga tgaacgttat gatggcaatc
     6061 cacttgttaa tgcaatgtgt gttggtgtta tcaatcacga catgattcaa aaaggcacag
     6121 caaaaggtgt aggtaattcg gtcatttatg ttggtttgaa aactggtcga gatggtattc
     6181 atggtgctac ttttgcatct gaagaattga cggaagaaag cgaaagtaaa cgaccttctg
     6241 tacaaatcgg tgatccattt gtaggtaaaa aattaatgga agcaacactt gaagcaatta
     6301 catttgatga attagttggt attcaagata tgggtgctgc tggtttaaca tcttcatcgt
     6361 ctgaaatggc ggcaaaaggt ggtagtgggt tacatttgag attagaacaa gtgccaacac
     6421 gtgagccagg tatttctcct tatgaaatga tgctttcaga aactcaagaa cgtatgttac
     6481 tagttgttga aaaaggtact gaacaaaaat tcttagattt atttgataag cacgaattgg
     6541 atagtgctgt tataggtgaa gttacagata caaatcgttt tgttttaaca tatgatgacg
     6601 aagtttatgc tgacattcca gttgaaccac tagctgatga agcacctgta tatattttag
     6661 aaggagaaga aaaagattat aatacttcta aaaatgatta tacacacatc gatgttaaag
     6721 atactttctt taaattactt aagcatccga ctatagcatc taaacactat ttatatgatc
     6781 aatacgacca acaagttggt gccaatacga taattaagcc aggacttcaa gcatcggtag
     6841 tacgtgtgga aggcacaaat aaggcgattg cttcaacaat tgatggtgaa gcgcgttatg
     6901 tatataacaa tccatatgaa ggtggaaaga tggtagtagc tgaagcttat cgaaatttaa
     6961 ttgccgtggg tgcaacacca ttagcaatga cagattgttt aaattatggt tctcctgaaa
     7021 agaaagaaat ctatcaacag ttgatagatt caacgaaagg tatggcagaa gcatgcgaca
     7081 ttcttaagac accagtagtt tctggaaatg tatctttata taacgaaacg aaaggtactt
     7141 ctattttccc aacaccagtt gttggaatgg taggtttgat tgaaaatgta aattatttaa
     7201 atgattttga acctcaagtt ggagataaat tatatttaat cggtgatact aaggacgact
     7261 ttggtggtag tcaacttgaa aagttaattt atggcaaagt taatcatgaa tttgagtcat
     7321 tagatttgag ttcagaagtt gaaaaaggtg aatcaatcaa gaccgctatt cgtgaaggac
     7381 tattatcaca tgttcaaaca gttggtaaag gtggcttact gattacctta gctaaactaa
     7441 gtgcgcatta cggtttagga ttaaaatctt caatagatat aacaaatgca caattgttta
     7501 gtgagacaca aggccgatat gttgtttctg ttaaatcagg taaaacttta aatattgata
     7561 atgcaataga aattggactt ttaacagata gtgataattt caaagtaaca acaccatata
     7621 cagagattag tgaaaatgtt tcagatatta aacaaatatg ggaaggggca attgctcaat
     7681 gtttaactac tcaggattaa acgaagaatg tggcgtgttt ggtatttgga atcatcctga
     7741 agcagcgcaa ctaacatata tgggacttca tagtttgcaa catcgtggtc aagaaggtgc
     7801 aggtatagtt gtttctgatc aaaatgaatt aaaaggcgag cgaggattag gcttactaac
     7861 tgaagcgatt aaagatgatc aaatggaacg attaaaagga tatcaacatg caattggtca
     7921 cgtccgttat gctacttcag gtaataaagg tattgaaaat attcaaccgt ttctgtatca
     7981 cttttatgat atgagtgtag gtatttgtca taatggtaac ctcattaatg ctaaatcatt
     8041 gcgtcagaat ttagaaaaac aaggtgctat cttccattcg tcttctgata ctgaagtcat
     8101 tatgcatttg attcgacgca gtaaggcacc aacttttgaa gaagcattaa aagaaagtct
     8161 tcgaaaaatt aaaggtggtt ttacatttgc aattttaaca aaggatgcgt tatatggcgc
     8221 agtagatcca aatgctatca gaccacttgt tgtaggtaaa atgaaagatg ggacatacat
     8281 ccttgcaagt gaaacatgtg caatagatgt gttaggtgca gaattcgttc aagatattca
     8341 tgcaggtgaa tatgtcgtga ttaacgataa aggtattaca gttaaatctt atacacatca
     8401 tacgacaact gcaatttctg cgatggaata tatttatttt gctagaccag actcaacaat
     8461 agctggtaaa aatgtccatg cagtacgtaa agcttctggt aaaaaattag cccaagaaag
     8521 ccctgtaaat gctgatatgg tcatcggtgt acccaattca tcgttatcag ctgcgagtgg
     8581 ttatgctgaa gaaataggtt tgccatatga aatgggacta gttaaaaatc aatatgttgc
     8641 aagaacattt attcaaccaa ctcaagaatt acgtgagcaa ggtgtgagag tgaagttatc
     8701 tgcggtaaaa gatatagtag atgggaaaaa tatcattctt gttgatgatt ctattgttcg
     8761 cggtacgaca attcgacgca ttgtgaaaat gttaaaagat tctggtgcaa ataaagtgca
     8821 tgtgcgtata gcatcaccgg aatttatgtt cccaagtttt tatggaatcg atgtttcaac
     8881 tacggcagaa ttaatttctg caagcaaatc acctgaagaa attaaagatt atattggcgc
     8941 tgattcatta gcatatctat ctgtagatgg gttaattgaa tcaattggtt tagattatga
     9001 cgcgccatat agtggcttat gtgtagaaag tttcactgga gattatcctg cagggttata
     9061 cgattatgaa gcaaattata aagcacattt aagtcatcga caaaagcaat atatttctaa
     9121 aaataaacac ttttttgata gtgagggaaa tttaaatgtc taaagcatat gaacaatctg
     9181 gtgtaaatat tcatgctggt tatgaagctg tagaaagaat gtctagtcat gttaaacgta
     9241 cgatgcgtaa agaagttatc ggtggtttag gtggattcgg tgctacattt gatttatcac
     9301 aattaaatat gacagcacca gttttagttt ctggaacaga cggagtagga acgaaattaa
     9361 aactagctat cgactatggt aaacatgatt cgataggtat cgatgcagtc gcaatgtgtg
     9421 ttaatgatat tttaacgaca ggtgcagaac cattatattt tttagattac atcgctacaa
     9481 ataaagttgt tcctgaagtt attgaacaaa ttgttaaagg tattagtgat gcatgtgttg
     9541 aaacgaatac tgcacttatc ggtggagaga ctgctgaaat gggtgaaatg tatcacgaag
     9601 gcgaatatga tgtagccgga tttgctgttg gagcagttga aaaggatgac tatgtagatg
     9661 gttcagaagt gaaagaggga caagttgtta taggacttgc gtcaagtggc attcattcaa
     9721 atggatatag tttagtgcgc aaattaatta atgaatcagg cattgatttg gcatcaaact
     9781 tcgataatcg tccatttata gatgtctttt tagaaccaac taaattatat gtcaaacctg
     9841 tacttgcttt gaaaaaagaa gtttctatta aggcaatgaa tcatattact ggtggaggtt
     9901 tttatgaaaa tattccacgt gcattgccag ccggatatgc tgctagaatt gatactacat
     9961 catttccaac gccaaaaata tttgattggt tacaacaaca aggcaatata gatacaaatg
    10021 aaatgtataa catttttaac atgggtattg gctatacggt tatcgttgat gaaaaagatg
    10081 catcacgcgc tttgaagatt ttagcagaac aaaatgtgga agcctatcaa attggacata
    10141 ttgtgaaaaa tgagtcaact gcaattgaat tgttgggggt ataacaatgg ttaaaattgc
    10201 gatttttgca tcaggttcag gaagtaactt tgaaaatata gttgagcatg ttgaatcagg
    10261 aaaacttgaa aatattgaag ttacggcgct atatacggat catcaaaatg cgttttgtat
    10321 agatagagca aaaaagcacg gtattcctgt ttatattaat gaaccaaaac aatttgattc
    10381 aaaagcagcg tatgaacaac atttagtaac attattaaat aaagataagg tagagtggat
    10441 tattttagct ggttacatgc gtctaattgg tccagattta ttagcttcat ttgcaggtaa
    10501 aatattgaat atacatccat ctctattgcc gaaatataag gggattgacg caataggcca
    10561 agcatatcat agtggcgata ctattactgg ttcgacagta cattatgttg atagtggtat
    10621 ggatacggga gaaattattg aacagagaaa atgtgatatt agaccggacg attcaaaaga
    10681 acaattagaa gagaaagtaa aaaaattgga atatgagtta tatccaagtg ttattgctaa
    10741 aattgtaaaa taaggagtat gtatgtgatg aagaaagcta ttttgagcgt atcaaataaa
    10801 acaggtattg tagagtttgc taaagcgtta acgcaattaa attatgaatt atattcaaca
    10861 ggtggtacta aacgtatatt agatgaagca aatgtaccag ttcgttctgt ttcagactta
    10921 acacattttc cagaaataat ggatggccgt gttaaaacat tacatccggc tgttcatggt
    10981 ggcattttag ctgatcgaaa taaaccgcag catttaaatg aattatcaga acaacatata
    11041 gatttaattg atatggtagt agttaattta tatccattcc aacaaactgt tgcaaaccct
    11101 gatgtgacga tggacgaagc aattgaaaat attgatattg gtggtccaac aatgttacgt
    11161 gctgcagcta aaaactataa acatgtaaca acaattgtac atccggcaga ttatcatgaa
    11221 gtattgacgc gattaagaaa cgattcgtta gatgagtcat atagacaatc attaatgatt
    11281 aaagtttttg agcatactgc agaatatgat gaagcgattg tacgtttctt taaaggggat
    11341 aaagaaactt taagatatgg agaaaatcca caacaatcag cgtattttgt gagaacttcg
    11401 aatgctaagc acacgattgc aggcgctaaa caattacatg ggaaacaatt aagctataac
    11461 aatattaaag atgcagatgc tacactagct ttagttaaaa agtttgatac acctgctgca
    11521 gttgcggtta aacacatgaa tccatgtggt gttggtatcg gtgacacgat agagcaagca
    11581 tttcaacatg cgtatgaagc ggatagtcaa tcaatatttg gtggaattgt tgcattaaac
    11641 cgagctgtaa cacctgagtt agcagagcaa ttgcatagta tctttttgga agtcattatt
    11701 gcaccaaaat ttacagatga agcattagat attttaaaac aaaagaagaa tgtaagatta
    11761 ttagaaattg atatgactat agacagtaac gaagaagagt ttgtttcagt atctggcgga
    11821 tatttagttc aagataaaga taattatgtc gtaccaaaag atgaaatgaa agttgttaca
    11881 gaagtagtac ctacagatga acaatgggaa gcaatgttat taggatggaa agttgtacca
    11941 tcagtaaaaa gtaatgcaat tattttaagt aataataaac aaactgtagg tataggtgct
    12001 ggacaaatga atcgtgtcgg tgctgctaaa attgcgttag agagagctat tgaaatcaat
    12061 gatcatgtag cgttagtatc tgatggattt ttccctatgg gagatacagt tgaacttgca
    12121 gcacaacatg gtataaaggc aattatccaa ccgggtggtt cgattaaaga tcaagattca
    12181 attgatatgg ctaataaaca tggtattgca atggtagtca caggcactcg acattttaaa
    12241 cactaatttt aaaggggcgt aacataaatg aatatattag taattggtgc tggtggacga
    12301 gaacatgcac ttgcatataa acttaatcaa tcgaatctag ttaaacaagt gtttgtcatt
    12361 ccaggtaatg aggcaatgac acctatagct gaagtacaca ctgaaatttc agaatctaat
    12421 catcaaggga tactagattt tgctaaacag caaaatgttg attgggtagt tataggtcca
    12481 gaacagccgc taattgatgg attagcagac attttacgag cgaatggttt caaagtgttt
    12541 ggtccaaata agcaagcagc tcaaatcgaa ggctcaaaat tatttgctaa aaagataatg
    12601 gaaaaatata atattccaac tgctgattat aaagaagttg agcgaaaaaa ggatgcttta
    12661 acatatattg aaaactgtga attgcccgtt gttgtcaaga aagatgggtt agctgctggg
    12721 aaaggcgtta ttattgcaga tactattgaa gcagccagaa gtgctattga gattatgtat
    12781 ggtgatgaag aagaaggtac tgttgtattt gaaacgtttt tagaaggtga agagttctcg
    12841 ctaatgacat ttgttaatgg tgatttagca gttcctttcg actgtattgc acaagatcat
    12901 aaacgcgcat ttgatcatga tgaaggacca aatactggtg gtatgggggc ttattgtcca
    12961 gtaccacata ttagtgacga tgttttaaaa cttacaaatg aaacaattgc acaacccatt
    13021 gcaaaggcaa tgcttaatga aggttatcaa ttcttcggtg tattatacat tggtgctatt
    13081 ttaactaaaa atggtccaaa agtaatagaa tttaatgccc gttttggtga tcctgaagct
    13141 caagtattat taagtcgcat ggaaagtgat ttaatgcagc atattattga tttagatgaa
    13201 ggaaaacgta ctgaattcaa atggaaaaat gaatctattg taggggtcat gttggcatca
    13261 aaaggatatc ctgatgcata tgaaaaaggg cataaagtaa gtggctttga tttaaatgaa
    13321 aactattttg ttagtggatt aaagaagcaa ggtaatacct ttgttacttc aggtggtaga
    13381 gttatacttg ccatcggaaa aggtgacaat gtacaagatg cacagcgaga cgcatacgaa
    13441 aaagtatcac aaatacaaag tgaccattta ttctatcgtc atgacattgc gaataaagca
    13501 ctacaactta aataagtata tttaaaaaat actaagatta gctatgaaga aatctataac
    13561 gatagttttt tcatagcttt tttagttgta gagtctagga cattgatttc tgtaccaaat
    13621 ttgtgattat tcatatgtaa tacaaaagag gcgccacaac atgtttggat gaacaaaata
    13681 acatgtttgt ggcacctctt ttgtttagta tggaataaat ggttttcttt ttctatacaa
    13741 tgaatttcta atttagtatc tatacaatta tggataaaat ttaacctaca cgaccaagac
    13801 gaacatcatc tatgcccgtg atgggtaagg tgattgaaca ataatatgcc aaagtaataa
    13861 tggcaattaa aactataata aagattatat ctttatatga gaaaggtacg ttgtaatagt
    13921 aagtacgagg accatctcta aatcctttcg actccatcgc aactgataat tgatgtgcct
    13981 ttctaatatt ttggcttaat agaggtataa ttaaatgctt aaatcgcttt aaccctctat
    14041 aatttgccgc gtctatcatc tgatagcgca tttttaaaga tctgcgaagc tgtattaaag
    14101 aactaatcat taaaggtatc atacgaatgg cagccatgaa tgcataagca acttttgatt
    14161 taacctttaa atgctgcatt aaactataaa atatcatgac aacctgagat gtaagtgcga
    14221 ttaagatacc gaaaaatgat atagcaatgg ttcttaatga tacatgtaaa ccacgaacta
    14281 aactttctgt tgtaatatgg ataaatccga atttcaaaat tgtatggcta ccattcccgt
    14341 ataaaatcat gaatagggaa gagagtaatg caaagccaat acttatagtt ataaaaattg
    14401 atgtaatttt aaactgagta ccattaaaca tcaataagaa aactaacatt aagatagtga
    14461 tataaagcat aaaatcgaaa ttatgcacaa atataataaa gaaaaatagt ataattccaa
    14521 gaaatagttt cgttataatg ttgacatcat caacaaatga ttgccgaact ttccattgct
    14581 catacattcg tatcaccatc acaatctagt aacgtaccat ctgaaatttt aagtcttctt
    14641 gatggataac gttcaattat ttcatcgtca tgtgtaacca tgacaatact ttgtcccaaa
    14701 ttaattcgtt tttggaaaag tttgatcaac tggaatgtat tatggctatc aagtccaaat
    14761 gtcggttcat ctaaaaagat gatatcagct ttagaactta gtgcggtagc tacgctaagg
    14821 cgtcgttttt gaccaataga caactcataa ggatgttgat ctttaacatt ttctaaatct
    14881 aaaagtttta aaagttgtat cgtttcatca tcactttgat ctttagaaag gtgattaaaa
    14941 tgaatgttaa tttcatcata aaccgaattt gttataaatt gtaattctgg gttttgataa
    15001 actagataca tgtgttttgc tgcatgttta atttttgtta aacgttgatt ttcaaaataa
    15061 acatcacctt gatatttaat caattgcata attgattcaa gcaaggtagt tttaccactg
    15121 ccatttgccc ctgtaattgt aatccactca cctaaaccaa tttctaaatc tgagaatgaa
    15181 agcaacgttg atttaccgcg aataatacgt ccatttttaa attgtaataa gtgtgagttt
    15241 gttgttggaa agtcaacacg acttggtgcg aattcccatg cacgtggatg ccacacaccg
    15301 tattcactga gtaaatgaac atacttctgt aatatgattt caggacattc atcggcaatg
    15361 atatttccgt tataatccat caaaatgacg cggtcgacat gattccagat gtgtttaact
    15421 ttatgttcaa cgattacaac agtttgatct tcccaaagtt caattagttt agtccataaa
    15481 tcttccgttg cttgaacatc taacattgct gtcggttcat ctaaaaacaa tgtttttgat
    15541 tgttgaagaa tggtttcaac aattgccaat ttctgtttca tcccgccact taaatctttg
    15601 atatacgttt caggggtaac atttaaattg accatattta aagcattgat aattaacgca
    15661 tccatgtctt cacgtggtac ttgtctattt tctaaaacga atgcaagttc ttcgtatact
    15721 tttggcatac aaaactggct atcagggtct tggaaaataa cgccacttaa tgggtcaacg
    15781 attagttcat catatttcat aggtaattca attaaattag gaacaatacc acttaataca
    15841 ttcagaagtg tacttttacc gcaaccagaa ggaccgagta aaagtacttt ttctttgtct
    15901 tgaatagtga tatttaaatg atcgaaaatt ttacgctgac cacttggata ttttaatcgt
    15961 aaatcactta cttttaacac tctgaatgct cctcttataa gttgtcgtaa tcttctttag
    16021 cagctggtct aaataatttt gttacgcctg tcttatctaa agcttttact aaaaggtaag
    16081 ataggacgcc ggcgactact gcaccactaa ttaatctaaa tacgatgaat aatgttaagt
    16141 tccaacctgc aacttcattt aaatacccat agaaataatc tatcgggaaa gctgcgattg
    16201 ctgtacaaaa acctgctaac atagctacca taactgaacg tgattgatat ttaaaaattg
    16261 caaagacaag ttcacacgct aaaccttgta taaaagcgta aacgattgtc ggaatatcga
    16321 aacgacccat aatgatagtt tcgcctgcac ctgcagcaaa ttcagccagt aaagcaatac
    16381 ctggctttgg gataattaga tagcagacaa tagctgccat gaaccaaacc ccgtttgtta
    16441 attgttcgag gtgaaggcct gtagcttgca caccattgta aacaaaccac cataaattgt
    16501 aaataactgc gaatactact gaaataagaa cggttactag tatttcagat agctttaaac
    16561 cttttgacat ttttacatcc tcctaataaa aaaacgcaca accatccata ggaaagttat
    16621 gcgttcacaa tatatattag taaaacatat gtatagtaac actttcctac gctagttcaa
    16681 gctagatcag gttcaaaggg tttgagggca agcctcatct cagtataaaa cacccctagt
    16741 gtgtgcgatt tatttaatta attatactgt aagacgtttg taaacttatg tcaataggtt
    16801 gtcttcatga aatttcgttt aattcgattt agaatttata atattggcat tggatttaaa
    16861 ttgaagatgt agtaggaatg ttagtaatta aagatataaa aatatgtgac atgtaataat
    16921 attgagctga taaatgaaga gggatacatg tgccgagcgc aagcaaaaaa gcccctaaca
    16981 actaaagctt gtaaggtagg agagggatac atgcgccgag cacatgcaaa aaagccccta
    17041 acaactaaaa gttgtaaggg gctctaaatt aatttagtgt atcttggata tcttgttttg
    17101 tttgattaat atcttctgtt tttacttctt ttttatcttt taatttttct tcaacttctt
    17161 tagctttttc tgctgctttt ttattttgat tttcattaga catgattaat tcctcccaaa
    17221 ttggataatt atttatatat aaatcttacc cggttgtact ttcgttaaac ttttctaagt
    17281 ctatagcact atttattcat ttatctaaag acaacaacat tagattaata tataatgatt
    17341 ttgaggtgaa cataatgtct tttcttagga aacacgccga aattattttt agctatttaa
    17401 tcggtatcgt ttcactcttc actggtctca ttattttaat taacttgcca ttaattaaac
    17461 aattaaatgg tggtaaaaaa gttgatacac atgttcataa tgtgtgggaa tttctgaatg
    17521 catttttcag tgaaattatt aaagtaatga gtcgatttat aggtaatttc cctatagtta
    17581 gtgcaattgt gataattata ttcggtattt tagttatgtt gattggtcat acattactta
    17641 gaactattaa gtatgactat gatatttcta tctttttctt agttatcggt atcatgtatt
    17701 ttattattac tcttatatta atgactcaag tttatggatt ctttgcagtg attttcatta
    17761 ttccatttac aattcatata ggatatatcg tctataaaga tgaattgaat caggaaaatg
    17821 taaaaaatca tttcatgtgg ataattgtga gttatggtat aagttactta attacacaaa
    17881 ttgcattgta tggcagaatt gatgctaatg aaatagagtc aattgatatc ttaagtgtca
    17941 atgctttctt tataattatg tggttacttg gtcaaatggc tatttggaat ttcttgttct
    18001 tgcgccgagc tttaccttta acaaagcaag aattaggtga agaggagcca gaattatcaa
    18061 gaacaagtaa agggaatgtc acgaatcaaa ctaaaattca cttgaaacaa ctccaagata
    18121 agactacaga atatgcacgt aagacaagaa gaagtgtcga tttagataaa attagagcta
    18181 aaagagataa attcaaaaag aaagttaatg atattatcga tattcaagaa gacgatattc
    18241 ctgattggat gagaaaaccg aaatgggtta aaccaatgta tgtcgaacta ttttgtggtg
    18301 tcgtcatctt tttattcaca tttttagaat ttaataatcg taatgcatta tttgtatatg
    18361 gtgattggaa attatcacag acacaatatg ttattgaatg ggttacatta ctaattctgt
    18421 tattcattat tatcgcatat atcgctacaa cgttaacttt ccacttgaaa ggtaagtttt
    18481 attatttaca actatttatg gggagcattt tattctttaa attgttaacg gaatttataa
    18541 atataatgat tcacggacta ctactttcag tgttcattac gccaacatta ctattaatgt
    18601 tattggcaat catcatttct tattcgttac aattacgaga gcgaccataa ttaaaagcat
    18661 tataaaagta ctatctatta aacattttga tgtgtacgct ataagttaga tatatctcta
    18721 acttacttag atacatgtca atgaagttta tggatagtac tttttttgta actagatttg
    18781 attgattcag gtgatgtgaa ttaagtattg aaaattgtat acaaagtttt agtgcaaata
    18841 aaatagttga aaagttatcc atttgtaaaa tcaagaaaac tagtaaatag ttgaagcgac
    18901 ttatggaatt tgcgaaacga tatatagtat ttcctttgta gaaatttaac atatatcatt
    18961 cgaattacta atttgttaaa atcaacagta agattagaag tagatgatat tgaaatttgg
    19021 caaacaattt aatctatata aaactacaac gaaacacaga aaggaagttg tcagatgaaa
    19081 atagcaactc tgaacaaagg caaagaaaca aaatatttta atggatatcc tttaattgaa
    19141 gaagaggata tctattcaca agatcattta aaagaaggag atatttttca aattgtgact
    19201 gataaatcac aatatgttgc aacggcttat gttggtcgcc aacataaagg attaggttgg
    19261 gttctaacat acgataaagc tcaagaaatc aacacagctt tctttgtgaa attgtttaat
    19321 actgcattag cagaacgtga ttattatttt aatatagatg gaacaaatgc ttttagatta
    19381 tttaatgctg aaggtgatgg tgttggggga ttaacaatcg acaattacga tggtcatttg
    19441 ttgattcaat ggtactcaaa aggtatttat aaatttaaat atgccattct tgaagcggtt
    19501 agaaaagtat ttgattataa atctatttac gaaaaagtaa gatttaaaga cagcgaatat
    19561 agtggtggtt ttgttgaagg cgatgcacct gagtttccaa ttgttatcga agaaaacttc
    19621 acattttata atgtagacct tgaagatggt ttgatgacag gtatcttttt agatcaaaaa
    19681 gaagtgcgca agaaattaag ggatcaatat gccaaagaac gccatgtttt aaacttattt
    19741 agttatacag gtgctttttc tgtaatagca gcaagtgagg catcttcaac aacaagtgta
    19801 gatttggcta atcgttctcg tagtttaact gaagaaaatt ttggattaaa tgctattgat
    19861 cctaaatctc aatatattta tgtcatggac acttttgatt tctataaata tgctgcacga
    19921 catggacata gttatgacac aatcgtgatc gatccaccta gctttgcacg caacaaaaaa
    19981 cgtacatttt cagtgcaaaa agattatgac aaattaatta ctggcgcctt aaatatctta
    20041 tcatctgaag gaacattatt attatgtaca aacgcaagtg tatatccatt aaagcaattt
    20101 aaaaatacta ttaaaaagac gcttgaagag agtggcgttg attatgaatt aactgaagtt
    20161 atgggattac caaaagattt taaaacacat ccacattata agccatctaa atatttaaaa
    20221 gctgtttttg taaatattag acattaattt gataaaaagg ggtatagtat cttattgaga
    20281 aaaagaaggg tgatgataca atgggattca aaaacaattt aacatcaaat ttaacaaata
    20341 aaattggtaa ttcagttttt aaaatagaaa atgttgacgg aaaaggtgca atgccaacga
    20401 cgattcaaga attgagagaa agacgcgaac gtgctgaagc aattgtaaag agaaagtctt
    20461 taatgtcatc aacaatgagc gttgttccaa ttccgggttt agattttggt gttgatttaa
    20521 aattaatgaa agatattatc gaagatgtaa ataaaatata cggattagat cataagcaag
    20581 ttaatagcct tggggatgat gtaaaagaaa gaattatgtc tgcagcagca attcaaggta
    20641 gtcaatttat tggtaaaaga atttcaagtg catttttaaa aattgtaatt agagatgtag
    20701 caaaacgaac tgcagctaaa caaacaaaat ggtttcctgt tgtaggacaa gctgtgtctg
    20761 catctattag ttactatttt atgaataaaa ttggaaaaga tcacattcaa aaatgcgaaa
    20821 atgttattaa aaatgtcatg taggtgctat aatagttttg caatttgcaa attttactga
    20881 aaccggtttt aaacgaattg aatttaaagc atggttttgg taaagttaag gtataaaact
    20941 aagttagtat tgtaataata ttgaagattc taactatacg aaggagaaat gtaattatgg
    21001 aacaaaattc atatgtaatc atcgacgaga ctggtattca cgctagacca gcaacaatgt
    21061 tagtacaaac agcttcaaaa ttcgattctg atattcaatt agaatataac ggtaaaaagg
    21121 ttaacttaaa atcaatcatg ggtgttatga gccttggtgt tggtaaagat gctgaaatta
    21181 caatttatgc tgacggtagt gatgaatctg acgccattca agcaatcagt gacgtcttat
    21241 caaaagaagg attgactaaa taattatgtc taaattaatt aaaggaattg ctgcatctga
    21301 tggtgttgca attgctaaag cttatttgtt agttgagcca gacttaacat tcgacaaaaa
    21361 tgaaaaagtc actgatgttg aaggagaagt tgctaagttc aataacgcta tcgaagcttc
    21421 taaagttgag ttaactaaaa ttagaaataa tgcagaggtt caattgggtg ctgataaagc
    21481 tgctatcttt gatgcacatt tattagtttt agatgaccct gaattaattc aaccaattca
    21541 agataagatt aaaaatgaaa acgctaatgc tgcttcagca ttaacggatg taacaacaca
    21601 atttgttaca atttttgaat ctatggataa cgaatacatg aaagaacgtg cggctgatat
    21661 tcgcgacgtt tctaaacgtg tgttatcaca tattttaggt gtagaattac cgaatccgag
    21721 tatgattgat gaaagcgttg ttattgtagg gaatgactta acgccatctg atactgctca
    21781 attaaataaa gaattcgtac aaggttttgc tacaaacatt ggcggaagaa caagtcactc
    21841 tgcaattatg agtcgttctt tagaaattcc agcaattgtt ggtacaaaat caattactca
    21901 agaagttaaa caaggcgaca tgattatcgt agatggatta aatggtgatg taatcgttaa
    21961 tccaactgaa gatgagttaa tcgcttatca agataaacgt gagcgttatt ttgctgacaa
    22021 gaaagaatta caaaaactac gtgatgctga tactgttaca gttgatggtg ttcacgcaga
    22081 gcttgctgca aatattggta cacctaatga tttgccaggt gttattgaaa atggtgcaca
    22141 aggtatcggc ttatatagaa ctgagttttt atatatgggt cgtgaccaaa tgcctacaga
    22201 agaagaacaa tttgaagctt ataaagaagt attagaagca atggacggta aacgtgttgt
    22261 tgtacgtact ttagatatag gtggagataa agaattatca tacttaaact tgcctgaaga
    22321 aatgaatcca ttcttaggtt accgtgcgat tcgtttatgc cttgcgcaac aagatatttt
    22381 cagaccacag ctacgtgcat tattacgtgc atcagtttat ggtaagttaa atatcatgtt
    22441 cccaatggtt gcaacaatta acgaatttag agaagctaaa gctatattat tagaagaaaa
    22501 agaaaacctt aaaaatgaag gtcatgacat ttcggatgat atagaattag gaatcatggt
    22561 agagatacct gcaacagcag cattagctga tgtttttgct aaagaagtag atttcttcag
    22621 tatcggtaca aatgatttaa ttcaatacac attagctgct gaccgtatgt cagagcgtgt
    22681 atcatatctg taccaaccat ataacccttc aatcttacgt ttagttaaac aagttattga
    22741 agcgtcacat aaagaaggta aatggacagg tatgtgtggt gaaatggctg gagatgaaac
    22801 agctattcca ttattgcttg gtttaggttt agatgagttc tctatgagtg caacgtctat
    22861 tctgaaagca agaagacaaa ttaatggttt aagtaaaaat gaaatgactg aacttgctaa
    22921 ccgtgcagtc gactgtgcaa cgcaagaaga agttattgaa ttagttaaca actacgtaaa
    22981 ataattaatt ctttcgaaag acagaattat aaataaaccc gaagtattca ttgtgagtaa
    23041 ttatgaatac ttcgggttct tttttatgat ttat
//
LOCUS       DAHHOJ010000035        22942 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.035, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000035 DAHHOJ010000000
VERSION     DAHHOJ010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22942
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.035"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..734)
                     /gene="ccsA"
                     /locus_tag="NBH32_001162"
     CDS             complement(<1..734)
                     /gene="ccsA"
                     /locus_tag="NBH32_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HCD1514779.1"
                     /translation="MQENLFIRFNEIILLIYLISIICYFYDFVQKSHKIRSLGIYLLG
                     IVWVLQTISLSIFIIQTRHIPLGSISDVFYTLSWLIISISLILNLIKVLNFSVFFLNL
                     IGLTLLGMNTFQPTHYTNKVQKIAVVDELLLVHIGLAVLSYVFFALAFVNALLYIMQY
                     RNLKEKRFDQKYFRIGSVATLESIVFYSTLSGWIILIFSIILGTQWGVISVGERIFID
                     PKVILSSIITVLYGSYILLRINKWLNS"
     gene            complement(756..2102)
                     /locus_tag="NBH32_001163"
     CDS             complement(756..2102)
                     /locus_tag="NBH32_001163"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCD1514780.1"
                     /translation="MHFIAISINHRTADVALREQVAFRDDALRIAHEDLYETKSILEN
                     VILSTCNRTEVYAVVDQIHTGRYYIQRFLARAFGFEVDDIKAMSEVKVGDEAVEHLLR
                     VTSGLDSIVLGETQILGQIRDAFFLAQSTGTTGTIFNHLFKQAITFAKRAHNETDIAD
                     NAVSVSYAAVELAKKVFGKLKSKQAIIIGAGEMSELSLLNLLGSGITDITVVNRTIEN
                     AMKLAAKHQVKYDELSSLPNLLESADIVISSTSAQSYIITNEMIERIAENRKQDSLVL
                     IDIAVPRDIEPGISAITNIFNYDVDDLKGLVDANLRERQLAAATISEQIPAEIHAHNE
                     WISMLGVVPVIRALREKAMTIQAETMDSIDRKLPGLSERERKIISKHTKSIINQMLKD
                     PIKQAKELSSDKKSNEKLELFQNIFDIEAECPHEQAKQQKESKVKEISARRIFSFE"
     gene            complement(2319..2909)
                     /locus_tag="NBH32_001164"
     CDS             complement(2319..2909)
                     /locus_tag="NBH32_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCD1514781.1"
                     /translation="MKVNPNNIELIISAVKEEQYPETELSEVALSGRSNVGKSTFINS
                     MIGRKNMARTSQQPGKTQTLNFYNIDEQLIFVDVPGYGYAKVSKTQREKFGKMIEEYI
                     TKRENLQLVIQLVDLRHDPTQDDILMYNYLKHFDIPTLVICTKEDKIPKGKVQKHIKN
                     IKTQLDMDPDDTIVSYSSIKNNKQQQIWNLIEPYIS"
     gene            complement(3063..4325)
                     /gene="clpX"
                     /locus_tag="NBH32_001165"
     CDS             complement(3063..4325)
                     /gene="clpX"
                     /locus_tag="NBH32_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HCD1514782.1"
                     /translation="MFKFNEDEENLKCSFCGKDQDQVKKLVAGSGVYICNECIELCSE
                     IVEEELAQNTSEAITELPTPKEIMDHLNEYVIGQEKAKKSLAVAVYNHYKRIQQLGPK
                     EDDVELQKSNIALIGPTGSGKTLLAQTLAKTLNVPFAIADATSLTEAGYVGDDVENIL
                     LRLIQAADFDIDKAEKGIIYVDEIDKIARKSENTSITRDVSGEGVQQALLKILEGTTA
                     SVPPQGGRKHPNQEMIQIDTTNILFILGGAFDGIEEVIKRRLGEKVIGFSSNEADKYD
                     EQALLAQIRPEDLQAYGLIPEFIGRVPIVANLETLDVTALKNILTQPKNALVKQYTKM
                     LELDDVDLEFTEEALSAISEKAIERKTGARGLRSIIEESLIDIMFDVPSNENVTKVVI
                     TAQTINEETEPELYDAEGNLINNSKTSA"
     gene            complement(4476..5777)
                     /locus_tag="NBH32_001166"
     CDS             complement(4476..5777)
                     /locus_tag="NBH32_001166"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCD1514783.1"
                     /translation="MTATWEKKEGNEGLLTVTVPAEKVNKALDQAFKKVVKQINVPGF
                     RKGKVPRPIFEQRFGVEALYQDAIDILLPDAYGEAIDETDIKPVAQPEVSVTQIEKGK
                     DFIFEATVTVEPEVKLGDYKGLEIEKQETELSDDELQEAIDHSLGHLAEMVVKEDGVV
                     ENGDTVNIDFSGSVDGEEFEGGQAEGYDLEIGSGSFIPGFEEQLEGMKVDEEKDVVVT
                     FPEEYHAEELAGKEATFKTKVNEIKFKEVPELTDEIANELDAEANTVDEYKENLRKRL
                     AEQKATDAENVEKEEAITKATDNTTIDIPEAMVNTELDRMVSEFAQRIQQQGLDLQTY
                     FQISGQDESQLREQMKDDAEQRVKTNLTLTAIAEAEKIEATDEDIDKELEKMSKQFNI
                     SVEDIKNTLGNTDIIKNDVRIQKVIDLLRDNAKFVEGTKED"
     gene            complement(5825..5935)
                     /locus_tag="NBH32_001167"
     CDS             complement(5825..5935)
                     /locus_tag="NBH32_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514784.1"
                     /translation="MQNNTQHFNIVLTKKVDLQIYKRCDFNVCIGNRPMI"
     gene            complement(5940..6869)
                     /locus_tag="NBH32_001168"
     CDS             complement(5940..6869)
                     /locus_tag="NBH32_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514785.1"
                     /translation="MSDIIQFPNSSIKLYKDIKRAEQDQNYDLMYEYIVQYERQFELT
                     EEIAMMKCRMLYETGSFLELREETIVLLKTGIQQYDALMIYYVKSLIGLGQYFEAVEV
                     IHQIIDEVKDHKTRMALHPLKEFAKSKLIEDEKRLTQSLTDFDTLSMREQTHLILKLI
                     DIGHFQFQETVLYILKSNTYSYNLISLMIEYLRFANCTQELTIEKYGMDVTFVPANLN
                     GLEHTTLKEKVIPNVMETLNDGALHIAEEAHHIMNNHSIMIYPIDIETLFETNKWINA
                     YECYFKNMLGLKCELQSIDAFNFIQQLDLNNNS"
     gene            complement(6888..7496)
                     /locus_tag="NBH32_001169"
     CDS             complement(6888..7496)
                     /locus_tag="NBH32_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HCD1514786.1"
                     /translation="MSKFDEQIIVVPREIIFNNEKNTFNGFLNKNKPEGQNIFDALSQ
                     YEVKRRGDMEEDPSYKQLISYCLLENEHGEILVYERLSGGGEARLHGQSSIGVGGHMN
                     DVPGAESINEVLRVNAQRELEEEVGLSEQDSQNMEYIGFINDDNNEVGKVHIGVVFKI
                     TVSTNDVEAKETDTLRIKWVEKGNIESYDDFETWSALILQDL"
     gene            complement(7638..7994)
                     /gene="rplT"
                     /locus_tag="NBH32_001170"
     CDS             complement(7638..7994)
                     /gene="rplT"
                     /locus_tag="NBH32_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCD1514787.1"
                     /translation="MPRVKGGTVTRARRKKTIKLAKGYFGSKHTLYKVAKQQVMKSGQ
                     YAFRDRRQRKRDFRKLWITRINAAARQHEMSYSRLMNGLKKAGIDINRKMLSEIAISD
                     EKAFAQLVTKAKDALK"
     gene            complement(8041..8241)
                     /gene="rpmI"
                     /locus_tag="NBH32_001171"
     CDS             complement(8041..8241)
                     /gene="rpmI"
                     /locus_tag="NBH32_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCD1514788.1"
                     /translation="MPKMKTHRGAAKRVKRTASGQLKRSRAFTSHLFANKSTKQKRQL
                     RKARLVSKSDMKRVKQLLAYKK"
     gene            complement(8270..8797)
                     /gene="infC"
                     /locus_tag="NBH32_001172"
     CDS             complement(8270..8797)
                     /gene="infC"
                     /locus_tag="NBH32_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCD1514789.1"
                     /translation="MSTIAKDQTQINDKIRAKELRLIGQDGEQIGVKSKREALEMAER
                     VDLDLVVVAPNAKPPVARIMDYGKFKFEQQKKEKEMKKKQKIINVKEIRLSPTIEEHD
                     FQTKLKNGRKFLTKGDKCKVSIRFRGRAITHKEIGQRVLEKYADECKDIATVEQKPKM
                     DGRQMFIMLAPTAEK"
     misc_feature    complement(8826..8951)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            complement(9026..10519)
                     /locus_tag="NBH32_001173"
     CDS             complement(9026..10519)
                     /locus_tag="NBH32_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1514790.1"
                     /translation="MSKVQNESNNVVKRGLKDRHISMIAIGGCIGTGLFVTSGGAIHD
                     AGALGALIGYAIIGIMVFFLMTSLGEMATYLPVSGSFSTYATRFVDPSLGFALGWNYW
                     FNWVVTVAADITIAAQVIQYWTPLQGIPAWAWSALFLVIIFSLNSLSVRVYGESEYWL
                     ALIKVVTVIVFIAIGLLTIVGIMGGHVVGFEIFNKGEGPILGGNLGGSLLSILGVFLI
                     AGFSFQGTELIGITAGESENPERAVPKAIKQVFWRILLFYILAIFVIGMLIPYDSSAL
                     MGGSDNVATSPFTLVFKNAGFAFAASFMNAVILTSVLSAGNSGMYASTRMLYSMSKDK
                     LAFEAFGKTNKNGVPYMSLLVTAIIVVIIFVLQSLTKGAYEYIVAASGLTGFIAWVGI
                     AVSHYRFRRAFDKQNYDKSKLKYTAKLFPFGPLFAGFLCIVVIIGQDVDFIKTGDFNF
                     SRFLITYMGIPVFLAFFIYHKLRYKTKKIPLEQVDLRQDVSMDEIKG"
     gene            complement(10945..12882)
                     /gene="thrS"
                     /locus_tag="NBH32_001174"
     CDS             complement(10945..12882)
                     /gene="thrS"
                     /locus_tag="NBH32_001174"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCD1514791.1"
                     /translation="MEQINIQFPDGNKKAFDKGTTTEDIAQSISPGLRKKAVAGKFNG
                     QLVDLTKPLETDGSIEIVTPGSEEALEVLRHSTAHLMAHAIKRLYGNVKFGVGPVIEG
                     GFYYDFDIDQNISSDDFEQIEKTMKQIVNENMKIERKVVSRDEAKELFSNDEYKLELI
                     DAIPEDENVTLYSQGDFTDLCRGVHVPSTAKIKEFKLLSTAGAYWRGDSNNKMLQRIY
                     GTAFFDKKELKAHLQMLEERKERDHRKIGKELELFTNSQLVGAGLPLWLPNGATIRRE
                     IERYIVDKEVSMGYDHVYTPVLANVDLYKTSGHWDHYQEDMFPPMQLDETESMVLRPM
                     NCPHHMMIYANKPHSYRELPIRIAELGTMHRYEASGAVSGLQRVRGMTLNDSHIFVRP
                     DQIKEEFKRVVNMIIDVYKDFGFEDYSFRLSYRDPEDKEKYFDDDDMWNKAENMLKEA
                     ADELGLSYEEAIGEAAFYGPKLDVQVKTAMGKEETLSTAQLDFLLPERFDLTYIGQDG
                     EHHRPVVIHRGVVSTMERFVAFLTEETKGAFPTWLAPKQVQIIPVNVDLHYDYARQLQ
                     DELKSQGVRVSIDDRNEKMGYKIREAQMQKIPYQIVVGDKEVENNQVNVRQYGSQDQE
                     TVEKDEFIWNLVDEIRLKKHR"
     gene            complement(13080..13202)
                     /locus_tag="NBH32_001175"
     CDS             complement(13080..13202)
                     /locus_tag="NBH32_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514792.1"
                     /translation="MFDENMVNKLGDYYFIYGICNEYPDQDRYLKQRYLIHKLY"
     gene            complement(13295..14215)
                     /gene="dnaI"
                     /locus_tag="NBH32_001176"
     CDS             complement(13295..14215)
                     /gene="dnaI"
                     /locus_tag="NBH32_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HCD1514793.1"
                     /translation="MKQFKSIINTSQDFEKRIEKIKKEVINDPDVKQFLEAHRAELTN
                     AMIDEDLNVLQEYKDQQKHYDGHKFADCPNFVKGHVPELYVDNNRIKIRYLQCPCKIK
                     YDEERFEAELITSHHMQRDTLNAKLKDIYMNHRDRLDVAMAADDICTAITNGEQVKGL
                     YLYGPFGTGKSFILGAIANQLKSKKVRSTIIYLPEFIRTLKGGFKDGSFEKKLHRVRE
                     ANILMLDDIGAEEVTPWVRDEVIGPLLHYRMVHELPTFFSSNFDYSELEHHLAMTRDG
                     EEKTKAARIIERVKSLSTPYFLSGENFRNN"
     gene            complement(14215..15615)
                     /locus_tag="NBH32_001177"
     CDS             complement(14215..15615)
                     /locus_tag="NBH32_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation and membrane attachment
                     family protein"
                     /protein_id="HCD1514794.1"
                     /translation="MGRQAFEFGLRPKDQFKVMQHFDLNTNHLEVLNRLYTPLIGTQA
                     VGLYHFMTQFVKESHNETLILSHYIFMNELKINLLEFRQQMDLLEAIGLLKAFVKHDE
                     QETQFVYQLIQPPSAHLFFNDPMLSIFLYSEVEHRRFHELKKYFEYQQIDLSEFKQVT
                     RQFTDVFKVPSTKIDIDTSDIPINEPYQGIDLSNESFDFEMLRQMLGKHFISQDIVTK
                     DAKRLITQLATLYGLTADGMKHVILNSITSGQQLSFEEMRKQARSYYLMEHENQMPKL
                     QVKSPVTSSSTGKSSEVNPKPQSDEWFELLEQTSPIDMLASWSESEPTISQKTMVEEL
                     IEREKMSFGVINILLQFVMLKEDMKLPKAYILEIASNWKKKGIKTAKEAYNYAKKVNQ
                     PKNEGSSGNYQKRGSYYGQRNRISKEKTPKWLENRDKPSEEDSAKDNSVDDQQLEQDR
                     QAFLDKLSKKWEEDSQ"
     gene            complement(15616..16086)
                     /gene="nrdR"
                     /locus_tag="NBH32_001178"
     CDS             complement(15616..16086)
                     /gene="nrdR"
                     /locus_tag="NBH32_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HCD1514795.1"
                     /translation="MKCPKCNSTQSKVVDSRHADELNAIRRRRECENCGTRFTTFEHI
                     EVSQLIVVKKDGTREQFSREKILNGLVRSCEKRPVRYQQLEDITNKVEWQLRDEGHTE
                     VSSRDIGEHVMNLLMHVDQVSYVRFASVYKEFKDVDQLLASMQGILSENKRSDA"
     gene            complement(16296..17321)
                     /gene="gap"
                     /locus_tag="NBH32_001179"
     CDS             complement(16296..17321)
                     /gene="gap"
                     /locus_tag="NBH32_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1514796.1"
                     /translation="MSTNIAINGMGRIGRMVLRIALQNKNLNVVAINASYPPETIAHL
                     INYDTTHGKYNLKVEPIENGLQVGDHKIKLVADRNPENLPWKELDIDIAIDATGKFNH
                     GDKAIAHIKAGAKKVLLTGPSKGGHVQMVVKGVNDDQLDIEAFDIFSNASCTTNCIGP
                     VAKVLNNQFGIVNGLMTTVHAITNDQKNIDNPHKDLRRARSCNESIIPTSTGAAKALK
                     EVLPELEGKLHGMALRVPTKNVSLVDLVVDLEKEVTAEEVNQAFENAGLEGIIEVEHQ
                     PLVSVDFNTNPHSAIIDAKSTMVMSGNKVKVIAWYDNEWGYSNRVVDVAEQIGALLTS
                     KETVSAS"
     gene            complement(17491..18114)
                     /locus_tag="NBH32_001180"
     CDS             complement(17491..18114)
                     /locus_tag="NBH32_001180"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCD1514797.1"
                     /translation="MPKVIGLTGGIASGKSTVSELLSVFGFKVVDADKAAREAVKKGS
                     KGLAQVREVFGDEAIDENGEMNRRYMGDLVFNHPEKRLELNAIIHPIVRDIMEEEKQE
                     YLKQGYNVIMDIPLLFENELENTVDEVWVVYTSESIQMDRLMQRNNLSLEDAKARVYS
                     QISIDKKSRMADHVIDNLGDKLELKQNLERLLEEEGYIEKPNYGEED"
     gene            complement(18130..19002)
                     /gene="mutM"
                     /locus_tag="NBH32_001181"
     CDS             complement(18130..19002)
                     /gene="mutM"
                     /locus_tag="NBH32_001181"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCD1514798.1"
                     /translation="MPELPEVEHVKRGIEPYVINQKIEHVKFSDKVIEGKAQGKETII
                     KGIELDTFKTLSEGYTITNVERRSKYIVFQLDNKREQRTLISHLGMAGGFFIVDELED
                     IMIPNYRKHWHVIFELSNDKKLIYSDIRRFGEIRNVASVASYPSFLEIAPEPFSNEAL
                     TYYLNRIHQQSNKNKPIKQVILDHKVIAGCGNIYACEALFRAGVLPDKKVKDLTHQQQ
                     EMVFYYVREVLEEGIKYGGTSISDYRHADGKTGEMQLHLNVYKQPVCKVCGSQIETKI
                     IATRNSHYCPVCQK"
     gene            complement(19018..21648)
                     /gene="polA"
                     /locus_tag="NBH32_001182"
     CDS             complement(19018..21648)
                     /gene="polA"
                     /locus_tag="NBH32_001182"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCD1514799.1"
                     /translation="MNKLVLIDGNSLSFRAFYALPLLSNKAGIHTNAVYGFAMLLEKI
                     LKEEKPNHFLVAFDAGKTTFRHEKYSEYKGGRQKTPPELSEQFPYIRQLLDAYHIKRY
                     ELDNYEADDIIGTLSKEADKAGFQTIIITGDRDLTQLATDNVTIYYTKKGVTDVDHYT
                     PDFIAEKYNGLTPNQIIDMKGLMGDTSDNIPGVAGVGEKTAIKLLNQFDTVEGVYEHL
                     DEISGKKLKEKLQNSKEDALMSKELATINVDSPIEVKLEDTLMTHQDEQQEKIELFKK
                     LEFKQLLADIDQSASVEDAIEKTIEIETSFDNVDFTSLKEAAIHFELDGGNYLRNNIL
                     KFSLFTGEKHIVINADDINNYAELVSWLENPNTKKVVYDAKKTYVASHRLGIDIQNIS
                     FDIMLASYIIDPSRTISDVQSVVSLYGQSFVKDDVSIYGKGKKFKVPEDDVLNPYVAS
                     ITDAIYFAKPNMDKQLEEYNQVELLADLELPLAKILSEMEEIGIFTDVHDLEEMEKEI
                     QEKLDVLIRNIHDAAGEDFNINSPKQLGVVLFETLQLPVIKKTKTGYSTAVDVLEQLQ
                     GEHPIIDYILEYRQLSKLQSTYVEGLQKVISDDQRIHTRFNQTLAQTGRLSSVDPNLQ
                     NIPVRLEEGRKIRKAFKPTSKDSVILSADYSQIELRVLAHITQDESMKEAFINGDDIH
                     TATAMKVFGVEADQVDSLMRRQAKAVNFGIVYGISDYGLSQSLGITRKKAKAFIDDYL
                     ASFPGVKQYMSDIVKDAKALGYVETLLHRRRYIPDITSRNFNLRGFAERTAMNTPIQG
                     SAADIIKLAMVKFAQKMKETTYQAKLLLQVHDELIFEVPKSEVDSFSEFVEEIMENAL
                     QLDVPLKVDSSYGATWYDAK"
     gene            complement(21942..>22942)
                     /locus_tag="NBH32_001183"
     CDS             complement(21942..>22942)
                     /locus_tag="NBH32_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514800.1"
                     /translation="CFFGQLFMENVTLFHCIILILAFLYEFTANRTLNYKLLFSFMIA
                     TIGTIIMFSNPNYRKILFEGSEYQQVSNNQGIFSKFAEMISTSLPYGVIFSQIIILSM
                     IAALIIYLLLRSDRYVHLTILKRRIIMIGFITLPLYYLLFYNQFLLNKNTDIGLVGFV
                     NVIVCGYFAFSIFVGIYLSINDRKTQITLYSLLIAICVSASTLVIVTPIEPGNFLIVY
                     TIHVIILIILLKEFRKYKSINVNFIKVASLVLAMIYLSAFIYVHYEHEKRVQLLKQQI
                     KDHPRQEVYTVESLPFEHYMHHPSPINKNYQEWFNNYEELPKMTKVKYIPFGSDDS"
BASE COUNT         7095 a         4334 c         3095 g         8418 t
ORIGIN      
        1 gaattcaacc atttgttgat tctaagtaaa atataacttc catataatac tgtaataatt
       61 gaagagagta ttaccttcgg gtcaatgaat atacgttcac caactgaaat tacaccccac
      121 tgtgtaccta aaataatact aaatatgaga attatccacc cacttaacgt tgagtaaaac
      181 acaattgatt caagtgtagc aacgctacct attctaaagt atttttgatc aaaacgcttt
      241 tccttcaaat tacggtattg catgatatac agtaatgcat tgacaaaagc taaggcaaag
      301 aagacataac ttaacacagc tagaccgata tggactaaca gtaactcgtc tacaacagca
      361 attttctgaa ccttattagt ataatgtgtc ggttgaaatg tattcatccc taaaagtgtt
      421 agccctatta aattcaagaa aaacactgag aaattcaaaa ctttgataag gttaagaatt
      481 aaagagattg aaataatcag ccaacttaaa gtatagaata catctgaaat agaccctaat
      541 ggaatatgtc tagtttgtat aataaaaata gataaagaga ttgtttgtaa aacccaaaca
      601 atccccaata aatatatgcc taaacttcta atcttatgac ttttttggac aaaatcataa
      661 aaatagcaaa tgatactgat taagtatatt aataatataa tttcattgaa tcgaataaac
      721 aggttttctt gcatattatc accatattaa tatgcttatt caaaactaaa gatacgtcgt
      781 gctgaaattt ccttgacctt gctttctttt tgttgcttcg cttgctcatg aggacattca
      841 gcttcgatat caaatatatt ttgaaatagc tctaattttt cattactttt cttatcacta
      901 cttaattctt tggcctgttt aataggatct ttcaacattt gattgatgat actttttgta
      961 tgttttgata taattttacg ttctctttcg cttaaccctg gcaatttacg atcaatactg
     1021 tccatcgttt ctgcttgaat tgtcatagct ttttcacgta aagctctaat cactggaaca
     1081 acacccaaca tactaatcca ctcattgtgt gcatgtattt ctgcaggaat ttgttccgaa
     1141 attgttgcag ccgctaattg tcgctcacgt aagtttgcat caactaaacc ttttaagtca
     1201 tcaacatcat aattaaagat gtttgtgatg gcactaatac caggttcaat atctcgagga
     1261 actgcaatat caatcaatac tagtgaatct tgctttctat tttctgcaat tctttctatc
     1321 atttcatttg taatgatata agattgtgca ctcgttgaac taatcacaat atctgcactt
     1381 tcaagtaaat ttggtaatga tgatagttca tcatatttca cttgatgctt tgctgctaat
     1441 ttcatagcat tttcaattgt tctatttact actgtaatat cagtaattcc agaaccaaga
     1501 agatttaata gtgataattc actcatttcc cctgcaccaa taatgatagc ttgcttactt
     1561 ttcaatttgc caaatacttt tttcgccaac tcgaccgcag cataagacac acttacagca
     1621 ttatcagcta tatctgtttc attatgtgct ctttttgcaa aagtaattgc ctgtttaaat
     1681 agatgattaa aaattgttcc tgtcgtacct gtgctttgcg ctaagaaaaa tgcatctctt
     1741 atttgaccta aaatttgagt ttctccaagt acgattgaat ctaaaccaga agtgacacgc
     1801 aataaatgtt ctactgcttc gtcccccact tttacttctg acattgcttt aatatcatct
     1861 acttcaaatc caaatgcacg agctagaaat cgttgaatat agtaacgacc tgtgtgaatt
     1921 tgatcaacaa cagcatatac ttcagttcga ttacatgttg ataatatgac attttctaaa
     1981 atagatttag tttcatataa atcttcatgg gcaattcgta aggcatcatc tctaaaagca
     2041 acttgctctc ttagtgcaac atcagctgtg cgatgattta tactaattgc aataaaatgc
     2101 atttgaaacg cccccatata tactaacaat acaaatatta taacataatt taaaatccaa
     2161 ttaagtacga tgcttaaata aattataatc tttctaattt acttttgagt ttttgacaaa
     2221 atgatgaacg ttgaaaaatg ttatcaattg tgtactaatt aaaagtttcg tgcttacatt
     2281 atttttaaaa tttttgtata agttttgtcg tacaaaaact atgaaatata cggttcaatt
     2341 aaattccata tttgttgttg tttattattt ttaattgatg aataacttac aattgtatcg
     2401 tctgggtcca tatctaattg tgtcttaata tttttaatat gcttttgaac tttacctttt
     2461 ggaattttgt cttctttagt gcatataact aaagtaggaa tatcaaaatg cttcaaataa
     2521 ttgtacatta agatatcatc ttgtgttgga tcatgtctta aatcaactaa ttgaataact
     2581 aattgcaaat tctctctctt agttatatat tcctcaatca ttttcccaaa tttttcacgt
     2641 tgtgttttac ttactttagc atatccatac cctggaacat ccacaaaaat aagttgttca
     2701 tctatattat aaaaatttaa cgtttgcgtt ttgccgggtt gctgtgatgt acgtgccata
     2761 ttttttctgc caatcatact attgataaat gtagacttac ctacattaga tcgaccgctc
     2821 agtgcaactt cagacaattc tgtttctgga tattgttctt cttttactgc actaatgatt
     2881 aattctatat tattaggatt aactttcatt ttatatcctc acttttttat attctctttc
     2941 aactctacca ttatataata tttgaacctt tcggtataca ttataaaaaa gctccgatca
     3001 aagttaaact atttgagcgt caactttgat tggagctttt cacttttata acacatcaat
     3061 gattaagctg atgttttact attattaatt aaattacctt ctgcgtcgta tagttctggt
     3121 tcagtttctt cattaattgt ttgtgctgta ataactacct tcgttacatt ttcgttagaa
     3181 ggcacatcaa acataatatc gattaacgat tcttctatga ttgaacgtaa accacgcgca
     3241 cctgtttttc tttcaattgc tttttcacta attgctgata aagcttcttc agtgaactct
     3301 aaatccacat catctaattc cagcatttta gtatattgtt tcacaagtgc atttttaggt
     3361 tgcgttaaga tgtttttcaa cgcagttaca tctaatgttt ctaaattagc tacaattggc
     3421 acacgtccga taaattcagg aatcaaacca taggcttgca aatcttctgg gcgaatttgt
     3481 gctaataatg cttgttcgtc atatttatca gcttcattgc ttgagaaacc aataactttt
     3541 tcaccaagac ggcgcttaat cacttcttca ataccatcaa aggcaccacc aagaataaat
     3601 aagatatttg ttgtatcaat ttgaatcatt tcttggtttg gatgtttgcg tccaccttgt
     3661 ggcggaacac tcgcagttgt gccttctaag attttaagca atgcttgttg aacaccttca
     3721 cctgaaacgt cacgtgttat agatgtgttt tcagatttac gtgcaatttt atcaatttca
     3781 tctacataaa taataccttt ttcggcttta tcaatgtcaa agtcagctgc ttgaattaat
     3841 ctcaacaaga tattttcaac atcatcgcct acatagccag cttcagttaa acttgtcgca
     3901 tctgcaattg caaatggtac attcaacgtc ttggctaagg tttgagctaa taatgtttta
     3961 ccactacctg ttggcccaat taaggcaatg ttactttttt gtaactcaac atcatcttct
     4021 tttggtccta attgttgaat acgcttatag tggttataaa cagccacagc taaagatttt
     4081 ttagcttttt cttgaccaat aacatattcg tttaaatgat ccataatttc tttaggagta
     4141 ggtaattctg taatcgcttc agaagtattt tgagctaatt cttcttcgac gatttctgag
     4201 cataattcaa tacactcatt acaaatatat acaccacttc ctgctacaag tttttttact
     4261 tgatcttggt cttttccgca gaaagagcat ttcaaatttt cttcatcttc attgaattta
     4321 aacattcttt ttacacccct attcgttaaa gactatacta gattggatat tacaatgcaa
     4381 catattaaca tacaaacttt ttgcttaaag aatagtagca gatacataag ctatgcatct
     4441 gctactcatt tttgtaattt aatatttaat gaagattaat cttctttagt tccttcaacg
     4501 aactttgcgt tatctcttaa taaatcgata actttttgga tacgaacatc atttttaatg
     4561 atatcagtat tacctaaagt atttttgata tcttcaactg agatattaaa ttgtttactc
     4621 attttttcta attctttatc gatatcttca tcagtagctt cgattttttc agcttcagcg
     4681 atcgcagtta aagttaagtt agttttaaca cgttgttctg catcgtcttt catttgctct
     4741 cttaattgag attcatcttg acctgagatt tggaagtacg tttgtaaatc taaaccttgt
     4801 tgttgaattc tttgtgcaaa ttcagacacc atacgatcta attcagtatt aaccattgct
     4861 tcaggaatat cgattgttgt attatcagta gcttttgtaa tcgcttcttc tttttcaaca
     4921 ttttcagcat ctgtagcttt ttgttcagct aaacgtttac gtaagttttc tttatactcg
     4981 tctactgtat ttgcttccgc atctaattca ttagcaattt catctgttaa ttctggaact
     5041 tctttaaatt taatttcgtt aacttttgtt ttaaaagttg cttctttacc agctaattct
     5101 tcagcatggt attcttctgg gaatgttacg acaacatctt tttcttcgtc aactttcata
     5161 ccttctaatt gctcttcgaa accaggaatg aatgaacctg aaccgatttc taaatcgtaa
     5221 ccttcagctt gtccaccttc gaattcttct ccgtcaactg aaccactaaa gtcgatgtta
     5281 actgtgtcgc cattttcaac aacaccatct tctttaacta ccatttcagc taaatgtcct
     5341 aagctgtggt caatcgcttc ttgtaactca tcatcagata attcagtttc ttgtttttca
     5401 atttcaagac ctttatagtc tcctaattta acttctggct caactgtaac tgttgcttca
     5461 aaaataaaat ctttaccttt ttcaatttga gtaacactta cttctggttg tgcaactggt
     5521 ttaatatcag tttcgtcaat tgcttcacca taagcatctg gtaataaaat gtcgatagca
     5581 tcttgatata atgcttctac accaaagcgt tgttcgaaaa ttggacgtgg tactttacct
     5641 ttacggaatc caggtacgtt aatttgttta accacttttt tgaatgcttg atctaacgct
     5701 ttgtttactt tttctgcagg aacagtaaca gttaataaac cttcgttacc ttcctttttt
     5761 tcccaagttg ctgtcatgta tatatacctc catgattaac taatttattt tttcaacttc
     5821 cctattatat cataggtcta ttccctatac aaacattgaa atcacaacgt ttatatattt
     5881 gtaaatcaac ttttttcgtc aaaactatat taaaatgctg agtattattt tgcaacaatt
     5941 taactattat tattcaaatc taattgctga ataaaattaa atgcatcgat actttgtaat
     6001 tcacatttaa gtcctaacat atttttgaaa taacattcgt atgcattaat ccatttattc
     6061 gtttcaaaca aagtttcaat atcaattgga taaatcatga tagagtgatt attcataata
     6121 tggtgtgctt cttcagcgat atgtaaagca ccatcattta atgtttccat aacgttaggt
     6181 ataacttttt ctttcagcgt tgtatgttct agcccattta aattagctgg tacaaaagtt
     6241 acatccatac catacttttc aattgtcagt tcttgtgtac aatttgcaaa ccttaaatac
     6301 tcaatcatta aactaattag gttataactg tacgtattag attttaatat atataatacc
     6361 gtttcttgaa attgaaaatg accaatgtct attaacttca aaatcaagtg cgtctgttcc
     6421 ctcattgata acgtatcaaa atcagttaat gactgagtca atcgtttttc atcttcaatt
     6481 aattttgact tagcaaattc tttaagagga tgtaatgcca ttcttgtttt gtgatctttg
     6541 acttcatcaa taatttgatg aattacttct accgcttcaa aatattgtcc caaaccaatc
     6601 aaacttttca cgtaataaat catcaatgca tcatattgtt gtatgccagt tttcaataag
     6661 acaattgttt cttctcttaa ttcaagaaat gaccccgtct catataacat acggcatttc
     6721 atcattgcta tttcttcagt gagttcaaat tgacgttcat attgcacaat atattcatac
     6781 atcaggtcat agttttggtc ttgttcagca cgttttatat ctttatataa ctttatcgaa
     6841 gagttaggaa actgaataat atctgacata tatgtcacct cgtttgatta taaatcttga
     6901 aggattaatg cgctccacgt ttcgaaatca tcatatgact ctatgttgcc tttttcaacc
     6961 cattttattc gtaaagtatc tgtttcttta gcttctacat cattcgtact tacagtgatt
     7021 ttaaatacaa caccaatatg taccttgccc acttcattat tatcgtcatt aataaaaccg
     7081 atatactcca tattttgtga atcttgctca cttaaaccta cttcttcttc taattctctc
     7141 tgagcattaa ctctcaatac ttcgttaata gattctgctc ctggaacatc attcatatga
     7201 ccgcctacac ctattgaaga ttgtccatgc aatcgagctt ctccaccgcc agataatcgt
     7261 tcatacacta atatctcgcc atgctcattt tcaagtaaac aataagaaat gagttgttta
     7321 tatgatggat cctcttccat atcgccgcgt cgcttaactt catattgact tagcgcatca
     7381 aaaatatttt gaccttctgg tttattctta tttaaaaacc cattaaaagt attcttttca
     7441 ttattaaaaa taatttctct aggtactaca atgatttgtt catcaaattt agacatcgta
     7501 taaaactcct cactttaatg tttgttctct taaatgcatt ttagcaaata tgtcatattt
     7561 tagcgattaa atgtggcatt ttgctattta aaaaataaaa aagagatgac aatgccatct
     7621 cttttttata agtgttatta ttttaaagca tctttagctt tagttactaa ttgagcaaat
     7681 gctttttcat cagaaattgc aatttctgat aacattttac ggttaatgtc gataccagct
     7741 tttttcaaac cgttcattaa acgtgagtag ctcatttcat gttgacgagc tgctgcgttg
     7801 atacgtgtaa tccataattt acggaagtca cgtttacgtt gacgacggtc acggaaagcg
     7861 tattgacctg atttcattac ttgttgctta gctactttgt ataatgtatg ttttgaaccg
     7921 aagtaacctt tagctaattt aatcgttttt ttacgacgcg ctcttgttac tgttccacct
     7981 ttaactcgtg gcataaaaaa ttcctcctta ggtaattcct accgatttct gtatttgttc
     8041 ttattttttg tatgctaata attgttttac acgtttcatg tcgctcttag acactaatct
     8101 agctttacgt aattgacgtt tttgtttagt gctcttgttt gcgaataagt gagatgtgaa
     8161 agctcttgaa cgttttaatt gacctgaagc agttctttta acacgtttag ctgctccgcg
     8221 gtgagttttc atttttggca taatataagt cctcctgtaa tagctttaat tatttttcag
     8281 ctgttggcgc taacatgata aacatttgac gcccgtccat tttaggtttt tgttcaactg
     8341 ttgctatatc tttgcattca tctgcatatt tttctagcac acgttgacca atttccttat
     8401 gcgtaatggc acgccctctg aaacgaatag atactttaca tttatcgcct ttagttaaga
     8461 atttacgtcc gtttttcaac ttagtttgga aatcatgttc ctcaattgtt ggacttaaac
     8521 gaatttcttt aacattgata attttttgtt tctttttcat ttctttttct tttttctgtt
     8581 gttcgaattt gaatttaccg taatccataa ttcttgcaac tggtggtttc gcattcggtg
     8641 caacgaccac taagtctaaa tctacacgtt cagccatttc taaagcttct cgctttgatt
     8701 taacaccaat ttgttcacca tcttgaccga ttaaacgtaa ttcttttgca cgaattttgt
     8761 cattgatttg agtttgatct tttgctatgg ttgacacctc caaaattttt acgaaatttg
     8821 caccaagcaa aaaggaagag caggtataaa atacccgctc ttccttatac acagatatgt
     8881 gtaatgtgat taacctgcca actgctttat gcgtcgctac aggtgagaag cgggtgcttc
     8941 tacttggttc gtttcgtatt caacgttgtt aatcatatca acaattcaca tttaagtcaa
     9001 cactataact gtaattattt ttattttaac cttttatttc atccattgac acgtcttgac
     9061 gtaaatctac ttgttctaat ggaatttttt tcgttttata tcgaagctta tgataaataa
     9121 agaatgctaa aaatactgga attcccatat acgtaattaa gaagcgacta aaattaaaat
     9181 ctcctgtttt aataaagtca acatcttgac caataattac tacaatacat aaaaagccag
     9241 caaataatgg tccgaatggg aataatttag cagtatattt taatttagat ttgtcataat
     9301 tttgtttatc aaatgctctt ctaaatcgat aatgacttac tgctatacct acccaagcaa
     9361 taaaaccagt taaaccactt gctgcaacga tatattcgta tgcacctttt gtaaggcttt
     9421 gtaatacgaa aataatcact acaatgattg ctgtaacgag taacgacata tatggcacac
     9481 cgtttttatt tgttttacca aatgcttcaa acgctaattt atctttactc attgaatata
     9541 gcattcgagt tgaagcatac atacctgagt tacctgctga taacacagac gttaaaatga
     9601 ctgcattcat aaatgatgct gcaaacgcaa atccagcatt tttaaacact aatgtgaatg
     9661 gagacgttgc tacattatca ctaccaccca ttaatgcact actatcataa ggaattaaca
     9721 taccgataac aaaaatggct aaaatgtaaa ataataaaat tctccagaat acttgtttaa
     9781 ttgctttcgg cacagcacgt tcaggatttt ctgattcacc agccgtaata ccaattaact
     9841 cagtaccttg gaatgagaaa ccagcgatta agaatacacc tagaattgat aacaaacttc
     9901 cgcctagatt gccaccaaga ataggacctt cacctttatt aaatatttcg aatcctacaa
     9961 catgaccacc catgattccg acaatcgtta ataaaccaat tgcaatgaaa acaataactg
    10021 taaccacttt tatcaatgcc aaccagtatt cactttcacc atagacgcga actgataacg
    10081 aattcagact aaaaattata actaagaaca acgcactcca tgcccaagcg ggtatgcctt
    10141 gcaatggtgt ccaatattga atgacttgtg ctgcaatcgt aatatctgct gctacagtta
    10201 ctacccagtt aaaccaatag ttccagccaa gcgcaaatcc taaagatgga tcaacaaatc
    10261 ttgtagcata tgtactaaat gaacctgata ctggcaaata cgtagccatt tcgccaagtg
    10321 acgtcattaa gaaaaatacc attattccga taattgcgta tcctattaat gcacccaaag
    10381 cacctgcatc atgaattgct ccaccagaag ttacaaataa acctgtacca atacaacccc
    10441 caatcgcaat catagaaata tgacgatctt taagtcccct tttgacaaca ttgttacttt
    10501 cattttgaac ttttgacata taagctccct ttccatgttt tagaggccaa gaaataaata
    10561 acctttattc actttcatac ccttttttac agaacatagt gatattaaaa gtaaaaaagt
    10621 ggctacatac ctttaagata tgcaaccact ttggcaatta tctatataag gtagcacatc
    10681 acattctgtg acagtacagt tcctattcga aaactgtccc aatagatata atttatggtt
    10741 tatatctatt tcggcatctt tacctttcac ttgttcaact tatgtaccat aaatacttct
    10801 gacaagttac taattaaaca tgcaacctct aactcaattt aatattttaa ctaacttgta
    10861 atatacagga ttcatcacgc ataatcaacc ctgtgaaact tgatacgcaa taaaaatttc
    10921 aaagcatttt attgcgacaa ctgtctatct atgttttttt aaacgaattt catcaactag
    10981 attccagata aattcatctt tttcaactgt ttcttggtct tgcgatccat attgacgcac
    11041 attcacttga ttattttcaa cttccttatc cccaactacg atttgataag gtattttttg
    11101 catttgagct tctctaattt tataacccat tttttcatta cggtcatcaa tacttacacg
    11161 aacgccttga gatttcaatt catcttgtaa ttggcgcgca taatcataat gtaaatcaac
    11221 gttaactgga atgatttgaa cttgttttgg cgctaaccaa gttgggaatg cacctttagt
    11281 ttcttcagtt aaaaatgcta caaatcgttc cattgttgat acaacaccac gatgaataac
    11341 aactggacga tgatgttcac catcttgacc aatataagtt aaatcaaaac gttctggtaa
    11401 taagaaatca agttgtgctg ttgataatgt ctcttcttta cccatcgctg ttttaacttg
    11461 aacatctagt ttcggaccat agaatgccgc ttcaccaatc gcttcttcgt acgataagcc
    11521 aagctcatcc gctgcctctt taagcatatt ttcagcttta ttccacatat catcatcatc
    11581 aaagtacttt tctttatctt cagggtctct ataacttaat ctaaagctat aatcctcgaa
    11641 accaaagtct ttatacacat caataatcat gtttacaacg cgtttgaatt cttctttaat
    11701 ttgatcaggt cgaacaaaga tatgtgaatc atttaaagtc ataccacgaa cacgttgtaa
    11761 tcctgataca gcaccacttg cttcatatct atgcatcgtt cctagctcag cgatacggat
    11821 aggtaattca cgatatgaat gtggtttatt cgcataaatc atcatatgat gtggacagtt
    11881 cattggacgt aataccatag attctgtttc atctaactgc attggtggga acatatcttc
    11941 ttgatagtga tcccagtgac cagatgtttt gtataaatca acattagcaa gtactggtgt
    12001 ataaacgtgg tcatatccca tgctaacttc tttatcaaca atgtaacgtt caatttcacg
    12061 tctaattgtt gcaccgttag gtaaccataa tggcaaacca gcaccaacta attggctatt
    12121 tgtgaatagt tctaactctt taccaatttt acgatgatca cgttctttac gctcttctaa
    12181 catttgtaaa tgtgctttta attctttttt atcaaagaaa gcagtaccgt atatacgttg
    12241 taacattttg ttgttactat ctccacgcca gtatgcacct gctgtagata atagtttaaa
    12301 ctctttaatt ttagctgttg atggaacgtg aacgccacga cataaatcag taaaatcacc
    12361 ttgactatat aatgttacat tttcatcttc aggaatcgcg tcgattaatt ctaatttgta
    12421 ttcatcattg ctgaataact ctttcgcttc atctcgtgaa accacttttc gttcgatttt
    12481 catattttcg ttaacgattt gtttcattgt tttttcaatt tgttcaaagt catcagatga
    12541 gatgttttgg tcaatgtcga agtcatagta gaatccacct tctattacag gacctacacc
    12601 aaatttaaca ttaccatata accttttaat cgcgtgtgcc attaaatgtg cagtagaatg
    12661 acgtaatacc tctaacgctt cttcactacc tggtgtcaca atttcaattg atccatcagt
    12721 ttcaagcggt ttagttaaat ctacaagttg cccgttaaat ttgccggcaa cagctttttt
    12781 acgtaatcca ggactaattg attgtgctat atcttcagta gtagtacctt tatcaaacgc
    12841 ctttttatta ccatctggaa attgaatatt aatttgttcc atgttaaacc ctcctatttg
    12901 ctttataaat acttttatct gatctcgtta aaaaattgaa caacaatcat cgttttaaca
    12961 ttaaacctat taacaataaa cagggaattt atgaaaccaa cgaacatttt tgtctgaaaa
    13021 aaacaaaaaa tcccgcccct atacaaggga cgagatcgtc gtggtaccac cctagttatt
    13081 taatacaatt tatgtattaa atatctctgc ttaagataac ggtcttgatc cgggtattca
    13141 ttacaaatac cataaatgaa gtagtaatca cctaatttat taaccatatt ctcatcaaac
    13201 aatggctttc tgtgtaatat tgattagatc atcttgtctt caatcattta aacgatttag
    13261 atttgtattc attatacacc aatcatttta aaattcaatt gtttctgaaa ttttctcctg
    13321 ataaaaagta tggtgttgac aaagatttga cacgttcaat aatacgtgct gctttagtct
    13381 tctcttcacc atcacgagtc atcgctaaat gatgttccaa ttcactatag tcaaaattag
    13441 aactaaagaa tgttggtaat tcatgaacca ttcgataatg tagcaaaggt ccaattacct
    13501 catctctcac ccatggagtc acttcttcag ccccaatatc atcaagcatt aaaatgtttg
    13561 cttctcttac gcgatgtaat ttcttttcaa aagaaccatc tttaaagcca ccttttaatg
    13621 ttctaataaa ttccggtaaa taaataattg tcgaacgtac cttcttagat ttgagctgat
    13681 tcgcaattgc acctagaata aaagatttac ctgtcccaaa tggaccataa aggtaaaggc
    13741 ctttcacttg ttccccatta gttattgctg tacaaatatc atctgctgcc atagctacat
    13801 caagacggtc tcgatgattc atataaatat ctttcaattt ggcatttaaa gtatctcgtt
    13861 gcatatgatg agatgtaatt agctcagctt caaagcgttc ttcgtcgtac ttgattttac
    13921 atgggcattg taaatagcgt attttaattc ggttattatc aacatataac tcaggcacat
    13981 gcccctttac gaaatttgga caatcagcaa atttatgacc gtcataatgt ttttgttgat
    14041 ctttatactc ttgtaacaca tttaagtctt catcaatcat agcattcgtt aattcagctc
    14101 gatgcgcttc caaaaattgc ttaacatctg ggtcattgat tacttctttt tttatctttt
    14161 ctattctttt ttcaaagtcc tgcgacgtgt taattatact tttaaattgc ttcattattg
    14221 actgtcctcc tcccattttt tagataattt atctagaaat gcttgtcgat cttgctctaa
    14281 ttgttgatca tctacgctat tatctttagc cgaatcttct tcactaggtt tatctctatt
    14341 ttctaaccat ttaggtgttt tttcttttga aatacgatta cgctgcccat agtatgaacc
    14401 acgcttttgg taatttccgc tagaaccctc atttttaggt tgattaactt ttttagcgta
    14461 attatatgct tctttagctg tcttaatacc ttttttcttc caatttgatg ctatttccaa
    14521 aatatacgct ttaggaagtt tcatatcttc ttttaacatg acaaattgca acaaaatatt
    14581 aatgacgcca aaagacattt tttcacgttc aattaattct tcaaccattg tcttttgcga
    14641 tatagttggt tctgattcag accaagaagc taacatatca attggactcg tttgttcaag
    14701 taactcaaac cattcatcac tttgtggctt tggattcact tctgaagatt tgcccgtcga
    14761 agatgatgta acaggagatt tcacctgtaa tttaggcatt tgattttcgt gttccattaa
    14821 gtaatacgag cgtgcttgtt tacgcatttc ttcaaaggat aactgttgtc cacttgtaat
    14881 tgaatttaaa ataacatgct tcatgccatc tgctgttaaa ccatataaag tcgcgagttg
    14941 tgtaattaaa cgctttgcat ctttggtaac aatgtcttga ctaataaaat gtttacctaa
    15001 catttgtctc aacatttcaa agtcaaaaga ttcatttgat aaatcgatac cttggtacgg
    15061 ttcattaatc ggaatatcac ttgtatcgat atctattttt gtagacggca ctttaaaaac
    15121 atcagtaaat tgtcttgtta cctgtttaaa ttcactcaaa tcaatttgtt gatactcaaa
    15181 gtatttcttc aactcatgaa atcgacgatg ctcgacttca ctatataaaa agattgacaa
    15241 cattggatca ttaaaaaata aatgtgctga aggcggttga attaattggt aaacaaattg
    15301 tgtttcttgt tcatcatgtt tgacaaacgc ctttaacaat ccaatcgctt caagtaagtc
    15361 catttgttgt ctaaactcta gtaaattaat tttaagttca ttcataaaaa tataatgaga
    15421 aagaatcaat gtttcattat gactttcttt aacgaattgt gtcataaaat ggtataaacc
    15481 cactgcttgt gttccaatta gcggtgtata cagtcgattc aatacctcta aatgattcgt
    15541 atttaaatca aagtgttgca taactttgaa ttgatccttt ggtcttaagc cgaattcgaa
    15601 ggcttgtcgt cccatttaag catcactccg tttgttttcg cttaaaatcc cttgcatcga
    15661 tgctaacaat tgatcaacat ctttaaattc tttatagact gatgcaaatc taacatatga
    15721 aacttgatca acatgcatta acaagttcat aacgtgttca cctatatctc gtgaagacac
    15781 ttccgtatga ccttcatctc gtaattgcca ttcaaccttg ttagttatgt cttcaagttg
    15841 ttgatatcta actggtcgtt tctcacaaga acgcacaagt ccattaagta tcttttctct
    15901 tgaaaactgc tctcttgtgc catctttttt cacaactata agctgactaa cttcgatatg
    15961 ttcaaatgta gtgaaacgtg ttccacaatt ttcacattct cttcgtcttc gaatggcatt
    16021 taattcatcg gcatgccttg aatctacaac tttagattgt gtagaattac atttcgggca
    16081 tttcattaca tcaccctctt tattttgatt atgcctaatt atactataaa tctagagatg
    16141 aaaaaagaat ccctcaattt aattcattta accaaataat gaaacaataa aaaacattat
    16201 atcgttactt attaagtaat atgcatgaca atattattgt attaaaaata aaaaacctaa
    16261 ctccgaagtc agagtcaggc tattaaataa ttgtattaac ttgcacttac agtttctttt
    16321 gatgtcaaaa gtgctccaat ttgctcagca acatctacaa ctctatttga ataaccccat
    16381 tcattatcat accaagcaat aacttttact ttattccctg acatgaccat tgttgatttt
    16441 gcatcaataa tagctgaatg tggattagta ttgaaatcaa cagacactag tggttgatgt
    16501 tcgacttcta tgataccttc taaaccagca ttttcaaaag cttggtttac ttcttctgcc
    16561 gttacttctt tttctaaatc aacaactaaa tcaacgagcg atacattctt tgttggtaca
    16621 cgtaatgcca tgccgtgtaa tttaccttct aattctggca atacttcttt taaagctttc
    16681 gccgcaccag tagaagtagg aataatgctt tcattacatg agcgtgcacg tcttaaatct
    16741 ttatgtggat tatcaatatt tttttggtca tttgtaatag cgtgaacagt agtcattaaa
    16801 ccattaacta ttccaaactg attatttaaa acttttgcaa ctggaccaat gcaattagta
    16861 gtacatgaag cattactaaa aatgtcaaat gcttctatat ctaattggtc atcatttacg
    16921 cctttaacta ccatttgaac atgtccacct tttgaaggac cagttaacaa tacttttttg
    16981 gcacctgctt taatatgtgc gatggcttta tcaccatgat taaatttacc agttgcatct
    17041 atagcaatat cgatatctaa ttctttccat ggcaagtttt caggattgcg atcagcaacc
    17101 aatttaattt tatgatcacc aacttgcaat ccgttttcaa ttggttctac ttttagatta
    17161 tattttccat gtgttgtatc gtaattgatt aaatgtgcaa ttgtttcggg tggataacta
    17221 gcattaatcg ctactacatt taaattttta ttttgtaatg caatacgtaa taccattctt
    17281 ccaattctac ccataccatt aattgcaata ttcgttgaca ttaaaagcac cccttgattt
    17341 ttatgtgtta tactttatgg aattagtata gcatattata tgaaatttga aagcgttttc
    17401 cgtcgattta tttatctttt ttataaaaaa tagtcattac ttacatacat caacccgtac
    17461 gtaaagtaat gacttattta tagtgtaata ttaatcttct tctccgtaat tcggcttttc
    17521 aatataacct tcttcttcta acaatctctc aaggttttgt tttaattcaa gtttatcccc
    17581 taaattatcg ataacatgat cggccattcg gcttttttta tcaatagaaa tttggctata
    17641 gacacgagct ttcgcatctt ctaatgacaa attattacgt tgcattaaac gatccatttg
    17701 tatactttca gaagtgtata caacccacac ttcgtctact gtattttcca attcattttc
    17761 aaataataat ggaatatcca tgattacatt atatccttgt tttaaatatt cttgcttttc
    17821 ttcttccata atatctcgca cgataggatg tatgatagca tttaattcta agcgtttttc
    17881 tggatggtta aacactaaat cacccatata acgacgattc atctcgccat tttcatcaat
    17941 tgcttcatca ccaaagactt ctcgtacttg agctaaacct ttactcccct ttttaacagc
    18001 ttccctggct gctttatcag catctactac tttaaaaccg aatacggata agagttctga
    18061 tactgttgat tttcctgagg cgattccacc tgttagacca ataactttcg gcataatttc
    18121 actctttctt tatttttgac atactggaca ataatgacta tttcttgtcg cgatgatttt
    18181 tgtttcaatt tgacttccac acactttgca taccggctgc ttatatacat taagatgcaa
    18241 ttgcatctca ccagtttttc catcagcatg acgataatct gaaatacttg taccgccata
    18301 tttaatacct tcttctagta cttctctaac ataataaaaa accatttctt gttgttggtg
    18361 tgttaagtct tttacttttt tatctggtaa aacacctgca cgaaacaacg cttcacatgc
    18421 gtaaatattt ccacaacctg cgattacttt atgatccaaa atcacttgtt tgattggttt
    18481 attcttatta gactgttgat gaattcgatt taaataatac gtcaatgctt catttgaaaa
    18541 aggttcaggc gctatttcta aaaatgaagg gtaagatgct acagacgcaa catttctaat
    18601 ttctccaaaa cgccgtatat ctgaataaat taactttttg tcatttgaca actcaaaaat
    18661 aacatgccaa tgcttacgat aattaggtat cataatatct tcaagttcat ctacaatgaa
    18721 aaaaccaccc gccataccta aatgactaat taatgtacgt tgttctcgtt tattatctag
    18781 ctgaaaaacg atatatttac ttcttcgttc tacatttgta atggtatagc cttccgataa
    18841 agttttaaaa gtatctaatt caattccttt tataattgtt tccttgcctt gagctttacc
    18901 ttcaattact ttatccgaaa atttaacgtg ttcaattttt tgatttataa cgtagggttc
    18961 aattcctctt tttacatgtt ctacttctgg taattcgggc ataccattaa cctcacttta
    19021 ttttgcatca taccaagttg caccataact tgagtctact tttaatggaa catctaattg
    19081 caatgcattt tccattatct cttctacaaa ttcactaaat gaatctactt ctgacttagg
    19141 tacttcaaaa attaattcat cgtgtacttg taataatagt ttagcttgat atgttgtctc
    19201 tttcattttt tgtgcaaatt taaccattgc cagtttaatg atatctgcag cactgccttg
    19261 tattggcgta ttcatagcag tacgttcagc aaagccgcgt aaattaaagt tacgactcgt
    19321 aatatcagga atatagcgtc gacgatgtag caatgtttcc acataaccta aagctttggc
    19381 atcttttaca atatcagaca tatattgttt tacacctggg aaactagcta aataatcatc
    19441 aatgaatgct tttgcttttt tacgagtaat acctaaactt tgacttaaac cataatcact
    19501 tatcccataa acaattccaa agttaaccgc ttttgcttga cgacgcatta aactatcgac
    19561 ttgatcagct tctacaccaa atactttcat agcagttgct gtatgaatat catcgccgtt
    19621 gataaatgct tctttcatac tctcatcttg tgtaatgtgt gctaatacac gcaattcaat
    19681 ttgagaataa tctgctgata atataacgct atcttttgaa gttggtttaa aggcttttct
    19741 tattttacgc ccttcttcaa gtctaaccgg aatattttgt aaattaggat ctacacttga
    19801 taaacgtcca gtttgcgcta aagtttggtt aaaacgtgta tggatacgtt gatcatcact
    19861 aattaccttt tgcaatcctt caacataagt agactgtaac ttagataatt gacgatattc
    19921 taaaatataa tcaatgatag gatgttcacc ttgcaattgc tctaagacat ctacagcagt
    19981 agaatatcct gttttcgtct ttttaataac aggtaattgt aatgtctcaa acaatacaac
    20041 acctaattgc ttaggagaat ttatattaaa atcttcacca gctgcatcat ggatatttcg
    20101 aatcaagacg tctaattttt cttgaatttc tttttccatt tcttctaaat catgaacatc
    20161 tgtaaatata ccaatttctt ccatttcact taaaatctta gctagcggta gctctaaatc
    20221 agctaagagt tctacctgat tgtattcttc taattgttta tccatatttg gtttcgcaaa
    20281 gtaaattgca tcagtaatag aagcaacata tggatttaaa acatcatctt caggtacctt
    20341 aaatttctta ccttttccat atatactcac atcgtctttc acaaaacttt gaccgtacaa
    20401 tgaaacaact gattgaacat cactaatcgt acgagatgga tcaattatat aactggccaa
    20461 cataatatcg aaagaaatat tttgaatatc aattcccaat ctatgtgatg ctacatatgt
    20521 ttttttagca tcatatacga cttttttcgt attcggattt tctaaccatg aaactagttc
    20581 ggcataatta tttatgtcat ccgcattaat tacaatatgt ttctcacctg taaataaaga
    20641 gaattttaaa atattatttc gcaaatagtt accaccgtct aattcgaaat ggatggccgc
    20701 ttctttcaat gaagtaaaat caacattatc aaaagacgtt tcaatttcaa ttgtcttttc
    20761 tattgcatct tcaacgcttg ctgattgatc aatgtcagcc aacaattgtt tgaattctaa
    20821 cttcttaaac aattcgattt tttcttgttg ttcatcttga tgagtcatta acgtatcttc
    20881 aagttttact tcaatcggac tatctacatt aatcgttgct aattctttac tcattaatgc
    20941 atcttctttg ctattttgaa gtttctcttt taacttttta cctgaaattt catctaaatg
    21001 ttcatagaca ccttctactg tgtcaaattg gtttagcaat tttattgctg ttttctctcc
    21061 aacacctgca acacctggta tattatcaga agtatctccc attaatcctt tcatatcaat
    21121 aatttgatta ggtgttaacc cgttgtattt ttccgcaata aagtcaggtg tataatgatc
    21181 aacatcagta acaccttttt tagtgtaata aatggttaca ttatccgttg caagttgtgt
    21241 taaatctcgg tctcccgtaa taataattgt ctgaaagccc gctttatctg cttctttact
    21301 taaagttccg ataatatcat ctgcctcata gttatctaat tcataacgtt taatatgata
    21361 tgcatctaat aattggcgta tataaggaaa ttgctcactt agttcaggcg gcgttttctg
    21421 gcgtccacct ttatattcac tatatttttc atgtctgaaa gtcgttttac ctgcatcaaa
    21481 cgctactaaa aaatgatttg gcttttcttc ttttaaaatc ttctctagta acattgcaaa
    21541 accatatact gcattggtat gaatgcctgc tttgtttgat aacaaaggta atgcataaaa
    21601 agctctaaaa cttaagctat taccatcgat taatactaat ttattcacaa ttttaacctc
    21661 cagaactaat ttatatatat tttagcatat tcgactgtga tgtcgtaatt aaagaaacta
    21721 tctcatagag tatttataca cgacctaatt tcaaataaaa caagtgacta tatagactaa
    21781 gctaaaaaat aaaaatgaca taatcatgat tgttcttgat tagcactcca taaagtcagg
    21841 ttaaaagtcc aacttttgag atgcacatta tcatttcatg attatgtcta ctttttaata
    21901 tttttatgct gctattttaa atttttatag gtaagaataa gttatgaatc gtcagaacca
    21961 aatggaatat atttaacttt ggtcattttc ggtaattctt cataattatt aaaccactct
    22021 tggtaatttt tattgattgg tgacggatga tgcatataat gttcaaacgg caatgattcc
    22081 actgtataaa cctcttgtcg tggatgatct ttaatttgtt gttttaataa ctgaactctt
    22141 ttttcatgtt cataatgaac ataaataaat gcactaagat atatcatagc taaaactagt
    22201 gatgcaactt taataaaatt aacattgatt gacttatatt tccgaaattc ttttaataaa
    22261 ataattaaaa tgattacatg tatcgtataa acaatcaaaa aattacctgg ttctattgga
    22321 gtaacaatga ctagtgtcga cgccgaaaca catattgcaa taagtaagga atataaagtg
    22381 atttgtgttt tacgatcatt aatagacaaa taaataccta caaatatcga aaacgcaaag
    22441 taaccacata caattacgtt cacaaaacca accaatccaa tatctgtatt tttatttaat
    22501 aaaaactgat tgtaaaatag taaataataa agcggtaaag tgataaatcc tatcataatg
    22561 atacgacgct ttaatattgt taaatgtaca taccgatcac ttctaagcag tagataaata
    22621 ataagcgctg caatcatact taaaattata atctgactga aaatgacacc atatggaagt
    22681 gacgttgata tcatttctgc aaattttgaa aagatacctt gattatttga aacttgttga
    22741 tattctgatc cttcaaataa aattttgcga taatttggat tggaaaacat aatgattgta
    22801 cctatagtag caatcataaa tgaaaacaat aatttataat taagtgttcg atttgcagta
    22861 aattcataca gaaaagctaa aattaaaatg atacagtgga ataatgtcac attctccata
    22921 aataattgac cgaagaagca ta
//
LOCUS       DAHHOJ010000036        22641 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.036, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000036 DAHHOJ010000000
VERSION     DAHHOJ010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22641
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.036"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..349
                     /locus_tag="NBH32_001184"
     CDS             <1..349
                     /locus_tag="NBH32_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1514801.1"
                     /translation="YDLTYTGYIVLMAIENDEKLNIKKLGERVFLDSGTLTPLLKKLE
                     KKDYVVRTREEKDERNLQISLTEQGKAIKSPLAEISVKVFNEFNISEREASDIVNNLR
                     NFVSKNFDNSDKK"
     gene            564..992
                     /locus_tag="NBH32_001185"
     CDS             564..992
                     /locus_tag="NBH32_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HCD1514802.1"
                     /translation="MILNFNQFENQNFFNGNPSDTFKDLGKQVFNYFSTPSFVTNIYE
                     TDELYYLEAELAGVNKEDISIDFNNNTLTIQATRNAKHKSEQLILDERNFESLMRQFD
                     FEAVDKQHITASFENGLLTITLPKIKPSNDTTSSTSIPIS"
     gene            complement(1100..2269)
                     /locus_tag="NBH32_001186"
     CDS             complement(1100..2269)
                     /locus_tag="NBH32_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000278551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NarK/NasA family nitrate transporter"
                     /protein_id="HCD1514803.1"
                     /translation="MYKTKGGFQLTLQTLSLVVGFMAWSIIAPLMPFIKQDVNVTEGQ
                     ISIILAIPVILGSVLRVPFGYLTNIVGAKWVFFTSFIVLLFPIFFLSQAQTPGMLMAS
                     GFFLGVGGAIFSVGVTSVPKYFPKEKVGLANGIYGMGNIGTAVSSFLAPPIAGIIGWQ
                     TTVRSYLIIIALFALIMFIFGDTQERKIKVPLMAQMKTLSKNYKLYYLSYWYFITFGA
                     FVAFGIFLPNYLVNHFGIDKVDAGIRSGVFIALATFLRPIGGILGDKFNAVKVLMIDF
                     VIMIIGAVILGISDHIALFTVGCLTISICAGIGNGLIFKLVPSYFSNEAGSANGIVSM
                     MGGLGGFFPPLVITYVANLTGSSHLAFIFLAVFGCIALFTMRHLYQKEYGSLKHS"
     gene            2639..3553
                     /locus_tag="NBH32_001187"
     CDS             2639..3553
                     /locus_tag="NBH32_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000276756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2268 domain-containing protein"
                     /protein_id="HCD1514804.1"
                     /translation="MYKINIIRSDSVYKKILKAPLNERDSIFTKEILVPFKKKFEVQQ
                     IPIHNDDKQTMSAIQLLDVFHKSPEELVETDQMSIQYLNNDFWINCEKYLKVAIDQFA
                     NYSISSQVSNYHFTVLLGDSQKPLMYLNKNRGGDGGIPGYIMIYLVPSTSTINSMKSL
                     IAHEVNHNMRYQYIDWDGGSLIELIIAEGLAENYVESLYGKAHIGPWVTNTNWSRDNV
                     KIKNTIYNHLHLKHIFESMPYLYGDDINKLQGRPIVGLSHAAGYACGYHLVKYFLQKS
                     NIPIEVATTLPAQKIINEVTEFWHTHTL"
     gene            3532..4272
                     /locus_tag="NBH32_001188"
     CDS             3532..4272
                     /locus_tag="NBH32_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1514805.1"
                     /translation="MAYTYTLKDIIEITGVTKRTLHYYDEIGLLVPDKNDKNYRVYKQ
                     QDLEKLQKILILKSFDFDIAKIKQYISYDNEQLRKLLSEQISKLDKKISDLQLIRRSV
                     CEFIKGHSLIDTSILNKTLQSQYDKEASIKYGHTKAYQSFIRRKDNLQSQDIRQKLST
                     IFNKFNHMSLSHYPIQDCNDIVTEWKSFMNTIADFDDETLCCIAKTYEDDTRFKDYFN
                     SYDNQNLASYISEAVNYFLSNVNKSDNF"
     gene            complement(4527..5180)
                     /gene="nreC"
                     /locus_tag="NBH32_001189"
     CDS             complement(4527..5180)
                     /gene="nreC"
                     /locus_tag="NBH32_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002464689.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation response
                     regulator NreC"
                     /protein_id="HCD1514806.1"
                     /translation="MKIVIADDHAVVRTGFSMILNYQNDMEVVATAADGVEAYQKVME
                     YKPDVLLMDLSMPPGESGLIATSKIADSFPETKILILTMFDDEEYLFHVLRNGAKGYI
                     LKNAPDEQLLLAIRTVYKGETYVDMKLTTSLVNEFVSNSNQDTANTSDPFKILSKREL
                     EILPLIAKGYGNKEIAEKLFVSVKTVEAHKTHIMTKLGLKSKPELVEYALKKKLLEF"
     gene            complement(5203..6237)
                     /gene="nreB"
                     /locus_tag="NBH32_001190"
     CDS             complement(5203..6237)
                     /gene="nreB"
                     /locus_tag="NBH32_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501138.1"
                     /note="Involved in the regulation of the the nitrate
                     reductase operon narGHJI; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation sensor histidine
                     kinase NreB"
                     /protein_id="HCD1514807.1"
                     /translation="MINEDSIQLEALLKKYYEHSIEKIVFADDSGKIIAMNDAAKDIL
                     SEEDNYSAVANAICHRCEGYTNAYDVQSCKDCFLESMQVQATNFQVFMKTKDQKVMPF
                     TATYQLIDQDRGIHAFTLQNVSSQIEQQEKLHQQRMMRKTISAQENERKRISRELHDS
                     VIQEMLNVDVQLRLLKYQEDTAKLLEDAENIEYIVAKLIDDIRNMSVELRPASLDDLG
                     LEAAFKSYFKQFEENYGIKIIYTSNIKNTRFDSDIETVVYRVVQEAILNALKYADVNE
                     INVGIRQTGRHLVAEVIDAGNGFDPSSKPKGSGLGLYGMNERAELVSGSVNIETKIGE
                     GTNVTLNIPI"
     gene            complement(6261..6713)
                     /gene="nreA"
                     /locus_tag="NBH32_001191"
     CDS             complement(6261..6713)
                     /gene="nreA"
                     /locus_tag="NBH32_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate respiration regulation accessory nitrate
                     sensor NreA"
                     /protein_id="HCD1514808.1"
                     /translation="MTPEAIIEDRRFQETLDKIRKEEGYDFAAIAFYESNKPSSPIKW
                     HYVSGNKNNRFKLIILRKGRGLAGTVMKTGKRMIIANVGLALGPEEKIDYPILLSESL
                     TAVLAVPLWYKNQVYGVLLFGQRDGRPLPKIFDNDDIQRKFGIFNDDK"
     gene            complement(6733..7410)
                     /gene="narI"
                     /locus_tag="NBH32_001192"
     CDS             complement(6733..7410)
                     /gene="narI"
                     /locus_tag="NBH32_001192"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCD1514809.1"
                     /translation="MLNQFLWVIFPYLCLVVFVAGHIARYRYDKFSWTAKSSELIERK
                     RLMWGSLLFHLGIIPVFFGHVVGLLIPKSWMDAAGVSEHLYHIGAVYIGSIFGIITLV
                     GMFLLTARRVTTKSIRRLSSASDIFVNFLLLLIVFMGCYATLVTNIQHPDFNYRTSLS
                     IWFRQLFMLKPDASLMSGVPIAFKIHILLGFTIMACWPFTRLVHVWSVPLTYINRRYI
                     IYRKNKV"
     gene            complement(7403..7978)
                     /gene="narJ"
                     /locus_tag="NBH32_001193"
     CDS             complement(7403..7978)
                     /gene="narJ"
                     /locus_tag="NBH32_001193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCD1514810.1"
                     /translation="MINFDNFKKYQESFGYIAQQLCFPEKLTFHPKTFEETISKDHPG
                     YDDLIAFRNVMMTFSLSEIKAIYTDTFDFTKKAPLYMTYNKFDTQKERGQMLAKLKVL
                     YEMFGLEMVDNELSDYLPLMLQFLQVAEWRNDPRAEENIQLIIMIIEDGTYVMANALG
                     EDNNPYAYVIQALRKTLKLCLTSPKGVKHHA"
     gene            complement(7971..9530)
                     /gene="narH"
                     /locus_tag="NBH32_001194"
     CDS             complement(7971..9530)
                     /gene="narH"
                     /locus_tag="NBH32_001194"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCD1514811.1"
                     /translation="MKIKAQVAMVLNLDKCIGCHTCSVTCKNTWTNRPGAEYMWFNNV
                     ETKPGVGYPKRWEDQEHYKGGWVLNRKGKLELKSGSRISKIALGKIFYNPDMPLIKDY
                     YEPWNYNYEHLTTAKSGNHSPVARAYSEITGDNIEIEWGPNWEDDLAGGHVTGPKDPN
                     IQKIEEDIKFQFDETFMMYLPRLCEHCLNPSCVASCPSGAMYKRDEDGIVLVDQDACR
                     GWRYCMTGCPYKKVYFNWKTNKAEKCTFCFPRIEAGMPTVCSETCTGRMRYLGVLLYD
                     ADRVHEAASAVDEKDLYEKQLDIFLNPFDEEVIAQAEKDGISYDWIEAAQNSPIYKLA
                     IEYKLAFPLHPEFRTMPMVWYCPPLSPIMSYFEGKNTNQNPDAIFPAIEEMRLPIEYL
                     ANIFTAGDTKPVKEALQRMAMMRSYMRSQVTQQPFDTSRLERLGLTERQTKDMYRLLG
                     LAKYEDRFVIPTSHKETYLDTYHAQGSTGYNYGGEHFGDNCEGCGVAVGSGKTGQEIY
                     NENFYGGIFRD"
     gene            complement(9520..13209)
                     /locus_tag="NBH32_001195"
     CDS             complement(9520..13209)
                     /locus_tag="NBH32_001195"
                     /EC_number="1.7.99.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCD1514812.1"
                     /translation="MGKFGLNFFKPTEKFNGNWSILESKSREWEKMYRERWSHDKEVR
                     TTHGVNCTGSCSWKVFVKNGVITWENQQTDYPSCGPDMPEYEPRGCPRGASFSWYEYS
                     PLRIKYPYIRGKLWDLWTEALEENNGNRVAAWASIVENEDKAKQYKQARGMGGHVRSN
                     WKDVTDIIAAQLLYTIKKYGPDRIAGFTPIPAMSMISYAAGARFINLLGGEMLSFYDW
                     YADLPPASPQIWGEQTDVPESSDWYNASYIIMWGSNVPLTRTPDAHFMTEVRYKGTKV
                     ISVAPDYAENVKFADNWLAPNPGSDAAIAQAMTHVILQEHYVNQPNERFINYAKQYTD
                     MPFIIMLDEDENGYKAGRFLRASDLGQTTEQSEWKPVIHDTISDSLVVPNGTMGQRWE
                     EGKKWNLKLETEDGSKINPTLSMTEGGYELETIQFPYFDSDGDGIFNRPIPTRQVTLA
                     NGDKVRIATIFDLMASQYGVRRFDHKLESKGYDDAESKYTPAWQEGISGVKQSVVIQV
                     AKEFAQNAIDTEGRSMIIMGAGINHWFNSDTIYRSILNLVMLCGCQGVNGGGWAHYVG
                     QEKCRPIEGWSTVAFAKDWQGPPRLQNGTSWFYFATDQWKYEESNVDRLKSPLAKTEE
                     LKHQHPADYNVLAARLGWLPSYPQFNKNSLLFAEEAKDEGIDSNEAILQRAIDEMKSK
                     QTQFAIEDPDLKKNHPKSLFIWRSNLISSSAKGQEYFMKHLLGTKSGLLATPNEDEKP
                     EEITWREETTGKLDLVVSLDFRMTATPLYSDIVLPAATWYEKHDLSSTDMHPYVHPFN
                     PAIDPLWESRSDWDIYKTLAKAFSEMAKDYLPGTFKDVVTTPLSHDTKQEISTPYGVV
                     KDWSKGEIEAVPGRTMPNFAIVERDYTKIYDKYVTLGPVLEKGKVGAHGVSFGVSEQY
                     EELKSMLGTWSDTNEDSVRTNRPRIDTARNVADAILNISSATNGKLSQKSYEDLEQQT
                     GMPLKDISSERAAEKISFLNITSQPREVIPTAVFPGSNKQGRRYSPFTTNVERLVPFR
                     TLTGRQSYYVDHEVFQQFGESLPVYKPTLPPMVFGDRDKKIKGGTDALVLRYLTPHGK
                     WNIHSMYQDNKHMLTLFRGGPTVWISNEDAEKHDIQDNDWLEVYNRNGVVTARAVISH
                     RMPKGTMFMYHAQDKHIQTPGSEITDTRGGSHNAPTRIHLKPTQLVGGYAQISYHFNY
                     YGPIGNQRDLYVAVRKMKEVNWLED"
     gene            complement(13513..14490)
                     /gene="cobA"
                     /locus_tag="NBH32_001196"
     CDS             complement(13513..14490)
                     /gene="cobA"
                     /locus_tag="NBH32_001196"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCD1514813.1"
                     /translation="MSVDEYGKVYLIGAGPGNPNYLTKKAERLIREAEVILYDRLVNP
                     LILQYANLTTEIIDVGKKPYAKHIQQEKINDCIVEAARRYNKVVRLKGGDPAIFGRVQ
                     EEVDTLNNFNIAFEIVPGVTSASAAVATLQTGLTMRTVAKSVTFSTGHFKDSEENEVD
                     VNSLVNGGTLAIYMGVKRLGKIITQIQQYTDIDYPIAIVFQASCFNEFVVKGHLSNII
                     SKLQHYSIEAKPGICIIGEVVGYTENTPKSYDPTKQFYVVSGAKHDALILCEHLYDEG
                     YGCLLNPNDSSNGTYHSSQYDYYDAFIKQQENVTYISTDRADAKTVLCH"
     gene            complement(14481..14795)
                     /gene="nirD"
                     /locus_tag="NBH32_001197"
     CDS             complement(14481..14795)
                     /gene="nirD"
                     /locus_tag="NBH32_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCD1514814.1"
                     /translation="METKEKIKVTTLDELTPLIGKKVIVKGKEIGLFLTESGTIHAIH
                     NICPHKQGPLSEGTVSGEYVFCPLHDQKIDLNTGIVQEPDEGCVDVYEVEVTDGNVYI
                     CL"
     gene            complement(14799..17204)
                     /locus_tag="NBH32_001198"
     CDS             complement(14799..17204)
                     /locus_tag="NBH32_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1514815.1"
                     /translation="MAKQKLVMIGNGMAGIRTIEEILERANDLYDITVIGKEPYPNYN
                     RIMLSNILQNKMTVEETIMNPYEWYEEHGIELITNDPVIEVDRANQNVTTANGIEVAY
                     DKLIFATGSKAFVIPVPGSTLPSVIGWRTIDDTEKMMDIAKTKKKAIVIGGGLLGLEC
                     ARGLLDQGMEVTVLHLAEWLMEMQLDRKAGNMLKADLEKQGMKFEMQANTTEILGEDD
                     VEGVKLADGREIPADLVVMAVGIRPYTEVAKESGLDVNRGIVVNDVMQTSDSNVYAVG
                     ECAEHNGKVYGLVAPLYEQGKVLADHLTNKETDGYKGSTTFTSLKVSGCELYSAGQIV
                     ENAEIKGIEIFNSVDNNYKKVFLKDGNVVGAVLYGDIDDGSRFYNMMKKGESTEDYTL
                     VSLLTKGGEEASLSIADMADDETICGCNGVDKGTIVNAITENGFTTVEEVTAKTKAGN
                     SCGKCKPQIAQILQHTLGDDFVAAKPAGICGCTDLTRDQIVTQIRAKGLKTSKEVRHV
                     LNFKNKGGCPKCRPAINYYLNMVYPHDHEDERESRFANERYHANIQNDGTFSVIPQMR
                     GGVTDADQLIRLGEVAKKYHVPLVKVTGSQRVGLYGVKKEELPNIWEDLGMRSASAYG
                     KKTRSVKSCVGKEFCRFGTQYTTRLGIRLEKTFEYIDTPHKFKMGVSGCPRSCVESGV
                     KDFGIISVENGFQIYIGGNGGTEVEKAEFLTTVETEDEVIKLCGALMQYYRETGIYAE
                     RTAPWLRRLGFENVKEVLLDPERQNELFERIMDAKKAVEAEPWEAITSNAQARKIFEV
                     EKV"
     gene            complement(17269..18000)
                     /locus_tag="NBH32_001199"
     CDS             complement(17269..18000)
                     /locus_tag="NBH32_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sirohydrochlorin chelatase"
                     /protein_id="HCD1514816.1"
                     /translation="MNGNIIVAHGMRHGRQNQALEAFISELVKDDIHHYDIAFLESEH
                     QDLETVMTTLIKSGVKKLKIVPLLLFSAMHYLKDIPNIVHEMTQRYPDITVEVSEPLG
                     THPLMRRLIEQRICDALAGDEQQVGVMVVAHGNINGKFTKAHEELQTCVQHLQINKPT
                     YARTLYGEISFIHDLEDIARQYQKLIVVPLFLYDGRLVNKVKQQMNDMVINTDIHITP
                     SINFDPILKQIIKDRLESLMIPMKI"
     gene            complement(18233..18769)
                     /locus_tag="NBH32_001200"
     CDS             complement(18233..18769)
                     /locus_tag="NBH32_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1514817.1"
                     /translation="MTKTMIRLASEQDAEKLQQLMHEAFTPLRELGIDWPSVNANLDA
                     VKENIDKNTTFVMTIDDEIISTITVRYPWGSVKSISGYPFVWWFATNPEYEGKGYGSQ
                     LLTYVEETFLRDTLKSAAVTLGTSARLHPWLLKIYEKRGYEIYSEHENDDGDLGVIMR
                     KVLIPERFEETILGQPPF"
     gene            complement(19003..19827)
                     /locus_tag="NBH32_001201"
     CDS             complement(19003..19827)
                     /locus_tag="NBH32_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1514818.1"
                     /translation="MIKNNKTVEDTYSTKAIVDSISSSVQMKQVMVQQTPGRYMLKSM
                     MAGFLLSIVTVFMFGIKTQFASTHVDGLINLMGAIAFSLGLVLVVLTNSELLTSNFMY
                     FTVGWYYKVVSLNKMMWILLYCFLGNILGGFVLFFLMKYAHVMTPEMTQALTALVQKK
                     TVDSTWLNIFTKGIFCNFFINIGIFISMQFKGGLTKAFFIACGVIVFVYMGYEHVVFN
                     AGLYAGMVFFNLDAVSWLHVLKNIAFAFLGNFVGGGIFVGLVYAFLNGKRNRLEQQ"
     gene            complement(20030..20212)
                     /locus_tag="NBH32_001202"
     CDS             complement(20030..20212)
                     /locus_tag="NBH32_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514819.1"
                     /translation="MVQRYIKVLILYIFTTLLSSITVTSKCVPNKVVRFILRTVVGYG
                     IFAYGLHYFSNLKKNK"
     gene            complement(20299..20766)
                     /locus_tag="NBH32_001203"
     CDS             complement(20299..20766)
                     /locus_tag="NBH32_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HCD1514820.1"
                     /translation="MTIKVEDNKIIFSRTIEAPIEKVFDAYTTKALFEKWFHPKDAST
                     KVFRFNAVSGGDAFYAIKTPTMTSYTLAEYKTVKRPYLIEYIDSFATPQGAKDTKMPS
                     MKITLSFSKSNTTKTTVTSTSVFPTKEAAQQVVNMGVEQGMNQTLDQLDALLK"
     gene            complement(20952..22502)
                     /gene="adcA"
                     /locus_tag="NBH32_001204"
     CDS             complement(20952..22502)
                     /gene="adcA"
                     /locus_tag="NBH32_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding
                     lipoprotein AdcA"
                     /protein_id="HCD1514821.1"
                     /translation="MKKKLGMLLLVPAVTLSLAACGNDDGKDKDGKVTIKTTVYPLQS
                     FAEQIGGKHVKVSSIYPAGTDLHSYEPTQKDILSASKSDLFMYTGDNLDPVAKKVAST
                     IKDKDKKLSLEDKLDKAKLLTDQHEHGEEHEHEGHDHEKEEHHHHHGGYDPHVWLDPK
                     INQTFAKEIKDELVKKDLKHKDDYEKNYKKLNDDLKKIDNDMKQVTKDKQGNAVFISH
                     ESIGYLADRYGFVQKGIQNMNAEDPSQKELTKIVKEIRDSNAKYILYEDNVANKVTET
                     IRKETDAKPLKFYNMESLNKEQQKKDNITYQSLMKSNIENIGKALDSGVKVKDDKAES
                     KHDKAISDGYFKDEQVKDRELSDYAGEWQSVYPYLKDGTLDEVMEHKAENDPKKSAKD
                     LKAYYDKGYKTDITNIDIKGNEITFTKDGKKHTGKYEYNGKKTLKYPKGNRGVRFMFK
                     LVDGNDKDLPKFIQFSDHNIAPKKAEHFHIFMGNDNDALLKEMDNWPTYYPSKLNKDQ
                     IKEEMLAH"
BASE COUNT         7066 a         4454 c         3244 g         7877 t
ORIGIN      
        1 ttatgattta acctatactg gttacattgt tttaatggcg attgaaaatg atgaaaaact
       61 taacatcaaa aaattaggtg aacgtgtatt cttagattct ggaacactga caccattact
      121 aaagaaatta gaaaagaaag attacgttgt tcgaacacgt gaagagaaag atgaaagaaa
      181 cctacaaatt tctctaacag agcaaggtaa agcaataaaa agtcctcttg ctgaaatttc
      241 tgttaaagta tttaatgaat tcaacatttc agaacgagaa gcatcagaca ttgttaataa
      301 tttgcggaat tttgtttcga aaaatttcga caattccgac aaaaagtaac tccctatcat
      361 aaggtgatgg gcagaagtat gcatcgctat gtttattcat tatctttatt gcttgtataa
      421 atctcgatgc tcagctcttt tgccctgatt ccttatcagg cacaaatcta cccatttata
      481 actttttatc tcccctttta ctttatcttt tttaatacta ctcatataat ggctttgtaa
      541 tacaaattta tataatggaa gtgatgatat tgaacttcaa tcaattcgag aatcaaaact
      601 tttttaacgg taatccaagt gatacattta aagatttagg taaacaagta tttaattact
      661 tttcaacacc ttcatttgta acgaatatat atgaaacaga cgaattatat tacttagaag
      721 ctgaactagc aggtgtaaat aaagaagata ttagtatcga tttcaataat aatacgctca
      781 ctattcaagc tactagaaac gcaaaacaca aatctgaaca actcatttta gatgagcgta
      841 acttcgaatc attaatgcgt caatttgatt ttgaagctgt tgataagcaa catattactg
      901 ctagttttga aaatgggcta ttaactatta ccctgcctaa aatcaaacca agcaatgaca
      961 ctacttcatc aacatctatt ccaatttcat agttaaattt aaaatgaata cactactaaa
     1021 gccttaatat catgctacga atattgcgca tgatattaag gctttgtcat ttaaatttaa
     1081 gaatgcttcg tattacatat caactgtgtt tcaatgaacc atattctttt tgatataaat
     1141 ggcgcattgt aaataatgca atgcagccga ataccgctaa gaaaataaat gctaaatggc
     1201 ttgaacctgt taaattagct acgtatgtga tgaccaacgg tgggaagaat cctcctaaac
     1261 cacccatcat tgatacgata ccatttgctg atcctgcttc atttgagaag tatgatggta
     1321 ctaatttaaa aattaagccg ttaccaatac ctgcacaaat acttattgtt aaacagccta
     1381 cagtgaataa tgcgatatgg tctgaaatac ctaaaataac ggcaccgata atcataataa
     1441 caaaatcaat catcagtact ttaactgcat taaatttatc acctagtata ccacctattg
     1501 gtctcaagaa tgttgccagc gcaatgaata cacctgaacg aataccagca tctactttgt
     1561 caattccaaa atgatttact aagtagttag gtaagaaaat accaaatgca acaaaagcac
     1621 caaaagtaat gaaataccaa taacttaagt aatataattt gtagttttta gataatgttt
     1681 tcatttgagc cattaatggc actttaattt tacgttcttg tgtgtcacca aaaatgaaca
     1741 taattaatgc aaataaagcg ataataatta agtaacttct aactgttgtt tgccaaccaa
     1801 taatacccgc tatcggtggt gctaaaaatg aagaaactgc tgtaccgata ttccccatac
     1861 cataaatacc atttgctaga cctacttttt ctttagggaa atatttagga actgatgtaa
     1921 caccaacaga gaaaattgcg ccacctacgc caaggaaaaa tcctgatgcc attaacatcc
     1981 ccggcgtttg tgcttggctc aagaaaaata tcgggaataa caatacgata aaacttgtaa
     2041 agaataccca tttagcacca acaatatttg ttaaataacc aaatggcaca cggagcactg
     2101 atcccaaaat aactggtatc gctaaaatga ttgatatttg accttcagtt acattgacat
     2161 cctgtttaat aaaaggcatt aaaggagcaa taatactcca agccataaat ccaaccacta
     2221 aacttaatgt ttgtaatgta agttgaaagc caccttttgt tttgtacata cgtttcacct
     2281 tcaccaaaat catttatttt ttaatagata tcaatactct gcctatatgt tagaggtcaa
     2341 tgttatttaa aaatgctttc atttatatga aaacattcaa tatagacaaa gtattgacta
     2401 catatatatt ttattatgat tgtgaaaaag tgaacataag gattttccct tatttatggt
     2461 gtgatttccc ctatacaatg tccctttacg atgcatttag acactatttt gtcatcattt
     2521 taatcatatt attaaggaac gctcacatac ttttcttaat ttttttcaat tagttacaat
     2581 tactgcttga aaatcacgtt acgttaccct ttatgctgac agcgtaagga ggtttatcat
     2641 gtataaaatc aatattatta gatcagattc cgtctataag aaaatactca aggcaccatt
     2701 aaacgaacgt gattcaattt tcactaaaga aatattagta ccttttaaaa agaaatttga
     2761 ggtgcagcag attcctattc ataatgatga caagcagaca atgagtgcga ttcaactttt
     2821 agatgtattt cataagtctc ccgaagaact ggttgaaact gaccaaatgt caatacaata
     2881 cctgaataat gatttctgga ttaattgtga aaaatattta aaagttgcta tagatcaatt
     2941 tgcaaattat agtatctcat ctcaagtttc taactatcat ttcacagtat tacttggaga
     3001 tagccaaaaa ccacttatgt atctaaataa aaatcgtggt ggtgatggtg gcataccagg
     3061 ttatattatg atttatttag tgccgagtac aagtacaatt aattctatga aaagcttaat
     3121 tgcacatgaa gtaaatcata atatgcgcta tcaatatatt gattgggatg gcggaagttt
     3181 gattgaattg attattgcag aaggattagc tgaaaactat gtagaatcat tgtatggtaa
     3241 agcgcatatt ggaccgtggg taacaaatac taattggagt cgtgataacg taaaaattaa
     3301 aaatactatt tataatcatt tacatttaaa gcatatattt gaatcgatgc cttatctcta
     3361 tggtgatgat attaataaac ttcaaggtag gcctatcgtt ggcttatctc atgctgccgg
     3421 ttatgcatgt ggctatcact tggtgaaata ctttttacaa aaatcaaaca tacctattga
     3481 agttgccaca acacttccag cacaaaaaat aataaatgag gtgactgaat tttggcatac
     3541 acatacactt taaaagatat tattgaaatt acaggtgtaa ctaaaagaac tttacattat
     3601 tacgatgaaa taggattatt agttccagat aaaaatgata aaaattatcg cgtttataaa
     3661 cagcaagact tagaaaaatt acaaaagatt ttaatactca agtcttttga ttttgatatc
     3721 gctaaaataa aacaatacat ttcatatgat aatgaacaat tgcgaaagtt attatcagag
     3781 caaataagca agttagataa aaagatttct gacttacaat taattagacg ctctgtatgt
     3841 gaatttatta aaggacattc tctaatagat accagcattt taaacaagac actacagtca
     3901 caatatgata aagaagcatc tataaaatat ggtcatacga aagcatatca atcatttatt
     3961 agacgtaaag ataacttaca atcacaggat atcagacaga aattgtcaac aatcttcaat
     4021 aaatttaatc atatgtcttt gagtcattat ccaatccaag actgtaatga tatcgtgaca
     4081 gaatggaaat cttttatgaa tactatcgct gattttgatg atgaaacatt atgctgtatt
     4141 gctaaaacat atgaagatga tacgcgtttc aaagattact ttaattcata tgataatcaa
     4201 aatttagcat catacatttc agaagctgtt aattattttt tgagcaatgt gaataagagc
     4261 gacaattttt aatcctcata cagatgctac aacactgaat acaccaacta aaataattgt
     4321 tcaaagtaga aaactaatca tttattctct aaatcaataa ctatattaaa agttgtacct
     4381 ttgcaaagcg aattagtata ggttatcgaa aggagaaagg attaggttcc attcgattta
     4441 taaaaaaaaa tatatttcga aacatacaaa agcgccagaa gattccccgt ttcctctggc
     4501 gctttgtatg gtattatttt caaacattaa aactctaata atttcttttt caatgcatat
     4561 tcaacgagct ctggtttact tttcaagcca agctttgtca taatatgcgt cttatgtgcc
     4621 tctactgtct ttacagaaac aaataatttc tctgcaattt ctttattccc gtaacctttg
     4681 gcaataagtg gtaatatttc tagttctcgt tttgataata ttttaaaagg atctgatgtg
     4741 tttgcagtgt cctgatttga attagataca aattcattca ctaaagatgt tgtaagtttc
     4801 atatctacat atgtttcacc tttatataca gttcgaatag ctaacaataa ttgttcatca
     4861 ggtgcatttt tcaatatgta acctttcgca ccattacgca acacatggaa caaatactcc
     4921 tcatcatcaa acattgttaa tattagtatt ttagtttcag gaaaactgtc agcaatttta
     4981 ctcgtagcga taagacctga ctcacctggt ggcatactta aatccattag taacacatca
     5041 ggtttatatt ccattacttt ttggtaagct tcgacgccat ctgcagccgt tgcaacaact
     5101 tccatatcat tttgataatt taaaatcata gagaaccccg tacggacaac agcgtgatca
     5161 tcggcaatga ctattttcaa ttttattccc ccaatgtatg tttcaaattg gaatgttcaa
     5221 tgtaacattg gtaccctcac caattttcgt ttcgatgttg acgctaccac tgactaattc
     5281 agctcgctca ttcattccat ataaaccgag tccagaacct ttaggcttag aacttggatc
     5341 aaaaccattt cccgcatcta tcacttcagc aaccaaatgt cgcccagttt gacggatacc
     5401 tacatttatt tcatttacat cagcgtattt caacgcattt agaatagctt cttgcactac
     5461 tcgataaaca accgtttcaa tatcactatc aaagcgggta tttttaatat ttgatgtata
     5521 tatgattttt attccataat tttcttcaaa ctgtttaaaa tatgatttaa aagctgcctc
     5581 aaggcctaga tcatccaaag aagcgggtct taattcaacc gacatattac gtatatcatc
     5641 aattaattta gcgacaatat attcaatatt ttctgcgtct tccaaaagct ttgctgtatc
     5701 ttcttgatat ttcaacaatc gcaattgaac atctacattt aacatttctt gaatcacact
     5761 atcatgtaat tccctagaaa ttcgttttcg ctcattttct tgtgcggaaa tcgttttacg
     5821 catcatacgt tgttgatgca atttttcttg ttgttcaatc tgtgatgaga cattttgaag
     5881 cgtaaatgca tgaatccctc tatcctgatc aataagctga taagttgcag taaacggcat
     5941 cactttttga tctttcgtct tcataaacac ttgaaaattc gtagcttgta cttgcatgga
     6001 ctctaagaaa caatctttac atgattgtac atcataggca tttgtatacc cttcacatct
     6061 gtgacaaatc gcattcgcca cagcactgta attatcttct tcagataata tatctttagc
     6121 agcatcattc attgcaataa ttttaccact atcgtcggca aatactattt tttcgattga
     6181 atgttcatag tattttttta ataaagcttc taattgtata ctgtcctcat taatcatgac
     6241 ctacacccta attcatctca ttatttatca tcattgaaaa taccaaactt acgttgaata
     6301 tcatcgttat caaatatttt tggtaaagga cgtccatctc tttgaccaaa taatagtacg
     6361 ccatacactt gattcttata ccaaagcggc actgctaaaa ctgctgttaa tgattcgctt
     6421 aataaaattg gatagtcaat cttttcttct ggtcccaatg ctaaaccgac attagctata
     6481 atcatacgct tccctgtttt cataacagtt ccagctaatc cacggccttt tcttaaaata
     6541 attaatttaa accgattatt tttattacct gaaacatagt gccattttat tggagatgat
     6601 ggtttgttag attcatagaa agcgattgcc gcaaaatcat aaccttcttc tttgcgtatt
     6661 ttatctaatg tctcttgaaa tctacgatct tcaataattg cttctggtgt caaatccttt
     6721 cacctcttat acttacactt tattcttacg gtaaataata tatctgcgat ttatatatgt
     6781 caaaggtaca ctccaaacat gtactaaacg tgtgaatggc caacaagcca taatagtgaa
     6841 acctaataat atatgaattt taaatgcaat tggtacacca ctcattaatg atgcatctgg
     6901 tttcaacata aataattgtc taaaccaaat tgataatgaa gttctatagt taaaatctgg
     6961 atgctgtata tttgttacta atgttgcata acatcccata aatacaataa gtaataataa
     7021 gaagttaaca aagatatcgg acgctgaact taatctgcga atacttttcg tagtaacacg
     7081 tctcgctgtt aataaaaaca tccctaccaa tgtaattata ccaaagatac tccctatata
     7141 aacagcacca atatgatata aatgctcaga cacaccggca gcatccatcc acgacttagg
     7201 tattaataat cctacaacat gtccaaaaaa taccggaata atacctaagt gaaataataa
     7261 actaccccac atcaatcttt ttctttcaat taattcacta gattttgctg tccacgaaaa
     7321 tttatcataa cgataacgtg caatatgacc tgcgacaaag acaactaaac ataaatacgg
     7381 aaatataacc cataaaaact gattaagcat gatgtttcac tccttttggt gatgtcaaac
     7441 ataatttcaa tgtttttcta agcgcttgaa tcacataggc atatggattg ttatcttcac
     7501 caagtgcatt cgccatcaca tatgttccat cctcaataat cataatgatt aattgaatat
     7561 tctcttcagc tcttggatca tttctccatt ctgccacttg taaaaattgc aacatcaacg
     7621 gtagataatc agaaagttca ttgtctacca tttctagtcc aaacatctcg tacaatacct
     7681 ttagtttcgc taacatttgc ccacgttctt tttgtgtatc aaacttgtta tacgtcatat
     7741 ataatggtgc ttttttcgta aaatcaaatg tatctgtata aatcgctttg atttctgata
     7801 aagaaaatgt catcattaca tttctaaatg ctattaaatc gtcatagcct ggatgatctt
     7861 tcgaaattgt ttcttcaaaa gtctttggat gaaatgttaa tttttccgga aagcatagct
     7921 gttgcgctat atatccaaaa ctttcttgat actttttaaa attatcgaaa ttaatcacgg
     7981 aaaatccctc catagaaatt ctcattataa atttcttgac cagtttttcc tgaacctact
     8041 gcaacaccac aaccttcaca gttatcacca aagtgttcgc caccatagtt atatcccgta
     8101 ctaccttgag catgatacgt atctaaatat gtttccttat gtgacgttgg aataacgaat
     8161 cgatcttcat atttagctag tcctaataag cgatacatat ctttagtttg tcgttcagtt
     8221 aaacctaatc gctctaaacg tgatgtatca aatggttgtt gtgtaacttg ggaacgcatg
     8281 taacttctca tcattgccat acgctgcaat gcttctttta ctggttttgt atctcctgcc
     8341 gtgaaaatat tcgctaaata ttcaatcggc aagcgcattt cttcaatggc tgggaaaatc
     8401 gcatccggat tttgatttgt atttttacct tcgaaatagc tcataattgg gctaagtggt
     8461 gggcaatacc aaaccatcgg catcgttcta aattcaggat gtaatggaaa tgctagttta
     8521 tattcaatcg ctaacttata aattggtgag ttttgtgcag cttcaatcca atcataacta
     8581 atgccatctt tttcagcttg tgcaatgact tcttcgtcaa atggatttaa gaatatatct
     8641 aattgttttt cgtataaatc tttctcgtct actgctgaag ctgcttcatg aactcgatct
     8701 gcatcatata ataaaacacc taaataacgc atacgtcctg tacaagtttc agaacacact
     8761 gtaggcatcc ccgcttcaat tctcgggaaa cagaaagtac atttttctgc tttgttcgtt
     8821 ttccagttga agtatacttt tttatatgga caacctgtca tacagtaacg ccaaccacga
     8881 caagcatctt ggtcaactag cacgatgcca tcttcatcac gtttatacat agcacctgaa
     8941 ggacacgatg caacgcaact tggattcaag caatgttcac acaaacgtgg taagtacatc
     9001 ataaatgttt cgtcaaattg gaatttaata tcttcttcta ttttttggat gttaggatct
     9061 tttggacctg taacatgacc acctgctaag tcatcttccc agttaggtcc ccattcaatt
     9121 tcaatgttat cccccgtaat ttctgaatac gctctagcaa ctggagaatg attacctgat
     9181 ttcgcagttg ttaaatgttc ataattatag ttccatggct cgtaataatc tttaattagt
     9241 ggcatatctg gattatagaa tattttccct aatgcaattt ttgaaattct acttccagat
     9301 tttaattcaa gtttcccttt acgatttagt acccaaccac ctttgtagtg ttcttggtct
     9361 tcccaacgtt tcggataccc tacacctggc ttagtctcta cgttgttgaa ccacatgtac
     9421 tcagcacctg gacgatttgt ccaagtgttt ttacatgtca cactacacgt atggcatcct
     9481 atgcatttat ctaaatttaa taccatcgca acttgcgctt taatcttcaa gccaattaac
     9541 ctccttcatc tttctaactg ctacatataa atccctttgg ttcccaattg gtccataata
     9601 attaaagtga taactaattt gtgcatatcc tccgactagt tgtgttggtt tcaaatggat
     9661 tctagtcggc gcgttgtgtg aaccaccacg tgtatctgta atttctgacc caggcgtttg
     9721 aatatgttta tcttgtgcat gatacataaa cattgtacct ttaggcatac gatgcgaaat
     9781 aactgctctt gccgttacaa caccattacg gttatacact tctagccaat cattatcttg
     9841 gatatcgtgt ttttcagcat cttcatttga tatccaaacc gttggaccac ctctaaatag
     9901 tgtcaacata tgcttattat cttgatacat tgagtgtata ttccattttc catgaggcgt
     9961 taaataacgc agtaccaaag catctgtacc acctttaatt ttcttatctc tatcaccaaa
    10021 taccattggt ggtaacgttg gtttatatac tggtaagctt tcaccaaact gttggaaaac
    10081 ttcgtgatcc acataataac tttgacgtcc tgttaatgtt ctaaaaggca ctaaacgttc
    10141 tacattcgtt gtaaatggtg agtaacgtcg gccttgctta ttagatcccg ggaatactgc
    10201 tgttggtata acttcacgtg gttgtgatgt tatatttaaa aacgaaattt tttcagcagc
    10261 acgctcacta gaaatatctt tcaatggcat accagtttgt tgttcaagat cctcatatga
    10321 tttctgtgat aactttccat tcgtagctga tgaaatatta agtatggcat cagcgacatt
    10381 acgtgctgta tcaatacgtg gacgattcgt tctgacagaa tcttcatttg tgtcactcca
    10441 cgtgcctaac atacttttta gttcttcgta ctgctcactg acaccgaaac ttacaccatg
    10501 tgctccaact ttcccttttt caagtacagg accaagcgtg acatatttgt cgtaaatttt
    10561 agtgtagtcg cgttctacaa ttgcaaagtt aggcattgta cgtccaggta ccgcttcaat
    10621 ttcacccttc gaccaatctt tcactacgcc gtatggtgtt gaaatttctt gctttgtatc
    10681 atgactaagt ggagttgtca caacatcttt aaacgttcca ggtaaatagt cttttgccat
    10741 ttctgaaaat gcttttgcca atgttttata aatatcccag tctgaacgtg attcccataa
    10801 tggatcaatg gcaggattga aaggatgtac atatggatgc atatccgttg atgataaatc
    10861 atgtttttca taccaagttg ctgccggtaa aacaatgtca gaatataacg gtgttgccgt
    10921 cattctgaag tctaaagaga ccactaaatc taacttacct gttgtttctt cacgccacgt
    10981 aatttcttct ggcttttcat cttcatttgg tgtagctaat aaccctgatt ttgtgccaag
    11041 taaatgcttc ataaagtatt cttgaccttt tgcagaactt gaaattaagt ttgaacgcca
    11101 tataaataat gattttggat gattcttttt caaatcagga tcttctattg caaattgtgt
    11161 ttgttttgat ttcatttcat caattgcacg ttgcaaaatc gcttcatttg aatctatacc
    11221 ttcatcttta gcttcttctg caaacaacaa gctattttta ttaaattgtg gatatgatgg
    11281 taaccaacca agtctagctg ctaacacatt ataatcagct ggatgttgat gttttaactc
    11341 ctctgtttta gctaatggag attttaaacg atctacattt gactcttcat atttccattg
    11401 gtctgtagca aaataaaacc aacttgttcc gttttgcaaa cgtggtggtc cttgccagtc
    11461 tttcgcaaat gcgacagtac tccatccttc aattgggcga catttttctt gtcccacata
    11521 gtgagcccaa ccgccaccat tcacaccttg acagccacat aacataacta agtttaagat
    11581 tgaacgataa atcgtatctg agttaaacca atggttaata cccgcaccca tgataatcat
    11641 tgaacgccct tcagtatcga tagcgttttg cgcaaattct ttcgctactt gaatgacaac
    11701 actttgtttt acgcctgaaa tgccttcttg ccaagcaggt gtatattttg attctgcatc
    11761 gtcgtatcct tttgattcta atttatgatc aaaacgacgc acgccatatt gacttgccat
    11821 taagtcaaaa attgtagcaa tacggacttt gtcaccattt gctaaagtga cttgtcgagt
    11881 tggaattgga cgattgaata tcccatctcc atcactatca aagtatggga attgaattgt
    11941 ttctaattca tatccacctt ctgtcattga taatgtaggg ttaattttag aaccatcttc
    12001 tgtttctagt tttaagttcc acttcttacc ttcttcccaa cgttgcccca ttgtgccatt
    12061 aggtaccact aaactgtcac tgattgtgtc gtggattacc ggtttccatt cactttgctc
    12121 tgtcgtttgt cctaagtcac ttgctcttaa aaatctaccc gctttatatc cattttcatc
    12181 ttcatctaac atgatgataa atggcatgtc tgtatattgt ttcgcataat taataaagcg
    12241 ttcattcggt tgattaacat aatgctcttg taaaataaca tgtgtcatcg cttgagcaat
    12301 tgcagcatct gaaccaggat tcggggctaa ccagttatca gcaaacttca cattttctgc
    12361 ataatcaggt gctactgaaa taacttttgt tcctttatag cgaacttctg tcataaaatg
    12421 tgcgtctggc gtacgcgtta gtggtacatt agagccccac ataataatgt atgatgcgtt
    12481 ataccagtca cttgattcag gcacatctgt ttgctctccc caaatttgtg gagaggcagg
    12541 tggtaaatct gcataccagt cataaaaact aagcatttca ccaccaagca aattgatgaa
    12601 tcgagcacct gctgcataac taatcatcga catcgctgga ataggtgtaa atcctgcgat
    12661 tcgatctgga ccatattttt taattgtata tagtaattgt gctgcgataa tatccgtaac
    12721 atctttccaa tttgaacgca catgtccacc cataccacgg gcttgcttat actgtttggc
    12781 tttgtcttca ttttcaacaa tagaagccca tgcagccaca cgattgccat tgttttcttc
    12841 taatgcttca gtccataaat cccaaagctt cccacgaata tatggatatt taattcgaag
    12901 tgggctgtat tcataccaag agaatgatgc accacgagga cagcctctcg gttcatattc
    12961 aggcatatcc ggaccacaac taggatagtc agtttgttga ttttcccagg taatcacacc
    13021 atttttcaca aagactttcc atgaacatga tccagtacag ttaacaccat gcgttgttct
    13081 tacttcttta tcatggctcc aacgttctct gtacattttt tcccattctc tacttttact
    13141 ttctaggatc gaccaattcc cattaaattt ttctgttggc ttaaagaaat tcaatccaaa
    13201 ttttcccata tttatatcct cctacgtata aaaatacgat gtgtagatgt cgtgttttta
    13261 aatactaaaa atgcccaaga ctattgcttt aattagattg tacatttttt cacaaatata
    13321 aaatattagg gaattaccta attacttaag gattttccct atcaataatt ggattttatt
    13381 gaaattttac acaatcacat atgaccatgt ttattgccaa tctaaatcgt tcaaatttgg
    13441 cacaacgaca aataaggctt caacacgaat atattctctc ggttgaaacc ttacttattc
    13501 attttttata aattagtgac ataacactgt tttagcatct gcacgatcgg ttgaaatata
    13561 tgttacattt tcttgctgct taataaacgc atcatagtaa tcatattgcg acgaatgata
    13621 tgtgccattc gatgaatcat ttgggtttag caaacagcca taaccttcgt catataaatg
    13681 ttcacagagg ataagggcgt catgtttagc accacttact acataaaatt gcttcgtagg
    13741 atcatatgat ttaggagtgt tttcagtata accaacaact tccccaataa tacatatacc
    13801 tggtttcgcc tcaattgaat agtgttgcaa ttttgaaata atattactta aatgcccctt
    13861 aacaacaaac tcgttaaaac acgatgcttg aaagacaatc gctatcggat aatcaatatc
    13921 tgtgtactgt tgtatctgtg tgataatttt ccctaaacgt tttaccccca tataaatcgc
    13981 taacgtacca ccattcacta aggaattgac atccacttca ttttcttctg aatctttaaa
    14041 gtgacctgta gaaaatgtca cacttttagc aactgtacgc attgtcaaac ctgtctgcaa
    14101 agtagcaact gcagcactcg cagatgtcac accaggtaca atttcaaacg caatattaaa
    14161 gttgtttagt gtgtcaactt cttcttgaac tcgtccaaat attgctgggt cgccaccttt
    14221 aagcctcaca accttgttat atcgacgtgc tgcttccacg atacagtcat ttattttttc
    14281 ttgctgaatg tgttttgcat acggcttttt accaacatcg ataatttcag tagtcaaatt
    14341 cgcatattgt aaaatcaacg gattcactaa tcgatcatat agtataactt ccgcttcacg
    14401 tattaaacgc tcagcctttt tagtcaaata attcggatta cctggtcccg cacctatcaa
    14461 gtaaaccttg ccatattcat ctacagacat atatatacgt tcccgtctgt aacttctacc
    14521 tcataaacat ctacacaacc ttcatcaggt tcttgtacaa tcccagtatt taaatcaatc
    14581 ttttgatcat ggagtggaca gaatacatat tcaccactca ctgttccctc tgacaatggc
    14641 ccttgtttat gcggacaaat attgtgaatc gcgtgaattg taccactttc agttaaaaac
    14701 aatcctattt ctttgccttt gacaataacc tttttaccaa ttaagggtgt caattcatct
    14761 aaagttgtca ctttgatttt ctctttcgtt tccatgtatt acaccttctc cacttcaaag
    14821 atttttcgag cttgcgcatt actagtgatt gcttcccaag gttcagcttc gactgctttt
    14881 ttagcatcca taatacgttc aaataattca ttctgtcttt ctggatcaag caacacttct
    14941 ttaacatttt caaaccctag tcttcttaac cacggtgctg ttctttcagc ataaatacct
    15001 gtctcgcgat aatattgcat caaagcacca cataatttga ttacttcatc ttctgtttct
    15061 acagttgtta aaaattcagc tttttcaact tctgtaccac cattaccacc gatatagatt
    15121 tggaatccat tttcaactga gataatacca aaatctttaa cacctgattc aacacaactt
    15181 cttgggcagc ctgatacacc cattttgaat ttatgaggtg tatcgatgta ttcaaatgtt
    15241 ttttctaaac gaatgccaag tcgtgtcgtg tattgcgtac caaatcgaca aaactctttg
    15301 ccgacacagc ttttaactga gcgtgttttc ttaccataag ctgatgctga acgcatacct
    15361 aagtcttccc atatatttgg taattcttct tttttaactc catacaaacc aacacgttgc
    15421 gaacctgtca ctttaactag tggcacatga tatttcttag ccacttctcc tagacgaatc
    15481 agttggtctg catctgtaac acctccacgc atttgaggta taacagaaaa tgtaccatca
    15541 ttttgaatat tcgcatggta acgttcgtta gcaaatcttg attctctttc atcttcatga
    15601 tcatgtggat aaaccatgtt taaataatag ttgattgctg gtcgacattt tggacatcca
    15661 cctttatttt taaagtttaa aacatgtcga acttctttag atgtttttaa accttttgct
    15721 cttatttgcg ttacaatttg atcgcgtgtc aaatcagtac aaccacatat accagcaggt
    15781 tttgcggcaa caaagtcatc tcctaaggtg tgctgcaata tttgagcaat ttgcggttta
    15841 catttaccac atgaattccc cgcttttgtt ttagccgtta cttcttcaac tgttgtaaag
    15901 ccattttccg taatagcatt tactatagta cctttatcaa caccattaca accacaaatt
    15961 gtttcatcat cagccatatc agcaattgat agcgatgcct cttctccacc tttagtaagc
    16021 aatgatacca gtgtgtaatc ttcagtagat tcaccttttt tcatcatgtt ataaaagcgt
    16081 gaaccatcat cgatatcacc atataagact gcaccgacca catttccatc ttttaaaaat
    16141 acttttttat agttattatc aacactatta aatatttcaa tacctttgat ttcagcattt
    16201 tcaacaattt gaccagcact atatagctca caaccagaaa cttttaatga tgtaaatgta
    16261 gtggaacctt tgtatccatc tgtttcttta ttcgttaaat gatctgccaa tactttacct
    16321 tgttcatata aaggtgcaac taaaccataa actttaccgt tatgttctgc acattcaccg
    16381 actgcatata cattgctatc acttgtttgc atcacatcat tgacaacaat accacgatta
    16441 acatctagac ctgattcttt ggctacttct gtgtatggtc gaatacctac tgccataaca
    16501 actaagtctg ccggaatctc gcgtccatca gccaatttaa caccttcaac atcatcttct
    16561 cctaagattt cagttgtgtt ggcttgcatt tcaaacttca taccttgctt ttctagatct
    16621 gctttaagca tatttccagc tttacggtct aactgcattt ccatcaacca ctcagccaaa
    16681 tgcaacactg ttacttccat accttgatct aataaaccac gtgcacattc taaacctaat
    16741 aagcccccac cgataacaat cgctttcttt ttagtcttag caatgtccat cattttttca
    16801 gtgtcatcaa ttgttctcca gccaataaca cttggtaagg tcgaacctgg cactggaatg
    16861 acaaatgctt tagatcctgt cgcaaatatt aatttatcgt atgctacttc aataccattt
    16921 gcagtcgtta cattttgatt tgctctatct acttcaatta caggatcatt tgtaattaac
    16981 tcgataccat gttcctcata ccactcatat ggattcataa ttgtttcttc aactgtcatt
    17041 ttattttgta aaatatttga aagcatgatg cgattataat taggataagg ttctttacca
    17101 attactgtaa tatcatataa atcattggca cgctctaata tttcttcgat tgttcgaatg
    17161 cccgccatac cgttaccaat cattactagt ttttgctttg ccataaaata tgccccttta
    17221 ctacataata tttatttcaa aaaaaggttt aatttttcgt tagtgctttt atattttcat
    17281 tggaatcatt aagctttcta atctatcttt aatgatttgc tttaaaattg ggtcgaagtt
    17341 aattgaaggt gtgatgtgta tatctgtatt aataaccatg tcattcattt gctgcttcac
    17401 tttgttaaca agtcttccgt catataaaaa taaaggtacg acaatcaatt tttgatactg
    17461 tctcgcaata tcttctaaat catgtataaa actgatttca ccatatagcg ttctcgcata
    17521 agtaggttta ttaatctgca aatgttgaac gcatgtctgt aactcttcat gtgcttttgt
    17581 aaacttgcca ttaatattgc catgtgcaac aaccataact ccaacttgtt gttcgtcacc
    17641 tgctaatgcg tcacaaatac gttgttcaat taatcgtctc attaaaggat gtgtaccaag
    17701 tggctcgctt acttctaccg ttatgtctgg ataacgctgt gtcatttcat gaacgatatt
    17761 cggtatatcc ttgagatagt gcatggcact aaagagaagc aaaggtacaa tcttaagctt
    17821 tttaacgcca cttttaatca acgtcgtcat tactgtttct aaatcctgat gctcactttc
    17881 taaaaatgca atatcatagt gatgtatatc atcttttact aattcagaaa taaatgcttc
    17941 taacgcttga ttctgtcgtc cgtgcctcat gccatgtgca acaatgatat tcccattcac
    18001 atttaccaac cctttcacac gtattgtata ccaaatcatt tcgtttttgt gaaaagaatc
    18061 acattataat gtaaaatcag ggaattccct gatgcctgta gtcatgcata ttccttataa
    18121 attttccctt tttgttaaat caaaaaagcg accaatatgt taatccctac tcaacattca
    18181 ttcatttgag caagcatcaa tatatcggtc gcttgtagtg tatattatta tcttaaaatg
    18241 gtggttggcc tagaatcgtt tcttcaaaac gttcaggtat taatacttta cgcatgatta
    18301 cacctaaatc gccatcatca ttttcatgtt cgctgtatat ttcataacct cttttttcat
    18361 aaattttaag taaccacgga tgcaatcttg cagatgtacc taaagtaact gccgctgact
    18421 ttaacgtatc tcgcaaaaat gtttcttcaa cataagtaag taattggcta ccatagcctt
    18481 tcccttcata ctcaggattt gtcgcaaacc accagacaaa aggatagccc gaaatgcttt
    18541 tcacacttcc ccaaggatat ctgaccgtaa tcgtagatat aatttcatca tcaattgtca
    18601 tgacaaatgt agtattttta tctatatttt ctttcacagc atctaaattt gcatttactg
    18661 aaggccagtc tatgccaagt tctcttagag gcgtaaatgc ttcatgcatg agttgttgca
    18721 atttttctgc atcttgttca cttgcgagtc gaatcatcgt ttttgtcata ttaatcccca
    18781 ctctttttta aatgatttaa ccatatttta tttttaaaat aaatatccat caaagtgtat
    18841 caataaattt atcacatgtc agaaagtatg cttcatctga atacaccaat actctcatga
    18901 aacttattaa aaattactct ctcaacgtaa aaaaaccatt caaattcatg aatggtttgg
    18961 aagaatgatt cattgttacg ctatttaatc actacatctt aattattgtt gctctaaacg
    19021 attacgctta ccatttaaga aagcataaac gagacctaca aaaataccgc caccgacaaa
    19081 gttacctaag aaagcaaaag cgatattttt taaaacgtgt aaccatgaaa ctgcatcaag
    19141 gttgaagaac accatacctg catataaacc agcattgaat acaacgtgct catatcccat
    19201 gtatacaaag actataacgc cacaagcaat gaagaatgct tttgtcaatc cgcctttgaa
    19261 ttgcatagaa ataaaaatac cgatattaat aaagaagtta cagaaaatac cttttgtaaa
    19321 aatattcaac caagtcgaat cgactgtttt tttctgaact aaggcagtta aagcttgtgt
    19381 catttctggt gtcataacat gtgcatattt cattaagaag aataatacaa aaccacctaa
    19441 aatatttcca aggaaacaat ataataaaat ccacatcatc ttattaagag aaacaacttt
    19501 ataataccag ccaactgtaa aatacataaa attacttgtt aataattcag aattcgttag
    19561 tacaactaag accaatccta agctgaaagc aattgctccc attaaattaa tgagaccatc
    19621 aacatgtgta cttgcgaatt gtgttttaat accaaacata aaaacagtaa caatggacaa
    19681 taagaaacct gccatcatag attttagcat gtatcgtcct ggtgtttgct gtaccatcac
    19741 ttgtttcatt tgaacagatg atgatattga gtctacgatt gctttagttg aatacgtatc
    19801 ttcaactgtt ttattatttt ttatcacagc acttccccca tttactatta tcaaataaat
    19861 tttacctatt aattatatta gtatttagat gtaattcata gattaaaatt cagaaaattc
    19921 tttttaccaa ttcaaaacta atatttactc ataaaaatag cgagatagat atcgcgacat
    19981 aaaacaattg gtatcatttt aattgcgctc atctcgcttt atgtaagtat tacttattct
    20041 tttttaaatt tgaaaagtaa tgtaaaccat atgcaaatat accgtagcct acgactgtac
    20101 gcaaaataaa tctaacaact ttatttggta cacatttcga agtaacagta attgaagata
    20161 aaagtgtagt aaaaatatat aatattaata ctttaatgta acgttgtacc atcgaatcat
    20221 ccttcattca attaataata ttacgttatt attataaact gaatggcata taaaaatttt
    20281 tacatatttt caacaacttt atttcaataa agcatcaagt tgatctaatg tttgattcat
    20341 cccttgctca acacccatgt ttacgacttg ttgagcggct tcttttgttg gaaataccga
    20401 tgttgatgta actgtcgttt tcgttgtatt gctcttggaa aatgacaaag taattttcat
    20461 gcttggcatt ttcgtatctt ttgcaccttg aggtgtcgca aatgagtcaa tatattcaat
    20521 taaatatgga cgcttaaccg ttttatattc tgctaatgta tagctggtca ttgtaggtgt
    20581 tttaatcgca taaaatgcat caccgcctga aacagcatta aagcgaaaca ctttggtact
    20641 agcgtctttt ggatgaaacc atttttcaaa taatgctttt gtcgtatagg catcaaacac
    20701 cttttcaatc ggtgcttcta tcgttcttga aaaaataatt ttattatctt caactttaat
    20761 cgtcatcttc ccactcccta tcttttatat ttactcatct taacatgcat tgagtaacat
    20821 atgttaccaa atcatttgtt ataaactata gtaatattaa tcattccctc tgttgaaatt
    20881 gataactacg aaataaaaaa cacactctat tcagctatta acagttgagt agagtgtgca
    20941 aacctgttga attaatgcgc taacatttct tctttgattt ggtctttatt taattttgaa
    21001 ggataatatg ttggccagtt atccatttct tttaataacg cgtcattatc attacccata
    21061 aagatatgga agtgttctgc ctttttaggt gcaatgttgt gatcgctaaa ttggatgaat
    21121 ttcggtaagt ctttatcatt accatcgacc aatttaaaca taaatctcac gccacggtta
    21181 cctttaggat atttcaatgt tttcttacca ttgtattcat atttaccagt gtgtttctta
    21241 ccatctttag taaatgtaat ttcatttcct tttatatcaa tgttagtaat atcagtttta
    21301 tatcctttgt catagtaagc ttttaaatct ttagcagatt tcttcggatc attttcagct
    21361 ttatgttcca tcacttcatc aagcgtaccg tcttttaagt aagggtaaac agattgccat
    21421 tcaccagcat aatcgcttaa ttcacggtct ttaacttgct catctttaaa atacccatca
    21481 gaaattgctt tatcgtgttt actttcagct ttgtcgtctt tcactttaac accactgtct
    21541 aaagctttac cgatattttc aatattcgat ttcattaatg attgatacgt aatattatct
    21601 tttttctgtt gttctttatt taaagactcc atgttgtaga attttaaagg cttcgcatct
    21661 gtttctttac gaattgtttc agtcacttta ttcgcaacat tatcttcgta aagaatatat
    21721 ttagcattgc tatctctaat ttctttaaca attttagtta attctttttg tgatggatct
    21781 tcagcattca tgttttgaat acctttttga acaaaaccat aacgatcagc taagtatcca
    21841 attgattcat gtgaaatgaa tactgcatta ccttgcttat cttttgtaac ttgcttcata
    21901 tcgttatcaa ttttcttaag atcgtcgttt aattttttgt agtttttctc atagtcatct
    21961 ttatgtttta gatctttttt cactaattca tctttaattt ctttagcgaa agtttggtta
    22021 attttaggat ctaaccatac gtgtggatca tatccaccat gatgatgatg atgttcttct
    22081 ttctcatgat catgtccctc atgttcatgc tcttcaccat gctcgtgttg atcagttaaa
    22141 agctttgctt tatctaattt atcctctaaa gacagttttt tatctttatc tttgatagta
    22201 gatgcaactt tcttagcaac cggatctaaa ttatcccctg tatacataaa caagtctgac
    22261 ttgcttgcac ttaatatatc tttttgtgtt ggttcatagc tatgtaaatc tgtccctgct
    22321 ggatagattg atgatacctt cacgtgtttt ccaccaattt gctctgcaaa tgattgcaat
    22381 ggataaactg tcgttttaat tgttaccttg ccatctttat cttttccatc atcattccca
    22441 catgcggcta atgataaagt tacggctggt acaagaagta acatacctaa tttctttttc
    22501 atagtcaccc tcctaaatag taatctttac gttttaaaat tatatactca tgctacttaa
    22561 aatacaagca ctgtttttat aaataatata aagtcgaatc atactttagg tatttaccac
    22621 ttttcgtttt atctaataca t
//
LOCUS       DAHHOJ010000037        22610 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.037, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000037 DAHHOJ010000000
VERSION     DAHHOJ010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22610)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22610)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22610
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.037"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            74..742
                     /gene="queC"
                     /locus_tag="NBH32_001205"
     CDS             74..742
                     /gene="queC"
                     /locus_tag="NBH32_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCD1514822.1"
                     /translation="MESVLNNEKAIVVFSGGQDSTTCLFYAKKHFKEVELVTFNYGQR
                     HDTEIEVAKQIAQDQGMKHHVLDMSLLSQLTPNALTQHDMEITNNEDGIPNTFVPARN
                     LLFLSFAGALAYQIGAKHIITGVCETDFSGYPDCRDSFIKSMNVTLSLAMDKDFVIHT
                     PLMWLNKAETWKLSDELEVLDYIRTKTLTCYNGIIGDGCGECPACHLRQRGLNQYLES
                     KGAL"
     gene            744..1163
                     /gene="queD"
                     /locus_tag="NBH32_001206"
     CDS             744..1163
                     /gene="queD"
                     /locus_tag="NBH32_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCD1514823.1"
                     /translation="MLQQIYPSTTHPYQFELNKDFNFSAAHHIPCEEAGICQNVHGHT
                     YFVNLTIVGDKLDDTGFLVNFSHLKKMIHGKFDHQLLNNLPAFKNKIPSTEIVAETIY
                     QIVKENLASLEHQPKCIQVFVRETPTSYVVFRPKEQV"
     gene            1167..1880
                     /gene="queE"
                     /locus_tag="NBH32_001207"
     CDS             1167..1880
                     /gene="queE"
                     /locus_tag="NBH32_001207"
                     /EC_number="4.3.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HCD1514824.1"
                     /translation="MAKIPVLEIFGPTIQGEGRVIGRKTMFVRTAGCDYRCSWCDSAF
                     TWDGSAKGDIKLMTAEEIYDELKRIGGDLFNHVTISGGNPALIKGIQELVNLFQDKGI
                     FSALETQGSKFQPWMTQIDDLTISPKPPSSTMTPDLKKLDEVITQCVPSSLNLKVVVF
                     DDKDYDFAKMIHHRYPNIPFYLQVGNPYLSDSVDNHTEKLLERYEQLVDLVMQSNDMN
                     HVYVLPQLHTLLWSNKKGV"
     gene            1979..2563
                     /locus_tag="NBH32_001208"
     CDS             1979..2563
                     /locus_tag="NBH32_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514825.1"
                     /translation="MIVYILINIAIVILITGFNLYRHQIQHLSLSAMLLSITINAFIN
                     TFIIDKYNFITLCTITMFIIWTILQFYIDKKLKPVYITDQKFIAIILTIVVSLTQRVT
                     DFSSTQSIYMSIPFLAPAIFIIGGIMLFISTFNNLDETAENNNKIKKLMIKGLIIINI
                     SFIVMMVLTPYWYLYLIVYLIFLLFLLWQKVYKF"
     gene            2843..3283
                     /locus_tag="NBH32_001209"
     CDS             2843..3283
                     /locus_tag="NBH32_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DM13 domain-containing protein"
                     /protein_id="HCD1514826.1"
                     /translation="MNTKYFLAVGAVAAVLTLGACGNSNSQDQGNKTEQKTKSEDSNV
                     KTDKTKHLTGTFSSKNGETVEGKAEIKNGKLMLTNYKSSKGPDLYVYLTKNGDIKNGK
                     EIAMVDYDKEKQTFDLKNVDLSKYDEVTIYCKKAHVIFGGAKLK"
     gene            3626..4099
                     /locus_tag="NBH32_001210"
     CDS             3626..4099
                     /locus_tag="NBH32_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1514827.1"
                     /translation="MNKLLLLVTFIIRVGSGIVMLMQGYEKLTGGFTLKGLVPVIANN
                     TDSPEWYKWFFANIVAHTTSLFDIIVPLGEIAIGLGLIFGVFAYAASFFGAFVMINYI
                     LADMIFTYPLQLTFFILLLMSHSLLKQISLKEIINYFRGRKNRGEKIDDPLTDRG"
     gene            4074..4760
                     /locus_tag="NBH32_001211"
     CDS             4074..4760
                     /locus_tag="NBH32_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1514828.1"
                     /translation="MTHLLIVDDEQDIVDICQTYFEYEGYKVTTTTSGKEAISLLSND
                     IDIMVLDIMMPEVNGYDIVKEMKRQKLDIPFIYLTAKTQEHDTIYALTLGADDYVKKP
                     FSPRELVLRINNLLTRMKKYHHQPVEQLSFDELTLINLSKVVTVNGHEVPMRIKEFEL
                     LWYLASRENEVISKSELLEKVWGYDYYEDANTVNVHIHRIREKLEKESFTTYTITTVW
                     GLGYKFERSR"
     gene            4760..5815
                     /gene="saeS"
                     /locus_tag="NBH32_001212"
     CDS             4760..5815
                     /gene="saeS"
                     /locus_tag="NBH32_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     SaeS"
                     /protein_id="HCD1514829.1"
                     /translation="MVLSIRSQIIIGVVSSILLTSTILAIAYILMWFNGHMTLTLTLT
                     TIITSCLTLLICSIFINPLIQKIKQFNIKTKQFANGNYASNDKTFNSPKEIYELNQSF
                     NKMASEITQQMNQIKSEQQEKTELIQNLAHDLKTPLASIISYSEGLRDGIITKDHEIK
                     ESYDILIKQANRLSTLFDDMTHIITLNTGKTYPPELIQLDQLLVSILQPYEQRIKHEN
                     RTLEVNFCSEIDAFYQYRTPLERILTNLLDNALKFSNVGSRIDINISENKDQDTIDIA
                     ISDEGIGIIPELQERIFERTFRVENSRNTKTGGSGLGLYIANELAQQNNAKISVSSDI
                     DVGTTITVTLHKLDITS"
     gene            complement(5887..6870)
                     /gene="csbB"
                     /locus_tag="NBH32_001213"
     CDS             complement(5887..6870)
                     /gene="csbB"
                     /locus_tag="NBH32_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoteichoic acid-specific glycosylation protein
                     CsbB"
                     /protein_id="HCD1514830.1"
                     /translation="MKIRVVIPCFNEGEVITQTHQQLTEILSQDSSVKGYDYNMLFID
                     DGSTDTTIDEMQHLATIDRHVSYISFSRNFGKEAAMIAGYQHSTEFDAVIMIDCDLQH
                     PPEYIPKMVEGFMEGYDQVIAKRDRSGENFSRKTLSHLYYKLVNCFVEEVQFDDGVGD
                     FRLLSQRAVKSIASLEEYNRFSKGLFEWIGYNTKVFTYQNVERQKGESKWSFKKLFNY
                     GIDGLISFNSKPLRMMIYLGLFIFSISVLYIIYLFINIMISGVNIPGYFSTIAAILLL
                     GGIQLISIGVVGEYIGRIYYEVKARPKYIIQATNLSSIENAEKDTHKVYSK"
     gene            complement(7002..7841)
                     /locus_tag="NBH32_001214"
     CDS             complement(7002..7841)
                     /locus_tag="NBH32_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1514831.1"
                     /translation="MLNEIQILNNGYPMPSVGLGVYKISDEDMTKVVNAAIDAGYRAF
                     DTAYFYDNEASLGRALKDNGVDREDLFITTKLWNDYQGYEKTFEYFNKSIENLQTNYL
                     DLFLIHWPCEADGLFLETYKAMEELYEQGKVKAIGVCNFNVHHLEKLMAQSSIKPMVN
                     QIEVHPYFNQQELQEFCDRHDIKVTAWMPLMRNRGLLDDPVIVKIAEKYHKTPAQVVL
                     RWHLAHNRIIIPKSQTPKRIQENIDILDFNLELTEVAEIDALNRNARQGKDPDDVKIG
                     DLK"
     gene            complement(8063..9412)
                     /locus_tag="NBH32_001215"
     CDS             complement(8063..9412)
                     /locus_tag="NBH32_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1514832.1"
                     /translation="METSTIISLIIFILLIALTTVFVGSEFALVKIRATRIEQLADEG
                     NKPAKIVKKMIANLDYYLSACQLGITVTSLGLGWLGEPTFEKLLHPIFEAINLPTALT
                     TTISFAVSFIIVTYLHVVLGELAPKSIAIQHTEKLALVYARPLFYFGNIMKPLIWLMN
                     GSARVIIRMFGVNPDAQTDAMSEEEIKIIINNSYNGGEINQTELAYMQNIFSFDERHA
                     KDIMVPRTQMITLNEPFNVDELLETIKEHQFTRYPITDDGDKDHIKGFINVKEFLTEY
                     ASGKTIKIANYIHELPMISETTRISDALIRMQREHVHMSLIIDEYGGTAGILTMEDIL
                     EEIVGEIRDEFDDDEVNDIVKIDNKTFQVNGRVLLDDLTEEFGIEFDDSEDIDTIGGW
                     LQSRNTNLQKDDYVDTTYDRWVVSEIDNHQIIWVMLNYEFNEARPTIGQSDEDEKSE"
     gene            complement(9633..10814)
                     /gene="nagA"
                     /locus_tag="NBH32_001216"
     CDS             complement(9633..10814)
                     /gene="nagA"
                     /locus_tag="NBH32_001216"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCD1514833.1"
                     /translation="MSELIIYNGKVYTEDGKIDNGYIHVKDGQIVAIGEVNDKAAIDN
                     DTTNKIQVIDAKGHHVLPGFIDIHIHGGYGQDAMDGSYDGLKYLSENLLSEGTTSYLA
                     TTMTQSTDKIDNALINIAKYEAEQDVHNAAEIVGIHLEGPFISENKVGAQHPQYVVRP
                     FIDKIKHFQETANGLIKIMTFAPEVEGAKEALETYKDDIIFSIGHTVATYEEAVEAVE
                     RGAKHVTHLYNAATPFQHREPGVFGVAWLNDALHTEMIVDGTHSHPASVAIAYRMKGN
                     ERFYLITDAMRAKGMPEGEYDLGGQKVTVQSQQARLANGALAGSILKMNHGLRNLISF
                     TGDTLDHLWRVTSLNQAIALGIDDRKGSIKVNKDADLVILDDDMNVKSTIKQGKVHTF
                     S"
     gene            complement(11122..13080)
                     /locus_tag="NBH32_001217"
     CDS             complement(11122..13080)
                     /locus_tag="NBH32_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCD1514834.1"
                     /translation="MRVTELLTKDTIAMDLMANDKNGVIDELVNQLDKAGKLSDVASF
                     KEAIHNRESQSTTGIGEGIAIPHAKVAAVKSPAIAFGKSKAGVDYQSLDMQPAHLFFM
                     IAASEGGAQTHLDALAKLSGILMDENVREKLLHASSPEEVLAIIDHADDEATKEEEAE
                     AEAQQVAAAEQSSKQSNEPYVLAVTACPTGIAHTYMARDALKKQADKMGIKIKVETNG
                     SSGIKNHLTEQDIENATGIIVAADVHVETDRFDGKNVVEVPVADGIKRPEELINKALD
                     TSRKPFVARDGNRKGHSNDNQEKLSPGKAFYKHLMNGVSNMLPLVISGGILMAIVFLF
                     GANSFDPKSSDYNAFAEQLWNIGSKSAFALIIPILSGFIARSIADKPGFASGLVGGML
                     AISGGSGFIGGIIAGFLAGYLTQGVKAMTRKLPQALEGLKPTLIYPLLTVTATGLLMI
                     YVFNPPASWLNHLLLDGLNNLSGSNIVLLGLVIGAMMAIDMGGPFNKAAYVFATGALI
                     EGNAAPITAAMIGGMIPPLAIATAMLIFRRKFTKEQRGSIIPNYVMGMSFITEGAIPF
                     AAADPLRVIPSMMIGSGIGGAIALGLGSRITAPHGGIIVIVGTDGAHLLQTLIALVVG
                     TLVSALIYGLIKPKLTETEIEASKSMDE"
     gene            complement(13086..14006)
                     /gene="pfkB"
                     /locus_tag="NBH32_001218"
     CDS             complement(13086..14006)
                     /gene="pfkB"
                     /locus_tag="NBH32_001218"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCD1514835.1"
                     /translation="MIYTVTFNPSIDYVIFTNDFKIDGLNRATATYKFAGGKGINVSR
                     VLKTLDVESTALGFAGGFPGKFIIDTLNNSAIQSNFIEVDEDTRINVKLKTGQETEIN
                     APGPHITSTQFEQLLQQIKNTTSEDIVIVAGSVPSSIPSDAYAQIAQITAQTGAKLVV
                     DAEKELAESVLPYHPLFIKPNKDELEVMFNTTVNSDADVIKYGRLLVDKGAQSVIVSL
                     GGDGAIYIDKEISIKAVNPQGKVVNTVGSGDSTVAGMVAGIASGLTIEKAFQQAVACG
                     TATAFDEDLATRDAIEKIKSQVTISVLDGE"
     gene            complement(14003..14767)
                     /locus_tag="NBH32_001219"
     CDS             complement(14003..14767)
                     /locus_tag="NBH32_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCD1514836.1"
                     /translation="MMIITEKRHELILEELSHKDFLTLQELIDRTGCSASTIRRDLSK
                     LQQLGKLQRVHGGAMLKENRMVEANLTEKLATNLDEKKMIAKIAANQINDNECLFIDA
                     GSSTLELIKYIQAKDIIVVTNGLTHVEALLKKGIKTIMLGGQVKENTLATIGSSAMEI
                     LRRYCFDKAFIGMNGLDIELGLTTPDEQEALVKQTAMSLANQSFVLIDHSKFNKVYFA
                     RVPLLESTTVITSEKALNQESLKEYQQKYHFIGGTL"
     gene            complement(15017..15499)
                     /gene="ybaK"
                     /locus_tag="NBH32_001220"
     CDS             complement(15017..15499)
                     /gene="ybaK"
                     /locus_tag="NBH32_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HCD1514837.1"
                     /translation="MAKNKKTNAMRMLDRAKIKYDVHSFEVPEEHLSGVEVAELIQAN
                     VKTVFKTLVLENTKHEHFVFVIPVSETLDMKKAAALVGEKKLQLMPLDNLKNVTGYIR
                     GGCSPVGMKTLFPTVVDKSCENYSHISVSGGLRTMQITIAVEDLIAITKGKIGEVIHE
                     "
     gene            complement(15673..16125)
                     /locus_tag="NBH32_001221"
     CDS             complement(15673..16125)
                     /locus_tag="NBH32_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514838.1"
                     /translation="MTVKNLFLGFVAVILTVCLIGLLILATNEDALAKVHKTINTLNA
                     INVSTEDTYKKKMDILNIHTAKASEVNENVKKQNHFKHRVNANKSNSFNEQECQVIAD
                     RYADKHINDNYGLERISKTNHGYNYVYSNDNSTSKQHVSISNQGIITK"
     gene            complement(16422..17588)
                     /gene="norA"
                     /locus_tag="NBH32_001222"
     CDS             complement(16422..17588)
                     /gene="norA"
                     /locus_tag="NBH32_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorA"
                     /protein_id="HCD1514839.1"
                     /translation="MNKQIFVLYFNIFLIFLGIGLVIPVLPVYLKDLGLTGSDLGLLV
                     AAFALSQMIISPFGGTLADKLGKKLIICIGLILFSVSEFMFAVGHNFSVLMLSRVIGG
                     MSAGMVMPGVTGLIADVSPSHQKAKNFGYMSAIINSGFILGPGIGGFMAEVSHRMPFY
                     FAGALGILAFIMSVVLIHDPKKSTTSGFQKLEPQLLTKIDWKVFITPAILTLVLAFGL
                     SAFETLYSLYTSYKVNYSPKDISIAITGGGIFGALFQIYFFDKFMKYFSELTFIAWSL
                     IYSVIVLVLLVIADGYWTIMVISFVVFIGFDMIRPAITNYFSNIAGDRQGFAGGLNST
                     FTSMGNFIGPLIAGALFDVHIEAPIYMAIGVSLAGVVIVLIEKQHRAKLKQQDL"
     gene            17810..18232
                     /locus_tag="NBH32_001223"
     CDS             17810..18232
                     /locus_tag="NBH32_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1514840.1"
                     /translation="MKLQKSNHTILLVLEKGEDIVECITNFADDQDLTFTSVSGIGAC
                     DDVVLKFFNLTTKQYEEKHITEPLELTSLLGNISRLENGHFAHLHATFGTQSYETFSG
                     HLAKAIVSATAEIILTVTDLDIQRSFKDAVGLNLLDPQ"
     gene            18418..19035
                     /locus_tag="NBH32_001224"
     CDS             18418..19035
                     /locus_tag="NBH32_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1361 domain-containing protein"
                     /protein_id="HCD1514841.1"
                     /translation="MQSRYIARIYFLILFIVSLFETKIFQFMTLNLFLAYIPFELSLL
                     LRLFKPNKKYEWPLFIIYSFIFVLLLPNTFYMVTDLIHLNQFQFNFYAGLNLSEWKYF
                     TYLLLGVFLAVYVMILIYFEILTLTSHRWLNRLIVVVLMFLNGFGIYIGRFLRLHSVY
                     FFNEPLHVLNHILESLTLKTAMFVCFMVVMQAAILLFGKGVRLNK"
     gene            19032..19316
                     /locus_tag="NBH32_001225"
     CDS             19032..19316
                     /locus_tag="NBH32_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514842.1"
                     /translation="MTINIIVLIVSIIVFQLIIGHIWHDIGLSYLRSILLMMLPFGLG
                     VFIQQVSYYERQYPKWQVPQNIKVRLKYIYLATFLEYVVLYLTLFTDILR"
     gene            complement(19471..20844)
                     /locus_tag="NBH32_001226"
     CDS             complement(19471..20844)
                     /locus_tag="NBH32_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCD1514843.1"
                     /translation="MAIAVLLNRMFRMEHNPLFEYIYQQKEDIDACYFIIPEEDMSSA
                     SDLKAQFYRGTLQRFYQSLHAEKLTPYVMSYDDIISFCKENNISEVVIAGDIMSYHLE
                     EYDILHQRSLFNEARIAVTLIRGNHYFKASKTMNQQGEPYKVFTSFYKKWRPYLRHRD
                     VYHYDLKSFEDFVIASPDDLVFDDITFGSSQIIEQNKWQHFLDQDIQNYESGRDYLPE
                     VLTSQLSVALAYGLLDIIEIFNDLLARYDEDEANYEAFIRELIFREFYYVLMTQYPET
                     SYQAFKPKYRQIKWSQNEANFNAWCEGQTGFPIIDAAIMELTQTGFMHNRMRMVVSQF
                     LTKDLFIDWTWGEKFFRKHLIDYDAASNIHGWQWSASTGTDAVPYFRMFNPIRQSERF
                     DAKALYIKTYLPIFNQIDAKYLHDTQRNESNLFEQGIELGSHYPRQMVDHQEKRTQVL
                     ATFKALD"
     gene            complement(20930..22483)
                     /locus_tag="NBH32_001227"
     CDS             complement(20930..22483)
                     /locus_tag="NBH32_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DASS family sodium-coupled anion symporter"
                     /protein_id="HCD1514844.1"
                     /translation="MSEEKHVVEHEQQKKEKTKKQYKPFWIVMSFIILIVVLLLPAPS
                     SLPIMAKAVLAILAFAVIMWVTEAVSYPVSATLIIGLMILLLGFSPVQNLGEKLGNPK
                     SGSAVLAGSDLLGTNHALTLAFSGFATSAVALVAAALFLAAAMQETNLHKRLALLVLS
                     IVGNKTRNIVIGAIIVSIVLAFFVPSATARAGAVVPILLGMIAAFKVSKDSKLASLLI
                     ITSVQAVSIWNIGIKTAAAQNIVAINFINHQLGFDVSWGEWFLYAAPWSIVMSIALYF
                     IMIKVMPPEINTIEGGKDLIKEELHKLGPVSAREWRLIVISMLLLLFWSTEKVLHPID
                     SASITIIALGVMLMPKIGVMTWKHVENKIPWGTIIVFGVGISLGNVLLKTGAAQWLSD
                     QTFGVLGLKHLPIIATIALITLFNILIHLGFASATSLSSALIPVFISLTSTLHLGDQS
                     IGFVLIQQFVISFGFLLPVSAPQNMLAYGTGTFTVKDFLKAGIPLTIVGYILVIVFSM
                     TYWKWLGLL"
BASE COUNT         7565 a         4093 c         3209 g         7743 t
ORIGIN      
        1 ataaattaaa aacgggctta tcaatgccat taacctcttt attaatatta ttaatgatag
       61 gagcaattac aaaatggaaa gtgtattaaa taatgaaaaa gccatcgttg tctttagtgg
      121 tggtcaagac agtacaacat gtctctttta tgcaaaaaaa catttcaaag aagttgaact
      181 cgtaacgttt aattatggcc aaagacatga tactgaaatt gaagtcgcaa aacaaattgc
      241 acaagatcaa ggaatgaaac atcacgtttt agatatgtca ttattatcac aacttactcc
      301 aaacgcatta acacaacatg atatggaaat tactaataat gaagatggta tacctaatac
      361 atttgttcca gctagaaatt tacttttctt gtcgtttgca ggcgctctag cttatcaaat
      421 tggggctaag catattatta caggcgtatg tgaaacagac ttttcaggct acccagactg
      481 tcgcgatagt tttataaaat caatgaacgt aacattaagc ctagctatgg acaaagattt
      541 tgtcattcat actcctttaa tgtggttaaa caaagcagaa acgtggaaat taagtgatga
      601 actcgaagtt ttagattata ttcgtacaaa aacattaaca tgctataacg gtatcattgg
      661 ggatggctgt ggtgaatgtc cagcttgtca tttacgtcaa cgtggactaa atcaatatct
      721 tgaaagtaaa ggagcgcttt aaaatgttac aacaaatcta tcctagtaca acacatccat
      781 atcaattcga attaaataaa gattttaatt tttcagctgc acatcacatt ccatgtgaag
      841 aagcaggtat ttgtcaaaat gtccatggtc atacttactt tgttaattta acaattgtcg
      901 gtgataaact agatgacact ggcttcttag tgaactttag ccatttgaaa aagatgatac
      961 acggtaaatt tgaccatcaa ctgttaaata acttacctgc ttttaaaaac aaaatccctt
     1021 caactgaaat cgtagcggaa acaatttatc aaattgttaa agaaaatttg gcatcgctcg
     1081 aacaccaacc aaaatgtatt caagtatttg taagagaaac accaacaagt tatgtcgtat
     1141 ttagaccaaa ggaacaggtg taactaatgg ctaaaattcc tgtactcgaa atattcggcc
     1201 caaccattca gggtgaaggt cgagtaatcg gtagaaaaac gatgtttgtg agaacagctg
     1261 gatgtgatta tcgctgtagc tggtgtgatt ctgcatttac ttgggatggt agtgctaaag
     1321 gcgatataaa actcatgacc gctgaagaaa tttatgatga attaaaacgt attggtggcg
     1381 atttatttaa ccacgttaca atttctggtg gtaacccagc attaattaaa ggtatccaag
     1441 agttagttaa cttatttcaa gataaaggta ttttcagcgc actggagaca caaggcagta
     1501 aattccaacc ttggatgaca caaattgatg atttaacgat tagtccaaaa ccaccaagtt
     1561 caactatgac accagattta aaaaagttag atgaagtgat tacacaatgt gtaccttcct
     1621 cattaaactt aaaagtagtt gtattcgatg ataaagatta tgattttgcc aaaatgatac
     1681 accaccgtta tccaaatatt cccttctatt tacaagttgg taacccatat ttatcagaca
     1741 gcgtagataa tcataccgaa aagttgttag aacgctacga gcagttggtg gatttagtta
     1801 tgcaaagtaa tgatatgaat cacgtttatg tgttaccaca acttcataca ttactttgga
     1861 gtaataaaaa aggtgtataa aggataactt attttacttt ttacaagtca tatctaatga
     1921 ataacaatat attaattgat ataatagttg attgaagtat aacgaacagg agtccatcat
     1981 gattgtatat atattaatta atattgctat cgtcatactt ataactggat tcaatttata
     2041 taggcatcaa atacaacatt tatcattaag tgcaatgttg ttatctatta cgataaacgc
     2101 ctttataaat acattcataa ttgataaata taactttatt actctgtgta ctataacaat
     2161 gtttatcatt tggacgatac tacagtttta cattgataaa aaattaaaac ctgtatacat
     2221 tacagaccaa aaatttattg ctatcatatt aacgattgtt gtcagtttaa cacaacgtgt
     2281 taccgacttt tcaagtacac aatcaatata tatgtctatt ccatttttag cccctgccat
     2341 tttcatcatt ggtggtatta tgttgtttat ttcaacattt aataacttag atgaaactgc
     2401 tgaaaataat aacaaaataa agaaactaat gattaaagga cttatcatta ttaacatttc
     2461 atttatcgtt atgatggttt taacaccata ttggtacttg tatttaatcg tctatcttat
     2521 tttcttgttg tttttattgt ggcaaaaggt ttataaattt taataccaaa actataaaac
     2581 acttctgata ttcttagttc gatatatcag aagtgtttta tagtgataac tagtttagat
     2641 aaatattttc ttacttaaaa aaccgccctc ttcttatttt gcctcctatt tatttaaatc
     2701 agacaattat tttcattttc aaattattct ttcttcaata ttagttaagc gatatttaaa
     2761 cgaagttaag aattagttaa tggcatatta tttgccttca ttttaaactt aacttatcaa
     2821 attgaagaaa tgaggagtta gcatgaatac aaaatatttt ttagcagttg gtgctgttgc
     2881 cgctgtatta actttaggcg cttgtggtaa ttctaattca caagatcaag gtaacaaaac
     2941 tgaacaaaaa acaaagtcag aagacagcaa tgttaaaact gataaaacaa aacacctaac
     3001 aggtacattc agttctaaaa acggtgaaac tgttgaaggt aaagctgaga ttaaaaatgg
     3061 taaattaatg cttactaact acaaatcatc aaaaggtcca gatttatacg tctacctaac
     3121 aaaaaatggc gacattaaaa acggtaaaga aatcgcaatg gttgactacg ataaagaaaa
     3181 acaaacattt gatcttaaaa atgtagattt aagcaaatat gatgaagtaa caatctattg
     3241 taaaaaagct cacgtcatct tcggcggcgc taaattaaaa taatcaattt ctgagttaaa
     3301 cttttattta caacatacta ttactataca ttacaaattt taaaaatata cattacactc
     3361 attactcaat ggaaagcgta tgatttcaca gcccccccta gcttgtagaa atcatacttt
     3421 ccttttttca atatatatac aactattaaa tcccataaga ttgtagagca cataagtaaa
     3481 tatttttaga gcttgaggtt tgtttagctt aagcagccca tgagctcaaa cacttcctgt
     3541 tcacataaca ctacaaatcg cattatgttg cttaatctta tgtttatata aattacacac
     3601 aataaataga aagaatgtga acatcatgaa taaattattg ctactcgtta catttatcat
     3661 tcgtgtgggt tcaggtattg ttatgttaat gcaaggctac gaaaaattaa cgggcggatt
     3721 tacgctgaaa ggtttagtcc cagtcatagc taacaatact gattcaccag agtggtataa
     3781 gtggtttttc gcaaatatag ttgcacatac gacgtcatta tttgatatta ttgttccact
     3841 tggagagatt gcaattggat taggtttaat ttttggagtt tttgcatatg ctgctagttt
     3901 ctttggagcc tttgttatga taaattatat cttagcagat atgatattta cgtatcctct
     3961 tcaattaact ttctttatcc ttttactaat gagtcactca ttgttaaaac agatttcact
     4021 taaagaaatc attaattact ttagaggtcg taagaacaga ggtgaaaaaa tagatgaccc
     4081 acttactgat cgtggatgat gaacaagaca ttgtagacat ttgtcaaacc tattttgaat
     4141 atgaaggtta caaagtaaca acgacaacta gcggtaaaga agcaatttct ttactatcaa
     4201 atgatattga tatcatggta cttgatatca tgatgccaga agttaatggt tacgacattg
     4261 tcaaagaaat gaaaaggcaa aaattagata tcccctttat ctatttaact gccaaaacac
     4321 aagaacatga taccatttac gccttaactt taggtgcaga tgactatgtc aaaaaaccat
     4381 ttagtccaag ggaactcgtt ttacgtatta ataatttact tacaagaatg aagaaatacc
     4441 atcatcaacc agttgaacaa ctgtcgtttg atgaattaac acttattaac ttaagtaaag
     4501 ttgtgactgt aaatggtcac gaagtcccta tgcgtattaa ggaatttgag ttattgtggt
     4561 atttagcttc tagagaaaat gaagttattt ctaaatcaga attacttgaa aaagtttggg
     4621 gatatgacta ttacgaagat gctaataccg tgaatgtcca tatacaccgt attagagaaa
     4681 aattagaaaa agagagcttt acaacatata ccatcacaac tgtatgggga ttaggatata
     4741 aatttgaaag gagccgataa tggtgttatc aattagaagt caaatcatta ttggcgtcgt
     4801 atcgagtata ctattaactt caactatttt agcaattgca tatattttaa tgtggtttaa
     4861 cggccatatg acactaactt tgaccttaac gacaataatt acaagctgtt taaccttatt
     4921 aatatgtagt atttttatta atccacttat acaaaaaatt aagcagttta atataaaaac
     4981 taagcaattt gctaacggaa attacgcaag caatgataaa acgtttaatt caccaaaaga
     5041 aatttatgaa ttaaatcaat cttttaataa aatggcttct gaaattacgc aacaaatgaa
     5101 tcaaattaaa tccgaacaac aagaaaaaac agaactgatt caaaacttag cccatgattt
     5161 aaaaacacct ttagcaagca ttatttcata ttctgaagga ctacgtgatg gtataatcac
     5221 aaaggatcat gagattaaag agtcatacga catattaatt aaacaagcaa acagattatc
     5281 aacattattt gatgatatga ctcatattat cactttaaat acaggtaaaa catatccccc
     5341 agaattaata caactagacc aattacttgt atcaatattg caaccatatg agcaacgtat
     5401 caaacatgaa aaccgcacat tagaagtgaa tttctgtagc gaaattgatg cattttatca
     5461 atatcgaacg ccacttgagc gtattttaac aaacttactt gataatgcgc taaaattttc
     5521 aaatgttggt agtcgcattg atattaatat tagtgaaaac aaagatcaag atactatcga
     5581 cattgctatt agcgatgaag gtattggcat tataccagaa ctacaagaac gtatattcga
     5641 acgtacattc agagtagaaa actctcgtaa tacaaaaacg ggtggttctg gattaggctt
     5701 atatatagct aatgaactcg cgcaacaaaa taacgcaaaa atcagtgtaa gcagtgatat
     5761 agatgtagga actacgataa ctgtgacatt acacaaatta gacattacgt cataatccga
     5821 tttatttata aaataaaatg caaagactaa aaagaagctc ccattaatga gggcttcttt
     5881 ttttgtttat ttagaataaa ctttatgggt atccttctca gcattttcaa tacttgaaag
     5941 atttgtagct tgaataatat atttaggacg tgccttaact tcataatata tcctgccaat
     6001 atattcacct acaacaccaa ttgaaattaa ctgtatgccg cctaataata aaatagctgc
     6061 aatcgttgaa aaatatcccg gaatattaac accagatatc ataatattga tgaataaata
     6121 gataatatat agaacgctta ttgaaaagat aaataaacca agataaatca tcattctcaa
     6181 aggtttacta ttaaaggaaa tcaatccatc aataccataa ttaaataact ttttaaatga
     6241 ccatttagat tcaccttttt gtctctcaac attttgatac gtaaacactt tagtattata
     6301 gcctatccat tcaaataacc cttttgaaaa tcgattatat tcttcaagtg atgcaatgga
     6361 tttaacagct ctttggctta aaagtctaaa atcaccaaca ccatcatcaa attgtacttc
     6421 ttctacaaag caattaacta acttataata caaatggctt aatgttttgc gactaaaatt
     6481 ttcaccactt ctatcacgct ttgcaatcac ttgatcatag ccttccataa aaccttcaac
     6541 cattttcgga atatattcag gtggatgttg caaatcacaa tctatcatga tgactgcatc
     6601 aaattcagta ctatgctggt aacctgcaat catagctgct tcttttccaa aatttctact
     6661 aaaagaaata tagctgacat gtctatctat tgtggcaaga tgttgcattt catctatagt
     6721 ggtatccgta ctaccatcat ctatgaaaag catattataa tcatagcctt tcacactact
     6781 atcttgtgaa agtatttcag ttaattgttg atgtgtttgt gtaatgactt ccccttcatt
     6841 aaaacaagga atgacgactc taattttcat ctatgtccat cctttttatt taaaagattt
     6901 tgaatgtacc tgagtattgt tttatcaaat agtaaaaacc aactcattta ttttaatgta
     6961 cacattaaaa ggcattcata aacgtaaaat ttaacatcca gttattttaa atccccaatt
     7021 ttcacatcat ctggatcttt accttgtctt gcatttctat ttaaagcatc aatttcagct
     7081 acttctgtta attctaaatt aaaatctaaa atatctatat tttcttgaat gcgtttaggt
     7141 gtctgagatt ttggaataat aattctattg tgtgctaaat gccaacgcaa tacaacttgt
     7201 gctggtgttt tatgatattt ttcagcaatt ttaacaatga cagggtcgtc tagtagtcct
     7261 ctatttctca tcaaaggcat ccatgcagtc actttaatat cgtgacgatc acaaaattct
     7321 tgtaattctt gttggttaaa atatggatgt acctcaattt gattcaccat tggtttgata
     7381 cttgattgag ccattaattt ttctagatga tgaacattaa aattgcatac acctattgct
     7441 tttaccttac cttgctcgta aagttcttcc atagctttat atgtttctaa aaatagacca
     7501 tctgcttcac aaggccaatg tattagaaat aaatcaagat aattagtttg taaattttca
     7561 atcgatttgt tgaaatattc gaatgttttc tcataacctt gatagtcatt ccataacttc
     7621 gttgttataa acaaatcttc tctatcgacg ccattatcct ttaatgctcg acctagtgaa
     7681 gcctcattat cataaaagta tgcagtatca aacgctctat agcctgcgtc aattgcagca
     7741 tttacaactt tagtcatatc ttcgtcagag attttataaa cacctaatcc aactgaaggc
     7801 atcgggtatc cattatttaa tatttgtatc tcattcaaca tctttatatc tctccaatct
     7861 atgtatcttt tatatcttta cattacccta attttttcaa caaactcaat taatacgaat
     7921 tatcgctttc aataaaaaat attcattaaa tcattaaaga tattaagttc caatactatt
     7981 ttcacttttc attaatttta gtcaaaaaaa taaccaacca aaaatgaatt aaatcattct
     8041 tagttggttt atatattatt atctattctg atttttcatc ttcatcagac tgtccgatag
     8101 taggtctcgc ttcattaaac tcatagttta acatcaccca aataatttgg tggttatcga
     8161 tttctgaaac aacccagcga tcataagttg tatccacgta atcatctttt tgtaagttgg
     8221 tattacgaga ttgtaaccat ccacctatcg tatcaatatc ctcagagtca tcaaattcta
     8281 taccgaactc ttcagttaaa tcatccaata gtactctacc atttacttgg aatgtcttat
     8341 tatcaatttt aacgatatca ttcacttcat catcatcaaa ttcatcacga atttccccaa
     8401 cgatttcttc taaaatatct tccatcgtta aaatacctgc cgttccacca tattcatcta
     8461 taataagact catatgtaca tgttcacgtt gcattctaat taatgcatca ctgatacgtg
     8521 ttgtctctga aatcattggc aactcatgta tatagtttgc tattttaata gtttttccag
     8581 aagcatattc agttaaaaat tctttgacgt taataaatcc tttaatgtgg tctttatcac
     8641 catcatcagt aattggataa cgcgtaaatt gatgttcttt tattgtttct agtaattcgt
     8701 ctacattaaa aggttcattt agtgtaatca tttgagttct aggtaccatt atatcttttg
     8761 catgtctttc atcgaatgaa aagatatttt gcatatacgc caattcagtt tggttgattt
     8821 ctccaccatt ataactattg ttaataataa ttttgatttc ttcttctgac attgcatcgg
     8881 tttgggcatc aggatttaca ccaaacattc taataataac acgtgcagaa ccattcatca
     8941 gccaaatcaa tggtttcata atgttaccga aatagaacaa tggtcttgca tatactaaag
     9001 caagcttttc agtatgttga atcgctatag atttaggcgc taattcacca agtactacat
     9061 gcaaatacgt aacgattata aatgatactg caaacgaaat cgtcgtcgtt aatgcagttg
     9121 gtaaattgat tgcttcaaat atcgggtgta atagcttttc aaacgttggt tcaccaagcc
     9181 aacctaaccc taaagatgtt actgttatac ctaactgaca agcagaaaga taataatcta
     9241 gattagcaat catctttttt actattttag caggtttatt tccttcatct gctagctgtt
     9301 caattcttgt tgctctaatt tttactaatg caaattctga accaacaaat acagtggtta
     9361 atgcaattaa tagaataaat ataatcaaac taattatggt cgaagtttcc aattaattcc
     9421 ctatttctag ggattcacct ccatgttata tgtgtcactc atgggtaaca cgcattcaaa
     9481 tttatcacta tgacttaaat tacaactaca tgttatcgcc ttcccattta gacaccccca
     9541 gaaaaaaatg ttattgcttc tattttatca taatataaag tgcttatgtt aacagattaa
     9601 atctattgca tacatttaat tatgattatc tattagctaa atgtgtgaac cttaccttgt
     9661 tttattgtag attttacatt catgtcatca tctagaataa caaggtctgc atccttattt
     9721 actttaatac taccttttct atcatcgata cctaatgcaa tggcttgatt taaacttgtt
     9781 acacgccata aatgatctaa tgtatcacct gtaaatgata ttaagttacg taacccatga
     9841 ttcattttta aaatactacc agcaagcgca ccatttgcaa gacgtgcttg ttgtgattga
     9901 acagttactt tttgtccacc caaatcatat tctccttcag gcataccttt tgcacgcatt
     9961 gcatctgtaa ttaaataaaa acgttcatta cctttcatac ggtaagcaat tgcaaccgat
    10021 gccggatgag aatgtgtgcc atcaacaatc atttcggtat gtagagcatc attcaaccat
    10081 gctactccaa aaacacctgg ttctctatgt tggaatggcg tcgctgcatt atataaatgc
    10141 gtgacatgtt tagctcctcg ctcaacagct tcaactgctt cttcgtatgt tgccactgta
    10201 tgaccaattg aaaaaataat gtcatcttta tacgtttcaa gcgcttcttt tgcaccttca
    10261 acttcaggtg caaacgtcat aatctttatt aatccgttag cagtctcttg aaaatgttta
    10321 attttatcga taaatggacg tacaacgtat tgcggatgtt gagcaccaac tttattttca
    10381 gatataaatg gcccttccaa atgtatacct acaatttccg ctgcattgtg aacatcttgc
    10441 tccgcttcat atttagcaat atttataagt gcattatcta ttttatcagt agattgcgtc
    10501 attgtagtgg ccaagtatga tgtcgtccct tcagacaaca aattttcgga tagatatttt
    10561 aagccatcgt atgacccatc cattgcatct tgaccataac caccatgaat atgtatatca
    10621 ataaaacctg gtaatacatg atgaccttta gcatcaatca cttgaatttt atttgtcgta
    10681 tcattatcaa ttgctgcttt atcatttact tctccaattg caacaatctg tccatctttc
    10741 acatgaatgt aaccattatc gattttgcca tcttcagtat aaactttgcc gttatatata
    10801 attaattctg acacgtataa acccttcttt atttataaat tttcacttta agacactcat
    10861 attttactat atctgcttcg tttggcataa ctttttgcaa aatattaaaa aagtctatac
    10921 atttgtggtg tctatttctt cattcattta tctaaaaata aaaacacctt catatcaatt
    10981 cataaaaact gaattaatag aaagtgtttt tacagcttta tataaactac cgttaacacg
    11041 ataaataaaa ctaacatata ttcattgaac atacaactta atatgaagct ctttgctaac
    11101 aatcatttta aatcattaaa actattcgtc cattgatttt gaagcttcga tttctgtttc
    11161 agttaacttt ggtttaataa gtccataaat tagcgctgaa actaatgtac ctactacaag
    11221 tgcaataagt gtttgcagta aatgtgcacc atccgtaccg acaattacga taataccacc
    11281 gtgtggcgca gtaattcgag aacctaaacc taaagcgatt gcgccaccta tacctgaccc
    11341 aatcatcatt gaagggataa cacgtaatgg atcggcagcc gcgaatggga tcgcaccttc
    11401 tgtaataaag gacataccca tcacatagtt agggataatt gaaccacgtt gttcttttgt
    11461 aaatttacgt ctgaaaatta acatcgctgt cgcaattgct aacggtggaa tcataccacc
    11521 aatcattgca gctgtaattg gtgctgcatt accttcaatc aacgcacctg ttgcaaaaac
    11581 atatgccgct ttgttgaatg gaccacccat atcaatcgcc atcatagcgc caataactaa
    11641 acctaataat acaatattag aacctgataa attgtttaat ccatctaata acaaatgatt
    11701 taaccaagat gctggtggat taaagacata aatcatcaat aatcctgtag ctgtcactgt
    11761 taatagtggg taaattaatg tcggctttaa tccctctaat gcttgtggta acttacgtgt
    11821 catggcttta acaccttgtg ttaagtaacc tgctaagaaa cctgcaataa taccaccaat
    11881 aaatcctgaa ccacctgaaa ttgctaacat accacctaca agacctgacg cgaatccagg
    11941 tttatctgca atactacgtg caatgaaacc agataaaatt gggataatta atgcgaatgc
    12001 acttttacta ccgatattcc aaagttgttc tgcaaacgcg ttataatctg aacttttcgg
    12061 atcaaatgag tttgcaccaa ataaaaacac gattgccatt aaaataccac cagatattac
    12121 aagtggtagc atgttagaaa caccgttcat taagtgttta tagaatgctt tacctgggct
    12181 taatttttct tggttgtcat ttgaatgacc ttttctgtta ccatcacgag caacaaaagg
    12241 tttacgactt gtatctaatg ctttattaat taattcttct ggtcgtttaa taccgtctgc
    12301 gactggtact tctacgacat ttttaccatc aaatcgatcc gtctcaacat gaacatctgc
    12361 agcaacaatg atacctgttg cattttcaat atcttgttca gttaaatggt ttttaatacc
    12421 gcttgaacca ttcgtttcta ctttaatttt aatacccatc ttatccgctt gctttttcaa
    12481 tgcatcacgt gccatatatg tgtgtgcaat acctgttgga caagcagtta cagctaatac
    12541 atatggctca ttagattgtt tagatgattg ttctgcagct gcaacttgtt gtgcttcagc
    12601 ttctgcctct tcttcttttg tcgcttcatc atcagcatga tcaataattg ctaacacttc
    12661 ttctggagac gatgcatgta ataatttctc acgaacattt tcatccatta aaataccaga
    12721 tagtttagct aaagcatcta aatgtgtttg ggcaccacct tctgacgctg caatcataaa
    12781 gaataagtgt gctggttgca tatccaaact ttgataatct acgcctgctt tagatttacc
    12841 aaatgcaata gctggtgact taactgctgc cactttggca tgtggaatag caataccttc
    12901 accgatacca gttgtacttt gtgattctcg attgtgaatc gcttccttaa atgacgcgac
    12961 atcacttaat ttacctgctt tgtctaattg atttactaac tcatcaataa caccattttt
    13021 gtcatttgcc attaaatcca ttgctatcgt atcttttgtt aataactctg ttactctcat
    13081 tattttcact ccccatcaag tacgctaatc gtaacttgtg attttatttt ttctatagcg
    13141 tcccgtgttg ctaagtcctc atcaaatgcc gtggcagtac cgcatgcaac tgcttgttgg
    13201 aatgcttttt caatcgttaa acctgaagca attccagcca ccatgcctgc aactgtacta
    13261 tcaccagagc caactgtatt aaccactttc ccttgtggat taactgcttt aatactgatt
    13321 tctttatcaa tataaatagc accatcaccg ccaagtgaga caataacaga ttgcgcacct
    13381 ttatcaacta acaaacgacc atatttaata acatctgcgt ctgagttcac tgttgtatta
    13441 aacatcactt ctaattcatc tttattaggt ttaataaata gtggatgata tggtaaaacg
    13501 ctttcagcca attctttttc agcgtcgact actaatttag cacctgtctg tgctgtaatt
    13561 tgtgcaattt gcgcatacgc atcgcttgga atactacttg gtacacttcc agcaacaata
    13621 actatatctt cgcttgttgt atttttaatt tgttgtaaca gttgttcaaa ttgtgttgac
    13681 gttatatgag gaccaggtgc attgatttct gtttcttgtc ctgtttttaa tttcacatta
    13741 atacgtgtat cttcatcaac ttcaataaaa ttcgattgaa ttgcactgtt atttaatgta
    13801 tctataatga atttcccagg aaatccacct gcaaatccca aggcagttga ctcaacatcc
    13861 aatgtcttta aaacacgtga gacattaata ccttttcccc cagcgaattt atatgttgct
    13921 gttgctctat tcaaaccatc aattttaaaa tcattcgtaa aaatgacata gtcaattgaa
    13981 ggattgaaag tcactgtata aatcataaag tccctcctat aaagtgatac ttttgttggt
    14041 attcttttaa cgattcttga tttaatgctt tttcagatgt gatgacggtc gtactttcta
    14101 gcaaaggtac acgagcaaaa tatactttat taaacttaga atgatctata agtacaaatg
    14161 attgattggc taatgacatt gctgtttgtt taactaatgc ctcttgctca tcgggagtag
    14221 ttaatccaag ttcaatatct aatccattca tcccgataaa agctttatcg aaacaatatc
    14281 gtcttaatat ctccatggca cttgaaccaa tcgtagcaag tgtattttct ttaacttgac
    14341 cacctagcat aattgtttta ataccttttt taagtaaagc ttctacatgt gttaaaccat
    14401 tggttaccac aatgatatct ttcgcttgaa tatatttaat tagctccaat gtagatgaac
    14461 cagcatcgat aaataagcat tcattatcgt tgatttgatt agctgctatt ttagcaatca
    14521 ttttcttttc atcgagatta gttgctaatt tttcagttaa attcgcctca accatacgat
    14581 tttcttttaa cattgcacca ccatgcacac gttgcaattt ccctaattgt tgtagtttag
    14641 ataaatctct tcgtattgtt gaagcactgc agccagttcg atctattaat tcttgtaaag
    14701 tcaaaaaatc tttgtgcgaa agttcttcta atattaactc gtgtcttttt tctgtaatta
    14761 tcatcatcac cccatctcac tctgtattat aatgagtacg ctttcacttt tcaatcataa
    14821 attaccaaaa acaatcaaaa acaaccaaat tgtgacgata aaattacatt tttatattca
    14881 aatactcatt tgtaatgact gtttatgatc gtaatatgct aattcaatca aaaaggagtg
    14941 tggcagaaat gaattttata aaaatttcat ctctgtcaca ctcctaatct tgccccatac
    15001 tttttgttgt tattaatcat tcatggataa cttctccaat tttgccttta gttattgcaa
    15061 tcaaatcctc aacagctatt gtgatttgca ttgttcgaag cccaccactc acactgatat
    15121 gactataatt ttcacaggat ttgtcaacga ctgttggaaa caatgttttc ataccaacag
    15181 gcgaacaccc accacgaatg tatcccgtta catttttcaa attatctaaa ggcataagct
    15241 gcaatttctt ctctccaacc aaagcagctg cctttttcat atctaaagtt tcacttactg
    15301 ggataacaaa tacaaaatgt tcatgttttg tattttctag aacaagcgtt ttaaatactg
    15361 ttttaacatt tgcttgtatg agttctgcga cttctacacc cgataaatgt tcttctggta
    15421 cctcaaagct atgaacatcg tatttaattt ttgcacgatc aagcatacgc atcgcgttcg
    15481 ttttcttatt tttagccatt tcaacacctc atttatactt cttcagaact atattaacat
    15541 tttaaaatac attacggatt ggttgatttt gacataacat ttacatcatt taataagttg
    15601 agttcacctt ttcaaattaa attattgcaa aataaaaaca gtatatccaa ttagaataca
    15661 ctgttccatc tattatttcg ttattatgcc ttgatttgaa atacttacat gttgcttact
    15721 agttgaatta tcattggaat acacataatt atatccatga tttgtcttag aaattctttc
    15781 taaaccataa ttatcattga tatgcttatc tgcataacga tcagcaataa cttggcactc
    15841 ttgttcgtta aaagaatttg atttatttgc attcacacga tgcttaaaat gattttgctt
    15901 tttcacattt tcattcactt cagatgcttt agcagtatga atattgagaa tatccatttt
    15961 ctttttataa gtatcttcag ttgatacatt tatcgcgtta agtgtattaa tcgttttatg
    16021 taccttagca agcgcatctt catttgttgc taatattaat aaaccaatta aacaaacggt
    16081 taatattaca gcaacaaagc ctaaaaataa attttttact gtcatcccct tacccacatt
    16141 tccttactca ttttttatca tcttaacaca ttaatgttaa tgaattaggt atgtggattg
    16201 caatttaaaa agaatgtaat atttgtaaca tgtttattac tcaaaatcat tttttgttac
    16261 atataacatt taaatataaa gactaaaaca tttaaaagtt tttaatcttt ataaatgtaa
    16321 agcatcgact tttcttcact ataattgtaa gaaaatcgat gcttattttg aaatgcttta
    16381 gttgtcgaaa tacattaatc gaatgacaac catgtgcgat attacaaatc ttgttgtttt
    16441 aacttagctc tatgttgctt ttcaattaaa acaataacaa cacctgctaa tgacacacct
    16501 atagccatat aaattggtgc ttcaatgtgt acatcaaata acgcacctgc gattaaagga
    16561 cctatgaaat tacccatact agtgaatgtc gagtttaacc cacctgcaaa tccctgtcta
    16621 tcaccagcaa tatttgagaa ataatttgta atagcaggtc ttatcatatc gaacccgata
    16681 aagacaacaa agctaattac cataattgtc cagtaaccat cggcaataac taatagcact
    16741 aatacaataa ctgaatatat taatgaccat gcaataaatg ttaactcaga gaagtatttc
    16801 ataaatttat caaagaaata aatttggaaa agtgcaccaa agataccgcc acctgtaatt
    16861 gcaatcgaaa tatctttagg tgaataattt actttatacg atgtatataa agaatacagt
    16921 gtttcaaatg ccgataaacc gaacgctaag acgagcgtta aaattgctgg cgtaataaag
    16981 actttccaat caattttcgt taacaattgg ggctcaagtt tttggaaacc acttgtcgta
    17041 gactttttcg gatcatgaat caatacaact gacattatga aagctaaaat gcctaatgca
    17101 cctgcaaaat aaaatggcat acgatgtgaa acttctgcca taaatccacc aatccctggt
    17161 cctaaaatga atccagaatt gataatcgct gacatgtagc caaagttttt tgctttttga
    17221 tggcttggtg aaacatctgc aatcaaacct gtcacaccag gcattaccat accagcactc
    17281 ataccaccaa tcactctcga taacatcaat accgaaaaat tgtggccaac tgcaaacata
    17341 aattctgaca ctgagaataa aattaaccct atacatataa ttaatttctt ccctaatttg
    17401 tcagctagcg taccaccaaa cggcgatata atcatttgag ataacgcaaa agcagcaact
    17461 agtaatccta aatcactacc agttaatccc aaatctttta aataaacagg caagactggt
    17521 attactaaac cgatacctaa aaaaatcaag aaaatattaa aatataagac aaaaatctgt
    17581 ttattcatat gctcacctct tttttctcca tgtcatgctt aaagctgtta ttcttctttt
    17641 ccacgacaga ttgcaattca tgctaaatat tcgtaaaaag tttctatatt gtattacaac
    17701 attgtattac aacattgcta catttgacaa tattttttct ttgtaattat cactattttc
    17761 catttaattg tataattaat gacatattaa aataaagggg attgatcata tgaaattgca
    17821 aaaaagtaat catactatac tactagtgtt agaaaaaggc gaagatatcg ttgaatgtat
    17881 aacaaacttt gcagatgacc aagacttaac atttacatct gtcagcggca ttggtgcgtg
    17941 tgatgacgtc gttttaaaat ttttcaattt aacaacgaaa caatacgaag aaaaacatat
    18001 tactgagcca ctggaattga cgagcttatt aggtaatatt tctcgtctag aaaatggtca
    18061 ttttgcacat ttgcatgcta ctttcggtac tcaatcatat gaaactttta gcggacatct
    18121 cgctaaagca attgtttctg caactgctga aatcattctt actgtaactg atttggacat
    18181 tcaacgttca ttcaaagatg cagtcggttt aaatttactt gatcctcaat agcactttac
    18241 tttttagttg aatcaaattt tattacaagt tatcataagt gcttaatcat aaatgcattc
    18301 tcgtttatta atatacttca acgagttaaa ttaaattttt aattaggaat tgaatgcgtt
    18361 tataactatc acatttgaat gaataacttt ttcattagaa agtagtgttt attattgatg
    18421 caatctcgat acatcgctag aatttacttt ttaattttat ttatcgtctc cctttttgag
    18481 actaaaattt ttcaatttat gactttaaat ttgtttttag catatatacc ttttgagctt
    18541 agtctattat tacgattatt caaaccgaat aaaaagtatg aatggccact atttatcata
    18601 tatagtttta tatttgtgtt acttttacca aatacatttt atatggtaac tgatttaatc
    18661 catttgaacc aatttcaatt caacttttac gcaggactaa atttaagtga gtggaaatac
    18721 tttacctatt tactgcttgg tgtcttctta gcggtttatg tgatgatttt aatctatttt
    18781 gaaatactaa cattgacttc tcaccgttgg cttaatagat taattgttgt cgtgctaatg
    18841 tttttaaatg gattcggaat ttatattggt cgtttcttac gcttacattc cgtttatttc
    18901 tttaatgagc ccttacatgt tctaaatcac attctagaaa gtctaacgct taaaactgct
    18961 atgtttgtat gttttatggt tgtcatgcaa gcagcaattc tattatttgg aaaaggagtg
    19021 cgtttgaaca agtgacaata aatataatcg ttttaatagt atctatcatt gtctttcaat
    19081 tgattattgg acacatatgg catgatattg gtttaagtta tttaagaagt atcttactca
    19141 tgatgctgcc atttggacta ggtgtattta tacaacaagt gtcgtattac gaacgacaat
    19201 atcctaaatg gcaagtgcct caaaacataa aagttagatt gaaatatatt tatctcgcta
    19261 cgtttttaga gtatgtcgta ctatacctca ctttatttac agacatacta agataacatc
    19321 aaattaataa gaaaactaaa tacaacttat atatttgagg aaagccaatt tctatcacat
    19381 caataataga aattggctta tttatattgc ttaagaactt tatattctgc tccagatttt
    19441 actcatgcca gatctatcaa ttgagccgaa ttagtctagc gctttaaatg tagctaaaac
    19501 ttgtgtacgt ttttcttgat gatctaccat ttgtcttgga taatgactac ctaattcaat
    19561 cccctgttca aaaaggttgg actcattgcg ttgtgtatca tgcaaatatt ttgcatcaat
    19621 ttgattaaaa atcggaagat atgttttgat atacaaagct ttagcatcaa agcgttcact
    19681 ctgtcttatt ggattaaaca ttctaaaata cggcactgca tccgtacctg tagaagcaga
    19741 ccattgccat ccatgaatat ttgatgctgc atcatagtca ataaggtgct ttctaaagaa
    19801 tttttctccc catgtccaat ctataaataa atctttggtt aaaaattgcg acacaaccat
    19861 tctcattcga ttatgcataa aaccagtttg tgtcaattcc attattgctg catcaatgat
    19921 tggaaatcct gtttgccctt cgcaccatgc attaaaattc gcttcatttt gcgaccattt
    19981 tatctgtcga tatttaggtt tgaaagcttg gtatgaggtt tcaggatact gtgtcattaa
    20041 cacataataa aattctctaa aaatgagttc acgtataaat gcttcatagt ttgcctcatc
    20101 ttcatcataa cgcgccaata aatcattaaa aatttcaata atatctaata atccatatgc
    20161 taaagcaaca cttagctgac ttgttaacac ttcaggtaaa tagtctcttc cgctttcgta
    20221 attctgtata tcttgatcta aaaaatgttg ccatttattc tgttcaatta tttgtgagga
    20281 tccaaatgtt atgtcatcaa acactaaatc atcaggtgat gcaatgacga agtcttcgaa
    20341 tgattttaaa tcatagtgat atacgtctct atgcctcaag taaggtcgcc attttttata
    20401 gaaactagta aaaactttgt atggctcccc ttgttgattc attgttttac tcgctttaaa
    20461 gtaatgattc ccacgtatta aagtaacggc aatacgtgct tcattgaata aagaacgttg
    20521 atgtaaaata tcatattctt caagatgata actcataata tcacccgcaa tcactacttc
    20581 agatatattg ttttctttac aaaatgaaat gatatcgtca taagacatga cataaggtgt
    20641 gagcttttct gcgtgcaacg attggtaaaa gcgctgcaaa gtaccgcgat aaaactgtgc
    20701 tttcaaatca gaagctgaag acatgtcctc ttccggaatg ataaaataac atgcatcaat
    20761 gtcttctttt tgttgataaa tatattcaaa taatggattg tgttccattc gaaacattcg
    20821 atttaataac acagcaattg ccataaacaa aacacccttc aattatcttt ttattttatt
    20881 aagctttttg tcactttaaa ataacctaga ttcttaatta tatttttaat taaagcaaac
    20941 ctaaccattt ccaataagtc atactaaaaa ctatcactag aatataccct acaattgtca
    21001 atggtatacc tgccttcaag aaatctttaa ccgtaaaagt accagtgcca taagccaaca
    21061 tattttgagg tgcactaacc ggtaataaga acccaaagct aataacaaat tgttgaatta
    21121 aaacaaatcc tatagactgg tctcctaagt gtaacgtaga ggttagcgaa ataaaaacag
    21181 gtattaaggc tgatgataaa cttgttgcac tcgcaaagcc caaatgaatc aatatattaa
    21241 aaagcgtgat aagtgcaatt gtcgcgataa taggtaaatg ttttaaacct aaaacaccaa
    21301 aagtttgatc acttaaccat tgagctgcac ctgttttcaa aagaacgtta cctaatgaaa
    21361 tacctacacc aaacacgata attgttcccc atggtatttt attttcaaca tgtttccatg
    21421 tcatgacacc aattttcggc attaacataa cacctaaagc aataatagta atggatgcag
    21481 agtcaatcgg atgtaatact ttttcagttg accaaaacag taataataac atggatataa
    21541 caattaaacg ccattcacgt gcgctaacgg ggccaagttt atgcaattct tcttttatta
    21601 aatctttgcc accttctatt gtattgattt ctggaggcat cactttaatc atgatgaaat
    21661 ataaagctat ggacataact atggaccaag gcgctgcata taagaaccac tcgccccatg
    21721 aaacatcaaa tcctaattga tggtttataa aattaatcgc tacgatattt tgtgctgccg
    21781 ccgttttgat accaatattc caaattgaca cagcttgtac tgaggtaatt attaataaag
    21841 acgctaactt gctatctttg gaaactttaa atgccgcaat catacccagc aagattggta
    21901 caactgcccc tgctctagct gttgcagaag gaacgaaaaa tgcaagtaca attgaaacaa
    21961 taattgctcc aataactata tttctagttt tattaccaac aattgataac actaaaagag
    22021 ctagtctttt atgcaaattc gtttcttgca tagcagcagc caaaaataat gcggctgcaa
    22081 cgagagctac agctgaagtt gcaaatccac taaacgctaa tgttaatgca tgattagttc
    22141 ctagaaggtc acttccagct aaaacagcac tgccactttt cggattacct agcttctccc
    22201 ctaaattttg aaccgggcta aatcctaaaa gtaatatcat taagccaata attaaagttg
    22261 ctgacaccgg atatgataca gcttccgtta cccacataat aactgcaaaa gctaaaatag
    22321 ctagtactgc cttagccatt attggcagac ttgaaggtgc cgggagtaat agtacaacta
    22381 taagtattat aaaactcatg acaatccaaa atggcttgta ttgctttttt gtcttttctt
    22441 tcttttgttg ttcatgttcc actacatgtt tttcttctga cataacagtc acccctttgt
    22501 ttaatcttaa tttaattata acaatttttt aaaacataca tttttaattt atgcaaaata
    22561 cacaatttta tcgaaatatt ttgtaaataa tattgctagg tgatgagtta
//
LOCUS       DAHHOJ010000038        22318 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.038, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000038 DAHHOJ010000000
VERSION     DAHHOJ010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 22318)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22318)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22318
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.038"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            371..1282
                     /gene="rapZ"
                     /locus_tag="NBH32_001228"
     CDS             371..1282
                     /gene="rapZ"
                     /locus_tag="NBH32_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCD1514845.1"
                     /translation="MDNNEKEKSKSELLVVTGLSGAGKSLVIQCLEDMGYFCVDNLPP
                     VLLPKFVELMEQGNPSLRKVAIAIDLRGKELFNSLVAVVDKVKSESDVIIDVMFLEAS
                     TEKLISRYKETRRAHPLMEQGKRSLINAINDEREHLSQIRSIANFVIDTTKLSPKELK
                     ERIRRYYEDEEFETFTINVTSFGFKHGIQMDADLVFDVRFLPNPYYVVDLRPLTGLDK
                     DVYNYVMKWKETEIFFEKLTDLLDFMIPGYKKEGKSQLVIAIGCTGGQHRSVALAERL
                     GNYLNEVFEYNVYVHHRDAHIESGEKK"
     gene            1279..2274
                     /locus_tag="NBH32_001229"
     CDS             1279..2274
                     /locus_tag="NBH32_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HCD1514846.1"
                     /translation="MRQIKVVLIGGGTGLSVMARGLREFPIDITAIVTVADNGGSTGK
                     IRDEMDIPAPGDIRNVIAALSDSESVLSQLFQYRFEENQISGHSLGNLLIAGMTNITN
                     DFGHAIKALSKILNIKGRVIPSTNTSVQLNAVMEDGEIVFGETNIPKKHKKIDRVFLE
                     PNDVQPMEEAIDALREADLIVLGPGSLYTSVISNLCVNGISDALIHSDAPKLYVSNVM
                     TQPGETDGYSVKDHIDAIHRQAGQPFIDYVICSTQTFNAQVLKKYEEKHSKPVEVNKA
                     ELEKESINVKTSSNLVEISENHLVRHNTKVLSTMIYDIALELISTIPFVPSDKRK"
     gene            2385..3329
                     /gene="whiA"
                     /locus_tag="NBH32_001230"
     CDS             2385..3329
                     /gene="whiA"
                     /locus_tag="NBH32_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HCD1514847.1"
                     /translation="MSFASEMKNELTRIDVDEMNAKAELSALIRMNGALSLSNQQFVI
                     NVQTENATTARRIYSLIKRVFNVEVEILVRKKMKLKKNNIYICRTKMKAKEILDELGI
                     LKDGIFTHEIDHSMIQDDEMRRSYLRGAFLAGGSVNNPETSSYHLEIFSQNESHAEGL
                     TKLMNSYELNAKHLERKKGSITYLKEAEKISDFLSLIGGYQALLKFEDVRIVRDMRNS
                     VNRLVNCETANLNKTVSAAMKQVESIKLIDKEIGIENLPDRLREIARIRVEHQEISLK
                     ELGEMVSTGPISKSGVNHRLRKLNDLADKIRNGEQIEL"
     gene            3892..4479
                     /gene="clpP"
                     /locus_tag="NBH32_001231"
     CDS             3892..4479
                     /gene="clpP"
                     /locus_tag="NBH32_001231"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCD1514848.1"
                     /translation="MNLIPTVIETTNRGERAYDIYSRLLKDRIIMLGSQIDDNVANSI
                     VSQLLFLQAQDSEKDIYLYINSPGGSVTAGFAIYDTIQHIKPDVQTICIGMAASMGSF
                     LLAAGAKGKRFALPNAEVMIHQPLGGAQGQATEIEIAANHILKTREKLNRILSERTGQ
                     SIEKIQKDTDRDNFLTAEEAKEYGLIDEVMVPETK"
     gene            4661..6295
                     /locus_tag="NBH32_001232"
     CDS             4661..6295
                     /locus_tag="NBH32_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCD1514849.1"
                     /translation="MKGIELLNNPFLNKGTAFTNEERKQLGLEGLLPANVRTLEQQAE
                     QCYEQFKAKQTDFEKRLFLMAIFNRNRTLFYKLTSKHLVEFMPIIYDPVIAQSIEQYN
                     ENFSRPQDAVFLSVDRQDQIEQELRNVANGRDIRLIVVTDAEGILGIGDWGVDGVDIA
                     IGKLIVYTAAAGINPSQVLPVSLDVGTNNEQLLKDDLYLGNRHKRVDDETYYAFVDKF
                     VSAVRKEYPNALLHWEDFGRGHAKNILDKYEDTLPTFNDDIQGTGIVTLAGVLGALNI
                     SKVDYTNQTFLVYGGGTAGMGITNILKDELIKQGVSEEKANQHFFIMDKQGLLFDDMD
                     DLTEAQQVFAKNRNEFSNTVNWKNLKEVIENIKPTVLIGTSTQTGAFTEDAVKEMLKH
                     TERPMIFPLSNPTKLAEATAQDLLKWTNGKALIGTGIPYNDIEYNGVNYSIGQANNAL
                     MYPGLGLGLIASKAKKVNQQILSAASHALGGMVNPDEPGAAVLPPVEKIHQCSRAIAK
                     AVAQSVIDQGLNAETIDDIDKAIEAEIWYPEYKSYI"
     gene            complement(6504..7406)
                     /locus_tag="NBH32_001233"
     CDS             complement(6504..7406)
                     /locus_tag="NBH32_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCD1514850.1"
                     /translation="MKQYLITGGTGMVGSQLVNEIKKSDSHITILTRHDQISNDKKIS
                     YVNWAKSGWEHKVPQNIDVVINLAGATLNKRWTPEYKQTLMLSRIQSTQALYELFKSR
                     NKAPKVLFNASATGYYPPDLFMSYTEVYKTLPFDFLSDIVYQWERFAQQFEQLGTRVV
                     IGRFGIILSNEGGALQTMKLPYEYYIGGKLGSGQQWYSWIHINDLIQAILFLINNESA
                     SGPFNLTAPIPERQNLFGYTLARAMHKPHETWAPSLAMRLILGQMSTVVLDTQKVLPN
                     KIQALGFQFKYSNLKMALEDLISK"
     gene            8109..8738
                     /locus_tag="NBH32_001234"
     CDS             8109..8738
                     /locus_tag="NBH32_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4887 domain-containing protein"
                     /protein_id="HCD1514851.1"
                     /translation="MSNQNYDYNKNEDGSKKKMSTTAKVVSIATVLLLLGGLVFAIFA
                     YVDHSNKAKERMLNEQKQEQKEKRQKENAEKERKKKQQEEKEQNELDSQANQYQQLPQ
                     QNQYQYVPPQQQAPTKQRPAKEENDDKASKDESKDKDDKASQDKSDDNQKKTDDNKQP
                     AQPKPQPQQPTPKPNNNQQNNQSNQQAKPQAPQQNSQSTTNKQNNANDK"
     gene            9437..10450
                     /locus_tag="NBH32_001235"
     CDS             9437..10450
                     /locus_tag="NBH32_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCD1514852.1"
                     /translation="MKDLLQAQQKLIPDLIDKMYKRFSILTTISKNQPVGRRSLSEHM
                     DMTERVLRSETDMLKKQDLIKVKPTGMEITAEGEQLISQLKGYFDIYADDNRLSEGIK
                     NKFQIKEVHVVPGDADNSQSVKTELGRQAGQLLEGILQEDAIVAVTGGSTMACVSEAI
                     HLLPYNVFFVPARGGLGENVVFQANTIAASMAQQAGGYYTTMYVPDNVSETTYNTLLL
                     EPSVINTLDKIKQANVILHGIGDALKMAHRRQSPEKVIEQLQHHQAVGEAFGYYFDTQ
                     GQIVHKVKTIGLQLEDLESKDFIFAVAGGKSKGEAIKAYLTIAPKNTVLITDEAAAKI
                     ILE"
     gene            10503..11513
                     /gene="gap"
                     /locus_tag="NBH32_001236"
     CDS             10503..11513
                     /gene="gap"
                     /locus_tag="NBH32_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCD1514853.1"
                     /translation="MAVKVAINGFGRIGRLAFRRIQEVEGLEVVAVNDLTDDDMLAHL
                     LKYDTMQGRFTGEVEVVDGGFRVNGKEVKSFSEPDASKLPWKDLNIDVVLECTGFYTD
                     KDKAQAHIEAGAKKVLISAPATGDLKTIVFNTNHQELDGSETVVSGASCTTNSLAPVA
                     KVLNDDFGLVEGLMTTIHAYTGDQNTQDAPHRKGDKRRARAAAENIIPNSTGAAKAIG
                     KVIPEIDGKLDGGAQRVPVATGSLTELTVVLEKQDVTVEQVNEAMKNASNESFGYTED
                     EIVSSDVVGMTYGSLFDATQTRVMSVGDRQLVKVAAWYDNEMSYTAQLVRTLAYLAEL
                     SK"
     gene            11652..12842
                     /locus_tag="NBH32_001237"
     CDS             11652..12842
                     /locus_tag="NBH32_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCD1514854.1"
                     /translation="MAKKIVSDLDLKGKTVLVRADFNVPLKDGEITNDNRIVQALPTI
                     QYIIEQGGKIVLFSHLGKVKEESDKAKLTLRPVAEDLSKKLDKEVVFVPETRGEKLEA
                     AIKDLKEGDVLLVENTRYEDLDGKKESKNDPELGKYWASLGDVFVNDAFGTAHREHAS
                     NVGISTHLETAAGFLMDKEIKFIGGVVNDPHKPVVAILGGAKVSDKINVIKNLVNIAD
                     KIIIGGGMAYTFLKAQGKEIGISLLEEDKIDFAKDLLEKHGDKIVLPVDTKVAKEFSN
                     DAKITVVPSDSIPADQEGMDIGPNTVKLFADELEGAHTVVWNGPMGVFEFSNFAQGTI
                     GVCKAIANLKDAITIIGGGDSAAAAISLGFENDFTHISTGGGASLEYLEGKELPGIKA
                     IDNK"
     gene            12964..13725
                     /locus_tag="NBH32_001238"
     CDS             12964..13725
                     /locus_tag="NBH32_001238"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCD1514855.1"
                     /translation="MRTPIIAGNWKMNKTVQEAKDFVNALPTLPDSKEVESVICAPAI
                     QLDALTTAVKEGKAQGLEIGAQNTYFEDNGAFTGETSPVALADLGVKYVVIGHSERRE
                     LFHETDKEINKKAHAIFKHGMTPIICVGETDEERESGKANDVVGEQVKKAVAGLSENQ
                     LKSVVIAYEPIWAIGTGKSSTSEDANEMCAFVRQTIADLSSKEVSEATRIQYGGSVKP
                     NNIKEYMAQTDIDGALVGGASLKVEDFVQLLEGAK"
     gene            13728..15245
                     /locus_tag="NBH32_001239"
     CDS             13728..15245
                     /locus_tag="NBH32_001239"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCD1514856.1"
                     /translation="MAKKPTALIILDGFANRESEHGNAVKLANKPNFDRYYNKYPTTQ
                     IEASGLDVGLPEGQMGNSEVGHMNIGAGRIVYQSLTRINKSIEDGDFFENDVLNNAIA
                     HVNSHDSALHIFGLLSDGGVHSHYKHLFALLELAKKQGVEKVYVHAFLDGRDVDQKSA
                     LKYIEETEAKFNELGIGQFASVSGRYYAMDRDKRWEREEKAYNAIRNFDAPTYATAKE
                     GVEASYNEGLTDEFVVPFIVENQNDGVNDGDAVIFYNFRPDRAAQLSEIFANRAFEGF
                     KVEQVKDLFYATFTKYNDNIDAAIVFEKVDLNNTIGEIAQNNNLTQLRIAETEKYPHV
                     TYFMSGGRNEEFKGERRRLIDSPKVATYDLKPEMSAYEVKDALLEELNKGDLDLIILN
                     FANPDMVGHSGMLEPTIKAIEAVDECLGEVVDKILDMDGYAIITADHGNSDQVLTDDD
                     QPMTTHTTNPVPVIVTKEGVTLRETGRLGDLAPTLLDLLNVEQPEDMTGESLIKH"
     gene            15375..16679
                     /gene="eno"
                     /locus_tag="NBH32_001240"
     CDS             15375..16679
                     /gene="eno"
                     /locus_tag="NBH32_001240"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017466665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCD1514857.1"
                     /translation="MPIITDVYAREVLDSRGNPTVEVEVLTESGAFGRALVPSGASTG
                     EHEAVELRDGDKSRYLGKGVTKAVENVNEIIAPEIIEGEFSVLDQVSIDKMMIALDGT
                     PNKGKLGANAILGVSIAVARAAADLLGQPLYKYLGGFNGKQLPVPMMNIVNGGSHSDA
                     PIAFQEFMILPVGATTFKESLRWGTEIFHNLKSILSKRGLETAVGDEGGFAPKFEGTE
                     DAVETIIQAIEAAGYKPGEEVFLGFDCASSEFYENGVYDYSKFEGEHGAKRTAAEQVD
                     YLEQLVDKYPIITIEDGMDENDWDGWKQLTERIGDRVQLVGDDLFVTNTEILAKGIEN
                     GIGNSILIKVNQIGTLTETFDAIEMAQKAGYTAVVSHRSGETEDTTIADIAVATNAGQ
                     IKTGSLSRTDRIAKYNQLLRIEDELFETAKYDGIKSFYNLDK"
     gene            17030..17488
                     /locus_tag="NBH32_001241"
     CDS             17030..17488
                     /locus_tag="NBH32_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514858.1"
                     /translation="MADRTNKEIKTGRFIATASIVFSILLIIHYFVSLDNATAKALLN
                     LTNQNTSDKAIDYILNSFRFTGIMYILAYLAGFITFWNRHTYVWWFMFAVYVSNSLFT
                     LINLSITIQAIKAAHGAYLTLPILIVIIGSVALAIYMLVVSIKRKSTFNR"
     gene            17555..17788
                     /gene="secG"
                     /locus_tag="NBH32_001242"
     CDS             17555..17788
                     /gene="secG"
                     /locus_tag="NBH32_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCD1514859.1"
                     /translation="MHTFLIVLLIIDCIALITVVLLQEGKSSGLSGAISGGAEQLFGK
                     QKQRGVDLFLNRLTIILSILFFVLMICISYLGM"
     gene            17904..18644
                     /locus_tag="NBH32_001243"
     CDS             17904..18644
                     /locus_tag="NBH32_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase"
                     /protein_id="HCD1514860.1"
                     /translation="MQIKLPKPFFFEEGKRAVLLLHGFTGNSSDVRQLGRFLQKKGYT
                     SYAPQYEGHAAPPEEILQSSPFVWFKDALDGYDYLVEQGYDEIVVAGLSLGGDFALKL
                     SLNRDVKGIVTMCAPMGGKTEGAIYEGFLEYARNFKKYEGKDQETIDNEMHHFKPTET
                     LKELSEALDTIKDQVDEVFDPILVIQAENDNMIDPQSANYIYEHVDSDDKNIKWYSES
                     GHVITIDKEKEQVFEDIYQFLESLDWSE"
     gene            18678..21050
                     /gene="rnr"
                     /locus_tag="NBH32_001244"
     CDS             18678..21050
                     /gene="rnr"
                     /locus_tag="NBH32_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCD1514861.1"
                     /translation="MNLKQSIEEIINQPEYEPMSVSDFQDALGLSSADSFRDLIKVLV
                     ELEQSGLIERTKTDRYQKKHSSKGHSKLIKGTLSQNKKGFAFLRPEDEDMEDIFIPPT
                     KINRALDGDTVIVEIHQSKGEHKGKIEGEVKSIEKHSVTQVVGTYSEARHFGFVIPDD
                     KRIMQDIFIPKGQSLGAVDGHKVLVQITKYADGSDNPEGHISAILGHKNDPGVDILSI
                     IYQHGIEIEFPDEVLQEAEAVPDHIENTEIKGRHDLRDELTITIDGADAKDLDDAISV
                     KKLANGNTQLTVSIADVSYYVTEDSALDKEAYDRATSVYLVDRVIPMIPHRLSNGICS
                     LNPHVDRLTLSCRMEIDASGRVVKHEIFDSVIHSDYRMTYDAVNQIITEKDPAIREQY
                     NEITPMLDLAQDLSNRLIQMRKRRGEIDFDISEAKVLVNEDGIPTDVQLRQRGEGERL
                     IESFMLIANETVAEHFSKLDVPFIYRVHEQPKSDRLRQFFDFITNFGIMIKGTGEDIH
                     PTTLQKVQEEVEGRPEQMVISTMMLRSMQQAHYDDVNLGHFGLSAEYYTHFTSPIRRY
                     PDLTVHRLIRKYLIEKSMDNKEVKRWEDKLPELAEHTSKRERRAIEAERDTDELKKAE
                     YMIQHIGDEFEGIVSSVANFGMFIELPNTIEGMVHIANMTDDYYRFEERQMALIGERQ
                     AKVFRIGDTIKVKVTHVDVDERLIDFQIVGMPLPKNDRSQRPARGKTIQAKTRGKSLD
                     KSKSDDKGHKKKGKQRKGKNERKNDKSGNSKHKPFYKDKSVKKKARRKKK"
     gene            21072..21536
                     /gene="smpB"
                     /locus_tag="NBH32_001245"
     CDS             21072..21536
                     /gene="smpB"
                     /locus_tag="NBH32_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCD1514862.1"
                     /translation="MAKKKSPGTLAENRKARHDYNIEDTIEAGIVLQGTEIKSIRRGS
                     ANLKDSYAQVKNGEMYLNNMHIAPYEEGNRFNHDPLRSRKLLLHKREIIKLGDQTREI
                     GYSIVPLKLYLKHGHCKVLLGVARGKKKYDKRQALKEKAVKRDVARDMKARY"
     gene            21627..21988
                     /gene="ssrA"
                     /locus_tag="NBH32_001246"
     tmRNA           21627..21988
                     /gene="ssrA"
                     /locus_tag="NBH32_001246"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT         7985 a         3247 c         4311 g         6775 t
ORIGIN      
        1 atttctttat cgttgtccca ccccaactcg cattgcctgt agaatttctt ttcgaaattc
       61 tctgtgttgg ggcccacacc ccaacttgca ttgtctgtag aaattggaaa tccaatttct
      121 ctgtgttggg gcccctgact agagttgaaa aaagcttgtt gcaagcgcgt tttcattcag
      181 tcaactacta gcaatataat attatagacc ctaggacatt gatttatgtc ccaatctcct
      241 tttaaatgat gtatattttt agaaatttaa tctaggcata gttaaaaata aatataaaac
      301 atcgttgctt aattttgtca tagaacattt aaattaacat catgaaattc gttttggcgg
      361 tgaaaaaata atggataata atgaaaaaga aaaaagtaaa agtgaactat tagttgtaac
      421 aggtttatct ggcgcaggta aatctttggt tattcaatgt ttagaagaca tgggatattt
      481 ttgtgtagat aatctaccac cagtgttatt gcctaaattt gtagagttga tggaacaagg
      541 aaatccatcc ttaagaaaag tggcaattgc aattgattta agaggtaagg aactatttaa
      601 ttcattagtt gcagtagtgg ataaagttaa aagtgaaagt gacgtcatca ttgatgttat
      661 gtttttagaa gcaagtactg aaaaattaat ttcaagatat aaggaaacgc gtcgtgcaca
      721 tcctttgatg gaacaaggta aaagatcctt aatcaatgca attaatgatg agcgagagca
      781 tttgtctcaa attagaagta tagctaattt tgttatagat actacaaagt tatcacctaa
      841 agaattaaaa gaacgcattc gtcgatacta tgaagatgaa gagtttgaaa cttttacaat
      901 taatgtcaca agtttcggtt ttaaacatgg aattcagatg gatgcagatt tagtatttga
      961 tgtacgattt ttaccaaatc catattatgt agtagattta agacctttaa caggattaga
     1021 taaagacgtt tataattatg ttatgaaatg gaaagagacg gagattttct ttgaaaaatt
     1081 aactgatttg ttagatttta tgatacctgg gtataaaaaa gaagggaaat ctcaattagt
     1141 aattgccatc ggttgtacgg gtggacaaca tcgatctgta gcattagcag aacgactagg
     1201 taattatcta aatgaagtat ttgaatataa tgtttatgtg catcataggg acgcacatat
     1261 tgaaagtggc gagaaaaaat gagacaaata aaagttgtac ttatcggtgg tggcactggc
     1321 ttatcagtta tggctagggg attaagagaa ttcccaattg atattacggc gattgtaaca
     1381 gttgctgata atggtgggag tacagggaaa atcagagatg aaatggatat accagcacca
     1441 ggagacatca gaaatgtgat tgcagcttta agtgattctg agtcagtttt aagccaactt
     1501 tttcagtatc gctttgaaga aaatcaaatt agcggtcact cattaggtaa tttattaatc
     1561 gcaggtatga ctaatattac gaatgatttc ggacatgcca ttaaagcatt aagtaaaatt
     1621 ttaaatatta aaggtagagt cattccatct acaaatacaa gtgtgcaatt aaatgctgtt
     1681 atggaagatg gagaaattgt ttttggagaa acaaatattc ctaaaaaaca taaaaaaatt
     1741 gatcgtgtgt ttttagaacc taacgatgtg caaccaatgg aagaagcaat cgatgcttta
     1801 agggaagcag atttaatcgt tcttggacca gggtcattat atacgagcgt tatttctaac
     1861 ttatgtgtca atggtatttc agatgcgtta attcattctg atgcgcctaa gctatatgtt
     1921 tctaatgtga tgacgcaacc tggggaaaca gatggttata gcgtgaaaga tcatatcgat
     1981 gcgattcata gacaagctgg acaaccgttt attgattatg tcatttgtag tacacaaact
     2041 ttcaatgctc aagttttgaa aaaatatgaa gaaaaacatt ctaaaccagt tgaagttaat
     2101 aaggctgaac ttgaaaaaga aagcataaat gtaaaaacat cttcaaattt agttgaaatt
     2161 tctgaaaatc atttagtaag acataatact aaagtgttat cgacaatgat ttatgatata
     2221 gctttagaat taattagtac tattcctttc gtaccaagtg ataaacgtaa ataatataga
     2281 acgtaatcat attatgatat gataatagag ctgtgtaaaa aaatgaaaat agacagtggt
     2341 tctaaggtga atcatgtttt aaataagaaa ggaatgactg tacgatgagc tttgcatcag
     2401 aaatgaaaaa tgaattaact agaatagacg tcgatgaaat gaatgcaaaa gcagagctca
     2461 gtgcactgat tcgaatgaat ggtgcactta gtctttcaaa tcaacaattt gttataaatg
     2521 ttcaaacgga aaatgcaaca acggcaagac gtatttattc gttgattaaa cgtgtcttta
     2581 atgtggaagt tgaaatatta gtccgtaaaa aaatgaagct taaaaaaaat aatatttata
     2641 tttgtcgtac aaagatgaaa gcgaaagaaa ttcttgatga attaggaatt ttaaaagacg
     2701 gcatttttac gcatgaaatt gatcattcaa tgattcaaga tgatgaaatg agacgcagtt
     2761 acttgagagg agcttttctg gcaggtggct cagtgaataa ccctgaaaca tcttcgtacc
     2821 atttggaaat tttttctcaa aatgagagtc atgcagaagg cttaacgaaa ctaatgaata
     2881 gttatgagtt gaatgccaaa catttagagc gaaaaaaagg aagtattacg tatttaaaag
     2941 aagcggaaaa gatttcggat tttcttagtt tgataggtgg ctatcaagcg ttattaaaat
     3001 ttgaagacgt acgtattgta agagatatgc gtaattctgt taaccgactc gttaattgtg
     3061 aaacggcaaa tctaaataaa acagttagtg ctgcgatgaa acaagttgag agcattaaat
     3121 tgattgataa agaaattggt attgaaaatt taccagacag gttgagagag attgctagaa
     3181 ttcgagtaga acatcaagaa atttcgttga aagagcttgg agaaatggta tcaactggtc
     3241 caatttcaaa atcaggtgta aatcaccgat taagaaaact taatgattta gccgataaga
     3301 ttagaaatgg tgaacaaata gaattataag taagaaggtg tttttggtag tgaattatca
     3361 aaaacacttt tttatgatta aaaagtgttt aatgaataat agtcgactaa ctattaaatt
     3421 gagctggaaa gtttaatgaa ggaaattcaa taaccaagtg atgcctaatt ttctcattaa
     3481 atagcatcat atgtatcaat ttggtcataa gaaaatatga atgtgataat taattgagtc
     3541 cctgatcata gacaaaaaca aaatcagcat aaataagaat cccgacattg cgggattctc
     3601 agtattgaaa agtattgtat tttattaaaa cagcctcctt gaagggaatt gaacccctat
     3661 cttaagaacc ggaatcttac gtgttatcca ttacaccaca aggagcaaat atgtaatcat
     3721 atctctgtac tatgagaagt aaaataagta caatctaaaa tagatattat cagtttacaa
     3781 aggaaagaga aaagcgtcaa acaatgtaac tatttaaagt caaagtgttt gaccaaattt
     3841 gacttaatat gtaaaataat gagtaacagt tattacaagg aggaaatata gatgaattta
     3901 attcctacag ttattgaaac aacaaaccgc ggtgaacgtg catatgatat atactcacgt
     3961 ttattaaaag accgtattat tatgttaggt tcacaaattg atgacaacgt agcaaattca
     4021 atcgtatcac agttattatt cttacaagcg caagactcag agaaagatat ttatttatac
     4081 attaattcac caggtggaag tgtaacagct ggttttgcga tttatgatac aattcaacac
     4141 attaaacctg atgttcaaac aatttgtatc ggtatggctg catcaatggg atcattctta
     4201 ttagcagctg gtgcaaaagg taaacgtttc gcgttaccaa atgcagaagt aatgattcac
     4261 caaccattag gtggtgctca aggacaagca actgaaatcg aaattgctgc aaatcacatt
     4321 ttaaaaacac gtgaaaaatt aaaccgcatt ttatcagagc gtactggtca aagtattgaa
     4381 aaaatacaaa aagacacaga tcgtgataac ttcttaactg cagaagaagc taaagaatat
     4441 ggcttaattg atgaagtgat ggtacctgaa acaaaataat tcaaagtaaa gagtagacta
     4501 agcagtctgc tctttttgta tgagtaaacc aaggtatcaa taaattgttt aaaatatttt
     4561 gagtaaaagc aaaaaataac gggtaaaaat atttgtgaga atttaattag acacgttgtt
     4621 tctgcggttc tacaatctaa caaatataaa ggatgatttt atgaaaggca ttgaattact
     4681 taacaatcca tttttaaaca aaggtacagc ttttactaac gaagaaagaa aacagttagg
     4741 gcttgaaggt ttattacctg cgaatgttcg aactttagaa caacaagcag aacaatgtta
     4801 tgaacaattc aaggcgaaac aaaccgattt tgagaaacgt ttatttttaa tggcaatttt
     4861 taatcgtaat cgtacattgt tttacaaatt gacttcaaag catttagttg aatttatgcc
     4921 aattatttat gaccctgtta ttgctcaatc gattgaacaa tataatgaga acttcagcag
     4981 accccaagat gcagtatttt tatctgttga tagacaagac caaattgaac aagaattgcg
     5041 caacgttgca aatggcagag atattcgatt aattgtagtg acagatgcgg aaggtatatt
     5101 aggtatcggc gattggggtg ttgatggtgt agatattgcg ataggaaaat taatagtata
     5161 tacagcagca gctggaataa atccttcaca agtactacct gtatctttag atgtaggtac
     5221 taataatgaa caattactaa aggatgacct ttatttagga aatcgtcata aacgtgtcga
     5281 tgatgagaca tattacgcat ttgtagataa atttgtgagt gcagtgcgta aagaatatcc
     5341 taatgcacta ttacattggg aagattttgg tagagggcat gccaaaaata ttttagataa
     5401 atatgaagat acgttaccaa cttttaatga tgatattcaa ggtacaggta tagttacatt
     5461 agctggcgta ctaggtgcat taaatatttc taaagtagat tatacgaatc agacgttttt
     5521 agtttatggt ggtggtacag ctggtatggg tattaccaat attttaaaag atgaactaat
     5581 taaacagggt gtttcagaag aaaaagcaaa tcagcatttc ttcattatgg ataaacaggg
     5641 attgttgttt gatgatatgg atgatttaac tgaagcgcaa caagtatttg ctaaaaatag
     5701 gaatgaattt tcaaatacag taaactggaa aaatttaaaa gaagtaattg aaaatattaa
     5761 gcctactgtt ttaatcggta catcaactca gacaggtgca tttacagaag atgcagtaaa
     5821 agagatgtta aaacatacag aacgtccaat gattttccca ttgtctaatc ctacaaaatt
     5881 agcagaagca actgcacaag atttattaaa atggactaac ggaaaagcat taattggaac
     5941 tggtatccca tataatgaca ttgaatacaa tggtgtgaat tacagtattg gtcaagcaaa
     6001 taatgcattg atgtatcctg gtttaggttt gggacttatt gcgtcaaaag cgaagaaagt
     6061 aaatcaacag attctatcag ctgcgagtca tgcacttggt ggcatggtca atccagatga
     6121 accaggtgct gcagtcttac cacctgtcga gaaaatacat caatgttctc gtgccatagc
     6181 caaagcggtt gcacaaagtg tcattgatca aggattaaac gctgaaacta ttgatgatat
     6241 tgacaaagca attgaagctg aaatttggta tcctgaatac aaatcataca tttaatttta
     6301 taaagtagta aaaggtcatt tgaatatttt gtttcaagtg gccttttcgt atttacttta
     6361 aaattaatca accaatattc ataagtgact ttaaagttat atctatatgt aatatcagca
     6421 aatagaaaag tctagagaga tttaacgaat tatctcctag actttttgct tgaagtggag
     6481 taggagagag atcaatgtcc cactcactta ctaattaaat cttcaagtgc catttttaaa
     6541 ttactatatt taaattggaa tcccaatgct tgaattttat taggtaatac tttttgagta
     6601 tccaatacta ctgttgacat ttgaccaagt atgagacgca ttgcaagact tggtgcccaa
     6661 gtttcatgag gcttatgcat agctcttgct aaagtgtagc caaataaatt ttgacgctca
     6721 ggtataggtg cagttaaatt aaacggacca ctagctgact cgttatttat taaaaataaa
     6781 atagcttgaa ttaaatcatt gatatgaatc catgaatacc attgttgacc agaacctaat
     6841 ttaccaccaa tgtaatattc gtatggcagt ttcattgttt gtaacgcacc gccttcattc
     6901 gataaaatta taccgaaacg accgatgaca actcgtgtac ctaattgttc aaattgttgt
     6961 gcgaaacgtt cccattgata cacaatatct gataagaaat caaaaggtaa agttttataa
     7021 acttctgtgt aactcataaa taaatcagga ggatagtaac cagtggcact agcattaaat
     7081 aaaactttag gtgctttatt acgtgattta aacaattcat ataaagcttg cgtagattga
     7141 attctactta gcattagcgt ttgtttatat tccggtgtcc atcgtttatt caatgtagca
     7201 cctgctaagt tgatgaccac atcgatattt tgaggaactt tgtgttccca cccagattta
     7261 gcccagttga catatgaaat tttcttatca tttgaaattt ggtcgtgtcg cgttaatatc
     7321 gtgatatgtg aatctgattt tttaatttca ttaactaatt gagatccaac cataccagtc
     7381 ccaccagtaa ttaagtattg tttcattatc attcacccca tgtaattttg tatttagtta
     7441 ctatttaata atcattattg ttgttcaaag gttatacatt atttagacaa taatatgtca
     7501 ataacttttt ttgaattgta ttttaataaa aatgatataa gttataataa aggtgtacct
     7561 tcgataacga ataaacatct cttaaaagta tgtgtaaaac gctgcatgat acaaacgaag
     7621 gtaaaaattt gactcccttt agtagtggac ccgtacgttt atcgtgcggg tcgtttttat
     7681 ttatttctta ttcccattat acatcaattt aaagcattaa ttttttaaac aaatttaaga
     7741 atacatagta atataacaat ctaaacataa aaacttttaa cccaacgctt aaactaatgc
     7801 tttaattttc aatacgtagc tataatttgt tgtaaaatca aaaaggttaa tatgttaatt
     7861 ttcaaaaaaa ggctcaaaat atgtttgatt tagttattaa atgttaagat atataagact
     7921 actatttctt tgtaaaaatg aatccgattt acgagtgagt aatagtgaag gcagttttaa
     7981 gttgaagaag gcaaaaagag taaatgttta ttaatatttg tagaaactag gtaaacaaat
     8041 tagttgtgaa aatgttaatg gttgcgtgat aatttctata tttaaattag tttgaagtga
     8101 gggagagtat gtcgaatcaa aattacgact acaataaaaa tgaagatgga agtaagaaga
     8161 aaatgagtac aacagcgaaa gtagttagca ttgcgacggt attgctatta ctcggaggat
     8221 tagtatttgc aatttttgca tatgtagatc attcgaataa agctaaagaa cgtatgttga
     8281 acgaacaaaa gcaggaacaa aaagaaaagc gtcaaaaaga aaatgcagaa aaagagagaa
     8341 agaaaaagca acaagaggaa aaagagcaga atgagctaga ttcacaagca aaccaatatc
     8401 agcaattgcc acagcagaat caatatcaat atgtgccacc tcagcaacaa gcacctacaa
     8461 agcaacgtcc tgctaaagaa gagaatgatg ataaagcatc aaaggatgag tcgaaagata
     8521 aggatgacaa agcatctcaa gataaatcag atgataatca gaagaaaact gatgataata
     8581 aacaaccagc tcagcctaaa ccacagccgc aacaaccaac accaaagcca aataataatc
     8641 aacaaaacaa tcaatcaaat caacaagcaa aaccacaagc accacaacaa aatagccaat
     8701 caacaacaaa taaacaaaat aatgctaatg ataagtagta tttagtcaaa caaaaatgaa
     8761 ccagtatgac agacaacaca attaattagg ttgtctcgaa tattggttct tatttttata
     8821 attgttaatt aggggagaga tgatacttaa atagttagtt gtttattttg aagatagtga
     8881 aatttatttt gagtgaggtg ggacagaaat gatattttcg caaaatttat ttcgtcgtcc
     8941 caccccaact tccattgcct gtagaaattg gaaatccaat ttctctatgt tggggcccac
     9001 cccaacttgc acattattgt aagctgactt tccgccagct tctatgttgg ggcccctgac
     9061 tttaattgaa aaatgcttgt tacaagtgca ttttcgttcg gtcaactact actaatgtaa
     9121 ctttttggat tctagagcat tgatttatgt cttagttcaa ataataagca aatgattagc
     9181 gagtcagtat aagggtatac atttgactac agcgaataaa ataaaacgtt ttgttgaata
     9241 acaaatcgaa aatgtattgc aagcgcttta tcaaattatt tagaaaattt aagttttatg
     9301 cttgcaattt ttgaaataga atagtactat tgcaagtgta aagaggttaa tttttgtccc
     9361 acgcgggact taaaaaggca accactggtt gtgacatatc cttatttaca tttataaata
     9421 taaggaggag gtagtagtga aagacttatt gcaagcacag caaaagctta taccggatct
     9481 catagataaa atgtataaac gtttttctat tcttactact atctcaaaaa atcagcctgt
     9541 cggacgtcga agtttaagcg aacatatgga tatgactgaa cgtgtactgc gttctgaaac
     9601 agatatgctt aagaaacaag atttgataaa agttaagcct accggaatgg aaattacagc
     9661 tgaaggtgag caactgattt cgcaattgaa aggttacttt gatatctatg cagatgataa
     9721 tcgtctgtca gaaggtatta agaataaatt tcaaattaag gaagttcatg ttgttcctgg
     9781 tgatgctgat aatagtcaat ctgttaaaac agaattaggt agacaagcag gtcaattact
     9841 tgaaggcata ttacaagaag acgcgatagt tgctgtaact ggcggatcca cgatggcatg
     9901 tgttagtgaa gcaattcatt tattaccata taatgtattc ttcgtaccag ccagaggtgg
     9961 actaggcgaa aatgttgtct ttcaggcaaa cacaattgca gccagtatgg cacaacaagc
    10021 tggcggttat tatacgacga tgtatgtacc tgataatgtc agtgaaacaa catataacac
    10081 attgttgtta gagccatcag tcataaacac tttagacaaa attaaacaag caaacgttat
    10141 attacacggc attggtgatg cgctgaagat ggcgcatcga cgtcaatcac ctgaaaaggt
    10201 cattgaacaa cttcaacatc atcaagctgt cggagaggca tttggttatt attttgatac
    10261 acaaggtcaa attgtccata aggttaaaac aattggactt caattagaag accttgaatc
    10321 aaaagacttt atttttgcag ttgcaggagg caaatcgaaa ggtgaagcaa ttaaagcata
    10381 cttgacgatt gcacccaaga atacagtgtt aatcactgat gaagccgcag caaagataat
    10441 acttgaataa gagataaaaa gtttaatact ttttaaatat cattttaaag gaggccatta
    10501 taatggcagt aaaagtagca attaatggtt ttggtagaat tggtcgttta gcattcagaa
    10561 gaattcaaga agtagaaggt cttgaagttg tagcagtaaa cgacttaaca gatgacgaca
    10621 tgttagcgca tttattaaaa tatgacacta tgcaaggtcg tttcacaggt gaagtagagg
    10681 tagttgatgg tggtttccgc gtaaatggta aagaagttaa atcattcagt gaaccagatg
    10741 caagcaaatt accttggaaa gacttaaata tcgatgtagt attagaatgt actggtttct
    10801 acactgataa agataaagca caagctcata ttgaagcagg cgctaaaaaa gtattaatct
    10861 cagcaccagc tactggtgac ttaaaaacaa tcgtattcaa cactaaccac caagagttag
    10921 acggttctga aacagttgtt tcaggtgctt catgtactac aaactcatta gcaccagttg
    10981 ctaaagtttt aaacgatgac tttggtttag ttgaaggttt aatgactaca attcacgctt
    11041 acacaggtga tcaaaataca caagacgcac ctcacagaaa aggtgacaaa cgtcgtgctc
    11101 gtgcagcggc agaaaacatc atccctaact caacaggtgc tgctaaagct atcggtaaag
    11161 ttattcctga aatcgatggt aaattagatg gtggtgcaca acgtgttcct gtagctacag
    11221 gttcattaac tgaattaaca gtagtattag aaaaacaaga cgtaacagtt gaacaagtta
    11281 acgaagctat gaaaaatgct tcaaacgaat cattcggtta cactgaagac gaaatcgttt
    11341 cttcagacgt tgtaggtatg acttacggtt cattattcga cgctacacaa actcgtgtaa
    11401 tgtcagttgg cgaccgtcaa ttagttaaag ttgcagcttg gtatgataac gaaatgtcat
    11461 atactgcaca attagttcgt acattagcat acttagctga actttctaaa taattttagt
    11521 atagttttta ttcaaatacg ctagtgctca gaactattta gcattaatta aagcttatga
    11581 gtaagcgggg agcacaaacg cttctccgct tatttttata taaaatttcc taattacaag
    11641 gaggaaacac catggctaaa aaaattgttt ctgatttaga tcttaaaggt aaaacagtcc
    11701 tagtacgtgc tgattttaac gtacctttaa aagacggtga aattactaat gacaaccgta
    11761 tcgttcaagc tttacctaca attcaataca tcatcgaaca aggtggtaaa atcgtactat
    11821 tttcacattt aggtaaagta aaagaagaaa gtgataaagc aaaattaact ttacgcccag
    11881 ttgctgaaga cttatctaag aaattagata aagaagttgt tttcgtacca gaaacacgcg
    11941 gcgaaaaact tgaagctgct attaaagacc ttaaagaagg cgacgtatta ttagttgaaa
    12001 atacacgtta tgaagattta gacggtaaaa aagaatctaa aaatgatcca gaattaggta
    12061 aatactgggc atctttaggt gatgtgtttg taaatgatgc ttttggtact gcgcatcgtg
    12121 agcatgcatc taatgttggt atttctacac atttagaaac tgcagctgga ttcttaatgg
    12181 ataaagaaat taagtttatc ggtggcgtag ttaacgatcc acataaacca gttgttgcta
    12241 ttttaggtgg agcaaaagta tctgacaaaa ttaatgtcat caaaaactta gttaacatag
    12301 ctgataaaat tatcatcggc ggaggtatgg cttatacttt cttaaaagcg caaggtaaag
    12361 aaattggtat ttcattatta gaagaagata aaatcgactt cgcaaaagat ttattagaaa
    12421 aacatggtga taaaattgta ttaccagtag acactaaagt tgctaaagaa ttttctaatg
    12481 atgccaaaat tactgtagta ccatctgatt caattccagc agaccaagaa ggtatggata
    12541 ttggaccaaa cactgtaaaa ttatttgcag atgaattaga aggtgcgcac actgttgtat
    12601 ggaatggacc tatgggtgta ttcgagttca gtaactttgc acaaggtaca attggtgtat
    12661 gtaaagcaat tgcaaacctt aaagatgcaa ttacgattat cggtggcggt gattcagctg
    12721 cagcagcaat ctctttaggt tttgaaaatg acttcactca tatttcaact ggtggcggtg
    12781 cgtcattaga gtacctagaa ggtaaagaat tgcctggtat caaagcaatc gataataaat
    12841 aataaagtga tagtttaaag tgatgtggca tgtttgttta acattgttac gggaaaacag
    12901 tcacaagatg acatcgtgtt tcatcacttt tcaaaaatat ttacaaaaca aggagtgtct
    12961 ttaatgagaa caccaattat agctggtaac tggaaaatga acaaaacagt acaagaagca
    13021 aaagacttcg tcaatgcatt gccaacatta ccagattcaa aagaagtaga atcagtaatt
    13081 tgtgcaccag caattcaatt agatgcatta actactgcag ttaaagaagg aaaagcacaa
    13141 ggtttagaaa tcggtgctca aaatacgtat ttcgaagata atggtgcgtt cacaggtgaa
    13201 acgtctccag ttgcattagc agatttaggc gttaaatacg ttgttatcgg tcattctgaa
    13261 cgtcgtgaat tattccacga aacagataaa gaaattaaca aaaaagcgca cgctattttc
    13321 aaacatggaa tgactccaat tatatgtgtt ggtgaaacag acgaagagcg tgaaagtggt
    13381 aaagctaacg atgttgtagg tgagcaagtt aagaaagctg ttgcaggttt atctgaaaat
    13441 caactaaaat cagttgtaat tgcttatgaa ccaatctggg caatcggaac tggtaaatca
    13501 tcaacatctg aagatgcgaa tgaaatgtgt gcatttgtac gtcaaactat tgctgactta
    13561 tcaagcaaag aagtatcaga agcaactcgt attcaatatg gtggtagtgt taaacctaac
    13621 aacattaaag aatacatggc acaaactgat attgatgggg cattagtagg tggcgcatca
    13681 cttaaagttg aagatttcgt acaattgtta gaaggtgcaa aataatcatg gctaagaaac
    13741 caactgcgtt aattatttta gatggttttg cgaaccgcga aagcgaacat ggtaatgcgg
    13801 taaaattagc aaacaagcct aattttgatc gttattacaa caaatatcca acgactcaaa
    13861 tcgaagcgag tggcttagat gttggactac ctgaaggaca aatgggtaac tcagaagttg
    13921 gtcatatgaa tatcggtgca ggacgtatcg tttatcaaag tttaactcga atcaataaat
    13981 caattgaaga cggtgatttc tttgaaaatg atgttttaaa taatgcaatt gcacacgtaa
    14041 attcacatga ttcagcgtta cacatctttg gtttattgtc tgacggtggt gtgcacagtc
    14101 attacaaaca tttatttgct ttgttagaac ttgctaaaaa acaaggtgtt gaaaaagttt
    14161 acgtacacgc atttttagat ggtcgtgacg tagatcaaaa atccgctttg aaatacatcg
    14221 aagagactga agctaaattc aatgaattag gcattggtca atttgcatct gtgtctggtc
    14281 gttattatgc aatggaccgt gacaaacgtt gggaacgtga agaaaaagct tacaatgcta
    14341 ttcgtaattt tgatgcccca acttatgcaa ctgccaaaga aggtgtagaa gcaagctata
    14401 atgagggctt aactgacgaa ttcgtagtac cattcatcgt tgagaatcaa aatgacggtg
    14461 ttaatgatgg agatgcagtg atcttctata atttccgacc tgatagagca gcacaattat
    14521 cggaaatttt tgcgaacaga gcattcgaag gctttaaagt tgaacaagtt aaagacttat
    14581 tctatgcaac attcactaag tataacgaca atatcgatgc ggctatcgtt ttcgaaaaag
    14641 ttgatttaaa taatacaatt ggtgaaattg cacaaaataa caatttaaca caattacgta
    14701 ttgcagaaac tgaaaaatat cctcacgtta cttactttat gagtggtgga cgtaacgagg
    14761 aatttaaagg tgaacgccgt cgtttaattg attcacctaa agttgcaacg tatgacttga
    14821 aaccagaaat gagtgcttat gaagttaaag atgcattatt agaagagtta aataaaggtg
    14881 acttggactt aattatttta aactttgcta accctgatat ggttggacat agtggtatgc
    14941 ttgagccgac aatcaaagca atcgaagcgg ttgatgaatg tttgggtgaa gtcgttgata
    15001 agattttaga catggacggt tatgcaatta ttactgctga ccatggtaac tctgatcaag
    15061 tattgacgga tgatgatcaa ccaatgacta cgcatacaac gaacccagta ccagtgattg
    15121 taacaaaaga aggcgttaca cttcgagaaa ctggtcgctt aggtgactta gcacctacat
    15181 tattagattt attaaatgta gaacaacctg aagacatgac aggtgaatct ttaattaaac
    15241 actaatattg taaaagatgt taagtaaacg cttaatgaca cttatttttt gaaaataata
    15301 gtaatatcat tttgttaaat gaaagaataa agctataata attatagaat aactatttaa
    15361 aggagattat aaacatgcca attattacag atgtttacgc tcgcgaagtc ttagactctc
    15421 gtggtaaccc aactgttgaa gtagaagtat taactgaaag tggcgcattt ggtcgtgcat
    15481 tagtaccatc aggtgcttca actggtgaac acgaagctgt tgaattacgt gatggagaca
    15541 aatcacgtta tttaggtaaa ggtgttacta aagcagttga aaacgttaat gaaatcatcg
    15601 caccagaaat tattgaaggt gaattttcag tattagatca agtatctatt gataaaatga
    15661 tgatcgcatt agacggtact ccaaacaaag gtaaattagg tgcaaatgct attttaggtg
    15721 tatctatcgc agtagcacgt gcagcagctg acttattagg tcaaccactt tacaaatatt
    15781 taggtggatt taatggtaag cagttaccag taccaatgat gaacatcgtt aatggtggtt
    15841 ctcactcaga tgctccaatt gcattccaag aattcatgat tttacctgta ggtgctacaa
    15901 cgttcaaaga atcattacgt tggggtactg aaattttcca caacttaaaa tcaattttaa
    15961 gcaaacgtgg tttagaaact gcagtaggtg acgaaggtgg tttcgctcct aaatttgaag
    16021 gtactgaaga tgctgttgaa acaattatcc aagcaatcga agcagctggt tacaaaccag
    16081 gtgaagaagt attcttagga tttgactgtg catcatcaga attctatgaa aatggtgtat
    16141 atgactacag taagttcgaa ggcgaacacg gtgcaaaacg tacagctgca gaacaagttg
    16201 actacttaga acaattagta gacaaatatc ctatcattac aattgaagac ggtatggacg
    16261 aaaacgactg ggatggttgg aaacaactta cagaacgtat cggtgaccgt gtacaattag
    16321 tgggtgacga tttattcgta acaaacactg aaattttagc aaaaggtatt gaaaacggaa
    16381 ttggtaactc aatcttaatt aaagttaacc aaatcggtac attaactgaa acatttgatg
    16441 caatcgaaat ggctcaaaaa gctggttaca cagcagtagt ttctcaccgt tcaggtgaaa
    16501 cagaagatac aacaattgct gatatcgctg ttgctacaaa cgctggtcaa attaaaactg
    16561 gttcattatc acgtactgac cgtattgcta aatacaatca attattacgt atcgaagatg
    16621 aattatttga aactgctaaa tatgacggta tcaaatcatt ctacaactta gataaataat
    16681 cattttaaac attaagtact gccataattt tagttgagga ttattatgtc ggtataaatt
    16741 gaataaagtt tttgagttca cgcttaaaaa gttcacgctt gtatttataa cctgccacag
    16801 agttgagact gtggtaggtt ttttattttg gatggcttca aatcatgatt tataaggggc
    16861 ttgataaatc atgaggtaac taataacaca tatttaactt gtattcttaa actggtataa
    16921 taaatttatg ttgaaatgaa tattgtatga cagggtattc acttttatta agaggtaaaa
    16981 ttaaataaag gttttataga acgtatttaa atatatgagg agtaaacaaa tggctgatag
    17041 aacgaataaa gaaattaaaa caggacgctt tattgcaact gcatcaatcg tattctcaat
    17101 attattgatt attcattact ttgtttcgtt ggataatgcg actgccaaag cattacttaa
    17161 tttaacgaat caaaacactt cagataaagc gattgattac attttaaaca gctttagatt
    17221 cactggtatt atgtatattt tggcttatct agcaggcttc atcacttttt ggaatcgaca
    17281 tacttatgtg tggtggttta tgtttgcagt ttatgtatca aatagtttgt ttacgttgat
    17341 taatttatca atcacaattc aagcaataaa agctgcacac ggtgcgtact taacattgcc
    17401 aattttaatt gttattatag gttcggttgc attagcgatt tatatgcttg ttgtttcaat
    17461 taagcgtaaa agtacattta atcgctagaa aattgatttt aacaataaaa atatgatata
    17521 ctacttgtcg tatataagga acggaggaca atttatgcat acatttttaa tcgtattatt
    17581 aatcattgat tgtattgcat taataactgt tgtactactc caagaaggta aaagcagtgg
    17641 actttcaggt gccatcagtg gtggtgctga gcagttattc ggtaaacaaa aacaacgtgg
    17701 cgtcgattta ttcttaaata gattaacaat tattttatca atattatttt ttgtacttat
    17761 gatttgcata agttatcttg gtatgtaagg tccggcgatg taaatgtcgg gcttttttat
    17821 ttataattaa gaatgtaata gtttaacaat aagctatgta aaatatatag cctagttaag
    17881 tatgcaaagg gagcgttaga tttatgcaga taaaattacc aaaacctttc ttttttgaag
    17941 aaggcaaacg tgccgtgtta ttattacatg gatttacagg caattcgtct gatgtacgtc
    18001 aattaggtcg atttttacaa aaaaagggat atacttcgta tgcaccgcaa tatgaaggtc
    18061 atgcagcacc accagaagag atacttcaat caagtccttt tgtttggttt aaagatgctt
    18121 tagatggcta tgactacctt gttgaacaag gttatgatga aatcgttgtt gctggcttgt
    18181 cattaggtgg cgattttgca ttaaaattaa gcttaaatag agatgtaaag ggtattgtaa
    18241 cgatgtgtgc acctatgggt ggcaaaactg aaggtgccat ttatgaaggc tttttagaat
    18301 atgcacgcaa ttttaaaaag tatgaaggta aagatcaaga aacgattgac aacgaaatgc
    18361 atcattttaa accaactgaa actttaaaag aattaagtga agcattagat acgattaaag
    18421 atcaagttga tgaggtgttt gacccaattt tagtgattca agcagaaaac gacaatatga
    18481 ttgatccaca atccgcaaat tatatatatg aacatgtaga ctctgacgat aaaaatatca
    18541 agtggtacag tgaatctgga catgttatta cgattgataa agaaaaagaa caagtatttg
    18601 aagatattta tcaattttta gagtcattag attggtcaga ataaaaagag attttaacat
    18661 tagaaaggag gggcataatg aatttaaagc aatcaataga agagattatt aatcaacctg
    18721 aatatgaacc tatgtcagtg tcagattttc aagatgcatt aggtttaagt agtgccgact
    18781 catttagaga tttaattaag gtgctcgtag aattagaaca atcaggatta attgaacgta
    18841 caaaaacaga cagatatcaa aaaaagcata gttcaaaagg tcattcaaaa ttgataaagg
    18901 gaacgttaag tcaaaataaa aaaggctttg cattcttaag acctgaagat gaggatatgg
    18961 aagatatatt tattccccca acaaaaatta atcgtgcctt agatggagat actgtaattg
    19021 tagaaataca tcaatctaaa ggtgaacata aaggtaaaat tgaaggggaa gttaagtcga
    19081 ttgagaagca ttcagtaact caagttgttg gtacgtatag tgaagctaga catttcggat
    19141 ttgttattcc agatgataaa cgtattatgc aagatatttt cattcctaaa ggacaaagtt
    19201 taggtgcagt agatgggcat aaggtacttg ttcaaattac taaatatgct gatggttcag
    19261 ataatccaga aggacatatt tctgctattt taggacataa aaatgatcct ggcgtagata
    19321 ttttatctat tatctatcaa catggcatag aaattgaatt tcctgatgaa gtgttacaag
    19381 aagctgaagc agtacctgat catattgaga atactgaaat taaaggccgt catgatttac
    19441 gtgatgaatt gacaatcaca attgatggtg ctgatgctaa agacttagat gacgcaatta
    19501 gtgttaaaaa gttagcgaac ggtaatacgc aattaactgt aagtattgct gatgtcagtt
    19561 attatgtaac tgaagattca gctttagata aagaagctta tgatagagcg acaagtgtat
    19621 atcttgttga ccgtgtgatc ccgatgattc cacatcgatt aagcaatggt atttgttcat
    19681 taaatcctca cgttgatcgt ttaacgttaa gctgtcgcat ggaaattgat gctagtggtc
    19741 gcgttgttaa acatgaaatt tttgatagtg ttatacattc tgattatcga atgacgtatg
    19801 atgcggtaaa tcagattatt actgaaaagg atcctgcgat tcgagagcag tataatgaaa
    19861 ttacgcctat gctagattta gcacaagatt tatctaatcg tttgattcaa atgagaaaac
    19921 gacgtggtga aatcgatttt gatattagtg aagcaaaagt attagttaac gaagatggta
    19981 tacctacaga tgttcaatta agacaacgtg gcgagggtga acgtctaatt gaatcattta
    20041 tgttaattgc aaatgaaaca gttgctgaac attttagtaa gttagatgta ccttttattt
    20101 accgagtgca tgagcaacct aaatcagatc gcttaagaca attctttgat tttattacaa
    20161 actttggcat catgattaag ggtactggcg aagatattca tccaacaaca cttcaaaagg
    20221 ttcaagaaga agtagaaggt cgacctgaac aaatggtcat ttcaacaatg atgttacgtt
    20281 caatgcaaca agcgcattat gatgatgtga acttgggaca ttttggctta tcagctgaat
    20341 attatacgca ttttacatca ccaattagac gttatcctga tttaacagtt catcgtttaa
    20401 tccgtaagta tttaattgag aaatcaatgg ataacaaaga agtgaagcgt tgggaagaca
    20461 aattgcctga gttagctgaa catacttcta aacgtgaacg tcgtgctatt gaggcagaac
    20521 gtgatactga tgaattgaaa aaagcagaat atatgattca acatattggt gatgaatttg
    20581 aaggtattgt cagctcagta gctaacttcg gtatgttcat tgaattgcca aatacgatag
    20641 aaggtatggt tcatattgcg aatatgactg atgattatta ccgttttgaa gagcgtcaaa
    20701 tggcattaat tggtgagcgt caagctaaag tatttagaat tggtgacaca attaaggtta
    20761 aagtgacgca tgttgatgta gatgaacgat taattgattt tcaaattgta ggtatgcctt
    20821 taccgaaaaa tgatcgatca cagcgcccag cgcgaggtaa gacaattcaa gctaaaacgc
    20881 gtggtaaatc attagataaa tcgaaatctg atgataaagg tcataagaaa aaaggtaagc
    20941 aacgtaaagg taaaaatgaa cgtaaaaatg acaaatcagg aaacagtaaa cataagccat
    21001 tttataaaga taaaagtgtg aaaaagaaag cacgtcgtaa gaaaaaataa gcagcaatga
    21061 ggtgagtatg aatggctaag aagaaatcac caggtacatt agcggaaaat cgtaaagcta
    21121 gacatgatta taatattgaa gacacgattg aagcgggaat tgtattgcaa ggcacagaaa
    21181 taaaatcaat tcgccgaggt agtgctaacc ttaaagatag ttatgcgcaa gttaaaaacg
    21241 gtgaaatgta tttgaataat atgcatatag caccatacga agaagggaat cgttttaatc
    21301 acgatcctct tcgttctcga aaattattat tgcacaagcg tgaaatcatt aaattgggtg
    21361 atcaaacacg tgagattggt tattcgattg tgccgttaaa gctttatttg aagcatggac
    21421 attgtaaagt attacttggt gttgcacgag gtaagaaaaa atatgataaa cgtcaagctt
    21481 tgaaagaaaa agcagtcaaa cgagatgttg cgcgcgatat gaaagcccgt tattaagcga
    21541 tttagttgct taatcgggct atatttgata tagttatatg tgcttttgta aattacaaaa
    21601 gtatgatttg tttgatttat tatttcgggg acgttcatgg attcgacagg ggtcccccga
    21661 gctcattaag cgtgtcggag ggttgtcttc gtcatcaaca cacacagttt ataataactg
    21721 gcaaatcaaa caataatttc gcagtagctg cctaatcgca ctctgcatcg cctaacagca
    21781 tttcctatgt gctgttaacg cgattcaacc ttaataggat atgctaaaca ctgccgtttg
    21841 aagtctgttt agaagaaact taatcaatct agcatcatgt tggttgttta tcacttttca
    21901 tgatgcgaaa cctttcgata aactacacac gtagaaagat gtgtatcagg acctctggac
    21961 gcgggttcaa atcccgccgt ctccataaaa atatgcttgg aaaccttgat ttaatggggt
    22021 tttaatctag caagtgtcaa atatgtgtca agaaaataat tttctgacac gttgaccttg
    22081 ctctttttta tgttttttat cttttcttgt gaggaaggtt aaattagctg tgtactttga
    22141 tatgatagta tctgcttagg ttcaaatttt catgaatgaa aaattaatag ttgtgtacat
    22201 ataagatttt gcgttaaggg ttgaaagaat atgtgtcaaa taagtgtcaa aaaagttgag
    22261 ctttgagttt taatattgtt cataaaaaaa attctttata taagataatt cttcattc
//
LOCUS       DAHHOJ010000039        21478 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.039, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000039 DAHHOJ010000000
VERSION     DAHHOJ010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21478
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.039"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(25..291)
                     /locus_tag="NBH32_001247"
     CDS             complement(25..291)
                     /locus_tag="NBH32_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HCD1514863.1"
                     /translation="MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALE
                     GIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDKHIKIASCKYHY"
     gene            complement(291..542)
                     /locus_tag="NBH32_001248"
     CDS             complement(291..542)
                     /locus_tag="NBH32_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HCD1514864.1"
                     /translation="MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESD
                     YNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKDIDV"
     gene            complement(705..806)
                     /locus_tag="NBH32_001249"
                     /pseudo
     CDS             complement(705..806)
                     /locus_tag="NBH32_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501155.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(813..1412)
                     /locus_tag="NBH32_001250"
     CDS             complement(813..1412)
                     /locus_tag="NBH32_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HCD1514865.1"
                     /translation="MTKKLLTLFIVSMLILTACGKKESATTSSKNGKPLVVIYGDYKC
                     PYCKELDEKVMPKLRKNYIDNHKVEYQFVNLAFLGKDSIVGSRASHAVLMYAPKSFLD
                     FQKQLFAAQQDENKEWLTKELLDKHIKQLHLDKETENKIIKDYKTKDSKSWKAAEKDK
                     KIAKDNHIKTTPTAFINGEKVEDPYDYESYEKLLKDKIK"
     gene            complement(1431..1793)
                     /locus_tag="NBH32_001251"
     CDS             complement(1431..1793)
                     /locus_tag="NBH32_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000819849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD1514866.1"
                     /translation="MKRFVATVLLLLVFISGCGNDKYVKEIDEAVKIQNQKQEQLAKK
                     GNGDRVDHFERKDANIYVYDKDKIIILAYKPLSNDDEVHYYAYDFSDKRVSYKQDFDS
                     RRYYQQHDADYHEENMTN"
     gene            2048..3298
                     /gene="fmhA"
                     /locus_tag="NBH32_001252"
     CDS             2048..3298
                     /gene="fmhA"
                     /locus_tag="NBH32_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FemA/FemB family glycyltransferase FmhA"
                     /protein_id="HCD1514867.1"
                     /translation="MNFVTLTSDEFNAFTTKHFSHYTQSAIHYNHRVDLKGDVHLVGV
                     KDDNGQVIAGCLLTEARTLKFFKYFYTHRGPVMDYTNQSLVAFFFKALTSYLKKQNCL
                     YVLVDPYLIENLRNADGEIVKSYDNRAFVRTMDKLGYKHQGFPVGYDSMSQIRWLSVL
                     DLKDKTEDQLLKEMDYQTRRNIKKTYDIGVKTKTLTIDETQTFFDLFHMAEEKHGFKF
                     RELPYFEEMQKLYGDHAMLKLAYIDLNDYLKTLQLKQQQLAAELSGVEEALEESPNSK
                     KNKTKRTQLEQQLSSNKRKIDNTIEQIEQDGAVLNLASALFIYNEHEVYYLSSGSNPK
                     YNAYMGAYHLQWEMIKFAKAHHIDRYNFYGITGDFSESSEDYGVQQFKKGFNAHVEEY
                     IGDFIKPIKPMLYKLQTYLKHKRR"
     gene            complement(3383..4114)
                     /locus_tag="NBH32_001253"
     CDS             complement(3383..4114)
                     /locus_tag="NBH32_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1514868.1"
                     /translation="MIQLNNIHKSFNDVEVIKGIDLSVDKGEVVTLIGRSGSGKTTLL
                     RMINALEIPTEGTVFVNGKTYTSKDKKSQIEVRKQSGMVFQSYNLFPHKTALENVMEG
                     LITVKKLKKAEAREKSLELLEKVGLTHVKDQRPHALSGGQQQRVAIARALAMNPKVML
                     FDEPTSALDPELVNDVLKVIKDLANEGMTMVIVTHEMRFAREVSNNIVFIHEGVIGEQ
                     GAPEEIFNNPKTEELKRFLNVINEE"
     gene            complement(4111..4830)
                     /locus_tag="NBH32_001254"
     CDS             complement(4111..4830)
                     /locus_tag="NBH32_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCD1514869.1"
                     /translation="MFLNLNSEQLHALDAAKQAFGPMLEGLVKYSIPITLVTFVLGLI
                     IALFTALMRISTSKVLRSIARVYVSIIRGTPMIVQLFIIFYGIPELGRLLTNDADNQW
                     TLAPVVAAIIGLSLNVGAYASEIIRGGIISIPKGQTEAAYSIGMTYRQTIQRIILPQA
                     IRVSIPALGNTFLSLIKDTSLLGFILVAEMFRKAQEVASTTYEYLTIYVLVALMYWVV
                     CFIISIIQGIYESYIERGYRS"
     gene            complement(4811..5590)
                     /locus_tag="NBH32_001255"
     CDS             complement(4811..5590)
                     /locus_tag="NBH32_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HCD1514870.1"
                     /translation="MKRLLFVVIAFVFILAACGNNSSKDKEASKDSKTINVGTEGTYA
                     PFSFHDKDGKLTGYDIDVIKAVAKEEGLKLKFNETSWDSMFAGLDAGRFDVIANQVGI
                     NPDREKKYKFSKPYTFSSAVLVIRENEKDIKDFDDVKGKKLAQTFTSNYGKLAKDKGA
                     DITKVDGFNQSMDLLLSKRVDGTFNDSLSYLDYKKQKPNAKIKAIKGNAEQSKSAFAF
                     SKKVDDETVQKFNDGLKKIEENGELAKIGKKWFGQDVSKSK"
     gene            complement(5711..7150)
                     /gene="mdeA"
                     /locus_tag="NBH32_001256"
     CDS             complement(5711..7150)
                     /gene="mdeA"
                     /locus_tag="NBH32_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000038968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdeA"
                     /protein_id="HCD1514871.1"
                     /translation="MSKKQKLTMIITMLMGGFFGLLNETLLVTALPSIMKDFEISYTQ
                     VQWLTTAFLLTNGIVIPLSALVIQRYTTRQVFLVGISIFFLGTLLGGLSPHFATLLVA
                     RIIQALGAGIMMPLMMTTILDIFQPHERGKYMGIFGLVIGLAPAIGPTLSGYLVEYFN
                     WRSLFHVVAPIAAVTFLIGFKTIKNVGTTIKVPIDFISVIFSVLGFGGLLYGTSSISE
                     KGFDNPIVLVSMIGGVVLVALFVLRQYRLSTPLLNFAVFKNKQFTVGIIIMGVTMVSM
                     IGSETILPIFVQNLLHRSALDSGLTLLPGAIVMAFMSMTSGALYEKFGPRKLALVGMA
                     IVIITTGYFVVMDEQTSTIMLATVYAIRMVGIALGLIPVMTHTMNQLKPEMNAHGSSM
                     TNTVQQIAGSIGTAALITILSHASKNFSPTMSDYNGMNKIEMMNQIKVDTMLHGYHAG
                     FLFALLITVISFFCSFMLQGKKKEVDSHQ"
     gene            7324..7428
                     /locus_tag="NBH32_001257"
     CDS             7324..7428
                     /locus_tag="NBH32_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HCD1514872.1"
                     /translation="MKLDLQTARRNLNSPNIKTRKRALKIIKQHKRAK"
     gene            complement(7582..8268)
                     /locus_tag="NBH32_001258"
     CDS             complement(7582..8268)
                     /locus_tag="NBH32_001258"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCD1514873.1"
                     /translation="MPKLILCRHGQSEWNAKNLFTGWEDVNLSEQGINEATRAGEKVR
                     ENNIAIDVAFTSLLTRALDTTHYILTESKQQWIPVYKSWRLNERHYGGLQGLNKDDAR
                     KEFGEEQVHIWRRSYDVKPPAETEEQREAYLADRRYNHLDKRMMPYSESLKDTLVRVI
                     PFWTDHISQYLLDGQTVLVSAHGNSIRALIKYLEDVSDEDIINYEIKTGAPLVYELTD
                     DLEVIDKYYL"
     gene            complement(8595..9461)
                     /locus_tag="NBH32_001259"
     CDS             complement(8595..9461)
                     /locus_tag="NBH32_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HCD1514874.1"
                     /translation="MSHNENLKLAQRGAYLSLIVYIILSIVKYVTGFVFNSAAVRADA
                     LNNMTDIIVSLAVIIGLKISIKPADRNHPYGHLKSENISSLLVSFVIMFVGIQVVIQN
                     APRLFKEDDVVPNAITIIVSLISGLVMLIVFAVNQRLAKRTKSSSLNSAAKDNLSDSL
                     VSIGTAIGLIFTQIGFPIVDIILATLLGLLIVYTGFGIFKEAIFMLSDGFNETELEAY
                     RNDILEVDEVQEVKSIKGRYHGSSVFIDVTIVVDANLSLVAAHQICDNVEHHLHKKGI
                     SSVYVHPEPDHL"
     gene            complement(9960..10064)
                     /locus_tag="NBH32_001260"
     CDS             complement(9960..10064)
                     /locus_tag="NBH32_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514875.1"
                     /translation="MLTNRLTSVNYVKKRLIQNIQRILSKEVLYGEMQ"
     gene            10159..11469
                     /locus_tag="NBH32_001261"
     CDS             10159..11469
                     /locus_tag="NBH32_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B domain-containing protein"
                     /protein_id="HCD1514876.1"
                     /translation="MKNKYISKLLVGAATITLATMISNGEAKASENTQQTSTKHQTTQ
                     NNYVTDQQKAFYQVLHLKGITEEQRNQYIKTLREHPERAQEVFSESLKDSKNPDRRVA
                     QQNAFYNVLKNDNLTEQEKNNYIAQIKENPDRSQQVWVESVQSSKAKERQNIENADKA
                     IKDFQDNKAPHDKSAAYEANSKLPKDLRDKNNRFVEKVSIEKAIVRHDERVKSANDAI
                     TKLQKNDSIENRRLAQREVNKAPMDVQKHLQKQLDALVAQKDAEKKVAPKVEAPQIQS
                     PQIEKPKAESPKVEVPQIQSPKVEVPQSKLLGYYQSLKDSFNYGYKYLTDTYKSYKEK
                     YDTAKYYTDKYFKYKGTIDKTVLSVFGNGYKSYIQPLKVEDQKNYVSKSYAQVRNYVT
                     ETLNTGKVLYAFYQNPKVVKAAITTAETATSIKNIFSSFTSFFK"
     gene            11960..12889
                     /gene="hlgA"
                     /locus_tag="NBH32_001262"
     CDS             11960..12889
                     /gene="hlgA"
                     /locus_tag="NBH32_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB subunit A"
                     /protein_id="HCD1514877.1"
                     /translation="MIKNKILTATLAVGLIAPLANPFIEISKAENKIEDIGQGAEIIK
                     RTQDITSKRLAITQNIQFDFVKDKKYNKDALVVKMQGFISSRTTYSDLKKYPYIKRMI
                     WPFQYNISLKTKDSNVDLINYLPKNKIDSADVSQKLGYNIGGNFQSAPSIGGSGSFNY
                     SKTISYNQKNYVTEVESQNSKGVKWGVKANSFVTPNGQVSAYDQYLFAQDPTGPAARD
                     YFVPDNQLPPLIQSGFNPSFITTLSHEKGKGDKSEFEITYGRNMDATYAYVTRHRLAV
                     DRKHDAFKNRNVTVKYEVNWKTHEVKIKSITPK"
     gene            13457..14404
                     /gene="hlgC"
                     /locus_tag="NBH32_001263"
     CDS             13457..14404
                     /gene="hlgC"
                     /locus_tag="NBH32_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgCB subunit C"
                     /protein_id="HCD1514878.1"
                     /translation="MLKNKILATTISVSLLAPLANPLLENAKAANDTEDIGKGNDVEI
                     IKRTEDKTSNKWGVTQNIQFDFVKDKKYNKDALILKMQGFISSRTTYYNYKNTNHIKS
                     MRWPFQYNIGLKTNDPNVSLINYLPKNKIESTNVSQTLGYNIGGNFQSAPSLGGNGSF
                     NYSKSISYTQQNYVSEVEQQNSKSVLWGVKANSFVTASGQKSAFDSDLFVGYKPNSKD
                     PRDYFVPDSELPPLVQSGFNPSFIATVSHEKGSGDTSEFEITYGRNMDVTHAIKRSTH
                     YGNSYLDGHRVHNAFKNRNYTVKYEVNWKTHEIKVKGQN"
     gene            14406..15383
                     /gene="hlgB"
                     /locus_tag="NBH32_001264"
     CDS             14406..15383
                     /gene="hlgB"
                     /locus_tag="NBH32_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component gamma-hemolysin HlgAB/HlgCB subunit
                     B"
                     /protein_id="HCD1514879.1"
                     /translation="MNMNKLVKSSVATSMALLLLSGTANAEGKITPVSVKKVDDKVTL
                     YKTTATADSDKFKISQILTFNFIKDKSYDKDTLVLKAAGNINSGYERPNPKDYDFSKI
                     YWGAKYNVSISSQSNDSVNVVDYAPKNQNEEFQVQNTLGYTFGGDISISNGLSGGLNG
                     NTAFSETINYKQESYRTTLSRNTNYKNVGWGVEAHKIMNNGWGPYGRDSFHPTYGNEI
                     FLAGRQSSSYAGQNFIAQHQMPLLSRSNFNPEFLSVLSHRQDGAKKSKITVTYQREMD
                     LYQIRWNGFYWAGANYKNFKTRTFKSTYEIDWENHKVKLLGTKETENNK"
     gene            complement(15435..15902)
                     /locus_tag="NBH32_001265"
     CDS             complement(15435..15902)
                     /locus_tag="NBH32_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HCD1514880.1"
                     /translation="MTSKDITQISVIAAILTILAVLKIPSIIPGLDFQLSAPAALLIL
                     AFFGIKKYFLGGLLSSLLLLVFGVFNPINVIISIIFRVIAIAVVYLLKINVLSLVLAS
                     VLGSLVSRLLLSIILNLPVWVVLLNAIPGVIFTLIVAIPLYLTLRKRMAVLLR"
     gene            complement(15913..16605)
                     /locus_tag="NBH32_001266"
     CDS             complement(15913..16605)
                     /locus_tag="NBH32_001266"
                     /EC_number="6.2.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxyhexanoate--CoA ligase"
                     /protein_id="HCD1514881.1"
                     /translation="MYSIKMRSSNQDVHISGAETICEFDKIEQTVRRFYNKGFFHENG
                     QPDFLNIKIQKIMEPIQQIKALQITEDDKANLQHLTQECGVTEQALNQGMTYIKNETV
                     YTGAIILSAISGKRLDSFGQRGIRATHFSFEDINNKGDLNERVTDALAIASCINAHPY
                     VKGELCVSDDLTYTTGYFASAKIGYHRLFDIKPVNTRYGGRIIFVDDRIDLNHYISFL
                     ESTPKQVVYETV"
     gene            complement(16616..17731)
                     /locus_tag="NBH32_001267"
     CDS             complement(16616..17731)
                     /locus_tag="NBH32_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HCD1514882.1"
                     /translation="MMFQEQIDLLQQKGLYRSLKSVGYIDRQYIEVENKRCTNYTSND
                     YLGLGQIAFDKDDFERFMRKYSYHLSSSRLISGSSTAYEEIETMLAGWLGYNACTILN
                     SGYDANLALFNIFKNTNCVVFSDQENHASIIDGIKLSGLEKVIYKHLDIADLEKRLAE
                     YPNQNIQKIIISDSVFSTNGDVVDIGQLVSLKHKYNATLILDVSHSFGIENYSNYQGI
                     DILTSSLSKACGAYGGVILSSNDVKDMLINHGRPLIYSSSLPIYNLYFIKRNIEKLIN
                     ADDRRTKLNSLIKYFNQKLKTLNVNYNSSNSPIKFIEFDDIEAAKNIHQTLLKHHVFT
                     SYLRYPTVTKPMLRISLSYFHTEQDVDRLFEILHQED"
     gene            complement(17709..18716)
                     /gene="bioB"
                     /locus_tag="NBH32_001268"
     CDS             complement(17709..18716)
                     /gene="bioB"
                     /locus_tag="NBH32_001268"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCD1514883.1"
                     /translation="MNLAKRILQGEQLTKETVLKIYEDTNIDTLDLLNEAYILRKHYF
                     GKKVKLNMILNAKSGICPENCGYCGQSRDIKQKQRYALIPEEQIIDGAKVAHDNHIGT
                     YCIVMSGRGPSDKEVDHISNTVRTIKSQHPQLKICACLGLTNDEQAKKLKSAGVDRYN
                     HNINTSENYHDNVVTTHSYKDRTDTIELMKANNISPCSGVICGMGESNQDIVDMAFAL
                     KEMDADSIPINFLHPIKGTKFGSMDDLTPMKCLRIVALFRLINPTKEIRIAGGREVNL
                     RSLQPLALKAANSIFVGDYLITGGQPNQLDYDMINDLGFEIDYDTCENKENKNDVSRA
                     N"
     gene            complement(18718..20076)
                     /gene="bioA"
                     /locus_tag="NBH32_001269"
     CDS             complement(18718..20076)
                     /gene="bioA"
                     /locus_tag="NBH32_001269"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCD1514884.1"
                     /translation="MNYTQQLKQKDSEYVWHPFTQMGVYSKEEAIIIEKGKGSYLYDT
                     NGNKYLDGYASLWVNVHGHNNKYLNKVIKKQLNKIAHSTLLGSSNIPSIELAEKLIEI
                     TPSNLRKVFYSDTGSASVEIAIKMAYQYWKNIDREKYAKKNKFITLNYGYHGDTIGAV
                     SVGGIKTFHKIFKDLIFENIQVESPSFYRSNYDTENEMMTAILTNIEQILIERNDEIA
                     GFILEPLIQGATGLFVHPKGFLKEVEKLCKKYDVLLICDEVAVGFGRTGKMFACNHED
                     VQPDIMCLGKAITGGYLPLAVTLTSQKIYNAFLSDSHGVNTFFHGHTYTGNQIVCTVA
                     LENIRLYEKRKLLSHIETTSSTLEKQLHALKRHRNVGDVRGRGLMFGVELVTDKDSKT
                     PLEIEKVERIVRNCKENGLMIRNLENVITFVPVLSMSNKEVKTMVRIFKKAVHNILDR
                     KC"
     gene            complement(20054..20740)
                     /gene="bioD"
                     /locus_tag="NBH32_001270"
     CDS             complement(20054..20740)
                     /gene="bioD"
                     /locus_tag="NBH32_001270"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dethiobiotin synthase"
                     /protein_id="HCD1514885.1"
                     /translation="MRIFITSTNTDVGKTYVTKHLYHALKTRGHRVCVFKPFQTEERQ
                     DGTFPDLEVFKNECDLSYDITSLYTFKQPVSPHLAFKMADQIFLNKQRVLDKVKVLDK
                     EFDFILIEGAGGIAVPIYEGTDDFYMTKDLINDCADCVISVLPSKLGAISDAIVHQDY
                     VNQNVSASNFLIMNRYTDSYIEKDNQMTIGKLTNKTVYTFEEHATYENFSEAFLKQLI
                     GVKNELHTTT"
     gene            complement(20795..20962)
                     /locus_tag="NBH32_001271"
     CDS             complement(20795..20962)
                     /locus_tag="NBH32_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514886.1"
                     /translation="MQLLILTDIFYRIAVYICPNSVRSLSHFNSKVKILPKFIVILRS
                     LNSCFLEKHAP"
     gene            complement(21128..21478)
                     /locus_tag="NBH32_001272"
                     /pseudo
     CDS             complement(21128..21478)
                     /locus_tag="NBH32_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         7456 a         3875 c         2987 g         7160 t
ORIGIN      
        1 atttttatag tgtgactttt agatttaata atggtattta catgatgcta tttttatatg
       61 cttgtcatct actgtataaa ccaatctatg ttcgtgattg atacgtctac tataataccc
      121 agtcagattc gattttaact tttcaggctt acctatacct tccaatgcac catttctgtc
      181 aatactttta agtaactcat taatcttctt caccattttt ttatcgttct gctgaaaata
      241 cttataatct tcaaaggctt gaggcgaaaa cgtaatattt aacctagcca ttatacatct
      301 atatctttcg ttatagtttt cccacgttct agatctgcaa ttgattgagc taagtgctca
      361 gcattatttg ggttctgttg gaggtaaagt gtttccatca tggagttata atctgattct
      421 gacatgatta ctacgttttt atcatcagta gatgttacag ttaccatatc actatcatca
      481 tttacttttg tcataagtgc ctttaaattc tgtcgagcgt atgaataatt tttaataatc
      541 atatttatcg cctcctttta acacacctca attgtacaaa tatctgtaca ataaaacaag
      601 cgacacaaaa aaattactaa tcaagatttt gcacggggaa gaatcaatac ctatcctgta
      661 ctgattccct actaaaaaat gtttacatga taatttccac ccatttagac ttcatacatt
      721 aaaaagacaa tctctatcat attgctaata gaaattgcct tttcatattg tcctaaaaaa
      781 ttggtatcac ttacttatct caacaattaa gactatttga ttttatcttt taataacttc
      841 tcataacttt cataatcata tggatcttca actttctcgc cattaataaa tgcagttggt
      901 gtcgttttta tatgattatc tttagctatt tttttatctt tctctgcagc tttccaagac
      961 ttgctatctt ttgtcttgta atcttttata attttatttt ccgtctcttt atctaaatgc
     1021 agttgtttaa tatgtttatc taatagttct tttgttaacc attctttatt ttcatcttgc
     1081 tgggcagcaa ataattgctt ttgaaaatct aaaaatgatt taggtgcata catcaatact
     1141 gcatgactcg cacgcgaacc aacaattgag tctttaccta agaaagctaa attgacaaat
     1201 tggtattcca ctttgtgatt atctatataa tttttacgca actttggcat gactttttca
     1261 tctaattctt tacaataagg acatttatag tcgccatata tgacaactaa tggtttgccg
     1321 tttttcgaag atgtcgttgc tgattctttt ttaccgcaag ctgttaaaat taacatgctc
     1381 actataaata atgttagtaa ttttttagtc ataataactc ctgttcatat ctagttcgtc
     1441 atattttctt catgataatc cgcatcatgt tgttgataat atcgtctcga atcaaaatct
     1501 tgcttatatg atacacgttt atcactaaaa tcatatgcat aataatgcac ttcatcatca
     1561 ttactcaaag gtttataagc taaaatgata attttatcct tatcatagac ataaatatta
     1621 gcatctttgc gttcaaaatg atcaacacga tcaccgttgc cttttttggc aagttgttct
     1681 tgtttttgat tttgaatttt aactgcttca tctatttctt tcacatattt atcattacca
     1741 catcctgata taaagactag taataataat accgtcgcca caaatcgctt caatgctccc
     1801 actcctacta tcaacttcca actaactttc aatataatta atactataca ccgtcaaaca
     1861 tcaatgcgct acaactacta ccaatgcatt taatataaat atccaaagaa ataacagtta
     1921 tgtatagtca aagaaaaata tatttcattt tctatgtgta tttacaatca gtttttgata
     1981 aaataaaaaa atagcattat aaataaattc aaaaattaca gttcaattca gagagggatg
     2041 ggataaaatg aactttgtaa cgttgacttc ggatgagttc aatgcgttta caacaaagca
     2101 tttttcacat tacacacaat cagctattca ttacaatcat agagttgatt taaaaggcga
     2161 tgtgcatctt gtaggggtta aagatgacaa tggtcaagtg attgcaggat gcttattgac
     2221 agaagcacgc acacttaaat ttttcaaata tttttataca catcgcgggc cagtgatgga
     2281 ttatacaaat caatcattag tagcattttt ctttaaagca ttaacgtcat atttaaagaa
     2341 acaaaattgt ttatatgtcc ttgtagatcc atatttaatt gaaaatttac gcaatgcaga
     2401 cggtgaaatt gttaagtctt atgataaccg agcatttgtt agaacaatgg ataaattagg
     2461 ttataaacac caaggtttcc ctgtaggtta tgattcaatg agccaaatcc gttggctgtc
     2521 agtgttagat ttaaaagata agactgaaga ccaactttta aaagaaatgg attatcaaac
     2581 gagacgtaat attaaaaaaa catatgatat tggtgtcaaa actaaaacgt taacgattga
     2641 tgaaacgcaa acctttttcg acttattcca tatggctgag gaaaagcacg gtttcaaatt
     2701 ccgtgagtta ccatactttg aagaaatgca aaaactatat ggtgatcacg ccatgttaaa
     2761 gttagcttat atcgatttaa atgattattt gaaaacatta caattgaaac aacagcaatt
     2821 agctgctgag ctctcaggtg ttgaagaggc attggaagaa agtccgaatt caaagaaaaa
     2881 taaaacgaaa cgaacacagt tagaacaaca gttaagcagt aataagcgaa aaattgataa
     2941 tacaatagaa caaattgaac aagatggtgc agtgttgaac ttagcttctg ctttgtttat
     3001 ctacaatgag catgaagttt attacttatc tagtggttca aaccctaaat ataatgctta
     3061 tatgggtgcc taccatttgc agtgggaaat gattaagttt gctaaagcgc atcatattga
     3121 tcgctataat ttctatggta ttaccggtga cttttcagag tcatcagaag attatggcgt
     3181 acaacaattc aaaaaaggat ttaacgcaca cgtcgaagaa tatattggtg attttattaa
     3241 accaatcaaa cctatgcttt acaaattaca aacatactta aaacataaac gccgttaaca
     3301 gtttatgctg taacatcaaa aaccacgaat cttgctagtg gcatcttcgc cgtaatcaaa
     3361 caagattcgt ggttctattt gtttattctt cattaataac atttaaaaaa cgttttagtt
     3421 cttcagtttt tggattgtta aaaatctctt caggtgcccc ttgttctcca attacacctt
     3481 catgaatgaa tacaatgtta ttagatactt ctctagcaaa acgcatttca tgtgtcacaa
     3541 tgaccattgt catgccctca ttcgctaaat ctttaatcac ctttaacaca tcattcacaa
     3601 gttcaggatc aagtgcagat gttggttcat caaacaacat cactttagga ttcatagcta
     3661 atgctctcgc aatggcaaca cgttgctgtt gaccacctga taatgcatgt ggtcgttgat
     3721 ctttgacatg tgtcaaacca actttctcaa gtaattctag agatttctca cgagcttcag
     3781 cctttttcaa ctttttaact gtaataagac cttccattac attttctaat gccgtcttat
     3841 gcggaaaaag attatagctt tgaaatacca taccagattg tttacgaact tctatttgtg
     3901 attttttatc tttagatgta tatgttttgc cgttaacaaa tactgtacct tcagttggaa
     3961 tttctaacgc attaatcatt cgtaacaacg ttgttttacc tgacccagat cggccaatta
     4021 aggttacaac ctcacctttg tcaacagata aatcaatacc tttgatgact tcaacatcat
     4081 taaatgattt atggatattg ttcaattgaa tcatgagcga taccctcttt caatataaga
     4141 ttcgtagata ccttggatta ttgaaataat aaagcatacc acccagtaca ttagcgcaac
     4201 taacacataa attgttaaat attcatatgt tgtagacgca acttcttgag cttttctaaa
     4261 catttcagcc actaaaataa atcctagtaa tgatgtatct ttgattaaac ttaaaaatgt
     4321 attacctagt gcaggtatag atactcgaat tgcctgcggt aaaatgatgc gctgtatcgt
     4381 ttgacgatat gtcataccga tagaatatgc agcttctgtt tgtccttttg gtatagaaat
     4441 aataccgcca cgaataattt ctgaagcata tgcacctaca tttaatgaca aaccaataat
     4501 ggcagcgacc acaggcgcca atgtccattg attatctgca tcattagtta ataatctacc
     4561 taattctgga ataccataaa agatgataaa taattgtact atcataggtg tcccacgaat
     4621 aatagataca tagacacgtg caatacttct taaaacttta ctcgttgaaa tgcgcatcaa
     4681 tgctgtaaat aatgcaatga ttaaccctaa tacaaatgtt acgagggtaa tcggaattga
     4741 atatttcacc agaccttcga gcataggccc aaaagcttgt tttgcggcat caagtgcatg
     4801 tagttgttca ctatttagat ttagaaacat cttgaccaaa ccatttctta cctattttag
     4861 ctaattcacc gttttcctcg atttttttca agccatcatt gaatttttga actgtttcgt
     4921 catccacttt tttagaaaat gcaaatgcag atttactttg ttcagcgtta cctttgattg
     4981 ccttgatctt agcattaggt ttttgttttt tataatctaa gtatgacaga ctgtcattaa
     5041 atgtaccatc aacacgctta gacaataata aatccattga ttggttaaag ccatcaactt
     5101 ttgtaatatc agcaccttta tccttagcta atttaccata attagatgtg aatgtttgtg
     5161 ctaacttctt acctttaaca tcatcaaaat ctttaatatc tttttcattt tcacgaataa
     5221 ctaaaacagc acttgagaat gtgtaaggtt tagaaaattt atatttcttt tctctatcag
     5281 gattaatacc cacttggttc gcgattacat caaaacgccc tgcgtctaaa cctgcaaaca
     5341 tagaatccca agaagtttca ttaaatttaa gttttaaacc ttcttcttta gctactgctt
     5401 taataacatc aatatcgtaa ccagttaatt taccatcttt atcatggaaa ctaaatggtg
     5461 cataagtccc ctcagtacca acattaattg tcttgctatc tttactagct tccttgtctt
     5521 tagacgaatt gttcccacat gcagctaata tgaaaacgaa agctatcacc acaaataaaa
     5581 gtcttttcat agaatatatc tccttattct tattattcta atcggaataa aaatatttta
     5641 atctgaaatg ccggataagt caatatgttg aacttttgct aaaaagcttt atatagctag
     5701 caacttatat ttattgatgt gaatcaactt cttttttctt tccttgtaac ataaatgaac
     5761 aaaagaaact aatcactgta atcaatagtg caaataaaaa tccagcgtga taaccatgta
     5821 acatcgtatc aactttaatt tgattcatca tctcaatttt gttcatgcca ttatagtctg
     5881 acatagttgg agaaaagttt ttgcttgcat gagataaaat agtaattaat gcagctgttc
     5941 cgatagaacc agcaatttgt tgtactgtgt ttgtcataga tgaaccgtgt gcgttcattt
     6001 caggttttaa ttgattcatt gtatgcgtca taacaggaat taaacctaag gcgataccaa
     6061 ccattcgaat cgcataaacc gttgccaaca taattgtcga tgtctgttca tccattacaa
     6121 caaagtaacc agtggtaata ataacgattg ccataccgac taaagctaat tttcttggac
     6181 cgaacttttc atataaggca cctgaagtca tcgacataaa tgccataaca attgctcctg
     6241 gtaataaagt taacccagaa tctaaagctg aacgatgcaa taaattttgc acaaaaattg
     6301 gtaaaatcgt ttccgaacca atcattgata ccattgtgac acccataata atgataccaa
     6361 ctgtaaattg tttattttta aatactgcaa aatttaataa tggtgttgat agccgatatt
     6421 gacgtaatac aaataatgcg actaaaacaa cgcctccaat catcgaaact aatacgatag
     6481 gattatcaaa acctttttcc gaaattgaac tcgttccata caataacccg ccgaaaccta
     6541 gtacagaaaa aatgacagaa ataaaatcaa taggtacttt aattgtagtt ccaacatttt
     6601 ttatcgtttt aaatccaatt aaaaatgtca cagctgcaat tggagcgaca acatggaaaa
     6661 gcgatctcca gttaaaatat tcaacaaggt aacctgaaag agtaggtcca atagctggtg
     6721 ctaaaccaat aacgaggcca aatatgccca tatatttacc acgctcatgc ggttgaaaaa
     6781 tatctaaaat cgttgtcatc attaaaggca tcataattcc agcacctaac gcctgaataa
     6841 ttctagcaac taataatgtt gcaaagtgtg gactcaaacc gccgagtaat gtacctaaga
     6901 aaaagataga aatacccact aaaaacactt gtcttgttgt ataacgttgt ataacaagcg
     6961 cggacaaagg aataacaatc ccattagtca ataaaaaagc tgttgtcagc cattgaactt
     7021 gtgtatatga aatttcaaaa tctttcataa tacttggtaa agccgtcact aatagtgttt
     7081 catttaataa tccaaaaaat ccacccatca gcatagtaat aatcatcgtt aatttttgtt
     7141 tcttcgacat ttaaagcttc cccctcacca tctaaaaaac gacacacatt tttagatatc
     7201 cggtaattat acccattttc aaaaaaatta gcacgacttt attacaattt ctttaaaacg
     7261 taatcattac tatttacata tgagtagtta tcggtcataa tatatttaca aggagggata
     7321 gagatgaaat tagatttaca gactgctcgt cgtaatttaa atagccctaa cattaaaaca
     7381 agaaagcgtg ctttaaagat tattaagcaa cacaaaagag cgaaataatt gttttaattt
     7441 gactatattg tatgtctaac tatattttcg agtatcccta gctcgttaat ataaactcta
     7501 tacttaacta tttccttaca ttacttcaca tatttaagag ctgcatatac actcactcct
     7561 tgtgtacatg cagctctttt tttataagta gtatttatct ataacttcta aatcatcggt
     7621 taattcataa acaagcggtg cacctgtttt aatttcataa ttaatgatat cttcatctga
     7681 cacatcttca agatatttaa tcaatgctcg aattgaattt ccgtgtgcag aaactaatac
     7741 cgtttgacca tctagcaaat attgtgaaat atgatctgtc caaaatggta tcactcgaac
     7801 taaagtatct ttcagacttt cagaataagg catcatacgt ttatctaaat gattatatcg
     7861 acgatcagct aagtaagctt cacgttgttc ttcggtttca gcaggcggtt tcacatcata
     7921 agaacgacgc caaatatgta cttgttcttc tccaaattct tttctagcat catctttatt
     7981 taagccttgc aatccaccat agtggcgttc atttaaacgc cagcttttat atacaggaat
     8041 ccattgttgt ttagattcag ttaaaatata atgcgttgta tctaaagcac gtgttaataa
     8101 cgatgtaaaa gctacatcga tggcaatgtt attttctctt actttttcac ctgctctagt
     8161 cgcttcatta ataccttgtt cagataaatt aacatcttcc catccagtaa ataagttttt
     8221 agcattccac tcgctttgtc catgacgaca taaaattaat tttggcataa cttataacct
     8281 ctttccttta atctttagga cgttatttca gggatatttt aagatgattt aatcttagca
     8341 attttctttt caatatgttt taaaattcaa tgattaaatc aattatcact aaacattaaa
     8401 tacataaatc ctattcccca ttttcatttt ttaattcata aatgaatcaa taaccaccta
     8461 ataacaaatc atattataca cctttgttcg ccatttttct aaggtttaaa aaatattttt
     8521 aggtgcacct aaaaataaat gtaataaaaa cgcctcctca gatatttata tatctatgaa
     8581 gacgtttaaa tacattatag atggtctggt tctgggtgaa cgtatactga ggaaatacct
     8641 tttttgtgca aatgatgttc gacattgtca caaatttgat gcgctgctac taaggaaagg
     8701 ttagcatcta caacaattgt gacatcaata aacacactac ttccatggta acgcccttta
     8761 atacttttaa cttcttgtac ttcatcaact tctaaaatat cattgcgata cgcttctaat
     8821 tcagtttcat tgaaaccatc actcaacata aaaattgctt ctttaaaaat accaaaacca
     8881 gtataaacga ttagtaagcc tagcaatgtt gctaaaataa tatcgacaat tgggaaaccg
     8941 atttgcgtaa aaattaatcc tatcgccgtt ccaatgctga ctaaactatc tgataaatta
     9001 tctttggcgg cagaatttaa agaactactt ttcgttcttt tcgctagtct ttgattgact
     9061 gcaaatacaa tcaacattac aagaccactg attaagctga cgataattgt tattgcgtta
     9121 ggtacaacgt catcttcttt gaacaaacga ggtgcatttt gaataactac ttggatacct
     9181 acaaacataa tgacaaatga caccaataat gaagaaatat tttcagactt caaatggcca
     9241 taaggatgat ttcgatcggc aggtttaatt gaaattttca atccaataat aacagctaaa
     9301 gaaacgataa tatctgtcat attgtttaat gcgtcggctc ttacagctgc agagttaaag
     9361 acaaaacctg tgacatactt aacaatagat aagattatat atacaatcaa actcaaataa
     9421 gcaccgcgtt gcgccaattt aagattttca ttatgagaca tgcgttgaac caccttgaat
     9481 tagtatagta acaatattat gaatgattta ttttaatttt acaacgtttt taatttttat
     9541 aaatttttat aaaataaaac taatttattc attcgcaacc cctaaaaata ttttttagcc
     9601 tttctgcgaa ttttatgagc tagaaaggct tatgcattag tagcgaagct atctaatgcg
     9661 ttcctttatt ttctgcgaat tttatgagct agaaaggctt atgcagttga cgttttacgt
     9721 ccaactgcgt tccttcgtct tcttcaaatt tatttgttag aaaggcgccc aactctccct
     9781 gtttgtgaac tttcgcctcg aaagttgcta tgttgggacc ctatgcatta gttgcgaagc
     9841 tatctaatgt cgtgacctaa ttatatagaa gaaaaagtgc atcagtgaca aatcaaatga
     9901 gatttctact ctaccaaact ctcttcgaaa cacaattttc tcctctattt attagcaaac
     9961 tattgcattt ctccatatag tacttcctta cttaaaatac gctgaatgtt ctgaattaaa
    10021 cgctttttta catagttaac actagttaat ctattagtta acattagtta ataattagtt
    10081 aatttccatt tgtattctca tgtgataaat tctaaaagca tacaataaat ttaatatgta
    10141 aaaagaaagg gaatacacat gaaaaataaa tatatctcga agttgctagt tggggcagca
    10201 acaattactt tagctacaat gatttcaaat ggggaagcaa aagcgagtga aaacacgcaa
    10261 caaacttcaa ctaagcacca aacaactcaa aacaactacg taacagatca acaaaaagct
    10321 ttttatcaag tattacacct aaaaggtatc acagaagaac aacgtaacca atacatcaaa
    10381 acattacgcg aacacccaga acgtgcacaa gaagtattct ctgaatcact taaagacagc
    10441 aagaacccag accgacgtgt tgcacaacaa aacgcttttt acaatgttct taaaaatgat
    10501 aacttaactg aacaagaaaa aaataattac attgcacaaa ttaaagaaaa ccctgataga
    10561 agccaacaag tttgggtaga atcagtacaa tcttctaaag ctaaagaacg tcaaaatatt
    10621 gaaaatgcgg ataaagcaat taaagatttc caagataaca aagcaccaca cgataaatca
    10681 gcagcatatg aagctaactc aaaattacct aaagatttac gtgataaaaa taaccgcttt
    10741 gtagaaaaag tttcaattga aaaagcgatc gttcgtcatg atgagcgtgt gaaatcagca
    10801 aacgatgcca ttactaaatt acaaaaaaat gattcaattg aaaacagacg tttagcacaa
    10861 cgtgaagtta ataaagcacc tatggatgta caaaagcact tacagaaaca attagacgca
    10921 ttagtagctc aaaaagatgc tgaaaagaaa gtggcgccaa aagttgaggc tcctcaaatt
    10981 caatcaccac aaattgaaaa acctaaagca gaatcaccaa aagttgaagt ccctcaaatt
    11041 caatcgccaa aagttgaggt tcctcaatct aaattattag gttactacca atcattaaaa
    11101 gattcattta actatggtta caagtattta acagatactt ataaaagcta taaagaaaaa
    11161 tatgatacag caaagtacta cactgataaa tattttaaat ataaaggtac aatagataaa
    11221 actgtactat cagtttttgg taatggatac aaatcataca tccaaccatt aaaagttgaa
    11281 gatcaaaaaa attatgtatc taaatcatat gcacaagtaa gaaactatgt aactgaaact
    11341 ttaaacacag gtaaagtatt atatgcattt tatcaaaatc caaaagtagt taaagctgct
    11401 ataactacag ctgaaacagc aacatcaatc aaaaatatct tctctagttt tacatcattt
    11461 tttaaataat tttcgtaata aaccgtgatt aaaaagaacc gttgattctc aatcgaatct
    11521 acggttcttt tttcattttc catcaattaa atgtttcttc gctacttgtc agcccacttt
    11581 tttacctgca acttgttaaa taatccttac atcgttaacg aatagttcat catttagttg
    11641 aatcagctca actttattaa cttcaaattt tcacaaacta ttgcgcaatt cattcccctt
    11701 ccacttcaag caccttaatt aaacattaat tcaatagaat aagacttgca aaacatagtt
    11761 atgtcgctat ataaacgcct gcgaccaata aatcttctaa acataacata atgcaaaaac
    11821 atcatttaac aatgctaaaa atgtctcttc aatacatgtt gatagtaatt aacttttaac
    11881 gaacagttaa ttcgaaaacg cttacaaata gattattata tatatgaact taaaattaaa
    11941 tagaaagaaa gtgatttcta tgattaaaaa taaaatatta acagcaactt tagcagttgg
    12001 tttaatcgcc cctttagcta atccatttat agaaatttct aaagcagaaa ataagataga
    12061 agatatcggg caaggtgcag aaatcatcaa aagaacacaa gacattacta gcaaacgatt
    12121 agctataact caaaacattc aatttgattt tgtaaaagat aaaaaatata acaaagatgc
    12181 cctagttgtt aagatgcaag gcttcatcag ctctagaaca acatattcag acttaaaaaa
    12241 atatccatat attaaaagaa tgatatggcc atttcaatat aatatcagtt tgaaaacgaa
    12301 agactctaat gttgatttaa tcaattatct tcctaaaaat aaaattgatt cagcagatgt
    12361 tagtcaaaaa ttaggctata atatcggcgg aaacttccaa tcagcgccat caatcggagg
    12421 cagtggctca ttcaactact ctaaaacaat tagttataat caaaaaaact atgttactga
    12481 agtagaaagt cagaactcta aaggtgttaa atggggagtg aaagcaaatt cattcgttac
    12541 accgaatggt caagtatctg catatgatca atacttattt gcccaagacc caactggtcc
    12601 agcagcaaga gactatttcg tcccagataa tcaactacct cctttaattc aaagtggctt
    12661 taatccatca tttattacaa cattgtcaca cgaaaaaggt aaaggtgata aaagcgagtt
    12721 tgaaatcact tacggcagaa acatggatgc tacatatgct tacgtgacaa gacatcgttt
    12781 agccgttgat agaaaacatg atgcttttaa aaaccgaaac gttacagtta aatatgaagt
    12841 gaactggaaa acacatgaag taaaaattaa aagcatcaca cctaagtaaa cagttcaatc
    12901 attttaaaaa atcctgggac acttcatact tgtctcagga ttttttaaca tattgaatca
    12961 gcctcataac attaaattat tttatcgtac attaaattta ataataacaa ctgattttta
    13021 taagaacaaa gtatcgcacc atagtagata cacaaataat acaaatgaag caatttaagt
    13081 tgaaagctta aattaatatt ctcaagttaa taaacaatta acttttagat ggattcatca
    13141 aaaatcgtaa aaaagcacaa tttgtatttt acaaacatta attaaaaatg aaagcaaaac
    13201 attcgtgcaa tcggttacct taaattgttt acaactgtca acaataccaa ggttttatta
    13261 actatatttc tcacaaaatt agcttttagc attccaaaca aaaaaggtta aatcgaacgg
    13321 aattatgaca tttttaaagc cattgtaaaa aaagttgata atggtcaatt gttaatgaac
    13381 agttaattat aataacgttc aaaatatatt attatttaat taagttaaat aaaattatag
    13441 aaagaaagtg aaacttatgc ttaaaaataa aatattagct acaactatat ctgtaagctt
    13501 acttgcccct cttgccaatc cgttattaga aaatgctaaa gctgccaatg atactgaaga
    13561 catcggtaaa ggaaacgatg tagaaattat caaaaggaca gaagataaaa caagtaataa
    13621 atggggagtg actcaaaata tccaattcga ctttgtgaag gataaaaaat ataacaaaga
    13681 tgctttgatc ctaaagatgc aaggattcat tagctctcga acaacatatt ataactataa
    13741 aaatacaaat catattaaat ctatgcgttg gcccttccaa tataatattg ggttaaaaac
    13801 aaatgatcca aatgtttctt taatcaatta tttaccgaaa aataaaattg aatctacaaa
    13861 tgtgagccag acattaggat acaatatcgg cggtaatttc caatcagccc catcactcgg
    13921 tggtaatgga tcctttaact actctaaatc gattagctat acacaacaaa attatgtcag
    13981 tgaagtggaa caacaaaact caaaaagtgt tttatggggt gtcaaagcga attcattcgt
    14041 cactgcatca ggtcaaaagt cagcatttga tagtgattta ttcgtaggat acaaaccaaa
    14101 tagcaaagat ccaagagatt attttgttcc agacagcgaa ttaccacctc ttgtgcaaag
    14161 tggatttaac ccttcattta tcgcaacagt atctcatgaa aaaggttcag gcgacacgag
    14221 cgaatttgaa atcacttatg gaagaaatat ggatgtcact catgccatta aaagatcaac
    14281 acattatggc aacagttatt tagatggtca cagagtccat aatgcattta aaaatagaaa
    14341 ctacactgtg aaatatgaag tcaattggaa gacacacgaa atcaaagtga aaggacagaa
    14401 ttgatatgaa catgaataaa ttagtcaaat catccgttgc tacatctatg gcattattat
    14461 tactttctgg tactgctaat gctgaaggca aaatcacacc agttagcgtc aaaaaagtag
    14521 atgataaagt tactttatac aaaactacag ctacagcaga ttcagataaa ttcaaaattt
    14581 cacagatttt aacatttaat ttcatcaaag ataaaagtta tgataaagat actttagtac
    14641 ttaaagctgc aggtaatatt aactcaggtt atgaaagacc aaaccctaaa gactatgact
    14701 tttcaaaaat atattggggt gcaaaataca atgtgtctat cagttcacaa tctaatgatt
    14761 cggttaatgt agtagactat gcacctaaaa atcaaaatga agaatttcaa gtgcaaaaca
    14821 ctttaggata cacatttggt ggtgacatca gtatctctaa tggtttatct ggtggactta
    14881 acggaaacac tgctttttct gaaacaatta actacaaaca agaaagttac agaacaactt
    14941 taagtcgcaa cacaaattat aaaaatgtcg gttggggtgt agaagcacat aaaattatga
    15001 ataatggttg gggcccctat ggccgagata gcttccatcc aacatatggc aatgaaatct
    15061 tcttagctgg tagacaaagc agttcatatg caggccaaaa cttcatagcg caacaccaaa
    15121 tgccattatt atctagaagt aacttcaatc cagaattttt aagcgtacta tcacacagac
    15181 aagatggcgc taaaaaatct aaaattacag taacttatca acgtgaaatg gatttatacc
    15241 aaattcgttg gaatgggttc tactgggcag gcgcaaatta taaaaacttt aaaactagaa
    15301 catttaaatc aacatatgaa attgattggg aaaatcacaa agtgaaattg ttaggtacaa
    15361 aagaaactga aaacaataaa tagctagtaa aacacggtcg ccaacaataa ttgtgacgac
    15421 cgtgttttga tttattatct tagtaagact gccattcttt ttctcaatgt gagatataaa
    15481 ggaatagcta caattaaagt gaatattacg cctggaatcg cgtttaataa cactacccac
    15541 acaggtaaat ttaaaataat agataatagt agcctagata ccaaactgcc taatacactt
    15601 gctaaaacta atgatagtac atttattttc aataaataaa caactgcaat agctataact
    15661 ctaaatataa tagagataat cacattaatt ggattaaata caccaaatac tagtaataat
    15721 aggctagata ataatccacc taaaaagtac tttttaattc caaagaaagc taatatcaat
    15781 aatgctgccg gtgcagataa ttgaaaatct aaacctggta taatggatgg tattttcaaa
    15841 actgccaaaa tggttaaaat cgcagcaatg acactaattt gagtaatatc ttttgatgtc
    15901 atactaaaac ccctataccg tttcataaac aacttgcttc ggtgtgcttt ctaaaaatga
    15961 tatgtaatga tttaaatcaa tacgatcgtc cacaaatatt attctgcctc catatctcgt
    16021 attaactggt ttaatatcaa ataatcgatg gtaaccaatt ttagcagagg caaaataacc
    16081 tgtcgtatac gttaagtcat cggacacaca aagttctcct ttgacatatg gatgcgcatt
    16141 gatacaacta gcaattgcta aggcatcagt cactctttca ttaagatcac ctttattatt
    16201 tatatcttca aacgaaaaat gcgttgccct aatccctctt tgtccaaatg aatctaaacg
    16261 tttaccagat atagcagata gaataatagc tcctgtataa accgtttcat ttttaatata
    16321 tgtcatccct tgatttagcg cttgttcagt gacaccacat tcttgtgtta aatgttggag
    16381 atttgcttta tcatcctcag taatttgtaa tgcttttatt tgttgaatcg gttccatgat
    16441 tttttgtatt tttatattga gaaaatccgg ttgcccattt tcatgaaaaa aacctttatt
    16501 ataaaacctt cgtaccgtct gttctatttt gtcaaactca caaatcgttt cagcaccact
    16561 aatatgaaca tcttgattgc tagaacgcat ttttatacta tacatgacga tcacctcaat
    16621 cttcttgatg caaaatttca aacaacctat ctacatcttg ttcagtatga aaatacgaca
    16681 atgatattct taacattggc ttagtcacag ttggatacct taaataactt gtaaacacat
    16741 gatgctttaa taatgtttga tgaatgttct tagccgcttc tatgtcatca aactcaataa
    16801 acttaatcgg cgagtttgaa ctattataat taacattgag tgtttttaac ttttggttaa
    16861 aatatttaat caaactattt aatttagtgc gtctatcatc agcatttatt aacttttcaa
    16921 tatttctttt tataaaatac aaattataaa ttggcaaact acttgagtag atgagtggtc
    16981 taccgtgatt aattaacata tccttcacat catttgaact taaaatcaca cccccgtatg
    17041 caccacatgc tttagataaa ctagaagtga gtatatctat gccttgataa ttcgagtaat
    17101 tctctattcc aaaactatgt gaaacatcga gtatcagtgt tgcgttatat ttatgcttta
    17161 atgagactaa ttgaccaatg tccacaacgt cgccattcgt tgaaaataca ctatcagata
    17221 tgattatttt ttgtatattt tgattagggt attccgctaa ccttttttct aaatcagcaa
    17281 tatctagatg cttatatatc actttttcta aaccacttaa cttaataccg tcaataatac
    17341 tcgcatgatt ttcttgatct gaaaacacga cacaatttgt atttttgaaa atattaaata
    17401 acgccaaatt agcatcataa ccactattta agatagtaca tgcattatat ccgagccaac
    17461 ctgctaacat tgtttcaatc tcttcataag ctgtcgaact tccactaatt aatcttgaac
    17521 ttgataagtg ataactatac ttccgcataa atctttcgaa atcatcctta tcaaacgcta
    17581 tttgtcctaa tcctaaataa tcattagatg tatagttcgt acatcgctta ttttctactt
    17641 caatatactg tctatctata taccctaccg atttaagcga ccgatataac cctttctgtt
    17701 gtaataaatc aatttgctct tgaaacatca ttcttgtttt ccttattttc acaagtgtca
    17761 taatcaattt caaagcccaa atcattaatc atatcgtagt ctaattggtt cggttgccca
    17821 ccagtaatta gataatcacc gacaaatatt gaattcgccg cttttaatgc taatggctgt
    17881 aacgaacgta agttgacctc tcttcctcca gcaatacgaa tttctttcgt aggattgatt
    17941 aatcggaata atgctacgat tcttaaacat ttcattggtg ttaaatcatc catgctaccg
    18001 aactttgtac ctttaattgg atgtaaaaaa ttaatcggaa tactgtcggc atccatttct
    18061 ttcaaagcaa atgccatatc aacaatatct tgattagatt ctcccatacc acaaatcacg
    18121 ccagaacatg gtgatatatt attcgctttc attagttcta tcgtatctgt tctatcttta
    18181 taactatgcg ttgtcacgac gttatcatgg taattttcac ttgtattaat attgtggtta
    18241 tatctgtcta caccagcaga cttaagtttc ttagcttgtt cgtcattcgt taatcctaaa
    18301 catgcacata tttttagttg cgggtgttga gatttaatcg ttcttacagt gttactaata
    18361 tgatcaactt ctttatcgct cggccctcta ccactcataa caatacaata tgttccaata
    18421 tgattatcat gtgctacctt tgctccatcg ataatttgtt cctctggaat taaagcatat
    18481 cgctgttttt gtttaatatc tcgtgattgt ccacagtacc cacaattctc aggacatata
    18541 ccacttttag catttaaaat catatttaat tttacttttt taccgaaata atgttttctt
    18601 aaaatgtacg cctcatttaa taaatctaag gtatcaatat tagtatcctc ataaattttc
    18661 aatacagtct cttttgttaa ttgttcccct tgtaatatgc gtttagccaa attcatatta
    18721 acacttccta tctaaaatgt tatgtactgc ctttttaaaa atacgtacca tcgttttcac
    18781 ttctttattt gacatactta acactggcac aaacgtaatg acattttcta aatttctaat
    18841 cattagccca ttttctttac aattacgtac aatacgttca actttttcaa tttctaacgg
    18901 cgttttacta tctttatctg taaccaattc aactccaaac attaagcctc gtcctcttac
    18961 atcaccaaca tttcgatgac gcttcagcgc atgtaactgt ttctcaagtg ttgatgatgt
    19021 cgtttcaata tgtgacaata acttacgttt ttcataaagt cttatatttt ctaatgcaac
    19081 cgtacaaacg atttgatttc cggtgtatgt atgaccatgg aaaaaggtat tcacaccatg
    19141 cgaatcactt aaaaatgcat tgtatatttt ttgagatgtc aatgtaactg caagtggtaa
    19201 gtagccacct gtaatcgcct tacctaaaca cataatatcc ggttgaacat cttcatgatt
    19261 acatgcaaac atctttccag ttctcccaaa accaacagct acctcatcac aaattaataa
    19321 gacatcgtat tttttgcaca atttctcgac ttctttcaaa aagcctttag gatgaacaaa
    19381 caagcctgtc gcaccttgaa tcaacggttc caatataaac cctgcgattt catcatttct
    19441 ttcaattaga atttgctcta tattcgttaa aatagctgtc atcatttcat tttcagtatc
    19501 gtaattactg cgatagaaag atgggctttc tacttgaata ttctcaaata ttaagtcttt
    19561 aaatatttta tgaaaggtct tgataccacc aacacttacc gcaccaatcg tatccccatg
    19621 ataaccgtaa tttagcgtta taaacttgtt tttcttggca tatttttctc tatcaatatt
    19681 tttccaatac tgatatgcca tctttattgc gatttcaaca gacgcactgc ctgtatcaga
    19741 ataaaatact tttcttagat tacttggcgt gatttcgatt aatttttccg caagttctat
    19801 tgacggaata tttgatgatc ctagcagcgt agaatgggca attttattga gttgcttttt
    19861 aattacctta tttaagtatt tattattatg accatgcaca ttgacccaca acgatgcata
    19921 accatctaaa tatttattgc cattcgtatc gtaaaggtag ctaccctttc ctttttcaat
    19981 gatgattgct tcttctttgc tatatacacc catttgtgta aatggatgcc aaacatattc
    20041 tgagtctttt tgtttaagtt gttgtgtgta attcattttt aactcctatt aattgtttta
    20101 aaaatgcttc tgagaaattt tcatacgtgg catgttcttc aaatgtatag actgttttat
    20161 ttgttaattt tccaatcgtc atttgattgt ctttttcaat atagctgtct gtatagcgat
    20221 tcattattaa aaaattactc gctgatacat tctgattaac ataatcttgg tgaacaatgg
    20281 catcgctaat agcacctaat tttgatggca acacactgat gacacaatct gcacaatcat
    20341 tgattagatc tttagtcatg tagaaatcat ctgtaccttc atatattggt acggcaattc
    20401 ccccagcccc ctcaattaag ataaaatcaa attccttatc taaaactttt accttatcta
    20461 atacacgctg cttatttaga aaaatttgat ctgccatttt aaatgcaagg tgtggtgata
    20521 caggttgctt aaaagtataa agtgacgtta tgtcatagct taaatcacat tcatttttaa
    20581 atacttctaa atctggaaat gtcccgtctt ggcgttcctc agtttgaaat ggtttaaaaa
    20641 cacaaacacg atgaccacgt gttttcaaag catggtataa atgctttgta acataggttt
    20701 tgcctacatc agtattcgta cttgtaataa aaatcctcat aaaaaataag cacctcaatc
    20761 cgaatgtaaa cttttataat taataagttt acatttaagg cgcatgtttt tcaagaaaac
    20821 atgaatttaa acttctcagt ataactataa attttggcaa aattttgacc ttgctattga
    20881 aatgactaag gcttcttaca gaattcgggc agatataaac agcgattcga taaaatatgt
    20941 cagttaaaat taataattgc attatttctt aataaaaaga catcctagct ttaccatgtt
    21001 gtgaaacaat ccattaattg taatattacg ttgagcgcct tgttcatcaa tgatataatt
    21061 agttacataa taaattgaac atctaaatac accaaatccc ctcactattg ccctagtgag
    21121 gggatttata ctccggtaaa taaccgaagg tattttgaat cattgttaaa aatggaatta
    21181 aagttctagt atctgtcggg ttttgaaata ggtcatagga taaaacaaat tgagaatttg
    21241 tcgctatttg taaattgtat cctggcttaa gttggccgtt tttcatatgg tcttccttca
    21301 ttctcataaa agttgcatca tgatcagttt tagaaaagct atttctatct ttaagaatcg
    21361 atttttgttc ttcatattta ctttttcttt cggaataatc atcaaatttc tttttgaact
    21421 tcttaatctc agttcttttt ttacgggtct gttttctaat ttgagcacaa tcttcgtt
//
LOCUS       DAHHOJ010000040        21293 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.040, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000040 DAHHOJ010000000
VERSION     DAHHOJ010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 21293)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21293)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21293
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.040"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            231..1169
                     /locus_tag="NBH32_001273"
     CDS             231..1169
                     /locus_tag="NBH32_001273"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HCD1514887.1"
                     /translation="MANIVFKLSDKDITTLMSRISFDTENLPQGMKARAKYQNTTVNI
                     YQSGKVMFQGNHAEAVSQELLPQHSQLNTNKTKKKNMANSFLEQSLMYDQFNCIGSDE
                     AGSGDYFGPLTVCAAFVTKEHVLILKTLGVDDSKKLTDTKIVELAEQLVTFIPHSLLT
                     LHNEKYNIQQAKGWTQVKMKAVLHNEAIKNVLEKIDSSQLDYIVIDQFAKREVYSHYA
                     LSDIPLPKKTKFETKGESKSLAIAVASIISRYAFVTYMDQISKNINMTIPKGAGAKVD
                     VIAAKIIKKYGLSRLDTISKKHFKNREKAQKILKPL"
     gene            complement(1472..3874)
                     /locus_tag="NBH32_001274"
     CDS             complement(1472..3874)
                     /locus_tag="NBH32_001274"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCD1514888.1"
                     /translation="MLISNEWLKEYVTIDDSVSNLAERITRTGIEVDDLIDYTKDIKN
                     LVVGFVKSKEKHPDADKLNVCQVDIGEDEPVQIVCGAPNVDAGQYVIVAKVGGRLPGG
                     IKIKRAKLRGERSEGMICSLQEIGISSNYVPKSFESGIYVFSESQVPGTDALQALYLD
                     DQVMEFDLTPNRADALSMIGTAYEVAALYNTKMTKPDTTSNELELSANDELTVTIENE
                     DKVPYYSARVVHDVTIEPSPIWMQARLIKAGIRPINNVVDISNYVLLEYGQPLHMFDQ
                     DAIGSQQIVVRQANEGEKMTTLDDTERELLTSDIVITNGQTPIALAGVMGGDFSEVKE
                     HTSNIVIEGAIFDPVSIRHTSRRLNLRSESSSRFEKGIATEFVDEAVDRACYLLQTYA
                     NGKVLKDRVSSGELGAFITPIDITADKINRTIGFDLSQNDIVTIFNQLGFDTEINDDV
                     ITVQVPSRRKDITIKEDLIEEVARIYGYDDIPSTLPVFEKVTSGQLTDRQYKTRMVKE
                     VLEGAGLDQAITYSLVSKEDATAFAMQQRQTIDLLMPMSEAHASLRQSLLPHLIEAAS
                     YNVARKNKDVKLFEIGNVFFANGEGELPDQVEYLSGILTGDYVVNQWQGKKETVDFYL
                     AKGVVDRVSEKLNLEFSYRRADIDGLHPGRTAEILLENKVVGFIGELHPTLAADNDLK
                     RTYVFELNFDALMAVSVGYINYQPIPRFPGMSRDIALEVDQNIPAADLLSTIHAHGGN
                     ILKDTLVFDVYQGEHLEKGKKSIAIRLNYLDTEETLTDERVSKVQAEIEAALIEQGAV
                     IR"
     gene            complement(3874..4932)
                     /gene="pheS"
                     /locus_tag="NBH32_001275"
     CDS             complement(3874..4932)
                     /gene="pheS"
                     /locus_tag="NBH32_001275"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCD1514889.1"
                     /translation="MSEQQTMSELKQQALVDINEANDERALQEVKVKYLGKKGSVSGL
                     MKLMKDLPNEEKPAFGQKVNELRQTIQNELDERQQMLVKEKLNKQLAEETIDVSLPGR
                     HIEIGSKHPLTRTIEEIEDLFLGLGYEIVNGYEVEQDHYNFEMLNLPKSHPARDMQDS
                     FYITDEILLRTHTSPVQARTMESRHGQGPVKIICPGKVYRRDSDDATHSHQFTQIEGL
                     VVDKNVKMSDLKGTLELLAKKLFGADREIRLRPSYFPFTEPSVEVDVSCFKCKGKGCN
                     VCKHTGWIEILGAGMVHPNVLEMAGFDSSEYSGFAFGMGPDRIAMLKYGIEDIRHFYT
                     NDVRFLDQFKAVEDRGDM"
     gene            complement(5313..6053)
                     /locus_tag="NBH32_001276"
     CDS             complement(5313..6053)
                     /locus_tag="NBH32_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HCD1514890.1"
                     /translation="MEQITSAQNNRIKQANKLKKKRERDKTGLALIEGVHLIEEAYQS
                     GIVITQLFAIEPARLDQQIIAYAQEVFEINMKVAESLSGTVTPQGFFAIIEKPQYDIS
                     KAQQVLLIDRVQDPGNLGTLIRTADAAGMDAVIMEKGTTDPYQDKVLRASQGSVFHLP
                     VMTQDLDTFITQFNGPVYGTALENAVAYKEVTSSDSFALLLGNEGEGVNPELLAHTTQ
                     NLIIPIYGKAESLNVAIAGSILLYHLKG"
     gene            complement(6293..6400)
                     /locus_tag="NBH32_001277"
     CDS             complement(6293..6400)
                     /locus_tag="NBH32_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514891.1"
                     /translation="MVLLAFVTTIHDTDDGRFFNEHKHANNKLLKRSLI"
     gene            complement(6437..6760)
                     /gene="isdG"
                     /locus_tag="NBH32_001278"
     CDS             complement(6437..6760)
                     /gene="isdG"
                     /locus_tag="NBH32_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylobilin-forming heme oxygenase IsdG"
                     /protein_id="HCD1514892.1"
                     /translation="MKFMAENRLTLTKGTAKDIIERFYTRHGIETLEGFDGMFVTQTL
                     EQEDFDEVKILTVWKSKQAFTDWLKSDVFKAAHKHVRSKNEDESSPIINNKVITYDIG
                     YSYMK"
     gene            complement(6779..7513)
                     /gene="srtB"
                     /locus_tag="NBH32_001279"
     CDS             complement(6779..7513)
                     /gene="srtB"
                     /locus_tag="NBH32_001279"
                     /EC_number="3.4.22.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class B sortase"
                     /protein_id="HCD1514893.1"
                     /translation="MRMKRFLTIVQILLVVIIIIFGYKIVQTYIEDKQERANYEKLQQ
                     KFQMLMSKHQAHVRPQFESLEKINKDIVGWIKLSGTSLNYPVLQGKTNHDYLNLDFER
                     EHRRKGSIFMDFRNELKNLNHNTILYGHHVGDNTMFDVLEDYLKQSFYEKHKIIEFDN
                     KYGKYQLQVFSAYKTTTKDNYIRTDFENDQDYQQFLDETKRKSVINSDVNVTVKDKII
                     TLSTCEDAYSETTKRIVVVAKIIKVS"
     gene            complement(7575..8540)
                     /gene="isdF"
                     /locus_tag="NBH32_001280"
     CDS             complement(7575..8540)
                     /gene="isdF"
                     /locus_tag="NBH32_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000594537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin ABC transporter permease protein IsdF"
                     /protein_id="HCD1514894.1"
                     /translation="MIKNKKKLLFLCLLVILIATAYISFVTGTIKLSFNDLFTKFTTG
                     SNEAVDSIIDLRLPRILIALMVGAMLAVSGALLQAALQNPLAEANIIGVSSGALIMRA
                     LCMLFIPQLYFYLPLLSFIGGLIPFLIIILLHSKFRFNAVSMILVGVALFVLLNGVLE
                     ILTQNPLMKIPQGLTMKIWSDVYILAVSALLGLILTLLLSTKLNLLNLDDVQARSIGF
                     NIDRYRWLTGLLAVFLASATVAIVGQLAFLGIIVPHVVRKLVGGNYRVLIPFSTVIGA
                     WLLLVADLLGRVIQPPLEIPANAILMIVGGPMLIYLICQSQRNRI"
     gene            complement(8553..9431)
                     /gene="isdE"
                     /locus_tag="NBH32_001281"
     CDS             complement(8553..9431)
                     /gene="isdE"
                     /locus_tag="NBH32_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006190048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter substrate-binding protein
                     IsdE"
                     /protein_id="HCD1514895.1"
                     /translation="MRIIKYLTILVISVVILTSCQSSSSQESTKSGEFRIVPTTVALT
                     MTLDKLDLPIVGKPTSYKTLPNRYKDVPEIGQPMEPNVEAVKKLKPTHVLSVSTIKDE
                     MQPFYKQLNMKGYFYDFDSLKGMQKSITQLGDQFDRKAQAKELNDHLNSVKQKIENKA
                     AKQKKHPKVLILMGVPGSYLVATDKSYIGDLVKIAGGENVIKVKDRQYISSNTENLLN
                     INPDIILRLPHGMPEEVKKMFQKEFKQNDIWKHFKAVKNNHVYDLEEVPFGITANVDA
                     DKAMTQLYDLFYKDKK"
     gene            complement(9428..10504)
                     /gene="isdD"
                     /locus_tag="NBH32_001282"
     CDS             complement(9428..10504)
                     /gene="isdD"
                     /locus_tag="NBH32_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-regulated surface determinant protein IsdD"
                     /protein_id="HCD1514896.1"
                     /translation="MRNVKQIATKSIIAIISLGILTYTTMIGSVLADEIKYPSAKFNQ
                     PEAKDKTELTTSIFDEKIKENKALELVIFNQENKNVTEEQQLVDEKAQLISDMTGKIY
                     LQVKLKGQIDKEQLVFQNDKNEEFPFVIKDEKDDTIVRILIEQHMDKINMHVKTLAEK
                     KNLDNKEMVYSIHFKEKKVQHDDAKEVPSKHQSQENNQDQLKKDIDDKKDSQKSDIKE
                     RRTSLFTEKGLNDIPVQKDKVQQDSNKKIENERPKASGTLKVENNPSTVKKVENNHKE
                     QPKHKDEKSKKEKKKVVEKEKALPAFNRDDDSKNSSQLSSDIKELDEPNHKKQYMLFA
                     AGIVLATILLISAHLYSRKRGNQV"
     gene            complement(10504..11187)
                     /gene="isdC"
                     /locus_tag="NBH32_001283"
     CDS             complement(10504..11187)
                     /gene="isdC"
                     /locus_tag="NBH32_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdC"
                     /protein_id="HCD1514897.1"
                     /translation="MKNILKVFNTTILALIIIIATFSNSANAADSGTLNYEVYKYNTN
                     DTSIANDYFNKPAKYIKKNGKLYVQITVNHSHWITGMSIEGHKENIISKNTAKDERTS
                     EFEVSKLNGKIDGKIDVYIDEKVNGKPFKYDHHYNITYKFNGPTDVAGANAPGKDDKN
                     SASGSDKGSDGTTTGQSESNSSNKDKVENPQTNAGTPAYIYAIPVASLALLIAITLFV
                     RKKSKGNVE"
     gene            11396..12448
                     /gene="isdA"
                     /locus_tag="NBH32_001284"
     CDS             11396..12448
                     /gene="isdA"
                     /locus_tag="NBH32_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored heme-scavenging protein IsdA"
                     /protein_id="HCD1514898.1"
                     /translation="MTKHYLNSKYQSEQRSSAMKKITMGTASIILGSLVYIGADSQQV
                     NAATEATNATNNQSTQVSQATSQPINFQVQKDGSSEKSHMDDYMQHPGKVIKQNNKYY
                     FQTVLNNASFWKEYKFYNANNQELATTVVNDNKKADTRTINVAVEPGYKSLTTKVHIV
                     VPQINYNHRYTTHLEFEKAIPTLADAAKPNNVKPVQPKPAQPKTPTEQTKPVQPKVEK
                     VKPTVTTTSKVEDNHSTKVVSTDTTKDQSQTQSARTVKTTQTAQEQNKVQTPVKDVAT
                     AKSESNSQAVSDNKSQQTNKVTKQNETPKQASKAKELPKTGLTSVDNFISTVAFATLA
                     LLGSLSLLLFKRKESK"
     gene            12651..14585
                     /gene="isdB"
                     /locus_tag="NBH32_001285"
     CDS             12651..14585
                     /gene="isdB"
                     /locus_tag="NBH32_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme uptake protein IsdB"
                     /protein_id="HCD1514899.1"
                     /translation="MNKQQKEFKSFYSIRKSSLGVASVAISTLLLLMSNGEAQAAEET
                     GGTNTEAQPKTEALASPTTTTEKAPETKPVANAVSVSNKEVEAPTSETKEAKEVKEVK
                     APKETKAVKPAAKADNNTYPILNQELREAIKNPAIKDKDHSAPNSRPIDFEMKKENGE
                     QQFYHYASSVKPARVIFTDSKPEIELGLQSGQFWRKFEVYEGDKKLPIKLVSYDTVKD
                     YAYIRFSVSNGTKAVKIVSSTHFNNKEEKYDYTLMEFAQPIYNSADKFKTEEDYKAEK
                     LLAPYKKAKTLERQVYELNKIQDKLPEKLKAEYKKKLEDTKKALDEQVKSAITEFQNV
                     QPTNEKMTDLQDTKYVVYESVENNESMMDTFVKHPIKTGMLNGKKYMVMETTNDDYWK
                     DFMVEGQRVRTISKDAINNTRTIIFPYVEGKTLYDAIVKVHVKTIDYDGQYHVRIVDK
                     EAFTKANTDKSNKKEQQDNSAKKEATPATPSKPTPSPVEKESQKQDSQKDDNKQLPSV
                     EKENDASSESGKDKTPATKPAKGEVESSSTTPTKVVSTTQNVAKPTTASSKTTKDVVQ
                     TSAGSSEAKDSAPLQKANIKNTNDGHTQSQNNKNTQENKAKSLPQTGEESNKDMTLPL
                     MALLALSSIVAFVLPRKRKN"
     gene            complement(14739..14912)
                     /gene="rpmF"
                     /locus_tag="NBH32_001286"
     CDS             complement(14739..14912)
                     /gene="rpmF"
                     /locus_tag="NBH32_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCD1514900.1"
                     /translation="MAVPKRRTSKTRKNKRRTHFKISVPGMTECPNCGEYKLSHRVCK
                     NCGSYNGEEVAAK"
     gene            complement(14992..15549)
                     /locus_tag="NBH32_001287"
     CDS             complement(14992..15549)
                     /locus_tag="NBH32_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HCD1514901.1"
                     /translation="MKWSITQLRKYQGKPFEFDQTVSFDNLKESLDLIDLSPITIQGQ
                     LTIKSTEVVADIHITGTYTMPCARTLVPVKVPLDVTTTEVFDLEGYNQYNDDQDDVDE
                     HYHIIKDGMVNLQDIVEDIVIIEKPMRAYSEQSDQMLTVGNGWEVIDEDQLDELAKQQ
                     EQDDSESRQVDPRLQKLQQLYDKEQ"
     gene            15676..16815
                     /locus_tag="NBH32_001288"
     CDS             15676..16815
                     /locus_tag="NBH32_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HCD1514902.1"
                     /translation="MKSVGLITEYNPFHNGHQYHINQSKKLTNADVTIAIMSGNFVMR
                     GEPAIYNKFTRAKMALSTADLVIELPATASLSSGDHFAELAVKVADYMSVDTIAFGSE
                     NNDIKTLKQLAHSINEIEQSESFSQKVKEGKSYPRIISELLEHHEALESPNNILGISY
                     LKAIAKHAKNINAISIKRENAQHHDSLIQHHQFASGTSIRTSIISQDDHWHHVVPKDI
                     QHLYVTPHITLNQIFPYLKYQIIAMTTESLKNIYTVTEGFENRLKSNIYEATDFHHFI
                     KLLKTKRYTYTHIQRLLMNVLLNIKPTDVTRNIHAVKVLAMNDRGRQYLKHLKTAFPE
                     RQYITNINKSNAHYFTNEIKATHIYNAISGQQQTDFNTPVIQQYR"
     gene            complement(16877..17359)
                     /gene="coaD"
                     /locus_tag="NBH32_001289"
     CDS             complement(16877..17359)
                     /gene="coaD"
                     /locus_tag="NBH32_001289"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCD1514903.1"
                     /translation="MEHTIAVIPGSFDPITYGHLDIIERSTDRFDEIHVCVLKNSKKE
                     GTFSLEERMDLIEQSVKHLPNVKVHQFSGLLVDYCEQVGAKTIIRGLRAVSDFEYELR
                     LTSMNKKLNNEIETLYMMSSTNYSFISSSIVKEVAAYRADISEFVPPYVEKALKKKFK
                     "
     gene            complement(17361..17903)
                     /gene="rsmD"
                     /locus_tag="NBH32_001290"
     CDS             complement(17361..17903)
                     /gene="rsmD"
                     /locus_tag="NBH32_001290"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002470236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HCD1514904.1"
                     /translation="MRVIAGKHKSKALESMEGRNTRPTMDKVKEGIFNSLYDVSGIGL
                     DLFAGSGALGIEALSRGMDKVIFVDQNFKAVKVIKSNLANLDLEAQSEVYKNNADRAL
                     KALSKRDIQFDVIFLDPPYNKGLIDKALKLISEFNLLKENGIIVCEFSNHEEIDYQPF
                     NMIKRYHYGLTDTLLLEKGE"
     gene            17973..18362
                     /locus_tag="NBH32_001291"
     CDS             17973..18362
                     /locus_tag="NBH32_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514905.1"
                     /translation="MKQTFITLGEGLTDLFEFMTMIEYNHQRIDKIIYFHSPQAENKK
                     SSVAIIMNPTTGNHFQAFYIMINAIKYPYPDSNKKFQMINDCAEKFDIPILGIDVQPP
                     QAFHDLSLYYNYLISVLRLQKWIPELQ"
     gene            complement(18365..18619)
                     /locus_tag="NBH32_001292"
     CDS             complement(18365..18619)
                     /locus_tag="NBH32_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HCD1514906.1"
                     /translation="MNLIPRTSIVVYLKHMKHERQIRKYGHIVHSNRDRKFVIMYVNE
                     QDVDQIVHKLMQLKYVRHIDGSPYKYLKKTYEKEKHEIYN"
     gene            18858..19784
                     /locus_tag="NBH32_001293"
     CDS             18858..19784
                     /locus_tag="NBH32_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1514907.1"
                     /translation="MKKVNRNILNAVLVGAGFVSSLLMINKNKVITKKQTIPAFFKGN
                     APYIFAHRGGMALRPEQTQLAFDYAKQLGVDGFETDVRLTKDQQLIVFHDATVDRTTN
                     GSGKVSAHTLAELKKLDAAYHFKDINGLTPYRGHAHTAILTFDELLKQYPDMYINVDL
                     KDAPESYEGSIAPQIMFDTIAENQAFDRVLVTSFYKEQIVRFNKIAQGSVAIGASQQE
                     VTEAFLKYHLLGGRYYQPLAQTFQMPTHFKGIDLTSSRFIKWLNDMNVIPGYYGVNSI
                     DLMNDLYQKGAHTIVTDRPDLAQQFKQTIPKK"
     gene            complement(19847..20281)
                     /locus_tag="NBH32_001294"
     CDS             complement(19847..20281)
                     /locus_tag="NBH32_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HCD1514908.1"
                     /translation="MINEASLAILDDIDELADMIVASDIYASFEQAKQALENNDEAHL
                     LYQSFLKSKEKYDEVMRFGKYHPDYKKVMLETRQRKRAYEMLDVVMHYKAREMALQHL
                     IDEVVTKIAYAVSEHVKIETGNPFFQTSHSGCTTGGSCNCSL"
     gene            complement(20297..21292)
                     /locus_tag="NBH32_001295"
     CDS             complement(20297..21292)
                     /locus_tag="NBH32_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002442198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD1514909.1"
                     /translation="MGFLIYLFYSPRLKFDVLENPNKGNKVNRSEQVNKSNNHAENPK
                     PKEGVGTWVGKNIKVLTSKFGQADRVYPFRDGYKNYVFKDKNSYYIVSTKREEIVSVY
                     ATGEKVNVSPLKIGQHSAEIFNHTSINPEPSFKVDGKKYEFELSDEDLKTQTLIKYGD
                     IYAQVYSDQQSKKVLSVRFLTKEMLADIEPYQLNSNSTSEEHNKRPVEQNPNQLISLY
                     EVTNEMRKLKGLKPLKINSDLAHIASNNLYEATSNGSDSVEFTEDALRGQLDKNHVTY
                     KTTAQNVGYAFNDVPTLIHSWMNSDIHRSRLLNSKYDEMGGDVMRDYYSLIFLEK"
BASE COUNT         7214 a         3845 c         2938 g         7296 t
ORIGIN      
        1 ctttttataa tgaaatgaaa taatgtaaag aattttctat tcaatacttt atcatgttta
       61 aattgtgtca ctataacatt ttcataaaca ttatacatga ccactatgta ttttgtaagt
      121 atccgcaatt aattctttac aacatatata aatgtttctg acgttattat catttatgat
      181 atgattattt ttgctaatga taatgaaatt atatgaaagg atttacacaa atggcgaata
      241 tcgtttttaa attgtcggat aaagacataa cgacattaat gtcacgaatt tcttttgata
      301 ctgagaattt acctcaaggt atgaaagcac gtgcaaagta tcaaaataca actgtaaata
      361 tttaccaatc aggtaaagtc atgtttcaag gtaatcatgc agaagctgtg tctcaagaac
      421 ttctaccgca gcattctcaa ttgaatacta ataaaactaa aaagaaaaat atggctaatt
      481 cttttcttga acagtcttta atgtatgatc aatttaactg tattggtagt gatgaggctg
      541 gaagtggcga ttactttggt ccattgactg tatgtgctgc ttttgttacc aaagagcatg
      601 tccttatttt aaaaactttg ggtgtagatg attcaaaaaa attaaccgat actaaaatcg
      661 tagaacttgc tgagcaattg gttactttca ttccacattc actattaaca ctacacaatg
      721 aaaaatataa tattcagcaa gcaaaaggct ggactcaagt aaaaatgaaa gctgttcttc
      781 ataatgaagc catcaaaaat gtacttgaaa aaattgattc gtctcaattg gattatattg
      841 tgattgatca gttcgcaaaa cgcgaagtgt atagccatta cgcattatca gatataccac
      901 ttccaaagaa aacaaaattt gaaacaaaag gagaatcaaa gtcattagcc attgcagtgg
      961 caagcataat ttctcgatac gcatttgtta catatatgga tcaaatatca aaaaatatta
     1021 atatgacgat tcctaaaggt gccggtgcta aagtcgatgt aatcgccgcc aaaattataa
     1081 aaaaatacgg tctttcacgt ttagatacca tttcaaaaaa gcattttaaa aatcgtgaaa
     1141 aagcccaaaa aattttaaag cctctttgat ttataatcat tagaggcttt tatgcagttg
     1201 tgcaaagcac caactgtaat ccgctgtgcc tctttgattt ttaatcaaga taaattggat
     1261 cctaatagga aatcctatta ggtaagtcat actaatttgc tttgctcata agtatgactt
     1321 tatatgcagt gatgcgaagc atcaactgta atccgctgtt cctctttgac tttttaaatg
     1381 cattggaacc taattggtga acccaaaaat aaaaccccat gttagggata tcaatctact
     1441 tcagatatcc ttatacatgg ggtttaaatc attatctaat aacagcacct tgttcaatta
     1501 atgctgcttc aatttccgct tgtacttttg aaacgcgctc atctgtcaat gtttcttctg
     1561 tgtctaaata atttaaacgt attgcaattg attttttacc tttttctaaa tgttcgccct
     1621 gatatacatc aaagacaagt gtatctttta atatattgcc accgtgtgca tgaatcgttg
     1681 ataataaatc agctgctgga atattttgat ctacttctaa tgcaatgtca cgagacatgc
     1741 ctgggaatct cggaattggc tggtaattaa tgtaacctac cgacacagcc attaatgcat
     1801 caaaattcaa ctcaaaaaca tacgtacgtt ttaaatcatt atcagctgct aatgttggat
     1861 gtaattcacc aataaaacca acaactttat tctctaataa gatttcagca gtacgacctg
     1921 gatgtaatcc atcaatatca gcacgacgat aactaaattc aaggtttaac ttttcagata
     1981 ctcgatccac gacacctttt gctaaataga aatcaaccgt ttctttctta ccttgccatt
     2041 gattgactac ataatctcca gttaaaatac cacttaaata ttcaacttga tctggtagtt
     2101 caccttctcc attagcaaag aagacattgc cgatttcaaa taattttaca tctttatttt
     2161 tgcgtgccac attatatgat gccgcttcga ttaaatgtgg taataaactt tgacgtaatg
     2221 acgcatgcgc ttcactcatt ggcatcaata aatcaattgt ttgacgctgt tgcatcgcaa
     2281 atgcagtagc atcttcttta gaaactaacg aatacgtaat agcttggtct aatccagcac
     2341 cttctaacac ttctttaacc attctcgttt tatattggcg atcagttagc tgaccactag
     2401 taactttttc gaagacaggt aacgttgatg gaatatcgtc gtacccataa atacgtgcaa
     2461 cttcttcaat taaatcttct ttaattgtaa tatctttacg acgtgatggt acttgcactg
     2521 taataacatc atcatttatt tctgtatcaa accctagttg attaaaaata gtaacaatat
     2581 cattttgtga caaatcaaat ccaatagtgc gattaatttt atcagcagtg atgtcgattg
     2641 gtgtaataaa tgcaccaagt tctcctgaag acactctatc ttttagcact tttccgtttg
     2701 cataagtttg taataaataa catgcacggt cgactgcttc atctacaaat tcagtagcta
     2761 ttcctttttc aaaacgacta gatgattcac tgcgtaaatt taaacgtctt gatgtatgac
     2821 gaattgaaac tggatcaaaa atagcacctt caatcactat atttgatgta tgttctttaa
     2881 cttctgaaaa atcgccaccc ataacaccag ctaatgcaat tggagtttgt ccattagtaa
     2941 tgacaatatc gctcgttaat aattcacgtt ctgtatcatc taatgttgtc attttttcgc
     3001 cttcattcgc ttgacgaaca acaatttgtt gtgaaccaat cgcatcttga tcaaacatgt
     3061 gcaatggttg accgtattct aataacacat aatttgaaat gtcaacaaca ttattaatag
     3121 gacgtatacc cgcttttatt aagcgtgctt gcatccaaat tggcgaaggt tcaattgtca
     3181 cgtcgtgaac aacacgtgca ctataatatg gtactttatc ttcattttca attgtcacag
     3241 tcagttcatc atttgcagat aactcaagct catttgatgt tgtgtctggc ttagtcattt
     3301 ttgtattata taatgctgca acttcataag cagtacctat catacttaaa gcatcagcac
     3361 gattcggcgt taaatcaaat tccattactt gatcatctaa atataaagct tgtaaggcat
     3421 ctgttcctgg aacttgggat tcactaaaaa cataaatgcc tgattcaaaa cttttcggta
     3481 catagttact tgaaatacca atttcttgta acgaacaaat cataccttct gaacgttcac
     3541 cgcgtaattt ggcacgctta attttaatac caccaggcaa tcttccacct actttagcaa
     3601 caatgacata ttgtcctgca tcaacgttcg gtgcaccaca tacgatttgt acaggttcgt
     3661 cttctccgat atcaacttgg caaacattta atttatcagc atcaggatgt ttctcttttg
     3721 acttaacgaa gccgacaact aaatttttga tatcttttgt gtagtcaatt aaatcatcca
     3781 cttcaatacc tgtgcgcgta atacgttctg ccaaattact tacagaatca tcgattgtta
     3841 catattcttt caaccattca tttgatatca acattacatg tcacctctat cttctaccgc
     3901 tttaaattga tctaaaaatc tcacatcatt agtatagaaa tgacgaatat cttctatacc
     3961 atattttaac attgcaatac ggtctggtcc cataccaaat gcaaatccag agtactctga
     4021 agaatcaaaa ccagccattt ctaatacatt aggatgtacc attccagcac ctaaaatctc
     4081 aatccatcct gtgtgtttac acacattaca accttttcct ttacatttaa aacatgacac
     4141 atcaacttct acagaaggtt cagtgaatgg gaagtaactt ggacgtaaac gaatttcacg
     4201 atcagcacca aataatttct tagctaacaa ttctaaagta cctttcaaat cactcatttt
     4261 aacgttttta tcaacaacta atccttcaat ttgtgtaaat tgatgactat gtgtcgcatc
     4321 atcagagtca cgacgataca ctttaccagg gcaaataatt ttaactggac cttgaccatg
     4381 acgtgattcc atcgtacgtg cctgcactgg tgatgtatgc gtacgtaata aaatttcatc
     4441 cgtaatatag aaactatctt gcatatcacg tgcagggtgt gatttaggta aattcagcat
     4501 ttcgaagtta taatgatctt gttcaacttc atatccattc acaatttcat aacctaaacc
     4561 taagaataag tcttcaattt cttctattgt acgtgttaat ggatgctttg aaccgatttc
     4621 aatatgacga cctggtaatg atacatcaat tgtttcttca gccaattgct tatttaattt
     4681 ttctttaact aacatctgtt gtctttcatc taattcattt tgaattgttt gacgcaattc
     4741 attcactttt tgaccaaatg caggtttctc ttcattcggc aaatccttca tcaatttcat
     4801 tagtccgcta actgaccctt ttttacctaa gtatttcact ttaacttctt gcagtgcacg
     4861 ttcatcattt gcttcattaa tatctacaag cgcttgttgt tttaactctg acattgtttg
     4921 ttgttcagac atatgtcact gacctcccta ttcgaactat tcattttcta tttatcatat
     4981 ttgttgttta actaaaaaaa gactttcatc ctcttaaaaa ataagggacg aaagtcattt
     5041 ccgtggtacc acccaaattt atgcctaagc atacacttgt ttaatattga taacggattg
     5101 tctttccgac ttaaatctct ttaagtaacc aaaaaggtga aaagacttta aataattgga
     5161 ataacttgca gtcacgatta ttctccctaa aacaattatt tatataaatg tccatgcctt
     5221 tttattggtt tatattcaat tgtgtatgca atcacgtaga aatgttctgt taaacaatta
     5281 attataatta tatcggaaaa ctttcaacac ggtcaacctt tcaaatgata aagtaaaata
     5341 ctacctgcaa tcgctacatt taaactttca gctttaccat aaataggtat gattaaattt
     5401 tgtgtagtat gtgctaataa ttcaggatta acaccttctc cctcattacc taatagtaat
     5461 gcaaaagaat cacttgaagt aacttctttg tatgccactg cgttttcaag tgctgtacca
     5521 taaacaggac cattaaattg agtaataaac gtatcgagat cttgagtcat aactggcaaa
     5581 tgaaaaacac taccttgact cgctcgcaat actttatctt gataaggatc tgtcgtaccc
     5641 ttctccatta ttacagcatc cattccagca gcatccgcag ttctaattaa tgtgcctaaa
     5701 tttccaggat cttgaacacg atcgataagc aatacttgtt gtgctttaga aatatcatat
     5761 tgtggcttct caatgattgc gaaaaaccct tgtggtgtca ctgtacctga taaagattca
     5821 gcaactttca tgtttatttc aaaaacttct tgcgcgtatg cgataatttg ctgatctaat
     5881 cttgccggtt caattgcaaa taattgtgta attacaatcc cactttgata agcttcttca
     5941 attaaatgca caccttcaat taaagctaat ccagttttat ccctctcacg tttctttttt
     6001 agcttgttcg cttgtttaat tctattattt tgtgcagaag taatttgttc catagatagc
     6061 tcctcgcttt atttttaaaa ataaaaatat taatcattaa taagatgaaa acatttgatt
     6121 gtatagttaa tattaattaa tcgattttat cactcatatt atttcaaatt gtataaattt
     6181 cttttatcga tactactact ataaatcata cgccccaaaa tatcattatt aattcttttc
     6241 ttcttcaaaa taaatcaaaa tgatataatt gatgattatt ttcaaagcac attcaaatca
     6301 aactacgctt tagcaatttg ttgttagcat gtttgtgttc attaaaaaaa cgaccatcat
     6361 cggtatcatg tatggtcgtt acaaaagcta acaataccaa ttgtcataac aagtactgca
     6421 acctctttaa attcaattat ttcatgtaac tatagcctat atcatatgta attactttgt
     6481 tattgataat cgggctactt tcatcttcat ttttacttct aacatgttta tgtgctgctt
     6541 taaagacatc agattttaac caatccgtaa aagcttgctt tgatttccaa actgttaaaa
     6601 ttttcacttc atcaaaatct tcttgttcta aagtttgtgt aacaaacatg ccatcaaagc
     6661 cttctaatgt ttcaatccca tgtctcgtgt aaaatcgttc tataatatct tttgctgttc
     6721 cttttgttaa cgtcagccta ttttctgcca tgaatttcat aattatcctc ttttctgttt
     6781 aacttacctt aattattttt gcgacaacaa caattctttt cgttgtttca ctatatgcat
     6841 cttcgcacgt tgataaagtt attattttat cttttaccgt tacattaaca tctgaattaa
     6901 ttacagattt acgttttgtc tcatctaaaa attgttgata atcttgatca ttttcaaaat
     6961 ctgtacgtat gtaattatct ttagtagtag ttttatatgc actaaatact tgcaattgat
     7021 atttaccata tttattgtca aattcaatta tcttgtgttt ttcataaaaa gattgcttta
     7081 aataatcttc taacacatca aacatcgtat tatcaccgac atggtgccca tataaaatag
     7141 tattatgatt taaattcttc aattcatttc taaaatccat aaaaatacta cctttacgtc
     7201 gatgttctcg ctcaaaatct aaatttaaat aatcgtgatt tgtcttacct tgtagtactg
     7261 gataatttaa tgatgttcct gataatttta tccatccaac aatgtcttta tttatttttt
     7321 caagtgattc aaattgtggt ctcacatgtg cttgatgttt gctcatcagc atttgaaatt
     7381 tttgttgtaa tttctcataa tttgcgcgtt cttgcttgtc ttcaatatat gtttgaacaa
     7441 ttttgtaacc aaaaatgata ataattacaa ccaataaaat ttgtacaata gttaaaaatc
     7501 gcttcattct cataaaaatc ctcttttatt aacgacgttt cttcagtcac tactaaacca
     7561 gttattgtac cgttttagat tcgatttcgt tgactttgac aaattaagta aattagcatt
     7621 ggaccaccga caatcattaa aatagcattg gctggaattt ctaaaggagg ctgtatcact
     7681 cgtcctaata aatcagccac taacaatagc catgcaccaa taactgtaga aaatggaata
     7741 agtactctgt aattgccccc aactagcttt ctaaccacat gtggcacaat aatacctaaa
     7801 aaggctagtt gtccaacaat cgcaacagtt gcacttgcta aaaatactgc taataaacct
     7861 gttaaccatc tgtaacgatc aatattgaaa ccgatacttc gcgcttgtac gtcatctaaa
     7921 tttagtaaat tcaatttagt ggacaatagt aatgttaata ttaatcccaa taatgctgat
     7981 actgctaata tgtatacgtc gctccatatt ttcattgtta agccttgagg aattttcatt
     8041 aaagggtttt gagttaaaat ttctaaaaca ccatttaata atacgaataa cgcaacacct
     8101 actaatatca tacttacagc attgaatcta aatttagaat gcaacaatat aattattaaa
     8161 aatggtatta aacctccaat aaaacttaat aatggtaagt aaaagtacaa ttgtggaata
     8221 aacaacatac aaagtgctct cattataagt gcacctgaag aaacgccaat gatattcgcc
     8281 tctgccaaag gattttgtag tgctgcttgt aataatgctc cagaaactgc taacattgcg
     8341 ccaaccatta atgcaattaa tatacgtggc aatcgcaaat caatgattga atccactgct
     8401 tcattgctac cagttgtaaa ttttgtaaat aggtcattaa atgacaattt aattgtaccg
     8461 gttacaaacg aaatataagc agttgcgatt aaaatgacta acaaacataa aaatagtagt
     8521 ttctttttat tttttatcat catatcaact cactattttt tatccttata aaataaatca
     8581 tataattgag tcattgcctt atcagcatca acatttgctg taataccgaa tggcacttcc
     8641 tctaagtcat aaacatgatt atttttcaca gctttaaaat gtttccaaat atcattttgt
     8701 ttaaattctt tttgaaacat tttcttaact tcttcaggca ttccgtgtgg taatcgtaaa
     8761 ataatatctg gatttatatt caacaaattt tcagtattag acgaaatata ttgacgatct
     8821 ttcactttaa taacattttc tccacctgct attttaacta aatcaccaat atatgattta
     8881 tcagttgcta ctaaatagct accaggtaca cccattaata ttaatacttt gggatgtttc
     8941 ttttgtttag ctgctttatt ttcaattttt tgctttacag aatttaaatg gtcatttaat
     9001 tcttttgctt gtgctttacg atcaaattga tcacctaatt gtgtaatcga cttttgcatc
     9061 ccttttaaac tatcaaaatc ataaaagtag cctttcatat ttaattgttt gtaaaatggt
     9121 tgcatttcat ctttaatcgt tgacacactc aaaacatgtg ttggttttaa ctttttaaca
     9181 gcttcaacat tcggctccat tggttgacca atttccggta catctttata gcgattaggc
     9241 aatgtcttat atgacgtggg tttgccgaca attggtaaat ccaatttgtc caatgtcatt
     9301 gtcaatgcga cagttgttgg tacgattctg aattcgccgg atttagttga ttcttgagaa
     9361 ctggaagatt gacagctggt taagataacg acgcttatca ctaaaatggt taaatacttt
     9421 atgattctca aacttggtta cctctctttc tgctgtataa atgtgccgaa ataagtaaaa
     9481 tggttgctaa cacaatgcca gctgcaaata acatatattg ctttttatga tttggttcat
     9541 caagttcttt aatatcacta gataattgac tactattctt gctatcatca tctctattaa
     9601 aagctggtaa cgctttttct ttttcaacta cttttttctt ttcctttttt gatttttcat
     9661 ctttatgttt cggttgctct ttgtgattat tttcaacctt ttttactgtt gaaggattat
     9721 tttcaacttt taatgtacct gatgctttag gtcgctcatt ttcaatcttt ttattactgt
     9781 cttgttgcac tttatctttt tgtacaggaa tatcatttaa gcctttttca gtaaaaaggc
     9841 tagtacgtct ttccttaatg tctgattttt gactatcttt tttgtcatca atatcttttt
     9901 taagctgatc ttgattattt tcttgacttt gatgttttga aggcacttct tttgcatcat
     9961 catgttgtac ttttttctct ttaaaatgaa tagaatacac catttctttg ttatctagat
    10021 ttttcttttc agccaacgtt ttaacatgca tattgatttt atccatatgc tgttcaatta
    10081 aaattcttac tattgtgtca tccttttcat cttttataac aaaaggaaat tcttcatttt
    10141 tgtcattttg aaaaacaagt tgttctttat caatttgacc ttttagcttt acttgcaagt
    10201 aaattttacc agtcatatct gaaatcaatt gcgccttttc gtcaactagt tgttgttcct
    10261 cagttacatt tttattttct tgattaaaaa ttactaactc taacgcttta ttctctttta
    10321 tcttttcatc aaaaattgat gtagttaatt ctgttttatc ttttgcttca ggttgattaa
    10381 atttggctga tggatatttt atctcatcag ccaacacgct accaatcatt gttgtatatg
    10441 taagtatacc taagctaata atagctataa tagattttgt agcaatttgt ttaacatttc
    10501 tcattattcc acattgcctt tagatttttt tctaacaaac aatgtgattg cgattaataa
    10561 tgctaaggat gcaactggta ttgcatatat atatgcaggt gtaccagcat ttgtttgtgg
    10621 attttctact ttgtctttat tcgaactgtt agattcactt tgaccagtag tcgttccatc
    10681 agatcctttg tcactacctg aagcagaatt tttatcatct ttacctggtg cattagcacc
    10741 tgctacatca gttggtccat taaatttata tgtaatgttg taatgatggt catatttgaa
    10801 tggcttacca tttacttttt catcgatata aacgtcaatt tttccatcta ttttaccgtt
    10861 caacttactt acttcaaatt cagaagtgcg ttcatctttg gcagtgtttt tactaataat
    10921 attttcttta tgtccttcga tactcattcc agtaatccaa tgactgtggt tgacagttat
    10981 ttgaacatac aatttaccat ttttcttaat gtactttgcc ggtttattaa aatagtcatt
    11041 agcaattgac gtgtcattgg tattgtattt gtaaacctca taattcaaag taccgctatc
    11101 tgcggcattt gcagaattac tgaatgtcgc gatgatgata attaacgcta aaatcgttgt
    11161 attaaaaact tttaaaatat ttttcaaaac ataatcctcc tttttatgat tgcttttaag
    11221 tctttactaa aatcataaat aataatgatt atcattgtca atatttattt tataatcaat
    11281 ttattattgt tatacgaaaa tagatgtgct agtataattg ataaccatta tcaattgcaa
    11341 tggttaatca tctcatataa caacacataa tttgtatcct taggaggaaa acaacatgac
    11401 aaaacattat ttaaacagta agtatcaatc agaacaacgt tcatcagcta tgaaaaagat
    11461 tacaatgggt acagcatcta tcattttagg ttcccttgta tacataggcg cagacagcca
    11521 acaagtcaat gcggcaacag aagctacgaa cgcaactaat aatcaaagca cacaagtttc
    11581 tcaagcaaca tcacaaccaa ttaatttcca agtgcaaaaa gatggctctt cagagaagtc
    11641 acacatggat gactatatgc aacaccctgg taaagtaatt aaacaaaata ataaatatta
    11701 tttccaaacc gtgttaaaca atgcatcatt ctggaaagaa tacaaatttt acaatgcaaa
    11761 caatcaagaa ttagcaacaa ctgttgttaa cgataataaa aaagcggata ctagaacaat
    11821 caatgttgca gttgaacctg gatataagag cttaactact aaagtacata ttgtcgtgcc
    11881 acaaattaat tacaatcata gatatactac gcatttggaa tttgaaaaag caattcctac
    11941 attagctgac gcagcaaaac caaacaatgt taaaccggtt caaccaaaac ctgctcaacc
    12001 taaaacacct actgagcaaa ctaaaccagt tcaacctaaa gttgaaaaag ttaaacctac
    12061 tgtaactaca acaagcaaag ttgaagacaa tcactctact aaagttgtaa gtactgatac
    12121 aacaaaagat caaagtcaaa cacaaagtgc tcgtacagtg aaaacaacac aaacagctca
    12181 agaacaaaat aaagttcaaa cacctgttaa agatgttgca acagcgaaat ctgaaagcaa
    12241 cagtcaagct gtaagtgaca ataaatcaca acaaactaac aaagttacaa aacaaaacga
    12301 aacgcctaaa caagcatcta aagctaaaga attaccaaaa actggtttaa cttcagttga
    12361 taactttatt agcacagttg ccttcgcaac acttgccctt ttaggttcat tatctttatt
    12421 acttttcaaa agaaaagaat ctaaataaat catcgtcaca ctcataactt aatatatttt
    12481 ttattttaaa ttttatttaa cctatgtcat agatatttca taatctataa cataggttat
    12541 ttttttataa aataacgttg caattaacta ccatttcaat gtacaataca agtaatcaat
    12601 tgataatgat tatcagttga taatatacaa ttaggagttg tttctacaac atgaacaaac
    12661 agcaaaaaga atttaaatca ttttattcaa ttagaaagtc atcactaggc gttgcatctg
    12721 tagcaattag tacactttta ttattaatgt caaatggcga agcacaagca gctgaagaaa
    12781 caggtggtac aaatacagaa gcacaaccaa aaactgaagc acttgcaagt ccaacaacaa
    12841 caactgaaaa agctccagaa actaaaccag tagctaatgc tgtctcagta tctaataaag
    12901 aagttgaggc ccctacttct gaaacaaaag aagctaaaga agttaaagaa gttaaagccc
    12961 ctaaggaaac aaaagcagtt aaaccagcag caaaagccga taacaataca tatcctattt
    13021 tgaatcagga acttagagaa gcgattaaaa accctgcaat aaaagataaa gatcatagcg
    13081 caccaaactc tcgtccaatt gattttgaaa tgaaaaaaga aaatggtgag caacaatttt
    13141 atcattatgc cagctctgtt aaacctgcta gagttatttt cactgattca aaaccagaaa
    13201 ttgaattagg attacaatca ggtcaatttt ggagaaaatt tgaagtttat gagggtgaca
    13261 aaaagttgcc aattaaatta gtatcatacg atactgttaa agattatgct tacattcgct
    13321 tctctgtatc aaatggaaca aaagctgtta aaattgttag ttcaacacac ttcaataaca
    13381 aagaagaaaa atacgattac acattaatgg aattcgcaca accaatttat aacagtgcag
    13441 ataaattcaa aactgaagaa gattataaag ctgaaaaatt attagcacca tataaaaaag
    13501 cgaaaacact agaaagacaa gtttatgaat taaataaaat tcaagataaa cttcctgaaa
    13561 aattaaaggc tgagtacaag aagaaattag aggatacaaa gaaagcttta gatgagcaag
    13621 tgaaatcagc tattactgaa ttccaaaatg tacaaccaac aaatgaaaaa atgactgatt
    13681 tacaagatac aaaatatgtt gtttatgaaa gtgttgagaa taacgaatct atgatggata
    13741 cttttgttaa acaccctatt aaaacaggta tgcttaacgg caaaaaatat atggtcatgg
    13801 aaactactaa tgacgattac tggaaagatt tcatggttga aggccaacgt gttagaacta
    13861 taagcaaaga tgctataaat aatactagaa caattatttt cccatatgtt gaaggtaaaa
    13921 ctctatatga tgctatcgtt aaagttcacg taaaaacgat tgattatgat ggacaatacc
    13981 atgtcagaat cgttgataaa gaagcattta caaaagccaa taccgataaa tctaacaaaa
    14041 aagaacaaca agataactca gctaagaagg aagctactcc agctacgcct agcaaaccaa
    14101 caccatcacc tgttgaaaaa gaatcacaaa aacaagacag ccaaaaagat gacaataaac
    14161 aattaccaag tgttgaaaaa gaaaatgacg catctagtga gtcaggtaaa gacaaaacgc
    14221 ctgctacaaa accagctaaa ggtgaagtag aatcaagtag tacaactcca actaaggtag
    14281 tatctacgac tcaaaatgtt gcaaaaccaa caactgcttc atcaaaaaca acaaaagatg
    14341 ttgttcaaac ttcagcaggt tctagcgaag caaaagatag tgctccatta caaaaagcaa
    14401 acattaaaaa cacaaatgat ggacacactc aaagccaaaa caataaaaat acacaagaaa
    14461 ataaagcaaa atcattacca caaactggtg aagaatcaaa taaagatatg acattaccat
    14521 taatggcatt attagcttta agtagcatcg ttgcattcgt attacctaga aaacgtaaaa
    14581 actaataaat cgtctttata tttaattatt aaattaacaa attttaattg acggatgagg
    14641 tatccagtta cctcgttcgc caattatttt tcgcaatata aaaagtccca cttaaaacaa
    14701 tcattttaag cgggactttt tatattgagt aactaaaatt atttagctgc tacttcttcg
    14761 ccattgtaag aaccacagtt tttacataca cggtgtgata atttgtattc gccacagttt
    14821 gggcattcag tcatacctgg tactgaaatt ttgaaatgcg tacgacgttt gttttttcta
    14881 gttttagaag ttcttctttt tggtactgcc atgatatatc ctccttagat tataaacgaa
    14941 aaatactaaa tgttagttta attaacaaca ttatatcatt aattaaacta cttattgctc
    15001 tttatcatat aattgttgta atttttgaag ccttggatca acttgtcgtg attctgaatc
    15061 atcttgttct tgctgtttag caagctcatc taattgatcc tcatcgatta cttcccaacc
    15121 attacctact gtcaacattt ggtcactttg ctctgaataa gctctcattg gtttctcaat
    15181 aataactata tcctcgacaa tatcctgaag attaaccata ccatctttaa taatgtgata
    15241 gtgttcatct acatcatctt gatcatcgtt atactgattg tacccttcta aatcaaatac
    15301 ttctgtagta gttacatcta gtgggacttt aactggtaca agagtacgtg cacaaggcat
    15361 tgtatacgtt ccagtaatgt gaatatccgc aacgacttct gttgacttaa tggttaactg
    15421 accttggatt gtaattggag ataaatcaat taaatctaat gattctttta aattgtcaaa
    15481 actcaccgtt tgatcaaatt caaatggctt accttgatat ttccttaatt gcgtaattga
    15541 ccatttcata tggcttcacc tctaacaagc acaaaattta ttttaacttt ctaagaacaa
    15601 gttgtcaaga ttttttctta acatatcatg ttctgttaca atattaataa tgaataccga
    15661 aaggacgaat tgcaaatgaa aagcgttggc ttaatcacag aatataatcc ctttcataat
    15721 gggcatcaat atcatattaa tcaatctaaa aaacttacaa atgctgacgt tactattgca
    15781 ataatgagtg gtaactttgt catgcgtggc gaaccggcaa tctataataa gtttactcgt
    15841 gcaaaaatgg cattatcaac agctgattta gttatcgaac taccagcaac tgccagttta
    15901 tcatctggcg atcattttgc cgaactagca gttaaagtcg cagattatat gagtgtcgat
    15961 acaattgcat ttggtagtga aaataatgat atcaaaacat taaagcaatt agctcacagc
    16021 attaatgaaa ttgaacaatc cgaatccttt tcacaaaaag taaaagaagg taaaagctac
    16081 cctcgtatca ttagcgaatt attagagcat catgaagcac tagaaagtcc taacaacata
    16141 cttggtatta gttatctgaa agcaattgct aaacatgcta aaaacatcaa tgcaatttct
    16201 attaaacgag aaaatgctca acatcatgat tcattaattc aacaccatca gtttgcaagt
    16261 ggtacatcta ttagaacatc aatcattagt caagatgatc attggcatca tgtggtacct
    16321 aaagatattc aacacctgta cgtaacacct catattacat taaatcaaat atttccatat
    16381 ttgaaatatc aaatcatagc tatgactaca gagtcattaa aaaatattta tactgttact
    16441 gaaggttttg aaaatcgttt gaaatctaac atttatgaag caactgattt tcatcatttt
    16501 ataaaattac taaaaacaaa gcgttataca tatacgcata tccaaagact gttaatgaat
    16561 gtattattaa acattaaacc tacagatgtt acgagaaaca ttcacgcagt taaagtatta
    16621 gcgatgaacg accgtggcag acaatattta aagcatttaa aaacagcgtt cccagaaaga
    16681 caatatataa ccaatattaa caaaagcaat gcacattact tcacaaatga aatcaaagca
    16741 acacacatat acaatgcaat aagcgggcaa caacaaacag atttcaacac gcctgtcata
    16801 cagcaatatc gttaacaaat gaaaacagta ttttaggatt gtaaaccatg ataaacctaa
    16861 aatactgtta tttttattac ttaaatttct tcttcaatgc cttttcaaca taaggtggaa
    16921 cgaattcaga aatatctgct cgataagctg caacttcttt aacaatactt gaacttataa
    16981 atgaataatt agtactagac atcatatata acgtttcaat ttcattgttc aactttttat
    17041 tcattgaagt taagcgtaat tcatattcaa aatcactgac tgctcttaaa ccacgtatga
    17101 ttgttttagc tcctacttgt tcacaataat cgactagtaa accactaaat tgatgaacct
    17161 tgacattagg taaatgttta acagattgtt caattaaatc catacgctct tctaaactaa
    17221 acgtaccttc ttttttacta tttttaagaa cacagacatg aatttcatca aatctatctg
    17281 tacttctctc aataatgtct aaatgaccat aagtaatggg gtcaaaacta cccggaatga
    17341 ccgctattgt atgttccatg ctattctccc ttttctaata acaatgtgtc tgtcaaccca
    17401 taatggtaac gtttaatcat attaaacggt tgatagtcta tttcttcatg attgctaaat
    17461 tcacaaacga tgataccatt ttctttcaat aaattaaact ctgaaattag ttttaaagct
    17521 ttatcaatga gacctttatt ataaggtgga tctaagaaaa tgacatcaaa ttgaatatca
    17581 cgttttgaca atgcttttaa agctctatct gcattatttt tataaacttc agattgcgcc
    17641 tctaaatcca aattcgcaag atttgattta ataactttta cagctttaaa attctgatca
    17701 acaaagataa ccttatccat acctcgagag agtgcttcta ttccaagcgc cccgcttcct
    17761 gcaaataaat ctaaacctat acctgacaca tcatataaac tattaaagat gccttcttta
    17821 actttatcca tagttggtct cgtattacgg ccttccatac tttctaaagc tttactttta
    17881 tgtttacctg caatgacgcg catgttgttc acacttccaa ttcatttagt tatttaatat
    17941 aatttattga gaaaaaggag aatgataaac caatgaaaca aacatttatt acactaggtg
    18001 aaggtctaac agatttgttc gaattcatga cgatgattga atataaccat caacgtattg
    18061 ataaaattat ctattttcat tcaccacaag ctgaaaataa aaagtcatct gtagcaatca
    18121 ttatgaatcc tacaactggc aatcatttcc aagcatttta tatcatgata aacgctatta
    18181 aatatccata tccagattca aataaaaagt ttcaaatgat taatgattgt gctgaaaaat
    18241 tcgacatacc tattttaggt atcgatgtac agccccctca agcatttcat gatttatcgt
    18301 tatattataa ttatttaatt agtgtgttaa ggctccaaaa atggatacca gaacttcaat
    18361 aatattaatt gtatatttcg tgtttctctt tttcgtaagt tttctttaag tatttatatg
    18421 gtgagccatc aatgtgtcta acgtatttaa gttgcattag tttatgtaca atttgatcaa
    18481 catcttgctc attcacatac ataattacaa atttacgatc tctatttgaa tgaacgatat
    18541 gtccatattt tcggatttgt cgttcatgtt tcatatgttt taaataaact acaatactag
    18601 ttcttgggat taaattcatt taaacctcac tccatttatt ttcagcatta atcatatcat
    18661 gtctttatta attttaaaat attaagctac cttaattatg tcttatgtaa ttgctcgata
    18721 aatgtattta gattatatta aacatcatta tattaattca attctcaaga acaccactat
    18781 atgattaacc acacatttcg ctgtagtcag aactatgaat ttgttaagct ataaaagtaa
    18841 acgaggagga attttttatg aaaaaagtaa acagaaacat tttaaatgcg gtactagtcg
    18901 gtgctggatt tgtaagtagt ttattaatga taaataaaaa taaagtgatt actaaaaagc
    18961 aaaccatccc tgcttttttc aaaggcaatg caccatatat atttgcgcat cgcggtggta
    19021 tggctttaag accagagcaa acgcaacttg catttgacta tgctaaacaa ttaggcgttg
    19081 atggctttga aacagatgtg agactcacaa aagatcaaca gcttatcgtt tttcatgacg
    19141 caactgttga tagaacgacg aatggctctg gaaaagttag tgcccataca ttagcagaat
    19201 taaagaaatt agacgcagct tatcacttca aagatatcaa tggactaaca ccatatcgtg
    19261 gtcatgcaca cacagctata ctaaccttcg atgagttgct gaagcaatat cctgatatgt
    19321 atataaacgt agacttaaaa gatgctcctg aatcctatga aggttctatc gcaccacaaa
    19381 ttatgtttga tacaattgca gagaatcaag catttgaccg cgttcttgtg acaagttttt
    19441 ataaagaaca aatcgtccgt tttaataaaa ttgcacaagg atccgttgca attggtgcta
    19501 gccaacagga agttacagag gcatttttaa aatatcattt attaggtggc aggtactacc
    19561 aaccattagc acaaacgttc caaatgccta ctcattttaa aggcattgat ttgacttcat
    19621 ctcgttttat caaatggtta aatgatatga atgtcattcc aggctattat ggcgtaaata
    19681 gtatagatct gatgaatgat ttataccaaa aaggtgcaca cacgattgtg acagatcgac
    19741 cagatttagc acaacaattc aaacaaacaa tcccgaaaaa ataaaaacat cgagtcagat
    19801 gctagttagt ggtttcaata accatctttt tctgactcga tgttttttat aatgaacaat
    19861 tacaggatcc gcccgtcgta caaccactat gtgatgtttg aaagaatgga ttacctgttt
    19921 ctattttgac atgctctgaa acagcgtacg caattttcgt aacaacttca tcaattaaat
    19981 gttgaagtgc catttcccta gctttataat gcatcacaac gtcaagcatc tcatatgctc
    20041 ttttgcgttg tcttgtctct aacataactt tcttataatc aggatgatat ttcccgaatc
    20101 gcattacttc atcatatttc tcttttgatt ttaaaaatga ttgataaagt aaatgtgctt
    20161 cgtcattgtt ttcgagcgcc tgtttagctt gctcaaatga tgcataaata tctgatgcaa
    20221 ctatcatatc agctagttca tcaatatcat ctaatatcgc tagtgaagct tcattaatca
    20281 taaatacccc tcattgttat ttttctaaga aaattagtga ataataatct ctcattacat
    20341 ctccacccat ctcatcgtat tttgaattta atagacgaga tcgatgtata tctgaattca
    20401 tccaactatg gattaatgtt ggtacatcat taaacgcata accaacattt tgagcagttg
    20461 ttttataagt aacgtgattt ttatctaatt gccctcttaa tgcgtcctct gtaaattcaa
    20521 cactatcaga accattagag gtcgcttcat ataagttatt agatgcaata tgtgctaaat
    20581 cgctattgat tttcaatggt tttaatcctt ttaattttct catttcattc gttacttcat
    20641 aaagagaaat taattgattt ggattttgct caactggacg cttattatgc tcttctgacg
    20701 tagaattaga atttaattga taaggttcaa tatctgctaa catttctttt gttaaaaatc
    20761 gtacacttag cacctttttc gattgttgat cagaatacac ttgtgcatat atgtcgccat
    20821 atttaatcag tgtttgtgtt tttaaatctt catctgaaag ttcaaattca tattttttac
    20881 catcaacttt aaaggacggt tctggattaa tacttgtatg attaaaaatt tctgcagaat
    20941 gttgtcctat ttttaacgga ctaacattga ctttctcacc tgtagcatac actgaaacga
    21001 tttcttcacg tttagttgaa acaatgtaat aactgttttt gtctttaaac acataatttt
    21061 tgtaaccatc tctaaaaggg tagacacgat ctgcttgtcc aaatttagaa gtaagcactt
    21121 taatattttt acctacccat gtaccaacac cttctttagg ctttggattt tcagcatggt
    21181 tatttgattt attcacctgt tcagatctat ttactttatt acctttattc gggttctcta
    21241 atacatcaaa ttttaatctc ggtgaataaa aaagatatat taaaaatcca att
//
LOCUS       DAHHOJ010000041        20823 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.041, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000041 DAHHOJ010000000
VERSION     DAHHOJ010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20823)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20823)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20823
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.041"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            337..837
                     /locus_tag="NBH32_001296"
     CDS             337..837
                     /locus_tag="NBH32_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1514910.1"
                     /translation="MTFEEKLSEMYNEIANEISSMIPVEWEKVYAIAYVDDQGGEVVF
                     NYTKPGSDELNYYTNISRGYNVSEEIFDDLWMNLYYLFKNLRNLFKEEGLEPWTSCEF
                     DFTRDGKLKVSFDYIDWINTEFDQLGRENYYMYKKFGVLPEMEYEMEEIKEIEQYIKE
                     QDEAEL"
     gene            848..1336
                     /locus_tag="NBH32_001297"
     CDS             848..1336
                     /locus_tag="NBH32_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001008490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01741 family protein"
                     /protein_id="HCD1514911.1"
                     /translation="MTFEEKLSQMYNEIANEISSMIPVEWEKVYVIAYVDDGGGEVIF
                     NYTKPGSDELNYYTYIPREYNVSEKVFYDLWTDLYRLFKKLRNAFKEEGLEPWTSCEF
                     DFARDGKLNVSFDYIDWVNTEFDQLGRENYYMYKKFGVLPETEYEMEEIRAVEKYVKE
                     QE"
     gene            1959..2333
                     /locus_tag="NBH32_001298"
     CDS             1959..2333
                     /locus_tag="NBH32_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD1514912.1"
                     /translation="MKRILVVFLMLAIVLAGCSNKGEKYQKDIDKVYKKQNQMNKIAS
                     KVQNTIKTDIKQEDSNTHVYKDGKVIVIGIQLYKDREKMYYFAYEIKDGKAEINREID
                     PIKYMKDHKADYEDENVEVEKD"
     gene            2483..2881
                     /locus_tag="NBH32_001299"
     CDS             2483..2881
                     /locus_tag="NBH32_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1514913.1"
                     /translation="MEKSIKIMTIIGIVVQGLATVFSLLFMVLAASGVMTTDVSTTVN
                     GEVDPVDAETAAAIFTVLFLFLFIFGIISIILGAIGMFKASKNKKMSGILLIIGAVIS
                     GNIITFALWLISGIKLLTNNKPKDEISDLS"
     gene            complement(3130..3954)
                     /locus_tag="NBH32_001300"
     CDS             complement(3130..3954)
                     /locus_tag="NBH32_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HCD1514914.1"
                     /translation="MKEKHIKWDKIFYGDDWVNNVVETIRTKDILQSVYLKRYLLRAM
                     MAGFIIGIITVFVLSVKATHEPDLPPGIVNMASAITFSFALVLILFTNSELLTSNFMY
                     FTVGLYYKVIKPTRVLKIFLLCFAGNILGAAILFSFMRFSNVMTPDMLNQLSAVIEHK
                     TLSTGFVSILMKAIFANFFINISLVIAMQIDDVLAKMFVMMFGVTIFAFMGYEHVVYN
                     SCLFMGGLIYQVDTLHFIPAISNIAAAFIGNYIGGGLIIGLFYAYLNDHHQFYKNN"
     gene            complement(4193..5500)
                     /gene="brnQ"
                     /locus_tag="NBH32_001301"
     CDS             complement(4193..5500)
                     /gene="brnQ"
                     /locus_tag="NBH32_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1514915.1"
                     /translation="MKKQVIISGLMLFSLFFGAGNLIFPPMLGHTAGQNMWIGMLGFA
                     LTGILLPFITVIVVAFYDEGVESVGNRIHPWFGFIFAVVIYMSIGAFYGIPRAANVAY
                     EIGTRHILPVHNQWTLIIFAAIFFAIVYWISLNPSKIVDNLGKLLTPLLLLMVALLSI
                     AVIFNPESALSAPKDKYITHPFISGSLEGYFTMDLVAALAFSVVIVNGYKFKGLTDRM
                     KILKYVCFSGLIAAILLGMIYFALAYVGASTAPGNFKDGTDILTYNSLRVFGSFGNIV
                     FGMTVILACLTTCIGLVNACATFTKKHVPKFSYKIFALIFSIIGFLFTTLGLEMILKI
                     AVPLLTLIYPVSIALVLISFANMFSTFRFSWAYRLATVITLIISILQILNSFNLLHGV
                     ILKWFMMLPLADIDLAWLVPFMLFAIIGFIIDVVIRRPKQATT"
     gene            6084..6974
                     /locus_tag="NBH32_001302"
     CDS             6084..6974
                     /locus_tag="NBH32_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HCD1514916.1"
                     /translation="MNKISKYIAIASLSVAVTVSAPQTTNSTAFAKSSAEVQQTQQAS
                     IPASQKVNLGNQNIMAVAWYQNSAEAKALYLQGYNSAKTQLDKEIKKNKGKHKLAIAL
                     DLDETVLDNSPYQGYASIHNKPFPEGWHEWVQAAKAKPVYGAKEFLKYADKKGVDIYY
                     ISDRDKEKDLKATQKNLKQQGIPQAKKSHILLKGKDDKSKESRRQMVQKDHKLVMLFG
                     DNLLDFTDPKEATAESREALIEKHKDDFGKKYIIFPNPMYGSWEATIYNNNYKASDKA
                     KDKLRKNAIKQFDPKTGEVK"
     gene            7224..8273
                     /locus_tag="NBH32_001303"
     CDS             7224..8273
                     /locus_tag="NBH32_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1514917.1"
                     /translation="MFLAWNEIRRNKLKFGLIIGVLTMISYLLFLLSGLANGLINMNK
                     EGIDKWQADAIVLNKDANQTVQQSVFNKKDIENKYKKQATLKQTGEIVSNGHQKDNVL
                     VFGVEKSSFLVPSLIEGHKATKDNEVLADETLKNKGFKIGDTLSLSQSDEKLHIVGFT
                     ESAKYNASPVIFTNDATIAKINPRLTGDKINAVVVRDTNWKDKKLNQELEAVSINDFI
                     ENLPGYKPQNLTLNFMISFLFVISATVIGIFLYVMTLQKTSLFGILKAQGFTNGYLAN
                     VVISQTLILALFGTAFGLLLTGVTGAFLPDAVPVKFDVLTLLVFAIVLMIVSVLGSLF
                     SILTIRKIDPLKAIG"
     gene            8286..8963
                     /locus_tag="NBH32_001304"
     CDS             8286..8963
                     /locus_tag="NBH32_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1514918.1"
                     /translation="MLKFENVTKSFKDGNRNIEAVKDTNFEINKGDIIALVGPSGSGK
                     STFLTMAGALQTPTSGHILINNQDITTMKQKALAKVRMSEIGFILQATNLVPFLTVKQ
                     QFTLLKKKNKNVMSNEDYQQLMSQLGLTSLLNKLPSEISGGQKQRVAIAKALYTNPSI
                     ILADEPTAALDTENAIEVIKILRDQAKKRKKACIIVTHDERLKAYCDRSYHMKDGVLN
                     LENETVE"
     gene            9171..10202
                     /locus_tag="NBH32_001305"
     CDS             9171..10202
                     /locus_tag="NBH32_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HCD1514919.1"
                     /translation="MDLLIGTLFLFLVLVIFTLFTYKAPNGMRAMGALANAAIATFLV
                     EAFNKYVGGEVFGIKFLEELGDAAGGLGGVAAAGLTALAIGVSPVYALVIAAACGGMD
                     LLPGFFAGYMIGYVMKYTEKYVPDGVDLIGSIVILAPLARLIAVLLTPVVNSTLIRIG
                     DIIQSSTNTNPIIMGIILGGIITVVGTAPLSSMALTALLGLTGVPMAIGAMAAFSSAF
                     MNGTLFHRLKLGDRKSTIAVSIEPLSQADIVSANPIPIYITNFFGGAIAGLIIAMSGL
                     INDATGTATPIAGFLVMFGFNHPTTIVIYGVVMAIVGALAGYLGSIVFKKYPIVTKQD
                     MINRGAVDA"
     gene            10545..11663
                     /locus_tag="NBH32_001306"
     CDS             10545..11663
                     /locus_tag="NBH32_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1514920.1"
                     /translation="MSDSEKEILKRIKDNPFISQRELAEAIGLSRPSVANIISGLIQK
                     EYVMGKAYVLNEDYPIVCIGAANVDRKFYVHKNLVAETSNPVTSTRSIGGVARNIAEN
                     LGRLGETVAFLSASGQDSEWEMIKRLSTPFMNLDHVQQFENASTGSYTALISKEGDMT
                     YGLADMEVFDYITPEFLIKRSHLLKKAKCIIVDLNLGKEALNFLCAYTTKHQIKLVIT
                     TVSSPKMKNMPDSLHAIDWIITNKDETETYLNLKIESTDDLKIAAKRWNDLGVKNVIV
                     TNGVKELIYRSGEEEIIKSVMPSNSVKDVTGAGDSFCAAVVYSWLNGMSTEDILIAGM
                     VNAKKTIETKYTVRQNLDQQQLYHDMEDYKNGKFTKVY"
     gene            11638..12561
                     /locus_tag="NBH32_001307"
     CDS             11638..12561
                     /locus_tag="NBH32_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCD1514921.1"
                     /translation="MANLQKYIEYSREVQQARENNQPIVALESTIISHGMPYPQNVEM
                     ATTVEQIIRNNGAIPATIAIIDGKIKIGLESEDLEILATSKDVAKVSRRDLAEVVAMK
                     CVGATTVATTMICAAMAGIQFFVTGGIGGVHKGAEHTMDISADLEELSKTNVTVICAG
                     AKSILDLPKTMEYLETKGVPVIGYQTNELPAFFTRESGVKLTSSVETPERLADIHLTK
                     QQLNLEGGIVVANPIPYEHALSKAYIEAIINEAVVEAENQGIKGKDATPFLLGKIVEK
                     TNGKSLAANIKLVENNAALGAKIAVAVNKLL"
     gene            12572..13792
                     /locus_tag="NBH32_001308"
     CDS             12572..13792
                     /locus_tag="NBH32_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1514922.1"
                     /translation="MNILFAITGIAFALFVAFLFSFDRKNIDFKKTLIMIFIQVLIVL
                     FMMNTTIGLTILTALGSFFEGLINVSKAGINFVFGDIQNKNGFTFFLNVLLPLVFISV
                     LIGIFNYIKVLPFIIKYVGIAINKITRMGRLESYFAISTAMFGQPEVYLTIKDIIPRL
                     SRAKLYTIATSGMSAVSMAMLGSYMQMIEPKFVVTAVMLNIFSALIIASVINPYKSDD
                     SDVEIDNLTKSTETKSVNGKTGKPKKVAFFQMIGDSAMDGFKIAVVVAVMLLAFISLM
                     EAINIMFGSVGLNFKQLIGYVFAPIAFLIGIPWSEAVPAGSLMATKLITNEFVAMLDF
                     KNVLGDVSARTQGIISVYLVSFANFGTVGIIVGSIKGISDKQGEKVASFAMRLLLGST
                     LASIISGSIIGLVL"
     gene            complement(13897..15429)
                     /locus_tag="NBH32_001309"
     CDS             complement(13897..15429)
                     /locus_tag="NBH32_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HCD1514923.1"
                     /translation="MKEVGFGTLNWVAVIIYLLAMLFIGVYFTKRASQSTNSFFTASG
                     RLPSWVVGFSIYATTLSAITFMSTPEKAFLTDWSYIAGNIAIVAIIPLLIYFYVPFFK
                     KLKVTSAYEYLEARFGPSIRVIGSLLFVVYHLGRVAIVIYLPTLAITSVSDMNPYIVA
                     SLVGLLCILYTFLGGFEGVVWSDFIQGVILLGGALVIIILGVMNIKGGFGTVFADAIE
                     HKKLISADNWKLNTAAAAIPIIFLGNIFNNLYQYTASQDVVQRYQASDSLKETNKSLW
                     TNGILALISAPLFYGMGTMLYSFYTHEAVLPKGFNTSSVVPYFILTEMPPFVAGLLIA
                     AIFAAAQSTISSSLNSISACISIDIKQRFFGKGSERHEVNFARFIIIIAGIFGFGMSL
                     YLIASNSNDLWDLFLFVTGLFGVPLAGVFAVGIFTKRTNTFGVICGLILGIIFAYVYN
                     GVGKGNSPFYVSTISFTVAFVFAYILSFIVPSKHKKDITGLTIFEKDKPSTYISKTAT
                     KK"
     gene            complement(15469..16350)
                     /locus_tag="NBH32_001310"
     CDS             complement(15469..16350)
                     /locus_tag="NBH32_001310"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCD1514924.1"
                     /translation="MNKDLKGLYAALLVPFDENGQVNEQGLKQIAQNAIETEELDGLY
                     VNGSSGENFLLNTEQKKQVFKVAKEAVGDKVKLIAQVGSLDLNEAIELGKYATELGYD
                     ALSAVTPFYYPFTFEEIRDYYFDIIEATQNNMIIYAIPDLTGVNISIEQFSELFNHEK
                     IVGVKYTAPNFFLLERIRKAFPDKLILSGFDEMLVQATISGVDGAIGSTYNVNGRRAR
                     KIFDLARQGQIQEAYQLQHDSNDIIETVLSMGIYPTLKEILRHRGIDAGLPKRPFKPF
                     NEAHRQTLDQLIAKYDL"
     gene            16508..17368
                     /locus_tag="NBH32_001311"
     CDS             16508..17368
                     /locus_tag="NBH32_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCD1514925.1"
                     /translation="MYYIAIDIGGTQIKSAVIDKQLNMFDYQQIPTPDNKSELITDKV
                     YEIVTGYMKQYQLIQPVIGISSAGVVDEQKGEIVYAGPTIPNYKGTNFKRLLKSLSPY
                     VKVKNDVNAALLGELKLHQYQAERIFCMTLGTGIGGAYKNNQSHIDNGELHKANEVGY
                     LLYRPTENTTFEQRAATSALKKRMIDGGFTRSTHVPVLFEAAEEGDDIAKQILNEWAE
                     DVAEGIAQIQVMYDPGLILIGGGISEQGDNLIKYIEPKVAHYLPKDYVYAPIQTTKSK
                     NDAALYGCLQ"
     gene            complement(17645..18445)
                     /locus_tag="NBH32_001312"
     CDS             complement(17645..18445)
                     /locus_tag="NBH32_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCD1514926.1"
                     /translation="MKFENRVQRYQHLFTKTDKRIVNYIRQNGYSDAFSTINSLAHAI
                     GTSPATMTRFSHKLDYENFQDLKFNIQQEMTETVIENSPIIQRIHKYHQQIIQQTGEF
                     IDNDIIQTFIDKLQSSRHILFAGLGSSGLSATEFYYRMIRMGLKGNVTTDSHLMKISA
                     SLLSHSDMFIAMSNSGNTSELISAAEVAKSHGAYVVAITNFEGSKLTDCADLVLLTTD
                     QSRNTDHQFINTQIATLFLIDIVSYHLLENTNLSQTYQHTKSIILDNK"
     gene            18585..19253
                     /locus_tag="NBH32_001313"
     CDS             18585..19253
                     /locus_tag="NBH32_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCD1514927.1"
                     /translation="MLPHGLIVSCQALPDEPLHSSFIMSKMALAAYEGGAVGIRANTK
                     EDILAIKETVDLPVIGIVKRDYDHSDVFITATSKEVDELIESQCEVIALDATLQQRPK
                     ETLDELVSYIRTHAPNVEIMADIATVEEAKNAARLGFDYIGTTLHGYTSYTQGQLLYQ
                     NDFQFLKDVLQSVDAKVIAEGNVITPDMYKRVMDLGVHCSVVGGAITRPKEITKRFVQ
                     VMED"
     gene            complement(19384..20697)
                     /locus_tag="NBH32_001314"
     CDS             complement(19384..20697)
                     /locus_tag="NBH32_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD1514928.1"
                     /translation="MNQYHSNAQQPSAWRFFVYSLVGILCFFIPFTINGNNTIFVDHV
                     HLAIRSIIGPLMPYVALIMILIGTALPIVRRTFMTSITNLVITLFKVAGAMIGIMYVF
                     KIGPSILFKANYGPFLFEKLMMPLSILIPVGAIALSLLVGYGLLEFVGVYMEPIMRPI
                     FKTPGKSAVDAVASFVGRYSLGLLITNRVYKQGMYNKREATIIATGFSTVSATFMIIV
                     AKTLGLMPHWNLYFWITLVITFVVTAITAWLPPITNESTEYYNGQEGEQEVAIEGSRL
                     KTAYAEAMKQNALTPSLVKNVWDNLKDGLEMTVGILPSILSIGFLGLIVANYTPFIDW
                     LGYIFYPFIYIFPIADQALLAKASAISIVEMFLPSLLVTKAAMSTKFVVGVVSVSAII
                     FFSALVPCILATEIKIPVWKLIIIWFLRVALSLLITIPVALLIFG"
BASE COUNT         7418 a         3061 c         3861 g         6483 t
ORIGIN      
        1 ggtttagaaa gcggatctaa attttaataa tgattaatga aaggtagatg aacatgagta
       61 actcaaacgg tttgttaaat ttgtcccttc atttatttgc gttgaacatc acgaggctgt
      121 ttccattttc gaaaaatgga aacgagtatt gtaagtaaaa actctgaaga atctaaggag
      181 attatatagt ttcaagatga tgaaccaatt taggatgaaa atttaaatgg atggatatat
      241 ttagatctta ctgaattgta aaaatagaat ttcaagttat ttgaatgtat ttataaatat
      301 atcgttatta ttaaagtaga gattaggggc gataacatga ctttcgaaga aaaattaagt
      361 gaaatgtaca atgaaattgc gaatgagatt agcagtatga taccagtaga gtgggaaaag
      421 gtatatgcaa ttgcctatgt agatgatcaa ggtggagagg tcgtttttaa ttatacaaaa
      481 ccaggtagtg atgaattgaa ttattacacg aatatatcta gaggttataa tgtttcggaa
      541 gaaatatttg atgatttatg gatgaatctg tattacttgt ttaagaattt aaggaattta
      601 tttaaagaag aaggactcga accatggaca tcatgcgaat ttgactttac aagagacggc
      661 aaattgaaag tatcatttga ttatatagat tggattaata cagagtttga tcaattgggc
      721 cgtgaaaatt actatatgta caaaaagttt ggtgttttac cagaaatgga atacgagatg
      781 gaagaaatta aagaaatcga gcaatatatt aaagagcaag atgaagctga actatagggg
      841 agataatatg actttcgaag aaaaactaag tcaaatgtac aacgagattg cgaatgagat
      901 tagcagcatg ataccagtag agtgggaaaa ggtatatgta attgcctatg tagatgatgg
      961 aggtggagag gtcattttta attatactaa accaggtagc gatgaattga attattacac
     1021 atatatccct agagagtata atgtctctga aaaagtattt tatgatttgt ggacggattt
     1081 atatagattg tttaagaagt taagaaatgc atttaaagaa gaaggacttg aaccatggac
     1141 atcatgtgaa tttgactttg caagagatgg caaattaaat gtatcttttg attatattga
     1201 ttgggtaaat acagagtttg atcaattggg ccgtgaaaac tattatatgt ataaaaagtt
     1261 tggtgtttta ccagaaacgg aatatgaaat ggaagaaatt cgagcagtag agaagtatgt
     1321 taaagagcaa gagtagcaga catgttataa aaggctgtgc aaaaccaacc ttgttttaca
     1381 tttgactcaa agaagaaggt aaaagataag attatttgca acttaaaaag tcaattagtt
     1441 tatcggtatc catacatcat ggataaatga gttcaactaa ttaacaaatt acgatataaa
     1501 tttaaaattg tcattaattg aaaaggttat tgtagtgtat tttataaagt tgatgtacat
     1561 cgacttattt tttaaaactt atatcaaaaa aatatgagaa aagtatatac tattaaaatg
     1621 ttttttacta taattataaa tagttaaata tttggttatg aaataaatgt gtgggaggca
     1681 atttttagag atacatagca atgtaaaggt atcaaatagt tattgcataa tttattatga
     1741 tgacataatt ttcagtgaat agttgtaaat taacactgaa atagatgttt atattaagaa
     1801 gactttaata tcataattaa tcaaaatctt gcgtcaaaca tttaaacgaa ttttaagttg
     1861 ataacaattg gttaaacatt gttcaaacat cacaatgata aagcatatta tcagtattgt
     1921 agtgtgtgga aaatgacagc catctaagga gaaaaatgat gaaaagaata ttggtagtat
     1981 ttttaatgtt agcaattgta ttagcaggtt gttctaataa aggagaaaag tatcaaaaag
     2041 atattgataa agtgtacaaa aaacagaatc aaatgaataa aattgcctcg aaagtgcaaa
     2101 acactattaa aacagacatt aaacaagaag acagtaatac acatgtttat aaagatggta
     2161 aagtcattgt tattggtatt caattatata aagatcgtga aaaaatgtat tatttcgcat
     2221 atgaaataaa agatggtaag gcagagatta atagagaaat agacccaatt aagtatatga
     2281 aagaccataa agcagattat gaagatgaaa atgtagaagt ggaaaaagat taacgtaggc
     2341 tttctttaaa acttttaacc agtttcaaaa agtaaatgag ctttagcgac aataattttt
     2401 aaaataaata cgatataaat ttaaacattt atatataaga acacaaacgt aagtttgtga
     2461 ataataataa aggagtaaga caatggaaaa atcgatcaaa ataatgacaa taataggaat
     2521 tgttgttcaa ggtttagcaa cggtatttag tttactattt atggttttag cagcatcagg
     2581 tgtaatgact acagatgtgt caacaacagt taatggtgag gttgacccag ttgatgcaga
     2641 aacagcagca gcaattttca ctgtattatt cttattccta tttatatttg gaatcatttc
     2701 aattatttta ggtgcaatcg gtatgtttaa agcatctaaa aacaaaaaaa tgagtggtat
     2761 attgttgatt attggagctg taataagtgg taacataatt acatttgctt tatggttaat
     2821 cagtggtatt aaactactta ctaataacaa gcctaaagat gaaataagcg acttatcata
     2881 aacatcgtat attgaaattt caagatttct taagtaatta tatttctgtt agtttttaac
     2941 aaattaaccg attaaataaa gtgctctctt agaagtagat tttcagtcaa taactgcttt
     3001 taagagagca ttttgtattt gataatgctc atagcatcgg acaaaaagca ttgacgaact
     3061 taaaaaaata cgttagacaa tgcacttcta aatattaaag gcatcgacta acgcattgtt
     3121 caaatatagt taattatttt tataaaattg atgatgatca ttcaagtaag catagaataa
     3181 acctataatg agtccgcctc caatatagtt accgataaaa gccgcagcga tattcgaaat
     3241 agctggtatg aagtgcaatg tatcaacttg ataaattaaa ccacccataa ataagcaact
     3301 gttgtaaacg acatgttcat aacccataaa ggcgaatatg gtaacaccga acatcatgac
     3361 aaacattttt gcaagtacat cgtcaatttg catggcgata actaatgaaa tattaataaa
     3421 gaagttggcg aatatcgctt tcattaatat acttacaaac ccagtagaca acgttttatg
     3481 ttctataact gctgataatt gatttaacat atctggcgtc attacatttg aaaaacgcat
     3541 gaaactaaat aaaatagcag cacctaaaat atttcctgca aagcacaata aaaatatttt
     3601 caatactcta gttggtttaa ttactttata atacaggcct acagtaaagt acatgaagtt
     3661 actggttagt agttcggagt ttgtaaataa aatgagtact aacgcaaagc tgaatgtaat
     3721 ggcactggcc atattcacaa tgcctggcgg taaatctggt tcgtgtgttg ctttaactga
     3781 taatacgaag accgtaataa ttccgataat aaatcctgcc atcatcgcgc gtaataaata
     3841 acgttttaaa taaacgcttt gtaatatatc ttttgttctt attgtttcga ctacgttatt
     3901 tacccagtca tccccataaa atattttatc ccatttaata tgtttctcct tcacaatgac
     3961 atcctcactt ttcatttgtt aatcagagta tactatgaat cactttattt gtgaataatt
     4021 tcacaaaatt aattttaaaa actaatattt ttgaataaca gctaggattt tttaacaaat
     4081 catttatttt aaaataatta atcgtcagtt agtatcaatg aataaataca gattttcatg
     4141 caaaaaaacc tagtaatgat gcgacgatca ttactaggag attatgcgtc tattaagttg
     4201 tcgcttgttt cggacggcgt ataactacat cgatgatgaa accgataata gcaaagagca
     4261 tgaatggtac aagccaagct aaatcgatat ctgctaaagg taacatcata aaccatttca
     4321 aaataacacc gtgtaataag ttgaaactat ttagtatttg taaaattgaa ataatcaatg
     4381 taataacagt tgcgagtcga taggcccaac tgaatctgaa tgtgctaaac atgttagcaa
     4441 atgatatcag tacaagtgca atagacacgg gatatattaa agtcaataat ggtacagcaa
     4501 tttttaaaat catttctaaa ccaagtgttg taaataagaa ccctatgata gagaaaataa
     4561 gtgcaaatat tttataagaa aacttaggta cgtgtttctt agtaaatgtg gcgcaagcat
     4621 tgacgagtcc tatacatgtt gttaaacatg caagtatcac tgtcatacca aatactatgt
     4681 tgccaaatga accaaatact cgtaatgagt tgtacgtcaa tatatctgta ccatctttaa
     4741 agtttcctgg agctgttgat gccccaacgt atgcaagtgc aaagtaaatc attccaagta
     4801 atatggctgc aataagacct gaaaagcaga catattttaa aattttcatg cgatctgtga
     4861 ggcctttaaa cttatagcca ttgacaatga ctacggaaaa agctaacgca gcaacaagat
     4921 ccattgtaaa atagccttcc aaacttcctg aaatgaaagg atgtgttata tatttatcct
     4981 taggtgcact tagtgcagat tcagggttga aaatgacagc aatacttaat agagcgacca
     5041 ttaatagtaa taacggtgtt aataatttac ctaaattatc aacgattttc gatggattta
     5101 aactaatcca gtaaacgatg gcaaaaaaga ttgctgcgaa tataattaaa gtccattggt
     5161 tgtgcacagg taaaatgtgt cttgtaccaa tttcgtacgc gacatttgca gcacgtggaa
     5221 taccgtaaaa tgctccgata gacatgtaaa tcacgacagc aaaaataaac ccgaaccatg
     5281 gatgtatacg attgcctaca ctttcaacac cttcatcata aaatgcaaca acaataacag
     5341 taataaaggg gagtaatatg cctgtaagag caaagcctag cataccaatc cacatatttt
     5401 gacccgctgt atggccaagc atgggcggga atattaaatt tccggctcca aaaaatagtg
     5461 aaaataacat gaggcccgaa ataataactt gttttttcaa tgtaatttcc ttcctaaaac
     5521 tagatattca taataattta aaaaatctga aaaataaaaa cgttcttact ttatctttaa
     5581 atgacaatac tatgattcta ttattttata aaaaagattg caataataac gtcttgctat
     5641 tattggataa tgacaattaa agtgtcgttt ttatgaatta aaataggtga aagtaagttg
     5701 gaaatacgtt gaagcgttgt gatttttatg agaaattgta tgagtgagga ctgctattta
     5761 taaaaggtaa actatggatg agtttatggt tgttccaata ggtaaataag agatagcaca
     5821 ctgtacacat aatgatacgt ggatgagtga atatgtctga taggaaggcc tagtcactca
     5881 aatgaattta agtttattac gtgataaatc acaaatctct ctcatgtgat aggtctccca
     5941 ttaaatcatg cttatatgaa atgttcacat atttgttagc ttttcaagaa ataatattaa
     6001 atatcttttc atagtatagc aatattaaat aaatgcattt ataattttaa ctgtatttta
     6061 aatattaatc atgaggtgat aagatgaata aaatttcaaa gtatattgca atagcatcat
     6121 tatcggtagc ggttacagtt tcagcgccac aaacgacaaa ttctacagcg tttgccaaaa
     6181 gttctgctga agttcaacaa acgcaacaag cttctatacc agcatcgcaa aaggtgaatc
     6241 ttggtaatca aaatattatg gcagtggctt ggtatcaaaa ttcagctgaa gcaaaagcat
     6301 tatatttaca aggttataac agtgcaaaga ctcagttaga taaagagatt aaaaagaata
     6361 aaggtaaaca taagttagct attgctttgg atttagatga aacagtttta gataattctc
     6421 catatcaagg ctatgcatca atacataata aacctttccc agaaggttgg catgaatggg
     6481 tacaagctgc taaagctaaa cctgtctatg gcgcaaaaga attcttgaaa tatgctgaca
     6541 aaaaaggtgt cgatatctac tatatttctg atagagataa agaaaaagat ttaaaggcaa
     6601 cacaaaagaa cttaaaacaa caaggtatcc ctcaagctaa gaagagtcat attttactaa
     6661 aaggtaaaga tgataagagt aaagaatcac gcagacaaat ggttcaaaag gatcataaac
     6721 ttgtcatgct atttggagat aatttattag actttacaga cccaaaagaa gctacagctg
     6781 aatctcgtga agcattaatt gaaaaacata aagacgattt cggtaagaaa tatatcattt
     6841 tccctaaccc aatgtatggt agttgggaag ctacaattta caacaataac tataaagcaa
     6901 gtgacaaagc aaaagataaa ttacgtaaaa atgctattaa gcaattcgat cctaaaacag
     6961 gcgaagttaa ataatatatg aattggacgt ctacatgtac ttaaaggtat atgtaggcgt
     7021 ttttaaattg atccttaaat tttaaatgta ccgtacaaaa ataatagaca attaattgca
     7081 ttttccataa atttgctttt aatataaaaa gttcgggtat aatgaaggat aagtgtatgt
     7141 ataacattac agagcgcaac acgacgtacg tgcatctaag aatataaaca atgtttaaat
     7201 catagtagta gggagagaaa tgcatgtttt tagcttggaa tgaaatacgg cgcaacaaat
     7261 tgaagtttgg actaattatt ggtgtgttaa cgatgattag ttacttgcta tttttattat
     7321 ctggattggc gaatggtctt atcaatatga ataaagaagg cattgataag tggcaagcag
     7381 atgccattgt tctaaataaa gatgccaatc aaactgtgca acaatctgtt tttaacaaga
     7441 aagatattga aaataaatac aagaagcaag ctactttgaa gcaaacaggg gaaattgtgt
     7501 ctaatggcca tcaaaaagac aatgttttag tgttcggtgt tgaaaagtca tcatttttag
     7561 ttccgagttt aatagaaggg cataaagcga ctaaagataa tgaagtgtta gctgatgaaa
     7621 cacttaaaaa taaaggattt aaaattggcg acacattatc actatctcaa tcagatgaaa
     7681 aattgcatat cgtaggtttt acagaaagtg caaaatataa tgcgtcacca gtcattttca
     7741 cgaatgacgc taccattgcc aaaatcaatc ctagattgac tggagataaa attaatgcag
     7801 ttgttgtacg tgatacaaat tggaaagaca aaaaattaaa ccaagagctt gaagcggtaa
     7861 gtattaatga ctttattgaa aatttaccag gttataaacc acagaactta acattaaact
     7921 ttatgatttc attcttattt gtcatttcag ctacagttat aggcattttc ttatatgtca
     7981 tgacattaca aaaaacaagt ttatttggca tattaaaggc tcaaggattt acgaatggct
     8041 atttagcgaa cgtagttatt tcgcagacgc tcatattagc attatttggt acggcatttg
     8101 gcttactgtt aacaggcgtt acaggtgcat ttttacctga tgcagtacct gtcaaattcg
     8161 atgtactaac attgctcgta tttgcaattg tgttaatgat tgtctctgta ttaggaagtt
     8221 tattctccat tttaacaatt agaaaaatag atccgttaaa ggcgattggg taggaggtgt
     8281 agcaaatgtt gaaatttgaa aatgtaacaa agtcatttaa agatgggaat cgtaacattg
     8341 aagcggttaa agatacaaat tttgagataa ataaaggtga tattatagca ttggttggac
     8401 cttctggctc tggtaaaagt acatttctaa ctatggcagg tgctttacaa acgccgacat
     8461 ctgggcacat tttaatcaat aaccaagata ttacgacaat gaagcaaaaa gcattggcaa
     8521 aagttagaat gtctgaaata ggttttattt tacaagctac aaaccttgta ccatttttaa
     8581 cggtaaagca acaatttaca ttattgaaaa agaaaaataa gaatgttatg tctaatgaag
     8641 actatcagca acttatgtca caattaggtc taacttcatt gcttaataag ttaccttcag
     8701 aaatttcagg tggtcagaaa caacgtgtgg cgatagccaa agcgttatat acgaatccgt
     8761 cgattatttt agcggatgaa cctaccgcgg cgttagatac tgaaaatgcg attgaagtca
     8821 ttaaaattct acgtgatcaa gccaaaaaaa gaaagaaagc atgtattatt gttacacatg
     8881 atgaacgact taaagcatat tgtgatcgtt catatcatat gaaagatggc gtccttaatc
     8941 ttgaaaatga aacagtagaa tagttttatt aagccggtac atcatgtgcc ggtattttta
     9001 tgtttatgta ttatttgaat aaactttcac attcaattaa taataattat tatcgaaaat
     9061 tagaaatatt ccgtgaaata taatattttt tgtagtaaaa tggcctctaa gtattcaata
     9121 tttaaatatg gggattgaat ataaaattat cgtaatgggg gtcaatggtt atggatttat
     9181 tgataggtac attattttta tttttggtct tagtgatttt tacattattt acatataaag
     9241 cacctaatgg tatgcgtgcc atgggagcat tagctaatgc agcaatcgca acatttttag
     9301 tagaagcatt taataaatat gttggtggcg aagtattcgg tattaaattt ttagaagagc
     9361 taggagacgc tgcgggaggt ctaggtggtg tcgctgccgc tggattaaca gcattagcta
     9421 tcggtgtgtc accagtatat gcattagtta tagcagccgc gtgcggtggt atggatttat
     9481 taccaggttt ctttgcgggt tatatgattg gatatgtgat gaaatataca gagaaatatg
     9541 tgccggatgg tgtcgactta attggatcga ttgtcatctt agcgccatta gctcgtctta
     9601 ttgcagtatt attaacgcca gtagtgaata gtacattgat tcgaattggt gatattatcc
     9661 aaagtagtac gaatacgaat ccaattatca tgggtatcat tttaggtggt attatcacgg
     9721 ttgtcggcac agcgccattg agttcaatgg cattgacagc attattaggt ttaacgggtg
     9781 tacctatggc tattggtgcc atggcagcat ttagttcggc atttatgaat gggacgctat
     9841 tccatcgctt aaaattaggt gatcgtaagt ctacgattgc agtaagtatt gaacctttat
     9901 cacaagcaga tattgtatca gccaatccaa ttccaatcta tattacaaat ttctttggtg
     9961 gtgcgattgc tggtttaatt attgctatgt caggtttaat taacgatgcg acaggtacag
    10021 ctacaccgat tgcaggattt ttagttatgt ttggatttaa tcatccgacg acaattgtga
    10081 tttatggtgt agtaatggcg attgtaggtg cgcttgcagg ttatcttggt tcaattgtat
    10141 ttaaaaaata tccaattgtt actaagcaag acatgattaa tcgaggtgca gtagacgcat
    10201 agcatcatca tattgaatag taaaaacaaa taaaacatag taacgtgatt cagtcgatgt
    10261 aacagtcgat aatgagtcac gtttttttat agaaaaatat aagacataaa aatgtcataa
    10321 tttattgtcg acaaatatca tactgtataa acatttatca ttttctcaag taccttttac
    10381 acgatggaat gaacttactt tttacgaaat tatgcgtatt ttataaacaa atatcattga
    10441 tataacggta aatgtaagcg tttacaacag aaataacagc atgctacgat atttttgtaa
    10501 attcactgat tcaagtattt taagtcaata tgaggaggga tgttatgagc gattctgaga
    10561 aagaaatttt aaaaagaatt aaagataatc cgtttatttc acaacgtgaa cttgctgagg
    10621 caattggatt atctagaccc agcgtagcaa acattatttc aggattaata caaaaggaat
    10681 atgttatggg aaaggcatat gttttaaatg aagattatcc tattgtttgt attggcgcag
    10741 cgaatgtaga tcgtaagttt tatgtgcata aaaatttagt tgcagaaaca tcaaatcctg
    10801 taacgtcaac acgctctatt ggtggcgtag caagaaatat tgctgagaac ttaggtaggc
    10861 ttggcgaaac ggtcgctttt ttatctgcta gtggacaaga tagtgaatgg gaaatgatta
    10921 aacgattgtc cacaccattt atgaatttgg atcatgttca acaatttgaa aatgcgagta
    10981 caggttcata tacagcttta attagtaaag aaggcgacat gacatatggc ttagcagata
    11041 tggaagtgtt tgactacatt acgcctgaat ttttaattaa gcgttcacac ttattgaaaa
    11101 aggctaagtg cattattgta gatttgaatt taggcaaaga ggcattaaac ttcttatgtg
    11161 cctataccac gaaacatcaa atcaaattag ttatcaccac ggtttcttcc ccaaaaatga
    11221 aaaatatgcc tgattcatta catgctattg attggattat cacgaataaa gatgaaacag
    11281 aaacatactt aaatttaaaa atagaatcta ctgatgattt aaaaatagct gctaaacgct
    11341 ggaatgattt aggtgttaaa aatgttattg tgacaaatgg cgtgaaagaa ctcatttatc
    11401 gaagtggtga ggaagaaatc attaagtcag ttatgccatc aaatagtgtg aaagatgtta
    11461 caggtgcagg cgattcattc tgtgctgcag tagtgtatag ctggttaaat gggatgtcta
    11521 ctgaagatat attaattgct ggtatggtta acgcaaagaa aacgatagaa acaaaatata
    11581 cagttaggca aaacctagat caacagcaac tttatcacga tatggaggat tataaaaatg
    11641 gcaaatttac aaaagtatat tgagtattct cgagaagttc agcaagcacg ggagaacaat
    11701 caaccgattg tagcgttaga atcaacaatt atatcgcatg gtatgccgta cccacaaaat
    11761 gttgaaatgg caacaacagt agagcaaatt atcaggaata atggtgccat tccagcaacc
    11821 atagccatta tagatggcaa aattaaaatt ggtttagaaa gcgaagattt agaaatactg
    11881 gcaactagta aagacgttgc taaagtatct agaagggatt tagcagaagt tgttgcgatg
    11941 aagtgtgttg gtgctactac tgtagcgacg acgatgatat gtgctgcaat ggctggtatt
    12001 caattttttg ttacaggagg tattgggggc gtccataaag gtgcagaaca tacgatggac
    12061 atttcagcag acttagaaga actgtctaaa acaaatgtca ctgttatctg tgcaggtgcc
    12121 aaatcaattt tagacttacc taagacgatg gagtatttag aaacaaaagg cgttccagtt
    12181 attggatatc aaacgaatga attgccagca ttcttcactc gcgaaagcgg tgttaagtta
    12241 acaagttcgg ttgaaacgcc agaacgactt gctgacattc atttaacaaa acagcagtta
    12301 aatcttgaag gtggcattgt tgttgctaat ccaattccat atgagcatgc cttatcaaaa
    12361 gcatatattg aagcaatcat aaatgaagct gttgttgaag cggaaaatca aggtattaaa
    12421 ggtaaggacg ccacaccgtt cttgttaggg aaaattgtag aaaaaacgaa tggtaaaagt
    12481 ttagcagcaa atataaaact tgttgaaaac aatgcggcgt tgggtgctaa aattgctgtc
    12541 gctgttaata aattattgta ggtgatgata catgaatatt ttattcgcta tcacagggat
    12601 agcatttgca ctatttgttg cgtttttatt cagttttgat cgtaaaaaca tagacttcaa
    12661 aaagacgtta ataatgatat ttattcaagt gttgattgtg ttatttatga tgaacacaac
    12721 gattggttta acaatcttaa ctgcattagg ctcatttttt gaagggttaa taaatgttag
    12781 taaagcaggt ataaactttg tttttggaga tatacaaaat aaaaatggct ttacgttctt
    12841 tttaaatgtg ttgctgccat tagtgttcat ttctgtatta ataggcatct ttaattatat
    12901 taaggtatta ccatttatta tcaaatatgt aggtatcgct attaataaaa taactagaat
    12961 ggggcgctta gaaagttact ttgctatttc aacagcaatg tttggacaac cagaagtata
    13021 tttaacaata aaagatatta ttccaagatt atctagagcg aaattatata caattgcgac
    13081 gtctggtatg agtgctgtta gtatggcaat gttgggttca tatatgcaga tgattgagcc
    13141 caagttcgta gttacagctg taatgttaaa tatttttagt gcgcttatca tcgccagtgt
    13201 aatcaatccc tataaatctg atgatagtga tgttgaaatt gataacttaa ctaaatcaac
    13261 ggaaacgaaa tcagtgaatg gaaaaacagg aaaacctaag aaagttgcct ttttccaaat
    13321 gattggtgat agtgcgatgg atgggtttaa aatcgctgtt gtagtagccg taatgttgtt
    13381 agcatttatt tcattaatgg aagcaattaa tatcatgttt ggtagtgttg gtttgaactt
    13441 taaacagctt attggctatg tgtttgcacc aatcgcattc ttaataggga ttccatggag
    13501 tgaagcagtt ccagctggct ctttaatggc gaccaaatta attacaaatg agtttgtagc
    13561 aatgcttgat tttaaaaatg tcctgggtga tgtatctgct cgaacacaag gtatcatttc
    13621 agtttactta gtaagcttcg ctaattttgg tacggttggt atcatcgtag gttcaattaa
    13681 aggcattagt gataaacaag gagaaaaagt tgcatccttt gcaatgaggc tgttacttgg
    13741 ttcaactcta gcttcaatca tttcaggatc aattattggc ttagtattgt aaattaatcg
    13801 aagtatctaa attaaattca tggcaaagct aaaccccatc accaagttgg cgcaacagcg
    13861 catcataact tggtgatggg attttatcat aacaatctac tttttcgtag ccgtttttga
    13921 aatgtatgtt gatggtttat ctttttcaaa aattgttaat cccgttatat cttttttatg
    13981 ttttgaaggg acaatgaagc taagtatata agcaaagaca aaagcaactg taaatgaaat
    14041 ggtagataca tagaaaggtg agttaccttt gccaacacca ttatagacat aagcaaagat
    14101 gatacccaat attaatccac aaataacacc gaatgtattc gtacgtttag tgaaaatacc
    14161 aactgcaaat acaccagcta atggaacacc gaataatcca gttacaaaca agaataaatc
    14221 ccataagtca tttgaattag aagcaattaa gtatagtgac attccaaaac cgaaaatacc
    14281 tgcaatgatg ataatgaaac gtgcaaagtt aacttcgtgt cgctcgctac cttttccgaa
    14341 gaagcgttgc ttaatgtcga ttgaaataca agcagatata gaatttaaac tagatgaaat
    14401 ggtagactgt gcagcggcga aaatggctgc aataagtaat cctgctacaa atggtggcat
    14461 ctcagtcaaa atgaaatatg gcactacaga tgatgtattg aagccttttg gtaaaacagc
    14521 ttcatgtgta taaaatgaat acagcattgt acccatacca taaaataagg gtgctgaaat
    14581 taaagctagg ataccatttg tccataacga tttatttgtt tcttttaaac tatcagaagc
    14641 ttgataacgc tgcacgacgt cttgactcgc tgtgtattga tacaagttgt tgaaaatatt
    14701 tcctaggaaa ataattggaa tggcagctgc cgcagtattt agtttccaat tgtctgcact
    14761 aattaatttt ttgtgctcaa tcgcatctgc aaagacagtg ccaaaaccgc ctttaatgtt
    14821 cataacacct agaataatga taactaaagc accacctaat aaaatgacgc cttgaatgaa
    14881 atcactccaa accacacctt cgaatccacc taaaaatgta tataaaatac atagtaaacc
    14941 aacgagtgat gcaacgatat aagggttcat gtctgataca gatgtaatcg ctaatgttgg
    15001 taagtagata acgattgcta cacgccctag atgataaacg acaaataata atgagccaat
    15061 aacacgtatg ctagggccga atctagcttc taaatattca tatgcagatg ttacctttaa
    15121 ctttttaaag aatggaacat agaaataaat gagtaatgga ataattgcta cgatagcgat
    15181 gttacctgca atatatgacc aatctgttaa aaatgctttc tctggtgtag acataaacgt
    15241 aattgcactt aatgtagtag cataaatgga aaaaccaact acccaagatg gcaagcgacc
    15301 acttgcagta aagaaactat tcgtactttg actcgcgcgc ttggtaaaat aaacgccaat
    15361 gaacaacata gctagtagat aaatgataac ggcaacccag tttagtgtgc caaatccaac
    15421 ttctttcatg ggcaacatcc cctttacaat gtattgattc tttgatgtct ataaatcgta
    15481 ttttgcaatg agttgatcta atgtttgtcg atgtgcttcg ttaaaaggtt tgaaaggtct
    15541 tttcggtaat cctgcatcaa tgccacgatg acgtaatatt tctttcaatg ttggataaat
    15601 ccccattgat aacactgttt cgataatgtc gtttgaatca tgttgtagtt ggtaagcttc
    15661 ttgaatttga ccttgtcttg ctaagtcgaa gatttttctt gcacggcgac cattaacgtt
    15721 atatgtagaa ccaattgcac catctacgcc agaaatcgta gcttgaacta acatttcatc
    15781 aaagccagat aagattaatt tgtctgggaa tgcttttcta atacgttcga gtaggaagaa
    15841 gttcggcgct gtatatttaa caccaacaat tttttcgtga ttaaatagct cgctgaattg
    15901 ttcaatagaa atattcacac ctgttaaatc tggtattgca taaataatca tattgttctg
    15961 tgttgcttcg ataatatcga aatagtaatc tctaatttct tcaaaagtaa atggatagta
    16021 gaatggtgtt acggcagaaa gtgcatcata accgagttct gtggcatatt tcccaagttc
    16081 aatggcttca tttaaatcta acgaacctac ttgagcaatc aatttcactt tatccccaac
    16141 tgcctctttg gcaactttga aaacttgctt cttctgctct gtatttaata aaaagttttc
    16201 gcctgagcta ccatttacat aaagaccgtc taattcttca gtttcaatgg cattttgagc
    16261 aatttgttta agtccttgtt catttacttg accattttca tcaaaaggaa cgagtaacgc
    16321 tgcatataaa ccttttaaat ctttgttcat tatgaagtcc ctccaaaaat catttgataa
    16381 tatagtttac agttataatt gtaaacgcta tcataaaatg taacaatatc tttttgaaaa
    16441 ttgaagtcat atttatgtat aattaatgaa aatatttttc aaaatcaata gaaatggagt
    16501 gagtaaggtg tattacatcg caatcgatat tggaggcact caaattaaat cggcggttat
    16561 tgataagcaa ttaaatatgt ttgactatca acaaatacca acgccggaca acaaaagtga
    16621 gcttattact gacaaagtat atgagattgt aacaggatat atgaagcaat atcagttgat
    16681 ccaacctgtc ataggtattt catcagcagg cgttgttgat gaacaaaaag gcgaaattgt
    16741 atacgcaggg ccaaccattc cgaattataa aggtactaat tttaagcgat tattaaaatc
    16801 actgtctcct tatgtcaaag taaaaaatga tgtaaacgct gcattactag gcgaattgaa
    16861 attacatcaa tatcaagcag aacggatctt ttgtatgacg cttggtacag gcattggggg
    16921 tgcgtacaaa aataatcaaa gtcatattga taatggtgaa cttcataagg caaatgaagt
    16981 tgggtattta ttgtatcgtc caactgaaaa tacaacgttt gagcaacgtg ctgcaacgag
    17041 tgcattgaaa aagcgcatga ttgacggagg atttacgaga agcacacatg tgccagtgtt
    17101 gtttgaagca gctgaagaag gtgatgatat tgcaaaacaa atattgaatg agtgggcaga
    17161 agatgtagca gaagggattg cccaaataca agtcatgtat gatccagggc ttatattaat
    17221 tggtggcggt atatctgaac aaggagacaa tctcattaaa tatatcgagc cgaaagttgc
    17281 acactattta ccaaaagact atgtttatgc accaatacaa acgactaaga gtaaaaatga
    17341 tgcagcatta tatggctgtt tgcaatgatg attgaaagaa ggagtcattc taaaatagaa
    17401 tttgaaaccg ttacgagaga tgagagctgt tgttagttcc acacatcaca ctctatctag
    17461 gaccaatcta aactatatca accaacagtg tgccacgggc atattaaatt gaagacgctt
    17521 gaacgtatag atctcaaaaa tgtaaaaaaa tatttggtat tgaaattaaa aaagcaccta
    17581 gcaactcgtt gggacaatca cgatgattat ctacagttgc aggtggattt gaatatacaa
    17641 ctagttattt gttgtctagg ataatagatt tagtatgttg ataagtttga ctcagattcg
    17701 tattttctaa taaatgataa cttacgatat cgattaaaaa gagtgtcgca atttgtgtgt
    17761 tgataaattg atggtcggta ttacgcgatt gatccgttgt taaaagtact aaatctgcac
    17821 aatctgtaag tttactacct tcgaaatttg tgatggcaac gacatatgca ccatgagatt
    17881 tggcgacttc cgctgcagaa attaattccg aagtattacc actatttgac atagcaataa
    17941 acatatccga atgagatagt agggatgccg atattttcat taaatgtgaa tcggtagtaa
    18001 cattaccttt tagccccata cgaatcatac gataataaaa ttcagtcgct gataaaccag
    18061 agctacctag tccagcaaag agtatatgtc gacttgattg aagtttgtcg ataaaggttt
    18121 ggataatgtc gttatcaata aattcaccag tttgttgaat gatttgttga tgatatttat
    18181 gaattctttg aataattggg ctattttcaa taactgtctc tgtcatttct tgttgaatat
    18241 taaattttaa atcttggaaa ttctcataat ccagcttatg actaaagcgt gtcatcgttg
    18301 ctggtgatgt accaatcgca tgggctaagg agttaatcgt tgaaaaggca tcgctatagc
    18361 cattttgtct tatataattg acgatgcgtt tatcagtttt tgtaaataaa tgttgataac
    18421 gttgaacacg attctcaaat ttcattgtgt caccccttca tcttaatgat tactattata
    18481 tatgaaaaat attttcaaga tagtaaaaag cattgataaa aattatctta atgatatatt
    18541 gtaaatgact ttacgtgaaa aaacgactta tggagtgagg aataatgtta ccacatggat
    18601 taatagtatc ttgtcaggca ctaccagatg aaccattgca ttcatctttt attatgtcga
    18661 aaatggcatt agctgcgtat gaaggtggtg ctgttggtat tcgcgcaaat actaaggaag
    18721 acattttagc aattaaagaa acggtagatt taccagttat tggcattgtg aaacgtgact
    18781 atgatcactc agatgttttc attactgcaa cgtcaaaaga agttgatgaa ctgatagaaa
    18841 gccaatgtga agtcattgca ttggatgcaa cgttacagca acgtccgaaa gaaacgttag
    18901 acgaattagt atcatatatt agaacacatg caccgaacgt tgaaatcatg gctgatatcg
    18961 cgaccgttga agaagctaaa aatgccgcac gacttggctt tgattatatt ggcacgacgt
    19021 tacatggcta tactagttat acgcaaggac aattacttta tcaaaatgac ttccaatttc
    19081 taaaagatgt actacaaagt gttgatgcaa aagttattgc ggaaggtaat gtcattacac
    19141 cggatatgta taaacgcgtg atggacttag gcgttcattg ttcagtcgtt ggtggtgcga
    19201 taacacgacc aaaagaaatt acgaaacgtt ttgttcaagt tatggaagat taaatgataa
    19261 cgataaaaaa acgagatgac catcattaat taaaggtacc taattatctt aggtggctga
    19321 atgaatgtaa tgggttcatc tcgttttgtt tgtttacgat agtgatttta ttttcaattt
    19381 gaattatcca aaaataagta aagcgacggg gatggtgatt aatagcgaca atgccacgcg
    19441 taaaaaccaa atgatgatga gtttccagac aggtatttta atttcagttg ctagtataca
    19501 tggcactaat gctgagaaaa agataatggc tgatacgctt actacaccga cgacaaattt
    19561 agtactcatt gcagctttag ttactaacaa agatggtaga aacatctcta caatagaaat
    19621 cgctgacgct tttgcaagta aagcctgatc agcaattggg aaaatataaa taaatggata
    19681 gaagatatag ccaagccaat caatgaatgg tgtatagttc gctacaatca gtcctaaaaa
    19741 accaatcgat aatatagaag gcaaaatacc aacagtcatt tctaaaccgt ctttcaaatt
    19801 gtcccaaacg ttcttcacga gggatggtgt taatgcattt tgtttcatcg cctctgcata
    19861 tgcagttttc agtctgcttc cttcaatagc aacttcttgt tctccttctt gtccgttata
    19921 atattctgtt gattcattgg tgattggcgg tagccatgca gtaattgcag tcacgacaaa
    19981 tgtgatgact aaagttatcc aaaagtataa attccaatgc ggcattaatc ctaaagtttt
    20041 agcaacgata atcataaaag ttgctgaaac tgttgaaaag ccagtcgcaa taatcgtggc
    20101 ttctcgtttg ttgtacattc cttgcttata gacacgatta gtaatcaata atcctaagga
    20161 ataacggccg acaaacgaag ctactgcatc gacagcggat tttcctggtg ttttaaaaat
    20221 aggtctcata ataggctcca tataaacacc gacaaattct aataagccat agcctactaa
    20281 taaagaaagc gcaattgcac ctactggaat taagatactt aatggcatca ttaatttttc
    20341 aaacaagaac ggaccatagt tagctttaaa tagtattgat ggaccgattt taaatacata
    20401 cattatacca atcattgcac ctgcaacttt aaataatgta atgaccaagt ttgtgattga
    20461 agtcataaaa gtacgtctca ctattggtaa cgctgtacca attaaaatca taatcagtgc
    20521 aacatagggc ataagtggac ctatgattga gcgaatggct agatgaacat gatcgacgaa
    20581 aatagtgttg ttaccattaa tcgtaaaagg aataaagaaa catagtatgc ccactaaact
    20641 atagacaaaa aaacgccatg cacttggttg ttgtgcatta gaatgatatt gattcattaa
    20701 agcaacccct ttgtttaaat gaatacacaa aactgtatga tgcatcttcc ccttgatgag
    20761 atgaatcatt attttaattt agaaaaatct gaaaacttac tataattgta tagtttgaat
    20821 tat
//
LOCUS       DAHHOJ010000042        20565 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.042, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000042 DAHHOJ010000000
VERSION     DAHHOJ010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20565)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20565)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20565
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.042"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            509..1804
                     /gene="purB"
                     /locus_tag="NBH32_001315"
     CDS             509..1804
                     /gene="purB"
                     /locus_tag="NBH32_001315"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCD1514929.1"
                     /translation="MIERYSREEMSDIWTDQNRYEAWLEVEILACEAWSELGHIPKAD
                     VQKIRQNAKVNVERAQEIEQETRHDVVAFTRQVSETLGEERKWVHYGLTSTDVVDTAL
                     SFVIKQANDIIEKDLERFIDVLAEKAKNYKYTLMMGRTHGVHAEPTTFGVKMALWYTE
                     MQRNLQRFKQVREEIEVGKMSGAVGTFANIPPEIESYVCKHLGIGTAPVSTQTLQRDR
                     HAYYIATLALIATSLEKFAVEIRNLQKTETREVEEAFAKGQKGSSAMPHKRNPIGSEN
                     ITGISRVIRGYITTAYENVPLWHERDISHSSAERIMLPDVTIALDYALNRFTNIVDRL
                     TVFEDNMRNNIDKTFGLIFSQRVLLALINKGMVREEAYDKVQPKAMISWETKTPFREL
                     IEQDESITSVLTKEELDECFDPKHHLNQVDTIFKRAGLA"
     gene            1913..2215
                     /locus_tag="NBH32_001316"
     CDS             1913..2215
                     /locus_tag="NBH32_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514930.1"
                     /translation="MQIEKLRGAALDELFDAILTLENREECYQFFDDLCTVNEIQSLS
                     QRLQVAKMIKQGYTYATIEQESGASTATISRVKRSLQWGNDAYTMILDRMNIETNE"
     gene            2387..3079
                     /locus_tag="NBH32_001317"
     CDS             2387..3079
                     /locus_tag="NBH32_001317"
                     /EC_number="2.5.1.n9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenylglyceryl phosphate synthase"
                     /protein_id="HCD1514931.1"
                     /translation="MYDIKKWRHIFKLDPAKHISDDDLDAICMSQTDAIMIGGTDDVT
                     EDNVIHLMSRVRRYPLPLVLEISNIESVMPGFDFYFVPTVLNSTDVAFHNGILLEALK
                     TYGHSIDFEEVIFEGYVVCNADSKVAKHTKANTDLTTEDLEAYAQMVNHMYRLPVMYI
                     EYSGIYGDVSKVQAVSEHLTETQLFYGGGISSEQQATEMAAIADTIIVGDIIYKDIKK
                     ALKTVKIKESSK"
     gene            3076..5268
                     /gene="pcrA"
                     /locus_tag="NBH32_001318"
     CDS             3076..5268
                     /gene="pcrA"
                     /locus_tag="NBH32_001318"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HCD1514932.1"
                     /translation="MNALLNHMNTEQSEAVKTTEGPLLIMAGAGSGKTRVLTHRIAYL
                     LDEKDVSPYNVLAITFTNKAAREMKERVQKLVGDQAEVIWMSTFHSMCVRILRRDADR
                     IGIERNFTIIDPTDQKSVIKDVLKNENIDSKKFEPRMFIGAISNLKNELKTPADAQKE
                     ATDYHSQMVATVYSGYQRQLSRNEALDFDDLIMTTINLFERVPEVLEYYQNKFQYIHV
                     DEYQDTNKAQYTLVKLLASKFKNLCVVGDSDQSIYGWRGADIQNILSFEKDYPEANTI
                     FLEQNYRSTKTILNAANEVIKNNSERKPKGLWTANTNGEKIHYYEAMTERDEAEFVIR
                     EIMKHQRNGKKYQDMAILYRTNAQSRVLEETFMKSNMPYTMVGGQKFYDRKEIKDLLS
                     YLRIIANSNDDISLQRIINVPKRGVGPSSVEKVQNYALQNNISMFDALGEADFIGLSK
                     KVTQECLNFYELIQSLIKEQEFLEIHEIVDEVLQKSGYREMLERENTLESRSRLENID
                     EFMSVPKDYEENTPLEEQSLINFLTDLSLVADIDEADTENGVTLMTMHSAKGLEFPIV
                     FIMGMEESLFPHIRAIKSEDDHEMQEERRICYVAITRAEEVLYITHATSRMLFGRPQS
                     NMPSRFLKEIPESLLENHSSGKRQTIQPKAKPFAKRGFSQRTTSTKKQVSSSDWNVGD
                     KVMHKAWGEGMVSNVNEKNGSIELDIIFKSQGPKRLLAQFAPIEKKED"
     gene            5272..7275
                     /gene="ligA"
                     /locus_tag="NBH32_001319"
     CDS             5272..7275
                     /gene="ligA"
                     /locus_tag="NBH32_001319"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCD1514933.1"
                     /translation="MADLSSRVNELHDLLNQYSYEYYVEDNPSVPDSEYDKLLHELIK
                     IEEEHPEYKTVDSPTVRVGGEAQASFNKVNHDTPMLSLGNAFNEDDLRKFDQRIREQI
                     GNVEYMCELKIDGLAVSLKYVDGYFVQGLTRGDGTTGEDITENLKTIHAIPLKMKEPL
                     NVEVRGEAYMPRRSFLRLNEEKEKNDEQLFANPRNAAAGSLRQLDSKLTAKRKLSVFI
                     YSVNDFTDFNARSQSEALDELDKLGFTTNKNRARVNNIDGVLEYIEKWTSQRESLPYD
                     IDGIVIKVNDLDQQDEMGFTQKSPRWAIAYKFPAEEVVTKLLDIELSIGRTGVVTPTA
                     ILEPVKVAGTTVSRASLHNEDLIHDRDIRIGDSVVVKKAGDIIPEVVRSIPERRPEDA
                     VTYHMPTHCPSCGHELVRIEGEVALRCINPKCQAQLVEGLIHFVSRQAMNIDGLGTKI
                     IQQLYQSELIKDVADIFYLTEEDLLPLDRMGQKKVDNLLAAIQQAKDNSLENLLFGLG
                     IRHLGVKASQVLAEKYETIDRLLTVTEAELVEIHDIGDKVAQSVVTYLENEDIRALIQ
                     KLKDKHVNMIYKGIKTSDIEGHPEFSGKTIVLTGKLHQMTRNEASKWLASQGAKVTSS
                     VTKNTDVVIAGEDAGSKLTKAQSLGIEIWTEQQFVDKQNELNS"
     gene            7288..8487
                     /locus_tag="NBH32_001320"
     CDS             7288..8487
                     /locus_tag="NBH32_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CamS family sex pheromone protein"
                     /protein_id="HCD1514934.1"
                     /translation="MKRTLVLLITAIFILAACGNHKDDQAGKDNQKHNNSSNQVKEIA
                     TDKNVQGDNYRTLLPFKESQARGLLQDNMANSYNGGDFEDGLLNLSKEVFPTDKYLYQ
                     DGQFLDKKTINAYLNPKYTKREIDKMSEKDKKDKKANENLGLNPSHEGETDPEKIAEK
                     SPAYLSNILEQDFYGGGDTKDKNIKGMTIGLAMNSVYYYKKEKDGPTFSKKLDDSEVK
                     KQGKQMASEILSRLRENDDLKDIPIHFAIYKQSSEDSITPGEFITQATAEKSQTKLNE
                     WHNINEKSALLPSSTAADYDENLNNNFKQFNDNLQSYFSNFTQAVGKVKFVDKKPQRL
                     VVDLPIDYYGQAETIGITQYVTEQANKYFDKIDNYEIRIKDGNQPRALISKTKDDKEP
                     QVHIYSN"
     gene            complement(8576..10114)
                     /gene="putP"
                     /locus_tag="NBH32_001321"
     CDS             complement(8576..10114)
                     /gene="putP"
                     /locus_tag="NBH32_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proline symporter PutP"
                     /protein_id="HCD1514935.1"
                     /translation="MLTMGTALSQQVDANWQTYIMIAVYFLILIVIGFYGYKQATGNL
                     SEYMLGGRSIGPYITALSAGASDMSGWMIMGLPGSVYSTGLSAMWITIGLTLGAYINY
                     FVVAPRLRVYTELAGDAITLPDFFKNRLNDKNNVLKIISGLIIVIFFTLYTHSGFVSG
                     GKLFESAFGLDYHFGLILVAFIVIFYTFFGGYLAVSITDFFQGVIMLIAMVMVPIVAM
                     MNLNGWGTFHDVAAMKPTNLNLFKGLSFIGIISLFSWGLGYFGQPHIIVRFMSIKSHK
                     MLPKARRLGISWMAVGLLGAVAVGLTGIAFVPAYHIKLEDPETLFIVMSQVLFHPLVG
                     GFLLAAILAAIMSTISSQLLVTSSSLTEDFYKLIRGEEKAKTHQKEFVMIGRLSVLAV
                     AIVAIAIAWNPNDTILNLVGNAWAGFGASFSPLVLFALYWKGLTRAGAVSGMVSGALV
                     VIVWIAWIKPLAHINEIFGLYEIIPGFIVSVIVTYVVSKLTKKPGAFVETDLNKVRDI
                     VREK"
     gene            10480..10782
                     /gene="gatC"
                     /locus_tag="NBH32_001322"
     CDS             10480..10782
                     /gene="gatC"
                     /locus_tag="NBH32_001322"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HCD1514936.1"
                     /translation="MTKVTREEVEHIANLARLQISPEETEEMANTLESILDFAKQNDS
                     ADTEGVEPTYHVLDLQNVLREDKAIKGIPQELALKNAKETEDGQFKVPTIMNEEDA"
     gene            10784..12241
                     /gene="gatA"
                     /locus_tag="NBH32_001323"
     CDS             10784..12241
                     /gene="gatA"
                     /locus_tag="NBH32_001323"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HCD1514937.1"
                     /translation="MSIRYESVENLLTLIKDKKIKPSDVVKDIYDAIEETDPTIKSFL
                     ALDKENAIKKAQELDELQAKDQMDGKLFGIPMGIKDNIITNGLETTCASKMLEGFVPI
                     YESTVMEKLHNENAVLIGKLNMDEFAMGGSTETSYFKKTVNPFDHKAVPGGSSGGSAA
                     AVAAGLVPFSLGSDTGGSIRQPAAYCGVVGMKPTYGRVSRFGLVAFASSLDQIGPLTR
                     NVKDNAIVLEAISGADANDSTSAPVDDVDFTSEIGKDIKGLKVALPKEYLGEGVADDV
                     KEAVQNAVETLKSLGAVVEEVSLPNTKFGIPSYYVIASSEASSNLSRFDGIRYGYHSK
                     EAHSLEELYKMSRSEGFGKEVKRRIFLGTFALSSGYYDAYYKKSQKVRTLIKNDFDKV
                     FENYDVVVGPTAPTTAFNLGEEIDDPLTMYANDLLTTPVNLAGLPGISVPCGQSNGRP
                     IGLQFIGKPFDEKTLYRVAYQYETQYNLHDVYEKL"
     gene            12254..13681
                     /gene="gatB"
                     /locus_tag="NBH32_001324"
     CDS             12254..13681
                     /gene="gatB"
                     /locus_tag="NBH32_001324"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HCD1514938.1"
                     /translation="MHFETVIGLEVHVELKTDSKMFSPSPAHFGAEPNSNTNVIDLAY
                     PGVLPVVNKRAVDWAMRAAMALNMEIATESKFDRKNYFYPDNPKAYQISQFDQPIGEN
                     GYIDIEVDGETKRIGITRLHMEEDAGKSTHKGEYSLVDLNRQGTPLIEIVSEPDIRSP
                     KEAYAYLEKLRSIIQYTGVSDVKMEEGSLRCDANISLRPYGQEKFGTKAELKNLNSFN
                     YVRKGLEYEEKRQEEELLNGGEIGQETRRFDESTGKTILMRVKEGSDDYRYFPEPDIV
                     PLYIDDAWKERVRQTIPELPDERKAKYVNELGLPAYDAHVLTLTKEMSDFFESTIEHG
                     ADVKLTSNWLMGGVNEYLNKNQVELLDTKLTPENLAGMIKLIEDGTMSSKIAKKVFPE
                     LAAKGGNAKQIMEDNGLVQISDEATLLKFVNEALDNNEQSVEDYKNGKGKAMGFLVGQ
                     IMKASKGQANPQLVNQLLKQELDKR"
     gene            13721..13867
                     /locus_tag="NBH32_001325"
     CDS             13721..13867
                     /locus_tag="NBH32_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514939.1"
                     /translation="MIADLDAIEGLFISIEVILLLTLFIVHVNGKNINFINALALIIL
                     RQTV"
     gene            14560..15507
                     /locus_tag="NBH32_001326"
     CDS             14560..15507
                     /locus_tag="NBH32_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1514940.1"
                     /translation="MRKRARIIYNPTSGKELFKRELPDALIKLEKAGYETSAYATEKI
                     GDATLEAERAMHENYDVLIAAGGDGTLNEVVNGIAEKPNRPKLGVIPMGTVNDFGRAL
                     HIPNDIMGALDVIIEGHSTKVDIGKMNNRYFINLAAGGQLTQVSYETPSKLKSIVGPF
                     AYYIKGFEMLPQMKAVDLRIEYDGNVFQGEALLFFLGLTNSMAGFEKLVPDAKLDDGY
                     FTLIIVEKSNLAELGHIMTLASRGEHTKHPKVIYEKAKAINISSFTDLQLNVDGEYGG
                     KLPANFLNLERHIDVFAPNDIVNEELINNDHVDDNLIEE"
     gene            15588..16949
                     /gene="rlmD"
                     /locus_tag="NBH32_001327"
     CDS             15588..16949
                     /gene="rlmD"
                     /locus_tag="NBH32_001327"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCD1514941.1"
                     /translation="MQAIAKNDIKMGTVVDLTHEGHGVVKIDRFPIFIPQALINEQIE
                     YKIIKVKKNFAIGKLLNINTRSENRVAPPCIYYERCGGCQLQHLSYEAQLEMKKEQVI
                     NLFQRKAHFDNSKFNDTVGMTDPWRYRNKSQIPVGKNEQNEVIMGFYRQRSHDIIDME
                     SCLIQDSQHQEVMNEVKSILKDLNVSIYQEQLKKGLMRHLVVRTGYHTDEMMIIFVTN
                     GKKWPQKNAVVEKILDAFPNVTSIKQNINDSHSNVIMGRQSITLYGKDTIIDQLTDST
                     FKISDQSFYQINSEQTEKLYNKAIEYAQLTGNEVVLDTYCGIGTIGLYMAPHAKHVYG
                     VEVVPSAIEDAQQNATINQCNNTTFVCGKAEEVILQWKAQGIKPDVVMVDPPRKGCDE
                     TFIQTLLTLEPKRIVYISCNPATQQRDALLLAEKYQLEEVTPVDMFPQTTHVETVALF
                     NLK"
     gene            17119..17649
                     /locus_tag="NBH32_001328"
     CDS             17119..17649
                     /locus_tag="NBH32_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD1514942.1"
                     /translation="MHKIDLTTNNFQMRRFIILQLVIALFVILFTYKWALGVTAVVDQ
                     NIIINLVYGFAGFIILLILHELIHRALFLLFKKDSKPMFNIKKDRILFQTADACFNKW
                     QFSIIMLSPLFLLSTGLLILIKVFGYSSLIFMFSMHTAYCFIDILLVALTISSSFKYV
                     QQDEDSIYLYHQKPTQ"
     gene            17896..18966
                     /gene="dinB"
                     /locus_tag="NBH32_001329"
     CDS             17896..18966
                     /gene="dinB"
                     /locus_tag="NBH32_001329"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011302771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCD1514943.1"
                     /translation="MTERRIIHIDMDYFFAQVEMRDNPKLKGKPVIVGGKASSRGVVS
                     TASYEARKYGVHSAMPMSQAHKLCPNGYFVTSNFGAYRETSAQIMSIFRSYTDKVEPM
                     SLDEAYLDITELVRPDLPASKIAQYIRKDILEQTHLTASAGVSYNKFLAKLASGMNKP
                     DGLTVIDYQNVHDILMTLDIGDFPGVGKASKKVMHDNGIFNGRDLYEKTEFELIRLFG
                     KRGRGLYNKARGIDHSEVKSSRVRKSVGTERTFATDVNDDEEILRKVWELSGKTAERL
                     NKLQKSAKTVTVKIKTYQFETLSKQMSLRDSVSSEEDIYNIAYLLYNDLKDPDVPIRL
                     IGVTVGNLEQSTYKNMTIYDFI"
     gene            complement(19034..19588)
                     /locus_tag="NBH32_001330"
     CDS             complement(19034..19588)
                     /locus_tag="NBH32_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HCD1514944.1"
                     /translation="MIQDAFVALDFETANGKRTSICSVGMVKVIDSQITETFHTLVNP
                     QDYFSQQNIKVHGIQPEDVENAPTFDYVFPYMMQFIADLPVVAHNAAFDMNVLHQSIQ
                     NIGLPTPNLTYFCSYQLAKRTVDSYRYGLKHMMEFYQLDFHGHHDALNDAKACAMITF
                     RLLKNYENLTYVTNIYGKNLKDKG"
     gene            19804..19896
                     /locus_tag="NBH32_001331"
     CDS             19804..19896
                     /locus_tag="NBH32_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514945.1"
                     /translation="MTKNTIISLENEKTQINDSENESSDLRKAK"
     gene            complement(19978..20478)
                     /gene="ftnA"
                     /locus_tag="NBH32_001332"
     CDS             complement(19978..20478)
                     /gene="ftnA"
                     /locus_tag="NBH32_001332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H-type ferritin FtnA"
                     /protein_id="HCD1514946.1"
                     /translation="MLSKNLLEALNDQMNHEYFAAHAYMAMAAYCDKESYEGFANFFI
                     QQAKEERFHGQKIYNYINDRGAHAEFRAVSAPKIDFSSILETFKDSLSQEQEVTRRFY
                     NLSEIARQDKDYATISFLNWFLDEQVEEESMFETHINYLTRIGDDSNALYLYEKELGA
                     RTFDEE"
BASE COUNT         7592 a         2965 c         3797 g         6211 t
ORIGIN      
        1 caatagaaca tatattcttt ttgtgagtag aaaatttgtt aaattagaag cgaataattt
       61 taaaaggcat tatggaatgt aagaaggaat attatgaaac taggtaaagt agattgttga
      121 aaattccata caataggaga agtaaagtga ataaaatctg gtattactaa agctattgat
      181 tgtcctaatt gtaataaaaa gttggatagt gtagttgagg ggagaaaaat taaaatgcaa
      241 tattatattc ttaatggatc attataaata ccagttagat taaagtgttg cgaacattta
      301 caattagaaa tcagtattaa tctgccatag ataattgtat taatgtaaaa aataaaatag
      361 aatgagaata tacggaaatt ttttatacat ttataaccaa ttctgttaaa ctgtgaagag
      421 tagagttaaa aacgaacttt ttatttattt ttattaaaaa tgttctgttt ttgaaaattc
      481 gagtatacat acgataggag ttaaaaaaat gattgaacgt tattctagag aagaaatgtc
      541 tgatatttgg actgatcaaa atcgctatga agcatggtta gaagtggaaa ttttagcatg
      601 tgaggcatgg agtgaattag gacatatacc gaaagctgac gtacaaaaaa ttcgtcaaaa
      661 cgcaaaggta aatgtcgaac gtgcacaaga aattgaacaa gaaacgcgcc atgatgttgt
      721 agcctttact agacaagttt ctgaaacgct aggtgaagaa cgtaagtggg tacattatgg
      781 tttaacttct actgatgttg tagatacagc tttaagtttc gttattaaac aagcaaatga
      841 cattattgaa aaagatttag aaagatttat cgacgtttta gctgaaaaag caaaaaatta
      901 taaatataca ttgatgatgg gacgtacaca tggagtgcat gcagaaccaa ctacatttgg
      961 tgtcaaaatg gcattgtggt acacggaaat gcaacgtaac ttacaacgtt tcaaacaagt
     1021 aagagaagaa attgaagtag ggaaaatgag tggtgcagta ggtacttttg ctaacattcc
     1081 tcctgaaata gaaagttatg tgtgtaaaca tttaggtatt ggcacagcgc ctgtatcaac
     1141 gcaaacattg caacgcgatc gacatgcata ctacattgcg acattagcat taatcgcgac
     1201 gtcactagaa aaatttgccg ttgaaattcg taaccttcaa aaaaccgaaa caagagaagt
     1261 tgaagaagca tttgcaaaag gccaaaaagg ttcatctgca atgcctcata aacgaaatcc
     1321 aattggctct gaaaatatca caggtatttc aagagtcatt cgtggttata ttactacagc
     1381 ttatgagaat gtgccattat ggcatgaaag agatatttct cattcttcag ctgagcgtat
     1441 tatgttacca gatgttacaa tagcactaga ttatgcattg aatcgtttca cgaatattgt
     1501 ggaccgttta actgtatttg aagataatat gcgtaataac atagataaaa catttggtct
     1561 gattttctca caacgtgtct tattagcatt aattaataaa ggtatggttc gtgaagaagc
     1621 atacgacaaa gtacaaccaa aagcgatgat atcttgggaa acgaaaacac ctttccgtga
     1681 attaatcgaa caagatgagt ctattacaag tgttttaaca aaagaagaat tggatgaatg
     1741 tttcgatcct aaacatcatt tgaatcaagt tgacactatt ttcaaacgcg ctggattagc
     1801 ataaaattaa ataaagtaac gattcagatt taataaaata taaatcgcta cactttaata
     1861 ggttaatgcg ttaaaatgac ataaagaata aaattatagg ggttgtttcg atatgcaaat
     1921 tgaaaaatta cgaggtgcag cgttagatga attgtttgat gcaatactaa cgttagaaaa
     1981 tagagaagaa tgttaccaat ttttcgatga tttgtgtact gtaaatgaaa ttcaatcact
     2041 gtctcaaaga ttacaagttg ctaaaatgat taagcaaggt tatacctatg caacgattga
     2101 acaagaatct ggagcatcga ctgcaacgat ttctagagtg aagcgttcat tacaatgggg
     2161 taatgatgct tatacaatga ttttagatcg tatgaatatt gaaacaaatg aataataaat
     2221 gaacgataaa caatggttat ctatctgcac taataaggta gataatcatt gttttttcac
     2281 gaaaaaattt acagagtaaa agaacttaaa attcatatta agtctttaga actcgacact
     2341 taaaaatgct ataatcatat gtatgttaaa aaaggagttt cggaaaatgt atgacattaa
     2401 aaaatggcgc catattttta aattagaccc agctaaacat atttcagatg atgatttaga
     2461 tgcgatttgt atgtctcaaa cagatgcaat tatgattggt ggaactgatg acgttactga
     2521 agataatgtc attcatttaa tgagcagggt aagaagatac ccattgcctt tagtacttga
     2581 aatatcaaac atcgaaagtg taatgcctgg ttttgatttt tattttgtac ctacagtact
     2641 gaacagtaca gatgttgcat ttcacaatgg tatattatta gaagcgctta aaacatatgg
     2701 acatagtata gattttgaag aagtaatatt tgaagggtat gtcgtgtgca atgctgatag
     2761 caaagtggca aaacatacca aagcaaatac agatttaaca acagaagatt tagaagcata
     2821 tgcccaaatg gtcaatcata tgtatcgatt accggttatg tatatagagt atagtggcat
     2881 ttatggcgac gtatcaaagg ttcaagctgt ctcagaacat ctaacagaaa cgcaactttt
     2941 ttatggtggc ggtatttcct cagaacaaca agcgacagag atggcagcta ttgcagatac
     3001 aattatcgtc ggtgatatta tttataaaga tattaaaaaa gctttaaaaa cagtaaaaat
     3061 aaaggagtct agtaaatgaa tgcgttatta aatcatatga atacagagca aagtgaagct
     3121 gtaaagacaa cagaaggacc attgttaatt atggcaggtg ctggttcagg gaagacacgt
     3181 gttttaacac atagaattgc ttatttatta gacgaaaaag atgtctcacc atacaatgtt
     3241 ttggctatta cttttacaaa taaagctgca agagaaatga aagaacgtgt tcaaaaatta
     3301 gtaggtgatc aagcagaagt tatttggatg tcaacattcc actcaatgtg tgttcgtatt
     3361 ttacgtcgtg atgcagatcg aataggtata gaacgcaatt ttacgataat tgatcctaca
     3421 gaccaaaaat ctgttattaa agacgtctta aaaaatgaaa atattgatag taaaaagttt
     3481 gaacctcgta tgtttatcgg tgcgatcagt aatttgaaaa atgaacttaa aacacctgca
     3541 gatgctcaaa aagaagccac agattatcac tcgcaaatgg tagcaacggt ttatagtgga
     3601 tatcaacgcc aattgtcgcg taatgaagcg ttagattttg atgaccttat tatgacaacg
     3661 attaacttat ttgagcgtgt accagaagtt ctagaatatt atcagaacaa attccaatat
     3721 attcatgtag atgagtatca agatactaat aaagcacaat atacattagt taaattatta
     3781 gcaagtaagt ttaaaaactt atgtgttgta ggtgactcag atcagtcaat ttatggttgg
     3841 cgtggtgctg atattcaaaa tatcttatca tttgaaaaag actacccaga agcgaataca
     3901 atctttttag agcaaaatta tcgttcgacg aaaacgattt taaatgcggc taacgaagtg
     3961 attaaaaata attctgaacg taagccaaaa ggactgtgga ctgcaaatac gaatggtgag
     4021 aaaattcatt actatgaagc aatgacagaa cgtgatgaag cggaatttgt aatacgagaa
     4081 attatgaagc atcaacgtaa tggtaagaaa tatcaagata tggcaatttt atatagaaca
     4141 aatgcacaat cacgtgtact tgaggaaaca ttcatgaaat ctaatatgcc atacacaatg
     4201 gttggtggcc aaaagttcta tgaccgtaaa gaaatcaaag atttattaag ttatttgcgt
     4261 ataattgcca atagtaatga tgacattagt ttgcaacgta ttattaatgt tccaaaaaga
     4321 ggtgtaggtc cttcatctgt tgaaaaagtt caaaactatg cacttcaaaa caatatcagt
     4381 atgtttgatg cacttggaga agctgatttt atcggattgt caaaaaaagt gacacaagag
     4441 tgtcttaatt tttacgagtt gatccaaagc ttgataaaag aacaagaatt tttagaaatt
     4501 catgaaatag ttgacgaagt attacaaaag tctggctacc gagaaatgct tgaacgtgaa
     4561 aatacattag aatctcgaag tagattagaa aacatcgatg aatttatgtc agtaccaaaa
     4621 gactatgagg aaaatacccc attagaagaa cagtcattaa ttaacttttt aacggattta
     4681 tcgttagtag ctgatattga tgaggcagat actgaaaatg gcgtaacact aatgacaatg
     4741 cactcggcta agggccttga atttccaatt gtctttataa tggggatgga agaatcttta
     4801 ttcccacata ttagagcgat taagagtgaa gatgatcacg aaatgcaaga agaacgtcgt
     4861 atttgttatg tagcaattac aagggctgaa gaggtgttat atatcactca tgcgacatca
     4921 agaatgttat ttggtcgccc tcagtcaaat atgccatcta gatttttaaa ggaaattcca
     4981 gaatcactat tagaaaatca ttcaagtggc aaacgacaaa cgatacaacc taaggcaaaa
     5041 ccttttgcta aacgcggatt tagtcaacga acaacgtcaa cgaaaaaaca agtatcgtca
     5101 tctgattgga atgtaggtga caaagtgatg cataaagcct ggggagaagg catggtgagt
     5161 aatgtaaacg agaaaaatgg ctcaatcgaa ctagatatta tctttaaatc acaagggcca
     5221 aaacgtttgt tagcgcaatt tgcaccaatt gaaaaaaagg aggattaagg gatggctgat
     5281 ttatcgtctc gtgtgaacga gttacatgat ttattaaatc aatacagtta tgaatactat
     5341 gtagaggata atccatctgt accagatagt gaatatgaca aattacttca tgaactgatt
     5401 aaaatagaag aggagcatcc tgagtataag actgtagatt ctccaacagt tagagttggt
     5461 ggtgaagccc aagcctcttt caataaagtc aaccatgaca cgccaatgtt aagtttaggg
     5521 aatgcattta atgaggatga tttgagaaaa ttcgaccaac gcatacgtga acaaattggc
     5581 aacgttgaat atatgtgcga attaaaaatt gatggcttag cagtatcatt gaaatatgtt
     5641 gatggatact tcgttcaagg tttaacacgt ggtgatggaa caacaggtga agatattacc
     5701 gaaaatttaa aaacaattca tgcgatacct ttgaaaatga aagaaccatt aaatgtagaa
     5761 gttcgtggtg aagcatatat gccgagacgt tcatttttac gattaaatga agaaaaagaa
     5821 aaaaatgatg agcagttatt tgcaaatcca agaaacgctg ctgcgggatc attaagacag
     5881 ttagattcta aattaacggc aaaacgaaag ctaagcgtat ttatatatag tgtcaatgat
     5941 ttcactgatt tcaatgcgcg ttcgcaaagt gaagcattag atgagttaga taaattaggt
     6001 tttacaacga ataaaaatag agcgcgtgta aataatatcg atggtgtttt agagtatatt
     6061 gaaaaatgga caagccaaag agagtcatta ccttatgata ttgatgggat tgttattaag
     6121 gttaatgatt tagatcaaca ggatgagatg ggattcacac aaaaatctcc tagatgggcc
     6181 attgcttata aatttccagc tgaggaagta gtaactaaat tattagatat tgaattaagt
     6241 attggacgaa caggtgtagt cacacctact gctattttag aaccagtaaa agtagctggt
     6301 acaactgtat caagagcatc tttgcacaat gaggatttaa ttcatgacag agatattcga
     6361 attggtgata gtgttgtagt gaaaaaagca ggtgacatca tacctgaagt tgtacgtagt
     6421 attccagaac gtagacctga ggatgctgtc acatatcata tgccaaccca ttgtccaagt
     6481 tgtggacatg aattagtacg tattgaaggc gaagtagcac ttcgttgcat taatccaaaa
     6541 tgtcaagcgc aacttgttga aggattgatt cactttgtat caagacaagc catgaatatt
     6601 gatggtttag gcactaaaat tattcaacag ctttatcaaa gtgaattaat taaagatgtt
     6661 gctgatattt tctatttaac agaagaagat ttattacctt tagacagaat ggggcagaaa
     6721 aaagttgata atttattagc tgccattcaa caagctaagg acaactcttt agaaaattta
     6781 ttatttggtc taggtattag gcatttaggt gttaaagcga gccaagtgtt agcagaaaaa
     6841 tatgaaacga tagatcgatt actaacggta actgaagcgg aattagtaga aattcatgat
     6901 ataggtgata aagtagcaca atctgtagtt acttacttag aaaatgaaga tattcgtgct
     6961 ttaattcaaa aattaaaaga taaacatgtt aatatgattt ataaaggtat caaaacatca
     7021 gatattgaag gacatcctga atttagtggt aaaacgatag tactaactgg taagctacat
     7081 caaatgacac gcaatgaagc atctaaatgg cttgcatcac aaggtgctaa agttacaagt
     7141 agcgttacta aaaatacaga tgtcgttatt gctggtgaag atgcaggttc aaaattaaca
     7201 aaagcacaaa gtttaggtat tgaaatttgg acagagcaac aatttgtaga taagcaaaat
     7261 gaattaaata gttagagggg tatgtcgatg aagcgtacat tagtattatt gattacagct
     7321 atctttatac tcgctgcttg tggtaaccat aaggatgacc aggctggaaa agataatcaa
     7381 aaacataaca atagttcaaa tcaagtaaaa gaaattgcta cggataaaaa tgtacaaggt
     7441 gataactatc gtacattgtt accatttaaa gaaagccagg caagaggact tttacaagat
     7501 aacatggcaa atagttataa tggcggcgac tttgaagatg gtttattgaa cttaagtaaa
     7561 gaagtatttc caacagataa atatttgtat caagatggtc aatttttgga caagaaaaca
     7621 attaatgcct atttaaatcc taagtataca aaacgtgaaa tcgataaaat gtctgaaaaa
     7681 gataaaaaag acaagaaagc gaatgaaaat ttaggactta atccatcaca cgaaggtgaa
     7741 acagatcctg aaaagattgc agaaaaatca ccagcctatt tatctaacat tttagagcaa
     7801 gatttttatg gtggtggaga tacaaaagat aagaatatta aaggtatgac gattggttta
     7861 gctatgaata gtgtttatta ctataaaaaa gaaaaagatg gaccgacttt tagtaaaaaa
     7921 ctagatgata gcgaagttaa aaagcaaggt aaacaaatgg ctagtgagat attatcaagg
     7981 ttacgtgaaa atgatgattt aaaagatata ccaattcatt ttgcaattta taagcaatca
     8041 agtgaagatt caatcacacc aggtgaattt atcactcaag cgactgcaga aaagagtcaa
     8101 acaaagctta atgaatggca taatatcaat gaaaaatcag ctttattacc ttcttcaaca
     8161 gcagcagatt atgatgaaaa tttaaataat aatttcaagc aatttaatga taatttgcaa
     8221 tcatattttt ctaatttcac acaagcagta gggaaagtta aatttgttga taaaaagcca
     8281 caacgattag tagtagattt accaatcgat tactatggac aagctgaaac aattggtatt
     8341 acacagtacg ttactgaaca agcgaataaa tatttcgata aaatcgataa ctatgaaatt
     8401 cgtattaaag atggtaacca accacgagct ttaattagta agacaaaaga tgacaaagaa
     8461 ccgcaagttc acatttatag taattaattg taaattaaag ataataaaaa ggtaccaatg
     8521 ttagtcagaa ttttgattaa cattagtacc tttttaattt gttaagactt atgaattatt
     8581 tttctctaac gatgtcacga actttgttta agtcagtttc aacaaatgca ccaggttttt
     8641 tagtaagttt acttacaaca tatgtaacga ttacacttac aataaatcca ggaataattt
     8701 catataagcc gaatatttcg ttgatatgtg ccaatggttt aatccacgca atccaaacga
     8761 taacgactaa ggcacctgaa accattccac ttacagcacc ggcacgtgtc aaacctttcc
     8821 agtaaagtgc aaatagcaca agtggactga acgatgcacc aaatccggcc caagcattac
     8881 ctactaagtt tagtattgtg tcatttggat tccatgcaat tgcaatagca acgattgcta
     8941 ctgctaatac agataaacgt ccaatcataa caaattcttt ttggtgcgtt ttagcttttt
     9001 cttcaccacg aattaattta taaaagtctt ccgttaatga actagatgtt acaagtaatt
     9061 gtgaagaaat cgtgctcata attgcagcta gaatcgcagc aagtaagaaa ccacctacaa
     9121 gaggatggaa gagtacttga ctcatcacga tgaataatgt ctcaggatct tctagtttaa
     9181 tatgataagc aggtacgaat gcaatacctg ttaaaccaac agccacagcg cctaacaaac
     9241 caactgccat ccagctaata cctaaacgtc tagctttagg tagcatcttg tgtgatttaa
     9301 tagacataaa ccttacaatg atatgaggtt gaccgaaata acctaatccc catgaaaata
     9361 gagagataat tcctataaat gataaccctt taaataaatt taaatttgta ggtttcatag
     9421 ctgctacatc atgaaacgtt ccccagccgt ttaaattcat catagcaaca atagggacca
     9481 taaccatcgc aattaacata atgacacctt ggaagaaatc tgtaattgat acagctaaat
     9541 atccaccaaa gaaagtatag aaaatgacaa tgaaagcaac taatattaaa ccgaaatgat
     9601 aatctaatcc aaaagcactt tcaaataatt taccaccaga tacgaaacca gaatgtgtat
     9661 ataaagtaaa gaatataacg ataattaatc cagaaataat ctttagaaca ttatttttat
     9721 cgtttagtct atttttaaag aaatctggta atgtgattgc atctccagct aattcggtat
     9781 aaacacgaag tctaggagca acaacaaagt aatttatata agcacctaat gttaaaccga
     9841 ttgtaatcca catagctgat agaccagtgc tatagacaga gccaggtaga cccataatca
     9901 tccatccact catatctgaa gctccagctg ataatgcagt aatatacggt ccaatactac
     9961 gtccacctaa catgtactcg cttaggttac cagttgcttg cttgtaaccg taaaagccaa
    10021 taacgattag tatcaaaaag tagacggcaa tcataatata agtttgccaa ttggcatcta
    10081 cttgttgact taatgctgtc cccattgtaa gcatgataaa cactcccttt atttaattta
    10141 taatttagtt acttgaaaac attatacata aagataaatg tttagaaaag taggaaaatt
    10201 aaatttattc tttttgaaat acttgtatat caatatagaa aacgctttcg tattttttgg
    10261 atatgggcaa atttttataa ttaaaattgc ataatcataa tattgaattg ggttgtctaa
    10321 tattaaaggg tgttgttttt ataaaaaatg atttgataaa tgaatgataa tgtttataca
    10381 gtttatatga aattaaagta gtacctcata aatacttaga tttttaattg gaaatttgat
    10441 acaatttagt gatgaatgac ttaaaggagg cttttattaa tgacaaaagt aacacgtgaa
    10501 gaagttgagc atatcgcgaa tcttgcaaga cttcaaattt ctcctgaaga aacggaagaa
    10561 atggccaaca cattagaaag cattttagat tttgcaaaac aaaatgatag cgctgataca
    10621 gaaggcgttg aacctacata tcacgtttta gatttacaaa acgttttacg tgaagataaa
    10681 gcaattaaag gtattccgca agaattagct ttgaaaaatg ccaaagaaac agaagatgga
    10741 caatttaaag tgcctacaat catgaatgag gaggacgcgt aagatgagca ttcgctacga
    10801 atcggttgag aatttattaa ctttaataaa agacaaaaaa atcaaaccat ctgatgttgt
    10861 taaagatata tatgatgcaa ttgaagagac tgatccaaca atcaagtctt ttctagcgct
    10921 ggataaagaa aatgcaatta aaaaagcgca agaattggat gaattacaag caaaagacca
    10981 aatggatggc aaattatttg gtattccaat gggtataaaa gataacatta ttacaaacgg
    11041 attagaaaca acatgtgcaa gtaaaatgtt agaaggtttt gtgccaattt acgaatctac
    11101 tgtaatggaa aaactacata atgagaatgc cgttttaatc ggtaaattaa atatggatga
    11161 gtttgcaatg ggcggttcaa cagaaacatc ttatttcaaa aaaacagtta atccatttga
    11221 ccataaagca gtaccaggtg gttcatcagg tggatctgca gcagcagttg cagctggctt
    11281 agtaccattt agcttaggtt cagacacagg tggttcaatt agacaaccgg ctgcatattg
    11341 tggcgttgtc ggtatgaaac caacatacgg tcgtgtatct cgatttggat tagttgcatt
    11401 tgcatcttca ttagaccaaa ttggtccatt gactcgaaat gtaaaagata atgcaatcgt
    11461 attagaagct atttctggtg cagatgctaa tgactctaca agtgcacctg ttgatgatgt
    11521 agactttaca tctgaaattg gtaaagatat taaaggatta aaagttgcat tacctaaaga
    11581 atacttaggt gaaggtgtag ctgatgacgt aaaagaagca gttcaaaacg ctgtagaaac
    11641 tttaaaatct ttaggtgctg tcgttgagga agtatcattg ccaaatacta aatttggtat
    11701 tccatcatat tacgtgattg catcatcaga agcttcgtca aacctttctc gttttgacgg
    11761 aattcgttat ggttatcatt ctaaagaagc tcattcatta gaagaattat ataaaatgtc
    11821 gagatctgaa ggtttcggta aagaagtaaa acgtcgtatt ttcttaggta catttgcatt
    11881 aagttcaggt tactacgatg cttactataa aaaatctcaa aaagttagaa cattgattaa
    11941 aaatgacttt gataaagtat tcgaaaatta tgatgtagta gttggtccaa cagcgcctac
    12001 aactgcgttt aatttaggtg aagaaattga tgatccatta acaatgtatg ccaatgattt
    12061 attaacaaca ccagtaaact tagctggatt acctggtatt tctgttcctt gtggacaatc
    12121 aaatggccga ccaatcggtt tacagttcat tggtaaacca ttcgatgaaa aaacgttata
    12181 tcgtgtcgct tatcaatatg aaacacaata caatttacat gacgtttatg aaaaattata
    12241 aggagtggaa atcatgcatt ttgaaacagt tataggactt gaagttcacg tagagttaaa
    12301 aacggactca aaaatgtttt ctccatcacc agcgcatttt ggagcagaac ctaactcaaa
    12361 tacaaatgtt atcgacttag catatccagg tgtcttacca gttgttaata agcgtgcagt
    12421 agactgggca atgcgtgctg caatggcact aaatatggaa atcgcaacag aatctaagtt
    12481 tgaccgtaaa aactatttct atccagataa tccaaaggca tatcaaattt ctcaatttga
    12541 tcaaccaatt ggtgaaaatg gatatatcga tatcgaagtc gacggtgaaa caaaacgaat
    12601 cggtattact cgtcttcaca tggaagaaga tgctggtaag tcaacacata aaggtgagta
    12661 ttcattagtt gacttgaacc gtcaaggtac accgctaatt gaaatcgtat ctgaaccaga
    12721 tattcgttca cctaaagaag catatgcata tttagaaaaa ttacgttcaa ttattcaata
    12781 cactggtgta tcagacgtta agatggaaga gggatcttta cgttgtgatg ctaacatctc
    12841 tttgcgtcca tatggtcaag aaaaatttgg tactaaagcc gaattgaaaa acttaaactc
    12901 atttaactat gtacgtaaag gtttagaata tgaagaaaaa cgccaagaag aagaattgtt
    12961 aaatggtgga gaaatcggac aagaaacacg tcgatttgat gaatctacag gtaaaacaat
    13021 tttaatgcgt gttaaagaag gttctgatga ttaccgttac ttcccagagc ctgacattgt
    13081 acctttatat attgatgatg cttggaaaga gcgtgttcgt cagacaattc ctgaattacc
    13141 agatgagcgt aaagctaagt atgtaaatga attaggttta cctgcatacg atgcacacgt
    13201 attaacattg actaaagaaa tgtcagattt ctttgaatca acaattgaac acggtgcaga
    13261 tgttaaatta acatctaact ggttaatggg tggcgtaaac gaatatttaa ataaaaatca
    13321 agtagaatta ttagatacta aattaacacc agaaaattta gcaggtatga ttaaacttat
    13381 cgaagacgga acaatgagca gtaaaattgc gaagaaagtc ttcccagagt tagcagctaa
    13441 aggtggtaat gctaaacaga ttatggaaga taatggctta gttcaaattt ctgatgaagc
    13501 aacacttcta aaatttgtaa atgaagcatt agacaataac gaacaatcag ttgaagatta
    13561 caaaaatggt aaaggcaaag ctatgggctt cttagttggt caaattatga aagcgtctaa
    13621 aggtcaagct aatccacaat tagtaaatca actattaaaa caagaattag ataaaagata
    13681 atttaaatca tcaaactatg aagatttaaa aaatataccc ttgattgctg acttagatgc
    13741 aatcgagggt ttatttatat ctatagaagt catattactt ttaactttat tcattgtaca
    13801 tgttaatggt aaaaatatta attttattaa tgcgttagct ttaattatat taaggcaaac
    13861 tgtataataa aaaggtataa aacatttttg tataaagaca acattatatt tacaacatca
    13921 ttttaaaggt aaaatagcat aactgacgaa gtctatataa cgaagaacgg caaaaaatgc
    13981 tgaataaata acaacctttg tacatattga gatagtattt gtttaagata caagttggtc
    14041 tttaacgata ttaagaatga tgaaataaga ctgagcctgg gtcataaatt caatgtccta
    14101 ggcactacaa tgttatattg gcagtagttg actgaaagaa aatacgcttg taacaagctt
    14161 ttttcaattc tagtggggcc ccaacaaaga gaatttcgaa agaaaattct acagacaatg
    14221 caagttggcg gggccccaac aaagagaatt tcgaaagaaa attctacaga caatgcaagt
    14281 tggcggggcc ccaacaaaga gaatttcgaa aggaaattct acagacaatg caagttgggg
    14341 cggggcccca acatagaagc tgactttctg tcagcttaca ataatgtgca agttggggtg
    14401 gggccctcaa caaagagaat ttcgaaagga aattctacag acaatgtaag ttggggtaga
    14461 acgaaataaa ttttgttaaa tattatttct gtcccactcc ctattagacg aaaccaagat
    14521 gaagttaaaa tatatgaatt ttaagtagag ggataagata tgaggaaacg tgctagaatc
    14581 atttataacc cgacatcagg taaagagcta tttaaaagag aattacctga tgccttaata
    14641 aaattagaaa aagcgggata tgaaacgagt gcatatgcaa ccgagaaaat aggtgatgcc
    14701 acacttgaag cagaaagagc tatgcatgaa aattatgatg tattaatcgc tgcaggtggt
    14761 gacggaacat taaatgaagt agttaatggt atcgcagaaa agcctaatcg tcctaagtta
    14821 ggtgtcattc ctatgggtac tgttaatgac tttggacgtg cattgcatat acctaatgac
    14881 atcatggggg cacttgatgt catcattgaa ggtcattcta ctaaagtaga tattggtaaa
    14941 atgaataatc gatactttat taatttagct gcaggcggac aattgacgca agtctcttat
    15001 gaaacaccga gtaaattgaa atctattgtt ggtccatttg cttattacat caaaggtttc
    15061 gaaatgttac ctcaaatgaa agctgtagat ttaagaattg aatatgatgg taatgttttc
    15121 caaggagaag cattattatt ctttttaggt ttaacaaatt caatggcagg atttgaaaaa
    15181 ttagtgccgg acgctaagtt agatgacggc tattttacgt taattatagt tgaaaaatca
    15241 aaccttgcag aacttggtca tattatgact ttagcttcaa gaggagaaca tactaagcat
    15301 cctaaagtta tttatgaaaa agcaaaggca attaatattt catcattcac agacttacaa
    15361 ctaaatgtag atggagaata tggtggtaaa ttaccagcga atttcttaaa tctagaacgt
    15421 catatagatg tctttgcacc taatgatatt gtaaatgaag agttaataaa taatgatcat
    15481 gttgatgaca atttaatcga agaataaaca gaaatagtga aatatataat ggcaggttag
    15541 gaagcgcata attttgttcc taacctgttc attttggagg taaaattttg caagcaatag
    15601 ctaaaaatga cataaaaatg ggaacagttg ttgatttaac gcatgaaggc catggtgttg
    15661 taaaaataga tcgctttcct attttcatac cacaagcttt aattaatgaa caaattgaat
    15721 ataaaattat caaagtaaaa aagaactttg cgataggtaa gttgcttaat ataaatacga
    15781 gaagtgagaa tcgtgttgca ccgccatgta tatattatga acgttgtggt gggtgccaac
    15841 ttcaacattt atcatacgaa gcacaacttg aaatgaaaaa ggaacaagtg attaatttgt
    15901 ttcagcgaaa agcgcatttt gataattcta agtttaacga tacagttggc atgacagatc
    15961 cttggcgata tcgaaacaag tcccagattc cagttggtaa gaatgaacag aatgaagtta
    16021 ttatgggctt ttatcgtcaa agaagccatg acattattga catggaaagt tgtttaatac
    16081 aagattcaca acatcaagaa gtgatgaatg aagttaaatc tattttaaaa gatttaaatg
    16141 taagtattta tcaagaacaa ttgaaaaaag gtttaatgag acatttggtt gtaagaacag
    16201 gatatcatac agacgaaatg atgattattt ttgtaactaa tggtaaaaag tggccacaaa
    16261 aaaatgctgt tgttgagaag atattagatg catttcctaa tgtgacaagc attaaacaaa
    16321 acattaatga tagccactct aatgttatta tgggacgtca gtctataacg ctatatggta
    16381 aagatacaat tattgatcaa ttaacagata gtacatttaa aataagtgat cagtcattct
    16441 accaaattaa ttctgaacaa acagagaaat tatataataa agcaattgag tatgcgcaat
    16501 tgacaggtaa tgaagttgta ttagatacct attgtggtat tggaacaata gggctatata
    16561 tggcaccaca tgcaaaacat gtatacggtg ttgaagtagt accttctgca attgaagacg
    16621 cacaacaaaa tgcgactata aaccaatgca ataatacaac ttttgtctgt gggaaagctg
    16681 aagaagttat tttacaatgg aaagcacaag ggattaaacc agatgttgta atggttgatc
    16741 cgccaagaaa gggctgtgac gaaacgttta ttcagacttt gcttacatta gaacctaagc
    16801 gtattgtcta tatttcatgt aatcccgcaa cacagcaacg tgatgcatta ttattagctg
    16861 aaaaatatca acttgaagag gtaacaccgg tagatatgtt tccacaaaca acacatgttg
    16921 agacagtggc attattcaat ttgaaatagc gcacatgatt tgagataaaa tttcgcctta
    16981 ataagtgaag aaagaagtcg tacattaaaa ttttattaca gccaatttac tttcttgata
    17041 tgaacttttt aaattaaatg tgacattgta tactatattt aaagaagaat aagaatgtca
    17101 tgatcgggag ggttggtaat gcataaaatt gatttaacga caaataattt tcaaatgcga
    17161 aggtttatta tcttgcaact tgttattgcg ttattcgtga ttttatttac gtataagtgg
    17221 gcgttaggtg tcacagcggt tgtcgatcaa aatatcatca tcaatcttgt atatgggttt
    17281 gctggattca ttattttatt gattttacat gaacttatcc atagagcatt atttctttta
    17341 tttaaaaaag atagcaaacc tatgtttaat ataaaaaaag acagaatctt attccaaaca
    17401 gcagatgctt gcttcaacaa atggcaattc tcaattatta tgctgtcacc actattccta
    17461 ttaagtactg gcttgcttat tttaattaag gtatttggtt attcatcact tatttttatg
    17521 tttagtatgc atactgctta ttgctttata gatattctgc ttgttgcatt aacaatatct
    17581 agtagtttta aatatgttca acaagatgaa gatagtattt atttgtatca tcaaaagccg
    17641 actcaataat aaattttaat atgatgatac caacaaaatg aatttgcttg aattatttca
    17701 gattacctat gttttcttac tcaatcgtct tcatcatcat gcttaaatag caattgattt
    17761 tataacaact tgtagttatc tcaatatagt tatcgctaag tgcaagattt atgtaaaaaa
    17821 atcgtacaca gcgtataatt atgttgagat atttttgtgc gttataaaag taaaaattag
    17881 tagggaggtg agcacttgac tgagagacga attattcata tagatatgga ctattttttt
    17941 gcacaagtcg aaatgagaga taaccccaaa ttgaaaggga aaccagtcat tgttggtggt
    18001 aaagcaagca gtaggggtgt tgtctctacg gcatcctatg aagcacgaaa atacggcgtc
    18061 cattcagcaa tgcctatgtc acaagcgcat aaattatgtc caaatggata ctttgtcaca
    18121 agtaattttg gtgcttaccg agaaacatct gcgcagatta tgtctatttt ccgaagttat
    18181 acagataagg tagaaccgat gtcattggat gaagcgtatt tagatattac agaattagtg
    18241 agaccagacc ttcctgcttc gaaaattgct cagtatatta gaaaagatat tctagagcag
    18301 acacatttaa cagcatctgc aggtgtttct tataacaaat ttttagctaa attagcgagt
    18361 ggtatgaata aacctgatgg tttgactgtg attgattatc aaaatgtcca tgatattttg
    18421 atgacattgg atattggaga ttttccaggc gtaggtaaag cttccaaaaa agtaatgcat
    18481 gataatggta tttttaacgg tagagattta tatgagaaaa cggaatttga attaatacgt
    18541 ttgtttggaa aaagaggtcg gggtttatat aacaaggcac gcggtattga ccatagtgaa
    18601 gtgaaatcat caagagtaag aaaatcagta gggactgaac gcacatttgc aacagacgtg
    18661 aatgatgatg aagagatttt aagaaaagta tgggaattgt caggtaaaac agctgaacgt
    18721 ctaaataaat tacagaagtc agctaaaact gtaacggtta aaattaaaac ttatcaattt
    18781 gaaacgctat ctaaacagat gagtttaaga gattcggtta gttctgaaga agatatttat
    18841 aatattgcat atttacttta taacgattta aaagacccag atgtaccaat tcgacttatt
    18901 ggtgtcactg taggtaattt agaacaatca acttataaaa atatgacgat atatgacttt
    18961 atataaaata aagctccctg caaagtttac atttttacaa tgcttacttt tgaagggagt
    19021 attttattta gtcctagcct ttatctttta gatttttacc ataaatatta gttacatatg
    19081 ttaaattttc ataatttttc agtagcctaa aagtaatcat tgcgcatgct ttggcatcat
    19141 tcaatgcatc atgatgacca tgaaaatcta attgataaaa ctccatcata tgttttaaac
    19201 cgtatcgata cgaatcaacg gttcttttag caagttgata actacaaaag taagttaaat
    19261 ttggagttgg taaaccaata ttttgaatgc tttgatgtaa gacgttcata tcaaatgccg
    19321 cgttatgtgc gacaacaggt aaatctgcaa taaattgcat catatatgga aatacgtagt
    19381 caaacgtagg tgcattttca acatcttctg gttgtatgcc atgaacttta atattttgtt
    19441 gtgaaaaata gtcttgcgga ttcacaagag tatgaaatgt ttctgttatt tgactatcaa
    19501 tgactttaac cattccgaca gaacaaatac ttgtacgttt accatttgct gtttcaaaat
    19561 caagtgcaac aaacgcatct tgtatcatgt agtcaatcct ttcaattaat taaatgtata
    19621 tattaataga cttttagtca tttgcttaca aacgatacat aatattatac gtgaaggtat
    19681 aacgtgaaat ctatcatttg gatgtaactc tgattaaaaa gtaaatgatg atatgcttta
    19741 aaatcggtca aattaattca acatcaaatt tagagttatt aacattagta gtttaaaaaa
    19801 taaatgacta aaaatacaat aattagcctt gaaaatgaaa aaacgcagat caatgattca
    19861 gaaaatgaat ctagtgatct ccgtaaagca aaataatata gacattgggt tattgtaagt
    19921 gacccaaatg cctatcatgt cgtatgatat ttatctgtct attgtagtga tgtttaatta
    19981 ttcttcgtcg aatgtacgag cgccaagttc tttttcgtaa agatataatg cattgctgtc
    20041 atcgccgata cgagttaaat aattgatgtg agtttcaaac attgattctt cttcgacttg
    20101 ttcatctaag aaccagttta agaatgagat agttgcataa tctttatctt gacgagcgat
    20161 ttcagataag ttatagaaac gtcttgttac ttcttgttct tgagataagc tgtctttgaa
    20221 agtttctagt atgcttgaaa agtcaatttt tggtgctgaa actgctctga attctgcatg
    20281 tgcacctctg tcgttaatat agttataaat cttttgtcca tggaaacgtt cttctttagc
    20341 ttgttgaatg aagaagtttg caaatccttc gtacgattct ttatcacagt atgctgccat
    20401 tgccatatat gcgtgtgctg caaagtactc atggttcatt tgatcattta atgcttctaa
    20461 taaattttta cttaacattt tgatacacct cttatttgta aattaaaatt actatgaatt
    20521 aaacttagta taacaaactc attctcaatt gtaaataata ataat
//
LOCUS       DAHHOJ010000043        20210 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.043, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000043 DAHHOJ010000000
VERSION     DAHHOJ010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20210)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20210)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20210
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.043"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            230..721
                     /gene="lspA"
                     /locus_tag="NBH32_001333"
     CDS             230..721
                     /gene="lspA"
                     /locus_tag="NBH32_001333"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCD1514947.1"
                     /translation="MHKKYFIGTSILIAVFVVIFDQVTKYIIATTMKIGDSFEVIPHF
                     LNITSHRNNGAAWGILSGKMTFFFIITIIILIALVYFFIKDAQYNLFMQVAISLLFAG
                     ALGNFIDRILTGEVVDFIDTNIFGYDFPIFNIADSSLTIGVILIIIALLKDTSNKKEK
                     EVK"
     gene            721..1638
                     /locus_tag="NBH32_001334"
     CDS             721..1638
                     /locus_tag="NBH32_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1514948.1"
                     /translation="METYEFNITDKEQTGMRVDKLLPELNSDWSRNQIQDWIKAGLVV
                     ANDKVVKSNYKVKLNDHIVVTEKEVVEADILPENLNLDIYYEDDDVAVVYKPKGMVVH
                     PSPGHYTNTLVNGLMYQIKNLSGINGEIRPGIVHRIDMDTSGLLMVAKNDIAHRGLVE
                     QLMDKSVKRKYIALVHGNIPHDYGTIDAPIGRNKNDRQSMAVVDDGKEAVTHFNVLEH
                     FKDYTLVECQLETGRTHQIRVHMKYIGFPLVGDPKYGPKKTLDIGGQALHAGLIGFEH
                     PVTGEYIERHAELPQDFEDLLDTIRKRDA"
     gene            2038..2565
                     /gene="pyrR"
                     /locus_tag="NBH32_001335"
     CDS             2038..2565
                     /gene="pyrR"
                     /locus_tag="NBH32_001335"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HCD1514949.1"
                     /translation="MSERIIMDDAAIQRTVTRIAHEILEYNKGTDNLILLGIKTRGEY
                     LANRIQDKIHQIEQQRIPTGTIDITYFRDDIEHMSSLTTKDAIDIDTDITDKVVIIID
                     DVLYTGRTVRASLDAILLNARPIKIGLAALVDRGHRELPIRADFVGKNIPTSKEETVS
                     VYLEEMDQRNAVIIK"
     gene            2783..4090
                     /locus_tag="NBH32_001336"
     CDS             2783..4090
                     /locus_tag="NBH32_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCD1514950.1"
                     /translation="MQNDEMFERTVKPVLDVNEKPQPAQWAFLSLQHLFAMFGATVLV
                     PFLTGLPISAALLASGIGTLLYILITKAQIPAYLGSSFAFITPIITGLSTHSLGDMLV
                     ALFMSGVMYVIIGILIKLSGTAWLMKLLPPVVVGPVIMVIGLSLAPTAVNMAMYENPG
                     DMKGYNISFLIVAMITLLVTIVVQGFFKGFLSLIPVLVGIIVGYVVAIFMGIVKFDAI
                     MSAKWIDFPHIYLPFKDYVPSFHLGLVLVMIPIVFVTVSEHIGHQMVLNKIVGRNFFE
                     KPGLDKSIIGDGVSTMFASIIGGPPSTTYGENIGVLAITRIYSIYVIGGAAVIAIVLA
                     FIGKFTALISSIPTPVMGGVSILLFGIIAASGLRMLVESKVDFANNRNLVIASVILVV
                     GIGNLVFNLKEIGINLQIEGMALAALSGIILNLILPKEKKQNN"
     gene            4118..4999
                     /locus_tag="NBH32_001337"
     CDS             4118..4999
                     /locus_tag="NBH32_001337"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HCD1514951.1"
                     /translation="MNHLLSMEHLSTDQIYKLIQKASQFKSGERQLPNFEGKYVANLF
                     FENSTRTKCSFEMAELKLGLKTISFETSTSSVSKGESLYDTCKTLESIGCDLLVIRHP
                     FNNYYEKLANINIPIANAGDGSGQHPTQSLLDLMTIYEEYGYFEGLNVLICGDIKNSR
                     VARSNYHSLKALGANVMFNSPNAWIDDSLEAPYVNIDDVIETVDIVMLLRIQHERHGL
                     AEETRFAADDYHQKHGLNEVRYNKLQEHAIVMHPAPVNRGVEIQSDLVEASKSRIFKQ
                     MENGVYLRMAVIDELLK"
     gene            5017..6291
                     /locus_tag="NBH32_001338"
     CDS             5017..6291
                     /locus_tag="NBH32_001338"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCD1514952.1"
                     /translation="MKLIKNGKVLQNGELQQADILIDGKVIKQIAPAIEPSNGVDIID
                     AKGHFVSPGFVDVHVHLREPGGEYKETIETGTKAAARGGFTTVCPMPNTRPVPDSVEH
                     FEALQKLIDDNAQVRVLPYASITTRQLGKELVDFPALVKEGAFAFTDDGVGVQTASMM
                     YEGMIEAAKVNKAIVAHCEDNSLIYGGAMHEGKRSKELGIPGIPNICESVQIARDVLL
                     AEAAGCHYHVCHVSTKESVRVIRDAKRAGIHVTAEVTPHHLLLTEDDIPGNNAIYKMN
                     PPLRSTEDREALLEGLLDGTIDCIATDHAPHARDEKAQPMEKAPFGIVGSETAFPLLY
                     THFVKNGDWTLQQLVDYLTIKPCETFNLEYGTLKENGYADLTIIDLDSEQEIKGEDFL
                     SKADNTPFIGYKVYGNPILTMVEGEVKFEGDK"
     gene            6293..7393
                     /gene="carA"
                     /locus_tag="NBH32_001339"
     CDS             6293..7393
                     /gene="carA"
                     /locus_tag="NBH32_001339"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCD1514953.1"
                     /translation="MQSKRYLVLEDGSFYEGYRLGSDNLTVGEIVFNTAMTGYQETIS
                     DPSYTGQIITFTYPLIGNYGINRDDFESLVPTLNGIVVKEASAHPSNFRQQKTLHDVL
                     ELHQIPGIAGVDTRSITRKIRQHGVLKAGFTDRKEDIDQLVKHLQQVELPKNEVEIVS
                     TKTPYVSTGKDLSVVLVDFGKKQNIVRELNVRGCNVTVVPYTTTAEEILAMAPDGVML
                     SNGPGNPEVVECAIPMIQGILGKIPFFGICLGHQLFALSQGASSFKMKFGHRGANHPV
                     KNLETGKVDITSQNHGYAIDIDSLKSTDLEVTHLALNDGTVEGLKHKTLPAFSVQYHP
                     EANPGPSDSNYLFDDFVAMMTNFKEKERHINA"
     gene            7386..10559
                     /gene="carB"
                     /locus_tag="NBH32_001340"
     CDS             7386..10559
                     /gene="carB"
                     /locus_tag="NBH32_001340"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCD1514954.1"
                     /translation="MPKRNDIKTILVIGSGPIIIGQAAEFDYAGTQACLALKEEGYRV
                     ILVNSNPATIMTDKEIADKVYIEPLTHDFIARIIRKEQPDALLPTLGGQTGLNMAIQL
                     HESGVLQDNNVQLLGTELTSIQQAEDREMFRTLMNDLNIPVPESDIVNTVEQAFKFKE
                     QVGYPLIVRPAFTMGGTGGGICHNDEELHEIVSNGLHYSPATQCLLEKSIAGFKEIEY
                     EVMRDKNDNAIVVCNMENIDPVGIHTGDSIVVAPSQTLSDVEYQMLRDVSLKVIRALG
                     IEGGCNVQLALDPHSFDYYIIEVNPRVSRSSALASKATGYPIAKLAAKIAVGLTLDEM
                     LNPITGTSYAAFEPTLDYVISKIPRFPFDKFEKGERELGTQMKATGEVMAIGRTYEES
                     LLKAIRSLEYGVHHLGLPNGESFDLDYIKERISHQDDERLFFIGEAIRRGTTLEEIHN
                     MTQIDYFFLHKFQNIIDIEHQLKEHQGDLEYLKYAKDYGFSDKTIAHRFNMTEEEVYQ
                     LRMENDIKPVYKMVDTCAAEFESSTPYYYGTYETENESIVTDKEKILVLGSGPIRIGQ
                     GVEFDYATVHAVWAIQKAGYEAIIVNNNPETVSTDFSISDKLYFEPLTEEDVMNIINL
                     EKPKGVVVQFGGQTAINLADKLAKHGVKILGTSLENLNRAEDRKEFEALLRKINVPQP
                     QGKTATSPEEALANAAEIGYPVVVRPSYVLGGRAMEIVDNDKELENYMTQAVKASPEH
                     PVLVDRYLTGKEIEVDAICDGETVIIPGIMEHIERAGVHSGDSIAVYPPQTLTEDELA
                     TLEDYTIKLAKGLNIIGLINIQFVIAHDGVYVLEVNPRSSRTVPFLSKITDIPMAQLA
                     MRAIIGEKLTDMGYQEGVQPYAEGVFVKAPVFSFNKLKNVDITLGPEMKSTGEVMGKD
                     TTLEKALFKGLTGSGVEVKDHGTVLMTVSDKDKEEVVKLAQRLNEVGYKILATSGTAN
                     KLAEYDIPAEVVGKIGGENDLLTRIQNGDVQIVINTMTKGKEVERDGFQIRRTTVENG
                     IPCLTSLDTANALTNVIESMTFTMRQM"
     gene            10669..11361
                     /gene="pyrF"
                     /locus_tag="NBH32_001341"
     CDS             10669..11361
                     /gene="pyrF"
                     /locus_tag="NBH32_001341"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCD1514955.1"
                     /translation="MKDLPIIALDFESKEKVNQFLDLFDESLFVKVGMELFYQEGPQL
                     INEIKERGHDVFLDLKLHDIPNTVGKAMERLAKLNVDLVNVHAAGGVKMMSEAIKGLR
                     KHNQHTKIIAVTQLTSTTEDMLRHEQNIQTSIEEAVLNYAKLANAAGLDGVVCSPLES
                     RMLTEKLGTSFLKVTPGIRPKGASQDDQHRITTPEEARQLGSTHIVVGRPITQSDNPV
                     ESYHKIKESWLV"
     gene            11361..11972
                     /locus_tag="NBH32_001342"
     CDS             11361..11972
                     /locus_tag="NBH32_001342"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCD1514956.1"
                     /translation="MAKEIAKSLLDIEAVTLSPNDLYTWSSGIKSPIYCDNRVTLGYP
                     LVRGAIRDGLINLIKEHFPEVEVISGTATAGIPHAAFIAEKLKLPMNYVRSSNKSHGK
                     QNQIEGAKSEGKKVVVIEDLISTGGSSVTAVEALKQAGAEVLGVVAIFTYGLKKADDT
                     FSNIQLPFYTLSDYNELIEVAENEGKISSEDIQTLVEWRDNLA"
     gene            12002..12214
                     /locus_tag="NBH32_001343"
     CDS             12002..12214
                     /locus_tag="NBH32_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514957.1"
                     /translation="MNDKTSNDLYGKIKHCNEFINHSNDSNLSSSHDVDESSTKQKHI
                     KNKTTIDHNDDLFKHVKDILRKQGQI"
     gene            12651..13052
                     /locus_tag="NBH32_001344"
     CDS             12651..13052
                     /locus_tag="NBH32_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1514958.1"
                     /translation="MDIPKITTFLMFNNQAEEAVKLYTSLFEDSEIITMAKYGENGPG
                     DPGTVQHSIFTLNGQVFMAIDANSGTELPMNPAISLFVTVKDTIEMERLFNGLKDEGA
                     ILMPKTNMPPYREFAWVQDKFGVSFQLALPE"
     gene            complement(13315..15012)
                     /locus_tag="NBH32_001345"
     CDS             complement(13315..15012)
                     /locus_tag="NBH32_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NFACT family protein"
                     /protein_id="HCD1514959.1"
                     /translation="MAYDGLFTKKMVESLQFLTTGRVHKINQPDNDTILMVVRQNRQN
                     HQLLLSIHPNFSRLQLTTKKYDNPFNPPMFARVFRKHLEGGIIESIKQIGNDRRIEID
                     IKSKDEIGDTIYRTVILEIMGKHSNLILVDENRKIIEGFKHLTPNTNHYRTVMPGFNY
                     EAPPTQHKMNPYDITGAEVLKYIDFNAGNIAKQLLNQFEGFSPLITNEIVNRRQFMTS
                     STLPEAFDEVMAETKLPPTPIFHKNHETGKEDFYFIKLNQFNDDTVTYDSLNDLLDRF
                     YDARGERERVKQRANDLVRFVQQQLHKYQNKLAKLIEEYEQSKNKDTEQLYGELITAN
                     IYRIKQGDKEVTALNYYTNEEVVIPLNPTKSPSANAQYYYKQYNRMKTRERELQHQIQ
                     LTKDNIDYFSTIEQQLHHISVHDIDEIRDELAEQGFMKQRKNQTKKKKAQIQLQHYVS
                     TDGDDIYVGKNNKQNDYLTNKKAKKTHTWLHTKDIPGSHVVIFNDAPSDTTIKEAAML
                     AGYFSKAGNSGQIPVDYTLIKNVHKPSGAKPGFVTYDNQKTLYATPDYELIQKMKQS"
     gene            15287..15910
                     /gene="gmk"
                     /locus_tag="NBH32_001346"
     CDS             15287..15910
                     /gene="gmk"
                     /locus_tag="NBH32_001346"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCD1514960.1"
                     /translation="MDNEKGLLIVLSGPSGVGKGTVRKRIFEDPSTSYKYSISMTTRQ
                     MREGEVDGVDYFFKTRDAFEALIKDDQFIEYAEYVGNYYGTPVQYVKDTMDEGHDVFL
                     EIEVEGAKQVRKKFPDALFIFLAPPSLDHLRERLVGRGTESDEKIQSRINEARKEVEM
                     MNLYDYVVVNDEVELAKNRIQCIVEAEHLKRERVEAKYRKMILEAKK"
     gene            15910..16128
                     /locus_tag="NBH32_001347"
     CDS             15910..16128
                     /locus_tag="NBH32_001347"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCD1514961.1"
                     /translation="MLNPPLNQLTSQIKSKYLIATTAAKRAREIDEQPETELLSEYHS
                     FKPVGRALEEIADGKIRPVISSDYYGKE"
     gene            16344..17543
                     /gene="coaBC"
                     /locus_tag="NBH32_001348"
     CDS             16344..17543
                     /gene="coaBC"
                     /locus_tag="NBH32_001348"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCD1514962.1"
                     /translation="MKKILLAVTGGIAAYKAIDLTSKLTQSGYEVRVMLTNHAQKFVT
                     PLAFQAISRNAVYTDTFIEENPSEIQHIALGDWADAIIVAPATANTIAKLSVGIADDL
                     VTSTLLATETPKFIAPAMNVHMYENKRTQQNINILKEDGYHFIEPGSGFLACGYVAKG
                     RMEEPLQIVSVIDAHFQNSNRLANSSFQDKRALVTAGPTIEVIDPVRFVSNRSSGKMG
                     YAIAEALRNRGAIVTLVAGPTTLEDPKDIEVIHVQSAEEMFEQVTSRFDEQDIVVKAA
                     AVSDYTPVDVLEHKMKKQDGDLSVSFKRTKDILKYLGEHKTSQYLIGFAAETEDIENY
                     AQQKLRKKNADVIISNNVGDMSIGFSSDDNELTMHFKNNEKVNIKKGKKVVLAAQILD
                     ELETRWQ"
     gene            17543..19951
                     /gene="priA"
                     /locus_tag="NBH32_001349"
     CDS             17543..19951
                     /gene="priA"
                     /locus_tag="NBH32_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCD1514963.1"
                     /translation="MIAKVIVDVASKSVDFKFDYIIPEQLESVIQPGVRVIVPFGPRT
                     IQGYVMEVTAEPDAQLDVSKLKKIIEVKDIQPELTSELIALSEWMGSTHVIKRISMLE
                     VMLPSAIKAKYKKAFKMKDDIELPSALLQKFDKHGYYYYKDAQKNNDIQLLMKLLKDD
                     IVEEKTILTQNITKKTKRAVRVIEGYHPDEVLAKLEKVIKQYDLYAYLSEEQHKTIFL
                     TDIEDMGFSKSSLDGLIKKGYVEKYDAVVDRDPFKDRVFEQESKQQLTEDQYKAYEAI
                     KAKIVSQEQETFLLHGVTGSGKTEVYLQTIEDVLSQGKQAMMLVPEIALTPQMVLRFK
                     RRFGDDVAVLHSGLSNGERYDEWQKIRDGRARVSVGARSSVFAPFKNLGLIIIDEEHE
                     STYKQEDYPRYHAREIAQWRSEYHHCPVILGSATPCLESYARAEKGVYHLLSLPNRVN
                     QQALPEIDIVDMREELSEGNRSMFSKDLREAIQLRLDRQEQVVLFLNRRGYASFMLCR
                     DCGYVPQCPNCDISLTYHKTTDLLKCHYCGYQETPPNQCPNCESEHIRQVGTGTQKVE
                     ELLQQEFEDARIIRMDVDTTSKKGAHEKLLTEFEKGNGDILLGTQMIAKGLDYPNITL
                     VGVLNADTMLNLPDFRASERTYQLLTQVAGRAGRHEKAGQVIIQTYNPDHYSILDVQK
                     NDYLTFYRQEMEYRKLGKYPPYYYLINFTISHKEMKKVMEASQHVHKILLQHLTEKAL
                     VLGPSPAALARINNEFRFQILVKYKSEPGLLQAIQFLDDYYHEKFIKEKLALKIDIDP
                     QMMM"
BASE COUNT         7140 a         2937 c         3814 g         6319 t
ORIGIN      
        1 ttttaaattg cctaagaact taatgtccca gcctgtttcc taatttttaa tgtcatctta
       61 aatatgataa atgaataatt aagttcatat ttaatgtcaa aacatagtag tttatcaagt
      121 attgagttag taacattaga tttaatgtaa tatgcttact ttttttatta gcaggtgtaa
      181 gctataatat aaagagttgt ctcatggacg attgattgga ggaacgaaaa tgcacaaaaa
      241 atattttatt ggcacttcca ttttaatagc agtatttgtc gttatatttg accaagttac
      301 taaatatatt atagctacta caatgaaaat tggagattca tttgaagtga taccgcactt
      361 tttaaacata acatcacatc gaaataatgg tgctgcatgg ggaatattga gtggaaaaat
      421 gacatttttc tttattatta ccattattat attaatagcc ttagtatatt tctttattaa
      481 agatgctcaa tataatttgt ttatgcaagt tgctattagt ttactttttg caggtgcact
      541 tggaaacttt attgatagaa ttttaacagg agaagttgtt gactttattg atacaaatat
      601 ttttggttat gattttccaa tatttaatat cgcagattca agtttaacaa ttggtgtaat
      661 attaattatt attgccttat taaaggatac ttccaataaa aaggagaagg aggttaagta
      721 atggagactt atgaatttaa cattacagat aaagaacaaa caggtatgcg tgtagataag
      781 ttgctgcctg aattaaatag tgattggtct cgtaaccaga tacaagattg gattaaagca
      841 ggtttagtcg ttgcaaacga taaagttgtt aaatctaatt ataaagtgaa acttaatgat
      901 catatagttg tcactgaaaa agaagtggtt gaagctgata ttctacctga aaatttaaat
      961 ttagatattt attatgaaga tgacgatgtt gcagttgtat ataaaccgaa aggcatggta
     1021 gttcatccat caccagggca ttataccaat acattagtta atggtttaat gtatcaaatt
     1081 aaaaatttgt caggtattaa tggagaaatt cgtccaggta ttgttcaccg tatagatatg
     1141 gatacttctg gtttattaat ggttgctaaa aatgatattg ctcatcgtgg gcttgtagaa
     1201 caattaatgg ataaatctgt taaaagaaaa tatatcgctt tagttcacgg gaatattcct
     1261 catgattacg gtacaatcga tgcgccaatt ggtagaaaca aaaatgatcg tcaatctatg
     1321 gctgttgttg atgatggtaa ggaagcagtg acacatttta acgtactaga acattttaaa
     1381 gattatacgc ttgttgaatg tcaacttgaa acaggacgta cgcatcaaat ccgtgtgcac
     1441 atgaaatata ttggcttccc attagttggt gatccaaagt atggaccgaa aaagacattg
     1501 gatattggtg gtcaagctct acatgctgga cttattggat tcgaacatcc agtaacaggt
     1561 gaatatattg aaagacatgc tgaattacca caagactttg aagatttatt agatacaatt
     1621 cgaaaaagag atgcataatt gtgttgtgct ataattacga taacattatt atgtaaacta
     1681 taagtattta tcgttttcgt ttttttaaaa tgaaaacaca ttataaaata gattaaggtt
     1741 aacattttta cttagatcgg ttaatttttc aatatagatt gacaaagcgc ctgattagta
     1801 ttatccttat aacagtttaa gtacaacata aagagtcttt aattgagcgt ccagagagac
     1861 gtcaaagaca tgaaaatata gatatatact aaaatgatca taacactttc acatatgcaa
     1921 gaataagtag taatactacg attcttctat gtatataggc tgattgtata tactaaacct
     1981 catgtcgata ttaaagacat gaggtttttt gtttctaaat tttgaaaggg tgagaatatg
     2041 tctgaacgta tcataatgga tgatgccgca atacaacgta cagtgacgag aatcgctcat
     2101 gaaattttgg agtataacaa aggtactgat aatttaattc ttttaggtat caaaacaaga
     2161 ggtgaatatt tagcgaatcg tatacaagat aaaattcatc aaattgagca acaacgtata
     2221 cctactggaa caattgatat tacatacttt agagatgata tagagcacat gtcatcactt
     2281 acgacaaaag acgcaataga catcgacaca gatattacag ataaagtagt catcattatt
     2341 gacgatgtgc tgtatactgg tcgaacggtt cgtgcttcac ttgatgctat tttgctaaat
     2401 gctagaccta ttaaaattgg tttagctgct ttggttgatc gaggacatcg tgagttacca
     2461 attcgagcag attttgttgg taaaaatata cctacttcta aagaggaaac ggtaagtgtc
     2521 tatttagaag aaatggatca aagaaatgca gttataatta aataataccc cttttaattc
     2581 agtacgagag actgaaaaag gtgggggagt tgaatgataa ataagttttc atatgcttgt
     2641 ttatctaata ataccctcat gttcgacaac tataagtcaa tttctatgga cgaaaatgac
     2701 ttgtagcaac ttgtcacact ttttgtctct ttacatatac tgatgtaaag agattttttt
     2761 attatcggag gaaatgaata ttatgcaaaa tgatgaaatg tttgaacgaa cagtaaaacc
     2821 cgtactagat gtaaatgaaa aaccacaacc agcgcaatgg gcatttttaa gcttacaaca
     2881 tttatttgcg atgtttggcg caacagtact agtaccattc ttaacaggac taccaatatc
     2941 agcagcgtta ctagcttcgg gaatcggtac attactttat atcttaataa cgaaggcgca
     3001 aataccagca tacttgggat ctagctttgc atttatcaca ccaattatca cgggattaag
     3061 tacgcatagc ttaggagaca tgcttgtagc attattcatg agtggtgtta tgtacgtcat
     3121 catcggaatt ctaatcaaat taagtgggac agcatggtta atgaaattat taccaccagt
     3181 tgttgttgga ccagtcatca tggtaattgg tttaagctta gcgcctactg cagtcaatat
     3241 ggcaatgtat gaaaatcctg gagatatgaa aggttacaat atcagtttct taattgttgc
     3301 aatgataacc ttgcttgtaa caatagtcgt tcaaggtttt tttaaaggat tcctatcttt
     3361 aattccagtt cttgtaggta ttatcgtagg ttatgtggta gcgattttta tggggattgt
     3421 gaaatttgat gcaattatgt cagcaaaatg gatagatttc cctcatattt atctgccatt
     3481 taaagattat gtaccttcat ttcacttagg acttgtactt gtaatgattc cgattgtgtt
     3541 cgtaacagta agtgaacata ttgggcacca aatggtactg aataaaatcg taggtagaaa
     3601 cttctttgaa aagccaggac ttgataaatc aatcattggt gatggtgttt ctacaatgtt
     3661 tgccagtatt attggtggac caccaagtac aacatacggt gaaaatatcg gtgtattagc
     3721 gattaccaga atatacagta tttacgtcat tggtggtgca gcagttatag caattgtttt
     3781 agcattcatt ggtaagttca ctgcattaat ttcttctata cctacaccag ttatgggagg
     3841 agtatctata ttacttttcg gtattattgc agcaagtggc ttaagaatgt tagttgaaag
     3901 caaagtagat tttgcgaaca atcgaaattt agttatagct tcagtaattt tagttgtagg
     3961 tatcggtaat ttagtattta acttaaaaga aattggtatc aaccttcaaa ttgaggggat
     4021 ggcattagct gcactttcag gaattatttt gaacttaatc ttacctaaag agaaaaaaca
     4081 aaacaattaa gatttacaaa ttaaggaggg cgcttttatg aatcatttat tatcaatgga
     4141 acatttatct acagatcaaa tatacaaact tatccaaaag gcaagtcaat ttaaatctgg
     4201 tgaacgtcaa ctaccaaact ttgaagggaa atatgtcgca aatttattct ttgaaaattc
     4261 tactcgaaca aaatgtagtt ttgaaatggc agaacttaag ctagggttaa aaacgattag
     4321 ctttgaaaca tcaacatcat ctgtttcaaa aggtgaatct ttatatgaca catgtaaaac
     4381 tttagaaagt attggctgtg atttattagt cattagacat ccgtttaata actactatga
     4441 aaaattagcg aatattaaca tcccaattgc gaatgctggt gatggtagtg gacaacatcc
     4501 aacacaaagt ttacttgatt taatgacgat atatgaagaa tatggatatt ttgaaggctt
     4561 gaatgtattg atttgtggag acattaaaaa ttcacgtgtc gcacgtagta attaccatag
     4621 tttaaaagca ttaggtgcaa acgtaatgtt taatagccca aatgcttgga ttgatgattc
     4681 tctagaagca ccttatgtaa atatagatga tgttatagaa acagtggata tagttatgtt
     4741 attaagaatt caacatgaaa gacatgggct tgcagaagaa actagatttg cagcagatga
     4801 ttatcatcaa aagcatggct taaatgaagt gcgctataac aaattacaag aacatgctat
     4861 tgttatgcat ccggcacctg tgaatagagg agtagaaata caaagcgatt tagtagaagc
     4921 ttcaaaatca agaattttta agcaaatgga aaatggcgtt tacttaagaa tggcagtcat
     4981 tgatgaatta ttaaaatagg taaggggacg aaaatgatga aattaattaa aaacggtaaa
     5041 gtattacaaa atggcgaatt acaacaagca gatattttaa ttgatggtaa ggtaattaaa
     5101 caaattgcac ctgcaattga accaagcaat ggtgttgaca tcatagatgc gaaaggtcac
     5161 tttgtgtcac ctggatttgt cgatgttcat gttcatttac gcgaacctgg tggtgaatat
     5221 aaagagacaa ttgaaactgg tactaaagct gctgctagag gcggatttac aactgtatgt
     5281 ccaatgccta acacaagacc ggtaccagat tctgtagaac attttgaagc tttacaaaaa
     5341 ttaatcgatg acaatgctca agtacgtgta ttaccttatg cttcaattac aacacgtcaa
     5401 ttaggtaaag aattggttga tttcccagca ctagtaaaag aaggtgcctt tgcgtttaca
     5461 gatgacggtg taggagtaca aactgcaagc atgatgtatg aaggcatgat tgaagctgca
     5521 aaagtaaaca aagccatcgt agcacactgt gaagataatt cattaatcta tggtggtgca
     5581 atgcatgaag ggaaacgcag taaagagtta ggtataccag gtattccaaa catttgtgaa
     5641 tctgttcaaa tcgcaagaga tgtactatta gctgaagcag caggttgtca ttatcatgta
     5701 tgtcatgttt ctactaaaga aagtgttaga gtcattcgtg acgctaaacg cgcaggtatt
     5761 catgttacag ctgaagttac accacaccat ttattattaa cagaagatga tattcctggt
     5821 aacaatgcca tttataaaat gaatccacca ttgagaagta ctgaagatag agaggctttg
     5881 ttagaagggt tactagacgg tacaattgac tgtatcgcaa cagaccatgc accacatgca
     5941 cgtgatgaaa aagcgcaacc aatggaaaaa gcaccattcg gaatagttgg tagtgaaaca
     6001 gcattcccat tattatatac gcattttgta aaaaatggtg attggacatt acaacaatta
     6061 gtagattact taacaattaa accatgtgag acatttaatt tagaatacgg cacattaaaa
     6121 gaaaatggtt atgcagattt aacaatcatt gatttagata gtgaacaaga aattaaagga
     6181 gaagatttct tatcaaaagc agataataca ccatttatcg gctataaagt ttatggaaat
     6241 ccgatcttaa caatggttga aggcgaagtt aaatttgagg gggataaata atatgcaaag
     6301 caaacgttat ctagtgttag aagacggttc tttttacgaa ggctaccgtt taggatctga
     6361 taacttaact gtaggagaaa ttgtatttaa tacagcgatg acaggttatc aagaaactat
     6421 ttctgatcca tcatatacag gtcagatcat tacttttacg tatccattaa tcggtaatta
     6481 tggtatcaat agagacgatt ttgaatcatt agtacctaca ttaaacggta ttgtagtgaa
     6541 agaagcgagt gcgcatccaa gtaattttag acagcaaaag acacttcatg acgttttaga
     6601 attgcatcaa attccaggga ttgcaggtgt tgatacaaga agtattacgc gtaaaattcg
     6661 acaacacggt gtgttaaaag ctggttttac tgatcgaaaa gaagatattg atcaacttgt
     6721 caaacattta caacaagtag aattacctaa aaacgaggta gaaatcgttt cgactaaaac
     6781 accgtatgtt tcgacaggta aggatctaag tgtcgtactt gtagactttg gtaagaagca
     6841 aaatattgtt cgagaattaa acgtcagagg ttgtaacgtc acagttgtac catatacaac
     6901 tactgccgaa gaaattttag caatggctcc agatggcgtt atgctatcaa acggaccagg
     6961 taatcctgaa gttgtagaat gtgcgattcc aatgattcaa ggaattttag ggaaaattcc
     7021 gttctttggt atctgtctag gacatcaact ttttgcatta tctcaaggag caagctcatt
     7081 taaaatgaag tttggtcatc gtggtgcgaa ccatccagtt aaaaatttag agactggaaa
     7141 agttgatatt acgagtcaaa accatggata tgcaatagat atagattcgt taaaaagtac
     7201 tgatttagaa gttactcatc ttgcattaaa tgatggtact gtagaaggtt taaaacataa
     7261 aacattacca gcattttctg ttcaatacca tcctgaagca aatccaggac cgtcagattc
     7321 aaactatcta tttgatgatt ttgtagcaat gatgactaat tttaaggaaa aggagcgtca
     7381 tatcaatgcc taaacgtaat gatatcaaaa caattttagt aatagggtct gggccaatta
     7441 tcataggtca agcagctgaa tttgattatg ctggaacaca agcatgtcta gctttaaaag
     7501 aagagggata ccgagttatt cttgtaaatt caaatccagc gacaatcatg actgataagg
     7561 aaattgcgga taaagtatat atcgaaccgt taactcatga ttttatagcg cgaattatac
     7621 gtaaagagca acctgacgct ttacttccaa ctttaggtgg tcaaacaggt ttaaacatgg
     7681 cgattcaact acacgaaagt ggtgtgcttc aagataataa cgtccaatta ttaggaactg
     7741 agctaacatc aattcaacaa gcagaagacc gtgaaatgtt tagaacatta atgaatgatt
     7801 taaacattcc tgtaccagag agtgacattg taaatacagt agagcaagcc tttaaattca
     7861 aagagcaagt gggatacccg ctaattgtta gaccggcatt tacgatgggt ggtaccggag
     7921 gcggtatttg tcataatgat gaagaattac atgaaatcgt ctcaaatggt cttcattata
     7981 gtccagcaac gcaatgttta ttagaaaaat ctatcgcagg ttttaaagaa atcgaatacg
     8041 aagtaatgcg tgataaaaac gataatgcca tcgttgtatg taacatggaa aatattgatc
     8101 cagttggtat tcatacaggc gattcaattg ttgtggctcc tagccaaaca ttatcagatg
     8161 ttgagtatca aatgttacgt gatgtttcat taaaagttat tcgagcttta ggtatcgaag
     8221 gtggttgtaa tgttcaatta gcattagatc cccattcatt cgattattat attatagaag
     8281 taaatccacg tgtatcacgt tcatcagcgt tagcttcaaa agcaacagga tatcctattg
     8341 caaaattagc tgctaaaatc gcggttggtc taacattaga tgaaatgtta aatccaatta
     8401 caggaacatc ttatgcagcg tttgaaccaa ctttagacta tgtgatttca aaaataccaa
     8461 gatttccttt tgataaattt gaaaaaggag aacgagagct tggcacacaa atgaaagcaa
     8521 caggtgaagt tatggccatt ggtcgaactt acgaagaatc attgttaaaa gcaattcgat
     8581 cacttgagta tggtgtgcat cacttaggat taccaaatgg tgaaagcttc gatcttgatt
     8641 atattaaaga acgtatttca caccaagatg atgaacgatt atttttcatc ggcgaagcaa
     8701 ttagaagagg cacaacatta gaagaaattc ataatatgac tcagattgat tacttcttct
     8761 tacacaagtt ccaaaacatt attgatattg agcatcaatt aaaagaacat caaggtgatt
     8821 tagaatatct taaatatgca aaagattatg gatttagtga taaaacaata gcgcatcgct
     8881 ttaatatgac agaagaagaa gtatatcaat tgcgtatgga aaatgatatt aaacctgttt
     8941 acaagatggt tgatacttgc gcagctgaat ttgaatcttc aacaccatat tattatggta
     9001 catacgaaac tgaaaatgaa tccatagtta ctgacaaaga aaaaatctta gtattaggct
     9061 ctggaccaat tcgaatcggc caaggtgtag aatttgacta tgcgacagtt cacgccgttt
     9121 gggcaattca aaaagcaggg tacgaagcga taattgtgaa taacaatcca gaaacagttt
     9181 caacagactt ctcaatttct gacaaattat actttgaacc tttaactgaa gaagatgtga
     9241 tgaatatcat taatttagaa aaacctaaag gtgtcgttgt acaatttgga ggacaaacag
     9301 cgattaattt agcagacaaa ttggctaaac atggtgttaa aatacttggt acttcactag
     9361 aaaatctaaa tcgtgctgaa gatagaaaag aatttgaagc actattaaga aaaattaacg
     9421 tgccacagcc acaagggaaa acagctacat cacctgagga agcattagcg aatgctgcag
     9481 aaatcggata tccggttgta gtaagacctt cttatgtatt aggtggtcgc gcaatggaaa
     9541 ttgtagacaa tgacaaagag ttagaaaact atatgaccca ggctgtaaaa gcgagtccgg
     9601 aacatccggt actagtcgat agatatttaa ctggtaaaga aattgaagtt gatgcgattt
     9661 gtgatggaga aacggtcatt attccaggaa tcatggaaca tattgaacgt gctggtgtgc
     9721 atagtggtga ctcaatcgct gtatatccgc cacaaacttt gacagaagac gagttagcaa
     9781 cacttgagga ctatactata aaattagcta aaggtttaaa catcattggc ttaatcaaca
     9841 ttcaattcgt tatagctcac gatggtgtgt atgttttaga agtaaatcca cgttctagta
     9901 gaacggtacc attcttaagt aaaattactg atattccaat ggcacaatta gctatgcgag
     9961 caatcattgg ggaaaaacta acagatatgg ggtatcaaga aggggttcaa ccatatgctg
    10021 agggtgtatt tgtgaaagca ccggtattta gttttaataa attgaaaaat gttgatatta
    10081 ctttaggacc tgaaatgaag tctacaggtg aagtaatggg gaaagatact acattagaaa
    10141 aggcgttatt caaagggtta acaggtagtg gtgttgaagt taaagatcac ggtacagtat
    10201 taatgaccgt cagtgacaaa gataaagagg aagttgttaa attggcacaa cgcttaaatg
    10261 aagttggcta taaaatttta gcaacgtctg gaacagctaa taaattagct gagtatgaca
    10321 tacctgcaga agtagtaggc aaaattggtg gcgaaaacga tttattaaca cgtattcaaa
    10381 atggtgatgt tcaaatcgtt ataaatacaa tgactaaagg taaagaagta gaaagagatg
    10441 gcttccaaat tagacgtact acagttgaaa atggtattcc atgtttgaca tctttagata
    10501 cagctaatgc cttaacgaat gtaattgaaa gtatgacatt tacaatgcgt caaatgtaaa
    10561 tcaatcaaac tgtatcggtg gggctgtaat taaccattta cttaaagaag tttatattac
    10621 agcctcatta ttttaatgaa ttgcttaacg taaagggaga catatatgat gaaagattta
    10681 ccaattattg cattagattt tgaatcaaaa gaaaaagtaa atcaattttt agatttattt
    10741 gatgaatcat tattcgtaaa agtaggtatg gaactttttt atcaagaagg ccctcaatta
    10801 attaatgaga taaaagaaag aggccatgat gtatttttag atttaaagct gcatgatatt
    10861 cctaatacag ttggtaaggc gatggaaaga ctagctaaat tgaatgttga tctggtaaat
    10921 gttcatgctg ctggtggtgt aaaaatgatg tctgaggcca ttaaaggatt aagaaaacat
    10981 aatcaacata caaaaattat tgcagtaaca cagcttacgt caacaacaga agatatgtta
    11041 cgacacgaac aaaatataca aacatcgatt gaagaggccg ttttaaatta tgccaagtta
    11101 gcaaatgcag ctggtttaga tggcgttgtt tgttcacctc ttgaaagtcg tatgttgact
    11161 gaaaagttag gtacatcatt tttaaaagta acaccaggta ttagacctaa aggtgcatct
    11221 caagatgacc aacaccgtat tacgacaccg gaagaagcaa gacagcttgg ttcgacgcat
    11281 attgtagtcg gtagaccgat tacacaaagt gacaatccag tcgaaagtta tcataaaatt
    11341 aaagaaagtt ggttagtata atggctaaag aaattgcaaa atcattatta gatattgaag
    11401 ctgtaacatt atcaccaaat gatttatata catggagttc aggtattaaa tcaccgattt
    11461 actgtgataa ccgtgttacg ttaggttatc ctttagttcg aggcgcaatc cgcgatggtt
    11521 taattaactt aattaaagaa cactttcctg aagtagaagt tatttctggt actgcaacag
    11581 ctggtattcc acatgcagct tttattgctg aaaaattaaa attaccaatg aattatgttc
    11641 gttcatcaaa taagagtcat ggtaagcaaa atcaaattga aggtgctaaa agtgaaggta
    11701 aaaaagtagt tgtgatagaa gatttaattt cgacaggggg atcttcagtc acagcagttg
    11761 aagccttaaa acaagcaggt gcagaagtat taggtgttgt agctatcttt acttatggtt
    11821 tgaaaaaagc agatgataca tttagcaata ttcaactacc tttttacact ttaagtgatt
    11881 acaatgaatt aattgaagta gctgaaaatg aaggtaaaat ttctagtgaa gatatccaaa
    11941 cattagttga atggagagac aacttagcat aatatagaca ctagaaggag gaattcaaca
    12001 tatgaatgac aaaacatcta atgatttata tgggaagata aaacattgta acgaatttat
    12061 caatcattct aatgattcca atctatctag tagtcacgat gtcgacgaaa gttcaacgaa
    12121 gcaaaaacat ataaaaaata aaacaactat agatcataat gatgatttat ttaaacatgt
    12181 aaaggatata ttacgtaaac aaggacaaat ttaaaaacaa aaaagctata gtgtttaata
    12241 tcatgggctg cttaaccgca tccgaatatt acaaacacta tagcttttat tatataaaaa
    12301 atttattagc ggataattaa ctttattaaa atccaaccaa ttaagaatac gagtaatagg
    12361 actaatactg gaatcacata atgtaacata acgtccctcc tttaacttaa ttttaattgt
    12421 aatcaaattt gacaataagt caaaacatta atacctatga taagtatcat ttattaacat
    12481 atgtatcata tttttaatct tgcgtaattt ttatcgttaa ctatgtgatt taatcaacag
    12541 gccaataaga catttgtcat tcgtgcatat aacggtacga cgcgcttttg tcattttaat
    12601 tatgttaaga taatagtagt tatagaagtt caatctatag gaggcatagc atggatattc
    12661 caaaaatcac gacattttta atgtttaata accaagctga agaagctgtt aaactataca
    12721 caagcttatt tgaagatagt gagattataa caatggctaa gtatggtgaa aatggacctg
    12781 gtgatcctgg gactgtacaa cactcaatat ttacattaaa tggacaagta ttcatggcaa
    12841 ttgatgctaa tagtggcaca gaattaccaa tgaatccagc gatttcatta tttgttacag
    12901 taaaagatac tattgaaatg gaacgactat ttaatggatt aaaagatgaa ggtgccattt
    12961 taatgccaaa aacgaatatg ccaccataca gagagtttgc ttgggttcaa gataagtttg
    13021 gagtaagttt tcaattagca ttacctgagt aaaaggattc gcacagcgtt gaatgataaa
    13081 gaaacacttt ttcttatgca tgcgttttac ctatgtattt ctattttaag tacacattag
    13141 cattttagtt tcactcattt ttaaaatcac aaagataatt tgtaacttaa atgatgatac
    13201 gttatttaaa aacacgatac ttcgtttcaa tgaacgcatt aaataataaa taaacacctc
    13261 gcaaaagaaa gtagtccttt cttatggtga ggtgtttatt tgtcgacaac ttgattatga
    13321 ttgtttcatt ttttgaataa gttcataatc aggtgtagca tacaaagttt tttgattgtc
    13381 atatgttaca aacccaggct ttgcacctga tggtttgtgc acatttttaa ttaacgtgta
    13441 atcaacaggt atttgtccag aattaccagc ttttgaaaaa tatcctgcta acatagccgc
    13501 ttccttgatt gtcgtatcac ttggtgcgtc attaaatata acgacatgtg aaccaggaat
    13561 atcttttgtg tgtaaccatg tgtgagtttt tttagctttt ttatttgtta aataatcatt
    13621 ttgcttgtta ttcttgccaa catatatatc gtcgccatca gttgatacat aatgttgtaa
    13681 ttgaatctgc gctttctttt tcttagtttg atttttacgc tgtttcataa agccttgttc
    13741 tgctaattca tctctaattt catcaatgtc atggacagaa atatgatgta attgttgttc
    13801 gattgttgaa aaataatcta tattgtcttt cgtcaattga atttgatgtt gtaattcacg
    13861 ttctctcgtt ttcatacgat tatattgttt ataataatat tgagcatttg ctgatgggga
    13921 ttttgtagga tttaaaggaa tgacaacttc ttcattcgta taataattca acgccgtcac
    13981 ttctttatca ccttgcttaa ttcgatatat attagcagtg atcaattcac catataactg
    14041 ttcagtatct ttatttttag actgctcata ttcttcaatc aacttcgcta atttattttg
    14101 atatttgtgc aactgctgtt gaacaaatcg aactaaatca ttcgcacgtt gtttaacgcg
    14161 ttcacgttcg ccacgcgcat cataaaaacg atcaagtaaa tcatttaatg aatcgtacgt
    14221 aactgtatca tcattaaatt gatttaactt tataaaatag aaatcctctt tacctgtttc
    14281 atgattttta tgaaaaatag gagtaggtgg taacttggtt tctgccataa cttcgtcgaa
    14341 tgcttctggt aatgttgatg aagtcataaa ttgacgacga ttaacgattt cattcgtaat
    14401 taaagggcta aatccttcaa actgattcaa taattgttta gcaatattac ctgcgttaaa
    14461 atcgatatat ttcaacacct ctgcacctgt aatatcatac ggattcattt tgtgctgagt
    14521 aggtggtgct tcataattaa atcctggcat tactgtacga tagtgattcg tatttggtgt
    14581 taagtgttta aatccttcaa ttattttgcg attttcatct actaaaatta agttactatg
    14641 tttacccata atctctagga taacagtgcg gtaaatagta tcgccaattt catctttact
    14701 ctttatatcg atttcaatgc gacgatcatt accaatttgc ttaatcgatt cgataatacc
    14761 accttctaag tgttttctaa aaacacgcgc aaacatgggt ggattaaatg gattatcata
    14821 ttttttagta gtcaattgta atcttgaaaa gtttggatgg attgacaata acaattgatg
    14881 gttttgtcta ttttgacgta caaccattag tatcgtgtca ttatcaggtt gattgatttt
    14941 gtgaacgcgt cctgttgtta aaaattgtag agactcaacc attttctttg taaataagcc
    15001 atcataagcc ataatttatc ttccaccttc ttcatatcat tcgtttcatt ttaacatgat
    15061 tgtaactttt cggtatagtt attgaaccat acattatgcc acacatttta cattatctaa
    15121 aagtttgcaa ttcaatttat ttaattaaaa ttaatcgtgc aatataacga tattgttaga
    15181 cttaatagaa attatggcat gcaatttcaa atatgctata caatataaag aacaatgtga
    15241 tatcatattt aaataataga agattagctt agagaggtcg taaggcatgg ataatgaaaa
    15301 aggattgtta atcgttttat caggaccatc tggagtaggt aaaggtactg ttagaaaacg
    15361 aatatttgaa gatccaagta catcatataa gtattctatt tcaatgacaa cacgtcaaat
    15421 gcgtgaaggt gaagttgatg gcgtagatta cttttttaaa actagggatg cgtttgaagc
    15481 tttaatcaaa gatgaccaat ttatagaata tgctgaatat gtaggcaact attatggtac
    15541 accagttcaa tatgttaaag atacaatgga cgaaggtcat gatgtatttt tagaaattga
    15601 agtagaaggt gcaaagcaag ttagaaagaa atttccagat gcattattta ttttcttagc
    15661 acctccaagt ttagatcact tgagagagcg attagtaggt agaggaacag aatccgatga
    15721 gaaaatacaa agtcgtatta acgaagcacg taaagaagtc gaaatgatga atttatacga
    15781 ttacgttgta gttaatgatg aagtagaact tgcgaagaat agaattcaat gtattgtaga
    15841 agctgagcac ttaaaaagag agcgcgtaga agctaagtat agaaaaatga ttttggaggc
    15901 taaaaaataa tgttaaatcc accattaaac caattaacgt cacaaattaa atcaaagtat
    15961 ttaattgcaa caactgcagc gaaaagagcg cgtgaaattg atgaacaacc tgaaactgaa
    16021 ttattaagtg aatatcattc atttaaacca gttggtagag cgttagaaga aattgctgac
    16081 ggtaaaattc gccctgttat ttcaagcgat tattatggta aagaatagtt tcgaccatta
    16141 aaaatatgtg aagctagacg tacatcaaat acgtcaaggc atatccaaat caaacgcacc
    16201 tatgtaggtg tgttttttta gtttatccaa tgattgatgt tataataata ctaaatttgt
    16261 atctataaaa aagtaatgag catttgtgcg catatgatga tgtaaagcgt aaatgtcata
    16321 gtgatattta agggagatta aatatgaaga aaatattatt agccgttaca ggtggcattg
    16381 cggcatataa agcaattgat ttgacaagta agttaacaca atctgggtat gaagttcgcg
    16441 ttatgttaac gaatcacgca caaaaatttg tgacaccatt agcatttcaa gcaataagtc
    16501 gaaatgctgt ttatacagat acttttatag aagaaaatcc ttcagaaata cagcatattg
    16561 cattaggtga ttgggcagat gcaatcattg ttgcacctgc aacggcaaat acaattgcaa
    16621 aattgagtgt aggtattgct gatgatttgg tgacatcaac gttgctagca acagagacac
    16681 cgaaatttat tgcgcctgct atgaatgtgc atatgtatga aaataaacgt acgcagcaaa
    16741 atattaatat tttaaaagaa gatgggtatc attttatcga accaggaagc ggatttctag
    16801 catgtggtta tgttgctaaa ggacgtatgg aagaaccgct tcaaatcgtt tctgttattg
    16861 atgctcattt tcaaaatagt aatcgtttag ctaatagttc atttcaagat aaacgcgcat
    16921 tggttacagc aggaccaact attgaagtta tcgatccagt cagatttgta tccaatcgtt
    16981 cttctggaaa aatgggctat gcaatagctg aagcattgcg aaatcgagga gctatcgtga
    17041 cgttagttgc tggtcctaca acactagagg atccaaaaga tattgaagtt attcatgttc
    17101 aaagtgctga agaaatgttt gaacaagtga caagccgatt tgacgaacaa gatattgttg
    17161 taaaagcagc agccgtatct gactatacac cagttgatgt attagaacat aagatgaaaa
    17221 agcaggatgg tgatttatca gtatctttta aacgtactaa agacattctt aaatatttag
    17281 gtgaacataa aacatcacag tatttaatag gctttgcagc agagactgaa gatattgaaa
    17341 attatgcaca acaaaaatta cgcaagaaaa atgcagatgt gattatttca aataatgttg
    17401 gggatatgtc tatcggattt agttctgatg ataatgaatt gacaatgcat tttaaaaata
    17461 atgaaaaggt aaatatcaag aaaggaaaaa aagtagtatt agctgcacaa attttagatg
    17521 aactagaaac taggtggcaa taatgatagc gaaagtcata gtcgatgtcg cgtcgaagag
    17581 cgttgacttt aaatttgatt atataattcc tgaacaactc gaatctgtca tccaacctgg
    17641 tgtgcgtgtg attgtacctt ttggaccaag aacgattcaa ggttatgtaa tggaagtaac
    17701 agcagaacct gatgcacaac ttgacgtttc gaagttaaaa aaaatcatag aagtgaaaga
    17761 tatacaacct gaattaacat cagaattaat agctttaagt gagtggatgg gttcaactca
    17821 tgtcattaaa cgtatttcta tgctagaagt gatgcttccg agtgctatta aagcgaagta
    17881 taaaaaagca tttaagatga aagatgacat agagctacct tcagctttat tacaaaaatt
    17941 tgataagcat ggttactatt attataaaga tgcgcaaaaa aataatgata ttcaattgct
    18001 tatgaagttg ttaaaagatg atatcgttga agaaaaaacg attctcacac aaaatataac
    18061 taaaaaaacc aagcgtgctg ttcgtgtcat tgaagggtat catcctgatg aagtattagc
    18121 taagttggag aaagttatta aacaatacga tttgtatgct tacttgtctg aagaacaaca
    18181 taaaacaata tttttaactg atattgagga tatgggcttt tcaaaatcca gtttagatgg
    18241 acttatcaaa aaaggttatg ttgaaaaata tgacgcggtt gttgatagag acccatttaa
    18301 agatcgtgtt ttcgaacaag aatcaaaaca gcaattaaca gaagatcaat ataaagcata
    18361 tgaagcgatt aaagctaaaa ttgtaagcca agagcaagaa acatttttac ttcatggtgt
    18421 gacgggatca ggtaaaacag aagtatattt acaaacgata gaagatgttt taagccaagg
    18481 aaaacaggcg atgatgttag ttcctgaaat cgctctaaca ccgcaaatgg ttttacgctt
    18541 caaacgtcga tttggtgatg acgttgctgt attacattct ggcttatcta atggggaacg
    18601 ttatgatgag tggcaaaaaa ttagggatgg tcgtgcgaga gtaagtgttg gtgcaaggtc
    18661 aagtgtgttc gcacctttca aaaatttagg gttaatcatc attgatgaag aacatgaatc
    18721 tacatataaa caagaagatt atccgagata tcacgctaga gaaattgccc aatggcgaag
    18781 tgaatatcat cactgtccag tcattttagg aagtgcaaca ccatgtcttg aaagttatgc
    18841 acgagctgaa aaaggcgttt atcatttgct atcattacca aacagagtga accaacaagc
    18901 tttacctgaa attgatatag tagacatgcg tgaagaattg agtgaaggta atcggtcaat
    18961 gttttcaaaa gatttacgtg aagccataca attaagatta gatcgacagg aacaagttgt
    19021 tttattttta aatcgacgtg gttatgcatc gtttatgtta tgtcgggatt gtggatatgt
    19081 accgcaatgt ccaaactgtg atatttcatt aacgtatcat aaaacgacag acttattaaa
    19141 atgtcactat tgtggttacc aagagacgcc accgaatcaa tgtccaaatt gtgagagtga
    19201 acacattcga caagtaggta ctggtactca gaaagttgaa gaactattgc aacaagaatt
    19261 tgaagatgcg cgcataatta ggatggatgt agatacaacc tcaaagaaag gtgcacatga
    19321 aaagttattg actgaattcg aaaaaggtaa cggtgacatt ttactaggta ctcagatgat
    19381 tgcgaaagga ttagattatc caaatattac tttagttggt gtgctgaatg cagatacaat
    19441 gttaaattta cctgattttc gggcgagcga acgtacttat caactattaa cgcaagtggc
    19501 tggtagagct ggtcgtcatg aaaaggcagg tcaagtcatc attcaaacgt ataatccaga
    19561 tcattattca atattggatg ttcaaaaaaa tgattattta acattttatc gtcaggaaat
    19621 ggaatatcgt aaattaggaa agtatccacc gtattattat ttgattaatt tcacaatctc
    19681 acataaagaa atgaagaagg ttatggaagc atcgcagcat gttcataaaa ttttattaca
    19741 gcatttaaca gaaaaagcgc ttgtgctagg tccatctccg gcagcacttg cgagaatcaa
    19801 caatgaattt agattccaaa ttttagtgaa atataaaagt gaacctggat tattacaagc
    19861 cattcagttt ttagatgact attaccatga aaaatttata aaagaaaaat tagcattgaa
    19921 gattgatatt gatccgcaga tgatgatgta acattactaa ttattagaaa caagttcagt
    19981 atgtacgagt atttgaacca gtgtttgaat atttacttta agtacagaat aagggcagaa
    20041 tatacaactg ttaactattt aaattagcag tttatattct gtctttttat atggctttat
    20101 aacttacgtg attttggttt gataaggaat ttattagtat tttcatttac gaattctgat
    20161 tttaatgcaa gcgccatact tactgatgaa taaaagtact aatgattaag
//
LOCUS       DAHHOJ010000044        20025 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.044, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000044 DAHHOJ010000000
VERSION     DAHHOJ010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 20025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20025
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.044"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            143..715
                     /locus_tag="NBH32_001350"
     CDS             143..715
                     /locus_tag="NBH32_001350"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCD1514964.1"
                     /translation="MKCIVGLGNIGKCFELTRHNIGFEVVDYILEKNNFSLDKQKFKG
                     AYTIERMNGDKVLFIEPMTMMNLSGEAVAPIMDYYNVNPEDLIVLYDDLDLEQGQVRL
                     RQKGSAGGHNGMKSIIKMLGTDQFKRIRIGVGRPTNGMTVPDYVLQRFSNDEMVTMEK
                     VIEHAARAIEKFVETSRFDHVMNEFNGEVK"
     gene            715..4221
                     /gene="mfd"
                     /locus_tag="NBH32_001351"
     CDS             715..4221
                     /gene="mfd"
                     /locus_tag="NBH32_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCD1514965.1"
                     /translation="MTILTTLIKEDNHFQDLNQVFGQANTLVTGLSPSAKVTMIAEKY
                     AQSNQQLLLITNNLYQADKLETDLLQFIDAEELYKYPVQDIMTEEFSTQSPQLMSERI
                     RTLTALAQGKKGLFIVPLNGLKKWLTPVEMWQNHQMTLRVGEDIDVDQFLNKLVNMGY
                     KRESVVSHIGEFSLRGGIIDIFPLIGEPIRIELFDTEIDSIRDFDVETQRSKDNVEEV
                     DITTASDYIITEEVISHLKEELKTAYENTRPKIDKSVRNDLKETYESFKLFESTYFDH
                     QILRRLVAFMYETPSTIIEYFQKDAIIAVDEFNRIKETEESLTVESDSFISNIIESGN
                     GFIGQSFIKYDDFETLIEGYPVTYFSLFATTMPIKLNHIIKFSCKPVQQFYGQYDIMR
                     SEFQRYVNQNYHIVVLVETETKVERMQAMLSEMHIPSITKLHRSMSSGQAVIIEGSLS
                     EGFELPDMGLVVITERELFKSKQKKQRKRTKAISNAEKIKSYQDLNVGDYIVHVHHGV
                     GRYLGVETLEVGQTHRDYIKLQYKGTDQLFVPVDQMDQVQKYVASEDKTPKLNKLGGS
                     EWKKTKAKVQQSVEDIAEELIDLYKEREMAEGYQYGEDTAEQTTFELDFPYELTPDQA
                     KSIDEIKDDMQKSRPMDRLLCGDVGYGKTEVAVRAAFKAVMEGKQVAFLVPTTILAQQ
                     HYETLIERMQDFPVEIQLMSRFRTPKEIKQTKEGLKTGFVDIVVGTHKLLSKDIQYKD
                     LGLLIVDEEQRFGVRHKERIKTLKHNVDVLTLTATPIPRTLHMSMLGVRDLSVIETPP
                     ENRFPVQTYVLEQNMSFIKEALERELSRDGQVFYLYNKVQSIYEKREQLQMLMPDANI
                     AVAHGQMTERDLEETMLSFINNEYDILVTTTIIETGVDVPNANTLIIEDADRFGLSQL
                     YQLRGRVGRSSRIGYAYFLHPANKVLTETAEDRLQAIKEFTELGSGFKIAMRDLNIRG
                     AGNLLGKQQHGFIDTVGFDLYSQMLEEAVNEKRGIKEPESEVPEVEVDLNLDAYLPTE
                     YIANEQAKIEIYKKLRKTETFDQIIDIKDELIDRFNDYPVEVARLLDIVEIKVHALHS
                     GITLIKDKGKIIDIHLSVKATENIDGEVLFKATQPLGRTMKVGVQNNAMTITLTKQNQ
                     WLDSLNFLVKCIEESMRISDEA"
     gene            4211..5737
                     /locus_tag="NBH32_001352"
     CDS             4211..5737
                     /locus_tag="NBH32_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1514966.1"
                     /translation="MKHKEAFNGVVVLTAALIVIKILSAVYRIPYQNILGDTGLYAYQ
                     QVYPIVALGMILSMNAIPSAITQNIGKYHSDEAYAKAVAYIQLVGILIFIAIFVFANN
                     IAHMMGDGHLTPMIQAASLSFIFIGMLGVLRGYYQSANNMTVPAISQVIEQVIRVGII
                     IVTIVIFVDRGWTIYEAGTIAILASTIGFLGSSIYLVAHRPFKFKMVNNTAKIVWKQF
                     ALSVLIFAISQLIVILWQVIDSVTIIKSLQAIRVPFDVAITEKGVYDRGASFIQMGLI
                     VTTTFSFALIPLLSDAIKMNNQVLMNRYANASLKITILISTAAGIGLINLLPLMNGVF
                     FKTNDLTLTLSVYMITVICVSLIMMDMALLQAQHAVRPIFVGMTAGLVIKFILNIILI
                     RLSGIIGASISTVVSLIIFGTIIHIAVTRKYHLHAMRRFFINVVLGMVFMSIVVQCVL
                     NIVTTHGRITGLIELLCAAVLGIIALFFYIFRFNVLTYKELTYLPFGSKLYQIKKGRR
                     "
     gene            5737..6930
                     /locus_tag="NBH32_001353"
     CDS             5737..6930
                     /locus_tag="NBH32_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1514967.1"
                     /translation="MAHTITIVGLGNYGIDDLPLGIYKFLKTQDKVYARTLDHPVIES
                     LQDELTFQSFDHVYEAHDQFEDVYNDIVAQLVEAANEKDIVYAVPGHPRVAETTTVKL
                     LALAKDNTDIDVKVLGGKSFIDDVFEAVNVDPNDGFTLLDATSLQEVTLNVRTHTLIT
                     QVYSAMVAANLKITLMERYPDDYPVQIVTGARSDGADNVVTCPLYELDHDENAFNNLT
                     SVFVPKIITSTYLYHDFDFATEVIDTLVDEDKGCPWDKVQTHETLKRYLLEETFELFE
                     AIDNEDDWHMIEELGDILLQVLLHTSIGKKEGYIDIKEVITSLNAKMIRRHPHIFGDA
                     NAETIDDLKEIWSKAKDAEGKQPRVKFEKVFAEHFLNLYEKTKDKSFDEAALKQWLEK
                     GESNT"
     gene            6927..7190
                     /locus_tag="NBH32_001354"
     CDS             6927..7190
                     /locus_tag="NBH32_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HCD1514968.1"
                     /translation="MRLDKYLKVSRLIKRRTLAKEVSDQGRITINGNVAKAGSDVKVE
                     DVLTIRFGQKLVTVKVTALNEHASKDNAKGMYEIVEERRLEEA"
     gene            7208..7600
                     /locus_tag="NBH32_001355"
     CDS             7208..7600
                     /locus_tag="NBH32_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HCD1514969.1"
                     /translation="MKNKVEHIENQYTSQENKKKQRQKMKMRVVRRRITVFAGVLLAI
                     IVVLSILLVVQKHRNDIDAQERKAKEAQFQKQQNEEIALKEKLNNLNDKDYIEKIARD
                     DYYLSNKGEVIFRLPEDKDSSSSKSSKK"
     gene            7705..8106
                     /locus_tag="NBH32_001356"
     CDS             7705..8106
                     /locus_tag="NBH32_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HCD1514970.1"
                     /translation="MSIEVGNKLKGKVTGIKKFGAFVELPEGKSGLVHISEVADNYVE
                     NVEEHLSVGDEVDVKVLSIADDGKISLSIKKAKDRPRRQHTSKPSHQKPVQKAEDFEK
                     KLSNFLKDSEDKLTSIKRQTESRRGGKGSRR"
     gene            8286..9581
                     /gene="tilS"
                     /locus_tag="NBH32_001357"
     CDS             8286..9581
                     /gene="tilS"
                     /locus_tag="NBH32_001357"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCD1514971.1"
                     /translation="MQLNSNGWHVDDHIVVAVSTGIDSMCLLYQLLNDYKDSYRKLTC
                     LHVNHGVRSASIEEARFLEVYCERHHIDLHIKKLDLSHSLDRNNSIQNEARIKRYEWF
                     DEMMNVLEADVLLTAHHLDDQLETIMYRIFNGKSTRNKLGFDELSKRKGYQIYRPLLA
                     VSKKEIKQFQERYHIPYFEDESNKDNKYVRNDIRNRIIPAIDENNQLKVSHLLKLKQW
                     HDEQYDILQYSAKQFIQEFVKFDEQSKYLEVSRQAFNNLPNSLKMVVLDCLLSKYYEL
                     FNISAKTYEEWFKQFSSKKAQFSINLTDKWIIQIAYGKLIIMAKNNGDTYFRVQTIEK
                     PGNYIFNKYRLEIHSNLPKCLFPLTVRTRQSGDTFKLNGRDGYKKVNRLFIDCKVQQW
                     VRDQMPIVLDKQQRIIAVGDLYQQQTIKQWIIISKNGDE"
     gene            9586..10125
                     /gene="hpt"
                     /locus_tag="NBH32_001358"
     CDS             9586..10125
                     /gene="hpt"
                     /locus_tag="NBH32_001358"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCD1514972.1"
                     /translation="MHNDLKEVLLTEEDIQNICKELGAQLTKDYQGKPLVCVGILKGS
                     AMFMSDLIKRIDTHLSIDFMDVSSYHGGTESTGEVQIIKDLGSSIENKDVLIIEDILE
                     TGTTLKSITELLQSRKVNSLEIVTLLDKPNRRKADIEAKYVGKKIPDEFVVGYGLDYR
                     ELYRNLPYIGTLKPEVYSN"
     gene            10382..12475
                     /gene="ftsH"
                     /locus_tag="NBH32_001359"
     CDS             10382..12475
                     /gene="ftsH"
                     /locus_tag="NBH32_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCD1514973.1"
                     /translation="MQKAFRNVLVIVIIGVIIFGLFSYLNGNGNMPKQLTYNQFTEKL
                     EKGDLKTLEIQPQQNVYMVSGKTKNDEDYSSTILYNNEKELQKITDAAKKQNGVKLTI
                     KEEEKQSVFVSILSTLIPVVVIALLFIFFLSQAQGGGSGGRMMNFGKSKAKMYDNNKR
                     RVRFSDVAGADEEKQELIEIVDFLKDNKKFKEMGSRIPKGVLLVGPPGTGKTLLARAV
                     AGEAGAPFFSISGSDFVEMFVGVGASRVRDLFDNAKKNAPCIIFIDEIDAVGRQRGAG
                     VGGGHDEREQTLNQLLVEMDGFGENEGIIMIAATNRPDILDPALLRPGRFDRQIQVGR
                     PDVKGREAILHVHAKNKPLDETVDLKAISQRTPGFSGADLENLLNEASLIAVREGKKK
                     IDMRDIEEATDRVIAGPAKKSRVISKKERNIVAHHEAGHTIIGMVLDEAEVVHKVTIV
                     PRGQAGGYAMMLPKQDRFLMTEQELLDKICGLLGGRVSEDINFNEVSTGASNDFERAT
                     QIARSMVTQYGMSKKLGPLQFGHSNGQVFLGKDMQGEPNYSSQIAYEIDKEVQRIVKE
                     QYERCKQILLEHKEQLILIAETLLTEETLVAEQIQSLFYEGKLPEIDYDAAKVVKDED
                     SEFNDGKFGKSYEEIRKEQLEDGQRDESEDRKEEKDIAEDKKEADKSDEKDEPAHRQA
                     PNIEKPYDPNHPDNK"
     gene            12704..13585
                     /gene="hslO"
                     /locus_tag="NBH32_001360"
     CDS             12704..13585
                     /gene="hslO"
                     /locus_tag="NBH32_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCD1514974.1"
                     /translation="MTHDYIVKALAFDGEIRAYAALTTETVQEAQTRHYTWPTASAAM
                     GRTMTATAMMGAMLKGDQKLTVTVDGQGPIGRIIADANAKGEVRAYVDHPQTHFPLNE
                     QGKLDVRRAVGTNGSIMVVKDVGMKDYFSGASPIVSGELGEDFTYYYATSEQTPSSVG
                     LGVLVNPDNTIKAAGGFIIQVMPGAKDETISKLEKAISEMTPVSKLIEQGLTPEGLLN
                     EILGEDHVQILEKMPVQFECNCSHEKFLNAIKGLGEAEIQNMIKEDHGAEAVCHFCGN
                     KYKYTEEELNVLLESLA"
     gene            13764..14696
                     /gene="cysK"
                     /locus_tag="NBH32_001361"
     CDS             13764..14696
                     /gene="cysK"
                     /locus_tag="NBH32_001361"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCD1514975.1"
                     /translation="MAQKPVDNITQIIGDTPVVKLRNVVDDNAADVYVKLEYQNPGGS
                     VKDRIALAMIEKAEREGKIKPGDTIVEPTSGNTGIGLAFVCAAKGYKAVFTMPETMSQ
                     ERRNLLKAYGAELVLTPGSEAMKGAIKKAKELKEEHGYFEPQQFENPANPEVHELTTG
                     PELLQQFEGKTIDAFLAGVGTGGTLSGVGKVLKKEYPNIEIVAIEPEASPVLSGGEPG
                     PHKLQGLGAGFIPGTLNTEIYDSIIKVGNDTAMEMSRRVAKEEGILAGISSGAAIYAA
                     IQKAKELGKGKTVVTVLPSNGERYLSTPLYSFDD"
     gene            14912..15715
                     /gene="folP"
                     /locus_tag="NBH32_001362"
     CDS             14912..15715
                     /gene="folP"
                     /locus_tag="NBH32_001362"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCD1514976.1"
                     /translation="MTKTKIMGILNVTPDSFSDGGKFNNVESAVTRVKAMMDEGADII
                     DVGGVSTRPGHEMVSLEEELNRVLPVVEAIVGFDVKISVDTFRSEVAEACLKLGVDII
                     NDQWAGLYDHHMFQIVAKYDAEIILMHNGNGNRDEPVVEEMLTSLLAQAHQAKIAGIP
                     SNKIWLDPGIGFAKTRNEEAEVMARLDELVATEYPVLLATSRKRFTKEMMGYDTTPVE
                     RDEVTAATTAYGIMKGVRAVRVHNVELNAKLAKGIDFLKENENARHNLS"
     gene            15693..16058
                     /gene="folB"
                     /locus_tag="NBH32_001363"
     CDS             15693..16058
                     /gene="folB"
                     /locus_tag="NBH32_001363"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HCD1514977.1"
                     /translation="MQDTIFLKGMRFYGYHGALSAENEIGQIFKVDVTLKVDLAEAGR
                     TDNVIDTVHYGEVFEEVKSIMEGKAVNLLEHLAERIANRINSQYNRVMETKVRITKEN
                     PPIPGHYDGVGIEIVRENK"
     gene            16055..16531
                     /gene="folK"
                     /locus_tag="NBH32_001364"
     CDS             16055..16531
                     /gene="folK"
                     /locus_tag="NBH32_001364"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCD1514978.1"
                     /translation="MIQAYLGLGSNIGDRESQLNDAIKILNEYDGINVSNISPIYETA
                     PVGYTEQPNFLNLCVEIQTTLTVLQLLECCLKTEECLHRVRKERWGPRTLDVDILLYG
                     EEMIDLPKLSVPHPRMNERAFVLIPLNDIAANVIEPRSKLKVKDLVFVDDSVKRYK"
     gene            16925..17017
                     /locus_tag="NBH32_001365"
     CDS             16925..17017
                     /locus_tag="NBH32_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514979.1"
                     /translation="MVNDKVLETSKEMYVEQKCLIFYKTLKENV"
     gene            17070..18557
                     /gene="lysS"
                     /locus_tag="NBH32_001366"
     CDS             17070..18557
                     /gene="lysS"
                     /locus_tag="NBH32_001366"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCD1514980.1"
                     /translation="MSEEMNDQMLVRRQKLQELYDLGIDPFGSKFDRSGLSSDLKEEW
                     DQYSKEELVEKEADSHVAIAGRLMTKRGKGKAGFAHVQDLAGQIQIYVRKDQVGDDEF
                     DLWKNADLGDIVGVEGVMFKTNTGELSVKAKKFTLLTKSLRPLPDKFHGLQDIEQRYR
                     QRYLDLITNEDSTRTFINRSKIIQEMRNYLNNKGFLEVETPMMHQIAGGAAARPFVTH
                     HNALDATLYMRIAIELHLKRLIVGGLEKVYEIGRVFRNEGVSTRHNPEFTMIELYEAY
                     ADYHDIMDLTESMVRHITNEVLGSAKVQYNGETIDLESAWTRLHIVDAVKEATGVDFY
                     EVKSDEEAKALAKEHGIEIKDTMKYGHILNEFFEQKVEETLIQPTFIYGHPTEISPLA
                     KKNPEDPRFTDRFELFIVGREHANAFTELNDPIDQKGRFEAQLVEKAQGNDEAHEMDE
                     DYIEALEYGMPPTGGLGIGIDRLVMLLTDSPSIRDVLLFPYMRQK"
     gene            19115..19229
                     /gene="rrf"
                     /locus_tag="NBH32_001367"
     rRNA            19115..19229
                     /gene="rrf"
                     /locus_tag="NBH32_001367"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            19242..19317
                     /locus_tag="NBH32_001368"
     tRNA            19242..19317
                     /locus_tag="NBH32_001368"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19275..19277,aa:Val,seq:tac)
     gene            19334..19409
                     /locus_tag="NBH32_001369"
     tRNA            19334..19409
                     /locus_tag="NBH32_001369"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19367..19369,aa:Thr,seq:tgt)
     gene            19416..19488
                     /locus_tag="NBH32_001370"
     tRNA            19416..19488
                     /locus_tag="NBH32_001370"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19449..19451,aa:Lys,seq:ttt)
     gene            19522..19596
                     /locus_tag="NBH32_001371"
     tRNA            19522..19596
                     /locus_tag="NBH32_001371"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19554..19556,aa:Gly,seq:gcc)
     gene            19604..19692
                     /locus_tag="NBH32_001372"
     tRNA            19604..19692
                     /locus_tag="NBH32_001372"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19638..19640,aa:Leu,seq:taa)
     gene            19698..19774
                     /locus_tag="NBH32_001373"
     tRNA            19698..19774
                     /locus_tag="NBH32_001373"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19732..19734,aa:Arg,seq:acg)
     gene            19795..19868
                     /locus_tag="NBH32_001374"
     tRNA            19795..19868
                     /locus_tag="NBH32_001374"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19829..19831,aa:Pro,seq:tgg)
     gene            19892..19967
                     /locus_tag="NBH32_001375"
     tRNA            19892..19967
                     /locus_tag="NBH32_001375"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19925..19927,aa:Ala,seq:tgc)
BASE COUNT         7091 a         2723 c         4104 g         6107 t
ORIGIN      
        1 tacatcataa aaatttttat gtactcgatt ttaaaataca tacttactaa gctaaagaat
       61 aatgataatt gatggcaatg gcggaaaatg gatgttgtca ttataataat aaatgaaaca
      121 attatgttgg aggtaaacac gcatgaaatg tattgtaggt ctaggtaata taggtaaatg
      181 ttttgaactt acaagacata atatcggctt tgaagtcgtt gattatattt tagagaaaaa
      241 taatttttca ttagataaac aaaagtttaa aggtgcatat acaattgaac gaatgaacgg
      301 cgataaagtg ttatttatcg aaccaatgac aatgatgaat ttgtcaggtg aagcagttgc
      361 accgattatg gattattaca atgttaatcc agaagattta attgtcttat atgatgattt
      421 agatttagaa caaggacaag ttcgcttaag acaaaaagga agtgcgggcg gtcacaatgg
      481 tatgaaatca attattaaaa tgcttggtac agaccaattt aaacgtattc gtattggtgt
      541 gggaagacca acgaatggta tgacggtacc tgattatgtt ttacaacgct tttcaaatga
      601 tgaaatggta acgatggaaa aagttatcga acacgcagca cgcgcaattg aaaagtttgt
      661 tgaaacatca cgatttgacc atgttatgaa tgaatttaat ggtgaagtga aataatgaca
      721 atattgacaa cgcttataaa agaagataat cattttcaag accttaatca ggtatttgga
      781 caagcaaaca cactagtaac tggtctttcc ccgtcagcta aagtgacgat gattgctgaa
      841 aaatatgcac aaagtaatca acagttatta ttaattacca ataatttata ccaagcagat
      901 aaattagaaa cagatttact tcaatttata gatgctgaag aattgtataa gtatcctgtg
      961 caagatatta tgaccgaaga gttttcaaca caaagccctc aactgatgag tgaacgtatt
     1021 agaactttaa ctgcgttagc tcaaggtaag aaagggttat ttatcgttcc tttaaatggt
     1081 ttgaaaaagt ggttaactcc tgttgaaatg tggcaaaatc accaaatgac attgcgtgtt
     1141 ggtgaggata tcgatgtgga ccaatttctt aacaaattag ttaatatggg gtacaaacgg
     1201 gaatccgtgg tatcgcatat tggtgaattc tcattgcgag gaggtattat cgatatcttt
     1261 ccgctaattg gggaaccaat cagaattgag ctatttgata ccgaaattga ttctattcgg
     1321 gattttgatg ttgaaacgca gcgttccaaa gataatgttg aagaagtcga tatcacaact
     1381 gcaagtgatt atatcattac tgaagaagtg atcagccatc ttaaagaaga gttaaaaact
     1441 gcatatgaaa atacaagacc caaaatagat aaatcagtgc gcaatgattt gaaagaaacg
     1501 tatgaaagct ttaaattatt cgaaagtaca tactttgatc atcaaatact acgtcgttta
     1561 gtagcgttta tgtatgaaac accttcgaca attattgagt atttccaaaa agatgcaatc
     1621 attgcagttg atgaatttaa tcgtattaaa gaaactgaag aaagtttaac agtagagtct
     1681 gattcgttta ttagcaatat tattgaaagt ggtaatggat ttataggaca aagttttata
     1741 aaatatgatg attttgaaac attgattgaa ggctatcctg tcacttattt ttcattattc
     1801 gctacaacaa tgccgataaa actaaatcat attattaaat tttcatgtaa acctgtccaa
     1861 caattttatg ggcaatatga cattatgcgt tctgaatttc aacgatatgt caatcaaaat
     1921 tatcatattg tggttttggt cgaaactgaa actaaagttg aacgtatgca agcgatgtta
     1981 agtgaaatgc atattccatc aataacaaaa ttgcatcgct caatgtcatc ggggcaagca
     2041 gtgattattg aaggcagttt atctgaagga tttgaactac ctgatatggg attagttgtc
     2101 attactgagc gtgagctttt taaatcaaaa cagaaaaagc aacgaaaacg tacgaaagct
     2161 atctcaaatg ctgaaaaaat taagtcttac caagatttaa atgtgggaga ttatattgtt
     2221 catgtgcatc atggtgttgg tagatattta ggtgttgaga cgctcgaagt ggggcaaacg
     2281 catcgggatt atattaaatt gcaatataaa ggtacggatc aactatttgt tccagtagat
     2341 caaatggatc aagttcaaaa atatgtagct tcggaagata agacgccaaa attaaataaa
     2401 ctcggtggca gtgaatggaa aaaaacaaaa gctaaagttc aacaaagtgt tgaagatatt
     2461 gctgaagagt tgattgattt atataaagaa agagaaatgg cagaaggtta tcaatatggg
     2521 gaagacacag ctgagcaaac aacatttgaa ttagattttc catatgaact tacgcctgac
     2581 caagctaaat ctatcgatga aattaaagat gacatgcaaa aatcgcgtcc aatggatcgc
     2641 ttgctatgtg gtgatgttgg ttatggtaaa actgaagttg cagtgagagc agcattcaaa
     2701 gctgtaatgg aaggaaagca agttgcattt ttagttccta caactatttt agctcagcaa
     2761 cattatgaga cgttaattga gcgtatgcaa gattttcctg ttgaaattca attaatgagt
     2821 cgttttagaa cgccaaaaga gataaaacaa actaaggaag gacttaaaac tgggtttgtt
     2881 gacatagttg ttggtacaca caaattactt agtaaagata tacagtataa agatttaggg
     2941 ctgttgattg tagatgaaga acaacgattt ggtgtacgcc ataaagagcg tattaaaaca
     3001 ttaaaacata atgtagatgt actaacattg actgcaaccc caatacctag aacattgcat
     3061 atgagtatgc taggtgtgcg cgatttgtca gtgattgaaa cgccgccaga aaatcgtttc
     3121 ccagttcaaa catatgtatt agaacagaac atgagtttta tcaaagaagc tttagaaaga
     3181 gaactatccc gtgatggcca agtgttttat ctttataata aagtgcaatc catttatgaa
     3241 aaacgagaac aactccagat gttaatgcca gatgctaaca ttgcagttgc tcatggacaa
     3301 atgacagagc gcgatttaga agaaacgatg ttaagtttta tcaataatga atatgatatt
     3361 ttagtaacga cgacgattat tgaaacaggt gtcgatgtcc caaatgcaaa tactttgatc
     3421 attgaagatg cagatcgctt tggattgagt cagttgtatc aattaagagg tcgtgttggt
     3481 cgttcaagtc gtattggtta tgcatacttc ctacatccag caaataaggt actaactgag
     3541 actgcagaag atcgattaca agcgattaaa gaatttacgg agttaggctc aggatttaag
     3601 attgcgatgc gtgatttgaa cattcgtggt gctggtaatt tgttaggtaa acaacagcac
     3661 ggctttattg atacagttgg atttgatttg tacagtcaaa tgttagaaga agctgtaaat
     3721 gaaaaacgtg gtattaagga accagaatct gaggtgccag aagtcgaagt tgatttaaac
     3781 ttggatgcat atttgccaac agagtatatt gcaaatgaac aagctaaaat tgaaatttat
     3841 aaaaagctac gaaaaactga aacatttgat caaattatcg acattaaaga tgaattaatt
     3901 gatcgtttta atgattatcc tgttgaagta gcacgtttgc ttgatatagt ggaaataaaa
     3961 gtacacgcat tacattcagg tatcacgttg attaaagata aagggaaaat aattgatatt
     4021 catttatctg taaaagccac tgaaaatatt gatggcgaag tgctgttcaa agcaacacaa
     4081 cctttaggta gaacaatgaa ggttggtgtt caaaataatg caatgacaat tactttaacg
     4141 aaacaaaatc aatggcttga tagtttgaat tttttagtta agtgcattga agaaagtatg
     4201 agaatcagtg atgaagcata aagaagcatt taatggcgtt gtcgtgttaa ctgctgcatt
     4261 aattgtcatt aaaattctga gtgctgtata tcgaattcca tatcaaaata tattaggcga
     4321 tacaggtttg tatgcatatc aacaagtgta tccaattgta gcactaggaa tgatattatc
     4381 gatgaatgcc attcctagtg caattacaca aaatatagga aagtatcata gtgacgaagc
     4441 atatgcaaaa gcagtcgctt atatacaatt agttggtata ttaatattta ttgctatttt
     4501 tgtgtttgcg aacaatattg cacatatgat gggtgatggc catttaacac caatgattca
     4561 agctgcaagt ttaagcttta tatttatagg tatgcttggc gtgttaagag gttattatca
     4621 atctgcaaat aatatgacag ttccggctat ttcccaggtt atagaacaag ttatacgagt
     4681 aggtattatc attgttacta ttgttatttt tgtagacaga ggttggacga tatatgaagc
     4741 gggaacaatt gctattttag catcaacgat aggtttttta ggttcttcaa tttatttagt
     4801 agcgcaccga ccttttaagt ttaaaatggt aaataacact gcaaagattg tttggaaaca
     4861 gttcgcactt tcggttttga ttttcgctat cagtcaatta atcgtaattt tatggcaagt
     4921 gattgatagt gttactatta ttaagtcact tcaagcgata cgcgtgccat tcgatgttgc
     4981 cataactgaa aaaggagtct atgaccgtgg tgcatcattt attcagatgg gattgattgt
     5041 aactacaaca tttagttttg cgctcattcc tctgttaagt gacgcaatca aaatgaataa
     5101 tcaggtactt atgaatcgtt atgcaaatgc gtcattaaag attacgattt taataagtac
     5161 agcagcggga ataggattaa ttaatttatt gcctttaatg aacggtgtgt tttttaagac
     5221 gaatgattta accttaacgt taagtgttta tatgattacg gtcatttgtg tatcgttaat
     5281 tatgatggat atggcattat tacaagcgca acatgctgtg agacctattt ttgttggtat
     5341 gacggcagga ttggttatta aatttatact taatatcatt ttgattcgtt taagtggcat
     5401 tattggtgcg agcattagta ctgttgtatc attaattata ttcggtacga ttatccatat
     5461 tgctgtcacg agaaaatacc acttacatgc gatgagacga ttttttatca atgttgtttt
     5521 aggtatggta tttatgtcga ttgttgttca atgcgtgtta aacatagtga caacacacgg
     5581 tagaatcact ggactcattg aattattatg tgcagcagta ttaggtatca ttgcattgtt
     5641 tttctatatt tttagattta atgttttgac atataaagag ttaacttatt taccatttgg
     5701 ttcaaagttg tatcaaatta agaaaggaag acgttgatgg cacataccat tacgattgtt
     5761 ggcttaggaa actatggcat tgatgatttg ccgctaggga tatataaatt tttaaagaca
     5821 caagataaag tttatgcaag aacgttagat catccagtta tagaatcatt gcaagatgaa
     5881 ttaacatttc agagttttga ccatgtttat gaagcacatg accaatttga agatgtctat
     5941 aatgatattg tggcgcaatt ggttgaagct gctaatgaaa aagatattgt ctatgcggtt
     6001 ccgggtcatc ctagagttgc tgagacaact acagtgaaat tactggcttt agcaaaggac
     6061 aatactgata tagatgtgaa agttttaggt ggtaaaagct ttattgatga tgtgtttgaa
     6121 gcagttaatg tagatccaaa tgatggcttc acactgttag atgcgacatc attacaagaa
     6181 gtaacactta atgttagaac gcatacattg attacgcaag tttatagtgc aatggttgct
     6241 gctaatttga aaatcacttt aatggaacga tatcctgatg attaccctgt tcaaattgtc
     6301 actggtgcac gaagcgatgg tgcggataac gttgtgacat gcccattata tgaattggat
     6361 catgatgaaa atgcattcaa taatttgacg agtgtattcg taccaaaaat cataacatcg
     6421 acatatttgt atcatgactt tgattttgca acggaagtga ttgatacttt agttgatgaa
     6481 gataaaggtt gtccatggga taaagtgcaa acgcatgaaa cgctaaagcg ttatttactt
     6541 gaagaaacat ttgaattgtt cgaagctatt gacaatgaag atgattggca tatgattgaa
     6601 gaactaggag atattttatt acaagtgtta ttgcatacta gtattggtaa aaaagaaggg
     6661 tatatcgaca ttaaagaagt gattacaagt cttaatgcta aaatgattcg tagacaccca
     6721 cacatatttg gtgatgccaa tgctgaaact atcgatgact taaaagaaat ttggtctaag
     6781 gcgaaagatg ctgaaggtaa acagccaaga gttaaatttg aaaaagtatt tgcagagcat
     6841 tttttaaatt tatatgagaa gacgaaggat aagtcatttg atgaggccgc gttaaagcag
     6901 tggctagaaa aaggggagag taatacatga gattagataa atatttaaaa gtatcacggt
     6961 taataaagcg acgtacgcta gcaaaagaag taagtgatca aggtagaatt acaataaatg
     7021 gtaatgttgc taaagctgga tcggatgtta aagttgaaga tgtgctgacg attcgctttg
     7081 gtcaaaaatt agtaacagtt aaagtaactg cattaaatga acatgcatct aaagataacg
     7141 cgaagggcat gtatgaaatc gttgaagagc gtcgacttga agaagcgtaa attggaggtg
     7201 acaagcaatg aaaaataaag tagaacatat agaaaatcag tacacgtcgc aagagaacaa
     7261 gaaaaaacaa cgtcaaaaaa tgaaaatgcg tgttgttcgt aggcgtatta cagtatttgc
     7321 gggcgtatta cttgcgataa ttgttgtttt atcaatcttg cttgttgtcc aaaaacatcg
     7381 caatgatatc gatgcacagg agcgaaaagc gaaagaagca cagtttcaaa agcaacaaaa
     7441 tgaagaaatt gcgttaaaag aaaagttgaa taatctgaat gacaaagatt atattgaaaa
     7501 aattgcgcgt gatgattatt acttaagcaa caaaggtgaa gtgattttta ggttgccaga
     7561 agacaaagat tcgtctagct caaaatcttc gaaaaaataa atccaaattg attcaaaatt
     7621 atccgagtat agacattgtg aaaaaatcca aacaaggata taataaggga aaatcgaatc
     7681 aaatcgggag gatttattta acatatgtca atcgaagttg gaaataagct taaaggtaaa
     7741 gtcactggta ttaaaaagtt tggtgcattc gtagaattac ctgaaggaaa aagtggttta
     7801 gttcacatta gtgaagtcgc agataattat gttgaaaacg tagaagagca cctttctgtt
     7861 ggtgatgaag tagacgtaaa agtattatct attgctgatg atggaaaaat tagtctttca
     7921 attaagaaag ctaaagaccg tccacgtaga caacatacga gtaaaccaag tcatcaaaaa
     7981 ccagtgcaaa aagccgaaga ttttgaaaag aaattaagca atttcttaaa agatagtgaa
     8041 gataaattaa cttcaatcaa acgtcaaaca gaatctagac gcggtggcaa aggttcaaga
     8101 cgttaattaa aataaataaa gactgtttcg ataaggaata tatttagaat gatgcgtatc
     8161 gaataatcga ttgcagcgtt agacaatcta agactgtttc ttaaataagg agcagtctct
     8221 tttatttgta atgatataac taagacttat accatttttg aaaattgtaa aagtgaggtg
     8281 atgttatgca gttaaatagt aatggttggc atgttgatga ccatattgtt gtcgctgttt
     8341 ctacaggtat tgatagtatg tgtttattgt atcaactact aaatgattat aaagatagtt
     8401 atagaaaact aacatgctta catgtcaatc atggcgttag gtcagcttca atcgaggaag
     8461 ccagattttt agaagtatac tgcgaacgtc atcacatcga tttacatatc aaaaagttag
     8521 atttgtcgca tagtctcgac cgaaataaca gcattcagaa tgaagctcga attaaacgtt
     8581 acgaatggtt tgatgaaatg atgaatgtat tagaagcgga tgtattgcta acggcgcatc
     8641 atttggacga tcaattagaa actattatgt atcgtatttt taatgggaaa tcaacacgta
     8701 ataaactagg atttgatgag ttatcgaagc gaaaaggtta tcagatttat cgaccacttt
     8761 tagctgtctc taaaaaagaa ataaaacaat tccaagagag atatcatatt ccatattttg
     8821 aagatgaatc taataaagat aacaaatatg ttagaaatga tattcgtaat agaattattc
     8881 cagctattga tgaaaataat caacttaaag tatcgcattt attaaaatta aaacaatggc
     8941 atgatgaaca atatgatatt ttgcaatatt cagctaaaca atttattcaa gaatttgtga
     9001 agtttgatga acagtcaaaa tatttagagg tttctagaca agcttttaat aacttaccaa
     9061 actcattaaa gatggttgtg ttggattgcc tattatcaaa gtattatgag ttgtttaata
     9121 ttagtgctaa aacatacgaa gagtggttta aacaatttag tagtaagaaa gcacaattca
     9181 gtattaatct cacggataaa tggataattc aaatcgcata tggtaaatta ataataatgg
     9241 ctaaaaataa tggcgataca tattttagag ttcaaactat tgaaaagcca ggtaattata
     9301 tttttaacaa atatcgatta gagatacatt ctaatttacc aaaatgttta tttccgctta
     9361 cagtgagaac acgacaaagt ggcgatacat ttaaactgaa tgggcgcgat ggttataaga
     9421 aagtgaatcg cctgtttata gattgtaaag tgcaacagtg ggttcgggat caaatgccaa
     9481 ttgtattgga taaacaacag cgcattattg cggtaggaga tttatatcaa caacaaacaa
     9541 taaaacaatg gattataatt agtaaaaatg gagatgaata gcgttatgca taatgatttg
     9601 aaagaagtat tgttaactga agaagatatt caaaatatct gtaaggaatt gggagcacaa
     9661 ttaacaaagg attatcaagg taaaccatta gtatgcgtgg gtatcttaaa aggctcagca
     9721 atgtttatgt cagatttaat taaacgaatt gatacccatt tatcaattga tttcatggat
     9781 gtttctagtt atcacggagg cactgagtca actggtgaag ttcaaatcat taaagattta
     9841 ggttcttcta ttgaaaataa agacgtatta attattgaag atatcttaga gactggtact
     9901 acacttaagt caattactga attattacaa tctagaaaag ttaattcatt agaaatagtt
     9961 actttattag ataaaccaaa ccgtcgtaaa gcggacattg aagctaagta tgtaggtaaa
    10021 aaaataccag atgaatttgt tgttggttac ggtttagatt atcgtgaatt ataccgaaac
    10081 ttaccatata tcggtacgtt aaaacctgaa gtgtattcaa attaattttt taatcaattt
    10141 cagttattat tactatgcgt ttgagaaata atagtgtaga ctcaaaaata tgaaaaatgt
    10201 atttcatata tatttaattt tagacaagac atatgtcttg aaaagttgaa aaatatagag
    10261 attgataaaa ctaatacgga tgtgaatgac attgatgtta agctcaatta ctagcttata
    10321 aaacatgtca tatgttacaa tttttgttag ttttattatg ggaagtagga ggaaatgacg
    10381 catgcagaaa gcttttcgca atgtgctagt tatcgtaata ataggcgtta ttatttttgg
    10441 tctattttca tatttaaacg gtaatggaaa tatgccgaaa cagcttacat ataatcaatt
    10501 tactgagaag ttggaaaaag gtgaccttaa aactttagaa atccaaccac aacaaaatgt
    10561 ctatatggta agtggtaaaa cgaaaaatga tgaagactat tcatcaacta ttttatataa
    10621 caacgaaaaa gaattacaaa aaattactga tgctgctaaa aagcaaaacg gtgtaaaatt
    10681 aacgattaaa gaagaagaaa aacaaagtgt ctttgtgagt atactttcaa cattaattcc
    10741 agttgtagtc atagcgttat tatttatttt cttcctaagc caagcacaag gtggcggtag
    10801 tggcggtcgt atgatgaact ttggtaaatc taaagcaaaa atgtacgata ataataaacg
    10861 tcgtgttcgt ttctctgatg tagcaggggc agatgaagaa aaacaagaat taattgaaat
    10921 tgttgatttc ttgaaagata ataaaaaatt caaagaaatg ggatctagga ttcctaaagg
    10981 tgtcttactt gttggacctc caggtactgg taaaacatta cttgctagag cggttgcagg
    11041 tgaagctggc gcaccattct tctctattag tggttcagac tttgtagaga tgtttgttgg
    11101 tgttggtgcg agccgtgttc gtgacttatt cgataatgct aagaaaaacg cgccttgtat
    11161 tatctttatc gatgagattg atgctgttgg tcgtcaacgt ggtgcaggtg ttggtggcgg
    11221 tcatgatgaa cgtgaacaaa ccctaaacca attattagtt gaaatggatg gtttcggtga
    11281 aaatgaaggt atcattatga tagctgctac aaaccgtcct gatatccttg acccagcctt
    11341 attacgtcca ggtcgttttg atagacaaat tcaagttggt cgtccagatg tgaaaggccg
    11401 tgaagcaatt cttcatgttc atgctaaaaa caaaccactt gatgaaacgg ttgatttaaa
    11461 agcaatttca caacgtacac ctggtttctc aggtgctgat ttagagaact tattaaatga
    11521 agcatcttta attgctgtac gtgaaggtaa aaagaaaatt gacatgagag atatcgaaga
    11581 ggcaacggat agagttatag ccggacctgc taagaaatct cgagttattt ctaagaaaga
    11641 acgtaatatt gttgctcatc acgaagctgg tcatacaatt atcggtatgg tacttgatga
    11701 ggcagaagta gtgcataaag ttactattgt tccacgtgga caagcaggtg gttatgcaat
    11761 gatgctacct aaacaagatc gtttcttaat gactgaacaa gagttattag ataaaatctg
    11821 tggtttactt ggtggacgtg tatcagaaga tattaacttt aacgaagtat caacaggtgc
    11881 ttcaaatgac ttcgaacgtg caacacaaat cgcacgctca atggttacgc aatatggtat
    11941 gagtaaaaaa ttaggaccat tacagttcgg tcatagcaat ggtcaagtat tcttaggtaa
    12001 agatatgcaa ggtgagccta attattcaag ccaaatcgca tatgaaattg ataaagaagt
    12061 tcaacgaatc gttaaagaac aatacgaacg ttgtaaacaa attttattag agcacaaaga
    12121 acaattaatt ttaattgctg aaacattatt aacagaagaa acattagttg ctgaacaaat
    12181 tcaatcatta ttctacgaag gtaaattacc tgaaattgat tatgatgcag ctaaagttgt
    12241 taaagatgaa gattctgaat ttaatgatgg taaattcggt aaatcttatg aagagattcg
    12301 taaagagcaa ttagaagatg gacaacgtga cgaaagtgaa gatcgtaaag aagaaaaaga
    12361 tattgctgag gataaaaaag aagctgataa atctgatgaa aaagatgaac cagcacatcg
    12421 acaagcccca aatatcgaaa aaccttacga tccaaatcac ccagacaata aataatcgat
    12481 tatatacagt acctctttct atgataaagt tatagaaagg ggtactttta tcgtttttga
    12541 aaatacgtat tagattttaa gtcgttgaat tgttatagca gaaaataatt gtaaaacaag
    12601 ttacttcatt atttagaatg atgggtgtag aataagaaca attgttgcat tttatgaagt
    12661 aaagtaattt tttaaatata gagtaataga ggagattgaa ataatgacac acgattatat
    12721 tgttaaagca ttagcatttg atggagagat tagggcttat gctgctttga caactgaaac
    12781 tgttcaagaa gcacaaacga gacattatac atggccgaca gcatctgctg caatgggaag
    12841 aacaatgaca gcaacagcta tgatgggcgc aatgttgaaa ggtgatcaaa aattaactgt
    12901 cactgtagat ggccaaggac ctattggacg aattattgcc gatgcaaatg ctaaaggcga
    12961 ggtgcgtgct tatgtagacc atccacaaac tcattttcca ttaaatgagc aaggtaaact
    13021 tgatgtaaga cgagcagtag ggacaaatgg atctattatg gttgttaaag acgttggaat
    13081 gaaagactat ttctctggag caagtccaat tgtttcagga gaacttggtg aagattttac
    13141 ttattattat gctacaagtg aacaaacacc ttcatcggta ggtcttggtg tattggtaaa
    13201 tcctgataat acgattaaag cagcaggagg atttatcatt caagttatgc caggtgccaa
    13261 agatgaaaca atttcaaaat tagaaaaagc aattagtgaa atgacaccag tttctaaatt
    13321 aattgaacaa ggattaacgc cagaaggatt actaaacgaa atcttaggtg aagaccatgt
    13381 gcaaatttta gagaaaatgc ctgttcaatt tgaatgtaat tgtagtcatg agaaattttt
    13441 aaatgctatt aaaggattgg gcgaggctga gattcaaaat atgattaaag aagatcatgg
    13501 tgctgaagca gtatgtcatt tctgtggaaa taaatataaa tatactgaag aagaattaaa
    13561 cgtgttgcta gaaagtttag cgtaatttaa tttaaatcaa tacgctaaaa tgtttatttt
    13621 tagcggttta gtgaaatgta gaactaaata gttgtataat ccttagtaat tttgtttgct
    13681 ttctagaatt tatttgataa aataattcta tatccgataa ataaactaag atttcaacaa
    13741 ctaactaaaa aggagtgttc ttaatggcac aaaaaccagt agataatatt actcaaatta
    13801 ttggcgatac accggtagtc aaattgagaa atgtagtaga tgacaatgca gcagatgttt
    13861 atgtaaaatt ggaatatcaa aatccaggtg gttctgtaaa ggatagaatt gctttagcaa
    13921 tgattgaaaa agcagagcga gaaggcaaaa ttaaacctgg cgatacaatt gtagaaccaa
    13981 ctagtggtaa tacgggtatc ggtttagcat ttgtatgtgc tgctaaagga tataaagcag
    14041 tatttactat gcctgaaaca atgagccaag agcgtcgtaa tttattaaaa gcatacggtg
    14101 cggaattagt tttaacgcct ggatcagaag cgatgaaagg tgcaattaaa aaagctaaag
    14161 aattaaaaga agaacatggt tacttcgagc cacaacaatt tgaaaaccct gcgaaccctg
    14221 aagttcatga gttaactaca ggtcctgagt tattacaaca atttgaaggg aaaactatcg
    14281 atgcgttcct agctggtgtt ggtactggtg gtacgttatc tggtgtaggt aaagttctga
    14341 aaaaagaata tcctaacatc gaaattgttg ctatagagcc tgaggcttct ccagtattga
    14401 gcggtggtga gccaggtcca cataaattac aaggtttagg tgctggattt attccaggca
    14461 ctttgaatac agaaatctat gacagtatta ttaaagtagg aaatgataca gcgatggaaa
    14521 tgtctcgtcg agttgctaaa gaggaaggta ttttagcagg tatttcatca ggtgctgcga
    14581 tttatgctgc cattcaaaaa gcaaaagaat taggaaaagg taaaacagta gtaacagtat
    14641 tgccgagtaa tggtgaacgc tacttatcaa cacctttata ttcattcgat gactaattaa
    14701 tgtcatttaa aagagtgagt tatctttttg agataacttg ctcttttttt ctaccatgta
    14761 taattttaaa aatatgagcg ttaaattaaa catttttctg ataaaaacat ccagtgaatg
    14821 ataagataat aaacgtacat actaataact agtaaatcgc aggagtaaat tttattagag
    14881 ttaaacaata cataattaaa gggtggttaa catgactaaa acaaaaatta tgggcatatt
    14941 aaacgtcaca cctgattcat tctcagatgg tggaaaattt aataatgttg aatcagcagt
    15001 aactagagtg aaagcgatga tggatgaagg tgctgacatt atagatgttg gaggtgtttc
    15061 aacgagaccc ggccatgaaa tggtttcatt agaagaagag ctgaacagag tattacctgt
    15121 tgttgaagct attgtcggtt ttgatgtgaa aatttcagtc gatacatttc gaagtgaggt
    15181 tgctgaagca tgtttaaaat taggcgttga tatcattaat gatcaatggg cggggctgta
    15241 tgatcatcat atgttccaaa ttgtagctaa atatgacgcg gaaattattt taatgcataa
    15301 tggaaatggt aatcgtgatg aaccggttgt cgaagaaatg ttaacatctt tgttagcaca
    15361 agcacatcaa gctaaaatag ctggtatacc ttcaaataaa atttggctag atccaggtat
    15421 aggtttcgct aaaactagaa atgaagaagc cgaagttatg gcaagactgg atgaacttgt
    15481 tgcaacagaa tatccagttt tattagcgac aagccggaaa cgtttcacta aagagatgat
    15541 gggttatgat acaacaccgg ttgaaagaga tgaagtaact gcagctacga ctgcatatgg
    15601 tattatgaaa ggcgttagag cagtacgcgt tcataatgtc gagttgaatg ctaaattagc
    15661 taaaggtata gattttttaa aggagaatga aaatgcaaga cacaatcttt cttaaaggta
    15721 tgcgctttta tggatatcat ggtgctttat cagctgaaaa tgaaataggg caaattttca
    15781 aagtggatgt aactttgaaa gtagacttag ctgaagctgg gcgtactgat aatgttattg
    15841 atacagttca ttatggtgaa gtgttcgaag aggttaaatc aattatggaa ggtaaggccg
    15901 ttaatttact tgagcatcta gctgaacgta ttgcaaatcg tataaattca caatataatc
    15961 gtgtaatgga aacgaaagtg agaatcacta aagaaaaccc accgattccg ggtcattatg
    16021 atggagtagg tatcgaaata gtgagggaga ataaatgatt caagcatact taggattagg
    16081 tagtaatatt ggtgatagag aaagccagtt aaacgatgct ataaagattt tgaatgaata
    16141 tgatggtatt aacgtatcta atatttctcc gatttatgaa acagcaccag ttgggtatac
    16201 tgagcaacct aactttttaa atttgtgtgt tgaaattcaa acaacactca cagtattaca
    16261 actgttggaa tgttgtttga agacagaaga atgtttacac cgtgttagaa aggaacgatg
    16321 gggtcctaga actttagatg tggatatttt gttgtatgga gaagaaatga tagatttacc
    16381 aaaactgtcg gtgccacatc cgagaatgaa tgaacgtgca tttgttttaa tcccattaaa
    16441 tgatatagca gcaaatgtca tagaaccacg ttcgaaattg aaagtgaaag atttagtttt
    16501 tgtcgatgac agtgtaaaga gatataaata atgtattgtt gagaacattc atatttattg
    16561 ggaatagatg attgattatt ggagatgttt ccgcattttg attatctcta aatgcatttg
    16621 atttcgaatt agtatatgat gattcagtat gtgttagaat taagttattt cataaaatca
    16681 aagtaagcaa tatttgataa attgctgtaa tgtatttggg cttttgttga agtttgtgga
    16741 agataatttt tatacattta cttagggttg tattaatgat aatcgtattt taaatgaata
    16801 agtaattcat aaacgaaaac agaaattgct ttattacatt tacaacctct tgtaaaatta
    16861 aaaaagtgat aaaattgatg ttatgaatat aagaaaacat gccacatcat aactttaagg
    16921 tgtaatggtt aatgataaag tattagaaac atcgaaagag atgtatgttg agcaaaaatg
    16981 tctgatattt tataaaactt taaaggaaaa tgtttgagtg taccagttgg aatactaaag
    17041 gattacaaca agttaaagga gagaaagtta tgtcagaaga aatgaatgac caaatgttgg
    17101 ttcgacgtca aaaattacaa gaattatatg atcttggtat agacccgttt ggttctaaat
    17161 ttgaccgttc aggtttatct agtgatttga aagaagagtg ggaccagtat tctaaagaag
    17221 aattggtaga aaaagaagcg gatagtcatg tcgctatagc tggacgatta atgactaagc
    17281 gtggtaaagg taaagcagga tttgcacacg ttcaggactt agctggacaa attcaaattt
    17341 acgttcgtaa agatcaagtt ggcgatgacg aatttgattt atggaaaaat gctgatttag
    17401 gcgatatcgt tggtgttgaa ggtgtaatgt tcaaaacaaa tactggcgaa ttatcggtta
    17461 aagcgaagaa attcacgcta ctaactaaat cattgcgacc attaccagat aaattccacg
    17521 gtttacagga tattgaacag agatatcgtc aaagatattt agatttaatt acgaacgaag
    17581 atagcactcg tacatttatt aatcgtagta aaatcattca agaaatgcgt aattatttaa
    17641 ataataaagg tttcttggaa gtagaaacac ctatgatgca ccaaattgct ggtggcgcag
    17701 ctgctagacc atttgtaaca catcataatg cattagatgc aacgttatac atgcgtattg
    17761 ctattgagtt gcatttaaaa cgtttaattg tcggtggact tgaaaaagta tatgaaattg
    17821 gtagagtatt ccgtaatgaa ggtgtatcaa ctagacataa ccctgaattc acaatgattg
    17881 aattatatga agcatatgca gattatcatg acattatgga tttaacagaa tctatggtga
    17941 gacatattac caatgaagta ttaggttctg caaaagtaca atacaatggg gaaacgattg
    18001 atttagaatc tgcttggact cgtttgcata ttgttgatgc tgtaaaagaa gctactggtg
    18061 tagattttta tgaagttaaa agtgatgaag aagctaaagc tttagctaaa gaacatggta
    18121 ttgaaattaa agatacaatg aaatatggtc atattttaaa tgaattcttt gagcaaaaag
    18181 ttgaagaaac acttattcag ccaacgttta tctatggtca tccgactgaa atttcacctt
    18241 tagcgaagaa aaatcctgaa gatcctagat ttactgatcg tttcgaattg ttcattgtag
    18301 gtagagagca tgcaaatgca tttactgaat taaatgatcc tattgatcaa aaaggtcgtt
    18361 ttgaagcgca acttgttgaa aaagcgcaag gtaatgatga agcgcatgaa atggatgaag
    18421 attacattga agcgttagaa tatggtatgc ctccgacagg tggtcttggt atcggtattg
    18481 atagattggt tatgttatta actgactctc catcaatcag agacgtgtta ttattccctt
    18541 atatgagaca aaaataaatg acgttgattg ttagtaagag ctctcgtgta tacaacatgt
    18601 gtatgcgagg gttttcttaa ttatggtaat tagttcgtgt ttgaatgttt ttgatagtaa
    18661 tagttaacga taatggtgct atttttgact gttaaacaag gtagttggct gatagatgaa
    18721 aatgacgata cgtatataga gtagttcgga tgagaaatgt taacgataga cattgagata
    18781 tcttataaca aaaattgcta tattagtata atttatctta atcagctata aaagtacttt
    18841 aaaattgtat agaatgtgta tggttttgta catatgtgta tgatagaata ctaaaagtgt
    18901 tatgaattag agagcacgag aaatgtcagt tttgaagaat aaaaaagttg attaaaagtg
    18961 ttgactttat caattgaatg aagtaatata taaaagtcgt caaaaacaga cgaaacacac
    19021 taaaagctga tgtgacaaag ttcacaacaa agtgtaaaat taactattgc accttattaa
    19081 ttaagcgtgt atcataaatt agtaagttat tttgtctggt gactatagca aggaggtcac
    19141 acctgttccc atgccgaaca cagaagttaa gctccttagc gtcgatggta gtcgaactta
    19201 cgttccgcta gagtagaacg ttgccaggca aatgacaaat cggagaatta gctcagctgg
    19261 gagagcatct gccttacaag cagagggtcg gcggttcgaa cccgtcattc tccaccattt
    19321 attcttagat atagccggcc tagctcaatt ggtagagcaa ctgacttgta atcagtaggt
    19381 tgggggttca agtcctctgg ccggcaccat cttttgagcc attagctcag ctggtagagc
    19441 atctgacttt taatcagagg gtcagaggtt cgaatcctct atggctcatt acgatttaat
    19501 ttttatattt agcaaaataa tgcagaagta gttcagcggt agaatacaac cttgccaagg
    19561 ttggggtcgc gggttcgaat cccgtcttct gctccattat tttgccgggg tggcggaact
    19621 ggcagacgca caggacttaa aatcctgcgg tgagagatca ccgtaccggt tcgattccgg
    19681 tcctcggcac cattttagcg cccgtagctc aattggatag agcgtttgac tacggatcaa
    19741 gaggttatgg gttcgactcc tatcgggcgc gccattttta aattaattga ataacgggaa
    19801 gtagctcagc ttggtagagc acttggtttg ggaccaaggg gtcgcaggtt cgaatcctgt
    19861 cttcccgatt acttcttaaa ttccatttta tgggggctta gctcagctgg gagagcgcct
    19921 gctttgcacg caggaggtca gcggttcgat cccgctagtc tccaccattt attttttaca
    19981 cgatgaacat tgaaaactga atgacaatat gtcaacgtta attcc
//
LOCUS       DAHHOJ010000045        19998 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.045, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000045 DAHHOJ010000000
VERSION     DAHHOJ010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19998)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19998)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19998
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.045"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..328)
                     /locus_tag="NBH32_001376"
     CDS             complement(<1..328)
                     /locus_tag="NBH32_001376"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514981.1"
                     /translation="MNDQNSFRTYLIIDKSDLEIMGYFCLKIINIKLEKDVSGKTKRK
                     ISSDAKKNGEFQALLITKLDRSDKHKKHIPGEIVMDYALSIASEIYDRTALRHICVDW
                     YDNKNLK"
     gene            complement(474..626)
                     /locus_tag="NBH32_001377"
     CDS             complement(474..626)
                     /locus_tag="NBH32_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1514982.1"
                     /translation="MVPEEKGSITLSKEAAIIFATAKFKPFKNRIKNNPLKTNPFLKL
                     HENKKS"
     gene            complement(1155..1514)
                     /locus_tag="NBH32_001378"
     CDS             complement(1155..1514)
                     /locus_tag="NBH32_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 domain-containing protein"
                     /protein_id="HCD1514983.1"
                     /translation="MNIISIILIIFVALEFFYIMYLETIATTSKKTSETFNISVDKLK
                     DKNINVLLKNQGVYNGLIGVLLIYGLFISSNPKEICAAILVYIIGVAIYGGLSSNISI
                     FFKQGTLPVLALISMLW"
     gene            complement(1533..2378)
                     /locus_tag="NBH32_001379"
     CDS             complement(1533..2378)
                     /locus_tag="NBH32_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCD1514984.1"
                     /translation="MNIMLTGATGHLGTHITNQAIANHIDHFHIGVKNVEKVPEDWRG
                     KVSVRQLDYFNQESMVEAFKGMDTVVFIPSIIHPSFKRIPEVENLVYAAKQCGVAHII
                     FIGYYADQHNNPFHMSPYFGYAARLLATSGIDYTYVRMAMYMDPLKPYLPELMNMHKL
                     IYPAGDGRINYITRNDIARGVIAIIKNPDTWGKCYLLSGYSYDMKELAAILSEASGTE
                     IKYEPVSLETFAKMYDEPKGFGALLASMYDAGARGLLDQESNDFQQLVNDQPQTLQSF
                     LQENI"
     gene            2850..3530
                     /locus_tag="NBH32_001380"
     CDS             2850..3530
                     /locus_tag="NBH32_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL1"
                     /protein_id="HCD1514985.1"
                     /translation="MKFKAIAKASLALGMLATGVITSNVQSVQAKAEVKQQSEADLKL
                     YYSGPSFEYKKVTGYGFVEGKDRFIDFIYNGQYNKISLVGSDKDKYNEEVNPDIDVFV
                     VREGNGRQADNHSIGGVTKTNRGVYYDYIHTPILEIKKGKEEPQSSLYQIYKEDISLK
                     ELDYRLRERAIKHHGLYSKGLKQGQITITMNDGTTHTIDLSQKLEKERMGQSVDGKQI
                     QKILVEMK"
     gene            3814..4515
                     /locus_tag="NBH32_001381"
     CDS             3814..4515
                     /locus_tag="NBH32_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL2"
                     /protein_id="HCD1514986.1"
                     /translation="MKMKSIAKVSLVLGILATGVNTVTEQPVHAENKPIQVSQNSKNL
                     KAYYTQPSVEYKNVTGYISSIQPKAGTKFMNIIEGNTVNNLALVGKDKQHYHTGVHRN
                     LDIFYVNEDKRFEGSKYSIGGITEASDKAVDQIAEARVIKEDHTGEYDYDFFPFKIDK
                     EAMTLKEVDFKIRKHLIDHYGLYGEMSSGTVTVKMKYYGKYTFELDKKLQEDRMADII
                     KVTDIDRIEVTVKKA"
     gene            4805..5836
                     /locus_tag="NBH32_001382"
     CDS             4805..5836
                     /locus_tag="NBH32_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000702713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL3"
                     /protein_id="HCD1514987.1"
                     /translation="MKIRTIAKASLALGLVTTGASIVTTQSANAEVASALKAGQLAKI
                     NVSSSAVTTTTQAVNAEQNRTANTEQASKSNTEKVSTSPSIKTEQTPKSNTQKATQPT
                     PSGTTQPLPNAQPQSPQSTPNASTSPSSSVETQQPQSPTTKQAQKEINPKYKDLRTYY
                     TKPSLEFEKQFGFMLKPWTTVRFMNVIPDWFIYKIALVGKDDKKYKDGPYDNIDVFIV
                     LEDNKYQLKKYSVGGITKTNSKKVDHKARISITKEDEKGKISRDDSEYKITKEEISLK
                     ELDFKLRKQLIEQHNLYGNIGSGTIVIKTKNGGKYTFELHKKLQEHRMADVIDGTSID
                     RIEVNLKSS"
     gene            6197..6901
                     /locus_tag="NBH32_001383"
     CDS             6197..6901
                     /locus_tag="NBH32_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL5"
                     /protein_id="HCD1514988.1"
                     /translation="MKIRTIVKASLALSILATGVITSTAQTVNASEHEAKYENVTKDI
                     FDLRDYYSGESKALKNVIGYHYSKGGRHYLVIDKNRKFTRVQIFGKDIERFKARKNPG
                     LDIFVVKEAENRNGTVYSYGGVTKKNQGAYYDYINAPRFQIKRDEGDGIATYGRVHYI
                     YKEEVSLKELDFKLRQYLIQNFDLYKKFPKDSKIKVIMKDGGYYTFELNKKLQTNRMS
                     DVIDGRNIEKIEANIR"
     gene            7349..8044
                     /locus_tag="NBH32_001384"
     CDS             7349..8044
                     /locus_tag="NBH32_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL7"
                     /protein_id="HCD1514989.1"
                     /translation="MKLKTLAKATLALGLLTTGVITSEGQAVKAAEKQERVQHLHDIR
                     DLHRYYSAPSFEYSNISGKVENYNGSNVVRFNQKDQNHQLFLLGKDKEEYKEGLQGQN
                     VFVVQELIDPNGRLSTVGGVTKNNNKTSETKTQLVVNKVDGGDLHASIDSFLIQKEEI
                     SLKELDFKIRQQLVNNYGLYKGTSKYGKIIINLKDENKVEIDLSDKLQFERMGDVLNS
                     KDIRGIAVTINQI"
     gene            8385..9083
                     /locus_tag="NBH32_001385"
     CDS             8385..9083
                     /locus_tag="NBH32_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL9"
                     /protein_id="HCD1514990.1"
                     /translation="MKLTALAKATLALGILTTGVFTAESKAVHAKVELDETQRKYYIY
                     MLKDYYSEESYEPTNISVKSEDYYGSNVLNFNHRNKNFKVFLLGDDKNKYKEKTHGLD
                     VFAVPELIDTKGGIYSVGGITKKNVRPVFGCVSHPGLLVKKIDPKHGFSVNELFFIQK
                     EEVSLKELDFKIRKMLVEKYRLYKGASDKGRIVINMKDEKKYVIDLSEKLSFDRMFDV
                     MDSKQIKNIEVNLN"
     gene            9450..10133
                     /locus_tag="NBH32_001386"
     CDS             9450..10133
                     /locus_tag="NBH32_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL10"
                     /protein_id="HCD1514991.1"
                     /translation="MKLTALAKATLALGILTTGTLTTEAYSGHAKQNQKSVNKHDKEA
                     LHRYYSGNFKEMKNINALRHGKNNLRFKYRGMKTQVLLPGDEYRKYQQRRHTGLDVFF
                     VQEKRDKHDISYTVGGVTKSNKTPGFVSAPLLNVTKENGEDAFVKGYPYDIKKEEISL
                     KELDFKLRKHLIEKYGLYKTKSKDGKIKINLKDGSFYNLDLRSKLKFKHMGEVIDSKQ
                     IKDIEVNLK"
     gene            10392..11948
                     /locus_tag="NBH32_001387"
     CDS             10392..11948
                     /locus_tag="NBH32_001387"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000028656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HCD1514992.1"
                     /translation="MSITEKQRQQQAELHKKLWSIANDLRGNMDASEFRNYILGLIFY
                     RFLSEKAEQEYADALSGEDITYQEAWADEEYREDLKAELIDQVGYFIEPQDLFSAMIR
                     EIETQDFDIEHLATAIRKVETSTLGEESENDFIGLFSDMDLSSTRLGNNVKERTALIS
                     KVMVNLDDLPFVHSDMEIDMLGDAYEFLIGRFAATAGKKAGEFYTPQQVSKILAKIVT
                     DGKDKLRHVYDPTCGSGSLLLRVGKETQVYRYFGQERNNTTYNLARMNMLLHDVRYEN
                     FDIRNDDTLENPAFLGHTFDAVIANPPYSAKWTADSKFENDERFSGYGKLAPKSKADF
                     AFIQHMVHYLDDEGTMAVVLPHGVLFRGAAEGVIRRYLIEEKNYLEAVIGLPANIFYG
                     TSIPTCILVFKKCRQQDDNVLFIDASNDFEKGKNQNHLSDAQVDRIIATYKRKETIDK
                     YSYSATLQEIADNDYNLNIPRYVDTFEEEEPIDLDQVQQDLKNIDKEIAEIEQEINAY
                     LKELGVLKDE"
     gene            11941..13200
                     /locus_tag="NBH32_001388"
     CDS             11941..13200
                     /locus_tag="NBH32_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1514993.1"
                     /translation="MSNTQKKNVPELRFPGFEGEWEEKKLGDLTDRVIRKNKNLESKK
                     PLTISGQLGLIDQTEYFSKSVSSKNLENYTLIKNGEFAYNKSYSNGYPLGAIKRLTRY
                     DSGVLSSLYICFSIKSEMSKDFMEAYFDSTHWYREVSGIAVEGARNHGLLNVSVNDFF
                     TILIKYPSLEEQQKIGKFFSKLDRQIELEEQKLELLQQQKKGYMQKIFSQELRFKNEN
                     GNDYPDWERIKFFDVIDKVIDFRGRTPKKLNMEWSDEGYLALSAVNVKKGYIDFNVEA
                     KYGNLDLYTRWMRGNELYKGQVLFTTEAPMGNVAQVPDNKGYILSQRTIAFNSNEKIT
                     DNFLASLLSSENVYNDLLKLCSGATAKGVSQKNLNRLYVTIPHSISEQEEIAEFFRKI
                     NQLVELQKYKIEHTKSQKQVFLQKMFI"
     gene            13613..14299
                     /locus_tag="NBH32_001389"
     CDS             13613..14299
                     /locus_tag="NBH32_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL11"
                     /protein_id="HCD1514994.1"
                     /translation="MKLKNIAKASLALGILTTGMITTTAQPVKASTLEVRSQATQDLK
                     DYYKGQSFDLKNIGGYKEGNKVTFIYHNQQIDVTLTGNEKLEFNDDGEISNVDVFVVR
                     ENSDRSGITSSNGGITTSNKQDIIDNVKSVNMQITKKTSSNTETVTSSYYSISKENIS
                     LKELDFKLRKHLIDKNGLYKTEPKNSKIRVTMEDGGFYTFELNKKLQTNRMGDVIDGT
                     KIKEINVELN"
     gene            14321..15805
                     /locus_tag="NBH32_001390"
     CDS             14321..15805
                     /locus_tag="NBH32_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKLRK protein"
                     /protein_id="HCD1514995.1"
                     /translation="MRENFKLRKMKVGLVSVAITMLYIMTNGQAEASETNQKPSTNQE
                     STAVTHTEQNSKETKTAESSKNFVNLDPIKPGDQKVTGTTMPNHIILLNIDGKSVDSV
                     DGEDSSFITSKEDGKFEYPLNNRKIVHNQEIEVSSSSLDDLGEDDEEVEEDSTEKSEA
                     EEESTGAKVTYTTPRYEKAYEVPEKRLEEKDGHHQVFIEPITEGSGIIKGHTSVKGKV
                     ALSINNKFINFETNSNGGPNKEEAKSGSEGIWMPIDDKGYFNFDFKTKRFDNLELKEG
                     NDISLTFAPDDDEDALKPLIFKTKVTSLEDIDKAETKYDHTKVNKVKILDNVKEDLHV
                     DEIYGSLYHTDKGKGILDKEGTKVIKGKTKFANAVVKVDSELGEAQLFPDLQVNEKGE
                     FSFDSHGAGFRLQNGEKLNFTVVDPITGELLSNEFVSKEIDIEETPEEKADREFDERL
                     ENTPAYYKLYGDKIVGFDTNDFPITWFYPLGEKKVERITPKLEK"
     gene            complement(15912..16220)
                     /gene="spn"
                     /locus_tag="NBH32_001391"
     CDS             complement(15912..16220)
                     /gene="spn"
                     /locus_tag="NBH32_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="myeloperoxidase inhibitor SPIN"
                     /protein_id="HCD1514996.1"
                     /translation="MKFKKVLVATAMVGVLATGVVGYGNQADAKVYSQNGLVLHYDAN
                     FLEHELSYIDVLLDKNADQATKDNLRSYFADKGLHSIKDIINKAKQDGFDVSKYEHVK
                     "
     gene            16587..17369
                     /locus_tag="NBH32_001392"
     CDS             16587..17369
                     /locus_tag="NBH32_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1514997.1"
                     /translation="MGYLKRFTLYISIFILIVVIAGCGKSDETKEDSKEEQIKKSFAK
                     TLDMYPIKNLKDLYDKEGYRDGEFKKGDKGTWTLLTSFSKSNKPGEIDDEGMVLYLNR
                     NTKKATGYYFVNKIYDDISKNQNEKKYRVELKNNKIVLLDNVEDEKLKQKIENFKFFS
                     QYADFKDLKNYQDGSITTNENVPSYEAEYKLNNSDENVKKLRDIYPITTKKAPILKLH
                     IDGDIKGSSVGYKKIEYKFSKVKDQETTLRDYLNFGPSDEDI"
     gene            17417..18196
                     /locus_tag="NBH32_001393"
     CDS             17417..18196
                     /locus_tag="NBH32_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1514998.1"
                     /translation="MGYLKRIGLCISLLILSIFVTSCDSDNKITGDSKEAQIKKSFAK
                     TLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVVRSEMIIQPKGKSLTSRGMILYMNR
                     NTRTTTGYFSIEEIDSRKNLDERETEKKYPVKIINNKIIPTVKIKDEKLKKEIENFKF
                     FVQYGSFKGIENYENGHISYNSEGPIYSARYQLKNDDYNVKELRKRYDIPTEKAPKLL
                     LKGSGDLKGSSVGYKEIEFIFLENKKENIYFSDGLNLIPGD"
     gene            18257..19051
                     /locus_tag="NBH32_001394"
     CDS             18257..19051
                     /locus_tag="NBH32_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1514999.1"
                     /translation="MRDSKRVVLYISIMVLSIFIIGCNSSSENAKKSKEDSKEDQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFEKGDKGTWTIYTDFAKSNKAGELDSEGMVL
                     HLNRNTRTAKGYYFVRKTYNEEDKMLQRKDYRIKLKNNKIVLLDKVEDKKLKHKIENF
                     KFFGQYADFKDLKNYKNGKISSNENVPYYEAEYKIENSDRNVKKLRNVYPITTSESPT
                     LKFHIDGDIKGSSIGYKKVEYIFSKQKDNQISVSDFLNFNPSKDNQ"
     gene            19090..19887
                     /locus_tag="NBH32_001395"
     CDS             19090..19887
                     /locus_tag="NBH32_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1515000.1"
                     /translation="MRNLKRDVLYISIMALSIFIIGCDKSSVTAEKAKEDSKEDQIKK
                     SFAKTLDMYPIKNLEDLYDKEGYRDGEFKKGDKGTWVIRSEMTTELKDENMESKGMVV
                     RLNRNTRTTTGEYFVRIIKEDNEGKVYSDERKYPVKIDNKKIIPLKLIDDEKVKKEIE
                     EFKFFVQYGNFKELENYKDGKVSYNPEVPIYSAQYQLKNSDYNVEQLRKRYNIPTRKA
                     PKLLLKGSGNLKGSSVGYKNIEFTFVENKEENIYFTDSINFNPSEDK"
BASE COUNT         7850 a         2654 c         3574 g         5920 t
ORIGIN      
        1 ctttcaaatt cttattatca taccaatcaa cacaaatatg tcttagtgca gtacgatcat
       61 aaatttcaga agcaatagat aaggcatagt ccattacaat ttctcctggt atatgctttt
      121 tgtgtttatc gctcctatct aatttagtta ttaataatgc ttgaaattct ccattctttt
      181 tagcatcaga cgagattttt cttttcgttt ttccactaac gtctttttca agctttatat
      241 ttataatttt taagcagaag tatcccatta tttcaaggtc tgatttatct ataattaaat
      301 acgtacggaa ggaattttga tcgttcattg ttattgcttt atgttttagg gaatgtgcta
      361 ttgaagggtt taaagatgtt tgaaatgtct tggtttcttg acttatatag ttatgaaaga
      421 gggcgttgtc ctttatagca tttctgagat tagaaatgct ataaataaag ttattaagat
      481 tttttgtttt catgtaattt aagaaatgga tttgttttta gtgggttatt tttaattctg
      541 ttcttaaatg gtttgaattt tgcggttgca aatatgatag cagcttcttt tgacaaagta
      601 atagaacctt tttcttctgg taccatgata ttcactcctt gtacaattgg atttaaagtt
      661 tataaaataa ttttactatg tataataatt tgtgtaaata gtttcttatt gttaccgaat
      721 aatttgtttg gaagcgttat gtaatgccaa tcaacaccaa agtcctcatg tcctcattcc
      781 ttttgcgact acatatattg ttaataatat tgaaattata aactgatagt gataattttg
      841 ttaactgacc catgaaagta ggaaccccac catacgggaa tttcgaaatc atggtttttt
      901 tgttttaaag tattgatgca atagataaag tagccattct aatatatgga gtatttatca
      961 gctaaaaatt aaaacagata tgaattttct tcattaaatc atttttccat atagaaacag
     1021 aggcaacgct actgtcacct ctgcttgaaa taattaggtt gaaatactct gggtctaaag
     1081 tccacaccta aatcacccaa atcgctcaaa ctctcaaccc cagactaaat ctccaaaccc
     1141 caaaacacca atacttacca aagcattgaa atgagtgcca atactggcaa cgtaccttgt
     1201 ttgaaaaaga tactaatatt gcttgaaagg ccaccataaa tagcaacgcc aatgatatac
     1261 actaaaatag ctgcgcatat ttcttttgga ttactgctga taaacaaacc gtatattagc
     1321 aaaactccga ttaaaccatt atatacgcct tgattcttca aaagtacgtt aatatttttg
     1381 tctttcaatt tatcgacgct tatattaaat gtctcgctag tctttttgga agttgtagca
     1441 atcgtttcaa ggtacataat atagaaaaat tctaatgcca caaatatgat taaaattatc
     1501 gagatgatat tcactgtaac gctcctttag tattaaatat tttcttgtaa aaatgattgt
     1561 agtgtctgtg gttgatcatt gactaattgt tggaaatcat tggattcttg gtctaatagt
     1621 cctcttgctc ctgcgtcgta cattgatgcc aataatgcac caaagccttt aggttcatcg
     1681 tacatttttg caaatgtctc taatgaaacg ggttcatatt taatttctgt gcctgatgcc
     1741 tcagataaaa tagcagcaag ttctttcata tcataactgt agcctgataa taaataacat
     1801 ttgccccaag tatctggatt tttaataata gcaatgacgc ctctagcaat atcatttcta
     1861 gtaatataat taatacgacc atcaccagct ggataaatca gtttatgcat attcatcaat
     1921 tctggtaaat atggtttaag tggatccatg tacattgcca ttcttacata ggtatagtca
     1981 atgccacttg ttgccaatag acgtgctgca taaccaaaat aaggactcat atggaatgga
     2041 ttattatgct gatctgcgta ataacctatg aaaatgatat gagcaacgcc acattgcttt
     2101 gcagcatata ctaaattttc cacttcagga atacgtttga atgatggatg gataatactt
     2161 ggaataaaca caacggtatc cattccttta aatgcttcta ccatgctttc ttgattaaaa
     2221 taatctaatt gtcgaacaga aacttttcca cgccaatctt ctggaacttt ctcaacattt
     2281 ttaacaccaa tgtgaaaatg atctatgtga tttgcaatgg cttgatttgt aatatgtgtg
     2341 cctaaatgac ctgtagcacc tgttaacata atattcattc acttcatctc ctaatcttta
     2401 tatacataac ataatactta ttcgatggtt ttcaaaacat ttgattttat aaaaaattgt
     2461 aatctgtatt tattgtcgtc gtgtatagta aatacgtata tgttattaat gttgaaaata
     2521 ccgtaatgac gcgttttagt tgatgtgtgt caccatgatc tttgaaattt ttgacatggt
     2581 actgcgacga tatggtgtca tttttgtgtc taaaagtttt acagttttta aaattaaaat
     2641 ggtataaagt gtgaattgta taaaaaagag tctcgacgga taagaattga ttaataacag
     2701 ttagcatttt attaattacc ttaacaatga ttcaagttta gttaaatgag gtttaatttg
     2761 aaaggggata gcgcctcaat ataatgtagg tagattgttc atattacgta attgaaaaat
     2821 caaatttaaa tagattgggg ctaaaaatta tgaaatttaa agcgatagca aaagcaagtt
     2881 tagcattggg aatgttagca acaggtgtaa ttacatcgaa tgtacaatca gtacaagcga
     2941 aagcagaagt taaacaacaa agtgaggctg atttaaaact ttattatagt ggaccaagtt
     3001 ttgaatataa aaaagtaact ggatatggat ttgttgaagg taaagataga ttcattgatt
     3061 ttatatacaa tggacaatat aataaaatat ctttagttgg ttctgataaa gataaatata
     3121 atgaagaagt taatccagat atagatgtgt ttgtcgttag agaaggaaac ggtagacaag
     3181 ctgataatca ttcgattggt ggcgtaacaa aaactaatcg aggagtgtat tatgactata
     3241 tacacacacc aatccttgaa atcaagaaag gtaaagaaga accacaaagt agtctatacc
     3301 aaatttataa agaagacatt tcgctaaaag aacttgatta tagattaaga gaacgtgcaa
     3361 tcaaacatca cggcttgtat tcaaaaggtc ttaaacaagg tcaaattaca attacaatga
     3421 atgatggcac aacacataca atcgatttaa gtcaaaaact tgaaaaagag cgtatgggtc
     3481 agtcagtaga cggcaaacaa atacaaaaaa ttctagtaga aatgaaataa tactttctaa
     3541 caacaaagca ctacgctgaa tagcacttgt tttggaatat atggaagtta agcgacgtac
     3601 tgttgcttag cttctttttt tgagggtcaa agttacaaaa ctcacacaaa cagtcgcacc
     3661 acgcattatc ttttacttaa ctagcttaat catattttat gaatagttaa aaaagggtta
     3721 atgtgaatat cagaatacag ctcctataat atggttgtat gattcaattt acgtaataaa
     3781 acaatctaat ctaatatatt ggagcataca actatgaaaa tgaaatcaat tgcaaaagta
     3841 agtttagtct tgggtatttt agctacaggt gtaaatactg taacggaaca accggtgcat
     3901 gccgaaaata aacctattca agtaagccaa aatagtaaaa atttaaaagc gtactatact
     3961 caaccgagtg ttgaatataa aaatgtgaca ggttatatca gtagtattca acctaaagca
     4021 ggtactaaat ttatgaatat tatagaaggt aatacagtta ataatctagc tttagttggc
     4081 aaagataagc aacactatca tacgggtgta catcgtaacc ttgatatttt ttatgttaat
     4141 gaggataaga gatttgaagg ttcaaagtac tctattggtg gtatcacaga agcaagcgat
     4201 aaagctgttg accaaatagc agaagcaaga gttattaaag aagatcatac tggtgaatat
     4261 gattatgact ttttcccatt taaaatagat aaagaagcaa tgacattgaa agaggtcgat
     4321 tttaaaataa gaaaacatct tatcgatcat tatggtcttt acggtgaaat gagttcagga
     4381 acagttactg ttaaaatgaa atactatggc aagtatacat ttgaattaga taaaaagtta
     4441 caagaagacc gaatggccga tatcattaaa gtcacagata ttgacagaat tgaagttaca
     4501 gttaaaaaag cataatgctt agactggtcg tcgtaatgaa ttgaaattgc aatagagagg
     4561 ttaagtgatg ataaaacgtt acttaacttc ttttttatgt cttaaaatca tttcaatgat
     4621 acgtagtaac accacataaa cctcgaaagt actcattatt ttttgcttaa atgacttaat
     4681 aatacttcaa caattgttaa acgagggtta atgtgaatag accaatatgt cctctataat
     4741 gaagttgtat gattctaatt acgtaaagaa caatcgaata taattacgat tggagcatac
     4801 aactatgaaa ataagaacaa ttgcgaaagc gagtttagcg ttagggcttg taacaacagg
     4861 tgcaagtata gtaacaacgc aatcggccaa tgcagaagta gcatcagcac ttaaagcagg
     4921 acagttagca aagataaacg tatcatcaag tgcagtcaca acgacaacgc aagcagtgaa
     4981 cgcagaacaa aatcgtacgg ctaatactga acaggcatca aagtctaata cagaaaaagt
     5041 atcaacatcg ccttcaatta aaacagaaca gacgccaaaa tctaacacac aaaaggcaac
     5101 tcaaccaaca ccaagcggaa caactcagcc tttaccaaat gcacaaccgc aatcaccaca
     5161 atcaacacca aacgcatcaa catcgccttc atctagtgta gaaacacaac aaccgcaatc
     5221 gcctaccaca aaacaagcac aaaaggaaat aaaccctaaa tataaggatt taagaacata
     5281 ttatacgaaa ccgagtttag aattcgaaaa gcaatttggt tttatgctga aaccatggac
     5341 gacagttagg tttatgaatg ttataccaga ttggttcatt tataaaatag ctttagttgg
     5401 aaaagacgat aagaaatata aagatgggcc atacgataat attgatgtat ttatcgtttt
     5461 agaggacaat aaatatcaat taaaaaaata ttctgtcggg ggtatcacga agacgaatag
     5521 taaaaaagtt gatcacaaag caagaatcag tattactaaa gaagatgaga aaggcaaaat
     5581 ctcacgcgat gattcagaat acaagattac taaagaagag atttccttga aagagcttga
     5641 ttttaaattg agaaagcaac tcattgaaca acataatctg tacggtaata ttggttcagg
     5701 aacaattgtt attaaaacga aaaatggtgg gaagtacacg tttgaattac acaaaaaatt
     5761 acaagaacat cgcatggcag atgtcataga tggcactagt atcgacagaa ttgaagtgaa
     5821 tctaaaatca tcgtgatgga tactgcatag aacctatgaa cgatgtgccg agaagttgag
     5881 tgacaactat ttacatgttg ctcagcttct ttcgtgttgc gcagtgatat aagaagacga
     5941 atatttgatg ttgaccacac ataacatact tatgtcctaa atgtgtctaa aaatcccaat
     6001 ctatcaagcg ttgaagtaca acaaaagtca tttttattta acgaacatta tggattcctt
     6061 aatttaatta acgatgattc aaatttagtt aaacaaggtt taatgtgaat agagtcatac
     6121 gccaactata ataaagctgt gtgattcaat aaacgtaatc gaacaagtct aataattacg
     6181 attggagcat acaactatga aaataagaac aattgtgaaa gccagtttag ctctaagtat
     6241 tttagcaact ggggttataa catcaacggc tcaaactgta aatgcgagtg aacatgaagc
     6301 aaaatatgaa aatgtgacaa aagatatctt tgacttaaga gactactata gtggtgaaag
     6361 taaagcgctt aaaaatgtta tcggttatca ctatagtaaa ggcggtagac actaccttgt
     6421 cattgataaa aatagaaagt tcacaagagt acagatattt ggtaaagata ttgaaagatt
     6481 taaagcacgc aaaaatccgg gattagacat atttgttgtt aaagaagcgg aaaaccgtaa
     6541 tggtacagtt tattcgtatg gtggtgttac taagaaaaat caaggcgcct attatgatta
     6601 tataaacgca ccaagattcc aaatcaagag agatgaaggt gacggtattg ctacgtacgg
     6661 tagagtacac tacatttata aagaagaagt atcacttaaa gaattggatt ttaaattgag
     6721 acagtattta attcaaaatt ttgatctgta taaaaagttt cctaaagaca gcaagataaa
     6781 agtgataatg aaagatggcg gctattatac gtttgaactc aataaaaagt tacaaacaaa
     6841 ccgcatgagt gacgtcattg acggtagaaa tattgagaaa atagaagcca atattagata
     6901 attcaataaa acatggataa tagtaaaata tggatagtat agaggagtta ggcagcatag
     6961 gttgcttagc ttcttttttg tgtcgttatg atcggaaatg aagcgggttg atgaaagata
     7021 ataacgacaa taaaacttgt aaaaatagtt gatacctata gtcgcacggt gtcgcattag
     7081 tgacgtgaaa caatgtggaa aacataatta aattgaagga aagtgttaat ggtgaaaaaa
     7141 attagcatag tgttataaaa aatgattaat actgttagga atccattaat tagcttaacg
     7201 ttggttcatg aatagttaaa aagtggttaa ttcatagcgc aatatccggc ttatataatg
     7261 atagtagatt gttcgtatta cgtaattgaa ttaatcatat aagcatatat taagttaaat
     7321 tttaaaaata gattgggaga ataatactat gaaattaaaa acgttagcta aagcaacatt
     7381 agcattaggt ttattaacta ctggtgtcat tacatcagaa ggtcaagcag taaaagcggc
     7441 agaaaagcaa gagagagtac aacatttaca tgatattaga gatttacatc gatactactc
     7501 agcaccaagt tttgaatata gtaatattag cggtaaggtt gaaaactaca atggttctaa
     7561 cgttgtacgc tttaaccaaa aagatcaaaa tcaccaatta ttcttattag gaaaagataa
     7621 agaagaatat aaagaaggtc ttcaaggtca aaatgtcttt gtagtacaag aattaattga
     7681 tccaaacggc agactatcta ctgttggtgg tgtaacgaag aacaataaca aaacttctga
     7741 aactaaaaca caattagttg ttaataaagt tgatggcgga gatttacatg catcaattga
     7801 ctcattttta atccaaaaag aagaaatctc attaaaagaa cttgatttca aaattagaca
     7861 acaattagtt aataattacg gattatataa aggtacatct aaatacggta aaatcattat
     7921 caatttgaaa gacgaaaata aagtagaaat tgatttaagt gataaattgc aattcgagcg
     7981 catgggcgat gtgttgaata gtaaagacat tagaggtata gcagtaacta ttaaccaaat
     8041 ttaaagtaag caatcaatga ctttaaagta ataaattttg aagcagctta acgatgaaac
     8101 gttgaataaa tacgcatatc ctacaagagg agcgtgttta aagtaacgcc gtcgttcggc
     8161 tgttttttat atttaataac gcaggtcacg agtatactaa aaaaaccaaa ttagacgtga
     8221 aagctatgtg ctttgaatat tacttagatg ttcattaaca atgattcaaa gttatttaaa
     8281 cgcacgttaa tgtcagtttg tttcgatgca ctttataata aaggcagatg tttcaaatta
     8341 cgtaatcata acaatccaat acattaagat tggagtaaat aaatatgaaa ttgacagcat
     8401 tagcaaaagc aacattagca ttaggaatat taactacagg tgtgtttaca gcagaaagta
     8461 aagccgttca cgcgaaagta gaacttgatg agacacaacg caaatactat atctatatgt
     8521 taaaagatta ctattctgaa gaaagctatg aaccaacaaa cattagtgtt aaaagtgaag
     8581 attattatgg ttctaacgtt ttaaacttta accatcgaaa taaaaacttc aaagtatttc
     8641 tacttggtga tgataaaaat aaatataaag aaaaaacgca tggtcttgat gtctttgcag
     8701 tacctgaatt aatagatact aaaggtggca tatatagcgt tggcggtata acaaagaaaa
     8761 atgtgagacc cgtgtttggc tgtgtaagtc atccaggttt actagttaaa aaaatcgatc
     8821 ctaaacatgg cttttcggta aatgagttgt tctttattca aaaggaagaa gtatcgttga
     8881 aagaacttga ttttaaaata agaaaaatgt tagtcgaaaa atatagattg tataaaggcg
     8941 cgtcagataa aggtagaatc gttattaata tgaaagacga aaagaaatat gtaattgatt
     9001 taagtgaaaa attaagtttt gatcgtatgt ttgatgtaat ggatagtaag caaattaaaa
     9061 atattgaagt gaatttgaat taattaagca taatagcata atagattaag aagcgactta
     9121 acaacaaaat gtgaattgac atgcatgtcc ttaaataagg aactgtgtta aatacattaa
     9181 tgttgttaag ttgttttttg catttcaaag tgcagaacag agtatcaaca tttgttgtag
     9241 taaacaatga tccagaaaaa caaccacatg taaagagtga aagttaatta atctggccat
     9301 taaaatatat ttgtttttca ttaataataa ttcacgtata tttaaattga ggttaattat
     9361 cgcattaaac gatggacgtt ataattagag tgtatgattc aaattacgta ataaaacaat
     9421 ccaatacatt aagattggag taaataaata tgaaattgac agcattagca aaagcaacat
     9481 tagcattagg gattttaaca acaggaactt taacaacaga ggcttattca ggtcatgcta
     9541 aacaaaatca aaagtcagta aacaaacatg ataaagaagc attacaccga tactacagtg
     9601 gaaactttaa ggaaatgaaa aacattaatg ctttgagaca tggtaaaaat aacttacgtt
     9661 ttaaatatag aggtatgaag actcaagtat tattgcctgg agatgagtac cgtaaatatc
     9721 aacagcgaag acatacaggc ttagatgtgt tttttgttca agaaaaaaga gacaagcacg
     9781 acatatcata tactgttggt ggcgtaacaa agtcgaataa aacacctggg tttgttagtg
     9841 caccattatt aaatgttaca aaagaaaatg gtgaagatgc ttttgtgaaa ggttaccctt
     9901 atgatattaa aaaagaagaa atatcactaa aagaattaga ttttaagtta agaaaacatc
     9961 taattgaaaa atatggtctt tataaaacaa aatcaaaaga tggcaagatt aaaattaact
    10021 tgaaagatgg cagcttttat aaccttgatt taagatctaa attaaaattc aaacatatgg
    10081 gggaagtcat agatagtaaa caaattaaag atattgaagt gaatttgaag taaataatta
    10141 cgaataagaa aaattaattg aagtggctta acgatgaaat gtaaattggt gtgcacacct
    10201 taccaaaggg atgcattaat cgatatcgtc gttaagtcgt ttttttcgtt ttatggattc
    10261 taccccaatt tccataaaga taaaatttcc accaccaaca tcaaaattct caacatcgta
    10321 acatcaccaa atgttataat aaatctatta cacaaagaga taaattactt attcaaaggc
    10381 ggaggaatca catgtctatt actgaaaaac aacgtcagca acaagctgaa ttacataaaa
    10441 aattatggtc gattgcgaat gatttaagag ggaatatgga tgcgagtgaa ttccgtaatt
    10501 acattttagg cttgattttc tatcgcttct tatctgaaaa agcagaacaa gaatatgcag
    10561 atgccttgtc aggtgaagac atcacgtatc aagaagcatg ggcagacgaa gaataccgtg
    10621 aagacttaaa agcagaatta attgaccaag tcggttactt cattgagcca caagatttat
    10681 tcagtgcgat gattcgtgaa attgaaacgc aagatttcga tatcgaacat ttggcgacgg
    10741 caattcgtaa agttgaaaca tcaacactag gtgaagaaag tgaaaatgac tttatcggac
    10801 tgttcagcga tatggatttg agttcaacgc gtttaggtaa caatgtcaaa gaacgtactg
    10861 cgttaatctc taaagtcatg gttaaccttg acgatttacc attcgttcac agtgacatgg
    10921 aaattgatat gttaggtgat gcatacgaat ttcttatcgg gcgctttgcg gcgacagcgg
    10981 gtaaaaaagc aggcgagttc tatacaccac aacaagtatc taagatactg gcgaagattg
    11041 tcacagacgg taaagataaa ttacgtcacg tgtatgaccc aacatgtggt tcaggttcac
    11101 tgttgttacg tgttggtaaa gaaacacaag tgtatcgtta tttcggtcaa gaacgtaaca
    11161 ataccacata caacttagcg cgtatgaata tgttattaca tgatgtgcgt tatgaaaatt
    11221 tcgatatccg taatgatgat acgttggaaa acccagcctt tttaggccat acatttgatg
    11281 cggttattgc gaatccaccg tatagtgcga aatggacagc agattcaaaa tttgaaaatg
    11341 acgaacgatt tagtggttac ggcaagcttg cgccaaaatc caaagcagac tttgccttta
    11401 ttcaacacat ggtacattac ctagacgatg aaggtaccat ggcagttgtg ctaccacatg
    11461 gcgtcttatt ccgtggggct gcagaaggtg tgattcgtcg ttatttaata gaagaaaaga
    11521 actacctaga agccgtgatt ggcttaccag ccaatatttt ctatgggaca agtattccaa
    11581 catgtatttt agtatttaaa aaatgtcgcc aacaagacga caacgtatta tttatcgatg
    11641 catccaatga ttttgaaaaa ggaaaaaatc aaaaccattt aagcgatgcc caagtcgatc
    11701 gtattatagc cacatataag cgtaaagaaa cgattgataa atatagctac agcgcgacat
    11761 tacaagagat tgccgataac gattacaacc taaatatacc gagatatgtc gacacattcg
    11821 aagaagaaga gccaattgat ttagatcaag tccaacaaga tttgaaaaat attgacaaag
    11881 aaatcgcaga aatcgaacaa gaaatcaatg catacctgaa agaacttggg gtgttgaaag
    11941 atgagtaata cacaaaagaa aaatgtgcca gagttgagat tcccagggtt tgaaggcgaa
    12001 tgggaagaga agaagttagg ggatcttaca gatagagtaa ttaggaaaaa taaaaactta
    12061 gaatcgaaaa agcctttaac aatatccgga cagttaggtt taattgatca aacagaatac
    12121 tttagtaaat cagtttcgtc gaaaaatcta gaaaattata cactaataaa gaatggagaa
    12181 ttcgcgtata acaaaagtta ttctaatgga tacccattag gggctattaa aagattaact
    12241 agatatgata gtggtgtatt gtcctctttg tatatttgtt tttctattaa aagtgaaatg
    12301 tctaaagact tcatggaagc atattttgat tcgacacact ggtatagaga agtttctgga
    12361 attgcagttg agggtgcaag aaatcacgga ttattaaatg tttctgtgaa tgattttttt
    12421 actattctaa ttaaatatcc aagtttagaa gaacagcaaa aaataggcaa gttcttcagc
    12481 aaactcgacc gacaaattga attagaagaa caaaagcttg aattacttca acaacagaaa
    12541 aaaggctata tgcagaaaat cttctcacag gaattgcgat ttaagaatga gaatggtaat
    12601 gattatcctg attgggaaag aattaaattt tttgatgtaa ttgataaagt aatagatttt
    12661 agagggagaa caccaaaaaa attaaatatg gaatggtctg acgaagggta tttagcatta
    12721 tcagcagtca atgtaaaaaa aggctatatt gattttaatg tagaggcgaa atatggaaat
    12781 ctagatttat atactagatg gatgagagga aatgaattat ataaggggca agtattattt
    12841 acaactgaag cgccaatggg caatgtagca caggttccgg ataataaagg atatatatta
    12901 agtcaaagaa ctatcgcgtt taattcaaat gaaaaaatca ctgataactt tttagcatca
    12961 ttgttgagct ctgaaaatgt ttataatgat ttattaaaat tgtgtagtgg tgctacagca
    13021 aaaggtgtga gtcaaaaaaa tttaaatcga ctatacgtta ctattccaca ttccatatca
    13081 gagcaagaag agattgctga attctttaga aaaattaatc aattggttga gttgcaaaaa
    13141 tataaaattg aacatactaa aagtcaaaaa caagtgtttt tacaaaagat gtttatttaa
    13201 gcatttgagc acatctatca attaagtatt aataaaataa tcttaaattg gccgattttc
    13261 cttatgtaac ttttagtaac taaaaatacc cgattcgcta tgataaaaaa gcgaacatta
    13321 aatttagaca ctgtgaaagc gcagtgtctt ttttgtgtcg aaattgtgta cagaataagt
    13381 ggttaaataa agattaagtt gagataaagt gttattcgta aataaaagag agtagatcga
    13441 taggaattga atgatattag ttaactattt attaaattac ttaataatga ttaattttta
    13501 gttaaagtaa gtttaatgtg aagcacgacc attgctcatt ataatgaatg aggattgttc
    13561 gtattgcgta atagaataaa tcaaatagac taaaaattgg gagcatagaa ttatgaaatt
    13621 aaaaaatatt gctaaagcaa gtttagcatt agggatttta acaacaggga tgattacaac
    13681 tactgctcag ccagtaaaag caagtacatt agaggttaga tcacaagcga ctcaagattt
    13741 aaaagattat tataaaggac aaagttttga tctgaaaaat ataggaggat ataaggaagg
    13801 caacaaggtt actttcatat atcacaatca acaaattgat gtgacactaa caggtaatga
    13861 aaagttagaa tttaatgacg atggagaaat atctaatgtt gatgtatttg tggtcagaga
    13921 aaattctgat agatcaggta tcacaagttc taatggagga ataacaactt caaataaaca
    13981 agatatcata gataatgtca aaagtgtgaa catgcaaata actaaaaaaa ctagtagtaa
    14041 tactgaaact gtaacttcat cttattacag tataagtaaa gagaatatct cattaaaaga
    14101 acttgatttc aaattaagaa aacacttaat cgacaaaaac ggtctttata aaacagaacc
    14161 taaaaacagc aaaattagag ttaccatgga agatggtggg ttttacacat ttgaattgaa
    14221 taaaaagtta caaacaaacc gcatgggtga tgtaattgat ggtactaaaa taaaagaaat
    14281 taatgttgaa ttaaactaat aatattcgag ggagtaaatc atgagggaaa attttaaatt
    14341 acgtaaaatg aaagtcggtt tagtatcagt tgcaattact atgttatata tcatgacaaa
    14401 cggacaagca gaagcatcag aaactaatca aaagccaagt acaaatcaag aatcaacagc
    14461 tgttacacat actgaacaaa attcaaaaga aacaaaaacg gcagagtctt ctaagaactt
    14521 tgttaattta gaccctatca aaccaggaga tcaaaaggta acagggacta ctatgccgaa
    14581 tcatattata ttacttaaca tagatggaaa aagtgttgat tcagtagatg gagaggacag
    14641 ttcatttata acttcaaaag aagatgggaa gtttgagtat cctctaaata atcgtaaaat
    14701 agttcacaac caagaaattg aagtatcatc ttcatcttta gatgatttag gtgaagatga
    14761 tgaagaggta gaagaagatt ccactgaaaa atctgaagct gaagaagaaa gtacaggtgc
    14821 taaagttact tacacaacac cgcgatatga aaaagcgtat gaagtaccgg aaaaacgatt
    14881 agaagaaaaa gatggacatc accaagtttt catcgaacct attactgaag gatcaggtat
    14941 tattaaaggg catacgtctg taaaaggtaa agttgcttta tctattaata ataaatttat
    15001 taattttgaa acaaattcta atggtggccc aaataaagaa gaagcgaaat ctggatcaga
    15061 aggaatctgg atgcctattg atgataaagg atactttaat tttgacttca aaacgaaacg
    15121 tttcgataat ttagagttaa aagaaggtaa tgacatttca ctaacatttg cacctgatga
    15181 tgatgaagat gcattaaaac ctttaatttt caaaactaaa gtaacaagtt tggaagatat
    15241 tgataaagca gaaactaaat atgaccatac taaagtcaac aaagtgaaaa ttttagataa
    15301 tgttaaagaa gatttacatg ttgatgaaat atatggaagc ttatatcata cagacaaagg
    15361 taaaggtatt cttgataaag aaggtactaa agtaattaaa ggaaagacta aattcgcgaa
    15421 tgcagtagtg aaggtagact ctgaactagg tgaagcacaa ttattccctg atttacaagt
    15481 aaatgaaaaa ggtgaattta gctttgactc acatggtgct ggttttagat tacaaaatgg
    15541 agaaaaacta aactttacag tagttgatcc tattacaggt gagctgttga gtaatgagtt
    15601 tgtttctaaa gagattgata ttgaagaaac accagaagaa aaagcagacc gtgaatttga
    15661 tgaaagactt gaaaatacgc ctgcttacta caagttatac ggcgataaaa tagttggttt
    15721 cgatactaac gatttcccga ttacttggtt ttatccatta ggtgaaaaga aagttgaacg
    15781 tataacacct aaattagaaa aataattaat aaaataaaac agcttaatga tgtaatgaaa
    15841 ttagtgagtt aatcactgac ttcaacgtca ttgagctgtt ttttgtgctt tgttacaaag
    15901 cattattgaa tttattttac gtgttcatat tttgaaacat caaagccgtc ttgtttagct
    15961 ttgttgataa tgtctttgat tgaatgtagt cctttatcgg cgaagtatga tcttaagttg
    16021 tcttttgtag cttggtcagc attcttatct aataacacat caatataact taattcatgt
    16081 tctaagaagt ttgcatcata atgtagtacg agtccatttt gagaataaac tttcgcatct
    16141 gcttgattac catatccaac aacgccagtt gctaaaacac ctaccattgc cgtagctact
    16201 aaaacctttt taaatttcat atccatcact cctctaaaaa ttgtaactcc atcataacac
    16261 tgaatattaa gaaaattacg tttattaagt cgatttaatg atttttaata aatagttaaa
    16321 gtgacaaata ttgctaaaaa gcaattaatc tctaatacga tacttatgaa ttttcctact
    16381 aaaaccttga tttcaaagag ggtttaaatc aaatgaaaca ataataaaaa aatgatgcaa
    16441 cttaataata agtacaaatt taattaagaa attaaattga ttgtatatgt atattttggt
    16501 aacgtaaaag agaaatatac aaaataatta attatttata tgaaaagaga atataaatga
    16561 agtataaaac agagagacgt gaagcgatgg gatatttaaa aaggtttaca ttgtacataa
    16621 gtatttttat tttaatagtt gttatagcag gttgtggtaa aagtgatgaa acaaaagaag
    16681 attccaaaga agaacaaatt aaaaagagct ttgcgaaaac gttagatatg tatccaatca
    16741 aaaatctcaa ggatttatac gacaaagaag gctatagaga tggcgaattt aaaaagggcg
    16801 ataagggtac atggacttta ctcacaagtt tttcaaaaag taacaaacca ggcgagatag
    16861 atgatgaagg tatggtgcta tatctaaata ggaataccaa aaaggcaaca ggttattatt
    16921 ttgtaaataa aatttatgat gatattagca aaaatcagaa tgagaaaaaa taccgtgttg
    16981 aacttaaaaa taataagatt gttcttttag ataatgtaga agacgaaaaa cttaaacaaa
    17041 aaattgaaaa ttttaaattt tttagtcagt atgccgattt taaagattta aaaaattatc
    17101 aagatggaag tataacaact aatgaaaatg taccgagcta tgaagcagaa tacaaattga
    17161 ataatagtga tgaaaatgta aaaaaactta gagatattta tccaattaca acgaaaaagg
    17221 ctccaatatt aaagttacat atagatggtg atataaaagg aagttcagtt ggatacaaaa
    17281 aaatagaata taaattttca aaagttaaag atcaagagac aacattaaga gattatttaa
    17341 attttgggcc gtctgatgaa gatatctaaa gtttattaga tatagaaata attctaaaac
    17401 agagagacgt gaaatgatgg gatatttaaa aaggattgga ttgtgcataa gcctgttgat
    17461 tttaagcatt tttgtaacat cttgtgatag tgataataag atcactggag attcaaaaga
    17521 agcacaaatt aaaaagagtt ttgcaaaaac tttagatatg tatccaatta agaatctcga
    17581 ggatttatac gacaaagaag gatatagaga tggtgaattt aaaaagggag acaaagggac
    17641 gtgggttgtt agatctgaaa tgataattca accaaaaggt aaaagtttaa cttcaagagg
    17701 catgatatta tacatgaata gaaatactag gaccacaact ggttattttt caatagaaga
    17761 aatagattca agaaaaaacc ttgacgagag agaaacagaa aaaaagtatc ctgtgaaaat
    17821 tataaacaat aaaattattc caaccgtaaa gataaaagat gaaaagttga aaaaggaaat
    17881 tgaaaacttt aagttttttg ttcaatatgg gagttttaaa ggaatagaga attatgaaaa
    17941 tggtcacatt tcctacaatt ctgaaggtcc tatttattcc gcgagatatc aactgaaaaa
    18001 tgatgattat aatgttaaag aattacgaaa aagatatgat ataccaacag aaaaggcgcc
    18061 caaattattg ttgaaaggtt cgggggattt gaaagggtct tcagttggat ataaggaaat
    18121 tgaatttata tttctagaaa ataaaaaaga aaatatatat ttttcagatg gattaaactt
    18181 aattccaggt gactaaaaat aacacaaagg ttgatggtgc tcaacaaata ttaacatgat
    18241 agagagaagt gaaaaaatga gggattcaaa aagagtagta ttgtacataa gcattatggt
    18301 gttgagtatt tttataattg gttgtaatag ttcaagcgag aatgcaaaaa aatcaaaaga
    18361 agactcaaaa gaagaccaaa tcaaaaagag ttttgcgaaa acgttagata tgtatccaat
    18421 taagaatctc gaggatctat atgacaaaga aggatatcga gatggcgaat ttgaaaaagg
    18481 tgataagggg acgtggacga tatatacaga ttttgccaaa agtaataaag cgggtgaatt
    18541 ggatagtgaa ggtatggtat tacatttgaa tagaaatacg cggacggcta aaggatatta
    18601 ttttgtaaga aaaacttata acgaagagga taagatgctg caaaggaaag attatcgtat
    18661 taaacttaaa aataataaaa ttgtcctttt agataaagta gaagataaaa aactcaaaca
    18721 taaaatagaa aattttaaat tttttgggca atatgcagat tttaaagact tgaaaaatta
    18781 taaaaacggg aaaatatcga gcaatgaaaa tgttccatat tatgaagcag agtacaaaat
    18841 agaaaatagt gatagaaatg taaaaaaact tagaaatgtt tatccaatta caaccagtga
    18901 atcgccaact ttaaaatttc atatagatgg ggatataaaa ggtagctcga taggatataa
    18961 aaaagtggaa tatatctttt caaagcaaaa agataaccaa atttctgtca gtgatttttt
    19021 aaattttaac ccatcaaaag ataatcagta gagattcaat aaaaaatcag acaagaaaga
    19081 tgtggaataa tgagaaattt aaagagagac gtattgtaca taagcattat ggctttgagt
    19141 atttttataa taggctgtga taaatctagt gttactgctg aaaaagcaaa agaagactca
    19201 aaagaagacc aaatcaaaaa gagctttgcg aaaacgttag atatgtaccc tattaagaat
    19261 ctcgaggatt tatacgataa agaaggatac cgtgatggcg aatttaaaaa gggtgataag
    19321 gggacgtggg ttattagatc tgaaatgaca acagaactaa aagatgaaaa tatggaatcc
    19381 aaaggtatgg tcgtacgttt aaatagaaat accagaacaa ctacaggtga gtattttgtt
    19441 aggataatca aagaagacaa tgagggcaag gtatatagtg atgaacgaaa atacccagtc
    19501 aaaattgata ataaaaaaat tattccatta aaattaatcg atgatgaaaa agttaaaaaa
    19561 gaaattgaag aatttaaatt ctttgtacaa tatgggaatt ttaaagaatt ggaaaactat
    19621 aaagatggta aagtgtcata taatccagaa gtaccaattt actctgcgca atatcaattg
    19681 aaaaacagtg attataatgt agagcaacta cgtaagcgat ataatatccc aacaagaaag
    19741 gcgcctaaat tgttattgaa aggctcaggt aatttaaaag gatcctcagt cggatataaa
    19801 aatattgaat ttacctttgt tgaaaataag gaagaaaata tttacttcac agatagtatt
    19861 aatttcaacc caagtgagga taaataagta tatttagtat tataaaaaac acgttagaac
    19921 atttaataat aaagttaaat gaattacatg tattaaattg aataagttag ttttgtttga
    19981 taacataaaa gtgcaata
//
LOCUS       DAHHOJ010000046        19787 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.046, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000046 DAHHOJ010000000
VERSION     DAHHOJ010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19787)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19787)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19787
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.046"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..1516
                     /locus_tag="NBH32_001396"
     CDS             <1..1516
                     /locus_tag="NBH32_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515001.1"
                     /translation="KLKIIIPIIIVLLLIGGIAWGVYAFFANTPKNTYLKSEQQTAKM
                     YKDYFNDRFENEVKFQEKMKDNSFLSSLELSADASDEIVKGLGIPKSVVNASKIKMSY
                     GHDPKKEKSMINLEPTIADSELGKFQLAADKDKHYFESPLFKGKYSVNNSDLLSTYSK
                     LTGEDEETAKENGITNQQLNLNTLFSNAQAQQSDYSKIAEKYSELIVDKLDDDNFDKG
                     KKEEIKVNGEKYKVRPVTLTLSRADTKKITLAVLEEAKKDKDLKKLMEEQGATKDFEK
                     DIKKAIDDVKETKKDEFAKIQSKIYTEKHTIVKREITITDKENNKTKIKGTNTLEDNK
                     LKLDYALDFDQDKYTYAEAKYTIKGVSSKEKDNKYSDKYEFGKKTEYDESKIKLDNQE
                     KVDGTKRQDKGKITVALDKYSDENEFTFENNIDSDVKNNTQKSTLNIGIKYAEEPINF
                     ILKSSTKLKADIDFDDSGAKDFNSLSSKDREKLEKEIEKNGGKMFESILKKASK"
     gene            1584..2750
                     /locus_tag="NBH32_001397"
     CDS             1584..2750
                     /locus_tag="NBH32_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515002.1"
                     /translation="MKTIHLFRIYHSFLLKKWYLIIYLLFILAALVITLTTIQHVTED
                     DNHFNIGVVDKDQSSETKLILNSIGKGSNLGKNVSIKAYDDKQAHTLLKKHKLQGYFV
                     FDKGMTKAFYKQGELPISVYTYDQQSMKSVVLSQLTDSVYQRLMRSMGGILAFQDLAP
                     KASHSDSINVMTDLLITGLNRSGAFNLEPIHLYDTGSYYAITGFLTTVFIFALSLFTV
                     LKMNQDTVLKARLKMFHFSKERLLIIRALITWFYTMLWSIVGVVWIVFSIPNTFELYN
                     WPTLAIHLSYYVTFLILWLLLIELLTTGLLNSISKVILAIVILVLSGLTIPTIFLQHI
                     ANGVFNIQPFAVVTNQLLEIILNNYILELHPSFYLSFIALLIINLAVLVWRYRQ"
     gene            2747..3667
                     /locus_tag="NBH32_001398"
     CDS             2747..3667
                     /locus_tag="NBH32_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515003.1"
                     /translation="MKPYIQLVVFKQWLQYILLVTTIVIALVLIGIGYRVAHDNFKIP
                     ITIQDLDQTTASKSFVNKIKQSDYVTIKKVDEDESYIEDDVTKKEAILSMQIPKGFSQ
                     KLKENRLKETIQLYGRDDFIGGIAVEIVSSSLYEQQIPNIIYEHLEDMKQHQSIDAIN
                     KSYHKHTPESKIKFVSLTKQAQHSISISLIFAVILFVSAVQVVLHYRLNQQAALQRLS
                     QYHLSRFKLYSTYVMTHTILLLLVLLAVSLYLSQPLSLIFYLKSLLLILIYEIGIVFI
                     LFHIQTISHRLFMTFIYALAMGIVYLTIFM"
     gene            3681..4340
                     /locus_tag="NBH32_001399"
     CDS             3681..4340
                     /locus_tag="NBH32_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515004.1"
                     /translation="MIEINNLSKRYRNKQIFNHLTMSFDSNRLTVLLGDNGAGKSTLL
                     RMIAGIEKANDGTINYFGEKWNQRQIQNHIGYVPQDIALFEHMTVAENIKFFKSLCKN
                     PINDTTINEYLQQLNFDDTSAKVSTLSGGNKRKINILVGLLGKPRILILDEPTVGIDL
                     KSRHDIHQLLNIMKSKCLIILTTHHLDEVEALADDIKLIGQDPFYQHVLEDKQWTYTY
                     Y"
     gene            complement(4392..5339)
                     /gene="lytM"
                     /locus_tag="NBH32_001400"
     CDS             complement(4392..5339)
                     /gene="lytM"
                     /locus_tag="NBH32_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine-glycine endopeptidase LytM"
                     /protein_id="HCD1515005.1"
                     /translation="MKKLTAAAIATMGFATFTMAHQADAAETTNTQQAHTQMSAQSQD
                     VSYGTYYTIDSNGDYHHTPDGNWNQAMFDNKEYSYTFVDAQGHTHYFYNCYPKNANAN
                     GSGQTYVNPATTGDNNDYTASQSQQHINQYGYQSNVGPDASYYSHSNNQAYNSHDGNG
                     KVNYPNGTSNQNGGSASKATASGHAKDASWLTSRKQLQPYGQYHGGGAHYGVDYAMPE
                     NSPVYSLTDGTVVQAGWSNYGGGNQVTIKEANSNNYQWYMHNNRLTVSAGDKVKAGDQ
                     IAYSGSTGNSTAPHVHFQRMSGGIGNQYAVDPMSYLQSR"
     gene            complement(5665..6657)
                     /locus_tag="NBH32_001401"
     CDS             complement(5665..6657)
                     /locus_tag="NBH32_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD1515006.1"
                     /translation="MCTGFTIQTLNNQVLLGRTMDYDYPLDGSPAVTPRNYRWTSRTG
                     TTGQTQYGFIGTGTDMEGFIYGDGVNEHGVAISTQYFRGYSSYGSTHKADAMNITQNE
                     IVTWILGYTTSIEDMKQQASQIHVVAVYLNDIGEVPPLHYHVSDATGQSVEVSFKEGE
                     VVIKDNPIGVLTNHPDLDWHYSNLRQYINISPYPATANLLEGVTIEPLGNEAGTFGLP
                     GGFTSTERFVRMAFMKANIAQNNDKEMDLMNAFYLLDAVNIPIGIVRPHDADNHYTMY
                     QTVINLTTRTLYIKYYGSNEIVALKLTDDLINRKDMTIFKPEKHITIRKLNDNQ"
     gene            6891..8267
                     /locus_tag="NBH32_001402"
     CDS             6891..8267
                     /locus_tag="NBH32_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515007.1"
                     /translation="MNNTQSSPRSNIIIAIMLSALTYWLFAQSFINIGPLVGQTYQTS
                     PAVLNLSISLTSFATGIFMVAAGDIADKIGQLRMTYMGLIISMFASLLLIISDITALL
                     IIGRILQGLSAAILLPSTVGVLNNQFKGEHLRRAISYLMISTVGGIGLAGVIGGLIAT
                     NFGWQTNFIISIVIAFIAILLLKGTPEKVSQHSHRHPFDYKGMSIFAVMIGSFTLLLT
                     QGFEQGWFSTFSFICLSIFIITTLIFIIIERRHEVPFIDFSVLRNRPFIGAFLNNFVL
                     NSGLGVTVVFFIYAQTHLGLSAAQSGLVTLPYAIVAVAMIRLGEKATLRFGGKLMLII
                     GPLFPVIGITIISMTQLSASQYVIAVIIGFVICAIGNGLVATPGLTIAIFSMPNEKVG
                     LATGLYKMSGTLGGAFGIALSTTVFSMLQLNYAPSVAATVTFIVSIVLMILGSLSAYM
                     IIPKTVKS"
     gene            complement(8395..8787)
                     /locus_tag="NBH32_001403"
     CDS             complement(8395..8787)
                     /locus_tag="NBH32_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515008.1"
                     /translation="MQFKLKEEEIISFLELKYPEKEFEYGRLLVGQHKRDDLDVYYFG
                     DTFLMCTIISFKTFEIKETVELSYDAVNRIVLKDGWLFRKMRIETMQKVLKYGTSKLM
                     LTDFQKENYNKYIQGQKQRVIFENGHFV"
     gene            complement(8837..9017)
                     /locus_tag="NBH32_001404"
                     /pseudo
     CDS             complement(8837..9017)
                     /locus_tag="NBH32_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498838.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
     gene            9168..10166
                     /locus_tag="NBH32_001405"
     CDS             9168..10166
                     /locus_tag="NBH32_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1515009.1"
                     /translation="MKKVSIKDVAREAGVSVTTVSHILNHNDSRFSATTIKNVHAVSE
                     RLGYAPNKHAKQLRGSKIQTIGVILPSLTNPFFSALMQSIHDHKPSDVDLCFLTSTAT
                     DLYDNIKHLIDRGIDGLIIAQYISSPDALNNYLKKHHVPYVVLDQNDHQGYTDFVRTN
                     EYQGGQLAAQHLIELGHNNMIVVAPYDMMANMSTRVAGFVDTLRANQLPEPQIVHTEL
                     SKRGGLTIVDDIMVQSATAIFAINDELAIGILRGLIEHGISIPKDISLIGYDDIDYAA
                     YVSPPLTTVAQPITDIGKTSLTLLLQRLQHLDKSIDMIELPTTLKIRATTGYHLSN"
     gene            10398..11279
                     /gene="rbsU"
                     /locus_tag="NBH32_001406"
     CDS             10398..11279
                     /gene="rbsU"
                     /locus_tag="NBH32_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose transporter RbsU"
                     /protein_id="HCD1515010.1"
                     /translation="MSIVALLIGLGPLIGWGFFPTVASKFGGKPVHQIIGATVGTLIF
                     AIILAVVTSSGFPTGTNLLFALLSGAGWGFGQIITFKAFELVGSSRAMPVTTAFQLLG
                     ASLWGVFALGNWPGIGHKIIGFTALVVILIGARMTVWSERKEASNAKNLRRAVVLLLI
                     GEFGYWLYSAAPQATSIDGLTAFLPQAMGMVIVAVIYGFMNMKAENPFRNKITWLQII
                     SGFFFAFGALTYLISAQPNMNGLATGFILSQTSVVLATLTGIYFLKQHKTSKEMVITI
                     IGLVLILVAASVTVFIK"
     gene            11294..11698
                     /gene="rbsD"
                     /locus_tag="NBH32_001407"
     CDS             11294..11698
                     /gene="rbsD"
                     /locus_tag="NBH32_001407"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCD1515011.1"
                     /translation="MKKTAVLNEHISKAIATIGHFDLLTINDAGMPIPNDHRRIDLAV
                     TKNLPRFIDVLATVLEEMEIQKIYLAEEIKEHNPTQLQQIKQLISSEIEIIFIPHEEM
                     KSNLAHPLNKGNIRTGETTPYSNIALESNVTF"
     gene            11726..12640
                     /gene="rbsK"
                     /locus_tag="NBH32_001408"
     CDS             11726..12640
                     /gene="rbsK"
                     /locus_tag="NBH32_001408"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCD1515012.1"
                     /translation="MTNKVVILGSTNVDQFLTVERYAQPGETLHVEEAQKAFGGGKGA
                     NQAIATARMQADTTFITKIGTDGVADFILEDFKAAHIDTSYIIKTAEAKTGQAFITVN
                     AEGQNTIYVYGGANMTMTPEDVINAKDAIINADFVVAQLEVPIPAIISAFEIAKSHGV
                     TTVLNPAPAKALPNELLSLIDIIVPNETEAELLSGIKVTNEQSMKDNANYFLSLGIKT
                     VLITLGKQGTYFATKNQSQHIEAYKVNAIDTTAAGDTFIGAFVSRLNKSQDNLADAID
                     FGNKASSLTVQKHGAQASIPLLEEVNQV"
     gene            complement(12737..12871)
                     /locus_tag="NBH32_001409"
                     /pseudo
     CDS             complement(12737..12871)
                     /locus_tag="NBH32_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498833.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            12891..13652
                     /locus_tag="NBH32_001410"
     CDS             12891..13652
                     /locus_tag="NBH32_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1515013.1"
                     /translation="MSKEAGHTFLAKLGKTRLRPGGKEATDWLIQQGAFSQDKQVLEV
                     ACNMCTTSIYLAHTYGCHIQGVDINKKALEKAQENISAAGLESYIQVQQANAVKLPFD
                     DNQFDIVLNEAMLTMLPIAIKEKALREYYRVLKPGGILLTHDIVIVNESHATHVVKSL
                     SAAINVNVSPQTKLGWLDLYHQAGFNHVHYHTGPMSLMTPKGLIYDEGIVGTLKIINN
                     ALKKENRPMFCKMFKTMTKLRKDMNYITFVAKKEH"
     gene            complement(14147..15583)
                     /locus_tag="NBH32_001411"
     CDS             complement(14147..15583)
                     /locus_tag="NBH32_001411"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCD1515014.1"
                     /translation="MTKLPQNFMWGGALAANQFEGGYDKGGKGLSVIDVMTSGAHGKA
                     RQITESIDPNHYYPNHEGIDFYHRYKEDIALFKEMGLKCLRTSIAWTRIFPNGDEDVP
                     NEEGLAFYDRIFDELIAQGIEPVVTLSHFEMPLHLAKHYGGFRNREVVDYFVHFARVV
                     FERYKDKVTYWMTFNEINNQMDTSNPIFLWTNSGVALTENDNPEEVLYQVAHHELLAS
                     ALAVRLGKEINPKFKIGTMISHVPIYPYSCHPKDMMEAQIANRLRFFFPDVQVRGYYP
                     SYAKKMLARKGYDFGWQEGDDSILQQGTVDYIGFSYYMSTAVKHDVDTTVENNIVNGG
                     LNHSVENPHIATSDWGWAIDPDGLRYTLNVLYDRYQLPLFIVENGFGAVDEVVDGHIH
                     DDYRIEYLKVHITAAIEAVDQDGVDLIGYTPWGIIDIVSFTTGEMKKRYGLIYVDRDN
                     DGHGTMERLKKDSFYWYKQVIASNGDKL"
     gene            complement(15599..16390)
                     /locus_tag="NBH32_001412"
     CDS             complement(15599..16390)
                     /locus_tag="NBH32_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1515015.1"
                     /translation="MSNKYKDQAQDILTAVGGVENIVDVTYDTKGITIHMQHTIPSTA
                     NEVKQIVDVTSVAENDAQLVIKLNGNVDEVYQQLQRLIKDANVEEGANTDNINSQDTS
                     YTPQAKVTTPILVKAPIAGRRILLKEVRDSIFREKMVGEGLAIKAHEESKVIAPFNGL
                     ISMIVPTKHAIGIRSEEGVDIVIHIGVNTVDLEGKGFKCFVKQNDRVEAGQTLLQFDQ
                     QYIQEQGYNSDVIVVISNSADLGKVELTMNEIITTEDVIFKIFKN"
     gene            16539..17243
                     /locus_tag="NBH32_001413"
     CDS             16539..17243
                     /locus_tag="NBH32_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1515016.1"
                     /translation="MLKYEHIAKQLNAFIHHSNFKPGDKLPSVTQLKERYQVSKSTII
                     KALGLLEQDGLIYQAQGSGIYVRNIADANRINVFKTNGFSKSLGEHRMTSKVLVFKEI
                     ATPPKSVQDELQLNADDTVYYLERLRFVDDDVLCIEYSYYHKEIVKYLNDDIAKGSIF
                     DYLESNMKLRIGFSDIFFNVDQLNSSEAALLQLSKGEPCLRYHQTFYTMTGKPFDSSD
                     IVFHYRHAQFYIPSKK"
     gene            complement(17469..19760)
                     /locus_tag="NBH32_001414"
     CDS             complement(17469..19760)
                     /locus_tag="NBH32_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000062635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HCD1515017.1"
                     /translation="MSMQSSYKRLVKVLLTILVIVFSILLVGGWYIFKNEAPRPTKIV
                     DQQGHTLVTKGELISGQAIYEKYGLTDYGSYLGNGSYLGPDYTAEALHHYLIGMRKYY
                     AQDIYHKQLHKLNHAELAVVKDKVMKEIRINRYSEKEDQLVLTPGQVYGLKYLQDYYK
                     KEFVNNPKQVGLNENMIKQFQNDDYMVAGNKVEHLSQFFFWGAWLSSTDRPGKTFSYT
                     NNWPYDVDAGNTLPSAGILWTAISVTLLIAGLAFIIYIQKRYQFDMKPTYESERELPK
                     IEVDRKITDSQRKVGKYLVVVMLLFLVQILLGELLAHYYVENKFFGIEIQRLFPFNIA
                     KTWHVQLVIFWVATTWLAAGIYIVPKVLGKEPKKQGVLVDILFWALIIVVMGSMIGEW
                     GHILGWIDSHWWLFGHFGWEYIELGKFWQILFIVGMVLWVIILCRGFLPAIRNKVQVN
                     HDKRHLLTLLFVGAIAIPLFYLASLFIMPNSHVTFADYWRWWIVHLWVEGIFEAFAVV
                     LIGFLMVNMKLTTIETTIRALYFQLILLLGTGIVGMGHHYYWQGDHSIWLALGSCFSA
                     LEVVPLCLLIWEAYTHYRLYKNSDIAFPYKGTFIFLASTGLWNAIGAGALGFLINTPA
                     VNYFEHGTQWTAAHAHGSMAGVYGMFSIAIALYVFKNITKKEFWTPKVEKWIKISCWA
                     LNIGLAGMVFVTLMPVGYIQLKDALEHGYWHSRLTSFYEQPLVKAIMWGRMPWDIIFT
                     VGVIILVVIFIRGYRHLKPKYRV"
BASE COUNT         6557 a         3635 c         3086 g         6509 t
ORIGIN      
        1 aaagttaaaa attataattc ctattattat tgtcttatta ttaataggtg gaatcgcatg
       61 gggagtttat gcattttttg caaacacacc gaaaaataca tacttaaaaa gtgaacaaca
      121 aactgcaaaa atgtataaag attattttaa tgaccgtttt gaaaacgaag tgaagttcca
      181 agaaaagatg aaagataatt catttttatc ttcattagaa ttaagcgcag atgcatcaga
      241 tgaaattgtt aaggggcttg gtattcctaa atctgttgtt aatgcttcga aaattaaaat
      301 gtcatatgga catgatccta aaaaagagaa atcaatgatt aatcttgaac caacaatagc
      361 agactctgaa ttagggaaat tccagttagc tgcagataaa gataagcatt atttcgaatc
      421 accattattt aaagggaaat atagtgttaa taattctgat ttattatcaa cttattcaaa
      481 acttacaggt gaagatgaag aaacagcaaa agaaaatggt attacaaacc aacaactaaa
      541 tttaaatact ctttttagta atgctcaagc acaacaaagt gactacagca aaattgccga
      601 aaaatattcc gaacttattg tcgacaaatt agatgacgat aattttgata aaggtaaaaa
      661 agaagaaatt aaggttaatg gtgaaaagta caaagttaga cctgtcacgt taacacttag
      721 cagagctgac actaaaaaaa ttacattagc tgtattagaa gaagctaaaa aggataaaga
      781 ccttaaaaaa ttaatggaag aacaaggtgc tacaaaagac tttgaaaaag acatcaaaaa
      841 agcaattgac gatgtcaaag aaactaaaaa agatgaattt gctaaaattc aatctaaaat
      901 ttataccgaa aaacatacga ttgtaaaacg agaaattact attacagaca aagaaaataa
      961 taaaactaaa atcaaaggta ctaatacttt agaagacaat aagttaaaac tagattacgc
     1021 acttgatttc gatcaagata aatacacgta tgctgaagcg aaatatacaa ttaaaggcgt
     1081 atcttctaag gaaaaagaca ataaatacag tgataaatac gaatttggta aaaagacaga
     1141 atatgatgaa tcaaaaatca aattagataa ccaagagaaa gtagatggca caaaacgtca
     1201 agataaaggt aaaattactg tcgcattaga taaatatagc gacgaaaatg aattcacttt
     1261 tgaaaataat atagattctg acgtaaaaaa taacactcag aaatctacgt taaatatcgg
     1321 catcaaatat gctgaagaac caattaattt cattttaaaa tctagcacaa aattgaaagc
     1381 agatattgat tttgatgata gtggtgcgaa agatttcaat agtctatctt caaaagatcg
     1441 tgaaaaactt gaaaaagaaa tcgaaaaaaa tggcggcaaa atgtttgaat caattttaaa
     1501 aaaggcatct aaataactaa aatataaaaa taagattgga aggtcatctt gcctttcaat
     1561 cttatcattg gaggagtggt taaatgaaaa caatacattt gtttcgcatc taccactctt
     1621 ttttattgaa aaagtggtat ttgattatct atttactatt tatattagcg gcactcgtta
     1681 ttacattaac gacaatccaa catgtaacag aagatgacaa tcattttaat ataggtgtcg
     1741 tagataaaga tcaatcaagt gaaacgaaat taatcttaaa ctctattggt aaagggagta
     1801 acctaggaaa aaatgtgagc attaaagcat atgatgataa gcaagcacat actttgttaa
     1861 aaaaacataa acttcaaggt tattttgttt ttgataaagg tatgaccaag gcattttata
     1921 aacaaggcga actaccaatt tcagtatata catatgatca acaatccatg aaaagtgtcg
     1981 tgctatctca gctaacagat tctgtttacc aacgtcttat gcgatcaatg ggtggcatct
     2041 tagcttttca agacttagca ccgaaagcat cacattctga cagtatcaat gttatgactg
     2101 atttgctgat tacaggatta aaccgttcag gtgcatttaa cttagaaccg attcatttat
     2161 acgatacggg cagttattat gcaattacag gatttttaac aacggtattc atctttgcac
     2221 tatctttatt tacagttttg aaaatgaatc aagatactgt attgaaagcg cgattgaaaa
     2281 tgtttcattt ttctaaagag cgtttgttaa tcattcgtgc gttgattaca tggttttata
     2341 ctatgttatg gagtatcgtt ggtgtagttt ggattgtgtt cagtattccg aatacctttg
     2401 aattatataa ttggccaacg ttagccattc atttaagcta ttatgttacc tttttaatac
     2461 tgtggttatt actcatcgaa cttttaacta caggcttgtt gaatagtatt agtaaagtca
     2521 tattagctat tgtcatcctg gtgttatctg gtttaacaat acctacgatt tttttacaac
     2581 atatagcaaa tggcgttttc aatattcaac cgttcgcagt tgtaacaaat caattgttag
     2641 aaattatttt aaacaactac attttagaac tgcaccctag tttctatctt agcttcatcg
     2701 cacttttgat aattaattta gctgtattgg tttggaggta tcgtcaatga agccttatat
     2761 acaacttgtt gtgttcaagc aatggttaca atacatcttg ctcgtaacaa ccattgtcat
     2821 cgcactcgta cttattggta tcggttaccg tgtagcacat gacaacttca aaataccgat
     2881 taccattcaa gatttagacc aaaccactgc atcaaaatca ttcgtcaata aaattaaaca
     2941 atctgactat gtaactatta aaaaagtcga tgaagatgaa agctatattg aagatgatgt
     3001 tactaaaaag gaagctattt taagtatgca aattcctaaa ggtttctctc aaaaattaaa
     3061 agagaaccgt ttaaaagaaa cgatacagtt atatggtaga gatgacttta taggtggtat
     3121 tgctgtagaa attgttagta gttcattata tgagcagcaa attcctaaca ttatttatga
     3181 acaccttgag gatatgaaac agcatcaatc catcgatgct atcaacaagt cctatcataa
     3241 acatacacct gaatctaaaa tcaaatttgt gtcgcttact aaacaagcac aacactctat
     3301 ttcaattagc ttaatctttg cggtgatttt atttgttagc gctgttcaag tagtacttca
     3361 ttatcgttta aaccaacaag cagcattgca acgattatca caatatcatt taagccgttt
     3421 caaactatat agtacttatg taatgacaca tacgattttg ttattgttgg tactattggc
     3481 agttagtcta tatttgtctc aaccactcag cttaatattt tacttaaaat cactgttact
     3541 tatattgatt tatgagatag gtatcgtctt tatcttattc catattcaaa caataagtca
     3601 tcgattattc atgacattta tatatgcact tgctatgggt atcgtatact tgaccatttt
     3661 catgtaaagg agcgtaactg atgatagaaa ttaataacct ttcaaagcgt taccgtaaca
     3721 aacagatttt caatcattta accatgtcct ttgatagtaa tcgattaacc gtattacttg
     3781 gtgataatgg cgctggaaaa tcaacattac ttcgtatgat tgctggtatt gaaaaagcta
     3841 atgatggaac tatcaactat ttcggcgaaa aatggaatca aagacaaata caaaatcaca
     3901 tcggttatgt gccacaagac attgcgttat ttgaacacat gacagtggct gaaaacatta
     3961 aattttttaa atcactttgt aaaaatccaa ttaacgatac aactatcaac gaatatttac
     4021 agcaattaaa ttttgatgat acgtctgcca aagtatctac attgtccggt gggaataaac
     4081 gtaaaattaa tatattagta ggtttactag gtaaacctcg aattctcatt ttagatgaac
     4141 cgacagttgg tattgattta aaatctagac atgacatcca ccaactactt aacattatga
     4201 aatctaaatg tttaattata ttaactaccc atcatttaga tgaagttgaa gcacttgcag
     4261 atgatatcaa gttaattggc caagatcctt tttatcaaca tgttttagag gacaaacaat
     4321 ggacttatac ctattattaa aacgaaaaaa tcccaagctg cgtatgatat cgcaacttgg
     4381 gattttctgt attatctact ttgcaagtat gacattgggt ctactgcata ttgattaccg
     4441 atgccaccag acatacgttg gaagtgtacg tgaggcgctg ttgaattacc cgtactacct
     4501 gaatatgcaa tttggtcacc agctttgact ttatcaccag ctgaaacagt taaacgatta
     4561 ttatgcatat accattggta gttattactg ttcgcttctt taatcgttac ttgattacca
     4621 ccaccatagt tactccaacc tgcttgtact actgtaccat cagttaatga gtaaactggt
     4681 gaattttcag gcattgcata gtcgacaccg taatgcgcac caccaccgtg atattgtcca
     4741 tatggttgta gttgtttacg acttgttaac cagcttgcgt ctttcgcatg accactagct
     4801 gtcgctttac ttgctgatcc gccattttga ttagacgtgc cattagggta attgaccttt
     4861 ccattaccat catggctgtt atacgcttgg ttgttactat gtgaataata gctcgcgtct
     4921 ggacctacat ttgattgata accatattga ttaatgtgct gttggctttg actcgctgtg
     4981 tagtcattgt tatctcctgt tgttgctgga ttcacatatg tttgaccgct tccattggca
     5041 tttgcatttt ttggataaca gttataaaaa taatgcgtat gtccttgagc atctacgaat
     5101 gtatagctat attctttatt atcaaacatt gcttgattcc agttaccatc aggtgtgtga
     5161 tgataatccc cattagaatc aattgtataa taagtaccat aagatacgtc ttgtgattgt
     5221 gctgacattt gtgtatgtgc ttgttgggtg tttgtcgttt ctgctgcatc tgcttgatgc
     5281 gccattgtaa atgtagcgaa gcccatcgtt gcaatcgctg ctgctgttaa ttttttcatg
     5341 tataaaacat cctccattaa agttaaagtt agttttcaat taaactgtac tgcacatact
     5401 aaaagaatta gacaactgag taaaggattt aattctcatt ttccaactat ttaatattcc
     5461 cgaaatgttt tactaaactc attacattgt cattacaaaa tagccataca ttgatattaa
     5521 aatgacatct ctcactgcat tcgtttaacc ttttataaat tttcaaaact aacaactaat
     5581 cattcgtcca tgttcgcggt tcaacgctaa tgtatgatag taatcatcca tcaaatcata
     5641 tcaaccaaat tccattatca atcgctattg attgtcattc aactttctaa tagtgatatg
     5701 cttctcaggc ttaaaaatcg tcatatcttt tctattaatt aaatcatctg tgagctttaa
     5761 tgctactatt tcattgctac cataatactt aatatataac gttcttgtag ttaaatttat
     5821 tacggtctga tacatcgtat agtgattgtc agcatcatgc ggacgtacaa ttccaatcgg
     5881 tatatttacc gcatctaata aataaaatgc attcattaaa tccatttctt tatcattgtt
     5941 ttgagcaatg tttgctttca taaatgccat tctcacaaag cgctcagttg aagtaaatcc
     6001 acctggcaat ccaaatgtac ctgcttcatt gcctaaaggt tcaatcgtta caccttccaa
     6061 taaatttgct gttgctggat aaggagaaat attgatatat tgccttaaat tactataatg
     6121 ccaatctaag tctggatgat ttgttaagac accaataggg ttatctttta taaccacttc
     6181 accctcttta aatgaaactt cgactgattg tccagttgca tcggaaacat gataatgcaa
     6241 tggcggaact tcaccgatat catttaaata tacagctaca acatgtattt gggatgcttg
     6301 ttgtttcata tcttcaatgc ttgttgtata tcccaaaatc catgttacaa tttcattttg
     6361 cgtaatattc atcgcgtccg ctttgtgtgt tgatccatat gaactataac ctcggaaata
     6421 ttgtgttgaa atggcaacgc catgttcatt aacaccatca ccataaataa aaccttccat
     6481 atctgttcct gtgccaataa agccatattg cgtttggcct gtcgtgccag tgcgagatgt
     6541 ccaacgataa tttctaggcg tcactgctgg cgaaccatct aatggataat cataatccat
     6601 cgtgcgtcca agaagtactt gattatttaa agtttgtatt gtgaatcctg tgcacattgt
     6661 tctcactcct ctgtaccttc atttacttta atcactttca aataaagctg tttcacttaa
     6721 acatactata aaaaatcaat catacaagca attaattgat attcattctc aataactgtg
     6781 gtatgatatg taaggaaatc atgacttatg tgtgagtgaa cgatcatcta tacatccgtt
     6841 cacttcatct catgactttc tatatttaat ttttacaagg agtgacatct gtgaataaca
     6901 cacaatcttc accacgcagt aatattatta ttgcgattat gttgtctgca ttaacatatt
     6961 ggttgtttgc acaatcattt attaatatag gacctctcgt tggtcaaacg tatcaaacct
     7021 ctcctgccgt gttaaattta tctattagtt taacttcctt cgccacaggt atcttcatgg
     7081 tggctgcagg tgatattgct gataaaatag gacaactgag aatgacatac atgggtctca
     7141 taatcagtat gtttgcatct cttctattaa ttatatcgga catcactgca ctgctcatca
     7201 tcggtagaat tttacaaggt ctatcagcag ctatcttgtt accttcaaca gttggcgttt
     7261 taaataatca atttaaagga gaacatttaa gacgagcgat tagttatcta atgattagta
     7321 ctgttggtgg catcggccta gctggtgtta tcggcggttt aatcgccaca aatttcggat
     7381 ggcaaacgaa tttcatcatt agtatagtca ttgctttcat tgccatattg cttctaaaag
     7441 gcacacctga aaaagtaagt caacatagcc atcgtcatcc attcgattac aaaggtatgt
     7501 cgattttcgc tgttatgatt ggtagcttta cattattgtt aacacaagga ttcgaacaag
     7561 gttggtttag tacattttca ttcatttgtc tgagcatttt tatcatcact acgttgatat
     7621 tcatcatcat cgaacgtcga catgaagtac cttttattga tttttcagta ttacgcaacc
     7681 gtccgttcat tggtgcattt ttaaataact ttgttttaaa tagcggtcta ggcgtaacag
     7741 tggtcttttt catatatgct caaacacacc ttggtttatc agctgcgcaa tctggacttg
     7801 ttacattgcc atatgccatt gtggcagttg cgatgattcg tttaggtgaa aaagcaacat
     7861 tacgtttcgg tggcaaattg atgctcatca ttggtccgtt gttccctgtc atcggcatca
     7921 ctattattag catgactcaa ttatcggcat cacaatatgt cattgcagtt atcattggtt
     7981 tcgtcatttg cgcaattgga aatggtttag tcgcaacacc cggccttaca attgcaattt
     8041 tcagtatgcc aaatgaaaaa gttggtttag ctacaggatt atataaaatg agtggtacat
     8101 taggtggcgc ctttggtata gcactaagta ctacagtttt cagtatgtta caactaaact
     8161 atgcaccaag tgtagctgca accgtaacat ttatcgtcag cattgtattg atgatccttg
     8221 gctcattgtc tgcatacatg atcattccaa aaacagttaa atcttaaata taatagaaga
     8281 attatgtttc gaaatatctt tatcacttta aaatgatata caagaaattc aagaaaaata
     8341 agcgaagaac gtttttctct gttcttcgct tactctaatt atattcacta tcaattagac
     8401 aaaatggcca ttttcaaata tcacgcgttg tttctgacct tgaatatatt tattataatt
     8461 ctctttttga aaatcagtta acattaattt agatgtaccg tattttaaca ctttttgcat
     8521 tgtttctatt ctcatttttc taaataacca tccatctttt aacacaatac gattaacagc
     8581 atcatatgat aattctactg tttctttaat ttcaaatgtc ttgaatgaaa taatcgtgca
     8641 cattaaaaac gtatcaccaa agtaataaac atctaaatca tcacgtttat gttgtccaac
     8701 taacaaacga ccatattcga actctttttc tggatatttc aattctaaaa aactaataat
     8761 ctcttcttct tttaatttga attgcattta aaaacatcct ctcttaagtt ttaacaagcc
     8821 ttaattaaaa attttttcaa tcacatagtt caatatacat catttcgtta tattttttaa
     8881 tacttttttt caaaaacaaa tattttattc tttaaaataa tgacttttgt attcctcatc
     8941 acaataaaca ttttaaaatt ctagttatca taatcattca aaggtattaa ccttaataat
     9001 atattctctc gcctcaacct taatcgcata ctataggcgt ctgtttgtag acaataaaag
     9061 tcattcacgt cttcatatgt catcaaatgt ttatcatgat atgatgaata taataatcgg
     9121 gtatataact gtatgattaa ttacacaata aagagaagga atataatatg aaaaaagtgt
     9181 caattaaaga tgttgctaga gaagctggtg tatcagttac aactgtgtca catattttaa
     9241 atcataatga tagtcgtttt tccgcaacaa cgataaaaaa cgtacatgct gtttcagaac
     9301 gtttaggcta tgcccctaat aaacatgcaa aacaattgcg cggcagtaaa attcaaacta
     9361 ttggcgtcat tttacctagc ttaacaaatc cgtttttctc agcactaatg caaagtattc
     9421 atgaccataa accatctgat gttgatttat gctttttaac atctacagca actgatttgt
     9481 atgacaatat taaacattta attgatcgag gtattgacgg attaattatc gcacaataca
     9541 tatcatcccc ggacgcccta aataactatc taaagaaaca tcatgtacct tatgtcgtac
     9601 tggatcaaaa tgaccatcaa ggctatacag attttgttcg gacaaatgaa tatcaaggtg
     9661 gacaacttgc agcacaacat ttaatagaac tcggtcacaa caatatgata gttgttgcac
     9721 catatgacat gatggcgaat atgtcgactc gtgtcgctgg atttgtcgat actttgcgcg
     9781 cgaatcaatt gccagaacca caaatcgtcc atactgaatt atctaagcgc ggtgggctaa
     9841 ccattgttga tgacatcatg gttcaatctg ccactgcaat cttcgctatt aacgatgaac
     9901 tcgctattgg cattttacga ggactaattg aacatggcat cagtatcccg aaagatatct
     9961 cattaatagg ttatgacgac attgattatg cagcgtacgt ctcgccacct ttaactactg
    10021 tggcacaacc tataactgat attggcaaaa catctttaac cttattactt caacgattac
    10081 agcacttaga taaatccatt gatatgattg aattaccaac gactttaaaa attcgtgcaa
    10141 caactggcta tcatctttca aactaactac gtatcttccg aaatatactc atcattgtta
    10201 ggcccttagc gttgctttaa tgctgagggt ttttaatcat aattatttca ctaagaaatt
    10261 aaaataataa tgtatgaatt tttaaatatg atttaaacgt tttcagcttt tatgaaaacg
    10321 catgcatttt acaaataaaa atggtacgat ggcactggta aaacgtttta ctaaaaacaa
    10381 atcgtgaggt gtataacatg agcattgttg cattacttat cgggttaggc cccttaattg
    10441 gctggggatt cttcccaaca gtcgcttcaa agtttggtgg taaacctgta catcaaatta
    10501 tcggtgctac tgtaggtacg ttaatcttcg ctattatatt agccgtagtc acatcaagcg
    10561 gcttccctac tggaaccaat ttgctattcg ccttattatc aggtgctggt tggggattcg
    10621 gacaaatcat tacatttaaa gcgttcgaat tagtcggttc atctcgtgcc atgccagtca
    10681 caacagcatt ccaattatta ggcgcatctt tatggggtgt ctttgcatta ggaaattggc
    10741 caggcattgg tcataaaatc attggattta cagctttagt cgttattcta attggtgcgc
    10801 gtatgacagt ttggagtgaa cgcaaagaag caagtaacgc caaaaattta cgtcgtgcag
    10861 tggtacttct gttaattggt gaatttggat actggttata ttcagctgca ccgcaagcaa
    10921 cttctattga tggcctaact gcctttttac ctcaagcaat gggtatggta attgttgcag
    10981 tcatttatgg ctttatgaat atgaaagcag agaatccatt ccgtaataaa attacgtggt
    11041 tacaaattat ttcaggtttc ttctttgcat ttggtgcctt aacatatctt atttcagcac
    11101 aacctaatat gaatggttta gcaactggat ttattctttc tcaaacatcc gttgtgcttg
    11161 ctacattaac tggtatttat ttcttaaaac aacataaaac gtcaaaagaa atggttatta
    11221 caatcatcgg cttagtactc attttagtag ccgcttctgt tacagtattt ataaaataag
    11281 gagtgtagat gtcatgaaaa aaacagctgt tttaaatgaa catatttcaa aagcaatcgc
    11341 gacaattggt cattttgatc tattaacgat taatgacgct ggcatgccaa ttccaaatga
    11401 tcatcgtcgt atcgacctag ctgtaactaa aaacttacca cgctttattg atgtcttagc
    11461 tacagtgtta gaagaaatgg aaatccaaaa aatatactta gcagaagaaa taaaagaaca
    11521 taaccctaca caattgcaac aaattaaaca attgatttca tcggaaatcg aaatcatttt
    11581 cattcctcac gaagaaatga aaagtaactt agctcaccca ttaaataaag gtaatattcg
    11641 tactggtgaa acaacgccct actctaatat tgcattagaa tcgaatgtta ctttttaaaa
    11701 gttataactt gaaaggagcg tacacatgac caacaaagtt gttattttag gttcaacgaa
    11761 tgtcgatcaa tttttaacag ttgaaagata tgcacaacca ggcgaaacat tacatgttga
    11821 agaagcacaa aaagcattcg gcggaggtaa aggtgccaac caggctattg ccactgcacg
    11881 catgcaagca gacacaacat ttattactaa aattggcact gatggcgttg ctgatttcat
    11941 cttagaagat tttaaagcgg ctcatattga tacatcatat attatcaaaa cagctgaggc
    12001 aaaaacgggc caagccttta tcactgtgaa tgcagaagga caaaacacca tctatgttta
    12061 tggtggtgcg aatatgacga tgacacctga agatgttatt aacgcaaaag acgctataat
    12121 caatgcagac tttgtcgttg cacaattaga agtacccatc ccggctatta tatctgcatt
    12181 tgaaattgcc aagtcacatg gtgtgacgac agtattaaat cctgcaccag cgaaagcatt
    12241 acctaatgaa ttattgtcac taatcgatat tattgtgcca aacgaaacag aagccgaatt
    12301 gttatctggg attaaagtaa ctaatgaaca atctatgaaa gacaatgcta attacttttt
    12361 atctttaggc attaagactg ttttgattac gttaggtaag caaggtacat attttgctac
    12421 taaaaatcaa agccaacaca tcgaagctta taaagtaaat gcgattgata caactgctgc
    12481 aggcgacaca tttattggtg catttgtcag tcgcttaaac aagtcgcaag ataacttagc
    12541 agatgctatt gattttggta ataaagcgag ctcactcact gtacaaaaac acggcgcgca
    12601 agcatctatt cctctactag aagaagtaaa tcaagtttaa atgaatcaaa cacagctatg
    12661 atttgaaggt ttagcatata acatgcaaca ttcgtatatc atggctgtgc tttttatctt
    12721 tataaaacat catctactag aaataatttt ccactaaacc tatgcttgat gactcatgtt
    12781 tagttataaa tgaagtgaca attttttgtc atctgtttaa ctttcaaatt atcgcatata
    12841 aatatgctat attaatgata atgattatca attaataagg aggttatgtt atgtctaaag
    12901 aagccggtca tacattttta gctaaattag gaaaaactcg tctacgcccc ggtggtaaag
    12961 aagcaacaga ttggttaata caacaagggg cattttcaca agataaacaa gtgttagaag
    13021 tggcatgtaa tatgtgtacg acatctattt atctagctca tacatatggc tgtcacattc
    13081 aaggcgttga tataaataag aaagcattag aaaaagcaca agaaaatatc tcagcagcag
    13141 gtcttgaatc atatattcaa gttcaacaag cgaatgctgt taaattgccc tttgatgaca
    13201 atcaattcga tatcgtttta aatgaagcaa tgttaacaat gttacccatc gccataaagg
    13261 aaaaagcatt acgcgagtac taccgagtct taaagcctgg gggtatcttg ttaacacatg
    13321 atattgtcat cgttaatgaa tcacatgcca cacatgtagt taaatcatta tctgcagcaa
    13381 ttaatgtcaa tgtctcaccg cagacgaaac ttggctggtt agatttatat catcaagctg
    13441 gttttaatca tgtacattat catactggtc caatgagttt aatgacacct aaaggtttaa
    13501 tttatgatga aggtattgtc ggaactttaa agattatcaa caatgcttta aaaaaagaaa
    13561 atcgaccaat gttttgtaaa atgtttaaaa cgatgactaa actgcgaaaa gatatgaatt
    13621 atattacttt tgtcgctaaa aaagagcact aaatataatg ccactaactg tactttatat
    13681 ctatgtttga ctgttacttt aatttctttg tgacactaat catctactta acaatatcgt
    13741 tatcgttgat tagtaggtca tcaattttgg ttaaagactt ttataaacac tcaaacatta
    13801 acactataca tacttagtta gtggcattat ttttttctta aaattttaac atcacgggat
    13861 tggaacagaa atgatatttt cgcaaaattt atttatgtcc ctccccaact tgcacattat
    13921 tgtaagctga ctttccgcca gcttctatgt tggggccccg ccaacttgca ttgcctgtag
    13981 aatttctttt cgaaattctc tatgttgggg ccccgccaac ttgcacatta ttgtaagctg
    14041 actttccgcc agcttttgcg ttgggggccc gccaatacaa cactttaggt cctactatat
    14101 taatttatgt ctcgggctct aactctaaaa tacttataat atacctttat aatttatctc
    14161 catttgatgc tatcacttgt ttataccaat agaacgaatc ttttttcaag cgttccatcg
    14221 tgccatgacc atcattatct cgatcaacat atattaaacc atagcgtttc ttcatttcac
    14281 cagttgtaaa tgaaacaata tcaatgattc cccacggcgt ataaccgatt aaatctacac
    14341 catcttgatc aactgcttct atcgctgctg taatatgtac ttttaaatat tcaatgcgat
    14401 aatcatcatg aatatgtcca tctaccactt catcaactgc accaaaacca ttttccacaa
    14461 taaaaagtgg caactgataa cgatcatata acacattcaa tgtatatctt aagccatctg
    14521 gatcaatcgc ccaaccccaa tcactcgttg cgatatgcgg attctccaca gaatgattca
    14581 aaccaccgtt gacgatgttg ttttcaactg tagtatcaac atcatgtttt acagccgtag
    14641 acatgtaata actaaagcca atataatcaa ccgtgccctg ctgtaaaata ctgtcgtccc
    14701 cttcttgcca tccaaaatca tatccttttc gtgccaacat ttttttagca tagcttggat
    14761 aataacctct tacttgaaca tccgggaaaa agaaacgtaa gcgattcgca atttgtgctt
    14821 ccatcatatc tttcggatga cacgaatatg gataaatggg tacatgtgaa atcattgttc
    14881 caatcttaaa tttcggatta atctctttac caagacgaac cgctaaagca ctggctaaaa
    14941 gttcatgatg tgctacttga tacaagactt cttcgggatt atcattttct gttaatgcta
    15001 ccccagaatt tgtccataaa aagataggat ttgatgtgtc catctgatta ttaatttcat
    15061 taaacgtcat ccaatatgtc actttatctt tatatctttc aaatacaaca cgcgcaaaat
    15121 gcacaaaata atcgacaact tctctatttc taaatccacc ataatgtttc gctaaatgaa
    15181 gtggcatctc aaaatgtgat aacgtcacaa caggttcaat accttgtgca attaattcat
    15241 caaagatacg atcataaaag gcaagtcctt cttcatttgg cacatcttca tccccattcg
    15301 gaaagatacg tgtccacgca atcgacgtac gtaaacattt caatcccatt tctttaaaca
    15361 aggcaatatc ttccttataa cgatgataaa aatcaatacc ttcatgattt ggataatagt
    15421 gatttggatc tatagattct gtaatctgac gtgctttgcc atgtgcacca ctcgtcataa
    15481 catcaattac acttaatcct ttgccacctt tatcatatcc accttcaaat tgatttgcgg
    15541 caagagcgcc accccacata aaattttgcg gtaattttgt cattattaca acacactcct
    15601 agtttttaaa tattttaaaa ataacatctt cagtcgtaat gatttcattc attgtcagtt
    15661 ctacttttcc taaatcggca gagttgctaa taacgacaat aacgtctgag ttataacctt
    15721 gttcctgaat atattgttga tcgaattgta acagtgtttg cccagcttca acacgatcat
    15781 tttgctttac aaagcacttg aatcctttac cttccaagtc aactgtattc acgccaatat
    15841 gaatgactat gtccacacct tcttctgaac gaataccgat agcatgttta gtaggtacaa
    15901 tcatagatat taaaccattg aacggtgcga ttactttgga ttcttcatga gctttgattg
    15961 ccaagccttc acctaccatt ttctctctaa aaattgaatc tcttacttct ttaagtaaaa
    16021 tacgacgacc agcgattggt gctttcacta aaatcggtgt tgtcactttt gcttgaggtg
    16081 tataacttgt atcttggcta ttaatattgt cagtattcgc accctcttca acattagcat
    16141 ccttaattaa tcgctgtaat tgctgataca cttcatcgac atttccgttt aattttatga
    16201 ctaactgcgc atcattttct gctacagatg tcacatcaac tatttgtttc acttcatttg
    16261 ctgtagaagg aattgtatgt tgcatatgaa ttgtaatgcc cttcgtgtca tacgttacat
    16321 caacaatgtt ttcgacacca cccacagctg taagaatgtc ttgggcttgg tctttatatt
    16381 tattactcat aaatattacc ccctcaactt gatagtagaa tgaaaatgta tttacttacc
    16441 tccaattata attgtaccgg ttcattttgt aaatgacgat acaattataa tattttgtgc
    16501 tataataatt acagacaaag tgaaatgagg ataaaatatt gttaaagtac gaacatattg
    16561 ctaagcaact taatgcgttt atacatcatt ctaatttcaa acctggtgat aagttgccaa
    16621 gtgtaacgca attaaaagaa cgttatcaag taagtaagag tactatcatt aaagcattag
    16681 gcttattgga acaagatggt ttgatctatc aggcacaagg tagcggtata tatgtgagaa
    16741 atattgcaga tgcaaatcgt atcaatgttt ttaagaccaa tggtttttct aaaagtttag
    16801 gcgaacatcg aatgacaagt aaggtgcttg tttttaagga gattgcaacg ccacctaaat
    16861 ctgtacaaga tgaactccaa ttaaatgcag atgataccgt ctactattta gagcgattaa
    16921 gattcgtgga cgatgatgtt ttatgtatcg aatattctta ttatcataaa gaaatcgtga
    16981 aatatttaaa tgatgatatt gctaagggct ctatcttcga ctatttagaa tcaaacatga
    17041 aacttcgtat tggtttttca gatattttct ttaatgtaga tcaactcaat tcaagtgaag
    17101 cggcattact acaattatct aagggcgaac catgtttacg ttatcaccaa acgttttata
    17161 caatgactgg caaacctttt gattcgtctg acatcgtatt tcattatcgt catgcacagt
    17221 tttatattcc tagtaaaaag taatacatac atcaaaacgt ctatattcca gttataaacc
    17281 ggagtataga cgttttataa gacataattt ctttaaaaac agtgagaagt acatttttaa
    17341 tcagaaaatt actttatact gttctttatg tttacatgat ctaagaactt atagtttgaa
    17401 tcctattgta taatatctct tcccctcaaa cgttaaaaca cactaaaatc actcggtaat
    17461 atgatgatct atactctgta ttttggtttt aagtgtctgt atccccttat aaaaataact
    17521 actaatataa tgacacctac tgtgaatata atgtcccaag gcattctccc ccacatgatt
    17581 gcttttacaa gtggttgttc ataaaatgac gttagtcttg aatgccaata accatgttct
    17641 aatgcatctt taagttggat ataacctact ggcattaatg taacgaacac catacctgcc
    17701 aagccaatat ttaaagccca acatgatatt ttaatccatt tttcaacttt cggtgtccaa
    17761 aattcttttt tagtaatatt tttaaaaaca tacaacgcaa ttgcaattga gaacatacca
    17821 tacacgccag ccatagaacc atgggcatgt gctgcagtcc attgcgtacc atgttcaaag
    17881 tagttaactg ctggtgtgtt aattaaaaag cctaatgctc cagcaccaat tgcattccac
    17941 aatcctgttg aagcaagaaa aataaatgta cctttgtaag gaaaagcgat gtcactattc
    18001 ttatataagc gatagtgtgt gtatgcttcc caaattaata aacaaagcgg cacgacttct
    18061 aatgctgaaa aacaagaacc cagtgctagc catatggaat gatcaccttg ccaatagtaa
    18121 tgatgaccca taccaacaat acctgtacct agtaataaga ttaattgaaa atataatgca
    18181 cgaatcgtcg tttcaatggt agttaatttc atattaacca ttaagaaacc aatcaacact
    18241 acggcaaatg cttcaaatat accttcaacc cataagtgaa caatccacca tcgccagtaa
    18301 tcggcaaacg tcacatgtga attcggcatt ataaataatg atgcaagata aaataacgga
    18361 attgcaattg ctccaacaaa caatagcgtc aataagtgac gtttatcatg attcacttgt
    18421 actttgttgc gaatagctgg taaaaaacct cgacaaagaa tgataaccca taagaccata
    18481 cctactataa ataatatttg ccaaaattta cctaattcta tatattccca accaaagtgc
    18541 ccgaatagcc accaatgaga atcaatccaa cctaaaatat gaccccattc tccaatcatg
    18601 ctgcccatca caactatgat taaggcccaa aacaagatat ctactaatac gccttgtttc
    18661 tttggttctt tgcccaatac tttaggtaca atgtatattc ctgctgctaa ccaagtcgta
    18721 gcaacccaga aaataactaa ttgcacatgc catgtcttcg caatattaaa tggaaagagc
    18781 ctttgtattt ctataccaaa aaatttattc tctacataat aatgtgccag taattcgcct
    18841 aataatattt gaaccaaaaa tagtaacatg acaacgacta aatatttccc aacttttcgt
    18901 tgactatcag tgattttacg atccacttca atctttggta gttcccgttc cgactcatac
    18961 gtcggcttca tgtcaaattg ataacgcttt tgaatatata taatgaatgc caagcctgca
    19021 atcagtaatg ttacagatat agcagtccat aaaatccctg cactaggcaa cgtatttcct
    19081 gcatccacat cgtatggcca attatttgta tatgaaaatg tttttccagg tctatctgtt
    19141 gacgatagcc aagcacccca gaaaaagaat tgacttaaat gttcaacttt atttcctgcg
    19201 accatataat catcattttg aaactgttta atcatatttt catttaaccc tacttgttta
    19261 gggttattaa caaactcttt tttataataa tcttgcaaat attttagacc gtatacttgt
    19321 cctggcgtta atacaagttg atcttctttt tcagaataac gattaatccg tatttctttc
    19381 atcactttat ctttaactac ggctaactct gcatgattta atttatgaag ttgcttatga
    19441 taaatatctt gtgcataata tttacgcatg ccaatcaggt aatgatgtaa tgcctctgct
    19501 gtataatctg gacctaaata ggaaccattg cctaggtagg agccataatc tgttaaccca
    19561 tatttctcat atatcgcttg cccactgatc agctcgcctt ttgtaacaag cgtatgacct
    19621 tgttgatcta ctatctttgt tggacgaggt gcttcatttt taaaaatata ccaaccacct
    19681 actagtaaaa ttgaaaacac aatcactaaa atcgttagta gcactttaac taaacgttta
    19741 tatgaacttt gcatactcat ttataaaatc tcctcttaat tgcattc
//
LOCUS       DAHHOJ010000047        19240 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.047, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000047 DAHHOJ010000000
VERSION     DAHHOJ010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19240
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.047"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..196
                     /locus_tag="NBH32_001415"
     CDS             <1..196
                     /locus_tag="NBH32_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000876216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MSA protein"
                     /protein_id="HCD1515018.1"
                     /translation="YNVHLMTLPKFNNYIKTEMMELEHIDVLITSKDFGFDEILFYTL
                     YLLLILIVLYYLKKQVKNKN"
     gene            complement(437..1702)
                     /gene="lysA"
                     /locus_tag="NBH32_001416"
     CDS             complement(437..1702)
                     /gene="lysA"
                     /locus_tag="NBH32_001416"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCD1515019.1"
                     /translation="MTVKYNQNGELTMDGISLKTIAQSFGTPTIVYDELQIREQMRRY
                     HRAFKDSGLKYNISYASKAFTCIQMVKLVAEEDLQLDVVSEGELYTALEAGFEPNRIH
                     FHGNNKTKHEIRYALENNIGYFVIDSLEEIELIDRYANDTVQVVLRVNPGVEAHTHEF
                     IQTGQEDSKFGLSIQYGLAKKAIDKVQQSKHLKLKGVHCHIGSQIEGTEAFIETAKIV
                     LRWLKEQGIQVELLNLGGGFGIKYVEGDESFPIESGIKDITDAIKSEIKVLGIDAPEI
                     GIEPGRSIVGEAGVTLYEVGTIKEIPGINKYVSIDGGMSDHIRTALYDAKYQALLVNR
                     NEKADDSVTIAGKLCESGDIIIKDAKLPSSVKRGDYLAILSTGAYHYSMASNYNQMQK
                     PSVFFLKDGKAREVIKRQSLRQLIINDIK"
     gene            complement(1692..2777)
                     /locus_tag="NBH32_001417"
     CDS             complement(1692..2777)
                     /locus_tag="NBH32_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCD1515020.1"
                     /translation="MTATWSVNKKIFLQNAITVKNNQPLMAVVKNNAYHYDLEFAVTQ
                     FIHAGIDTFSTTSLREAIQIRQLAPDATIFLMNAVYEFDLVREHQIHMTLPSLTYYYK
                     HKNDLAGIHVHLEFENLLHRSGFKDLNEIKEVLKDHHHNQNAKMIISGLWTHFGYADE
                     FDVSDYNVERSQWMEIVEALLSEGYQFDLIHAQNSASFYREGQILLPHHTHARVGIAL
                     YGSRPYSSLNQHDIVQSLTVKAHVIQVREVQAGDYCGYSFAFEVTKNNTKLAVVDIGY
                     GDGILRTRAKHEALINGKRYPIRALMMSHMFVEVDGNVHAQDEVILYNNDIRIDEYTF
                     KGVGANSEQLSAMNHDSLKKEYISNDC"
     gene            complement(2782..3933)
                     /locus_tag="NBH32_001418"
     CDS             complement(2782..3933)
                     /locus_tag="NBH32_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1515021.1"
                     /translation="MNELEFVTKHRRHLHQHPELSLHEFETTAYIKAFLDSLNIKYDC
                     PLETGVIAYLEGNGSHTIAYRADIDALPILEENDVPYRSQSDHVMHACGHDGHTTALM
                     LFVQRCKDMQDASQLPQNVVFIFQPAEETGGGANRLIKAGAFDKYPIEAVFGIHVNPF
                     ADEGIAVIRDEEITASATEYRFFLTGLSSHVADKEQGHSCGEALQHVLTQISQIQQFH
                     LNGLKRNIVHIGHFKAGEAINTVPSNGYLEGTIRTYDIDDLTIVKNQMHKIAESVKLL
                     FNVDCEVKFAEGYPPTINSPKLRTQIEDALIKADLNVYDKPTPFLFGEDFSFYGQQLA
                     PAYFVFIGTRNEDKGFVTGLHTSHLNFDEKVLINVVNFYENLLNNYKEV"
     gene            complement(4076..4795)
                     /gene="dapD"
                     /locus_tag="NBH32_001419"
     CDS             complement(4076..4795)
                     /gene="dapD"
                     /locus_tag="NBH32_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HCD1515022.1"
                     /translation="MVQHLTAEEIIQYISDAKKSTPIKVYLNGNFEGITYPESFKVFG
                     SEQSKVIFCEADDWKPFYEAYGSQFEDIEIEMDRRNSAIPLKDLTNTNARIEPGAFIR
                     EQAIIEDGAVVMMGATINIGAVVGEGTMIDMNATLGGRATTGKNVHVGAGAVLAGVIE
                     PPSASPVIIEDDVLIGANAVILEGVRVGKGAIVAAGAIVTQDVPAGAVVAGTPAKVIK
                     QASEVQDTKKEIVAALRKLND"
     gene            complement(4822..5544)
                     /locus_tag="NBH32_001420"
     CDS             complement(4822..5544)
                     /locus_tag="NBH32_001420"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000698231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCD1515023.1"
                     /translation="MKILLIGYGAMNQRVARLAEEKGHEIVGVIENTPKATTPYQQYQ
                     HIADVKDADVAIDFSNPNLLFPLLDEEFHLPLVVATTGEKEKLLNKLDELSQNMPVFF
                     SANMSYGVYALTKILAAAVPLLDDFDIELTEAHHNKKVDAPSGTLEKLYDVIVSLKEN
                     VTPVYDRHELNEKRQPQDIGIHSIRGGTIVGEHEVLFAGTDETIQITHRAQSKDIFAN
                     GAIQAAERLVNKPNGLYTFDNL"
     gene            complement(5541..6428)
                     /locus_tag="NBH32_001421"
     CDS             complement(5541..6428)
                     /locus_tag="NBH32_001421"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCD1515024.1"
                     /translation="MTHLFEGVGVALTTPFTNNKVNLEALKAHVNFLLENNAQAIIVN
                     GTTAESPTLTTDEKERILKAVIDLVDKRVPVIAGTGTNDTEKSIQTSIQAKALGADAI
                     MLITPYYNKTNQRGLVKHFEAIADAVKLPVVLYNVPSRTNMTIEPETVETLSQHPYIV
                     ALKDATNDFEYLEEVKKRIDTNVFALYSGNDDNVVEYYQRGGQGVISVIANVIPKEFQ
                     ALYDAQQSGLDIQDQFKPIGTLLSALSVDINPIPIKALTSYLGFGNYELRLPLVSLED
                     TDTKVLREAYDTFKAGENE"
     gene            complement(6430..7419)
                     /locus_tag="NBH32_001422"
     CDS             complement(6430..7419)
                     /locus_tag="NBH32_001422"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCD1515025.1"
                     /translation="MTKLAVVGATGLVGTKMLETLNRKNIPFDELVLFSSARSAGQEV
                     EFQEKTYTVQELTDARASEHFDYVLMSAGGGTSEHFAPLFEKAGAIVIDNSSQWRMAE
                     DIDLIVPEVNEPTFTRGIIANPNCSTIQSVVPLKVLQDAYGLKRVAYTTYQAVSGSGM
                     KGKKDLAEGVNGKAPEAYPHPIYNNVLPHIDVFLENGYTKEEQKMIDETRKILNAPDL
                     KVTATCARVPVQDSHSVEIDVTLDKEATAEDIKALFDQDDRVVLVDNPENNEYPMAIN
                     STNKDEVFVGRIRRDDSLENTFHVWCTSDNLLKGAALNAVQVLEQVMRLKGAN"
     gene            complement(7483..8688)
                     /locus_tag="NBH32_001423"
     CDS             complement(7483..8688)
                     /locus_tag="NBH32_001423"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD1515026.1"
                     /translation="MVTRSVLKFGGSSVSDFTKIKRIAEMLKERVNQDEQLIVVVSAM
                     GNTTDQLMTNVSTLTKAPKQQELALLLTTGEQQTVSYLSMVLNDIGVNAKAMTGYQAG
                     IKTIGHHLKSKIAQINPQTFEQAFQENDILVVAGFQGINEHQELTTLGRGGSDTTAVA
                     LAVSNQIPCEIYTDVDGVYATDPRLLPKAKRLDIVSYEEMMEMSALGAGVLETRSVEL
                     AKNYNIPLYLGKTLSNVKGTWIMSNEEILEKKAVTGVALDKHMMHVTISYPLPDNQLL
                     TQLFTELEEGAVNVDMISQIVNLDGLQLSFTIKDSDFHQISMILETLKNQYEALAYKI
                     NEHYVKISLIGSGMRDMSGVASKAFLTLIENNIPFYQTTTSEISISYVIDDFNGQQAV
                     EKLYDAFNI"
     gene            complement(9548..11149)
                     /locus_tag="NBH32_001424"
     CDS             complement(9548..11149)
                     /locus_tag="NBH32_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1515027.1"
                     /translation="MLQVTDVSLRFGDRKLFEDVNIKFTEGNCYGLIGANGAGKSTFL
                     KILSGELDSQTGHVSLGKNERLAVLKQDHYAYEDERVLDVVIKGHERLYEVMKEKDEI
                     YMKPDFSDEDGIRAAELEGEFAEMNGWNAEADAANLLSGLGIDPTLHDKKMAELENNQ
                     KIKVLLAQSLFGEPDVLLLDEPTNGLDIPAISWLEDFLINFDNTVIVVSHDRHFLNNV
                     CTHIADLDFGKIKVYVGNYDFWYQSSQLAQKMAQEQNKKKEEKMKELQDFIARFSANA
                     SKSKQATSRKKQLEKIELDDIQPSSRRYPFVKFTPEREIGNDLLIVQNLSKTIDGEKV
                     LDNVSFTMNPNDKAILIGDSEIAKTTLLKILAGEMEPDEGSFKWGVTTSLSYFPKDNS
                     EFFEGVNMNLVDWLRQYAPEDEQTETFLRGFLGRMLFSGEEVKKKASVLSGGEKVRCM
                     LSKMMLSSANVLLLDEPTNHLDLESITAVNDGLKSFKGSIIFTSYDFEFINTIANRVI
                     DLNKQGGVSKEIPYEEYLQEIGVLK"
     gene            11296..12198
                     /gene="cvfB"
                     /locus_tag="NBH32_001425"
     CDS             11296..12198
                     /gene="cvfB"
                     /locus_tag="NBH32_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding virulence regulatory protein CvfB"
                     /protein_id="HCD1515028.1"
                     /translation="MALDKDIVGSIEFLEVVGLQGSTYLLKGPNGENVKLNQSEMNDD
                     DELEVGEEYSFFIYPNRSGELFATQNMPDITKDKYDFAKVLKTDRDGARIDVGLPREV
                     LVPWEDLPKVKSLWPQLGDHLLVTLRIDRENHMYGRLASESVVENMFTPVHDDNLKNE
                     VIEAKPYRVLRIGSFLLSESGYKIFVHESERKAEPRLGESVQVRIIGHNDKGELNGSF
                     LPLAHERLDDDGQVIFDLLVEYDGELPFWDKSSPEAIKEVFNMSKGSFKRAIGHLYKQ
                     KIINIETGKITLTKKGWSRIDSKE"
     gene            12511..13494
                     /locus_tag="NBH32_001426"
     CDS             12511..13494
                     /locus_tag="NBH32_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCD1515029.1"
                     /translation="MKKWQFVGTTALGATLLLGACGGGNGGSGNSDLKGEAKGDGSST
                     VAPIVEKLNEKWAQDHSDAKVSAGQAGTGAGFQKFIAGDIDFADASRPIKDEEKQKLQ
                     DKNIKYKEFKIAQDGVTVAVNKENDFVDELDKQQLKAIYSGKAKTWKDVNSKWPDKKI
                     NAVSPNSSHGTYDFFENEVMNKEDIKAEKNADTNAIVSSVTKNKEGIGYFGYNFYVQN
                     KDKLKEVKIKDENGKATEPTKKTIQDNSYALSRPLFIYVNEKALKDNKVMSEFIKFVL
                     EDKGKAAEEAGYVAAPEKTYKSQLDDLKAFIDKNQKSDDKKSDDKKSEDKK"
     gene            13685..14611
                     /gene="pstC"
                     /locus_tag="NBH32_001427"
     CDS             13685..14611
                     /gene="pstC"
                     /locus_tag="NBH32_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HCD1515030.1"
                     /translation="MTSSTNVKALIEKNNNKKGKHNDKIIPVILAAISAISILTTLGI
                     LITLLLETITFFTRIPITEFLFSTTWNPTGSDPKFGIWALIIGTLKITVIATIFAVPV
                     GLGAAIYLSEYASDRARRIIKPILEILAGIPTIVFGFFALTFVTPVLRSFIPGLGEFN
                     AISPGLVVGIMIVPLITSLSEDAMASVPNKIREGAYGLGATKLEVATKVVLPAATSGI
                     VASIVLAISRAIGETMIVSLAAGSSPTASLSLTSSIQTMTGYIVEIATGDATFGSNIY
                     YSIYAVGFTLFIFTLIMNLLSQWISKRFREEY"
     gene            14613..15530
                     /gene="pstA"
                     /locus_tag="NBH32_001428"
     CDS             14613..15530
                     /gene="pstA"
                     /locus_tag="NBH32_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCD1515031.1"
                     /translation="MEMTDNNRQSLVDQQLVQKHLSSRTVKNKVFKLIFLACTLLGLV
                     VLIALLTQTLIKGVSHLNLQFFTNFSSSTPSMAGVKGALIGSLWLMLSIIPLSIILGI
                     GTAIYLEEYAKNNKFTQFVKISISNLAGVPSVVFGLLGYTLFVGGAGIEALKMGNSIL
                     AAALTMTLLILPIIIVSSQEAIRAVPNSVREASYGLGANKWQTIRRVVLPAALPGILT
                     GFILSLSRALGETAPLVLIGIPTILLATPRSILDQFSALPIQIFTWAKMPQEEFQNVA
                     SAGIIVLLVILILMNGVAIILRNKFSKKF"
     gene            15577..16428
                     /locus_tag="NBH32_001429"
     CDS             15577..16428
                     /locus_tag="NBH32_001429"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HCD1515032.1"
                     /translation="MAQTLAQTKQISQSHTFDVSQSHHKTPDDTNSHSVIYSTQNLDL
                     WYGENHALQNINLDIYENEITAIIGPSGCGKSTYIKTLNRMVELVPTVKTAGKILYRD
                     QDIFDQKYSKEQLRTNVGMVFQQPNPFPKSIYDNITYGPKIHGIKNKKVLDEIVEKSL
                     RGAAIWDELKDRLHTNAYSLSGGQQQRVCIARCLAIEPEVILMDEPTSALDPISTLRV
                     EELVQELKEKYTIIMVTHNMQQAARVSDKTAFFLNGYVNEYDDTDKIFSNPSNKKTED
                     YISGRFG"
     gene            16435..17076
                     /gene="phoU"
                     /locus_tag="NBH32_001430"
     CDS             16435..17076
                     /gene="phoU"
                     /locus_tag="NBH32_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCD1515033.1"
                     /translation="MAIIRQRYQEQLDDLIKELRRLGANVYVSIENGIKSLSIDDRGF
                     ARQTVKNDKHINQLNYNINERVIMLITKQQPIASDLRMMISSLKIASDLERIGDNASS
                     IANIRLRTKITDDYVLTRLKTMGKLAMLMLKDLDQAFKKKDTVLIREIIERDEDIDDL
                     YSHIINATYLIDNDPFVAAQAHLAARHLERIGDHIINIAESVYFYLTGTHYEQ"
     gene            complement(17219..19033)
                     /gene="pepF"
                     /locus_tag="NBH32_001431"
     CDS             complement(17219..19033)
                     /gene="pepF"
                     /locus_tag="NBH32_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD1515034.1"
                     /translation="MSQGLLLREDVPVSETWDLVDLFKDDQQYYESIDALVQQANQFH
                     HTYATTLNSIEQINTALAELENILIALDRLSNYAELRLSVDTSNIEAQVLSAKLSTTY
                     GKIVSQLSFVESEILELPEEILQQLEESCPYQHYIKQLIKQKPFQLSASVEQVLATLS
                     PTLNSPYDLYGTTKMLDITFDSFEHDGTTYPVDYATFENDYEDNKDPEFRRKSFKSFS
                     DGIRKYQHTTAATYNMQVQQEKIEADLRGFESVIDYLLHSQEVTRDMFDRQIDMIMRD
                     LAPVMQKYAKLLQRIHELDNMRFEDLKISVDPDYEPEISIEDSKNYIFGALSVLGDDY
                     TNMLREAYDQRWIDFAQNKGKDTGAFCASPYFTHSYVFISWTGKMAEAFVLAHELGHA
                     GHFTSAQKHQPYLESEASMYFVEAPSTMNEMLMANYLFNTSDNPRFKRWVIGSILSRT
                     YYHNMVTHLLEAAYQREVYHKVDQGESLNAPTLNEIMLNVYKQFFGDVVDMTEGAELT
                     WMRQPHYYMGLYSYTYSAGLTIGTVVSQKIKNEGQQAVDAWLETLKKGGSVSPVELAN
                     IAGVDITTEQPLKSTIQYISDLVDEVEKLTDEIEQANN"
BASE COUNT         6275 a         3329 c         3105 g         6531 t
ORIGIN      
        1 ttacaatgta catttaatga ctttgccaaa gtttaataat tatatcaaaa cagagatgat
       61 ggaattagaa catattgatg tattaattac aagtaaagat tttggatttg atgaaattct
      121 gttttataca ttatatttgt tgttaattct tatcgtttta tattatttga aaaagcaagt
      181 aaagaataaa aactaatttc atgatgcttg tttaaagtgt ggtatgtaaa tgatgcttta
      241 aattagttga atagggctaa ctaaatataa aggattaatg acaaaaagaa ataaatactt
      301 tctaactaaa aaagtgctgt tcttcctttt ttagagtata tgtccaagca tgttatctac
      361 agtcatccta taatgcataa aaattccccg agtcacagta taattttctg tgcttcgggg
      421 attactttgt ttatttttat tttatatcat taataatgag ttgtcttaac gattgtcgct
      481 ttataacttc acgtgctttg ccatctttta agaaaaacac agaaggcttt tgcatttgat
      541 tgtaattgga tgccatagag taatgatatg caccagttga taatatagca agatagtctc
      601 cacgtttgac tgatgaaggt aatttagcgt ctttaataat gatatcacca gactcgcata
      661 attttccagc tatagttaca ctgtcatctg ctttttcatt tctattaaca agcaatgctt
      721 gatactttgc gtcataaagt gcagttctga tatgatcact cataccgcca tcgatagaaa
      781 catatttatt aatccctgga atttctttaa tggttccaac ttcatataaa gtaacgccag
      841 cttcacctac aatagatcgt cctggttcaa tacctatttc tggtgcatct atacctagaa
      901 ctttaatttc ggattttatt gcgtctgtaa tatctttaat accactttcg atagggaaac
      961 tttcatcacc ttcaacatat ttaataccaa agccaccacc aaggtttaat aattcaactt
     1021 gaatgccttg ctctttaagc caacgtaaaa caattttagc agtttcaata aatgcttctg
     1081 taccttcaat ctgtgaacca atatgacaat gtacaccttt taattttaag tgtttagatt
     1141 gttggacttt gtcaattgct tttttagcta agccatattg aattgataac ccaaacttac
     1201 tatcttcttg accagtttga ataaattcgt gtgtatgtgc ttcaacacct ggattaactc
     1261 gtaatacaac ttgaaccgta tcattagcat agcggtctat taattcaatt tcttctaatg
     1321 aatctataac aaaataaccg atgttatttt ctaaagcata cctaatttca tgtttcgttt
     1381 tattgttacc atggaaatgg atgcgattcg gttcaaaacc tgcttctaaa gctgtatata
     1441 attcaccttc agaaacaaca tctaactgta aatcttcctc agctacaagt ttgaccattt
     1501 gaatgcaagt aaatgccttt gaggcgtatg aaatattgta ttttaatcca ctatctttaa
     1561 atgcgcgatg gtaacggcgc atctgttctc taatttgtag ttcatcataa acaatggtag
     1621 gtgtaccaaa gctttgcgca atcgttttta aactaatacc atccattgtt aattcgccat
     1681 tttgattata tttaacagtc atttgaaatg tactcctttt ttaaagaatc atgattcata
     1741 gcacttaatt gttcagaatt tgcaccaaca cctttaaagg tatattcatc gatgcggata
     1801 tcattattat aaagaataac ttcatcttgt gcatgtacat tgccatctac ttcaacaaac
     1861 atatggctca tcattaatgc acgtatcggg tagcgtttac cattgataag tgcttcatgt
     1921 ttagcacgag ttcttaaaat tccatcgcca taaccgatat caactacagc taattttgta
     1981 ttgtttttag tcacttcaaa ggcaaagcta taaccgcaat aatcaccagc ttgtacttcg
     2041 cgcacttgaa taacatgtgc ttttacagtt aatgactgaa ctatatcatg ttgattcagt
     2101 gaactatatg gtcttgaacc gtataacgca atacctacac gcgcatgtgt atggtggggt
     2161 agtaatattt gtccttcccg ataaaaactc gcactatttt gagcatggat taggtcgaac
     2221 tgataacctt cagataaaag tgcttcaaca atttccatcc attgtgaacg ttcaacatta
     2281 taatctgaca catcgaattc atcagcatat ccaaaatggg tccataaacc actaataatc
     2341 atttttgcgt tttgattatg atggtgatct ttcaatactt ctttaatttc gtttaaatct
     2401 ttaaatccag accgatgtaa taaattttca aattctaagt gaacatgaat acctgctaaa
     2461 tcatttttat gcttatagta atatgtcaac gacggcaaag tcatgtgtat ttgatgttca
     2521 cggactaaat caaactcgta aactgcattc attaaaaaga ttgttgcatc tggagcaagt
     2581 tgtctaattt gaattgcttc tcgtagtgat gttgtgctaa atgtatctat acctgcatgg
     2641 ataaactgag ttacagcaaa ttctaggtca tagtgatatg cattattttt aacaactgcc
     2701 attaatggct gattgttttt gactgtgatt gcattttgta aaaatatttt cttatttaca
     2761 gaccatgttg ctgtcaatgt attacacctc tttgtaatta tttaataaat tttcgtaaaa
     2821 attaaccacg tttattaaca ctttttcatc aaaatttaaa tgtgatgtgt gcaaaccagt
     2881 tacaaaacct ttatcttcat ttcgtgttcc tataaaaaca aagtaagctg gagctagttg
     2941 ttgaccataa aaactaaaat cttccccaaa taagaatggc gttggtttgt catagacatt
     3001 taaatcagct tttattaagg cgtcctctat ttgagtacgt aatttcggac tattgattgt
     3061 agggggataa ccttctgcaa atttaacttc acaatctaca ttaaacagaa gcttgacact
     3121 ttctgctatc ttgtgcattt gatttttaac gattgttaaa tcatcaatat catatgtacg
     3181 aatagtacct tctaaataac cattacttgg tacagtgtta atcgcttcac cagctttaaa
     3241 atgaccaata tgaacaatat ttcgtttcaa accgttaagg tgaaattgtt gaatttgtga
     3301 tatttgagtt aatacatgtt gtaatgcttc accacaagaa tgaccttgtt ctttatcagc
     3361 aacatgactt gacaggcctg ttaagaaaaa gcgatactct gttgcgctgg ccgtaatttc
     3421 ttcatctctt atcactgcaa tgccttcatc agcaaatggg ttaacatgaa taccaaatac
     3481 cgcttcaatt ggatacttat caaaggcacc ggcttttatt aatcgatttg caccgccacc
     3541 agtttcttct gcaggttgga aaatgaaaac gacattttgc ggtaattgac ttgcatcttg
     3601 catgtctttg caacgttgta caaaaagcat taatgcagtt gtatgaccat catgtccaca
     3661 agcatgcatc acatgatcag attgactgcg ataaggcaca tcattttcct ctaaaatagg
     3721 taacgcatca atatcagctc tatacgctat cgtatgtgag ccattacctt ctaagtatgc
     3781 aatgacgcca gtttccaatg ggcaatcgta tttaatattt aaactatcta aaaacgcttt
     3841 aatataagca gttgtttcaa attcatgtaa gcttaattca ggatgttgat gtaaatgacg
     3901 gcgatgtttc gtaacaaatt ctaattcatt cataattatc aatcctttgt gttaaattac
     3961 tatataaata gtgtaacgta tttcgaaatt tgtgatcata agtttattca atgataaaca
     4021 ataaggttga gacataatcg tatctcaacc ttgaaattat tatacgttga cgtcactagt
     4081 catttagttt tcttaatgct gctacaattt cttttttagt atcttgtact tcagaagctt
     4141 gcttaatcac ttttgcaggt gtaccagcaa caactgcacc agctggtaca tcttgtgtca
     4201 caatcgcacc agctgcaaca atagcacctt taccaacacg tacaccttct aaaataactg
     4261 catttgcacc gattaaaaca tcatcctcga ttataactgg tgaagcacta gggggttcaa
     4321 tcacacctgc taatactgcg ccagccccta catgtacatt tttaccagtt gtagcacgac
     4381 caccgagagt agcattcata tcaatcattg taccttcgcc aacgactgcg ccaatattaa
     4441 ttgttgcgcc catcataacg acagcaccat cttcaataat ggcttgttct ctaataaacg
     4501 cacctggttc aattcgtgca ttcgtatttg ttaagtcttt taatggaatg gcagaattgc
     4561 gacgatccat ttcaatttct atatcttcga attgactacc atatgcttcg taaaaaggtt
     4621 tccaatcatc tgcttcacaa aagattactt ttgattgttc tgaaccaaat actttaaaac
     4681 tttctggata tgtgatgcct tcaaaattcc catttaaata tactttaatt ggtgtagatt
     4741 ttttagcatc acttatatat tgaataattt cttcagctgt taaatgttgt accataaaat
     4801 aatcgatctc ctttaatatg tttataggtt gtcaaacgta tataagccgt ttggtttatt
     4861 aactaagcgt tctgctgctt gtattgcacc attcgcaaaa atatcttttg attgtgcacg
     4921 atgcgtgatt tgaatcgttt catcagtgcc ggcaaataga acttcatgtt caccgacaat
     4981 cgtacctcca cgaatagaat gtataccaat atcttgtggc tggcgttttt catttaattc
     5041 atgtctatca tacacaggtg ttacattttc tttcaaagat acaatcacgt catataattt
     5101 ttctaacgta ccacttggtg catctacttt tttattatga tgtgcctcag tcaattcgat
     5161 atcgaaatca tcaagtaggg gaacagcagc tgctaaaatt ttagtcaatg cataaacgcc
     5221 ataactcatg ttcgcgctga aaaatacagg catattttga ctcaattcat ctaacttatt
     5281 aagtagtttt tctttctcgc cagttgttgc taccactaat ggcaaatgaa actcttcatc
     5341 taataatggg aataatagat tcggatttga aaaatctatt gcaacatcgg catctttaac
     5401 atctgcaata tgttgatatt gttgatatgg cgttgttgct ttcggtgtat tttcaatgac
     5461 cccaacaatt tcatggcctt tttcttctgc taatctagca acgcgttgat tcattgcgcc
     5521 atagccaatt agtaatatct tcactcattt tcacccgcct taaatgtgtc atatgcttca
     5581 cgaagcactt tagtatctgt atcttctagg ctaaccaatg gtaaacgtaa ttcatagttt
     5641 ccaaatccta aataacttgt taaagcttta ataggaattg ggttaatatc aactgatagg
     5701 gctgataaca gtgtgccgat tggtttaaat tgatcttgaa tatctaatcc gctttgttga
     5761 gcatcgtata acgcttgaaa ttctttagga atgacattag caatgacaga aataacgcct
     5821 tgaccaccac gttgatagta ttctacaaca ttgtcatcat tgccactata taaagcaaat
     5881 acattggtat caatgcgctt tttcacttct tctaaatact caaaatcatt cgtagcatct
     5941 tttaaagcaa ctatataagg atgttgactt aatgtttcga ctgtttctgg ttcaatcgtc
     6001 atatttgttc ttgatggcac attgtacagc acgactggta atttcacagc atctgcaatc
     6061 gcttcaaagt gtttgactaa accacgttgg ttcgttttgt tgtagtaggg cgtaattaac
     6121 ataattgcat cagcacctaa ggctttagct tggattgaag tttggattga cttttcagta
     6181 tcattagtgc cagttcctgc tatgacagga acacgtttat ctacaagatc aataactgct
     6241 tttagaatgc gttctttttc atctgttgtt aaagtagggc tctcagcagt agttccatta
     6301 acgatgattg cttgggcatt attttctagt aaaaaattaa cgtgtgcttt caaagcttca
     6361 agattaactt tgttatttgt aaaaggggtt gtaagtgcaa cgccaacacc ctcaaataaa
     6421 tgtgtcattt taattcgctc cttttaaacg cataacttgt tccaatactt gtacagcatt
     6481 taatgcagca ccttttaata aattgtctga tgtacaccat acatggaaag tattttctaa
     6541 tgaatcatct ctacgtatac ggccaacaaa cacttcatct ttattagtag aattgattgc
     6601 cattggatat tcattgttct ctggattgtc tactaaaaca acgcggtcat cttgatcaaa
     6661 taatgcttta atatcttctg cagtcgcttc tttgtcaagc gttacatcaa tttcaacact
     6721 atgactatct tgaacaggca cacgtgcgca tgttgctgtt acttttaagt ctggcgcatt
     6781 taaaattttt ctcgtctcat caatcatttt ttgttcttct tttgtatatc cattttctaa
     6841 aaacacatca atatgcggta acacattatt ataaattgga tgtggatatg cttctggtgc
     6901 tttaccattt acaccttcag ctaaatcttt cttacctttc atccctgaac ctgatacagc
     6961 ttgatatgtt gtatatgcca ctcgttttaa accataagca tcttgcaata cttttaatgg
     7021 cacaacagat tgaatcgtag agcagtttgg attggcaatg atacctcttg taaatgtagg
     7081 ttcattgact tccggaacga ttaaatcaat atcttctgcc atacgccatt gacttgaatt
     7141 gtctataacg attgcaccag ctttttcaaa gagtggggca aagtgttcgc ttgtaccgcc
     7201 accagcactc attaatacat aatcgaaatg ttcacttgca cgagcatcag ttaattcttg
     7261 tactgtatat gttttttctt gaaattcaac ttcttgccct gcagaacgtg ctgatgaaaa
     7321 taatactaat tcatcgaaag gaatattttt acgatttaat gtctccaaca tttttgtacc
     7381 tactaatcct gttgcaccca caactgctaa ctttgtcata acttgtcact ccattttata
     7441 ataatttcca atttttagaa tattttaaca atcattttac cattaaatgt taaatgcgtc
     7501 atatagtttt tctaccgctt gttgcccatt aaaatcatca atgacgtatg aaatacttat
     7561 ttcagatgtt gttgtttggt agaaaggtat attattttca attaatgtca aaaatgcttt
     7621 tgatgccaca cctgacatat cacgcatgcc tgagccaatt aatgaaattt tgacataatg
     7681 ctcattgatt ttataagcta atgcttcata ttgattcttt aatgtttcaa gaatcataga
     7741 aatttgatga aaatcactat ctttaatcgt gaaggatagt tgtagcccat ccaagttgac
     7801 gatttgtgaa atcatatcaa catttacagc accttcttca agttccgtaa atagttgggt
     7861 aagtagctga ttgtcaggta ggggataact aattgttaca tgcatcatat gtttatccaa
     7921 agccacacca gtaactgctt ttttctctaa tatttcttca tttgacataa tccatgttcc
     7981 tttcacgttc gataaagttt ttcctaaata taaagggata ttatagtttt tagctaattc
     8041 aacacttctt gtttcaagta caccagcacc taaagcgctc atttccatca tttcttcata
     8101 tgagacgatg tctagtcgtt tagcttttgg taaaagtctt gggtcagtgg catacacacc
     8161 atcaacgtcg gtataaattt cacaaggtat ttgattacta acagcaagtg ccacagcggt
     8221 cgtatcagaa ccgcctctgc ctaaagttgt taattcctga tgttcattga tgccttgaaa
     8281 tccagcaact actaaaatat cgttttcttg aaaggcttgt tcaaatgttt gaggattaat
     8341 ttgagcaatt ttacttttta aatgatggcc aatggtttta atacctgctt gatagccagt
     8401 cattgctttg gcattcacac cgatatcatt taataccatt gataaataag atacagtttg
     8461 ttgctctccg gttgtcaata ataatgccag ttcttgttgt tttggtgctt tagtcaaggt
     8521 cgatacattc gtcattaatt gatctgttgt gttacccata gcacttacaa cgacaattaa
     8581 ttgttcatct tgattgactc gctcctttaa catttcagcg atccttttta tttttgtaaa
     8641 atcactgacg gatgatccgc caaatttcaa cacacttctt gttaccatat aatcctccta
     8701 attgttagga agggcacaca ataataaaaa aagagcaagt tcggaatgcg aacttgctct
     8761 taatctatat acaagtgatg cactccatta tttttaaata atgacaaact ctcagctctt
     8821 aaccaaaaag tccaacaaat tataactgct attataattg cttcggcatc gcaccctttc
     8881 aaatttagct gttagcagac agtaatctaa actttactca tgattgatgc gcctcatcaa
     8941 aatattatta aattggtatg tgttcattat acatatgaca aatatgaatg taaaccgata
     9001 atttagattt tttggaataa cctgaaaatt caagttatag cgttgcttat attttaaaag
     9061 gtggtgataa tgagactttt tgaaaaataa aattcaaaat acttatagca tactcaatat
     9121 gcacattaaa taaatgtact cttttaatgc ggtgataact gtatttgtaa tttagagaag
     9181 gggtgttcac tgtgctgatg tgttaaaaaa taaaataaaa aggacacctc gatgctataa
     9241 atattagcat cgagacgccc agttaatgtc tattaaattg aatatagtct cggatatgaa
     9301 tcaatgccct aggccctgca atgttatatt gacagtagtt gactgaatga aaatgacttt
     9361 gtagctagct tttttcaatc cttgtcggtg ccacacatag agaaattgga ttcctaattt
     9421 ctacaaacaa tacaagttgc ggaataagtc ccaatataga aggtgacagt aagccaactt
     9481 acaataatgt gcaagttggt cgggcctcaa cacagagatt ttcgaaaaga aattctacat
     9541 attatgttta ttttaaaacg ccgatttctt gcaagtattc ttcatatgga atttcttttg
     9601 aaacgccgcc ttgtttattt aaatcgataa cacggtttgc aatcgtgttg ataaattcga
     9661 agtcataaga agtaaagatg atagaacctt taaatgattt aagaccatca ttgacagcag
     9721 taatactttc taagtctaag tggttagtag gttcgtcaag taaaagtaca ttcgcacttg
     9781 ataacatcat tttacttaac atacaacgta ctttttctcc acctgaaagc acactagctt
     9841 ttttcttaac ttcttcacca ctaaataaca tacgacctaa gaaaccacgt aaaaatgttt
     9901 ctgtttgttc atcttcagga gcatattgtc ttaaccaatc aacgagattc atatttacac
     9961 cctcaaagaa ctctgagtta tctttaggga agtaacttaa tgatgtagta acaccccatt
    10021 taaatgaacc ttcgtctggt tccatttcgc cagctaatat tttaagtaat gttgtttttg
    10081 caatttcact atctccaatt aaaatcgctt tatcatttgg attcattgtg aatgatacat
    10141 tatctaatac tttttcgccg tcaattgttt tagaaagatt ttgaacgatt aataagtcgt
    10201 taccaatctc acgctcaggc gtgaatttaa cgaaaggata tcttcttgat gatggttgaa
    10261 tatcatctaa ttcaattttc tcaagttgtt ttttacgact tgttgcttgt ttagatttag
    10321 aagcgttagc tgagaaacga gcaataaagt cttgtaactc tttcattttt tcttcttttt
    10381 tcttgttttg ttcttgagcc atcttttgag ctaactgact agattgatac caaaaatcat
    10441 agttaccaac ataaacttta attttaccaa agtctaaatc agcgatatga gtacatacat
    10501 tatttaagaa atggcggtca tgtgatacta cgataacagt attatcaaag ttaattaaga
    10561 aatcttctaa ccaactgatt gctggaatat ccagaccgtt agtaggctca tccagtaata
    10621 gtacgtctgg ttcaccgaat aaactttgcg ctaataatac tttaattttt tggttgtttt
    10681 ctaattcagc cattttttta tcgtgtaaag ttggatcgat acctaaacca gataaaaggt
    10741 tagctgcatc agcctcagca ttccaaccat tcatttctgc aaattcacct tcaagttcag
    10801 cagcacggat accatcttca tcactgaaat ctggcttcat atagatttca tctttttctt
    10861 tcataacctc ataaagacgt tcgtgacctt taattacaac atcaagcaca cgttcatctt
    10921 cataagcata gtggtcctgt tttaaaacag ctagacgttc atttttacct aatgaaacat
    10981 gtcctgtttg agaatctaat tcaccagata atatttttaa gaatgttgat ttacctgcac
    11041 cattcgcacc aattaatcca taacaattac cttctgtaaa tttaatattt acatcttcaa
    11101 atagtttacg atctccaaaa cgtaaactca catcagttac ttgtaacatg cattttctcc
    11161 tttttttcat tcgatattct aacggaagaa ttatatcata ttatcgtcac agtttcgacc
    11221 tcatataagt tgtaatgata gaatgactca cacatgttat aataataaag aatacaagaa
    11281 tcgaaggaga ataacatggc attagacaaa gatatagtag gttctataga attccttgaa
    11341 gtagtagggt tacaaggttc aacttacctt ttaaaaggac caaacggtga aaacgtaaag
    11401 ttaaaccaat cagaaatgaa cgatgatgat gaattagaag taggtgaaga atatagtttc
    11461 ttcatttatc caaaccgttc aggtgaatta tttgcaactc aaaatatgcc tgatattacg
    11521 aaagataaat atgactttgc taaagtactt aaaacggatc gcgatggggc acgtatagat
    11581 gttggattac cccgtgaagt gttagtacca tgggaagatt taccaaaagt gaaatcacta
    11641 tggccacaac taggtgatca tttgctagtc acattacgaa ttgaccgtga gaatcatatg
    11701 tatggacgat tagcgagtga atctgttgta gaaaatatgt ttacacctgt acacgacgat
    11761 aatttaaaaa acgaagtcat tgaagccaaa ccttaccgcg tattacgaat tggtagcttt
    11821 ttattaagcg aatcaggtta caaaattttc gtacatgaat cagaacgtaa agctgaacca
    11881 agattaggtg aatctgttca agttagaatt atcgggcata atgataaagg tgagttaaat
    11941 ggttcatttt taccacttgc acatgaacgt ttagacgatg acggccaagt catctttgat
    12001 ttactagttg aatatgatgg tgaattacca ttctgggaca aatcaagccc tgaagcgatt
    12061 aaagaagtat tcaatatgag taaaggttca ttcaaacgtg caatcggtca cttatataaa
    12121 cagaagatta ttaatataga aacaggcaaa atcactttaa ctaaaaaagg ttggagtcga
    12181 attgactcaa aagaataatc atttttacac gtatcgtagg atgcgtgttt tttatattca
    12241 atattaaatc ggatagatga agtagttttt taatttcatc tgtccatttt tttgtgtaca
    12301 tatttaaatt tcgataattt ttcaggaagc attttaattt tactaatgaa gcaatatttt
    12361 ttagattaac aaaaattaat atttacattt tcttaacaaa tttttatgta acatttacag
    12421 tttctaaaaa tgaggttaat aattcaaggt taagataaag atgtaatcaa tacaaatact
    12481 atttgttgtt catacaggga ggatatttca atgaaaaaat ggcaatttgt tggtactaca
    12541 gctttaggtg caacactatt attaggtgct tgtggtggcg gtaatggtgg cagtggtaat
    12601 agtgatttaa aaggggaagc taaaggtgat ggctcatcaa cagtagcacc aattgtggag
    12661 aaattaaatg aaaaatgggc tcaagatcac tcggatgcta aagtctcagc aggacaagct
    12721 ggtacaggtg ctggtttcca aaaattcatt gcaggagata tcgacttcgc tgatgcttct
    12781 agaccaatta aagatgaaga gaagcaaaaa ttacaagata agaatattaa atacaaagaa
    12841 ttcaaaattg cgcaagatgg tgtaacggtt gctgtaaata aagaaaatga ttttgtagat
    12901 gaattagaca aacagcaatt aaaagcaatt tattctggaa aagctaaaac atggaaagat
    12961 gttaatagta aatggccaga taaaaaaata aatgctgtat caccaaactc aagtcatggt
    13021 acttatgact tctttgaaaa tgaagtaatg aataaagaag atattaaagc agaaaaaaat
    13081 gctgatacaa atgctatcgt ttcttctgta acgaaaaaca aagagggaat cggatacttt
    13141 ggatataact tctacgttca aaataaagat aaattaaaag aagttaaaat caaagatgaa
    13201 aatggtaaag caacagagcc tacgaaaaaa acaattcaag ataactctta tgcattaagt
    13261 agaccattat tcatttatgt aaatgaaaaa gcattaaaag ataataaagt aatgtcagaa
    13321 tttatcaaat tcgtcttaga agataaaggt aaagcagctg aagaagctgg atatgtagca
    13381 gcaccagaga aaacatacaa atcacaacta gatgatttaa aagcatttat tgataaaaat
    13441 caaaaatctg acgacaagaa atctgatgat aaaaagtctg aagacaaaaa ataataagac
    13501 gcaatttcaa atgtgtcttg aaacatgatt ttgatggtga atcattattt agagtacaaa
    13561 gcttgattta tcgagacgct gattttgaca ttcagtaagt ctacaagctt atcaacttaa
    13621 aatagtggtt catcattatt ttacaaatct aattattttg ggagtaatag aaagaggttt
    13681 gattatgact tcatctacta atgttaaagc tttaatcgaa aaaaataata ataaaaaagg
    13741 gaagcataat gacaaaatta taccagttat tttagccgca atttcagcga tttccatttt
    13801 aacaacacta ggtatattaa tcacattgct tttagaaacc atcacttttt tcaccagaat
    13861 tccaataact gaatttctat tttctactac ttggaatcct accggttcag accctaagtt
    13921 tggtatctgg gcattgataa tagggacttt aaaaatcaca gttattgcga ctatatttgc
    13981 agttccagtc gggcttggtg cagcaattta tttaagtgaa tatgcaagtg accgtgcacg
    14041 acgcataatt aaaccgatat tagaaatttt agcaggaata ccaacaattg tgtttggttt
    14101 ctttgcatta acctttgtta caccagtatt aagatctttc ataccaggtc ttggagagtt
    14161 taatgctata agtcccggct tagttgtcgg tattatgatt gtccctctca tcacaagttt
    14221 gagtgaggat gcaatggcat ctgtaccaaa taaaattcga gaaggtgcct atggacttgg
    14281 agcaactaaa ttagaagtag caactaaagt cgtacttcct gcagcaacat caggtattgt
    14341 agcttcaatc gttctcgcga tttcaagagc aattggagaa acgatgattg tatcattagc
    14401 ggcaggtagt tcgccaacag cttcattaag tttaacaagt tcgattcaaa caatgactgg
    14461 atatattgtt gagatagcga caggtgatgc aacatttgga tcaaatattt attacagtat
    14521 ttatgctgta ggattcacat tatttatctt taccttaatc atgaatttac tttctcaatg
    14581 gatttctaag cgttttaggg aggagtatta atatggaaat gacagataat aatagacaat
    14641 cactcgtcga tcaacaactt gtccaaaaac atttatcatc cagaacggtt aaaaataaag
    14701 tgttcaaact catattttta gcatgtacat tattaggact tgtcgtactt attgcgttgt
    14761 taactcaaac attgattaag ggggtaagtc atttaaattt acagtttttc actaattttt
    14821 cttcttcaac accatctatg gctggcgtta aaggcgcgtt aatcggttca ctttggttaa
    14881 tgttaagtat cattccatta tcaatcatcc taggaatagg tacagctata tacttagaag
    14941 aatatgcgaa aaacaacaaa tttactcagt ttgttaaaat cagtatttcc aatttagctg
    15001 gtgtaccatc agttgtattt gggttattag gttacacttt gttcgttggt ggcgcgggga
    15061 ttgaagcctt gaaaatgggt aacagtatat tggcagcagc gctaacaatg accttactga
    15121 tattaccaat tattattgtt tcaagtcagg aagcaattag agctgtacct aactcagtac
    15181 gcgaagcttc ttacggttta ggtgctaata aatggcaaac gataagacgt gttgtcttac
    15241 cagcagcgtt acctggtatt ttaactggat tcattttgtc tctttcaaga gcactgggag
    15301 aaacagcgcc acttgtgctt atcggtatac cgactatatt attggcaaca cctaggagta
    15361 tattggatca attttcagca ttacctatcc aaatatttac ttgggcgaaa atgcctcaag
    15421 aagaattcca gaatgttgca tcggcaggca ttatcgtttt actagttatc ttaatattaa
    15481 tgaatggcgt tgcgattatt ttacgtaaca aatttagtaa aaaattctaa tttaaacaat
    15541 caatctcatt tatctattaa aaagggagtt ttaaacatgg cgcaaacact tgcacaaact
    15601 aaacaaatat ctcaaagtca tacgtttgat gtctcacaaa gtcatcataa aacaccagat
    15661 gatacaaact cacattctgt tatatattca acacaaaatt tagacttatg gtatggcgaa
    15721 aatcatgcat tgcaaaatat taatttagat atttatgaaa atgaaataac tgccattata
    15781 ggtccatctg gttgtggcaa atcaacatat attaaaactt tgaatcgaat ggttgagtta
    15841 gtacccacag tcaaaacagc tggtaaaata ctatatcgcg atcaagacat atttgatcaa
    15901 aaatattcta aagaacaatt acgtacaaat gtgggcatgg tctttcaaca acctaatcca
    15961 tttccaaaat caatatacga taatattact tacggtccaa agattcacgg tattaaaaat
    16021 aaaaaagttc ttgatgaaat cgttgagaaa tcattacgtg gcgcggcaat ttgggatgaa
    16081 ctaaaggata ggttgcacac aaatgcatat agtttatccg gcgggcaaca acagcgtgtt
    16141 tgtatcgcgc gttgtttagc aattgaacct gaagtcattt taatggatga accgacatca
    16201 gcattagacc caatctcaac attaagagta gaagagttgg ttcaagaact aaaagaaaag
    16261 tatacaatta ttatggttac acataatatg caacaagcag ctcgtgtatc agataaaact
    16321 gcatttttct taaatggtta tgtcaatgaa tatgatgata ctgataaaat tttctctaac
    16381 ccatcaaaca agaaaacaga agattatatt tcaggaaggt ttggttgata tataatggca
    16441 ataattagac aacgatatca ggagcaactt gatgatttaa taaaagaatt acgtcggtta
    16501 ggtgcaaatg tctatgtgag tattgaaaat ggtataaaat cattaagtat tgacgataga
    16561 ggctttgcac gacaaacagt taaaaacgat aaacatatca atcaattaaa ttataatatt
    16621 aatgagcgag ttatcatgtt aattacaaag caacagccca ttgcgagtga tttgcgtatg
    16681 atgatttctt cattaaaaat cgcctccgat ttagaaagaa taggagataa tgcatcgagt
    16741 attgccaata ttcgattgcg tacgaagatt acagatgatt atgtgttaac ccgtttaaag
    16801 acaatgggta aattagctat gttaatgtta aaggacttag atcaagcatt taaaaagaaa
    16861 gataccgtat taataagaga aataattgag cgtgatgaag atatcgatga cttatatagt
    16921 catattatta acgcaacgta tcttattgat aacgatccat ttgtcgctgc acaagctcat
    16981 ttagcagcaa gacatttaga acgtattggt gatcatatta ttaacatcgc tgaaagtgtt
    17041 tatttttatt taacaggtac acattacgaa caataactta aagttattac tataaaatcc
    17101 cttacgataa atatatattt ctattattca taaaccctca aaaaaaaaac caagattctc
    17161 acaattagta atgtgaaaat cttggtttat attgttctac tataaattgt ctcgcatctt
    17221 agttatttgc ttgctcaatt tcatctgtta atttttcaac ttcatcgact aaatcagaaa
    17281 tatattgaat tgtagattta agtggctgtt ctgtagtaat gtccacacct gcaatgtttg
    17341 caagttcgac aggtgataca ctaccacctt ttttcaatgt ttctaaccaa gcatcaacag
    17401 cttgttggcc ttcattttta atcttttgag aaacgacagt tccgattgtt aagccagcag
    17461 aatacgtata cgaatataat cccatatagt aatgaggttg acgcatccat gttaattcag
    17521 caccctcagt catgtctact acatctccaa aaaattgttt ataaacattt agcattattt
    17581 catttaatgt cggcgcattt aaagattcac cttgatctac tttgtgatac acttcacgtt
    17641 gataagcagc ttctaataaa tgggtaacca tattatgata atatgttcta gataaaattg
    17701 agccaataac ccaacgctta aatcttggat tatcacttgt gttaaataaa taattggcca
    17761 tcaacatttc attcattgta gaaggggctt caacaaagta cattgatgct tctgattcaa
    17821 gatatggttg atgtttttga gctgatgtaa aatgacctgc atgacctaat tcatgtgcta
    17881 ggacaaatgc ttcagccatt ttaccagtcc aagaaatgaa cacatacgaa tgtgtaaagt
    17941 atggacttgc acaaaatgcg cctgtatctt tacctttatt ttgtgcaaaa tcaatccatc
    18001 gctgatcgta tgcttcacgt aacatgtttg tatagtcatc acctaaaaca cttaacgcac
    18061 cgaaaatata attttttgag tcttcaattg aaatctctgg ttcataatca gggtctacag
    18121 aaattttcaa gtcttcaaaa cgcatgttat ctaattcgtg aatacgttgt aaaagtttag
    18181 catatttctg cataactggt gccaagtcac gcataatcat atcgatttga cggtcaaaca
    18241 tatcacgcgt tacttcttga ctatgtagta aataatcgat gaccgattca aatccacgta
    18301 aatcagcttc aattttttct tgttgtactt gcatattata tgtagccgcg gtagtatgct
    18361 gatattttcg aatcccatcg ctaaacgatt tgaaactttt acgtctaaac tcaggatctt
    18421 tattatcttc ataatcattt tcaaacgtag catagtcgac agggtacgtt gtaccatcat
    18481 gttcaaatga atcgaatgta atatctagca tttttgtcgt gccgtataaa tcgtaaggac
    18541 tgtttagcgt aggtgataaa gttgctaata cttgttctac cgacgcagat aattggaatg
    18601 gcttttgttt tattaactgt ttaatatagt gttgatatgg acatgattct tctaattgtt
    18661 gaagtatttc ttctggtaat tcaagtattt ctgactctac aaatgataat tggctaacaa
    18721 ttttaccata tgtagtagat aatttagcgc tcaatacttg cgcctcaata ttactagtat
    18781 ctacacttaa acgtagttct gcataattac ttaagcgatc taaggcaatt aaaatatttt
    18841 ctaattcagc taaagcagta ttaatttgtt cgattgaatt taatgttgtt gcatatgtat
    18901 gatgaaattg atttgcttgt tgtactagag cgtcaatact ttcataatat tgttgatcat
    18961 ctttaaataa gtctactaaa tcccatgttt ctgaaacagg aacatcttct cttaaaagta
    19021 aaccttgaga catataatca ctcctttggt ataatacatt cattatacat aattttgtct
    19081 cctaaacgat aatgttacga tttccatgtc gcaaggaaca atgatttttc taatagtaga
    19141 tagaatattg cttgaaactg actgctattg tttaagtttt taaaaattta taataaaatg
    19201 tatacaagga aagtaatgaa ataaaaggag aatgaatatg
//
LOCUS       DAHHOJ010000048        19024 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.048, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000048 DAHHOJ010000000
VERSION     DAHHOJ010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 19024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.048"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            137..1327
                     /locus_tag="NBH32_001432"
     CDS             137..1327
                     /locus_tag="NBH32_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HCD1515035.1"
                     /translation="MTKSEKIIELTNHYGAHNYLPLPIVISEAEGVWVKDPEGNKYMD
                     MLSAYSAVNQGHRHPKIIQALKDQADKVTLVSRAFHSDNLGEWYEKICKLAGKDKALP
                     MNTGAEAVETALKAARRWAYDVKGIEPNKAEIIAFNGNFHGRTMAPVSLSSEAEYQRG
                     YGPLLDGFRKVDFGDVDALKAAINENTAAVLVEPIQGEAGINIPPEGYLKAIRELCDE
                     HNVLFIADEIQAGLGRSGKLFATDWDNVKPDVYILGKALGGGVFPISVVLADKEVLDV
                     FTPGSHGSTFGGNPLACAASIAALDVIVDEDLPGRSLELGDYFKEQLKQIDHPSIKEV
                     RGRGLFIGVELNESARPYCEALKEEGLLCKETHDTVIRFAPPLIITKEELDLALEKIR
                     HVFQ"
     gene            1436..2680
                     /locus_tag="NBH32_001433"
     CDS             1436..2680
                     /locus_tag="NBH32_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HCD1515036.1"
                     /translation="MTENNNLVTSTQGIIKEALHKLGFDEGMYDLIKEPLRMLQVRIP
                     VRMDDGTVKTFTGYRAQHNDAVGPTKGGVRFHPDVDEEEVKALSMWMTLKCGIVNLPY
                     GGGKGGIVCDPRQMSIHEVERLSRGYVRAISQFVGPNKDIPAPDVFTNSQIMAWMMDE
                     YSALDKFNSPGFITGKPIVLGGSHGRDRSTALGVVIAIEQAAKRRNMQIEGAKVVIQG
                     FGNAGSFLAKFLYDLGAKIVGISDAYGALHDPNGLDIDYLLDRRDSFGTVTNLFEETI
                     SNKELFELDCDILVPAAISNQITEDNAHDIKASIVVEAANGPTTPEATRILTERGILL
                     VPDVLASAGGVTVSYFEWVQNNQGYYWSEEEVNEKLREKLEAAFDTIYELSQNRKIDM
                     RLAAYIIGIKRTAEAARYRGWA"
     gene            complement(2962..3891)
                     /locus_tag="NBH32_001434"
     CDS             complement(2962..3891)
                     /locus_tag="NBH32_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCD1515037.1"
                     /translation="MTNSSKSFTKFMAASAVFTMGFLSVPTAGAEQTNQIANKPQAIQ
                     WHTNLTNERFTTIAHRGASGYAPEHTFQAYDKSHNELKASYIEIDLQRTKDGHLVAMH
                     DETVNRTTNGHGKVEDYTLDELKQLDAGSWFNKKYPKYARASYKNAKVPTLDEILERY
                     GPNANYYIETKSPDVYPGMEEQLLASLKKHHLLNNNKLKNGHVMIQSFSDESLKKIHR
                     QNKHVPLVKLVDKGELQQFNDQRLKEIRSYAIGLGPDYTDLTEQNTHHLKDLGFIVHP
                     YTVNEKADMLRLNKYGVDGVFTNFADKYKEVIK"
     gene            complement(4133..5512)
                     /gene="argH"
                     /locus_tag="NBH32_001435"
     CDS             complement(4133..5512)
                     /gene="argH"
                     /locus_tag="NBH32_001435"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCD1515038.1"
                     /translation="MSNKAWGGRFEVQPEEWVDDFNASITFDQTLIDQDIEGSIAHAT
                     MLANQGIISQQDSEQIIQGLKSIQHDYHQDQIQFSASLEDIHLNIEHELIKRIGDAGG
                     KLHTGRSRNDQVATDMHLYTKKQVQDIIALIKSLQSVIVDIASNNVDTIMPGYTHLQR
                     AQPISFAHNIMTYFWMLQRDQQRFEDSLKRIDINPLGAAALSGTTYPIDRHETTALLN
                     FGSLYENSLDAVSDRDYIIETLHNISLTMVHLSRFAEEIIFWSTDEAKFITLSDAFST
                     GSSIMPQKKNPDMAELIRGKVGRTTGHLMSMLMTLKGLPLAYNKDMQEDKEGLFDAVH
                     TIKGSLRIFEGMIQTMTINKEQLNQTVKEDFSNATELADYLVTKNIPFRTAHEIVGKI
                     VLECIQQGHYLLDVPLATYQQHHSSIDADIYDYLQPENCLKRRQSYGSTGQSSVKQQL
                     DVAKQLLSQ"
     gene            complement(5502..6707)
                     /locus_tag="NBH32_001436"
     CDS             complement(5502..6707)
                     /locus_tag="NBH32_001436"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCD1515039.1"
                     /translation="MKEKIVLAYSGGLDTSVAVQWLIDKGYDVVACCLDVGEGKDLDI
                     VYKKALDMGAVECHIIDATKEFSDEYVSYAIKGNLMYENAYPLVSALSRPLIAKKLVE
                     IAEKTNSVGIAHGCTGKGNDQVRFEVAIKALNPSLKAFAPVREWAWSREEEIDYAIKH
                     NIPVSINHDSPYSIDQNLWGRANECGILEDPYAAPPEDAFDLTNALEETPDTADEIIL
                     TFDKGIPVQIDGKTYELDDLILTLNALAGKHGIGRIDHVENRLVGIKSREIYEAPAAE
                     VILKAHKALETITLTKDVAHFKPIIEKQFAEQLYNGLWFSPLTDSLKLFIDSTQQYVS
                     GDVRIKLFKGNTIVNGRKSPYTLYDEKLATYTKEDAFNQDAAVGFIDIYGLPTQVNAM
                     LHGGYSNEQ"
     gene            7058..8389
                     /locus_tag="NBH32_001437"
     CDS             7058..8389
                     /locus_tag="NBH32_001437"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCD1515040.1"
                     /translation="MTHIQLDFSKTLEFFGEHELKQQQEIVKSIHKTIHEGTGAGSDF
                     LGWVDLPVDYDKEEFSRIVEASKRIKENSDVLVVIGIGGSYLGARAAIEMLTSSFRNS
                     NEYPEIVFVGNHLSSTYTKELVDYLADKDFSVNVISKSGTTTEPAVAFRLFKQLVEER
                     YGKEEAQKRIFATTDKEKGALKQLATNEGYETFIVPDDVGGRYSFLTAVGLLPIATAG
                     INIEAMMIGAAKAREELSSDKLEENIAYQYATIRNILYAKGYTTEMLINYEPSMQYFN
                     EWWKQLFGESEGKDFKGIYPSSANYTTDLHSLGQYVQEGRRFLFETVVKVNHPKYDIT
                     IEKDSDDLDGLNYLAGKTIDEVNTKAFEGTLLAHTDGGVPNMVVNIPQLDEETFGYVV
                     YFFELACAMSGYQLGVNPFNQPGVEAYKQNMFALLGKPGFEDLKKELEERL"
     gene            8714..9289
                     /locus_tag="NBH32_001438"
     CDS             8714..9289
                     /locus_tag="NBH32_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCD1515041.1"
                     /translation="MSFHQVEEWFEIFRQFGYLPGFILLYIRAIIPVFPLALYILINI
                     QAYGPILGILISWLGLISGTFTVYLICKRWVNTERMQRIKQRTAVQRLISFIDRQGLI
                     PLFILLCFPFTPNTLINFVASLSHIRPKYYFIVLASSKLVSTIILGYLGKEITTILTQ
                     PLRGILMLVVLVVFWIVGKKLEQHFMGSKKE"
     gene            9294..9818
                     /gene="lepB"
                     /locus_tag="NBH32_001439"
     CDS             9294..9818
                     /gene="lepB"
                     /locus_tag="NBH32_001439"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1515042.1"
                     /translation="MKKVVKYLISLILAIIIVLFVQTFVIVGHVIPNNDMSPTLNKGD
                     RVIVNKIKVTFNQLNNGDIITYRRGNEIYTSRIIAKPGQSMAFRQGQLYRDDRPVDAS
                     YAKNRKIKDFSLRNFKELDGDIIPPNNFVVLNDYDNNQHDSRQFGLIDKKDIIGNISL
                     RYYPFSKWTIQFKS"
     gene            9834..10409
                     /gene="lepB"
                     /locus_tag="NBH32_001440"
     CDS             9834..10409
                     /gene="lepB"
                     /locus_tag="NBH32_001440"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCD1515043.1"
                     /translation="MKKELLEWIISIAVAFVILFIVGKFIVTPYMIKGESMDPTLKDG
                     ERVAVNIIGYKTGGLEKGNVVVFHANKNDDYVKRVIGVPGDKVEYKNDTLYVNGKKQD
                     EPYLNYNLKHKQGDYITGTFQVKDLPNANPKSNVIPKGKYLVLGDNREVSKDSRAFGL
                     IDEDQIVGKVSFRFWPFSEFKHNFNPENTKN"
     gene            10569..14045
                     /gene="addB"
                     /locus_tag="NBH32_001441"
     CDS             10569..14045
                     /gene="addB"
                     /locus_tag="NBH32_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddB"
                     /protein_id="HCD1515044.1"
                     /translation="MTLHAYLGRAGTGKSTKMLTEIKQKMKQDPLGDPIILIAPTQST
                     FQLEQAFVNDPELNGSLRTEVLHFERLSHRIFQEVGSYSEQKLSKAATEMMIYNIVQE
                     QQKYLKLYQSQAKYYGFSEKLTEQIQDFKKYAVTPEHLEHFIADKNMQTRTKNKLEDI
                     ALIYREFEQRIQNEFITGEDSLQYFIDCMPKSEWLKRADIYIDGFHNFSTIEYLIIKG
                     LIKYAKSVTIILTTDGNHDQFSLFRKPSEVLRHIEEIANELNISIERQYFKQLYRFNN
                     QDLKHLEQEFDALQINRVACQGHINILESATMREEINEIARRIIVDIRDKQLRYQDIA
                     ILYRDESYAYLFDSILPLYNIPYNIDTKRSMTHHPVMEMIRSLIEVIQSNWQVNPMLR
                     LLKTDVLTASYLKSAYLVDLLENFVLERGIYGKRWLDDELFNVEHFSKMGRKAHKLTE
                     DERNTFKQVVKLKKDVIDKILHFEKQMSQAETVKDFATAFYESMEYFELPNQLMTERD
                     ELDLNGNHEKAEEIDQIWNGLIQILDDLVLVFGDEPMSMERFLEVFDIGLEQLEFVMI
                     PQTLDQVSIGTMDLAKVDNKQHVYLVGMNDGTMPQPVTASSLITDEEKKYFEQQANVE
                     LSPTSDILQMDEAFVCYVAMTRAKGDVTFSYSLMGSSGDDKEISPFLNQIQSLFNQLE
                     ITNIPQYHEVNPLSLMQHAKQTKITLFEALRAWLDDEIVADSWLDAYQVIRDSDHLNQ
                     GLDYLMSALTFDNETVKLGETLSKDLYGKEINASVSRFEGYQQCPFKHYASHGLKLNE
                     RTKYELQNFDLGDIFHSVLKYISERINGDFKQLDLKKIRQLTNEALEQILPKVQFNLL
                     NSSAYYRYLSRRIGAIVETTLSALKYQGTYSKFMPKHFETSFRRKPRTNDELIAQTLT
                     TTQGIPINIRGQIDRIDTYTKNDTSFVNIIDYKSSEGSATLDLTKVYYGMQMQMMTYM
                     DIVLQNKQRLGLTDIVKPGGLLYFHVHEPRIKFKSWADIDEDKLEQDLIKKFKLSGLV
                     NADQTVIDALDIRLEPKFTSDIVPVGLNKDGSLSKRGSQVADEATIYKFIQHNKENFI
                     ETASNIMDGHTEVAPLKYKQKLPCAFCSYQSVCHVDGMIDSKRYRTVDETINPIEAIQ
                     NININDEFGGEQ"
     gene            14046..17699
                     /gene="addA"
                     /locus_tag="NBH32_001442"
     CDS             14046..17699
                     /gene="addA"
                     /locus_tag="NBH32_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HCD1515045.1"
                     /translation="MTIPEKPQGVIWTDAQWQSIYATGQDVLVAAAAGSGKTAVLVER
                     IIQKILRDGIDVDRLLVVTFTNLSAREMKHRVDQRIQEASIADPANAHLKNQRIKIHQ
                     AQISTLHSFCLKLIQQHYDVLNIDPNFRTSSEAENILLLEQTIDEVIEQHYDILDPAF
                     IELTEQLSSDRSDDQFRMIIKQLYFFSVANPNPTNWLDQLVTPYEEEAQQAQLIQLLT
                     DLSKVFITAAYDALNKANDLFSMMDGVDKHLAVIEDERRLMGRVLEGGFIDIPYLTGH
                     EFGARLPNVTAKIKEANEMMVDALEDAKLQYKKYKSLIDKVKSDYFSREADDLKADMQ
                     QLAPRVKYLARIVKDVMSEFNRKKRSKNILDFSDYEHFALQILTNEDGSPSEIAESYR
                     QHFQEILVDEYQDTNRVQEKILSCIKTGAEHNGNLFMVGDVKQSIYKFRQADPSLFIE
                     KYQRFTINGDGTGRRIDLSQNFRSRKEVLSTTNYIFKHMMDEQVGEVNYDESAQLYYG
                     APYDESDHPVNLKVLVEADQEHSDLTGSEQEAHFIVEQVKDILEHQKVYDMKTGSYRS
                     ATYKDIVILERSFGQARNLQQAFKNEDIPFHVNSREGYFEQTEVRLVLSFLRAIDNPL
                     QDIYLVGLMRSVIYQFKEDELAQIRILSPNDDYFYQSIVNYINDEVADAILVDKLKMF
                     LSDIQSYQQYSKDHPVYQLIDKFYNDHYVIQYFSGLIGGRGRRANLYGLFNKAIEFEN
                     SSFRGLYQFIRFIDELIERGKDFGEENVVGPNDNVVRMMTIHSSKGLEFPFVIYSGLS
                     KDFNKRDLKQPVILNQQFGLGMDYFDVDKEMAFPSLASVAYKAVAEKELVSEEMRLVY
                     VALTRAKEQLYLIGRVKNDKSLLELEQLSISGEHIAVNERLTSPNPFHLIYSILSKHQ
                     SASIPDDLKFEKDIAQVEDSSRPNVNISIIYFEDVSTETILDNNEYRSVNQLETMQNG
                     NEDVKAQIKHQLDYQYPYVNDTKKPSKQSVSELKRQYETEESGTSYERVRQYRIGFST
                     YERPKFLSEQGKRKANEIGTLMHTVMQHLPFKKERISEVELHQYIDGLIDKHIIEADA
                     KKDIRMDEIMTFINSELYSIIAEAEQVYRELPFVVNQALVDQLPQGDEDVSIIQGMID
                     LIFVKDGVHYFVDYKTDAFNRRRGMTDEEIGTQLKNKYKIQMKYYQNTLQTILNKEVK
                     GYLYFFKFGTLQL"
     gene            17863..18765
                     /locus_tag="NBH32_001443"
     CDS             17863..18765
                     /locus_tag="NBH32_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCD1515046.1"
                     /translation="MKFLSFKYNDKTSYGVKVKREDAVWDLTQVFADFAEGDFHPKTL
                     LAGLQQNHTLDFQEQVRKAVVAAEDSGKAEDYKISFNDIEFLPPVTPPNNVIAFGRNY
                     KDHANELNHEVERLYVFTKAASSLTGDNATIPNHKDITDQLDYEGELGIVIGKSGEKI
                     PKALALDYVYGYTIINDITDRKAQSEQDQAFLSKSLTGGCPMGPYIVTKDELPLPENV
                     NIVTKVNNEIRQDGNTGEMILKIDELIEEISKYVALHPGDIIATGTPAGVGAGMQPPK
                     FLQPGDEVKVTIDNIGTLTTYIAK"
BASE COUNT         6648 a         2781 c         3439 g         6156 t
ORIGIN      
        1 aaaatagaca aaattaatga aacttagtga ttatttcatc ttttttggtg gaaaagtatc
       61 tgctttgaaa actaatgtaa ttatttggat aatgtttcat gtataatagt tataaatgat
      121 gaaaagggga gatatgatga ctaaatctga aaaaattatt gagttaacaa atcattacgg
      181 agcacataat tatttaccat tgccaattgt catttcagaa gctgaagggg tatgggttaa
      241 agatcctgaa ggcaataaat atatggatat gttatctgca tattccgctg ttaaccaagg
      301 tcatagacat ccgaaaatta ttcaagcatt aaaagatcaa gctgataaag tgactctagt
      361 ttcacgtgct tttcatagtg ataacttagg tgaatggtac gaaaaaattt gtaaactggc
      421 aggtaaagat aaagctttac caatgaatac aggtgctgaa gcagtagaaa cagctttgaa
      481 agcagcacga cgctgggcat acgatgttaa aggaattgag ccaaataaag cagaaatcat
      541 tgcatttaat ggtaacttcc atggtcgaac aatggcgcca gtttcattat cttcagaagc
      601 agaataccaa cgtggttatg gtccgttatt agatggattt agaaaagttg attttggaga
      661 tgtagatgca ttgaaagctg caattaatga aaatactgca gcagttttag tagaaccaat
      721 tcaaggtgaa gcgggtataa atataccgcc agaaggatat ttgaaagcaa ttagagaatt
      781 atgtgatgaa cataatgtct tatttattgc tgacgaaatc caagcaggat taggtcgttc
      841 gggtaaatta tttgctacgg attgggataa tgtaaaacct gatgtctata ttttaggtaa
      901 agcactaggt ggtggtgtct tcccaatttc tgttgtatta gcagataaag aagtattaga
      961 tgtctttaca cctggctcac atggttcaac atttggtggt aatccacttg cttgtgctgc
     1021 atcaattgct gcattagatg ttatcgttga tgaggattta ccagggcgct ctttagaatt
     1081 aggagattat tttaaagaac aattaaagca aattgatcat ccatcaatta aagaagtccg
     1141 tggacgtggt ttgtttatag gtgtggaact taatgaaagt gctagaccat attgtgaagc
     1201 tttgaaagaa gaaggcttat tatgtaaaga aacgcatgat actgtcattc gttttgcacc
     1261 accattaatt attactaaag aagaattgga ccttgcactt gaaaaaataa gacacgtatt
     1321 tcaataatgt caaaaagttg taacaatcag tttgtaaggg ctttaaaaag gaatacgtta
     1381 tgttacaata tgtttgaaag cgaaatcatt aggtaaagaa gaggcgaaaa ggatcatgac
     1441 tgagaacaat aatttagtaa cttctactca aggaattatt aaagaagcat tgcataaatt
     1501 gggatttgac gaaggaatgt acgatttaat taaagaacct ttaagaatgt tacaagtgcg
     1561 tatccctgta cgaatggatg atggtactgt taaaacattc acaggttacc gtgcgcaaca
     1621 taatgatgct gttggaccaa caaaaggggg cgtgcgtttc cacccagatg ttgatgaaga
     1681 agaagtaaaa gcattatcaa tgtggatgac tttgaaatgt ggcattgtaa acttaccata
     1741 cggtggtggt aagggtggta tcgtttgtga tccacgtcaa atgagcattc atgaagttga
     1801 acgtttatca cgcggatatg taagagcaat ttcacaattc gtaggtccga acaaagatat
     1861 tccagcacca gatgtattta caaactcaca aattatggct tggatgatgg atgaatatag
     1921 tgcattagat aaatttaatt caccaggttt catcacaggt aaaccaattg tattgggtgg
     1981 ttctcatgga cgcgacagat caactgcact aggtgtagtt attgcaattg aacaagctgc
     2041 aaaacgtcgt aatatgcaaa ttgaaggtgc caaggttgtt attcaaggtt tcggtaatgc
     2101 cggaagtttc ttagctaaat tcttatatga tttaggtgca aaaattgtag gtatctctga
     2161 tgcttacggt gcattacacg atccaaatgg cttagatata gattatttat tagaccgtcg
     2221 tgatagtttt ggtacggtaa caaatttatt tgaagaaaca atctcaaata aagaattgtt
     2281 tgaattagat tgtgacattt tagtaccagc ggctatttca aaccaaatta cagaagacaa
     2341 tgcacatgat attaaagcta gcatcgttgt tgaagctgct aatggaccta caacaccaga
     2401 agcaacacgt attttaactg aacgtggtat attattagtt ccagacgtat tagcaagtgc
     2461 tggtggtgta acggtttctt acttcgaatg ggtacaaaat aatcaaggtt attattggtc
     2521 tgaagaagaa gttaatgaaa agctacgtga aaaattagaa gcggcatttg atacgattta
     2581 cgaattgtct caaaatcgaa aaatagatat gagacttgca gcatatatca taggtattaa
     2641 acgtacagca gaagcagcta gatatcgtgg ttgggcataa ttaattatca tatgtgattt
     2701 aacgagcttg ggacagaaaa caaagcccta agctcgttaa ttttatttta gcagtagttg
     2761 actgaataaa aatacacttg taacaagctt atttcaaaaa tagccggggc cccgaacaca
     2821 gagaatttct aattaaacgg tcaactatat atagttgatt aaaaagacag cagtaagata
     2881 ttttctattc ggaaatatct tactgctgtc ttttttagta atagctatat ttttgtattt
     2941 atgttttcta gtttaacaat actacttaat gacttcttta tatttgtcag cgaaatttgt
     3001 aaagacacca tcaacgccgt atttatttaa tcgtaacata tcagcttttt catttactgt
     3061 ataaggatgt actataaaac ctaagtcttt taaatgatgg gtattttgtt cagttaaatc
     3121 tgtataatca ggacctaatc caatcgcata agagcgtatc tcttttaagc gttggtcgtt
     3181 aaattgttgt agttcacctt tatcaactaa ttttactaat ggcacatgtt tattttgacg
     3241 atgaattttc tttaaacttt cgtcagaaaa tgattgaatc attacatgtc cattttttaa
     3301 tttattgtta tttaaaaggt gatgcttttt caatgaagct aataattgtt cttccattcc
     3361 tgggtataca tcaggtgact ttgtttcaat ataatagttt gcattcgggc cataacgttc
     3421 taaaatttca tctaaagtgg gtactttagc atttttataa cttgctcttg cgtattttgg
     3481 atatttttta ttaaaccaac ttcctgcatc taactgtttt aattcatcaa gggtataatc
     3541 ctcaacttta ccgtgtccat ttgttgtacg gttaacagtt tcatcatgca tagcaactaa
     3601 atggccatct ttggtacgtt gtaaatcaat ttcgatataa gatgctttta actcattatg
     3661 actcttatca tatgcttgaa acgtatgctc gggtgcatag ccacttgcgc cacgatgtgc
     3721 gatagtagtg aatcgctcat tcgttaaatt tgtatgccat tgaatagcct gaggtttatt
     3781 tgcaatttga tttgtttgtt cagcgccagc agtaggtact gataaaaatc ccatagtaaa
     3841 aacagcagaa gcagccataa atttagtgaa gcttttcgaa gagttagtca tagtctttat
     3901 cctttcatct ctttatttgt agtctaacaa ttttaaaata ctacaaaaac ctgcttgtga
     3961 aaacgcttta cacatttctt ttacaattta taacatttct gtaatataaa tttaaaagaa
     4021 ttatcaaaca ttttaggaat cttaaaaatg tgattttcaa caagacgaag agagcattaa
     4081 aatacccaca atgtaatttt aatagacatt gtgggtaggt agattaacgt atttattgtg
     4141 atagtaattg tttagcaaca tcaagttgtt gtttgaccga tgattgacct gttgaaccgt
     4201 aactttgacg tcgttttaaa caattttcag gctgcaaata atcgtaaata tcggcatcaa
     4261 tactagaatg atgttgttga tatgttgcta aaggaacatc taataaataa tgaccttgtt
     4321 gtatacattc taagacaatt tttcctacaa tttcatgtgc agttctaaat ggaatatttt
     4381 tagttactaa ataatctgct agttccgttg catttgaaaa atcttcttta acagtttgat
     4441 tgagttgttc tttattaatt gtcatcgttt gaatcatacc ttcgaaaata cgtaaagaac
     4501 ctttaattgt atggacagca tcgaataaac cttctttatc ttcctgcata tctttgttat
     4561 atgctagagg taatcctttt aaagtcataa gcatgctcat taaatgacca gtcgttcgac
     4621 caactttacc tctaattaat tctgccatat caggattttt cttttgtggc ataatagatg
     4681 agccagttga aaatgcatct gataatgtaa tgaatttagc ttcgtctgtg gaccagaaaa
     4741 taatttcctc tgcaaagcgt gataagtgaa ccatcgttaa agaaatatta tgcaatgttt
     4801 caataatata gtctctgtca ctaacagcat ctaggctatt ctcatagaga ctgccaaagt
     4861 tcaacaatgc tgttgtctcg tgtctatcga tagggtatgt ggtaccactt aaggctgctg
     4921 cacctaaagg attaatatcg attcgtttta aactatcttc aaatcgttgt tggtctcgtt
     4981 gtaacatcca aaaataagtc ataatattat gtgcaaatga aattggctgt gcacgctgta
     5041 aatgagtata accaggcata attgtatcaa cattattgga agcgatgtct acaattacac
     5101 tttgtaacga cttaattaat gcgatgatat cttgcacttg tttcttagtg tacaagtgca
     5161 tatctgttgc aacttgatcg tttctactgc gtccagtatg caacttacca ccagcatcac
     5221 caatacgttt aattaattca tgttcaatat ttaaatgaat atcttctaat gatgcactaa
     5281 attgaatttg atcttgatga taatcatgtt gaatagattt tagtccttgt ataatttgtt
     5341 cgctgtcttg ttgactaata atgccttgat tcgcaagcat agttgcatgt gcaatgctgc
     5401 cttcgatatc ttgatctatg agcgtttgat caaaagtaat ggatgcgtta aagtcgtcaa
     5461 cccactcttc aggttgtact tcaaatctac cgccccaagc tttattgctc attgctatag
     5521 cctccatgca acatcgcatt tacttgagta ggtaaaccat agatatcgat aaagccaaca
     5581 gcagcgtctt gattaaatgc atcttctttt gtataagttg ctaatttttc atcatataat
     5641 gtgtaaggtg attttctacc atttacgatg gtattacctt tgaataattt aattctgaca
     5701 tcaccactta cgtattgctg agtactatca ataaataatt ttaagctatc agttaaaggt
     5761 gagaaccaaa gtccattgta tagttgttca gcaaattgct tctcgatgat tggtttaaag
     5821 tgtgcgacat ctttcgttaa cgtaatcgtt tctaatgctt tatgcgcttt taaaataact
     5881 tctgcagcag gtgcttcata aatttctctt gatttgatac ctacaagtct attttctaca
     5941 tggtcaattc ttccgatacc atgcttacca gctaatgcat tcaacgttaa aattaaatcg
     6001 tctaattcat atgttttgcc atcaatttga actgggatgc ctttatcaaa cgttaaaatg
     6061 atttcatcag cagtatctgg tgtttcttct aaagcatttg ttagatcgaa cgcatcctct
     6121 ggtggcgcag cataaggatc ttctaaaata ccacattcat tcgctctgcc ccatagattt
     6181 tgatcgatag aataaggtga atcatggttg attgatacag ggatattatg tttaattgca
     6241 taatcgattt cttcttcacg actccatgcc cactcacgta caggtgcgaa tgctttcaat
     6301 gatgggttta atgctttaat ggcaacttcg aaacgtactt ggtcattccc tttaccagta
     6361 caaccatgtg caatacctac tgaatttgtt ttctcagcaa tctctactaa ttttttagcg
     6421 attaatggtc ttgataaagc tgaaactaat ggatatgcat tttcatacat taaatttcct
     6481 ttgattgcat aacttacata ctcatcacta aattcttttg ttgcatcaat aatatgacat
     6541 tcaactgctc ccatatctaa agctttttta taaacgatgt ctaaatcttt accttcacca
     6601 acatctaggc aacaagctac aacatcgtat cctttgtcga taagccattg aacggccaca
     6661 cttgtatcta gtcctcctga atatgctaaa acaattttct ctttcataaa attcacctca
     6721 ttgtagttat ctgaatattt cgtatgaaca catgatagca aacttttaat gaaaataaaa
     6781 ccattatttt acatatttat tcataaaatg cgatagagat agttagagtt gtaagtataa
     6841 atgcttattt atcaatgttt aaaagtattt taaaaaacat gcaaattgta taaaaaatgt
     6901 ttgagtgttt gacgccagtt tgaaatgttt gttgaaaaat aggtgtgaaa cgtcacataa
     6961 atatcaattt tattgattaa gtacaaactt gtgaattgaa tcgcaaacgt aaacttagta
     7021 aaattaagat attaaatgaa acaggaggct tttatagatg actcatattc aattagattt
     7081 tagtaaaacg ttagaatttt tcggtgaaca cgaattaaaa caacaacaag aaattgttaa
     7141 atcaattcac aaaacaattc atgaaggtac tggtgcaggt agtgacttct taggctgggt
     7201 tgatttacca gttgattacg acaaagaaga attttcaaga attgttgaag catcaaaacg
     7261 cattaaagaa aattctgatg ttttagtagt catcggtatt ggtggttctt acttaggtgc
     7321 acgtgcagca atcgaaatgt taacgtcatc atttagaaac agcaatgaat accctgaaat
     7381 tgtatttgtt ggtaatcact tgtcatcaac atatacgaaa gagttagttg attatttagc
     7441 agacaaagat ttctctgtaa acgttatttc taaatctggt acaactacag aaccagcagt
     7501 tgcatttaga ttgttcaaac aattagttga agaaagatac ggtaaagaag aagcacaaaa
     7561 acgtatattt gcaacaacgg ataaagaaaa aggtgcttta aaacagttgg ctacaaacga
     7621 aggttatgaa acgtttatcg tacctgatga tgtaggtgga agatattctt ttttaacagc
     7681 agtaggatta ttaccaattg caacagctgg aattaacatc gaagctatga tgattggtgc
     7741 tgcaaaagca cgtgaagaat tatcttcaga taaattagaa gaaaacattg catatcaata
     7801 tgcgacaatt cgaaacattt tatatgcaaa aggttataca acagaaatgt tgattaacta
     7861 tgaaccatct atgcaatact ttaatgaatg gtggaaacaa ttatttggtg aatcagaagg
     7921 taaagacttc aaaggtatct atccttcaag tgccaactac acaactgatt tacattcttt
     7981 aggtcaatat gtacaagaag gacgtcgttt cttattcgaa acagttgtaa aagtaaatca
     8041 tcctaaatat gatattacta ttgaaaaaga tagtgatgat ctagacggat taaattattt
     8101 agctggtaaa acaatcgacg aagttaacac aaaagcattc gaaggtacat tattagcaca
     8161 tactgatggt ggtgttccta acatggtagt gaacattcca caattagatg aagaaacttt
     8221 cggttatgtc gtatacttct tcgaacttgc ttgtgcaatg agtggatacc aattaggcgt
     8281 aaatccattt aaccaacctg gtgtagaagc atataaacaa aacatgttcg cattattagg
     8341 taaacctggt tttgaagact tgaaaaaaga attagaagaa cgtttataaa atacattact
     8401 tcaaagatta gtgaagtttg aaaagataga actagacgtt aactatttaa agcatatttt
     8461 cgaggttgtc attacaaatg taaaaatgta atgacaacct cgtttttatt tatatgcaag
     8521 aactaggtta ctagctaatg tgacaagatg ttaagagaaa attaaagaaa aaataacatc
     8581 tgtcatacaa taatattgtt atactactag agactgattt attagcatga ttacatgtta
     8641 atgtttcttt acttagtaat taactttata atgtaagaat aattatcttc agccaaagaa
     8701 agggattgat gatttgtcgt ttcatcaagt agaagaatgg tttgagatat ttcgacagtt
     8761 tggttattta cctggattta tattgttata tattagagcg ataattccag tatttccttt
     8821 agcactctat attttaatta acattcaagc ttatggacct attttaggta tattgattag
     8881 ttggcttgga ttaatttctg gaacatttac agtctatttg atctgtaaac gatgggtgaa
     8941 cactgagagg atgcagcgaa ttaaacaacg tactgctgtt caacgcttga ttagttttat
     9001 tgatcgccaa ggattaatcc cattgtttat tttactttgt tttcctttta cgccaaatac
     9061 attaataaat tttgtagcga gtctatctca tattagacct aaatattatt tcattgtttt
     9121 ggcatcgtca aagttagttt caacaattat tttaggttat ttaggaaaag aaattactac
     9181 aattttaacg caacctttaa gagggatatt aatgttagtt gtgttggttg tattttggat
     9241 tgttggaaaa aagttagaac agcattttat ggggtcgaaa aaggagtgac atcgtgaaaa
     9301 aagttgtaaa atatttgatt tcattgatac ttgctattat cattgtactg ttcgtacaaa
     9361 cttttgtaat agttggtcat gtcattccga ataatgatat gtcaccaacc cttaacaaag
     9421 gggatcgtgt tattgtaaat aaaattaaag ttacatttaa tcaattgaat aatggtgata
     9481 tcattacata taggcgtggt aacgagatat atactagtcg gattattgcc aaacctggtc
     9541 aatcaatggc gtttcgtcag ggacaattat accgtgatga ccgaccggtt gacgcatctt
     9601 atgccaagaa cagaaaaatt aaagatttta gtttgcgcaa ttttaaagaa ttagatggag
     9661 atattatacc gcctaacaat tttgttgtgc taaatgatta tgataacaat cagcatgatt
     9721 ctagacaatt tggtttaatt gataaaaagg atattattgg taatataagt ttgagatatt
     9781 atcctttttc aaaatggacg attcagttca aatcttaaaa agaggtgtca aaattgaaaa
     9841 aagaattatt ggaatggatt atttcaattg cagtcgcttt tgtcatttta tttatagtag
     9901 gtaaatttat tgttacgcca tatatgatta aaggtgaatc aatggatcca actttgaaag
     9961 atggtgagcg agtagctgta aacattattg gatataaaac aggtggtttg gaaaaaggta
    10021 atgtagttgt cttccatgca aacaaaaatg atgactatgt taaacgtgtt atcggtgttc
    10081 ctggtgataa agtagaatat aaaaatgata cattatatgt caatggtaaa aaacaagatg
    10141 aaccatattt aaactacaat ttaaaacata aacaaggtga ttatattact gggactttcc
    10201 aagttaaaga tttaccgaat gcgaatccta aatcaaatgt cattccaaaa ggtaaatatt
    10261 tagtgcttgg agataatcgt gaagtaagta aagatagccg tgcgtttggc ctcattgatg
    10321 aagaccaaat tgttggcaaa gtttcattta gattctggcc atttagtgaa tttaaacata
    10381 atttcaatcc tgaaaacact aaaaattaat atgaaacaaa taaaacatcg tttgtcggtt
    10441 ttaatactga taaacgatgt tttattttgt tagtaccaca ataaaagcta agttcgaaat
    10501 gaacttataa taaatcaatc acaatcactt tgtgttaaaa tatgtgtcaa aggaagtgag
    10561 ggtttgtcat gacattacat gcttatttag gaagagcagg aacaggtaag tctacgaaaa
    10621 tgttaaccga aataaaacaa aaaatgaaac aggatccgct tggagatcca atcattttaa
    10681 ttgcgccaac tcaaagtaca tttcaattag aacaagcctt tgtcaatgat ccggaattaa
    10741 atggtagttt aagaacagaa gtgttgcatt ttgaacgatt aagtcatcgt attttccaag
    10801 aagttggtag ttatagcgaa caaaagttat ctaaagctgc aacggaaatg atgatttata
    10861 acattgttca agaacaacaa aagtatttaa aactttatca atcacaagca aaatattatg
    10921 gttttagtga aaaattaaca gaacaaattc aagattttaa aaaatatgca gtaacgcctg
    10981 aacatttaga acactttatt gctgataaaa atatgcaaac tcgaactaaa aataagttag
    11041 aggatattgc tttaatatac cgtgagttcg aacaacgcat tcaaaacgag tttattacgg
    11101 gtgaggattc attacaatat tttattgatt gtatgccgaa atcagagtgg ctaaaacgtg
    11161 ctgatatata tattgatggt tttcacaact tttcaacgat tgagtattta ataatcaaag
    11221 gattaattaa atatgcgaag agtgtcacaa ttatattgac gacagatggt aaccacgatc
    11281 aatttagttt atttagaaaa ccatcggaag tgttacgaca tattgaagaa atagcaaatg
    11341 aactcaatat ttctattgaa cgtcaatatt tcaagcaatt atatcgcttc aataatcaag
    11401 atttaaagca tcttgaacaa gaatttgatg cacttcaaat caatcgagtg gcatgtcaag
    11461 gtcatatcaa tattttagaa tctgcgacta tgagagagga aataaatgaa attgcgcgac
    11521 gtatcatcgt tgatattcgt gataagcaat tacgatatca agatattgct attttatatc
    11581 gtgatgaatc ttatgcttat ttatttgatt ccatattacc gctttataat attccttata
    11641 atattgatac aaagcgttcg atgacacatc atccggtcat ggaaatgatt cgttcattga
    11701 ttgaagttat tcaatctaat tggcaagtga atccaatgct acgcttattg aagactgatg
    11761 tgttaacggc atcatatcta aaaagtgcat acttagttga tttacttgaa aattttgtac
    11821 ttgaacgtgg tatatacggt aaacgttggt tagatgatga gctatttaat gtcgaacatt
    11881 ttagcaaaat ggggcgtaaa gcgcataaac tgaccgaaga tgaacgtaac acatttaaac
    11941 aagtcgttaa gttaaagaaa gatgtcattg ataaaatttt acattttgaa aagcaaatgt
    12001 cacaagcgga aactgtaaaa gactttgcaa ctgcttttta tgaaagtatg gaatatttcg
    12061 aactgccaaa tcaattgatg acagagcgag atgaacttga tttaaatggt aatcatgaaa
    12121 aggcggagga aattgatcaa atatggaatg gcttaattca aatccttgat gacttagttc
    12181 tagtatttgg agatgaacca atgtcgatgg aacgtttctt agaagtattt gatattggtt
    12241 tagaacaatt agaatttgtc atgattccac aaacattaga tcaagttagt attggtacga
    12301 tggatttggc taaagtcgac aataagcaac atgtttactt agttggaatg aacgacggca
    12361 ccatgccaca accagtaact gcatcaagtt taattactga tgaagaaaag aaatattttg
    12421 aacaacaagc aaatgtagag ttgagtccta catcagatat tttacagatg gatgaagcat
    12481 ttgtttgcta tgttgctatg actagagcta agggagatgt tacattttct tacagtctaa
    12541 tgggatcaag tggtgatgat aaggagatca gcccattttt aaatcaaatt caatcattgt
    12601 tcaaccaatt ggaaattact aacattcctc aataccatga agttaaccca ttgtcactaa
    12661 tgcaacatgc taagcaaacc aaaattacat tatttgaagc attgcgtgct tggttagatg
    12721 atgaaattgt ggctgatagt tggttagatg cttatcaagt aattagagat agcgatcatt
    12781 taaatcaagg tttagattat ttgatgtcag cattaacgtt tgacaatgaa actgtaaaat
    12841 taggtgaaac gttgtctaaa gatttatatg gtaaggaaat caatgccagt gtatctcgat
    12901 ttgaaggtta tcaacaatgc ccatttaaac actatgcctc acatggtctt aaactaaatg
    12961 agcgaacgaa gtatgaactt caaaactttg atttaggtga tattttccat tccgttttaa
    13021 aatatatatc tgaacgtatt aatggcgatt ttaaacaatt agacctgaaa aaaataagac
    13081 aattaacgaa tgaagcattg gaacaaattt tacctaaagt tcagtttaat ttattaaatt
    13141 cttcagctta ctatcgttat ttatcaagac gcattggcgc tattgtagaa acaacactaa
    13201 gcgcattaaa atatcaaggc acgtattcaa agtttatgcc aaaacatttt gagacaagtt
    13261 ttagaaggaa accaagaaca aatgacgaat taattgcaca aacattaacg acaactcaag
    13321 gtattccaat taatattaga gggcaaattg accgtatcga tacgtataca aagaatgata
    13381 caagttttgt taatatcatt gactataaat cctctgaagg tagtgcgaca cttgatttaa
    13441 cgaaagtata ttatggtatg caaatgcaaa tgatgacata catggatatc gttttacaaa
    13501 ataaacaacg ccttggatta acagatattg tgaagccagg tggattatta tacttccatg
    13561 tacatgaacc tagaattaaa tttaaatctt gggcagatat tgatgaagat aaattagaac
    13621 aagatttaat taaaaagttt aagctgagtg gtttagtgaa tgcagaccaa actgttattg
    13681 atgcattgga tattcgttta gaacctaaat tcacttcaga tattgtacca gttggtttga
    13741 ataaagatgg ctctttgagt aaacgaggca gtcaagttgc agatgaagca acgatttata
    13801 aattcatcca acataacaaa gagaatttta tagaaaccgc ttcaaatatt atggatggac
    13861 atactgaagt tgcaccatta aagtacaaac aaaaattgcc atgtgctttt tgtagttatc
    13921 aatcggtatg tcatgtagat ggcatgattg atagtaagcg atatcgaact gtagatgaaa
    13981 caataaatcc aattgaagca attcaaaata ttaacattaa tgatgaattt gggggtgagc
    14041 aatagatgac aattccagag aaaccacaag gcgtgatttg gactgacgcg caatggcaaa
    14101 gtatttacgc aactggacaa gatgtacttg ttgcagccgc ggcaggttca ggtaaaacag
    14161 ctgtactagt tgagcgtatt atccaaaaga ttttacgtga tggcattgat gtcgatcgac
    14221 ttttagtcgt aacgtttaca aacttaagcg cacgtgaaat gaagcatcgt gtagaccaac
    14281 gtattcaaga ggcatcgatt gctgatcctg caaatgcaca cttgaaaaac caacgtatca
    14341 aaattcatca agcacaaata tctacacttc atagtttttg cttgaaatta attcaacagc
    14401 attatgatgt attaaatatt gacccgaact ttagaacaag cagtgaagct gaaaatattt
    14461 tattattaga acaaacgata gatgaggtca tagaacaaca ttacgatatc cttgatcctg
    14521 cttttattga attaacagag caattgtctt cagatagaag tgatgatcag tttcgaatga
    14581 ttattaaaca attgtatttc tttagcgttg caaatccaaa tcctacaaat tggttggatc
    14641 aattggtgac accatacgaa gaagaagcac aacaagcgca acttattcaa ctactaacag
    14701 acttatctaa agtatttatc acagctgcct atgatgcttt aaataaggcg aatgatttgt
    14761 ttagtatgat ggatggcgtc gataaacatt tagctgttat agaagatgaa cgacgtttaa
    14821 tggggcgtgt tttagaaggt ggctttattg atatacctta tttaactggt cacgaatttg
    14881 gcgcgcgttt gcctaatgta acagcgaaaa ttaaagaagc aaatgaaatg atggtcgatg
    14941 ccttagaaga tgctaaactt cagtataaaa aatataaatc attaattgat aaagtgaaga
    15001 gtgattactt ttcaagagaa gctgatgatt tgaaagctga tatgcaacaa ttggcgccac
    15061 gagtaaagta ccttgcgcgt attgtgaaag atgttatgtc agaattcaat cgaaaaaagc
    15121 gtagcaaaaa tattttagat ttttctgatt atgaacattt tgcattacaa attttaacta
    15181 atgaggatgg ttcgccttca gaaattgccg aatcataccg tcaacacttc caagaaatat
    15241 tggtcgatga gtatcaagat acgaaccgag ttcaagagaa aatactatct tgcataaaaa
    15301 cgggtgcaga acataatggt aatttattta tggttggaga tgttaagcaa tccatttata
    15361 aatttagaca agctgatcca agtttattta ttgaaaagta tcaacgcttt actataaatg
    15421 gagatggcac tggacgtcga attgatttgt cgcaaaactt ccgttctcga aaagaagtac
    15481 tgtcaacgac taactatata ttcaaacata tgatggatga acaagtcggt gaagtaaatt
    15541 atgatgaatc agcacaattg tattatggtg caccatatga tgaatcggac catccagtaa
    15601 acttaaaagt gcttgttgaa gcggatcaag aacatagtga tttaactggt agtgaacaag
    15661 aagcgcattt tatagtagaa caagttaaag atatcttaga acatcaaaaa gtttatgata
    15721 tgaaaacagg aagctataga agtgcgacat acaaagatat cgttattcta gaacgcagct
    15781 ttggacaagc tcgcaattta caacaagcct ttaaaaatga agatattcca ttccatgtga
    15841 atagtcgtga aggttacttt gaacaaacag aagtccgctt agtattatca tttttaagag
    15901 cgatagataa tccattacaa gatatttatt tagttgggtt aatgcgctcc gttatatatc
    15961 agttcaaaga agacgaatta gctcaaatta gaatattgag tccaaatgat gactacttct
    16021 atcaatcgat tgtaaattac attaatgacg aagtagcaga tgctatttta gttgataaat
    16081 taaagatgtt tttatcagat attcaaagtt accaacaata tagtaaagat catccggtgt
    16141 atcagttaat tgataaattt tataatgatc attatgttat tcaatacttt agtggactta
    16201 ttggtggacg tggacgacgt gcaaaccttt atggtttatt taataaagct atcgagtttg
    16261 agaattcaag ttttagaggt ttatatcaat ttattcgttt tatcgatgaa ttgattgaaa
    16321 gaggcaaaga ttttggtgag gaaaatgtag ttggtccaaa cgataatgtc gttagaatga
    16381 tgacaattca tagtagtaaa ggtctagagt ttccatttgt catttattct ggattgtcaa
    16441 aagattttaa taaacgtgat ttgaaacaac cagttatttt aaatcagcaa tttggtctcg
    16501 gaatggatta ttttgatgtg gataaagaaa tggcatttcc atctttagct tcggttgcat
    16561 ataaagctgt tgccgaaaaa gaacttgtgt cagaagaaat gcgattagtc tatgtagcat
    16621 taacaagagc gaaagaacaa ctttatttaa ttggtagagt gaaaaatgat aaatcgttac
    16681 tagaactaga gcaattgtct atttctggtg agcatattgc tgtcaatgaa cgattaactt
    16741 caccaaatcc gttccatctt atttatagta ttttatctaa acatcaatct gcgtcaattc
    16801 cagatgattt aaaatttgaa aaagatatag cacaagttga agatagtagt cgtccgaatg
    16861 taaatatttc aattatatac tttgaagatg tgtctacaga aaccatttta gataataatg
    16921 aatatcgttc ggttaatcaa ttagaaacta tgcaaaatgg taatgaggat gttaaagcac
    16981 aaattaaaca ccaacttgat tatcaatatc catatgtaaa tgatactaaa aagccatcaa
    17041 aacaatctgt ttctgaattg aaaaggcaat atgaaacaga agaaagtggc acaagttacg
    17101 aacgagtaag acaatatcgt atcggttttt caacgtatga acgacctaaa tttctaagtg
    17161 aacaaggtaa acgaaaagcg aatgaaattg gtacgttaat gcatacagtg atgcaacatt
    17221 taccattcaa aaaagaacgc atatctgaag ttgagttaca tcagtatatc gatggattaa
    17281 tcgataaaca tattatcgaa gcagatgcga aaaaagatat ccgtatggat gaaataatga
    17341 catttatcaa tagtgagtta tattcgatta ttgctgaagc agagcaagtt tatcgtgaat
    17401 taccgtttgt agttaaccaa gcattagttg accaattgcc acaaggagac gaagacgtct
    17461 caattattca aggtatgatt gacttaatct ttgttaaaga tggtgtgcat tattttgtag
    17521 actataaaac cgatgcattt aatcgtcgcc gtgggatgac agatgaagaa attggtacac
    17581 aattaaaaaa taaatataag atacagatga aatattatca aaatacgctt caaacgattc
    17641 ttaataaaga agttaaaggt tatttatact tcttcaaatt tggtacattg caactgtagt
    17701 attttgattt tcaaaagaat aaaaaaattt cgattaagtg caaagccctt gtagcagaat
    17761 gaacacaact cattttcaaa attgtcttac ttatttattt gttatttgat aacgaaaaaa
    17821 gttataatgt gaattaagat aaagatgagg agttgagaat gaatgaaatt cttatcattc
    17881 aagtataatg acaaaacttc atatggcgtt aaagtaaaac gcgaagatgc tgtatgggat
    17941 ttaacacaag tatttgctga ctttgcagaa ggagatttcc atcctaaaac attgttagct
    18001 ggtttacaac aaaatcatac tttagatttt caagaacaag tacgtaaagc agttgtagca
    18061 gcagaagata gcggcaaagc tgaagactat aaaatttcat ttaatgacat tgaattctta
    18121 ccaccagtaa cacctccgaa taatgtgatt gcttttggta gaaattacaa agatcatgcg
    18181 aacgaattaa atcatgaagt agaaagatta tatgtattta caaaagcagc gtcatcttta
    18241 acaggagata atgcaacaat tccaaatcat aaagatatta ctgatcaatt agattatgaa
    18301 ggtgaattag gtattgttat tggtaagtct ggtgaaaaga ttccaaaagc attagcttta
    18361 gattatgttt acggctatac aattattaac gatatcactg atcgcaaagc acaaagtgaa
    18421 caagatcaag catttttatc aaaaagttta actggcggtt gcccaatggg tccttatatc
    18481 gttactaaag acgaactacc attacctgaa aatgtaaata ttgttacaaa agttaacaat
    18541 gaaattagac aagatggtaa cactggcgaa atgattctta aaattgatga attaatagaa
    18601 gaaatttcaa aatatgttgc actacatccg ggagatatta ttgcaactgg tacaccagca
    18661 ggcgttggtg caggtatgca accacctaaa ttcttacaac caggtgatga agttaaagtg
    18721 actattgata atattggaac gctgacaact tatatcgcta aataattatc atttaaaaag
    18781 ctaaccagtc tttatataga ttggttagtt ttttgctttt ctaataaggt gttaaagatc
    18841 attgtttata atgttaccat tttgagatga aagtgaaata ttgatattaa gaagtagttg
    18901 attattttac agcagatgca caatattcta ataaggaata aaatgtcatg ttcttcctct
    18961 caaatataga agtgtggtag aatatatatt cgtgtataat caaatcatga ttaaattaca
    19021 agca
//
LOCUS       DAHHOJ010000049        18957 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.049, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000049 DAHHOJ010000000
VERSION     DAHHOJ010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18957)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18957)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18957
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.049"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(94..747)
                     /locus_tag="NBH32_001444"
     CDS             complement(94..747)
                     /locus_tag="NBH32_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HCD1515047.1"
                     /translation="MASLKSIIRQGKQTRSDLKQLRKSGKVPAVVYGYGTKNVSVKVD
                     EVEFIKVIREVGRNGVIELGVGSKTIKVMVADYQFDPLKNQITHIDFLAINMSEERTV
                     EVPAQLVGEAVGAKEGGVVEQPLFNLEVTATPDNIPEAIEVDITELNINDSLTVADVK
                     VTGDFKIENDSAESVVTVVAPTEEPTEEEIEAMEGEQQTEEPEVVGESKEDEEKTEE"
     gene            complement(897..1862)
                     /locus_tag="NBH32_001445"
     CDS             complement(897..1862)
                     /locus_tag="NBH32_001445"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCD1515048.1"
                     /translation="MLNNEYKNSSLKIFSLKGNEALAQEVADQVGIELGKCSVKRFSD
                     GEIQINIEESIRGCDVFIIQPTSYPVNLHLMELLIMIDACKRASAATINIVVPYYGYA
                     RQDRKARSREPITAKLVANLIETAGATRMIALDLHAPQIQGFFDIPIDHLMGVPILAK
                     HFKDDPNINPEECVVVSPDHGGVTRARKLADILKTPIAIIDKRRPRPNVAEVMNIVGE
                     IEGRTAIIIDDIIDTAGTITLAAQALKDKGAKEVYACCTHPVLSGPAKERIENSAIKE
                     LIVTNSIHLDEDRKPSNTKELSVAGLIAQAIIRVYERESVSVLFD"
     gene            complement(2009..3361)
                     /gene="glmU"
                     /locus_tag="NBH32_001446"
     CDS             complement(2009..3361)
                     /gene="glmU"
                     /locus_tag="NBH32_001446"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCD1515049.1"
                     /translation="MRRHAIILAAGKGTRMKSKKYKVLHEVAGKPMVEHVLESVKGSG
                     VDQVVTIVGHGAESVKGHLGERSLYSFQEEQLGTAHAVRMAKSHLEDKEGTTIVVCGD
                     TPLITKETLETLIAHHEDANAQATVLSASIQQPYGYGRIVRNASGRLERIVEEKDATQ
                     AEKDINEISSGIFAFNNKTLFEKLTQVKNDNAQGEYYLPDVLSLILNDGGIVEVYRTN
                     DVEEIMGVNDRVMLSQAEKAMQRRTNHYHMLNGVTIIDPDSTYIGPDVTIGSDTVIEP
                     GVRINGRTEIGEDVVIGQYSEINNSTIENGACIQQSVVNDASVGANTKVGPFAQLRPG
                     AQLGADVKVGNFVEIKKADLKDGAKVSHLSYIGDAVIGERTNIGCGTITVNYDGENKF
                     KTIVGKDSFVGCNVNLVAPVTIGDDVLVAAGSTITDDVPNDSLAVARARQTTKEGYRK
                     "
     gene            complement(3384..3515)
                     /locus_tag="NBH32_001447"
     CDS             complement(3384..3515)
                     /locus_tag="NBH32_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515050.1"
                     /translation="MRLNDARIFEVRKKLFLKLQRIKNNAFYMLKEYCRLNYNNDEV"
     gene            complement(3705..4007)
                     /gene="spoVG"
                     /locus_tag="NBH32_001448"
     CDS             complement(3705..4007)
                     /gene="spoVG"
                     /locus_tag="NBH32_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation regulator SpoVG"
                     /protein_id="HCD1515051.1"
                     /translation="MKVTDVRLRKIQTDGRMKALVSITLDEAFVIHDLRVIEGNSGLF
                     VAMPSKRTPDGEFRDIAHPINSDMRQEIQDAVMKVYDETDEVVPDKNATSEDSEEA"
     gene            complement(4079..4459)
                     /locus_tag="NBH32_001449"
     CDS             complement(4079..4459)
                     /locus_tag="NBH32_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCD1515052.1"
                     /translation="MKIINTTRLPEALGPYSHATVVNGMVYTSGQIPLNIDGHIVSAD
                     VQAQTKQVLENLKVVLEEAGSDLDSVAKATIFIKDMNDFQKINEVYGQYFNEHKPARS
                     CVEVARLPKDVKVEIELVSKIKEL"
     gene            complement(4476..5300)
                     /gene="purR"
                     /locus_tag="NBH32_001450"
     CDS             complement(4476..5300)
                     /gene="purR"
                     /locus_tag="NBH32_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HCD1515053.1"
                     /translation="MRYKRSERIVFMTQYLMNHPNKLIPLTFFVKKFKQAKSSISEDV
                     QIIKNTFQKEKLGTVITTAGASGGVTYKPMMSKEEATEVVNEVITLLEEKERLLPGGY
                     LFLSDLVGNPSLLNKVGKLIASIYMEEKLDAVVTIATKGISLANAVANILNLPVVVIR
                     KDNKVTEGSTVSINYVSGSSRKIETMVLSKRTLAENSNVLVVDDFMRAGGSINGVMNL
                     MNEFKAHVKGVSVLVESKEVKQRLIEDYTSLVKLSDVDEYNQEFNVEPGNSLSKFS"
     gene            complement(5314..6162)
                     /locus_tag="NBH32_001451"
     CDS             complement(5314..6162)
                     /locus_tag="NBH32_001451"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCD1515054.1"
                     /translation="MIYETAPAKINFTLDTLFKRNDGYHEIEMIMTTVDLNDRLTFHK
                     RKDRKIVVEIEHNYVPSNHKNLAYRAAQLFIEQYQLKQGVTISIDKEIPVSAGLAGGS
                     ADAAATLRGLNRLFDIGASLEELALLGSKIGTDIPFCIYNKTALCTGRGEKIEFLNKP
                     PSAWVILAKPNLGISSPDIFKLINLDKRYDVHTKMCYEALENRDYQQLCQSLSNRLEP
                     ISVSKHPQIDKLKNNMLKSGADGALMSGSGPTVYGLARKESQAKNIYNAVNGCCNEVY
                     LVRLLG"
     gene            complement(6471..6734)
                     /locus_tag="NBH32_001452"
     CDS             complement(6471..6734)
                     /locus_tag="NBH32_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HCD1515055.1"
                     /translation="MPKSILDIKNSIDCHVGNRIVLKANGGRKKTIKRSGILKETYPS
                     VFIVELDQDKHNFERVSYTYTDVLTENVQVSFEEDNHHESIAH"
     gene            complement(6834..7727)
                     /gene="rsmA"
                     /locus_tag="NBH32_001453"
     CDS             complement(6834..7727)
                     /gene="rsmA"
                     /locus_tag="NBH32_001453"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCD1515056.1"
                     /translation="MLDNKDIATPSRTRALLDKYGFNFKKSLGQNFLIDVNIINNIID
                     ASDIDAQTGVIEIGPGMGSLTEQLARHAKRVLAFEIDQRLIPVLNDTLSPYDNVTVIN
                     EDILKANIKEAVENHLQDCEKIMVVANLPYYITTPILLNLMQQDIPIDGYVVMMQKEV
                     GERLNAEVGSKAYGSLSIVVQYYTETSKVLTVPKSVFMPPPNVDSIVVKLMQRTEPLV
                     TVDNEEAFFKLAKAAFAQRRKTINNNYQNYFKDGKQHKEVILQWLEQAGIDPRRRGET
                     LSIQDFAKLYEEKKKFPQLEN"
     gene            complement(7738..8274)
                     /gene="rnmV"
                     /locus_tag="NBH32_001454"
     CDS             complement(7738..8274)
                     /gene="rnmV"
                     /locus_tag="NBH32_001454"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HCD1515057.1"
                     /translation="MKINEFIVVEGRDDTERVKRAVECDTIETNGSAINEQTLEVIRN
                     AQQSRGVIVLTDPDFPGDKIRSTITEHVKGVKHAYIDREKAKNKKGKIGVEHADLIDI
                     KEALMHVSSPFDEAYESIDKSVLIELGLIVGKDARRRREILSRKLRIGHSNGKQLLKK
                     LNAFGYTEADVRQALEDE"
     gene            complement(8441..9214)
                     /locus_tag="NBH32_001455"
     CDS             complement(8441..9214)
                     /locus_tag="NBH32_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HCD1515058.1"
                     /translation="MLIDTHVHLNDEQYDDDLSEVITRAREAGVDRMFVVGFNKSTIE
                     RAMKLIDEYDFLYGIIGWHPVDAIDFTEEHLEWIESLAQHPKVIGIGEMGLDYHWDKS
                     PADVQKEVFRKQIALAKRLKLPIIIHNREATQDCIDILLEEHAEEVGGIMHSFSGSPE
                     IADIVTNKLNFYISLGGPVTFKNAKQPKEVAKHVSMERLLVETDAPYLSPHPYRGKRN
                     EPARVTLVAEQIAELKGLSYEEVCEQTTKNAEKLFNLNS"
     gene            complement(9245..11218)
                     /gene="metG"
                     /locus_tag="NBH32_001456"
     CDS             complement(9245..11218)
                     /gene="metG"
                     /locus_tag="NBH32_001456"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCD1515059.1"
                     /translation="MAKETFYITTPIYYPSGNLHIGHAYSTVAGDVIARYKRMQGYDV
                     RYLTGTDEHGQKIQEKAQKAGKTEIEYLDEMIAGIKQLWAKLEISNDDFIRTTEERHK
                     HVVEQVFERLLKQGDIYLGEYEGWYSVPDETYYTESQLVDPQYENGKIIGGKSPDSGH
                     EVELVKEESYFFNISKYTDRLLEFYDQNPDFIQPPSRKNEMINNFIKPGLADLAVSRT
                     SFNWGVHVPSNPKHVVYVWIDALVNYISALGYLSDDESLFNKYWPADIHLMAKEIVRF
                     HSIIWPILLMALDLPLPKKVFAHGWILMKDGKMSKSKGNVVDPNILIDRYGLDATRYY
                     LMRELPFGSDGVFTPEAFVERTNFDLANDLGNLVNRTISMVNKYFDGELPAYQGPLHE
                     LDEEMEAMALETVKSYTESMESLQFSVALSTVWKFISRTNKYIDETTPWVLAKDDSQK
                     DMLGNVMAHLVENIRYAAVLLRPFLTHAPKEIFEQLNINNPQFMEFSSLEQYGVLTES
                     IMVTGQPKPIFPRLDSEAEIAYIKESMQPPATEEEKEEIPSKPQIDIKDFDKVEIKAA
                     TIIDAEHVKKSDKLLKIQVDLDSEQRQIVSGIAKFYTPDDIIGKKVAVVTNLKPAKLM
                     GQKSEGMILSAEKDGVLTLVSLPSAIPNGAVIK"
     gene            complement(11503..12342)
                     /gene="rsmI"
                     /locus_tag="NBH32_001457"
     CDS             complement(11503..12342)
                     /gene="rsmI"
                     /locus_tag="NBH32_001457"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCD1515060.1"
                     /translation="MAVLYLVGTPIGNLADITYRAVDVLKRVDMIACEDTRVTSKLCN
                     HYDIPTPLKSYHEHNKDKQTAFIIEQLELGLDVALVSDAGLPLISDPGYELVVAAREA
                     NIKVETVPGPNAGLTALMASGLPSYVYTFLGFLPRKEKEKSAVLEQRMHENSTLIIYE
                     SPHRVTDTLKTIAKIDATRQVSLGRELTKKFEQIVTDDVTQLQALIQQGDVPLKGEFV
                     ILIEGAKANNEISWFDDLSINEHVDHYIQTSQMKPKQAIKKVAEERQLKTNEVYNIYH
                     QIS"
     gene            complement(12344..12592)
                     /locus_tag="NBH32_001458"
     CDS             complement(12344..12592)
                     /locus_tag="NBH32_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HCD1515061.1"
                     /translation="MDSHFVYIVKCSDGSLYTGYAKDVNARVEKHNRGQGAKYTKVRR
                     PVHLVYQEMYETKSEALKREYEIKTYTRQKKLRLIKER"
     gene            complement(12585..13310)
                     /locus_tag="NBH32_001459"
     CDS             complement(12585..13310)
                     /locus_tag="NBH32_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HCD1515062.1"
                     /translation="MLKENERFDQLIKEDFSIIQNDDVFSFSTDALLLGHFTKPRTKD
                     IVLDLCSGNGVIPLLLFAKHPRHIEGVEIQKTLVDMARRTFQFNDVDEYITMHHMDLK
                     NVTKVFKPSQYTLVTCNPPYFKENQQHQHQKEAHKIARHEIMCTLEDCMIAARHLLKE
                     GGRLNMVHRAERLMDVLFEMRKVNIEPKKVVFIYSKVGKSAQTIVVEGRKGGNQGLEI
                     MPPFYIYNEDGNYSEEMKEVYYG"
     gene            complement(13584..13931)
                     /gene="yabA"
                     /locus_tag="NBH32_001460"
     CDS             complement(13584..13931)
                     /gene="yabA"
                     /locus_tag="NBH32_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HCD1515063.1"
                     /translation="MDRNEIFEKIMRLEMNVNQLSKETSELKALAVELVEENVALQLE
                     NDNLKKVLGNDEPTTIDTANSKPAKAVKKPLPSKDNLAILYGEGFHICKGELFGKHRH
                     GEDCLFCLEVLSD"
     gene            complement(13948..14751)
                     /locus_tag="NBH32_001461"
     CDS             complement(13948..14751)
                     /locus_tag="NBH32_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HCD1515064.1"
                     /translation="MPNVIGVQFQKAGKLEYYTPNDIQVDIDDWVVVESKRGIEIGIV
                     KNPLMDIAEEDVVLPLKNIIRVADDKDIDKFNCNERDAENALILCKDIVREQGLDMRL
                     VNCEYTLDKSKVIFNFTADDRIDFRKLVKILAQHLKTRIELRQIGVRDEAKLLGGIGP
                     CGRSLCCSTFLGDFEPVSIKMAKDQNLSLNPTKISGACGRLMCCLKYENDYYEEVRAQ
                     LPDIGEAIETPDGNGKVVALNILDISMQVKLEGHEQPLEYKLEEIETMH"
     gene            complement(14752..15678)
                     /locus_tag="NBH32_001462"
     CDS             complement(14752..15678)
                     /locus_tag="NBH32_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCD1515065.1"
                     /translation="MDEQQQLTNAYHSNKLSHAYLFEGDDAQTMKQVAINFAKLILCQ
                     TDSQCETKVSTYNHPDFMYISTTENAIKKEQVEQLVRHMNQLPIESTNKVYIIEDFEK
                     LTVQGENSILKFLEEPPDNTIAILLSTKPEQILDTIHSRCQHVYFKPIDKEKFINRLV
                     EQNMSKPVAEMISTYTTQIDNAMALNEEFDLLALRKSVIRWCELLLTNKPMALIGIID
                     LLKQAKNKKLQSLTIAAVNGFFEDIIHTKVNVEDKQIYSDLKNDIDQYAQKLSFNQLI
                     LMFDQLTEAHKKLNQNVNPTLVFEQIVIKGVS"
     gene            complement(15892..16221)
                     /locus_tag="NBH32_001463"
     CDS             complement(15892..16221)
                     /locus_tag="NBH32_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HCD1515066.1"
                     /translation="MKMIIAIVQDQDSQELADQLVKNNFRATKLATTGGFLRAGNTTF
                     LCGVNDDRVDEILSVINQTCGNREQLVSPITPMGGSADSYIPYPVEVEVGGATVFVMP
                     VDAFHQF"
     gene            complement(16249..16866)
                     /locus_tag="NBH32_001464"
     CDS             complement(16249..16866)
                     /locus_tag="NBH32_001464"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCD1515067.1"
                     /translation="MSAFITFEGPEGSGKTTVINEVYHRLVKDYDVIMTREPGGVPTG
                     EEIRKIVLEGNDMDIRTEAMLFAASRREHLVLKVIPALKEGKVVLCDRYIDSSLAYQG
                     YARGIGVEEVRALNEFAINGLYPDLTIYLNVSAEVGRERIIKNSRDQNRLDQEDLKFH
                     EKVIEGYQEIIHNESQRFKSVNADQPLENVVEDTYQTIIKYLEKI"
     gene            complement(16868..18205)
                     /locus_tag="NBH32_001465"
     CDS             complement(16868..18205)
                     /locus_tag="NBH32_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HCD1515068.1"
                     /translation="MKQPILNKLESLNQEEAISLHVPGHKNMTIGHLSQLSMTMDKTE
                     IPGLDDLHHPEEVILESMKQVEKHSDYDAYFLVNGTTSGILSVIQSFSQKKGDILMAR
                     NVHKSVLHALDISQQEGHFIETHQSPLTNHYNKVNLSRLNNDGHKLAVLTYPNYYGET
                     FNVEEVIKSLHQLNIPVLIDEAHGAHFGLQGFPDSTLNYQADYVVQSFHKTLPALTMG
                     SVLYIHKNAPYRETIIEYLSYFQTSSPSYLIMASLESAAEFYKTYDSTVFFDKRAQLI
                     ECLEKKGFEMLQVDDPLKLLIKYEGFTGHDIQNWFMNAHIYLELADDYQALAILPLWH
                     HDDTYLFDSLLRKIEDMILPKKSVSKVKQTQLLTTEGNYKPKRFEYVTWCDLKKAKGK
                     VLARHIVPYPPGIPIIFKGETITENMIELVNEYLETGMIVEGIKNNKILVEDE"
     gene            complement(18831..18945)
                     /gene="rrf"
                     /locus_tag="NBH32_001466"
     rRNA            complement(18831..18945)
                     /gene="rrf"
                     /locus_tag="NBH32_001466"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         5692 a         3812 c         2578 g         6875 t
ORIGIN      
        1 ctttataata acacaaaaag caccatgctt atattaaaat aagcacagtg cttgttaaac
       61 aaagtataaa aactttaatg taacagatta aaattactct tcagtttttt cttcgtcttc
      121 tttgctttcg ccaacaactt ctggttcttc agtttgttgt tcgccttcca tagcttcgat
      181 ttcttcttca gttggttctt cagttggagc aactactgtt actactgatt cagctgaatc
      241 gttttcgatt ttgaagtcgc cagttacttt aacatcagca acagttaagc tgtcgttaat
      301 gtttaattca gtaatgtcta cttcgattgc ttctggaata ttgtctggag tagcagttac
      361 ttctaagttg aataatggtt gttcaactac gccgccttct ttagcgccta ctgcttcacc
      421 aactaattga gctggtactt caacagtacg ttcttcactc atattgattg ctaagaagtc
      481 aatgtgagta atttggtttt taagtggatc gaattggtag tctgcaacca taactttgat
      541 agttttagaa ccaacgccta attcgataac accgttacga cctacttcac ggataacttt
      601 gatgaattct acttcatcaa ctttaactga cacgttttta gtaccgtaac cgtatactac
      661 tgctggtact ttaccagatt ttcttaattg tttaagatct gaacgtgttt gtttaccttg
      721 acggatgatt gactttaatg aagccattct catttccacc tttcattttt gctcaaaacg
      781 agcacctcac ttacccatca acatcatcgt tgcttgcaag tgtttattta ttcgtataat
      841 cacgaacgaa actattatac acgtttggga tatgttcgtc aaacaccttt taaatattag
      901 tcaaataata cgctaactga ttctctttcg tatacacgaa tgatagcttg tgcgattaaa
      961 ccagcaacag ataattcttt agtgttagat ggtttgcggt cttcatctaa atgaattgag
     1021 tttgttacga ttaattcttt tatagcagaa ttttcgatac gttctttagc cggtcctgat
     1081 aaaacagggt gtgtacaaca agcatatact tctttagcac ctttatcttt taatgcttgt
     1141 gcagctaaag tgattgtacc tgctgtatca ataatatcgt caataataat tgccgtacgt
     1201 ccttcaatct caccaacaat gttcatcact tcagcaacat ttggtctagg acgacgttta
     1261 tctataattg caattggagt ttttaaaatg tcagctaatt tacgtgcacg tgtaacaccg
     1321 ccatggtctg gtgaaacaac gacacattct tctgggttaa tattcggatc atctttgaaa
     1381 tgtttagcaa gaattggcac acccattaag tggtcaattg gaatatcaaa gaatccttga
     1441 atttgtggtg catgtaagtc taacgcaatc atacgagttg cgccagctgt ttctattaag
     1501 tttgcaacta atttagctgt gattggctca cggctacggg cttttctatc ttgtcttgca
     1561 tatccataat atggcactac aatattgatt gttgctgcag aagcacgttt acaagcatca
     1621 atcataatta ataattccat tagatgtaga ttcacaggat atgatgttgg ttgaataata
     1681 aatacgtcac aaccacgaat actctcttcg atattaattt gaatttctcc atcactaaaa
     1741 cgtttaactg aacatttacc tagttcaatt cctacttggt cagcaacttc ttgcgctaat
     1801 gcttcgtttc ctttcaatga aaaaatcttt aatgacgaat tcttatattc attatttaac
     1861 atttatagtc ctccaattat ttacttacgc aattactatt atatataaaa tgtaactcca
     1921 ttactatgcg aatcattaaa tcataaagat tacattaata ttacatagga ttatcctttt
     1981 atcctagcca ttttaaatac gtaaatgatt atttcctata tccttctttt gttgtttgtc
     2041 ttgctcttgc cacagctaaa ctgtcatttg gtacgtcatc tgtgattgtg gaaccagctg
     2101 ccaccaatac atcatcacca attgttacag gtgctactaa attaacattg caacctacaa
     2161 atgaatcttt gccgacgata gttttaaatt tattttcacc atcatagtta actgtaatcg
     2221 ttccgcaacc aatattagta cgttcgccaa ttacagcatc gccaatataa cttaaatgtg
     2281 aaaccttggc accatcttta agatctgctt ttttaatttc tacaaaattt ccaaccttaa
     2341 catctgcacc taattgcgcg cctggtctca attgcgcaaa cggtccgacc ttagtattag
     2401 ctcctacgct agcatcatta acaacagact gttgaataca tgcaccattt tcaatcgtac
     2461 tattgttaat ttcagagtac tgaccaataa caacatcttc gccaatttct gtacgaccgt
     2521 taattcgtac gcctggttca atgactgtat cactaccaat tgtaacgtct ggaccaatat
     2581 aagtgctgtc aggatcgatg attgtcacac catttagcat gtgataatga ttcgtacgac
     2641 gttgcatcgc cttctcagcc tgactaagca ttacacgatc atttacaccc atgatttctt
     2701 caacatcatt ggtacgatag acttctacga tgccgccatc atttaaaatt aacgacaata
     2761 catcagggag gtaatattca ccttgcgcat tatcattttt cacttgtgtt aatttttcaa
     2821 acaacgtttt attattaaac gcaaaaatac ctgaactgat ttcattaata tccttttcag
     2881 cttgcgttgc atctttctct tcaactatgc gttctaaacg acctgacgca tttcgaacga
     2941 ttcttccgta tccatatggt tgttgaatcg atgcagataa tacagttgct tgagcattag
     3001 catcctcgtg atgcgcaatc aatgtttcta atgtttcctt tgtgatgagc ggtgtgtcac
     3061 cacatacaac gatcgtcgta ccttccttgt cttctaagtg tgatttcgcc attcgcactg
     3121 catgcgcagt accgagttgt tcctcttgaa aactgtataa agaacgctcg cctaaatgtc
     3181 cttttacact ttcagcacca tgtcctacga tggttacaac ttgatcgaca ccagagcctt
     3241 tcacactttc caatacatgt tcgaccatag gtttcccagc aacctcgtgt agcactttat
     3301 actttttaga tttcattctt gtgcctttac ctgctgccaa aattatcgcg tgtcttcgca
     3361 tgaacattaa cccccattaa attttacact tcgtcattat tataatttaa tctgcaatac
     3421 tctttcaaca tataaaaggc attgttttta attcgttgta attttaaaaa caactttttt
     3481 cttacttcaa aaatacgagc atcattcaaa cgcaattttt atcagcaatg aatcaataat
     3541 tttcatatca tcataatctg ttaacgtata tatttattag cctttcatta aaaataaaaa
     3601 aacattaata caaactaaaa aaactgccga caaagcttta tgctttatcg acagtatcaa
     3661 atcaacttat tgcaaatgta ttacatcgct aaatataaaa ttgattaagc ttcttctgaa
     3721 tcttctgatg tagcgttttt atctggtact acttcatctg tttcatcata tactttcatc
     3781 actgcatctt gaatttcttg tctcatatct gaattaatag gatgcgcgat gtcgcggaat
     3841 tcaccatctg gtgtacgttt acttggcatt gcaacgaaca agccagagtt tccttcaatt
     3901 acacgtaaat catgaattac gaaagcttca tctaatgtaa tggaaacgag tgctttcatt
     3961 cgtccatctg tttgtatttt tctaagtctt acatctgtca ctttcatgta gtgagccccc
     4021 ctatagtata tatatctctg ttttatttag aagcttgatt aaacatatta atcgaaaatt
     4081 ataattcctt aattttactt actaattcaa tttctacttt cacatctttt ggcaaacgcg
     4141 caacctctac acaactacgc gctggcttgt gttcattaaa atattgacca tacacttcat
     4201 ttattttttg gaaatcattc atatctttaa tgaaaatggt cgctttcgca acagaatcca
     4261 aatcagatcc tgcttcttcc aaaacaacct ttaaattttc taaaacttgt tttgtctgtg
     4321 cttgaacatc agcgcttacg atatgtccat caatattcaa tggaatctga ccagaagtat
     4381 aaaccatacc attcacaact gttgcatgcg aatatggtcc aagtgcttcc ggtaatcttg
     4441 ttgtgttaat gattttcata atactaaaac tccttttatg aaaacttaga taaactgttg
     4501 ccaggttcta cgttaaactc ttgattatat tcatctacat cagataattt cactaaggaa
     4561 gtataatctt caatcaatct ttgtttaact tcttttgatt ctacaagtac tgatacccct
     4621 tttacatggg ctttaaactc attcattaaa ttcataacac cattaataga gccaccagcc
     4681 ctcataaaat catcgacaac taaaacattt gaattttctg ctaaagttct cttcgaaagt
     4741 accattgttt ctatttttct tgaagatcct gaaacgtaat taattgaaac tgtagaacct
     4801 tcagtcacct tgttgtcttt tctaatcact actactggta aatttaaaat attagcaacc
     4861 gcatttgcca atgaaatacc ttttgtcgca atggtaacaa cagcatctaa tttttcttcc
     4921 atgtaaatac tggcaattaa cttaccaact ttgtttagta gcgatggatt acctaccaaa
     4981 tctgataaaa ataaatatcc gccaggtaac aaacgttctt tctcttctaa tagagtaatg
     5041 acctcattaa caacttcagt cgcctcttct ttactcatca ttggtttata cgtaacacca
     5101 ccacttgcgc cagcagtagt aattactgta cctaactttt ctttttggaa tgtatttttt
     5161 ataatttgga catcttcact tattgaagac ttcgcctgtt taaatttttt cacaaaaaaa
     5221 gttaatggaa tcaatttatt cggatggttc atcaaatatt gcgtcataaa aacaattctc
     5281 tcgcttcgtt tatatctcat cttttcaacc cttctatcct aatagtctaa ctaagtacac
     5341 ttcattacaa caaccgttaa ctgcattata aatatttttt gcttggcttt cttttcgtgc
     5401 tagcccatac acagtaggtc cgcttccact cattaacgca ccatctgcac cacttttcaa
     5461 catattattt tttaatttat cgatttgtgg gtgttttgaa acagaaattg gctctaatcg
     5521 attagacaaa ctttgacata attgttgata atctcgattt tctaaggcct cataacacat
     5581 tttcgtatgt acgtcgtaac gcttatctaa attaatcaac ttaaatatat ctggtgatga
     5641 tatgcctaag tttggtttag caagaatcac ccaagctgaa ggtggtttat ttaaaaactc
     5701 gattttctct cctcttccag tacatagtgc agttttatta taaatacaaa acggaatatc
     5761 tgtcccgatt ttactgccta gtagagccaa ttcttccaaa ctcgccccta tatcaaaaag
     5821 tcgattcaat cctcttaaag ttgctgctgc atcagccgaa cctccagcta agccagcaga
     5881 aacaggtatt tctttatcga tagaaattgt tacaccttgc tttagttgat attgctcaat
     5941 aaatagttgc gctgcacgat atgcgagatt tttatgatta gaaggcacat aattatgttc
     6001 aatctcaaca actatctttc gatcttttct tttatgaaaa gttaaacgat catttaaatc
     6061 aactgttgtc attatcattt caatctcatg atagccatca tttcttttaa aaagtgtatc
     6121 gagcgtaaaa ttaattttgg ctggtgccgt ttcatatatc attttccgcc ctcttttcgt
     6181 tatcttaata tgatgatttt aacataaaga gaattaatat ttgacatttt aatttgtttt
     6241 ttagtgtttt tttaataaaa aagtaatttt ctccatctaa ttacttagta tttacttgta
     6301 tttgtgttta taaatacaag tatccattta ctgaaaatct ataagcgaaa catcagtgtt
     6361 aaacattttg taaataaaaa gagtattgaa cataatgccc aacactcaaa taattatcat
     6421 aatatttact tcttatacta agaaacgtct aacatctcta tatgtcttat ttagtgtgca
     6481 attgattcgt gatgattatc ctcttcaaat gaaacttgaa cattttcagt taacacatca
     6541 gtgtatgtat aagatactct ctcaaagttg tgtttgtctt gatctaactc aacaatgaaa
     6601 actgacggat atgtttcttt taaaattcca gaacgtttta ttgttttctt acggcctcca
     6661 ttggctttca gtacaatacg atttcctaca tgacaatcaa tagaattttt gatgtccaaa
     6721 attgattttg gcatattgct ccacctcgct acaagttaca taataccata tttgcagcgt
     6781 tttcgtcaaa caaaatttaa attttaacaa tagtgctttg ctttgtcaat catttaattt
     6841 tctaattgag ggaatttttt cttttcttca tacaatttag caaaatcttg aatagatagc
     6901 gtttcaccgc gacgtcttgg atcaatcccc gcttgttcta accattgtaa aatcacttct
     6961 ttgtgttgtt taccatcttt aaaataattt tgatagttat tgttaattgt ctttcttctt
     7021 tgtgcaaatg ctgcttttgc taacttaaag aatgcttcct cgttatctac tgttactaac
     7081 ggttcagttc tctgcatcag ttttacaact attgaatcaa cattaggtgg tggcataaat
     7141 acagatttag gtaccgttaa tactttacta gtctctgtat agtattgtac gacaattgat
     7201 aacgaaccat atgcttttga acctacttca gcatttaagc gttcgcccac ttctttttgc
     7261 atcatcacca cgtagccatc aattggtata tcttgttgca ttaaatttaa taaaattggc
     7321 gtcgtaatat agtacggcag gtttgcaaca accattattt tttcacaatc ttgtaaatga
     7381 ttttcaacag cttctttaat attcgctttt aaaatatctt cattaatcac cgtcacatta
     7441 tcataaggtg atagagtatc atttaataca ggtattaaac gttgatcaat ttcaaatgcc
     7501 aatacccttt tagcatgtct ggccaattgt tctgtcaatg accccatacc tggaccaatt
     7561 tcaatcaccc cagtttgtgc atcaatatca cttgcatcaa tgatattatt aatgatattc
     7621 acatctatca aaaagttctg tcctaaactt tttttaaaat taaagccata tttatctaac
     7681 aacgctcgcg ttcttgatgg tgttgcaata tctttattat ccaatatttt cacttcctca
     7741 ttcatcttct aaagcttgcc ttacatccgc ttcggtataa ccaaatgcat ttaacttttt
     7801 caataactgc ttaccattgg aatggccgat tcgcaatttt ctacttaaaa tttctctacg
     7861 gcgccttgca tctttcccaa cgattaaccc caactctatt agcacagatt tatcaattga
     7921 ttcataagct tcatcaaagg gtgaactaac atgcattaac gcttctttaa tatcaattaa
     7981 gtcggcatgt tcaacaccaa ttttcccttt tttattttta gctttttctc tatcaatata
     8041 cgcatgttta acacctttga catgttcagt aattgtactt ctaattttat ctcctgggaa
     8101 atctggatct gttaatacaa taacgcctcg actttgttga gcatttctaa ttacttctaa
     8161 agtttgttcg ttgatggcac taccattcgt ttcaatcgta tcacattcaa cagctcgttt
     8221 aacacgctca gtatcatctc gtccttctac aactataaac tcattgattt tcatttaaaa
     8281 cgccttccta caattcttaa tttcaaagct ctaaccatct ctatcgcacg tattccacat
     8341 gcacgatatt ttaatttaaa aaagtgagaa agaaccatag catattaaac gaatatagca
     8401 tcgtttacat ttatggcggt gatctttctc acttttaact ttatgaattt aaattaaaca
     8461 atttctctgc atttttagtt gtttgttcgc acacttcttc ataagataag ccttttaatt
     8521 cagcaatttg ttcagctact aaagttactc tcgccggttc atttcgcttc cctctatacg
     8581 gatgtggcga aagatacggt gcatcggttt caactagcaa acgctccatt gacacatgct
     8641 tagcaacttc tttaggctgt ttagcatttt taaatgtcac aggtcctcct aatgaaatat
     8701 aaaaattcag cttattagtt acaatatctg caatttctgg agaaccacta aagctatgca
     8761 taatcccgcc tacctcttca gcatgctcct ccaataagat atcgatacag tcttgagttg
     8821 cttcacggtt atgaatgata attggtaact tcaaacgctt agctaaagca atttgctttc
     8881 taaaaacttc cttttgaaca tctgcaggag atttatccca gtgataatct aatcccattt
     8941 caccaatacc aatcactttt ggatgctgag ctaaagattc aatccattcc aagtgttctt
     9001 ctgtaaaatc aattgcgtca actggatgcc aaccgataat gccatataaa aaatcatact
     9061 catcgattaa tttcatcgcg cgttcaattg tcgatttgtt aaaaccaact acaaacatac
     9121 gatcaacacc tgcttctcta gcacgtgtaa tcacttcact caaatcatca tcgtattgct
     9181 catcatttaa atggacatgt gtatcgatta acataattat ctctcctaat ttttaaaaat
     9241 acagttattt aatcactgca ccatttggaa ttgcacttgg taaacttact aaggttaata
     9301 caccatcttt ttcagcagat aatatcatac cttcagattt ttgtcccatt aatttagctg
     9361 gtttcaggtt agtaacaact gctacttttt taccaataat atcatctggt gtatagaatt
     9421 tggcaattcc tgatacaatt tgtctttgtt cagaatctaa gtctacttga atttttaaaa
     9481 gcttatctga cttcttaaca tgttcagcat caataatcgt tgctgcctta atttcaactt
     9541 tatcaaagtc tttaatatca atttgaggtt tgctaggaat ctcttctttt tcctcttcag
     9601 tagcaggcgg ttgcattgat tctttgatat atgcaatttc cgcttcgcta tccaatcttg
     9661 ggaaaatagg tttaggttgc ccagtaacca taattgactc agtaagcaca ccatattgct
     9721 ctaaactact aaattccata aattgaggat tgtttatgtt caattgttca aaaatctctt
     9781 tcggcgcatg tgttaagaat ggacgtaata atacagctgc ataacgaata ttttcaacta
     9841 agtgagccat tacattgcct aacatatctt tttggctatc gtccttagct aatacccaag
     9901 gcgttgtttc gtcaatatac ttattcgttc tactaataaa cttccatacc gtagataatg
     9961 ccacagaaaa ttgcaaactt tccatgcttt cagtgtagct tttcactgtt tctaaagcca
    10021 tagcttccat ttcttcatct aattcatgaa gtggaccttg atacgctggt aattcgccat
    10081 caaagtactt attaaccata gaaatcgtac ggtttactaa gttacctaag tcatttgcta
    10141 gatcgaaatt tgtacgctca acaaatgctt caggtgtaaa tacgccatct gaaccaaatg
    10201 gtaattcacg cattagataa taacgtgtag catctaaacc atagcgatca attaaaatat
    10261 tagggtctac gacattacct ttagatttac tcatttttcc atctttcatc aaaatccaac
    10321 catgtgcaaa gactttttta ggtaacggta agtctaatgc catcaataaa ataggccaaa
    10381 taattgagtg gaatcgcaca atttccttag ccattaaatg aatatctgct ggccagtatt
    10441 tgttaaatag tgactcatca tctgataaat agcctaatgc tgaaatatag ttaactaacg
    10501 catcaatcca aacataaaca acatgttttg gattagacgg aacatggaca ccccagttaa
    10561 atgatgtacg agaaacagct aaatcagcaa gtcctggttt aatgaagttg ttaatcattt
    10621 cattttttct tgatggtggt tgtataaaat ctgggttttg gtcatagaat tctaatagac
    10681 ggtctgtata tttactaata ttaaagaaat aactttcttc tttaactagt tcaacttcgt
    10741 gtccagaatc tggacttttg ccaccaataa ttttaccgtt ttcgtattgt gggtctacta
    10801 attgtgactc tgtatagtat gtttcatccg gaacagaata ccaaccttca tattcaccta
    10861 aatagatatc accttgcttt aataaacgtt caaacacttg ctcaacgaca tgtttatgac
    10921 gttcttcagt tgttctgata aaatcatcat ttgaaatttc aagcttagcc cacaattgtt
    10981 taattccagc aatcatctca tccaaatatt caatttctgt cttaccagct ttttgagctt
    11041 tttcttgaat tttttgaccg tgttcatccg ttccagtcaa ataacgaaca tcatatcctt
    11101 gcattctctt atatcttgca ataacatctc cagccactgt agaatatgca tgtcctatat
    11161 gtaaattccc actaggatag tatattgggg ttgttatata aaatgtttct ttagccataa
    11221 ctgttcctcc tcgtaatgtc tcacttcaaa taatatctaa gatttctatg catttcaacg
    11281 gacgcgaaca tttatctcgt catctcattt tcatcttatt caatgttaag gtatttatta
    11341 ctttcaatgt atttatttta cattaaaaaa cgtcgatgtc atcatttttt tatcatagtc
    11401 aacatcattt tatcagtatc aatgcaaaat gataacttac catttaaaat tacagcttgc
    11461 gtttataaaa tcgtttaaaa tttcatataa tcgataaagt gattaactta tttgatgata
    11521 aatattatat acttcattcg ttttaagttg tcgttcttca gcaacttttt taatagcttg
    11581 ttttggtttc atctgtgaag tttgaatata atgatcaaca tgctcattga tagataaatc
    11641 atcaaaccac gatatctcat tgttcgcttt agcaccttca attaagataa cgaattcgcc
    11701 tttcaatggt acatcgcctt gctgaatcaa tgcttgtaat tgtgttacat catcagttac
    11761 aatttgttcg aacttcttag ttaattcacg ccctagtgat acttgtcgtg ttgcatctat
    11821 ctttgcaatt gtttttaatg tatctgtcac acgatgcggt gattcgtata taattaatgt
    11881 gctattttca tgcatacgtt gctctaatac agcacttttt tctttctctt ttcgtggcaa
    11941 aaatcctaaa aatgtatata catatgaagg taatccacta gccatcaaag ccgtcagccc
    12001 agcattaggt ccaggcacag tctctacttt aatattagct tctctggctg ccactactaa
    12061 ttcgtatcca ggatcactaa ttaagggcaa tccagcatca gatacgagcg caacgtcaag
    12121 acctaattct aactgttcaa tgataaaagc agtctgctta tccttgttat gttcgtgata
    12181 tgactttaat ggagttggaa tatcataatg attacacagc ttactagtta ctctagtgtc
    12241 ttcacaagca atcatatcaa cacgtttcaa tacatcaact gctctataag taatatctgc
    12301 taaattacca attggtgtgc ccactaaata taatacagcc atactatcgc tccttaatta
    12361 atcgcaattt cttttgtctg gtataagttt taatttcata ttcacgcttc aatgcttcag
    12421 actttgtctc atacatttct tgataaacta aatgcaccgg acgtcttact ttcgtatatt
    12481 tggctccttg acctcggtta tgtttttcaa cacgtgcatt aacgtcttta gcgtatcctg
    12541 tatataaact tccatcacta cattttacaa tatatacaaa atgactatcc ataatatact
    12601 tccttcattt cttcgctata attaccatct tcattataaa tataaaatgg gggcatgatt
    12661 tctaaacctt gatttccacc ttttcgacct tctactacta tcgtttgtgc tgatttccct
    12721 actttactat atataaaaac gactttctta ggttcaatat tcacttttct catttcaaac
    12781 aagacatcca ttagtctctc tgcacgatgt accatgttta gcctgccacc ttcttttaat
    12841 aaatgacggg ctgcaatcat gcaatcttca agtgtacaca taatctcatg tctcgctatc
    12901 ttatgtgctt ctttttgatg ttggtgttgc tgattctctt taaaataagg tggattacac
    12961 gttactaaag tatattgtga aggtttaaat actttagtaa cgtttttcaa atccatgtga
    13021 tgcattgtta tatattcatc aacatcattg aattgaaatg tgcgtcgcgc catatcgaca
    13081 agtgtttttt gaatctcaac accttctata tgtcgtggat gtttcgcaaa caataacaac
    13141 ggtatcaccc cattgcctga acataagtcc aacacaatat cttttgttct aggttttgta
    13201 aaatgcccta acaacaaagc atccgttgaa aacgaaaaaa catcatcatt ttgaataata
    13261 ctaaaatctt ctttgattag ttgatcaaat cgttcattct cttttaacat ttttaactcc
    13321 tatgaagaca acctttttct attgagtaat tgaaatctct ttttatatta tatgcctatc
    13381 tacattcgat gtatttttga cttttaacag ctacatttta taactaatga tactgcgtgc
    13441 acacttaata ataacattta gtaccgctat ctcttatgca atcaccaaaa ctttatacac
    13501 aattaaagcc tgaaacatgc attgtctcag acttatccaa accatattca tttataatta
    13561 taacactatt tgagtgtgct tgattaatca cttaaaactt ctaaacagaa cagacaatct
    13621 tcaccatgtc gatgttttcc aaataattcg cctttacaaa tatgaaatcc ttctccatac
    13681 aatatagcca aattatcttt acttggtaat ggctttttca cagcttttgc tggttttgaa
    13741 ttcgcagtat caatagtagt tggttcatca ttgcccaaca cctttttcaa attatcattt
    13801 tcaagttgaa gcgctacatt ttcttctact aattcaactg caagtgcctt taattctgaa
    13861 gtttcttttg aaagttgatt gacattcatt tctaaacgca ttattttttc aaatatttca
    13921 ttgcgatcca aatgtaataa tgcctcctta atgcatagtt tctatttctt ctaatttata
    13981 ttcaagtggc tgttcatgtc cctcaagctt cacctgcata gaaatgtcta atatatttaa
    14041 agcaactact ttcccgttac catcaggcgt ttcaattgct tcaccaatat caggtaattg
    14101 tgcacgtact tcctcataat agtcattttc atattttaaa caacacatca aacgaccaca
    14161 tgcaccagaa attttagttg gatttaatga taaattttga tccttagcca tcttaatcga
    14221 tactggttca aaatccccta aaaatgtaga acaacataac gacctaccac aaggtccgat
    14281 accgccaagc aatttggctt catcccttac accaatttgt ctcaactcga tacgtgtttt
    14341 taaatgttgc gctaatattt ttactaattt tctaaaatca atacgatcat ccgccgtaaa
    14401 attaaaaata actttcgatt tatctaatgt atattcgcaa ttgactaaac gcatgtccaa
    14461 accttgttct cttacaatgt ctttacatag tattaatgca ttttcagcat ctcgttcatt
    14521 acaattaaat ttatcaatat ctttgtcatc agcaacgcga ataatatttt taagaggtaa
    14581 cacaacatcc tcttcagcaa tatccattaa tggattttta acaataccta tctctatgcc
    14641 tcttttagat tcgacaacta cccagtcatc tatatctact tgtatatcat taggtgtata
    14701 atattctaat tttcccgctt tttgaaactg aacacctatt acatttggca tctaactcac
    14761 acccttaatt acgatttgtt caaatacaag cgttggattt acattttgat tcaatttctt
    14821 atgtgcttcc gtcagttgat caaacatcaa aattaattga ttaaacgaca acttttgcgc
    14881 atattgatca atatcatttt ttaaatcact atatatttgt ttatcctcta catttacctt
    14941 tgtatgtatg atatcttcga agaaaccatt cacagctgca atagttaaag attgcagttt
    15001 tttattttta gcctgtttca ataaatcaat aatacctata agtgccattg gcttattagt
    15061 aagcaacaat tcacaccaac gtataactga tttccttaat gctaataaat caaattcttc
    15121 atttaaagcc attgcattat ctatttgcgt agtataagta ctaatcattt cagctactgg
    15181 cttagacatg ttttgttcaa ctaatctatt tataaacttt tctttatcaa taggcttgaa
    15241 atatacatgc tgacaccttg aatggattgt gtctaaaatt tgctcaggtt ttgtagacaa
    15301 taaaatagca atcgtattgt ccggtggttc ttcaagaaat ttcaagatac tgttttcccc
    15361 ttgaacagtt aacttttcaa agtcttcaat gatgtacact ttatttgtgc tttctatagg
    15421 aagttgattc atatgacgca caagttgttc aacttgttct ttcttaattg cattctcagt
    15481 tgttgatata tacataaagt ctggatgatt atatgtacta acctttgttt cacattgact
    15541 atctgtttga cataaaataa gctttgcaaa attaatcgca acttgtttca tcgtttgtgc
    15601 atcatcacct tcaaataaat aggcatgcga taatttattt gaatgatatg cattcgtcaa
    15661 ttgttgctgt tcatccattc attacaaact cccttttgct tttatataaa aaggttgcca
    15721 aagagcaact aattactgca acaatagcga ccatgatttg tgattatttt tagaaccaca
    15781 aaaatcaaat ctattggcat ctgaactagt cgcagtcaag caacccaaaa ttgttaataa
    15841 cttattaata cactatttta agtatctcta attgatgatt gtattataga attaaaattg
    15901 atggaatgca tcaactggca taacaaatac agtagcaccg ccaacttcaa cttcaactgg
    15961 atatggaatg tacgaatccg cactgcctcc cataggtgta ataggtgaaa ccaactgttc
    16021 tctattacca cacgtttgat taatcacaga caatatttca tctacacggt catcattgac
    16081 accacataag aatgttgtat tacccgctct taaaaaccca cctgttgttg ccaattttgt
    16141 tgctctaaag ttatttttaa caagttgatc tgcaagttcc tgactatctt gatcttgtac
    16201 gatcgctata atcattttca ttttataaca cctcttctaa caattatatc atatcttttc
    16261 taaatatttg atgatagttt gatacgtgtc ttcaacaaca ttttcaagag gttgatctgc
    16321 attaacgctt ttgaaccgtt gtgattcatt atgaatgatt tcttggtaac cttcaattac
    16381 tttttcgtga aactttaaat cttcttgatc taatctattt tgatctcttg aatttttaat
    16441 aatacgttcg cgacctactt cagcactaac atttaaataa atcgtcaagt ctggatataa
    16501 tccatttatt gcaaattcgt ttaatgctct tacttcttca acgccaatcc ctctagcata
    16561 accttgataa gctaatgaac tatcgatata gcgatcacac aacacaacct taccttcttt
    16621 taaagctggt atgaccttta atacaagatg ttctcttcta gatgcagcaa ataacattgc
    16681 ttcagttcta atgtccatat cattgccttc taatacaatt ttacgtattt cttcaccagt
    16741 aggaacacca cctggttctc tagtcataat gacatcataa tcttttacta atctatggta
    16801 aacttcatta attacagttg tttttccaga gccttctggg ccctcaaaag ttataaaagc
    16861 tgacatttta ttcatcctca actaaaattt tattattttt aattccttca actatcattc
    16921 cagtttccag atattcattt accaattcta tcatattttc agttattgtt tctcctttga
    16981 aaataatagg aatccctggc ggatacggga caatatgtcg cgccagaact ttaccttttg
    17041 cctttttcaa atcacaccaa gtaacatatt caaagcgttt tggtttatag ttaccttcag
    17101 ttgttaaaag ttgtgtttgt ttaactttag aaactgattt tttcggtaaa atcatatctt
    17161 caattttacg taaaagcgaa tcaaataaat acgtatcatc atgatgccat aacggcaata
    17221 tcgctaatgc ttgatagtcg tccgctaatt ctaaatagat atgtgcattc ataaaccaat
    17281 tttgaatatc atgacctgta aaaccttcat attttatcag caacttcaac ggatcatcaa
    17341 cttgaagcat ttcaaaaccc ttcttctcca aacattcgat taattgcgct ctcttatcaa
    17401 aaaacacggt actatcatat gttttataga actcggcagc tgactctaaa ctagccataa
    17461 tcaaatacga aggactagat gtttggaagt agcttagata ttctataata gtttctctat
    17521 aaggtgcatt tttatgaata taaagtaccg agcccatcgt taaagctggt aacgttttat
    17581 gaaaagattg aacaacatag tcagcttgat aatttaatgt agaatctgga aatccttgca
    17641 atccaaagtg cgcgccgtgt gcttcgtcaa tgagtacagg aatatttaat tggtgcaaag
    17701 atttgataac ctcttctaca ttaaatgttt caccgtaata gttaggataa gtcaacacag
    17761 caagtttgtg accgtcatta ttcaaacggc ttaaattaac tttattataa tgattcgtta
    17821 acggactttg atgcgtttca ataaaatgcc cttcttgttg gctaatatcg agcgcatgta
    17881 acacagattt atgtacattt cttgccatta agatatcgcc ttttttctgt gaaaaagact
    17941 ggatgacaga taatattcct gaagtggtgc cattcactaa gaaataagca tcataatctg
    18001 aatgtttctc cacctgcttc atactttcca aaatgacttc ttcaggatga tgtaaatcat
    18061 ctaatccagg tatttcagtt ttatccattg tcattgataa ttgagataaa tgaccgatag
    18121 tcatattttt atgacccgga acatgcaaag aaatcgcttc ttcttgattt aaactttcta
    18181 atttatttaa aataggttgc ttcatgatat acgcttcctt tatttacact gttttggaat
    18241 tagttacttt caaaagtatt aattatatag taacacttct ttgacaaaag ttagtgttac
    18301 ttatgcaata gcttgtctat tgtataataa ttaatttcct ttttgtactt cgatttaaaa
    18361 gatattagac ataaaatcta aaaacagcag taagatgatt tatgattaaa aactatctta
    18421 ctgctgttca ctttttataa tacttctgaa tgtcttcact tatacttcta gtcacagatt
    18481 taaataatca aaagtgcaca ttattaaaat atcaatttca cactcaatgc ggctcatcgc
    18541 attcatttct tgtctagcaa cgttctactc tagcggaacg taagttagct accatcctcg
    18601 ctaagaacct ttcttgactt gtgacaatcg cttgcttctt tcctctcctt cggctctcgc
    18661 ttactcattt agctctacta aactcgttgc gctcttttct cgtttcgtca gattcaaacg
    18721 ttttcacttc gccaagccat ttttctttgt gtttactttt tattttgacg ttttagacat
    18781 aaaaaaaaga gaccttgcgg tctcaatgcg gctcatcgca tccatttttt gcctggcaac
    18841 gttctactct agcggaacgt aagttcgact accatcgacg ctaaggagct taacttctgt
    18901 gttcggcatg ggaacaggtg tgacctcctt gctatagtca ccagacatat gaatgta
//
LOCUS       DAHHOJ010000050        18575 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.050, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000050 DAHHOJ010000000
VERSION     DAHHOJ010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 18575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.050"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            175..918
                     /gene="sarU"
                     /locus_tag="NBH32_001467"
     CDS             175..918
                     /gene="sarU"
                     /locus_tag="NBH32_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarU"
                     /protein_id="HCD1515069.1"
                     /translation="MDYQTFEKVNKFINVEAYIFFLTQELKQQYKLSLKELLILAYFY
                     YKNEHSISLKEIIGDILYKQSDVVKNIKSLSKKGFINKSRNEADERCIFVSVTPIQRK
                     KIACVINELDKIIKGFNKERDYIKYQWAPKYSKEFFILFMNIMYSKDFLKYRFNLTFL
                     DLSILYVISSRKNEILNLKDLFESIRFMYPQIVRSVNRLNNKGMLIKERSLADERIVL
                     IKINKIQYNTIKSIFTDTSKILKPRKFFF"
     gene            complement(1130..1996)
                     /gene="galU"
                     /locus_tag="NBH32_001468"
     CDS             complement(1130..1996)
                     /gene="galU"
                     /locus_tag="NBH32_001468"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000721336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCD1515070.1"
                     /translation="MKKIKKAIIPAAGLGTRFLPATKAMPKEMLPILDKPTIQYIVEE
                     AARAGIEDIIIVTGRHKRAIEDHFDSQKELEMVLKEKGKSELLEKVQYSTELANIFYV
                     RQKEQKGLGHAISSARQFIGNEPFAVLLGDDIVESEVPAVKQLIDVYEETGHSVIGVQ
                     EVPEADTHRYGIIDPLTKNGRQYEVKKFVEKPAQGTAPSNLAIMGRYVLTPEIFDYLK
                     TQKEGAGNEIQLTDAIERMNNDNQVYAYDFEGERYDVGEKLGFVKTTIEYALKDDSMR
                     EELTRFIKELGL"
     gene            complement(2176..5223)
                     /gene="fnbA"
                     /locus_tag="NBH32_001469"
     CDS             complement(2176..5223)
                     /gene="fnbA"
                     /locus_tag="NBH32_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000794614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein FnbA"
                     /protein_id="HCD1515071.1"
                     /translation="MKNNLRYGIRKHKLGAASVFLGTMIVVGMGQDKEAAASEQKTTT
                     VEENGNSATENKVNETQTTTTNVNTIDETQSYSATATEQPSNATQVTTEEAPKAVQAP
                     QTAQPANLETVKEEVVKEEAKPQVKETTQSQDNSGDQRQVDLTPKKATQNQVAETQVE
                     VAQPRTASESKPRVTRSADVVEAKEASDEKVETGTDVTSKVTVESGSIEAPQGNKVEP
                     HAGQRVVLKYKLKFADGLKRGDYFDFTLSNNVNTYGVSTARKVPEIKNGSVVMATGEI
                     LGNGNIRYTFTNEIEHKVEVTANLEINLFIDPKTVQSNGEQKITSKLNGEETEKTIPV
                     VYNPGVSNSYTNVNGSIETFNKESNKFTHIAYIKPMNGNQSNTVSVTGTLTEGSNLAG
                     GQPTVKVYEYLGKKDELPQSVYANTSDTNKFKDVTKEMNGKLSVQDNGSYSLNLDKLD
                     KTYVIHYTGEYLQGSDQVNFRTELYGYPERAYKSYYVYGGYRLTWDNGLVLYSNKADG
                     NGKNGQIIQNNDFEYKEDTAKGTMSGQYDAKQIIETEENQDNTPLDIDYHTAIDGEGG
                     YVDGYIETIEETDSSAIDIDYHTAVDSEAGHVGGYTESSEESNPIDFEESTHENSKHH
                     ADVVEYEEDTNPGGGQVITESNLVEFDEESTKGILTGAVSDHTTVEDTKEYTTESNLI
                     ELVDELPEEHGQAQGPIEEITENNHHISHSGLGTENGHGNYGVIEEIEENSHVDIKSE
                     LGYEGGQNSGNQSFEEDTEEDKPKYEQGGNIVDIDFDSVPQIHGQNKGDQSFEEDTEK
                     DKPKYEHGGNIIDIDFDSVPHIHGFNKHTEIIEEDTNKDKPNYQFGGHNSVEYEEDTL
                     PQVSGHNEGQQTIEEDTTPPTPPTPEVPSEPETPTPPTPEVPSEPETPTPPTPEVPSE
                     PETPTPPTPEVPAEPGKPVPPAKEEPKKPSKPVEQGKVVTPVIEINEKVKAVAPTKKA
                     QSKKSELPETGGEESTNKGILFGGLFSILGFALLRRNKKNHKA"
     gene            complement(5207..5309)
                     /locus_tag="NBH32_001470"
                     /pseudo
     CDS             complement(5207..5309)
                     /locus_tag="NBH32_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501263.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(5692..7050)
                     /locus_tag="NBH32_001471"
     CDS             complement(5692..7050)
                     /locus_tag="NBH32_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease"
                     /protein_id="HCD1515072.1"
                     /translation="MFNEIWPLISVVLGIVILLVLIIGFKLNTFISLIITSMITALML
                     GIPLTKIMETIEKGMGSTLGHIALIFGLGAILGKLLADGGGATRIADTLIQKFGQKHV
                     QWAMLVAAFIVGIALFFEVGLVLLIPLVFTVAKRANVSVLKLGLPMVTALSVTHGFLP
                     PHPGPVVIAKELKANVGDVLLYGMIIAIPVTLIAGPIFNKVAQKMIPSAYTREGDISA
                     LGAQKEFTDQEMPGFGMSLLTATLPVILMLVSTITQLVTGHDKPTNLFESIIYMIGTA
                     GTAMLIAVLFAIVTMGLMRKRKMNHIMESVTNAIYPIGMMLLIIGGGGTFKQVLIDGG
                     VGNTIAKMFEGTEMSPILLAWIVAAVLRIALGSATVAAISTTGIVLPLLQSSDVNVAL
                     VVLAIGAGSVILSHVNDAGFWMFKEYFGLTVKETFLTWSLLETIISVSGIIFILFISL
                     FV"
     gene            complement(7167..8720)
                     /gene="gntK"
                     /locus_tag="NBH32_001472"
     CDS             complement(7167..8720)
                     /gene="gntK"
                     /locus_tag="NBH32_001472"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCD1515073.1"
                     /translation="MKYMIGVDIGTTSTKSVLYDDNGTFIMKHQIGYDLHTPNVDVSE
                     ENPDELFDAVLMTIKYVMRESKVNKDDIKFVSFSAQMHSLIAMDQKHQRLTNNITWAD
                     NRAAKYATVINEVHDGNAIYQRTGTPIHPMSPLVKIFWMKHEWQDVFQRTAKFADIKT
                     YIFYHLFDTYIIDYSMASATGMFNLETLDWDVGALELLGISKEMLPELVPTTYVMKGM
                     KERYATLMGLNEDTPFVIGASDGVLSNLGVNSVGKGEVAVTIGTSGAIRTVIDKPRTD
                     YKGRIFCYVLTEDHYVIGGPINNGGVVLRWLRDELLASEVETAKRLGVDPYDVLTQIA
                     KRVKPGADGLIFHPYLAGERAPLWNANARGSFFGLTLSHKKEHMIRAALEGVLYNLYT
                     VYLALIEVMNETPKMIKATGGFAKSEVWRQMMSDIFDTELVVPESYESSCLGACVLGL
                     KAVGDIEDFSIVSSMVGATNNHMPFEENVAVYQELVSIFINLSRSLTENYEQIADFQR
                     QHMAENKTQ"
     gene            complement(8744..9424)
                     /locus_tag="NBH32_001473"
     CDS             complement(8744..9424)
                     /locus_tag="NBH32_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1515074.1"
                     /translation="MMYGYPEKWLEGMTTGEGIAAELRLGIVNGHIAEGTLLTENQMA
                     KQFNVSRSPIRDAFKLLQQNQLIQLERMGAHVLPFGEQEKKEMYDLRLMLESFAFSRV
                     KNQERLPIVKEMKKQLEMMKVAVKFEDAESFTKHDFEFHETLIKASNHQYLNSFWSHL
                     KPVMMALVLTSMRQRMQQNPQDFERIHHNHQVFIDAVEQYDSQILKEAFHLNFDDVGK
                     DIEGFWLN"
     gene            complement(9578..10342)
                     /locus_tag="NBH32_001474"
     CDS             complement(9578..10342)
                     /locus_tag="NBH32_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1515075.1"
                     /translation="MSNYSTGELAKLCNVTTRTIQYYDRKGILKPQGFTEGKRRVYTE
                     QQRQTLELILLLKDLGCALSDIDMLLKGEGTLKTLNTLLTMKQQEINQQVKQQQAVLN
                     KIKNVQYYVNKASTSPITHLKDIEHVMSKSAEMKSIRRNIWISAGIIGIIQYSSIISA
                     ILMKNKWPFLIALPFMIGYGIGVTFYYQQKVAYLCPNCQHIFSPSLWAVIKAKHTATT
                     RRFECPNCHETHYCIEVPKAHMSTEQLEISRIQHNN"
     gene            complement(10507..11199)
                     /locus_tag="NBH32_001475"
     CDS             complement(10507..11199)
                     /locus_tag="NBH32_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HCD1515076.1"
                     /translation="MYVDRKPSLYLEDLRHDFKNSLSKFENGDEAFDTLLGFVELDHI
                     YSSALKEISTKLSILDDNFNHIYKHNPIHHMERRVKEMRSLIEKLNRKGLQISAETAK
                     EHILDIAGIRVVCNYLDDIYLIEEMLLKQEDVQLIKRKDYIQHPKENGYRSLHIVVSI
                     PVFLAERVEVLPVEIQIRTIGMDMWASLEHKIRYKNNAETEKYRDLLKECATEITEVE
                     DKLQQIHSEITE"
     gene            complement(11430..11846)
                     /locus_tag="NBH32_001476"
     CDS             complement(11430..11846)
                     /locus_tag="NBH32_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HCD1515077.1"
                     /translation="MPFTMKGYPEKWKDFEVIKRQKAIEIANAMLKEGYLEKDVVPIA
                     AKKAKDWYRALSKEEIKALEEENFVQFHTATMEKTEVDSEYATTDNEEHIQEQSNQDK
                     SYFDSESIGNQSGGVRQREPLMDGPHPKVVESFKDV"
     gene            complement(11985..13808)
                     /locus_tag="NBH32_001477"
     CDS             complement(11985..13808)
                     /locus_tag="NBH32_001477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000586397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCD1515078.1"
                     /translation="MIIPAIIGYLCGNFQFGLLVATGTLAHIYVFKGPSRSKLRTVII
                     CNLAFAICMMLGTLTAKTPLVFGMTLLIVTVIPFYIFTALKIAGPSSTFFIVTFSLPI
                     NLPIAPEEALYRGFAILVGGILATMMVLITIVFSKNKAEEQAIQNDFKLISKLLHTYN
                     DKSAFLQVAKTAVDNFKASDKLLITSTSSNDKLSRRFQKLLLLHTSAQGIYSELLELN
                     AKQIRPLPDELIEMMDHIIAQLDNSEENVRYWRKEVTVTEEFQNLFNHILKIDEMVHA
                     NEARIAYEADMRKPLYSKRIYQNLTLDSIVFRNTLRYTAIMMIAIFIALMFDFEKAYW
                     IPLSAHTILLGTSTIHAIERGMARGLGTILGVLVLSVILLFSIPTPVAVILMGIAALF
                     TEALVGANYAIAVVFITIQVILMNGLASQNLTINIAFPRVIDVAMGIVIAIIGLFVLG
                     QRTASALLPNVMAEVVRKEATLFHYLFSENQYKDDVYQKNTAMNLSVKLNNMTQVYNA
                     ANGELFSDKTLIQNYYPSLFALEEISFMLNRAMANEDRLTINEQLMGEYLATFENIAK
                     HLELNTELEIKILPDLPQYNYIQSAMMNIQHNSFCERDKNV"
     gene            complement(14718..15995)
                     /locus_tag="NBH32_001478"
     CDS             complement(14718..15995)
                     /locus_tag="NBH32_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515079.1"
                     /translation="MKEKRTNVRWMFALAFFFIGVIAYMDRANISYIAKQMMDDLGMT
                     KPQFGLLASFFSLGYALMQVPSGMLAEKFGPRKMITIALVWWSAFTILTGMIKNHGLI
                     YLVRFLFGVGEAPMYPSNAVFNSFWFSKNEKGRASSALLAGSYFGPVLAPIVTIAIVN
                     AFNWQAVFYIFGAVGILMAVLWAIIAKDLPEQHRMVNEAEKRFIMENRDIVATEKSSP
                     PWNDFFKRFSFYAIAIQYFVVQFIITLFLIWLPTYLTEVFHVNFKEMSISSLPWLLMF
                     FLILSAGAISDRVLGLGRSKFVARGVIAIAGFIVFAVSIIFAVRTGNLYVSIFWLSLG
                     LGGIGISMGMSWAAATDLGRNFSGTVSGWMNLWGNIGALISPLLAGLFVEHLGWTMTF
                     QLLIVPAVIAVIMWFYVKPDQPLIVSDDKAIEK"
     gene            16542..17153
                     /locus_tag="NBH32_001479"
     CDS             16542..17153
                     /locus_tag="NBH32_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCD1515080.1"
                     /translation="MEQIITEFISRFGYAAIFILILLENVLPIVPSEIILTFAGLMSV
                     KSHLSILTLFIIATIASFIGLLILYYICRLISEERLYRFIDRHGKWIKLKSKDLKRAN
                     DWFKKYGVWAVFICRFIPVLRVLITIPAGVNRMNVVTFTVISLIGTTIWNFGLILLGR
                     TLSDSFGMLMTGLHTYSRIMYVIIIIAVIYFAIRYIGKRKRVK"
     gene            complement(17162..17261)
                     /locus_tag="NBH32_001480"
                     /pseudo
     CDS             complement(17162..17261)
                     /locus_tag="NBH32_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501275.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(17251..18414)
                     /locus_tag="NBH32_001481"
     CDS             complement(17251..18414)
                     /locus_tag="NBH32_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515081.1"
                     /translation="MTNSNIRLYILGFLAFFASLIQNIYTPIIPRLYDDFQVPLFWIN
                     ATVGGFIFIVAVMQIVLGRSIDSRDSKKVLLTGLGIVIISSFICAMTHNFILFAISRL
                     FQAIGCGIIPLVTLTLLAKLSTDNGRAQAMANYQIFLSCAPALAPILGSTLGARWDYI
                     GIFSFLLVISIVLFLIIFFIDIPNVEKGIVKLTEKIEEKYLIDKVFITLVTLGFLIFL
                     TYFSILVYLPTLLNNTYDIGVGISGVLFLPITVSVILGSLFYKRLSKKYNEIMILRVT
                     ITGFAIFTLLFGWLNESNVIILSVIIFILGTCVGIVPALLSTIISKRFEHIKGKVLGV
                     FNFVRYIGMTVGALLIGIISHSLVPFYFTTVTIMLIIIFLYIKIGDFQLKFAK"
     gene            complement(18407..>18575)
                     /locus_tag="NBH32_001482"
     CDS             complement(18407..>18575)
                     /locus_tag="NBH32_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1515082.1"
                     /translation="EGKKLSYIHDELHEKAVKKYEEVLKVFDNDEMAVIVEFLNRSIE
                     ELKKEEDGLND"
BASE COUNT         5761 a         3490 c         2659 g         6665 t
ORIGIN      
        1 tttggataat aaatgaattt gtcatttgtt attccgtgat ttttagttat ttatatttta
       61 tatatattat atataaaaat atttaggtta acatgtcttc aaaagagatt gcttttatat
      121 tactttaaat aatataaagt attgggacat cagaaaatag taggagaatg taaggtggac
      181 tatcaaactt tcgaaaaggt caataaattc ataaatgtag aagcgtacat attttttctc
      241 actcaagagt taaagcaaca atataaatta tcattaaaag aattgttgat attagcatat
      301 ttttattaca aaaatgaaca cagtatttca ctaaaagaaa tcattggtga catactttac
      361 aaacaatctg atgttgtaaa gaacattaag tcactatcta aaaaaggatt tataaataag
      421 tctagaaacg aagcagatga acgctgtatt tttgtttcag ttactccaat acaacgtaaa
      481 aagattgctt gtgttattaa tgagttagat aaaataatta aaggatttaa taaggaaaga
      541 gactacataa aatatcaatg ggctccaaaa tatagcaaag aattttttat actttttatg
      601 aacattatgt actcaaaaga ttttttaaaa tatcgattta atttaacatt tcttgattta
      661 tctatcttat atgtaatatc atctcgaaaa aatgagatac taaatttaaa agatttgttt
      721 gaaagtatta gatttatgta tcctcaaatt gttaggtcag ttaatagatt aaataataaa
      781 ggtatgctaa tcaaagaacg atcccttgca gatgaaagga ttgtgttaat caaaataaat
      841 aaaatacaat ataacactat taaaagcata ttcacagata cttccaagat tctcaaacca
      901 agaaaatttt tcttttaaat ttaaacagat ttacctcttg ataaaataaa taagcaatca
      961 taatacttct caatttagta taaataaaaa tacataatta actttctttt gtttttatat
     1021 tatttcaata ccctactata tatcacaaca cataaattaa gcatgacact cattcaattt
     1081 agttcacaat ttcgtgttcc aattttattg agtatcatgc tttttaatgt tataacccta
     1141 attctttaat aaatcgtgtt aattcttctc gcatactgtc atctttcaat gcatattcta
     1201 tcgttgtttt aacgaagcct aatttttctc caacgtcata acgttcgcct tcgaagtcat
     1261 atgcatacac ttggttatca ttattcatac gttcaatcgc atctgttaac tgaatttcgt
     1321 tacctgcgcc ttctttttgc gtttttaaat aatcgaaaat ttcaggcgtt aatacataac
     1381 gtcccataat agctaggttt gatggtgccg taccttgtgc tggcttttca acaaactttt
     1441 tcacttcata ctgacgtccg tttttagtta atgggtcaat aatcccataa cgatgagtat
     1501 ctgcttccgg aacttcttgg acacctataa ctgagtgccc tgtttcttca taaacgtcaa
     1561 tcaactgttt cactgctggc acttcagatt caacaatatc gtcacctaat aataccgcaa
     1621 atggttcatt gccgataaat tgacgcgcag aactaatcgc atgccctaaa cctttttgtt
     1681 ctttctgcct tacataaaaa atattcgcaa gttctgttga atactgaact ttttctagta
     1741 actcagattt acctttttct tttaacacca tttctaattc tttttgacta tcaaaatgat
     1801 cttcaatcgc gcgtttgtgg cgacctgtca ctataataat atcttcaatt ccagctcttg
     1861 cagcttcttc aacgatatat tgtattgtgg gtttatctaa gataggaagc atttcctttg
     1921 gcatcgcttt agttgctggt aaaaatctag tccctaaacc agcagcggga atgattgcct
     1981 tttttatttt tttcaaagtt aatgtgctcc ttttcctaag tattaaatct atgtatcaac
     2041 gtcattttaa cactaattag aacgccttca tagtgtcatt aagtatgtaa ttatttcttg
     2101 ggaaatttga tttaatttta aaaaacaggc ttacttcata taatttatga aataaacctg
     2161 tcaattttag attgattatg ctttgtgatt ctttttattt ctgcgtaata atgcaaatcc
     2221 taaaatacta aacaatcctc cgaacagtat acctttgttt gttgattctt ctccacctgt
     2281 ttcaggtagt tcagatttct tagattgtgc ttttttagtt ggtgccactg ctttaacctt
     2341 ttcattgatt tcaatgacag gtgttactac cttaccttgt tccactggtt tagaaggctt
     2401 tttaggttct tctttggcag gtggtactgg tttaccaggt tcagctggta cctctggcgt
     2461 tggtggtgtt ggtgtttccg gctcacttgg tacctctggt gttggcggtg ttggtgtttc
     2521 cggctcactt ggtacttctg gtgtcggtgg tgttggtgtt tccggctcac ttggtacttc
     2581 tggtgttggt ggtgttggcg gcgttgtatc ttcttcaatc gtttgttgac cttcattatg
     2641 accacttact tgtggaagtg tatcttcttc atattcaaca ctattgtgtc caccgaattg
     2701 ataatttggt ttatctttgt ttgtatcttc ttcaataatt tcagtgtgct tattgaatcc
     2761 atgaatatgt ggcacgctgt cgaagtcgat atcaatgata ttaccaccat gttcatattt
     2821 aggcttgtct ttctctgtat cttcttcgaa tgactgatca cctttatttt gaccatgaat
     2881 ttgaggtaca ctatcgaaat cgatatctac gatattgcca ccttgttcat atttaggttt
     2941 atcttcttct gtgtcttcct cgaatgactg gttaccgcta ttttggccac cttcataacc
     3001 taattcactc ttaatatcaa cgtggctatt ttcttcgatt tcttcaatca caccataatt
     3061 cccgtgacca ttttcagttc ctaaaccaga atgagaaata tgatgattgt tttcagtaat
     3121 ttcctcgatt ggtccttgtg cttgaccatg ctcttcaggt aattcatcta ctagttcaat
     3181 cagattactt tcagtcgtat attctttcgt atcttcaact gttgtatggt cgctcactgc
     3241 gccagttaga ataccttttg tagactcttc gtcaaattca actaagttag actcagttat
     3301 tacttggcca ccacctgggt ttgtatcctc ttcatattca acaacatcag cgtgatgttt
     3361 tgaattttca tgtgtcgatt cttcaaagtc aattggattt gattcctcag aggactcagt
     3421 gtatcctcca acgtgacccg cttcactatc cacagcagta tggtaatcga tatcaatagc
     3481 tgatgaatcc gtttcttcta ttgtttcaat atacccatca acataaccac cctcaccatc
     3541 tatagctgtg tggtaatcaa tgtcaagcgg tgtattgtct tgattttctt ctgtttcaat
     3601 aatttgcttg gcatcgtact gcccgctcat agttcctttt gcagtatctt ctttatattc
     3661 aaaatcatta ttttgaataa tttgtccatt tttaccattg ccgtcagctt tattgctata
     3721 taaaactaaa ccattatccc aagttaaacg atatccccca taaacatagt aagatttata
     3781 tgctcgttct ggatacccat ataattcagt tctaaaatta acctgatctg acccttgcaa
     3841 atattcacct gtataatgaa tgacatacgt tttatccaac ttatctaaat tcaatgagta
     3901 actaccattg tcttgcacac tcaattttcc attcatttcc tttgttacat ctttgaattt
     3961 gttagtatct gatgtatttg cataaacact ttgtggcaat tcatcttttt tccctagata
     4021 ttcatatact ttaacagtag gttgtccacc agctaaatta ctaccttcag tcaacgtccc
     4081 tgttactgat acagtgtttg actggtttcc attcattggc ttaatataag ctatatgtgt
     4141 aaatttatta gattctttat taaatgtttc aattgatcca tttacatttg tataactatt
     4201 gctaacacct ggattataaa caactggtat tgttttttct gtttcttcac catttaattt
     4261 agaagtaatc ttttgttctc cattgctttg tacagtttta gggtcaataa ataagttgat
     4321 ttctaaatta gctgttacct ctaccttgtg ttcaatttcg ttagtaaatg tatatcttat
     4381 gttaccattc cctaagatct cacctgtagc cattacaact gagccatttt taatctctgg
     4441 tacctttcta gctgttgaaa ccccataagt atttacatta tttgataatg taaaatcaaa
     4501 ataatctcct ctttttaatc catctgcgaa tttcaatttg tatttcaata cgacacgttg
     4561 accagcatgt ggctctactt tatttccttg aggtgcctca atagaaccac tttctactgt
     4621 aactttactt gtcacatctg tacccgtttc cactttctcg tcactagctt ccttagcttc
     4681 aactacgtct gctgatcttg tcacacgtgg tttactttct gatgccgttc ttggctgtgc
     4741 cacttcaact tgtgtttctg cgacttgatt ttgtgtggcc tttttaggtg ttaaatctac
     4801 ttgtctttga tctccgctat tgtcttgaga ttgtgttgtt tccttaactt gaggtttcgc
     4861 ttcttcctta actacctctt ctttaactgt ttctaaattt gctggttgtg cagtttgtgg
     4921 tgcttgtact gcttttggtg cttcttcagt tgttacttgt gttgcgtttg acggttgttc
     4981 tgttgctgtt gcgctgtatg attgtgtttc atctatagta ttaacgttag ttgtagttgt
     5041 ttgtgtttcg tttactttat tttcagtagc tgaattccca ttttcttcta ctgtagttgt
     5101 cttttgttct gatgctgcag cttctttatc ttgtcccatc ccaacaacga tcattgttcc
     5161 taagaatact gatgctgctc ccaatttatg ttttctaatg ccgtacctaa gattgttttt
     5221 cactataata tctcccttta aatgcaaaat tcattaattt ttttaaactt aataaatgca
     5281 agtctatatt gttcggtttt taaaagcaat acaaaaaagc gagttaatga aaagttaaga
     5341 ttgtcattaa ttttatgtta attgataatt ttattatttg aaatatacct ataaattgta
     5401 ttcaagtcat cataaaccct tgtcacacaa ggcttgtatt ttttatactt atttttcaaa
     5461 ttaaattcat cattatctaa tttaaaacaa tatactaagc gtttcataat tatcgcctgt
     5521 acaatacgca caaaaacatg tcttgaaacg cctttcatta cactaaaatc cccaatatac
     5581 tttttatatc gttcggattc tgactatttc agacgatttt ctgcataaaa ataaacgtgt
     5641 ttcaaggcaa tatattgcaa ttaccttaaa acacgtttac ttaatattta gttaaacaaa
     5701 taagctgatg aatagaatga agatgatacc tgaaacggaa ataatcgttt ctaataatga
     5761 ccatgttaag aatgtttctt ttacagttaa accaaaatat tctttaaaca tccaaaatcc
     5821 tgcgtcattt acatgagaca aaatcacact acctgcacct atcgcaagta caactaatgc
     5881 aacatttaca tctgatgatt gtaataatgg taagacaata cctgtagttg aaatcgcagc
     5941 tactgtagcc gaacctaatg cgatacgtag cacagctgca acaatccatg ctagtaaaat
     6001 tggagacatc tctgtacctt caaacatttt agcaattgta tttccgacac caccgtcaat
     6061 taatacttgt ttaaatgtac cgccaccgcc aataatcaat aacatcattc cgattggata
     6121 aatcgcattc gtcactgatt ccataatatg attcatctta cgttttctca ttaatcccat
     6181 cgtaacgatt gcaaataata ctgctattag catggctgtc cctgctgttc ctatcatata
     6241 aatgatagat tcaaatagat ttgtaggttt gtcgtgccca gttacaagtt gcgttatcgt
     6301 agacactaac attaatatga ctggtaatgt tgcagttaat aaactcatac caaatcctgg
     6361 catctcttga tccgtaaatt ctttttgtgc acctaacgct gaaatatcgc cttctcgtgt
     6421 atacgcagac ggaatcattt tttgtgcaac tttgttaaat ataggtcctg caatgagtgt
     6481 aactggaatg gcaataatca taccgtacag taatacatct ccaacatttg cctttaattc
     6541 ttttgcgatg actaccggtc ctggatgtgg tggtaaaaag ccatgtgtca cagataaagc
     6601 tgttaccata ggtagtccta gttttaacac tgaaacattt gcgcgttttg ctactgtaaa
     6661 tactaatgga atcagtaaga ctaaacctac ttcaaagaac aatgcaatac cgacgataaa
     6721 tgctgcaaca agcattgccc attgtacatg tttttgacca aatttttgaa tcaacgtgtc
     6781 tgcaattcga gttgcaccac caccatcagc aagtaatttc ccaagtatgg cacctaaacc
     6841 gaatattagt gcaatgtggc cgagcgtact gcccatccct ttctcaatcg tctccataat
     6901 tttagtcaat ggtataccta gcattaacgc tgtaatcatc gatgtgataa ttaatgaaat
     6961 aaatgtattt aatttaaacc caataattaa tactaataaa ataacgatac ctaaaacaac
     7021 actgattaac ggccatattt cgttaaacat gacattcccc tctttctctt ttcaatagaa
     7081 tgtaacaccg tcgtcgcgca cgtattccac ataatatata gtagtcatgg cacaacaact
     7141 ttatctaaga tgcttaagtg acgtatttat tgtgttttat tttcagccat atgttggcgt
     7201 tgaaaatctg caatttgttc ataattctct gttaaagaac gacttaaatt gataaaaatg
     7261 gatacgagct cttggtaaac agcgacattt tcttcaaacg gcatatgatt atttgtagca
     7321 ccgaccatcg atgaaacgat tgaaaaatct tcaatgtcac ctacagcttt aagtccaagc
     7381 acgcaggcac ctaagcatga actttcatag ctttcaggaa ccactaactc tgtgtcaaat
     7441 atatctgaca tcatttgacg ccatacttca cttttcgcaa aaccacctgt tgcttttatc
     7501 atcttaggcg tttcattcat tacttcaatg agtgcaaggt agacggtata caaattgtaa
     7561 agaacacctt ctaatgcagc gcgaatcata tgttcttttt tatgagataa agttaaaccg
     7621 aagaatgaac ctcttgcatt tgcgttccaa agcggtgcac gttctccagc taaataggga
     7681 tggaatatta aaccatctgc acctggttta acacgctttg caatttgagt taagacatca
     7741 taaggatcaa caccgagacg tttcgcagtt tcgacttcac tcgctagcaa ctcgtcgcgc
     7801 aaccatctca atacgacacc accattattt ataggacctc cgatgacgta gtggtcctct
     7861 gttaagacat aacaaaatat tctacctttg taatcagtac gcggtttatc tatcacagta
     7921 cgaatcgccc cagatgtacc gattgtgaca gcaacttctc ctttaccaac gctattgaca
     7981 cccaaattag aaaggacccc atcactcgca ccaataacaa acggtgtatc ttcattaagc
     8041 cccattaatg ttgcataacg ttctttcatg cctttcatta cgtacgttgt tggcactaat
     8101 tccggcaaca tttccttgga aataccaagc aattctaatg ccccaacatc ccaatctaat
     8161 gtttctaaat taaacatccc tgttgcggaa gccattgaat aatcaatgat atatgtatca
     8221 aataaatgat agaaaatgta tgttttaata tctgcaaact tagcagtacg ttgaaataca
     8281 tcttgccatt catgtttcat ccaaaaaatt ttcactaaag gcgacatagg atgaataggc
     8341 gtacctgttc gctgataaat cgcattgcca tcatgcactt catttattac tgttgcatat
     8401 tttgcagcgc ggttatctgc ccaagtaata ttatttgtta atctttgatg tttctgatcc
     8461 atcgcaatca agctatgcat ttgcgcacta aatgacacaa atttaatatc atctttatta
     8521 actttggatt ctctcataac atatttaata gtcattaata ccgcatcaaa taattcatct
     8581 gggttttctt ctgagacatc aacgtttggt gtgtgtaaat catagccgat ttgatgtttc
     8641 atgatgaatg ttccattgtc atcatataag actgacttgg tactcgtcgt tccaatgtcg
     8701 acaccaatca tatatttcat gacaaatcct tctttctttc attttaattc aaccaaaatc
     8761 cttcaatatc tttaccaaca tcgtcgaaat ttaagtgaaa cgcttctttc aaaatttgac
     8821 tgtcgtattg ttccactgca tcaataaaca cttgatgatt atgatgtatg cgttcaaaat
     8881 cttgcgggtt ctgttgcata cgttgtcgca tggatgttaa aacgagtgcc atcattactg
     8941 gttttaaatg actccaaaat gagtttaaat attgatgatt agatgctttg attaatgttt
     9001 catgaaattc aaagtcatgc ttcgtaaatg attctgcatc ctcaaatttt actgccactt
     9061 tcatcatttc aagttgtttc ttcatttctt ttacgatagg tagtcgctct tgatttttaa
     9121 ctcttgaaaa tgcaaaagac tctagcatca gtcgcaaatc gtacatttct ttcttttctt
     9181 gttccccaaa cggcaacaca tgtgcaccca ttctttctaa ttggatgagt tgattttgtt
     9241 gcaataattt aaatgcatct cgaattggcg aacgactcac attaaattgc tttgccattt
     9301 gattttcagt gagtaacgta ccttcagcta tgtgaccatt cacaatacct aagcgtaatt
     9361 ctgccgcgat accttctcca gttgtcatac cttccaacca tttctctgga tatccataca
     9421 tcatcaaagc cactccttca ttacacgaca tacttgtata caagtatgtt aatatagttc
     9481 ttaatcgttt gcaagcgctt tctttacgcg tactaaaata gcgaccaccc cttttcgatg
     9541 taagtttaaa gggaatggtc actatcacac gaataattta attgttatgt tgtatgcggg
     9601 atatttctaa ttgttctgta ctcatatgcg ctttaggtac ttcaatgcaa taatgcgttt
     9661 catgacagtt cggacattcg aatcgacgtg ttgtcgctgt atgtttcgct ttgataactg
     9721 cccacaaaga tggtgagaat atatgctggc agttaggaca taaataggca accttttgtt
     9781 ggtaataaaa agtaacacca atgccgtatc caatcataaa cggcaacgca attagaaacg
     9841 gccatttatt tttcatcaaa attgcactta taatgctaga atattgaatt attcctataa
     9901 tacccgcact aatccaaatg ttacgacgaa tacttttcat ttcagctgat ttactcatga
     9961 catgctctat gtcttttaaa tgtgtgattg gagacgtcga cgctttattt acgtaatatt
    10021 gaacattttt aattttgttt aataccgctt gttgctgttt gacttgttga ttaatttctt
    10081 gttgtttcat agttagtaaa gtattgagtg tcttcaaagt accttcacct tttagcaaca
    10141 tatctatatc gcttaacgca caacctaaat ctttaagcaa taagattaac tctaatgttt
    10201 gtcgctgttg ttctgtatac acacgacgct ttccttctgt aaatccttgt ggtttcaaaa
    10261 tacctttgcg atcataatat tgaatcgttc gcgttgtcac attgcataat ttcgcgagtt
    10321 ctccagtcga atagttagac atagattcca cctcctacaa tcaccatagt tgatgacccg
    10381 acgtcacgag caagtacttt ttctacattt taaagaaatt tattatacta ggcgtcttat
    10441 ttttgtgatt tcgtaccatg ttgatttaca aaatcactca aattaagcaa cactactact
    10501 aaacgtctac tctgttattt cagaatgaat ttgttgtaat ttatcttcaa cttcagtaat
    10561 ctctgtcgca cattctttca gtaaatctcg atacttttcc gtctctgcat tgtttttata
    10621 acgtatttta tgttctaaac ttgcccacat atccatacct atcgttctaa tttgaatttc
    10681 aacaggcaat acctcaacgc gttctgctaa aaagactgga atggatacaa cgatatgtaa
    10741 actgcggtaa ccattttctt tagggtgctg aatataatct ttacgtttta tcaattgtac
    10801 gtcttcttgt ttaagcaaca tctcttcaat caaataaata tcatctaagt aattacatac
    10861 tacgcgaatt ccggcaatat ccagtatatg ttctttggca gtttctgcgc taatctgtaa
    10921 tcctttacga ttaagctttt ctattaaact gcgcatttct ttcacacgtc gctccatatg
    10981 atgtataggg ttgtgtttat aaatgtgatt gaaattatcg tctaaaatac tcagtttagt
    11041 gcttatttcc tttagtgctg acgaataaat atgatctaac tctacgaaac ctaataacgt
    11101 atcaaatgct tcatcaccat tttcaaattt acttaaacta tttttaaaat catgtcgcaa
    11161 atcctctaaa tatagtgatg gttttcgatc tacatacatt tttatactaa cctccgatat
    11221 ataatcatct ttattgtacc taacatttta ttaagataat actaatacac tatcgaactt
    11281 tgggctgata ctgaaacagc acaaagaaac tcaaacaatt cgaattatgt atcaaaacct
    11341 tcaattatag gaagtaattt taaaatgatt taacgcaaaa aaacacctgt taccgttata
    11401 taggtacaag tgtttaattg atagagatgt tatacgtctt taaatgattc cacaactttt
    11461 ggatgtggac catccataag cggttctctt tgtcgaacgc caccactttg gttgccaatt
    11521 gattcgctat caaagtacga tttatcttga tttgattgtt cttgaatatg ttcttcatta
    11581 tcagttgttg catattcact atccacctct gttttttcca ttgttgctgt atggaattgt
    11641 acgaagttct cttcttctaa tgctttgatt tcttctttcg ataacgctcg ataccaatct
    11701 ttcgcttttt tcgccgcaat aggaacaaca tctttttcaa gatatccttc ttttaacatt
    11761 gcattcgcaa tttctatcgc tttttgccgt ttaattactt caaaatcttt ccatttttcg
    11821 gggtaacctt tcattgtaaa gggcatttcc ttaacctcca atatgttata attcatgtta
    11881 tataccacca taattataaa atgaaacata ttaaacacaa ttttttagac tcccgtcata
    11941 ataacttcag acaaacgtta gtctgacctg ataaatattt tattttaaac gttcttgtct
    12001 ctctcacaaa aactattgtg ttgaatattc atcattgctg attgaatata attatactgt
    12061 ggtaaatctg gtaatatctt tatttctaat tctgtattta attctaaatg cttagcgatg
    12121 ttttcaaatg ttgctaaata ctcgcccatt aattgttcat ttattgtaag tctatcttca
    12181 ttagccatgg ctctatttaa cataaagcta atttcttcta atgcgaataa gctaggataa
    12241 taattttgaa tcaatgtctt atcactaaat aattcaccat tagctgcatt ataaacttgc
    12301 gtcatgttat ttaatttcac agataggttc atagccgtgt ttttctgata cacatcatct
    12361 ttatattggt tttcagaaaa caaataatgg aagagcgttg cttctttacg aacaacttca
    12421 gccattacat taggaagcaa tgcggatgcg gtacgttgtc caaggacaaa taaacctatg
    12481 attgcaatca caatacccat tgcaacgtca ataactcttg gaaacgcaat gttaattgtt
    12541 aaattctgtg atgctaatcc attcattaaa ataacttgta ttgtaataaa aactactgca
    12601 atcgcataat ttgctcccac caatgcttca gtaaacaatg ctgcaatgcc cattaaaatt
    12661 actgcaacag gtgttggtat tgaaaacaac aatatgactg aaagtactaa cacacctaaa
    12721 atagtaccta aacctcgtgc catacctctc tcgattgcat gtatagttga tgttcctagt
    12781 aatattgtat gtgcagataa cggtatccag tatgcttttt caaaatcaaa cattaacgca
    12841 ataaatatcg ctatcatcat aatcgctgta tatctcaatg tatttctaaa aacaatagag
    12901 tctaatgtta aattttgata aatgcgttta ctatataaag gttttcgcat gtctgcttca
    12961 tacgcaatac gcgcttcatt tgcatgcacc atttcatcaa ttttcaatat atggttgaat
    13021 aaattttgaa attcctctgt tactgtcact tcttttcgcc aatatcttac attttcctca
    13081 ctattatcta gttgtgcaat gatatgatcc atcatttcaa ttaactcatc tggcaatggt
    13141 cgaatttgtt tagcgttcaa ctctaacagt tcagaataaa tcccttgggc agatgtgtgt
    13201 aataataata atttttggaa acgtctactt aatttatcgt tacttgaagt agaagtgatt
    13261 aataatttat cagatgcttt aaaattatcc accgctgttt ttgctacttg taaaaatgca
    13321 gatttatcat tataagtgtg taacaactta gatatgagtt taaaatcatt ttgaattgct
    13381 tgttcttcag ctttgttttt agaaaatacg atcgtgatta acaccatcat agtggcaagt
    13441 ataccgccta ctaaaatcgc aaagcctcta tataatgctt cttcgggagc tataggtaag
    13501 ttaatgggta gactgaatgt cacaatgaag aatgtcgatg acggtccagc tatttttaag
    13561 gcagtaaata tataaaatgg tataaccgta acaattaata atgtcattcc aaaaacgagt
    13621 ggcgttttgg ctgttagcgt accaagcatc atacatattg caaacgctaa attacaaatt
    13681 attacagttc gcagcttaga tcgcgacgga cctttaaaaa cataaatatg ggctagtgtt
    13741 ccggttgcaa ctaataatcc aaattggaaa ttaccacata agtaaccgat tattgcggga
    13801 ataatcatta acagtccctg tcttacacct ttataaaagt ctaagttaag tcgattgatt
    13861 tttatgagtg atgtcaaaaa gatgtcattt cgacttctcc tttccttgtc aaaatatttc
    13921 cataatcttt ttattagcat agttaaaatg aatttgttgc tataaatttt aattacgcaa
    13981 catatgtcat tataacacct tatgctcaat tatctaaatt taaaaacttt taaagtccaa
    14041 ttaaaattaa tttctaatgt tatttaacat cgttgaacct ttggtcgccc acctttgcgc
    14101 tagttaaaat ttatttgtga catgttgaaa ttattttagc gaattataca tttagggtta
    14161 gaaaaacatt cacatagact tagtgtctat ttacttcata ttattcctag ggttcaagat
    14221 ccaaactata gtaaatttaa gaaagtttat aaaacatatt tgcttcataa gcttgaatag
    14281 ttcctatatt cgttatttaa aatgacattc gatgtcagtt cattttttaa aatatcgaaa
    14341 gatgcttacg tataatatat atagtgacgt atttacaaat aatggcagta agcagtacaa
    14401 aatgattaca caaaaatttc cttctactcg ataaattaag aagaataaac taattaaaaa
    14461 aatacttata attagaccta taagaggcaa acttctaata tatttctcta agttttcctt
    14521 catatttata acctcctaaa atttatatgt acttttataa gcgttacctg actaatgttt
    14581 taaaacttta tttctttaca tacatcattt taataacttc tctttcatct taaaccatat
    14641 ctgttttaaa aagtttcaat ttcaacgtaa aaaaagcaat aagccccaac aatgtgaaag
    14701 cttactgctt gtttaaatta tttttctatt gctttatcat cactaacaat taaaggttga
    14761 tctggtttca catagaacca cataatcaca gcgattactg ctggaacgat taacaattgg
    14821 aatgtcattg tccaacccaa atgttctacg aatagacctg ctaatagcgg actgattaat
    14881 gcacctatat taccccataa gttcatccac cctgatactg taccagagaa gttacgtcct
    14941 aagtcagttg ctgcagccca actcataccc attgaaatac cgataccacc aagacctagt
    15001 gataaccaga aaatacttac atataaattt cctgtacgta cagcaaagat aattgaaact
    15061 gcaaacacaa taaatcctgc gattgcaatt acacctctag ctacgaattt tgaacgacct
    15121 aatcctaata cacggtcaga aattgcacct gctgataaga ttaagaagaa cattaataac
    15181 caaggtaatg aactaatgct catttcttta aagttaacgt ggaatacttc tgttaaatac
    15241 gtcggtaacc aaattaagaa taatgtaatg ataaattgta caacaaagta ttgaattgca
    15301 attgcataga agctaaaacg cttaaagaaa tcattccatg gtggtgatga cttttcagta
    15361 gctacgatat cacgattttc cataatgaaa cgtttctccg cttcattaac cattctatgt
    15421 tgctcaggta agtctttggc aataatcgcc cataatacag ccattaaaat acctactgca
    15481 ccaaaaatgt aaaatactgc ttgccagtta aatgcgttaa caatagcaat tgtaactatt
    15541 ggtgctaata caggtccgaa atatgatcct gctaataatg cacttgatgc tctacctttt
    15601 tcatttttag agaaccaaaa tgagttaaac acagcattag aagggtacat tggcgcctca
    15661 cctacaccaa ataagaatct cactaaataa attaaaccgt ggttcttaat catacccgtt
    15721 aagattgtaa atgcactcca ccaaactaat gcaattgtaa tcatcttacg tggaccgaat
    15781 ttttcagcca acatccccga tggtacttgc attaatgcat aacctagaga gaagaatgat
    15841 gccaataaac caaattgtgg ctttgtcatc cctaaatcat ccatcatttg cttagcgata
    15901 tatgaaatgt ttgctctatc catatacgca ataaccccaa taaagaagaa cgcaagcgcg
    15961 aacatccacc ttacgttagt tcgtttttcc ttcataacta ttcctacttc cctaattaat
    16021 tttttaaaac ttaccctgaa acgttaaaat tttcggacat ctggcatgtc cacccctact
    16081 ttgtcacaaa attgtaccaa agttattttt tataatatta tcagacatgg aaagcgttta
    16141 caatagaatc tataaaaatt ttattaaatt ccgcttaact tttgctatta aaacgatata
    16201 aaggaacttt tagttacata gtagcctatc atttgttaat taaaaatact gcaatgctag
    16261 tgttttaaaa cgtatattgc aggctttctt acatatttga cattcatttt aaattgcata
    16321 aaactacatt gctcattcat tgtgcagcgc ctgttattta gatgatcatt tgttgacttt
    16381 tcgtacttat agcaagcaat tttttgattt attgcttata atacatttta agacaaatgc
    16441 ttgtttcaac atcgcatacg acatcattca ttattcattt gaatttatcc agtttctatt
    16501 tgtctgcaat tgctgaaaat taaaggagct tatttttaat aatggaacaa attatcactg
    16561 aatttattag tcgttttggg tatgcagcca tttttatatt aattttatta gaaaatgttt
    16621 tacctatcgt accatcggaa attattctga catttgctgg ccttatgtct gttaaatcac
    16681 atttatcaat tttaacctta tttattattg caaccatcgc atcgtttata gggctgttaa
    16741 ttttatatta tatctgtcgt ttgatttcag aagaacgtct atatcgtttt attgatcgac
    16801 acggtaagtg gattaaattg aaaagtaagg atttaaagag agcaaatgat tggtttaaaa
    16861 agtatggcgt atgggctgta tttatctgtc gtttcatacc tgtattacgt gtattgatta
    16921 ccattccagc cggtgtaaac cgcatgaatg ttgtgacatt taccgttatt tcattaatag
    16981 gtacaacaat ttggaatttc ggtttaattt tactaggacg cactttgagc gatagctttg
    17041 gtatgttgat gactggtctt catacatatt cacgtattat gtatgttatc atcattatcg
    17101 cagtcattta ctttgctata cgttacatcg gcaaacgtaa aagagtaaaa taatattgtt
    17161 gttagtataa aggcatgaat tttaaatttc tatacacaaa aagcagtaaa atcattccat
    17221 ctattgaaat gattcactgc ttttttaagt ttatttggca aactttagct gaaagtcacc
    17281 tatctttata taaagaaata ttattattaa catgatagtt acagttgtga aataaaatgg
    17341 taccaatgag tgagaaatga taccaattaa taatgcgccg acagtcattc caatatatct
    17401 cacaaagtta aatacaccta acacttttcc ttttatatgt tcaaaccttt tagaaattat
    17461 agtagacagc aaagctggca caataccaac acaagtacct aatataaata taattactga
    17521 taatataatt acatttgatt cgtttaacca tccaaacaat aatgtaaata tagcaaatcc
    17581 agttatagta acacgaagta tcattatttc attatatttt tttgacaatc gtttataaaa
    17641 taaacttcct aatataacac taacagtaat aggtaaaaac aaaactcctg atattccaac
    17701 gcctatatca taggtattat ttaataatgt cggtaaataa actaatattg aaaagtacgt
    17761 caaaaatatt aaaaatccca atgtcactaa agtaataaac actttatcga tcaaatattt
    17821 ttcttcaatt ttttcagtta attttacaat ccctttttca acatttggaa tatcaataaa
    17881 aaagataatc agaaataata cgatagatat aacaagcaaa aaactaaaaa taccaatata
    17941 atcccatctt gcacctaaag tactccctag gattggtgcc aaagctggcg cacatgataa
    18001 aaatatttga tagttcgcca tagcttgagc tctaccatta tctgtactta gtttggctaa
    18061 taaagttaaa gtcacgagag gaataattcc gcaaccaatt gcttgaaata accttgatat
    18121 agcaaataat ataaagttat gtgtcattgc acaaataaag ctagatatga ttactattcc
    18181 caatcccgtt aaaagtactt ttttcgaatc acgagaatct atacttctac caagtacaat
    18241 ttgcataact gcaacgataa atataaaacc gccaactgtc gcatttatcc aaaatagtgg
    18301 tacttgaaaa tcgtcatata gtctcggaat aataggcgtg tatatgtttt gaatcaatga
    18361 tgcgaaaaat gctaaaaatc ctagaatata taatctgata ttactattag tcatttaaac
    18421 catcttcttc tttttttaat tcttctatac tacggtttaa aaactctaca ataactgcca
    18481 tttcatcatt atcaaagact ttaagtactt cttcatattt ttttaccgct ttttcatgta
    18541 attcatcatg tatataagat aatttttttc cttct
//
LOCUS       DAHHOJ010000051        17580 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.051, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000051 DAHHOJ010000000
VERSION     DAHHOJ010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17580
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.051"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..232)
                     /locus_tag="NBH32_001483"
     CDS             complement(<1..232)
                     /locus_tag="NBH32_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000364405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515083.1"
                     /translation="MDLKKFEELYKDYNDDDVIEQFAIYKIKDEKQVMEITDKKLTSE
                     DKQVFQRIANLSYQFNVVKEMYDYYLYDFQVIT"
     gene            complement(304..1887)
                     /locus_tag="NBH32_001484"
     CDS             complement(304..1887)
                     /locus_tag="NBH32_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000271877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515084.1"
                     /translation="MYDFLEYLHRYCVKSINNWEAVADISCKVVREKVSKGYYVKQDS
                     VVIDDNDIPSIIKISGNYVAADILVTRYLDGMGEFIKVSELDQSSYYEWFMFVEETRR
                     IFRGTEIVYRFLKYLFTHTYEHKNEEQKIEKYMKNIIIDNHFRGGHSNDIVRLDMKDD
                     NVLFYNFDIYDTNILYEKIDEEKLLRILDEISNTDNYKALFNIVSENQKKFWKSNIKP
                     IVSECLEDINTIPFDLYVYVNKYISNKIFNCDNYLITKNEVVDFYFNHGSDFNNFKMG
                     QKWTKSKFKQENKLKIHEYIIAVTYVNLLNHSYMKYINAENYYDPSKKITKGMKNNYH
                     KFTDSFSDFVEMVDDITWRIRDSFDYIGGLKNKEFNEDENEYIEQLIQFVILSKFGYM
                     NVENHFDYIENLNTLNNELTTILKSEFAHISNDMYNNLFSNLFDVVNTWKKDYSNNFY
                     NNTQNTITTEKLDIISRFIKQYDKPVDRDKHGKRVKAEVFEGISDFHRRVKEGFDNND
                     VLLQLRLEMSVHENDKFTS"
     gene            2059..2355
                     /locus_tag="NBH32_001485"
     CDS             2059..2355
                     /locus_tag="NBH32_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000273824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HCD1515085.1"
                     /translation="MTYNQKDDFKELKRRILGIVFDLPEGQTFTFHDLNSRANVQCSK
                     AVQQNVGRWFAYFVKHAPRIPFVIIGKNTHGHLVYLKTGPNPYNNSNPSKGGVR"
     gene            2355..4148
                     /locus_tag="NBH32_001486"
     CDS             2355..4148
                     /locus_tag="NBH32_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002469147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HCD1515086.1"
                     /translation="MTQTNPSFNPSPRYKSKKGWYKDKPPKEKGGMPTEVEIAGPIVI
                     ENKFIDPKTNTEKVIITDEDQKEIVESSDILTTQKLPSLMKYGFSINEKYTKDLGYAL
                     QQMRNQLPISYLYEGVGILETPFGPIVSLSEIYTTKEFDNKSPSDAICDNAYDLTPKG
                     TFDNWFNMYLKEVKGSLLLELAVVFGISALVTSFLKHKHETEFAGIIFSFTGQSSTGK
                     STAASLAVSVAGNPTKGNETLFRNWNATRNALEGYLSNNFGIPIVFDELSSATFRDTT
                     GLLYSITEGQGRQRSNVHGEVKTPKNWGTSVISTSEHSIFNDSAQNDGLRVRTIEINE
                     QFTTNATNADNIKKAVALNYGHVLPLVAKYLINREDEVIQWFYKEVDWFEAKLKDDKS
                     NTGNRMFKRYAVITTSAKILGRVLATDIDIAKIRDYFIDYHGHTISERSLADKAIDVI
                     IQFVAQNRGKFSDEGALKNMFENYGLISLKDDHIEVKIIANVFKQMLNNHQFQDVNNV
                     VNALRDKGFILADRGRQTTKRSVKDNSGKKQSLVFYHLKLDVEFASILGLTKDKSLLQ
                     NWTPSNDNKAAKELFKSANEGIGPSGVHEDF"
     gene            4382..5731
                     /locus_tag="NBH32_001487"
     CDS             4382..5731
                     /locus_tag="NBH32_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1515087.1"
                     /translation="MKQVIGYLRQSTMKQQSLAAQKQAIEAIAEKHHIQHINFYSDKQ
                     SGRKDNRSGYRQITQLIQQGQCDILCCYRLNRLHRNLKNALKLIKLCQTYHVHILSVH
                     DGYFDMDQAFDRLKLNIFISLAELESDNIGEQVRNGLQEKAKQGRLITTHAPFGYDYH
                     HGTFIINQNESPTVKAVFNYYIKGHGYKKIAQLLEEDNTYINRQPYQVRNIIINPNYC
                     GRVNNQYGQFDNMFPSIVSTSIYEQAQRLRSQKQTKQTSSDNQLKQKIKCPCCNATLT
                     NMTIRKKNHTLRYYVCPKNMNASRFVCDFKGINAQTLEDKVLEVCRDFYQNQRIYTKI
                     KSAIDKRIKRQRNIEKHHTLTQEQLIEKLAQGIIDAETFREQTQSLRQQPQRTTSING
                     HQIQHIIQNIIQKRFTLNILYPYIETIHITKDKNLIGIYFKNEPLNIVNQTMQSSIA"
     gene            5753..7381
                     /locus_tag="NBH32_001488"
     CDS             5753..7381
                     /locus_tag="NBH32_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001186610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCD1515088.1"
                     /translation="MQQLKTKRVGIYVRVSTEMQSTEGYSIDGQINQIKEYCDFHHFE
                     VKDIYADRGISGKSMNRPELQRMLKDAKEGNIDCVMVYKTNRLARNTSDLLKIVEDLH
                     KQNVEFFSLSERMEVNTSSGKLMLQILASFSEFERNNIVENVFMGQTRRAQEGYYQGN
                     LPLGYDKIPDSKHELMINQHEANIVKYIFECYAKGHGYRKIANALNHKGYVTKKGKPF
                     SISSITYILANPFYIGKIQFAKYKDWSEKRRKGLNDKPVIAEGKHSPIINQDLWDKVQ
                     MRKKQVSQKPQVHGKGTNLLTGIIHCPQCGAPMAASNTTNTLKDGTKKRIRYYSCSNF
                     RNKGSKVCSANSVRADVIEDYVMKQILEIVKSDKVIQRVVTHVNQENQVDGAALHHDI
                     AYKQQQYDEVQIKINNLIKTIEDNPDLTSVIRPSIQKYEKQLNDITNQINQLKNQQNE
                     DKTLFDAKEISKLLQHIFHDIKHIEKSRLKALYLSVIDRIDIKKDGNHKKQFYVTLKL
                     NNEIIKQLFNNKQLDEVHLSTSSLFLPQTLYLTI"
     gene            7903..8253
                     /locus_tag="NBH32_001489"
     CDS             7903..8253
                     /locus_tag="NBH32_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002502216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515089.1"
                     /translation="MKTITQELKQYITHLFQLSNNEAWECEALEEAAENILPERFIND
                     SPLVHLTLETYTYYNDELHELSIYPFLMYANNQLISVGYLDHFDMDFLYLTDTQNIII
                     DERHLLKQGGQDHE"
     gene            8340..8651
                     /locus_tag="NBH32_001490"
     CDS             8340..8651
                     /locus_tag="NBH32_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002505017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HCD1515090.1"
                     /translation="MKINRYITRGINESIPLDLQILLWHMVEKKDNQPHTDYLHIFKL
                     QEDENILSITHEQEQPTYKLEYHYTNYEKNQNALPKKVYVIREDDVDAFYYVMLLPEE
                     Y"
     gene            8666..9172
                     /locus_tag="NBH32_001491"
     CDS             8666..9172
                     /locus_tag="NBH32_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001092170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HCD1515091.1"
                     /translation="MNTIKSTIHTEAIFSDDEQHRYLLKKTWDEKKAACTVITMYPHL
                     DGVLSLDLTTVLILNQLANSERYGAVYLVNLFSNIKTPENLKHIKEPYDEHTDIHLMK
                     AISESDTVILAYGAYAKRPVVIDRVEQVMEMLKPHKKKVKKLINPVTNEIMHPLNPKA
                     RQKWILKS"
     gene            9224..10831
                     /locus_tag="NBH32_001492"
                     /pseudo
     CDS             9224..10831
                     /locus_tag="NBH32_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
     gene            complement(10822..11082)
                     /locus_tag="NBH32_001493"
     CDS             complement(10822..11082)
                     /locus_tag="NBH32_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD1515092.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(11054..12040)
                     /locus_tag="NBH32_001494"
     CDS             complement(11054..12040)
                     /locus_tag="NBH32_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HCD1515093.1"
                     /translation="MLSSFLMLSIISSLLTIYVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            12137..14146
                     /gene="mecA"
                     /locus_tag="NBH32_001495"
     CDS             12137..14146
                     /gene="mecA"
                     /locus_tag="NBH32_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCD1515094.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLEKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(14192..14620)
                     /locus_tag="NBH32_001496"
     CDS             complement(14192..14620)
                     /locus_tag="NBH32_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCD1515095.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(14717..15460)
                     /locus_tag="NBH32_001497"
     CDS             complement(14717..15460)
                     /locus_tag="NBH32_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCD1515096.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
     gene            complement(16337..16708)
                     /locus_tag="NBH32_001498"
                     /pseudo
     CDS             complement(16337..16708)
                     /locus_tag="NBH32_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016912861.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase family
                     protein"
     gene            16762..17436
                     /locus_tag="NBH32_001499"
     CDS             16762..17436
                     /locus_tag="NBH32_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001105942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6-like element IS257 family transposase"
                     /protein_id="HCD1515097.1"
                     /translation="MNYFRYKQFNKDVITVAVGYYLRYTLSYRDISEILRERGVNVHH
                     STVYRWVQEYAPILYQIWKKKHKKAYYKWRIDETYIKIKGKWSYLYRAIDAEGHTLDI
                     WLRKQRDNHSAYAFIKRLIKQFGKPQKVITDQAPSTKVAMAKVIKAFKLKPDCHCTSK
                     YLNNLIEQDHRHIKVRKTRYQSINTAKNTLKGIECIYALYKKNRRSLQIYGFSPCHEI
                     SIMLAS"
BASE COUNT         6407 a         2964 c         2608 g         5601 t
ORIGIN      
        1 cagtaattac ttgaaaatca taaagatagt aatcatacat ttcctttact acattaaatt
       61 gataacttaa attagctatt ctctgaaaaa cttgtttatc ttcactggtt agctttttat
      121 cagtaatttc cattacttgc ttttcatctt ttattttata tatggcaaat tgttctatta
      181 catcatcatc attataatct ttatatagtt cttcaaattt ttttaagtcc ataacttacc
      241 ccttttttat atttaaatat ttatattgct tttgaattag attttacaaa aaatccccat
      301 tacttaacta gtaaatttat cattttcatg tacgctcatt tctaatctta attgtaatag
      361 cacatcatta ttatcaaatc cttcttttac acgcctatga aaatcactta taccttcaaa
      421 cacttctgct ttaactcgtt taccatgttt gtctcggtca actggtttat catattgttt
      481 tataaatcga gatattatgt ctaacttttc tgtagttata gtattctgag tattattata
      541 aaagttattt gaataatcct ttttccatgt atttacaaca tcaaacaaat tactgaataa
      601 attattatac atatcatttg aaatgtgcgc gaactcactt tttaatatag tcgttaattc
      661 attattaagc gtatttaagt tctcaatata atcaaagtga ttttctacat tcatatagcc
      721 gaatttagat aatataacaa attgaattaa ttgttcgata tattcattct catcttcgtt
      781 aaattctttg ttttttaatc ctccaatata atcaaatgaa tcacggattc tccatgttat
      841 atcgtccacc atttctacaa aatcactgaa tgaatctgta aatttatgat aattattttt
      901 cattcctttt gttatttttt tgctagggtc atagtaattt tcagcattga tatatttcat
      961 atatgaatga tttaataaat tcacataagt tactgcaata atatattcgt gtattttaag
     1021 tttattttcc tgcttaaatt tagactttgt ccatttctgt cccatcttga aattattaaa
     1081 gtcactacca tgattaaaat aaaaatccac aacctcattt ttagtaatta aataattatc
     1141 acagttaaat attttattac ttatatactt atttacataa acatataaat caaatggtat
     1201 cgtatttata tcttctaaac attcacttac tattggctta atatttgatt tccaaaattt
     1261 cttttggttc tcacttacaa tgttaaatag cgctttgtaa ttgtcagtat tacttatttc
     1321 atctaaaatt ctaagtaatt tttcctcgtc tattttttca taaagaatat ttgtatcgta
     1381 tatatcaaaa ttataaaata acacattatc atctttcata tctaaacgaa ctatatcatt
     1441 actatgcccc cctctaaaat gattatctat gataatattc ttcatatatt tttctatttt
     1501 ttgttcttcg tttttatgtt cataggtatg cgtaaataga tattttaaaa atcgataaac
     1561 tatttctgta cctctaaata ttcttctagt ttcttcaaca aacataaacc attcgtaata
     1621 gctcgactga tctaattctg atacctttat aaattcaccc attccatcta aatatctagt
     1681 aactaatata tctgctgcta cataatttcc acttatttta ataattgaag gtatatcatt
     1741 atcatcaatt acaacgctat cttgtttaac ataataacct ttactaactt tttcacgtac
     1801 aactttgcat gatatatctg caacagcctc ccaattatta atcgatttta cacaataacg
     1861 atgtaaatat tctaaaaaat catacacttt ggttacctct tttctaattt gtcaagcgaa
     1921 aagtcttgca attatatgtt cgtcttttgc tcaatactat tcgagcaata aagacagtac
     1981 atctctatta taagcaatgt aatcttctga aaaaaaccag aagatagact taattaataa
     2041 tatttgggag gaactcatat gacttacaat caaaaagatg attttaaaga gttgaaaaga
     2101 agaattttag gaattgtgtt cgatttacct gaggggcaaa cgtttacatt tcatgatctg
     2161 aacagtcgtg caaatgtaca atgctcaaaa gctgtacaac aaaatgtcgg acgttggttc
     2221 gcgtactttg taaaacatgc gccacgtatt ccatttgtta ttattggtaa aaatactcat
     2281 ggacacttgg tttatcttaa aacagggcca aatccatata ataacagcaa cccttcgaaa
     2341 ggaggtgttc gctaatgact caaactaacc cttcatttaa tccttcacca cgatacaaat
     2401 caaaaaaagg ttggtataaa gacaagcctc ccaaagaaaa aggaggaatg cctactgaag
     2461 ttgaaatcgc aggtcctatc gttatcgaaa ataagtttat tgaccctaaa actaacactg
     2521 aaaaagtaat tattacagac gaggatcaaa aagaaattgt tgaatctagt gatattttaa
     2581 caacacaaaa actgccatct ttaatgaagt atggatttag tattaatgaa aaatatacaa
     2641 aagatttagg ttatgcgttg caacaaatgc gcaatcaatt acccatttcc tacttatatg
     2701 aaggggtagg tatactggaa acaccgtttg gtcctattgt atcgttaagt gaaatctata
     2761 ctactaaaga atttgataat aaatctccat ctgacgccat ttgtgataat gcttatgact
     2821 taactcctaa aggtacattt gataattggt ttaacatgta tctaaaagag gttaaaggtt
     2881 ctcttctttt agaattagca gtcgtatttg gtatttctgc cttagtgacg agtttcctga
     2941 agcacaaaca tgaaaccgaa tttgccggaa tcatttttag ttttacaggt caatcctcaa
     3001 ccggtaagtc aactgcagct tctttagctg tatcagtcgc tggtaaccct accaaaggta
     3061 atgaaactct ctttagaaat tggaatgcca cacgtaatgc acttgaaggt tacttgagta
     3121 acaattttgg tatacctatt gtatttgatg aactctcatc agcaactttt agagatacaa
     3181 cggggttatt gtactccatc actgaaggtc aaggtcgtca acgttcaaac gttcatggag
     3241 aagttaaaac gcctaagaat tggggtactt ctgtcatttc aacgtcagaa cacagtattt
     3301 ttaatgattc agctcaaaac gatggattac gtgtaagaac catcgagatt aatgaacaat
     3361 tcactacaaa tgcgaccaat gctgacaata ttaaaaaagc tgtagcacta aactatggtc
     3421 atgttttacc gttagtagct aaatatctta taaatcgtga agatgaagtt attcaatggt
     3481 tttacaagga agtagattgg tttgaagcta aattgaaaga tgataaaagc aacacaggca
     3541 atcgtatgtt taagcgttat gcagtcatta caacatctgc aaaaatatta ggtcgtgttt
     3601 tagctacaga tattgatatc gctaaaataa gagactactt tattgattat catggtcata
     3661 caatctctga acgctcatta gctgataaag ctatcgatgt catcattcaa tttgtagcac
     3721 aaaacagagg taagttttca gatgaaggtg ctttgaaaaa tatgtttgag aactatggat
     3781 taatctcatt aaaagatgac catatcgaag taaaaataat tgcgaacgtt tttaaacaga
     3841 tgctaaacaa tcatcaattt caagatgtta ataatgttgt caacgcctta agagataaag
     3901 gtttcatatt agctgaccgt ggacgccaaa caactaaaag aagtgtcaaa gataatagcg
     3961 gtaaaaaaca atcacttgta ttttaccatc taaaattaga tgtagagttt gcatctatcc
     4021 tcggattaac taaagataag tcgttacttc aaaattggac accgtcaaat gataacaaag
     4081 ctgcgaaaga actttttaaa tccgctaatg aagggatagg cccttcagga gttcacgaag
     4141 atttctaatt gtctttatca tacaactgtg aagcatcaaa ctgaataatc gacgtattga
     4201 agatagaaca aataacggag tgaaaaacat ggatgaactt atcgagatat tagccgattt
     4261 ggtaattgaa gaaatcaatc aaacaaatga ctaatatgga cgcccaacat ctcattgatg
     4321 ttgggcttat atatcaattc atccataaat atatattatc actgtgaaag gaggagccga
     4381 tatgaaacaa gtcataggct atttacgtca aagtacgatg aaacaacaat ctcttgcagc
     4441 acagaaacaa gctatcgaag caatagccga aaaacatcat attcaacata tcaactttta
     4501 tagcgacaaa caatcaggtc gcaaagataa tcgtagtggg tatcgacaaa taacacaatt
     4561 aattcaacaa ggacagtgtg acatattatg ctgttatcgt cttaataggt tgcatcgtaa
     4621 tctgaaaaat gcattaaaac tcatcaaatt atgtcaaacg taccatgttc atatcttaag
     4681 cgtacatgat ggttactttg atatggatca agctttcgac cgactcaagc ttaatatctt
     4741 catcagttta gccgaacttg aatcggataa cattggagaa caagtcagaa atgggcttca
     4801 agaaaaagca aagcaaggtc gattgattac aacacatgca ccttttggtt acgattatca
     4861 ccacggaaca ttcatcatca atcaaaatga gtcaccaacg gtaaaggctg tattcaatta
     4921 ttacattaaa ggccatggtt ataagaaaat tgcacagtta ttagaagaag ataacacgta
     4981 tatcaatcga caaccctatc aagttcgtaa cattatcatc aatcctaatt attgtggtcg
     5041 tgtcaacaat caatatggcc aattcgacaa tatgtttcct tctattgttt ccacaagtat
     5101 atatgagcaa gcgcagagac ttcgatcgca aaaacaaacc aaacagacat cttcggataa
     5161 tcaactcaaa caaaaaatca aatgcccatg ttgtaatgca acacttacaa atatgaccat
     5221 tagaaaaaag aatcatacat tacgttacta cgtctgtcct aaaaacatga atgcttcacg
     5281 ctttgtctgt gattttaaag gcatcaatgc acaaacactt gaagataaag tattagaagt
     5341 gtgccgagac ttttatcaaa atcaacgcat ctacacaaaa attaaaagtg cgattgacaa
     5401 acgcatcaaa agacaaagaa acatagaaaa acatcacaca ttgactcaag aacaactgat
     5461 agaaaagttg gcacaaggca tcattgatgc agaaacgttc agagagcaaa cacaatcatt
     5521 acgtcaacaa ccgcaacgca ctacatctat caatgggcat caaatacaac acatcattca
     5581 aaatattatt caaaaacgtt tcacgttaaa catattgtac ccctatattg aaaccattca
     5641 cattacgaaa gataaaaatc ttataggaat ctatttcaaa aatgaaccac tcaatattgt
     5701 caatcaaacc atgcaatcat cgattgcata aatgaagaaa ggatgtaaca taatgcaaca
     5761 acttaaaaca aaacgtgtcg gtatctatgt acgtgtatca acagaaatgc aaagcacaga
     5821 aggttatagt atcgacggac aaatcaatca aatcaaagaa tactgtgact tccatcattt
     5881 tgaagttaaa gatatatacg ctgaccgtgg tatttcaggt aaatctatga acagacctga
     5941 gctccaacgt atgttgaaag atgcaaaaga aggcaatatc gactgtgtta tggtatacaa
     6001 aacaaaccga ttagctcgta atacatctga tcttctcaaa attgtcgaag atttacacaa
     6061 acaaaatgtc gaatttttca gtttgtcaga gcgtatggaa gtcaatactt cttctggtaa
     6121 actcatgtta cagatacttg cgagtttctc agaattcgaa cgtaataaca ttgtcgagaa
     6181 cgtatttatg ggtcaaacga gacgtgccca agaaggctat tatcaaggca atttaccact
     6241 aggttatgac aaaataccag atagtaaaca cgagctaatg attaaccaac atgaagctaa
     6301 tattgtaaaa tatatattcg agtgctatgc caaaggacat ggctatcgta aaattgccaa
     6361 tgcattgaat cacaaaggat atgtcactaa aaaggggaaa cctttcagta ttagttcaat
     6421 cacatacatc ttagctaacc ctttctatat cggcaaaatt caatttgcga aatacaaaga
     6481 ttggagtgaa aaacgtcgta aagggctgaa tgataaacca gtgatagctg aaggtaagca
     6541 ttcccccatt attaatcaag atttatggga taaagtacaa atgcgtaaga aacaagtcag
     6601 tcaaaaaccc caagtccatg gcaaaggaac gaatctgctt acaggcatta ttcactgtcc
     6661 ccaatgtggc gcacctatgg cagcaagcaa taccacgaat actcttaaag acgggactaa
     6721 gaaacgtatt cgttactatt catgtagtaa ttttcggaac aagggttcca aagtatgttc
     6781 ggcaaacagt gtaagagctg atgtgattga agattatgtg atgaagcaaa tacttgaaat
     6841 agtcaaaagt gataaagtca ttcaacgcgt agtaacacac gtcaatcaag aaaatcaagt
     6901 tgatggcgct gcacttcatc acgatattgc ttataagcaa caacaatatg atgaagtaca
     6961 aatcaaaata aataacttga ttaaaaccat cgaggataat ccggacttaa catcagtaat
     7021 cagaccaagt attcaaaaat atgaaaagca actcaatgac attacgaatc aaatcaacca
     7081 actcaaaaat caacaaaatg aagataagac tttatttgat gccaaagaaa tcagtaaact
     7141 attacaacac atctttcatg atattaaaca catagaaaaa tctcgactca aagcattgta
     7201 tctatcagtg attgatcgca ttgatattaa aaaagatggt aatcataaaa aacaattcta
     7261 tgtcacactc aaacttaata acgaaatcat taaacaactt ttcaataata aacaactcga
     7321 cgaagtgcat ctcagcactt cgtctttatt tttgccccaa acactatatc ttactattta
     7381 gctaatagaa tcattgttat aacaataatt ttcactatat tctttcgctt attttatcta
     7441 actattttca agaattgctc tttatcaatt tcaataacac ttatctaatg aaacagttat
     7501 tgtcgtaatt ttttcataac tatacaatgc ttatattgaa attgctaaag aacatcccac
     7561 aatgattttt tatcgggaaa tgattgggat ttgcagattc gaagaaacag aaggattgtg
     7621 caagcacaat cccaaaatcc cgttactttt tataaataaa taatatatat aaggaagcag
     7681 agacacaacc ctatcatcaa tacttataaa taatgagttg attgtttgtc aaaataggcg
     7741 gaatttcagg atttccttgg cataccaacg tttgtagcgg gtttttatat ggaattttct
     7801 cttattccca ccgggattcg taataaatga caaatcaaat atatattatc tcaatgatgt
     7861 atatgaacta catcactatt acactatcta ggaggcaata atatgaaaac catcactcaa
     7921 gaactcaaac aatatataac ccacctattc caactatcta ataacgaagc ttgggaatgt
     7981 gaagcgctgg aggaagcagc agaaaatatt ctaccggaac gttttattaa tgattctcca
     8041 ctggtacatc tcacactcga gacttatacc tactataatg atgaactaca tgagctaagt
     8101 atctatccat ttctaatgta tgccaataac caactcatca gtgtcggata cttagaccat
     8161 tttgacatgg actttctgta ccttacagat actcaaaata tcattattga tgaacgtcat
     8221 ttactaaaac aaggaggtca ggaccatgaa taattggatc atagttgctc aattatccgt
     8281 cacagttatc aacgaaatca tcgacatcat gaaagaaaag cagaagggag ggaaatagca
     8341 tgaaaatcaa tcgatacatc acaagaggaa ttaatgaaag tattccacta gaccttcaaa
     8401 tcttactttg gcacatggta gaaaaaaaag ataaccagcc tcatacagat tacctacaca
     8461 tttttaaatt acaagaagat gagaatatac tctcaatcac acatgaacaa gaacaaccca
     8521 catacaaatt ggaatatcac tatacaaact atgaaaaaaa tcaaaatgca ttacctaaga
     8581 aagtctacgt cattcgtgaa gatgacgtgg acgcttttta ttatgtgatg cttttaccag
     8641 aagaatacta aaggaagtga cgatgatgaa tacaatcaaa agtacgatac acacagaagc
     8701 catatttagc gatgatgaac agcaccgcta tttactcaag aaaacatggg atgaaaagaa
     8761 agctgcatgc acagtgataa cgatgtatcc tcatttagac ggtgtattat cactcgatct
     8821 cacaactgtt cttatcctca accaattagc caattcagaa cgatacggtg ctgtatatct
     8881 tgtaaatcta ttctctaata ttaaaactcc agagaatctt aaacatatca aagagccata
     8941 cgatgagcac acagatattc atttgatgaa agcgattagt gaaagtgaca cagtgattct
     9001 tgcttatggt gcctatgcga agcgaccagt tgttatcgac cgtgtcgaac aagtgatgga
     9061 aatgttaaaa cctcataaaa agaaagtaaa aaagctcatc aatccagtaa caaatgaaat
     9121 tatgcatcca ctcaacccta aggcacgtca aaaatggatt ttgaaatcat agcaaataca
     9181 tggaggtcaa tatggaaaca aaacaaatca acatcgtttt actgtaaatg atattgttga
     9241 aacaattcct gacaatgaat tcgacgaatt cagacatcac cgtggtgcaa cttcgtacca
     9301 tcctaaaatg atgttaaaag tgattctata tgcctacaca caatctgtat tctcaggtcg
     9361 taaaatagaa aaaatgctta atgatagcat ccgaatgatg tggctatcac aaaatcaaaa
     9421 accttcttat aaaacaatta atcaatttag agtaaatcca aaagtagatg ctttattaga
     9481 atctttattt attcaatttt acagtcagtg tgtaaaacaa aatcttatag atgataaagc
     9541 tatttttatt gatggtacaa aaattgaagc aaatgccaat cgatatacat ttgtatggaa
     9601 aaagagtatt caaaaccatg aatcaaagat gaatgaggat tctaaagccc tctaccatga
     9661 attggtaacc aataaaatca taccggaaat taaagaagat catgataatg aattaacaaa
     9721 agaagaaata gatttgattg gtagtcactt agataaagaa atcgaagatt taaaccaaca
     9781 tatcaacaat gaaaaatgta ctaaaacaag aaaacaaata cgtctcaaaa gaactaaaat
     9841 caaaaaatac aaaaagcaaa tcaatgatta ttttgagcga aagtatcgat acgaatttca
     9901 aaaatctatt ttaaaggata gaaatagtta ttctaagaca gattatgatg cgacatttat
     9961 gagaatgaaa gaagatcaca tgaaaaatgg acaacttaag ccagggtata atttacaaat
    10021 agcaacaaat tcccaatttg ttttatctta taatgtgtat caaaatccaa cggatactag
    10081 aacgatgatt ccatttttaa attcaattca agagacctac ggtcatttac ctgaatatat
    10141 tgtagctgat gcaggttatg gtagtgaatc aaattataag gcaattatag atgactttaa
    10201 tcgaacgcca ctcataacat atggaatgtt tataaaagat aaaactaaaa aatataaaag
    10261 tgacatcttt aatactcaaa attggaacta tgacgaaatt aatgacgaat tcatttgtcc
    10321 gaataataaa cggctaggtt ttaaaagata tgcctatcgt catgataagt atggttataa
    10381 gcgagacttc aaattatatg aatgtgatga ttgttcagaa tgtcctctga aaaatcaatg
    10441 tatgaacttc aattcaaaaa caaacaaaaa aataatgaag aattataact gggaatattt
    10501 taaatcccaa attaacaaaa agctttcaga accagaaaca aaaaatatct acagtcaaag
    10561 aaaaattgat gtggaacctg tttttggatt tatgaaggct attttgggtt tcactcggat
    10621 gtctgtccga ggactcaata aagtcaaaag agaacttggt tttgtattaa tggcacttaa
    10681 tataagaaaa gtagtagctc aacgagctga aaataatcaa aaaatttata aaaaagacaa
    10741 tttctatatt atttcaatag aaattgtctt ttttcactta tccaagaact ttatgtcccg
    10801 gactcattat gatttatttt tttattgctg acttgccatt aattctgctt gctctacaac
    10861 ttgttccaca gccatttttt gaagatctgg tggatagcca tatttcttta ataggcgtct
    10921 aacttgaact ctcattttag ctttagcact gtctcgttta gaccaatcaa cactcatgtt
    10981 atccttaact gttttagtta actcatgtgc aatggctctt aactctttat cgcccaaagc
    11041 ttctttagct gtttcatgtg aagctcgata tactacatac aatatacgca acaatacgct
    11101 tgtttcgatt aggtttaggc attgaatcat aaagtgcaat atacttaaca cgttctttaa
    11161 tatttgaatt aaaacctagt aaatattgtg ctgccacatt atttatgtgc tgagatttta
    11221 aaatagagca ttttaatatc gattcaccat aacgtatatg ttcatggcga ttcaaaattt
    11281 ttaaaacgtt tctatcacat actttttcac agtcattgtc catcattgtt ttacttatat
    11341 atagtgcagg attaaaccag aatatcattt taaaaacaac ataaagctgg ttgaatatta
    11401 agtcatgact tttcacatgt gatagttcat gtagaataat atattcaatt tctttgtcat
    11461 tcatggtttc gactacgaca gttggtagta caatttggga tttcactaaa ccaaatacca
    11521 tcggattatc aatgtttgaa ctataactaa ttgttatatg ctttttgtag aactgcatct
    11581 tactttgaca tactttaagt cgttcattaa gatatgacga ttccaatgac gaacttttaa
    11641 taacatcaat ttgtcggaat gccttaatca tataaaataa gcacaacaaa ctaccaaata
    11701 cccatatcaa aagaatcata tacgttatat ttgaggtctc aaactgatta acattaattg
    11761 ctaagtcttt cgtaacagat gattgttgac catctaacat atgactaacc gaagaagtcg
    11821 tgtcagatac atttcgattc atcatatctt ttgaaaatgt aaaattcgat attttgtaaa
    11881 atggtattaa tggaattaac gtggagacga gcactaataa ccaaatctta tgtgacataa
    11941 tattttgagt atattttata tagagcattc tcactaaaaa aattacatat atcgtgagca
    12001 atgaactgat tatacttaac attaaaaaag atgataacac cttctacacc tccatatcac
    12061 aaaaattata acattatttt gacataaata ctacatttgt aatatactac aaatgtagtc
    12121 ttatataagg aggatattga tgaaaaagat aaaaattgtt ccacttattt taatagttgt
    12181 agttgtcggg tttggtatat atttttatgc ttcaaaagat aaagaaatta ataatactat
    12241 tgatgcaatt gaagataaaa atttcaaaca agtttataaa gatagcagtt atatttctaa
    12301 aagcgataat ggtgaagtag aaatgactga acgtccgata aaaatatata atagtttagg
    12361 cgttaaagat ataaacattc aggatcgtaa aataaaaaaa gtatctaaaa ataaaaaacg
    12421 agtagatgct caatataaaa ttaaaacaaa ctacggtaac attgatcgca acgttcaatt
    12481 taattttgtt aaagaagatg gtatgtggaa gttagattgg gatcatagcg tcattattcc
    12541 aggaatgcag aaagaccaaa gcatacatat tgaaaattta aaatcagaac gtggtaaaat
    12601 tttagaccga aacaatgtgg aattggccaa tacaggaaca gcatatgaga taggcatcgt
    12661 tccaaagaat gtatctaaaa aagattataa agcaatcgct aaagaactaa gtatttctga
    12721 agactatatc aaacaacaaa tggatcaaaa ttgggtacaa gatgatacct tcgttccact
    12781 taaaaccgtt aaaaaaatgg atgaatattt aagtgatttc gcaaaaaaat ttcatcttac
    12841 aactaatgaa acagaaagtc gtaactatcc tctagaaaaa gcgacttcac atctattagg
    12901 ttatgttggt cccattaact ctgaagaatt aaaacaaaaa gaatataaag gctataaaga
    12961 tgatgcagtt attggtaaaa agggactcga aaaactttac gataaaaagc tccaacatga
    13021 agatggctat cgtgtcacaa tcgttgacga taatagcaat acaatcgcac atacattaat
    13081 agagaaaaag aaaaaagatg gcaaagatat tcaactaact attgatgcta aagttcaaaa
    13141 gagtatttat aacaacatga aaaatgatta tggctcaggt actgctatcc accctcaaac
    13201 aggtgaatta ttagcacttg taagcacacc ttcatatgac gtctatccat ttatgtatgg
    13261 catgagtaac gaagaatata ataaattaac cgaagataaa aaagaacctc tgctcaacaa
    13321 gttccagatt acaacttcac caggttcaac tcaaaaaata ttaacagcaa tgattgggtt
    13381 aaataacaaa acattagacg ataaaacaag ttataaaatc gatggtaaag gttggcaaaa
    13441 agataaatct tggggtggtt acaacgttac aagatatgaa gtggtaaatg gtaatatcga
    13501 cttaaaacaa gcaatagaat catcagataa cattttcttt gctagagtag cactcgaatt
    13561 aggcagtaag aaatttgaaa aaggcatgaa aaaactaggt gttggtgaag atataccaag
    13621 tgattatcca ttttataatg ctcaaatttc aaacaaaaat ttagataatg aaatattatt
    13681 agctgattca ggttacggac aaggtgaaat actgattaac ccagtacaga tcctttcaat
    13741 ctatagcgca ttagaaaata atggcaatat taacgcacct cacttattaa aagacacgaa
    13801 aaacaaagtt tggaagaaaa atattatttc caaagaaaat atcaatctat taactgatgg
    13861 tatgcaacaa gtcgtaaata aaacacataa agaagatatt tatagatctt atgcaaactt
    13921 aattggcaaa tccggtactg cagaactcaa aatgaaacaa ggagaaactg gcagacaaat
    13981 tgggtggttt atatcatatg ataaagataa tccaaacatg atgatggcta ttaatgttaa
    14041 agatgtacaa gataaaggaa tggctagcta caatgccaaa atctcaggta aagtgtatga
    14101 tgagctatat gagaacggta ataaaaaata cgatatagat gaataacaaa acagtgaagc
    14161 aatccgtaac gatggttgct tcactgtttt attatgaatt attaataagt gctgttactt
    14221 ctcccttaaa tacaatttct tcattttcat tgtatgttga aagtgacact gtaacgagtc
    14281 cattttcttt ttttatggat ttcttatttg taatttcagc gataacgtac aatgtattac
    14341 ctgggtatac aggtttaata aatttaacgt tattcatttg tgttcctgct acaacttctt
    14401 ctccgtattt accttcttct acccataatt taaatgatat tgaaagtgta tgcatgccag
    14461 atgcaatgat acctttaaat ctactttgtt ctgctttttc tttatctata tgcatatatt
    14521 gaggatcaaa agttgttgca aattggataa tttcttcttc tgtaatatga aggctttttg
    14581 ttttgaatgt ttctcctact ataaaatcat cgtatttcat atatgtctct ctttcttatt
    14641 caaattaatt ttttagtatg taacatgtta aaggtaagtc taccgtcact gaaacgtaag
    14701 actcacctct aactttctat tgagacaaat gcaccatttt atctgcattg tctgtaaaga
    14761 taccatcaac tccccaatta gcaagttggt ttgcacgtgc tggtttgttt acagtccata
    14821 cgttcaattc ataacccgct tcttttacca tttttacttt tgctttagta agtttggcat
    14881 cttcagtgtt tactatttta gcattacagt aatctaaaag tgttctccag tcttcacgaa
    14941 acgaagttgt atggaatata actgctctgt tatattgtgg catgatttct tctgcaagtt
    15001 taacaagcac aacattaaag cttgaaatga gcacttcttg attctgattt aagtttgtta
    15061 attgttcttc cacttgctta accatacttt tagaaagtgc tagtccattc ggtccagtaa
    15121 taccttttaa ttctacattt aaattcatat tatattcatt tgctattttt actacatcat
    15181 cgaaagttgg caaatgttca tctttgaatt tttcaccaaa ccaagatcct gcagaagcat
    15241 ctttaatttc atcataattc aattcagtta tttccccgga catatttgta gtccgttcta
    15301 aataatcatc atgaatgata atcagttgtt catcttttgt aattgcaaca tctaactcca
    15361 accagtttat accttctact tctgaagcag ctttaaatga tgcaattgta ttttccggag
    15421 ctttactagg taatcctcta tgtccatata cagttagcat attacctctc cttgcatttt
    15481 tattttttta attaacgtaa ctgtattatc acattaatcg cacttttatt tccattaaaa
    15541 agagatgaat atcataaata aagaagtcga tagattcgta ttgattatgg agttaatcta
    15601 cgtctcatct catttttaaa aaatcattta tgtcccaagc tccattttgt aatcaagtct
    15661 agtttttctg taccccttat ctgcaatttt acttaggatt gcttttaact taccccttat
    15721 cagcaatttt actgagaact gcttttaacg cacctcttat ctgcaatttt gcctagaact
    15781 gcttttaacg tacctcttat ctgcaatttt actgagaact gcttttaact taccccttat
    15841 cagcaatttt gcatggaatt gcttttaacg tacctcttat ctgcaatttt acttagaact
    15901 gcttttaaca aacctcttat ctgcaatttt acttagaact gcttttaacg tacctcttat
    15961 ctgtaatttt actgagaact gcttttaaca aacctcttat ctgcaatttt acttagaact
    16021 gcttttaaca aacctcttat ctgcaatttt acttagaatt gcttttacta ttcctcttat
    16081 tagtataatc tcagtaagaa tgcgtataaa aatgaaaatt acaaccgatt ttgtaagtgc
    16141 tgacgcctga gggaatagta tgtgcgagag actaatggct cgagccatac ccctaggcaa
    16201 gcatgcacgt acaaaatcgt aagataaaaa aataagcata tcactgtaaa ctttaaaaaa
    16261 tcagtttagt gatatgctta tttatttcga gttaggattt atgtcccaag ctcatcaagc
    16321 acaatcggcc actagtttat ttctctatct tatatgttct gatatggtct tctatactgt
    16381 ataagtatac ttttgaatat ggatcttgtg tcaattcacg ttcgaaatca aattcttgat
    16441 tatcaaatct gttaaagaat gtttcgtatt cttcgactga taattgctct ctagattcta
    16501 gcatatttaa gtgtttctct ttatctaatg ctttgtcata tcctttaacg attgaaccac
    16561 taaagatttc tcctactgct cctgaaccat aactaaatag acatactttc tcttctggtt
    16621 ggaatgtgtg gttctgtaat aacgaaatta aacttaagta taatgatcct gtataaatgt
    16681 taccaacatc tctattccat aatacggttc tgttgcaaag ttgaatttat agtataattt
    16741 taacaaaaag gagtcttctg tatgaactat ttcagatata aacaatttaa caaggatgtt
    16801 atcactgtag ccgttggcta ctatctaaga tatacattga gttatcgtga tatatctgaa
    16861 atattaaggg aacgtggtgt aaacgttcat cattcaacgg tctaccgttg ggttcaagaa
    16921 tatgccccaa ttttgtatca aatttggaag aaaaagcata aaaaagctta ttacaaatgg
    16981 cgtattgatg agacgtacat caaaataaaa ggaaaatgga gctatttata tcgtgccatt
    17041 gatgcagagg gacatacatt agatatttgg ttgcgtaagc aacgagataa tcattcagca
    17101 tatgcgttta tcaaacgtct cattaaacaa tttggtaaac ctcaaaaggt aattacagat
    17161 caggcacctt caacgaaggt agcaatggct aaagtaatta aagcttttaa acttaaacct
    17221 gactgtcatt gtacatcgaa atatctgaat aacctcattg agcaagatca ccgtcatatt
    17281 aaagtaagaa agacaaggta tcaaagtatc aatacagcaa agaatacttt aaaaggtatt
    17341 gaatgtattt acgctctata taaaaagaac cgcaggtctc ttcagatcta cggattttcg
    17401 ccatgccacg aaattagcat catgctagca agttaagcga acactgacat gataaattag
    17461 tggttagcta tattttttta ctttgcaaca gaaccgaaaa taatctcttc aatttatttt
    17521 tatatgaatc ctgtgactca atgattgtaa tatctaaaga tttcagttca tcatagacaa
//
LOCUS       DAHHOJ010000052        17209 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.052, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000052 DAHHOJ010000000
VERSION     DAHHOJ010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17209)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17209)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17209
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.052"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(56..889)
                     /locus_tag="NBH32_001500"
     CDS             complement(56..889)
                     /locus_tag="NBH32_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1515098.1"
                     /translation="MEVKTFYNGNTMPKIGLGTFRVENDENCMESVKYAIEQGYRSID
                     TAKVYGNEEQVGAGIRAGLESTGIAREDLFITSKLYFEDFGRENVAAAYEASLSRLGL
                     KYLDLYLVHWPGTNEAVMVDTWKGMEDLYKNNKVKNIGVSNFEPEHLEALLAQVSIKP
                     VINQVEYHPYLTQHKLKLYLAAQHIVMESWSPLMNAQILNDEAIKDIAQELGKSPAQV
                     VLRWNVQHGVVTIPKSVTPNRISENFQIFDFELSDEQMTRIDGLNQDKRIGPDPKTFE
                     G"
     gene            complement(1106..2014)
                     /locus_tag="NBH32_001501"
     CDS             complement(1106..2014)
                     /locus_tag="NBH32_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NERD domain-containing protein"
                     /protein_id="HCD1515099.1"
                     /translation="MDLSSPIVIGLIIAVVIALIFFVLFLVALGSKKKVKRQTEEKYE
                     QQEQNIKKSHEEALEKERIQNKKTITKQQEDYNHMVSTKDREIDALKLFSKNHSEYVT
                     DMRLIGIRERLVKEKRIRPEDMHIMANIFLPKDGFNNIERISHIVLTRTGLYIIDSQL
                     LKGHVYNGISGGQFKDLPPMEQVFDTLDLDKSRPQTIVMDQNDDKRSLSFVNYSDQIE
                     AIKQLAEDLQKELGAKYTPTSILYFNPKNEGDVTISNYNQNSAVKVLVGAEQLDEFFN
                     KFVFHGRIQYNVEDLQQMMDKIESFN"
     gene            complement(2139..3332)
                     /locus_tag="NBH32_001502"
     CDS             complement(2139..3332)
                     /locus_tag="NBH32_001502"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCD1515100.1"
                     /translation="MLNNKRLFTSESVTEGHPDKIADQVSDAILDAILKDDPNARVAC
                     ETTVTTGMALIAGEISTTTYVDIPKVVRETIKEIGYTRAKYGYDYETMAILTAIDEQS
                     PDIAQGVDKALEYRDKDSEEEIEATGAGDQGLMFGYATNETETYMPLAIYLSHQLAKR
                     LSDVRKDGTLNYLRPDGKVQVTVEYDENDNPVRIDTIVVSTQHAEDVTLEQIQEDIKA
                     HVIYQTVPENLINEQTKFYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGCF
                     SGKDPTKVDRSAAYAARYVAKNIVAAGLADQCEVQLAYAIGVAEPVSIAIDTFGTGKV
                     SEGQLVEAVRKHFDLRPAGIIKMLDLKQPIYKQTAAYGHFGRTDVLFPWEKLDKVEEL
                     KDAVK"
     regulatory      complement(3438..3548)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            3703..5295
                     /gene="pckA"
                     /locus_tag="NBH32_001503"
     CDS             3703..5295
                     /gene="pckA"
                     /locus_tag="NBH32_001503"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCD1515101.1"
                     /translation="MSVDTYTETTKIDKLLKKPTSHFQLSTTQLYNKILDNNEGVLTE
                     LGAVNASTGKYTGRSPKDKFFVSEPSYRDNIDWGDINQPIDEETFLKLYHKVLDYLDK
                     KDELYVFKGYAGSDKDTMLKLTVINELAWHNLFAKNMFIRPESKEEATKIKPNFTIVS
                     APHFKADPEVDGTKSETFVIISFKHKVILIGGTEYAGEMKKGIFSVMNYLLPMQDIMS
                     MHCSANVGEKGDVALFFGLSGTGKTTLSADPHRKLIGDDEHGWNKNGVFNIEGGCYAK
                     AINLSKEKEPQIFDAIKYGAILENTVVAEDGSVDFEDNRYTENTRAAYPINHIDNIVV
                     PSKAAHPNTIIFLTADAFGVIPPISKLNKDQAMYHFLSGFTSKLAGTERGVTEPEPSF
                     STCFGAPFFPLHPTVYADLLGELIDLHDVDVYLVNTGWTGGKYGVGRRISLHYTRQMV
                     NQAISGKLKNAEYTKDSTFGLSIPVEIEDVPKTILNPINAWSDKEKYKAQAEDLIQRF
                     EKNFEKFGEKVEHIAEKGSFNK"
     gene            complement(5673..6419)
                     /locus_tag="NBH32_001504"
     CDS             complement(5673..6419)
                     /locus_tag="NBH32_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HCD1515102.1"
                     /translation="MPIESFTHQLEEITYLSDDLQVKALMMTPHHEVKRIVVYLRGGK
                     GQVGRVRAGRLMQFSDSQTLVIGPYYRGNNGSEGKDEFYRGDLNDVTELLRLLHDKYP
                     QAFIHMVGFSRGGLQGLLTFQDLPVNSYIIWGGVSDIDLMYEERVDLRGMLRRMIGHP
                     KKDRAAYEARQAIPNINENSPPILIVHGGKDQQVGIHHAYYLADQLELKGATHETFYQ
                     MAEGHVPRPPAMVETLTYIKEFMNQVELHS"
     gene            complement(6424..6897)
                     /gene="ytkD"
                     /locus_tag="NBH32_001505"
     CDS             complement(6424..6897)
                     /gene="ytkD"
                     /locus_tag="NBH32_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase YtkD"
                     /protein_id="HCD1515103.1"
                     /translation="MKFRDKDNRQVNLTFKKDNEVADGNHVLAIPTFKNQLLFTKHNL
                     RGIEFPGGKRERGESSAEAVTRELYEETGAKVKNIHYIAQYTIETHDQTDFVKDVYFI
                     EVESLVSKNDYLETAGPVLFNCINDIELTQRSFLLQDSTILKCVERVQSLGFYQT"
     gene            6963..7220
                     /gene="yidD"
                     /locus_tag="NBH32_001506"
     CDS             6963..7220
                     /gene="yidD"
                     /locus_tag="NBH32_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCD1515104.1"
                     /translation="MKKIFLAMIHFYQRFISPLTPPTCRFYPTCSEYTREAIQYHGAF
                     KGLYLGIRRILKCHPLHKGGFDPVPLKKDKSESKHSHKHNH"
     gene            complement(7217..8218)
                     /gene="menC"
                     /locus_tag="NBH32_001507"
     CDS             complement(7217..8218)
                     /gene="menC"
                     /locus_tag="NBH32_001507"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCD1515105.1"
                     /translation="MKLTALHFYKYSEPFKSQIVTPKVTLTHRDCLFIELIDDKGNAY
                     FGECNAFQTDWYDHETIASVKHVIEQWFEDNRNKSFETYEAALKLVDSLENTPAARAT
                     IVMALYQIFHVLPSFSVAYGATVSGLSNKQLESLKATKPARIKLKWTPQIMHQIRVLR
                     ELEFHFQLVIDANESLDRQDFTQLQLLAREQVLYIEEPFKDISMLDDVVDGTIPPIAL
                     DEKATSLLDIINLIELYNVKVVVLKPFRLGGIDKVQTAIDTLKSHGVKVVIGGMYEYG
                     LSRYFTAMLARKGDYPGDVTPAGYYFEQDVVAHSGILKEGRLEFRPPLVDITQLQPY"
     gene            complement(8223..9701)
                     /gene="menE"
                     /locus_tag="NBH32_001508"
     CDS             complement(8223..9701)
                     /gene="menE"
                     /locus_tag="NBH32_001508"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCD1515106.1"
                     /translation="MDFWLYKQAQQNGHHIAITDGQESYTYQNLYCEASLLAKRLKAY
                     QQSRVGLYIDNSIQSIILIHACWLANIEIAMINTRLTPNEMTNQMKSIDVQLIFCTLP
                     LELRGFQIVSLDDIEFAGRDITTNGLLDNTMGIQYDTSNETVVPKESPSNILNTSFNL
                     DDIASIMFTSGTTGPQKAVPQTFRNHYASAIGCKESLGFDRDTNWLSVLPIYHISGLS
                     VLLRAVIEGFTVRIVDKFNAEQILTMIKNERITHISLVPQTLNWLMQQGLHEPYNLQK
                     ILLGGAKLSATMIETALQYNLPIYNSFGMTETCSQFLTATPEMLHARPDTVGMPSANV
                     DVKIKNPNKEGHGELMIKGANVMNGYLYPTDLTGTFENGYFNTGDIAEIDHEGYVMIY
                     DRRKDLIISGGENIYPYQIETVAKQFPGISDAVCVGHPDDTWGQVPKLYFVSESDISK
                     AQLIAYLSQHLAKYKVPKHFEKVDTLPYTSTGKLQRNKLYRG"
     gene            complement(9860..10315)
                     /locus_tag="NBH32_001509"
     CDS             complement(9860..10315)
                     /locus_tag="NBH32_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4909 domain-containing protein"
                     /protein_id="HCD1515107.1"
                     /translation="MKICLMLYLLIFLSSCGTNNKIVGQRFEIQQHNETIGSIYFSAD
                     YAHIRGIEKGTAKYFIDKVGSKRYLFIEYIPDNVLNCKPDFWKTLKYKKDKVTYYVYL
                     IENLDDEVFHLSALQDMNRIPIDIADDVATMGKSPHQNDRMTLKLNKNN"
     gene            10617..11266
                     /locus_tag="NBH32_001510"
                     /pseudo
     CDS             10617..11266
                     /locus_tag="NBH32_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500419.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="excalibur calcium-binding domain-containing
                     protein"
     gene            11347..12342
                     /locus_tag="NBH32_001511"
     CDS             11347..12342
                     /locus_tag="NBH32_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HCD1515108.1"
                     /translation="MSNQFKSEEERRQWEQFQAFQNQQNQQNQQYGQKKSKKGWFWGC
                     GGCLVLFILIIIGISACTAGITGNLGGNSSKETNKTHKIGETVKNGDLEVTVNSVETM
                     KSVGPSLAPTNAKGTFVVADVTIKNKGKEALTIDSSMFKLKSGDKTFEADNTGSMSAN
                     QSDNGSIENSFFLQRINPDSTAQGKIVFDVSENIANAKDKKLEVISSLFSVKKITFDL
                     SEAKKTSKAKKDKQDTEVAVASSNSDNISYEASATTPATTSSADTDSEDSEKSSKDED
                     KQNASKSDKSSVEKSESNEETAPVEPMRQSKPTTSEAPPSQNIHNEDSMYDASTE"
     gene            12418..13044
                     /locus_tag="NBH32_001512"
     CDS             12418..13044
                     /locus_tag="NBH32_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515109.1"
                     /translation="MKFKAIVAITLSLSLLTACGANQHKENNSKSNNTNKKTQQTDNT
                     TKSNTDKQMTPQEAEDIVRNDYKARGANENQTLNYKTNLERSNEHEYYVEHLVRDAVG
                     TPLKRCAIVNRHNGTIINIFDDVSEKDKEEFEAFKKRSPKYNPGMSDQAEMDNESEDI
                     QHHDIDNNKAIQNDIPDQKVDDKSDKNAVNKEEQHDNGTNNSEETKVK"
     gene            13085..13426
                     /locus_tag="NBH32_001513"
     CDS             13085..13426
                     /locus_tag="NBH32_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3969 family protein"
                     /protein_id="HCD1515110.1"
                     /translation="MITFENIQQLEKYTLMTMHGLFNQLKLGIISIDNAEHTLFTPYM
                     MESLSSLGVKPDIIDLIHKGTELEDFAAFNLSIDDTVTVCLQRTEELLKQYKNVEFND
                     KILINWRVIQK"
     gene            13527..14099
                     /locus_tag="NBH32_001514"
     CDS             13527..14099
                     /locus_tag="NBH32_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515111.1"
                     /translation="MEKNEYTAKYNEYNQLLDATYSQAVAYLLNKYGAVTDDYYKEKS
                     YTRFLNGEIKSITKGKYTRASEGLYCHHISEDKFQNLSDLRFISEFKYSYNYQKKENL
                     VYCDLIEHLILHAIITKESDGQFGAAGLCQMIKPTVIDWYISEYNPKPAWMQATKARA
                     YLPRILVEKLLIKIDDMLEGIEIYDFLESR"
     gene            complement(14296..14865)
                     /locus_tag="NBH32_001515"
     CDS             complement(14296..14865)
                     /locus_tag="NBH32_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000864138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HCD1515112.1"
                     /translation="MKVIEKVHYEDILESKIVRLFQAFIKDKMDQLFITSEHFAIDID
                     KLLFQLNLNVKYIKMEGNTSGYLSGRTIYINNNHSINNTRFAIARELGRYLFKNSDLM
                     RDNNDNSLKNLHEMIYESDVNQFSVDLLMPKKQIEALVYNFYEVNNINLSSGLSEKER
                     NKLLNLISTKLEVSKVAAGLRLYNLGIHI"
     gene            complement(15238..15414)
                     /locus_tag="NBH32_001516"
     CDS             complement(15238..15414)
                     /locus_tag="NBH32_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515113.1"
                     /translation="MDRLKYSLKVGILALLLFCTLNYLVPMQSNAFSIIIYSAIFAVL
                     LMLLVYIFLGILKK"
     gene            complement(15425..15808)
                     /locus_tag="NBH32_001517"
     CDS             complement(15425..15808)
                     /locus_tag="NBH32_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515114.1"
                     /translation="MSNQNLFDELEKKGYKLEDIFTKEEIKKFKAEDQLRAGKTQYVE
                     TGKDTATLYLSSAYTKTIAALGAGTISVISALTGGLVGAGVGSFLGSIAASNIDTSKR
                     IYLKLKTKKNAAGEYVLIGEKWGYQ"
     gene            complement(16388..16492)
                     /locus_tag="NBH32_001518"
     CDS             complement(16388..16492)
                     /locus_tag="NBH32_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001807442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1515115.1"
                     /translation="MVRLYENGKPRNEIIREYDLTPLTLGKWIKQHQK"
     gene            16637..16732
                     /gene="pepA1"
                     /locus_tag="NBH32_001519"
     CDS             16637..16732
                     /gene="pepA1"
                     /locus_tag="NBH32_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin
                     PepA1"
                     /protein_id="HCD1515116.1"
                     /translation="MMLIFVHIIAPVISGCAIAFFSYWLSRRNTK"
     gene            16855..16956
                     /locus_tag="NBH32_001520"
     CDS             16855..16956
                     /locus_tag="NBH32_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515117.1"
                     /translation="MQGFKEKHQELKSALCQIGLMRSISEVRQLNIA"
     gene            complement(16934..17106)
                     /locus_tag="NBH32_001521"
                     /pseudo
     CDS             complement(16934..17106)
                     /locus_tag="NBH32_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066885.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         5761 a         3131 c         2568 g         5749 t
ORIGIN      
        1 atgtcccagc ctgtgacaat tcatctaacc ttttcatcag ttgagcgatt ttaacctagc
       61 cttcaaaagt ttttggatca ggtccaattc tcttatcttg atttaaacca tcaattcgtg
      121 tcatttgttc atctgataat tcgaaatcaa atatttggaa gttttcagag attctgtttg
      181 gtgttaccga tttagggatt gtaaccacac catgctgcac attccatctt aaaacaactt
      241 gggcaggtga ctttcctaat tcttgagcaa tgtctttaat tgcctcatca tttaaaattt
      301 gtgcattcat caatggtgac caagattcca tcacgatatg ttgtgctgcc aaatataatt
      361 tcaatttatg ttgcgttaaa tatggatgat attcaacctg attaattaca ggtttaattg
      421 acacttgtgc caacaaagct tccaaatgtt caggttcaaa attgctgaca cctatatttt
      481 taactttatt atttttatat aaatcttcca tacctttcca tgtatcaacc attacggctt
      541 cgttcgtacc tggccaatgt actagataca aatctaagta ttttaaacct aatctagata
      601 aactagcttc gtaagcagct gctacatttt cacgaccaaa atcctcaaaa tataattttg
      661 aagtaataaa taagtcttct ctagcaattc cagttgattc taatccggca cgaatgccag
      721 cacctacttg ttcttcattc ccataaactt ttgcggtatc aatactacga tatccttgtt
      781 caatggcata cttaacactt tccatgcaat tttcatcatt ttccacacga aatgtcccta
      841 aaccaatttt tggcatcgtg tttccattat aaaatgtttt aacctccata aatatcgcct
      901 cacctttttg atgtattata ccctgttatc ataacaaatc tgagttgaat acatgagaaa
      961 aaacacttaa cttatagcaa tcaatcacta aaatgctagt aatatctttc aaatattaat
     1021 caaaatgtaa aagcaattct gtttaattta tgacaaacta aaaaagccga agtaacaaca
     1081 tatagtcatc acttcagcct aacatttaat tgaatgattc aattttatcc atcatttgtt
     1141 gtaagtcttc cacgttgtat tgaatacgac catggaatac aaatttgtta aagaactcgt
     1201 ctaattgttc agcaccgaca agcactttga cagcactatt ttgattataa tttgaaatcg
     1261 ttacatcgcc ttcattttta ggattaaagt ataaaattga agttggcgta tatttggcac
     1321 ctaattcttt ttgtaaatct tcagccaatt gtttaatcgc ctcaatttga tctgaataat
     1381 ttacaaatga taatgaacgt ttgtcatcat tttgatccat cacaatagtt tgcggtctag
     1441 atttatctaa atctaatgta tcaaatactt gttccattgg tggtaaatct ttaaattgac
     1501 cgccacttat accattataa acatgacctt ttaacaattg agaatcaatg atataaagtc
     1561 cagttcttgt taagactata tgactaatac gttcaatatt attaaagcca tccttcggta
     1621 aaaagatatt tgccataatg tgcatatctt ctggtcgaat tcgtttttct ttaactaatc
     1681 tttcacgaat accaattaat ctcatgtccg ttacatattc actatgattt ttcgagaaca
     1741 attttaatgc gtcaatctca cgatcttttg tactaaccat gtgattataa tcttcttgtt
     1801 gttttgtaat tgtcttttta ttttgaatac gctctttctc taaagcttct tcatgagact
     1861 ttttaatgtt ttgttcttgt tgttcatact tttcttctgt ttgtcgctta acttttttct
     1921 tactacctaa ggcaactaag aaaaggacaa aaaagattaa tgcaatgact actgcaataa
     1981 tgagtccaat gactatcggt gaagataaat ccatcacaac aacgctcctt tttaatatat
     2041 gaataacttt aattataata gaaaagctaa agattttcga tacatattat catttatata
     2101 ccgaaaatct tttatttagc tatattcaat tcatcttatt attttactgc gtcttttaat
     2161 tcttccactt tgtctaattt ttcccatggg aataatacat ctgtacgtcc aaaatgacca
     2221 taagcagcag tttgtttgta aattggttgt ttcaaatcaa gcattttaat aataccagca
     2281 ggtcttaggt caaagtgttt tctaactgct tcaacaagtt gcccttcaga aactttacct
     2341 gttccaaatg tatcaattgc aattgacact ggttctgcaa caccaatcgc atatgccaat
     2401 tgtacttcac attgatctgc taaacctgct gcaacaatat ttttagccac ataacgtgca
     2461 gcgtatgcag ctgaacggtc tacttttgta ggatccttac cactgaagca tccgccacca
     2521 tgacgtgcat agccaccgta cgtatcaaca atgattttac gtcctgttaa tcctgcatca
     2581 ccttgaggtc caccgattac aaagcgtcct gtaggattga tgtagaattt agtttgttca
     2641 ttaatcaagt tttctggaac agtttgataa atgacatgag ctttaatgtc ttcttgaatt
     2701 tgttcaagtg tcacatcctc agcatgttgt gttgatacga caatcgtatc aatacgtact
     2761 gggttatcat tttcatcata ttcaacagtg acctgaactt taccgtctgg tcgtaaataa
     2821 tttaatgtac catctttacg cacatctgat aaacgttttg ccaattgatg tgataaataa
     2881 attgctagag gcatatacgt ttctgtttca ttcgttgcgt aaccaaacat taaaccttgg
     2941 tctcctgcac ctgttgcttc aatttcttct tcgctatctt tatcacgata ctctaatgct
     3001 ttgtccacgc cttgtgcaat gtcaggtgat tgttcatcaa tcgcagttaa aattgccatt
     3061 gtttcataat catagccata ttttgctctt gtgtatccaa tttctttgat tgtttctcta
     3121 acaactttcg gaatatcaac atatgttgtt gtagaaattt cgccggcgat caatgccata
     3181 cctgttgtaa cagttgtttc acaggctaca cgtgcatttg ggtcgtcttt taaaatagca
     3241 tctaatattg catctgacac ttggtcagcg attttatctg ggtgtccttc tgtaacagac
     3301 tctgaagtaa ataatcgttt gttatttaac atagtttgct cctttaaatt tatattacga
     3361 aaattctctc tctgtgagct aaataaaaaa gaccttctaa ctattaatat agagagaagg
     3421 cctaatacgt ccattcgctc ttatcgttca gacctatttg tctgcaaacg gtttggcacc
     3481 tttcttttat aaaaaagagg ttgctgggtt tcattgggtc catgtccctc caccactcag
     3541 gataagagaa tccgttaaaa ataatagtac ctaattaatg aattaatgtc aatttttcat
     3601 aaataaattt ccagtaaaat attgtagatt aattatgtta atgtgttata ctaattaaat
     3661 gtaaaggctt acatttaaat tatcgctttg gagggattta ggatgtcagt agacacatac
     3721 actgaaacaa ctaaaattga caaattactg aaaaaaccaa cgtcacattt tcaactttcg
     3781 acgacacaac tttataataa aattttagac aacaatgaag gtgtattaac agaacttggt
     3841 gctgttaatg ctagcactgg taaatatact ggtcgttcac ctaaagacaa attttttgtc
     3901 tcagaacctt catatcgaga taacatcgat tggggagata ttaatcaacc gattgatgaa
     3961 gaaactttct tgaagttata ccacaaagta ttagactatt tagataaaaa agatgaatta
     4021 tacgtattta aaggctacgc tggtagcgat aaagatacaa tgttaaaact tacagtcatc
     4081 aatgaattag catggcataa tttatttgct aaaaatatgt ttattagacc tgaatcaaaa
     4141 gaagaagcta caaagattaa acctaacttc actatcgttt ctgccccaca ttttaaagca
     4201 gatccagaag ttgatggtac taaatctgaa acctttgtca ttatttcatt taaacacaaa
     4261 gtcattttaa tcggcggtac tgaatacgct ggtgaaatga aaaaaggtat cttctctgta
     4321 atgaattatc tcttaccgat gcaagatatt atgagcatgc attgctcagc aaacgttggt
     4381 gaaaaaggcg atgttgcatt attctttggt ctatctggca ctggtaaaac aaccctatca
     4441 gcagatccac accgtaaact aatcggtgat gatgaacacg gctggaataa aaacggggtc
     4501 tttaatatcg aaggtggctg ctatgcaaaa gcaattaatc tttccaaaga aaaagaacca
     4561 cagatttttg acgcaatcaa atatggtgca attttagaga acactgtagt tgcagaagat
     4621 ggttcagtgg actttgaaga caatcgttat acagaaaaca cgcgtgccgc ttatccaatt
     4681 aatcacattg acaatattgt agtaccatca aaagcagcac atccaaatac aattattttc
     4741 ttaactgcgg atgcatttgg tgttattcca ccgatttcaa agttaaataa agaccaagca
     4801 atgtatcatt tcttgagtgg tttcacttct aaattagctg gtacagagcg tggtgtgaca
     4861 gaacctgaac catcattctc aacatgtttc ggcgcaccgt tcttcccgtt acaccctact
     4921 gtttacgctg atctattagg tgaacttatc gatttacatg atgttgatgt ttatcttgtt
     4981 aatactggat ggactggcgg aaaatatggt gtaggacgta gaatcagctt acattacaca
     5041 cgtcaaatgg taaaccaagc gatttctggc aaattgaaaa atgcagaata tacaaaagat
     5101 agtacgtttg gtttaagcat tcctgtagaa attgaagatg taccgaaaac tattttaaat
     5161 ccaattaatg catggagcga caaagagaaa tataaagcac aagcagaaga tttaattcaa
     5221 cgttttgaaa agaacttcga aaaatttggt gaaaaagttg aacatattgc tgaaaaaggt
     5281 agcttcaaca aataaatttg aatactaatt aaagagccaa ccgttgttat agtctaacaa
     5341 tggttggctc ctcttatttt atatggtaaa aatttatagg caattttatt acaacaatgc
     5401 acatttaagg tgaccttcat gccaaactcg catcacttat tcaatggaag cagcacgtct
     5461 tcacataaag taccaatccc ttacgctatg catgttgccc cgctttgcat aaagtactta
     5521 tcgttaactc aacgcaagtt gttcctattc acaaaaagca catacagtta tctgcatgga
     5581 gcacattttc attgaaagcg cataccgctc attcaacgta tatcacacat cttcaaagat
     5641 tgaaagttcc catgcgcaca ccacgtttca tactagctat gcaactcaac ttggttcata
     5701 aactctttaa tataagtcaa agtttcaacc atcgctggtg gtcttggcac atgtccttct
     5761 gccatttgat aaaatgtttc atgcgtagca ccttttaatt ctagttggtc cgctaaataa
     5821 tacgcatgat gaataccaac ttgctggtct ttccctccat gtacaattaa tatcggcgga
     5881 ctgttttcat taatgtttgg aatcgcttgg cgtgcctcat atgccgctcg atcttttttc
     5941 ggatgaccaa tcattctacg taacatccct cttaaatcaa cgcgctcttc atacatcaaa
     6001 tcaatatctg agactccacc ccagatgata taactattta caggtaagtc ttgaaacgtc
     6061 aacaaccctt gtagtccacc tcttgaaaaa ccaaccatat gaataaaagc ttgcggatat
     6121 ttatcatgta gcaatcttaa taattcagtc acatcattta aatcgccacg ataaaattcg
     6181 tctttacctt cactgccatt gttacctcgg tagtatggcc caatcactaa agtttgacta
     6241 tctgaaaatt gcattaatct acctgcgcgc acacgtccta cttggccttt gccacctcgc
     6301 aaatatacta caatgcgttt tacttcatga tgtggtgtca tcattaaagc ttttacttgt
     6361 aaatcatctg acaaatatgt aatttcttct aattgatgcg taaaagattc aattggcatt
     6421 cgtttacgtt tgataaaatc caagtgattg caccctctct acgcatttta aaatggtact
     6481 atcttgcagt aagaaactcc gttgtgtgag ttcaatatca ttgatacagt taaacaacac
     6541 tggccctgct gtttctaaat aatcgttctt gcttaccaat gattcaactt cgataaaata
     6601 tacatctttt acaaaatcag tttgatcatg tgtttcaatg gtatattgtg ctatgtaatg
     6661 aatattttta actttggcgc ctgtttcttc atataattca cgtgtaactg cttcagcact
     6721 gctttccccg cgttcccttt taccaccagg aaattcaata ccccgtaaat tatgtttggt
     6781 aaaaagcaat tgatttttaa acgttggaat agctagcaca tgattaccat ctgctacctc
     6841 attatccttt ttaaatgtca aattaacttg acgattatct ttatccctaa acttcacgcg
     6901 catcacatcc ctacattgta tgttaatata atagttaatt actatcgttg gaggcattaa
     6961 ttatgaaaaa gatattcttg gcgatgattc atttttatca acgtttcatt tcgccactca
     7021 ctccaccaac ttgtcgtttt tatccaacat gttcagagta cactagagaa gcgattcaat
     7081 accacggtgc tttcaaaggc ctttatttag gtatccgtcg tattttaaaa tgtcatccgc
     7141 ttcataaagg tggctttgac cctgttccgt taaaaaaaga caagtcagaa agcaagcatt
     7201 cacataaaca taaccattaa tatggttgta attgagttat atccactaaa ggggggcgaa
     7261 attcgagtcg cccctctttt aatatgcctg aatgcgccac cacatcttgt tcaaaataat
     7321 aacctgctgg tgtcacatct cctggataat cacctttacg agcaagcatc gctgtaaaat
     7381 aacggcttaa accatattcg tacatgccgc caataaccac ttttacacca tgacttttca
     7441 aagtatcaat tgccgtttgc actttatcaa tgccacctag acgaaatggt tttaatacaa
     7501 caactttcac attgtataat tctatcaaat taattatgtc caacaacgat gttgcctttt
     7561 catcaagggc tattggaggt attgttccat ccactacgtc atcaagcatg gagatatctt
     7621 taaatggctc ttcgatataa agaacctgtt cacgcgctaa taactgtaac tgtgtgaaat
     7681 cttgacgatc caaggactca tttgcatcta taaccaattg aaagtgaaac tctaattccc
     7741 gtaacactct aatttgatgc atgatttgag gtgtccattt taatttaatt ctggccggct
     7801 tagttgcttt taatgactct agttgtttat ttgataagcc gctcactgtc gctccatatg
     7861 ctactgaaaa tgaaggcagt acatgaaata tttgatacaa tgccatgaca atagttgccc
     7921 ttgcagcagg cgtattttcc aatgaatcta ctaattttag tgctgcttca tacgtttcaa
     7981 atgatttatt tctattatct tcgaaccatt gctcaattac atgtttcact gaagcaattg
     8041 tttcatgatc ataccaatct gtttgaaaag cgttacattc cccgaaatat gcatttcctt
     8101 tgtcatcaat caattcgata aacaaacaat cacgatgcgt taaagtgact ttcggtgtta
     8161 caatttgtga cttaaatggc tcactatatt tataaaaatg caaagctgtc aacttcatca
     8221 aatcatcctc tatacaactt atttctttgt aatttacctg ttgatgtata aggtaaagta
     8281 tcaacctttt caaagtgttt cggtacttta tatttcgcta aatgttgtga taaatatgca
     8341 atcaattgtg cctttgaaat gtcactttca ctgacaaaat ataatttagg cacttggccc
     8401 caagtatcat caggatgccc tacacatact gcgtcactga tacctggaaa ttgcttcgct
     8461 accgtttcaa tttgatatgg ataaatattt tcaccgccac taataattaa atctttacgt
     8521 cggtcataaa tcatgacata accttcatga tctatttcag caatgtcacc tgtattaaaa
     8581 taaccatttt caaacgtacc cgttaaatct gttggataca aatatccatt catcacattg
     8641 gcgcctttaa tcattaattc tccatgacct tctttattag gatttttaat ttttacgtct
     8701 acattggcac ttggcatccc tacagtgtca ggacgtgcat gcaacatttc cggtgttgct
     8761 gttaaaaatt gcgaacatgt ctcagtcata ccaaatgaat tataaattgg caggttatat
     8821 tgtaatgccg tctctatcat agtggcagat aatttagcac cgccgagtaa tattttttgt
     8881 aaattataag gttcatgtaa accttgttgc ataagccaat ttaaagtttg tggcacaagc
     8941 gaaatgtgcg tgattcgttc atttttaatc atcgttaaaa tttgttcggc attgaattta
     9001 tcaacaatgc gcacagtaaa accttcaata acagctctta aaagtacact gagacccgaa
     9061 atatgataaa tcggcaagac agatagccaa ttagtgtcac gatcaaatcc caagctctct
     9121 ttacatccga ttgcactggc ataatgatta cgaaacgttt gtggcaccgc tttttgaggg
     9181 cccgttgtcc ctgatgtaaa cataatcgat gcaatgtcat ctaaattaaa tgatgtattt
     9241 aatatgttgg acggcgactc tttcggcacc acagtttcat tcgatgtatc atattggata
     9301 cccattgtgt tgtccaacaa accgttcgtt gtaatatccc ttccagcgaa ttcaatatca
     9361 tccagcgata caatttgaaa ccctcgtaat tccagtggca aggtacaaaa aatcaattgt
     9421 acatcgattg acttcatctg attcgtcatc tcattaggtg tcaaccttgt attaatcatc
     9481 gcaatttcaa tatttgccaa ccaacatgca tgtattaaaa tgatcgattg aatcgaatta
     9541 tctatgtata gcccaacacg agattgttga taagccttga gtcttttagc caatagactc
     9601 gcttcacagt ataaattttg ataagtataa gattcttgac cgtctgttat cgcaatatga
     9661 tgtccatttt gttgtgcttg tttatataac caaaagtcca tgcgttattc ctccaaaatc
     9721 atttacatta taattataac gattttatga cattctagca gtggttatgt ttaaaaatat
     9781 aaaaaagtag acgaattgat gcattgatat gattgttata atgctcaata catatcgtta
     9841 tatcattcgt ctactattat cagttatttt tatttaattt tagtgtcatt ctgtcatttt
     9901 gatgtggtga tttacccatt gttgccacat catctgcaat gtcaattggt atacggttca
     9961 tgtcttgtaa tgcacttaaa tggaatactt catcatctaa attttcaatg agatatacat
    10021 aatatgttac cttgtccttt ttatatttta acgttttcca aaagtccggc ttgcaattca
    10081 atacattatc cggaatatat tcaataaata agtaacgttt gctgcctact ttgtctatga
    10141 aatattttgc agtgcctttt tctatacctc ttatatgtgc atagtctgct gaaaagtaaa
    10201 tactacctat tgtttcatta tgttgttgta tttcaaatcg ttggcctact attttattat
    10261 ttgtgccaca actacttaaa aaaatcagta gatatagcat taaacatatt ttcatcccct
    10321 tgaattttaa aaactttttc aaaagcaaca cctctaaaaa taaatacaat atattatagc
    10381 aaatagaaat tattattttg ttaaattaag ttgtcatgtt cgtgtcattt tgagtcgtaa
    10441 tacaacttta caattattaa attaacactt ctacaatcca aaaatatttt taaagttttg
    10501 aaaaaattaa agtatttcca ctactaattt ggtaaaatat gattatgtga tatagtgaaa
    10561 gcgttttaca cttaataact ccctcttaaa tgcatccagg ttttatgtag taaatcatga
    10621 aaataacata taaatataga ggagatttac ctttgaatac agagaacaac aagaatcaaa
    10681 accaatctgt taaaaattct gaaagacgcg gcatgttaaa aggatgcggc ggttgcctta
    10741 tttcttttat tttattaata atcttattat cagcctgttc aatgatgttt agtaataatg
    10801 acaatcccac tagtaatcaa tcatcaaaaa cgcaattaac tcaaaagatg aaaataaaaa
    10861 tgaagataag cctgaggaaa aatcagaatc agaaacagat aaggatttac aatcaaccga
    10921 agaagtaccc gcaaatgaaa atactgaaaa taatcaacat gaaattgatg aaataacaac
    10981 aaaagatcaa tcagacgatg atattaacac accaaacgtt gcagaagata aatcacaaga
    11041 cgacttgaaa gatgatttaa aagaaaagca acaatcaagt aaccatcatc aatctacgca
    11101 acctaagacc tcaccatcaa ctgaaacaaa cacgcaacaa tcatttgcta attgtaagca
    11161 acttagacaa gtatatccga atggtgtcac tgccgatcat ccagcatatc gaccacattt
    11221 agatagagat aaagataaac gtgcatgtga acctgataaa tattaaacaa caagcgaatt
    11281 gaattcaaat tgtatttagc tttatgcact aatcacatag taaataatga gggagatttt
    11341 ttaggcatga gcaatcaatt caaaagcgaa gaagagcgaa gacaatggga acaattccaa
    11401 gctttccaaa atcaacaaaa ccaacagaac cagcaatacg gacaaaagaa atctaaaaaa
    11461 ggatggttct ggggctgtgg tggttgtcta gtattattta ttttaattat catcggtatt
    11521 tcagcttgta cagctggtat tacaggtaac cttggcggaa atagttctaa agaaacgaac
    11581 aaaacccata aaatcggtga aactgttaaa aatggcgacc ttgaagtcac tgtaaattca
    11641 gtggaaacta tgaaatctgt aggaccgtct cttgcaccaa caaacgctaa aggtacattt
    11701 gtcgttgctg atgtgacaat taaaaacaaa ggtaaagaag cgttaacaat tgatagttca
    11761 atgtttaagt taaaatccgg tgataaaaca tttgaagcag ataatacagg ttcaatgtct
    11821 gctaatcaaa gtgacaatgg tagtatagaa aattcatttt tcttacagcg tataaatcct
    11881 gatagcactg cacaaggtaa aattgttttc gatgtgtcag aaaacatagc caacgcaaaa
    11941 gataaaaaat tagaagttat ttctagttta tttagcgtca agaagattac atttgattta
    12001 tccgaagcta aaaaaacatc aaaagctaaa aaagacaagc aagatacaga agtagctgtt
    12061 gcgagttcaa atagcgataa tataagttat gaagcttcgg ctactacacc tgctacaact
    12121 tctagtgcgg atactgattc tgaagatagc gaaaagtcta gtaaagatga ggataagcag
    12181 aatgcgtcta aaagtgataa atctagtgta gaaaaaagtg aatctaatga ggaaactgct
    12241 cctgtagagc ctatgcgcca gagcaaacct accactagtg aagcaccacc tagtcaaaat
    12301 attcacaatg aagatagcat gtacgacgct tcaacagaat aaaatttcag ggtagctcgg
    12361 ctacccttct tttacggaaa aattaattat acataatcaa atcaaggaga taaaaaaatg
    12421 aaattcaaag ctatcgttgc aatcacatta tcattgtcac tattaactgc ttgcggtgct
    12481 aatcaacata aagaaaataa tagtaaatca aataacacta ataaaaagac gcaacaaact
    12541 gacaacacta caaagtcaaa tacagacaag caaatgacac cacaagaagc cgaagatatc
    12601 gttcgaaacg attacaaagc aagaggtgct aacgaaaatc aaacattaaa ttataaaaca
    12661 aatcttgaac gaagcaatga acatgaatat tatgttgaac atctagtccg cgatgcagtt
    12721 ggcacaccat taaaacgttg tgctattgtt aatcgacaca atggtacgat tattaatatt
    12781 tttgatgatg tgtcagaaaa agataaagaa gaatttgaag catttaaaaa gagaagccct
    12841 aaatacaacc caggtatgag tgatcaagct gaaatggata atgagtcgga agacattcaa
    12901 catcatgata ttgacaataa caaagccatt caaaatgaca taccagatca aaaagtcgat
    12961 gataaaagtg ataaaaatgc tgttaataaa gaagaacaac acgataacgg tacaaataat
    13021 tctgaagaaa ctaaagttaa ataaggatat actttgatta atcgtaattt ttaggagaac
    13081 cactatgata acatttgaaa atatacaaca acttgaaaaa tataccttaa tgactatgca
    13141 tggtcttttt aatcaactta aactcggtat tatttcaatc gacaatgcag agcataccct
    13201 ctttacacct tatatgatgg aatcactctc ttccctaggt gtgaaaccag acattatcga
    13261 tttaattcac aaagggacag agttagaaga ctttgcagca tttaatttat caattgatga
    13321 tacagttaca gtctgtttac aaagaactga agaactatta aaacaataca aaaatgtaga
    13381 attcaatgac aaaatattaa tcaattggcg cgttatacaa aagtaataga catataaaag
    13441 tcgaacgtaa ctaagtgggt attgatttta ttctttctaa gttacaagta tttcatttta
    13501 taaagtttga aaagaggttt attgaaatgg agaaaaacga atatacagct aaatataatg
    13561 aatataatca attattagac gctacttact cgcaagctgt agcatacctt ttaaataaat
    13621 atggcgctgt aaccgatgac tattataagg aaaaatcata cacacgattt ttaaatggag
    13681 aaatcaaaag tattacaaaa ggaaaataca ctagagctag cgaaggatta tattgtcatc
    13741 acataagcga agacaaattc caaaacttat ctgacctaag attcatttcc gaatttaagt
    13801 actcatacaa ctatcaaaag aaagaaaact tagtttactg cgatttaatc gagcatttaa
    13861 ttttacatgc aattattaca aaagaatctg atggtcaatt tggcgcggct ggattatgtc
    13921 aaatgatcaa accaacagtc attgattggt acattagcga atataatcca aaaccagcat
    13981 ggatgcaagc caccaaagca cgtgcctatt tgcctagaat attagtagag aaactactca
    14041 ttaaaattga cgatatgtta gaaggaatag aaatatatga tttccttgag tctagataaa
    14101 tgattaatgg agatttattt tttgctgttg agattttgtt atagatgttt aaacctgtaa
    14161 ttaaatatat tttataaaat agaccacgca tacctatcta taaacggaca atgtttataa
    14221 atgagtttgc ctggtcttga attgtattag ttttcttttg gttttaataa atcgactaga
    14281 ttttcacaat atttatcaaa tatgtattcc taaattatac agccttaatc cagcagctac
    14341 tttcgaaact tccaacttag ttgatataag gttcaatagt ttgtttcgtt ctttttcaga
    14401 taaaccagaa cttaaattga tattattgac ttcataaaaa ttatagacta atgcctctat
    14461 ttgcttttta ggcataagta agtcgactga aaactgattt acgtcgcttt cataaatcat
    14521 ttcatgtaaa ttctttagac tattatcgtt gttatctctc attaagtctg aatttttaaa
    14581 taaataacgg cccaattcac gagctattgc aaatcttgta ttattaatcg agtgattatt
    14641 attaatatag attgttcttc cacttaaata acccgaagta ttaccctcca ttttaatata
    14701 tttaacattt aaattaagtt gaaataatag cttgtctatg tcaatagcaa agtgttcaga
    14761 agtaataaaa agttgatcca ttttgtcctt tataaatgcc tgaaataatc gaactatttt
    14821 tgattctaaa atatcttcat aatgaacttt ctcaataact ttcaattgat tccccaccct
    14881 aactaaaaaa ttgaacctac atcatttaat aaatttaaaa ctataggtgt aaacattatt
    14941 aaaataaaac tcctccacta ctaaatgaaa cggagggaca tgatttcaaa ataaaatacc
    15001 tttttttata aatttattat aatattttcc attatactgt acaaccgaat ctaatccaac
    15061 gatcccgaaa agaagaacaa aggtggttct gcttcataat cacttttaac taagtgttca
    15121 tttttaatta tttgatcgac ggttttccaa ttttttactt cttgctcttt taaaaaatgg
    15181 ctatgaaaaa ttctatcctc atagattttt tcatagccaa tgctaatttg tttcatgtca
    15241 tttctttaaa attcctaaaa atatataaac taaaagcata agtaacacag caaaaattgc
    15301 cgaatatata attattgaaa aagcattgct ttgcattgga actaaataat ttaaagtaca
    15361 aaataataat aatgctaaaa taccaacttt aagtgaatat tttaatctat ccataaaatc
    15421 acccttactg atatccccat ttttctccta tcagaacgta ttccccggct gcattctttt
    15481 tagtttttaa ttttaaatat attctcttac tagtatcaat attcgatgca gctatagatc
    15541 caaggaaact accaacacct gcgccaacta aacctcctgt tagtgcagaa ataacgctaa
    15601 tagtccccgc acctaaagca gctattgttt ttgtatatgc agaagaaaga tataacgttg
    15661 cagtatcttt acctgtttct acatattgag ttttacccgc tcttaattgg tcttcagctt
    15721 taaatttttt tatttcttct tttgtaaata tatcttccag tttataacct tttttctcaa
    15781 gttcatcaaa taaattttgg ttactcaaat atataacctt tgcttgagaa tggtctaact
    15841 tatcttcagc atgagctaca tctgaattat aagataatga aattggacta acaaataata
    15901 caccagcagc taataataat gatttttaat tcgtttttca ttagtttctt ttagatgatt
    15961 tttgtattta gctttcgtat aaacagaaac tagattatgt ttttcatgat acgacctatc
    16021 tttcgtcttg atacagagag acccttatca tttaaattat ttttaattcg tcctgtacca
    16081 aagacttttc tattagaatt aaaaatattt atgacggcac gttctacgtt tgaatcatct
    16141 ttagtgattt tattatcttt tctttttata gaatcataat aggtacttct tagtattttt
    16201 aagactttac ccattgctga tactgaatat tgatgtgcat tcttttgaat gacttctatt
    16261 ttcgtcccat aatcagcgct gcttgcttta aaatatcgtt ctccattttt aaatgttgaa
    16321 cttctttgtg taatttaatc agctcttttt cttcatctgt taagttatct tgatgattga
    16381 atgaacctta tttttgatgt tgctttatcc attttcctaa cgtcaaaggt gttaaatcat
    16441 actcgcgtat aatttcattt cttggcttac cattttcata taatctaacc atttataact
    16501 taaactctga actaaatgtt cttctttctt aaattaacaa aatctactct catagaattt
    16561 gtccaattag atgatataat agtagagtcg cctatctctc aggcgtcaat ttagacgcag
    16621 agaggaggtg tataaggtga tgcttatttt cgttcacatc atagcaccag tcatcagtgg
    16681 ctgtgccatt gcgttttttt cttattggct aagtagacgc aatacaaaat aggtgacata
    16741 tagccgcacc aataaaaatc ccctcactac cgcaaatagt gaggggattg gtgtataagt
    16801 aaatacttat tttcgttatc tcaattatac tgctaatttt tctttttgta aaatatgcaa
    16861 ggttttaaag agaaacatca agaactaaag agtgctctat gccaaattgg actgatgcgt
    16921 tcaatatccg aagttaggca actaaacatt gcttagcttc ctttttactt tttggagcgt
    16981 aaagttttaa acataataat attcgatttc gcaaatgatt ataacttcct taaccaaaag
    17041 atgtacgttt aattaaattt tgttgtttat cccttctaaa ggaccatttg ataaattgta
    17101 ataatcaatg gttacactat taaaagtgtc acaaattctt atgaatctgg cataaacttt
    17161 gaattaacta aataagtaag aaaacctcgg cactatatca ttttaatag
//
LOCUS       DAHHOJ010000053        17198 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.053, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000053 DAHHOJ010000000
VERSION     DAHHOJ010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 17198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17198
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.053"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(67..477)
                     /locus_tag="NBH32_001522"
     CDS             complement(67..477)
                     /locus_tag="NBH32_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HCD1515118.1"
                     /translation="MNTNDAIKILKENGLKYTDKRKDMLDIFVEEDKYINAKYIQQVM
                     DENYPGISFDTIYRNLHLFKDLGIIENTELDGEMKFRIACTNHHHHHFICEKCGDTKV
                     IDYCPIDQIKLSLPGVNIHKHKLEVYGVCESCQD"
     gene            complement(464..1327)
                     /locus_tag="NBH32_001523"
     CDS             complement(464..1327)
                     /locus_tag="NBH32_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HCD1515119.1"
                     /translation="MIDALLNFDFMRYSLISGILIGFIAPLIGAFIVVRRLSLIADAL
                     SHVTLGGISFGMFLLTIMPTLVFINPMWFGILFAIVGALLIEKLRTSYTAYQEIAIPI
                     IMSAGIALSAIFISLADGFNQEIVGLLFGSISAVNISDLTTIIVIAIIVVLFITLFYK
                     ELFILSFDEEYSKVIGIPKWIQFLFIVIVAMVISASMRVVGILLVSALITLPIAISMR
                     ITKSFKQLILLSVFLGELSVILGLVLAFYMDISPGGVIVVLLVILLMITMAYQKMRMK
                     FKKGANINEYK"
     gene            complement(1369..2154)
                     /locus_tag="NBH32_001524"
     CDS             complement(1369..2154)
                     /locus_tag="NBH32_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HCD1515120.1"
                     /translation="MRGENTMTPVFELKNVNYYYDHKKVLENINIKINKGEFLAIVGP
                     NGAGKSTLLKLILGLLPLQSGEIFVEGIDFKNKKTSIKLSYVSQKANAFNSGFPASVK
                     EVVLSGLTKTKRLFQTFNSKDNEKVIKVLERLNISDLIHKNIAELSGGQQQRVMIARA
                     LISEPAVLVLDEPTNGIDAKHVSEFYNTLDQLKQEGITIILVTHDIGVVADTATEVAC
                     LNKHLHFHGTTDEFKSLDEVEISKIYGHPVRFVDHQHNRECCN"
     gene            complement(2280..3170)
                     /locus_tag="NBH32_001525"
     CDS             complement(2280..3170)
                     /locus_tag="NBH32_001525"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCD1515121.1"
                     /translation="MLLGSHVSMSGKKMLEGSAIEAHEYGETTFMIYTGAPQNTRRKS
                     IEDLNITKGHEVMEKYGLSNIVVHAPYIINIANTTKPETFNLGVDFLQQEIERTQAIG
                     AKDIVLHPGAHVGAGVDAGINKIIEGLNEVLTNDNNVRIALETMAGKGTEIGRSFEEL
                     ARIIDGVHNNERLSVCFDTCHTHDAGYNVKEDFDGVLNEFDKIIGVDRIKVVHVNDSK
                     NDRGAQKDRHENIGFGYIGFDALNYIVHHDSFKDIPKILETPYVGEDKKNKKPPYKLE
                     IEMLKQQQFDPELKNKVMQQ"
     gene            complement(3180..4526)
                     /locus_tag="NBH32_001526"
     CDS             complement(3180..4526)
                     /locus_tag="NBH32_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCD1515122.1"
                     /translation="MAKHPFEQFNLESSLIDAVKDLNFEKPTEIQNRIIPRILKRTNL
                     IGQSQTGTGKSHAFLLPLMQLIDSEIKEPQAIVVAPTRELAQQLYDAANHLSQFKAGV
                     SVKVFIGGTDIEKDRQRCNAQPQLIIGTPTRINDLAKTGHLHVHLASYLVIDEADLMI
                     DLGLIEDVDYIAARLEDNANIAVFSATIPQQLQPFLNKYLSHPEYVAVDSKKQNKKNI
                     EFFLIPTKGAAKVEKTLNLIDILNPYLCIIFCNSRDNANDLARSLNEAGIKVGMIHGG
                     LTPRERKQQMKRIRNLEFQYVIASDLASRGIDIEGVSHVINFDVPNDIDFFTHRVGRT
                     GRGNYKGVAITLYSPDEEHNISLIENRGFVFNTVDIKDGELKEVKAHNQRQARMRKDD
                     HLTNQVKNKVRSKIKNKVKPGYKKKFKQEVEKMKRQERKQFSKQQNRQKRKQNKKG"
     gene            complement(4640..5740)
                     /locus_tag="NBH32_001527"
     CDS             complement(4640..5740)
                     /locus_tag="NBH32_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HCD1515123.1"
                     /translation="MKIADLMTLLDHHVPFSTAESWDNVGLLIGDEDVEVTGVLTALD
                     CTLEVVNEAIEKGYNTIISHHPLIFKGVTSLKANGYGLIIRKLIQHDINLIAMHTNLD
                     VNPYGVNMMLAKAMGLKNISIINNQQGVYYKVQTYIPKDNVGPFKDKLSENGLAQEGN
                     YEYCFFESEGRGQFKPVGEANPTIGQIDKIEYVDEVKIEFMIDAYQKSRAEQLIKQYH
                     PYETPVFDFIEIKQTSLYGLGVMAEVDNQMTLEDFAADIKSKLNIPSVRFVGKSNQKI
                     KRIAIIGGSGIGYEYQAVQQGADVFVTGDIKHHDALDAKIHGVNLIDINHYSEYVMKE
                     GLKTLLMNWFNTEKINIDVEASTINTDPFQYI"
     gene            complement(5743..6420)
                     /locus_tag="NBH32_001528"
     CDS             complement(5743..6420)
                     /locus_tag="NBH32_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HCD1515124.1"
                     /translation="MISLNNRLTTVSRFLKQGTIADIGSDHAYLPIYAIQNHLCECGI
                     AGEVIQGPFQAAVKNVAANQLEDRIDVRLGDGLSVIHPEDVIDNITICGMGGPLIAKI
                     LKDGQDKLSQHPRLILQSNIQTENLRQTLQQLNYEIIDEIIMEEKGHIYEIVVAEYST
                     QLIELSSDELKFGPKLLNNKNEYFIKKWQRELEALYHIKSKLNTEQHHQRLAQINDEI
                     AVIERVL"
     gene            complement(6551..7657)
                     /gene="rpoD"
                     /locus_tag="NBH32_001529"
     CDS             complement(6551..7657)
                     /gene="rpoD"
                     /locus_tag="NBH32_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCD1515125.1"
                     /translation="MSDNTVKIKKQTIDPTLTLEDVKKQLIEKGKKEGHLSHEEIAEK
                     LQNFDIDSDQMDDFFDQLNDNDISLVNEKDSSDTDEKLNPSDLSAPPGVKINDPVRMY
                     LKEIGRVNLLSAQEEIELAKRIEQGDEVAKSRLAEANLRLVVSIAKRYVGRGMLFLDL
                     IQEGNMGLIKAVEKFDFNKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINK
                     LIRVQRQLLQDLGRDPAPEEIGEEMDLPAEKVREILKIAQEPVSLETPIGEEDDSHLG
                     DFIEDQEAQSPSDHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVGK
                     VFGVTRERIRQIEAKALRKLRHPSRSKRLKDFMD"
     gene            complement(7881..9698)
                     /locus_tag="NBH32_001530"
     CDS             complement(7881..9698)
                     /locus_tag="NBH32_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCD1515126.1"
                     /translation="MRIDQSIINEIKDKTDILDLVSEYVKLEKRGRNYIGLCPFHDEK
                     TPSFTVSEDKQICHCFGCKKGGNVFQFTQEIKDISFVEAVKELGDRVNVAVDIEATQS
                     NSNVQIASDDLQMIEMHELIQEFYYYALTKTVEGEQALTYLQERGFTDALIKERGIGF
                     APDSSHFCHDFLQKKGYDIELAYEAGLLSRNEENFSYYDRFRNRIMFPLKNAQGRIVG
                     YSGRTYTGQEPKYLNSPETPIFQKRKLLYNLDKARKSIRKLDEIVLLEGFMDVIKSDT
                     AGLKNVVATMGTQLSDEHITFIRKLTSNITLMFDGDFAGSEATLKTGQHLLQQGLNVF
                     VIQLPSGMDPDEYIGKYGNDAFTTFVKNDKKSFAHYKVSILKDEIAHNDLSYERYLKE
                     LSHDISLMKSSILQQKAINDVAPFFNVSPEQLANEIQFNQAPANYYPEDEYGGYDEYG
                     GYIEPEPIGMAQFDNLSRQEKAERAFLKHLMRDKDTFLNYYESVDKDNFTNQHFKYVF
                     EVLHDFYAENDQYNISDAVQYVNSNELRETLISLEQYNLNDEPYENEIDDYVNVINEK
                     GQETIESLNHKLREATRIGDVELQKYYLQQIVAKNKERM"
     gene            complement(9759..10577)
                     /locus_tag="NBH32_001531"
     CDS             complement(9759..10577)
                     /locus_tag="NBH32_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HCD1515127.1"
                     /translation="MEKIKIIVASDSIGETAELVARAGISQFNPKQCKNELLRYPYIE
                     SFEDVDEVIQVAKDTNAIIVYTLIKPEMKQYMSEKVAEFQLKSVDIMGPLMDLLSASV
                     EEKPYNEPGIVHRLDDAYFKKIDAIEFAVKYDDGKDPKGLPKADIVLLGISRTSKTPL
                     SQYLAHKSYKVMNVPIVPEVTPPDGLYDIDPKKCIALKISEEKLNRIRKERLKQLGLG
                     DTARYATEARIQEELNYFEEIVSEIGCPVIDVSQKAIEETANDIIHYIEQNKSK"
     gene            complement(10588..11211)
                     /locus_tag="NBH32_001532"
     CDS             complement(10588..11211)
                     /locus_tag="NBH32_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001797828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1515128.1"
                     /translation="MELSQRQERIIEIVKTKGPITGEQIADKLNLTRATLRPDLAILT
                     MSGFLEARPRVGYYYSGKSKGKFFNEKLRQFEVKDYMSQPVVLRENTTVYDAICTIFL
                     EDVSTLFIINEDNDFVGVCSRKDLLRASMIGADIHTVPISVNMTRMPNVTYLEESELV
                     IYAADRMIEKEIDSIPIVRKKDNQKYEVIGRISKTTIAKLLVALYKE"
     gene            11546..12937
                     /locus_tag="NBH32_001533"
     CDS             11546..12937
                     /locus_tag="NBH32_001533"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HCD1515129.1"
                     /translation="MAKDMDTIVSLAKHRGFVFPGSDIYGGLSNTWDYGPLGVELKNN
                     VKKAWWQKFITQSPFNVGIDAAILMNPKVWEASGHLNNFNDPMIDNKDSKIRYRADKL
                     IEDYMQDVKGDENFIADGLSFEQMKKIIDDEGIVCPVSKTANWTEIRQFNLMFKTFQG
                     VTEDSTNEIFLRPETAQGIFVNYKNVQRSMRKKLPFGIGQIGKSFRNEITPGNFIFRT
                     REFEQMELEFFCKPGEEIEWQNYWKTFASDWLTSLNMSSENMRLRDHDEDELSHYSNA
                     TTDIEYKFPFGWGELWGIASRTDFDLRKHAEHSGEDFRYHDPETNEKYIPYCIEPSLG
                     ADRVTLAFLCDAYDEEGVEGSKDARTVLHFHPALAPYKAAILPLSKKLSGEAIKIFEQ
                     LSSKFSIDFDESQSIGKRYRRQDEIGTPYCVTFDFDSLEDNQVTVRDRDSMEQVRMPI
                     SELEAFLTEKTKF"
     gene            complement(13088..13834)
                     /gene="recO"
                     /locus_tag="NBH32_001534"
     CDS             complement(13088..13834)
                     /gene="recO"
                     /locus_tag="NBH32_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCD1515130.1"
                     /translation="MRQKGIIIKAVDYGESDKIITILNEHGAKVPLMARRAKKVKTGL
                     QAQTQLFVYGLFIYNQWRGMGTLNSVDVISQHYKLQMDLYVSSYASLAAETIERSMDE
                     GDIAPYNYQLLQFVLEKIESGTSAQLMSVVVMLKCMKRFGFTASFNRCAVSGNDTQAD
                     LIGYSFKFDGAISRQEASKDVHAVILSNKTLYLLDVLQKLPIDKMNSLNIHQEIIDEM
                     SDIILMLYREYAGMFFKSQKLINQLKRLEQ"
     gene            complement(13862..14761)
                     /gene="era"
                     /locus_tag="NBH32_001535"
     CDS             complement(13862..14761)
                     /gene="era"
                     /locus_tag="NBH32_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000134760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCD1515131.1"
                     /translation="MTEHKSGFVSIIGRPNVGKSTFVNRVIGHKIAIMSDKAQTTRNK
                     IQGVMTRDDAQIIFIDTPGIHKPKHKLGDYMMKVAKNTLSEIDAIMFMVNANEEIGRG
                     DEYIIEMLKNVKTPVFLVLNKIDLVHPDELMPKIEEYQSYMDFTEIVPISALEGLNVD
                     HFIDVLKTYLPEGPKYYPDDQISDHPEQFVVGEIIREKILHLTSEEIPHAIGVNVDRM
                     VKESEDRVHIEATIYVERDSQKGIVIGKGGKKLKEVGKRARRDIEMLLGSKVYLELWV
                     KVQRDWRNKVNFIRQIGYVEDQD"
     gene            complement(14762..15166)
                     /gene="cdd"
                     /locus_tag="NBH32_001536"
     CDS             complement(14762..15166)
                     /gene="cdd"
                     /locus_tag="NBH32_001536"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCD1515132.1"
                     /translation="MSYQPHYFQEVRKAQQESYSPYSQFKVGAYLKTKDGRTFYGTNV
                     ENASYPLSICAERASLVSAISQGYRPGDFESITVTVDADKPSSPCGACRQVLKELCDD
                     DMPVYMTNHKGDMVMMTVAELLPFGFSGKDLE"
     gene            complement(15177..15521)
                     /locus_tag="NBH32_001537"
     CDS             complement(15177..15521)
                     /locus_tag="NBH32_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCD1515133.1"
                     /translation="MKRFKYALDGLKILIQKDYKFLLHVFAMIVAIVFGLVLNINRIE
                     WIFILIAIALVLTVEALNTAIEYVVDLVTVEYHDLAKYAKDIAAFSVLIVSILAFIIG
                     LIVFLPHFIALF"
     gene            complement(15524..15991)
                     /gene="ybeY"
                     /locus_tag="NBH32_001538"
     CDS             complement(15524..15991)
                     /gene="ybeY"
                     /locus_tag="NBH32_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCD1515134.1"
                     /translation="MFTIDFSDHTGLVKDAWYKQIEDLLEFAKKEEHIEDDAELSVTF
                     VDKQEIQEINRTYRDKDKVTDVISFALEEDEPEIDFSDLDIPRVLGDIIICTDVAQEQ
                     ANNYGHSFERELGFLALHGFLHLLGYDHMTEADEKEMFGRQDTILNAYGLTRD"
     gene            complement(15992..16939)
                     /locus_tag="NBH32_001539"
     CDS             complement(15992..16939)
                     /locus_tag="NBH32_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCD1515135.1"
                     /translation="MPGIIQIDDMNQSQALIGNNDEHLKAIEESFDVVIHARGQEVAV
                     KGTKIENVEKAESVLINLLKVIDLGNNITIKDVEAAIKMAHNNTIQHLLDLYDEEITK
                     DAFGKTIRAKTMGQRIYVNAMKNNDLVFGIGPAGTGKTFLAVVYAAKQLRKGAVKRIV
                     LTRPAVEAGESLGFLPGDLKEKVDPYLRPLYDGLYTVLGREQTERFIERGIIEIAPLA
                     YMRGRTLEDAFVILDEAQNTTHAQMKMFLTRLGFGSKMVVTGDQTQIDLPKGVKSGLK
                     EAVSRLHNVKGISILKLDQSDVVRHPLVSKIIEHYEGEN"
BASE COUNT         5406 a         3187 c         2311 g         6294 t
ORIGIN      
        1 tataatttaa aaaatgagat ttatgcatac atctgaccta atgtgtataa atctcatttc
       61 tttatattaa tcttggcaag actcacatac accataaact tcaagtttgt gtttgtgaat
      121 attaacacca ggtagtgata attttatctg atctattgga caataatcta ttacctttgt
      181 atctccacac ttttcacaga taaaatgatg atgatgatgg tttgtacaag cgattctaaa
      241 tttcatttca ccatcaagtt ctgtattttc aataatccct aaatctttaa ataagtgcag
      301 gtttctatat attgtgtcga atgaaattcc aggataattt tcatccataa cttgttgtat
      361 atactttgcg tttatatact tatcttcttc gacaaaaata tctaacatat ctttacgttt
      421 atctgtatat tttaaaccgt tctcttttaa aattttaata gcatcatttg tattcattga
      481 tattagctcc ctttttaaac ttcattcgca ttttctgata agccattgta atcataagta
      541 aaataaccag tagaactaca attacaccac ccggagaaat gtccatatag aaagctagga
      601 ctaagcctaa tattactgat aattcaccta aaaatacact tagtaatatc aattgcttaa
      661 aactttttgt tattcgcata cttattgcaa ttggtaacgt gattaacgca cttactaaca
      721 gtatccctac aacacgcatt gaggcagaaa taaccatcgc tacaataaca ataaataaaa
      781 attgaatcca tttaggaatg ccaatgactt tactatattc ctcatcaaat gacaatataa
      841 ataattcttt ataaaacaat gtaataaaca gaacaactat gatggcaatg acaataatcg
      901 ttgttaaatc acttatattc actgcgctta ttgagccaaa tagcaatcca acaatttctt
      961 gattgaaccc atcagctaat gaaatgaaga ttgcactcaa ggcgatacca gcactcatta
     1021 taattggaat agcaatttct tggtaagcag tgtatgacgt tcttaatttt tcaattagaa
     1081 gcgcacctac tattgcgaat aagattccaa accacattgg attaataaat actagtgttg
     1141 gcataatagt aagtaaaaac ataccgaagg atataccacc taaagttaca tgacttagag
     1201 catcagctat aagtgatagt cgtctaacaa cgataaaagc accgattaga ggcgcaataa
     1261 aacctatcaa gataccacta attaaagagt acctcataaa atcaaaattc aataatgcat
     1321 ctatcaattg tgacacgcct ttccatttta tataaactca aatctttatt aattacaaca
     1381 ttctcgatta tgctgatgat cgacaaaacg tacaggatgt ccataaattt ttgaaatttc
     1441 aacttcatca agtgatttaa actcatcagt tgtaccatgg aaatgcaaat gcttatttaa
     1501 acatgctact tcagtagcag tatctgctac aacaccaata tcatgagtaa ctaagataat
     1561 agtgatacct tcttgtttta attgatctaa agtattataa aattcactta catgttttgc
     1621 atcaatacca ttcgtcggtt catcaagtac taatactgca ggttctgaaa tcaatgctcg
     1681 agcaatcatt acacgttgtt gttgaccacc tgataattct gctatatttt tatgaattaa
     1741 atcacttata ttcagtcttt ctagtacttt aatcactttt tcattatctt tgctattaaa
     1801 tgtttggaaa agacgttttg tctttgttaa tccgcttaaa acaacttctt taacacttgc
     1861 tgggaaacct gaattaaagg catttgcttt ttgtgataca tagcttaatt taattgatgt
     1921 tttcttattt ttaaaatcaa taccttcaac aaaaatctca ccactttgta aaggtaataa
     1981 tcctagaatc aacttcaata atgttgattt accagcacca tttggtccaa caattgctaa
     2041 aaattcacct ttatttattt taatgtttat attttctaac acttttttat gatcatagta
     2101 gtaattgaca ttcttcaatt caaagactgg tgtcatcgta ttctcacctc gcattcaact
     2161 atacaactcc tagtaacata tgtaaacagt aatgtttacg actcaaaata agacaaaata
     2221 aagagatatg cccccttcaa gttttattta tcgcatttct tgaagagagc attatcattt
     2281 tattgttgca taaccttatt ttttaattct gggtcaaatt gctgttgttt caacatttca
     2341 atttcaagtt tatatggcgg ttttttattt ttcttatctt caccaacata aggtgtttct
     2401 aagattttcg gaatatcttt aaaactatca tgatgcacaa tgtaatttaa tgcatcaaaa
     2461 ccaatgtaac cgaagccaat attttcatgt cggtcttttt gagcgccacg gtcattttta
     2521 gaatcattga catgaacaac tttgattctg tcgactccaa tgattttatc aaattcattt
     2581 aatacgccat caaagtcctc tttcacatta tatccagcat catgcgtatg acatgtatca
     2641 aaacatactg ataaacgttc gttattatga actccatcaa taatacgtgc taactcttca
     2701 aacgagcgac caatctcagt acctttacct gccatcgttt caagcgcaat acgtacatta
     2761 ttgtcattcg ttaaaacttc atttaatcct tcaataatct tattaattcc ggcatcaaca
     2821 ccagctccaa catgcgcacc tggatgtaat acaatatctt tagcgcctat agcttgcgtt
     2881 ctttcaattt cttgttgcaa gaaatctaca ccaagattaa acgtttctgg tttggttgta
     2941 tttgcaatat taatgatgta tggtgcatga acaacaatat tagataagcc atatttttcc
     3001 atcacttcat gacctttagt tatatttaaa tcttcaatac ttttacggcg cgtattttga
     3061 ggtgcaccag tataaatcat aaatgttgtt tcaccatatt catgcgcttc tatagcagaa
     3121 ccttctaaca tctttttacc actcattgaa acatgtgatc ctaataacat aaaacacacc
     3181 taaccttttt tgttttgctt acgtttttgt ctattttgct gcttactaaa ttgtttacgc
     3241 tcttgacgtt tcattttttc aacttcttgc ttaaatttct tcttataacc tggtttaact
     3301 ttgtttttaa ttttacttcg aactttgttc ttcacttgat tagttaaatg gtcatctttg
     3361 cgcattcttg cttgacgctg attgtgcgct ttaacttctt ttaactcacc atctttaata
     3421 tcaacagtat tgaaaacaaa accgcgattt tctattaatg aaatattgtg ttcttcatca
     3481 ggactataaa gcgttattgc tacaccttta taattcccac gaccagttcg tccgactcta
     3541 tgtgtaaaga agtcaatatc atttggcaca tcaaaattga tgacatgact aacaccttca
     3601 atatcaatac cacgagatgc taaatcgctg gcaataacgt attggaattc taaattacgt
     3661 atacgtttca tttgttgttt acgttcacgt ggcgttaaac caccatgaat cataccaact
     3721 ttaataccag cttcatttag tgaacgtgct aaatcatttg cattatctct actattacag
     3781 aaaataatac ataagtatgg atttagtata tcaattaaat ttaaagtctt ttcaacttta
     3841 gctgcacctt tagtaggtat taaaaagaat tcgatgttct ttttattttg ttttttactg
     3901 tcgacagcta catattctgg atgacttaaa tatttattta aaaatggttg taactgttgt
     3961 ggaattgtag cactaaacac cgcaatattt gcattatctt ccaatcttgc agcaatgtaa
     4021 tctacatctt caattaatcc taagtcaatc ataagatccg cttcatcaat aactaaatat
     4081 gatgctaagt gcacatgtaa atgtcccgtt ttagctaagt cattaattct agtaggggtg
     4141 cctataatca attgtggttg tgcattacaa cgttgtctat ctttctctat atctgtacca
     4201 ccaataaaaa ctttaactga aacgccagct ttaaattggc ttaaatggtt cgctgcatcg
     4261 tatagttgtt gtgcaagttc tcttgttggt gcaactacga ttgcttgtgg ttcttttatt
     4321 tcactatcaa ttaactgcat taatggtaat aaaaatgcat gagatttccc tgtacccgtt
     4381 tgagattgac caattaaatt tgttctcttt agtattcttg gaataattcg attctgaatt
     4441 tcagttggtt tttcaaaatt aaggtctttc acagcgtcaa ttaaactaga ttctagatta
     4501 aattgttcga atggatgttt tgccatgatt tggcctcctt atatgttcat ctttactatt
     4561 ataaagtata aatgtgctat tttaaataaa aatattaaaa cctcgaaatt tcaaaattta
     4621 gaaatcccga gattttagtt taaatatatt gaaatggatc tgtattaatt gtagatgctt
     4681 caacatctat atttattttt tctgtattga accaattcat tagtaacgtt tttaaacctt
     4741 ctttcatcac atattcgctg taatgattaa tatcaattaa gttcacacca tgaattttag
     4801 catctaaggc atcatgatgt ttaatatcac ctgtaacaaa gacatctgcg ccttgttgga
     4861 cagcttgata ttcatatcca atacctgaac caccaataat tgcaatacgt ttaattttct
     4921 gattagactt accaacaaaa cggacacttg ggatatttaa tttagattta atatcagctg
     4981 cgaaatcttc caatgtcatt tgattatcca cttctgccat aacgccaagt ccataaaggg
     5041 atgtttgttt tatctcaata aaatcaaata ccggtgtttc atatggatgg tattgtttaa
     5101 ttaattgctc agcccttgac ttttgatatg catctatcat aaattcaatt ttaacttcat
     5161 ctacatattc aattttatca atttgtccta ttgttggatt agcttcacca actggtttga
     5221 attgccctct tccttcactt tcaaagaaac aatattcata attaccttct tgtgctaatc
     5281 cattttcact aagcttatct ttaaatggtc caacattatc cttaggtata tatgtttgaa
     5341 ctttatagta tacaccttgt tgattattga ttattgaaat gttcttcaaa cccatcgcct
     5401 tcgccaacat catattgaca ccatacggat ttacatctaa atttgtatgc atcgctatta
     5461 aattaatgtc atgttgaatt agttttctaa taatcaaacc ataaccatta gcttttaatg
     5521 atgttacgcc tttaaagatt agaggatgat gactaataat agtattataa cctttttcga
     5581 ttgcttcatt tactacttcc aacgtacagt ctaatgctgt taaaacacca gtaacttcaa
     5641 catcttcatc acctattaac aatcctacat tatcccaaga ttcagcagta ctaaatggca
     5701 catgatgatc taacaatgtc attaaatcag ctattttcat tactataaca ccctttcaat
     5761 tacagcaatt tcgtcattaa tttgagctaa acgttgatga tgttgttcag tattgagttt
     5821 cgatttaata tgataaagtg cttctaactc tctttgccat ttttttataa aatattcatt
     5881 tttgttgttc agcaattttg gtccgaattt caattcatca gatgatagct ctattaattg
     5941 tgtagaatat tctgctacaa caatttcata aatatggcct ttttcttcca ttattatttc
     6001 atcaattatt tcataattca attgttgtaa tgtttgtctt aaattttcag tttggatatt
     6061 actttgtaaa atcaaccttg gatgttgact taacttatct tgcccatctt ttaaaatttt
     6121 agcaataagt ggtccgccca taccacaaat tgtgatatta tcgattacgt cctcgggatg
     6181 aataacactt aagccatccc ctaaacgtac atcaattcta tcttctaatt gatttgcagc
     6241 tacatttttc actgcagctt gaaaggggcc ttgaataact tctcctgcaa taccgcattc
     6301 gcataaatgg ttttgaattg catagattgg caaataagca tgatctgagc caatatccgc
     6361 gattgtacct tgttttaaaa atcgacttac cgtcgttaat cggttattta acgaaatcat
     6421 tttattctcc tttatgaaaa aagaggttga acattattca atgtaccaac ctcactcaag
     6481 ttaaatctta atctaaaaat aaataagcgc ttattcatgt cttggtatca atcattaaaa
     6541 ttgatataaa ttaatccata aagtctttca aacgtttact acgacttgga tgtcttaatt
     6601 ttctaagtgc ttttgcttca atttgtcgaa tacgttcacg tgtaacaccg aaaactttac
     6661 caacttcttc aagtgttctt gttctgccgt catcaagacc aaatcttaat cgtaatacat
     6721 tttcttctct atcagttaat gtatcaagca catcttctaa ttgctctttt aataattcat
     6781 aagcagcatg atctgaagga ctttgtgctt cctgatcctc aataaagtct cctaaatgac
     6841 tatcatcttc ttcaccaatt ggtgtttcta atgaaacagg ttcttgcgca atttttaaaa
     6901 tttcacgaac tttttctgct ggtaaatcca tttcttcacc aatttcttct ggtgctggat
     6961 ctcgacctaa gtcctgtaat aattgacgtt gaacacgaat taatttatta attgtttcta
     7021 ccatatgcac agggatacga atcgtacgtg cttggtcagc aattgcacga gtgattgctt
     7081 gtctaatcca ccatgttgca tatgttgaaa acttaaatcc tttgttaaag tcaaattttt
     7141 caacagcttt aataagaccc atattacctt cttggattaa atcaaggaat aacataccac
     7201 gacctacgta tcttttagca atacttacaa ctaaacgtaa gttcgcttct gcaagtcttg
     7261 attttgctac ttcatcacct tgttcaatac gtttggctaa ttcgatttct tcttgtgcac
     7321 ttaataagtt aacacgccca atttctttaa ggtacatacg aactgggtca tttattttaa
     7381 cacctggagg ggcactaaga tcacttggat tcagtttctc gtcagtatct gaactatctt
     7441 tttcattaac tagtgaaata tcattatcat ttaattgatc aaagaaatca tccatttgat
     7501 cagagtcgat atcaaaattc tgaagttttt cagcaatttc ttcatgactt aaatgaccct
     7561 cttttttacc tttttcaatt aattgcttct taacatcttc taatgttaat gtcggatcaa
     7621 ttgtttgttt tttaatttta actgtgttat cagacatgaa acggcctccc gattttaaat
     7681 atgaacattc gaaatttatt caatattgct attttaaact aaattcttaa ttaattcatc
     7741 catattttta attttatttt acaaattgga actaatccca atatttattt ttcaatagtg
     7801 ttgtttaaat gcggtaacta tcatttaccc ttatacattt tgacataatc attattcgta
     7861 ttattcttta aaatcacatg ctacatgcgt tctttattct tagcaacaat ttgctgtaaa
     7921 tagtattttt gtaattctac atcgccaatc cttgtagctt cccttaattt atgattcaat
     7981 gactcaattg tttcttgtcc tttttcatta ataacattga cataatcatc aatttcattt
     8041 tcatatggtt cgtcattcaa attatattgt tctaagctaa ttagtgtttc tctcaactca
     8101 tttgaattaa catactgtac agcatcactg atattatatt gatcattttc cgcataaaaa
     8161 tcatgtaaga cttcgaatac atatttaaaa tgctgatttg tgaagttatc cttatcaaca
     8221 ctttcataat aatttaaaaa tgtatcttta tctctcatta aatgttttaa aaatgctcgc
     8281 tccgcttttt cttgacggct caaattgtca aattgtgcca taccaattgg ctcaggttca
     8341 atgtaacctc catactcatc ataaccgcca tactcatctt ctggataata attggctggt
     8401 gcttgattga attgtatttc gttagctaac tgctcaggac taacattgaa aaatggcgca
     8461 acatcattta tagccttttg ttgcagaatt gatgacttca taagtgaaat gtcatgactc
     8521 agttctttca aataacgttc atatgaaagg tcattatgtg caatttcatc ttttaatata
     8581 cttactttat aatgtgcaaa tgactttttg tcatttttca caaaagtagt aaatgcgtcg
     8641 ttgccatact taccaatgta ttcatccgga tccatgccag atggcaattg tataacaaat
     8701 acatttagcc cttgctgtaa caaatgttga cctgttttaa gtgttgcttc actacccgca
     8761 aaatccccat caaacattaa tgttatattt gatgttaact ttcgtataaa ggtgatatgt
     8821 tcatctgaca actgtgtacc cattgttgca acaacgtttt tcaagccagc agtatcagat
     8881 tttataacat ccataaaacc ttctagtaat acaatttcat ctaattttct aattgattta
     8941 cgtgctttat ctaagttata taacaacttt cttttttgaa agataggcgt ttcaggacta
     9001 tttaagtatt ttggttcttg accggtatat gttcgacctg aatatccaac aattcttcct
     9061 tgcgcatttt tcaaaggaaa cataattcga tttcgaaatc tatcgtaata actgaaattt
     9121 tcttcgttac gtgataataa tccggcttca tatgctaatt caatatcgta accctttttt
     9181 tgaagaaaat catgacaaaa atgtgagctg tcgggtgcaa agccaatgcc tcgctcttta
     9241 ataagcgcat ctgtaaaacc acgttcttgt aagtatgtta atgcttgttc gccttcgact
     9301 gtctttgtta aagcgtaata ataaaattct tgtattaact catgcatttc aatcatttgt
     9361 aaatcatcag aagcaatttg aacatttgag ttagattgtg ttgcctcaat atctacagca
     9421 acattaactc tatcacctaa ttctttaacc gcttcaacaa atgatatgtc tttaatttct
     9481 tgagtaaatt gaaaaacatt gccacctttt ttacaaccaa aacaatgaca aatttgttta
     9541 tcttcagaaa ctgtaaatga aggtgtcttt tcatcatgaa aaggacacaa acctatataa
     9601 ttgcgtcctc tcttttctaa ttttacatat tcacttacca agtctaaaat gtcggtttta
     9661 tcttttattt cattaatgat cgattgatct attcgcaaat taaatcacct attaagcatt
     9721 agttatacta ttatacctaa ttttcgacaa aaatgaaatc atttcgattt attttgttca
     9781 atataatgga ttatatcgtt tgctgtttct tcgattgctt tttgagaaac atcaatgaca
     9841 ggacatccaa tttcacttac gatttcttca aagtaattca attcttcttg aattcgtgct
     9901 tctgttgcat atcgagctgt gtcacctagt cctaattgtt ttagtcgctc ttttctaatg
     9961 cgatttaatt tttcttcact tattttaagt gcgatacatt tctttggatc aatatcatat
    10021 aagccatctg gcggtgtcac ttctggtaca atcggtacat tcataacttt gtaactctta
    10081 tgcgctaaat actgagataa tggtgtcttt gaagttctcg aaataccaag taaaacaata
    10141 tcagctttag gtaatccttt aggatcttta ccatcatcat atttaactgc aaactctatc
    10201 gcatcaattt tcttgaaata tgcatcatct aatctatgaa cgatacctgg ctcattataa
    10261 ggtttttctt caaccgaagc tgataataaa tccattaatg gccccatgat atcgacagac
    10321 ttcaattgga attctgctac tttctcactc atatattgct tcatttcagg tttaataagt
    10381 gtataaacaa tgatagcatt tgtatctttt gcaacttgaa tcacttcatc aacatcttca
    10441 aaagattcaa tatatggata tcttaataat tcatttttac attgcttagg attgaattgt
    10501 gaaatacctg ccctagcaac taactctgcc gtttcaccta tagaatctga agctacgata
    10561 attttaattt tttccattac ttctcaccta ttctttatat aatgctacta ataacttagc
    10621 tattgttgtt ttggaaattc ttccaattac ttcatacttt tgattatctt tttttcttac
    10681 aattggaatc gaatcaattt ctttttcaat cattctatct gctgcgtata tgactaattc
    10741 gctttcctct aaataagtga cattaggcat acgtgtcata tttacactga taggtactgt
    10801 atgaatatct gctccaatca ttgaagctct taataaatct tttcttgaac acacaccaac
    10861 aaaatcgtta tcttcattaa taataaataa tgtacttaca tcttctaaaa aaattgtaca
    10921 aatagcgtca taaactgttg tattctctct tagcacaaca ggttgagaca tatagtcctt
    10981 aacctcaaat tgtcgaagtt tttcattaaa aaatttacct tttgatttac ctgaataata
    11041 atatccaact cggggacgcg cttctaaaaa acctgacatt gttaatatcg ctaaatctgg
    11101 tctaagcgtt gctcttgtta aattcaactt atctgctatt tgttcaccag taatgggtcc
    11161 tttagtttta acaatttcga tgattcgttc ttgtctttga ctgagttcta taggtcttca
    11221 cccctttttt attcacttat tattataccg atatttcttt catgtcacaa acacattaat
    11281 tttacttgcc tttaaataat ctatcaatta gaataaacgt acagcgagtt aaggatagtg
    11341 taagcttaac aataagattg gcgcaacgaa tcattttaaa ataaaagcga gtgactacac
    11401 taatttgggt ggaaccgcgg gttaaatacg taacttattt tgtattatat acaatttaaa
    11461 atattaactc gtcccatgga agtaaagcat atttactatg ctgttatttt ccatggggcg
    11521 ttttcatgta tgaggagagg taattatggc aaaagatatg gatacaattg tttcattagc
    11581 aaaacacaga ggttttgtgt tccctggtag tgatatttac ggtggtttat caaacacatg
    11641 ggattatggt cctttaggtg ttgaattaaa gaataatgtt aaaaaagctt ggtggcaaaa
    11701 attcattaca caatcaccgt ttaacgttgg tatcgatgct gcaatcttaa tgaatccaaa
    11761 agtatgggaa gcttcaggac acttaaacaa cttcaacgac ccaatgattg ataataaaga
    11821 tagtaaaatt cgatatcgcg ctgataaatt aattgaagat tatatgcagg atgttaaagg
    11881 tgatgaaaac ttcattgccg atggtttaag ttttgaacaa atgaaaaaaa ttattgacga
    11941 tgaaggtatt gtttgtcctg taagtaaaac tgctaactgg actgaaattc gccaattcaa
    12001 tttaatgttt aaaacattcc aaggtgtaac tgaagattct acaaatgaaa ttttcttacg
    12061 tcctgaaaca gcacaaggta tttttgtaaa ctataaaaac gtgcaacgtt caatgcgtaa
    12121 aaaattacca tttggtatcg gtcaaattgg taaatcattc cgtaatgaaa tcactccagg
    12181 taacttcatt ttcagaacaa gagaatttga acaaatggaa cttgaattct tctgtaaacc
    12241 tggagaagaa atcgaatggc aaaattattg gaaaactttt gcaagtgact ggttaacaag
    12301 cttaaatatg agcagtgaaa atatgcgttt acgtgatcat gatgaagatg aattatctca
    12361 ttactcaaat gcaacaactg atattgaata taaattccca tttggttggg gtgagttatg
    12421 gggtatcgca agtcgtacag acttcgactt acgtaaacat gctgaacact ctggtgaaga
    12481 tttcagatac catgatccag aaacgaatga aaaatatatt ccatattgta tcgagccatc
    12541 acttggtgca gatcgtgtaa cattagcttt cttatgtgat gcatatgatg aagaaggcgt
    12601 tgaaggtagt aaagatgcac gtacagtttt acacttccat cctgcattag caccatataa
    12661 agcagcgatt ttacctttaa gtaagaaatt atctggcgaa gcgattaaga tttttgagca
    12721 attaagttct aaattctcaa tcgatttcga tgaatcacaa tctatcggta aaagataccg
    12781 tcgtcaagat gaaatcggta caccttattg tgtaacattc gactttgatt cattagaaga
    12841 taatcaagtt acagtacgtg acagagattc aatggaacaa gttcgtatgc caatctcaga
    12901 gttagaagct ttcttaactg aaaaaacaaa attctaatta aaaagactct ccaccatatg
    12961 tggtggctat ataatttata aaaagagata ctggttagta aaattgccag tatctctttt
    13021 ctattcgaaa aaggtattca aataaacaca tctatgtggc tacttgaata ccttttatta
    13081 ttttatgtta ttgttccaat ctttttaatt ggttgattag tttctgactt ttaaaaaaca
    13141 tacctgcata ttcacgatat aacattaaaa tgatatctga catttcatca ataatttctt
    13201 gatggatatt caatgaattc attttatcta ttggtaattt ttgtaataca tctaataaat
    13261 atagtgtttt attcgataat ataactgcat gtacatcttt agaagcctct tgccttgaaa
    13321 tcgcaccgtc aaacttaaaa ctataaccta ttaaatctgc ttgtgtgtca ttaccactca
    13381 cagcacagcg attaaatgat gcagtaaaac caaatcgttt catacacttt aacataacta
    13441 cgactgacat taactgtgca gatgtacctg attctatttt ttcaagaaca aattgtaata
    13501 attgatagtt atatggtgca atgtcacctt catccattga gcgctcaata gtttcagctg
    13561 ccagagaggc ataactgctt acgtaaaggt ccatttgtaa tttataatgt tgactaataa
    13621 catctacaga atttaacgtt cccatacctc gccactgatt gtaaataaac aaaccataaa
    13681 caaacaattg cgtttgcgct tgtaaacccg tcttaacttt tttagcacgc cttgccataa
    13741 gtggtacttt tgcaccatgc tcatttaaaa tcgtgataat tttatcagat tcaccataat
    13801 caactgcttt gatgataatc cctttttggc gcattaacaa ttatcttcac cacttttaag
    13861 attaatcttg gtcttcaaca taaccaattt ggcgaataaa gttaactttg tttcgccagt
    13921 ctctttgaac tttgacccat aattctaagt aaactttaga gcctagaagc atttctatat
    13981 cacgtctcgc acgttttcct acttctttta actttttacc gccttttcca atgacaattc
    14041 ctttttgcga atctctttca acatatatag ttgcttcgat atgaacgcga tcttcgcttt
    14101 ctttaaccat acggtccaca ttaacaccaa tcgcatgagg gatttcttca cttgtaagat
    14161 gaaggatttt ttcacgaatg atttcaccca ctacaaattg ttcaggatgg tctgaaattt
    14221 gatcatctgg ataatattta ggtccttcgg gtaaatacgt ctttaaaaca tcaataaaat
    14281 gatcgacatt tagcccttct aatgctgaaa taggtacaat ctctgtaaag tccatataac
    14341 tttgatattc ttcaatcttt ggcattaatt catctggatg cactaaatct attttattta
    14401 atactaaaaa tactggtgtc ttaacatttt tcaacatttc tataatatat tcatcgcctc
    14461 gtccaatttc ctcattggca ttaaccataa acatgattgc atctatctca gataatgtat
    14521 ttttagcgac tttcatcata tagtcaccta atttgtgttt aggtttatga atacctggcg
    14581 tatcaatgaa tataatttgc gcgtcatctc ttgtcataac accttgaatt ttatttctag
    14641 ttgtttgagc tttatcggac atgattgcta ttttatggcc gatcactcta ttaacaaatg
    14701 ttgactttcc tacatttggt ctacctataa ttgaaacaaa tcctgattta tgttctgtca
    14761 tttattctaa atcctttcct gaaaatccaa atggtagtaa ctctgcgact gtcatcataa
    14821 ccatatctcc tttatgattt gtcatataca caggcatatc atcatcacat aattccttca
    14881 aaacttgacg acatgcacca caaggtgatg acggtttatc tgcatctacg gttacagtta
    14941 ttgattcaaa atcacctggt ctgtatcctt gagaaattgc cgataccaaa ctagctcgtt
    15001 cagcacatat cgataatgga taagaagcat tttctacatt ggtaccataa aaagttctac
    15061 cgtctttcgt ttttaaataa gcccctactt taaattgact gtatggcgaa tatgattctt
    15121 gttgtgcttt tctaacttct tgaaaataat gaggttgata actcatatat gcctccctaa
    15181 aataacgcta taaaatgtgg taaaaatact attaaaccta taataaatgc taatattgaa
    15241 actataagta cactaaaagc cgcaatatct ttagcgtatt tagctaaatc atgatattca
    15301 acggtcacta aatcgacaac atattcaata gcagtgttta aagcttcaac agtgagaact
    15361 aatgcaatag caatgagtat aaatatccac tcaatccgat taatatttag tacgagacca
    15421 aagacaatag caacaatcat tgcaaacaca tgtaaaagaa atttatagtc tttttgaatt
    15481 aagattttca gcccatcaag tgcatattta aaccttttca taattagtct cgtgttaatc
    15541 catatgcgtt taatattgta tcttgtcgac caaacatttc cttttcatcc gcttcagtca
    15601 tatgatcata acctaataga tgcaaaaatc catgtaatgc taaaaatcct aattctcgtt
    15661 caaaagaatg tccgtaattg tttgcttgtt cttgcgctac atccgtacag ataattatat
    15721 cccctaaaac acgtggtata tcaagatcac taaaatcaat ctctggctca tcttcttcta
    15781 aagcaaatga gattacatct gtaactttat ctttatctct atatgttcga ttaatttctt
    15841 gtatttcttg tttatctaca aatgtaacag aaagctcagc atcgtcttct atatgctctt
    15901 cttttttagc aaattctaat aaatcttcaa tttgtttata ccaagcatct ttaactaagc
    15961 ctgtgtgatc gctaaaatct atcgtaaaca tttaattctc tccttcataa tgttcaatga
    16021 tcttacttac caatggatgt ctcactacat cgctctgatc taatttcaat atacttatac
    16081 ctttaacgtt gtgtaaccta ctgaccgctt ctttaagtcc acttttaaca cctttaggta
    16141 aatcgatttg agtttggtca ccagtaacta ccatttttga gccaaaacct agtcttgtta
    16201 aaaacatttt catttgcgca tgtgtcgtat tctgcgcctc atcaagaatt acaaatgcat
    16261 cttctaatgt tcgtccgcgc atatatgcaa gtggcgctat ttcgataatg cctctttcaa
    16321 taaatcgctc tgtttgttca cgcccaagaa cagtatagag accatcatat aaaggtctta
    16381 aatatggatc taccttttct ttcaaatctc ctggtaaaaa tccaagtgac tctcctgctt
    16441 caacagcagg tcttgttaat acaatacgtt taacagcacc tttacggagt tgctttgccg
    16501 cataaactac agctaagaat gtcttacctg taccagcagg acctatacca aatactaaat
    16561 cattattttt catggcatta acatatatac gttgccccat cgttttcgca cgaatcgttt
    16621 taccaaatgc atctttagtt atctcttcat catataaatc taacagatgt tgaattgtgt
    16681 tattatgcgc cattttaata gctgcttcaa catctttaat tgtaatatta ttacctaaat
    16741 caataacctt cagcaaattg attaatactg attccgcttt ttctacgttt tctatttttg
    16801 tacctttaac ggcaacttct tgtcctcttg catggatgac aacatcgaaa ctctcttcaa
    16861 ttgcttttaa atgttcatca ttatttccaa ttaaagcttg agattggttc atatcgtcta
    16921 tttgtataat tccaggcata cacgcgctcc ttttcatata tcagttatat tcattctatg
    16981 atgctttaat atctacttaa ttatatcatg caattgattg atttaaacat ctttgcattg
    17041 acttactcat ttttctttaa tttaaaataa tcatttatac ttttcacctt gtcataaaag
    17101 cagattcaat attagactaa tcaatttttt gttgtaattt aaataactaa tatttctcga
    17161 aagattctct ttgtttttca atttttcata acgtaaaa
//
LOCUS       DAHHOJ010000054        16782 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.054, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000054 DAHHOJ010000000
VERSION     DAHHOJ010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16782
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.054"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            18..644
                     /locus_tag="NBH32_001540"
     CDS             18..644
                     /locus_tag="NBH32_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCD1515136.1"
                     /translation="MAKVLYITAHPFNELVSNSMAAGKAFIETYQQQHPDDEVKHIDL
                     FETYIPVIDKDVLTGWGKMSNGETLTDDEQMKVSRLSDILEEFLSADKYVFVTPMWNL
                     SFPPVVKAYIDAISIAGKTFKYSAEGPQGLLTDKKVLHIQSRGGYYTEGPAADFEMGD
                     RYLRTIMTFLGVPSYETIIIEGHNAEPHKTEEIKATSINNAEKLATTF"
     gene            834..1607
                     /locus_tag="NBH32_001541"
     CDS             834..1607
                     /locus_tag="NBH32_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1515137.1"
                     /translation="MKAHKIFWLNLAAIIIISIVVSGGMFLAMKWEQIHLKDGLKKVL
                     STYPIKNLETLYEIDGHDNPHYENNDQDTWYIESSYSVVGSDELFKEDRMLLKVDKNT
                     HKITGEYDTTTNDRKNATDSTYKSYPVKVVNNKIVFTKDVKDPTLKQKIENNQFLIQN
                     GDLTSILNSNDLKVTHDPTTDYYNLSGKLSNDNPNVKQLKRRYNIPRNASTKVELKGM
                     SDLKGNNHQDQKLYFYFSSPGKNQIIYTESLTYNKLSEH"
     gene            complement(1920..3926)
                     /gene="ggt"
                     /locus_tag="NBH32_001542"
     CDS             complement(1920..3926)
                     /gene="ggt"
                     /locus_tag="NBH32_001542"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCD1515138.1"
                     /translation="MVINLNDKQTKTSKEGLISVSHPLAAKIGKDVLDQGGNAMDAVI
                     AIQLALNVVEPFASGIGGGGYLLYYEQSTGSITAFDARETAPAHVDKQFYLDDSGEYK
                     SFFDMTTHGKTVAVPAIPKLFDYIHKRYAKLSLEDLINPAIELAIEGHSANWATEKYS
                     RQQHARLTKYHETAQVFTHENQYWREGDWIVQPELGKTFQILREQGFNAFYKGDIAKQ
                     LVNVVKACGGTITLEDLAKYDIQIKAPISATFKDYDIYSMGPSSSGGITVIQILKLLE
                     HVDLPSMGPRSVDYLHHLIQAMHLAYSDRAQYLADDNFHEVPVQSLIDDDYLKARSTL
                     IDSNKANIDIEHGVVSDCISHTDVEENHTETTHFCVIDKEGNIASFTTSIGMIYGSGI
                     TIPGYGVLLNTTMDGFDVVDGGINEIAPYKRPLSNMAPTIVMYHGKPILTVGAPGAIS
                     IIASVAQTLINVLVFGMDIQQAIDEPRVYSSHPNRIEWEPQFSQSTILALIARGHAME
                     HKPDAYIGDVHGLQVDLNTRDASGGADDTREGTVIGGDVLSIRKEPLPSPKIYDNDTH
                     RVYFNDMQLPLYAEQVRWMYDKYWVDESVIRIIFPEVSVHIEDLRSYEIAGKNYIDIA
                     WLARKKGYQVTLKDDSLYLTDETYHSVKANTNAYYRYDRDSITR"
     gene            complement(3964..5739)
                     /locus_tag="NBH32_001543"
     CDS             complement(3964..5739)
                     /locus_tag="NBH32_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1515139.1"
                     /translation="MKKIISIAIIVLALVLSGCGVPTKSEVAQKSSKVEVKGERPTIH
                     FLGQASYENDMNIVKDQLENAGFNVKMNIQPDYGSYRTQRQAGNYDIQIDDWMTVFGD
                     PNYAMTALFSSTGSNSLLKDKHVDQLLNKASTQNEADVKQTYKQIEDEVVFDKGYMAP
                     LYGSKKNLVYDNKVLDKNSVGLPNSRALIWQQFDYNNSRERDTRPLVMTQQDGEIPTL
                     DPIRSIAPSVYSINMNMYTRLLLLDENDHLTTKGSLSRDYAVNKDNKAFYFLLRDDDY
                     FAKVVNGQARNTGERVSAEDVKFSLDRARDKKSVPNNNTYNMHKHINDIKILKDEDID
                     QLRKEKDKDDKSIYDKLIKAYNVKSLTTDGQKVNNKDGIYQIVKITTDQSMPREVNYL
                     THSSAGILSKKFVNQVNQEYPKGYGDSSTIPANSDGKNALYASGAYIMTQKNAYQATF
                     QRNPGFNETEKGSYGPAKIKNITLKFNGDPNNALSELRNHSIDMLADVNQKHFDLIKS
                     DKNLSIIRKNGRKSVFLMLNIKKGIFKTHPNLRQAVVNAINQDQFIKFYRGDKFKIAS
                     PITPLVDTGNEQRQDLEKVEKAINQ"
     gene            complement(5756..6919)
                     /locus_tag="NBH32_001544"
     CDS             complement(5756..6919)
                     /locus_tag="NBH32_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515140.1"
                     /translation="MVKLTTKIASLKLFASYAIATYILVILTSALNLFKGYVADTFYI
                     AETLLIVLTIILIIILTTEQTWKHHDLWRRIVEVLLLLMTLTGNVFTLLMFVSIRRYE
                     RTSQIHSYNGWESFIRKTTRHRIAIIGLLILVYMLTLSIVSQFTFDTTLATKNQFNAL
                     LHGPSLAYPFGTDDFGRDLFTRVVVGTKLTFSISIISVVIAVIFGVLLGTIAGYFNHI
                     DNLIMRILDVVFAIPSLLLAVAIIASFGASIPNLIIALSIGNIPSFARTMRASVLEIK
                     RMEYVDAARITGENTWNIIWRYILPNAIAPMIVRFSLNIGVVVLTTSSLSFLGLGVAP
                     DVAEWGNILRTGSNYLETHSNLAIVPGVCIMFVVLAFNFIGDAVRDALDPRIH"
     gene            complement(6925..8403)
                     /locus_tag="NBH32_001545"
     CDS             complement(6925..8403)
                     /locus_tag="NBH32_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515141.1"
                     /translation="MNCTFRLLTSNKFDMMLCLPIHKCVNLFYTFVYHSNLLKIILQG
                     VIIIITYLESFKSLYHKAFYKFVLSVLSLPIFLYAVIKFFFSAKRKNFYANNSEISEI
                     EQALHQKYKYLSQQKLSTQIHKEALKIFKAQSSNTNSKNIEQAHFSTYFENVLFHKFI
                     IIKVILALPMFILLTIYLQPLVRYIFERIVMAVIVIIGVIVSVFTILYFSPLDAAYSI
                     LGQNATKAQIHQFNVLHHLNEPYFIQLWDTIKGVFTFDLGTTYKGNEVVTKAVGERIP
                     ITIIVAVLALIVALIIAIPIGIISAMKRNSWLDITLMIIALIGLSIPSFWQGLLFILA
                     FSLKLDILPPSYMPEHPISLILPVLVIGTSIAASITRMTRSSVLEVMRSDYVLTAYAK
                     GLSTTQVVIKHILKNAIIPIVTLVGLLVAELLGGSAVTEQVFNINGIGRYIVQKQLIP
                     DIPAVMGGVVYISIVISLANLIIDIFYALIDPKLRSEINERK"
     gene            8360..9952
                     /locus_tag="NBH32_001546"
     CDS             8360..9952
                     /locus_tag="NBH32_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515142.1"
                     /translation="MSNLLEVNSLNVQFNYDETTVQAVKNVSFELRKKHILGIVGESG
                     SGKSITAKSILGLLPDYPDHTLTGEIIFNGQSLTNLSTSALQQIRGKDISMIFQDPLS
                     SLNPRLTIGKQITEVLFQHKRVSKSEAKSMTIDILEKVGIKHATRQFDAYPHELSGGM
                     RQRVMIAMALILKPQILIADEPTTALDASTQNQLLQLMKSLYEYTETSIIFITHDLGA
                     VYQFCDDVIVMKDGSVVESGTVESIFKSPQHTYTKRLIDAIPDIHQTRPPRPLNNDIL
                     LKFDRVSVDYKLPSGSLYRAVNDINLAIRKGETLGIVGESGSGKSTLAKTVVGLKEVS
                     EGFIWYNELPLSLFKDDELKALRQEIQMIFQDPFASINPRFKVIDVIKRPLIIHGKVK
                     DNDDIIEIIVSLLEKVGLDQSFLYRYPHELSGGQRQRVSIARALAVESKVIVCDEAVS
                     ALDVSIQKDIIELLKQLQLDFGITYLFITHDMGVINEICDRVAVMKNGEIVELNNTED
                     IIKHPQSDYAKQLISEVAVIAK"
     gene            complement(10217..11329)
                     /locus_tag="NBH32_001547"
     CDS             complement(10217..11329)
                     /locus_tag="NBH32_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HCD1515143.1"
                     /translation="MKICEKCFNNTEIVEIIANDNSKIDNCDIDNNHLGVKIFDTTKD
                     KDKLELIRDYLRPALELYDISINLPDTFSPKEGKKIEIALKDDWSIFKVEEAQISCIL
                     NELFKDDENLDRRVLEDLVGAKIINDKKYTNENLIVANNDWDGFCESLKYNNRFHNNM
                     INLENLAFFLDITTNYYSIEEFRERFEPLYRSRIVKFITEKSELMSPPKGFATAGRLN
                     SKWISVLYLSTKEQVSIEEVKPKHNDIIYIGKVKLQKNVTKEKLKIANLTNLTSNAIK
                     AGDDGFRKYFVNYQTLKKIHKGITNPSDEQGIDYLPFQYLADYIRSLKYDGIMYESIL
                     QDGTFNFVFFDQSLFECVNYERKRVSDVKYTLTKLS"
     gene            complement(11316..12239)
                     /locus_tag="NBH32_001548"
     CDS             complement(11316..12239)
                     /locus_tag="NBH32_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HCD1515144.1"
                     /translation="MYYPYLRGKQNELFAIKELLEKGLIGDCIQPIIEPIKYTTTFKN
                     TLQYCGEKAFSINLVVNSKLTEEEISNETVEHLTEIITKNKSVIQKAYLGPSDEGNDR
                     LKQQFSSNSLAILTSVDDWEMFGDKNKLEMVFIPDDRHIKRKLRNIPNKGIIMDPFNK
                     LSRNVDYLDNDDEFYSDDHLYYKEDGYVAFSDYSVIGGEYVDGGFSPLAIAIHIVYFD
                     EANELRVKHFVSDSNNDRSNPGKKFFEAVDKLVTWSKNLDIKNRSYALGQFEELNENN
                     KYPGLGLIKRLSIMHHLEIMNRYLESQNENM"
     gene            complement(12241..13083)
                     /locus_tag="NBH32_001549"
     CDS             complement(12241..13083)
                     /locus_tag="NBH32_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7726 family protein"
                     /protein_id="HCD1515145.1"
                     /translation="MDNRNMINRVFSQKILHQIAIKNKSDVVDEAYDFYIQGPKNINV
                     IQKMKSLYNYLKKSYRNEYFYKNTMLNKLLLGRHSVNTTTALSEMPIGKSIADFILLN
                     GKGVVYEIKTELDKLDRLDNQINDYYEVFNYVVVITNNKHLNKVMARYKDTTVGILVL
                     TTRNTLSEVQKPKENNSLLNTKAMYNFLRKEERKRVIAQNHMDVPTYNDFTEYDVLFD
                     VFKEIPMTKLHNNMISELKKRGNMKEYKDEFLAAPTEIKFLLYFAKMTKKDKNKLYHF
                     LKED"
     gene            complement(13283..16072)
                     /locus_tag="NBH32_001550"
     CDS             complement(13283..16072)
                     /locus_tag="NBH32_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD1515146.1"
                     /translation="MAYQSEYALENEMMNQLEQLGYERVTIRDNKQLLDNFRTILNER
                     HADKLEGNPLTDKEFQRLLTMIDGKSIFESARILRDKLPLRRDDESEIYLSFLDTKSW
                     CKNKFQVTNQVSVEDTYKARYDVTILINGLPLVQVELKRRGIDINEAFNQVKRYRKQN
                     YTGLFRYIQMFIISNGVETRYFSNNDSELLKSHMFYWSDKQNNRINTLQSFAESFMRP
                     CQLAKMISRYMIINETDRILMAMRPYQVYAVEALIQQATETGNNGYIWHTTGSGKTLT
                     SFKASQILSQQDDIKKVIFLVDRKDLDSQTEEEFNKFAKGAVDKTFNTSQLVRQLNDK
                     SLPLIVTTIQKMAKAIQGNAHLLEQYKTNKVVFIIDECHRSQFGDMHRLVKQHFKNAQ
                     YFGFTGTPRFPENSSQDGRTTADIFGRCLHTYLIRDAIHDGNVLGFSVDYINTFKNKA
                     LKAEDNSMVEAIDTEEVWLADKRVELVTRHIINNHDKYTRNRQYSSIFTVQSIHALIK
                     YYETFKRLNKKLEQPLTVAGIFTFKPNEDDRDGEVPYHSREKLEIMISDYNKKFETNF
                     STDTTNEYFNHISKNVKKGVKDSKIDILIVVNMFLTGFDSKVLNTLYVDKNLMYHDLI
                     QAYSRTNRVEKESKAFGKIVNYRDLKKETDDALRVFSQTNDTDTILMRSYEEYKKEFM
                     DAYRELKMIVPTPHMVDDIQDEEELKRFVEAYRLLAKIILRLKAFDEFEFTIDEIGMD
                     EQENEDYKSKYLAVYDQVKRATAEKNKVSILNDIDFEIEMMRNDTINVNYIMNILRQI
                     DLEDKAEQRRNQEQIRRILDHADDPTLRLKRDLIREFIDNVVPSLNKDDDIDQEYVNF
                     ESIKKEAEFKGFAGERSIDEQALKTISNDYQNSGVVNPHHLKKMIGDLPLKEKRKARK
                     AIESFVAETTEKYGV"
BASE COUNT         5427 a         3039 c         2416 g         5900 t
ORIGIN      
        1 cagaggagaa ttttcaaatg gcaaaagtat tatatattac agcacaccct tttaatgaac
       61 tggtatcaaa ttctatggcg gctggtaaag catttatcga aacatatcaa caacaacatc
      121 ctgatgatga agtgaaacat attgatttat ttgaaactta tattccagtt attgacaaag
      181 atgtattaac tggttgggga aaaatgagca atggcgaaac gttaactgat gatgaacaaa
      241 tgaaagtttc aagattgagt gacattttag aagaattttt aagcgcagat aaatatgtat
      301 ttgtaacgcc gatgtggaac ctttctttcc cacctgtagt caaagcatat attgatgcca
      361 tttcaattgc aggtaaaact tttaaatatt ctgctgaagg acctcaaggt ttgttaactg
      421 acaaaaaagt attacacatt caatcacgtg gtggatatta tactgaagga cctgctgctg
      481 attttgaaat gggtgaccgt tatttaagaa caattatgac attcttaggt gtgccatcat
      541 atgaaacaat cattattgaa ggacacaatg ctgaacctca taagactgaa gaaattaaag
      601 caactagtat taataatgct gaaaaattag caacaacttt ttaaattttt aattacactg
      661 tgtgactcct caaacgtcac acagttttta attgtatgca tttcctactt ttttataaat
      721 acctattatt tccacacatt taaagtttga tattcacttt aatactgatg taaatatttt
      781 taaaatatat tattatagtg ttaattaagt attttaagaa aaggtgtgaa tgtatgaaag
      841 cacataaaat tttctggtta aacttagcag caattattat tatttcaatt gtagtcagtg
      901 gcggtatgtt tttggcaatg aagtgggaac aaattcattt gaaagatggt cttaaaaaag
      961 tactaagtac atatcctatt aaaaacttag aaacgcttta tgaaattgat ggtcacgata
     1021 atccccatta tgaaaataat gatcaagaca catggtacat agaatcctct tattcagttg
     1081 ttggatcaga tgagcttttt aaagaagacc gtatgttgtt aaaggtagat aagaacacac
     1141 ataaaataac tggtgaatat gacacaacta caaacgatag aaaaaatgct actgactcga
     1201 catacaaaag ttatccagta aaagtagtta ataataaaat cgttttcaca aaagacgtaa
     1261 aagatcccac gctgaaacaa aaaatcgaaa acaatcaatt tttgattcaa aatggtgatt
     1321 taacaagcat tttaaatagc aatgatttga aagtcacaca tgatcctacc actgattatt
     1381 ataatttatc tggtaagttg tcgaatgata atccaaacgt taaacaatta aaacgtagat
     1441 ataatattcc cagaaatgca tcaacaaagg tggaattaaa gggaatgagt gatttaaaag
     1501 gcaataatca tcaagatcag aaactttatt tttatttttc aagtcctgga aaaaaccaaa
     1561 ttatatacac agaaagcctt acttataata aattaagtga acattaatac ttatgatata
     1621 attatagaaa taaccaaatc atttattatc gtttaacata ctaaaatcac acattataaa
     1681 aacgcctcac atcattcatt taattgtacc gaatcacatc aagttagatt ttagactaac
     1741 tattgtggtg tacatcaaaa acgaatttgt ggggcgtttt atattttaca aaggcgacta
     1801 gcgtttctta taacaacttc aacattacta cactcaagca tcactctaaa gtttgaaatc
     1861 gaaccctttc gttcaacaaa aatcaaaatc cacttcaaac tatatatcaa atacataaat
     1921 tatcttgtga tactatctcg atcatatcta tagtatgcat ttgtgtttgc tttcactgaa
     1981 tgatacgttt catcagttaa gtataaactg tcatccttta aggtaacttg ataacctttc
     2041 ttccgtgcca accaggcaat atctatataa ttttttccgg cgatttcata acttcttaaa
     2101 tcttcaatat gcacactaac ttcagggaaa atgattctaa taacactttc atcaacccaa
     2161 tatttgtcat acatccaccg cacttgttca gcatataatg gcaactgcat atcattgaaa
     2221 tatactcgat gggtgtcatt atcgtatatt ttcggactag gtaatggttc ttttctaatt
     2281 gataatacat cgccacctat cactgtgcct tctctcgtat catcagcacc tcccgacgcg
     2341 tcacgtgtat tcaagtcaac ctgtagtcca tgtacatctc caatataggc atctggttta
     2401 tgttccattg catgtccacg tgcaatcaat gctaatattg tagattgtga aaattgaggc
     2461 tcccattcaa tgcgattagg atggctacta taaactctag gttcatctat agcctgctga
     2521 atatccatgc caaacactaa tacattgatt aatgtttgcg caacactagc aatgatactt
     2581 atggcaccag gtgcacctac tgttaatatc ggcttcccgt gatacatcac aatcgttgga
     2641 gccatgttac ttagtggtcg tttatatggt gcaatttcat taataccacc atctactaca
     2701 tcaaagccat ccattgtcgt attcaataac acaccgtagc ctggaatcgt aatacctgaa
     2761 ccataaatca taccaattga tgtcgtaaat gaagcaatat taccttcctt atcaatcaca
     2821 caaaaatgag ttgtttcggt atgattttct tcaacatctg tatgactaat gcaatcagac
     2881 acaacaccat gctctatatc aatatttgct ttattgctat caatgagcgt actgcgtgct
     2941 tttaaataat cgtcatcaat taatgactgt acaggcacct catgaaaatt atcatccgcc
     3001 aagtattgcg cacgatcact atatgctaaa tgcatcgctt gtatcaaatg atgcaagtaa
     3061 tcaacagatc ttggacccat agatggtaaa tcgacatgtt ctaataactt caatatttga
     3121 attaccgtga taccgccaga actagatggt cccattgaat aaatgtcata gtctttaaat
     3181 gttgcactga ttggcgcttt aatctgaatg tcatatttgg ctagatcctc taaagtgatt
     3241 gtcccaccac atgctttgac aacattgact aattgtttcg caatgtcacc tttataaaat
     3301 gcattaaacc cttgttctct taatatttga aatgtcttac ctaattcggg ttgtacaatc
     3361 caatcacctt cacgccaata ttgattttca tgcgtaaata cttgcgccgt ttcgtgatac
     3421 tttgtcaatc gtgcgtgttg ctggcgcgaa tatttttcag tagcccaatt ggctgaatga
     3481 ccttcaatgg ctagttcaat tgcaggatta attaaatctt ccaatgacaa tttagcataa
     3541 cgcttgtgaa tataatcaaa cagctttgga attgctggca cagcgacagt tttaccatgt
     3601 gtagtcatat caaaaaatga tttatattcg cctgaatcat ctagataaaa ttgtttgtct
     3661 acatgtgcag gggctgtctc acgcgcatca aacgcagtta tactgccagt actttgctca
     3721 taatataaca agtacccgcc accaccaata cctgatgcaa atggttctac cacattcaat
     3781 gccagttgaa ttgcaatcac tgcatccatg gcgttgccac cttgatctaa tacatcctta
     3841 ccaattttag ccgcaagagg atgtgatacg gaaattaacc cttctttaga tgtttttgtc
     3901 tgtttgtcat ttaagttaat gaccattcta tatcctccta ctttctgtta aatatttaaa
     3961 acattattga ttaatggctt tttctacttt ttctaaatct tgacgttgct cgttaccagt
     4021 atcgacaagt ggtgtaatcg gtgatgcaat tttaaattta tcgccacgat aaaacttaat
     4081 aaattgatcc tgatttatcg cattaactac tgcttgtctc aagtttggat gcgtcttaaa
     4141 tatacctttt ttaatattta gcattaaaaa gactgatttg cgtccatttt tgcgaataat
     4201 gcttaaattt ttatccgact taattaaatc aaaatgtttt tgattcacat ctgccaacat
     4261 atcaattgaa tgatttctaa gttctgacaa tgcattattc gggtcaccat taaacttcaa
     4321 tgtaatattt ttaattttag ctggtccata actacctttt tctgtttcgt tgaatcctgg
     4381 attacgttga aacgttgctt gatatgcatt tttctgtgtc ataatgtatg caccacttgc
     4441 ataaagcgca ttttttccat ctgaatttgc aggaattgta ctgctatccc catatccttt
     4501 tggatattct tgatttactt gattaacaaa ttttttagat aaaatgcctg ccgaagagtg
     4561 tgttaagtaa tttacctctc gaggcatcga ttgatctgtc gtaattttaa caatttgata
     4621 aataccgtct ttattattta ctttttgacc atctgtcgtt aacgatttga cgttatacgc
     4681 tttaatcaac ttatcataga ttgatttatc gtccttgtct ttctctttac gcaactgatc
     4741 gatgtcctca tcttttaata tcttgatgtc atttatatgt ttgtgcatat tgtaagtatt
     4801 attgttaggc acagactttt tatcacgtgc tctatctaaa gaaaacttaa catcttcagc
     4861 cgatacacgc tctccagtat tacgtgcttg tccattgacc actttcgcaa aataatcatc
     4921 atctcttaac aagaaataaa acgctttatt gtccttattc acagcataat cacgacttaa
     4981 cgaacctttc gttgttaagt gatcattttc atctaataat aataaccttg tgtacatatt
     5041 catattaatt gaatatactg acggcgcaat tgaacgtatt ggatccaatg taggaatttc
     5101 accatcttgt tgtgtcatca caagtggccg cgtatctcgt tctctactat tgttgtaatc
     5161 aaattgttgc catattaatg cacgtgaatt tggcaatcca acactatttt tatctaacac
     5221 tttattgtca tatactaaat tcttttttga tccatataat ggcgccatat accctttatc
     5281 aaatacaact tcatcttcaa tttgcttata tgtttgttta acatctgctt cattttgagt
     5341 agaagcttta tttaacaact ggtctacatg tttatctttc aataaactat ttgatcctgt
     5401 agaactaaat aatgccgtca tagcatagtt cgggtcacca aaaactgtca tccagtcatc
     5461 gatttggata tcataattgc cggcttgacg ttgtgtacga tagctaccat aatctggttg
     5521 gatattcatc ttcacgttaa atcctgcatt ttccaattga tctttgacga tattcatatc
     5581 attttcataa cttgcttgtc ctaggaaatg tattgttggt cgctcgcctt tcacttcaac
     5641 tttcgatgac ttttgagcca cttctgattt cgtagggaca ccacaaccac ttaataccaa
     5701 cgctaaaact ataattgcga tactaatgat tttcttcaca tctatcccta cctttttaat
     5761 gaattcttgg atctagtgca tcacgcactg catcacctat aaaattaaat gctaaaacga
     5821 cgaacataat acaaacacca ggtacaatag ctaaattact gtgcgtttcc aagtagttac
     5881 taccggtacg taaaatgttg ccccattcag ctacatcagg tgcaacacca agtcctagga
     5941 aacttaaact acttgttgtt aatacaacca cacctatatt taatgaaaaa cgtacaatca
     6001 taggcgcaat cgcattcggt aaaatataac gccatatgat attccaagtg ttttcaccag
     6061 tgatacgcgc tgcatctaca tattccatgc gtttaatttc taaaacactg gcacgcattg
     6121 tccgtgcaaa tgatggtata ttaccgatac ttaaagcaat aattaaattg ggaatacttg
     6181 ctccaaatga tgcaataatt gccaccgcta acaataatga tggaattgca aacactacat
     6241 ctaaaattcg cattattaaa ttatcaatat gattaaaata acctgcgata gtgcctagta
     6301 acacaccaaa aataactgca ataactactg aaataattga aattgaaaat gtcagcttcg
     6361 ttccgacaac tacgcgtgta aataagtctc taccgaaatc atcagtacca aacggatagg
     6421 ctagactcgg tccatgtaac agtgcattga actgattttt agtagccaat gtcgtatcaa
     6481 atgtaaattg tgacacaatt gataatgtca gcatgtagac taaaataagt aacccgataa
     6541 tcgcaatacg atgtctagta gtttttcgta taaacgattc ccagccgtta taactatgta
     6601 tttgcgatgt acgttcataa cgtctaatac ttacaaacat taataatgta aatacgttgc
     6661 ctgttaatgt catcaataat aacaacactt cgacaatacg tcgccatagg tcatgatgct
     6721 tccatgtttg ttccgttgtt aagatgataa ttaaaatgat agttaaaacg attagcaatg
     6781 tttcagcaat atagaacgta tcggccacat aacctttaaa aagatttaat gcactcgtta
     6841 atataactaa aatataagtt gcaatagcat aacttgcgaa tagttttaag gaagctatct
     6901 ttgttgtaag ttttaccata tgcctcactt cctttcgtta atttcactac gtaattttgg
     6961 atcgattaaa gcataaaata tatcaataat taagtttgct aaagatatta caattgatat
     7021 atatacgacc ccacccatga ctgctgggat atcaggtatt agttgttttt ggacgatata
     7081 acgaccgata ccattaatgt taaatacttg ttccgtcact gctgaaccgc ctagtaactc
     7141 tgccactaga agaccaacta gcgttacaat tggaataatg gcatttttca aaatatgttt
     7201 aataactact tgtgtcgtcg ataatccttt tgcataagca gttaaaacat aatcactgcg
     7261 cattacttca agtacagaag accttgtcat acgcgtgata gaagcagcaa tacttgttcc
     7321 aatgacaagt acaggtaaaa tcaacgatat tggatgttct ggcatataag atggtggcaa
     7381 aatatccaat ttcaatgaga acgctaaaat gaataatagc ccttgccaga aacttggaat
     7441 agataaacca attaatgcaa ttatcattaa cgtgatatca agccaactat ttcgcttcat
     7501 cgcactgata attccaattg gtatcgcaat aattaatgcc acaattagcg ctaatactgc
     7561 gacaattatt gtaattggaa ttctttcgcc aactgcttta gtcacaacct cattcccttt
     7621 gtaagtcgta cctaagtcaa aagtaaaaac acccttgatg gtatcccaca attgaataaa
     7681 ataaggttcg ttaagatgat gtaatacatt gaattgatgt atctgtgcct ttgttgcatt
     7741 ttgtcccagt atgctataag ccgcatcaag cggtgaaaaa tacagaatgg taaacacact
     7801 gacaataaca ccaatgatga caatcacagc catgacaatt cgctcaaaaa tatatctaac
     7861 taatggctgt aaataaatag tcaataagat gaacatcggc aaggccaata tcactttgat
     7921 aatgatgaac ttatgaaata atacattttc aaagtatgtt gaaaaatgtg cttgttcaat
     7981 attctttgaa ttcgtattag aactttgtgc cttgaatatt tttaatgctt ctttatgtat
     8041 ttgtgtggat aacttttgct gcgataaata tttatatttt tgatgtaacg cctgttcgat
     8101 ttctgaaatt tcagaattat tagcgtaaaa atttttcctc ttagcagaaa agaaaaactt
     8161 tatcactgca tataaaaata ttggcaagct taataccgat aatacaaact tgtaaaatgc
     8221 cttgtgatac aacgacttaa aagattctaa ataagtaatt atgataatca ctccttgtaa
     8281 aataatcttg agtagattac tatgatatac aaaagtatag aataaattta cacatttgtg
     8341 aatagggagg cacaacatca tgtcaaattt attagaagtc aacagtctga atgtacaatt
     8401 caattatgat gaaactacag ttcaagcggt aaaaaacgtc tctttcgaat tacgaaaaaa
     8461 acatatccta ggtattgttg gtgaatcagg atcaggaaaa agtattactg ctaaatctat
     8521 tttagggcta ctaccagatt atccagatca cacattaaca ggagaaatta tttttaatgg
     8581 gcaatcgtta actaatttat caacttcagc gttacaacaa attcgaggca aggatatttc
     8641 aatgattttt caagatccac tctcttcgtt gaatccaaga ttaacgattg gcaaacaaat
     8701 tacagaagta ctatttcaac ataaacgtgt atctaaatct gaagcaaagt cgatgacaat
     8761 agacatttta gaaaaagtag gtataaaaca tgcaactcga caatttgatg cttatccaca
     8821 tgaactttct ggtggtatgc gtcaacgtgt catgatagca atggcattga ttttaaagcc
     8881 acaaatttta atcgcagatg aaccaacaac ggcattagat gccagtacac aaaatcaatt
     8941 actgcagtta atgaagtccc tttatgagta cacagaaaca tctattattt ttatcactca
     9001 cgatttaggc gctgtgtatc aattttgcga cgatgtgatt gtaatgaaag atggaagtgt
     9061 cgttgaaagt ggcacggttg aaagtatttt taaatcgcca caacatacct atacgaaacg
     9121 cttaatagat gcgattccag atattcatca aacgcgtccg ccaagaccgt taaacaatga
     9181 tattttatta aaattcgatc gcgtgagcgt ggattacaag ttaccaagtg gcagcctata
     9241 ccgagcagtt aatgatatta acttggctat tagaaaaggc gaaacattag gcattgtcgg
     9301 tgagtcaggg tcagggaaat cgacattagc taagacggtc gtcggtctaa aggaagtgtc
     9361 agaaggcttt atttggtata acgaattacc attaagttta tttaaagatg atgaattgaa
     9421 agctttacga caagagatac aaatgatttt ccaagatcca ttcgcatcta ttaatccaag
     9481 atttaaagtc attgatgtga ttaaacgacc actaatcatt cacgggaaag tcaaagataa
     9541 tgatgatatt attgaaatta tcgtatcgtt gttagaaaaa gttggcctag atcaaagttt
     9601 cttatatcgc tatccacacg aattatctgg tgggcaacgc cagcgtgtaa gtatcgcgag
     9661 agcacttgct gtagaatcta aagtgattgt ttgcgacgag gcagtgtccg ctttagacgt
     9721 ttcaattcaa aaagatatca tcgagttatt aaaacaatta cagttagact tcggcattac
     9781 ttatttattc atcacacatg acatgggtgt tatcaatgaa atatgtgatc gcgttgcagt
     9841 tatgaaaaat ggcgaaatcg ttgaacttaa taacacagaa gatattatca aacatccgca
     9901 gtcagactat gcaaagcaac ttatttcaga agtagcagtt attgctaaat aaaagtcatg
     9961 cgttgtgtaa ctttatcact gtatggtctg gaataaatca ggcaatctct gatgtgttat
    10021 gttagatgac tttgtcatac taactatatt gtgttaagtt agggctcggc accatatcaa
    10081 gttttaggtg ctttgccatg atttaagagt cacccccata ctttgggcat tttgacgcca
    10141 gaataaatcc cccgctacta tgtgaagtgc gggggattat ttatatttta ttagaatatt
    10201 cagatttttg agtgtgtcaa cttagcttag tcaatgtata tttaacatca cttactcttt
    10261 ttctttcata attaacacat tcaaataaac tttgatcaaa aaacacaaag ttaaaagtac
    10321 catcttgcaa tatgctctca tacattatcc cgtcatattt aaggcttcga atataatcag
    10381 ctaaatattg aaatggcaaa taatctattc cttgttcatc gcttggattt gttattcctt
    10441 tatgaatctt ttttaatgtt tggtaattta caaaatactt tctaaatcca tcatcgccag
    10501 ctttgattgc attactagtt aaattagtta aattcgcaat tttcaatttc tcttttgtaa
    10561 cgtttttttg taacttaacc ttacctatat aaataatgtc attatgctta ggtttaactt
    10621 cttctatact gacctgttct tttgtactaa ggtataatac gcttatccat ttagaattca
    10681 atcttcctgc cgttgcaaat ccctttggtg gtgacattag ttcacttttc tctgtaatga
    10741 acttaactat tctagatcta tataatggtt caaatctttc tctaaattcc tcaatactat
    10801 agtaattagt agtgatatcg agaaagaacg ctaaattctc taaattaatc atattattat
    10861 gaaatctatt gttatacttc aagctctcac aaaatccatc ccagtcatta tttgctacaa
    10921 ttagattttc atttgtatat tttttatcgt ttatgatttt agcgcctact aaatcttcca
    10981 acactcgtct atctaaattt tcatcatctt taaaaagttc atttaaaata caacttattt
    11041 gagcttcctc aactttaaat atactccagt cgtcttttaa tgctatttca atctttttac
    11101 cttcttttgg gctaaaagta tctggtaaat ttatactaat atcatataat tctaatgctg
    11161 gtcttaaata atctctaata agttctaatt tatctttgtc cttagtcgta tcaaatattt
    11221 taacaccaag atgattgtta tcaatatcac aattgtcaat tttgctatta tcatttgcaa
    11281 tgatttctac gatttcagta ttattaaaac atttttcaca tattttcatt ttgagactcc
    11341 aagtatctat tcataatttc tagatgatgc atgatagata accttttaat taaacctaag
    11401 cctggatact tattattttc atttaattct tcaaattgtc caagcgcata agatctattt
    11461 ttaatatcta agttttttga ccatgttact aatttatcta cagcctcaaa aaacttttta
    11521 cctggatttg atctatcatt attagaatca gagacaaaat gcttaactct tagctcatta
    11581 gcctcatcaa aatagacaat atgtatcgca atagctaatg gcgaaaagcc accgtctaca
    11641 tattctccac ctataacaga atagtctgaa aatgctacgt atccatcttc cttataataa
    11701 aggtgatcgt cgctataaaa ctcgtcatca ttatctaaat aatcaacatt acgacttagt
    11761 ttattaaaag gatccataat gatgcctttg tttggaatat tacgcaattt acgtttaatg
    11821 tgtctatcat ctggtataaa aaccatttca agtttatttt tatctccaaa catttcccag
    11881 tcatctacac ttgttaaaat agctaaacta ttacttgaaa attgctgttt caacctatca
    11941 ttgccttcat cagaaggacc caagtaagct ttttgaataa cacttttgtt ttttgttatt
    12001 atttcagtta aatgttcaac agtttcgtta ctaatctctt cttcagttaa ctttgaattt
    12061 actactaaat ttatagagaa tgctttttca ccacagtatt gcaaagtatt tttaaatgtg
    12121 gttgtatatt taattggttc aattataggt tgaatacaat caccaatcaa acctttctct
    12181 aacaattctt taattgcaaa tagttcattt tgtttcccac gcaaataagg ataatacata
    12241 ttaatcctcc ttaagaaaat gatatagttt atttttatct ttctttgtca tttttgcgaa
    12301 atataacaag aacttaattt cagtcggagc tgctaaaaat tcatctttgt attctttcat
    12361 gttgcctctt tttttcaact cagaaatcat attgttatgc agtttcgtca ttggtatttc
    12421 tttaaacacg tcaaataaca catcatactc tgtgaaatca ttataagttg gtacatccat
    12481 atgattttgt gcaataactc tttttctttc ttcttttcgt aaaaagttgt acatcgcttt
    12541 cgtgtttaag agactattgt tttctttagg tttttgaact tcactcagtg tatttctagt
    12601 tgttaacact aaaattccaa ctgttgtatc tttgtatcta gccataactt tattcagatg
    12661 tttgttattt gtaatgacta cgacataatt aaacacttca taataatcat taatttgatt
    12721 atctaatcta tccaacttat ctaattctgt tttaatctca tagacaacgc ctttgccgtt
    12781 taacaatata aaatcagcaa tacttttccc tatgggcatc tcagaaagtg cagtagttgt
    12841 attaacagaa tgtcgtccta gaaggagttt attaagcatt gtgtttttgt aaaaatattc
    12901 gttacgataa gactttttaa gatagttata taaagacttc attttctgta ttacattgat
    12961 atttttaggt ccctgtatat aaaaatcata tgcctcatca acaacatcac ttttattttt
    13021 gattgcaatt tgatgtaata tcttttgact aaaaacacga ttaatcatat ttctattatc
    13081 catagctacg cacctccttc aacatacatt taatacttat cctgttaata ttataaacta
    13141 ttttaaatgc ctatagacat tatttctata cacatacgat gataaaattt tattgaaacc
    13201 aagtgatgat tgcaaaataa atgagccttt tctaatgaaa taattatgcc ccctacacta
    13261 atctagcgag ggggctgaat cattacacac cgtatttttc agttgtttct gccacgaaag
    13321 attcaatggc ttttcttgct ttacgttttt cttttaatgg caaatcacca atcatttttt
    13381 taaggtgatg tgggtttaca acaccactat tctgatagtc atttgaaatt gtttttaggg
    13441 cttgttcatc gatagatctc tctccagcaa atcctttgaa ctccgcttct tttttaatac
    13501 tttcgaaatt aacatattct tgatcgatat catcatcctt atttaaagaa ggtacaacat
    13561 tgtcgatgaa ttctctaatt agatctcgtt ttaacctcaa tgtcggatca tctgcatgat
    13621 ctaaaatgcg tctaatttgt tcttggttac gacgttgttc cgctttgtct tcaagatcaa
    13681 tttgtctcaa tatattcata atataattca cattaatcgt atcattacgc atcatttcta
    13741 tttcgaaatc aatatcattt aaaatggata ctttattttt ctcagccgtc gctcttttta
    13801 cttgatcgta cacagctaaa tatttacttt tatagtcttc attctcttgt tcatccattc
    13861 caatttcatc aattgtaaac tcaaactcgt caaatgcttt taaacgtaat attattttag
    13921 ctaataaacg ataagcttca acaaagcgct ttagctcttc ttcatcttga atgtcatcaa
    13981 ccatgtgtgg tgtcggcaca atcattttaa gctcacgata agcgtccata aattcttttt
    14041 tatactcttc ataactacgc attaaaattg tatccgtatc atttgtttgt gagaatactc
    14101 tcagtgcatc gtctgtctct tttttcaagt cacgatagtt tacaatttta ccaaatgcct
    14161 ttgattcttt ttcaacccta tttgtacgtg aatacgcttg aattaaatca tgatacatta
    14221 aattcttatc aacatataaa gtgttcagta ctttactatc aaaaccagtt aagaacatat
    14281 taacaacgat taagatatca attttactat ctttaacgcc ctttttaacg ttttttgaaa
    14341 tatgattaaa atactcatta gttgtgtctg ttgaaaaatt cgtctcgaac tttttattat
    14401 aatcgctaat cattatctct aatttttcac gtgaatgata tggcacttca ccatcacgat
    14461 catcttcatt aggtttaaac gtaaatatac cagctaccgt taatggttgt tccaactttt
    14521 tgttaagtcg cttaaatgtc tcataatatt taataagcgc gtgaatactt tggactgtaa
    14581 atatacttga atattgacga ttacgtgtat atttatcatg attattgatg atatgtcgtg
    14641 ttactaattc cacacgttta tccgctaacc atacttcttc cgtatcaatt gcttcaacca
    14701 tgctgttatc ttctgctttt aaagctttat ttttaaaagt attaatatag tcaactgaga
    14761 aaccaagtac attaccatca tgaatggcat ctctaattaa atacgtatgt aagcatctac
    14821 cgaaaatatc tgcagttgtt ctaccatctt gactactatt ttctggaaaa cgtggcgtac
    14881 cagtgaaccc aaagtattgg gcatttttga aatgttgttt aactagacga tgcatgtcac
    14941 caaattgact gcgatgacac tcatcaataa taaatacaac tttattcgtt ttatactgtt
    15001 ctaataaatg ggcattacct tgaatcgctt tagccatttt ttgaatcgtc gttacaataa
    15061 gtggcaaact tttatcattt agttggcgta ccagttgcga ggtattaaaa gttttgtcta
    15121 cagcaccctt agcaaattta ttaaattcct cttctgtttg actatccaag tctttgcggt
    15181 caaccaaaaa gataactttc ttaatgtcat cttgctgtga taaaatctga ctcgctttaa
    15241 aagaagtcaa cgtcttacca cttccagttg tatgccaaat atatccatta ttccctgtct
    15301 cagtcgcttg ttgaattagt gcttctaccg catacacttg atacgggcgc attgccatca
    15361 gtattctatc tgtttcatta ataatcatat agcgcgatat catcttagct aattgacaag
    15421 gtctcataaa tgactcagca aacgattgta atgtattgat tcggttattc tgtttatcac
    15481 tccaataaaa catgtgactc ttcaatagtt cgctatcatt attagaaaag tatcgcgttt
    15541 caacaccatt actaatgata aacatttgta tgtagcggaa taagcctgtg taattttgtt
    15601 tacggtaacg ttttacttgg ttaaacgcct cattaatatc aatgcctcga cgtttcaatt
    15661 caacttggac aaggggtagt ccattgatta atatcgttac atcataacgt gctttatatg
    15721 tatcctcgac agatacttga ttcgtcactt gaaacttatt tttacaccaa cttttcgtat
    15781 ctaaaaacga caaataaatc tcagactcat catcacgtct aagtggtaat ttatcacgta
    15841 aaatacgggc actctcgaaa atacttttcc catcaatcat cgttaacaga cgttgaaatt
    15901 ctttatctgt taagggattg ccttctaatt tgtccgcatg acgctcattt aaaatcgttc
    15961 taaaattatc aagcaattgc ttattatcac gtatcgttac tctttcgtaa cccaattgtt
    16021 caagttgatt catcatttca ttttctaatg cgtattcact ttggtatgcc attcatatcc
    16081 ccttccatac actttctatt gctctaaata tatcataaac tttaatgaaa aatgtttgtt
    16141 ttttatcttc aaacgtaaat ttattctaat tttattgtct tatcttttaa tatttgtctt
    16201 tgaggtaagt cgtatactaa aaacaccaac acacactatc ctttatattt ttatacttct
    16261 atgccagagt tcaacttttg aatatattgt gtcctcaatt gtttgttctc tgtatatatt
    16321 ttattgaata tgattgattg aaaaacaaaa gtgaaaatgt acatcacaat tggaataaaa
    16381 gcattataaa tgccattatc aaaataggat aaagaaaaaa taaataaagt gaacactatc
    16441 ggcattgtaa aacgacataa aataatagac caattattta taaaaagcca acttcttatt
    16501 aagttttttc tattcagcgt aatgatatgg aatattttgt cgtttttatt ttgataaaaa
    16561 ctttcaactt tttcatagtg tccatatatg gtttgattta ggttaattat tttgtagttt
    16621 gatccaggtt cgtaaaggtc attattgcca actttaaaac aatctttaat taatgtactt
    16681 tctttgttta caacaaacct atagggatgt atgtaatcac aattttcaca cattttatat
    16741 aattgaaatc tattaacaat cgctaaataa tttctgtaag tt
//
LOCUS       DAHHOJ010000055        16664 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.055, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000055 DAHHOJ010000000
VERSION     DAHHOJ010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16664
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.055"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            316..1539
                     /locus_tag="NBH32_001551"
     CDS             316..1539
                     /locus_tag="NBH32_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HCD1515147.1"
                     /translation="MTTFSEKEKIQLLADIVELQTENNNEIDVCNYLTDLFDKYDIKS
                     EILKVNEHRANIVAEIGNGSPILALSGHMDVVDAGNQDNWTYPPFQLTEKAGKLYGRG
                     TTDMKGGLMALVITLIELKEQNQLPQGTIRLLATAGEEKEQEGAKLLADKGYLDDVDG
                     LIIAEPTGSGIYYAHKGSMSCKVTATGKAVHSSVPFIGDNAIDTLLEFYNQFKEKYSE
                     LKKHDTKHELDVAPMFKSLIGKEISEEDANYASGLTAVCSIINGGKQFNSVPDEASLE
                     FNVRPVPEYDNDFIESFFQNIINDVDSNKLSLDIPSNHRPVTSDKNSKLITTIKDVAS
                     SYVEQDDIFVSALVGATDASSFLGDNKDNVDLAIFGPGNPLMAHQIDEYIEKDMYLKY
                     IDIFKEASIQYLKEK"
     gene            complement(1911..2756)
                     /locus_tag="NBH32_001552"
     CDS             complement(1911..2756)
                     /locus_tag="NBH32_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1515148.1"
                     /translation="MKQLVGIPESMLIPLIARAKEYENEKPIIKDALSKKIFDGLDDM
                     YKNVTCDDMSQIGISIRTVIIDCVTKRLIKDNKDLIVVNIGCGLDTRFQRFNKEKISW
                     IDLDVPESIEIRKTFFKESNSYKMISKSMLDYSWIDDVKNYKFFNSKSDILFIIEGVL
                     MYFDESVMTQLLDTIIKKMGDHNLTFAIEFCSKTIANNTKRHQSVSKLSSQPVFKYGY
                     NDLKKLNEILPNTIRVIHEYNYFDYYKNRWGLFGYCRFIPYLKKRLNNKIVLMKYKVP
                     KRQRH"
     gene            complement(2818..3729)
                     /locus_tag="NBH32_001553"
     CDS             complement(2818..3729)
                     /locus_tag="NBH32_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-hydroxamate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1515149.1"
                     /translation="MKRLIGILLCNLFILTACSASVDKTSNSTKTTDYKIENGETLKV
                     PEKPKRVAVLTGFYVGDFIKLGIKPIAVSDITKDSSILKPYLKGVDYIGENDVERVAK
                     AKPDLIVVDAMDKNIKKYQKIAPTIPYTYNKYNHKEILKEIGKLTNNEDKAKKWIEEW
                     DDKTRKDKKEIQSKIGQATASVFEPDEKQIYIYNSTWGRGLDIVHDAFGMPMTKQYKD
                     KLQEDKKGYASISKENISKYAGDYIFLSKPSYGKFDFEKTHTWQNIEAVKKGHVISYK
                     AEDYWFTDPITLEHLRSKLKKEILNKK"
     gene            3890..5197
                     /locus_tag="NBH32_001554"
     CDS             3890..5197
                     /locus_tag="NBH32_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HCD1515150.1"
                     /translation="MNKVHKPLYFYLMLFFSTTIIGALLLYLPFTGKKPISFLDALFI
                     ASSAFTVTGLSPVDIGSQFNILGEIVILLLIQIGGLGIVTVTLLTLVFLNRKISMKNR
                     FLIMVTWNIDELGGVIKLIKHLAIYSLVTELIGMICLCLSFIPKFGIGKGLFLSLFTS
                     VSAFNNAGFALFKNNLIDYSSDPIVIITISILIIFGGIGHFVVIDFINCKKLSKLSLH
                     SKLVLTTTSILIIIGAITFFLLEQFNTMQHMGLVEKIGNSFFQSVTTRTAGFNSIDIA
                     SINKSTALMLMLLMFIGGAPLSAAGGIKITTFAVAFIFVLNYIRKENNVSVFNKEISD
                     KHIKLSIVTINISFLFISIITFILSIINPNISLIKLLFEVVSAFGTVGLSMNLTTEYH
                     GITKIIIIFVMLCGKVGLLTLLRTFIPPKNPKNYRYTKGQIYL"
     gene            complement(6040..6483)
                     /locus_tag="NBH32_001555"
     CDS             complement(6040..6483)
                     /locus_tag="NBH32_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515151.1"
                     /translation="MSIITRLFNNSDFEKLNQLCKLYDDLGYPTNENDLKKRLKKITN
                     HDDYFLLLLIKENKIIGLSGMCKMMFYEKNAEYMRILAFVIHSEFRKKGYGKRLLADS
                     EEFSKRLNCKAITLNSGNRNERLSAHKLYSDNGYVSNTSGFTKQL"
     gene            complement(6589..7026)
                     /locus_tag="NBH32_001556"
     CDS             complement(6589..7026)
                     /locus_tag="NBH32_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515152.1"
                     /translation="MFISLAIWSINSMGNKQCISLNLLLSDDTFAVISVMTTLGTAIL
                     GGIAPSIYGIFTSPIDKKSSFRAISDWEQEQRNNVKDLMMLLPSTLLSILIVIFIFQF
                     AYSKLFNTIAIIIFIIILVVMFFLILLICYKITVMICRYFSKE"
     gene            complement(7343..7933)
                     /locus_tag="NBH32_001557"
     CDS             complement(7343..7933)
                     /locus_tag="NBH32_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HCD1515153.1"
                     /translation="MSELTAKQARFVNEYIRTLNVTQSAIKAGYNANSAHVTACRLLK
                     KPHIKQYIQEQKDKIIDENVLTAKELLHVLTNATVGDETETKEVVVKRGEYKENPQSG
                     KVQLVYNEHVELIEVPIKPSDRLKAREMLGKYHKLFTDKHDINGNVPIFINIGEWDGG
                     DEGLDKAVKDVSNDNPNHTVIVDDIPLEDYESIRFL"
     gene            complement(7930..8142)
                     /locus_tag="NBH32_001558"
     CDS             complement(7930..8142)
                     /locus_tag="NBH32_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pathogenicity island family protein"
                     /protein_id="HCD1515154.1"
                     /translation="MTDDNVNDHIIKNHIEMIVDRLATDKEFYIFDSLIQGLSYQDIS
                     SALDCSEQSVILWYETILDKIVGVIK"
     gene            8203..8749
                     /locus_tag="NBH32_001559"
                     /pseudo
     CDS             8203..8749
                     /locus_tag="NBH32_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002476300.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(8844..10460)
                     /gene="groL"
                     /locus_tag="NBH32_001560"
     CDS             complement(8844..10460)
                     /gene="groL"
                     /locus_tag="NBH32_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500736.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCD1515155.1"
                     /translation="MVKQLKFSEDARQAMLRGVDQLANAVKVTIGPKGRNVVLDKEFT
                     APLITNDGVTIAKEIELEDPYENMGAKLVQEVANKTNEIAGDGTTTATVLAQAMIQEG
                     LKNVTSGANPVGLRQGIDKAVKVAVEALHENSQKVENKNEIAQVGAISAADEEIGRYI
                     SEAMEKVGNDGVITIEESNGLNTELEVVEGMQFDRGYQSPYMVTDSDKMVAELERPYI
                     LVTDKKISSFQDILPLLEQVVQSNRPILIVADEVEGDALTNIVLNRMRGTFTAVAVKA
                     PGFGDRRKAMLEDLAILTGAQVITDDLGLDLKDASIDMLGTASKVEVTKDNTTVVDGD
                     GDENSIDSRVSQLKSQIEETESDFDREKLQERLAKLAGGVAVIKVGAASETELKERKL
                     RIEDALNSTRAAVEEGIVAGGGTALVNVYQKVSEIEAEGDIETGVNIVLKALTAPVRQ
                     IAENAGLEGSVIVERLKNAEPGVGFNAATNEWVNMLEAGIVDPTKVTRSALQHAASVA
                     AMFLTTEAVVASIPEKNNDQPNMGGMPGMM"
     gene            complement(10536..10820)
                     /gene="groES"
                     /locus_tag="NBH32_001561"
     CDS             complement(10536..10820)
                     /gene="groES"
                     /locus_tag="NBH32_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCD1515156.1"
                     /translation="MLKPIGNRVIIEKKEQEQTTKSGIVLTDSAKEKSNEGVIVAVGT
                     GRLLNDGTRVTPEVKEGDRVVFQQYAGTEVKRDNETYLVLNEEDILAVIE"
     gene            10997..11740
                     /locus_tag="NBH32_001562"
     CDS             10997..11740
                     /locus_tag="NBH32_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1515157.1"
                     /translation="MTRLWASLLTVIIYLLSQFLPAVIVNTTQFAQYSDLEQLKAGIY
                     IQLVLFLIAATTIIFINFKIKDPTRLESEVKEPNKYIIPWALLGFALVMVYQFIVSIV
                     LTQIYGGQQVSPNTEKIIIIARKIPIFIFFVSIIGPLLEEYVFRKVIFGELFNAIKGS
                     RIVAFIIATTVSSLIFALAHNDFKFIPVYFGMGVIFSLAYVWTKRIAVPIIIHMLQNG
                     FVVIFQLLNPEALKKATEQANFIYHIFIP"
     gene            complement(11764..13101)
                     /locus_tag="NBH32_001563"
     CDS             complement(11764..13101)
                     /locus_tag="NBH32_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515158.1"
                     /translation="MSYHWFKKMLLSTSILILSSSSLGLATHTVEAKDNLNGEKPTTN
                     LNHNITSPSVNSEMNNNETGTPHKSNQTGNEETGSNSRDANPDSNNVKPDSNNQNPSP
                     DSKPDSNNQNPSPNPKPNPDNPKPKPDPKPDPDKPKPNPDPKPNPDNPKPKPDPKPDP
                     DKPKPNPDPKPDPDNPKPNPDPKPDPDKPKPNPDPKPDPDKPKPNPNPKPDPNKPNPN
                     PSPDPDKPGDSNHSGGSKNGGTWNPNASDGSNQGQWQPNGNQGNSQNTTGNDFVSQRF
                     LALANGAYKYNPYILNQINKLGKDYGEVTDEDIYNIIRKQNFSGNAYLNGLQQQSNYF
                     RFQYFNPLKSERYYRNLDEQVLALITGEIGSMPDLKKPEDKPDSKHRSFEPHEKDDFT
                     VVKKQEDNKKSASTAYSKSWLAIVCSMMVVFSIMLFLFVKRNKKKNKNESQRR"
     gene            13298..13924
                     /locus_tag="NBH32_001564"
     CDS             13298..13924
                     /locus_tag="NBH32_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HCD1515159.1"
                     /translation="MGLFKKDKKAMTFDNAMEERRSIYNLKDSISISDDELESMIAHA
                     VKHVPSSFNSQSTRIVLLLNDNNNKFWDNTKAILKEVMGENRDFEPTEQKIDNFKHSY
                     GTILFYEDQDVVSGLQEQMPNYYDNFAIWSTQTNAMHQFAIWTALATKGIGASLQHYN
                     PLVDEMTSNEFNIPKSWKLIAQMPFGDIREAAGEKTFNPVEDRFVIKK"
     gene            14194..14979
                     /locus_tag="NBH32_001565"
     CDS             14194..14979
                     /locus_tag="NBH32_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen family hydrolase"
                     /protein_id="HCD1515160.1"
                     /translation="MKVQIYQLPIIFGDSSKNEIQITQWFEKNMNAEVDVVVLPEMWN
                     NGYDLEHLNEKADNNLGQSFSFIKHLAEKYKVDIVAGSVSNIRNNQIFNTAFSVNKSG
                     QLINEYDKVHLVPMLREHEFLTAGENVAEPFQLSNGTYVTQLICYDLRFPELLRYPAR
                     SGAKIAFYVAQWPMSRLQHWHSLLKARAIENNMFVIGTNSTGFDGNTEYAGHSIVINP
                     NGDLVGELNESADILTVDLNLNEVEQQRENIPVFKSIKLDLYK"
     gene            complement(15578..15712)
                     /locus_tag="NBH32_001566"
     CDS             complement(15578..15712)
                     /locus_tag="NBH32_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="delta-lysin family phenol-soluble modulin"
                     /protein_id="HCD1515161.1"
                     /translation="MSCLILRIFILIKEGVISMAQDIISTIGDLVKWIIDTVNKFTKK
                     "
     gene            15948..16517
                     /gene="agrB"
                     /locus_tag="NBH32_001567"
     CDS             15948..16517
                     /gene="agrB"
                     /locus_tag="NBH32_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator AgrB"
                     /protein_id="HCD1515162.1"
                     /translation="MNYFDNKIDQFATYLQKRNNLDHIQFLQVRLGMQVLAKNIGKLI
                     VMYTLAYILNIFLFTLITNLTFYLIRRHAHGAHAPSSFWCYVESILLFILLPLVIVNF
                     HINPLIMIILTVISLGIISVYAPAATKKKPIPVRLIKRKKYYAIIVSLTFFIITLIIK
                     EPFAQFIQLGIIIEAITLLPIFFIKEDLK"
     gene            16514..16654
                     /locus_tag="NBH32_001568"
     CDS             16514..16654
                     /locus_tag="NBH32_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic lactone autoinducer peptide"
                     /protein_id="HCD1515163.1"
                     /translation="MNTLFNLFFDFITGILKNIGNIAAYSTCDFIMDEVEVPKELTQL
                     HE"
BASE COUNT         5482 a         2758 c         2328 g         6096 t
ORIGIN      
        1 aatactttat cctctttaaa aataatgtgt gtacaaagtc attaatttag caaatatttt
       61 tatttagtag ttaataacca tcgatttgaa atttatatat aattattagc taaataatat
      121 cctgcatctt tctcatacaa tttactataa aattaagcat atccgatatc agcgttaata
      181 agatcgttga tactagacag ttaatttcat agaacgaaat caaataacac actactttct
      241 gcattttaaa ttatgtttaa gaataaaaat tatgtttata taaatatata tactactttg
      301 aaaggtgtga gcttaatgac aacttttagt gaaaaagaaa aaattcaatt actagcagat
      361 attgttgaac tacaaactga aaataataat gaaatagacg tttgtaatta tttaacagat
      421 ttattcgaca agtatgatat taaatctgaa attttgaaag ttaatgaaca ccgcgccaat
      481 atcgttgcag aaatcggtaa cggctcacct atactcgcat tgagtggtca tatggatgtt
      541 gttgatgcag gaaatcaaga taattggact tatccccctt ttcaactcac agaaaaagct
      601 ggcaaattat acggtcgagg cacaacggat atgaaaggcg gtctgatggc tttggtcatc
      661 acactcattg aattaaaaga acaaaatcaa ttgcctcaag gaacgataag attactagct
      721 actgctggcg aagagaaaga acaagaaggt gccaaattat tggctgataa aggctattta
      781 gacgatgtag atggcttaat tattgctgaa ccaactggat ctggaattta ttatgcacat
      841 aaagggtcta tgtcatgtaa agtaactgca actggcaaag ctgtccatag ctcagttcca
      901 tttattggtg acaatgcaat tgatacactg cttgaatttt ataatcaatt taaagaaaaa
      961 tattcagagc ttaaaaaaca tgatactaaa catgaattag atgttgcacc catgttcaaa
     1021 tcattgatag gaaaagaaat ttctgaagag gatgcaaatt atgcatctgg tcttacagct
     1081 gtatgttcga ttataaatgg cggcaaacaa tttaactctg taccagatga agcttcactt
     1141 gaatttaacg taagaccagt tcctgagtat gataacgact ttatagaatc gtttttccaa
     1201 aatatcatta atgatgtgga tagcaataag ctttcactcg atattccaag caatcaccga
     1261 cctgtaacaa gcgataaaaa tagcaaatta attactacga ttaaagatgt agcttctagt
     1321 tatgtagaac aagacgatat atttgtttcg gcgcttgtag gtgcaacaga tgcctctagt
     1381 ttcttaggtg ataataagga caatgttgat ttagccattt ttggaccagg taatccatta
     1441 atggcacatc aaatcgatga atatattgaa aaagatatgt atctgaaata tattgatatt
     1501 tttaaagaag cttccattca atatttaaaa gagaaataag aacgatgctg tcagctgccc
     1561 tattcgcgtg ctggcagttt tttattgcga attttaagtt gatagaaata acgatgaata
     1621 ttgtttgaat gtaaatattc aaatatcaac aagcacattt tcattgatta agtgatgtaa
     1681 aactgaaatt attgtgctga tttgtcatac atatattgac taatggtcat ataaaaagat
     1741 agcttctaat agttacataa aactcgtaaa aaccttctcg taaaaggaat gggacataaa
     1801 tccctagaaa acagcagctc aacgagctgt aaattatcaa atacatttga aaaaagctga
     1861 tttctatcaa ataatttata gaaatcagct tttttacatt gtctaagaac ttaatgtctc
     1921 tgtctctttg gaactttata tttcataagt actattttat tgtttaacct tttttttaaa
     1981 taaggtataa atctacaata tccaaataag ccccatcgat tcttataata gtcaaaataa
     2041 ttatattcat gtattactct tattgtattt ggtaaaattt cattcaattt ttttaaatca
     2101 ttgtaaccat atttaaaaac aggttgtgag gataactttg ataccgattg atgtctcttt
     2161 gtattattcg caattgtttt tgagcaaaat tcaatcgcaa atgtcaaatt atgatctccc
     2221 atctttttga taatagtgtc caataattga gtcattacac tctcatcaaa atacatcaat
     2281 acaccttcaa taataaacaa tatatctgac ttactattaa aaaatttata atttttgaca
     2341 tcatcaatcc aactgtaatc tagcatagat ttagatatca tcttataact attactttct
     2401 ttaaaaaatg tttttcgtat ttctattgat tctggtacat ctaaatctat ccatgatatt
     2461 ttttctttat taaatctttg aaaccttgta tctaagccac aacctatatt gaccacgatt
     2521 aaatctttat tatccttgat aagcctctta gtaacacaat ctattatcac agtacgtata
     2581 ctaattccaa tttgagacat gtcatcacat gtaacatttt tgtacatatc atctaaacca
     2641 tcaaatattt ttttagatag tgcgtctttt attattggtt tttcgttttc gtactctttt
     2701 gctcgagcta tcaaaggaat taacattgat tcgggaattc caaccagttg cttcatttaa
     2761 tcactcctta aaaacattag taccaatgca taagaaaatt tttacaactt atttctatta
     2821 ttttttattt aaaatttctt tttttaattt acttctcaaa tgttctaatg taataggatc
     2881 tgtgaaccaa taatcttctg ctttatatga aattacatgt ccttttttta cagcttcaat
     2941 attctgccat gtatgtgttt tttcaaaatc aaattttccg tacgaaggtt tacttaaaaa
     3001 tatataatca ccagcatatt tactaatatt ttcttttgaa atggaagcat agcctttttt
     3061 atcttcttgt aatttatctt tatattgctt tgtcattggc ataccgaatg catcatgaac
     3121 aatatccaaa ccacgacccc atgtagagtt atatatgtat atttgctttt catctggttc
     3181 aaacacagat gctgttgctt gaccaatttt actttgaatt tcttttttat cttttctagt
     3241 tttatcgtcc cactcttcaa tccatttttt agctttatct tcattattag tcaacttgcc
     3301 tatttctttt aatatttctt tatgattgta tttattgtat gtatatggga ttgttggcgc
     3361 tattttttgg tattttttta tatttttatc catagcatct acaacaatta aatctggttt
     3421 tgctttagca actctttcaa catcattttc tccaatataa tcaacccctt ttaaataggg
     3481 ttttaaaatt gaagaatctt tagttatatc tgaaacagca attggtttga ttcctaactt
     3541 tataaaatca ccaacataaa atccagttaa tacagcaact cttttaggtt tctctggtac
     3601 tttcagtgtt tcaccatttt caattttata gtctgtagtc tttgtcgagt tacttgtttt
     3661 atcgacagat gctgaacacg cagtcaaaat aaataaatta cataataaaa ttccaattaa
     3721 tcttttcata atatcatcct tttgtttttt aattgatatt cattttcaat tataaaatat
     3781 taaaagaatt agtcaacgcc tgttagtaat acacatcagt aacatttcta ttttcattta
     3841 tgatattatc taattattaa tttataatat aaaggagtat gaaattaaaa tgaacaaagt
     3901 ccacaaacct ttatattttt atttaatgct tttcttctct acaactatca taggtgcact
     3961 tttattgtat ttacctttca ctggtaaaaa gccaatatct tttttagatg ccctatttat
     4021 agcttcaagt gcatttacag ttactggcct atccccagtt gatataggat cacagtttaa
     4081 tatacttggt gaaatagtaa tactattatt aattcaaata ggtggtctgg gtatcgtgac
     4141 cgtaacccta ttgacactag tatttttaaa tagaaagata tcaatgaaaa atagattctt
     4201 gattatggtt acatggaata ttgacgaact tggtggtgtt attaaactaa ttaaacactt
     4261 ggctatttat agtttagtca ctgaattaat tggtatgatt tgtttgtgtt tatcttttat
     4321 accaaaattt ggtataggca aaggtttatt tttaagctta ttcacatcag tatcagcttt
     4381 taataatgct ggatttgccc tttttaagaa taacttaata gattattcta gtgatccaat
     4441 tgtcattatt acaatctcaa tactcataat atttggaggt attggacatt ttgtcgtgat
     4501 agactttatt aattgtaaaa aattgagtaa attatcttta cattctaaat tagtcttaac
     4561 tacaactagt atcctaataa ttataggagc tattacattc tttttattag aacagtttaa
     4621 tactatgcaa catatgggac tagttgaaaa aatcggaaat tcttttttcc aatcagtaac
     4681 aacacgaaca gcgggtttta acagtataga tatagcaagc attaacaaat ctaccgcctt
     4741 aatgttaatg ctacttatgt ttattggtgg tgcccctctc agtgcagctg gaggaattaa
     4801 aataactact tttgcagtag cgtttatttt tgtactaaat tatatacgta aagaaaataa
     4861 tgtttcagta ttcaataaag aaatatctga caaacatata aaactatcta ttgttaccat
     4921 taatatctca tttctattta tcagtatcat tacttttata ttatcgataa ttaatccgaa
     4981 catatcatta atcaagttat tattcgaagt ggtttctgca ttcggaacag tagggttaag
     5041 tatgaacctt accacagaat atcatggtat tactaaaata attattatat tcgttatgct
     5101 ttgcggtaaa gtaggactat taactttatt aagaacattt ataccaccaa aaaatcctaa
     5161 aaattaccgc tacactaaag gacaaattta tctataagat aactatcaat atttaacaag
     5221 aaatgaacta gtgttttaat ctattacatt taaaaaacta cctaaacagg tagtttttct
     5281 atttatttta ggcccattaa atgtcttatg tctttaatta agtaaaatgc acaagtacac
     5341 ctttaaaaag atttactcaa aactaaacta tacctagtta accgcttgtg attaagaaat
     5401 tttaaatgtg gtttctttta attatgtcaa ccacccataa ggattttaat caagcactca
     5461 ttcattcctg cactcgcaat atgcattact tatagaagtg gatgcactct catattatgg
     5521 caataccata actcccgcac cataatgatt taaataccat tatgaatctc aattacaaat
     5581 tagcacataa aaaagacgcc tcccataagt ctagtcacca aatggaaaac gtctcgtaat
     5641 taaaatatat ttgcgtgcat tctaatttgt acttagaata catcatactc gttcataatg
     5701 taattttcta attagtcaaa actatataca gttttacatc attcccggca ttatgcacta
     5761 ataaaaactt actttaatac acgctttaaa gattattaaa tcagcgtttt aatttttaga
     5821 caacgctatt atttttagtt tttcaattct attatgtcac tcaaaaactg ttttccaaca
     5881 ttgatttatt gttggaaaat ctcgaaaatc tagtccattt ctccaacaat cgaagaatta
     5941 tgcccttttt tctcccttta aataagtcat aatacgaggc atacatgcaa catttacaat
     6001 aaaatatgtt cctacatcgt attatacgaa tgctcacact tatagttgtt tagtaaaccc
     6061 agatgtattg ctaacatacc cattatcact atatagttta tgtgcagata gtctttcatt
     6121 tctattacca ctatttagtg ttattgcttt acaattcaac cgtttagaaa attcttcaga
     6181 atcagctaat aatctctttc cataaccttt tttcctaaat tcagaatgta taacaaacgc
     6241 aaggattctc atatactctg catttttttc gtaaaacatc attttacaca taccacttaa
     6301 accaattatt ttattttctt ttatcaaaag cagtaggaag taatcatcat gattcgttat
     6361 tttctttagt ctctttttta aatcattctc atttgttgga taacctagat catcatataa
     6421 tttacatagt tgatttaatt tttcaaaatc actgttatta aacaatcttg taattatgct
     6481 catataaccc accctaaaaa tattatgtac ctattgttac atacaataga tacgcaaaat
     6541 catttctcac tgcaaatcag gacgtttctc agcgtaaaca aatcattgct attcttttga
     6601 aaaatatctg catatcatca cagtaatttt ataacagatt aataaaatta aaaaaaacat
     6661 aacaacaagt attataataa atattataat agctatcgta ttaaatagtt tagaatatgc
     6721 aaactgaaaa ataaaaatca caattaaaat actaagtaat gtagatggta atagcatcat
     6781 cagatcttta acattatttc tttgttcttg ctcccaatcg ctaatagccc taaaactact
     6841 ttttttatct ataggcgatg taaaaatacc atatattgaa ggtgctatac ctcctaaaat
     6901 agcagttccc aaagttgtca ttactgaaat tactgcgaaa gtatcatccg aaagcaataa
     6961 attcaaacta atgcattgtt tattacccat cgaatttatt gaccaaatag ctagagaaat
     7021 aaacaaccca aaatttaaaa taaatgatat agtaatagca attgttgaca aaacacgaaa
     7081 tttttcattt ttatttatat tatccatttt ctcccttttt cttaaatcat tttattatat
     7141 attacaataa ttaatctgaa atgttgatgt aatttgaaaa aaatatcata cttttactcc
     7201 tgaaaacctc cctaaatcat caatatgaaa atcagtatta ggatattgcg tttacaactc
     7261 ttttaaatct cactcattct tctcatcgtc ttaaccgata ctatcaataa atactggcaa
     7321 caccataaaa tatataccca atttataaaa acctaattga ctcataatct tctaacggta
     7381 tatcatccac aatcacagta tgattaggat tatcgttaga tacatctttc actgccttat
     7441 ctaacccctc atcacctccg tcccattcac caatattaat gaatataggt acattaccgt
     7501 ttatatcatg cttatctgta aataatttat gatatttacc caacatttca cgagctttta
     7561 aacgatcact aggttttatt ggtacctcta tcagttcaac atgttcatta tagactaatt
     7621 gtactttgcc actttgtgga ttttctttat attctccacg cttgactaca acttctttcg
     7681 tttctgtttc atcaccgact gtcgcattcg ttagcacatg taataactct tttgcggtta
     7741 atacattctc atctataatc ttatcttttt gttcttgtat atattgcttg atgtgcggct
     7801 ttttcaataa cctacacgct gtcacatgtg cgctatttgc gttatatcct gcttttatgg
     7861 cactttgtgt tacattcagt gttctaatat actcattcac aaaacgtgct tgctttgcag
     7921 ttaactcact cattttatca cccccacaat tttatctaat atggtttcat accataatat
     7981 tacagattgt tctgaacaat ctaaggcact actaatatct tgataactaa gtccttgtat
     8041 aagggagtca aaaatataaa actctttatc ggtcgctaat ctgtcaacaa tcatttctat
     8101 gtgattcttt ataatatgat cattgacatt atcgtctgtc atcaattcgt cagaatcttc
     8161 atcacctatt aaaaagaaat catcagtatt tatttcatca tcgccccgtt aactagcttt
     8221 gaagtcttta gcacacttgc atataccggc tgtcgtgctg gcgagatact agcattgaag
     8281 tggtctgata ttgattttga aaacaacacg attagtatta ctaaaacata ttacaatcca
     8341 aataataaca agaagaaatt tcagatactt ccccctaaaa ctgaaagttc tatcggtaag
     8401 atttccgttg acccaaatgt aataaaggtg ttgcgtgatt ataagataaa cgttcaaaat
     8461 aattggaaaa acgaattata taatgataac cattgtaatg aaaaaactat caatgtggat
     8521 acaaaccatt atgtcacgta ccagcatact caaaaatatc tctacgcact cattcagata
     8581 cacacattgc gcattgctca tagaatcagg tgtacacatt aaagaaatac aagaacgatt
     8641 acggcataaa gatatcaata ccactatgaa tatctatgct aaaatcacaa attcatacaa
     8701 aaaagacgcc tcccataagt ttagtcacca aatggaagac gtctcgtaat taaaatatat
     8761 ttgcgtgcat tctaatttat acttagaatg aatcatactc gtgcataatg taattttcta
     8821 gttagtcaaa actataaaca gttttacatc attcctggca tgccacccat gttaggttgg
     8881 tcattatttt tttctggaat tgatgctaca accgcttcag tcgttaagaa cattgctgca
     8941 acacttgctg catgttgtaa tgctgagcgt gttactttag ttggatcaac gatacctgct
     9001 tctaacatat taacccactc gtttgtagca gcgttaaaac caacacccgg ctctgcgttt
     9061 ttcaaacgtt ctacaataac agaaccttct aatcctgcat tttcagcaat ttgacgaact
     9121 ggtgcagtta gtgctttaag tacaatattt acacctgttt caatgtcacc ttctgcttca
     9181 atttcactta ctttttggta aacatttact aatgcagtac caccacctgc aacaatacct
     9241 tcttcaactg ctgcacgtgt agaatttaat gcatcttcaa tacgtaattt acgttcttta
     9301 agctctgttt cacttgctgc acctacttta ataactgcaa caccacctgc taatttagct
     9361 aagcgctctt gtaatttttc acgatcaaag tcagattcag tttcttcaat ttgagatttc
     9421 aattggctta cgcgtgaatc aatgctgttt tcgtcaccgt caccatcaac aacagtggta
     9481 ttatctttag ttacttctac tttacttgca gtacctaaca tatcaattga tgcatctttt
     9541 aaatctaagc ctaaatcatc agtaatcact tgcgcaccag ttaaaatagc taaatcttct
     9601 aacattgctt tacgacgatc accgaaacca ggcgccttta ctgcaacagc tgtaaatgtg
     9661 ccacgcatac ggtttagcac gatatttgtt aatgcatcgc cttcaacttc atcagctaca
     9721 attaagattg gacgattaga ttgaaccact tgttctaata aaggtaagat atcttggaaa
     9781 gacgagattt tcttatctgt cactaaaata tatgggcgtt ctaattcagc gaccatttta
     9841 tctgaatcag taaccatata cggtgattga taaccacgat caaattgcat accttcaacc
     9901 acttcaagtt ctgtattcaa gccatttgac tcttcaattg taatgacacc atcattacct
     9961 actttttcca tagcttcaga aatataacgt ccaatttctt catctgctgc agaaattgca
    10021 cctacttgcg caatttcatt tttattttca actttttgag aattttcatg taacgcttca
    10081 acagcaactt taactgcttt gtcgatacct tgtcgtaaac caactgggtt cgcaccactt
    10141 gtaacatttt tcaagccttc ttgaatcatt gcttgagcta atactgttgc agttgtcgta
    10201 ccgtcaccag caatttcatt tgtcttattt gcgacttctt gaactagttt agcccccata
    10261 ttttcatatg gatcttctaa ttcgatttct ttagcaatcg ttacaccatc gttcgtaatt
    10321 aaaggtgctg taaactcttt atctaataca acattacgtc ctttaggacc aatcgttact
    10381 ttaactgcat ttgcaagttg gtcaacacca cgtaacattg cttgacgtgc atcttcagag
    10441 aatttcaatt gtttaaccat aaatgataaa cctccatttg tttagttgtg tttcattttc
    10501 gttcttttta atttatttat gaattaagtt ctgtattatt caataactgc taaaatatct
    10561 tcttcattta ataccagata tgtttcatta tctcgtttaa cttctgtacc agcatattgt
    10621 tggaacacga cacggtcccc ttctttcact tcaggagtca ctcttgtacc atcatttaat
    10681 agacgtccag ttcctactgc aacgataacg ccttcgtttg atttttcttt agcactatca
    10741 gttaaaacaa taccactttt agttgtttgt tcttgttctt ttttctcaat aatcacacga
    10801 tttccaattg gttttagcat gattgttcct ccttaaaaaa acctaaagtt tagcacttaa
    10861 aattaaagag tgctaacata catttataat aatcaaattt ggtcaaaatt tcaagtcata
    10921 accttttaat taagttttgt aacttcaagt tatttaagat aaaataagtt ataaataaaa
    10981 tatgtggagg aaaattatga caagactatg ggcatcattg ctaactgtta ttatatattt
    11041 attgtcgcaa tttttaccgg cagtcattgt aaacacaaca caatttgcac aatatagtga
    11101 tttggaacaa cttaaagccg gcatctatat tcaactcgtt ctatttttaa ttgcggctac
    11161 tacgattatt tttattaatt ttaaaatcaa agaccctaca agattagaat ctgaagtaaa
    11221 ggaacctaat aaatacatta ttccatgggc attacttgga tttgcacttg tcatggttta
    11281 tcaatttata gtgagcattg tattaacgca aatttatggt ggacaacaag taagtcctaa
    11341 tacagaaaag ataattatta ttgctcgaaa aatacctata tttatcttct ttgtatctat
    11401 tattggtcct ttattagaag aatatgtatt cagaaaagta atctttggag aattatttaa
    11461 tgcgattaaa ggtagtcgta tcgtggcatt tattattgct acaacagtaa gttcattaat
    11521 atttgcatta gcacataatg atttcaaatt tattccagtt tattttggta tgggtgtcat
    11581 tttttcatta gcatatgttt ggacaaaacg gattgctgtt ccaattatta tccatatgtt
    11641 acaaaacgga tttgtcgtta tattccaatt acttaatcct gaggctttga aaaaagccac
    11701 ggaacaagcg aattttatat atcacatttt tattccataa taaaaaaacg aatcacacac
    11761 ggattatcgt cgctgtgatt cgtttttatt tttcttttta tttcgcttta caaataagaa
    11821 tagcatgatt gaaaatacca ccatcataga acatacaatt gctagccaac ttttactata
    11881 tgcagttgac gcacttttct tattatcttc ttgtttttta actactgtaa aatcgtcttt
    11941 ttcatgcggt tcaaatgaac ggtgttttga atccggctta tcttcgggct ttttcaaatc
    12001 tggcattgat ccaatttcac cagtaattaa tgcgagtacc tgttcatcta aattacgata
    12061 atatcgttct gattttaaag gattaaaata ttggaaccta aaatagtttg attgttgttg
    12121 taatccattt aaatatgcat ttccgctgaa gttttgtttt cgaataatat tataaatgtc
    12181 ttcatcagta acttctccat aatctttgcc caacttatta atttgattta aaatatacgg
    12241 attatacttg taagccccat tcgccaaggc taaaaatcgt tgggatacaa aatcattacc
    12301 agtagtattt tgtgagtttc cttgatttcc atttggttgc cattgacctt gattagatcc
    12361 atctgaagca tttgggttcc atgttccccc atttttcgag ccaccagaat gattggaatc
    12421 cccaggttta tctggatctg gtgacgggtt aggatttggc ttattagggt ctggttttgg
    12481 attcggattt ggctttggtt tatctggatc tggttttgga tctggatttg gctttggttt
    12541 atctgggtct ggttttggat ctggatttgg tttcgggtta tctggatctg gttttggatc
    12601 cggatttggc tttggtttat ctgggtctgg ttttggatcc ggttttggtt tcgggttatc
    12661 tggatttggt tttggatccg gatttggctt tggtttatct gggtctggtt ttggatccgg
    12721 ttttggtttc gggttatctg gatttggttt tggattcgga cttgggtttt ggttatttga
    12781 gtctggtttt gaatctggac ttgggttttg gttgtttgag tctggcttca cattattcga
    12841 atcaggatta gcatcacgac tattcgaacc tgtttcttca ttacccgttt gatttgattt
    12901 gtgaggtgtc ccagtctcat tattattcat ttcactattt actgatggtg aagttatatt
    12961 atgattcaaa ttagtagttg gcttttctcc atttaagtta tcctttgctt caactgtgtg
    13021 cgttgcaagc cctaaactac tactacttaa aattaaaata cttgttgaaa gtaacatttt
    13081 cttaaaccaa tgatatgaca tttcacgaga cacctccctt tttcatgtaa gtattaatct
    13141 aagtataatc aatatgcgtt gaaaatgcat ttatatttgg aatttgaaaa tgaatattaa
    13201 tattcttgaa attttcgtaa atattacttt gcaagttcga gtataaattt tatacttagt
    13261 ctattaagta ttctttacta aagaaggagc aataattatg ggtttattta aaaaagataa
    13321 aaaagcaatg acttttgaca atgcaatgga agaaagacgt tcaatatata atttgaaaga
    13381 ttcgatatca attagcgatg acgaattaga atcaatgatt gcacatgcag ttaaacatgt
    13441 tccttcatca tttaattctc aatcaactag aattgttctc ttgttaaatg acaacaataa
    13501 taaattttgg gataacacaa aagcaatttt aaaagaagtc atgggagaga atcgtgattt
    13561 cgagccaact gaacaaaaaa ttgataactt caaacattca tacggcacaa ttttgtttta
    13621 tgaagatcaa gatgttgtaa gtggtttaca agaacaaatg ccaaactact atgataattt
    13681 tgcaatatgg tcaactcaaa caaatgccat gcatcaattt gcaatttgga ctgcattagc
    13741 tactaaaggc ataggtgctt cattacaaca ctataatcca cttgttgatg agatgacttc
    13801 aaatgaattt aatattccta aaagttggaa acttattgct caaatgccat ttggtgatat
    13861 tcgtgaagct gctggtgaaa aaacatttaa tccagtggaa gatcgtttcg taataaaaaa
    13921 ataatcaata ttttgttagg aatggtacag aaatgataaa gaatcattag ttatccatta
    13981 tgtagtagtt cttacacatt agccgctgct aatgtgcact taagaatagg aatacatgag
    14041 taaaactcat gtataagaaa tactaatttc taaagaaaaa gtatttcttt atgttagagc
    14101 ctaggacttt gattttatgt cctgggctcg ttttttgcgt ttcaacataa cttttagtaa
    14161 gttatatata agatctatca aggatgtgat gttatgaaag tccaaattta tcaattaccg
    14221 attatttttg gagatagttc taaaaatgaa attcaaataa cgcaatggtt cgaaaaaaat
    14281 atgaatgctg aagtagatgt agtcgtttta cctgaaatgt ggaataatgg ctatgattta
    14341 gagcatttaa atgaaaaagc agataataat ctaggacaaa gtttttcatt tattaaacat
    14401 ttagctgaaa aatataaagt cgatatcgta gctgggtcag tttctaacat tagaaacaat
    14461 caaatattta atacagcttt tagtgtgaat aaaagtggcc agctgattaa tgaatacgac
    14521 aaagttcatt tagttcctat gttaagagag catgaatttt taaccgctgg cgaaaacgta
    14581 gctgaacctt ttcaattgtc taacggtact tatgtgaccc aacttatctg ttatgattta
    14641 cgttttccag aattattaag atatcctgct cgtagtggtg ctaaaattgc attttatgtg
    14701 gcgcaatggc ctatgtcacg tcttcaacat tggcattcat tactaaaggc acgtgcaatt
    14761 gaaaataata tgtttgtcat tggaactaat agcactggat tcgatggtaa cacagaatat
    14821 gctggtcatt cgatagttat aaatccaaac ggagatttag ttggagaatt aaatgaatcc
    14881 gcagatattt tgactgtaga tttaaactta aatgaagtag aacagcaacg cgaaaatata
    14941 cctgtattta aaagtattaa attagatttg tataaataag aagcgcctga aataatattt
    15001 aacacatgtc atgactaaac atagatttat gagagatttt tcataagtct atgtttattt
    15061 tttataatag ataactcagt aagaatccat ttcgcccttt gcaaatgaat gattttctta
    15121 attaaattga ttaatttttt gaccttttcc aacattagac ttatttatat attttaacgg
    15181 tggctctcat aatgaggatt aactctcccc ttctttatta caataaaaaa ggccgcgagc
    15241 ttgggagggg ctcacgacca tacttattat taagggaatg ttttacagtt attttttcaa
    15301 tctatttttg gggatgttat taattatcaa aaaaatttta tttatcaaat aatgataaat
    15361 cgatgttgtt tacgatagct tacatgctag aaataattat ctgtaatgat aattaagaaa
    15421 aatacatagc actgagtcca aggaaactaa ctctactagc aaatgttact cacttgctaa
    15481 ttttacatgg ttattaagtt gggatggctt aataactcat acttttagta ctatacgaag
    15541 ataacaaatt tacaatgaaa gtaattaatt attcatctta ttttttagtg aatttgttca
    15601 ctgtgtcgat aatccatttt actaagtcac cgattgttga aatgatatct tgtgccattg
    15661 aaatcactcc ttccttaatt aagataaaaa ttcttaaaat taaacaactc atcaactatt
    15721 ttccatcaca tctctgtgat ctagttatat taaaacatgc taaaagcatt tattttccaa
    15781 tttttcttaa ctagtcgttt tttattctta actgtaaatt tttttatgtt aaaatattaa
    15841 atacaaatta catttaacag ttaagtattt atttactaca gttaggcaat ataatgataa
    15901 aagattgtac taaatcgtat aatgacagtg aggagagtgg tgtaaaattg aattattttg
    15961 ataacaaaat tgaccagttt gccacgtatc ttcaaaagag aaataactta gatcatattc
    16021 aatttttgca agtacgatta gggatgcagg tcttagctaa aaatataggt aaattaattg
    16081 ttatgtatac tcttgcctat attttaaaca tttttctgtt tacgttaatt acgaatttaa
    16141 cattttactt aataagaaga catgcacatg gtgcacatgc accttcttcc ttttggtgtt
    16201 atgtggaaag tattttatta tttatactct tacctttagt aatagtaaat tttcatatta
    16261 atccattaat tatgattatt ttaacagtta tttctttagg tataatctca gtatatgctc
    16321 ctgcagcaac taaaaagaag cccattcctg tgcgacttat taaacgaaaa aaatattatg
    16381 cgattattgt tagtttaacc tttttcatta tcacacttat catcaaagag ccatttgccc
    16441 aattcattca attaggcatc ataatagaag ctattacatt attacctatt ttctttatta
    16501 aggaggactt aaaatgaata cattatttaa cttatttttt gattttatta ctgggatttt
    16561 aaaaaacatt ggtaacatcg cagcttatag tacttgtgac ttcataatgg atgaagttga
    16621 agtacctaaa gaattaactc aattacacga ataataaaaa taga
//
LOCUS       DAHHOJ010000056        16531 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.056, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000056 DAHHOJ010000000
VERSION     DAHHOJ010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16531)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16531)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16531
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.056"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            147..908
                     /gene="sufC"
                     /locus_tag="NBH32_001569"
     CDS             147..908
                     /gene="sufC"
                     /locus_tag="NBH32_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCD1515164.1"
                     /translation="MASTLEIKDLHVSIEDKEILKGVNLTINTDEIHAIMGPNGTGKS
                     TLSSAIMGHPSYEVTKGEVLLDGVNILELEVDERAKAGLFLAMQYPSEITGVTNADFM
                     RSAINAKREEGQEINLMQFIKKLDKNMDFLDIDKDMAQRYLNEGFSGGEKKRNEILQL
                     MMLEPKFAILDEIDSGLDIDALKVVSKGINQMRGENFGALMITHYQRLLNYITPDKVH
                     VMYAGKVVKSGGPELAKRLEEEGYEWVKEEFGSAE"
     gene            1006..2313
                     /gene="sufD"
                     /locus_tag="NBH32_001570"
     CDS             1006..2313
                     /gene="sufD"
                     /locus_tag="NBH32_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCD1515165.1"
                     /translation="MTTDILNIFEEQLVDYSKAHNEPSWMTELRKKALKLTETLEMPK
                     PDKTKLRKWDFDSFKQHDVKGDVYQSLSQLPESVREIIDVDHSKNLVIQHNNTIAYTQ
                     VDDNASKDGVIVEGLADALMNHSDLVQKYFMKDAVTVDEHRITALHTALVNGGVFVYV
                     PKNVVVEHPVQYVVLHDDENASFYNHVIIVTEESAEVTYVENYLSNASGEGNQLNIIS
                     EVIAGANSNITYGSVDYMDKGFTGHIIRRGITEADASINWALGLMNEGSQIIDNTTNL
                     FGDRSTSSLKSVVVGTGEQKINLTSKIVQYGKETDGYILKHGVMKEHASSVFNGIGYI
                     KHGGTKSIANQESRVLMLSEHARGDANPILLIDEDDVQAGHAASVGRVDPDQLYYLMS
                     RGISQREAERLVIHGFLDPVVRELPIEDVKRQLREVIERKVSK"
     gene            2419..3669
                     /locus_tag="NBH32_001571"
     CDS             2419..3669
                     /locus_tag="NBH32_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCD1515166.1"
                     /translation="MNEVAEHSFDVNEVIKDFPILDQKVNGKRLAYLDSTATSQTPVQ
                     VLNVLEDYYKRYNSNVHRGVHTLGSLATDGYENARETVRRFINAKYFEEIIFTRGTTA
                     SINLVAHSYGDANVEEGDEIVVTEMEHHANIVPWQQLAKRKNATLKFIPMTADGELNI
                     EDIKQTINDKTKIVAIAHISNVLGTINDVKTIAEIAHQHGAIISVDGAQAAPHMKLDM
                     QEMNADFYSFSGHKMLGPTGIGVLFGKRELLQKMEPIEFGGDMIDFVSKYDATWADLP
                     TKFEAGTPLIAQAIGLAEAIRYLERIGFDAIHKYEQELTIYAYEQMSAIEGIEIYGPP
                     KDRRAGVITFNLQDVHPHDVATAVDTEGVAVRAGHHCAQPLMKWLNVSSTARASFYIY
                     NTKEDIDQLINALKQTKEFFSYEF"
     gene            3659..4123
                     /locus_tag="NBH32_001572"
     CDS             3659..4123
                     /locus_tag="NBH32_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HCD1515167.1"
                     /translation="MNFNNLDQLYRSVIMDHYKNPRNKGVLDNGSMTVDMNNPTCGDR
                     IRLTFDIEDGIIKDAKFEGEGCSISMASASMMTQAVKGHSLGEAMQMSQEFTKMMLGE
                     DYVITEEMGDIEALQGVSQFPARIKCATLAWKALEKGTVAKEGKAEGTTEEE"
     gene            4274..5671
                     /gene="sufB"
                     /locus_tag="NBH32_001573"
     CDS             4274..5671
                     /gene="sufB"
                     /locus_tag="NBH32_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019469760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCD1515168.1"
                     /translation="MAKKAPDVGDYKYGFHDDDVSIFRSERGLTENTVREISNMKNEP
                     EWMLDFRLKSLKLFYKMPMPQWGGDLSELNFDDITYYVKPSEQAERSWDEVPEEIKRT
                     FDKLGIPEAEQKYLAGVSAQYESEVVYHNMEKELEEKGIIFKDTDSALQENEELFKKY
                     FASVVPAADNKFAALNSAVWSGGSFIYVPKNIKLDTPLQAYFRINSENMGQFERTLII
                     ADEGASVHYVEGCTAPVYTTSSLHSAVVEIIVHKDAHVRYTTIQNWANNVYNLVTKRT
                     FVYENGNMEWVDGNLGSKLTMKYPNCVLLGEGAKGSTLSIAFAGKGQVQDAGAKMIHK
                     APNTSSTIVSKSISKNGGKVIYRGIVHFGRKAKGARSNIECDTLILDNESTSDTIPYN
                     EVFNDQISLEHEAKVSKVSEEQLFYLMSRGISEEEATEMIVMGFIEPFTKELPMEYAV
                     EMNRLIKFEMEGSIG"
     gene            5857..5996
                     /locus_tag="NBH32_001574"
                     /pseudo
     CDS             5857..5996
                     /locus_tag="NBH32_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499406.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6002..6316)
                     /locus_tag="NBH32_001575"
                     /pseudo
     CDS             complement(6002..6316)
                     /locus_tag="NBH32_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499407.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6681..7721
                     /locus_tag="NBH32_001576"
     CDS             6681..7721
                     /locus_tag="NBH32_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCD1515169.1"
                     /translation="MIIAIIILIFISFFFSGSETALTAANKTKFKTEADKGDKKAKGI
                     VKLLEKPSEFITTILIGNNVANILLPTLVTIMALRWGISVGIASAVLTVVIILISEVI
                     PKSVAATFPDKITRLVYPIINICVIVFRPITLLLNKLTDSINRSLSKGQPQEHQFSKE
                     EFKTMLAIAGHEGALNEIETSRLEGVINFENLKVKDVDTTPRINVTAFASNATYEEVY
                     ETVMNKPYTRYPVYEGDIDNIIGVFHSKYLLAWSNKKEDQITNYSAKPLFVNEHNKAE
                     WVLRKMTISRKHLAIVLDEFGGTEAIVSHEDLIEELLGMEIEDEMDKKEKEKLSQQQI
                     QFQQRKNRNVSI"
     gene            7735..8802
                     /locus_tag="NBH32_001577"
     CDS             7735..8802
                     /locus_tag="NBH32_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HCD1515170.1"
                     /translation="MWNKNRLTQMLSIEYPIIQAGMAGSTTPKLVASVSNSGGLGTIG
                     AGYFNTQQLEDEIDYVRQLTSNSFGVNVFVPSQQSYTSSQIENMNAWLKPYRRALHLE
                     ELVVKITEEQQFKCHIDTIIKKQVTVCCFTFGIPNESIIKRLKEANIKLIGTATSVDE
                     AIANEKAGMDAIVAQGSEAGGHRGSFLKPKNQLPMIGTISLVPQIVDVVSIPVIAAGG
                     IMDGRGVLASIVLGAEGVQMGTAFLTSQDSNASELLRDAIINSKETDTVVTKAFSGKL
                     ARGINNRFIEEMSQYEGDIPDYPIQNELTSSIRKAAANIGDKELTHMWSGQSPRLATT
                     HPANTIMSNIINQINQIMQYK"
     gene            9056..9139
                     /locus_tag="NBH32_001578"
     CDS             9056..9139
                     /locus_tag="NBH32_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515171.1"
                     /translation="MIIIKRKHQAEERRKILIKQKHQTKVR"
     gene            9187..10035
                     /locus_tag="NBH32_001579"
     CDS             9187..10035
                     /locus_tag="NBH32_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCD1515172.1"
                     /translation="MINIGLTGWGDHYSLYEDLERQTDKLKTYAGHFPVVELDATYYA
                     IQPERNILKWIKETPDTFEFVVKIHQALTLHADYKTFADTRQELFDQFKNMLEPLHTQ
                     KKLAMVLVQFPPWFDCNAQNIKYILYVRQQLQAFPMCVEFRHQSWFSDAFKEQTLAFL
                     TEHQIIHAVVDEPQVKDGSVPLVNRITNEIAFVRYHGRNHYGWTKKDMSDQEWRDVRY
                     LYDYNEQELIDLAQKAQILAQKAKKVYVIFNNNSGGHAANNAKTYQRLLNIEYEGLAP
                     QQLKLF"
     gene            10048..10875
                     /locus_tag="NBH32_001580"
     CDS             10048..10875
                     /locus_tag="NBH32_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCD1515173.1"
                     /translation="MLLTITLLVLIGGLSAIIGSIVGIGGGIIIVPTMVYLGVEHGLL
                     HNITTQVAIGTSSVILIVTGLSSSLGYLKTKQVDIKNGSIFLFGLLPGSLLGSFISRY
                     LTFESFNLYFGIFLIFVAILLMVRNKIKPFKIFDKPKYEKTYVDAKGKTYHYSVPPLF
                     AFVTTFLIGILTGLFGIGGGALMTPLMLIVFRFPPHVAVGTSMMMIFFSSVMSSIGHI
                     AQGHVAWGYAIILIISSYFGAKIGVKVNQSIKSDTVVTLLRTVMLLMGIYLIIRALI"
     gene            10902..12221
                     /locus_tag="NBH32_001581"
     CDS             10902..12221
                     /locus_tag="NBH32_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1515174.1"
                     /translation="MRLTIYHTNDIHSHLHEYERIKAYMAEHRPRLNHPSLYVDLGDH
                     VDLSAPITEATLGKKNVALLNEAKCDVATIGNNEGMTISHEALNHLYDEAKFIVTCSN
                     VIDESGHLPNNIVSSYIKDIDGVKILFVAATAPFTPFYRALDWIVTDPLESIKEEIEL
                     QRGKFDVLIVLSHCGIFFDETLCQELPEIDVIFGSHTHHYFEHGEINNGVLMAAAGKY
                     GNYLGEVNLTFEAHKVVHKTAKIIPLETLPEVETSFEEEGKTLMSNSVIQHPVVLKRS
                     MNHITEAAYLLAQSVCEYTHAQCAIINAGLLVKDIVKDEVTEYDIHQMLPHPINMVRV
                     RLSGVKLKEIIAKSNKQEYMYEHAQGLGFRGNIFGGYILYNLGYIHSTGRYYLNGEEI
                     EDDKEYVLGTIDMYTFGRYFPILKELPKEYLMPEFLRDIFKEKLLEY"
     gene            12305..13222
                     /gene="lipA"
                     /locus_tag="NBH32_001582"
     CDS             12305..13222
                     /gene="lipA"
                     /locus_tag="NBH32_001582"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCD1515175.1"
                     /translation="MATKNEEILRKPDWLKIKLNTNENYTGLKKMMREKNLNTVCEEA
                     KCPNIHECWGARRTATFMILGAVCTRACRFCAVKTGLPNELDLNEPERVAESVELMNL
                     KHVVITAVARDDLRDAGSNVYAETVRKVRERNPFTTIEILPSDMGGDYDALETLMASR
                     PDILNHNIETVRRLTPRVRARATYDRTLEFLRRSKELQPDIPTKSSIMVGLGETIEEI
                     YETMDDLRANDVDILTIGQYLQPSRKHLKVQKYYTPLEFGKLRKVAMDKGFKHCQAGP
                     LVRSSYHADEQVNEAAKEKQRQGEAQLNS"
     gene            13352..13735
                     /locus_tag="NBH32_001583"
     CDS             13352..13735
                     /locus_tag="NBH32_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HCD1515176.1"
                     /translation="MIKVDQHYFELIENYRECFNEEQFIARYSDILDKYDYIVGDYGY
                     DQLRLKGFYKDSNKKAEMSKRFSNIQDYIFEYCNFGCPYFVLRHLSKQEVKKLIEEVH
                     PSDVIDDDNKLQDVKIKPTIQETEH"
     gene            complement(13814..14080)
                     /locus_tag="NBH32_001584"
     CDS             complement(13814..14080)
                     /locus_tag="NBH32_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3055 domain-containing protein"
                     /protein_id="HCD1515177.1"
                     /translation="MIDMYLYDDNEESQVQFVGFVGEHSRYDLMLVHTNRHYGKTLVL
                     NMQTNKFGIIGTDDLKEEGYIAHILGVNAEEGDEITEYLNEVIH"
     gene            14178..14612
                     /locus_tag="NBH32_001585"
     CDS             14178..14612
                     /locus_tag="NBH32_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF86 domain-containing protein"
                     /protein_id="HCD1515178.1"
                     /translation="MYFVDKDKLTQKLAYLQALTDDYHESKHNHYAFERIAQMLIESS
                     VDIGNMIIDAFILRDPGNYKDVIDILELENVITKETQQAINKTVDIRKQFTYDYTALD
                     IKVIMPMFDDALPYYKQFITEVTTFLHQENVPVTAFGKGENQ"
     gene            14612..15397
                     /locus_tag="NBH32_001586"
     CDS             14612..15397
                     /locus_tag="NBH32_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000816183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HCD1515179.1"
                     /translation="MKQYKAYLIDLDGTMYMGTDEIDGAKQFIDYLNVKGIPHLYVTN
                     NSTKTPEQVTEKLREMHIDAKPEEVVTSALATADYISEQSPGASVYMLGGSGLNTALT
                     EAGLVIKNDEHVDYVVIGLDEQVTYEKLAIATLGVRNGATFISTNPDVSIPKERGFLP
                     GNGAITSVVSVSTGVSPQFIGKPEPIIMVKALEILGLDKSEVAMVGDLYDTDIMSGIN
                     VGMDTIHVQTGVSTLEDVQNKNVPPTYSFKDLNEAIAELEKYM"
     gene            15424..16383
                     /locus_tag="NBH32_001587"
     CDS             15424..16383
                     /locus_tag="NBH32_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HCD1515180.1"
                     /translation="MVKIVVSRKIPDKFYQQLSKLGDVVMWQKSLVPMPKDQFATALR
                     DADACFITLSEQIDAEILAQSPNLKVIANMAVGYDNIDVESATANNVVVTNTPNVLTE
                     TTAELGFTLMLAIARRIVEAEKYVEADAWQSWGPYLLSGKDVFNSTIGIYGMGDIGKA
                     FARRLQGFNTNILYHNRSRHKDAEADFNATYVSFEKLLAESDFIICTAPLTKETHHKF
                     NAEAFEQMKNDAIFINIGRGQIVDETALIDALDNKEILACGLDVLANEPIDHTHPLMG
                     RDNVLITPHIGSASVTTRDNMIQLCINNIEAVMTNQVPHTPVN"
BASE COUNT         5974 a         2363 c         3147 g         5047 t
ORIGIN      
        1 ataataaaaa gggtttaagt tgtttatatt aaggtataag taagttataa ttaactgaac
       61 gcattattac aaagtctttt tgactacaaa ttaaaattat tataaactag ttaagaaaac
      121 tttatatttt acggagggaa tataaaatgg catcaacatt agaaatcaaa gacctacatg
      181 tgtctattga ggataaagaa atcttaaaag gtgttaactt gacaattaac actgatgaaa
      241 tacatgcgat tatgggacca aacgggacag gtaaatcaac tttatcatct gcaattatgg
      301 gacacccaag ctatgaagta actaaaggag aagtactttt agacggtgta aatattttag
      361 aattagaagt tgatgaaaga gcaaaagcag gattattctt ggcaatgcaa tatccatcag
      421 aaattacagg tgttacaaat gctgatttca tgcgttcagc aatcaatgcg aaacgtgaag
      481 aaggacaaga aatcaactta atgcaattta ttaagaaatt agataaaaat atggattttc
      541 tagacataga taaagacatg gcacaacgtt atttaaatga aggtttctca ggtggagaga
      601 agaaacgtaa cgaaatctta caattaatga tgttagaacc taagtttgca atcttagatg
      661 aaatcgattc agggttagac atcgatgcat taaaagttgt atctaaaggt attaaccaaa
      721 tgcgtgggga aaactttggt gcattaatga ttacacacta tcaacgatta ttaaattaca
      781 ttactcctga taaagtacat gtaatgtatg ctggtaaagt cgttaaatct ggtggtccag
      841 aattagcaaa acgtcttgaa gaagaaggat atgaatgggt taaagaagag ttcggttcag
      901 ctgaataatc ttattaatac agtatccatg agatgttcat ctatatatga tgaaaatgaa
      961 catttatacg aaatagtaaa tttcatcaag taggaggaaa aagttatgac aactgatatt
     1021 ttgaacattt ttgaagaaca acttgttgat tattctaaag cccacaatga accttcttgg
     1081 atgacagaat tacgtaaaaa agctttgaaa ttaacagaaa ctttagaaat gccaaaacct
     1141 gataaaacaa aattaagaaa atgggatttt gattctttta aacaacacga tgtaaaaggt
     1201 gatgtttatc aatctttatc acaattacct gagtcagtaa gagaaattat tgacgtagat
     1261 cattctaaaa acttagtaat tcaacataat aatacgattg cgtacacaca agttgatgat
     1321 aatgcatcga aagatggcgt tatcgttgaa ggtttagcag acgctcttat gaaccatagt
     1381 gatttagtac aaaagtactt tatgaaagat gcagtaacag tagatgaaca tcgtatcaca
     1441 gcgctacaca cggcattagt taatggtggc gtatttgttt atgttcctaa aaatgtggtt
     1501 gtagaacatc cagtacaata cgttgtgttg cacgacgacg aaaatgcaag cttttataac
     1561 catgttatca tcgttactga agaaagcgcc gaagtcacat atgttgaaaa ttacttatca
     1621 aatgcatctg gtgaaggaaa tcaattaaat attatttctg aagtgattgc tggcgcaaac
     1681 tccaatatca catatggctc agtggactat atggataaag gctttacagg tcatatcatt
     1741 cgacgtggta ttactgaagc ggatgcctca attaattggg cattaggttt aatgaatgag
     1801 ggtagccaaa ttattgataa tacaacaaat ttatttggtg atcgttcaac aagttcactt
     1861 aaatcagtag ttgtaggtac aggcgaacaa aaaattaatc taacatctaa aatcgtacaa
     1921 tatggtaaag aaacagatgg ttatatcctt aagcatggtg ttatgaaaga acatgcatca
     1981 tctgtattta atggtatcgg ctacattaag catggtggaa ctaaatcaat tgctaatcag
     2041 gaatcacgtg tattaatgtt atctgaacat gctcgtggtg acgcgaatcc tattttatta
     2101 attgatgaag atgatgtaca agctggtcat gctgcatcag taggtcgtgt tgatccagat
     2161 caactttact atttaatgag tcgtggtatt tctcaaagag aagcggaacg tcttgttata
     2221 catggtttct tagatccagt agtacgtgaa ttacctatcg aagacgttaa acgtcaattg
     2281 agagaagtaa ttgaacgcaa agtttctaaa taatattttg aaaataaaag tttgtaatag
     2341 atatagactg tcgatattgg tataagacta atacaacgtc agtatttaaa tgattaggat
     2401 ttttatttaa gaaaggtcgt gaatgaagtg gccgaacact catttgacgt taatgaagta
     2461 atcaaggatt ttccgatatt agatcaaaaa gtcaatggca aacgtttagc atatcttgat
     2521 tcaacagcga caagtcaaac gcctgtgcaa gtgttaaatg ttttagagga ttactacaag
     2581 cgttataatt caaacgttca tcgtggtgtt catacattag gatcattggc aactgatggt
     2641 tatgaaaatg cccgtgaaac cgttcgtcgt tttattaatg cgaagtattt tgaagaaatc
     2701 attttcacac gcggaacaac tgcgtcgatt aaccttgtag cacatagcta tggtgatgca
     2761 aatgttgaag agggcgatga aattgttgtc actgaaatgg aacatcatgc caatattgtt
     2821 ccttggcaac agttagcaaa gcgtaaaaat gcgacattga aatttatacc aatgacagct
     2881 gacggtgaat taaacatcga agatattaag caaacgatta atgataaaac aaagatcgtt
     2941 gctattgcac atatttctaa tgtactcggt acaattaatg atgttaaaac cattgcagaa
     3001 atagctcatc aacatggcgc aattatcagt gttgatgggg cgcaagcagc accacatatg
     3061 aaacttgata tgcaagaaat gaatgctgat ttttatagtt ttagtggtca taaaatgctt
     3121 ggaccaacag gtattggcgt attatttggt aaacgtgagt tactacaaaa aatggaaccg
     3181 attgagttcg gtggcgacat gattgatttt gtaagtaagt atgatgcaac atgggctgat
     3241 ttacctacta aatttgaggc gggtactcca ttaattgcac aagcaattgg gcttgcagaa
     3301 gctattcgct atttagaacg cataggtttt gatgcaattc ataaatatga acaagaatta
     3361 acgatatatg cttatgagca aatgtctgca attgaaggaa ttgaaattta tggcccgcca
     3421 aaggatcgtc gtgcaggtgt aataacgttt aatttacaag atgtacatcc acacgatgtt
     3481 gctacagccg tagatacaga aggtgtagcg gttagagctg ggcatcattg tgcgcaaccg
     3541 ttaatgaaat ggttaaatgt gtcttcaaca gctagagcga gtttttatat atacaacacg
     3601 aaagaagaca ttgatcagtt aataaatgcc ttgaaacaaa cgaaggagtt tttctcttat
     3661 gaattttaat aatctagatc aattatatag atctgtcatt atggatcatt ataaaaatcc
     3721 tagaaataaa ggtgtattag ataacgggtc tatgacagta gatatgaata acccgacatg
     3781 cggtgaccgt atacgactaa catttgatat agaagacggc attataaaag atgctaagtt
     3841 tgaaggtgaa ggttgttcga tttcaatggc aagtgcatcg atgatgacac aagctgttaa
     3901 aggtcattca cttggagaag caatgcaaat gagccaagaa tttacgaaaa tgatgcttgg
     3961 tgaagactat gtgattacag aagaaatggg agatattgaa gcattgcaag gtgtatctca
     4021 attcccagct cgtattaaat gtgccacatt agcttggaaa gcattggaaa aaggtactgt
     4081 tgctaaagaa ggtaaagcag aaggtacgac tgaagaagaa tagaatgctg ttaatcatag
     4141 atgattttga tattagacat ataaaagtat aaaaattttt ataagatgtc atgtcattgt
     4201 tataatatgg tttacatcat gaattaaaaa cttacgcacg ccgttgtaaa tatattttta
     4261 aggagtgatt gaaatggcta aaaaagcacc tgatgttggg gattataaat atggattcca
     4321 cgacgatgat gtgtccattt tcagatcaga acgtggttta actgagaata ccgttagaga
     4381 aatttctaac atgaaaaatg agccggaatg gatgttagat ttccgtctta aatcattaaa
     4441 attgttttat aaaatgccaa tgcctcaatg gggtggcgac ttatcagaat tgaatttcga
     4501 tgacattact tactatgtaa agccttcaga acaagctgaa cgttcatggg atgaagtgcc
     4561 agaagaaatc aaaagaactt tcgataaatt aggaattcct gaagctgaac aaaaatattt
     4621 agctggtgtt tctgctcaat atgaatctga agttgtttac cataatatgg aaaaagaact
     4681 tgaagaaaaa ggtattatct ttaaagatac agatagtgct ttacaagaaa atgaagaatt
     4741 attcaaaaaa tactttgctt ctgtagtacc tgcagcagat aacaaatttg cggcgttaaa
     4801 ctcagcggta tggtcaggtg gttcgttcat ttatgtacct aaaaatatca aactagatac
     4861 gccactacaa gcttatttcc gtattaactc tgagaacatg ggtcaatttg aacgtacatt
     4921 aatcattgct gatgaaggtg cttctgtaca ttacgtagaa ggttgtactg caccagttta
     4981 tacaactagt tctttacact ctgctgttgt ggaaatcatt gtgcataaag atgcgcacgt
     5041 tcgttatact acgattcaaa actgggcgaa caatgtatac aatttagtta caaaacgtac
     5101 ttttgtttat gaaaacggaa atatggaatg ggtagatggt aacttaggtt ctaagttaac
     5161 gatgaaatat ccaaactgtg ttcttttagg tgaaggtgca aaaggtagta cattatctat
     5221 tgcatttgct ggtaaaggac aagttcaaga tgccggtgct aaaatgattc ataaagcgcc
     5281 aaatacatct tcaacgattg tttctaaatc tatttctaaa aatggtggta aagttattta
     5341 tcgtggtatt gtacattttg gacgtaaagc aaaaggcgct cgttcaaata ttgaatgtga
     5401 tacattaatc ttagataacg aatcaacatc agatacaatt ccatataacg aagtattcaa
     5461 cgatcaaata tcattagaac atgaagccaa ggtttcaaaa gtttctgaag aacaattatt
     5521 ctatctaatg agtcgtggta tttctgaaga agaagcgaca gaaatgattg ttatgggatt
     5581 catcgaacca tttacaaaag aacttccaat ggaatacgcg gtcgaaatga accgtttaat
     5641 caagttcgaa atggaaggta gtattggcta gctttaaacc gcgttgttaa gctattctta
     5701 acttccggaa ataggtattg ataccatttc gatactgaat ataacaaaaa gccacattaa
     5761 tgtggctttt tttggtttta taactaaatc ggattgatag ataagctttg tgcgtattta
     5821 taaatccgat tttttgattg gtgtaaaaaa taatcattga tggtggataa agcgacaaca
     5881 caaatacaac atgattgtgg cattagagtg ctggtcttta ttaaattaat tgaaaactac
     5941 atcaaatatt ctttaaagat aattcgatat tagttcgatt aagattcgtt gtataagtga
     6001 gttaaaataa gaaaactatt aataatatta agttcactac agatgttgct aatggaccgt
     6061 aagttttaaa gacatcttta cttttataac caacaatcgc atctaaaaat tgaactaaaa
     6121 tcattgcaat ggatatagtt atcaaaaata tagcactatg aatgactaaa gaaaaaatag
     6181 ctaattaaaa taaaggtaag cttcgactaa gtgcataata tgcatttata ttatggctag
     6241 atgcacatgc ttgaattgaa taacctaaac ttatactggc actaatgatt gtaaatatag
     6301 ctaaaacaaa atacatgtta atccttcttt ctatatttgg atataaacaa gtacttgtct
     6361 aaagttattt aaaagataat tagaataaat ttatgagaaa cttgttgtta tcattatagc
     6421 ggtttcaaat cattataact atgtcataaa ctgaatttgt taaatttttt tattgtgtaa
     6481 atttattcgt aacaatcagg tcgaactata gcatcatttt actaatgaac gcattaaagt
     6541 aactatgact aaaaatgcat attaattatc attattaaga ctattctata taatgaattt
     6601 taactggttt attaaacgag aacgtcggga attaagtaac tacaataaaa ataagatatg
     6661 acaataagga gactacacgc gtgatcattg ccataattat attgatattt atttcgtttt
     6721 tcttttcagg aagcgagacg gcattaacgg ctgccaataa aacaaaattt aaaactgaag
     6781 ctgataaagg tgataaaaaa gcaaaaggca ttgtaaagtt acttgaaaaa ccaagcgagt
     6841 ttattacaac gattctaatt gggaataatg tcgcgaatat tttattacca acacttgtta
     6901 caattatggc tttacgttgg gggattagcg ttggtattgc atcagctgtt ttaacagttg
     6961 ttatcatttt gatttccgaa gtgattccca agtctgtcgc tgcaacattt ccagataaaa
     7021 taacaaggct tgtatatcca attattaata tttgtgtcat tgtgtttcgc cctatcacat
     7081 tacttttaaa taagttgacg gacagtatta atcgaagttt atctaagggc caacctcaag
     7141 aacatcaatt ttcaaaagaa gaatttaaaa caatgttagc aattgctgga catgaaggtg
     7201 ctttaaatga aattgagacg agtaggttgg aaggtgtcat taattttgaa aatttaaaag
     7261 taaaagatgt agatacaaca cctagaatta atgtgacggc atttgcttca aatgcgacat
     7321 acgaagaagt ttatgaaacg gttatgaata agccatacac tagatatcca gtatatgagg
     7381 gagatattga taacattatt ggggtgtttc attctaaata tctgttggct tggagtaata
     7441 aaaaagaaga tcaaattaca aactattcag ctaagccatt atttgtgaat gaacacaata
     7501 aagctgaatg ggtattacgt aagatgacta tttctagaaa acatttagca attgtgttgg
     7561 acgaatttgg tggtaccgaa gcgatagtgt cacatgaaga cttaattgaa gaattattag
     7621 gtatggaaat tgaagatgag atggataaaa aggaaaaaga aaaactttct caacagcaaa
     7681 ttcaatttca acaacggaaa aatcgcaacg tatctatata aggagcgaac agctatgtgg
     7741 aataagaatc gacttactca aatgttaagt attgaatatc caattataca agcaggtatg
     7801 gcaggaagta cgacaccgaa attagttgca tcagtaagta acagtggtgg gttaggcaca
     7861 ataggcgcag gttactttaa tacgcagcaa ttagaagacg aaatagatta tgtacgccaa
     7921 ttaacgtcaa attcttttgg cgtaaatgtc tttgtaccaa gtcaacaatc atataccagt
     7981 agtcaaattg aaaatatgaa tgcatggtta aaaccttatc gacgcgcatt acatttagaa
     8041 gagctggttg taaaaattac cgaagaacaa caatttaagt gtcatattga tacgataatt
     8101 aaaaagcaag tgactgtatg ttgttttact tttggaattc caaatgaatc tattataaaa
     8161 agattgaaag aagcaaacat taagctaata ggtacagcaa caagtgttga tgaagctatt
     8221 gcgaatgaaa aagcgggtat ggatgctatc gttgctcaag gtagtgaagc aggtggacat
     8281 cgtggttcat ttttaaaacc taaaaatcaa ttacctatga ttggaacaat atctttagtg
     8341 ccacaaattg tagatgtcgt ttcaattccg gtcattgccg ctggtggaat tatggacggt
     8401 agaggagttt tggcaagtat tgtcttaggt gcagaagggg tacaaatggg caccgcattt
     8461 ttaacatcac aagatagtaa tgcatcagaa ctactgcgag acgcaattat aaatagtaaa
     8521 gaaacagata cagtcgttac aaaagcgttt agtggaaagc ttgcacgcgg tatcaacaat
     8581 aggtttatcg aagaaatgtc ccaatacgaa ggcgacatcc cagattatcc aatacaaaat
     8641 gagctaacaa gtagcataag aaaagccgca gcaaacatcg gcgacaaaga gttaacacat
     8701 atgtggagtg gacaaagccc gcgactagca acaacgcatc ccgccaacac catcatgtcc
     8761 aatataatca atcaaattaa tcaaatcatg caatataaat aatcgaccgc aatccacaaa
     8821 agcacaagca cccccaaaca ttattttagt gcttgccatt tttgtggatt gcgtttctat
     8881 tttaccaatc taatcaaacg aaattatcga gctgaaggtc accgaaatga ttataattaa
     8941 acgaaattat cgagctaaag ttcgctgaaa tgattatgat taaacgaaat catcaaacga
     9001 aagttcactg aaatgattat gatcaaacga aatcatcata cgaaagttca ctgaaatgat
     9061 tataatcaaa cgaaaacatc aagctgaaga acgccgaaag attttaatca agcaaaaaca
     9121 tcaaacgaaa gttcgctgaa atgattatga taaaagttat atggtatgat gacattggtg
     9181 atatatatga taaacatcgg attaacaggt tggggtgatc actattcatt atatgaagat
     9241 ttagaacgcc aaaccgataa acttaaaaca tatgctggac attttccggt tgtcgaatta
     9301 gatgcgacat actatgcgat acaaccggaa agaaatatat tgaaatggat aaaagaaacg
     9361 cctgatacat ttgaatttgt ggtcaaaatt catcaagcac tcacattgca tgcagactac
     9421 aaaacatttg cagatacaag gcaagaacta tttgatcaat ttaagaatat gttagagccc
     9481 ttacatacac agaaaaaatt agcaatggta ttagttcaat ttccgccatg gtttgactgc
     9541 aatgcacaaa atatcaaata tattttgtat gtaagacagc aattacaagc atttccaatg
     9601 tgtgtagaat ttaggcatca atcatggttt agtgatgcat ttaaagaaca aacattggca
     9661 tttttaacag aacatcaaat cattcatgca gtagttgatg aaccacaagt gaaagatggc
     9721 agtgtacctt tagtcaatcg aatcacaaat gaaattgcgt ttgtacgtta tcatggacgt
     9781 aatcattacg gttggactaa gaaagatatg tcagatcaag aatggcgaga tgtacgctat
     9841 ttatatgatt ataatgagca agaattaata gacttggcac aaaaggcaca aatattagca
     9901 caaaaagcta agaaagttta cgtcatattt aacaataatt ctggtggtca tgcagcaaat
     9961 aatgccaaaa catatcagcg attattgaat atagaatatg aagggttagc accacaacaa
    10021 ttaaaattat tttaagaggc gacgactatg ttattaacaa ttacattatt agttttaatc
    10081 ggaggtttgt cagcgattat agggtcaatc gtaggcattg gaggcggtat tattatcgtt
    10141 ccaacaatgg tttacctcgg tgttgaacat ggattactac ataatattac aacacaagta
    10201 gcgataggga cgtcttcagt cattctaatt gtgacaggac tttcttcatc acttggatat
    10261 ttaaaaacaa aacaagttga tattaaaaat ggttccatct ttttatttgg attattacca
    10321 ggttcattgc ttgggtcatt cattagtaga tatttaacat ttgagtcatt taacttatat
    10381 tttggaatct ttttaatttt cgtagccatt ttattaatgg taagaaataa gattaaaccg
    10441 tttaaaatat ttgataaacc taagtatgaa aaaacttatg tagatgctaa aggtaaaaca
    10501 tatcattata gtgtaccgcc tttattcgct tttgttacta catttttaat tggtattttg
    10561 acaggtttat ttggtattgg aggtggcgca cttatgacgc cactaatgct tattgtattt
    10621 agatttccac ctcatgtagc tgttggaaca agtatgatga tgattttctt ttcaagtgtc
    10681 atgagttcta tagggcacat tgctcaaggt cacgtagctt ggggttatgc aatcatttta
    10741 attatttcta gttattttgg tgcgaaaatc ggtgtcaaag tgaatcaatc aattaagtca
    10801 gatacggtag taacattatt gagaacagta atgttgttaa tgggtatata tttaattatt
    10861 cgtgcgttga tttaatacaa ctttaaaagg aggacgtcaa tttgaggctt acaatttatc
    10921 atacgaacga tattcatagt catttacatg aatacgaacg cattaaagca tatatggcag
    10981 aacatcggcc acgacttaac catccttctt tatatgttga tctaggtgat catgtagatt
    11041 tatccgcacc tataactgaa gcaactttag gtaaaaagaa tgtggcatta ctaaatgaag
    11101 caaaatgtga tgttgcaaca atcggtaata atgaagggat gaccatttca cacgaagctt
    11161 taaatcacct ttacgacgaa gcaaaattta tagtgacatg tagcaatgtt atagatgaat
    11221 caggtcattt accaaataat atcgtttctt cttatattaa ggatatagac ggtgtgaaaa
    11281 tactattcgt tgcagcgaca gcacctttta ccccatttta tcgtgcgcta gattggattg
    11341 ttaccgatcc acttgaatct ataaaagaag aaattgaact tcaacgaggt aaatttgatg
    11401 tattaatcgt gctaagtcat tgtggcattt tcttcgatga aacattatgc caagaattgc
    11461 ctgaaattga tgtcattttt ggtagtcata cgcatcatta ttttgaacat ggtgaaatta
    11521 ataatggtgt actgatggcg gcagctggaa agtatggtaa ttatcttgga gaggttaatt
    11581 taacttttga ggcacataaa gtagtacata aaactgcaaa gattattcct ttagaaacat
    11641 tacctgaagt tgaaacttca tttgaagaag aaggaaaaac gttaatgtcc aattcagtaa
    11701 ttcaacatcc agtagtgctt aagcgtagta tgaatcacat aactgaagct gcatacttat
    11761 tagctcaaag tgtttgtgag tatacacatg cacaatgtgc catcatcaat gccggcttac
    11821 tcgttaaaga tattgtaaaa gatgaagtga cagaatatga cattcatcaa atgttaccgc
    11881 atccgattaa tatggtaagg gttagacttt ctggtgtgaa attaaaagag attatagcta
    11941 aaagtaataa acaagaatat atgtatgaac atgcacaagg tttgggtttc agagggaata
    12001 tatttggagg atatattctt tataatttag ggtacattca ttctacaggg cgttactatc
    12061 tgaatggaga agaaatcgaa gacgacaaag aatatgtatt aggtacgata gatatgtata
    12121 cgttcggtcg ttatttccca atattgaaag aattaccaaa agagtattta atgccagagt
    12181 ttttaagaga tatatttaaa gaaaaattat tggaatatta aaaagtaaga ttattggatt
    12241 ttcatttgtc atgaatttcg atataatgtt taaagataca cttaacagga gggtatgtgt
    12301 tgttatggcg acaaaaaacg aggaaatatt acgtaaaccg gattggttga aaataaaatt
    12361 aaataccaac gaaaactata caggacttaa gaagatgatg agggaaaaaa atcttaatac
    12421 tgtatgtgaa gaagctaaat gtcctaatat acatgaatgt tggggtgcac gtcgtacagc
    12481 gacatttatg attttaggtg ccgtatgtac aagagcttgt cgtttttgtg cggttaagac
    12541 aggtttacct aatgaacttg atttaaatga gcctgaacgt gtagctgaat cagttgaatt
    12601 aatgaatttg aaacacgttg ttatcactgc tgttgcgcgt gatgatttaa gagatgctgg
    12661 ttcaaatgtt tatgctgaga cagtacgtaa agttagagaa agaaatccat ttacaacgat
    12721 tgaaatttta ccatcagata tgggcgggga ctatgatgcg ttagaaacat taatggcgtc
    12781 aagacctgac attttaaacc ataatattga aactgttcgt cgcttaacac cgagagttcg
    12841 tgcgcgtgcg acttacgaca gaacattaga gtttttacgt cgttcaaaag aattacaacc
    12901 ggatatccca actaaatcaa gtattatggt tggattaggt gaaactatag aagaaattta
    12961 tgaaacgatg gatgatttac gtgcgaatga tgtagatatt ttaacgattg gtcaatattt
    13021 acaaccttca cgtaaacatt taaaggttca aaaatattac acgcctttag agtttggtaa
    13081 attaagaaaa gtggcaatgg ataaaggttt taaacattgc caagctggac ctttagtacg
    13141 tagttcttat catgcggatg agcaagtaaa tgaagctgct aaagaaaagc aacgccaagg
    13201 tgaggcacag ttaaatagtt aatatttaac cattaataag gcataaaggc ttagtttgta
    13261 caaaacgaac gtgtcataga agtaatcatt atttttatga aacactaaaa gtataaactg
    13321 agcctttcat tgtcatagat aggtgaagaa tttgataaaa gtagatcaac attactttga
    13381 attaatagaa aattatcgcg aatgttttaa tgaagaacaa tttattgcta ggtattcaga
    13441 tattttagat aaatatgatt acatagttgg tgactatggt tacgatcaat tacgattaaa
    13501 aggcttttac aaagattcta ataaaaaagc agagatgagt aaacgttttt caaatattca
    13561 agattacata tttgaatatt gtaactttgg ttgtccttac tttgtattaa gacatttgtc
    13621 taaacaagag gttaaaaagt taatagaaga agttcatccg tctgatgtga tagatgacga
    13681 caataaactt caagatgtga agattaagcc aaccattcaa gaaactgaac attaataaaa
    13741 cccttagcta gattgaaaat gggaatcatg caattcaagc atggacctgt aatctagtta
    13801 ggggttttta tctttaatga atgacttcat ttaaatactc agtaatttca tcgccttctt
    13861 cagcatttac acctaaaata tgagcgatat agccttcttc ttttaaatca tcagtaccga
    13921 taataccgaa tttatttgtt tgcatattaa gtacgagtgt cttaccataa tgcctatttg
    13981 tatggactaa cattaaatca tatcgactat gctcgccaac aaaaccaaca aactgaactt
    14041 gactctcttc gttgtcatca tataaataca tatcaatcat tttgtagcga ctccttttaa
    14101 aagtagtaaa gttagtataa cgacaaatga agtatactgc aaaattatga taatatataa
    14161 gcgagaggtg acaaggaatg tattttgtag acaaagataa actaactcag aaattagcct
    14221 atttacaagc attaactgat gattaccatg agagcaagca caatcattat gcatttgaac
    14281 gcattgctca aatgttgata gaatcatcgg tagatatagg gaatatgatt atcgatgcat
    14341 ttattttaag ggatcctggt aattataaag atgtgattga tatattagaa ctagaaaatg
    14401 ttattactaa agaaacacag caggcgatta ataaaactgt cgatattcgt aaacaattta
    14461 catatgatta cacagcctta gatattaagg ttatcatgcc aatgtttgat gacgcattac
    14521 cttattacaa acaatttatt acagaagtaa cgacattttt acatcaagaa aatgtaccag
    14581 taacagcttt tggtaaagga gaaaatcaat aatgaaacag tataaagcgt atttaatcga
    14641 tttagatggc acaatgtata tgggaacaga tgagattgat ggagcaaaac aattcatcga
    14701 ttatttaaat gtaaaaggca ttcctcattt atacgtaact aataattcaa cgaaaacacc
    14761 tgagcaagta actgaaaaat tacgtgaaat gcacattgat gctaaaccag aagaggttgt
    14821 aacgtcagcg ttagccactg ctgattatat ttcagaacaa tcaccaggag catcagtata
    14881 tatgttaggt ggtagtggtt taaatactgc gttaaccgaa gcgggacttg tcattaaaaa
    14941 tgacgagcat gttgattatg tagttattgg acttgacgaa caagttacat atgaaaagct
    15001 tgcgattgca acgttaggtg taagaaatgg agcaacattt atttctacaa atccagatgt
    15061 gtcaattcca aaagaacgag gatttttacc tgggaatggt gctattacaa gtgttgtaag
    15121 tgtatcgaca ggcgtatcgc cacaatttat tggtaaacca gaaccaatca ttatggttaa
    15181 agcattagaa attttaggat tagataaatc cgaagttgct atggtaggcg atttgtacga
    15241 taccgatatt atgtctggta ttaacgtagg tatggatacg attcatgtac aaacaggtgt
    15301 atctacgtta gaagatgtgc aaaataaaaa tgtgccacca acgtattctt ttaaagattt
    15361 aaatgaagca atagctgaat tagaaaaata tatgtagtca ttttataaag taggtgaatt
    15421 gatttggtaa aaatagttgt ttcgaggaaa attccagata aattttatca acaattaagt
    15481 aaacttggtg acgttgttat gtggcaaaaa tcattagtgc ctatgcctaa agatcaattt
    15541 gcgacagcac ttcgtgacgc agatgcttgt tttatcacat taagtgaaca gatcgatgca
    15601 gaaattttag cacaatcacc aaatttaaaa gtaattgcga atatggctgt aggatatgac
    15661 aacatcgatg ttgaaagtgc aacagcgaat aacgtggttg tcacgaatac accaaatgta
    15721 cttactgaaa caactgcaga attaggattt acattaatgc ttgctatagc acgccgtatt
    15781 gtagaagctg aaaaatatgt agaagcagat gcatggcaaa gctggggtcc ttatttattg
    15841 tcaggtaaag atgttttcaa ttcaactatt ggaatatatg gtatgggaga tattggtaaa
    15901 gcttttgcaa gaaggttgca agggtttaat actaatattc tttatcataa tcgatcaaga
    15961 cataaagatg cagaggcgga ctttaatgca acatatgttt cttttgaaaa gctgttagca
    16021 gaaagtgatt ttatcatctg tacagcgcca cttacaaaag aaacacatca taaatttaat
    16081 gctgaagcat ttgaacaaat gaaaaatgat gcaattttta ttaatatcgg tagagggcaa
    16141 attgtagatg aaacagcatt aatcgatgca ctagacaata aagaaatttt agcatgtggt
    16201 ttagatgtat tagcaaatga accgattgat catacacatc cattaatggg acgtgataat
    16261 gttctgatta caccacacat tggtagtgca tcagtaacaa cacgggacaa tatgattcaa
    16321 ttatgtatta ataatataga agcggttatg acaaatcagg taccacatac tccagtaaat
    16381 tgaaaataag tatggttttt aatcattggt aaaacaagca aagcaattgt taaatatgtt
    16441 acgcttgact tgcgaatgta tatatttgcg ctgatgataa ttcaataaca ttataaactc
    16501 gtcatattaa tgtgatgagt ttatttgatt t
//
LOCUS       DAHHOJ010000057        16275 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.057, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000057 DAHHOJ010000000
VERSION     DAHHOJ010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16275
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.057"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(331..978)
                     /locus_tag="NBH32_001588"
     CDS             complement(331..978)
                     /locus_tag="NBH32_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1515181.1"
                     /translation="MKFDNYIFDFDGTLADTKKCGEVATQSAFKACGLTEPSSKEITH
                     YMGIPIEESFLKLADRPLDEVALAKLIDTFRHTYQSIEKDYIYEFAGITEAITSLYNQ
                     GKKLFVVSSKKSDVLERNLSAIGLNHLITEAVGSDQVSAYKPNPEGIHTIVQRYNLNS
                     QQTVYIGDSTFDVEMAQHAGVPSAAVTWGAHDARSLLHSNPDFIINDPSEINTVL"
     gene            complement(1091..1639)
                     /gene="hxlB"
                     /locus_tag="NBH32_001589"
     CDS             complement(1091..1639)
                     /gene="hxlB"
                     /locus_tag="NBH32_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-3-hexuloisomerase"
                     /protein_id="HCD1515182.1"
                     /translation="MAEFSDYQLILDELKMTLSHVEADEFSTFASKILHAKHIFVAGK
                     GRSGFVANSFAMRLNQLGKQAHVVGESTTPAIKSNDVFVIISGSGSTEHLRLLADKAK
                     SVGADIVLITTNKDSAIGNLAGTNIVLPAGTKYDEQGSAQPLGSLFEQASQLFLDSVV
                     MGLMTEMNVTEQTMQQNHANLE"
     gene            complement(1641..2273)
                     /gene="hxlA"
                     /locus_tag="NBH32_001590"
     CDS             complement(1641..2273)
                     /gene="hxlA"
                     /locus_tag="NBH32_001590"
                     /EC_number="4.1.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019834869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hexulose-6-phosphate synthase"
                     /protein_id="HCD1515183.1"
                     /translation="MELQLAIDLLNKEDAAELANKVKDYVDIVEIGTPIIYNEGLPAV
                     KHMADNISNVKVLADMKIMDAADYEVSQAIKFGADVITILGVAEDASIKAAIEEAHKN
                     NKQLLVDMIAVQDLEKRAKELDEMGADYIAVHTGYDLQAEGQSPLESLRTVKSVIKNS
                     KVAVAGGIKPDTIKEIVAESPDLVIVGGGIANADDPVEAAKQCRAAIEGK"
     gene            complement(2350..3108)
                     /gene="nagB"
                     /locus_tag="NBH32_001591"
     CDS             complement(2350..3108)
                     /gene="nagB"
                     /locus_tag="NBH32_001591"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCD1515184.1"
                     /translation="MKVLNLGSKKQASFYVACELYKEMAFNQHCKLGLATGGTMTDLY
                     EQLVKLLNKNQLNVDNVSTFNLDEYVGLTASHPQSYHYYMDDMLFKQYPYFNRKNIHI
                     PNGDADDMNAEASKYNDVLEQQGQRDIQILGIGENGHIGFNEPGTPFDSVTHIVDLTE
                     STIKANSRYFKNEDDVPKQAISMGLANILQAKRIILLAFGEKKRAAITHLLNQEISVD
                     VPATLLHKHPNVEIYLDDEACPKNVAKIHVDEMD"
     gene            3384..3746
                     /locus_tag="NBH32_001592"
     CDS             3384..3746
                     /locus_tag="NBH32_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YojF family protein"
                     /protein_id="HCD1515185.1"
                     /translation="MLEPIKEQEVIDLLASFEHKPVYLHVETTNGAYANHFDQRVFNA
                     GTFLRNIQITYTHAQLKGGNKEPYRIGLKLSNGGWVYVQGLTHFEVNEHDEFLIAGFN
                     YEGQLAAALQISERPFNL"
     gene            3760..4425
                     /gene="bshB2"
                     /locus_tag="NBH32_001593"
     CDS             3760..4425
                     /gene="bshB2"
                     /locus_tag="NBH32_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol biosynthesis deacetylase BshB2"
                     /protein_id="HCD1515186.1"
                     /translation="MTDERHVLVIFPHPDDETFSSAGTLASYIQKGIPVTYACLTLGQ
                     MGRNLGNPPFATRESLPSIRERELEEACKVIGITDLRKMGLRDKTVEFEPYEHIDGMI
                     KSLIDDTNPSLIISFYPGYAVHPDHEATADAVIRTVERMPKEERPRLTLVAFSNDATE
                     ALGEPDIQNDITDFKELKIKAFEAHASQTGPFLKQLASPEIDGKQHSFLTVEPYWTYH
                     FKS"
     gene            4438..5316
                     /locus_tag="NBH32_001594"
     CDS             4438..5316
                     /locus_tag="NBH32_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE2"
                     /protein_id="HCD1515187.1"
                     /translation="MTEFDLSTREGRWKHFGSVDPIEGTKPTTKNEMTDLQSTHKDFL
                     FEIEEVGIKNLVYPVLVDQYQTAGTFSFSTSLTKDEKGINMSRIIESVEKHYDNGIEL
                     EFNTLYQVLRTLQTNMKQNAAGVDVSGKWFFDRYSPTTNIKAVGNADVTYGLAIDGDK
                     VTRKELTIEATVTTLCPCSKEISEYSAHNQRGVVTVKTYINKDQDIVDDYKNKILDAM
                     EANASSILYPILKRPDEKRVTERAYENPRFVEDLIRLIAADLVEFDWLDGFDIECRNE
                     ESIHQHDAFAKLKYRK"
     gene            5598..7088
                     /locus_tag="NBH32_001595"
     CDS             5598..7088
                     /locus_tag="NBH32_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1515188.1"
                     /translation="MNYFVGNSLGVNLTGIEKAIINRLNLFKEMGRPAQCVFLSWNRY
                     LYRNAQNYITSSDYINMYDFFQEATYLERNEPFDWLSYWTDECHYTLKHVENSHDFRI
                     YDQERFLMYAHFQDPKYRILDYVNHFDSQRRKVKRDFYDVRGFLSCSRILVDKQQTLC
                     EFFYNPEGDTKLEKYFSYKDGKPEVQKIIVYYANKQYFFNNETELGAFFIKQLYQHGD
                     LFFSDRNVYTAPIFNLTPESIPVVAVLHSTHIKNIDALDSSPFKNVYKAMFENLSRYR
                     AIIVSTEQQKLDVEKRINRTIPVVNIPVGYSETIDTPVQTLDQRSVKLISVARYSPEK
                     QLHQQIELIKRLVSYVPKIELHMYGFGSESKKLNELIQKYGLENHVYLRGFLSNLDQE
                     YSDAYLSLITSNMEGFSLALLESLAHGVPVISYDIKYGPNELITPDFNGYLITKNDED
                     ALFDKVKYVIDHPEVQQRLSKGSLAKAQQYSKASLIKQWDQFVRLI"
     gene            complement(7214..8686)
                     /locus_tag="NBH32_001596"
     CDS             complement(7214..8686)
                     /locus_tag="NBH32_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1515189.1"
                     /translation="MNYILGTILESKITGVEKAQINRLKLFKQHGISSKCVYVKWNPY
                     SYTYAKQHQIENDVFTMYDYFQKAINYKKTKQVNWIQYWEKSCRYTLKFVENSNDVRI
                     YDEEQFVMYAHFLDKQYHHLNYVNYFDHKRRKVKRELYDGRGFLSCSRILGEGQRIVL
                     ENYYTPNGEIVIQKYFDDIKGKNTLTKVILNEDQHQQFFDTEDELVQYFLHQLCKNND
                     QIILDRPHELGNVIAGLNQSIPVIVVLHSTHLADTGNGIKSFYKTVFNNLTRYKAIVV
                     STEQQCQDISQYIENKIPVINIPVGYVANLKYQFDINQKEKNHIISIARLVENKQIKH
                     QVEVIKQLVTKHPNIQLNIYGHGNGLSEYRQLVEDYHLSEHVKFHGFKTHINEEIAKA
                     ELMLSTSKMEGFGLAILESLSVGTPVISYDVDYGPSELIQDGFNGYLVPQGDINQMVE
                     KVDQLLNNTQKLQQFSINSIESAQQYNATTISTKWQNILN"
     gene            complement(8805..12830)
                     /gene="sdrD"
                     /locus_tag="NBH32_001597"
     CDS             complement(8805..12830)
                     /gene="sdrD"
                     /locus_tag="NBH32_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrD"
                     /protein_id="HCD1515190.1"
                     /translation="MLNRENKTAITRKGMVSNRLNKFSIRKYTVGTASILVGTTLIFG
                     LGNQEAKAAESTNKELNEATTSASDNQSSDKVDMQQLNQEDNTKNDNQKEMVSSQGNE
                     TTSNGNKLIEKESVQSTTGNKVEVSTAKSDEQASPKSTNEDLNTKQTISNQEALQPDL
                     QENKSVVNVQPTNEENKKVDAKTESTTLNVKSDAIKSNDETLVDNNSNSNNENNADII
                     LPKSTAPKRLNTRMRIAAVQPSSTEAKNVNDLITSNTTLTVVDADKNNKIVPAQDYLA
                     LKSQIKVDDKVKSGDYFTIKYSDTVQVYGLNPEDIKNIGDIKDPNNGETIATAKHDTA
                     NNLITYTFTDYVDRFNSVQMGINYSIYMDADTIPVSKNDVEFNVTIGNTTTKTTANIQ
                     YPDYVSRDNNSIGSAFTETVSHAGNAEDPGYYIQTVYVNPSEKTLTNAKLKVEAYHKD
                     YPDNVGQINKNVTKIKIYQAPKDYVLNKGYDVNTNQLIDVTEQFKDKITYGTNDSVNV
                     DFGSINNSYVVMVDTKFEFTTSESPTLVQMATLTSDGNRSVSTGNALGFTNNQSGGAG
                     QEVYKIGNYVWEDTNKNGIQELGEVGVKGVTVVAYDNKTNKEVGRTITDEKGGYLIPN
                     LPNGDYRVEFSNLPKGYEVTPSKQGNNEELDSNGLSSVITVNGKDNLSADLGIYKPKY
                     NLGDYVWEDTNKNGIQDQDEKGISGVTVTLKDENGNVLKTVTTDADGKYKFTDLGNGN
                     YKVEFTTPEGYTPTTVTSGSDIEKDSNGLTTTGVINGADNMTLDSGFYKTPKYNLGNY
                     VWEDTNKDGKQDSTEKGISGVTVTLKNENGEVLQTTKTDKDGKYQFTGLENGTYKVEF
                     ETPSGYTPTQVGSGTDEGIDSNGTSTTGVIKDKDNDTIDSGFYKPTYNLGDYVWEDTN
                     KNGVQDKDEKGISGVTVTLKDENDKVLKTVTTDENGKYQFTDLNNGTYKVEFETPSGY
                     TPTSVTSGNDTEKDSNGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKDVKVTLLNEKGEVIGTTTTDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYFEEDTSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDAGKHTPVKPMSTTKDHHNKAKALPETGSENNGSNNATLF
                     GGLFAALGSLLLFGRRKKQNK"
     gene            complement(13197..16052)
                     /gene="sdrC"
                     /locus_tag="NBH32_001598"
     CDS             complement(13197..16052)
                     /gene="sdrC"
                     /locus_tag="NBH32_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin SdrC"
                     /protein_id="HCD1515191.1"
                     /translation="MNNKKTATNRKGMIPNRLNKFSIRKYSVGTASILVGTTLIFGLS
                     GHEAKAAEHTNGELNQSKNETTGTSENKTTEKVDSRQLKDNTQTATADQPKVTMSDSA
                     TVKETSSNMQSPQNATANQSTTQTSNVTTNDKSSTTYSNETDKSNLTQAKDVSATPKT
                     TTIKPRTLNRMAVNTVAAPQQGTNVNDKVHFSNIDIAIDKGHLNKDTGKTEFWATSSD
                     VLKLKANYTIDDSVKEGDTFTFKYGQYFRPGSVRLPAQNQNLYNAQGNIIAKGIYDSK
                     TNTTTYTFTNYVDQYTNVSGSFEQVSFAKRENATTDKTAYKMEVTLGNDKYSKDVIVD
                     YGNKKGQQLISSTNYINNEDLSRNMTVYVNQPKNKYTKETLVTNLTGYKFNPDAKNFK
                     IYEVTNQNQFVDSFTPDTSKLTDVTDKFKITYSNDNKTATVDLLNGQSSSDKQYIIQQ
                     VAYPDNSSTDNGKIDYTLETQNGKSSWSNSYSNVNGSSTANGDQKKYNLGDYVWEDTN
                     KDGKQDANEKGIKGVYVILKDSNGKELDRTTTDENGKYQFTGLSNGTYSVEFSTPAGY
                     TPTTANAGTDDAVDSDGLTTTGVIKDADNMTLDSGFYKTPKYSLGDYVWYDSNKDGKQ
                     DSTEKGIKGVKVTLQNEKGEVIGTTETDENGKYRFDNLDSGKYKVIFEKPAGLTQTGT
                     NTTEDDKDADGGEVDVTITDHDDFTLDNGYYEEETSDSDSDSDSDSDSDSDSDSDSDS
                     DSDSYSDSDSDSDSDSDSDSDSDSDSDSDSDSDSYSDSDSYSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDRDSDSDRDSDSDSDSDSDSDSDSDS
                     DSDSDSDSDSDSDSDSDSDSDSDSDSDSDNDSDSDAGKHTPAKPMSTVKDQHKTAKAL
                     PETGSENNNSNNGTLFGGLFAALGSLLLFGRRKKQNK"
BASE COUNT         4848 a         2820 c         2466 g         6141 t
ORIGIN      
        1 ataataattc ttaaatcatc gaacgattaa ctttttggaa ttataactaa aaatagcgat
       61 agcacacaac ttcatgtaag tcatatgcta tcgccatttt cgacatcata tttaaatgtt
      121 tatattcgat ttacttcaat aactttgtct ttactttaat ttatttgtac aaaaataaat
      181 gatcaacgaa tcagacatca ctctttaaaa attcatgatg tttaataaat atttatttta
      241 aattcctaat ctaaagcgaa atatttctat gtataatcta atatttatga atatacattt
      301 taaccatggt attctctgtt ttaacaaatt ttataatact gtattaattt ctgatggatc
      361 attgattata aaatctggat ttgaatgaag taatgacctt gcatcatgtg ctccccatgt
      421 tactgctgca gatggtacac cagcatgttg cgccatctca acatcaaatg ttgaatcacc
      481 aatataaacc gtttgttggc tatttaaatt gtagcgttgc acaattgtgt gtatgccttc
      541 agggtttggt ttatatgcac ttacttgatc ggatccaaca gcttcggtaa tcaagtgatt
      601 taatccaata gccgataaat ttctttctaa tacatcactc ttcttactag acaccacgaa
      661 aagttttttc ccttggttat acaaacttgt aatggcttca gttatacccg caaattcata
      721 aatatagtcc ttttcaatag attgatatgt atgtctaaat gtatcgatta actttgctaa
      781 tgctacttca tctaatggtc ggtctgctaa ttttaaaaat gattcttcaa taggtattcc
      841 catataatgc gttatttctt tagatgatgg ttccgttaag ccacatgctt taaatgcact
      901 ttgtgttgct acttcaccac attttttcgt gtctgccaac gtaccatcaa aatcaaaaat
      961 ataattgtca aacttcaata ttaacacccc tattcgttta ttattgatat tatactataa
     1021 tttattattt aaataaaaaa ataatcgata atatttctgc ctaggcctca tattatcgac
     1081 tatctttgtt ttattctaaa ttagcatgat tttgttgcat cgtttgttcc gttacattca
     1141 tttcagtcat caatcccatt acaacactat ctaaaaataa ttgagatgct tgttcaaaca
     1201 aacttcctaa tggttgtgcc gagccttgtt catcatattt tgtacctgca ggcaaaacga
     1261 tgttcgtccc agctagattg cctattgcag aatctttatt tgtagtaatt aatacgatgt
     1321 cagcacctac tgattttgct ttgtctgcta ataatcttaa atgttccgtg gaacctgaac
     1381 cagagataat tacaaataca tcattcgact taatcgcagg tgtcgttgat tctccaacaa
     1441 catgtgcctg tttgccgagc tgatttaagc gcattgcaaa gctattcgcc acgaatcctg
     1501 aacgtccttt gccagctaca aatatatgtt tagcatgtag tattttggac gcaaacgttg
     1561 aaaattcatc cgcttcaaca tgtgacaaag tcatctttaa ttcatctaga attaattgat
     1621 agtcactaaa ttcagccata ttacttacct tcgattgcag cgcgacattg ttttgcagct
     1681 tctactggat catctgcatt tgcgattccg ccaccaacaa taacaagatc aggactttca
     1741 gcgacaattt ctttaattgt atctggttta attccacctg ctactgcaac tttagaattt
     1801 ttaataacag atttaacggt tcttaaactt tctaatggtg attgcccttc tgcttgtaaa
     1861 tcataaccag tgtgtactgc aatataatcc gcacccattt catctagttc ttttgcacgt
     1921 ttttctaaat cttgaacagc aatcatatca actagtaatt gtttattatt tttatgagct
     1981 tcttcaatag ctgctttaat tgatgcatct tctgcaacac ctagtattgt aattacatcc
     2041 gcgccaaatt taattgcttg gctaacttca taatcagctg catccataat tttcatgtct
     2101 gctaatactt ttacattact aatgttgtct gccatatgtt taactgctgg taaaccttcg
     2161 ttgtaaatga ttggcgtacc gatttctacg atatctacat aatcttttac tttatttgct
     2221 aactcagccg cgtcttcttt gtttaataaa tcaattgcta attgtaattc cactaaaaat
     2281 catccttcca atgcttttta tctttctatt ggaacctttc ccgaggcatt tcttaattaa
     2341 acattgcaat caatccattt catcgacatg aattttcgca acatttttcg ggcaagcttc
     2401 gtcgtctaaa tatatctcaa cattcgggtg tttgtgaagt aatgtggctg gaacatcaac
     2461 agaaatttcc tgatttaata aatgtgtaat agcagcacgt ttcttttcac caaatgcgag
     2521 taaaatgata cgtttggctt gaagaatatt agcaagtccc atcgaaatgg cttgctttgg
     2581 aacatcatct tcgtttttaa aatatcgact attagcctta atagtacttt cagtcaaatc
     2641 aacgatatga gtaacgctat caaacggcgt accaggttca ttaaatccaa tatgaccatt
     2701 ttctccaata cctaaaattt gaatatcacg ttgaccttgt tgttctaaaa cgtcattata
     2761 ttttgacgct tccgcattca tatcatcggc atctccattt ggaatatgaa tgttctttct
     2821 attaaaataa ggatattgtt tgaaaagcat gtcatccata taatagtgat aactttgcgg
     2881 atgtgatgcg gttaaaccta catattcgtc taaattaaac gtggatacat tatctacgtt
     2941 taactgattt ttatttaata acttaacaag ttgttcatac aaatctgtca ttgtaccacc
     3001 agttgctaaa cctagtttac agtgctgatt aaatgccatc tctttatata actcacatgc
     3061 aacatagaat gatgcttgtt ttttcgatcc taagtttaat actttcattg tttacatgcc
     3121 tccacaaatt taatacattt taaaaactta tgaattcatt catacaccat taatatagca
     3181 tcatttttaa tgtttaacac ttttcaaatt ccaacaaatt tttgattttt aaaagtaaaa
     3241 aacgaatgct gtatgattac gtaatcctga gtaattaccc ttcatgaaaa ttaatttata
     3301 aataattgag caatcgtata caattatcga atagttttta gtaaaataga aagataaaca
     3361 cattgatagt gtaaggagtg aacgtgttgg aaccaattaa agaacaagaa gttatagatt
     3421 tacttgcttc ttttgaacat aaacctgtgt atctgcatgt tgaaacgaca aatggggcct
     3481 atgcgaacca tttcgatcaa cgtgttttta atgctggtac ttttttaaga aacatacaaa
     3541 taacatatac gcatgcacaa ctaaaaggtg gtaataaaga accttatcgc attggtctta
     3601 aactatcaaa tggtggttgg gtttatgtac aaggtctaac gcattttgag gtcaatgaac
     3661 atgatgaatt tttaattgca ggttttaatt acgaaggtca actcgctgca gcacttcaaa
     3721 ttagtgagcg accatttaat ttatagagga ggcatcaaca tgactgacga aagacatgta
     3781 ttagtaatat ttccacatcc tgatgatgaa accttctcat ctgcaggcac attagcaagt
     3841 tatatccaaa aaggcatacc tgtaacatat gcatgtttaa ccctaggaca aatgggcaga
     3901 aatttaggta atcccccatt tgcaacacgc gaatcattac caagtattcg tgagcgtgaa
     3961 ctagaagaag cttgtaaagt tattggtatt actgatttaa gaaaaatggg tttacgtgac
     4021 aaaactgtcg aatttgaacc ctacgaacat atagatggta tgattaaatc tttaatcgat
     4081 gatacaaatc catcattgat tatttcattt tatcctggat atgcagtaca tcctgatcac
     4141 gaagcaacag cagacgctgt aattcgaacg gttgaacgca tgcctaaaga agaacgccct
     4201 cgtttaactt tagttgcgtt cagtaatgat gcaaccgaag ctcttggaga acctgatatt
     4261 caaaatgata ttacagattt taaagaactt aaaattaagg catttgaagc acatgcttct
     4321 caaacaggtc catttttaaa acaattagca agtcctgaaa tcgatggtaa acaacacagc
     4381 tttttaacag tcgaaccata ttggacatat cactttaaat cttaacggag gaaaaaaatg
     4441 actgaatttg atttatcgac acgagaaggt cgttggaaac actttggttc cgtagatcct
     4501 attgaaggca cgaaacctac tactaagaac gaaatgacag acttacaaag tacacataaa
     4561 gattttttat ttgaaataga agaagttggt attaaaaact tagtgtaccc tgtacttgtt
     4621 gaccagtatc aaacagctgg cacatttagt ttttcaacaa gtttaacaaa agacgaaaaa
     4681 ggtattaata tgagccgtat aattgaaagt gttgaaaagc actatgataa tggtattgaa
     4741 cttgaattta atacacttta tcaggtgtta cgcactttac aaacgaatat gaaacaaaat
     4801 gcggcaggcg ttgatgtttc tgggaaatgg ttctttgatc gctatagtcc aaccacaaat
     4861 attaaagcag ttggtaatgc cgatgtcact tatggattag caattgacgg tgataaagtt
     4921 acacgtaaag aacttactat tgaagcaact gtcacaacgc tatgcccttg ttcaaaagaa
     4981 ataagtgagt attcagcgca caaccaacgt ggtgttgtca ctgttaaaac atatattaat
     5041 aaagatcaag acatagttga tgactataaa aataaaattt tagatgcaat ggaagctaat
     5101 gcaagttcaa ttctataccc tattttaaaa cgtcctgatg aaaaacgtgt gacagagcgt
     5161 gcatatgaaa atccacgttt tgtcgaagac ttaattcgtt taattgccgc ggacttagtt
     5221 gaatttgatt ggttagacgg tttcgatatc gaatgtcgta atgaagagtc tatccatcaa
     5281 catgatgctt tcgctaaatt aaagtatcgc aaataatatc atatgattaa ttcaaacgcg
     5341 taacagtagt tgcgcgtttt tattatacct tctagagttg tatatctctt ttataaatct
     5401 tctcatttga tgtgcctgag tcataaatca atttcctaaa ctctacaatg atatattggt
     5461 cgtagttgac tgaataacta ctaccattat aaatatcaaa acttgttgtt ttataaataa
     5521 acacatcttc cagttaaaag aagtaattaa atattatgtg atataattat aaaaaataga
     5581 tataagtggt gttgtaaatg aattattttg taggtaatag tttaggtgtt aatttaactg
     5641 ggatagagaa agcaattatt aatcgtttaa atttatttaa agaaatggga agaccagcgc
     5701 aatgtgtatt tctgtcatgg aatagatatt tatatagaaa tgcccaaaat tatattacta
     5761 gttctgacta tattaatatg tatgatttct tccaagaagc aacatattta gaacgcaatg
     5821 aaccatttga ctggttgtcg tattggactg atgaatgtca ttatacttta aaacatgttg
     5881 aaaatagtca tgactttaga atatatgatc aagagcgttt cttaatgtat gcccattttc
     5941 aagatcctaa atatcgtata ttagattatg tgaatcattt tgatagtcaa agacgaaaag
     6001 taaaacgtga tttttatgat gtgcgtggct ttttaagttg tagtaggatt cttgttgata
     6061 aacaacaaac attatgtgaa tttttctata atccagaagg tgacacaaaa ttagaaaagt
     6121 atttttctta caaagatggc aaaccagaag tacaaaaaat tattgtttac tatgctaaca
     6181 aacaatattt ctttaataat gaaacggaat taggcgcatt tttcattaaa caactatatc
     6241 aacatgggga tttatttttc agtgaccgta atgtgtacac agcacctatt ttcaacttaa
     6301 cgcctgagtc aatcccagta gtagctgtgc ttcacagtac acacattaaa aatattgatg
     6361 cattagattc atcgccattt aaaaatgtat ataaagcgat gtttgaaaat ttatctcgat
     6421 atcgtgcaat tatcgtttct actgagcagc aaaaattaga tgttgaaaaa cgtattaacc
     6481 gtactattcc agttgttaat attcctgtcg gctatagtga aacaattgat acaccagtcc
     6541 aaacattaga tcagcgcagt gttaaattaa tttccgttgc tcgttattca cctgaaaaac
     6601 aattacatca acaaattgaa ttaattaaac gcttagtatc gtatgtccca aaaatagagc
     6661 ttcatatgta tggttttggt tctgaaagta aaaaattaaa tgaattgata caaaaatatg
     6721 gattggaaaa tcatgtttac ttacgcggct ttttaagcaa tctagaccaa gaatatagcg
     6781 atgcctattt gagtttaata acaagtaaca tggaaggttt ctctttagcg ctacttgaat
     6841 ctttagcaca tggtgttcca gttattagtt atgatattaa atatggacct aacgaattaa
     6901 ttacgcctga tttcaatggt tatttaatta ctaaaaatga tgaagatgcc ctatttgata
     6961 aagtaaaata tgtgattgat catcctgaag tacagcaacg actgtcaaaa ggtagtttgg
     7021 caaaagcgca acaatatagt aaagcgtcgt taattaaaca atgggaccaa tttgttcgtc
     7081 ttatataaat gtacttcacc tttattttta taaaaaaaaa gcgttttcct atgtcattta
     7141 actaaacatg taagttcgta tgaacgttgt ttgttaaata gatgattcta ggaaaatgct
     7201 tttttctttt gacttagttt aaaatatttt gccactttgt actgatagta gttgcattgt
     7261 actgttgtgc agattctatg ctattaatcg aaaactgttg caatttttga gtattattta
     7321 gtaattggtc gaccttttca accatttgat tgatgtcacc ttgaggtact aaatagccat
     7381 taaatccatc ttgaatcagt tctgatggac cataatctac atcataactg atcactggtg
     7441 tacctactga aagcgactct aaaattgcta agccaaaacc ttccatttta cttgtcgata
     7501 acatcagttc tgctttagca atctcttcat taatatgcgt cttaaaacca tgaaatttta
     7561 catgttccga taaatgataa tcttctacaa gttgtcgata ttctgacaaa ccatttccat
     7621 gtccataaat atttaattga atattgggat gttttgttac taattgcttg atcacttcaa
     7681 cttgatgttt aatttgttta ttttcaacga ggcgagcaat tgatatgata tgatttttct
     7741 ccttttgatt gatgtcaaat tgatacttta aatttgccac gtagccaacc ggaatattga
     7801 taactggtat tttattttca atatattgtg aaatatcttg gcattgctgt tctgttgata
     7861 caacaatcgc tttataacgt gttaaattat taaacactgt tttataaaaa ctttttatac
     7921 cattaccggt gtcggctaaa tgtgtactgt ggagcacaac aataactgga atactttgat
     7981 ttaatcccgc tataacattt cctaattcat gaggacgatc taatatgatt tgatcattat
     8041 ttttacataa ttgatggaga aaatattgaa ctaattcatc ttctgtatca aaaaattgtt
     8101 gatgctgatc ttcatttaag ataacctttg tgagcgtgtt tttccctttt atatcgtcga
     8161 aatatttttg gatgacgatt tccccattag gtgtatagta attttcgagt acaatccgtt
     8221 gtccttcacc taaaattcga gaacaactta aaaagcctct tccatcatac aattcgcgtt
     8281 ttacttttct tcttttatga tcaaaataat tcacatagtt taaatgatga tactgtttat
     8341 ctaaaaaatg tgcatacatt acaaattgct cttcatcata tattctgaca tcatttgaat
     8401 tttccacaaa tttcaatgtg tacctacatg acttttccca atactgtatc cagttaactt
     8461 gctttgtctt tttataattg attgcttttt gaaaatagtc atacattgta aatacatcat
     8521 tttcaatctg atgttgcttc gcatatgtgt atgaataagg attccattta acatatacac
     8581 attttgaaga tatgccgtgt tgtttgaaca acttcaatct atttatttgc gctttttcta
     8641 cacctgtaat tttactttct aaaattgttc ctaaaatgta attcatatta tcgcctcata
     8701 taagttttat tccgtatctt tattgtttat tttatatgaa aaatacatct attgcatgtg
     8761 taattataaa aaaaccaggc cacaaggacc tgggtcatat tgtattattt gttttgtttt
     8821 ttgcgacgac cgaataacaa taatgaacct aatgctgcaa ataatccacc aaataacgtt
     8881 gcgttatttg agccgttatt ttcactacct gtttctggta atgcttttgc tttattgtga
     8941 tggtctttag tagtactcat tggtttaaca ggtgtatgtt ttcctgcatc cgagtccgaa
     9001 tcgctatctg agtctgagtc gctgtccgaa tctgagtcgc tatctgagtc tgagtcgctg
     9061 tccgaatctg agtcgctgtc tgaatctgag tcgctatctg agtctgaatc actgtccgaa
     9121 tctgagtcgc tatctgagtc tgagtcgcta tctgaatctg aatcgctgtc tgagtctgag
     9181 tcactgtctg agtctgagtc gctatctgag tctgagtcac tgtctgaatc tgagtcgcta
     9241 tctgagtctg aatcgctgtc tgaatctgag tcgctatctg agtctgaatc gctgtccgaa
     9301 tctgagtcgc tatctgagtc tgagtcgcta tctgaatctg aatcactatc tgagtctgag
     9361 tcgctgtctg aatctgaatc gctgtctgag tctgaatcgc tatctgagtc tgaatcgcta
     9421 tctgagtctg aatcgctgtc tgagtctgaa tcactgtctg atgtatcttc ttcgaagtat
     9481 ccgttatcaa gtgtgaaatc atcatgatcc gtaattgtta cgtcaacttc gccaccatct
     9541 gcatctttat catcttcagt tgtatttgta cctgtttgtg ttaagccagc aggcttttca
     9601 aaaataactt tgtatttacc gctatctaaa ttatcgaaac gatatttacc attttcatct
     9661 gttgtagttg ttccaattac ttcgcctttt tcatttaata aagtaacttt aacatctttg
     9721 ataccttttt cagttgaatc ttgtttgccg tctttattac tgtcgtacca aacataatca
     9781 cctaaactat attttggtgt tttatagaaa ccactgtcta atgtcatgtt atctgcatct
     9841 ttaatgacac ctgttgttgt taaaccatta gaatcttttt cagtatcatt tccagaagtt
     9901 actgaagttg gtgtataacc tgatggtgtc tcgaattcaa ctttataagt tccattgttt
     9961 aaatcagtga attgatattt accattttca tctgttgtaa ctgtttttaa aactttgtcg
    10021 ttttcatctt ttaacgtaac tgttacacct gaaataccct tttcatcttt atcttgaaca
    10081 ccgtttttat ttgtatcttc ccatacatag tcacctaagt tgtaagtcgg tttgtagaaa
    10141 ccagagtcaa tagtatcgtt atctttatct ttaatgacac ctgttgttga tgtaccattt
    10201 gaatctatac cttcatcagt tcctgaacct acttgtgttg gtgtgtaacc tgatggtgtt
    10261 tcgaattcaa ctttataagt tccattttct aatccagtaa attgatattt accatcttta
    10321 tctgttttag ttgtttgtaa aacttcaccg ttttcatttt tcaatgtaac tgttacgcct
    10381 gaaatacctt tttcagttga atcctgctta ccatctttat ttgtatcttc ccatacataa
    10441 ttacctaaat tatattttgg tgttttgtaa aatccactat ctaatgtcat gttatcagca
    10501 ccattaataa cacctgttgt tgttaaacca ttagagtctt tttcaatgtc gctaccagat
    10561 gttactgtag tcggtgtata gccttctggt gtagtaaatt caactttata attaccatta
    10621 cctaaatcag taaatttata tttgccatca gcgtctgttg taactgtttt taacacgtta
    10681 ccgttttcat cttttaatgt taccgttacg ccagatatac ctttttcatc ttgatcttga
    10741 ataccatttt tatttgtatc ttcccataca tagtcaccta agttgtattt aggtttgtaa
    10801 atacctaagt ctgcagataa gttatctttg ccattaactg taataactga agataagccg
    10861 tttgaatcta attcttcgtt attaccttgt tttgaagggg ttacttcata accttttggt
    10921 aagtttgaaa attctacacg gtaatctcca ttaggtaagt ttggaatcaa gtaccctcct
    10981 ttttcatcag taatcgttct tccaacttct ttattagttt tattgtcata tgcaaccact
    11041 gttacacctt ttactccaac ttcccctaat tcttgaatac catttttatt tgtatcttcc
    11101 caaacgtagt taccaatttt atagacttct tgaccagcac caccactttg gttattagta
    11161 aatcctaaag cattgccagt agaaacggat ctgtttccat cagaagtcaa agttgccatt
    11221 tgaacaagtg ttggactttc actagttgta aattcaaatt ttgtatccac cataactaca
    11281 taagaattgt taatgctacc aaaatcaaca tttacactgt cattagttcc gtatgtaatc
    11341 ttatctttaa attgttcagt cacatctata agttgatttg tattaacatc ataacctttg
    11401 tttaaaacat agtcttttgg tgcttgataa attttaattt tagttacatt tttatttatt
    11461 tgacctacat tgtcaggata atccttatga taagcttcaa cttttaattt agcatttgtt
    11521 aaagtttttt ccgatggatt tacataaaca gtctgtatat agtaacccgg gtcttctgca
    11581 ttacctgcat gtgaaacagt ttcagtaaat gctgaaccta tagaattatt atctctagaa
    11641 acataatctg gatattgaat gttagcagtt gtttttgttg tagtattacc tatcgtaaca
    11701 ttaaactcaa catcgttttt actaacagga attgtatcag catccatata aattgaataa
    11761 ttaattccca tttgtacaga attaaatcta tctacataat ctgtaaatgt atatgtaatt
    11821 aaattatttg cagtatcatg ttttgcagtc gcaattgttt caccattatt tggatcttta
    11881 atatctccaa tatttttaat atcttccgga ttcaatccat atacttgtac tgtatctgag
    11941 tattttattg tgaaataatc acctgattta actttgtcat caactttaat ttgtgatttt
    12001 aatgctagat aatcttgtgc tggtacgatt ttattgtttt tatctgcatc aacgacagtt
    12061 aatgttgtat ttgatgtgat taaatcatta acatttttag cctctgttga tgatggctgt
    12121 actgctgcta tacgcattct tgtattcaaa cgtttaggtg ctgtactttt tggcaaaatg
    12181 atatctgcat tattttcatt atttgaatta ctattgttat caacaagagt ttcatcatta
    12241 ctcttgatag catcactttt aacatttaat gtagttgatt cagttttggc atctaccttt
    12301 ttgttttcct cattagttgg ttgaacattt accactgatt tattctcttg caaatcaggt
    12361 tgtaacgctt cttgattact tatagtttgt ttagtgttta aatcttcatt cgtagatttt
    12421 ggtgaagctt gctcatctga tttggcagtt gaaacttcaa ctttatttcc agtggtagat
    12481 tgtacacttt ctttttctat taatttattc ccatttgaag tcgtttcatt accttgagat
    12541 gataccattt ctttttgatt atcattttta gtattgtctt cttgatttag ttgctgcata
    12601 tcaactttat cactcgattg attatcactt gctgaagttg tcgcttcgtt caattcttta
    12661 ttagtacttt ctgcagcctt tgcttcttgg ttccccagac caaaaattaa tgttgtacct
    12721 actaaaattg atgctgttcc cactgtgtac tttctaatcg aaaatttatt taatcgattg
    12781 gataccatgc cttttcttgt tattgccgtt ttattttctc tgtttagcat tagattactc
    12841 ctaattcatc aaatttttaa ataatacaat tgttttaaat acaaaaatgt atatcaatat
    12901 agtattacat ttttagataa agcacaatac tttaattatt tttctttatc gtaaaacgtt
    12961 atttaacatt cgtgtttaaa tgaaaatttt tatgagtttt gtaatcttta tttaattatc
    13021 ataaaaaata gtattatttg cccttaaaat taatatctta gcttttctaa ttcatagaca
    13081 attacatttc tgtaacaaat taaattgtat ctattcctta aagatttttt gttttatatc
    13141 tgggaatttc taaacagaaa aaaccaggcc acatggacct ggttaagtta atcatattat
    13201 ttattttgtt ttttacgacg accgaataac aataatgatc ctaatgccgc gaataatcca
    13261 ccgaataatg tgccattatt tgaattatta ttttcactac ctgtttctgg taatgcttta
    13321 gctgttttat gctgatcttt aaccgtactc attggtttag ccggagtatg tttacctgca
    13381 tctgaatctg aatcgttgtc tgagtccgaa tcgctatctg aatctgagtc gctgtctgaa
    13441 tctgagtcgc tgtctgaatc tgagtcgctg tctgaatctg aatcgctatc cgagtctgag
    13501 tcgctatctg agtctgagtc gctatctgaa tctgaatctc tgtctgaatc tgaatctctg
    13561 tctgagtctg agtcgctatc tgaatctgag tcgctgtctg aatctgaatc gctatctgaa
    13621 tctgaatcac tgtctgagtc tgagtcgctg tctgaatctg aatcgctatc tgaatctgag
    13681 tcgctgtctg aatctgagtc gctatctgag tatgagtcgc tatctgagta tgaatcgctg
    13741 tccgaatctg agtcgctatc tgagtctgag tcgctatctg agtctgaatc gctgtctgaa
    13801 tctgagtcgc tatctgagta tgaatcgctg tccgaatctg agtcgctatc tgagtctgag
    13861 tcgctgtccg aatctgagtc gctatctgag tcgctatctg atgtttcttc ttcgtagtaa
    13921 ccattatcaa gtgtgaaatc atcatgatcc gtaattgtta catcaacttc gccaccatcg
    13981 gcatctttat catcttcagt tgtatttgta cctgtttgag ttaagccagc aggtttttca
    14041 aagataactt tgtatttacc actatctaaa ttatcaaagc ggtatttacc attttcatct
    14101 gtttcagttg taccaattac ttcgcctttt tcgttttgca aagtaacttt aacaccttta
    14161 attccttttt cagtcgaatc ttgtttacca tctttattac tgtcgtacca aacataatca
    14221 cctaaactat attttggcgt tttgtagaat ccactatcta atgtcatgtt gtcagcgtct
    14281 ttaatgacac ctgttgtagt tagtccatca gaatctacag catcatctgt acctgcattt
    14341 gcagttgtcg gtgtataacc ggctggtgtt gaaaactcta cactataagt tccattgctt
    14401 aaaccagtga actgatattt accattttca tctgttgtcg tacgatctaa ttctttaccg
    14461 ttactatctt taagaatgac ataaacacct ttaatccctt tttcattggc atcttgttta
    14521 ccatctttat ttgtatcttc ccatacatag tcacctagat tatatttctt ttggtcgcca
    14581 ttagcagttg atgagccatt cacatttgaa taactgtttg accaactact ttttccattt
    14641 tgtgtttcta aagtataatc aattttccca ttatctgttg aactattatc tggataagca
    14701 acttgttgaa tgatgtattg tttatcacta ctagattgac cattcaataa atctactgtc
    14761 gccgtcttat tatcattact gtatgtaatt ttaaatttat cagtgacatc tgtaagtttt
    14821 gaagtatctg gggtgaaact atccacaaac tgattttggt tagttacttc ataaatttta
    14881 aagtttttag catctggatt aaatttataa ccagttaaat ttgttactaa tgtttctttt
    14941 gtatatttat ttttaggttg gtttacataa acagtcatat tacgtgataa atcttcattg
    15001 ttaatatagt ttgtacttga aatgagttgt tgtccttttt tattaccata atcgacaatg
    15061 acgtctttac tatatttatc gtttcctaat gtaacttcca ttttataagc agttttatca
    15121 gttgttgcat tttcacgttt ggcgaatgaa acttgttcaa agctaccgct aacatttgta
    15181 tattgatcta cataattcgt aaaagtatac gttgttgtat ttgttttact atcgtaaata
    15241 ccttttgcaa taatattacc ttgggcatta tataaatttt gattttgtgc aggtaatctt
    15301 acagaacctg gacggaaata ttgaccatat ttaaaagtaa atgtatcgcc ctctttaaca
    15361 gaatcatcga ttgtgtaatt cgcttttaat tttaaaacat cacttgaagt tgcccaaaat
    15421 tcagttttgc cagtatcttt atttaagtgt cctttatcaa tagcaatatc aatatttgaa
    15481 aaatgaactt tatcattaac atttgtacct tgttgtggtg ctgctactgt gttcacagcc
    15541 atacggttta atgttcttgg tttaatagtc gttgttttag gagtagctga aacatcttta
    15601 gcctgtgtta aattactttt atcagtttca ttactatatg tagttgatga tttatcattt
    15661 gttgttacat tgctagtttg tgtagtagat tgattagctg tagcgttttg tggtgattgc
    15721 atgttactac tagtttcttt aactgttgca ctatcactca ttgtcacttt aggctgatct
    15781 gcagttgcag tttgcgtatt gtcttttagt tgacgactat caactttttc agttgtttta
    15841 ttctcacttg tgcctgtcgt ttcatttttt gattgattta attctccatt cgtatgttct
    15901 gccgctttag cttcatgacc acttaaccca aaaatcaatg ttgtccctac taaaattgaa
    15961 gcagtaccta cagaatactt tcttatcgaa aatttgttta atcgatttgg tatcatgcct
    16021 tttctatttg ttgctgtctt tttattattc atttaataat actcctttaa aatatcaaaa
    16081 tttgataaat ataatatttt gcatatataa atttctgcat tactatcgta atataaattt
    16141 caataaagtg caatacttta attatttttc tttattttac aatgttattt gctacaaata
    16201 ttaatatgaa ttccagttta agaattgcaa tttttaatac tttttttctt taattgcttt
    16261 aaattattta tctat
//
LOCUS       DAHHOJ010000058        16057 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.058, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000058 DAHHOJ010000000
VERSION     DAHHOJ010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 16057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.058"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            208..2148
                     /gene="ltaS"
                     /locus_tag="NBH32_001599"
     CDS             208..2148
                     /gene="ltaS"
                     /locus_tag="NBH32_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyglycerol-phosphate lipoteichoic acid
                     synthase LtaS"
                     /protein_id="HCD1515192.1"
                     /translation="MSSQKKKISLFAFFLLTVITITLKTYFSYYVDFSLGVKGLVQNL
                     ILLMNPYSLVALVLSVFLFFKGKKAFWFMFIGGFLLTFLLYANVVYFRFFSDFLTFST
                     LNQVGNVESMGGAVSASFKWYDFVYFIDTLVYLFILIFKTKWLDTKAFSKKFVPVVMA
                     ASVALFFLNLAFAETDRPELLTRTFDHKYLVKYLGPYNFTVYDGVKTIENNQQKALAS
                     EDDLTKVLNYTKQRQTEPNPEYYGVAKKKNIIKIHLESFQTFLINKKVNGKEVTPFLN
                     KLSSGKEQFTYFPNFFHQTGQGKTSDSEFTMDNSLYGLPQGSAFSLKGDNTYQSLPAI
                     LDQKQGYKSDVMHGDYKTFWNRDQVYKHFGIDKFYDATYYDMSDKNVVNLGLKDKIFF
                     KDSANYQAKMKSPFYSHLITLTNHYPFTLDEKDATIEKSNTGDATVDGYIQTARYLDE
                     ALEEYINDLKKKGLYDNSVIMIYGDHYGISENHNNAMEKLLGEKITPAKFTDLNRTGF
                     WIKIPGKSGGINNEYAGQVDVMPTILHLAGIDTKNYLMFGTDLFSKGHNQVVPFRNGD
                     FITKDYKYVNGKIYSNKNNELITTQPADFEKNKKQVEKDLEMSDNVLNGDLFRFYKNP
                     DFKKVNPSKYKYETGPKANSKK"
     gene            2419..4302
                     /locus_tag="NBH32_001600"
     CDS             2419..4302
                     /locus_tag="NBH32_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HCD1515193.1"
                     /translation="MGMEAYKIEHLNKSYADKIIFDNLDLSISEGEKIGLVGINGTGK
                     STLLKVIGGIDDDFTANVMHPNQYRIRYSSQKQDLNEDMTVFDAVLSSDTTTLRIIKQ
                     YEQAVQAYADDQSDKLFKRMMDAQDAMDQHDAWDYNAEIKTILSKLGIHDTTKYIKEL
                     SGGQQKRVVLAKTLIEQPDLLLLDEPTNHLDFESISWLINYVKQYPHTVLFVTHDRYF
                     LNEVSTRIIELNRGKLASYPGNYESYIEMRAEREVTLQKQQQKQRALYKEELAWMRAG
                     AKARTTKQQARINRFNDLENEVNQQYKDDKGELNLAYSRLGKQVFELEDLSKAINDKV
                     LFEHLTEIIQKGERIGVVGPNGAGKTTLLNILSGEDQQFEGKLKTGQTVKIAYFKQTD
                     ETLDRDIRMIDYLREESEIAKEKDGTSVSITQLLERFLFPSATHGKKVYKLSGGEQKR
                     LYLLRLLVHQPNVLLLDEPTNDLDTETLTILEDYIHTFGGTVITVSHDRYFLNKVAQS
                     YWFIHDGQMEKIIGTFEDYESYKKSLDKNKSTLKQQSKSSTTVRKKNGLSYKEKLEYE
                     QLMKRIEQSEVRMEEIDVLMIEASADYGKIKELNEEKEQLEIQYDLDITRWSELEEIK
                     EQQ"
     gene            4314..6095
                     /gene="recQ"
                     /locus_tag="NBH32_001601"
     CDS             4314..6095
                     /gene="recQ"
                     /locus_tag="NBH32_001601"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HCD1515194.1"
                     /translation="MMQQTLSHYFGYETFRPGQEEIISKVLDHRNVLGVLPTGGGKSI
                     CYQVPGLLLGGTTIVISPLISLMKDQVDQLKAMGIQAAFLNSSLTQKEQQHIEKELTS
                     GNIQFLYVAPERFENRYFLNLLQRIKIHLVAFDEAHCISKWGHDFRPSYQNVISKVFM
                     LPQDFTIIALTATATVEVQQDIREKLNIAQTDQIKTSTKRRNLIFKVNPTYQRQKFIL
                     DYIKTHDEDAGIIYCSTRKQVEELQEALESQNIESVIYHAGLSNKEREEAQNDFLFDR
                     VKVVVATNAFGMGIDKSNVRFVIHYNMPGDLESYYQEAGRAGRDGLKSECILLFSERD
                     INLHEYFITVSQADDDYKDKMGEKLTKMIQYTKTKKCLEATIVHYFEPNEKLEECEQC
                     SNCVQQDKSYNMTQEAKMIISCIARMKQQESYSVIIQVLRGESTDYIKYKGYDQISTH
                     GLMKGYTTSELSHLIDELRFKGFLNENDEILMCDTSIKKLLSNEVEVFTTPFKQKATE
                     KVFINTVEGVDRALFSQLVEVRKKLSDKLTIAPVSIFSDYTLEEFAKRKPASKQDMIN
                     IDGVGSYKLKHYCPAFLETIQNYKAKV"
     gene            6316..7293
                     /locus_tag="NBH32_001602"
     CDS             6316..7293
                     /locus_tag="NBH32_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515195.1"
                     /translation="MIKFKNVTKRYGKHVAVDNISFNINEGEFFVLIGPSGCGKTTTL
                     KMINRLIHLSEGYIYFKDKPISDYPVYEMRWDIGYVLQQIALFPHMTIKENIAQVPQM
                     KKWKEKDIDKRVDELLEMVGLEPEKYKNRKPDELSGGQRQRVGVIRALAADPPVILMD
                     EPFSALDPISREKLQDDLIELQTKIKKTIIFVTHDIQEAMKLGDKICLLNEGHIEQID
                     TPEGFKNNPQSEFVKQFMGSHLEDDAPCVEENAIIRDLDIMKPIDEVTSMSAYPIVYD
                     NQPIEVLYQLLSESERVIVMQEDSVGQYVIDRKDIFKYLSQKKEVAQHD"
     gene            7286..8800
                     /locus_tag="NBH32_001603"
     CDS             7286..8800
                     /locus_tag="NBH32_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HCD1515196.1"
                     /translation="MTNFFDILSERKGQLFSTMIEHIQISFIALLIATAIAVPLGILL
                     TKTKTISEIVMNIAAILQTIPSLALLGLMIPLFGIGRVPAIIALVVYALLPILRNTYT
                     GIKEVDPSLIEAAKGIGMKPFRRLTKVELPIAMPVIMAGVRTAMVLIIGTATLAALIG
                     AGGLGDLILLGIDRNNASLILLGAIPAALLAIIFDLILRFMAKLSYKKLLMTLGVIVM
                     IIILAIAIPMFAQKGDKITLAGKLGSEPSIITNMYKILIEEETKNTVEVKDGMGKTAF
                     LFNALKSDDIDGYLEFTGTVLGELTKEPLKSKEEKKVYEQAKQSLEKKYQMTMLKPMK
                     YNNTYALAVKRDFAKQHNIRTIGDLNKVKDQLKPGFTLEFNDRPDGYKAVQKAYNLNL
                     DNIRTMEPKLRYQAINKGNINLIDAYSTDAELKQYDMVVLKDDKHVFPPYQGAPLFKE
                     SFLKKHPEIKKPLNKLENKISDEDMQVMNYKVTVKNEDPYTVAKDYLKAKGLIK"
     gene            9040..10095
                     /gene="hisC"
                     /locus_tag="NBH32_001604"
     CDS             9040..10095
                     /gene="hisC"
                     /locus_tag="NBH32_001604"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCD1515197.1"
                     /translation="MKEQLNQLSAYQPGLSPRALKEKYGIEGDLYKLASNENLYGPSP
                     KVKEAISAHLDELYYYPETGSPTLKAAISKHLNVDQSRILFGAGLDEVILMISRAVLT
                     PGDTIVTSEATFGQYYHNAIVESANVIQVPLKDGGFDLEGILKEVNEDTSLVWLCNPN
                     NPTGTYFNHESLDSFLSQVPPHVPVIIDEAYFEFVTAEDYPDTLALQQKYDNAFLLRT
                     FSKAYGLAGLRVGYVVASEHAIEKWNIIRPPFNVTRISEYAAVAALEDQQYLKEVTHK
                     NSVERERFYQLPQSEHFLPSQTNFIFVKTKRVNELYEALLNVGCITRPFPTGVRITIG
                     FKEQNDKMLEVLSNFKY"
     gene            10256..10798
                     /locus_tag="NBH32_001605"
     CDS             10256..10798
                     /locus_tag="NBH32_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'(3')-deoxyribonucleotidase"
                     /protein_id="HCD1515198.1"
                     /translation="MTRKSIAIDMDEVLADTLGEIIDAVNFRADLGIKMEALNGQKLK
                     HVIPEHDGLITEVLREPGFFRHLKVMPHAQEVVKKLTEHYDVYIATAAMDVPTSFSDK
                     YEWLLEFFPFLDPQHFVFCGRKNIVKADYLIDDNPRQLEIFTGTPIMFTAVHNINDDR
                     FERVNGWKDVEQYFLDNIEK"
     gene            complement(11191..12108)
                     /locus_tag="NBH32_001606"
     CDS             complement(11191..12108)
                     /locus_tag="NBH32_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HCD1515199.1"
                     /translation="MENKYTHGVLFYHEHSGLKNINQGIGEVTTALSSICKHLSIQLS
                     ESEGDIIKYCQEIKTKNYAKDVDILFILGGDGTVNELINGVMAHNLQLPIGILPGGTF
                     NDFTKTLNIAPNHKQASEQMISAQVGTYDVIKVNNQYALNFVGLGLIVQNAENVQDGS
                     KDIFGKLSYIGSTVKTLLNPTQFNYQLSIDDKTYSGETTMILTANGPFIGGSRIPLTD
                     LSPQDGELNTFIFNEQSFSILNDIFKKRDSMNWNEITQGIEHIPGKKISLTTDPAMKV
                     DIDGEISLETPIDIEVIPNAIQLLTVNDL"
     gene            12199..12300
                     /locus_tag="NBH32_001607"
     CDS             12199..12300
                     /locus_tag="NBH32_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515200.1"
                     /translation="MVINEINLYRVGRENIVIAVYNEHIKFLFILIT"
     gene            complement(12378..13883)
                     /locus_tag="NBH32_001608"
     CDS             complement(12378..13883)
                     /locus_tag="NBH32_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HCD1515201.1"
                     /translation="MTQQNSHGNQIQDIPQTGFFGHPRGLGVLFFVEFWERFSYYGMR
                     ALLIFYMYFAVTDNGLGIDKTTAMSIMSVYGSLIYMTSIPGGWIADRITGTRGATLLG
                     AVFIIIGHICLSLPFALIGLFTSMFFIIIGSGLMKPNISNIVGRLYPENDRRMDAGFV
                     IFYMSVNMGALLSPIILQHFVNVKNFHGGFLIAAVGMALGLVWYVLFNRKNLGSVGMK
                     PTNPLTPAEKKKYGLIIGSVVLAIVLIIVIGALTNSLSFNLVSNTVLVLGIALPIIYF
                     TLIIRSKDVTDTERSRVKAFIPLFILGMVFWAIQEQGSNVLNIYGIEHSDMKLNLFGW
                     KTNFGEAIFQSINPLFILLLAPIISLLWQKLGTKQPSLPVKFAIGTFLAGASYILIGI
                     VGYASGSSNFSVNWVILSYIICVIGELCLSPTGNSAAVKLAPKAFNAQMMSIWYLTNA
                     SAQAINGTLVKLIEPLGQTNYFIFLGVVAIIVTTIVLAFSPLIIKAMKGIR"
     gene            complement(14221..14721)
                     /gene="queF"
                     /locus_tag="NBH32_001609"
     CDS             complement(14221..14721)
                     /gene="queF"
                     /locus_tag="NBH32_001609"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCD1515202.1"
                     /translation="MAHGRQQDELQDITLLGNQDNTYNFDYRPDVLESFDNKHQGRDY
                     FVKFNCPEFTSLCPITGQPDFATIYISYIPNVKMVESKSLKLYLFSFRNHGDFHEDCM
                     NIIMNDLIELMDPHYIEVWGKFTPRGGISIDPYTNYGRPNSKYEKMAEHRLMNHDLYP
                     EKIDNR"
     gene            complement(14741..15607)
                     /locus_tag="NBH32_001610"
     CDS             complement(14741..15607)
                     /locus_tag="NBH32_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001594836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1515203.1"
                     /translation="MNPKVKGIIAILISAIGFSFMSVFFRLAGDLPVFQKSLARNLVA
                     MFIPLFFIYKYHQPMFGKLSSQPLLLTRSTLGLIGVLLNIYAIDHMVLSDADSLMKLN
                     PFWTILLSIVFLHEKVRKYQITAMIIAILGMLLIVKPEFSSSMIPSLAGLFSGIFAAS
                     AYTCVRALSTREAPYTIVFYFSLFSVIVLIPFTAYTYEPMSQMQILYLLGAGLAAAVG
                     QIGVTLAYSFAAAKDISIFTYASIIFTAILGFILFGESPDFYATLGYVVIIGASYYMF
                     EKARRDAKIIKK"
BASE COUNT         5890 a         2283 c         2788 g         5096 t
ORIGIN      
        1 aatatgagtt gtagcccaaa atacttgtta aatcaaagtt gaaagctaca aataatgaaa
       61 tttataaact tgaatctgaa agtaattact ataattatga caatgttaac ttttaaacgc
      121 acttattaat taactacata atgttaatat ctaatttatt caagtacttt cgcaagattt
      181 attatctaaa taacggggga aagaatcatg agttcacaaa aaaagaaaat tagtcttttt
      241 gcgttcttct tattaaccgt aataacgatt accttgaaga cgtatttttc ttattatgtt
      301 gatttttctt taggtgttaa aggtttagta caaaacttaa tattattgat gaatccttat
      361 agtttagtag cactggtttt aagtgtgttc ctattcttta aaggcaaaaa agcattttgg
      421 ttcatgttca taggcggctt cttattgacg ttcctattat atgccaatgt tgtgtacttt
      481 agattcttct ctgatttttt aacgtttagt actttaaacc aagtaggtaa cgtagaatct
      541 atgggtggtg cggttagtgc atcattcaaa tggtatgact ttgtttattt cattgatacg
      601 ttagtttact tattcatttt aatatttaaa acaaaatggt tagacacaaa agcatttagt
      661 aagaaatttg ttcctgtcgt aatggcggct tcagtagcat tattcttctt aaacttagct
      721 tttgctgaaa ctgacagacc agaattatta acacgtacat ttgaccataa atatttagtg
      781 aaatatttag ggccttataa ctttacagtt tacgatggtg ttaaaactat cgaaaataat
      841 caacaaaagg cgttagcatc tgaagatgac ttaacaaaag tattaaatta tacgaagcaa
      901 cgtcaaacag agcctaaccc agaatattat ggggtggcaa agaagaaaaa tattattaag
      961 attcatttag aaagtttcca aaccttctta attaataaaa aggttaatgg taaagaagta
     1021 acaccgtttt taaacaaatt atcaagtggg aaagagcaat tcacatattt ccctaacttt
     1081 ttccatcaaa caggtcaagg taaaacatct gactctgaat ttacaatgga taacagttta
     1141 tacggcttac cgcaaggttc tgccttttca ttaaaaggag ataatacgta tcagtcatta
     1201 ccagcaattt tagatcaaaa gcaaggttac aaatctgatg ttatgcacgg tgactataaa
     1261 acattctgga acagagacca agtatataaa cactttggta tcgataaatt ctatgatgca
     1321 acatactatg acatgtctga taaaaacgtt gtaaacttag gcttgaaaga caaaatcttc
     1381 tttaaagatt ctgctaatta tcaagctaag atgaaatcac cattctattc tcatttaatt
     1441 acattgacta accactatcc attcacatta gatgaaaagg atgcaactat tgagaagtca
     1501 aacacaggtg atgcaacagt tgatggttat attcaaacag cacgttattt agacgaagca
     1561 ttagaagaat atattaatga cttgaagaaa aaaggattat atgacaattc agtgattatg
     1621 atttatggtg accactatgg tatctctgaa aaccataaca atgccatgga aaaactatta
     1681 ggtgaaaaaa tcacaccagc taaatttaca gatttaaaca gaactggttt ctggattaaa
     1741 atccctggta aatctggtgg tatcaataat gaatatgctg gtcaagtcga tgtaatgcca
     1801 acaattttac atttggctgg tatagatacg aagaattatt taatgttcgg tactgattta
     1861 ttctctaaag gtcataatca agtagttcca ttcagaaatg gtgactttat aacaaaagat
     1921 tataaatatg ttaatggtaa gatttattct aataaaaata atgaactcat aactactcaa
     1981 ccagctgatt tcgaaaagaa taaaaagcaa gttgaaaagg atctcgaaat gagtgacaac
     2041 gtgcttaatg gtgatttgtt tagattctac aaaaatccag acttcaaaaa ggtaaatcct
     2101 tcgaagtata aatatgaaac aggacctaaa gcaaactcta aaaaataata tttaaacacg
     2161 aactcggatt gataaaatat caatccgggt ttttattatg ttcttttata ttatgtttta
     2221 cattatatgt gttgataaaa aggtatatta taaagatcat tagcatagtc atcaaagcct
     2281 tatcaaattt tatttatagt tgaagcaaca atttattaaa ttatgacact taacagtatc
     2341 gatttctttt gatttcaaaa aggtgtataa tgtatagaac tgatttaagt acgtagagat
     2401 ttcaacagaa gaaggaagat gggtatggaa gcatataaaa ttgaacattt aaataaatct
     2461 tatgccgata agattatatt cgataatcta gatttatcaa tttcagaagg tgaaaaaata
     2521 ggtttagtag gcataaatgg tacagggaaa agtacattgt taaaagtaat tggtggtatt
     2581 gatgatgatt ttacagccaa tgttatgcat ccaaatcaat atcgaattcg ttattcgtct
     2641 cagaaacagg atcttaatga agatatgact gtttttgatg cagtattaag ttctgataca
     2701 acaactttac gcatcatcaa gcaatatgag caggcagtac aagcttatgc ggatgaccaa
     2761 agtgataaat tgttcaagcg aatgatggat gcgcaagatg ctatggatca acatgatgct
     2821 tgggactata acgctgaaat taaaacaatc ctctcaaaac taggtataca tgatactact
     2881 aaatacatta aagaattatc cggcggacaa caaaaacgtg ttgtacttgc taaaacatta
     2941 atagaacaac cagatttatt gttattagat gaacctacga accatttaga cttcgaatca
     3001 atcagctggt tgatcaatta tgtgaagcaa tatcctcata cagttttatt cgtaacccat
     3061 gatcgatatt ttttaaatga agtttccact agaattattg aactaaacag aggtaagtta
     3121 gcgtcatatc ctggtaacta tgaatcttat attgaaatgc gcgctgaaag agaagtaaca
     3181 cttcaaaagc aacaacaaaa gcaacgagct ttatataagg aagaacttgc ttggatgagg
     3241 gctggagcta aggctcgtac tacaaagcaa caagctagaa ttaatcgatt taatgaccta
     3301 gaaaatgaag ttaaccagca atataaagac gataaaggtg aattgaatct tgcttattca
     3361 agattaggta agcaagtgtt cgaattagaa gacttatcaa aggctattaa tgataaagta
     3421 ttatttgaac atctaacgga aattattcaa aaaggtgagc gtattggtgt tgttggtcca
     3481 aatggagctg gtaaaacaac actcttaaat attttgagtg gagaagacca acaattcgaa
     3541 ggtaaattga agactgggca gacggttaaa atagcttatt ttaagcaaac agatgagacc
     3601 ttggatagag atattcgtat gatagattat ttaagagaag aaagtgagat tgcaaaagaa
     3661 aaagatggaa cctcggtatc tattacacaa cttcttgaac gatttttatt tccaagtgca
     3721 actcatggta aaaaagttta taaattatct ggtggagagc aaaagcgttt gtatttatta
     3781 cgtctactcg tacaccagcc aaatgttctg ttgttagatg aaccgacaaa tgatttagat
     3841 accgagactt taacaatact tgaagattat attcatactt tcggtggtac agtgattacc
     3901 gtaagccatg atcgttactt cttaaataaa gttgcacagt catattggtt tattcatgat
     3961 ggtcagatgg aaaagattat cggaactttt gaagattatg aaagttataa aaaatcatta
     4021 gataaaaata aatccacatt gaagcaacaa tctaaatctt ctacaactgt acgtaagaaa
     4081 aatgggttat catataaaga aaaattagaa tatgaacaat tgatgaaacg tatagaacaa
     4141 tcggaagtaa gaatggaaga aattgatgtg ctcatgattg aggcaagtgc agattatggg
     4201 aaaattaaag aattaaacga agaaaaagaa caacttgaaa ttcaatatga tttagacatc
     4261 acaagatgga gtgagttaga agaaattaaa gaacaacaat aaggggtcaa tttatgatgc
     4321 aacaaacatt atcgcattac tttgggtatg aaacgtttcg accaggacaa gaagaaatta
     4381 ttagcaaagt attagaccat cgtaatgtgc ttggtgtctt accaactggt gggggtaagt
     4441 ctatatgcta tcaagtacca ggtttattgt taggtggtac aacgattgtt ataagtccac
     4501 taatttcatt aatgaaagat caagtggatc aattaaaggc aatggggatt caagctgctt
     4561 ttttaaacag tagtttgact caaaaagaac aacaacatat tgaaaaagaa ttaacaagtg
     4621 ggaatattca atttttatat gttgcaccag aacgatttga aaatcgatat tttttaaatt
     4681 tgcttcaacg tatcaagatt catttagtgg cgtttgatga agcgcattgt atatctaaat
     4741 ggggacatga tttcaggccg agttaccaaa atgttatttc aaaagtgttt atgttaccac
     4801 aagattttac aattattgca ttgacagcaa ctgcaacagt tgaagtacag caagatatta
     4861 gagaaaagtt aaatatcgct caaactgatc aaattaaaac gagtactaag cgtagaaact
     4921 taatttttaa agtaaatcct acttatcaac gtcaaaaatt tatattggat tatattaaaa
     4981 cacacgatga agatgcaggt attatttatt gttctacacg taagcaagtt gaagagcttc
     5041 aagaagcctt agaaagccag aacattgaaa gtgttatata tcatgcaggt ttgagcaata
     5101 aagaaagaga agaagcgcag aatgatttct tatttgatcg tgttaaagta gtcgttgcta
     5161 caaatgcttt tggtatgggt attgataaat ccaatgtacg ctttgttatt cattataata
     5221 tgcctggaga tttagaatct tattatcaag aagcgggtcg tgcaggtcgt gacgggttga
     5281 aaagtgaatg tattttgtta tttagcgaac gcgatatcaa tttacacgag tattttataa
     5341 cagtctctca agctgatgat gactataaag ataaaatggg tgaaaagtta actaaaatga
     5401 ttcaatatac aaaaacaaaa aaatgtttag aagcaacaat tgtccattat tttgaaccga
     5461 atgaaaaatt agaagaatgt gaacaatgta gtaattgtgt tcaacaagat aaatcatata
     5521 atatgacaca agaagctaag atgattatta gttgcatcgc tcgtatgaaa caacaagaga
     5581 gttatagtgt tatcatccaa gtattaagag gagagtcaac agattatatt aagtataaag
     5641 gttatgacca aatttcaacc catggtttaa tgaaaggtta cacaacatca gaattaagtc
     5701 acttaataga tgaattaaga ttcaaagggt tcttaaatga aaatgatgaa atattaatgt
     5761 gtgatacttc aattaaaaaa ttactcagta atgaagtaga agtattcaca acaccattta
     5821 agcaaaaagc gactgaaaaa gtatttataa atacggttga aggggttgac cgagcattat
     5881 tcagtcagtt ggtagaagtt cgtaaaaagt taagtgacaa attaacgata gcacctgtaa
     5941 gtatattttc tgattacacg ctagaggaat ttgctaaacg taagcctgct tcgaaacaag
     6001 atatgattaa tattgatggc gtaggtagtt acaaattaaa acattattgt ccagcatttt
     6061 tagaaacgat tcaaaattat aaagccaaag tatagtgaag gcacctagta aacattcctt
     6121 gttttactgg gtgttttttg aattcaaagt aaaataacac tatttcttaa ctggagatta
     6181 cctattattc attattttgt taaaaaagtt caaggataaa tcatttaaat ttgaaaattg
     6241 taataaacgt ggttaaatag ggaatacata catataatct cggttttatg ctatgagaaa
     6301 aaaagaggtg gcaaagtgat taagtttaaa aatgtaacta agcgttatgg caaacatgtt
     6361 gctgtcgata acattagttt caatattaat gagggtgaat tttttgtgct cattggacct
     6421 tcaggttgtg gaaaaactac gacattaaaa atgattaatc gactcattca cttaagtgaa
     6481 ggttatattt actttaaaga taaaccaata agtgattatc cagtatacga aatgcgttgg
     6541 gatattggat acgtattgca gcagattgca ttattcccac atatgacaat caaagaaaat
     6601 attgcacaag tgccacaaat gaaaaagtgg aaagaaaaag atatagataa aagagtagat
     6661 gaattacttg aaatggttgg attagaacct gaaaaatata aaaacagaaa acctgatgaa
     6721 ttgtcagggg ggcaacgaca acgtgtagga gttatacgtg cgttagcagc tgatccacca
     6781 gttattttaa tggatgaacc gtttagtgca ttagacccaa tcagccgaga aaaacttcaa
     6841 gatgatttaa ttgaattaca aactaaaatt aagaagacaa tcatatttgt tacacatgat
     6901 attcaagagg cgatgaaact tggtgataag atttgtcttt tgaatgaagg gcatattgaa
     6961 caaattgaca caccagaagg atttaaaaat aatcctcaaa gtgaatttgt taaacaattt
     7021 atgggtagtc atttagaaga tgatgcgcca tgtgttgaag agaacgcaat tatccgtgac
     7081 ttggatatta tgaaaccaat cgatgaggtt acatctatga gcgcttatcc aattgtttat
     7141 gacaatcaac caattgaagt attgtatcaa cttttatcag agagcgagcg tgtcattgtc
     7201 atgcaagaag atagcgtagg tcaatatgtt attgatagga aagatatctt caaatatttg
     7261 tcccagaaaa aggaggtagc tcaacatgac taactttttc gacatattga gtgaacgtaa
     7321 gggacaactc ttttcgacaa tgatagaaca tattcaaata tcatttatcg cattattgat
     7381 tgcaactgct attgcggtac cattaggtat tttattaacg aagactaaaa cgatatctga
     7441 aatcgtaatg aatattgcgg caattcttca aaccatacca tcgttggcat tattaggttt
     7501 aatgattcct ttatttggta tcggtcgtgt gccagcaatt atcgcacttg tagtgtatgc
     7561 gttgttacca attttaagga atacgtatac tggaattaaa gaagttgatc catcactcat
     7621 tgaagcggct aaaggtatag gtatgaaacc atttagacgt ttaactaaag tcgaacttcc
     7681 gatagcaatg cctgttataa tggctggtgt aagaacggct atggtattaa ttataggtac
     7741 agcaacacta gcagcattaa ttggtgcagg cggactagga gatttaattt tattaggtat
     7801 agaccgtaac aatgcatcat tgatattatt aggtgcaatt ccagcagcct tattggcaat
     7861 tatatttgat ttaattttaa gatttatggc taaattatct tataaaaagt tattgatgac
     7921 gttaggtgtt atagtgatga ttattatact ggctatcgct attcctatgt ttgcacaaaa
     7981 aggtgataaa attacgttag ctggaaagct tggctccgag ccttcgatta ttacaaatat
     8041 gtataaaatt ttaatagaag aagagaccaa aaatactgta gaagtgaaag atggtatggg
     8101 gaaaacagca tttttattta atgctttaaa atctgatgat atagatgggt atttagagtt
     8161 tactggaaca gttttaggtg aattaacaaa agaaccattg aagtcaaaag aagagaaaaa
     8221 agtttatgaa caagctaagc aaagtcttga aaagaaatat caaatgacta tgttaaaacc
     8281 aatgaagtat aacaatacgt atgctttagc tgtaaaacgt gattttgcta aacaacataa
     8341 tatacgtaca attggtgatt taaataaggt taaagatcaa cttaaaccag gatttacatt
     8401 ggaatttaat gatcgtccag atggttacaa agctgttcaa aaggcttata atttaaattt
     8461 agataacata cgtacaatgg aacctaagtt gagatatcaa gcgatcaata aaggtaatat
     8521 taatttaata gatgcatatt caactgacgc tgaattaaaa caatatgata tggttgtgtt
     8581 aaaagatgat aagcacgtat ttccaccata tcaaggagca ccattattta aagaaagctt
     8641 tttaaagaaa catccagaaa ttaagaaacc gttaaacaaa ttagaaaaca aaatatctga
     8701 tgaagatatg caagtgatga actataaagt aacagttaaa aatgaagacc catatacagt
     8761 tgcgaaagat tatttaaaag caaaagggtt aatcaaataa cgaccaacgc cacataagat
     8821 gaggggcacg ccattatatc ttatgtggcg ttgctatatt tacatctatg tttatgtaca
     8881 atttaaacat acaatattaa taattgaatt tgttatgcta attactattt acattagtaa
     8941 tgtatgaaaa tagtagcatt gcattttaga aacatagagc gatataatga atataagttt
     9001 tttaaaattt cagttaattc taaggaggtt gtttttatta tgaaagaaca acttaatcaa
     9061 ctatcagcat atcagcctgg tttatctcca agggcattga aagaaaagta tggcattgaa
     9121 ggagatttat ataaacttgc atcaaatgaa aatttgtatg gaccatcgcc aaaagttaaa
     9181 gaagcgatat cagcacactt agatgagtta tattattatc ctgaaacagg atcaccgaca
     9241 ttaaaagcgg cgattagtaa acatttaaat gtagatcaat cacgcatttt atttggtgcg
     9301 ggattagatg aagttatatt aatgatttct agagctgtat taacgccagg ggatactatt
     9361 gttacaagtg aagcgacatt cggtcaatat tatcacaatg cgattgttga atcagctaat
     9421 gtgatacaag tacctttaaa agatggtggc ttcgatttag aaggtatttt aaaagaagtt
     9481 aatgaagata cgtcattggt atggttatgt aatccaaata atcctacagg tacatatttt
     9541 aatcatgaga gcttagattc gtttttatct caagtacctc cacatgtacc agtaattata
     9601 gatgaagctt attttgaatt tgtgacagca gaggactacc cggatacact tgctttgcaa
     9661 caaaaatatg acaatgcttt cttattacgt acattttcaa aggcgtatgg attagcgggt
     9721 ttacgtgtag gatatgtggt agcaagtgaa catgcgattg aaaaatggaa catcattaga
     9781 ccaccattta atgtgacacg tatatctgaa tacgcagcag ttgcagcact tgaagatcaa
     9841 caatatttaa aagaggtaac acataaaaat agtgttgaac gcgaaagatt ttatcaatta
     9901 cctcaaagtg agcatttctt accaagtcaa acgaatttta tatttgtaaa aacaaagcgg
     9961 gtaaatgaac tttatgaagc gcttttaaat gtagggtgta ttacgcgacc atttccaact
    10021 ggtgttagaa ttacaattgg ttttaaagaa caaaatgata aaatgctaga agttttatca
    10081 aactttaaat actaatagaa aagggcacca catggtatga atatgtgcca aaatgcagtg
    10141 tgaattatga ggtgagagca tttattgtaa aaagcaacaa tagatgatat tgttttgtat
    10201 caattgcttt tttgctatac tgaattaata ctgatatttt gaggagaaga ttaaaatgac
    10261 ccgtaaatca atcgcgattg atatggatga agtattggca gatacattag gagaaatcat
    10321 tgatgctgtc aattttagag cggatttagg tattaaaatg gaagctttga atggtcaaaa
    10381 acttaaacat gttattcctg aacatgatgg attaattaca gaagtattga gagaaccagg
    10441 cttcttcaga catcttaaag tgatgccgca tgcacaagaa gttgtaaaaa aattaactga
    10501 acattatgat gtatatattg ctacagcagc aatggatgta ccaacatcat ttagtgataa
    10561 atatgaatgg ttactagagt tctttccatt cttagatcca cagcattttg ttttttgtgg
    10621 tagaaaaaac attgttaaag ctgattattt aatagatgac aatcctagac agcttgaaat
    10681 ttttactggt acaccgatta tgtttacagc agtgcataat attaatgatg atcgatttga
    10741 acgcgtaaat ggttggaaag atgtagaaca gtatttttta gataatattg agaaataaaa
    10801 atatatcact tgaagcattt tatgtagcaa agatgatgga tatactatta tgaagttggg
    10861 actggacgac gaaataaatt ttgcgaaaat atcatttccg tcccgctctc aataaatgtt
    10921 agtctatctc attattataa atcggatgaa tgtgtaaatc tatggcagat tacacgtcat
    10981 ccgatttttt ataggatttg aaaaaggcgc ataaaccact atgatttaaa atacaacatc
    11041 aatcatttta gtggcatgcg ccaaaattat atgtctgttt ttgaaacagg gtaatagctt
    11101 aaagctaata aaaacgaata taaggtgcgt tgaatcttat gattacactc taaacctaat
    11161 ataatatccc gttaagatca ttccggatgc ttacaaatca ttgacagtaa gtaactgaat
    11221 ggcatttggt ataacctcaa tatcaatagg tgtttctaat gaaatttcgc catcaatatc
    11281 aactttcatt gctggatctg ttgtaagtga aatcttttta ccaggtatat gctcaatacc
    11341 ttgagtaatt tcattccaat tcatgctatc acgcttttta aaaatatcat ttaaaatact
    11401 gaaactttgt tcattaaaaa tgaaagtgtt cagttcacca tcttgaggag acaaatcagt
    11461 caatggtata cgactaccac caatgaatgg accatttgct gtaagtatca tggtcgtttc
    11521 gccagaatat gtcttatcat caattgataa ttgataatta aattgtgttg gatttagcag
    11581 tgttttaaca gttgatccaa tataacttaa tttaccaaat atatcttttg acccatcttg
    11641 tacgttttca gcgttttgaa caattagacc taagccaaca aagttgagtg catattgatt
    11701 atttactttt attacatcgt atgtaccaac ttgtgcagaa atcatttgtt cactagcttg
    11761 tttatgatta ggtgcaatat ttagcgtttt tgtaaaatca ttaaaagtac cgcctggtaa
    11821 aatgccgata gggagttgaa gattatgtgc cataacacca tttataagtt cgttaaccgt
    11881 gccatcaccg ccaagaataa ataatatatc tacatctttt gcatagtttt tagttttgat
    11941 ttcttggcaa tatttaataa tgtcaccttc gctttcactc aattgaatag aaagatgctt
    12001 acaaattgaa cttaatgctg ttgtaacttc cccaatacct tgattaatat tttttaatcc
    12061 actgtgttca tggtaaaaga ggacaccatg tgtatatttg ttttccatag tttagcctac
    12121 tttctaaaaa ttggttcatt aaatatatat acccactttt aattgctaat accaaaaata
    12181 tgtttttaaa tagagaaaat ggtaataaat gaaattaatc tctatagagt gggacgagaa
    12241 aatatagtta tagctgtcta taatgagcat attaagtttt tatttatact gataacttga
    12301 atttaattaa tggaaaccta taaaaaaaca gtaagccatt taaatgactt actgtttttt
    12361 aatttaggtc aacaatatta acgaatacct ttcattgctt taataattaa aggtgagaat
    12421 gctaatacaa ttgttgtaac aataattgca acaacaccta agaaaataaa gtaatttgtt
    12481 tgacctagtg gttctattaa cttaactaat gtaccattga ttgcttgtgc agaagcgtta
    12541 gttaagtacc aaatactcat catttgggca ttaaatgctt taggtgctaa cttaacagca
    12601 gcactattac ccgttggtga taagcatagc tcaccgataa cacaaataat gtacgataaa
    12661 ataacccagt taactgagaa gtttgatgaa cctgatgcat aacctacaat accaattagt
    12721 atgtatgacg cacctgctaa gaacgtacca attgcaaatt ttactggcag gctaggttgt
    12781 ttagttccaa gcttttgcca taaaagtgaa ataattggag ctaataataa aataaataat
    12841 gggttaattg attggaagat cgcttcacca aagtttgttt tccaaccaaa taagtttaat
    12901 ttcatatctg aatgttcaat tccataaatg tttaatacat tagacccttg ttcttgaata
    12961 gcccagaaca ccattccaag aataaataat ggaataaatg ctttaacacg agaacgttca
    13021 gtatcagtga catctttact tctaataatt aaagtgaagt aaatgattgg taatgcaata
    13081 cctaatacta aaacagtatt actaactaag ttaaatgata atgagttagt taatgcacca
    13141 ataacgataa ttaatacaat tgctaagaca acacttccga taataagacc atactttttc
    13201 ttttcagctg gtgtcaatgg gttagtaggt ttcataccaa cgctacctaa gtttttgcgg
    13261 ttgaaaagta cataccatac taaacctaat gccataccaa ctgctgcaat caagaatccg
    13321 ccgtggaagt ttttaacatt aacaaagtgt tgcaaaataa taggtgataa taatgcaccc
    13381 atattaactg acatatagaa aataacaaaa cctgcatcca tacgtctatc attttcagga
    13441 tataaacggc caacgatatt tgaaatattt ggcttcatta aacctgaacc aataatgatg
    13501 aagaacattg atgtgaataa gccgattaat gcaaatggta agcttaaaca aatatgtccg
    13561 ataataataa agactgcacc taataaagta gcgcctctag tacctgtaat tctgtcagca
    13621 atccatccgc ctggtattga tgtcatatag attaatgaac cataaactga cataattgac
    13681 atagctgttg ttttatcaat tccaaggcca ttatctgtta cggcaaagta catgtagaaa
    13741 atgagtaggg cacgcatgcc ataataacta aacctttccc agaactctac aaagaagagt
    13801 acacctagtc ctcgaggatg cccgaaaaat cctgtttgag gtatgtcttg aatttgattt
    13861 ccatgggagt tttgttgtgt catttataca tcccatcctt tcttccccta atacaatagt
    13921 ttaagtaaat ttgattgtac cttatcaact aaatattcca ataaatcaca aaaaaataaa
    13981 aatattgaga atattctgtt attaagcgcg cacaattaat tagagataat ataaaaatat
    14041 acttattgac gttaaagtgc aataggtttc tataaaaatg ttgtttaatt agtgtattaa
    14101 tattcttttg gtgtactttg ggatttaatg attgaagtta atttacaata tatattaaaa
    14161 aagcacatca acaagttggt agtaaccaac atactgatgt gctttgaaaa gggaaacaaa
    14221 ttaacggtta tctatttttt ctggatataa atcatgattc attagacgat gctcagccat
    14281 tttttcatat ttagaatttg gacgtccata gtttgtataa gggtcaatag aaattccacc
    14341 acgtggtgtg aacttgcccc aaacttcaat ataatgtgga tccataagct ctatcaagtc
    14401 attcataata atattcatac aatcttcgtg gaaatcaccg tgatttctga aactaaataa
    14461 gtaaagtttc aacgattttg attcaaccat tttaacattt ggaatataag aaatgtagat
    14521 agttgcaaaa tctggttgcc cagtaatcgg acataatgat gtgaattctg gacagttgaa
    14581 ttttacgaaa tagtcgcgac cttgatgttt attatcgaat gattctaaaa catcaggacg
    14641 atagtcaaaa ttgtaagtat tgtcttgatt tcctaataaa gttatatctt gtaattcatc
    14701 ttgttgacgg ccatgtgcca tataaagcgc tcctttaaat ttattttttt attattttgg
    14761 cgtctcggcg tgctttttca aacatgtaat aacttgcacc gataataacg acgtaaccta
    14821 atgttgcata gaaatctgga gattctccaa atagaataaa tccaagtatt gctgtaaaaa
    14881 ttatagatgc atacgtgaaa atagaaatat ctttagcagc agcaaaacta tatgccaatg
    14941 taacaccaat ttgacccaca gcggcagcta agccagcccc taatagataa agtatttgca
    15001 tctgactcat tggttcatat gtatatgcag taaatggtat taatacgatg actgaaaata
    15061 atgagaagta aaatacaatg gtgtatggtg cttctctagt actaagtgcg cgaacacatg
    15121 tatatgccga tgctgcaaaa atacctgaaa atagtccagc tagtgatgga atcatagatg
    15181 atgaaaattc aggtttcact attaaaagca tacctaaaat agcaattatc attgctgtta
    15241 tttgatactt ccttaccttt tcatgtaaga aaacaatact taataaaatc gtccagaaag
    15301 gattgagttt cattaatgaa tcggcatcac taagtaccat atggtcaatg gcgtaaatat
    15361 ttaataatac acctataagt ccaagtgtcg aacgtgttaa taataatggt tggcttgaga
    15421 gtttaccaaa cattggttga tgatatttat atataaaaaa taatggaata aacattgcta
    15481 ctaagtttcg tgctaatgat ttttgaaaaa caggaagatc acctgcaagt ctgaaaaaca
    15541 ctgacataaa actgaaacca atagccgaaa ttaaaatggc aatgatacct tttactttag
    15601 gattcaattt tatcgcctct tttatataaa attaacgtat ttatattagc ataaaacaac
    15661 atgttgtgca taaatagttg aaatttacta taaaaagcct ataatagact gtaccgaaca
    15721 aacgttctgt gtttatttgt cggaataata gggcattaca cttttatgaa tgtttgtgtt
    15781 attacataaa acaaatatca attcagtatc aagctaataa gctttttctt aatttctgtt
    15841 gatacaattg agattgacgc agattttaaa aaatcaagtg atatctactg aaaaaatttt
    15901 tttaatttgt tcaagttttt ctaattaagt attggtgcct agttggaacg ttttacgaac
    15961 attcgattag aaaatggcac tttaaatcat agtgtgtctt atgtataatg aaacacataa
    16021 tatagtgttg gtgaaacaaa aaagacacaa tatcttg
//
LOCUS       DAHHOJ010000059        15888 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.059, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000059 DAHHOJ010000000
VERSION     DAHHOJ010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15888
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.059"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(304..1005)
                     /gene="lrgB"
                     /locus_tag="NBH32_001611"
     CDS             complement(304..1005)
                     /gene="lrgB"
                     /locus_tag="NBH32_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like protein LrgB"
                     /protein_id="HCD1515204.1"
                     /translation="MINHLALNTPYFGILLSVIPFFLATILFEKTNRFFLFAPLFVSM
                     VFGVAFLYLTGIPYKTYKIGGDIIYFFLEPATICFAIPLYKKREVLVKHWHRIIGGIG
                     IGTVVALLIILTFAKLAQFANDVILSMLPQAATTAIALPVSAGIGGIKELTSLAVILN
                     GVIIYALGNKFLKLFRITNPIARGLALGTSGHTLGVAPAKELGPVEESMASIALVLVG
                     VVVVAVVPVFVAIFF"
     gene            complement(998..1441)
                     /gene="lrgA"
                     /locus_tag="NBH32_001612"
     CDS             complement(998..1441)
                     /gene="lrgA"
                     /locus_tag="NBH32_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiholin-like murein hydrolase modulator LrgA"
                     /protein_id="HCD1515205.1"
                     /translation="MVVKQQKDASKPAHFFHQVIVIALVLFVSKIIESFMPIPMPASV
                     IGLVLLFVLLCTGAVKLGEVEKVGTTLTNNIGLLFVPAGISVVNSLGVISQAPFLIIG
                     LIIVSTILLLICTGYVTQIIMKVTSRSKGDKVTKKIKIEEAQAHD"
     gene            complement(1554..2294)
                     /locus_tag="NBH32_001613"
     CDS             complement(1554..2294)
                     /locus_tag="NBH32_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor LytR"
                     /protein_id="HCD1515206.1"
                     /translation="MKALIIDDEPLARNELTYLLNEIGGFEEINEAENVKETLEALLI
                     NQYDIIFLDVNLMDENGIELGAKIQKMKEPPAIIFATAHDQYAVQAFELNATDYILKP
                     FGQKRIEQAVNKVRATKAKDDNSASAIANDMSANFDQSLPVEIDDKIHMLKQQNIIGI
                     GTHNGITTIHTTNHKYETTEPLNRYEKRLNPAYFIRIHRSYIINTKHIKEVQQWFNYT
                     YMVILTNGVKMQVGRSFMKDFKASIGLL"
     gene            complement(2297..4051)
                     /locus_tag="NBH32_001614"
     CDS             complement(2297..4051)
                     /locus_tag="NBH32_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1515207.1"
                     /translation="MLSLTMLLLERVGLIIILAYVLMNIPYFKNLMNRRRTWKARWQL
                     CIIFSLFALMSNLTGIVIDHQHSLSGSVYFRLDDDVSLANTRVLTIGVAGLVGGPFVG
                     LFVGVISGIFRVYMGGADAQVYLISSIFIGIIAGYFGLQAQRRKRYPSIAKSAMIGIV
                     MEMIQMLSILTFSHDKAYAVDLISLIALPMIIVNSVGTAIFMSIIISTLKQEEQMKAV
                     QTHDVLQLMNQTLPYFKEGLNRESAQQIAMIIKNLMKVSAVAITSKNEILSHVGAGSD
                     HHIPTNEILTSLSKDVLKSGKLKEVHTKEEIGCSHPNCPLRAAIVIPLEMHGSIVGTL
                     KMYFTNPNDLTFVERQLAEGLANIFSSQIELGEAETQSKLLKDAEIKSLQAQVSPHFF
                     FNSINTISALVRINSEKARELLLELSYFFRANLQGSKQHTITLDKELSQVRAYLSLEQ
                     ARYPGRFNININVEDKYRDVLVPPFLIQILVENAIKHAFTNRKQGNDIDVSVIKETAT
                     HVRIIVQDNGQGISKDKMHLLGETSVESESGTGSALENLNLRLKGLFGKSAALQFEST
                     SSGTTFWCVLPYERQEEE"
     gene            complement(4297..4971)
                     /gene="scdA"
                     /locus_tag="NBH32_001615"
     CDS             complement(4297..4971)
                     /gene="scdA"
                     /locus_tag="NBH32_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair di-iron protein ScdA"
                     /protein_id="HCD1515208.1"
                     /translation="MINKNDIVADVVTDYPKAADIFRSVGIDFCCGGQVSIEAAVLEK
                     KNVDLNELLQRLNEVEQTNTPGSLNPKFLNVSSLIQYIQAAYHEPLREEFKNLTPYVT
                     KLSKVHGPNHPYLVELKATYDTFKNGMLEHMQKEDDVDFPKLIKYEQGEVVDDINTVI
                     DDLVSDHIATGQLLVKMSDLTSSYEPPIEACGTWRLVYQRLKALEVLTHEHVHLENHV
                     LFKKVS"
     gene            complement(5115..6836)
                     /gene="tarS"
                     /locus_tag="NBH32_001616"
     CDS             complement(5115..6836)
                     /gene="tarS"
                     /locus_tag="NBH32_001616"
                     /EC_number="2.4.1.355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000975351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(ribitol-phosphate)
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCD1515209.1"
                     /translation="MMKFSVIVPTYNSEKYITELLNSLAKQDFPKTEFEVVVVDDCST
                     DQTLQIVEKYRNKLNLKVSQLETNSGGPGKPRNVALKQAEGEFVLFVDSDDYINKETL
                     KDAAAFIDEHHSDVLLIKMKGVNGRGVPQSMFKETAPEVTLLNSRIIYTLSPTKIYRT
                     ALLKDNDIYFPEELKSAEDQLFTMKAYLNANRISVLSDKAYYYATKREGEHMSSAYVS
                     PEDFYEVMRLIAVEILNADLEEAHKDQILAEFLNRHFSFSRTNGFSLKVKLEDQPQWI
                     NALGDFIQAVPERVDALVMSKLRPLLHYARAKDIDNYRTVEESYRQGQYYRFDIVDGK
                     LNIQFNEGEPYFEGIDIAKPKVKMTAFKFDNHKIVTELTLNEFMIGEGHYDVRLKLHS
                     RNKKHTMYVPLTVNANKQYRFNIMLEDIKADLPKEKIWDVFLEVQIGTEVFEVRVGNQ
                     RNKYAYTAETSALIHLNNDFYRLTPYFTKDFNNISLYFTAITLTDSISMKLKGKNKII
                     LTGLDRGYVFEEGMASVVLKDDMVMGMLSQTSENEVEILLSKDIKKRDFKNIVKLNTA
                     HMTYSLK"
     gene            complement(6869..8557)
                     /locus_tag="NBH32_001617"
     CDS             complement(6869..8557)
                     /locus_tag="NBH32_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1515210.1"
                     /translation="MVKSKIYIDKIYWERVQLFVEGHSENLDLEDSNFVLRNLTETRT
                     MKANDVKIDGNQFVCRFNVAILDNGYYLPEDKYLLVNEQELDYIAQLNPDVINDVYQN
                     LKPEQEEEYNELETQNGKINFLLQTYLKEFRKGGISKKTVYTVTPEISSDVNEFVLDV
                     VVTTPEVKSIYIVRKYKELRKYFRKQSFNTRQFIFKAIFNTTKFFHLKKGNTVLFTSD
                     SRPTMSGNFEYIYNEMLRQNLDKKYDIHTVFKANITDRRGIIDKFRLPYLLGKADYIF
                     VDDFHPLIYTVRFRRSQEVIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKAYV
                     SSETDIPFYAEAFGIKEKNVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKVIL
                     FAPTFRGSGHGTAHYPFFKIDFERLARYCEKNNAVVLFKMHPFVKNRLNIADKHKQYF
                     VDVSDFREVNDILFITDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEPYE
                     SFVPGKIVQSFDALMDALDNEDYEGEKVIPFLDKHFKYQDGRSSERLVRNLFGS"
     gene            complement(8579..9604)
                     /locus_tag="NBH32_001618"
     CDS             complement(8579..9604)
                     /locus_tag="NBH32_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD1515211.1"
                     /translation="MINQVYQLVAPRQFEVTYNNVDIYSDYVIVRPLYMSICAADQRY
                     YTGSRDENVLSQKLPMSLIHEGVGEVVFDSKGVFNKGTKVVMVPNTPTEKDDVIAENY
                     LKSSYFRSSGHDGFMQDFVLLNHDRAVPLPDDIDLSIISYTELVTVSLHAIRRFEKKS
                     ISNKNTFGIWGDGNLGYITAILLRKLYPESKIYVFGKTDYKLSHFSFVDDVFFIDKIP
                     EGLTFDHAFECVGGRGSQSAINQMIDYISPEGSIALLGVSEFPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFQDVVDLYIQYPDIVDKLALLKGQEFEIATINDLTEAFEADLSTSWGK
                     TVLKWIM"
     gene            complement(9597..10313)
                     /locus_tag="NBH32_001619"
     CDS             complement(9597..10313)
                     /locus_tag="NBH32_001619"
                     /EC_number="2.7.7.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribitol-5-phosphate cytidylyltransferase"
                     /protein_id="HCD1515212.1"
                     /translation="MKYAGILAGGIGSRMGNVPLPKQFLDLDNKPILIHTLEKFILIN
                     DFEKIIIATPQQWMTHTKDTLRKFKISDERIEVIQGGSDRNDTIMNIVKHIESTNGIN
                     DDDVIVTHDAVRPFLTHRIIKENIQAALEYGAVDTVIDAIDTIVTSKDNQTIDAIPVR
                     NEMYQGQTPQSFNINLLKESYAQLSDEQKSILSDACKIIVETNKPVRLVKGELYNIKV
                     TTPYDLKVANAIIRGGIADD"
     gene            complement(10589..11758)
                     /locus_tag="NBH32_001620"
     CDS             complement(10589..11758)
                     /locus_tag="NBH32_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1515213.1"
                     /translation="MIKTTIKKLIEHSIYTTFKLLSKLPNKNLIYFESFHGKQYSDNP
                     KALYEYLTEHSDAQLIWGVKKGYEHIFQQHNVPYVKKFSMKWFLAMPRAKAWMINTRT
                     PDWLYKSPRTTYLQTWHGTPLKKIGLDISNVKMLGTNTQNYQDGFTKESQRWDYLVSP
                     NPYSTSIFQHAFHVSRDKILETGYPRNDKLSHKRNDTEYINGIKTRLNIPLDKKVIMY
                     APTWRDDEAIREGSYQFNVNFDIEALRQALDDDYVILLRMHYLVVTRIDEHDDFVKDV
                     SDYEDISDLYLISDALVTDYSSVMFDFGVLKRPQIFYAYDLDKYGDELRGFYMDYKKE
                     LPGPIVENQTALIDALKQIDETANEYIEARTVFYQKFCSLEDGKASQRICQTIFK"
     gene            complement(12187..13881)
                     /locus_tag="NBH32_001621"
     CDS             complement(12187..13881)
                     /locus_tag="NBH32_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000167965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HCD1515214.1"
                     /translation="MTKTKQAIHIDNIYWERVQLYIEGHSEGVDLTSGQFVLRNLTET
                     KTLEANEMKIDGNTFICRFNVAILDDGYYLPMDKYLFVYHDQLEYIGQLNPNIIDQAY
                     AALNEEQIEEYNELTTQNGKANYVLANEAKVFRKGGVSQHTVYTITPEIASDVNEFVF
                     DIEITLPQEKSGVIATSAHWLHKQGHKASFESRSFLFKAIFNITKLLHIKRSKTILFT
                     SDSRPNLSGNFKYVYDELLRQKVDFDYDIKTVFKANITDRRKWRDKFRLPYLLGKADY
                     IFVDDFHPLIYTVRFRPSQEIIQVWHAVGAFKTVGFSRTGKKGGPFIDSLNHRSYTKA
                     YVSSETDIPFYAEAFGIREENVVPTGVPRTDVLFDEAYATQIKQEMEDELPIIKGKKV
                     ILFAPTFRGNGHGTAHYPFFKIDFERLARYCEKHNAVVLFKMHPFVKNRLNISREHRQ
                     YFIDVSDHREVNDILFVTDLLISDYSSLIYEYAVFKKPMIFYAFDLEDYITTRDFYEP
                     YESFVPGKIVQSFDALMDALDNEDYEVEKVVPFLDKHFKYQDGRSSERLVKDLFRR"
     gene            complement(13903..14928)
                     /locus_tag="NBH32_001622"
     CDS             complement(13903..14928)
                     /locus_tag="NBH32_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HCD1515215.1"
                     /translation="MINQVYQLVAPRQFDVTYNNVDIYGNHVIVRPLYLSICAADQRY
                     YTGRRDENVLRQKLPMSLVHEAVGEVVFDSTGVFEKGTKVVMVPNTPTEQHHIIAENY
                     LASSYFRSSGYDGFMQDYVVMAHDRIVPLPNDIDLSTISYTELVSVSYHAIQRFERKS
                     IPLKTSFGIWGDGNLGYITAILLRKLYPEAKIYVFGKTDYKLSHFSFVDDIFTVNQIP
                     DDLKIDHAFECVGGKGSQVALQQIVEHISPEGSIALLGVSELPVEVNTRLVLEKGLTL
                     IGSSRSGSKDFEQVVDLYHKYPDIVEKLALLKGHEINVCTMQDIVQAFEMDLSTSWGK
                     TVLKWTI"
     gene            complement(14921..15637)
                     /locus_tag="NBH32_001623"
     CDS             complement(14921..15637)
                     /locus_tag="NBH32_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCD1515216.1"
                     /translation="MIYAGILAGGIGSRMGNVPLPKQFLDIDNKPILIHTIEKFILVS
                     EFNEIIIATPAQWISHTQDILKKYNITDQRVKVVAGGTDRNETIMNIIDHIRNINGIN
                     NDDVIVTHDAVRPFLTQRIIKENIEVAAKYGAVDTVIEAIDTIVMSKDKQNIHSIPVR
                     NEMYQGQTPQSFNIKLLQDSYRALSSEQKEILSDACKIIVESGHPVKLVRGELYNIKV
                     TTPYDLKVANAIIQGDIADD"
BASE COUNT         5006 a         2953 c         2262 g         5667 t
ORIGIN      
        1 gcatacgccg tttttttata aagtgttgat gacattaagt aatttcaaga atttgtcgaa
       61 aaatatttaa ttgaaatttc acaaatgata taacttacat tttaattctt gtttaaatta
      121 attcgttcta ccatttttca aaagtggcaa tacataaaac gaacgcctaa ataccataat
      181 tcctagtaaa aagtaataca tacataaaaa cgtctatatc ccagttataa accggagtat
      241 agacgttttt ttacgataat aacaatggct caaattgcta ttatcttgct taggtttttc
      301 gttttagaag aatattgcta caaagacagg cacaactgct acaacaacta caccaactaa
      361 cactaaagct atacttgcca ttgattcttc tacaggtcct aattctttgg ctggtgctac
      421 acctaatgtg tgaccacttg ttccaagtgc taatcctcgg gcaatagggt tagtaattcg
      481 gaaaagcttc aagaatttat tacctagggc ataaataatg acaccattta aaataactgc
      541 taatgatgtt aattctttta taccaccgat accagctgat actggtaacg caatcgctgt
      601 agttgctgct tgaggtaaca ttgataaaat aacatcattg gcaaattgtg ctaacttcgc
      661 aaaagttaaa ataattaata acgctacaac tgtaccgata ccaatacctc cgatgatacg
      721 atgccaatgt ttaacaagca cttcacgctt tttatataac ggaatcgcaa aacagattgt
      781 tgccggttct aagaagaagt aaataatatc tccacctatt ttgtaagtct tatacggaat
      841 gcctgttaaa tagaggaagg ccacaccaaa taccatactg acaaatagcg gtgcgaataa
      901 gaagaaacga ttagtttttt caaataatat ggtcgctaag aaaaatggta taacggataa
      961 cagtattccg aagtaaggtg tgtttagtgc taagtggtta atcatgagct tgtgcctcct
     1021 ctattttgat cttttttgtg actttgtcac ctttagatct cgaagtaact ttcataataa
     1081 tttgtgtgac atagccagta caaataagta atagtattgt tgagacgatt attagtccaa
     1141 tgattaaaaa tggtgcttgg ctaatgacac ctaaagagtt aacaactgag ataccggctg
     1201 gtacgaagag taagccaatg ttatttgtta gtgtcgttcc tactttttcg acttcgccta
     1261 acttaacagc accagtacat aataatacaa ataataatac taaaccgatt actgatgcag
     1321 gcataggaat tggcataaat gattcaatta ttttcgatac aaagagtact aaagcaatta
     1381 caatgacttg gtgaaaaaag tgtgctggtt ttgatgcgtc tttttgttgt ttcacgacca
     1441 ttgcctccta cgtttgattt aactaaagta tagatggctc acttcgattt gcgtgatttt
     1501 tagtccgaaa tacaaaatat cataggtaaa atgcataaaa aaaaggatta ctgttaaagt
     1561 aatcctatcg acgctttaaa atctttcata aatgaacgtc caacttgcat cttgacacca
     1621 tttgtcaata ttaccatata agtgtagtta aaccattgtt gcacttcttt aatgtgtttc
     1681 gtgttaataa tatatgaacg atgaatacgt atgaaataag cgggattcaa tcgtttttca
     1741 taacgattca atggctctgt tgtttcgtat ttatgatttg ttgtatgtat ggttgtaata
     1801 ccattatgtg tgccaatccc aataatattt tgttgcttta acatgtgaat tttatcgtca
     1861 atttcaacag gtaaactttg atcaaaattc gccgacatat cattcgcaat tgcacttgcg
     1921 ctattatcat ctttggcttt agtcgcacga actttattga ctgcttgttc aatacgtttt
     1981 tgaccaaacg gtttcaaaat ataatctgtc gcatttaatt caaatgcctg tactgcgtat
     2041 tggtcatgtg cagttgcaaa aataatcgca ggtggctctt tcatcttttg aattttagct
     2101 cctaattcga taccattttc atccattaaa ttgacatcta aaaatataat gtcatattga
     2161 tttatcagta gtgcttccaa tgtttctttt acattttctg cctcattaat ttcttcaaaa
     2221 ccaccaattt catttaataa atatgttaat tcattacgtg ctaatggctc atcatctatg
     2281 attaatgctt tcatatttat tcctcctctt gtctttcata aggaagtaca caccaaaaag
     2341 tggtaccgct cgatgtcgat tcaaattgta atgctgcgga ttttccaaat aatcctttta
     2401 ggcgtaagtt taaattttct aaagcactac cagttccaga ctctgattct acagatgttt
     2461 ctcccaacaa atgcatttta tctttagaaa taccctgacc attatcttgt acaataatac
     2521 gtacatgtgt tgcagtttct ttaatcactg acacgtcaat atcgttacct tgctttcgat
     2581 tcgtaaacgc atgtttgatg gcattttcaa ctaaaatttg aattaaaaat ggtggtacaa
     2641 gcacatcgcg atacttgtct tcaacattaa tattgatatt aaatcttcct ggataacgtg
     2701 cttgttcgag tgataagtat gcacgcactt gacttaactc tttatctaaa gtaatcgtat
     2761 gttgctttga gccttgtaaa ttcgctctga aaaaataact caattctaat agtaactctc
     2821 gtgccttttc gctatttatt ctaactaaag ctgaaatcgt gttaattgaa ttgaagaaaa
     2881 aatgcggact cacttgtgcc tgcaatgact taatttcagc atctttcaat aacttacttt
     2941 gcgtttcggc ttcaccaagt tcaatttggc tactaaaaat atttgccaat ccttctgcaa
     3001 gttgacgttc cacaaaagtt aaatcattag ggtttgtaaa atacatcttc aatgtaccga
     3061 cgatagaacc atgcatctca agtggtatca cgatagctgc tctaagcggg caattcggat
     3121 gactacaacc aatctcttct ttagtatgaa cttctttcaa ctttcctgat ttcaatacat
     3181 ctttagacag acttgttaat atttcatttg ttggtatgtg atgatcacta cctgcaccta
     3241 catgcgataa gatttcattt ttgcttgtaa ttgctacggc agatactttc attaaatttt
     3301 taataatcat cgcaatttgc tgtgccgatt ctctattcaa tccttcttta aaatacggca
     3361 atgtctggtt catcaattgc agtacatcat gtgtttgaac agccttcatt tgctcctctt
     3421 gctttaatgt tgaaatgata atagacataa aaatcgccgt accaacgcta ttaacaataa
     3481 tcattggtag tgcaattaat gatatgaggt caaccgcata tgctttgtcg tgggaaaatg
     3541 ttaaaatgct caacatttga atcatttcca taacaattcc aatcatggca cttttcgcaa
     3601 tactcgggta acgcttgcgt ctttgagctt gtaaaccaaa ataaccagca attataccga
     3661 taaatataga tgagataaga taaacttgtg catccgcccc acccatatac actctgaaaa
     3721 tacctgaaat aacgccaaca aatagaccta caaaagggcc accaactaat cctgcgacac
     3781 ctattgtcaa tacacgtgtg ttagctaaag atacatcatc atctaaacgg aagtacacac
     3841 ttcctgacaa actatgttga tgatcgatga cgataccagt taaattagac attaaggcaa
     3901 acaaactgaa aataatacat aattgccaac gtgctttcca tgtacgtcga cgattcatta
     3961 agtttttaaa atatggaata ttcatcaaca cataggccaa aataataatt aaacctacac
     4021 gctcaagtaa taacattgtt agcgatagca cgatagcacc tcagtgaatt ataattatgt
     4081 ttattttatc ataatagtgc atgtaaaatt catgaataaa ttattaaaaa aacacgatac
     4141 tatttaatta cctataccat taaaatttca tatcaattct tatgctaagt tgacaatttt
     4201 ccttttcaaa ataatgacta tcatgtcact aaatttctgt taagtatagc gaaaaactgc
     4261 tgacattact ttgccaacag tttctaatcg cgttatttat gatacttttt taaataaaac
     4321 atgattctct aaatgaacat gctcatgtgt taacacttca agtgctttta atctctgata
     4381 aacgagtcgc caagtaccac atgcctctat cggtggttca tagcttgatg ttaaatcgct
     4441 cattttcact aacaattgtc ccgttgcaat gtgatcagat actaaatcat caatcactgt
     4501 attaatatcg tctactactt caccttgttc atatttaatg agttttggaa aatcaacatc
     4561 atcttctttt tgcatatgct ctaacatgcc atttttaaat gtatcgtatg ttgcttttaa
     4621 ctcaactaaa tatggatgat taggtccatg tactttcgat aactttgtca cataaggtgt
     4681 taaattttta aattcttctc ttagaggttc atgatatgct gcttgaatat attgaataag
     4741 tgatgaaaca tttaaaaatt taggatttaa cgaacctggt gtattcgttt gttcaacttc
     4801 attgagacgt tgtaataatt cgttcaaatc tacatttttc ttttctaaga ctgctgcttc
     4861 tatacttact tgtccgccac aacaaaaatc tattcccaca cttctaaaaa tatctgctgc
     4921 tttcggataa tcagttacta catctgctac tatgtcattt ttatttatca ttatagataa
     4981 cctcctcatt ttcagtgtat acaatttaat gtatttgaaa atgataaacg aggaaatgtc
     5041 ataatcatgt cataaacagc aaaataaaac cctcaaatca tgttagttca catagatttg
     5101 agggcattta ttatttattt tagcgagtaa gtcatatgtg cagtgtttaa cttaacaata
     5161 tttttgaagt ctcgcttctt aatatcttta ctaagtaaga tttccacttc gttttctgac
     5221 gtttggctta acattcccat caccatgtcg tcttttagca cgacactagc cataccttct
     5281 tcaaatacat aaccacgatc cagaccggtt aaaatgattt tgtttttacc ttttaacttc
     5341 attgagattg aatccgttaa tgtaatagct gtaaagtata acgaaatgtt attaaagtct
     5401 tttgtgaaat acggtgttaa tctataaaaa tcattattca aatgaattaa tgcacttgtt
     5461 tctgcagtat atgcatattt attacgttga ttaccaacac gcacttcaaa tacttccgta
     5521 cctatttgga cttctaagaa aacatcccaa attttttctt taggtaaatc cgctttaata
     5581 tcttctaaca taatgttaaa acgatattgt ttattcgcat tgacagttaa aggtacatac
     5641 attgtgtgct tcttgtttcg tgaatgtaat ttaagtctga catcataatg tccttcgcca
     5701 atcataaatt catttaacgt tagctctgta acaattttat gattatcaaa tttaaatgct
     5761 gtcattttca cttttggctt agcgatatca atgccttcaa agtatggttc gccttcattg
     5821 aattgaatgt ttaatttacc atctacaata tcaaaacggt agtattgacc ttgacggtaa
     5881 ctttcttcta cagttctata gttgtctata tctttcgctc tcgcgtagtg caacaatggt
     5941 cgtaatttac tcatcaccaa tgcatctaca cgttctggaa ctgcttgtat aaagtctcct
     6001 agagcattaa tccattgcgg ttgatcttct agtttaactt taagtgagaa gccattcgta
     6061 cgagaaaaac taaaatgacg atttaaaaat tctgctaaga tttgatcttt atgagcttct
     6121 tctaaatctg catttaatat ttctacagca atcaatctca tgacttcgta aaagtcttca
     6181 ggtgaaacat acgcactact catatgttcc ccttcacgct ttgtagcata ataatacgct
     6241 ttatcactta acacactgat tcgatttgca ttcaaatatg ctttcattgt aaataattga
     6301 tcttctgcac tctttaattc ttctggaaaa taaatgtcat tatcttttag taatgctgtt
     6361 ctatagattt tagtcgggct taaagtatag ataattcttg aatttaacaa tgtaacttca
     6421 ggtgctgttt ctttaaacat agattgtggt acaccacgac cattaacacc tttcatttta
     6481 atcaataaga catctgagtg atgttcatca ataaatgctg ctgcatcctt taaagtctct
     6541 ttgtttatat agtcatcgga gtccacaaat aatacaaatt caccttctgc ttgttttaac
     6601 gccacatttc taggtttacc tggaccacca gaatttgttt cgagttgact tactttcaag
     6661 ttcaatttat tgcgatactt ttcaactatt tgtaacgttt gatctgttga acagtcatca
     6721 actacaacca cttcaaattc agttttcgga aaatcttgtt tcgcaaggct attaagtaat
     6781 tctgttatat acttttctga attgtatgtt ggaactatta ctgaaaattt catcattata
     6841 cctctcccac tttgaatact atataaactt agctaccaaa taaatttctg actaaacgct
     6901 cacttgatcg gccatcttga tatttaaaat gtttatctaa gaatggaatg actttttctc
     6961 cttcataatc ttcattgtcc aaggcgtcca ttaatgcgtc aaatgattgc acaattttac
     7021 ctggaacaaa tgattcatat ggttcataaa aatcacgcgt cgtaatataa tcttctaaat
     7081 caaatgcata gaaaatcatt ggctttttaa atactgcata ttcatatatt aaagatgaat
     7141 agtcactaat taataaatct gttatgaaca gtatatcatt aacttctcta aagtcagaaa
     7201 cgtcaacaaa atattgttta tgtttgtctg caatattaag tctatttttc acaaatggat
     7261 gcattttaaa taatacaacc gcgttatttt tttcgcaata tcttgctaaa cgttcgaaat
     7321 caattttgaa aaatgggtaa tgtgctgtac catgaccact acctctaaat gttggtgcga
     7381 aaagaatgac tttcttacct ttaataattg gtaattcatc ttccatctct tgtttgatct
     7441 gtgtcgcata agcttcatca aatagtacat cagtacgtgg aacacctgta ggcactacat
     7501 ttttctcttt aataccaaat gcttcagcgt agaatggaat atcggtttca gatgatacat
     7561 aagcttttgt atagctacga tgatttaatg aatcaataaa tggtccaccc tttttaccag
     7621 tacgactaaa gccaactgtt ttaaaggcac caacggcatg ccatacttga ataacttctt
     7681 gagaacgtct aaaacgcact gtataaatca atgggtgaaa gtcatcaaca aagatgtagt
     7741 ctgccttccc aagtaaatat ggcaatctaa acttgtcgat gatgccacgt ctatctgtaa
     7801 tattcgcttt aaaaacagtg tgaatatcat actttttatc taaattttga cgtaacattt
     7861 cgttatagat gtattcaaag tttccagaca tcgttggtct agagtctgat gtgaacaaca
     7921 ccgtattccc ttttttcaag tggaaaaatt tcgtcgtatt aaatatcgct ttaaaaataa
     7981 attgtcttgt attaaatgat tgtttgcgga aatacttacg taattcttta tatttacgaa
     8041 cgatataaat acttttaact tccggagtcg ttacaacaac atcaaggaca aattcattaa
     8101 catcgctaga aatttcaggt gtaacagtat aaaccgtttt cttcgaaatg ccgccttttc
     8161 taaattcttt taggtaagtc tgcaataaga aattgatttt accattttgt gtttctaatt
     8221 cgttgtattc ttcttcttgt tctggcttta gattttgata tacatcatta atcacatctg
     8281 ggtttaactg tgcaatataa tcaagttctt gctcattcac taataagtac ttatcttcag
     8341 gtaagtaata accattatct aagatagcta cattgaaacg acaaacgaat tgattcccat
     8401 ctattttgac atcattcgcc ttcattgtac gtgtctcagt taaatttctt aatacaaaat
     8461 tactatcttc taaatctagg ttttcactat gtccttcaac gaataactga acacgttccc
     8521 aatagatttt atctatatat atcttacttt taaccaacgt taattcatcc ttttctattt
     8581 acataatcca ttttaatact gttttacccc aagatgtaga caggtctgct tcaaaagctt
     8641 ctgtaagatc attaattgtt gcaatttcaa attcttggcc ttttaacaac gctaatttat
     8701 ctacaatatc tgggtattga atgtataagt ctacaacatc ttggaaatct tttgaaccac
     8761 ttcgactact accaatcaac gttagtcctt tttccaatac tagacgtgta ttaacttcta
     8821 ctgggaactc acttacacct aacagtgcaa tgcttccttc tggtgaaatg taatcgatca
     8881 tttgatttat ggctgattga ctaccgcgac cacccacaca ctcaaatgca tgatcaaatg
     8941 ttaagccttc aggtatttta tcaataaaga agacatcatc aacaaatgag aagtgactca
     9001 atttataatc tgttttacca aagacatata ttttagactc tggatataat ttacgtaata
     9061 aaatggctgt aatgtaacct aagttaccat caccccaaat accaaatgta tttttatttg
     9121 aaatagattt cttttcaaaa cgacgaatag catgcaaact tactgttaca agctctgtat
     9181 atgaaataat acttaaatca atatcatcag gtagtggtac agctctatca tgatttagca
     9241 acacaaaatc ttgcataaac ccatcatgtc cacttgatct gaagtagctc gattttaaat
     9301 agttttcagc aatgacatcg tctttttctg tcggcgtatt cggtaccata actacttttg
     9361 tacctttatt aaacacacct ttactgtcaa atacgacctc accaacacct tcatgaatta
     9421 aagacattgg caatttctga gataagacat tctcatcacg gctaccagta taatatcttt
     9481 gatcggcagc acaaattgac atatataaag gacgtacaat gacatagtca ctgtaaatat
     9541 ctacgttgtt atacgtaact tcaaattgtc taggtgcaac taattgatat acttgattaa
     9601 tcatcggcaa taccacctcg aataatagca ttcgctactt ttaaatcgta aggtgttgtt
     9661 actttaatgt tatataactc accttttaca agtcgaaccg gtttgtttgt ttctacaata
     9721 atcttacaag catcagataa aatactcttt tgctcatcac tcaactgtgc atagctttct
     9781 tttaataaat ttatattaaa cgattgaggt gtttgacctt ggtacatttc attacgcact
     9841 ggaattgcat caatcgtttg attatcttta gatgtaacaa tcgtatctat agcatcaatc
     9901 actgtgtcta ctgcaccgta ctctaaagca gcttgaatat tttctttaat aatacgatgc
     9961 gttaaaaatg gtctaactgc atcatgtgtc acaataacat cgtcatcgtt aataccattt
    10021 gttgattcaa tatgtttaac gatattcata attgtatcgt tacgatcgct accaccttga
    10081 atgacttcaa ttctttcatc agaaattttg aattttctaa gtgtatcttt cgtatgcgtc
    10141 atccattgtt gtggcgtcgc gataataatt ttttcaaaat cattaattaa aataaatttt
    10201 tctaatgtat ggattaaaat cggtttgttg tctaaatcta aaaattgttt aggtaaaggt
    10261 acgttaccca ttcttgagcc tatacctcca gctagaatac cagcgtattt cataaaatac
    10321 ttcctccatt caactatatc tatatttaat tatttaaatt tcgttgcatt ttccaattga
    10381 aaactcattt taaaatcaaa actctaaatg tctatgtatt acttaaaatt atacatattt
    10441 tgcttatatt ttagcatatt ttgtttaaac ctatattaca ttatatcaga cgttttcata
    10501 cacaaataat aacatacaag caaacatttc gtttattatt tatatcactt aactaattaa
    10561 tttataattt tttattgttt ttaagttatc acttaaaaat cgtttggcaa attcgttgtg
    10621 acgcctttcc atcttctaat gaacagaatt tttgataaaa taccgttcgt gcttcaatat
    10681 actcatttgc agtctcatcg atttgtttta atgcatcaat gagtgctgtt tgattttcaa
    10741 caattggacc tggcaactct tttttataat ccatgtaaaa acctctaagc tcatcgccat
    10801 atttatctaa gtcatacgca tagaaaattt gcggacgctt taatacaccg aagtcgaaca
    10861 tgacagatga gtagtcggta actaacgcat cgctgattaa gtataaatcc gaaatgtctt
    10921 cataatctga aacgtctttc acaaaatcat catgttcatc aatacgtgtc acaactaaat
    10981 aatgcatgcg taataaaata acataatcat catccagcgc ttgacgcaaa gcttctatat
    11041 caaagttaac attaaattga tatgaacctt ctcgaatcgc ttcatcgtca cgccaagttg
    11101 gcgcgtacat aatcactttt ttatctaatg gaatatttaa tcttgtctta ataccattaa
    11161 tatattcagt atcattgcgt ttatgtgata atttatcatt tcttggataa cctgtttcca
    11221 aaatcttatc tcgactaaca tgaaatgcat gttgaaatat cgatgtcgaa tatggattag
    11281 gtgacactag ataatcccaa cgctggcttt cttttgtaaa gccatcttgg taattttgag
    11341 tatttgttcc tagcatttta acgttactaa tatccaaacc aatctttttc aatggcgtgc
    11401 catgccatgt ttgtaagtac gtcgttcgcg gtgatttata taaccaatct ggtgtacgtg
    11461 tgttaatcat ccacgctttc gctcttggca tcgctaaaaa ccatttcatt gaaaactttt
    11521 taacatatgg tacattgtgc tgttggaata tgtgttcata tccttttttc acaccccata
    11581 ttaattgggc atcactatgt tcagttaagt attcatataa tgctttgggg ttgtcgctgt
    11641 attgtttacc atgaaagctt tcaaaataaa ttagattctt gtttggcaat tttgatagta
    11701 atttaaatgt cgtatatata ctatgttcta tcaatttttt aattgtagtt ttaatcatgt
    11761 cgtacctccg acgtgttttt gtaattatat taatatgtat gagcaagctc attgtaacca
    11821 tgcttattat agcatttcat cataaaatac atttaaccat tacacttgtc gttaattatc
    11881 atacgaaata catgattaat gtaccacttt aacataacaa aaaatcgtta tccattcata
    11941 acgtatgtgt ttacacgttt atgaattaga taacgattgg attgattatt ttattttaca
    12001 aaatgacaat tcagttggaa gggtgattac ttttgattga atcgccttat gcatgaaaaa
    12061 tcaaaaggtt attctcattg tatagtcctg cttctcatca tgacatgttg ctcacttcat
    12121 tgtcagaacc cttcttgaaa actatgcctt atgactcatt tgcatggcaa gtaatatatg
    12181 ccaacattag cgtctaaaca aatctttgac taaacgttca cttgagcgac catcttgata
    12241 tttaaaatgt ttatctaaga atggcacaac tttttcaacc tcataatctt cattgtccaa
    12301 agcatccatt aatgcatcaa aggactgtac aattttacct ggaacaaatg attcatatgg
    12361 ttcatagaaa tcacgcgtcg taatgtaatc ttctaagtca aatgcataga aaatcatcgg
    12421 ctttttaaat actgcatatt catatattaa agatgaataa tcactaatca acaagtctgt
    12481 aacaaagaga atatcgttaa cttcacgatg atctgacaca tcgataaagt attgtctatg
    12541 ttcacgtgaa atattaagtc tattttttac gaacggatgc attttgaata acacaactgc
    12601 attatgcttc tcgcagtatc ttgctaaacg ttcaaaatca attttaaaaa atggataatg
    12661 tgccgtaccg tgaccattac ctctaaatgt cggtgcgaat agaataactt tcttaccttt
    12721 tataattggc aattcatctt ccatctcttg tttaatttgt gttgcataag cttcatcaaa
    12781 tagtacatca gtacgtggta cacctgttgg tacaacattt tcttctctaa ttccaaatgc
    12841 ttcagcataa aatggaatat cggtttctga tgaaacatat gctttcgtgt aactacgatg
    12901 gtttaatgaa tcgataaacg gaccaccttt tttacctgta cgactaaagc caactgtttt
    12961 aaaagcacca actgcatgcc acacttgaat aatttcttgt gatggtctaa agcgaaccgt
    13021 ataaattaat ggatggaaat catcgacaaa aatataatcg gccttaccaa gtaaatatgg
    13081 caatctaaac ttgtctctcc atttacgtct atccgtaata ttcgccttaa ataccgtttt
    13141 aatatcataa tcaaaatcta ctttttggcg tagtaactca tcatatacat atttaaagtt
    13201 acctgataaa ttcggacgtg aatctgatgt gaataatatt gttttgcttc ttttaatatg
    13261 tagtaacttt gtaatattaa aaatagcttt aaataagaaa cttctacttt caaatgaagc
    13321 tttatggcct tgtttatgaa gccagtgtgc gcttgtcgca atgacccctg atttctcttg
    13381 aggtaaggtg atttcaatat caaatacaaa ttcgttaacg tcacttgcta tttctggagt
    13441 aatggtataa accgtatgtt gtgatacgcc acctttgcgg aaaactttag cttcgttggc
    13501 taacacatag tttgcttttc cattttgtgt agtcagctca ttgtattctt caatttgctc
    13561 ttcatttaat gccgcataag cttgatcaat aatatttgga ttaagttgtc caatatactc
    13621 taactggtca tgataaacaa acaaatattt atccattggt aaataatacc catcgtctaa
    13681 tattgcgacg ttgaatctac atataaatgt attaccgtct attttcattt catttgcttc
    13741 taatgttttt gtttcggtta aattcctcag aacaaattgt cctgatgtta aatctacacc
    13801 ttcactatgt ccttcaatat ataactgaac acgttcccag tatatgttat caatatgtat
    13861 tgcttgtttc gtttttgtca ttgttattcc cccattggtt tattaaatcg tccatttcaa
    13921 tactgttttt ccccaagatg tcgataaatc catttcaaac gcttggacga tatcttgcat
    13981 cgtacataca ttaatttcat gtccttttaa taaggctaac ttttcaacaa tgtctgggta
    14041 cttatgatat aaatcaacaa cttgctcaaa atctttagag ccgcttcgac tactaccaat
    14101 caacgttaat cctttttcaa gtactaatcg tgtattcact tccacgggta attcacttac
    14161 gcctaacaaa gcaatactgc cttctggtga aatatgttca actatttgtt gaagtgcaac
    14221 ttgacttcct ttacctccaa cacattcaaa tgcatgatca attttaagat catctggtat
    14281 ttgatttact gtaaagatgt catctacaaa tgaaaaatga cttaatttat agtctgtctt
    14341 accaaataca taaattttag cttctgggta caacttacgt agcaaaatag cagtaatata
    14401 acctaagtta ccatcacccc aaataccaaa gctggttttc aaaggtatag atttacgttc
    14461 aaatcgttgt atagcatgat aacttactga cactaattct gtgtatgaaa tcgtactcaa
    14521 atcaatgtca ttaggcagcg gaacgatacg atcatgtgcc atcacaacgt agtcttgcat
    14581 aaaaccatca taaccactag atctaaaata actagaggct aagtaattct ccgcaataat
    14641 atgatgttgc tctgtaggtg tattcggcac cattactact ttcgtacctt tttcaaatac
    14701 ccctgtacta tcgaatacaa cttcaccaac agcttcatga actaatgaca ttggtaattt
    14761 ttggcgcagt acattttcat ctcttcgacc tgtgtaatac ctttgatcag ctgcacaaat
    14821 agacaagtat aaaggtctta cgatgacatg attaccataa atatcaacat tattatatgt
    14881 gacgtcgaac tgtctcggtg caacgagttg atatacttga ttaatcatcg gcaatatcac
    14941 cttgaataat ggcatttgct acttttaaat catacggtgt tgtcacttta atgttgtata
    15001 gttctccacg taccaattta actggatgtc cagattcgac aatgatttta catgcatctg
    15061 ataagatttc tttttgttca ctacttaagg cgcgataact atcttgtaat aatttaatat
    15121 taaatgattg tggtgtttgg ccttgataca tttcattcct tacagggata ctgtgtatgt
    15181 tctgtttatc tttagacatt acaatcgtat caattgcttc aatgactgta tctactgcac
    15241 catattttgc tgctacttca atattctctt taataatacg ttgagttaaa aatggtctta
    15301 cggcatcatg agttacaatc acatcatcat tattaattcc atttatattg cgaatgtggt
    15361 cgataatgtt cataattgtt tcgtttcgat ccgtgccacc tgcaactact ttgacacgtt
    15421 gatctgtaat gttatatttt tttaaaatat cctgtgtatg ggaaatccac tgtgctggcg
    15481 ttgcgataat aatctcatta aattcactca ctaaaataaa cttctcaatt gtatggatta
    15541 aaatcggttt attatcaata tctaaaaatt gttttggtaa tggcacgttc cccattctcg
    15601 aaccaatacc tcctgctaaa atacctgcat aaatcatgtt gtcctccatt ctgtcattac
    15661 atcatttcca tttatacatt actgacctat gcccgcacat aagcctaacc tattgctcac
    15721 ttgcctctat tattaagcca aaggtagttg tcacaatagt gtgataattt tttataaaaa
    15781 tgtatttttg taactgacca ttctaagttg ttttgccatg cagttaatca ttaacactga
    15841 cgatattaaa ttgttaaagg tattaatgtt tactcttttt caaattca
//
LOCUS       DAHHOJ010000060        15762 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.060, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000060 DAHHOJ010000000
VERSION     DAHHOJ010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15762
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.060"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            107..877
                     /locus_tag="NBH32_001624"
     CDS             107..877
                     /locus_tag="NBH32_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1515217.1"
                     /translation="MMKRLNKLVLGISLLFLVISITAGCGMGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDEFDKNDKGTWTISSEMAIQKKGEALNIKGMVLKLNRNARSA
                     KGFYYVNAIKKDENGRPLDNQIEYPVKMIDNKIIPTKDIKDEKIKKEIENFKFFAQYG
                     NFKDLSKYKDGDISYNPEVPSYSAKYQLTNDDYNVKQLRKRYNIPTNKAPELLLKGSG
                     NLDGSSVGYKKIEFTFVEKKGENTYFTANLHFKPSEDE"
     gene            942..1712
                     /locus_tag="NBH32_001625"
     CDS             942..1712
                     /locus_tag="NBH32_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1515218.1"
                     /translation="MMKRLNKLVLGISFLFLVISITAGCGIGKEAEIKKSFEKTLSMY
                     PIKNLEDLYDKEGYRDDQFDKNDKGTWIINSEMVVQPKGERMKSKGMVLYMNRNTKTT
                     TGKYIVSETLHDEDGRPKSKDKEYPVKMVDNKIIPTKGIKDENIKKEIENFKFFAQYG
                     NFKDLSKYKDGDISYNPEAPIYSAKYQLTNNDYNVKQLRKRYDIPTNKAPKLLLKGSG
                     NLKGSSVGYKKIEFTFVEKKGENIYFSDGLQFNPSEDK"
     gene            1810..4047
                     /locus_tag="NBH32_001626"
     CDS             1810..4047
                     /locus_tag="NBH32_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1515219.1"
                     /translation="MQRDYLIRVETESMSDFKRLNGLMIGFVIKGEAHIYDENNMTQC
                     NSGDIFIINHRDLYRFQLQQDGIICYIQFQMKYLADKFDDVHCLYFHLTDATTTKNIH
                     QLRNIMARLVSTHIRHNELSKLTEQQLVIQLLMHMIHYVPRTYHSNQSILNDDKVNQV
                     CDYIELHFHEDLSLSELSEYVGWSESHLSKKFAESLGVGFQHFLNTTRIEHAKLDLTY
                     TDETITDIALQNGFSSAASFARTFKHFTHQTPKQYRGDRPAITENQQSAQHNYHDREL
                     ILLLNDYIEEMNHFIEDIEKMNYKEIAFKPTNQQLNQFNHIIQVGYLRNLLNTQYQSQ
                     LLTCHHDFHVNEVLAYDVMPYIMKKLNAPFTYDAEISNIFYDIDLCLDFLLDHNFSLT
                     MHLDQYDSRDYIDAFKVFIHHVALHVSHRKDLKFNLYVTTLHTSLIEMIDYFKALFPN
                     GGLYIHLDQATERHLPLLKRLEPHINHFVFDANSNDAVDFNKMNDDEFKTASQIIINK
                     TNYLIDLMHRHNLKRPLILLNWNTLTGDTFITNGEYFRGGIIIEQLLKLSSKVEGIGY
                     WLNYDLHVSHCRNERDYMNSIELFHQYNGKRPVYFTALLFNKLTSNILYSDDTCIVTG
                     TDSNFQILLYDAKHFNPYLALDNQMNMRATEMIHLNINALEDGMYKIKHFTLDKENGA
                     LFNLWRKHHTIHGMDKDSIDYVNRMSFPKLEVYDIDITETLALNIKMITNGIHLIEVK
                     RYPSS"
     gene            4198..5376
                     /locus_tag="NBH32_001627"
     CDS             4198..5376
                     /locus_tag="NBH32_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1515220.1"
                     /translation="MNQQLIETLKSKEGKMIEIRRYLHQHPELSFHEDETAKYIAEFY
                     KGKDVEVETNVGPRGIKVTIDSGKPGKTLAIRADFDALPITEDTGLSFASQNKGVMHA
                     CGHDAHTAYMLVLAETLAEMKDSFTGKVVVIHQPAEEVPPGGAKAMIENGALDGVDHV
                     LGVHVMSTMKTGNVYYRPGYVQTGRAFFKLKVQGKGGHGSSPHMANDAIVAGSYFVTA
                     LQTVVSRRLSPFETGVVTIGSFDGKGQFNVIKDVVEIEGDVRGLTDATKATIEKEINR
                     LSKGLEDMYGVTCTLEYNDDYPALYNDPEFTEYVAKTLKEADLEFGVEICEPQPPSED
                     FAYYAKERPSAFIYTGAAVEDGEIYPHHHPKFNISEKSLLISAEAVGTVVLDYLKGDN
                     "
     gene            5378..6766
                     /locus_tag="NBH32_001628"
     CDS             5378..6766
                     /locus_tag="NBH32_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515221.1"
                     /translation="MNETYRGGNKLILGIVLGVITFWLFAQSLVNVVPNLQQSFGADM
                     GTISIAVSLTALFSGMFVVGAGGLADKIGRVKMTNIGLLLSIIGSALIIITNLPALLI
                     LGRIIQGVSAACIMPSTLAIMKTYYEGAERQRALSYWSIGSWGGSGICSLFGGAVATT
                     MGWRWIFIFSIIVAVLSMLLIKGTPETKSEVTNTHKFDVAGLIVLVVMLLSLNVVITK
                     GAALGYTSLWFFGLIAIVIVAFFIFLKVEKKVDNPLIDFKLFENKPYTGATISNFLLN
                     GVAGTLIVANTFVQQGLGYTALQAGYLSITYLIMVLLMIRVGEKLLQKMGSKRPMLLG
                     TFIVVIGIALISLVFLPGIFYVISCVVGYLCFGLGLGIYATPSTDTAISNAPLDKVGV
                     ASGIYKMASSLGGAFGVAISGAVYAGAVAATSIHTGAMIALWVNVLMGIMAFIAILFA
                     IPNDDKRVKDAK"
     gene            complement(7253..8914)
                     /locus_tag="NBH32_001629"
     CDS             complement(7253..8914)
                     /locus_tag="NBH32_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCD1515222.1"
                     /translation="MSVTEVIFSFLGGLGIFLYGLKIMGDGLQASAGDRLRDILNKFT
                     SNPVLGVIAGIVVTILIQSSSGTTVITIGLVTAGFMTLKQAIGVIMGANIGTTVTAFI
                     IGIDLGEYAMPILALGAFLIFFFKRSKINNIGRILFGFGSLFFGLEFMGDAVKPLASL
                     DGFKQLMLDMSTNPILAVIVGAGLTALVQSSSATIGILQEFYQQDLISLNAAIPVLLG
                     DNIGTTITAILASLAGSIAAKRAALVHVIFNLIGVIIFTIFLPVVIHLISLLQDVWHL
                     KPAMTIAVSHGIFNITNTLIQLPFVAGLAWIVTKLVPGKDIADDYKPQHLNKDLVYHA
                     PGVALQETQKELQNVGQIVLSMFEDIREITKDDKKLIKKLEQKHQAVETINDSIRNYL
                     VRISTKAITKADVERLAVMFDVNRSILKVAELTEEYVAQLKRQHDEDIRITEDAQRGM
                     DKLFNHVAESFDKAIDMLDVYDKTKKDEIVERSRESFNIEHKLRKGHIKRLNRGECTT
                     KGGLLYIDMIGVLERIGYHSRNVSEALVGLNDDVPTDEEIATTEI"
     gene            complement(9241..11016)
                     /locus_tag="NBH32_001630"
     CDS             complement(9241..11016)
                     /locus_tag="NBH32_001630"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HCD1515223.1"
                     /translation="MYYSYGNYEAFARPKKPENVENKSAYLIGSGLASLAAACFLIRD
                     GQMEGSKIHILEELPKAGGSLDGENMPLKGYVVRGGREMENHFECLWDLFRSIPSLEI
                     DNASVLDEFYWLNKEDPNYSRCRVIEKQGQRLVTDGDFTLTKKAIKEILDLCLTNEED
                     LDDVKITDVFSDDFFNSNFWIYWKTMFAFEPWHSAMEMRRYLMRFVHHIGGLADFSAL
                     KFTKYNQYESLVLPMVEYLKSHGVQFEYDVKVEDIKVDVTTSQKIAREILIHRHGKAE
                     SIKLTVDDLVFVTNGSITESSTYGDNDTPAPPTDELGGSWTLWKNLARQSPEFGNPDK
                     FCQNIPQKSWFVSATSTTNNKDIIDTIESICKRDPLAGKTVTGGIITINDSAWQISFT
                     INRQQQFKDQPKNEISTWIYALYSDVNGDYIKKPITECSGNEICQEWLYHLGVPTDKI
                     EDLAKHASNTIPVYMPYITSYFMTRAIGDRPLVVPHQSQNLAFIGNFAETERDTVFTT
                     EYSVRTAMEAVYQLLNINRGIPEVINSTFDLRVLMDAIYELNDHQDLREITKDSKMQK
                     LALAGFLKKIKGTYIETLLKEHKLL"
     gene            11367..11840
                     /locus_tag="NBH32_001631"
     CDS             11367..11840
                     /locus_tag="NBH32_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1648 domain-containing protein"
                     /protein_id="HCD1515224.1"
                     /translation="MSKIRSFTILSLLIYLAMTCYTVVTYPKLPTKVPIHYNLAGDAD
                     NFADKWVLLLINSAFIVIWLIFFIAGRYYERFAKWSHYNHTPREIRAIKLFLSTLNLE
                     IMSYMSIFTVLEIWQIQHHHQFNLLWFNMIFIIIIGLTLVIFCLLPTIHKMRDSQ"
     gene            12102..13694
                     /locus_tag="NBH32_001632"
     CDS             12102..13694
                     /locus_tag="NBH32_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCD1515225.1"
                     /translation="MTLLTVNPFDNVGLSALVAAVPIILFLLCLTVFKMKGIYAALTT
                     LVVTLIVALFVFELPARVSAGAITEGVVAGIFPIGYIVLMAVWLYKVSIKTGQFSIIQ
                     DSIASISEDQRIQLLLIGFCFNAFLEGAAGFGVPIAICAVLLIQLGFEPLKAAMLCLI
                     ANGAAGAFGAIGLPVSIIDTFNLSGGVTTLDVARYSALTLPILNFIIPFVLVFIVDGM
                     KGIKEILPVILTVSGTYTGLQLLLTIFHGPELADIIPSLATMVVLAFVCRKFKPKNIF
                     RLEASEHKIQKRTPKEIVFAWSPFVILTAFVLVWSAPFFKKLFQPGGALESLVLKMPI
                     PNTVSDLSPKGIALRLDLIGATGTAILLTVIITILITKLKWKSAGALLVEAIKELWLP
                     ILTISAILAIAKVMTYGGLTVAIGQGIAKAGAIFPLFSPVLGWIGVFMTGSVVNNNTL
                     FAPIQATVAQQISTSGSLLVAANTAGGVAAKLISPQSIAIATAAVKKVGEESALLKMT
                     LKYSIIFVAFICIWTFILTLIF"
     gene            complement(14023..15693)
                     /gene="spa"
                     /locus_tag="NBH32_001633"
     CDS             complement(14023..15693)
                     /gene="spa"
                     /locus_tag="NBH32_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal protein A"
                     /protein_id="HCD1515226.1"
                     /translation="MKKKNIYSIRKLGVGIASVTLGTLLISGGVTPAANAAQHDEAQQ
                     NAFYQVLNMPNLNADQRNGFIQSLKDDPSQSANVLGEAQKLNDSQAPKADAQQNNFNK
                     DQQSAFYEILNMPNLNEAQRNGFIQSLKDDPSQSTNVLGEAKKLNESQAPKADNNFNK
                     KQQNAFYEILNMPNLNEEQRNGFIQSLKDDPSQSANLLSEAKKLNESQAPKADNKFNK
                     EQQNAFYEILHLPNLNEEQRNGFIQSLKDDPSQSANLLAEAKKLNDAQAPKADNKFNK
                     EQQNAFYEILHLPNLTEEQRNGFIQSLKDDPSVSKEILAEAKKLNDAQAPKEEDNKKP
                     GKEDGNKPGKEDGNKPGKEDNNKPGKEDGNKPGKEDGNKPGKEDNKKPSKEDGNKPGK
                     EDGNKPGKEDNKKPSKEDGNKPGKEDGNKPGKEDGNKPGKEDGNKPGKEDGNKPGKED
                     GNKPGKEDGNGVHVVKPGDTVNDIAKANGTTADKIAADNKLADKNIIKPGQELVVDKK
                     QPANHADANKAQALPETGEENPFIGTTVFGGLSLALGAALLAGRRREL"
BASE COUNT         5076 a         2470 c         2898 g         5318 t
ORIGIN      
        1 gattgtttat ggaatttttg ataatattaa agtgaaaaag tgttataaat tgaaattaat
       61 gtgtaatttg caaaagtaaa tcattttaaa agaagagagt tgtaagatga tgaaacgatt
      121 aaacaaatta gtgcttggta ttagtcttct gtttttagtc attagtatca ctgctggttg
      181 tggcatgggt aaagaagcgg aaattaagaa aagttttgaa aaaacgttga gtatgtatcc
      241 gattaaaaat ctagaggatt tatacgataa agaaggctat cgtgatgacg aatttgataa
      301 aaatgataaa ggaacatgga ctattagttc tgaaatggca attcagaaaa agggagaagc
      361 cttaaatata aaaggtatgg ttttgaagtt aaatagaaat gcaagaagtg caaaaggatt
      421 ttactatgtt aatgcgataa agaaggatga aaatggcaga cctctggata atcaaataga
      481 atatcccgtt aaaatgattg acaataaaat cattccaact aaagatatca aagatgaaaa
      541 aataaaaaaa gaaatagaaa actttaagtt tttcgcgcag tatggcaact ttaaagattt
      601 atcgaagtac aaagatggag atatttcata taatccagag gtgccgagtt attcagccaa
      661 atatcaatta actaatgatg attataatgt aaagcaatta cgtaaaagat ataatatacc
      721 aacgaataaa gcgccggagt tattattgaa aggttcagga aatttagatg gttcatcagt
      781 tggctacaaa aaaattgaat ttactttcgt agagaaaaaa ggagaaaaca cttactttac
      841 agctaaccta cattttaaac cgagtgagga tgaataatcg tgactaataa agagtatgaa
      901 atcgattcaa ggaaacaaaa taaaaagaag agagttgtaa ggtgatgaaa cgtttaaata
      961 aattagtgtt aggtattagt ttcttatttt tagtcattag tattactgct ggttgtggca
     1021 taggtaaaga agcggaaata aagaaaagtt ttgaaaagac attgagtatg tatccaatta
     1081 aaaatctaga ggatttatac gataaagaag gctatcgtga tgatcagttt gataaaaatg
     1141 ataaaggcac atggattatt aattctgaaa tggttgttca acctaaaggg gaacgtatga
     1201 aatcaaaagg tatggttcta tatatgaata gaaacactaa gactacaacc gggaagtata
     1261 tagtgagtga aacacttcat gatgaagatg gtagacctaa gagtaaagat aaagaatatc
     1321 ccgttaaaat ggtagacaat aaaatcattc caactaaagg tatcaaagac gaaaacataa
     1381 aaaaagaaat cgaaaacttt aagttcttcg cgcaatatgg caactttaaa gatttgtcga
     1441 agtacaaaga tggagacatt tcatataatc cagaagcgcc gatttattca gctaaatatc
     1501 aattaactaa taatgattat aacgtaaagc aattacggaa aagatatgat ataccgacga
     1561 ataaagcacc aaagttattg ttgaaaggct ctggaaattt aaaaggttca tcagtcggat
     1621 acaaaaaaat tgaatttact ttcgtagaga aaaagggaga aaatatatat tttagtgatg
     1681 ggctacaatt taacccaagt gaggataaat aacctcactg cctcccacac gattcaatat
     1741 gaaattcaaa tatgttgctg taaaataatg taaaataaac gtattcatat tactgaattg
     1801 agggatagta tgcaacgtga ttatttaatt cgagtagaaa ctgagagtat gtcagatttc
     1861 aaaaggctca atggtttaat gattggtttt gttattaaag gtgaggcgca catttatgat
     1921 gaaaataata tgacgcaatg caacagtggc gacattttca tcattaacca ccgcgacttg
     1981 tatcgatttc aacttcaaca agatggcatc atatgttata tccaattcca aatgaaatat
     2041 ttagcagaca agtttgatga tgtgcattgt ctatattttc acttaacaga tgcgaccaca
     2101 accaaaaata tacatcaact gagaaatata atggcaagac tggtttcaac acatattcga
     2161 cataatgagt tgtctaaatt gactgagcaa caacttgtga ttcagttgct tatgcatatg
     2221 attcattatg tcccgcgtac atatcattcg aaccaaagta tcttaaatga tgataaagtg
     2281 aatcaagtat gcgactatat cgagttacat tttcatgaag acttaagcct ttcggaatta
     2341 agcgaatatg ttgggtggtc agagagccat ctgtctaaaa agtttgcaga atcgctaggt
     2401 gtaggattcc agcatttctt aaatacgacg cgaattgagc atgcgaaact cgatttgaca
     2461 tacacagatg aaacgattac tgatattgca ttgcaaaatg gcttttcaag tgcagcgagc
     2521 tttgcgagaa catttaaaca ctttacgcat caaacgccta aacaatatcg aggtgatcgt
     2581 ccggcaatca ctgaaaatca acaatcggca caacataatt atcacgaccg tgaattgata
     2641 ttacttttaa atgactacat tgaagaaatg aatcatttca ttgaagatat tgaaaagatg
     2701 aactataaag agattgcctt taaaccaact aatcaacaac taaatcaatt taatcatatt
     2761 attcaagtgg gctatttgag gaatttgctc aatacacagt atcaatcaca gttacttaca
     2821 tgccatcatg attttcatgt caatgaagta ttagcatatg atgtgatgcc atatattatg
     2881 aaaaagctca atgcgccatt cacgtatgat gcagagattt cgaatatatt ttatgatatc
     2941 gatttgtgtt tagacttttt attagatcat aactttagtt tgacaatgca tttggatcag
     3001 tatgactcac gtgattatat cgatgcattc aaagtattta tccatcacgt tgccctgcat
     3061 gtcagtcata gaaaagattt gaagttcaac ttgtatgtga cgacattgca cacttctttg
     3121 attgaaatga ttgattattt taaagcgtta ttccctaatg gtggcttgta cattcactta
     3181 gatcaagcta cggaaagaca tctaccattg ttgaaacgac ttgagccaca catcaaccat
     3241 tttgtatttg atgccaattc aaatgatgct gttgatttta ataaaatgaa tgatgatgaa
     3301 tttaaaaccg cgagtcaaat aattattaat aaaacgaatt accttatcga cttaatgcat
     3361 cgccataacc taaagagacc acttatttta ctcaattgga atacattgac gggtgataca
     3421 tttattacaa acggcgaata ttttagaggt ggtatcatca ttgagcaatt attaaaatta
     3481 agttctaaag tagagggtat cgggtattgg ttgaattatg atttgcacgt cagtcattgt
     3541 agaaatgaac gggattatat gaattctatt gaactgtttc atcaatataa tggaaaacgt
     3601 ccggtctatt tcacggcatt gctatttaat aaattaacaa gcaatatttt gtattctgat
     3661 gatacatgta ttgtcacggg gactgattca aattttcaaa tattgttata tgatgcaaag
     3721 cattttaatc cgtacttagc gttggacaat caaatgaata tgcgtgcaac ggaaatgatc
     3781 catttgaaca ttaacgccct agaagacggt atgtataaga ttaaacattt taccttagat
     3841 aaagaaaatg gtgcgttatt taatctttgg cgcaaacatc atacgataca tggcatggac
     3901 aaggactcta tagattacgt taatcgaatg agttttccga aattagaagt atatgatata
     3961 gatatcacgg agacactggc attaaacatt aaaatgatta cgaatgggat tcacttaatt
     4021 gaagtaaaac gttacccaag ttcataaaat gatcacaaat cacaaatttt gatatacata
     4081 atttgtgatt ttttatattc aaagctaaaa ttgcaaaaaa ttaatggtta acatctctgt
     4141 tgttgtcaat ataaataatg attaatcatt tatgatgtaa ctaaggagat gaaggatatg
     4201 aatcaacaat taattgaaac tttaaaatct aaagaaggca aaatgattga gatcagacgt
     4261 tatttacatc agcatccaga attatctttt catgaagatg aaacggcgaa atacatcgct
     4321 gaattttaca aaggtaaaga tgtggaagta gaaacgaatg tcggaccacg tggaattaaa
     4381 gtaacgattg attcagggaa acctggtaaa acattagcaa tccgtgcaga ctttgatgca
     4441 ttacctatta ctgaagatac aggattatct tttgcatcac aaaataaagg tgttatgcac
     4501 gcatgtggtc acgatgcaca tacagcatac atgcttgtat tagcagagac gcttgctgaa
     4561 atgaaagata gttttacagg aaaagtcgtt gtgatacatc aaccagctga agaagtacca
     4621 ccaggtggtg ctaaagcaat gattgaaaat ggtgcattag acggtgttga tcatgtatta
     4681 ggtgtacacg tcatgagcac aatgaaaaca ggtaatgtgt attacagacc tggttatgtt
     4741 caaacaggac gcgcattctt caaattgaaa gttcaaggta aaggtggtca tggttcatca
     4801 ccacatatgg ccaatgatgc cattgttgca ggtagctact tcgtcacagc gttacaaaca
     4861 gttgtatcta gacgactaag tccatttgaa accggtgttg tcacaatcgg ttcatttgac
     4921 ggtaaaggtc aattcaatgt cattaaagat gttgttgaaa ttgaaggtga tgtacgtgga
     4981 ttaacagatg ctacaaaagc aacaattgaa aaagaaatta accgtttatc aaaaggctta
     5041 gaggatatgt atggtgtaac ttgcacctta gaatataacg atgattatcc tgcattatat
     5101 aatgatccag agtttactga gtacgtggct aagacgttaa aagaagcaga ccttgaattt
     5161 ggtgtcgaaa tatgtgaacc acaaccacct tcagaagact ttgcatacta tgctaaagaa
     5221 cgtccaagtg cctttattta tacaggtgca gcggtggaag atggtgaaat ttacccacat
     5281 catcatccta aatttaacat ttcagaaaaa tcattactta tttcggcaga agctgtaggg
     5341 acagttgttt tagattacct taaaggagat aactaacatg aatgaaacgt atcgcggggg
     5401 caacaagtta atcttaggta ttgtattagg tgttattaca ttttggttgt ttgcacaatc
     5461 acttgtaaat gttgtaccaa atttacaaca aagttttggt gcagacatgg gaacaattag
     5521 tattgcggta agtctaaccg cactattttc aggcatgttt gttgttggag caggcggcct
     5581 agcagataaa attggacgcg tgaaaatgac aaatatcggc ttattattaa gtattattgg
     5641 ttcagcatta attattatta caaatttacc agcattgtta attttaggtc gtattataca
     5701 aggcgtatca gcagcgtgta ttatgccttc cacattggcc attatgaaaa cttattatga
     5761 gggtgctgaa cgtcagcgcg ccttaagcta ctggtctatc ggttcttggg gtggaagtgg
     5821 tatctgttca ctcttcggtg gggcagttgc gacaactatg ggttggagat ggattttcat
     5881 cttctcaatt atcgttgccg tactttcaat gttactcatc aaagggacgc ctgaaacgaa
     5941 atcagaagtt accaatacac ataaatttga cgttgcaggg ctaattgttc tagtagttat
     6001 gttgctaagt ttaaacgttg tcattactaa aggtgcagca cttggttaca catcattatg
     6061 gttctttggt ttgattgcaa tcgtaattgt agcattcttt attttcttaa aagttgagaa
     6121 aaaagtagat aatccgctta ttgattttaa attatttgaa aataaaccat atacaggtgc
     6181 aacgatttcg aacttcttat taaacggtgt tgcaggtaca ttaattgtag cgaatacatt
     6241 cgtgcaacaa ggtttaggtt atacagcatt gcaggcagga tacttatcaa tcacttattt
     6301 aattatggtg ttattgatga ttcgagttgg tgaaaaatta ttacaaaaaa tgggttctaa
     6361 gcgaccaatg ttattaggta cattcattgt ggtcattggt attgcactta tttcattagt
     6421 attcttacca ggcatatttt atgttatcag ttgtgtcgta ggatatttat gtttcggact
     6481 aggcttaggt atttatgcaa caccttctac agatacagct atttcgaatg caccgttaga
     6541 taaagttggc gttgcttcag gtatttataa aatggcttca tcacttggtg gtgcattcgg
     6601 tgtcgcaatt agtggtgcag tatatgccgg tgcagttgct gcaacgagca ttcatacagg
     6661 tgcgatgatt gcactttggg ttaacgtatt aatggggatc atggcattta tcgcaatttt
     6721 attcgcgatt cctaatgatg ataaacgtgt caaagatgcg aaataataac gatgacacgc
     6781 ccagtgtttt gataataatt taaatgaaag taaagcaaat taaatgagga aatgtataga
     6841 gcagtgcata tagataaatc tatgattgga ttaacactaa tataaatata aatgaaaaac
     6901 catccactta aacttttaat aagttaggtg gatggttatt tattgcgttg ttctaaaaat
     6961 tttgacactt atatggaaag aaatatatat aatgtgaagg ttatggcaat tcgcttcaat
     7021 gaaggcgacg tgtaaaagtg tatattaaat gattcgaggt cagactccga gaacagtgtc
     7081 cgtcatgctt accgcatact aatgaagcat cattatatca gtgtttatag ccgatttaag
     7141 ataataaatt ttgctaaaca aaaaagccaa cccatgaatg ttggattggc tttttacatg
     7201 catctgaatc tctaatttta aaaaaatatg aatataaata agatagtaaa aattaaattt
     7261 cagttgttgc aatttcttca tctgtaggta catcatcgtt aaggccaaca agtgcttcag
     7321 aaacatttcg tgaatgataa ccgatacgtt caagaacacc aatcatgtcg atatatagta
     7381 atccgccttt tgttgtacat tcaccacgat taaggcgttt aatatgacct ttgcgtagtt
     7441 tatgttcaat attaaatgat tctctactac gttctacaat ttcatctttt ttcgttttgt
     7501 cataaacatc taacatgtcg atggctttat caaatgattc ggcaacatgg ttgaataatt
     7561 tatccatacc gcgttgtgca tcttctgtaa tgcgaatatc ttcatcatgt tggcgtttta
     7621 attgagcgac atactcttct gttagctctg ctacttttaa aatagagcga ttgacatcaa
     7681 acataactgc taaacgctca acgtctgcct tcgtaatggc ttttgtagaa attctaacta
     7741 aataatttcg aatactatca ttgattgttt caacagcttg gtgcttttgt tcaagctttt
     7801 tgatcaattt tttatcgtct tttgtaattt cgcgaatgtc ttcaaacatt gataagacaa
     7861 tctgaccaac attttgtaat tctttttgag tttcttgtaa tgcaacacca ggtgcgtgat
     7921 aaacaagatc tttgtttaag tgctgaggtt tatagtcatc agcaatatct ttacctggga
     7981 caagctttgt aactatccat gctaaacctg ctacaaatgg taattgaatc aaagtatttg
     8041 ttatgttgaa gataccatgt gatactgcaa tcgtcatcgc tggttttaag tgccatacat
     8101 cttgtaacaa actaatcaaa tgaatcacaa ctggcaagaa aattgtgaag ataattaccc
     8161 cgattaagtt aaagatgacg tgtacaagcg ccgcacgttt tgcagcgatt gagccggcta
     8221 aactagctaa gatagctgta atcgttgtac caatattatc gcctagtaac acagggattg
     8281 ctgcgtttaa gctgattaaa tcttgttgat aaaattcttg taaaatacca atcgtcgcac
     8341 ttgaactttg aactagtgct gttaaccctg cgccgacaat gacagcaagt attggatttg
     8401 tagacatatc aagcattaat tgcttaaatc catctaatga tgctaaaggt ttaacggcat
     8461 cacccataaa ttctagaccg aagaatagag aaccgaaacc gaatagtatg cggccaatgt
     8521 tattgatttt agagcgctta aagaaaaaga ttaaaaatgc acctaatgct aaaattggca
     8581 ttgcatattc gcctaaatct ataccgataa taaatgcagt taccgttgtt ccgatattag
     8641 cacccattat cactccaatg gcttgtttca atgtcataaa tccagctgtt accagtccga
     8701 ttgtgataac tgtcgtacct gaactacttt gtattaaaat agttacaacg atacctgcaa
     8761 taacacctaa tactggattt gatgtaaatt tgtttaaaat atctcgtagc ctgtctcctg
     8821 ctgatgcttg aagcccgtct cccatgattt ttaagccgta aaggaaaata cctaaaccac
     8881 ctaaaaagga gaaaatgact tctgtaaccg acatttccat tatttcacct caaataagct
     8941 ttatatttag attatcgctt ataattgtaa atttaatgtt aagattaggt aaaattattt
     9001 aacaatatat gttatttgta tatgacttgt aaaatatcat cacttattat gtaaattttc
     9061 agtgtgaaat ggcaggtttg caatcacttg tttaacaaaa tgatgcaatc aatcatgtga
     9121 caatgttttg gcaataaaaa atcatgagag tggaacaacg aaataatttt tatgaaaaca
     9181 tcatttctgt cccagtctca tgattcgaaa tcaccaaata aaattattta atgtgcctcg
     9241 ttataacaat ttgtgttctt ttaataatgt ctcaatgtac gtacctttta tctttttaag
     9301 gaatcctgct aatgcgagtt tttgcatttt cgaatcttta gtaatctcgc gcaaatcttg
     9361 atggtcattc agttcgtata tggcatccat taagacgcga agatcaaatg tactattgat
     9421 gacctctgga ataccacgat ttatatttag taattgataa acagcttcca tggcagtacg
     9481 aaccgaatat tctgttgtaa atacagtatc tcgctctgtt tctgcaaagt taccaataaa
     9541 tgctaaattt tgtgattgat gcggaacgac taaaggtctg tcgccgatag cacgcgtcat
     9601 gaaataagag gtaatatatg gcatataaac aggaatcgta ttagatgcat gttttgctaa
     9661 gtcttcaatt ttgtcagttg gtacacctaa gtgatacagc cattcttgac atatttcatt
     9721 accactacat tctgtgattg gctttttaat ataatcgcca tttacatctg aatataaggc
     9781 ataaatccat gtagatattt catttttagg ttggtcttta aactgttgct gacgattgat
     9841 tgtaaaactg atttgccatg cagaatcatt gattgtaata attccaccag taaccgtttt
     9901 gcctgcaagt ggatcacgtt tacaaatgct ttctattgta tcgataatat ctttattgtt
     9961 agttgtcgat gttgcagaaa caaaccaact tttttgagga atattttggc aaaacttatc
    10021 aggattacca aattcaggac tttgtcgcgc taaatttttc catagtgtcc aactaccacc
    10081 taattcgtca gttggtggtg ctggtgtatc attatcacca tacgtagagc tttccgtaat
    10141 actaccgttt gtcacaaaga caagatcatc tacagtcagt ttaattgact ctgctttgcc
    10201 atgtcggtga attaatattt ctcgggcaat tttttgactt gtcgtaacat ctactttaat
    10261 atcttctact tttacatcgt attcaaattg aaccccatgc gattttaaat attcaaccat
    10321 aggtaatact aaagattcat attgattata tttagtgaat tttaaagctg aaaagtctgc
    10381 gagaccacca atatgatgaa cgaatcgcat tagatagcga cgcatttcca ttgcagaatg
    10441 ccacggttca aatgcaaaca tcgttttcca gtaaatccaa aagtttgaat taaagaagtc
    10501 atcggaaaat acatctgtta ttttgacatc atctaaatct tcttcattcg ttaagcataa
    10561 atctaaaatt tctttaatcg cttttttggt caaagtgaag tctccatcgg taactaaacg
    10621 ttgaccctgt ttttcaataa ctcgacagcg agaatagtta gggtcttctt tgtttagcca
    10681 atagaactca tctaatacag acgcgttatc gatttctaat gaagggatag atctgaataa
    10741 gtcccacaaa cattcaaagt ggttctccat ttcacgacca cctcggacaa catagccttt
    10801 taaaggcata ttttcaccat caagactacc acctgcttta ggtaactctt ctaaaatatg
    10861 aatcttcgaa ccttccattt gaccatccct aattaagaaa caagctgccg caagtgaagc
    10921 taacccagac ccgattaaat aagcagattt gttttctaca ttttcaggtt ttttagggcg
    10981 cgcaaatgct tcataatttc cataactgta atacatatcc accaagtcct ttcgtattag
    11041 aatacactca gactataccc ctttgtgaat tgcaaatatt agtatttata ttggtaaatt
    11101 tgacgaaatt agatcatttt tatatttttg attttaaaaa ttttatcact cgaatgtttt
    11161 tggattctga ggtgcgggac ttggaataat tcgaatcatt atgtggcatg ttaaacagct
    11221 aaatataagc tatgcacatt taacaagcgg atactcctga cgcaattata gtatcattac
    11281 atattgttta atcgtttggg gtaatgtaat taaaaggtaa aataggcaga gtgttataca
    11341 taaaatgtaa agaaagaggc gatagaatgt ctaaaatcag gtcttttaca atattaagtc
    11401 tacttattta cttagctatg acgtgctata cagtagtgac ctatccaaaa ttaccaacca
    11461 aagtgcctat tcattataat ttagcagggg atgctgataa ctttgctgat aaatgggtac
    11521 tgcttttgat taatagtgca tttatagtga tttggcttat atttttcatt gcaggtagat
    11581 actatgaacg atttgccaaa tggtcacatt ataatcatac accacgtgaa attcgagcga
    11641 ttaaattatt tttaagtacg ttaaatttag agattatgag ctatatgtct atcttcacag
    11701 tattagaaat ttggcaaata caacaccatc atcaattcaa tttactatgg tttaatatga
    11761 tatttatcat tatcattggt ttgacgcttg tcatattttg tttacttcct acaattcata
    11821 aaatgagaga ttctcaataa aaatataaac tgtcatgatg cagttttgaa gcggattcct
    11881 gaaattatta taaataatat ctagtattta taattaaaat gatatattat ttattatagt
    11941 taaatattgt gtatattttg tgaacttttt gtaaaaattc gattgcctgt cacatatagg
    12001 agtgttacat tttaaatatg tgatcatcgc aaaatataag ttgaaatagg ttgtagatta
    12061 atcagaatga taaatatttt atataaagag agggagtcat tatgacacta cttactgtaa
    12121 atccattcga taatgttgga ttatcagcct tagttgcagc agtacctatt attttatttt
    12181 tattatgctt aaccgttttt aaaatgaaag gcatttatgc agcattgaca actttggttg
    12241 ttacattgat tgtggcttta tttgtatttg aattaccagc gcgtgtatca gcaggtgcga
    12301 ttacagaagg cgttgttgcc ggtatttttc caataggata tatcgtttta atggcagttt
    12361 ggttatataa agtttctatt aaaacaggac aattttctat tattcaagat agtattgcta
    12421 gtatttcaga ggaccaaaga atccaactat tattaattgg attttgtttc aacgcatttt
    12481 tagaaggtgc agcaggattt ggtgtgccaa ttgcgatttg tgcagtatta ttaattcaac
    12541 ttggatttga accattaaaa gcagcgatgt tatgtttaat tgctaatggt gcggcgggtg
    12601 cctttggtgc aattggttta ccagttagta ttattgatac gtttaactta agtggaggcg
    12661 ttacaacatt agatgttgcg agatactcag cattaacact tccaatttta aactttatta
    12721 ttccatttgt tttagtattc attgtagatg gtatgaaagg tattaaagaa attttacctg
    12781 tcattttaac agtgagtggt acatatactg gattacaatt attattaaca atattccatg
    12841 gtccagaact agcagacatt attccatcac tagcaacaat ggtggtgtta gcatttgttt
    12901 gtcgtaaatt taaaccgaaa aacattttca gattggaagc gtctgaacat aaaattcaaa
    12961 aacgaacgcc taaagaaatt gtctttgctt ggagtccgtt cgtaatttta actgcctttg
    13021 tattagtatg gagtgcacca ttctttaaaa aattattcca acctggaggt gcgcttgaaa
    13081 gtttagtatt aaaaatgcca attccaaata ctgtgagtga tttatcgcct aaaggaattg
    13141 cgttgcgtct cgatttaatt ggtgcaactg ggacagcgat tttattaaca gtaattatta
    13201 caattttaat tacgaagtta aaatggaaaa gtgcaggtgc tttattggtc gaagcaatta
    13261 aagaattatg gttaccgatc cttacaattt cagctatcct agctattgct aaagttatga
    13321 catacggtgg tttgactgta gcaattggac aaggtattgc taaagctgga gcaattttcc
    13381 cattattctc tccagtatta ggttggattg gtgtgtttat gactggttca gttgtaaata
    13441 acaatacttt attcgcacct attcaagcga cagtggcaca acaaatttca acaagcggtt
    13501 cattacttgt ggcagctaac actgcaggtg gtgtagcagc gaaacttatt tcaccacaat
    13561 caattgccat tgcgactgca gctgttaaaa aagttggtga agaatcagca ttattaaaaa
    13621 tgacgctaaa atacagtatt atatttgttg cctttatttg tatttggacg tttatactaa
    13681 cgttaatatt ctaaatataa ataatgttgt cacttggatt caaatgacat tttaaatcta
    13741 attattcatg aatcgaacta gtacgaaatg cattgagcat cttgtctagt tcgatttttt
    13801 aatgtctaaa aatgtcgtat atgtaattag agtagaaagt gttgaggcgt ttcagaagtt
    13861 gtttagaaaa gtaagtaaaa taaaaaatgc actgagcaac aaaagatgtt gctagtgcat
    13921 ttagatgatt cttatcattt caaataagaa tgtgttaatc aacgtatata agttaaaatt
    13981 ggtttggata aaatgatatc tatcgttgtg tattgtttgt ttttatagtt cgcgacgacg
    14041 tccagctaat aacgctgcac ctaacgctaa tgataatcca ccaaatacag ttgtaccgat
    14101 gaatggattt tcttcaccag tttctggtaa tgcttgagct ttgttagcat ctgcatggtt
    14161 tgctggttgc ttcttatcaa caacaagttc ttgaccaggt ttgattatgt ttttgtcagc
    14221 taatttgtta tctgcagcaa ttttgtcagc agtagtgccg tttgcttttg caatgtcatt
    14281 tactgtatca ccaggtttaa cgacatgtac cccgttgccg tcttctttgc caggtttgtt
    14341 accatcttct ttaccaggtt tgttgccgtc ttctttacca ggtttgttgc catcttcttt
    14401 accaggcttg ttgccgtctt ctttaccagg tttgttgcca tcttctttac caggcttgtt
    14461 gccgtcttct ttgctaggct ttttgttgtc ttctttgcca ggtttgttgc catcttcttt
    14521 accaggcttg ttgccgtctt ctttgctagg ctttttgttg tcttctttgc caggtttgtt
    14581 gccatcttct ttgccaggtt tgttgccgtc ttctttacca ggtttgttgt tgtcttcttt
    14641 gccaggtttg ttgccgtctt ctttgccagg tttgttgccg tcttctttac caggtttttt
    14701 gttgtcttcc tcttttggtg cttgagcatc gtttagcttt ttagcttctg ctaaaatttc
    14761 tttgctcact gaaggatcgt ctttaaggct ttggatgaag ccgttacgtt gttcttcagt
    14821 taagttaggt aaatgtaaaa tttcatagaa agcattttgt tgttctttgt tgaatttgtt
    14881 gtcagctttt ggtgcttgtg catcatttag ctttttagct tctgctaaaa ggttagcgct
    14941 ttggcttggg tcatctttta ggctttggat gaaaccattg cgttgttctt cgtttaagtt
    15001 aggtaaatgt aagatttcat agaaagcatt ttgttgttct ttgttgaatt tgttatccgc
    15061 tttcggtgct tgagattcat ttaacttttt agcttctgac aataggttag cactttgact
    15121 tgggtcatct tttaagcttt ggatgaaacc attgcgttgt tcttcgttca agttaggcat
    15181 gttcaagatt tcatagaaag cattttgttg ttttttgttg aaattgttgt cagctttcgg
    15241 tgcttgagat tcgtttaatt ttttagcttc acctaaaacg ttagtgcttt ggcttggatc
    15301 gtctttaaga ctttgaatga aaccattgcg ttgcgcttcg tttaagttag gcatgttcaa
    15361 aatttcatag aaggcgcttt gttgatcttt gttgaagtta ttttgttgcg catcagcttt
    15421 tggagcttga gagtcattaa gtttttgagc ttcacctaaa acgttagcac tttggcttgg
    15481 atcatcttta aggctttgga taaaaccatt gcgttgatca gcatttaagt taggcatatt
    15541 taagacttga taaaaagcat tttgttgagc ttcatcgtgt tgcgcagcat ttgcagcagg
    15601 tgttacgcca ccagatataa gtaatgtacc taaagttaca gatgcaatac ctacacctag
    15661 tttacgaatt gaataaatgt ttttcttttt caaattaata ccccctgtat gtatttgtaa
    15721 agtcatcata atataacgaa ttatgtattg caatactaaa at
//
LOCUS       DAHHOJ010000061        15740 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.061, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000061 DAHHOJ010000000
VERSION     DAHHOJ010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15740
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.061"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            70..1680
                     /locus_tag="NBH32_001634"
     CDS             70..1680
                     /locus_tag="NBH32_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MutS family DNA mismatch repair protein"
                     /protein_id="HCD1515227.1"
                     /translation="MSTNQTFLIFVIAIILLTSVIGIVGRYMSRQRLLKSMETLWQTI
                     SPLEAFIRPNSHFDYEYKLYKEKFESHSLVDDKTWSDLNMNAIFHKMNYNLTAIGEMK
                     LYACLRGMLSITNKSLLSLFNDNAEFRKNVTYHLALVGKTVYPTFPDQITPVKRQNIL
                     FLCPFLPVISFAVIFINSQVGILLFLMSCLFNIILSATLKRTYEDDLKSIFYASNVLK
                     QGYTISKIKHAPQPEVNFKQFRTARHLTSVLAEVNDEDIGAMVIKLVKLIFMLDYVLF
                     HSIQKSYTTHMNELKNCFDYIAELDNHYALAMYRRTLECYTEPQIDDSNDGIVFSELT
                     HPLIADAVANDFSLSQNILLTGSNASGKSTFMKSIAINIILASAIQTVTASKFVYQPG
                     IVFTSMANADDVLSGDSYFMAELKSIKRIVEIPDNQKIYCFIDEIFKGTNTTERIAAS
                     ESVLSFLHEKSNFRVIAATHDIELAELLKQRYENYHFNEVIENNNIHFDYKIKPGKAN
                     TRNAIELLKITSFPTKIYERAKDNVPKI"
     gene            complement(1782..2807)
                     /gene="tsaD"
                     /locus_tag="NBH32_001635"
     CDS             complement(1782..2807)
                     /gene="tsaD"
                     /locus_tag="NBH32_001635"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCD1515228.1"
                     /translation="MTKDILILAVETSCDETSVSVIKNGRDILSNTVLSQIESHKRFG
                     GVVPEVASRHHVEGITTTINEALVDADVSMEDIDAIAVTEGPGLIGALLIGVNAAKAL
                     AFAYDKPLIPVHHIAGHIYANHIEEPLTFPLIALIVSGGHTELVYMKDHLSFEVIGET
                     RDDAVGEAYDKVARTIGLNYPGGPQVDRLAAEGEDTYSFPRVWLDKDSYDFSFSGLKS
                     AVINQLHNQRQKNIPIIEANVATSFQNSVVEVLTFKAIQACKKYGVQRLIVAGGVASN
                     KGLRQSLADQCKVNDIQLTIPSPKLCTDNAAMIGVAGHYLYQQGRFADLALNGHSNID
                     LEEYSAE"
     gene            complement(2800..3264)
                     /gene="rimI"
                     /locus_tag="NBH32_001636"
     CDS             complement(2800..3264)
                     /gene="rimI"
                     /locus_tag="NBH32_001636"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCD1515229.1"
                     /translation="MDQQSKEQLNIREMTKEDVPQVFDIERRSFNDSSWTIDAFYHEI
                     EQNNFAKYFVLEFEQQIIGYLGLWIVIDQAQITTVAIDDNYRGYGLGQMLLKYGINYA
                     SHTCDVMSLEVRVNNKVAQHVYENLGFQYGGKRKNYYGEGEDAMVMWVNLND"
     gene            complement(3237..3899)
                     /gene="tsaB"
                     /locus_tag="NBH32_001637"
     CDS             complement(3237..3899)
                     /gene="tsaB"
                     /locus_tag="NBH32_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCD1515230.1"
                     /translation="MNSLLIDTSNQPLSVALMQNDEVLAEITTDSKQNHSVQLMPAIS
                     QLFEQSKIAKQQLDAIIVAEGPGSYTGLRIGVTVAKTLAYALDVKLYGVSSLKALAAT
                     IDHTDKLLVPVFDARRQAVYTGIFQWQNGQLVTILEDQYMSIEALRAFLEDLNQPFIY
                     IGKDTVKLQDELQGDVIAQLPNASVMHQLIDKPSDVHTFTPKYHKLAEAERNWINSQK
                     NN"
     gene            complement(3880..4341)
                     /gene="tsaE"
                     /locus_tag="NBH32_001638"
     CDS             complement(3880..4341)
                     /gene="tsaE"
                     /locus_tag="NBH32_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000590797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCD1515231.1"
                     /translation="MIKINNLDEMNQFAMFLVEQLKSGDLILLNGDLGAGKTTLTQFI
                     GKALGVRRTINSPTFNIIKSYRGKNLKLHHMDCYRLEDSDEDLGFDEFFEDQAITVIE
                     WSQFIKDLLPSTHLSINISTISENTRQIELFAQGEHYEQIKEAIIHEFAAH"
     gene            4379..4534
                     /locus_tag="NBH32_001639"
     CDS             4379..4534
                     /locus_tag="NBH32_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515232.1"
                     /translation="MIIHCSIVSNFIFNLYIVTNFNLNFLSLKQIISSKLIVRKFKIF
                     VDILKQI"
     gene            4852..6540
                     /gene="ilvD"
                     /locus_tag="NBH32_001640"
     CDS             4852..6540
                     /gene="ilvD"
                     /locus_tag="NBH32_001640"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCD1515233.1"
                     /translation="MRSDMIKKGDHQAPARSLLHATGALKSPTDMNKPFVAICNSYID
                     IVPGHVHLRELADIAKEAIREAGAIPFEFNTIGVDDGIAMGHIGMRYSLPSREIIADA
                     AETVINAHWFDGVFYIPNCDKITPGMILAAMRTNVPAIFCSGGPMKAGLSAHGKALTL
                     SSMFEAVGAFKEGSISKEEFLDMEQNACPTCGSCAGMFTANSMNCLMEVLGLALPYNG
                     TALAVSDQRREMIRQAAFKLVENIKNDLKPRDIVTREAIDDAFALDMAMGGSTNTVLH
                     TLAIANEAGIDYDLERINAIAKRTPYLSKIAPSSSYSMHDVHEAGGVPAIINELMKKD
                     GTLHPDRITVTGKTLRENNEGKEIKNFDVIHPLDAPYDAQGGLSILFGNIAPKGAVIK
                     VGGVDPSIKTFTGKAICFNSHDEAVEAIDNRTVRAGHVVVIRYEGPKGGPGMPEMLAP
                     TSSIVGRGLGKDVALITDGRFSGATRGIAVGHISPEAASGGPIALIEDGDEITIDLTN
                     RTLNVNQPEDVLARRRESLTPFKAKVKTGYLARYTALVTSANTGGVMQVPENLI"
     gene            6568..8337
                     /gene="ilvB"
                     /locus_tag="NBH32_001641"
     CDS             6568..8337
                     /gene="ilvB"
                     /locus_tag="NBH32_001641"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HCD1515234.1"
                     /translation="MSKTQHEVNQNIDPLKMAESLEPEQLNEKTLNDMRSGSEVLVEA
                     LLKENVDYLFGYPGGAVLPLYDTFYDGKIKHILARHEQGAVHAAEGYARVSGKTGVVV
                     VTSGPGATNVMTGITDAHCDSLPLVVFTGQVATPGIGKDAFQEADILSMTSPITKQNY
                     QVKRVEDIPKIVHEAFHVANSGRKGPVVIDFPKDMGVLATNVDLCDEINIPGYEVVTE
                     PENKDIDTFISLLKEAKKPVVLAGAGINQSKSNQLLTQFVNKHQIPTVTTLLGLGAVP
                     YEDTLFLGMGGMHGSYASNMALTECDLLINLGSRFDDRLASKPDAFAPNAKIVHVDID
                     PSEINKVIHADLGIIADCKRFLECLNDKNVETIAHSDWVKHCQNNKQKHPFKLGEKDP
                     VFCKPQQTIEYIGKITNGEAIVTTDVGQHQMWAAQFYPFKNHGQWVTSGGLGTMGFGI
                     PSSIGAKLANPDKTVVCFVGDGGFQMTNQEMALLPEYGLDVKIVLINNGTLGMVKQWQ
                     DKFFNQRFSHSVFNGQPDFMKMAEAYGVKGFLIDKPEQLEEQLDAAFAYQGPALIEVR
                     ISPTEAVNPMVPSGKSNHEMEGL"
     gene            8337..8591
                     /locus_tag="NBH32_001642"
     CDS             8337..8591
                     /locus_tag="NBH32_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HCD1515235.1"
                     /translation="MTRILKLQVADQVSTLNRITSAFVRLQYNIDTLHVTHSEQPGIS
                     NMEIQVDIQDDTSLHILIKKLKQQINVLTVECYDLVDNEA"
     gene            8729..9733
                     /gene="ilvC"
                     /locus_tag="NBH32_001643"
     CDS             8729..9733
                     /gene="ilvC"
                     /locus_tag="NBH32_001643"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCD1515236.1"
                     /translation="MTTVYYDQDVKTDALQGKKIAVVGYGSQGHAHAQNLKDNGYDVV
                     IGIRPGRSFDKAKEDGFDVFPVAEAVKQADVIMVLLPDEIQGDVYKNEIEPNLEKHNA
                     LAFAHGFNIHFSVIQPPADVDVFLVAPKGPGHLVRRTFVEGSAVPSLFGIQQDASGQA
                     RNIALSYAKGIGATRAGVIETTFKEETETDLFGEQAVLCGGVSKLIQSGFETLVEAGY
                     QPELAYFEVLHEMKLIVDLMYEGGMENVRYSISNTAEFGDYVSGPRVITPDVKENMKA
                     VLTDIQNGNFSNRFIEDNKNGFKEFYKLREEQHGHQIEKVGRELREMMPFIKSKSIEK
                     "
     gene            9763..11292
                     /locus_tag="NBH32_001644"
     CDS             9763..11292
                     /locus_tag="NBH32_001644"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD1515237.1"
                     /translation="MSSHIQIFDTTLRDGEQTPGVNFTFDERLRIALQLEKWGVDVIE
                     AGFPASSTGSFKSVQAIAQTLTTTAVCGLARCKKSDIDAVYEATKDAAKPVVHVFIAT
                     SPIHLEHKLKMSQEDVLASIKEHVTYAKQLFDVVQFSPEDATRTELPFLVKCVQTAVD
                     AGATVINIPDTVGYSYHDEYAHIFKTLTESVTSSNEIIYSAHCHDDLGMAVSNSLAAI
                     EGGARRIEGTVNGIGERAGNAALEEVALALYVRNDYYGAQTALNLEETKKTSDLISRY
                     AGIRVPRNKAIVGQNAFSHESGIHQDGVLKHRETYEIMTPQLVGVSTTELPLGKLSGK
                     HAFSEKLKALGYDIDKEAQIDLFKQFKAIADKKKSVSDRDIHAIIQGSEHEHQALYKL
                     ETLQLQYVSSGLQSAVVVVKDKEDHIYQDSSIGTGSIVAIYNAVDRIFQKETELIDYR
                     INSVTEGTDAQAEVHVNLLIEGKTVNGFGIDHDILQASCKAYVEAHAKFAAENVEKVG
                     N"
     gene            11295..12341
                     /gene="leuB"
                     /locus_tag="NBH32_001645"
     CDS             11295..12341
                     /gene="leuB"
                     /locus_tag="NBH32_001645"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCD1515238.1"
                     /translation="MTYNIVALPGDGIGPEILNGSLSLLEIISNKYNFNYQIEHHEFG
                     GASIDTFGEPLTEKTLNACKRADAILLGAIGGPKWTDPNNRPEQGLLKLRKSLNLFAN
                     IRPTIVVKGASSLSPLKEDRVEGTDLVIVRELTSGIYFGEPRHFNNHEALDSLTYTRE
                     EIERIVHVAFKLAASRRGKLTSVDKENVLASSKLWRKVVNEVSQLYPEVTVNHLLVDA
                     CSMHLITNPKQFDVIVCENLFGDILSDEASVIPGSLGLSPSASFSNDGPRLYEPIHGS
                     APDIAGKNVANPFGMILSLAMCLRESLNQPDAADELEQHIYNMIEHGQTTADLGGKLN
                     TTDIFEILSQKLNH"
     gene            12355..13725
                     /gene="leuC"
                     /locus_tag="NBH32_001646"
     CDS             12355..13725
                     /gene="leuC"
                     /locus_tag="NBH32_001646"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCD1515239.1"
                     /translation="MGQTLFDKVWNRHVLYGKLGEPQLLYIDLHLIHEVTSPQAFEGL
                     RLQNRKLRRPDLTFATLDHNVPTIDIFNIKDEIANKQITTLQKNAIDFGVHIFDMGSD
                     EQGIVHMVGPETGLTQPGKTIVCGDSHTATHGAFGAIAFGIGTSEVEHVFATQTLWQT
                     KPKNLKIDINGTLPTGVYAKDIILHLIKTYGVDFGTGYALEFTGETIKNLSMDGRMTI
                     CNMAIEGGAKYGIIQPDDITFEYVKGRPFADNFAKSVDKWRELYSDDDAIFDRVIELD
                     VSTLEPQVTWGTNPEMGVNFSEPFPEINDINDQRAYDYMGLEPGQKAEDIDLGYVFLG
                     SCTNARLSDLIEASHIVKGNKVHPNITAIVVPGSRTVKKEAEKLGLDTIFKNAGFEWR
                     EPGCSMCLGMNPDQVPEGVHCASTSNRNFEGRQGKGARTHLVSPAMAAAAAIHGKFVD
                     VRKVVV"
     gene            13726..14298
                     /gene="leuD"
                     /locus_tag="NBH32_001647"
     CDS             13726..14298
                     /gene="leuD"
                     /locus_tag="NBH32_001647"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCD1515240.1"
                     /translation="MAAIKPITTYKGKIVPLFNDNIDTDQIIPKVHLKRISKSGFGPF
                     AFDEWRYLPDGSDNPDFNPNKPQYKGASILITGDNFGCGSSREHAAWALKDYGFHIII
                     AGSFSDIFYMNCTKNAMLPIVLEKNAREHLAKYVEIEVDLPNQTVSSPDKSFHFEIDE
                     TWKNKLVNGLDDIAITLQYESLIEKYEKSL"
     gene            14313..15581
                     /gene="ilvA"
                     /locus_tag="NBH32_001648"
     CDS             14313..15581
                     /gene="ilvA"
                     /locus_tag="NBH32_001648"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase IlvA"
                     /protein_id="HCD1515241.1"
                     /translation="MTVKTTVSTKDIDEAFLRLKDIVKETPLQLDHYLSQKYDCKVYL
                     KREDLQWVRSFKLRGAYNAISVLSDEAKSKGITCASAGNHAQGVAYTAKKLNLNAVIF
                     MPVTTPLQKVNQVKFFGNSNVEVVLTGDTFDHCLAEALTYTSEHQMNFIDPFNNVHTI
                     SGQGTLAKEMLEQAKSDNVNFDYLFAAIGGGGLISGISTYFKSYSPNTKIIGVEPSGA
                     SSMYESVVVNNQVITLPNIDKFVDGASVARVGDITFEIAKKNVDNYVQVDEGAVCSTI
                     LDMYSKQAIVAEPAGALSVSALENYKDHIKGKTVVCVISGGNNDINRMKEIEERSLLY
                     EEMKHYFILNFPQRPGALREFVNDVLGPQDDITKFEYLKKSSQNTGTVIIGIQLKDDD
                     DLIQLKQRVNHFDPSNIYINENKMLYSLLI"
BASE COUNT         5320 a         2679 c         2785 g         4956 t
ORIGIN      
        1 tgacagtatt cgaatgatgt ggttagctca agatcaaaca tattattcaa aataaataag
       61 gagatactta tgagcacaaa tcaaacattt ttaatatttg ttatagcaat tattctactt
      121 acatctgtaa taggaattgt tggacgatac atgagtcgtc aacgtctatt aaaatctatg
      181 gaaacattat ggcaaacgat ttctccatta gaagctttta tcagaccgaa ctcacatttc
      241 gactatgagt ataagctcta caaggaaaaa tttgaatcac attcattagt tgatgataaa
      301 acttggtccg acttaaatat gaatgcaatc tttcataaga tgaattataa tttaacagct
      361 attggtgaaa tgaagctata tgcctgttta cgtggaatgc tttcaattac gaacaaatca
      421 ttacttagtt tatttaatga taatgctgaa tttagaaaaa acgtaacata tcatttagct
      481 ttggttggta aaactgttta tccaacattt ccagaccaaa tcacaccggt aaaacgtcaa
      541 aatatattgt ttctatgccc atttttacca gttatctcat ttgcagttat atttattaat
      601 tcacaagttg gtattttatt atttcttatg agttgcctat ttaatatcat tttatctgca
      661 actttaaagc gtacatatga ggatgactta aagtctatct tttatgcatc caatgtttta
      721 aaacaaggct atactatctc aaaaattaaa catgctccac aacccgaagt caattttaag
      781 caatttagaa cagcacgtca tttaacgagt gttttagctg aagttaatga tgaagatatt
      841 ggagctatgg taattaaact agttaaactt attttcatgc ttgattatgt tttattccat
      901 tcaattcaaa aaagctatac aacacatatg aatgagctaa aaaattgttt cgattacata
      961 gctgaattag ataatcatta tgcattagca atgtatcgta gaacgctaga atgctatact
     1021 gaaccacaaa ttgatgattc aaatgatggc atagtattct ctgagttaac acatccactc
     1081 atagcagatg cagtagccaa tgatttctca ctatcacaaa atatactgtt aactgggtct
     1141 aatgcttcgg gtaaatcaac atttatgaaa tccatagcaa taaacattat tcttgcatcg
     1201 gctatacaaa cagttaccgc aagcaaattt gtatatcaac ctggaattgt gttcacgtca
     1261 atggcaaatg ccgatgatgt gttatcaggc gatagttatt ttatggctga attaaaatct
     1321 attaagcgta ttgttgaaat tccagacaat caaaaaatat actgctttat agatgaaatt
     1381 tttaaaggta ccaacacaac tgaacgaatt gccgcttcag aatcagtact atcattttta
     1441 catgaaaaat ctaactttag agttattgca gcaacacatg atattgagtt agctgaactc
     1501 ttaaaacaac gttatgaaaa ttatcatttc aatgaggtaa tagaaaataa taacatacat
     1561 tttgattaca aaattaagcc tggcaaagca aatacacgta atgccatcga attattaaaa
     1621 atcacttcat ttccaacaaa aatatatgaa cgagcaaaag ataatgtccc gaaaatttag
     1681 catttaactt taaacataaa aacgtcagct atcacatgac agaagactat gaacagtttc
     1741 aataatgttc atcgtaatca tgttaataac tgacgtttat tttattctgc agaatactct
     1801 tctaaatcta tattgctgtg cccatttaat gctaaatcag caaatcgacc ttgctgatac
     1861 aaatagtggc cggcaacgcc tatcattgca gcattatctg tgcataattt aggacttggg
     1921 atagttaatt gaatgtcatt gactttgcat tgatccgcta aagattgacg taatccttta
     1981 ttactcgcca cgccaccagc aacaattaat cgctgaacac catatttttt acaagcttga
     2041 atagctttaa acgtaagcac ctctacaaca ctgttttgaa agctcgttgc tacgttagct
     2101 tcaatgattg gaatattttt ttgtcgttga ttgtgaagtt gattgattac ggcacttttc
     2161 aacccactaa aactaaaatc ataactatct ttatccaacc aaacacgagg gaatgaataa
     2221 gtatcttcac cttcagcagc caaccgatca acttgtggac cacctggata atttaaacca
     2281 attgttcgtg ccactttatc ataagcttca cctactgcgt catctcgtgt ttcaccaatg
     2341 acttcaaatg ataaatgatc tttcatataa actaattcag tatgtccacc tgaaacaata
     2401 agtgcaatta gcgggaatgt taatggctct tctatgtgat tagcatatat atgtcctgca
     2461 atatgatgaa caggaataag tggcttatcg taagcaaatg ccaatgcttt ggctgcatta
     2521 acacctatta gtaacgcacc aattagtcca gggccttctg taaccgctat ggcatcaata
     2581 tcttccattg atacatcggc atccactaga gcctcgttta ttgttgttgt tataccttca
     2641 acgtgatgtc tacttgccac ttcgggaacg acaccgccaa atcgtttatg actttcaatc
     2701 tgacttaaaa ctgtatttga taaaatatct ctgccatttt ttataacact aacgcttgtt
     2761 tcatcacaac ttgtttcaac agctagtatt aatatatctt tagtcattta aattcaccca
     2821 cataaccatt gcgtcctcac cttcaccata ataattttta cgtttaccac catattgaaa
     2881 tcctaaattt tcatatacat gttgtgccac tttattatta actcttactt ctaaactcat
     2941 cacatcacaa gtgtgacttg catagtttat tccgtatttt aaaagcattt gacctaaacc
     3001 atagcctcta taattatcat cgattgcaac tgtcgtaatt tgagcttgat cgataacaat
     3061 ccataaacct aaataaccaa taatttgttg ttcaaattct aagacaaaat atttcgcaaa
     3121 gttattttgt tctatttcat gataaaatgc gtcaattgtc caagaactat cattgaaact
     3181 ccgacgctca atatcaaaga cttgtggcac atcttctttg gtcatctctc taatgtttaa
     3241 ttgttctttt gactgttgat ccaatttcgt tccgcctcag ctaatttatg gtatttagga
     3301 gtaaatgtat gtacgtctga aggtttatct atcaattgat gcatgactga tgcatttggt
     3361 agctgcgcaa tcacatcacc ttgtaattca tcttgtaatt ttacagtatc tttcccaata
     3421 taaataaatg gttggtttaa atcttctaaa aaagctcgca atgcctctat cgacatatat
     3481 tgatcttcta aaatagtcac taattgacca ttttgccact ggaatatgcc tgtataaact
     3541 gcttgtcgtc ttgcatcaaa cacaggaacc aataatttat cagtatgatc gattgttgct
     3601 gccaatgcct ttaatgatga aacaccatat aatttaacat ctaacgcata cgctaatgtt
     3661 ttagcaacag taacaccgat acgtaagcca gtatatgaac caggaccttc agcaacaata
     3721 atcgcatcta attgctgttt agctattttg ctctgctcaa atagctgact aatcgcaggc
     3781 attaattgca ctgaatgatt ttgtttagaa tccgtagtta tttcagctaa aacttcatcg
     3841 ttttgcatca atgctactga taatggttga ttcgatgtat caatgagcag cgaattcatg
     3901 gataattgcc tccttaattt gttcataatg ttctccttgc gcgaacaact caatttgtct
     3961 tgtattttca gatattgttg aaatattaat agataaatgc gtcgatggaa gtaaatcttt
     4021 tataaattga ctccattcaa taacagtaat tgcctgatct tcgaaaaatt catcaaaccc
     4081 taaatcttca tcagaatctt ctaagcgata acaatccata tgatgcaatt ttaaattttt
     4141 acccctatat gatttaatga tgttaaatgt cggggaatta atcgttcgtc ttacaccaag
     4201 agcttttcct ataaattgcg ttaacgttgt tttacctgct cctaaatctc cgttaagtaa
     4261 aatcaaatca ccacttttca attgctcaac taaaaacata gcaaattgat tcatttcatc
     4321 taaattattt atctttatca atgttgattc tcctatattg tgcttttcat tcataaaaat
     4381 gattatccat tgttcaatcg tatctaactt tatatttaac ctttatattg taacaaattt
     4441 caacttaaat ttcttatctt tgaaacagat tatctcttca aagttaattg taagaaaatt
     4501 taaaatattt gttgacatac taaagcagat atagtaaatt aaatttatca aatttttaga
     4561 caattctaac tattaaagtg atatatacca ttcacggaag gagtataata aaatgcttaa
     4621 tcaatatact gaacatcaac cgacaacttc aaatattatt attttattat actctttagg
     4681 actcgaacgt tagtaaatat ttactaaacg ctttaagtcc tatttctgtt tgaatgggac
     4741 ttgtaaacgt ccccataata ttgggacgtt tttttatgtt ttatctttca attacttatt
     4801 tttattacta taaaacatga ttaatcatta aaatttacgg gggaatttac tatgcgaagc
     4861 gacatgatca aaaaaggaga tcaccaagca ccagcaagaa gtcttttaca tgccacgggc
     4921 gcgctaaaaa gtccaactga tatgaacaaa ccatttgtag ctatttgtaa ctcttatatt
     4981 gatattgttc ctggacatgt tcatttaaga gagcttgcag atatagctaa agaagcaatt
     5041 agagaagccg gtgccattcc atttgaattc aatacaattg gtgttgatga tggaatagct
     5101 atgggacata tcggaatgcg atattctcta ccatcacgtg aaattattgc agatgcagct
     5161 gaaactgtaa ttaacgctca ttggtttgac ggcgtatttt acattcctaa ttgtgacaag
     5221 attacacccg gtatgatttt agcagccatg aggacaaacg taccagctat cttttgctct
     5281 ggtggaccaa tgaaagctgg cttatctgca catggaaaag cattaacact ttcatcaatg
     5341 tttgaagcag tcggcgcatt taaagaagga tcgatttcta aagaagaatt tttagatatg
     5401 gaacaaaatg cctgccctac ttgtggttca tgtgctggga tgtttactgc aaattcaatg
     5461 aactgtttga tggaagtttt aggtctagca ttaccataca acggtactgc acttgcagtc
     5521 agtgatcaac gacgcgaaat gattcgccaa gcagctttta aattagttga aaatattaaa
     5581 aatgatttaa aaccacgtga tatcgttact cgcgaagcaa ttgatgatgc atttgcactt
     5641 gatatggcta tgggtggttc aacaaacaca gtactgcata cgttagccat tgccaatgaa
     5701 gctggtattg attatgactt agagcgcatt aatgctattg ccaaacgcac gccatattta
     5761 tcaaaaatag cacctagttc atcgtattca atgcatgatg tgcatgaagc tggtggcgtc
     5821 ccagcaatta ttaatgaatt gatgaagaaa gatggcacgt tacacccaga tagaatcaca
     5881 gttactggca aaacgttacg tgaaaataac gaaggcaaag aaattaagaa ctttgatgtc
     5941 attcaccctc ttgatgcacc atatgatgca caaggcggtt tatctatctt atttggtaat
     6001 atcgccccta aaggcgcagt tattaaagtt ggcggcgttg atccatctat caaaacattt
     6061 actgggaaag caatttgttt caattcgcat gatgaagctg ttgaagcaat agacaatcgt
     6121 accgttcgtg caggccacgt cgttgtcatt agatatgaag gacctaaagg tggaccaggt
     6181 atgcctgaaa tgttagcacc tacttcctct attgttggtc gcggcttagg taaagatgtt
     6241 gcattaatta ctgatgggcg tttttccggt gccacaagag gtattgcagt tggtcatatt
     6301 tcccctgaag ctgcatctgg tggaccaatt gccttaattg aagatggaga tgagattact
     6361 attgatttaa caaatcgtac attaaacgta aaccagcctg aagatgttct agcgcgtcgc
     6421 cgagaatctt taacaccatt taaagcgaaa gtaaaaacag gttatctagc tcgttatact
     6481 gccctagtaa ctagcgcaaa tacaggtggc gtcatgcaag tccctgagaa tttaatttaa
     6541 tttattttta tattggagat ggttaaaatg tctaaaactc aacatgaagt aaaccaaaat
     6601 attgaccctt taaaaatggc tgaatcactt gaacccgaac aactaaatga aaaaacttta
     6661 aatgatatgc gttcaggatc agaagtgcta gtagaagctc tacttaaaga aaatgtggat
     6721 tatttattcg gttatcctgg tggtgccgta ctacctttat atgacacgtt ttatgatggt
     6781 aaaatcaaac atattttagc aagacacgaa caaggtgctg ttcatgctgc agaaggttat
     6841 gcacgtgtat ctggtaaaac tggcgtcgtt gtagttacaa gcggtccagg tgcaacaaat
     6901 gtaatgacag gtattacgga tgcacattgc gactctttac ctctagttgt attcaccgga
     6961 caagttgcta caccaggcat tggtaaagat gcattccaag aagcggatat tctatctatg
     7021 acttcaccaa ttacaaaaca aaattatcaa gtgaaacgtg ttgaagatat ccctaaaatc
     7081 gtacacgaag ctttccatgt agcgaattct ggacgcaaag gtcctgtagt gattgatttt
     7141 ccaaaagata tgggtgtttt agctacaaat gtggatttat gcgacgaaat caatattcca
     7201 ggttatgaag ttgttacaga accagaaaat aaagacattg acactttcat ctcactttta
     7261 aaagaagcga aaaagcctgt cgtattagcc ggcgcaggta ttaatcaatc aaaatcaaat
     7321 caattattaa cacagtttgt taataaacat caaatcccaa cagttactac tttactaggt
     7381 ctaggtgctg taccttatga ggatacacta tttttaggta tgggaggtat gcatggttct
     7441 tatgctagta acatggcatt aactgagtgt gatttactca ttaatttagg tagccgcttc
     7501 gatgatagat tagcaagcaa acctgatgcc tttgcaccta acgccaaaat tgtacatgta
     7561 gatattgatc cttcagaaat caataaagtt attcatgcag atttaggtat tattgcagac
     7621 tgtaaaagat ttttagaatg tttaaatgat aaaaatgttg agactatagc acacagtgac
     7681 tgggttaaac attgtcaaaa taataagcag aaacacccat ttaaacttgg tgaaaaagat
     7741 ccagtatttt gtaagccaca acaaacaatc gaatatatcg gcaaaattac aaatggtgaa
     7801 gcaattgtta ctacagacgt gggacaacat caaatgtggg cagctcaatt ttatccattt
     7861 aaaaatcacg gacaatgggt tacaagtggt ggtttaggaa caatgggatt cggtattcct
     7921 tcgtcaattg gtgccaaatt agctaatcct gataaaacag tcgtatgttt cgtcggtgac
     7981 ggtggtttcc aaatgacaaa ccaagaaatg gcactattac ccgaatatgg tttagatgtc
     8041 aaaatcgtac taatcaataa tggaacacta ggtatggtta aacaatggca agataagttc
     8101 tttaatcaac gcttctcaca ctcagtattt aatggtcaac ctgattttat gaaaatggca
     8161 gaagcatatg gcgtcaaagg tttcttaatc gataagccag aacaactgga agaacaatta
     8221 gatgcagcgt ttgcttatca aggaccagct ttaattgagg ttcgtatttc ccctactgaa
     8281 gctgtaaacc caatggttcc gagtggcaaa tcaaatcatg aaatggaggg cttataatga
     8341 caagaattct taaattacaa gttgcggatc aagtcagcac gctaaatcga attacaagtg
     8401 cttttgttcg cctacaatat aatatcgata cattacatgt tacacattct gaacaacctg
     8461 ggatttctaa catggaaatt caagtcgata ttcaagatga tacatcactt catatattaa
     8521 ttaaaaaatt aaaacaacaa attaatgttt taacggttga atgctacgac cttgttgata
     8581 acgaagctta attttaagac aaaggcaatg atgcgctaat tagttataga tatatcatag
     8641 gctgctagtt aacatctgcc actattacaa agttatattt cagaattttc aaaaaaacaa
     8701 aatatttaat tatttggagg aatttattat gacaacagtt tattatgatc aagatgtaaa
     8761 aacggacgct ttacaaggca aaaaaattgc agtagtaggt tatggatcac aaggccacgc
     8821 gcatgcacaa aacttaaaag acaatggata tgatgtagtc atcggtattc gcccaggtcg
     8881 ttcttttgac aaagctaaag aagatggatt tgatgtgttc cctgttgcag aagcagttaa
     8941 gcaagctgat gtaattatgg tgctattacc tgatgaaatt caaggtgatg tatacaaaaa
     9001 cgaaattgaa ccaaatttag aaaaacataa tgcgcttgca tttgctcatg gctttaacat
     9061 tcattttagt gttattcaac caccagctga tgttgatgta tttttagtag ctcctaaagg
     9121 accgggtcat ttagttagac gtacatttgt tgaaggttct gctgtaccat cactatttgg
     9181 tattcaacaa gacgcttcag gtcaagcacg taatattgct ttaagttatg caaaaggtat
     9241 tggtgcaact cgtgcaggtg ttattgaaac aacatttaaa gaagaaactg agacagattt
     9301 atttggtgaa caagcagtac tttgcggtgg tgtatcgaaa ttaattcaaa gtggctttga
     9361 aacattagta gaagcgggtt atcaaccaga attagcttat tttgaagtat tacatgaaat
     9421 gaaattaatc gttgatttga tgtatgaagg cggtatggaa aatgtacgtt actcaatttc
     9481 aaatactgct gaatttggtg actatgtttc aggaccacgt gttatcacac cagatgttaa
     9541 agaaaatatg aaagctgtat taactgatat ccaaaatggt aacttcagta atcgctttat
     9601 cgaagacaat aaaaatggat tcaaagaatt ttataaatta cgcgaagaac aacatggtca
     9661 tcaaattgaa aaagttggtc gtgaattacg cgaaatgatg ccttttatta aatctaaaag
     9721 cattgaaaaa taagatagac ctacaatgag gagttgttaa atatgagtag tcatattcaa
     9781 atttttgata cgacactaag agacggtgaa caaacaccag gagtgaattt tacttttgat
     9841 gaacgcttgc gtattgcatt gcaattagaa aaatggggtg tagatgttat tgaagctgga
     9901 tttcctgctt caagtacagg tagctttaaa tctgttcaag caattgcaca aacattaaca
     9961 acaacggctg tatgtggttt agctagatgt aaaaaatctg acatcgatgc tgtatatgaa
    10021 gcaacaaaag atgcagcgaa gccggtcgtg catgttttta tagcaacatc acctattcat
    10081 cttgaacata aacttaaaat gtctcaagaa gacgttttag catctattaa agaacatgtc
    10141 acatacgcga aacaattatt tgacgttgtt caattttcac ctgaagatgc aacgcgtact
    10201 gaattaccat tcttagtgaa atgtgtacaa actgccgttg acgctggagc tacagttatt
    10261 aatattcctg atacagtcgg ctacagttac catgatgaat atgcacatat tttcaaaacc
    10321 ttaacagaat ctgtaacatc ttcaaatgaa attatttata gtgctcattg ccatgacgat
    10381 ttaggaatgg ctgtttcaaa tagtttagct gcaattgaag gcggtgcaag acgaattgaa
    10441 ggcactgtaa atggtattgg tgaacgagca ggtaatgcag cacttgaaga agtcgcgctt
    10501 gcactatacg ttcgaaatga ttattatggt gctcaaactg cccttaatct cgaagaaact
    10561 aaaaaaacat cggatttaat ttcaagatat gcaggtattc gagtgcctag aaataaagca
    10621 attgttggcc aaaatgcatt tagtcatgaa tcaggtattc accaagatgg cgtattaaaa
    10681 catcgtgaaa catatgaaat tatgacacct caacttgttg gtgtaagcac gactgaactt
    10741 ccattaggaa aattatctgg taaacacgcc ttctcagaga agttaaaagc attaggttat
    10801 gacattgata aagaagcgca aatagattta tttaaacaat tcaaggccat tgcggacaaa
    10861 aagaaatctg tttcagatag agatattcat gcgattattc aaggttctga gcatgagcat
    10921 caagcacttt ataaattgga aacactgcaa ctacaatatg tctctagcgg ccttcaaagt
    10981 gctgttgttg ttgttaaaga taaagaggat catatttacc aggattcaag tattggtact
    11041 ggttcaatcg tagcaattta caatgcagtt gatcgtattt tccaaaaaga gacagaatta
    11101 attgattatc gtattaattc tgtcactgaa ggtactgatg cccaagcaga agtacatgta
    11161 aatttattga ttgaaggtaa gactgtcaat ggctttggta ttgatcatga tattttacaa
    11221 gcctcttgta aagcatacgt agaagcacat gctaaatttg cagctgaaaa tgttgaaaag
    11281 gtaggtaatt aattatgact tataacattg ttgccctacc tggtgatgga atcggtccag
    11341 aaattttgaa cggatctcta tccttgcttg aaattataag taataaatat aactttaatt
    11401 atcaaataga gcaccacgaa tttggtggtg cctctattga tacattcggc gagcctttaa
    11461 ctgagaaaac cttaaatgcg tgtaaaagag cagatgctat tttactgggt gcaatcggtg
    11521 gacctaaatg gacagatcct aacaatcgac cagaacaagg attattaaaa ttacgtaaat
    11581 cattaaattt atttgcaaat atacgcccca ctatcgttgt caaaggcgct agttctttat
    11641 cacctttaaa ggaagatcgc gttgaaggca cagatttagt tatagtccgt gaattgacaa
    11701 gtggtattta ttttggagaa cctagacatt ttaataatca cgaggcctta gattctctta
    11761 cttatacaag agaagaaata gaacgcattg ttcacgtagc atttaaattg gctgcttcaa
    11821 gacgaggaaa actaacatca gttgataaag aaaatgtatt agcttctagt aaattgtggc
    11881 gcaaagtcgt aaatgaagtg agtcaattat atccagaagt aacagtaaat cacttacttg
    11941 ttgatgcttg tagcatgcat ttaatcacaa atccaaaaca atttgacgtc atagtttgtg
    12001 aaaacttatt tggcgatatt ttaagtgatg aagcttcagt gattcctggt tcacttggtt
    12061 tatcaccttc tgctagtttt agtaacgatg gtccaagatt atatgagcct attcatggat
    12121 cagcaccaga tattgcaggt aaaaacgttg ccaatccatt tggaatgatt ctatctttag
    12181 cgatgtgttt acgtgaaagc ttaaatcaac cagatgctgc agatgaatta gaacaacata
    12241 tttacaacat gattgaacat gggcaaacga cagcagattt aggcggcaaa ctgaatacta
    12301 ctgatatttt cgaaattcta tctcaaaaat tgaatcacta agggggagat gtaaatgggt
    12361 caaacattat ttgacaaggt gtggaacaga catgtgttat acgggaaatt gggcgaaccg
    12421 caactattat acattgattt acaccttata catgaagtta cttctcctca agcatttgaa
    12481 ggacttaggc ttcaaaacag aaaattaaga cgcccagatt taacatttgc aacactcgat
    12541 cacaatgttc ctactattga tattttcaat attaaagatg aaattgcaaa caaacaaatc
    12601 acaacattac aaaaaaacgc catagatttt ggggtgcata tttttgatat gggctctgat
    12661 gaacaaggta ttgttcacat ggtaggacct gagacaggac ttacacagcc cggcaaaaca
    12721 atcgtttgtg gtgactctca cacagcaaca catggagcat ttggtgctat tgcatttgga
    12781 attggaacaa gtgaagttga acatgttttc gcaactcaaa cgctatggca aacaaaaccc
    12841 aaaaacttaa aaatcgatat taatggtacc ttaccaacag gcgtctatgc taaggacatt
    12901 attctgcatt taattaaaac gtatggtgtt gactttggta caggctatgc tttggaattt
    12961 actggcgaaa caattaaaaa cctttcaatg gacggtcgaa tgactatttg taacatggct
    13021 atcgaaggtg gtgccaaata cggcataatc caacctgatg atataacatt tgaatatgtt
    13081 aaagggagac catttgccga taacttcgct aaatcagttg ataagtggcg tgagctatat
    13141 tctgatgacg acgcgatatt tgatcgtgta attgaacttg atgtttcaac attagaacca
    13201 caagtgacat ggggaactaa tcctgaaatg ggtgttaatt tcagtgaacc attccctgaa
    13261 atcaatgata tcaacgatca acgtgcgtat gattatatgg gattagaacc aggtcaaaaa
    13321 gctgaagata tcgacttagg gtatgttttc ctcggttcat gtacaaatgc tagactatca
    13381 gatttgattg aagctagtca tattgttaaa ggaaataaag ttcacccaaa tattacagct
    13441 attgtcgtac caggttctcg tacagtaaaa aaagaagctg aaaaattagg tctagatact
    13501 atctttaaaa atgcaggatt tgaatggcgt gaaccaggat gttcaatgtg tttaggcatg
    13561 aatcctgacc aagtacctga gggtgtacat tgtgcatcta caagtaatcg aaacttcgaa
    13621 ggacgacaag gcaaaggtgc tagaacacat ttagtatccc ctgctatggc agcagcagca
    13681 gctattcatg gtaaatttgt ggacgtaaga aaggtggttg tttaaatggc agcaatcaaa
    13741 cctattacaa catataaagg taaaatagtc cctcttttca acgataatat cgatacagat
    13801 caaatcattc ctaaagtaca cttaaagcgt atttcaaaaa gtggctttgg tccatttgct
    13861 tttgatgaat ggcggtactt acctgatggt tcagataatc ctgatttcaa tccaaataag
    13921 cctcaatata aaggtgcatc catattaatt actggagata attttggatg tggttcaagt
    13981 cgtgaacatg cagcttgggc acttaaggac tatggctttc atattattat tgcaggaagt
    14041 ttcagtgaca ttttttatat gaattgtaca aaaaatgcaa tgttaccaat tgttttagaa
    14101 aaaaatgcac gtgaacatct tgctaaatat gttgaaattg aggtcgattt accaaatcaa
    14161 actgtgtcat caccagacaa gagtttccat tttgaaattg atgaaacttg gaagaataaa
    14221 cttgtaaatg gcttagatga cattgcaatc accctacaat atgaatcatt aatagaaaaa
    14281 tatgaaaaat cactttaagg gagttgaata ttatgacagt caaaacaaca gtttctacga
    14341 aggatatcga tgaagcattt ttaagactta aagatattgt caaagaaaca cctttacaat
    14401 tagaccatta cttatctcaa aagtatgatt gtaaagttta tttaaaacga gaagatttac
    14461 aatgggtacg ttcttttaaa ttaagaggtg cttacaacgc tatttctgtt ttatcagatg
    14521 aagctaaaag taaaggaatt acatgtgcga gtgcaggtaa tcatgctcaa ggtgttgcct
    14581 atacagctaa aaaacttaat ttaaacgctg ttatctttat gccagtcact acacctttac
    14641 aaaaggtaaa tcaagtaaag ttctttggaa atagtaacgt tgaagttgta ctcactggtg
    14701 atacatttga tcactgttta gctgaagctt taacttatac aagtgaacat caaatgaact
    14761 ttatagatcc attcaataat gttcatacaa tttctggaca aggtacgctt gctaaagaaa
    14821 tgctagaaca agcaaagtct gacaatgtta actttgatta tctatttgcc gcaattggtg
    14881 gtggtggttt aatttcaggt attagtacct attttaaatc ctattctccc aacacaaaaa
    14941 ttatcggagt tgaaccttca ggtgctagta gtatgtatga atctgttgtc gtaaataatc
    15001 aggttatcac attacctaat atcgataaat ttgtcgatgg agcttctgta gccagagttg
    15061 gcgatattac ttttgaaatt gccaaaaaaa atgtagataa ctacgtacaa gtggatgaag
    15121 gtgctgtatg ttcaacaatt ttggatatgt attcaaaaca agcaatagta gcagaacccg
    15181 ctggcgcttt aagtgtaagt gcgcttgaaa actataaaga tcatattaaa ggtaaaacag
    15241 tggtttgtgt cattagtggt ggtaataatg atattaatcg aatgaaagaa attgaagaac
    15301 gttcattact atacgaagaa atgaagcatt actttatctt aaatttccct caacgtccag
    15361 gtgcattgag agaatttgta aatgacgtat taggacctca agacgatatt actaaatttg
    15421 aatacttaaa aaaatcttct caaaatacag gtactgtcat tattggtatt caacttaaag
    15481 atgatgatga tttaatacaa ctcaaacaac gtgtaaatca tttcgatcct tccaatattt
    15541 atattaatga aaataagatg ttatattcat tgttaattta gcacatagta agaaaagcag
    15601 tcataaattg atttctgatt gaaatcatct tatgactgct ttttattata ctttacattt
    15661 ctcgttcgtc gctaagaacc tttcttgact ggaaatgata atttaataat gtacactttt
    15721 gattgtttaa acatgtacaa
//
LOCUS       DAHHOJ010000062        15590 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.062, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000062 DAHHOJ010000000
VERSION     DAHHOJ010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15590)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15590)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15590
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.062"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            68..562
                     /gene="tpx"
                     /locus_tag="NBH32_001649"
     CDS             68..562
                     /gene="tpx"
                     /locus_tag="NBH32_001649"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCD1515242.1"
                     /translation="MTEITFKGGPIHLKGQQINEGDFAPDFTVLDNDLNQVTLADYAG
                     KKKLISVVPSIDTGVCDQQTRKFNSEASKEEGIVLTISADLPFAQKRWCASAGLDNVI
                     TLSDHRDLSFGENYGVVMEELRLLARAVFVLDADNKVVYKEIVSEGTDFPDFDAALAA
                     CKII"
     gene            685..1632
                     /locus_tag="NBH32_001650"
     CDS             685..1632
                     /locus_tag="NBH32_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1515243.1"
                     /translation="MAEQQTIMERLFHTLDEKAKTLNNENGQSFIENLGLAMEQVYTN
                     ERGLLEQSTLQDRRKAFQFAYLSLMQEEKIQANHQITPDSIGLILGFLVERFMNNQEE
                     LHIVDIASGAGHLSATVKEVLPEIAVMHHLIEVDPVLSRVSVHLANFLEIPFDVYPQD
                     AIMPLPLEEADIVIGDFPVGYYPIDERSKEFKLGFEEGHSYSHYLLIEQAINALKDAG
                     YAFLVVPSNIFTGEHVKQLEKYIATETEMQAFLNLPPTLFKNEKARKSILILQKKKSG
                     ETKPVEVLLANIPDFKNPSQFQGFMTELNQWMDTNRPKK"
     gene            1720..2922
                     /locus_tag="NBH32_001651"
     CDS             1720..2922
                     /locus_tag="NBH32_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCD1515244.1"
                     /translation="MSKLILAINAGSSSLKFQLIRMPEEELVTKGLIERIGLKDSIFT
                     IEVNGEKVKTVQDIKDHVEAVDIMLDAFKAHNIINDINDIDGTGHRVVHGGEKFPESV
                     AITDEVEKEIEELSELAPLHNPANLMGIRAFRKLLPNIPHVAIFDTAFHQTMPEKAYL
                     YSLPYHYYKDYGIRKYGFHGTSHKFVSQRAAEMLDKPIEDLRIISCHIGNGASIAAID
                     GGKSIDTSMGFTPLAGVTMGTRSGNIDPALIPFIMEKTGKTAEQVLEILNKESGLLGL
                     SGTSSDLRDLSEEAESGKARSQMALDVFASKIHKYIGSYAARMHGVDVIVFTAGIGEN
                     SVEIRAKVLEGLEFMGVYWDPKKNENLLRGKEGFINYPHSPVKVVVIPTDEESMIARD
                     VMTFGGLK"
     gene            complement(3168..3668)
                     /locus_tag="NBH32_001652"
     CDS             complement(3168..3668)
                     /locus_tag="NBH32_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1515245.1"
                     /translation="MITYKNILIAVDGSHEAEWAFNRAVGVAKRNDAKLTIVNVIDSR
                     TYSSYEVYDAQFTEKSKHFAEELLNGYKEVATNAGVKDVETRLEFGSPKSIIPKKLAH
                     EINADLIMSGTSGLNAVERFIVGSVSESIVRHAPCDVLVVRTEELPADFQPQVATTQL
                     REKYQN"
     gene            3809..4927
                     /gene="ald"
                     /locus_tag="NBH32_001653"
     CDS             3809..4927
                     /gene="ald"
                     /locus_tag="NBH32_001653"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD1515246.1"
                     /translation="MKIGIPREIKNNENRVGLSPSGVHALVESGHTVLVETNAGSGSF
                     FEDVDYKEAGAEIVAEQAKVWDVDMVIKVKEPLESEYPYFKEGLVLFTYLHLANEEKL
                     TQALIDRKVISIAYETVQLPDRSLPLLSPMSEVAGRMSAQVGAEFLQKLNGGMGILLG
                     GVPGVPKGKVTIIGGGQAGTNAAKIALGLGADVTILDVNPKRLQQLDDLFGGRVHTIM
                     SNPLNIELYVKQSDLVIGAVLIPGAKAPRLVTEDMIKQMKNGSVIIDIAIDQGGIFET
                     TDKITTHDDPTYIKHGVVHYAVANMPGAVPRTSTLALNNATLPYALMLANKGYREAFK
                     SNQPLSLGLNTYKGHVTNKGVAEAFEMEYKSVEEALQL"
     gene            5051..5164
                     /locus_tag="NBH32_001654"
     CDS             5051..5164
                     /locus_tag="NBH32_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515247.1"
                     /translation="MNDIDLIHYMLLMNIPAYVFLACLLHIAFRLGQKFMS"
     gene            complement(5243..6298)
                     /locus_tag="NBH32_001655"
     CDS             complement(5243..6298)
                     /locus_tag="NBH32_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1515248.1"
                     /translation="MTKISKIIDELNNRQADAAWITTPLNVYYFTGYRSEPHERLFAL
                     LIKKDGKQVLFCPKMEVEEVKASPFTGEIVGYLDTENPFSLYPQTINKLLIESEHLTV
                     ARQKQLISGFNVNSFGDVDLTIKQLRNIKSEDEISKIRKAAELADKCIEIGVSYLKEG
                     VTEREVVNHIEQTIKQYGVNEMSFDTMVLFGDHAASPHGTPGDRRLKSNEYVLFDLGV
                     IYEHYCSDMTRTIKFGEPSKETQEIYNIVLEAETSAIQAIKPGIPLKDIDHIARNIIS
                     EKGYGEYFPHRLGHGLGLQEHEYQDVSSTNSNLLEAGMVITIEPGIYVPGVAGVRIED
                     DILVTNEGYEVLTHYEK"
     gene            6473..7162
                     /locus_tag="NBH32_001656"
     CDS             6473..7162
                     /locus_tag="NBH32_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1515249.1"
                     /translation="MKLSFHGQSTIYLEGNNKKVIVDPFISNNPKCDLNIETVQVDYI
                     VLTHGHFDHFGDVVELAKKTGATVIGSAEMADYLSSYHGVENVHGMNIGGKANFDFGS
                     VKFVQAFHSSSFTHENGIPVYLGMPMGIVFEVEGKTIYHTGDTGLFSDMSLIAKRHPV
                     DVCFVPIGDNFTMGIDDASYAINEFIKPKISVPIHYDTFPLIEQDPQQFKDAVNVGDV
                     QILKPGESVQF"
     gene            complement(7467..7880)
                     /locus_tag="NBH32_001657"
     CDS             complement(7467..7880)
                     /locus_tag="NBH32_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HCD1515250.1"
                     /translation="MYKNILLGVDTQLKNEKALKEVSKLAGEGTVVTVLNAISEQDAQ
                     ASIKAGVHLNKLTEERSKRLEKTRKALEDYGIDYDQIIVRGNAKEELLKHANSGKYEI
                     VVLSNRKAEDKKKFVLGSVSHKVAKRATIPVLIVK"
     gene            8085..9383
                     /locus_tag="NBH32_001658"
     CDS             8085..9383
                     /locus_tag="NBH32_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HCD1515251.1"
                     /translation="MTKHEQILDYIESLSIGSKISVRKIAKFLNVSEGTAYRAIKDAD
                     KMGMVATIDRVGTVRIEKRNRNEIEHLTFNEIVNIIDGQVLGGNKGITKMVSKFAIGA
                     MELKDILKYIGPKTLLIVGNREDVQIEALKRGTAILITGGFKPSNKVINFANEHDLPV
                     LSSSYDTFLVANIINKALFNQKIRKDILIVQDIMTPLDDLSVLFDTMKIADYKRMANR
                     TGHTRFPVVNESYKLVGIVTSREMINTKDDDEIDKVMTRNPIYVNAMSTVASCAHMMI
                     WEGIELIPVVSSNKKTVGVINRQDVLKSMQLLGRQPQMGETINDQIAKYITMNQDGIT
                     VEVSPLLINHYGTVSKAAFVSIIEETIQYEMRKFKKGNVMIENLNIVYIKTVPIESHI
                     TVRFGILDVGRNFAKIEVNMHSQNDKVASALVICQMFDEV"
     gene            9692..10633
                     /gene="pde2"
                     /locus_tag="NBH32_001659"
     CDS             9692..10633
                     /gene="pde2"
                     /locus_tag="NBH32_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pApA hydrolase Pde2"
                     /protein_id="HCD1515252.1"
                     /translation="MISTLNEIMKCIEDNDTIIIHRHVRPDPDAYGSQLGLKYYIQQK
                     FPQKQVFAVGEAESSLSFIGELDNIDDKTYQDALVIVCDTANAPRIDDERYSTGRKLI
                     KIDHHPAVDQYGDINLVNTNASSTSEIIYDLISHFNDEAIVNKDIASVLYLGIVGDTG
                     RFLFNNTSEHTMEIAGKLIGHDIDHNALLNKMMEKDPKMLPFQGYVLQHFELMGDGFC
                     QVKITEDVLEQFGIQPNEASQFVNTIADIKGLKIWVFAVDEGSEIRCRLRSKGQLIIN
                     DIAQDFGGGGHPNASGVSVDSWDEFEQLATALRTKLN"
     gene            10654..13851
                     /locus_tag="NBH32_001660"
     CDS             10654..13851
                     /locus_tag="NBH32_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCD1515253.1"
                     /translation="MVAYLNIHTAYDLLNSSLKIEDAVRLAVSENVDALAITDTNVLY
                     GFPKFYDTCIANNIKPIFGMTIYVTNGLNTVETVVLAKNNDGLKDLYQLSSEIKMNAL
                     EHVSFELLKRFSNNMIIIFKNVADEHRDIVQVFDSHEDTYLDHRSVLVQGIKHVWIQN
                     VCYQTRQDADTISALAAIRDNTKLDLIHDQEDFGAHFLTENEIQQLDVNQEYLTQVDV
                     IAQKCNAELKYHQSLLPQYQTPNDESAKKYLWRVLVTQLKKLELNYDVYLERLKYEYK
                     VITNMGFEDYFLIVSDLIHYAKTHDVMVGPGRGSSAGSLVSYLLGITTIDPIKFNLLF
                     ERFLNPERVTMPDIDIDFEDTRRERVIQYVQEKYGELHVSGIVTFGHLLARAVARDVG
                     RIMGFDEVTLNEISSLIPHKLGITLDEAYQIDDFKKFVHRNHRHERWFSICKKLEGLP
                     RHTSTHAAGIIINDHPLYEYAPLTKGDTGLLTQWTMTEAERIGLLKIDFLGLRNLSII
                     HQILTQVKKDLGINIDIENIPFDDQKVFELLSQGDTTGIFQLESDGVRSVLKKLKPEH
                     FEDIVAVTSLYRPGPMEEIPTYITRRHDPSKVQYLHPHLEPILKNTYGVIIYQEQIMQ
                     IASTFANFSYGEADILRRAMSKKNRAVLESERQHFIEGAKQNGYHEDISKQIFDLILK
                     FADYGFPRAHAVSYSKIAYIMSYLKVRYPNYFYANILSNVIGSEKKTAQMIEEAKKQG
                     ITILPPNINESHCFYKPSQEGIYLSIGTIKGVGYQSVKVIVDERYQNGKFKDFFDFAR
                     RIPKRVKTRKLLEALILVGAFDAFGKTRSTLLQAIDQVLDGDLNIEQDGFLFDILTPK
                     QMYEDKEELPDALISQYEKEYLGFYVSQHPVDKKFVAKQYLTIFKLSNAQNNKPILVQ
                     FDKVKQIRTKNGQNMAFVTLNDGIETLDGVIFPNQFKKYEELLSHNDLFIVSGKFDLR
                     KQQRQLIINEIQTLATFEEQKLAFAKQIIIRNKSHIDAFEEAIKATKENANDVVLSFY
                     DETIKQMTTLGYINQKDSMFNNFIQSFNPSDIRLI"
     gene            14302..15531
                     /locus_tag="NBH32_001661"
     CDS             14302..15531
                     /locus_tag="NBH32_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HCD1515254.1"
                     /translation="MSLRDEALEMHKRNQGKLEVKPNVKVTNKEELSLAYSPGVAEPC
                     KDIYEDKRKVYDYTIKGNTVAVITDGTAVLGLGNIGPEASIPVMEGKAVLFKSFAGIN
                     GMPIALNTTDTEEIIKTVKLLEPNYGGINLEDISAPRCFEIEERLKKETNIPVFHDDQ
                     HGTAIVTMAGLVNALRVVNKDIAKIKVVLNGAGAAGIAIVKLLYAYGVRNMVMCDSRG
                     AIFEGRSYGMNPTKDVVAKWTNKDKIEGSLEEVVKDADVFIGVSVANALSQDMVKSMA
                     DNPIIFAMANPNPEIIPDDAKAAGARVVGTGRSDYPNQINNVLAFPGIFRGALEVEAT
                     HINEEMKKAAVEAIADLIDSSELNEDYCIPGPFDKRVAPSVARNVAKAAMESGVARIE
                     VDPQDVYDKTMKLTDLQ"
BASE COUNT         5373 a         2245 c         2897 g         5075 t
ORIGIN      
        1 aattgtaatc aaaccataaa ttgtcgtatg atgtagttag aattttaaaa tgcaggaggt
       61 caagtatatg actgaaataa cattcaaagg tggaccaatc cacttaaaag gtcaacaaat
      121 taatgaaggt gattttgcac ctgattttac agtgttagat aatgacttaa atcaagtaac
      181 attagcagat tatgctggta aaaagaaatt aattagtgtg gtaccatcaa ttgatacagg
      241 tgtttgtgat cagcagactc gcaaattcaa ctctgaagct tctaaagaag aggggattgt
      301 gcttacaatt tcagcagact taccattcgc acaaaaaaga tggtgcgctt cagcaggttt
      361 agacaatgtc attacattaa gtgaccaccg tgacttatca tttggtgaaa actatggcgt
      421 tgttatggaa gaacttcgct tattagctcg tgcagtattt gtattagatg cagataataa
      481 agttgtttat aaagaaatcg ttagtgaagg tactgatttc ccagattttg atgctgcttt
      541 agctgcatgc aaaattattt aatcattaaa gagataaatc ttaaaatgta tacatcgtgt
      601 ccatcgttgt caacagcatt aaaatagaat tgttttctat gattgctaag acctatgggc
      661 actttttatt ggagagggac gaatatggca gaacaacaaa caattatgga acgcttgttt
      721 catacattag atgaaaaagc taaaacatta aataatgaaa atggccaaag ttttattgaa
      781 aatcttgggc tagcaatgga acaagtatat accaatgaaa gaggattgtt agaacaatcg
      841 acgttacaag atcgacgtaa agcattccaa tttgcatatt taagtttaat gcaggaagaa
      901 aagatacaag caaatcatca aattacacca gattcaattg gattgatact aggattttta
      961 gttgagcgtt ttatgaacaa ccaagaagaa ttacatattg ttgatattgc aagtggtgcc
     1021 ggtcatttaa gtgctactgt aaaagaagtg ttacctgaaa ttgcggttat gcatcattta
     1081 attgaagttg atccagtttt atcacgtgtt agtgtacatt tagcaaactt cttagaaatt
     1141 cctttcgatg tgtatcctca agatgccatc atgccactac cattagaaga agcagatatc
     1201 gttattggtg attttccagt aggctattat ccaattgatg aaagaagtaa ggagtttaag
     1261 ctaggttttg aagaaggaca tagttattca cattatttat taatagaaca agcaataaat
     1321 gcattaaaag atgctggata tgcctttcta gtggtaccaa gtaatatttt tacaggtgaa
     1381 catgtaaaac agcttgaaaa atatattgca acagagacag agatgcaagc atttttaaat
     1441 ttaccaccaa ctttatttaa aaatgaaaaa gcgcgaaaat ctatattaat tttacaaaag
     1501 aaaaaatcgg gtgaaacaaa gccagttgaa gtattattgg caaatattcc tgatttcaaa
     1561 aatccttcac aatttcaagg atttatgaca gagttaaatc agtggatgga cacaaatcgt
     1621 cctaaaaaat aatctgtaat agtatagtca taaaactgta tgaatgataa aatgagaaat
     1681 gaaatagtat aaaaagcaaa cgatggagga tcattactta tgtcaaaatt aatcttggct
     1741 atcaatgctg gtagttcatc attaaaattt caattaatta gaatgcctga agaggaatta
     1801 gtaacaaaag gtttaataga aagaatcgga ttgaaggatt caatttttac aattgaagtc
     1861 aacggggaaa aagttaaaac agtacaagat atcaaggatc acgttgaggc agttgatatc
     1921 atgttggatg cttttaaagc gcataatatt attaatgata ttaacgatat cgatggaaca
     1981 ggtcaccgtg ttgtacatgg tggtgaaaaa ttccctgaat cagtagctat tactgatgag
     2041 gttgaaaagg aaattgaaga attaagcgaa ttagcaccgc ttcacaaccc agctaacttg
     2101 atgggtattc gtgctttccg taaattatta ccaaatatcc ctcatgtggc aatttttgat
     2161 acagcattcc atcaaacaat gcctgaaaaa gcatatctat atagcttgcc atatcattat
     2221 tataaagatt atggcattcg taagtatggt ttccatggta caagccataa atttgtatca
     2281 caaagagcgg cagaaatgtt agataagcca attgaagatt tacgtattat ttcatgtcac
     2341 attggtaatg gagcatcaat tgcagctatc gatggcggta aatctattga tacatcaatg
     2401 ggcttcacac cacttgctgg tgtaacaatg ggtactcgtt ctggtaatat tgaccctgct
     2461 ttaattccat ttattatgga aaaaacaggt aaaactgctg aacaagtatt ggaaatctta
     2521 aacaaagaat caggtttatt aggattatca ggtacatcta gtgatttacg tgacttatca
     2581 gaagaagctg aatcaggtaa agcacgttca caaatggctt tagatgtatt tgcttctaaa
     2641 attcacaaat atattggatc atatgctgcg agaatgcatg gtgttgatgt tatcgtattt
     2701 acagcaggta tcggtgaaaa ctcagtagaa attcgtgcga aagttcttga aggcttagaa
     2761 tttatgggtg tttactggga tcctaagaaa aatgaaaact tactacgtgg taaagaagga
     2821 tttattaact atcctcattc accagttaaa gttgtcgtta ttccaactga tgaagaaagt
     2881 atgattgccc gtgatgttat gacattcggt ggtttaaaat aatttttaaa taaatattaa
     2941 gcgtctatct ccgctacaat tatatgtagg aggtagacgc tttttgtatt gtttgaaaat
     3001 taactagtgt aatgtctctt ttcacgaata tcttaaagaa ttaagtagtc gaagaagatt
     3061 atgaaaacat tattaagtga gttttattta taaacaataa aaaaccaagt gcagaaagtt
     3121 gcacttggtt cataatgatc aagtagttat ctaatgatca tctatattta attttgatat
     3181 ttttcacgta attgagttgt tgcaacttgt ggttggaagt ctgctggtaa ctcttcagta
     3241 cgaacaacta acacgtcaca tggcgcatga cgaacgatag attctgatac agaaccaaca
     3301 ataaatcttt ccacggcatt taagcctgat gtaccactca taatcaagtc tgcattaatt
     3361 tcatgtgcaa gctttttagg aatgatagat ttaggagagc caaactctag acgcgtttct
     3421 acatctttaa caccagcgtt agtagctact tctttataac catttaataa ttcttctgca
     3481 aaatgcttag atttttcagt aaattgagca tcataaactt cataagaaga atatgttctt
     3541 gaatcaatta cattcacaat tgttaacttc gcatcgttac gtttagcaac accaactgct
     3601 ctgttaaatg cccattccgc ttcatgtgaa ccgtcaactg cgattaaaat atttttgtaa
     3661 gtaatcatag taagtgcctc cttgttcttt cttaattata ttttaccaca attttcggaa
     3721 aagtcagtgt aataaccttt acaattttgt ggatttttga ttgcgctttc aaaatggtcg
     3781 cgttaagatg gttatggagg tggatggtat gaaaattggt ataccaaggg agattaaaaa
     3841 taatgaaaat cgtgttggtt tatcaccaag tggtgtgcac gctttagtag aaagtgggca
     3901 tactgtttta gtggaaacaa atgcgggttc aggatcgttc tttgaagatg tagattacaa
     3961 agaagcaggt gctgagattg ttgctgaaca agcaaaagtt tgggatgtgg atatggttat
     4021 taaagttaaa gaaccacttg aatctgaata tccatatttt aaagaagggc ttgtattatt
     4081 cacttatctt catttagcaa atgaagaaaa attaacacaa gctttgatag atagaaaagt
     4141 aattagcatt gcatatgaga ctgtgcagtt accagaccga tctttaccat tgttatcacc
     4201 aatgagtgag gtagcaggaa gaatgtcagc tcaagttggc gcagagttcc tacaaaaact
     4261 taatggtggt atgggaattc tactaggtgg tgtcccagga gtacctaagg gtaaagtaac
     4321 tattatcggt ggtggtcaag caggaacaaa tgcagctaaa attgcactag gactaggtgc
     4381 agatgttacg attttagatg ttaatccaaa gcgtttacaa caattagatg atttattcgg
     4441 tggacgtgta catacaatta tgtcaaatcc gttgaatatt gagttgtatg ttaaacaaag
     4501 tgatttagta ataggtgcag ttttaattcc aggtgctaaa gcgccaagac ttgtaacaga
     4561 agacatgatt aaacaaatga aaaatgggtc agttattatt gacattgcta ttgatcaagg
     4621 cggtattttt gaaacaactg ataaaattac gacacatgat gatcctacat atattaagca
     4681 tggtgtggtt cattatgcag ttgcaaatat gccaggtgca gtaccgcgta cttcgacgtt
     4741 agctttaaat aatgctacgc taccttatgc gctcatgcta gctaataaag ggtatagaga
     4801 agcatttaaa tcaaatcaac cattatcatt aggtttaaat acttacaaag gtcacgtaac
     4861 caataaaggc gttgcagagg catttgaaat ggaatataaa tctgtagaag aagcattaca
     4921 attataattt gaatttaatg aaaagggaat ggattacagc gtactaaaga atgacgttat
     4981 catctccttg gttattaaaa ttaaataatg ataaattaaa gctagaaaca atgcgaagaa
     5041 atcatcgaac atgaatgata ttgatttgat tcattacatg ttactgatga acatacctgc
     5101 atatgtgttt ctagcttgtt tgttacatat tgcatttaga cttggacaaa aatttatgtc
     5161 ctaggcgcta caatgttata ttggcagtgt ttgactgaat gagaatgcgc ttgtaacaag
     5221 cttttcattt ttgtctcact ccttattttt cgtaatgtgt taatacttca tatccttcat
     5281 tagtgacaag tatgtcatct tcaattctta cacctgcaac acctggtaca taaatacctg
     5341 gttcgattgt aatgaccatg ccagcttcta acaaattaga attagtactt gaaacatctt
     5401 gatattcatg ttcttgtaat cctagaccat gacctaagcg atgagggaaa tattcaccat
     5461 aacctttttc tgaaataata tttctagcga tatgatcgat atcttttaaa ggtattccag
     5521 gtttaattgc ttggattgca gatgtttctg cttctaatac aatattataa atttcttgtg
     5581 tttctttgct aggttcacca aatttaatgg tacgtgtcat atcgctacaa taatgctcat
     5641 aaattacacc taaatcaaat agtacatatt cattgctttt taatctgcga tctcctggtg
     5701 tgccatgagg tgatgcggca tgatctccaa ataaaaccat cgtatcaaaa ctcatttcat
     5761 tgacgccgta ttgtttgatt gtttgctcaa tatggttgac tacttcacgt tcagtcacac
     5821 cttcttttaa ataagaaaca cctatttcga tacacttatc tgctaactca gcagctttac
     5881 gtattttact aatttcatct tcggatttaa tatttctcaa ttgtttgatt gttaaatcaa
     5941 catctccgaa tgaattgaca ttgaaaccag agattaattg tttttggcgt gctactgtta
     6001 agtgctcgct ttcaattagt aatttattga ttgtttgagg ataaagtgaa aaaggatttt
     6061 cagtgtctaa atatccaacg atttcacctg tgaaaggtga tgctttgact tcttcgactt
     6121 ccatttttgg acaaaatagt acttgtttcc catctttctt aatcaataat gcaaataatc
     6181 tttcatgggg ttcgctacgg tatccagtaa agtaatatac attcaacggt gttgtaatcc
     6241 atgctgcatc agcttgtcga ttgttcagtt cgtctattat ttttgatatt tttgtcattt
     6301 tataaacact cctcaacata atattacttc cattttatgc caattattat tagatttcaa
     6361 aatcaagcta attcattaac tagattttca atttttaaaa tttgagaatt tgttataatt
     6421 aagaggcaaa agggtactaa atttatatag caattgatag gaggatgctc aaatgaagtt
     6481 atcatttcat ggtcaatcaa cgatttattt agaagggaac aacaaaaaag ttatcgtaga
     6541 tccgtttatt tcaaataacc ctaagtgtga tttgaatatt gaaacagtgc aggtagacta
     6601 tatcgtttta acgcacggtc attttgatca ctttggtgat gttgtggaac tagcaaagaa
     6661 aacaggagca acagtaattg gaagtgcaga aatggctgac tatctttctt catatcacgg
     6721 tgttgaaaat gttcatggta tgaatattgg tggtaaagca aactttgatt tcggaagcgt
     6781 taaatttgtt caagcatttc atagttcaag ttttacacat gaaaatggta ttccagttta
     6841 tttaggtatg ccaatgggta tcgtgtttga agttgaaggg aaaacaattt atcatactgg
     6901 tgatacaggt ctgtttagtg acatgtcact tattgcgaaa cgtcatccag ttgatgtatg
     6961 ttttgtacca attggagata attttacgat gggtattgat gatgcaagtt atgctattaa
     7021 tgaatttatt aaaccaaaaa tttctgtacc gattcattat gatacattcc cattaatcga
     7081 acaagatcca caacaattta aagatgctgt taatgtaggg gacgtgcaaa ttttaaaacc
     7141 aggtgaatct gttcagtttt aaatagaata tagattttac actaaaaaac acctcgctaa
     7201 tctatgtgga agttatcgtc tgcatatttt tagtgggtgt ttttatattc tactaattca
     7261 gttacgacgt ggacggggga catcagtggt tctttatcat ttctgaccta ctctcgtaat
     7321 tttagtttta tacaattaga cattgattat aaatttacta cgatataaaa aatccagaac
     7381 cacagtcata atcatttctt gtgtttactt agtcgtttga gactgcatca tgattgaaag
     7441 attatttgtg attctggata aaaattttat ttaacgatta atacagggat agtcgcacgt
     7501 tttgctactt tgtggctgac acttccaagt acaaatttct ttttgtcttc tgctttacgg
     7561 ttacttaaaa caacaatttc gtatttaccg ctattagcat gttttaatag ttcttctttt
     7621 gcattaccac gaacaattat ttggtcataa tcaataccat aatcttctaa agctttgcgt
     7681 gttttttcca atcgcttgct tcgttcttca gtaagtttgt ttaaatgaac acctgcttta
     7741 attgatgctt gagcatcttg ttcgctgatt gcgtttaaaa ctgttacgac tgtaccttcg
     7801 ccagctaatt tagacacttc ttttagtgct ttttcatttt ttaactgagt gtctacacca
     7861 agtaatatat ttttatacat tagtacatcc ctcctcactt acattctaat aaataataat
     7921 ggatttttcc atttaaaatt aaagattttg tgatctttgt gtttgttgta tattttcaaa
     7981 aaataaaata ggtgaaacga tcattatttc atttcacttc aatgcttttt atccaaaata
     8041 aagttataat gtagttaata ctgttcgaaa agaagaggta aagaatgaca aaacatgaac
     8101 aaattttaga ttatattgaa tcgttatcta taggctctaa aatttcggtg cgtaaaatcg
     8161 ctaaattttt aaacgtatct gaagggacag catatcgtgc aattaaagat gctgataaaa
     8221 tgggcatggt cgcaacgata gatcgtgttg gtacggttcg aatcgaaaaa cgtaatcgta
     8281 atgaaattga acatttaact tttaatgaaa ttgtcaatat tattgatggc caagtgttag
     8341 gtggcaataa aggtatcact aagatggtat ctaaatttgc aattggagca atggaactca
     8401 aagatatttt aaaatatatt ggtccaaaga cattattgat tgtcggaaat cgcgaagatg
     8461 tgcagattga agctttaaaa agaggtacgg ctatattaat tactggtgga tttaagccat
     8521 ctaataaagt tattaatttt gcgaatgaac atgatttacc tgttctatca tcaagttatg
     8581 atacattcct agttgcaaat attatcaaca aagcactttt taatcagaaa atacgaaaag
     8641 atatcttaat agttcaagat ataatgacgc cattagatga tttatctgtg ctgtttgata
     8701 cgatgaaaat agcagattat aagcgtatgg cgaatcgaac tggccataca agatttccgg
     8761 tagtcaatga gtcttataaa ttagtaggta ttgtaacaag tcgagagatg attaatacaa
     8821 aggacgacga cgaaatagat aaagtgatga cgcgaaatcc tatttatgta aatgctatga
     8881 gtacagtagc aagttgtgcg catatgatga tttgggaagg tattgaattg attcctgtcg
     8941 tttcgagtaa taaaaagaca gtgggtgtga ttaatagaca agatgtttta aagtctatgc
     9001 aacttttagg aagacagcct cagatgggtg aaacaattaa cgatcaaatc gccaaatata
     9061 taacaatgaa tcaagatgga attacagtag aagtttcacc acttttaatc aatcattacg
     9121 gaacagtaag taaagcggca tttgtaagta ttatagaaga aacaatacaa tatgaaatgc
     9181 gtaaatttaa aaaaggtaat gtcatgatag agaacttaaa tatcgtgtat attaaaacag
     9241 tacctattga atcacatata acagtgcgct ttggcatact agacgtcgga agaaacttcg
     9301 ccaaaataga agtgaacatg catagtcaaa acgacaaagt agcgagcgct ctcgtgatat
     9361 gccaaatgtt tgacgaagta taggaactta gcagagaaaa taggcataag atagatttcg
     9421 cacaaacgtt ggagcccaac ttgcacatta tagtaagctg acattacgtc agcttctgtg
     9481 ttgtggccca tcccaacttg cattgtttgt agaatttctt ttcgaaattc tctatgttgg
     9541 ggcccctgaa ctaaatagta tgaaaatata tatctaaata attaaaacgg gcaaatagtt
     9601 taatatggta gtaacaattt ttataattta gatattttga tatagactaa tgcttaaatt
     9661 aagcaacttt tctaaaaaag ggagtatgaa aatgattagt actttgaatg aaattatgaa
     9721 atgtatcgaa gacaacgata caattattat acacagacat gttagaccag atcctgatgc
     9781 atatggttca caacttggtt taaaatacta tattcagcag aaatttccgc aaaagcaagt
     9841 atttgcagtg ggtgaagcgg aatcatcatt aagttttatt ggagagttag ataacattga
     9901 tgataaaaca tatcaagatg cgcttgtaat tgtatgtgat actgccaatg ctccacgaat
     9961 tgacgatgaa cgttatagta caggtcgtaa acttattaaa atagatcatc atcctgcagt
    10021 tgatcagtat ggtgatatta atttagttaa tacgaacgcg tcatctacaa gtgaaatcat
    10081 ttacgattta atctcacatt ttaatgatga agcaattgtt aataaagata tcgcgagcgt
    10141 tttatatctt ggtatcgtcg gtgatactgg gcgattcctt tttaacaata cctcagaaca
    10201 tactatggaa attgctggaa agttaattgg gcatgatatt gatcataatg cgcttttaaa
    10261 taaaatgatg gagaaggatc caaaaatgtt gccgtttcaa ggttatgttt tacaacattt
    10321 cgaacttatg ggtgatggat tctgccaagt taaaataact gaagatgtat tggagcaatt
    10381 cggtattcag ccaaatgaag catctcagtt tgttaataca attgctgaca tcaaaggctt
    10441 gaaaatatgg gtgtttgcag ttgatgaggg tagtgaaata agatgtcgct tacgctctaa
    10501 agggcaattg attattaatg atattgcgca agattttggt ggcggtggtc atccgaatgc
    10561 gtcaggagtt tcagtggaca gctgggatga atttgagcaa cttgctacag ctttacgcac
    10621 aaaacttaac taatagaaag gagccattca atcatggtgg catatttaaa tattcatacg
    10681 gcttatgatt tgttaaattc aagcttaaaa atagaagatg ccgtaagact tgctgtgtct
    10741 gaaaatgttg atgcacttgc cataactgac accaatgtat tgtatggctt tcctaaattt
    10801 tatgatacat gtattgccaa taatataaag ccgatttttg gtatgacaat atacgtgaca
    10861 aatggattaa atacagtcga aacagttgtt ctagctaaaa ataatgatgg gttaaaagat
    10921 ttgtatcaac tatcatcgga aataaaaatg aatgcattag aacatgtgtc gtttgaatta
    10981 ttaaaacgat tttctaacaa tatgattatc atttttaaaa atgttgcaga cgaacatcgt
    11041 gacattgttc aagtttttga ttcgcatgaa gatacgtatt tagatcatcg aagtgtttta
    11101 gttcagggta taaagcacgt atggattcaa aatgtttgtt accaaacacg tcaagatgca
    11161 gatacaattt ctgcactcgc agcgataaga gacaatacaa aattagactt aattcatgat
    11221 caagaagatt ttggtgcgca ttttttaact gaaaatgaga ttcagcaact tgatgtgaat
    11281 caagaatatt taacgcaggt tgatgttata gctcaaaagt gtaatgcaga attaaaatat
    11341 catcaatctc tacttcctca atatcagaca cctaatgatg aatcagctaa aaaatatttg
    11401 tggcgtgtct tagttacaca attaaaaaaa ttagaactta attatgacgt ctatttagag
    11461 cgattgaaat atgagtataa agttattact aatatgggtt ttgaagatta tttcttaata
    11521 gtaagtgact taatccatta tgcgaagaca catgatgtga tggtaggtcc aggtcgtggt
    11581 tcttcagctg gctcactggt cagttattta ttgggaatta caacgattga tcctattaaa
    11641 ttcaatctat tatttgaacg ctttttaaac ccagaacgtg taacaatgcc tgatattgat
    11701 attgactttg aagatacacg ccgagaaagg gtcattcagt acgtccaaga aaaatatggc
    11761 gagctacatg tatctggaat tgtgactttc ggtcatctgc ttgcaagagc ggttgctaga
    11821 gatgttggac gaattatggg gtttgatgaa gttacattaa atgaaatttc aagtttaatc
    11881 ccacataaat taggaattac acttgatgaa gcatatcaaa ttgacgattt taaaaagttt
    11941 gtacatcgaa accatcgaca tgaacgctgg ttcagtattt gtaaaaagtt agaaggttta
    12001 ccaagacata catctactca tgcggcagga attattatta atgaccaccc attatatgaa
    12061 tatgcccctt taacgaaagg ggatacagga ttattaacgc aatggacaat gactgaagcc
    12121 gaacgaattg ggttattaaa aatagatttt ctagggttga gaaacttatc gattattcat
    12181 caaatcttaa cacaagtcaa aaaagattta ggtattaata ttgatatcga aaatattccg
    12241 ttcgatgatc aaaaagtgtt tgaattgttg tcgcaaggag atacaactgg catattccaa
    12301 ttagagtctg acggtgtaag aagtgtatta aaaaaattaa agccggaaca ctttgaagat
    12361 attgtggctg taacttcttt gtatagacca ggtccaatgg aagaaattcc aacttacatt
    12421 acaagaagac atgatccaag caaagttcaa tatttacatc cacatttaga acctatatta
    12481 aaaaatactt acggtgttat tatttatcaa gagcaaatta tgcaaatagc gagcacattt
    12541 gcaaacttca gttatggtga agcggatatt ttaagaagag caatgagtaa aaaaaataga
    12601 gctgttcttg aaagtgagcg tcaacatttt atagaaggtg caaagcaaaa tggttatcac
    12661 gaagacatta gtaagcaaat atttgatttg attctgaaat ttgctgatta tggttttcct
    12721 agagcacatg ctgtcagcta ttctaaaatt gcatacatta tgagctattt aaaagtccgt
    12781 tatccaaatt atttttacgc aaacatttta agtaatgtta ttggaagtga gaagaaaact
    12841 gctcaaatga tagaagaagc aaaaaaacaa ggtatcacta tattgccacc gaacattaac
    12901 gaaagtcatt gtttttataa accttcccaa gaaggcattt atttatcaat tggtacaatt
    12961 aaaggtgttg gttatcaaag tgtgaaagtg attgttgatg aacgttatca gaacggcaaa
    13021 tttaaagatt tctttgattt tgctagacgt ataccaaagc gagtcaaaac gagaaagtta
    13081 cttgaagcac tgattttagt gggagcgttt gatgcttttg gtaaaacacg ttcaacgttg
    13141 ttgcaagcta ttgatcaagt gttggatggc gatttaaaca ttgaacaaga tggtttttta
    13201 tttgatattt taacgccaaa acagatgtat gaagataaag aagaattgcc tgatgcactt
    13261 attagtcagt acgaaaaaga atatttagga ttttatgttt cgcaacaccc agtagataaa
    13321 aagtttgttg ccaaacaata tttaacgata tttaaattga gtaacgcaca gaataataag
    13381 cctatattag ttcagtttga taaagttaaa caaatccgaa ctaaaaatgg ccaaaatatg
    13441 gcattcgtca cattaaatga tggcatcgaa actttagatg gtgtgatttt ccctaatcag
    13501 tttaaaaagt acgaagagtt gttatcacat aatgacttgt ttatagttag cgggaaattt
    13561 gaccttagaa agcaacaacg tcaattaatt ataaatgaga ttcagacatt agccactttt
    13621 gaagaacaaa aattagcatt tgccaaacaa attataatta gaaataaatc acatatagat
    13681 gcgtttgaag aggcgattaa agctacgaaa gagaatgcta atgatgttgt gttatccttt
    13741 tatgatgaaa cgattaaaca aatgactact ttaggctata ttaatcaaaa agatagtatg
    13801 tttaataatt ttatacaatc ctttaaccct agtgatatta ggcttatata acttttatca
    13861 actaataaat tatgatatag taaactgatg gttagatatt ttttaaccat cagtttgcgt
    13921 ttatattagt tttttatgct tattattttt atgagtttca ctttacatta ttgattaatc
    13981 cataagaata attagcacaa aaagcagtat acataaattg agtaaagaat tttgtcgata
    14041 tatcgtgcga aaaaataata actttttgat atattggttt ttgctatcca ttataataat
    14101 taaaggtata tctttagata gtatggttta atgtatcttt atatatatta ttttattatc
    14161 gtttatcgct attggtgtta cataattaaa accataaatt cttactcatt cagatttacc
    14221 caatattttt acttttataa tgtaatgcgt tttatccaag ttattttttt aaaaataaat
    14281 attgaattag gggctgattt catgtcatta agagatgaag cattggaaat gcacaaacgt
    14341 aatcaaggta aattagaagt taaaccaaat gtaaaagtta ctaataaaga ggaattaagt
    14401 ttagcatatt cacctggcgt tgctgaaccg tgtaaagata tatatgaaga taaaagaaaa
    14461 gtgtatgatt acacaatcaa agggaataca gttgcagtta ttactgatgg aacagcggta
    14521 ttaggtttag gtaacattgg acctgaagca agtattcctg taatggaagg taaagcagta
    14581 ttattcaaaa gcttcgctgg tatcaatggg atgcctattg cgttaaatac aactgatacc
    14641 gaagaaatca ttaaaacagt taagttgtta gaacctaatt atggtggtat taatttagag
    14701 gatatttcgg caccacgttg ttttgaaatt gaagaacgat tgaaaaaaga aactaatatt
    14761 ccggtattcc atgacgatca acatggtaca gcaattgtaa caatggcagg tttggtaaat
    14821 gcattgagag ttgttaacaa agatattgct aaaataaaag ttgtactaaa tggtgctggt
    14881 gcagcaggaa tagccattgt taaattacta tacgcgtatg gtgtaagaaa tatggttatg
    14941 tgtgactcaa gaggcgcaat ttttgaagga cgttcatatg gtatgaatcc tacgaaagat
    15001 gttgtagcta aatggacaaa taaagataag attgaagggt ctttagaaga agtcgtaaaa
    15061 gacgcagatg tatttatcgg agtttctgta gctaatgcgc tgtcacaaga tatggttaag
    15121 agtatggcag ataatccaat tatatttgca atggctaatc caaatcctga aataatacct
    15181 gatgatgcca aagcggcagg tgcacgcgtt gttggtactg gacgttcaga ttatcctaac
    15241 caaattaata atgtattagc tttccctggt atttttagag gtgcattaga ggttgaagct
    15301 acacatataa atgaagaaat gaaaaaagca gctgtagaag cgattgctga tttaatcgat
    15361 agttctgaat taaatgaaga ctactgtatc ccaggaccgt ttgataaacg tgtagcgcca
    15421 tcagttgctc gtaatgttgc taaagcggca atggaatctg gagtagctag gattgaagtt
    15481 gatccgcaag atgtgtatga taaaacaatg aaacttacag atttacaata aaaataatta
    15541 acgtttaatt aaattgctta aaagttagca ataaagattc attaaaatga
//
LOCUS       DAHHOJ010000063        15577 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.063, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000063 DAHHOJ010000000
VERSION     DAHHOJ010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15577)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15577)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15577
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.063"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(113..346)
                     /gene="nrdH"
                     /locus_tag="NBH32_001662"
     CDS             complement(113..346)
                     /gene="nrdH"
                     /locus_tag="NBH32_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCD1515255.1"
                     /translation="MSEIIVYTQNDCPPCTFVKNYLNEHQIDFEERNINNQQYRNEMI
                     DFDAFSTPFILLNGNPMYHVDLDEINKVLNIQD"
     gene            544..1905
                     /locus_tag="NBH32_001663"
     CDS             544..1905
                     /locus_tag="NBH32_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCD1515256.1"
                     /translation="MDTVEISRFLTAMTLAVHIIFATIGVGMPLMFAIAEFLGIRKND
                     LQYIAMAKRWAKAYTITVAVGVVTGTIIGLQLSLIWPTFMEMGGHVIALPLFMETFAF
                     FFEAIFLSIYLYTWDRFKNKWTHFLISIPVIIGGSFSAFFITSVNSFMNTPAGFELKN
                     GKMVNVQPIEAMFNPSFIVRSFHVITTAGMTMAFVIASIAAFKLLRNRQPKDTVYHKK
                     ALKISMIVGFFSTLLSMLAGDLSAKFLHKFQPEKLAAYEWHFDTSSHAKLLLFGVLDE
                     KTQQVKGAIELPGLLSFLADNSVKTKVQGLNDFPKSLHPPMIVHYFFDLMVTMGILCF
                     VISGVYVLTLMFKKLRKFSTHKWMLYGILLTGPASMLAIEFGWFLTEMGRQPWIVRGY
                     MRVAEAATQAGGITFVTILFGILYIILMYTCAYVLIRMFKNKPAYEDVNRLAKKQGGE
                     IEK"
     gene            1902..2921
                     /locus_tag="NBH32_001664"
     CDS             1902..2921
                     /locus_tag="NBH32_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCD1515257.1"
                     /translation="MIYAFIGITVLWLFLFCYIIIASIDFGAGFFALHSKLTGDEKKI
                     NHLISRYLNPVWEVTNVFFVFFFVGFVGFFPESIKYLGTVLLIPGSIALIMISLRNSF
                     YAFENYGQDTKLAWMIMYGVSGLLIPASLSTALTITEGGYINVRNNVIDLDWVQLLLS
                     PFAWSVVFLAIISVLYISSGFLTYYAKKANDEPAYNLTRQWHIFLGPPMIIICLFVFL
                     SLRIQNSEHFYSAVFDYWWMFAISFLFFALASLLTFFKKKHGLAFVFVILQMMFAFFG
                     YGISKLPYLLYPFVKITDAYVNPEMGWTLVIVFILGLLLLLPSLILLLRLFVFDKEYV
                     EGKKS"
     gene            3054..3716
                     /locus_tag="NBH32_001665"
     CDS             3054..3716
                     /locus_tag="NBH32_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HCD1515258.1"
                     /translation="MGKEYVVIGLGRFGGSIVRELNALDMDVMAIDHDENRVNEYSDI
                     ATHAVVADTTDEAVMKSLGIRNFDHVIVAIGENIQSSTLTTLILKELGVKKVTAKAQN
                     DYHAKILNKIGADTVVHPERDMGRRIAHNVASASVLDYLELADEHSIVELKATEKMAG
                     KSIIDLDIRAQYGINIIAIKRGKEFIISPNPNINLEIGDILIMIGHDNDLNRFEKNIA
                     TR"
     gene            complement(4124..5821)
                     /locus_tag="NBH32_001666"
     CDS             complement(4124..5821)
                     /locus_tag="NBH32_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1515259.1"
                     /translation="MKQLHPNEVGVYALGGLGEIGKNTYAVEYKDEIVIIDAGIKFPD
                     DNLLGIDYVIPDYTYLVQNQDKIVGLFITHGHEDHIGGVPFLLKQLNIPIYGGPLALG
                     LIRNKLEEHHLLRTAKLNEINEDSVIKSKHFTISFYLTTHSIPETYGVIVDTPEGKVV
                     HTGDFKFDFTPVGKPANIAKMAQLGEEGVLCLLSDSTNSLVPDFTLSEREVGQNVDKI
                     FRNCKGRIIFATFASNIYRVQQAVEAAIKNNRKIVTFGRSMENNIKIGMELGYIKAPP
                     ETFIEPNKINTVPKHELLILCTGSQGEPMAALSRIANGTHKQIKIIPEDTVVFSSSPI
                     PGNTKSINRTINSLYKAGADVIHSKISNIHTSGHGSQGDQQLMLRLIKPKYFLPIHGE
                     YRMLKAHGETGVECGVEEDNVFIFDIGDVLALTHDSARKAGRIPSGNVLVDGSGIGDI
                     GNVVIRDRKLLSEEGLVIVVVSIDFNTNKLLSGPDIISRGFVYMRESGQLIYDAQRKI
                     KTDVISKLNQNKDIQWHQIKSSIIETLQPYLFEKTARKPMILPVIMKVNEQKESNNK"
     gene            complement(5821..6039)
                     /locus_tag="NBH32_001667"
     CDS             complement(5821..6039)
                     /locus_tag="NBH32_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HCD1515260.1"
                     /translation="MAVFKVFYQHNRDEVIVRENTQSLYVEAQTEEQVRRYLKDRNFN
                     IEFITKLEGAHLDYEKENSEHFNVEIAK"
     gene            complement(6068..6190)
                     /locus_tag="NBH32_001668"
     CDS             complement(6068..6190)
                     /locus_tag="NBH32_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515261.1"
                     /translation="MNDMMNLVINQIVIKLNYRDDWNEFIDIYSNTILLGLTIN"
     gene            complement(6521..7072)
                     /locus_tag="NBH32_001669"
     CDS             complement(6521..7072)
                     /locus_tag="NBH32_001669"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCD1515262.1"
                     /translation="MLTMKDIIRDGHPTLRQKAAELELPLTKEEKETLIAMREFLVNS
                     QDEEIAKRYGLRSGVGLAAPQINISKRMIAVLIPDDGSGKSYDYMLVNPKIVSHSVQE
                     AYLPTGEGCLSVDDNVAGLVHRHNRITIKAKDIEGNDIQLRLKGYPAIVFQHEIDHLN
                     GVMFYDHIDKDHPLQPHTDAVEV"
     gene            7437..8063
                     /locus_tag="NBH32_001670"
     CDS             7437..8063
                     /locus_tag="NBH32_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkyA family protein"
                     /protein_id="HCD1515263.1"
                     /translation="MKFGKTIAVVLASSVLLAGCTTDKKEIKAYLKQVDKIKDDEEPI
                     KTVGKKIAELDEKKKKLTEDVNSKDTTVRGKAVKDLIKNADDRLKEFEKEEDAIKKSE
                     QDFKKAKSHVDNIDNDVKRKEVKQLDDVLKEKYKLHSDYAKAYKKAVNSEKTLFKYLN
                     QNDATQQGVNEKSKAIEENYKKLKEVSDKYTKVLNKVGKEKQDVDQFK"
     gene            8234..9346
                     /gene="pdhA"
                     /locus_tag="NBH32_001671"
     CDS             8234..9346
                     /gene="pdhA"
                     /locus_tag="NBH32_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002467471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HCD1515264.1"
                     /translation="MAPKLQAQFDAVKVLNDTQSKFEMVQILDENGNVVNEDLVPDLT
                     DEQLVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILP
                     GYRDVPQIIWHGLPLTEAFLFSRGHFKGNQFPEGVNALSPQIIIGAQYIQAAGVAFAL
                     KKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA
                     ETLAQKAIAVGIPGIQVDGMDALAVYQATKEARDRAVAGEGPTLIETMTYRYGPHTMA
                     GDDPTRYRTSDEDAEWEKKDPLVRFRKFLENKGLWNEDKENEVIERAKADIKAAIKEA
                     DNTEKQTVTSLMEIMYEDMPQNLAEQYEIYKEKESK"
     gene            9350..10327
                     /locus_tag="NBH32_001672"
     CDS             9350..10327
                     /locus_tag="NBH32_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017637695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HCD1515265.1"
                     /translation="MAQMTMVQAINDALKTELKNDQDVLIFGEDVGVNGGVFRVTEGL
                     QKEFGEDRVFDTPLAESGIGGLAMGLAVEGFRPVMEVQFLGFVFEVFDAIAGQIARTR
                     FRSGGTKTAPVTIRSPFGGGVHTPELHADNLEGILAQSPGLKVVIPSGPYDAKGLLIS
                     SIRSNDPVVYLEHMKLYRSFREEVPEEEYTIDIGKANVKKEGNDISIITYGAMVQESM
                     KAAEELEKDGYSVEVIDLRTVQPIDVDTIVASVEKTGRAVVVQEAQRQAGVGAAVVAE
                     LSERAILSLEAPIGRVAAADTIYPFTQAENVWLPNKNDIIEKAKETLEF"
     gene            10418..11710
                     /locus_tag="NBH32_001673"
     CDS             10418..11710
                     /locus_tag="NBH32_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HCD1515266.1"
                     /translation="MAFEFRLPDIGEGIHEGEIVKWFVKAGDTIEEDDVLAEVQNDKS
                     VVEIPSPVSGTVEEVMVEEGTVAVVGDVIVKIDAPDAEDMQFKGHDDDSSSKEEPAKE
                     EAPAEQAPVATQTEEVDENRTVKAMPSVRKYAREKGVNIKAVSGSGKNGRITKEDVDA
                     YLNGGAPTASNESAASTTSEEVAETPAAPAAVSLEGDFPETTEKIPAMRRAIAKAMVN
                     SKHTAPHVTLMDEIDVQALWDHRKKFKEIAAEQGTKLTFLPYVVKALVSALKKYPALN
                     TSFNEEAGEIVHKHYWNIGIAADTDRGLLVPVVKHADRKSIFQISDEINELAVKARDG
                     KLTADEMKGATCTISNIGSAGGQWFTPVINHPEVAILGIGRIAQKPIVKDGEIVAAPV
                     LALSLSFDHRQIDGATGQNAMNHIKRLLNNPELLLMEG"
     gene            11714..13120
                     /gene="lpdA"
                     /locus_tag="NBH32_001674"
     CDS             11714..13120
                     /gene="lpdA"
                     /locus_tag="NBH32_001674"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009383646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCD1515267.1"
                     /translation="MVVGDFPIETDTIVIGAGPGGYVAAIRAAQLGQKVTIVEKGNLG
                     GVCLNVGCIPSKALLHASHRFVEAQHSENLGVIAESVSLNFQKVQEFKSSVVNKLTGG
                     VEGLLKGNKVNIVKGEAYFVDNNSLRVMDEKSAQTYNFKNAIIATGSRPIEIPNFKFG
                     KRVIDSTGALNLQEVPGKLVVVGGGYIGSELGTAFANFGSEVTILEGAKDILGGFEKQ
                     MTQPVKKGMKEKGVEIVTEAMAKSAEETDNGVKVTYEAKGEEKTIEADYVLVTVGRRP
                     NTDELGLEELGVKFADRGLLEVDKQSRTSISNIYAIGDIVPGLPLAHKASYEAKVAAE
                     AIDGQAAEVDYIGMPAVCFTEPELATVGYSEAQAKEEGLAIKASKFPYAANGRALSLD
                     DTNGFVKLITLKEDDTLIGAQVVGTGASDIISELGLAIEAGMNAEDIALTIHAHPTLG
                     EMTMEAAEKAIGYPIHTM"
     gene            13288..13563
                     /locus_tag="NBH32_001675"
     CDS             13288..13563
                     /locus_tag="NBH32_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HCD1515268.1"
                     /translation="MEYEYPIDLDWSNEEMISVINFFNHVEKYYESGVTAGDFMGAYK
                     RFKEIVPAKAEEKQIFNTFEKSSGYNSYKAVQDVKTHSEEQRVTAKK"
     gene            13707..14246
                     /locus_tag="NBH32_001676"
     CDS             13707..14246
                     /locus_tag="NBH32_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1515269.1"
                     /translation="MNIGNKIKNLRRIKNLTQEELAERTDLSKGYISQIESEHASPSM
                     ETFLNIIEVLGTTPSEFFKDSENEKVLYKKEEQVIYDEYDEGYILNWLVSKSNEYDME
                     PLILTLKPGASYKNFNPSESDTFIYCMSGQITLNLGKEVYQAQEEDVLYFKARDNHRL
                     SNESNNETRILIVATASYL"
     gene            14259..15353
                     /locus_tag="NBH32_001677"
     CDS             14259..15353
                     /locus_tag="NBH32_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515270.1"
                     /translation="MEPLLSLKSVSKSYDDLNILDDIDIDIESGYFYTLLGPSGCGKT
                     TILKLIAGFEYPDSGEVIYQNKPIGNLPPNKRKVNTVFQDYALFPHLNVYDNIAFGLK
                     LKKLSKTEIDQKVTEALKLVKLSGYEKRNINEMSGGQKQRVAIARAIVNEPEILLLDE
                     SLSALDLKLRTEMQYELRELQSRLGITFIFVTHDQEEALALSDFLFVLKDGKIQQFGT
                     PTDIYDEPVNRFVADFIGESNIVEGRMVRDYVVNIYGQDFECVDMGIPENKKVEVVIR
                     PEDISLIKAEEGLFKATVDSMLFRGVHYEICCIDNKGYEWVIQTTKKAEVGSEVGLYF
                     DPEAIHIMVPGETEEEFDKRIESYEEVDNA"
     gene            15346..>15577
                     /locus_tag="NBH32_001678"
     CDS             15346..>15577
                     /locus_tag="NBH32_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515271.1"
                     /translation="MRNTNKFLLIPYLLWMVIFIIVPVVLLIYFSFLDINGHFSFTNY
                     QQIFTTKYLKMFAYSILYVALITIITLAISYPA"
BASE COUNT         5358 a         2296 c         2883 g         5040 t
ORIGIN      
        1 aaacaactat gtgtcattaa caaaatgttg ttctttaatt aatgttagtt aatttaaaaa
       61 tcgatattta atttatgata gctataaatg atgaagttga tttagatgaa aattaatctt
      121 ggatatttaa tactttgttg atttcatcaa gatcaacatg gtacattgga ttgccattca
      181 acaaaataaa cggagttgaa aaagcatcaa aatctatcat ttcgtttcga tattgttgat
      241 tgttgatatt tctctcttca aaatcaattt gatgctcatt tagataattt tttacaaatg
      301 tacaaggtgg acaatcattc tgcgtataaa cgattatttc tgacatgtat catgctcctt
      361 cgtgctttat tctaatgtaa tatacacaat tatacattga aattcaaatt tgtgaacaca
      421 ttgtggactg acataaatta tacacaattt catagcgcat aatgttttaa aaatacacat
      481 tatagcaatt ataattaagt tcattgtgtt gtcatcaaaa aaagaaaaag gtgatgtttt
      541 taaatggata cagttgaaat cagtcggttt ttgacagcta tgactttagc agttcatatc
      601 atttttgcaa cgattggtgt tggtatgcct ttaatgttcg caattgcaga atttttaggt
      661 attcgcaaaa atgatcttca atatatagct atggccaaaa gatgggctaa agcttataca
      721 attactgtag cagtgggagt tgttacaggt acaattatag gacttcaatt atcattgatt
      781 tggcctacat ttatggaaat gggtggacac gttattgcac ttccattatt tatggaaaca
      841 tttgcgttct tctttgaagc tattttctta agtatatatt tatatacttg ggatcgtttt
      901 aaaaataaat ggacacattt cttaattagt ataccagtaa ttattggtgg ctctttctca
      961 gcattcttca ttacttcagt gaattcattt atgaatacgc ctgcaggttt tgagttgaag
     1021 aatggaaaga tggtcaatgt tcaacctata gaagcgatgt ttaacccatc gtttatagtt
     1081 cgatcatttc acgtaattac tacagctggt atgacgatgg catttgttat tgcatcaata
     1141 gcagctttta aattattgcg taatcgtcaa cctaaagata ctgtctacca taagaaagct
     1201 ttgaaaatat ctatgatagt tggattcttt tcaacattac tttctatgtt ggcaggggat
     1261 ttatctgcaa aatttttgca taaattccaa cctgaaaaat tagcagctta tgaatggcat
     1321 ttcgatacat cttcccatgc caaattatta ttatttggtg tgttagatga aaagactcag
     1381 caagttaaag gtgcgattga attacctgga ctattgagtt tcttagcaga taatagtgtc
     1441 aaaactaaag tgcaagggtt aaatgatttt ccaaaaagtt tacatccgcc tatgattgtc
     1501 cattatttct ttgatttaat ggtaacgatg ggaattttat gttttgtcat ttcaggtgtt
     1561 tatgtcttaa ctttaatgtt taaaaagctt agaaagtttt ctactcataa atggatgctt
     1621 tacggaatat tattaaccgg accagcttca atgctagcta tagaatttgg atggttctta
     1681 acagagatgg gtagacagcc ttggattgtt cgtggttata tgcgcgtggc agaagcagca
     1741 acacaagcag gcggaataac cttcgttaca attttatttg gcatattgta catcatttta
     1801 atgtatacat gtgcatacgt attaattcgt atgtttaaaa ataaaccggc gtatgaagat
     1861 gtaaatcgct tagccaagaa gcaaggagga gaaatagaaa aatgatttat gcatttatag
     1921 gtataacagt gttatggttg tttttatttt gctatatcat tattgcttct atagattttg
     1981 gggcaggttt cttcgcattg cattcaaagt taactggtga tgaaaagaaa attaatcact
     2041 taatttcacg ttatttgaac ccagtttggg aagttacgaa tgtattcttt gttttcttct
     2101 tcgtaggatt cgtaggtttc tttccagagt caatcaaata tttaggcacg gtattgttaa
     2161 taccaggttc gatagcactg attatgatat cgttgagaaa cagtttttat gcatttgaaa
     2221 attatggtca agatacaaaa ttagcatgga tgatcatgta tggggtaagt ggattattaa
     2281 ttccagcttc attatctact gctttaacta ttacagaagg tggctatatt aatgttcgaa
     2341 acaatgttat cgatctagat tgggtgcagt tactattaag cccatttgct tggtctgtag
     2401 tattcttggc aattatttca gttttatata tttcatcagg atttttgaca tattatgcta
     2461 aaaaagcaaa tgacgaacca gcatataatt taacaagaca atggcacata tttttagggc
     2521 cgccgatgat tatcatttgt ttattcgtat ttctatcatt acgcattcaa aattctgaac
     2581 atttttattc agctgttttt gactactggt ggatgtttgc tataagtttc ttattctttg
     2641 cattagcttc attgttaaca ttctttaaga aaaaacatgg tttggctttt gtatttgtta
     2701 ttttacaaat gatgttcgcg ttctttggct atggaattag taaattgcct taccttttat
     2761 atccgtttgt aaaaattaca gatgcatatg ttaatccaga aatgggctgg acattagtga
     2821 ttgtctttat tttaggttta cttttattac ttccatcgtt aatattatta ttaagattat
     2881 ttgttttcga caaagaatat gttgaaggaa agaaatcata agttgagacg ataccccaac
     2941 tttcatttac ttgcattact gcatttagat aatttagttg gggtgctcat ttataataat
     3001 tttaaattta tattttcaat gaattgatga aaatcatttt atggagggcg agtatgggta
     3061 aagaatatgt agtcatcggt ctaggtcgtt ttggaggtag tatcgttcgt gagttgaatg
     3121 cattagacat ggatgtaatg gccatcgacc atgatgaaaa tagagtgaat gaatatagtg
     3181 atatcgctac tcatgcggtt gttgcagaca caacagatga agcagttatg aaaagtttag
     3241 gtatccgtaa ctttgatcat gtcattgtgg caattggtga aaatattcaa tcaagtacgt
     3301 tgacgacttt aattttaaaa gagttaggtg taaaaaaagt aactgctaaa gcacaaaatg
     3361 attatcatgc aaagatttta aataaaattg gagcagatac tgttgtgcac cctgagcgtg
     3421 atatgggtag acgtattgcg cataatgttg cgagtgcaag tgtacttgat tatcttgagt
     3481 tggcagacga gcattctatt gtagaattga aagcaactga aaagatggcg gggaagtcta
     3541 tcattgattt agatataaga gcacaatatg gaattaacat tattgcaatt aaacgaggca
     3601 aagagttcat catttcacca aatccaaata ttaatttaga aataggtgat attttaatta
     3661 tgattggaca tgataatgat ttaaatcgct ttgaaaaaaa tattgcgacg agataatcgg
     3721 gagtgggaca gaaatgatat tttctcaaaa tttatttcgt cgtcccaccc caacttacat
     3781 tgtctgtaga aattgggaat ccaatttctc tttgttgggg cccacacccc aactcgcatt
     3841 gcctgtagaa tttcttttcg aaattctcta tgttggggcc catccccaac ttgcacatta
     3901 ttgtaagctg acttttcgtc agcttctttg ttggggcccc tgactagaat tgaaaaaagc
     3961 ttgttacaaa gtgcattttc gttcagtcaa ctactgccaa tataactttg tagagcattg
     4021 aacattgatt tatgtctcaa cctcatcata tataaagaaa aagctactaa caatgtgtgg
     4081 tgagtttaat taaaacttca cttcaaagtt agtagctttt tgattattta ttgtttgatt
     4141 ctttttgctc gtttaccttc ataatgactg gtaaaatcat tggttttcta gccgtttttt
     4201 caaataaata aggttgtaat gtttcaatga tagaagattt aatctgatgc cattgaatat
     4261 ctttattttg atttaactta ctaataacgt cagttttgat tttgcgttgt gcatcataaa
     4321 ttaattgacc tgattctctc atatatacaa atcctcgaga aataatgtct ggaccagaaa
     4381 gtaatttatt tgtattaaaa tcaatactaa caacaacgat aactaaacct tcctcagata
     4441 atagcttacg gtctcttatt acaacattac cgatatcacc gataccacta ccatcaacaa
     4501 gtacattacc agatggaatg cgaccagctt tacgtgctga atcgtgtgtt aaagctaaga
     4561 catctccaat atcaaagatg aagacattat cttcttcaac gccgcattca acaccagtct
     4621 caccatgtgc ttttaacata cggtattcac catgaatagg taagaaatat ttcggcttga
     4681 ttaatcgaag cattaattgt tgatcacctt gagaaccatg ccctgaagta tggatgttag
     4741 aaatcttgct gtggataaca tctgcaccag ctttatacaa ggaattaatc gttctgttaa
     4801 tactttttgt attacctggg ataggtgatg aactaaatac aacggtatct tcaggtataa
     4861 ttttaatttg cttatgagta ccattagcaa ttctagataa tgctgccatt ggttcacctt
     4921 gagaaccagt acatagtatc aataactcat gcttcggtac ggtattaatt ttattaggtt
     4981 caataaatgt ttcaggtggt gctttaatat aaccaagttc cataccgatt ttaatattgt
     5041 tttccatcga acgaccgaac gtaacaattt tacggttatt tttgatagca gcttcaactg
     5101 cttgttgaac tcggtaaata ttagaagcga aggtagcaaa tataatacga cctttacaat
     5161 tacggaagat cttatctacg ttttgaccaa cttcacgttc gcttaaagta aaatcaggca
     5221 caagtgaatt tgttgagtct gaaagtaaac atagaacgcc ttcttcgcct aattgagcca
     5281 ttttagcaat gtttgctggt ttgcctacag gtgtaaaatc aaatttaaag tcaccggtat
     5341 gaactacttt tccttcaggt gtatctacga tgacgccata agtttcagga atactatgcg
     5401 tagttaagta gaaagaaatc gtaaagtgct tagatttaat cacactgtcc tcattgattt
     5461 catttagttt agcagtacgt aataaatgat gttcttcaag tttattacgg attaaaccta
     5521 atgctaaagg accaccataa ataggtatat taagttgttt taataggaag ggcacaccgc
     5581 ctatatggtc ttcgtgacca tgtgttataa ataggccaac aattttatct tggttttgaa
     5641 ctagatatgt gtagtcaggt ataacataat caatccctaa taagttatca tcagggaatt
     5701 tgatacctgc atcgataatg acaatttcgt ctttatactc aactgcataa gtatttttac
     5761 cgatttcacc tagacctcca agtgcatata cacctacttc atttggatgt aattgtttca
     5821 ttatttagca atctccacat taaagtgttc tgagttttct ttttcgtaat ctaaatgtgc
     5881 gccctctaat ttagtgataa attcgatatt aaaattacga tctttcaagt aacgacgtac
     5941 ttgttcttct gtttgagctt caacataaag tgattgtgta ttttcacgca caattacctc
     6001 gtctctgtta tgttgataaa aaactttaaa tactgccatg ttaaaaattc ctcctaagaa
     6061 tgtttgttta atttattgtt aaccctagta aaatcgtatt ggagtatata tcgataaatt
     6121 cattccaatc atctctataa tttaacttaa taacgatttg gttaataact aggttcatca
     6181 tgtcgttcat tttaaaaaat tagtgaaata acactaaaat ttcagttaaa tcttatttta
     6241 catgatgaat gataataaat aaagcagttt atctcactag aaatctaagt atttcgaata
     6301 aatgtgcagt tctatttctt aaaataaatg tagagattaa aaattaatgt taagaaagaa
     6361 ttaaaggatt ttatatgaaa cggtaaaatt taaatgaggg gataaaaagt ttggtagaaa
     6421 ggtgaatata tttaactgtt gtttattcga aaaagttgta caagaaaaag cttaggacat
     6481 catatttatc ctaagctttg ataattagta aaaatgtgct ttaaacttct acagcatctg
     6541 tatgtggttg taatgggtga tctttgtcaa tgtgatcata gaacattaca ccatttaaat
     6601 ggtcaatttc atgttggaaa acaattgctg gatatccttt tagacgtaat tgtatatcat
     6661 taccttcgat gtctttggct ttaattgtaa ttctattatg acggtgaact agaccagcaa
     6721 cattatcatc gacactaagg caaccttcac cagttggtaa ataagcttct tgaacgctat
     6781 gacttacaat ttttgggttc acaagcatat agtcataaga tttgccactg ccatcatctg
     6841 gtattaaaac agcaatcata cgtttagaaa tattaatttg aggtgcagcc aaaccaacgc
     6901 ctgaacgtaa accatatcgt ttcgcgattt cctcatcttg actatttact aaaaactctc
     6961 tcatggcgat taatgtttct ttttcttctt tagttaatgg taattctaac tcagctgctt
     7021 tttgacgcaa agttggatga ccatctctaa tgatgtcttt cattgttaac atatattgca
     7081 ccttccttat tttaatttgt tttagttgaa tgacagtaaa aaggttgtta agatactcat
     7141 acatttttat gtgtaaatat ctacaaagtt aaccaactac tgccaatgtt tattttagat
     7201 agtatatgta aattttcaga tatgctaatt gcttaaaaaa tgattaaaat gttgatttca
     7261 agcaatgata ctttagaaat ttatttatca tcttgacttt aaaaattata ttataaatga
     7321 cgtaactgtc aacagatata cttagtaatg aagatgtgta atgtaattgt ttaaaattga
     7381 tttccaagca gattttattt atcatttaat ttaaatagca agtggaggta caagtaatga
     7441 aatttggaaa aacaatcgca gtagtattag catctagtgt cttgcttgca ggatgtacta
     7501 cggataaaaa agaaattaag gcatatttaa agcaagtgga taaaattaaa gacgatgaag
     7561 aaccaattaa aactgttggt aagaaaattg ctgaattaga tgagaaaaag aaaaaattaa
     7621 ctgaagatgt caatagtaaa gatacaacag ttcgcggtaa agcagtaaaa gatttaatta
     7681 aaaatgctga tgatcgtcta aaggaatttg aaaaagaaga agacgcaatt aagaagtctg
     7741 aacaagactt taagaaagca aaaagtcacg ttgataacat tgataatgat gttaaacgta
     7801 aagaagtaaa acaattagat gatgtattaa aagaaaaata taagttacac agtgattacg
     7861 cgaaagcata taaaaaggct gtaaactcag agaaaacatt atttaaatat ttaaatcaaa
     7921 atgacgcgac acaacaaggt gttaacgaaa aatcaaaagc aatagaagag aactataaaa
     7981 agttaaaaga agtatcagat aagtatacga aagtactaaa taaggttggt aaagaaaagc
     8041 aagacgttga tcaatttaaa taattaatat aatacagatg gtaggaaaca actaatacag
     8101 ttcctattat ctgtatcttt ttttattaaa acagaacttt ttcaaatggt ttaacagtcc
     8161 catttatttg tggtacaatt agtaaggata aaatgaattt ctatacaatt atgggaaagg
     8221 tatggtgaat tgaatggctc ctaagttaca agcccaattc gatgcagtaa aagttttaaa
     8281 tgatactcaa tcgaaatttg aaatggttca aattttggat gagaatggta acgtcgtaaa
     8341 tgaagactta gtacctgatc ttacggatga acaattagta gaattaatgg aaagaatggt
     8401 atggactcgt atccttgatc aacgttctat ctcattaaac agacaaggac gtttaggttt
     8461 ctatgcacca actgctggtc aagaagcatc acaattagcg tcacaatacg ctttagaaaa
     8521 agaagattac attttaccgg gatacagaga tgttcctcaa attatttggc atggtttacc
     8581 attaactgaa gctttcttat tctcaagagg tcacttcaaa ggaaatcaat tccctgaagg
     8641 cgttaatgct ttgagcccac aaattattat cggtgcacaa tacattcaag ctgctggtgt
     8701 tgcatttgca cttaaaaaac gtggtaaaaa tgcagttgca atcacttaca ctggtgacgg
     8761 tggttcttca caaggtgatt tctacgaagg tattaacttt gcagcagctt ataaagcacc
     8821 tgcaattttc gttattcaaa acaataacta tgcaatttca acaccaagaa gcaagcaaac
     8881 tgctgctgaa acattagctc aaaaagcaat tgctgtaggt attcctggta tccaagttga
     8941 tggtatggat gcgttagctg tatatcaagc aactaaagaa gcacgtgacc gcgcagttgc
     9001 aggtgaaggt ccaacattaa ttgaaactat gacatatcgt tatggtcctc atacaatggc
     9061 tggtgacgat ccaactcgtt acagaacttc agacgaagat gctgaatggg agaaaaaaga
     9121 cccattagta cgtttccgta aattccttga aaacaaaggt ttatggaatg aagacaaaga
     9181 aaatgaagtt attgaacgtg caaaagctga tattaaagca gcaattaaag aggctgataa
     9241 cactgaaaaa caaactgtta cttctctaat ggaaattatg tatgaagata tgcctcaaaa
     9301 cttagcagaa caatatgaaa tttacaaaga gaaggagtcg aagtaagcca tggcacaaat
     9361 gacaatggtt caagcgatta atgatgcgct taaaactgaa cttaaaaatg accaagatgt
     9421 tttaattttt ggtgaagacg ttggtgttaa cggcggtgtt ttccgtgtta ctgaaggact
     9481 acaaaaagaa tttggtgaag atagagtatt cgatacacct ttagctgaat caggtattgg
     9541 tggtttagca atgggtcttg cagttgaagg attccgtccg gttatggaag tacaattctt
     9601 aggtttcgta ttcgaagtat ttgatgcgat tgctggacaa attgcacgta ctcgtttccg
     9661 ttcaggcggt actaaaactg cacctgtaac aattcgtagc ccatttggtg gtggcgtaca
     9721 cacacctgaa ttgcacgcag ataacttaga aggtatttta gctcaatctc caggtctaaa
     9781 ggttgttatt ccttcaggcc catacgatgc gaaaggttta ttaatttctt ctattagaag
     9841 taatgaccca gtcgtatact tagagcatat gaaattgtat cgttcattcc gtgaagaagt
     9901 acctgaagaa gaatatacaa ttgacattgg taaggctaat gtgaaaaaag aaggtaatga
     9961 catttcaatc atcacatacg gtgcaatggt tcaagaatca atgaaagctg cagaagaact
    10021 tgaaaaagat ggttattctg ttgaagtaat tgacttacgt actgttcaac caatcgatgt
    10081 tgacacaatt gtagcttcag ttgaaaaaac tggtcgtgca gttgtagttc aagaagcaca
    10141 acgtcaagct ggtgttggtg cagcagttgt agctgaatta agtgaacgtg caatcctttc
    10201 attagaagca cctattggaa gagttgcagc agcagataca atttatccat tcactcaagc
    10261 tgaaaatgtt tggttaccaa acaaaaatga catcatcgaa aaagcaaaag aaactttaga
    10321 attttaatac attttaaaag ttaacgaagt tagcgtattt tagtctcatt gattaaaatg
    10381 aaatgtttaa tttacgaaat cttaggaggg caaaaacgtg gcatttgaat ttagattacc
    10441 cgatatcggg gaaggtatcc acgaaggtga aattgtaaaa tggtttgtta aagctggaga
    10501 tactattgaa gaagacgatg ttttagctga ggtacaaaac gataaatcag tagtagaaat
    10561 cccatcacca gtatctggta ctgttgaaga agttatggta gaagaaggta cagtagctgt
    10621 agttggtgac gttattgtta aaatcgatgc acctgatgca gaagatatgc aatttaaagg
    10681 tcatgatgat gattcatcat ctaaagaaga acctgcgaaa gaggaagcgc cagcagagca
    10741 agcacctgta gctactcaaa ctgaagaagt agatgaaaac agaactgtta aagcaatgcc
    10801 ttcagtacgt aaatacgcac gtgaaaaagg tgttaacatt aaagcagttt caggatctgg
    10861 taaaaatggt cgtattacaa aagaagatgt agatgcatac ttaaatggtg gtgcaccaac
    10921 agcttcaaat gaatcagctg cttcaactac aagtgaagaa gttgctgaaa ctcctgcagc
    10981 acctgcagca gtatcattag aaggcgactt cccagaaaca actgaaaaaa tccctgctat
    11041 gcgtagagca attgcgaaag caatggttaa ctctaagcat actgcacctc atgtaacatt
    11101 aatggatgaa attgatgttc aagcattatg ggatcaccgt aagaaattta aagaaatcgc
    11161 agctgaacaa ggtactaagt taacattctt accatatgtt gttaaagcgc ttgtttctgc
    11221 attgaaaaaa tacccagcac ttaatacttc attcaatgaa gaagctggtg aaattgtgca
    11281 taaacattac tggaatatcg gtattgcggc ggacactgat agaggtttat tagtacctgt
    11341 agtgaaacat gctgaccgta aatctatttt ccaaatttct gatgaaatta atgaattagc
    11401 tgttaaagca cgtgatggta aattaacagc cgatgaaatg aaaggtgcaa catgcacaat
    11461 cagtaatatc ggttcagctg gtggacaatg gttcactcca gttatcaatc acccagaagt
    11521 agcaatctta ggaattggcc gtattgctca aaaacctatc gttaaagatg gagaaattgt
    11581 tgcagcacca gtattagcat tatcattaag ctttgaccac agacaaattg atggtgcaac
    11641 tggccaaaat gcaatgaatc acattaaacg tttattaaat aatccagaat tattattaat
    11701 ggaggggtaa aacatggtag ttggagattt cccaattgaa acagatacta tagtaatcgg
    11761 agcaggtcct ggtggatacg ttgcagcaat tcgtgcagct caattaggac aaaaagtaac
    11821 aatcgttgag aaaggtaatc ttggtggtgt ttgcttaaac gtaggatgta ttccttcaaa
    11881 agcattacta catgcttctc accgttttgt tgaagcacaa cattctgaaa acttaggtgt
    11941 tattgctgaa agtgtttctt taaacttcca aaaagttcaa gaattcaaat catcagttgt
    12001 taataaatta actggtggtg ttgaaggctt acttaaaggt aacaaagtaa acatcgttaa
    12061 aggtgaagca tatttcgtag ataacaatag cttacgtgtt atggacgaaa agagcgcaca
    12121 aacatacaac tttaaaaatg caatcattgc aacaggttca agaccaattg aaattcctaa
    12181 tttcaaattc ggtaaacgtg ttatcgactc aacaggtgct ttaaacttac aagaagtacc
    12241 aggtaaatta gttgtagttg gtggaggata cattggatca gaattaggta cagcatttgc
    12301 taactttggt tcagaagtaa caatccttga aggtgctaaa gatatcttag gtggcttcga
    12361 aaaacaaatg acacaacctg ttaaaaaagg tatgaaagaa aaaggtgttg aaatcgttac
    12421 tgaagctatg gctaaatctg ctgaagaaac agataacgga gttaaagtta cttatgaagc
    12481 taaaggcgaa gagaaaacaa tcgaagctga ttatgtatta gtaactgtag gtcgtcgtcc
    12541 aaacacagac gaattaggcc tagaagaatt aggtgttaaa ttcgctgacc gtggattatt
    12601 agaagttgat aaacaaagcc gtacgtctat cagcaatatc tatgcaattg gtgatatcgt
    12661 tccaggttta ccacttgctc acaaagctag ctatgaagct aaagttgctg ctgaagcaat
    12721 tgatggtcaa gctgctgaag ttgattacat tggtatgcca gcagtatgct ttactgaacc
    12781 agaattagct acagttggtt attcagaagc gcaagctaaa gaagaaggtt tagcaattaa
    12841 agcttctaaa ttcccatatg cagcaaatgg tcgtgcatta tcattagatg atactaacgg
    12901 atttgttaaa cttattacac ttaaagaaga tgatacttta atcggtgctc aagtagttgg
    12961 tactggtgca tcagatatta tctctgaatt aggtttagca attgaagctg gtatgaatgc
    13021 tgaagatatc gcattaacaa tccatgcaca tccaacatta ggtgagatga ctatggaagc
    13081 agcagaaaaa gctatcggat acccaatcca tacaatgtaa taactgatta tctataaaga
    13141 ttcagtcatt aaaagctgta gcatatgcta cggctttttt gttttaggta aagtaatgta
    13201 aggaaattga tttgagatat cgttaacatg tgacatgcat gttatactag cgatgctaac
    13261 aaaagaattg aaatggaggg ttcaacaatg gaatatgagt atccaattga tttagactgg
    13321 agtaatgaag agatgatttc agtgataaat ttctttaatc atgtagagaa gtattatgaa
    13381 tccggcgtga cggcaggcga ctttatgggt gcatataaaa gatttaaaga aattgtgcct
    13441 gctaaagcag aggaaaaaca aatttttaat actttcgaaa aaagtagtgg ctataatagt
    13501 tacaaagcag ttcaagatgt aaaaactcac tctgaagaac aaagagtaac agctaaaaaa
    13561 taattcgttc gaaattaaca caatttaata ggaatttttc tttaaaacta ttgctaataa
    13621 agctatattt tgataccttt atcaagtgtt aaacaaaatg tttgataaaa gtaaacttaa
    13681 tatagctttt ttaggtggaa aaataaatga acataggtaa taaaattaaa aatcttagaa
    13741 gaattaaaaa tttaacgcaa gaagaacttg ctgaacgtac agacttatcg aaaggctaca
    13801 tttcacaaat agaaagtgaa catgcctcac caagtatgga aactttctta aatattatag
    13861 aggtgttagg aacgacgcca agtgaatttt ttaaagacag tgaaaatgaa aaagtattat
    13921 acaagaagga agaacaagtt atttatgatg agtatgatga aggttatata ttaaattggt
    13981 tagtttcaaa gtcaaatgaa tatgatatgg agccattaat attaacttta aagcctggcg
    14041 catcatataa aaattttaat ccatcagagt ctgatacgtt tatttattgt atgtcaggtc
    14101 agataacact taatttaggc aaagaggtat atcaagcaca agaagaagac gttttgtatt
    14161 ttaaagcacg agataatcat cgtttgtcaa atgaatcaaa caatgaaaca cgaatactta
    14221 ttgtagcgac agcttcatat ttataggggg gatcttattt ggaaccgtta ttatcattaa
    14281 aatcagttag taaaagctat gatgatctta atatcttaga tgacatagat attgatattg
    14341 aatcaggata cttttataca ttattaggtc cttcaggttg tggtaaaaca acaattttaa
    14401 aattaattgc agggtttgaa tatcctgaca gtggtgaagt gatttatcaa aacaaaccaa
    14461 ttggtaattt accaccaaat aaacgtaaag tgaatacagt ctttcaagat tatgcattat
    14521 ttccacactt aaacgtctat gataatatcg ctttcggttt gaaattaaaa aaattatcaa
    14581 aaaccgaaat tgatcaaaaa gtaactgagg cattaaaatt agttaagctt tcaggttatg
    14641 aaaaaagaaa tattaatgaa atgagtggcg gacaaaagca acgtgttgca attgcacgtg
    14701 ctatcgtaaa tgaaccagaa atattattat tagatgaatc tttatccgca ttagatttga
    14761 aattgcgcac tgaaatgcaa tatgaattac gggaattgca atctagatta ggtattacat
    14821 ttatatttgt aacacatgat caagaagaag cgttagcact aagtgacttt ctttttgtat
    14881 taaaagatgg gaaaattcaa caatttggta cgccaacgga tatatatgac gaaccagtga
    14941 atcgatttgt agctgatttt attggagaat ctaatattgt tgaaggacgc atggttagag
    15001 attatgtcgt gaatatttat gggcaagatt tcgaatgtgt cgatatgggt attcctgaaa
    15061 ataaaaaagt agaagtcgtt attcgaccag aagatatatc attaatcaaa gctgaagaag
    15121 gattatttaa agcaactgtt gattctatgt tatttagagg ggtccactat gaaatatgtt
    15181 gtatagacaa taaaggttat gaatgggtaa tacaaacgac taaaaaagct gaagtaggca
    15241 gtgaagttgg tctttatttt gatcctgaag ccattcatat tatggttcct ggagaaacag
    15301 aagaagagtt tgataaacgt attgaaagct atgaggaagt agacaatgcg taatactaat
    15361 aaatttctct taatcccgta tttattatgg atggttatat ttattattgt accagttgta
    15421 ttactcattt atttttcatt tttagatatc aatggacatt ttagtttcac gaattatcaa
    15481 caaattttta ctacaaaata tttaaaaatg tttgcatatt caattttata tgtcgcttta
    15541 ataacaatta ttacgttggc tataagttat ccagctg
//
LOCUS       DAHHOJ010000064        15524 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.064, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000064 DAHHOJ010000000
VERSION     DAHHOJ010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15524
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.064"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(149..1441)
                     /gene="asnS"
                     /locus_tag="NBH32_001679"
     CDS             complement(149..1441)
                     /gene="asnS"
                     /locus_tag="NBH32_001679"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCD1515272.1"
                     /translation="MKTTIKQAKDHLNQDVTIGAWLTNKRSSGKIAFLQLRDGTGFMQ
                     GVVVKSEVDEEVFKLAKEITQESSLYVTGTITEDNRSDLGYEMQVKSIEVISEAHDYP
                     ITPKNHGTEFLMDHRHLWLRSKKQHAVMKIRNEVIRATYEFFNKDGFTKVDPPILTAS
                     APEGTSELFHTKYFDQDAFLSQSGQLYLEAAAMAHGKVFSFGPTFRAEKSKTRRHLIE
                     FWMIEGEMAFTNHAESLEIQEQYVTHVVKSVLENCKLELKMLERDTSKLEKVATPFPR
                     ISYDDAIEFLKAEGFDDIEWGEDFGAPHETAIANHYDLPVFITNYPTKIKPFYMQPNP
                     ENEETVLCADLIAPEGYGEIIGGSERVDDLELLEQRVKEHGLDEEAYSYYLDLRRYGS
                     VPHCGFGLGLERTVAWISGVEHVRETAPFPRLLNRLYP"
     gene            complement(1763..4456)
                     /locus_tag="NBH32_001680"
     CDS             complement(1763..4456)
                     /locus_tag="NBH32_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCD1515273.1"
                     /translation="MGMATYAVVDLETTGNQLDFDDIIQIGITFVRNNQIIDTYHSMI
                     RTNLEIPPFIQALTSIEENMLQQAPYFNQVAQEIYDKIKDCIFVAHNVDFDLNFIKKA
                     FKDCNIQYRPKKVIDTLEIFKIAFPTDKSYQLSELAEAHGINLANAHRADEDAATTAK
                     LMILAFEKFEKLPLDTLKQLYYLSKQLKYDLYDIFFEMVRQYDAKPLDKSYEKFEQII
                     YRKQVDFKKPTTNYNGSLKSLYSKAVDQLGLTYRPQQLYLAETILDQLMHSEKAMIEA
                     SLGSGKSLAYLLAALMYNIETGKHVMISTNTKLLQSQLLEKDIPAMNEALNFKINALL
                     IKSKSDYISLGLISQILKDDTSNYEVNILKMQLLIWITETPSGDIQELNLKGGQKMYF
                     DQKIETYVPARHDVHYYNFIKRNAQNIQIGITNHAHLIHSDVENSIYQLFDDCIVDEA
                     HRLPDYALNQVTNELSYADIKYQLGLIGKNENEKLLKAIDQLEKQRILEKLDIAPIDI
                     FGLKASMNEIHELNEQLFSTIFTIINDSDVYDDDIHRFHNVFTFETKDILKDLHAIID
                     KLNKTLEIFNGISHKTVKSLRKQLLYLKDKFKNIEQSLKAGHTSFISIKNLSQKSTIR
                     LYVKDYAVKDVLTKQVLEKFKSLIFISGTLKFNHSFEAFKQLFNKDVHFNTFEVNTSL
                     QSAKNTSVFIPSDVASYQYKNIDEYVASIVSYIIEYTTITSSKCLVLFTSYKMMHMVQ
                     DMLNELPEFEDYVVLTQQQNQNYKIVQQFNNFDKAILLGTSTFFEGFDFQANGIKCVM
                     IAKLPFMNKHNAKYWLMDSEFTSTFKEYVLPDAVTRFRQGLGRLIRNENDRGIIVSFD
                     DRLINSNYKNFFEQTLENYRQKKGDIQQFGKLLRQIQKKKK"
     gene            complement(4480..5451)
                     /locus_tag="NBH32_001681"
     CDS             complement(4480..5451)
                     /locus_tag="NBH32_001681"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HCD1515274.1"
                     /translation="MSKYSQDVLQLLYKNKPNYISGQSIAESLNISRTAVKKVIDQLK
                     LEGCKIDSVNHKGHLLQQLPDIWYQGIIDQYTKSSALFDFSEVYDSIDSTQLAAKKSL
                     VGNQSSFFILSDEQTKGRGRFNRHWSSSKGQGLWMSVVLRPNVAFSMISKFNLFIALG
                     IRDAIQHFSQDEVKVKWPNDIYIDNGKVCGFLTEMVANNDGIEAIICGIGINLTQQLD
                     DFDESIRHRATSIQLNDKNKLDRYQFLERLLQEIEKRYNQFLTLPFSEIREEYIAASN
                     IWNRTLLFTENDKQFKGQAIDLDYDGYLIVRDEAGESHRLISADIDF"
     gene            complement(5438..6640)
                     /locus_tag="NBH32_001682"
     CDS             complement(5438..6640)
                     /locus_tag="NBH32_001682"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HCD1515275.1"
                     /translation="MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDI
                     TTSATPDEIESIFSHTIPVGKEHGTINVVFNDENYEVTTFRAEEDYVDHRRPSGVTFV
                     RDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAEERFQEDALRMI
                     RCLRFQSQLSFDIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEESFNHLKS
                     LKAFNYMPYFEHLDMNQINVTEAIDLELLIAIASVKFDINYSLKPLKLSNRQVKDINQ
                     YIQIMNALPSIITKEQLKMFVYDYDTNLIKNVMVAADVLKANDIQGHEPLIVNLQTID
                     ETLHRLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILK
                     WVDNHVKI"
     gene            complement(6645..7787)
                     /gene="bshA"
                     /locus_tag="NBH32_001683"
     CDS             complement(6645..7787)
                     /gene="bshA"
                     /locus_tag="NBH32_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-alpha-D-glucosaminyl L-malate synthase
                     BshA"
                     /protein_id="HCD1515276.1"
                     /translation="MKIGITCYPSMGGSGIIATELGIKLAERGHEVHFITSNIPFRIR
                     KPLPNMIFHQVEVNQYAVFQYPPYDITLSTKIAEVIKEYDLDLLHMHYAVPHAICGIL
                     AREMSGKDIKIMTTLHGTDITVLGYDHSLQGAIKFGIEKSDIVTSVSKSLAQETHEII
                     ETNKEIIPIYNFVRENEFPTKHNTALKSQFGIAPDEKVLIHVSNFRQVKRIDTIIETF
                     AKVREKIPSKLILLGDGPELVPMRQLTKELNVEEDVLFLGKQDCVSEFYQLSDLVLLL
                     SEKESFGLTLLEAMKTGVVPIGSNAGGIKEVIKHGETGFVVDVGDCDSASDYAIRLLE
                     DKALYNKLQKNMLADIAERFGSELITDQYEYYYQKMLNEHNKSKGE"
     gene            complement(8025..8342)
                     /locus_tag="NBH32_001684"
     CDS             complement(8025..8342)
                     /locus_tag="NBH32_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HCD1515277.1"
                     /translation="MKSMVEMQREVDEYIGQFKTGYFSPLANLARLTEEVGELAREIN
                     HTYGEKKKKDSEEANTIKAELGDNLFVLLCLANSMGIDMTESFNETMEKFNTRDKNRF
                     ERK"
     gene            complement(8679..9359)
                     /locus_tag="NBH32_001685"
     CDS             complement(8679..9359)
                     /locus_tag="NBH32_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HCD1515278.1"
                     /translation="MIIYFVILMVLPLWAQHKVKSNYEKYSQVRSTSGKTGREVALEI
                     LHANGIYDVEVVKGEGFLTDHYDPKKKVVSLSPANYDRPSVAGTAIAAHEVGHAIQDH
                     QGYWFLRFRAALVPVANLGSSLSYMIIMLGIILTAIGSAFGSTALWIGAGLMSLAVLF
                     SIVTLPVEFDASSRAMKQITALNIVNEKEYKHARKVLSAAAMTYVAATAVAVAELVRI
                     ILIARSSD"
     gene            complement(9431..10018)
                     /locus_tag="NBH32_001686"
     CDS             complement(9431..10018)
                     /locus_tag="NBH32_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1405 domain-containing protein"
                     /protein_id="HCD1515279.1"
                     /translation="MTINTFWQYTLYQRSWLMMLLICNILGMIYGYIWYGEQLSHTPW
                     HFKIFVPDSPTAILYLVISISLILIQKQNSIIDALAFVTLFKYGIWAVIMNILFIIEQ
                     GDITVNGLVLMFSHSIMAVQAIYFYPRFKRSMIGISVAMIWVFLNDYIDYFHLQFPYY
                     DFITTHVWQIGVLSCCLSVFGLLLYIELNKLLKCK"
     gene            complement(10008..10583)
                     /locus_tag="NBH32_001687"
     CDS             complement(10008..10583)
                     /locus_tag="NBH32_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HCD1515280.1"
                     /translation="MSETLNQIKESFIEYLLFQYRFKSRIAVWVLNYIKVNEAKLANT
                     HFVDTKINHHETLEIAEVGSHASAIQFTKRNIKLMNTNEIFDYIANHNCAFDIQIHFA
                     NVSKREQRLDDLIVAQLTESPSYQTYLHDLNSMAIDRHKHALLIDYLLHNIDLSLQMN
                     EKQRFYQLTQILNTLKLVNKHNQFEDLADDD"
     gene            complement(10597..11841)
                     /locus_tag="NBH32_001688"
     CDS             complement(10597..11841)
                     /locus_tag="NBH32_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000389522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515281.1"
                     /translation="MEDIYKLIDDINLQKLENLDSRVNEAITTDNDDALFILGETLYN
                     FGLMPQGLEVFRVLYHKYPDESELLIYFIEGLMSENQTDEALEYLSYVEPSPEKLMLE
                     ADLYQQINMMEVAIDKLQEALELEPNDPIIHFALAEMLYYDGQYLRATSEYETVLETG
                     EYQVNGVNLFSRMADCSLQSGNYSDAIRLYDEINEDEMTSEDYLKKAISYDKNDITQE
                     AIKIMTTLLSKDPDYIQGYLYLQSLYENEKNYPDAIETGKEGLRLSQFYKELMYTTGC
                     LEIEHGDANEGVLLLKQALEVDNAYQEPLLILSDLYRNEEDYEAIIELLTYVDEGDLD
                     PTFMWHLAFAYGQEERDKEAQHFFELAYPTMKTNIDFMSDYYFYLIEIGQKEQAITIL
                     NQLLELEPSNENWHDELSRLQS"
     gene            complement(11848..13146)
                     /gene="aroA"
                     /locus_tag="NBH32_001689"
     CDS             complement(11848..13146)
                     /gene="aroA"
                     /locus_tag="NBH32_001689"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCD1515282.1"
                     /translation="MVNEQIIDISGPLKGEIEVPGDKSMTHRAIMLASLAEGVSTIYK
                     PLLGEDCRRTMDIFRLLGVEIKEDDEKLVVTSPGYQSFNTPHQVLYTGNSGTTTRLLA
                     GLLSGLGIESVLSGDVSIGKRPMDRVLRPLKSMNANIEGIEDNYTPLIIKPSVIKGIN
                     YKMEVASAQVKSAILFASLFSKEPTIIKELDVSRNHTETMFRHFNIPIEAEGLSITTT
                     PEAIRYIKPADFHVPGDISSAAFFIVAALITPGSDVTIHNVGINPTRSGIIDIVEKMG
                     GNIQLFNQTTGAEPTASIRIQYTPMLQPITIEGELVPKAIDELPVIALLCTQAVGTST
                     IKDAEELKVKETNRIDTTADMLNLLGFELQPTNDGLIIHPSEFKTNATVDSLTDHRIG
                     MMLAVASLLSSEPVKIKQFDAVNVSFPGFLPKLKLLENEG"
     gene            complement(13156..14220)
                     /locus_tag="NBH32_001690"
     CDS             complement(13156..14220)
                     /locus_tag="NBH32_001690"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCD1515283.1"
                     /translation="MKLQTTYPSNNYPIFVEHGAIDHISTYIDQFDQSFILIDEHVNQ
                     YFADKFNDILSFGNVHKVIIPAGEKTKTFEQYQETLEYILSHHVTRNTAIIAVGGGAT
                     GDFAGFIAATLLRGVHFIQVPTTILAHDSSVGGKVGINSKQGKNLIGAFYRPDAVIYD
                     LDFLNTLPFDEILSGYAEVYKHALLNGKSATHDIEQHFKDRASLQSLNGIDKYITKGI
                     ETKLNIVVADEKEQGVRKYLNLGHTFGHAVEYNHKIAHGHAVMIGIIYQFIVANILFN
                     SNHDIQHYINYLTKLGYPLETITDIDFETIYQYMLSDKKNDKQGVQMVLIKHFGDIVV
                     QHVDQLTLQHACEQLKTYFK"
     gene            complement(14246..15412)
                     /gene="aroC"
                     /locus_tag="NBH32_001691"
     CDS             complement(14246..15412)
                     /gene="aroC"
                     /locus_tag="NBH32_001691"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCD1515284.1"
                     /translation="MRYLTSGESHGPQLTVIVEGIPANLEIKVEDINKEMFKRQGGYG
                     RGRRMQIEKDTVEIVSGVRNGYTLGSPITMVVTNDDFTHWRKIMGAAPISDEERENMK
                     RTITKPRPGHADLVGGMKYNHRDLRNVLERSSARETAARVAVGALCKVLLEQLDIEIY
                     SRVVEIGGIKDKDFYDSETFKANLDRNDVRVIDDGIAQAMRDKIDEAKNDGDSIGGVV
                     QVVVENMPVGVGSYVHYDRKLDGRIAQGVVSINAFKGVSFGEGFKAAEKPGSEIQDEI
                     LYNTELGYYRGSNHLGGLEGGMSNGMPIIVNGVMKPIPTLYKPLNSVDINTKEDFKAT
                     IERSDSCAVPAASIVCEHVVAFEIAKALLEEFQSNHIEQLKQQIIERRQLNIEF"
BASE COUNT         4896 a         2861 c         2116 g         5651 t
ORIGIN      
        1 aatatagtta ctaaacaaaa gtttgttcat gattaatata agccgttatc aatcttcatt
       61 ttccaagcat aaatcgccca gctattaata taggctggac gattcttttt attatcataa
      121 tataaatatt aagtaacgat cctaaaactt atggatataa acggtttaat aatcttggga
      181 atggcgcagt ttcacgaacg tgttcaacac cagaaatcca tgctactgta cgctctaaac
      241 ctaaaccaaa tccacagtgt ggcacactac cataacgacg taagtctaag tagtaactat
      301 atgcttcttc gtctaatcca tgttctttaa cgcgttgttc taacaattct aagtcatcca
      361 cacgttcaga tccacctata atttcaccgt atccttcagg tgcaattaag tctgcacata
      421 atacagtttc ttcattttca ggatttggtt gcatatagaa aggcttaatt ttagttggat
      481 aattagtaat aaacaccggt aaatcataat gattagcaat ggctgtttca tgtggcgcac
      541 caaaatcttc gccccattca atatcatcaa agccttctgc ttttaagaat tcaattgcat
      601 catcatatga aattctaggg aatggtgtcg caactttttc aagttttgat gtatcacgct
      661 ctaacatttt caactctagt ttacaatttt ctaaaactga ttttacaaca tgtgttacat
      721 attgttcttg aatttctaaa ctttcagcat gatttgtgaa agccatttcc ccttcaatca
      781 tccagaactc gatcaagtgt ctacgtgttt ttgatttttc agctctgaaa gttggaccaa
      841 atgaaaatac ttttccgtgt gccatagctg cagcttctaa gtataactga ccactttgag
      901 ataaaaacgc atcttgatca aagtatttag tatggaataa ttcacttgta ccttctggcg
      961 cacttgctgt caaaattggt ggatcaacct ttgtaaatcc atctttgttg aaaaattcat
     1021 acgttgcacg aataacttca tttctaattt tcattacagc atgttgtttt ttagaacgta
     1081 accataaatg acggtgatcc attaagaatt ctgtaccatg atttttaggt gtaatcggat
     1141 agtcatgcgc ttctgaaata acttcaattg atttcacttg catttcgtat cctaagtcag
     1201 aacgattatc ttctgtaatt gtgcctgtaa catatagaga tgattcttga gtaatttctt
     1261 tcgcaagttt gaatacctct tcatcaactt ctgatttaac tactacgcct tgcataaagc
     1321 ctgttccatc acgtaattgt aaaaaggcga ttttaccact tgaacgttta tttgttagcc
     1381 aagcaccaat tgtaacgtct tggtttaaat gatctttcgc ttgtttaatc gttgttttca
     1441 taaccattct cctatttatt ttttcgttat acaatactca ttcattttaa caaaatccgc
     1501 tttcaagttc tagaactaga ctaaaagata acgtgtaaat ggtaatgatt taagcacaga
     1561 tttgaacatt aatttatatt aagacaatac aatcatcagt ttcagacaaa atatataata
     1621 aatcctcaat attattagta tgaaggtcgt tttttgtgct aacattcaag tttaattata
     1681 aaactcaaat tttgacgacc aaacaaaaat tcttgtaaca gaacatagca catgtcatgt
     1741 cacaagaatt ttactttgta tttcactttt tctttttttg aatttgtctt aataattttc
     1801 caaactgttg aatgtcgcct tttttctgac gataattttc aagtgtttgt tcaaaaaagt
     1861 ttttataatt actgtttatg agtcgatcat caaatgaaac tattatgccg cgatcatttt
     1921 catttctaat taatcttcca agtccttgtc taaaacgtgt aactgcatca ggtaatacat
     1981 attccttgaa agttgaagtg aattcagaat ccataagcca atattttgca ttatgcttgt
     2041 tcataaacgg taacttcgct atcatcacac atttaatacc atttgcttga aaatcaaaac
     2101 cttcaaaaaa tgttgacgta ccaagcagta tggccttatc aaaattatta aactgttgta
     2161 ctattttata attttggttc tgctgttgtg ttaatacaac ataatcttca aattctggca
     2221 attcatttag catatcttgt accatatgca tcattttata actcgtaaat aagactaaac
     2281 attttgatga cgttatagtc gtatattcaa taatatagct tacaatcgat gctacatact
     2341 catctatatt tttatattga taagatgcta catcactcgg tataaataca cttgtatttt
     2401 ttgcactttg taacgacgtg ttaacttcaa atgtattaaa gtgaacatct ttgttgaata
     2461 actgtttaaa agcttcaaac gaatgattaa attttaaagt accagatata aaaatgagtg
     2521 atttaaattt ttccaatact tgtttcgtta atacatcttt tacagcgtaa tctttcacat
     2581 ataaacgtat tgttgatttt tgagataaat ttttaattga aatgaaacta gtatgtccag
     2641 cttttaaact ttgttcgata tttttaaatt tatcttttaa atacaacaac tgtttccgta
     2701 atgatttaac tgttttatga ctaatgccat tgaatatttc tagcgtttta tttaacttat
     2761 cgataatcgc atgtaaatcc ttcaaaatgt cttttgtttc aaaagtaaat acattatgga
     2821 agcgatgaat atcatcatca taaacatcag aatcattgat aatcgtaaat atcgttgaaa
     2881 acaattgctc atttaactca tgaatctcat tcatactagc cttcaagcca aaaatatcaa
     2941 ttggtgcaat atctaacttt tccaaaattc gctgcttttc cagttgatca attgccttta
     3001 acaatttttc attttcgttt ttaccaatca aaccaagctg atatttaata tcagcataac
     3061 tcaactcatt tgtcacttga tttaaggcat agtctggtaa gcgatgtgct tcatccacta
     3121 tacaatcatc aaacaattga tatattgaat tttcaacatc agaatgaatt aaatgtgcat
     3181 gatttgtaat accaatttga atgttctgtg catttcgctt aataaaatta taataatgga
     3241 catcgtgacg tgccggtaca tatgtttcaa ttttctggtc aaaatacatc ttttgaccac
     3301 cttttaaatt taattcctgt atatctccgg acggcgtctc tgtaatccaa atcagcaatt
     3361 gcattttcaa gatattcact tcgtagttac ttgtgtcatc ttttaaaatt tgactaataa
     3421 gccccaatga aatgtaatca cttttacttt taatcaatag tgcattaatt ttaaaattta
     3481 acgcttcatt cattgctgga atatcttttt ctaacaattg actttgcagt aatttggtat
     3541 tggtagaaat cataacatgc tttccagttt caatattata catcaaggcc gcaagtaaat
     3601 atgctaatga tttaccactg cctagtgatg cttcaatcat tgctttttca ctatgcatga
     3661 gctgatctaa tatagtttcc gctaaatata attgctgcgg tcgatatgtt aagccaagtt
     3721 gatctacagc tttgctatat aaagacttca agctgccatt ataatttgtt gtcggctttt
     3781 taaaatcaac ttgcttacga tagataatct gttcgaactt ttcgtacgat ttatccaatg
     3841 gctttgcatc atattgccta accatctcaa agaaaatatc atacaaatcg tatttcaact
     3901 gtttacttaa ataatataat tgctttaaag tatctaacgg taacttttca aatttttcaa
     3961 aagctaatat catcaattta gcagtagtag cggcatcctc gtcagctcga tgggcatttg
     4021 ctaagttaat accatgtgct tctgctaatt cacttaattg ataactttta tctgtaggaa
     4081 aagctatttt aaagatttct agtgtatcta taactttttt gggacgatat tgaatattac
     4141 aatctttaaa tgccttttta ataaaattta aatcaaaatc tacattatga gcgacaaaaa
     4201 tgcaatcttt tatcttatcg tagatttctt gtgcaacttg attaaaatat ggcgcttgtt
     4261 gtagcatatt ttcttcaatg gatgttaacg cttgaatgaa tggcggaatc tctaaatttg
     4321 ttctaatcat agaatgatat gtatcaataa tttggttatt gcgcacaaac gttataccaa
     4381 tttgaatgat atcgtcaaaa tctaattggt tgcctgttgt ttccaaatcc acaacggcat
     4441 aggttgccat acccatagct atctctcctt gctttagtgt taaaaatcta tatctgcact
     4501 aattaaacgg tgtgattcac ccgcttcatc tctaacaatt agatagccat cgtaatctaa
     4561 atcaattgct tgtcctttaa actgtttatc attttctgta aatagcaacg ttctattcca
     4621 aatattagaa gctgcaatat attcttcacg aatttcagaa aaaggtaacg ttaaaaattg
     4681 attatatctt ttttcaattt cttgaagtaa tctctctaaa aattgatatc tatctaattt
     4741 atttttatca tttaattgta tacttgttgc tctatgtcta atactttcat caaagtcatc
     4801 tagttgttgc gtcaaattaa tacctatacc acatattatt gcttctatac catcattatt
     4861 agcaaccatt tcagttaaga aaccacacac tttaccatta tcaatatata tatcattcgg
     4921 ccatttcact ttgacttcat cttgactaaa atgttgaatc gcatctctta tccctaatgc
     4981 aataaataaa ttaaatttag atatcattga gaatgcaacg ttaggtctta acacgacaga
     5041 catccaaagt ccttgccctt ttgaagaact ccaatgtcta ttaaatcgcc cacgaccttt
     5101 cgtttgttca tcactcaaga taaaaaatga agattgattt ccaacaagtg actttttcgc
     5161 agcaagttgt gtagaatcta ttgaatcgta tacttcacta aaatcaaaca aagcagaact
     5221 ttttgtatat tggtctatta taccttgata ccaaatatct gggagctgtt gtaataaatg
     5281 ccctttatga tttactgaat ctattttaca tccctctaat tttaattggt caatcacttt
     5341 ttttactgca gtgcgcgata tattaagtga ttccgcaatg ctttgtccag atatataatt
     5401 cggtttattt ttatagagta attgaagtac atcttgacta tattttgaca tgattatcca
     5461 cccatttcaa aatttcagtt tcttcgttgc ttactttacc tgttacaatc gctatctcaa
     5521 tttgtcttag cacatctttt aaccatggac cacttttggc atttaaatgt gccataagta
     5581 caccgccatt aaccatcatg tcttttctat tatgcatagg taaacgatgt aatgtttcat
     5641 caatcgtttg aaggttaacg attaatggtt catgtccttg aatatcattt gcctttaaca
     5701 cgtctgctgc aaccattaca tttttaatga gattcgtatc ataatcataa acaaacattt
     5761 ttaattgttc ttttgtaata atacttggta atgcattcat aatttgaata tattgattga
     5821 tatcttttac ttgtcggtta cttagcttta aaggcttcaa tgagtaatta atatcaaatt
     5881 taactgatgc tatagcaatc aataattcta aatcaattgc ttcagtcaca ttaatttgat
     5941 tcatatcaag atgttcgaaa tatggcatat aattaaatgc tttcagcgat tttaaatgat
     6001 taaaactctc ttcaacatta atacctcgca ttaatttagt tagttcaatc actatacgct
     6061 caattgataa aaatttaata tctgccattt gtgtacgcat cgcttcgaat gtttccgttg
     6121 caatatcaaa tgataattgt gactggaatc ttaaacatcg aatcatacgt aaagcatctt
     6181 cttggaatcg ttcctcagct atacctacag ttcttattat tcgattatta atatcttgtt
     6241 gaccatcaaa ataatcatac aatttgtatg ctgtatccat tgctatcgca ttcatcgtga
     6301 aatctcttcg ttgcaaatct tcgtataaat cacgaacaaa tgtaacacca cttggtctac
     6361 ggtgatcgac ataatcttct tcagcccgga atgttgtcac ttcataattt tcatcattaa
     6421 aaactacatt tatcgtgcca tgttctttac ctacaggtat cgtatgacta aagattgatt
     6481 ctatttcatc cggcgttgca cttgttgtga tatctatatc atgaatattt cttcccatga
     6541 cataatctct tacagagcca cctacataat atgcttcaaa accattgtct tgaatttgtt
     6601 ctaatatagg ccttgcctgt tcaaataatg atttatccat attattactc gcctttactt
     6661 ttgttatgtt catttagcat tttttgataa taatactcat attgatctgt aatgagttct
     6721 gatccaaaac gttcagcaat atctgctagc atgtttttct gaagtttgtt gtataacgcc
     6781 ttatcttcaa gtaatcggat agcatagtca ctcgctgaat cacaatcacc cacatctaca
     6841 acaaatccag tttcaccatg tttaataacc tctttaattc caccggcatt tgaaccaatt
     6901 ggaacgacgc ctgttttcat agcctcaagt aaagttagtc caaagctttc tttttcactt
     6961 aataataata ctaagtcaga taattgataa aattcactta cgcaatcttg cttccctaaa
     7021 aataaaacat cctcttctac gtttaactct ttcgtcaatt gacgcattgg cactaattca
     7081 ggaccatctc caagtaaaat taatttacta ggtatctttt cacgtacttt tgcaaatgtt
     7141 tctataatag tatctatgcg ttttacttgt ctaaaattcg atacatgtat taacactttt
     7201 tcatctggtg ctataccaaa ttgtgatttt agtgctgtgt tatgtttagt tggaaactca
     7261 ttttcacgta caaaattata aatcggtata atttctttgt tagtttcgat aatttcatgt
     7321 gtttcttgcg ctaaagattt actcacactt gtcacaatat cacttttttc aatgccaaat
     7381 ttaattgcac cttggagtga atgatcatag cccaaaacag taatatcagt accgtgtagc
     7441 gttgtcataa tttttatatc tttacctgac atctcacgag ctaaaatacc acaaattgca
     7501 tgaggtacag catagtgcat atgcaacaaa tcaagatcat attctttaat aacttcagcg
     7561 attttagtac ttaacgtaat atcatacggt ggatattgaa atactgcata ttgattcact
     7621 tcaacttgat gaaaaatcat attcggtaat ggttttctta ttctaaacgg gatatttgaa
     7681 gtgataaaat gtacttcgtg acctcgctct gctaatttaa ttcctaattc tgtggcaata
     7741 attccagaac cacccatgga cgggtaacat gttataccta tcttcattcg cttgcccatc
     7801 ctttctttct atttctatta ttctcgatgc gtagataatt gtttaaattt aagtttatag
     7861 taatgttgag tttataattt caaatatcta aaaaacaggt gttgtatata taatcattca
     7921 tctagttata cttactttaa aaataatatg atttcatgcg atgcaattca ttgatggatg
     7981 tttttaatct taatcaaatc caaataaagc atatattttt atattcactt tctttcgaat
     8041 cgatttttat ctcttgtatt aaacttttcc atagtttcat taaagctctc tgtcatatct
     8101 attcccattg aattcgctaa acataacaac acaaataaat tatcacctaa ttctgcttta
     8161 atcgtatttg cttcctctga atctttcttc tttttttcac cataggtatg atttatttca
     8221 cgtgcaagtt cgcccacttc ttcagtcaat ctagctaagt tagctaatgg tgaaaaatat
     8281 cctgttttaa attgtccaat atattcatca acttcacgtt gcatttctac cattgatttc
     8341 atttctacgt tctccttata ttgcatttct aatatagtat atatcaattt gaagtctcat
     8401 gcatgtttag tgcaatctac tgactctagc taaactagat gacaagttgt ttattttaga
     8461 agtttaataa ttcagtttat ataaatgtaa tgcattccta actaaattaa atcaattgaa
     8521 attgggatta taactttatg atacgtacta ctacaataaa ataatatagt gaataatcta
     8581 ccatgagaaa aataagcaca aaaaaactag caaccacaca aaaatgtgat tagctagtta
     8641 ataagtgtct aatttaagtt aattgttaat ctataagatt aatcacttga acgcgcaatc
     8701 aaaataatac gtacaagctc tgctacagcg acagcagttg ctgcaacata agtcattgct
     8761 gctgcagata atactttacg cgcatgcttg tattcttttt catttacaat gttcaatgcc
     8821 gtaatttgtt tcatcgctct tgaactcgca tcaaactcaa ctggtaacgt aacaattgag
     8881 aataataccg ctaatgacat taaaccagca ccaatccata aagcagttga accaaatgca
     8941 ctacctatcg ctgttaagat aatacctaac atgatgatca tataacttaa tgaactccct
     9001 aggtttgcaa caggtactaa tgctgctctg aatcttaaga accaatatcc ttggtgatct
     9061 tgaatggcat gaccaacttc gtgggctgca attgcagttc cagcaactga tggtctgtca
     9121 tagtttgcag gagatagtga aacaactttc tttttaggat cgtaatgatc tgttaagaat
     9181 ccttcacctt taacaacttc gacatcataa ataccgtttg catgtaaaat ttctaatgca
     9241 acttcacgac ccgttttacc actagttgat ctaacttgtg aatatttctc atagttagat
     9301 ttaactttgt gttgtgccca taaaggaagc accattaata ttacgaaata aattatcata
     9361 gtaaaaattg aagacaataa actcactctc ctttataaat attttactgt catttgccgt
     9421 ttttatcaaa tcatttacac tttaataatt tgtttaattc aatataaagc aaaagtccaa
     9481 aaacacttag acaacatgat aatacaccaa tttgccacac atgtgtagtt ataaaatcat
     9541 aatatggaaa ttgaaggtga aaatagtcaa tataatcatt caaaaatacc caaatcatcg
     9601 ctacactgat tccaatcata gaacgtttaa acctaggata gaagtaaatt gcctgaacag
     9661 ccattatact gtgggaaaac attaatacca aaccatttac tgtaatatca ccttgttcaa
     9721 taataaataa tatattcatt ataactgccc aaatcccata tttgaataat gttacaaatg
     9781 ccagtgcatc gataatacta ttttgttttt gaattaatat caatgagata gaaataacta
     9841 agtataatat tgcagttggg ctatctggaa caaaaatctt aaaatgccac ggcgtatgac
     9901 ttaattgttc accataccat atataaccat aaatcatccc taatatatta caaatgagta
     9961 gcatcattaa ccaagaacgt tgataaagtg tatattgcca aaatgtatta atcgtcatct
    10021 gctaagtcct caaattgatt atgtttattt actagcttga gtgtatttaa aatttgcgtt
    10081 agttgataaa aacgttgctt ttcattcatc tgtaaactta aatcaatatt gtgtaacaag
    10141 taatctatta ataacgcatg tttatgccga tctatagcca tactatttaa gtcatgaaga
    10201 taagtttgat aactgggcga ttccgttagt tgagcaacta ttaaatcatc tagcctttgc
    10261 tcacgctttg acacgtttgc gaagtgaatt tgaatatcaa aagcacagtt atgattagcg
    10321 atataatcaa atatttcatt tgtattcatt aactttatat tacgcttagt aaattgaatt
    10381 gcagaagcgt gacttcccac ttctgcaatt tctaatgttt catgatgatt aatttttgta
    10441 tctacaaaat gagtgtttgc caatttcgcc tcattcactt ttatatagtt aagcacccaa
    10501 actgcaatac gcgacttaaa tcgatattga aaaagtaaat attcaataaa actttcttta
    10561 atttgattga gtgtctctga catcaaatac cccattttaa gattgcaatc ttgataattc
    10621 gtcatgccaa ttttcgttac ttggctctag ttccaacaat tgatttaaaa tagtaattgc
    10681 ttgttccttt tgaccaattt caattaaata gaaataataa tcactcataa aatcaatatt
    10741 tgttttcatc gttggatatg ctaattcaaa gaaatgttga gcttctttat ctcgctcttc
    10801 ttgaccatag gcgaacgcta aatgccacat gaatgtagga tccaaatctc cttcatctac
    10861 atatgttaat aattcaatga ttgcttcata atcttcttca ttacgatata aatcgcttaa
    10921 aatcaataaa ggttcttggt aagcattatc aacctctaat gcttgtttta acaataatac
    10981 accttcatta gcatcgccgt gttctatttc caaacatcca gttgtataca ttaactcttt
    11041 ataaaattga cttagtcgta atccttcttt acccgtttca atagcatctg gataattttt
    11101 ttcattttca tataatgatt gtaaatacaa gtagccttga atataatcag gatctttaga
    11161 aagtaatgta gtcattattt taattgcttc ttgagtgatg tcatttttat cgtaagaaat
    11221 ggcttttttg agataatctt ctgaagtcat ttcatcttca ttaatttcat cgtataagcg
    11281 aatcgcatca ctatagttac cactttgtaa actacaatct gccatacgag agaataagtt
    11341 tacaccatta acttgatatt caccagtttc taaaacggtt tcgtattcag aggtagcacg
    11401 taaatattga ccatcataat ataacatttc agccaatgca aaatggatta ttggatcatt
    11461 tggctctagt tcaagtgctt cttgtaattt atcaatagca acttccatca tattaatttg
    11521 ttgatataaa tctgcttcta acatcaactt ttcaggtgat ggttcaacat aacttaaata
    11581 ttctaacgct tcgtcagttt gattttcaga cattaaacct tcaataaaat aaatcagcaa
    11641 ttcactttcg tctggatatt tgtgatataa cacgcggaat acttccaaac cttgtggcat
    11701 taatccaaaa ttgtaaagtg tctctcctag aataaataat gcgtcatcgt tgtcagtagt
    11761 tattgcttca ttaacacgag agtctaaatt ttctagtttt tgtagattga tatcgtctat
    11821 taatttatag atatcttcca ttttatatta tccctcattt tctaaaagct ttagttttgg
    11881 taaaaatcct ggaaatgata catttacggc atcaaattgt ttgattttga ctggctcgct
    11941 tgaaagtaga gaagcaactg caagcatcat tcctattcga tgatcagtta aactatcaac
    12001 tgttgcattt gttttaaatt ctgacggatg aataatcaat ccatcattag ttggttgtaa
    12061 ttcaaaccct aacaagttta acatatcagc cgttgtatca attctatttg tttcttttac
    12121 ttttaattcc tcggcatctt taattgtact cgtgccaact gcttgtgtac aaagtaatgc
    12181 tattacaggc agttcatcaa ttgcttttgg cactaattct ccttcgattg ttattggttg
    12241 aagcattggt gtgtattgta tacgaataga agcagtaggt tcagcaccag ttgtttgatt
    12301 gaaaagttga atattaccgc ccattttttc aacaatatca ataatacctg aacgtgttgg
    12361 attgattcca acattatgaa ttgttacatc acttcctggt gtgataagtg ctgcaacaat
    12421 aaagaacgct gcagatgaaa tatcgccagg aacatgaaaa tctgcaggtt taatgtatcg
    12481 aattgcttca ggggttgtag taattgataa cccttctgct tcaattggaa tattaaaatg
    12541 tctgaacatc gtctcagtat gatttcgact tacatctaat tctttaatga tggtcggttc
    12601 cttagaaaat agacttgcga ataagatggc acttttcact tgtgcacttg ccacttccat
    12661 cttgtaatta atacctttaa ttacagacgg tttaatgatt aatggggtgt aattgtcttc
    12721 aattccttca atatttgcat tcatactttt taacggtctt aaaacacgat ccatcggcct
    12781 tttacctatg gaaacatctc cagacaagac actttcaata cctaaaccac ttaacaaacc
    12841 tgccaataat cgtgttgtcg taccagaatt acctgtatac aatacttgat gtggcgtgtt
    12901 aaaagattga tatcctgggg aagtcacaac taatttttca tcatcttctt tgatttctac
    12961 acctaacagt cggaaaatgt ccatcgtacg acgacaatct tcgccaagta gtggcttata
    13021 tatagtagat acaccttcag ctagcgacgc caacatgatt gcacggtgtg tcattgattt
    13081 atcgcccggc acttctattt cgccctttaa cggacctgaa atatcaatga tttgttcatt
    13141 taccatttca ttcacctact taaaatatgt ttttaattgt tcacatgcat gttgtaatgt
    13201 tagttgatca acatgttgta caacgatatc tccaaaatgt ttaatcaaga ccatttgtac
    13261 accttgctta tcattctttt tatcacttag catgtattga tatatcgttt cgaaatctat
    13321 gtcggttatt gtttctaaag gataacctaa tttcgttaaa taattaatat aatgttggat
    13381 atcgtgatta gaattgaaca atatattcgc aacaataaat tgatatatta tgcctatcat
    13441 tacggcatga ccgtgtgcaa ttttgtggtt atactccacg gcatgaccaa atgtatgtcc
    13501 taaatttaaa tactttcgaa caccttgctc tttttcatca gcaacaacga tatttaattt
    13561 tgtttcaatg cctttagtta tatatttatc aattccattt aatgattgta atgacgctcg
    13621 atctttaaaa tgttgctcga tgtcatgtgt agcagattta ccatttaata aggcatgctt
    13681 atatacttcc gcatagccac tcaatatttc atcaaatggc aaagtattta aaaagtctaa
    13741 gtcataaatt acagcatctg gacgataaaa tgcaccgata aggtttttac cttgctttga
    13801 gttaataccc actttaccgc caacgctaga atcatgcgct agtatagtcg ttggcacttg
    13861 tataaagtga acacctcgta aaagtgtcgc cgcaataaat cctgcaaaat ctcctgtcgc
    13921 accaccacca acagctataa tcgctgtatt acgcgttaca tgatgtgaca aaatgtattc
    13981 taatgtttct tgatattgct caaatgtttt cgtcttttca ccagctggaa taataacttt
    14041 atgtacattt ccaaaagata aaatatcatt aaatttatca gcaaaatatt gatttacatg
    14101 ctcgtcaatt aatataaaac tttgatcaaa ctgatcaata tacgtgctaa tatggtcaat
    14161 tgcaccatgt tcaacaaata ttggataatt gtttgaagga taggttgttt gtaatttcat
    14221 gatcacacct caattgttct tgttgttaaa actcaatatt taattgtctg cgctcaataa
    14281 tttgttgttt aagttgctca atatgatttg attggaattc ttccaataat gctttcgcaa
    14341 tttcaaatgc tacgacatgt tcgcagacga tacttgctgc aggaacagca caactatcag
    14401 aacgttcaat tgttgcctta aagtcttctt tagtatttat atcaactgaa tttaatggtt
    14461 tatataacgt aggaataggt ttcattacac cattaacgat aattggcatt ccatttgaca
    14521 taccgccttc taaaccacct aagtgatttg acccacgata atagcccaat tcagtattgt
    14581 agagaatttc gtcttgaatt tcgctaccag gcttttcagc tgctttaaat ccttctccaa
    14641 aacttacacc tttaaatgca ttaatactaa cgacaccctg tgctattctt ccatctaatt
    14701 tacgatcata atgtacataa ctacctacac caacaggcat attttcaact acaacttgaa
    14761 ctacgccccc tattgaatca ccatcgtttt tcgcttcatc aattttatcg cgcattgctt
    14821 gtgcgatgcc atcatcaatt acacggacgt catttcgatc aaggtttgct ttaaatgttt
    14881 ctgaatcata aaaatcttta tctttaatgc cacctatctc aacaacacga ctgtatattt
    14941 cgatatctaa ttgttctaat aaaactttgc ataatgcacc gacagctaca cgtgctgctg
    15001 tttctctggc agatgaacgt tctaatacat ttcgtaagtc acgatgatta tatttcatac
    15061 cgccaactaa atctgcatgt cccggtcttg gcttcgtaat tgtacgtttc atattttctc
    15121 gttcttcgtc gcttattggc gcagcaccca taatttttcg ccaatgtgta aaatcatcat
    15181 tagtaacaac cattgtaata gggctaccta atgtataacc atttctcaca cccgaaacaa
    15241 tctctactgt atctttctca atttgcatgc gtcggccacg accataacca ccttgacgct
    15301 tgaacatttc tttattaata tcttcaactt taatttctaa atttgctggt ataccttcga
    15361 caataactgt taattgaggt ccatgtgatt ctcctgaagt tagatatctc atacacactc
    15421 gctcctttca tacttccgca ataacattat aatatgttgt taatcttatc acattcattg
    15481 tggattcata aatgtttgtt ttcaagctta attaagaaaa tgga
//
LOCUS       DAHHOJ010000065        15295 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.065, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000065 DAHHOJ010000000
VERSION     DAHHOJ010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.065"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            131..2227
                     /gene="pnp"
                     /locus_tag="NBH32_001692"
     CDS             131..2227
                     /gene="pnp"
                     /locus_tag="NBH32_001692"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCD1515285.1"
                     /translation="MSQEKKVFKTEWAGRSLTIETGQLAKQANGAVLVRYGDTVVLST
                     ATASKEPRDGDFFPLTVNYEEKMYAAGKIPGGFKKREGRPGDDATLTARLIDRPIRPL
                     FPKGYKHDVQIMNMVLSADPDCSPQMAAMIGSSMALSVSDIPFQGPIAGVNVGYIDGK
                     YIINPTVEEKEVSRLDLEVAGHKDAVNMVEAGASEITEQEMLEAIFFGHEEIQRLVEF
                     QQQIVDHIQPVKQEFIPAERDEALVERVKSLTEEKGLKETVLTFDKQQRDENLDNLKE
                     EIVNEFIDEEDPENELLIKEVYAILNELVKEEVRRLIADEKIRPDGRKPDEIRPLDSE
                     VGILPRTHGSGLFTRGQTQALSVLTLGALGDYQLIDGLGPEEEKRFMHHYNFPNFSVG
                     ETGPVRAPGRREIGHGALGERALKYIIPDTADFPYTIRIVSEVLESNGSSSQASICGS
                     TLALMDAGVPIKAPVAGIAMGLVTREDSYTILTDIQGMEDALGDMDFKVAGTKEGITA
                     IQMDIKIDGLTREIIEEALEQARRGRLEIMNHMLQTIDQPRTELSAYAPKVVTMTIKP
                     DKIRDVIGPGGKKINEIIDETGVKLDIEQDGTIFIGAVDQAMINRAREIIEEITREAE
                     VGQTYQATVKRIEKYGAFVGLFPGKDALLHISQISKNRIEKVEDVLKIGDTIEVKITE
                     IDKQGRVNASHRALEE"
     gene            2463..4136
                     /locus_tag="NBH32_001693"
     CDS             2463..4136
                     /locus_tag="NBH32_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002455407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HCD1515286.1"
                     /translation="MSLIKKKNKDIRIIPLGGVGEIAKNMYIVEVDDEMFMLDAGLMF
                     PEDEMLGIDIVIPDISYVLENKDKLKGIFLTHGHEHAIGAVSYVLEQLDAPVYGSKLT
                     IALIKENMKARNIDKKVRYYTVNNDSIMRFKNVNISFFNTTHSIPDSLGVCIHTSYGA
                     IVYTGEFKFDQSLHGHYAPDIKRMAEIGEEGVFVLISDSTEAEKPGYNTPENVIEHHM
                     YDAFAKVRGRLIVSCYASNFIRIQQVLNIASKLNRKVSFLGRSLESSFNIARKMGYFD
                     IPKDLLIPITEVDNYPKNEVIIIATGMQGEPVEALSQMAQHKHKIMNIEEGDSVFLAI
                     TASANMEVIIANTLNELVRAGAHIIPNNKKIHASSHGCMEELKMMINIMKPEYFIPVQ
                     GEFKMQIAHAKLAAEAGVAPEKIFLVEKGDVINYNGKDMILNEKVNSGNILIDGIGIG
                     DVGNIVLRDRHLLAEDGIFIAVVTLDPKNRRIAAGPEIQSRGFVYVRESEDLLREAEE
                     KVREIVEAGLQEKRIEWSEIKQNMRDQISKLLFESTKRRPMIIPVISEI"
     gene            4393..6762
                     /locus_tag="NBH32_001694"
     CDS             4393..6762
                     /locus_tag="NBH32_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1515287.1"
                     /translation="MAQAKKKSTAKKKTTSKKRTNSRKKKNDNPIRYVIAILVVVLMV
                     LGVFQLGIIGRLIDSFFNYLFGYSRYLTYILVLLATGFITYSKRIPKTRRTAGSIVLQ
                     IALLFVSQLVFHFNSGIKAEREPVLSYVYQSYQHSHFPNFGGGVLGFYLLELSVPLIS
                     LFGVCIITILLLCSSVILLTNHQHREVAKVVLENIKAWFGSFNEKMSEKNQEKQLKRE
                     EKARLKEEQKARQNEQPQIKDVSDFTEVPQERDIPIYGHTENESKSQSQPSRKKRVFD
                     AENSSNNIVNHHQADQQEQLTEQTHNSVESENTIEEAGEVTNVSYVVPPLTLLNQPAK
                     QKATSKAEVQRKGQVLENTLKDFGVNAKVTQIKIGPAVTQYEIQPAQGVKVSKIVNLH
                     NDIALALAAKDVRIEAPIPGRSAVGIEVPNEKISLVSLKEVLDEKFPSNNKLEVGLGR
                     DISGDPITVPLNEMPHLLVAGSTGSGKSVCINGIITSILLNAKPHEVKLMLIDPKMVE
                     LNVYNGIPHLLIPVVTNPHKAAQALEKIVAEMERRYDLFQHSSTRNIKGYNELIRKQN
                     QELDEKQPELPYIVVIVDELADLMMVAGKEVENAIQRVTQMARAAGIHLIVATQRPSV
                     DVITGIIKNNIPSRIAFAVSSQTDSRTIIGTGGAEKLLGKGDMLYVGNGDSSQTRIQG
                     AFLSDQEVQDVVNYVVEQQQANYVKEMEPDAPVDKSEMKSEDALYDEAYLFVVEQQKA
                     STSLLQRQFRIGYNRASRLMDDLERNQVIGPQKGSKPRQVLIDLNNDEV"
     gene            6767..7480
                     /locus_tag="NBH32_001695"
     CDS             6767..7480
                     /locus_tag="NBH32_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1515288.1"
                     /translation="MSEMNAVYNVKQYILNLIKQNKLEYGDQLPSNLSIARELNVKTD
                     DVYEAIQALITEQVIKDNFEEGTSVKSLPPFFYPLNELISIGQMIKNAGFECGTEYLN
                     FDEQPATMLDANLLSVEEGYPVTIIERLRTADGEPVVYCLDKIAKKELTCTEYQMSNG
                     SILSAIKEQSNHNICYADTEIEAVNYEPRISEVLNASPHEGLILLKITHYNESDEPIL
                     YSLNYMKNSLVQFKITRKI"
     gene            7511..8776
                     /locus_tag="NBH32_001696"
     CDS             7511..8776
                     /locus_tag="NBH32_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1515289.1"
                     /translation="MSRQSQPNIHIKVSPTTKFKTTTIVFKFMAPLEYDTITARSLLS
                     KLLVRATKKWPTDKSFNNHLADLYGAYVNSTISKFKDQHVITFSLEIVNERYLRNGES
                     LFNQGLDLLHEIIWNPLIENKAFNDNFVNQEKTLLAKKIEAMVDNKAQYSFLKLLDHM
                     FENEAYKYLSTGQLEQIPHITAETLYHTYQSMINNDQCSVYVVGNVEPESVEKQIREK
                     FALKPFDKHQFQHSTHHLHDEEVDYIVEYDDVDQAKLNMGYRFPTQYGQSGYAAFVVF
                     NMMFGGDPSSVLFNEVREKQSLAYSIHSQIDGKNGYLFVLSGVSSDKYETAKDTIISE
                     FEKIKAGDFTEEKLELAKKVIISHRYESEDRPKSIIEIMHNQILLEQPQSKETFINDI
                     QKVSREDIVTVAEKAFLDTIYVLTKGGDK"
     gene            8776..10062
                     /locus_tag="NBH32_001697"
     CDS             8776..10062
                     /locus_tag="NBH32_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCD1515290.1"
                     /translation="MKERYYELIDERVFEQELENGLRLFIIPKPGFQKTFVTYTTQFG
                     SLDNQFKPLGQDQFVTVPDGVAHFLEHKLFEKEEEDLFTAFAEDNAQANAFTSFDRTS
                     YLFSATDNIENNIKRLLTMVETPYFTKETVDKEKGIIAEEIKMYQEQPGYKLMFNTLR
                     AMYQQHPIRVDIAGSVESIYDITKDDLYLCYETFYHPSNMVLFVVGDVNPEEICRIVK
                     QHEDARNKVNQPKIERGLVDEPEDVKEAFVTESMKIQSPRLMLGFKNKPLQEAPQKYV
                     QRDLEMSLFFELIFGEETDFYQNLLNEGLIDDTFGYQFVLEPTYSFSIVTSATEEPDK
                     LKKLLLDELRDKKGNFQDAQAFELLKKQFIGEFISSLNSPEYIANQYTKLYFEGVSVF
                     DMLDIVENITLDSINETSSLYLNLDQQVDSRLEIKK"
     gene            10062..10766
                     /locus_tag="NBH32_001698"
     CDS             10062..10766
                     /locus_tag="NBH32_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1515291.1"
                     /translation="MKALVLGGSGSIGSEIVKQLLTDGFEVYVQYYRTDINELTSKFN
                     DDKVHFIQTDLSQTTDIDKTFGDIKSLDCLIYASGQSLYGVLQDMKDHDIDACYQLNV
                     LQLIRLCRYFVDVLRQSDNGRIIVISSIWGETGASMETIYSTMKSAQLGFVKALSQEL
                     ALTSVTVNAIAPGFVAGNMASEWQEDELQAMITELPQQRLVLPSEVAHTCAYLYHPNA
                     RSVTGTIQKVNGAWYI"
     gene            10871..11698
                     /locus_tag="NBH32_001699"
     CDS             10871..11698
                     /locus_tag="NBH32_001699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmfK family protein"
                     /protein_id="HCD1515292.1"
                     /translation="MTVAEKKEWYLEYEIEINRPGLLGDVSSLLGMLGISIVTINGVD
                     QGKRGLLIKTDNLEKVERFEQIARGINEIEITKLKKPELRDRLAVRHGRYIEQDAKDK
                     KTFRFEREDLGLLVDFLAELFKEEGHKLIGIRGMPRVGKTESIVAGSVCAHKRWLFIS
                     STLIKQTVRSSLIKGEYDANHVYIIDGAVTARESNPKHQELVNEVMTLPSIKVVEHPD
                     LFVETSTCTMEDFDYIIELRENENQEIHYEEMKKQTVQSKNNLDFGDPFGGGFGFFE"
     gene            11717..12109
                     /locus_tag="NBH32_001700"
     CDS             11717..12109
                     /locus_tag="NBH32_001700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1515293.1"
                     /translation="MKTVGEALKGRRERLGMTLTELEQRTGIKREMLVHIENNEFDQL
                     PNKNYSEGFIRKYASVVNIEPNQLIQAHQDEIPSNQAEWDEVITVFNNNKDLDYKSKS
                     KEPIQLLVIMGITVLITLLLWIMLVLIF"
     gene            12143..12721
                     /gene="pgsA"
                     /locus_tag="NBH32_001701"
     CDS             12143..12721
                     /gene="pgsA"
                     /locus_tag="NBH32_001701"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCD1515294.1"
                     /translation="MNIPNQITVFRVVLIPVFILFALVDFGFGNVSFLGGYEIRIELL
                     ISGFIFILASLSDFVDGYLARKWNLVTNMGKFLDPLADKLLVASALIVLVQLGLTNSV
                     VAIIIIAREFAVTGLRLLQIEQGFVSAAGQLGKIKTAVTMVAITWLLLGDPLATLIGL
                     SLGQILLYIGVIFTILSGIEYFYKGRDVFKQK"
     gene            12946..14097
                     /locus_tag="NBH32_001702"
     CDS             12946..14097
                     /locus_tag="NBH32_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HCD1515295.1"
                     /translation="MSIAIIAVGSELLLGQIANTNGQFLSKVFNEIGQNVLEHKVIGD
                     NKKRLESSVRHALEKYDTVILTGGLGPTKDDLTKHTVAQIVGKDLVIDEPSLKYIESY
                     FEEQGQEMTPNNKQQALVIEGSTVLANHHGMAPGMMVNFENKQIILLPGPPKEMQPMV
                     KNELLSHFINHNRIIHSELLRFAGIGESKVETILIDLIDKQTNPTIAPLAGSHEVYIR
                     LTANADSKEQAQSLIQPVKQEILDRIGEYYYGSDDTLIEQAVIKKIHEPFVIYDGITN
                     GALYHRLKEVDLNDVLKGMINHNENFVDINKPIEQQLKDAVQFVNKLFNVSSAIILLE
                     YDGVVHIGYDNNFEFKTEQFKMSKSRNLLKNRSQNYALIRLLNWLRTTN"
     gene            14262..>15295
                     /gene="recA"
                     /locus_tag="NBH32_001703"
     CDS             14262..>15295
                     /gene="recA"
                     /locus_tag="NBH32_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCD1515296.1"
                     /translation="MDNDRQKALDTVIKNMEKSFGKGAVMKLGDNIGRRVSTTSTGSV
                     TLDNALGVGGYPKGRIIEIYGPESSGKTTVALHAIAEVQSNGGVAAFIDAEHALDPEY
                     AQALGVDIDNLYLSQPDHGEQGLEIAEAFVRSGAVDIVVVDSVAALTPKAEIEGEMGD
                     THVGLQARLMSQALRKLSGAISKSNTTAIFINQIREKVGVMFGNPETTPGGRALKFYS
                     SVRLEVRRAEQLKQGQEIVGNRTKIKVVKNKVAPPFRVAEVDIMYGQGISKEGELIDL
                     GVENDIVDKSGAWYSYNGERMGQGKENVKMYLKENPQIKEEIDRKLREKLGISDGDVE
                     ETEDTPKSLF"
BASE COUNT         5573 a         2043 c         2960 g         4719 t
ORIGIN      
        1 aagtctattt tgtaaataat agatggatat cccgctctct ttttttccaa ttcaatattt
       61 tataactaat attaaaatac gataataaat gatatgatat aactattaga ttcaagagag
      121 gagatttata atgtctcaag aaaagaaagt ttttaaaact gaatgggcag gaagatcttt
      181 aacgattgaa acagggcaat tagctaaaca agcaaatggc gctgtattgg ttcgttatgg
      241 agatacagtc gtgttatcga cggcaactgc atcaaaagaa cctcgtgatg gagatttctt
      301 cccattaaca gtgaactatg aagaaaaaat gtacgctgcg ggtaaaattc ctggtggatt
      361 taaaaagaga gaaggacgtc ctggtgacga tgcaacatta actgcgcgat taattgatag
      421 accaattaga cctttattcc ctaaaggata taagcatgat gttcaaatta tgaacatggt
      481 attaagtgca gatcctgatt gttcaccaca aatggctgca atgattggtt catctatggc
      541 gcttagtgtg tcggatattc cattccaagg gccaatcgcc ggtgtaaatg tgggttatat
      601 tgacggtaaa tatatcatta acccaacagt agaagaaaaa gaagtttctc gtttagacct
      661 tgaagtagct ggtcataaag atgcagtaaa catggtagag gcaggcgcta gtgagattac
      721 tgaacaagaa atgttagagg cgattttctt tggtcatgaa gagattcaac gtttagttga
      781 gttccaacaa caaatcgtcg accacattca acctgttaaa caagaattta ttccagcaga
      841 gcgtgatgaa gcgctagttg aacgtgtaaa atctttaacc gaagaaaaag gacttaaaga
      901 aacagtttta acatttgata aacaacaacg agatgaaaat cttgataact taaaagaaga
      961 aatcgtcaat gaatttatcg atgaagaaga tccagagaat gaattactta ttaaagaagt
     1021 ttatgcaatt ttaaatgaat tagtgaaaga agaagttcga cgtttaattg cagatgaaaa
     1081 aattagacca gacggccgta aacctgatga aatccgtcca ttagattctg aagttggtat
     1141 tttacctaga acgcatggtt caggtctatt tacacgtggt cagactcaag cactttcagt
     1201 tttaacatta ggtgctttag gcgattatca attaattgat ggtttaggac ctgaagaaga
     1261 aaaaagattc atgcatcatt ataacttccc gaatttttca gtaggtgaaa ctggtccagt
     1321 acgtgcgcca ggtcgtcgtg aaattggaca tggtgcgtta ggtgaaagag cattaaaata
     1381 tattattcct gatactgctg atttcccata tacaattcgt attgtaagtg aggtacttga
     1441 atcaaacggt tcatcatctc aagcgtcaat ttgtggatca acattagcat taatggatgc
     1501 gggcgtaccg attaaagcac cagttgctgg tattgctatg ggccttgtta cacgtgaaga
     1561 tagctatacg attttaactg atatccaagg tatggaagat gcattaggtg atatggactt
     1621 taaagtcgct ggtactaaag aaggtattac agcaatccaa atggatatta aaattgatgg
     1681 tttaacgcgt gaaattatcg aagaggctct agaacaagcg agacgtggtc gtttagaaat
     1741 aatgaatcat atgttacaaa caattgatca accacgtact gaattaagtg cttacgcgcc
     1801 aaaagttgta actatgacaa ttaaaccaga taagattaga gatgttatcg gacctggtgg
     1861 taaaaaaatt aacgaaatta ttgatgaaac aggcgttaaa ttagatattg aacaagatgg
     1921 tactatcttt attggggccg ttgatcaagc tatgataaat cgtgctcgtg aaatcattga
     1981 ggaaattaca cgtgaagcgg aagtaggtca aacttatcaa gccactgtta aacgtattga
     2041 aaaatacggt gcgtttgtag gcctattccc gggtaaagat gcgttgcttc acatttcaca
     2101 aatttcaaaa aatagaattg aaaaagtgga agatgtatta aaaatcggtg acacaattga
     2161 agttaagatt actgaaattg ataaacaagg tcgagtaaat gcttcacaca gagcattaga
     2221 agaataatat ttaaagtcat atgacgacaa tgtatcgtca tgtgattttt ttatgcaatt
     2281 ttttacgaag tgatccattt tgaatttgtt gtattgaaca ttttaaaacg ctttattatt
     2341 ttgtgtgtaa ctgttaatta tcctgtatgt atagtgatta atagtgtaca tcaagtgttt
     2401 tttaacttat aatgaatagt gagtttatat atggacgggt aacaaattta ggaggtaaga
     2461 ttttgagttt aataaagaaa aagaataaag atattcgcat tataccatta ggcggtgttg
     2521 gcgaaattgc taaaaatatg tatatcgttg aagtagacga tgaaatgttt atgttagatg
     2581 ctggacttat gtttccagaa gacgaaatgc taggtattga tattgttata ccagacattt
     2641 catacgtact tgaaaataaa gataaattga agggtatatt ccttacacac ggacatgagc
     2701 acgcgattgg tgcagtgagt tatgttttag aacaattaga tgcaccagta tatggatcta
     2761 aattgacaat agcgttaatt aaagaaaata tgaaagcccg taatattgat aaaaaagttc
     2821 gctactacac agttaataat gattcaatta tgagattcaa aaacgtgaat attagtttct
     2881 ttaatacgac acacagtatt cctgatagtt taggtgtttg tattcatact tcatatggtg
     2941 ccattgtgta tacaggtgaa tttaagtttg accaaagttt acatggacat tatgcaccag
     3001 atattaaacg tatggcagag attggtgaag aaggcgtatt tgtcttaatc agtgattcta
     3061 ctgaggcaga gaaacctgga tataatactc cggaaaatgt gattgaacat catatgtatg
     3121 acgcttttgc aaaagtgcga ggtcgcttga ttgtttcatg ttatgcttcg aactttatac
     3181 gtatacagca agttttaaat attgctagca agctaaatcg taaagtgtca tttttaggaa
     3241 gatcacttga aagttcattt aatattgctc gtaaaatggg gtatttcgac attcctaaag
     3301 atttgctaat tcctataaca gaagttgata attatcctaa aaatgaagtg ataattatag
     3361 ctactggtat gcaaggagaa cctgtagaag ccttaagtca aatggcgcaa cataagcata
     3421 aaattatgaa tatcgaagaa ggcgattctg tatttttagc aattacggct tctgctaata
     3481 tggaagttat cattgcgaat acattaaatg agcttgtacg tgctggcgca catattattc
     3541 caaataacaa gaagattcat gcttcaagtc atggttgcat ggaagaatta aaaatgatga
     3601 ttaatattat gaaacctgaa tactttattc ctgtacaagg tgaatttaaa atgcagatag
     3661 cacatgcgaa gctagcagct gaagcaggtg ttgcaccaga aaagattttc cttgtggaaa
     3721 aaggagatgt cattaattac aacggtaaag atatgatatt aaatgaaaag gtaaattcag
     3781 gaaatatttt aatagatggt attggtattg gggatgtagg aaatatcgtg ttgagagacc
     3841 gtcatctttt agcagaagat ggtatcttta ttgctgtagt aacgttagat cctaaaaata
     3901 gacgtatagc tgcgggacct gaaattcaat ctcgtgggtt tgtatatgta cgtgaaagtg
     3961 aagacttatt acgtgaagca gaagagaaag tacgtgaaat agtagaggct ggtttacaag
     4021 aaaaacgcat agaatggtct gaaattaagc aaaatatgcg tgatcaaatt agtaaactat
     4081 tattcgaaag tacaaaacgt cgtcctatga ttattccagt aatttctgaa atttaatcaa
     4141 aaagtcatta acataaaaga ggtcagaaca agtcactgaa atataatggt tgtcatggac
     4201 aatttactta tattttatga tggtcaattg aaaggggtaa cgattaatct gttatcttaa
     4261 gtaaattgat acatagatga tattgttcta acctctttca tcgtctgttt ggactacata
     4321 ttctaaacat caaataggaa attatatata ataacgtcgt tttaactaag gcaacataag
     4381 gaggtgcgtc aattggcaca agcaaaaaag aaatcgacag ctaagaaaaa aacaacatca
     4441 aaaaaaagaa caaattcgag gaaaaagaag aatgataatc cgatacgtta tgtcatagct
     4501 attttagtag ttgtattaat ggtgttgggt gttttccaat taggaataat aggtcgtcta
     4561 attgacagct tctttaatta tttatttggg tacagtagat atttaacata tattttagta
     4621 ctcttagcaa ctggttttat tacatactct aaacgtattc ctaaaactag acgaacggct
     4681 ggttcgattg tattgcaaat tgcattgcta tttgtatcac agttagtttt tcattttaat
     4741 agtggtatca aagctgaaag agaacctgta ctttcttatg tgtatcagtc ataccaacac
     4801 agtcatttcc caaattttgg tggcggtgta ttaggctttt atttattaga gttaagcgta
     4861 cctttaattt cattatttgg tgtatgtatt attactattt tattattatg ctcaagtgtt
     4921 attttattaa caaaccatca acatcgtgaa gtagcaaaag ttgtactgga aaatataaaa
     4981 gcttggtttg gttcatttaa tgaaaaaatg tcggaaaaaa accaagaaaa acaattgaag
     5041 cgtgaagaaa aagcgagact taaagaagaa caaaaggcac gtcaaaatga acagccacaa
     5101 ataaaagatg tgagtgattt tacggaagtg cctcaagaaa gagatattcc aatttatggg
     5161 catactgaaa atgaaagtaa aagtcagagt caaccaagtc ggaaaaagcg agtgttcgat
     5221 gcagagaata gttcgaataa catcgtaaat catcatcaag cagatcagca agaacaatta
     5281 acagaacaaa ctcataacag tgttgaaagt gaaaacacta ttgaagaagc tggtgaagtt
     5341 acgaatgtat cgtatgttgt tccaccgtta actttactta atcaacctgc aaaacaaaaa
     5401 gcaacatcta aagctgaagt gcaacgtaaa ggacaagtac tagagaatac attaaaagat
     5461 tttggggtaa atgcaaaagt gacacaaatt aaaattggtc ctgcagtaac tcaatatgaa
     5521 attcaaccag ctcaaggggt taaagtgagt aaaattgtaa acctgcacaa tgatatcgca
     5581 ttagctttag cagcaaaaga tgttagaatc gaagcgccaa tacctggtcg ctctgcagta
     5641 ggtattgaag tgccaaatga gaaaatttca ttagtttcac taaaagaagt tttagatgaa
     5701 aaattcccgt ctaataataa actagaagtt ggattaggaa gagatatatc aggtgatcca
     5761 attactgttc cactaaatga aatgccacac ttattggtgg caggatcgac gggtagtggt
     5821 aaatctgttt gtataaatgg tattattaca agtattttat taaatgctaa accgcatgaa
     5881 gttaaactta tgttaatcga tccgaaaatg gttgaactaa atgtttataa cggaattcca
     5941 catttattaa ttccggttgt tacaaatcct cataaagctg ctcaagcttt agaaaaaatt
     6001 gtagctgaga tggaaagacg ttatgattta ttccaacatt catcaactag aaatattaaa
     6061 ggttataacg aattaatccg taagcaaaat caagaattag atgagaagca accagaatta
     6121 ccttatatcg ttgttattgt agatgagctt gcagatttaa tgatggtagc tggtaaagaa
     6181 gttgaaaatg cgattcaacg tgtcacacaa atggcacgtg cagcaggtat acatttgatt
     6241 gtagcgacac aaagaccttc tgtggatgta attacaggta tcattaaaaa taatattcca
     6301 tctagaatag cttttgctgt gagttctcaa acagattcaa gaactattat tggtactggc
     6361 ggcgcagaaa agttacttgg taaaggtgac atgttatacg ttggaaatgg tgactcatca
     6421 caaacacgta ttcaaggggc gtttttaagt gaccaagagg tgcaagatgt tgtaaattat
     6481 gtagtagaac aacaacaggc aaattatgta aaagaaatgg aaccagatgc accagtggat
     6541 aaatcggaaa tgaaaagtga agatgcttta tatgatgaag cgtatttgtt tgttgttgaa
     6601 caacaaaagg caagtacatc attgttacaa cgccaattta gaattggtta taatagagca
     6661 tctaggttga tggatgattt agaacgcaat caggtaatcg gtccacaaaa aggaagcaag
     6721 cctagacaag ttttaataga tcttaataat gacgaggtgt aaaaaaatgt cagaaatgaa
     6781 tgcggtatat aacgttaaac aatacatttt aaatttgatt aagcaaaata aattggaata
     6841 tggtgaccaa cttccaagta atttatcaat tgccagagaa ttaaatgtaa aaaccgacga
     6901 tgtttatgaa gcaattcagg cattgattac tgaacaagtc attaaagata attttgaaga
     6961 gggcacaagt gttaagtcac tgcccccttt cttttatcca ttgaatgaac ttataagtat
     7021 tgggcaaatg attaaaaatg caggatttga atgcggaact gaatacttaa attttgatga
     7081 gcaaccagca actatgttag atgcaaattt gttgagcgtt gaagaaggat atccagtaac
     7141 cattatagaa cgattacgaa ctgccgatgg agaaccggtc gtctattgtt tagataaaat
     7201 tgctaaaaaa gaattaacat gtacagagta tcaaatgagc aatggatcga ttctaagtgc
     7261 gataaaagaa caaagtaatc ataatatttg ttatgctgat acagaaattg aagcggtaaa
     7321 ttatgaacct cgaatatccg aagtactgaa tgcttcgcca cacgaaggtt tgattttatt
     7381 aaaaattacg cactataatg aatcagatga acctattttg tattcattaa attatatgaa
     7441 aaatagctta gttcaattta aaatcactag aaaaatatag atgtaagaag atatagagga
     7501 ggctatatat ttgagtagac aatctcaacc aaatatacat atcaaagttt caccaacaac
     7561 taaatttaaa acaactacta tagtttttaa atttatggca cctttagaat atgacacaat
     7621 aacagctaga tcattattaa gcaaattatt agttcgagca actaagaaat ggccaaccga
     7681 taagtcgttt aataatcact tagccgattt atatggtgcg tatgtgaata gtacaatttc
     7741 aaaattcaaa gatcagcatg tcattacatt ttcattagaa attgttaatg aacgttattt
     7801 aagaaacggt gaatcattat ttaatcaagg attagattta ttacacgaaa tcatttggaa
     7861 tccattaatt gaaaataaag cattcaatga taattttgtt aaccaagaga aaacattatt
     7921 agccaaaaaa atagaagcaa tggtagataa taaagcacaa tattcgtttt taaaattact
     7981 cgaccatatg tttgaaaatg aagcatataa atacttatct acaggacaac tagaacaaat
     8041 cccacatatt actgctgaaa cactatatca tacatatcaa tcaatgatta ataatgatca
     8101 atgttctgtt tatgttgtcg gcaatgtaga acctgaaagt gttgagaaac aaatacgtga
     8161 aaaatttgca cttaaaccat tcgataaaca tcaattccaa cattctactc atcatttaca
     8221 cgatgaagaa gttgattata ttgttgaata tgatgacgtg gatcaagcta aattaaatat
     8281 gggataccgt tttccaacac aatatggaca aagtggatat gctgcctttg ttgtatttaa
     8341 catgatgttt ggaggagatc cttcatctgt tttatttaat gaagtgcgag aaaagcaaag
     8401 tttagcgtac tctatacatt cacaaattga tggcaaaaat ggctatttat ttgttttgag
     8461 tggggtttca agtgataagt acgaaactgc aaaagatact attataagtg aatttgaaaa
     8521 aataaaagca ggagatttca ctgaagaaaa attagagtta gctaaaaaag taatcatttc
     8581 tcatcgatat gaatctgaag atcgtccgaa aagtattata gagattatgc ataaccaaat
     8641 attattagag caaccacaaa gcaaagaaac atttataaat gatatacaga aggtaagtcg
     8701 cgaagatatt gttactgttg ctgaaaaagc atttttagat acaatctatg tgttgacaaa
     8761 aggaggggat aaataatgaa agagcgttat tatgaattaa tagacgaaag agtattcgaa
     8821 caagaattag aaaatggttt acgattattt attatcccca aaccaggttt tcaaaagaca
     8881 tttgtcactt acactacaca atttggttca ttagataatc aattcaaacc cctcggacaa
     8941 gaccaatttg ttactgtacc tgatggagtt gctcactttt tagaacataa attatttgaa
     9001 aaagaagaag aagacttatt tactgcgttt gctgaagata atgcacaagc aaatgcgttt
     9061 acaagctttg atcgtacaag ctacttgttc agtgcaactg ataatattga aaacaacatt
     9121 aaacgtttac ttacaatggt tgaaacgcct tattttacaa aagaaactgt tgataaagaa
     9181 aaaggtatta ttgcagaaga aataaaaatg tatcaagaac aacctggata taaattaatg
     9241 tttaatacat tgcgtgcaat gtatcaacaa catccaatac gtgttgatat tgccggtagt
     9301 gtagaaagta tatacgatat tacaaaagat gatttgtatc tatgttatga aacgttttat
     9361 catccatcaa atatggtttt atttgttgtt ggtgatgtga atccagaaga aatatgtcga
     9421 atagtaaaac agcacgagga tgctcgtaat aaagtaaacc aacctaaaat cgaacgagga
     9481 cttgttgatg aaccggagga tgttaaagaa gcatttgtta ctgaatctat gaaaattcaa
     9541 tcaccaagac taatgcttgg ttttaaaaat aaaccattac aagaagcgcc tcaaaaatat
     9601 gtacaacgtg atttagaaat gtcattattc tttgagttaa tttttgggga agaaacagat
     9661 ttttatcaga atttattaaa cgaaggactt atcgatgata catttggtta tcaatttgta
     9721 ctagagccga cgtatagttt ttcaatcgtg acaagcgcta ctgaagaacc agataaatta
     9781 aaaaaattat tattagatga gttgcgtgat aaaaaaggca atttccaaga tgcacaagca
     9841 tttgaacttt tgaaaaagca atttataggt gaattcatat caagtttaaa ctcacctgaa
     9901 tatattgcta atcaatatac taaattgtat tttgaaggtg ttagcgtgtt tgacatgtta
     9961 gacattgttg aaaatatcac gttagatagt attaacgaaa cgtcatcgtt atatttaaat
    10021 ttagatcagc aagtcgatag tcgtttggag attaaaaagt aatgaaagca ttagtattag
    10081 gtggttctgg ttcaattggt tctgagatag tcaaacaatt attaactgat ggatttgaag
    10141 tttatgtgca atattatcgt actgatataa atgaattaac tagcaaattt aatgatgata
    10201 aagttcattt tatacaaacg gatttatctc aaacaactga tattgacaaa acatttggtg
    10261 acattaaatc attagactgt ttaatatatg caagtggtca gtctttatat ggtgttttac
    10321 aagatatgaa agaccatgat attgatgcat gttatcagtt aaatgtcttg caattaattc
    10381 gattatgtag atatttcgtt gatgttttac gtcaaagtga caatggaaga attattgtaa
    10441 tttcatcaat ttggggtgag acaggagcta gtatggaaac tatttattcg acgatgaaaa
    10501 gtgcacaatt aggtttcgtt aaggcgctta gtcaagagct tgcactaaca tcagtgacag
    10561 taaatgctat cgcacctgga tttgtagccg gtaatatggc aagtgagtgg caagaagatg
    10621 aacttcaagc aatgataact gaattaccac aacagcgatt ggttttaccg agtgaggttg
    10681 ctcatacatg cgcctattta tatcacccaa atgctagaag tgttactgga actatacaga
    10741 aagttaatgg tgcttggtat atataaaata tataactatt tttatgagca aatgttttta
    10801 tgattgatgt tataatatga atattacaat gaatcataat ttgttaatta aacaaggggt
    10861 gaggttcacc atgacagttg cagagaaaaa agaatggtac ctagaatacg aaattgaaat
    10921 taatagaccg ggtcttttag gtgatgtatc tagtttatta ggtatgttag gtataagtat
    10981 tgttacaatt aatggtgttg atcaaggtaa acgaggcctt ttaattaaaa ctgacaatct
    11041 tgaaaaagtt gaacgttttg agcaaatagc tcgtggtata aatgaaattg aaataacaaa
    11101 gcttaaaaaa ccagaattaa gagaccgtct tgcagttaga catggtagat atattgagca
    11161 agatgcaaaa gataagaaaa cctttcgatt tgagcgtgaa gatttaggtt tgttagtaga
    11221 ctttttagct gaattgttca aagaagaagg tcataagttg attggcatta gaggtatgcc
    11281 acgggttggt aaaactgaat caattgttgc gggaagtgtt tgtgcacata agagatggtt
    11341 atttattagt tctactttaa taaaacaaac tgtacgtagc tctctaatta aaggggaata
    11401 tgatgccaat catgtataca ttattgatgg tgcagttact gccagagaat ctaatccaaa
    11461 acatcaagag cttgttaacg aagttatgac gttaccatca atcaaagtcg ttgaacatcc
    11521 agatttattt gttgaaacaa gtacttgtac aatggaagat tttgactata ttatcgaatt
    11581 gagagaaaat gaaaatcaag aaatacatta cgaagaaatg aagaaacaga cagtccaaag
    11641 taagaataat ttagattttg gagatccatt tggtggtggt tttggtttct tcgagtaagt
    11701 gtaaggaggc tatgaattga aaacggtcgg tgaagcgcta aaaggtagac gtgaaaggtt
    11761 aggaatgact ttaacagaat tagagcaacg tactggaatt aaacgtgaaa tgctagtgca
    11821 tattgaaaat aatgaattcg atcaactacc gaataaaaat tacagcgaag gatttattag
    11881 aaaatatgca agcgtagtaa atattgaacc taaccaatta attcaagctc atcaagatga
    11941 aattccatcg aaccaagccg aatgggacga agtaattaca gttttcaata ataataaaga
    12001 tttagattat aagagtaaat caaaagagcc aatacaatta ttagtaatca tgggtattac
    12061 agttttaata actttattgt tatggatcat gttagtttta atattttaac agaaataaat
    12121 tagtgagaaa tgaggatgta taatgaatat tccgaaccag attacggttt ttagagtagt
    12181 gttaatacca gtttttatat tgtttgcgtt agttgatttt ggatttggca atgtgtcatt
    12241 tctaggagga tatgaaataa gaattgagtt attaatcagt ggttttattt ttatattggc
    12301 ttcccttagc gattttgttg atggttattt agctagaaaa tggaatttag ttacaaatat
    12361 ggggaaattt ttggatccat tagcggataa attattagtt gcaagtgctt taattgtact
    12421 tgtgcaacta ggactaacaa attctgtagt agcaatcatt attattgcca gagaatttgc
    12481 cgtaactggt ttacgtttac tacaaattga acaaggattc gtaagtgcag ctggtcaatt
    12541 aggtaaaatt aaaacagcag ttactatggt agcaattact tggttgttat taggtgatcc
    12601 attagcaaca ttgattggtt tgtcattagg acaaatttta ttatacattg gcgttatttt
    12661 tactatctta tctggtattg aatactttta taaaggtaga gatgttttta aacaaaaata
    12721 aatatttgtt tatactagat ttcattttca tatggaatct agttttttta atcccaattt
    12781 tagaaattag ccacgcaatt gtttataatg atatattgta aaacgatatt tgttcatttt
    12841 ttaggcaaat gtgttgtagc atctgataca ttgaatctaa aattgatgtg aatttttaaa
    12901 tgaaatacat gaaaaaatga attaaacgat acaaggggga tataaatgtc aattgccatt
    12961 attgctgtag gctcagaact attgctaggt caaatcgcta ataccaacgg acaatttcta
    13021 tctaaagtat ttaatgaaat tggacaaaat gtattagaac ataaagttat tggagataat
    13081 aaaaaacgtt tagaatcaag tgtacgtcat gcgttagaaa aatatgatac tgttatttta
    13141 acaggtggct taggtcctac gaaagatgac ttaacgaagc atacagtggc ccagattgtt
    13201 ggtaaagatt tagttattga tgagccttct ttaaaatata ttgaaagcta ttttgaggaa
    13261 caaggacaag aaatgacacc taataataaa caacaggctt tagtaattga aggttcaact
    13321 gtattagcaa atcatcatgg catggctcca ggaatgatgg tgaattttga aaacaaacaa
    13381 attattttat taccaggtcc accgaaagaa atgcaaccaa tggtgaaaaa tgaattgttg
    13441 tcacatttta taaaccataa tcgaattata cattctgaac tattaagatt tgcgggaata
    13501 ggtgaatcta aagtagaaac aatattaata gatcttatcg ataaacagac taatcctacg
    13561 attgcgcctt tggcgggaag tcatgaagta tatattagat tgactgcaaa tgccgactca
    13621 aaagaacaag cacaatcatt gattcaacct gttaaacaag aaattcttga tcgtattgga
    13681 gaatattatt atggttcaga tgacacatta attgagcaag ctgtaataaa gaaaattcat
    13741 gaaccttttg taatatatga tggtattact aatggtgctt tatatcatcg attgaaagaa
    13801 gtggatttaa acgatgttct aaagggtatg attaatcaca atgaaaactt tgttgatatt
    13861 aataaaccta ttgagcagca attaaaagat gcagtgcaat ttgttaataa attgtttaat
    13921 gtgtcatcag caattattct attagagtat gatggtgtag tccatatagg ctatgataat
    13981 aactttgaat ttaaaactga gcaatttaaa atgtctaaat ctagaaattt attaaagaac
    14041 agaagtcaaa attatgcgct cataagatta ttaaattggc ttagaacaac aaattaattg
    14101 tattatcgat aaaaatataa gcacgtttgt tcgtttttgc gttttgattt caagatttta
    14161 tacgaacaaa tattcgcaaa acacttgtat tttattttga atccttgtat agtattggta
    14221 agataattaa aagatagcaa tttcaattag gaggtctcgc tttggataac gatcgtcaaa
    14281 aagctttaga tacagtaatt aaaaatatgg agaaatcttt cggtaaaggt gccgtaatga
    14341 agttgggtga caatataggt cgccgagttt caactacatc aactggttca gttacattag
    14401 ataatgcgct aggtgtaggt ggctatccta aaggacgaat tattgaaatt tatggtcctg
    14461 aaagttctgg taagacaaca gtagcgcttc acgctattgc tgaagtacaa agtaatggcg
    14521 gggtggcagc atttatcgat gctgaacatg ctttagatcc agaatatgct caagcattag
    14581 gcgtggatat cgataattta tatttatcgc aaccggatca tggtgaacaa ggtcttgaaa
    14641 tcgccgaagc atttgttaga agtggtgcag ttgatattgt agttgtagac tcagttgctg
    14701 ctttaacacc taaagctgaa attgaaggag aaatgggaga cactcacgtt ggtttacaag
    14761 ctcgtttaat gtcacaagcg ttacgtaaac tttcaggtgc tatttctaaa tcaaatacaa
    14821 ctgctatttt catcaaccaa attcgtgaaa aagttggtgt tatgttcggt aatccagaga
    14881 ctacaccagg tggacgtgca ttaaaattct atagttcagt aagactagaa gtacgtcgtg
    14941 cagaacagct taaacaagga caagaaattg taggtaatag aactaaaatt aaagtcgtta
    15001 aaaataaagt ggcaccacca tttagagtag ctgaagttga tattatgtat ggacaaggta
    15061 tttctaaaga gggtgaactt attgatttag gtgttgaaaa cgacatcgtt gataaatcag
    15121 gagcatggta ttcttacaat ggcgaacgaa tgggtcaagg taaggaaaat gttaaaatgt
    15181 acttgaaaga aaatccacaa attaaagaag aaattgatcg taaattgaga gaaaaattag
    15241 gtatatctga tggtgatgtt gaagaaacag aagatacacc aaagtcatta tttga
//
LOCUS       DAHHOJ010000066        15218 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.066, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000066 DAHHOJ010000000
VERSION     DAHHOJ010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15218
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.066"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            109..687
                     /locus_tag="NBH32_001704"
     CDS             109..687
                     /locus_tag="NBH32_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCD1515297.1"
                     /translation="MTKRPKRILATIIIFLSLLFTIIYIDDIQKWFNQYTDKLTQNHK
                     GQGHSKWEDFFRGSRITETFGKYQHSPFDGKHYGIDFALPKGTPIKAPTNGKVTRFFN
                     NELGGKVLQIAEDNGEYHQWYLHLDKYNVKVGDRVKAGDIIAYSGNTGKQTTGAHLHF
                     QRMKGGVGNAYAEDPKPFIDQLPDGERSLYDL"
     gene            1070..2167
                     /gene="ugpC"
                     /locus_tag="NBH32_001705"
     CDS             1070..2167
                     /gene="ugpC"
                     /locus_tag="NBH32_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HCD1515298.1"
                     /translation="MAELKLEHIKKTYDNNNTVVKDFNLHITDKEFIVFVGPSGCGKS
                     TTLRMVAGLESITSGDFYIDGERMNDVEPKNRDIAMVFQNYALYPHMTVFENMAFGLK
                     LRKVNKKEIEQKVNEAAEILGLTEYLGRKPKALSGGQRQRVALGRAIVRDAKVFLMDE
                     PLSNLDAKLRVQMRTEILKLHKRLNTTTIYVTHDQTEALTMASRIVVLKDGDIMQVGT
                     PREIYDAPNCIFVAQFIGSPAMNMLNATVEMDGLKVGTHHFKLHNKKFEKLKAAGYLD
                     KEIILGIRAEDIHEEPIFIQTSPETQFESEVVVSELLGSEIMVHSTFQGMELISKLDS
                     RTQVMANDKITLAFDMNKCHFFDEKTGNRIV"
     gene            2180..3451
                     /locus_tag="NBH32_001706"
     CDS             2180..3451
                     /locus_tag="NBH32_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1515299.1"
                     /translation="MSKILKCITLAVVMLLIVTACGPNRSKEDIDKALNKDNSKDKPN
                     QLTMWVDGDKQMAFYKKITDQYTKKTGIKVKLVNIGQNDQLENISLDAPVGKGPDIFF
                     LAHDNTGSAYLQGLAAEIKLSKDELKGFNKQALKAMNYDNKQLALPAIVETTALFYNK
                     KLVKNAPQTLEEVEANAAKLTDSKKKQYGMLFDAKNFYFNYPFLFGNDDYIFKKNGSE
                     YDIHQLGLNSKHVVKNAERLQKWYDKGYLPKAATHDVMIGLFKEGKVGQFVTGPWNIN
                     EYQETFGKDLGVTTLPTDGGKPMKPFLGVRGWYLSEYSKHKYWAKDLMLYITSKDTLQ
                     KYTDEMSEITGRVDVKSSNPNLKVFEKQARHAEPMPNIPEMRQVWEPMGNASIFISNG
                     KNPKQALDEATNDITQNIKILHPSQNDKKGD"
     gene            3454..4722
                     /locus_tag="NBH32_001707"
     CDS             3454..4722
                     /locus_tag="NBH32_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1515300.1"
                     /translation="MTKRNPKLAALLSVIPGLGQFYNKRPIKGTIFFIFFISFISVFY
                     SFLNIGFWGLFTLGTVPKLDDSRVLLAQGIISILLVAFAIMLYVINILDAYRNAERFN
                     RNEEIKDPKARMVATWDKTFPYLLISPGTFLLIFVVVFPLIFMFGVAFTNYNLYNAPP
                     RHTLEWVGLDNFKTLFTIGVWRKTFFSVITWTLVWTLVATTLQIALGLFLAIIVNHPV
                     VKGKKFIRTVLILPWAVPSFVTILIFVALFNDEFGAINNDILQPLLGVAPAWLSDPFW
                     AKVALIGIQVWLGFPFVFALFTGVLQSISSDWYEAADMDGASSWQKFRNITFPHVIYA
                     TAPLLIMQYAGNFNNFNLIYLFNKGGPPVSGQNAGSTDILISWVYNLTFEFNNFNMGA
                     VVSLIIGFIVAIVAFIQFRRTSTFKDEGGL"
     gene            4724..5563
                     /locus_tag="NBH32_001708"
     CDS             4724..5563
                     /locus_tag="NBH32_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCD1515301.1"
                     /translation="MTKKKNILKAIGIYSFIAMMFVIILYPLLWTFGISLNPGTNLYG
                     AKMIPDNATFKNYAFLLFDDSSQYLTWYKNTLIVASANALFSVIFVTLTAYAFSRYRF
                     VGRKYGLITFLILQMFPVLMAMVAIYILLNTIGLLDSLFGLTLVYIGGSIPMNAFLVK
                     GYFDTIPKELDESAKIDGAGHMRIFLQIMLPLAKPILAVVALFNFMGPFMDFILPKIL
                     LRSPEKFTLAVGLFNFINDKYANNFTVFAAGAIMIAVPIAIVFLFLQRYLVSGLTTGA
                     TKG"
     gene            5637..6713
                     /locus_tag="NBH32_001709"
     CDS             5637..6713
                     /locus_tag="NBH32_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1515302.1"
                     /translation="MTIKVGIIGCGGIANGKHMPSLQKVENVEMIAFCDVDISKAASA
                     AEAYGTDNAKVYDDYKALLKDDTIDVIHVCTPNDSHCEITVAGLHAGKHVMCEKPMAK
                     TTAEAQKMIDTAKSTGKKLTIGYQNRFRPDSQFLHQAAQRGDLGDIYFGKAHAIRRRA
                     VPTWGVFLDEEAQGGGPLIDIGTHALDLTLWMMDNYEPESVMGSTFHKLNKQHHAANA
                     WGSWNPDEFTVEDSAFGFIKMKNGATIILESAWAINSLEVDEAKCSLSGTKAGADMKD
                     GLRIHGEDMGTLYTKHVELESKGVDFYEGNEVDEAEEEAKAWIDAVVNDTEPVVKPEQ
                     AMVVTKILEAIYQSAKSGKAIYFE"
     gene            6738..7778
                     /locus_tag="NBH32_001710"
     CDS             6738..7778
                     /locus_tag="NBH32_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCD1515303.1"
                     /translation="MTKLKVGVIGVGGIAQDRHIPALLKLKDTVSLVAVQDINTVQMI
                     DVAKRFNIPQAVETPSELFKLVDAVVICTPNKFHADLSIEALNHGVHVLCEKPMAMTT
                     EECDRMIEAANKNHKLLTVAYHYRHTDVAMTAKKAIEAGVVGKPLVARVQAMRRRKVP
                     GWGVFTNKALQGGGSLIDYGCHLLDLSLWLLGKDMVPHEVLGKTYNQLSKQPNQINDW
                     GTFDHTKFDVDDHVTSYMTFANRASMQFECSWSANIKEDKVHVSLSGEDGGINLFPFE
                     IYEPRFGTIFESKANVEHNEDIAGERQARNFVNACLGIEEIVVKPEEARNVNALIEAI
                     YRSDLDNKSIQL"
     gene            7833..8801
                     /locus_tag="NBH32_001711"
     CDS             7833..8801
                     /locus_tag="NBH32_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCD1515304.1"
                     /translation="MKIGVFSVLFYDKNFEDMLDYVAESGLDMIEVGTGGNPGDKFCK
                     LDELLENEDKRQAFMKSITDRGLQISGFSCHNNPISPDPIEAKEADETLRKTIRLANL
                     LDVPVVNTFSGIAGSDDTAKKPNWPVTPWPTAYSEIYDYQWNEKLIPYWQDLAEFAKE
                     QDVKIAIELHAGFLVHTPYTMLKLREATNEYIGANLDPSHLWWQGIDPIAAIRILGQA
                     NAIHHFHAKDTYINQENVNMYGLTDMQPYGNVATRAWTFRTVGYGHSPYVWADIISQL
                     IINGYDYVLSIEHEDPIMSVEEGFQKACQTLKSVNIYDKPADMWWA"
     gene            complement(9162..9656)
                     /locus_tag="NBH32_001712"
     CDS             complement(9162..9656)
                     /locus_tag="NBH32_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002454321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxyl methyltransferase
                     family protein"
                     /protein_id="HCD1515305.1"
                     /translation="MILSILLIFFCIRLVSLKISINHSKQLKADGAVEYGVKNSKFLA
                     ITHVLIYVLAGVEAFINKDTFSFANGIGLVILIFAYIMLFMVIKTLGGIWTLKLFILP
                     NHPIIKSGLYKITKHPNYFLNIIPELIGVLLLTHATYTTILLVPYAYFLYVRIKQEEK
                     LMNI"
     gene            9889..11268
                     /gene="uhpT"
                     /locus_tag="NBH32_001713"
     CDS             9889..11268
                     /gene="uhpT"
                     /locus_tag="NBH32_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCD1515306.1"
                     /translation="MNFFDIHKIPNKGIPLSVQRKLWLRNFMQAFFVVFFVYMAMYLI
                     RNNFKAAQPFLKEEIGLSTLELGYIGLAFSITYGLGKTLLGYFVDGRNTKRIISFLLI
                     LSAITVLIMGFVLSYFGSVMGLLIVLWGLNGVFQSVGGPASYSTISRWAPRTKRGRYL
                     GFWNTSHNIGGAIAGGVALWGANVFFHGNVIGMFIFPSVIALLIGIATLFIGKDDPEE
                     LGWNRAEEIWEEPVDKENIDSQGMTKWEIFKKYILGNPVIWILCVSNVFVYIVRIGID
                     NWAPLYVSEHLHFSKGDAVNTIFYFEIGALVASLLWGYVSDLLKGRRAIVAIGCMFMI
                     TFVVLFYTNATSVMMVNISLFALGALIFGPQLLIGVSLTGFVPKNAISVANGMTGSFA
                     YLFGDSMAKVGLAAIADPTRNGLNIFGYTLSGWTDVFIVFYVALFLGMILLGIVAFYE
                     EKKIRSLKI"
     gene            complement(11627..12385)
                     /locus_tag="NBH32_001714"
     CDS             complement(11627..12385)
                     /locus_tag="NBH32_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1515307.1"
                     /translation="MFKVVICDDERIIREGLKQIIPWGDYHFNTIYTAKDGVEALSLI
                     QQHQPELVITDIRMPRKNGVDLLNDIAHLDCNVIILSSYDDFEYMKAGIQHHVLDYLL
                     KPVDHAQLEVILGRLVRTLLEQQSQNGRSLASCHDAFQPLLKVEYDDYYVNQIVDQIK
                     QSYQTKVTVSDLIQHIDVSESYAMRTFKDHVGITIVDYLNRYRILQSLQLLDRHYKHY
                     EIADKVGFSEYKMFSYHFKKYLQMSPSDYCKQAK"
     gene            complement(12378..13934)
                     /locus_tag="NBH32_001715"
     CDS             complement(12378..13934)
                     /locus_tag="NBH32_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1515308.1"
                     /translation="MTAYKPYRHQLRRSLFASTIFPVFLVLIIGLVSFYAIYIWIEHR
                     TIHQHVDESQSSLHHTEKQIQTFITQHNNSFQELDLTNHHDVTATKRELLKLIHQQPA
                     TLYYELSGPNQFITNNYEHLNTKNMYLFSTHQLKFKNSTYMLKIYMANTPRLIEIKKD
                     SRQFALIVDQYDNILYANDDRFTIGEKYRPQQFGFMNESVKLNHADHRLIIYKDIHEN
                     IEDGITLLIVMAVVLVLLVIFGFISADNMAKRQTKDIETIIQKIYYAKNRHLGTYTPL
                     KNNSELEEINNYIYDLFESNEQLIHSIEHTERRLRDIQLKEIERQFQPHFLFNTMQTI
                     QYLITLSPKLAQTVVQQLSQMLRYSLRTNSHTVELNEELNYIEQYVAIQNIRFDDMIK
                     LHIESSEEARHQTIGKMMLQPLIENAIKHGRDTESLDITIRLTLARQNLHVLVCDNGI
                     GMSSSRLQYVRQSLNNDVFDTKHLGLNHLHNKAMIQYGSHARLHIFSKRNQGTLICYK
                     IPLSRGNVDV"
     gene            complement(13931..14899)
                     /locus_tag="NBH32_001716"
     CDS             complement(13931..14899)
                     /locus_tag="NBH32_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HCD1515309.1"
                     /translation="MKSKIYILLLFLIFLSACANTRHSESDKNVLTVYSPYQSNLIRP
                     ILNDFEKQEHVKIEIKHGSTQVLLSNLHNEDLSERGDVFMGGVLSETIDHPEDFVPYQ
                     DTSVTQQLEDYRSNNKYVTSFLLMPTVIVVNSDLQGDITIRGYQDLLKPILKGKIAYS
                     NPNTTTTGYQHMRAIYSMHHRVSDVHQFQDHAMQLSKTSKVIEDVAKGKYYAGLSYEQ
                     DARTWKNKGYPVSIVYPIEGTMLNVDGIALVKNAHPHPKRKKLVQYLTSRSVQQRLVA
                     EFDAKSIRKDVSEQSDQSIENLKNIPLIPKSKLPDIPHHKFLEMIQ"
BASE COUNT         5032 a         2309 c         2951 g         4926 t
ORIGIN      
        1 ttcatctata tttggcagta ctactaaagt atgagtgcat ttaattatga aatagttgat
       61 ttagaatata tacttaatac ccaaaatata tgaaggatgg atgccactat gacaaagcga
      121 ccaaaacgta ttttggcaac aattatcatt tttctttcac tattatttac gattatttat
      181 atagatgaca ttcaaaaatg gtttaaccaa tataccgata aattgacaca aaatcataaa
      241 ggacaaggac actcaaaatg ggaagacttt tttagaggga gtcggattac tgagactttt
      301 ggtaaatatc aacattcacc atttgatggt aagcattatg gcattgattt tgcattgcca
      361 aaaggtacac caattaaagc gccgacgaat ggtaaagtaa cacgtttctt taataatgaa
      421 ttaggcggca aggtattaca gattgccgaa gacaatggag aatatcacca gtggtatcta
      481 cacttagaca aatataatgt caaagtaggt gatcgagtca aagcaggtga tattattgca
      541 tattcaggca atacaggtaa acaaacgaca ggcgcacatt tacattttca aagaatgaag
      601 ggtggcgtag gtaatgcata tgcagaagat cctaaaccgt ttattgatca gttacctgat
      661 ggggaacgta gcctatatga tttgtagcta tagaagagtg cccgcagtct aaaaaattaa
      721 gcaatcattg tgtgagtatg atacttacac aatgattgct tttttcaatg aaaatcgaaa
      781 tgctaaggca taattgtttg atttagatat tacttaaaat gtaagacaag gttgttagca
      841 ttggcagtga aatatcgcac gtaaaaaatc attattgcca caccaaaaat agttgtgcgc
      901 tatatcaatt ttctgtataa aagtttaatt ctgacagtaa tgtaaacgtt tacaatttat
      961 gattgacatt aataataagt gactatatga tttatgtaag tatttatgca aacgttttca
     1021 caaagtgtat tgcacaatca aactgtaaac aaagtatggg aggcataaca tggcagaact
     1081 aaagttagag catattaaaa agacgtatga taacaacaat actgtagtga aagatttcaa
     1141 tctacatatt actgacaaag aattcattgt atttgttgga ccatcgggat gtggtaaatc
     1201 aacaacatta cgaatggttg ctggactaga gtctatcaca tctggagatt tttatattga
     1261 tggggaacgc atgaacgatg ttgaaccaaa gaatagagat attgcgatgg tatttcaaaa
     1321 ctatgcatta tatccacata tgactgtttt tgaaaatatg gcatttgggc taaagctacg
     1381 taaagtaaat aaaaaagaga ttgaacaaaa agttaatgaa gcagctgaaa tattaggatt
     1441 aactgagtat cttggtcgta aaccaaaagc gttatctggt ggacagcgtc aacgtgttgc
     1501 tttaggtaga gctattgtta gggatgcgaa agtcttttta atggatgaac cattatcgaa
     1561 tcttgatgcg aagcttcgag tacaaatgcg cacagaaata ttgaaattac ataagcgact
     1621 taatactacg acaatttatg ttacacatga tcaaactgaa gcattgacga tggctagtcg
     1681 aattgttgtt ttgaaagatg gcgacattat gcaagtcggc acacctagag aaatatatga
     1741 tgcccctaat tgcatatttg tggcgcaatt tatcggctca ccagcaatga atatgttgaa
     1801 tgctacagtt gaaatggacg gattgaaggt aggaacacac cattttaaat tacataataa
     1861 aaaatttgaa aagttaaaag ctgctggcta cttagataag gaaattattt taggtattcg
     1921 agctgaagac attcatgaag aaccaatatt tattcaaacc tctccagaga cacaatttga
     1981 atctgaagta gttgtatccg aactgttagg ttcagaaatt atggtacata gcacattcca
     2041 aggaatggaa ttgatttcta aattagattc aagaacacaa gtgatggcga acgacaagat
     2101 tacactagca tttgatatga ataagtgtca cttttttgat gaaaaaacag gaaatcgtat
     2161 cgtctaaggg ggagtattca tgtctaaaat tttaaaatgt atcacgttag ccgtggtaat
     2221 gttattaatc gtaactgcat gtggccctaa tcgttcgaaa gaagatattg ataaagcatt
     2281 gaataaagat aattctaaag acaagcctaa ccaacttacg atgtgggtgg atggcgacaa
     2341 gcaaatggcg ttttataaaa aaattacgga tcaatatact aaaaaaactg gcatcaaagt
     2401 aaagcttgta aatattggtc aaaatgatca actagaaaat atttcgctag acgctcctgt
     2461 aggaaaaggt ccagatatct ttttcttagc acatgataat actggaagtg cctatctaca
     2521 aggcttagct gctgaaatca aattatcaaa agatgagttg aaaggtttca ataagcaagc
     2581 acttaaagcg atgaattatg acaataagca actagcattg ccagctatcg tagaaacaac
     2641 cgcacttttc tataataaaa aattagtgaa aaatgcaccg caaacgttag aagaagttga
     2701 agctaatgct gccaaactaa ctgatagtaa aaagaaacaa tacggtatgt tatttgatgc
     2761 taaaaatttc tattttaatt atccgttttt attcggcaat gatgattata ttttcaagaa
     2821 aaatggcagt gaatatgata ttcatcagct aggactaaat tcaaaacatg tcgtcaaaaa
     2881 tgctgaacga ttacaaaaat ggtacgacaa agggtatctt cctaaggcag caacacatga
     2941 tgtcatgatt ggtcttttta aagaaggaaa agtaggacaa tttgtcactg gaccgtggaa
     3001 cattaatgaa tatcaagaaa cgtttggtaa agatttagga gtaacaacat tacctacaga
     3061 tggtggcaaa cctatgaaac catttctcgg tgtacgtggt tggtatttat ctgaatatag
     3121 taaacataag tattgggcta aagatttaat gttgtatatc actagtaaag atacattaca
     3181 aaaatataca gatgaaatga gcgaaattac tggacgtgtt gacgtgaaat catctaatcc
     3241 aaatttaaaa gtgtttgaaa agcaagcacg tcatgctgaa ccgatgccta atattcctga
     3301 aatgcgacaa gtttgggaac cgatgggcaa tgcaagcata tttatctcaa atggtaagaa
     3361 tcctaaacaa gcgttagatg aggcgacgaa tgatataacg caaaatatta agattcttca
     3421 tccatcacaa aatgataaga aaggagatta gttatgacga aacgtaaccc taaattagca
     3481 gcattattat ctgttatacc tggtttggga cagttttata ataaaagacc cattaaaggg
     3541 acgatatttt ttatcttttt catcagtttt atttctgtat tttatagctt tttaaatatt
     3601 ggtttttggg gattgttcac attagggaca gtacctaagt tagacgattc tcgtgtctta
     3661 cttgcacaag gtattatttc tatcttactc gttgcttttg caatcatgct atatgtcatt
     3721 aatattttag atgcatatcg taatgctgaa cgatttaatc gcaatgagga aataaaagat
     3781 ccgaaggcac gtatggttgc aacatgggat aagactttcc catacttact aatctcacca
     3841 ggtacattct tattgatatt tgtagttgta tttccattaa tatttatgtt tggagtagca
     3901 tttacaaatt acaatttata caacgcgcct ccgagacaca cattagaatg ggtcggttta
     3961 gataacttca aaacgttatt cacaattggc gtttggcgta aaacattttt cagtgttatt
     4021 acttggacat tagtatggac gcttgttgca acgacacttc aaattgcatt agggctgttt
     4081 ttggcaatta ttgtaaatca ccctgttgtc aaaggtaaga aatttatccg tactgtgtta
     4141 atcctacctt gggctgtacc atcatttgtg acaattttaa tatttgtagc gttatttaat
     4201 gatgaatttg gtgcgataaa taatgatatt ttgcaacctt tattaggtgt agcaccagca
     4261 tggttaagtg atccgttttg ggcaaaagtg gcattaatcg gcattcaagt atggcttggt
     4321 ttcccatttg tctttgcact gttcactggt gtactgcaaa gtatttcatc agattggtac
     4381 gaagcagcag atatggatgg tgcgtctagt tggcaaaagt ttagaaatat cacatttcca
     4441 catgtcattt acgccacagc gccattgtta attatgcaat atgcaggtaa tttcaataat
     4501 tttaatctta tttatctatt taataaaggt ggtccaccag tgtcagggca gaatgctgga
     4561 agtacagata tcttgatatc ttgggtgtat aatctgacat ttgagtttaa caactttaac
     4621 atgggtgcag ttgtgtcatt aattattgga tttattgttg ctattgtcgc atttattcaa
     4681 ttcagacgta caagtacgtt taaagatgag ggaggtttat aagatgacaa agaagaaaaa
     4741 catattaaaa gcaatcggta tttacagttt tatagcgatg atgtttgtca tcattttata
     4801 tccactactg tggacatttg gcatttccct taatccaggt acgaacttgt atggtgccaa
     4861 aatgatacca gacaatgcaa catttaaaaa ttatgcattc ttactattcg atgacagtag
     4921 tcaatacctg acttggtata aaaatacgct tatcgtagca tctgcaaatg cactgtttag
     4981 tgtgatattt gtcacgttaa cagcatatgc tttttctaga tatcgctttg ttggtcgtaa
     5041 atacgggctg attacatttt tgattttaca aatgttccct gtattaatgg caatggtcgc
     5101 aatctatatt ttgctaaata caattggatt attagattct ttatttggac taacactggt
     5161 atatattggt ggatcaatac cgatgaatgc ctttttagtg aaaggttact tcgatacgat
     5221 tccaaaagaa cttgatgaat ctgccaaaat tgatggtgca gggcatatgc gtattttctt
     5281 acaaattatg cttccattag ctaagccgat tttagcagtt gttgctttgt tcaattttat
     5341 ggggccattt atggacttta tattacctaa aatactatta agaagtcctg aaaaattcac
     5401 attagcagtt ggattgttca actttattaa tgataagtat gcaaataatt tcacagtgtt
     5461 tgcagcaggg gcaattatga ttgcagtacc tatagcaatc gtattcttgt tcttgcaacg
     5521 ctatttagta tcaggtttaa caacaggtgc gacaaaaggt tagtttgaaa ttaggagtgg
     5581 ggcagaattg ataaagaacc actaatgacg ataaagatta aaaggaggac gttatgatga
     5641 cgattaaagt tggaatcatt gggtgtggtg gtattgcgaa tggcaagcac atgccaagtt
     5701 tacaaaaagt tgaaaatgtt gaaatgatcg cattttgtga cgtagacatt tcgaaagcag
     5761 cgagtgcggc agaagcatac ggaactgaca atgcaaaggt ttatgatgat tacaaagcat
     5821 tgttaaaaga tgacacgatt gatgttatcc atgtttgtac gccaaatgac tcgcattgtg
     5881 aaattactgt agcagggttg catgccggta aacatgtgat gtgtgaaaaa ccaatggcta
     5941 aaacgacagc agaagctcaa aaaatgatag atacagctaa atcaacaggt aaaaaattaa
     6001 caataggtta tcaaaatcgc ttcagaccag atagtcaatt tttacatcaa gcagcgcaac
     6061 gtggcgactt aggagacatt tacttcggaa aggcacatgc cattcgtcgt cgagcagtac
     6121 caacatgggg tgtctttcta gacgaagaag ctcaaggtgg aggaccatta atcgatatcg
     6181 gtacacacgc tttagattta acgttatgga tgatggataa ttatgagcca gaatcagtga
     6241 tgggttcaac attccataaa ttaaataaac agcatcatgc ggcaaacgct tggggttcat
     6301 ggaatccaga tgaatttaca gttgaagatt ctgcgtttgg atttattaaa atgaagaatg
     6361 gagcgacgat cattttagaa tccgcttggg cgattaattc tttagaagtg gatgaggcaa
     6421 aatgttcatt atcaggaacg aaagcaggtg ctgatatgaa agatggtcta cgtattcatg
     6481 gtgaagacat gggcacactt tataccaaac acgttgaatt ggaaagcaaa ggcgtcgact
     6541 tttatgaagg taatgaagtg gatgaagctg aagaagaagc aaaagcttgg attgatgcag
     6601 ttgtaaatga tactgaacca gttgtgaaac cggaacaagc aatggtagtt acaaaaattc
     6661 ttgaagcgat ttatcagtct gcaaaatcag gcaaagcaat ttactttgaa taacatcata
     6721 cggtaaggag gcacatcatg acaaaattaa aagttggtgt gataggtgtt ggtggtattg
     6781 cacaagaccg tcatattcca gcattgctga aactcaaaga cacagtctca ttagttgcag
     6841 tacaagatat taatacagtg cagatgattg atgttgcgaa gcgctttaat atacctcagg
     6901 cagttgagac acctagcgag ctgtttaaac ttgttgatgc ggtggtcatt tgtacaccca
     6961 ataaattcca tgctgatctt tctatagaag cattgaacca tggtgtccat gtcttatgcg
     7021 aaaagccaat ggcaatgacg acggaagagt gtgatcgcat gattgaagcg gctaataaaa
     7081 atcacaaatt attaactgtc gcttatcatt atcgtcacac agatgtcgct atgactgcta
     7141 aaaaagcaat tgaagcaggt gtggttggta aaccattagt agcacgtgta caagcgatgc
     7201 gtaggcgtaa agtacctggg tggggcgttt ttaccaataa agcgttgcaa ggtggcggta
     7261 gtttaatcga ttatggttgc cacttgttag acttatcttt gtggctatta ggtaaagata
     7321 tggtgccgca tgaagtgcta ggaaaaacat ataatcaatt gagcaaacaa ccgaatcaaa
     7381 ttaatgattg gggaacattt gatcacacta aatttgatgt cgatgatcat gttactagtt
     7441 atatgacatt tgccaatcga gcaagcatgc agtttgaatg ttcgtggtct gcaaatataa
     7501 aggaagataa ggtacacgtt agtttatcag gagaagatgg cggtatcaat ttatttccat
     7561 ttgaaatata tgagccccgc tttggaacta tttttgaaag caaagctaat gttgagcata
     7621 acgaagacat tgctggtgag agacaggcgc gtaactttgt caatgcgtgt ttaggtatag
     7681 aagagattgt ggtgaaaccg gaagaagcac gcaatgtaaa tgcccttata gaagcgattt
     7741 atcgtagtga tcttgataat aagagtatac aactttaatg attatcatat atgatacaaa
     7801 attctcaata taaaaagaag gagtgctttt caatgaaaat aggtgtattt tcagtattat
     7861 tttacgataa aaattttgaa gatatgttag attatgtcgc agaatctgga ttggatatga
     7921 ttgaagttgg aacaggtggt aacccaggag ataaattttg taagttagat gagttgttag
     7981 aaaatgaaga caagcgacaa gcatttatga agtcaatcac agacagaggc ttacaaataa
     8041 gtggtttcag ttgtcataac aatccaattt ctccagatcc gatagaagcg aaagaagccg
     8101 atgaaacgtt acgtaaaaca atccgtttag caaatctatt agacgtgcca gttgttaata
     8161 cattttctgg cattgcaggg tcagatgata ccgctaaaaa gcctaattgg cctgttacac
     8221 cttggccaac agcctactct gaaatttatg attatcagtg gaatgaaaag ttgataccat
     8281 attggcaaga tttagctgag tttgcaaaag agcaagatgt aaaaattgcc atagagttgc
     8341 atgcaggatt tttagtgcat acaccatata caatgttgaa gttacgtgag gctacaaatg
     8401 aatatatcgg tgctaactta gatcctagtc atttatggtg gcaaggtatt gacccaattg
     8461 ctgcgattcg catattaggc caagcaaatg caattcatca cttccatgct aaagatacgt
     8521 atattaatca agaaaatgta aatatgtatg gtctaactga tatgcaacca tatggtaacg
     8581 ttgcgacaag agcatggaca ttccgtacag ttggttatgg acatagtcca tatgtatggg
     8641 cagatatcat aagtcaactt attattaatg gatatgatta tgtattaagt attgaacatg
     8701 aagatcctat tatgtcagta gaagaaggtt tccaaaaagc ttgtcaaact ttgaaatctg
     8761 ttaatattta cgacaagcca gcagacatgt ggtgggcata atacgaactc gaggttagtc
     8821 tgaagtttgt ctgaagtaag actgatggca gtgttgaatg aatgcatatg tcgccaagcc
     8881 attgccaaaa atttcacacc ttaaatcatg tcattgtttg taaagaaggt gtactttata
     8941 taagtgttta gcgatggtca tacccattca cagtaacaat cctcaccatt gaaaagagta
     9001 tataaccttt tcgatagtga ggtatatgat aataaaaaaa gcctgttgtc acaatggtca
     9061 tagacacgac atactttaaa ggtttctgaa tataatattt cagaacgcac tttaaagatg
     9121 gacgtcgatg tcgactaatg tgatgacagg ctttcatctt tttaaatatt cattaatttc
     9181 tcttcttgtt taatacgtac atataagaaa tacgcatacg gtactaataa aatagttgta
     9241 tatgttgcgt gtgttaataa taatacaccg attaattcag gaatgatgtt taagaagtaa
     9301 tttgggtgtt ttgtaatttt atataatcca gatttaataa taggatggtt aggtaaaatg
     9361 aataatttta atgtccaaat accacctaaa gttttaataa ccataaataa catgatataa
     9421 gcaaagatta atataactaa gccaatacca tttgcaaagc taaatgtatc tttattaata
     9481 aatgcctcta caccagccaa tacataaatt aaaacgtgtg ttattgctaa aaacttcgaa
     9541 tttttaacgc catattcaac tgcaccgtct gcttttaatt gttttgagtg attaatagat
     9601 atctttaagc tgacaagtct gatacagaaa aagataagta atatagatag aatcatgatg
     9661 tcctccgtca ttatgtcata tgtataaacg ttgattttga caacataaag tattttatag
     9721 ataaaacttg tcaaatacta ttaactattt attaatttta gtacataaaa atgtttctaa
     9781 gtatgtgttt atgttcagta ttttggataa tttaataatt ttaaggatat taagcgctta
     9841 caccgacgtg atatatttgg cttaacgaaa atgattgagg tgacagagat gaactttttt
     9901 gatatccata agattccgaa caaaggcatt ccattatcgg tacaacgtaa attatggctt
     9961 agaaacttta tgcaagcttt ctttgtagtg ttctttgttt atatggctat gtatttaatt
    10021 cgaaacaact ttaaggcggc acaaccgttt ttaaaagagg aaattggatt atctacatta
    10081 gaacttggtt atatcggatt agcatttagt atcacgtacg gtttagggaa aacattactt
    10141 ggatattttg tcgatggacg taacacaaaa cgtattatct cgttcttact tatcttatct
    10201 gcgattacag ttttaattat gggatttgtt ttaagttact ttggttctgt aatgggatta
    10261 ttaattgtac tttggggact taacggggtg ttccaatcag ttggtggacc tgcaagttat
    10321 tcaacgattt caagatgggc gccaagaacg aaacgtggcc gatacttagg attttggaat
    10381 acatcacata atatcggtgg tgccattgca ggtggtgttg cactttgggg tgctaatgta
    10441 ttcttccatg gaaatgttat agggatgttc attttcccat cggtgattgc attacttatt
    10501 ggtatcgcaa cattatttat cggaaaagat gatccagaag aattaggatg gaatcgtgct
    10561 gaagaaattt gggaagagcc ggtcgataaa gaaaatattg attctcaagg tatgacgaaa
    10621 tgggagatct ttaaaaaata tatcctggga aatcctgtta tatggattct atgtgtttca
    10681 aacgtctttg tatacattgt acgaatcggt attgataact gggcaccgtt atatgtgtca
    10741 gagcatttac actttagtaa aggcgatgca gttaatacga tattctactt tgaaattggt
    10801 gcattagttg caagtttatt atggggctac gtatcagact tattaaaagg tcgtcgtgca
    10861 attgtagcta ttggctgtat gtttatgatt acatttgttg tcttattcta cacaaatgct
    10921 acaagtgtaa tgatggttaa catttcattg tttgcattag gtgcgttaat ctttggtccg
    10981 caattattaa ttggtgtatc attgactggt tttgttccta aaaatgccat cagtgtagca
    11041 aacggaatga caggttcatt cgcgtatcta ttcggtgact caatggcgaa agttggtttg
    11101 gcggctattg ctgatccaac acgtaacggt ttaaacatct ttggatatac attaagtgga
    11161 tggacagatg ttttcatcgt cttctatgtt gcattattcc taggcatgat tctattagga
    11221 atcgttgctt tctatgaaga aaagaaaatt agaagtttaa aaatctaata tcaatcggat
    11281 taaaagtatc gccaatctat tgcaatatag ttggcaatcc tgccccgacg gcatgtgcgt
    11341 gaagagatga aagatactgc ttctaccctt gcaaatatat catctctatg tctcggggca
    11401 gatcataatt ccctgttatg aagtatcctt atttgcccga cttagggtga ctcaatgaat
    11461 ttactcctta caataaagac atatagcggt gtcgatattg tagggagtat tgttttatat
    11521 ttaaactctc taaaaagcgg actgaaagaa aagtgaaaac ttctctatca gtccgctttt
    11581 tcatagaaca aaatgtaggc gccataatca ttagttattt gctaatctat ttagcttgct
    11641 tacaataatc acttggcgac atttgtaaat attttttaaa atgatagcta aacattttat
    11701 actctgaaaa gcctactttg tctgcaattt catagtgttt gtaatgtcga tctaacaatt
    11761 gcagagattg taaaatacga tagcgattta aataatcgac aattgtaata ccaacatgat
    11821 ctttaaatgt tcgcatcgca tacgattcac taacatcgat atgttgaatt aaatctgaaa
    11881 cagtcacttt cgtttgataa gattgcttaa tttgatcaac aatctgattt acataataat
    11941 catcgtattc tacttttaat agtggttgga aggcatcatg acaagatgct aagctacggc
    12001 cgttctgtga ttgttgctct aataaggtac ggacaagtct tcctaaaata acttctaatt
    12061 gtgcatggtc tactggtttt aataaataat caagaacatg atgttgaatg ccggctttca
    12121 tatattcaaa gtcatcgtaa ctcgataata tgatgacatt acaatctaga tgcgcaatat
    12181 cattgagtaa atcgacgcca tttttacgtg gcatacgaat atcagtaatt actaattctg
    12241 gctgatgttg ttgaattagt gataatgctt caacaccatc tttagcagtg tatattgtat
    12301 tgaaatgata gtctccccaa ggaatgattt gctttaaacc ttctcgaata attcgttcat
    12361 catcacaaat aactacctta aacatctaca ttcccccttg aaagtggtat tttataacaa
    12421 attaacgtac cttgattacg ctttgaaaaa atatggagtc gtgcatgtga accatattga
    12481 atcattgctt tattgtgtaa atgatttaat cccaaatgct tagtatcaaa tacatcatta
    12541 ttaagagatt ggcgtacata ttgcaggcga gatgacgaca tcccgatacc attgtcgcaa
    12601 actaaaacat gtaagttctg acgtgccaat gtcaggcgta tagtaatgtc caatgactca
    12661 gtatctctac catgtttaat agcattttct atgagtggct gaagcatcat tttaccaatt
    12721 gtctggtgac gcgcttcttc agaactttcg atatggagct taatcatgtc atcaaaacgg
    12781 atgttttgta tcgcaacata ctgttcaatg tagttcaact cttcgtttaa ttccactgta
    12841 tgtgagtttg tacgtaatga gtaacgtaac atttgcgata attgttggac cacagtttgt
    12901 gctaatttcg gagataacgt aattaaatat tgtattgttt gcatcgtatt gaataggaaa
    12961 tgaggttgga attggcgttc tatttccttt aactgaatat cacgcaagcg acgttctgta
    13021 tgctcgatag aatggatcag ttgctcattt gattcaaata aatcgtaaat ataattatta
    13081 atttcttcta gttcactgtt gttttttaaa ggcgtatatg tacctagatg acgatttttg
    13141 gcatagtaaa ttttttgaat aatcgtttcg atatcttttg tttgtcgttt agccatatta
    13201 tctgcgctaa tgaaaccaaa tattactagt aaaacaagaa ctacggccat aacaattaac
    13261 aacgtgatac catcttcaat gttttcatgt atatctttat aaataatgag acgatggtca
    13321 gcatggttta attttacaga ttcattcata aatccgaatt gttgtggtct atacttttca
    13381 cctatagtaa aacggtcatc attggcgtat aaaatattgt catattgatc aacgataagt
    13441 gcgaattgtc ggctatcttt cttaatttca attaaacgtg gggtgttagc catataaatt
    13501 ttaagcatat atgtactatt tttgaattta agctgatgcg ttgaaaataa atacatattt
    13561 ttagtgttta aatgttcata attattggtt ataaactgat ttggtccaga taattcataa
    13621 taaagtgttg cgggctgttg gtgtattaat tttaataatt cacgttttgt agcggtaaca
    13681 tcatgatgat ttgttaaatc gagctcttga aacgaattat tatgctgtgt aataaatgtc
    13741 tgaatctgct tttcagtgtg atgtaaagat gactgacttt catcaacatg ttgatgaatc
    13801 gtacgatgct ctatccaaat atagatggca tagaagctta ctagtccaat aataaggact
    13861 aaaaatactg gaaaaatagt agacgcaaat aacgatcgtc tcaattgatg tctataaggt
    13921 ttgtatgccg tcattgaatc atctccaaaa atttatgatg tggaatgtcc ggtaatttag
    13981 atttcggtat taaaggtatg ttcttaagat tttcgataga ctgatcgctt tgttcactaa
    14041 catcctttcg aattgacttg gcatcgaact ctgcaactaa tcgttgttgt actgagcggc
    14101 ttgttaaata ttgcactaac tttttacgct taggatgagg gtgtgcattt ttaactaaag
    14161 caataccatc gacatttaac attgttcctt caataggata aacgattgat acaggataac
    14221 ctttgttttt ccatgtgcgt gcatcttgtt cgtagcttag acctgcgtaa tatttacctt
    14281 tagcaacatc ttcaatgact ttagacgtct ttgacagttg catcgcatgg tcttggaatt
    14341 gatgcacatc acttactcga tgatgcatgc tataaatagc acgcatatgt tgatagcctg
    14401 tcgttgttgt atttggattt gagtacgcaa ttttaccttt aagtataggt tttaataaat
    14461 cttgataacc tcgaatcgta atatctcctt gtaaatctga attaactact ataactgttg
    14521 gcattaatag aaaactagta acatatttat tgttcgagcg ataatcctct aattgctgtg
    14581 ttacagatgt atcttgatag ggaacaaaat cttctggatg atcaattgtt tctgacaaca
    14641 caccacccat aaagacatca ccacgctccg aaagatcttc attatgcaag tttgaaagca
    14701 gtacttgagt agatccatgt ttgatttcaa ttttgacatg ctcttgtttt tcaaaatcat
    14761 ttaaaattgg acgaatcaag tttgattgat acggagaata aactgttaat acatttttat
    14821 cggattcaga gtgacgcgta ttagcgcatg ctgataaaaa aatgagaaat aatagcaaga
    14881 tataaatttt tgatttcatg atatcccatc aattctatgt atattttaat acaataattt
    14941 tagcaataaa tgacgcataa gtaatgttaa atatttagaa atgtttatag atgacttgtt
    15001 aagacgttgc aaatgttgtg atagcaaaaa atttttgttt gtcaagacga tttaccgagg
    15061 ctgtaaaatc aaactgttat attttatttg tagctgttat ataaaaatcg gcaagatatt
    15121 gaacggttca aaagtgaatt tttacgtcaa taaaagtatt taatccagtc tcttcatata
    15181 taaaagtaaa tctttctaag tgttgattta acgcttat
//
LOCUS       DAHHOJ010000067        15140 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.067, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000067 DAHHOJ010000000
VERSION     DAHHOJ010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15140)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15140)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15140
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.067"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(77..538)
                     /locus_tag="NBH32_001717"
     CDS             complement(77..538)
                     /locus_tag="NBH32_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF420 domain-containing protein"
                     /protein_id="HCD1515310.1"
                     /translation="MGVPILPTISTTCIVISAILIAIGWRLIWKREINKHKNVMLAAA
                     VFALTFFLIYASRTIFIGNTAFGGPASIKKYYTIFLFFHINLATIGGILGLVQIITAF
                     KDKYNVHRKFGPFASVIWFCTAITGVAVYLLLYVLYPGGETTSLIKATFGH"
     gene            complement(563..1474)
                     /locus_tag="NBH32_001718"
     CDS             complement(563..1474)
                     /locus_tag="NBH32_001718"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCD1515311.1"
                     /translation="MSKEHTLSQNISRVNFKELQQIIKMGLVQGNLIPAFAGAWLAVV
                     MTNHSFLSSIPQILLMLLGSTLIMGGACALNNYYDQDIDRIMPSKQNRPTVNNRITDQ
                     NLLLLSFGMMLVGEICLFLLNIPSGVLGLMGIVGYVSYYSIWSKRHTTWNTVIGSFPG
                     AVPPLIGWVAIEGQISLTAIALFLVVFCWQPIHFYALAIKRKDEYALANIPMLPSVKG
                     FKRTRVSMFIWLIILLPVPLLLINLGVVFVVLATLLNLGWIALGLTTFKKNSDQTKWA
                     TQMFIYSLNYLVIFFVLAVIVSLLTLI"
     gene            1913..2824
                     /locus_tag="NBH32_001719"
     CDS             1913..2824
                     /locus_tag="NBH32_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000467197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme A synthase"
                     /protein_id="HCD1515312.1"
                     /translation="MFGKKNLKWLGVVATLMMTFVQLGGALVTKTGSADGCGSSWPLC
                     HGALIPEFFPIDTIIELSHRAVSALSLLMVLWLVITAWKHIGYIKEIKPLSIISVGFL
                     LLQALIGAAAVIWQQNDYVLALHFGISLISFSSVFLITLIIFSIDQKYEADELYIKKP
                     LRRLTWLMAIIIYCGVYTGALVRHADASLAYGGWPLPFHDLVPHSEQDWVQLTHRIMA
                     FIVFTIIMITYIHAVKNYPNNRTVHYGYTAAFILVILQVITGALSIMTNVNLIIALFH
                     ALFITYLFGMTTYFIMLMLRSVRSDKQ"
     gene            complement(2964..6416)
                     /locus_tag="NBH32_001720"
     CDS             complement(2964..6416)
                     /locus_tag="NBH32_001720"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HCD1515313.1"
                     /translation="MKQIKKLLVANRGEIAIRIFRAAAELDISTVAIYSNEDKSSLHR
                     YKADESYLVGSDLGPAESYLNIERIIDVAKQANVDAIHPGYGFLSENEQFARRCAEEG
                     IKFIGPHLEHLDMFGDKVKARTTAIKADLPVIPGTDGPIKSYELAKEFAEEAGFPLMI
                     KATSGGGGKGMRIVREESELEDAFHRAKSEAEKSFGNSEVYIERYIDSPKHIEVQVIG
                     DEHGNIVHLFERDCSVQRRHQKVVEVAPSVGLSPTLRQRICDAAIQLMENIKYVNAGT
                     VEFLVSGDEFFFIEVNPRVQVEHTITEMVTGIDIVKTQILVAAGADLFGEEINMPQQK
                     DITTLGYAIQCRITTEDPLNDFMPDTGTIIAYRSSGGFGVRLDAGDGFQGAEISPYYD
                     SLLVKLSTHAISFKQAEEKMVRSLREMRIRGVKTNIPFLINVMKNKKFTSGDYTTKFI
                     EETPELFDIQPSLDRGTKTLEYIGNVTINGFPNVEKRPKPDYELASIPTVSSSKIASF
                     SGTKQLLDEVGPKGVSEWVKKQDDVLLTDTTFRDAHQSLLATRVRTKDMINIASKTAD
                     VFKDGFSLEMWGGATFDVAYNFLKENPWERLERLRKAIPNVLFQMLLRASNAVGYKNY
                     PDNVIHKFVQESAKAGIDVFRIFDSLNWVDQMKVANEAVQEAGKISEGTICYTGDILN
                     PERSNIYTLEYYVKLAKELEREGFHILAIKDMAGLLKPKAAYELIGELKSAVDLPIHL
                     HTHDTSGNGLLTYKQAIDAGVDIIDTAVASMSGLTSQPSANSLYYALNGFPRHLRTDI
                     EGMESLSHYWSTVRTYYSDFESDIKSPNTEIYQHEMPGGQYSNLSQQAKSLGLGERFD
                     EVKDMYRRVNFLFGDIVKVTPSSKVVGDMALYMVQNDLDEQSVITDGYKLDFPESVVS
                     FFKGEIGQPVNGFNKDLQAVILKGQEALTARPGEYLEPVDFEKVRELLEEEQQGPVTE
                     QDIISYVLYPKVYEQYIQTRNQYGNLSLLDTPTFFFGMRNGETVEIEIDKGKRLIIKL
                     ETISEPDENGNRTIYYAMNGQARRIYIKDENVHTNANVKPKADKSNPSHIGAQMPGSV
                     TEVKVSVGESVKANQPLLITEAMKMETTIQAPFDGVIKQVTVNNGDTIATGDLLIEIE
                     KATD"
     gene            complement(6969..8195)
                     /gene="ftsW"
                     /locus_tag="NBH32_001721"
     CDS             complement(6969..8195)
                     /gene="ftsW"
                     /locus_tag="NBH32_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCD1515314.1"
                     /translation="MKNFRSILRYIGKTSKFIDYPLLVTYIVLSLIGLVMVYSASMVP
                     ATKGTLTGGIDVPGTYFYNRQLAYVIMSFMIVFFIAFLMNVKLLSNIKVQKGMIITIV
                     SLLLLTLVIGKDINGSKSWINLGFMNLQASELLKIAIILYIPFMISKKMPRVLSKPKL
                     ILSPIVLALGCTFLVFLQKDVGQTLLILIILVAIIFYSGIGVNKVLRFGIPAVLGFLV
                     VFVIALMAGWLPSYLTARFSTLTDPFQFESGTGYHISNSLLAIGNGGVFGKGLGNSAM
                     KLGYLPEPHTDFIFAIICEELGLIGGLLVITLEFFIVYRAFQFANKTSSYFYKLVCVG
                     IATYFGSQTFVNIGGISATIPLTGVPLPFISFGGSSMISLSIAMGLLLIVGKQIKVDQ
                     QRKKQQQKVDIRRQFN"
     gene            complement(8509..8784)
                     /locus_tag="NBH32_001722"
     CDS             complement(8509..8784)
                     /locus_tag="NBH32_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HCD1515315.1"
                     /translation="MAKQTTMKNAALKQLTKDADEILHLIKVQLDNLTLPSCPLYEEV
                     LDTQMFGLQKEVDFAVKLGLVDREDGKQIMLRLEKELSKLHEAFTLV"
     gene            8925..9407
                     /locus_tag="NBH32_001723"
     CDS             8925..9407
                     /locus_tag="NBH32_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515316.1"
                     /translation="MIQIKGAVKFPITLDSTTWIFDDRKVSIEDLEKGVFDGSKPIDF
                     EDNHEWNRAILEGQTNPPTLNSEIKYKKRAMLEGSFVINMTPFFKHSEPHDSATTIRL
                     SNEHDHLDIPIDLLPYLFFQFAKDGKRLYSDNGVDSFIYNQEDGYSYRINHVTHIEVI
                     "
     gene            9409..9576
                     /locus_tag="NBH32_001724"
     CDS             9409..9576
                     /locus_tag="NBH32_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaI family protein"
                     /protein_id="HCD1515317.1"
                     /translation="MRQVQCIICDAKVFIDERTTESKRLKNNPIRTFMCDDCKSRLDT
                     PKQRAQHYPLD"
     gene            complement(9716..11563)
                     /gene="typA"
                     /locus_tag="NBH32_001725"
     CDS             complement(9716..11563)
                     /gene="typA"
                     /locus_tag="NBH32_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HCD1515318.1"
                     /translation="MTNKREDVRNIAIIAHVDHGKTTLVDELLKQSGIFRENEHVDER
                     AMDSNDIERERGITILAKNTAVDYKGTRINILDTPGHADFGGEVERIMKMVDGVVLVV
                     DAYEGTMPQTRFVLKKALEQNLKPVVVVNKIDKPSARPEGVVDEVLDLFIELEANDEQ
                     LEFPVVYASAVNGTASLDPEKQDDNLQSLYETIIDYVPAPIDNSDEPLQFQVALLDYN
                     DYVGRIGIGRVFRGKMRVGDNVSLIKLDGTVKNFRVTKIFGYFGLKRLEIEEAQAGDL
                     IAVSGMEDINVGETVTPHDHQEALPVLRIDEPTLEMTFKVNNSPFAGREGDFVTARQI
                     QERLNQQLETDVSLKVSNTDSPDTWVVAGRGELHLSILIENMRREGYELQVSKPQVII
                     KEIDGVMCEPFERVQCEVPQENAGAVIESLGARKGEMVDMTTTDNGLTRLIFNVPARG
                     MIGYTTEFMSMTRGYGIINHTFEEFRPRIKAQIGGRRNGALISMDQGSASTYAILGLE
                     DRGVNFMEPGTEVYEGMIVGEHNRENDLTVNITKTKHQTNVRSATKDQTQTMNRPRIL
                     TLEEALQFINDDELVEVTPESIRLRKKILNKNVREKEAKRIKQMMQENE"
     gene            11665..11856
                     /locus_tag="NBH32_001726"
     CDS             11665..11856
                     /locus_tag="NBH32_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaF family protein"
                     /protein_id="HCD1515319.1"
                     /translation="MKQKKSKNIFWVFSILAVIFLVLFSFAVGASNVPMMILTFILLV
                     ATFGIGFTTKKKYRENDWL"
     gene            complement(12010..12837)
                     /locus_tag="NBH32_001727"
     CDS             complement(12010..12837)
                     /locus_tag="NBH32_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD1515320.1"
                     /translation="MALYGFAQGLIQEAGIRIKQLMEQNLTIETKSNPNDLVTNVDKA
                     TEDFIFDTILETYPNHQVLGEEGHGHDIDTSKGTVWVVDPIDGTLNFVHQQENFAISI
                     GIYIDGKPYAGFVYDVMADVLYHAKVGEGAYRGSQPLKPLNDSNLRQSIIGINPNWLT
                     KPILGEIFKEIVNDSRSARAYGSAALEIVSVATGNLEAYMTPRLQPWDFAGGLVILYE
                     VNGQASNLLGEPLTIGGPNSVLVGNRGVHREILDVYLEPHRDALIQLHERRFKRKSK"
     gene            12994..13608
                     /locus_tag="NBH32_001728"
     CDS             12994..13608
                     /locus_tag="NBH32_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YktB family protein"
                     /protein_id="HCD1515321.1"
                     /translation="MTKYTFKPKDFKAFNVEGLDARMEALNEYIRPQLHELGEYFSDF
                     FTSQTGETFYPHVAKHARRSVNPPKDTWVAFATSKRGYKMLPHFQIGMFEDQLFVMFG
                     IMHEAKDKATRAKVFERKFKAIQQLPDDYRVCLDHMKPDKPFIKDLTDDDLKEAIQRA
                     INVKKGEFFIARAITPQDKRLKSDKAFIAFLEETFDQFLPFYSA"
     gene            13792..>15140
                     /locus_tag="NBH32_001729"
     CDS             13792..>15140
                     /locus_tag="NBH32_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCD1515322.1"
                     /translation="MNNKRHSTNEQLSLDEINNTIKFDHRSSNKQKFLSFLGPGLLVA
                     VGYMDPGNWITSMQGGAQYGYTLLFVILISSLSAMLLQSMTVRLGIATGMDLAQMTRH
                     YLSRPIAIIFWIIAELAIIATDIAEVIGSAIALNLLFNIPLIVGALITVLDVFLLLFI
                     MKYGFRKIEAIVGTLIFTVLFIFIFEVYISSPQLNAVLNGFIPHSEIITNNGILYIAL
                     GIIGATIMPHNLYLHSSIVQSRTYSRHNNEEKAQAIKFATIDSNIQLSIAFVVNCLLL
                     VLGASLFFNSNADDLGGFYDLYHALKTEPVLGATMGAIMSTLFAVALLASGQNSTITG
                     TLAGQIVMEGFLRLHIPNWLRRLITRSLAVIPVIVCLIIFKGNAAKIEQLLVFSQVFL
                     SIALPFCLIPLQLATSNKDLMGPFYNKTWVNIISWTLIIILSILNVYLIVQTFQELQG
                     "
BASE COUNT         4964 a         2725 c         2222 g         5229 t
ORIGIN      
        1 ctgtccgact attcttcatc aaaaaacctg ataaaacaaa ttgccttatc agattagtat
       61 catttgtata agcatattaa tgaccaaatg ttgctttaat cagtgatgtc gtttctccac
      121 ctggatataa tacatataat aataagtaaa ctgctacacc tgtaattgca gtacagaacc
      181 atataactga agcgaatggc ccgaatttgc ggtgtacatt atatttatct ttaaatgcag
      241 taataatttg aactagacct agaataccac caattgttgc taaattaatg tggaaaaata
      301 agaaaatcgt ataatatttc ttaattgatg ctggtccgcc aaatgctgta ttaccgataa
      361 aaatcgttct acttgcatag attaagaaaa aagttaaagc aaaaacagct gcagctaaca
      421 taacattttt gtgtttattt atttcccttt tccaaataag tctccaacca atggcaatta
      481 aaattgcact aatgacaata catgtcgtac taatcgttgg taaaattgga acgcccatat
      541 ttttcatcct aacttaatta atctagatca aagtaagtaa tgaaacaatc acagctaaca
      601 cgaaaaagat cactaaataa tttagtgaat atataaacat ttgtgttgcc cattttgttt
      661 gatctgaatt tttcttaaat gttgttaaac ctaatgcaat ccatcctaaa tttaataatg
      721 tagctaacac tacgaatacg acacctaaat ttattagtaa taaaggtact ggcaataaaa
      781 taatcaacca gataaacata ctgacacgtg tacgtttaaa gcccttaact gatggtaaca
      841 ttggaatatt tgcaagtgca tattcatctt tacgtttaat agctaaggca taaaaatgaa
      901 ttggttgcca acaaaataca actaaaaaca gcgcaatcgc tgttaaacta atttgtcctt
      961 caattgcaac ccatccaatt agtggtggta ctgctccagg aaaactccca atcactgtgt
     1021 tccatgttgt atgtctttta gaccatattg agtaataaga cacataacct acaatcccca
     1081 taagaccaag tacgcctgat ggtatattca ataaaaacaa acaaatttct ccaactaaca
     1141 tcataccaaa acttaatagt aataaatttt gatctgtaat tctattattt acagttggtc
     1201 tattttgttt actaggcata atacgatcaa tatcttggtc gtaataatta tttaacgcac
     1261 atgcgccacc cataattaaa gtagatccta atagcattaa taaaatttga ggtattgatg
     1321 ataagaagga atgatttgtc attacaactg ctagccatgc gcccgcaaaa gccggaatta
     1381 agttaccttg aacaagtccc attttaatta tctgttgcaa ttctttgaag ttaactctgc
     1441 taatattttg tgacaaagta tgctctttgc tcataatccc cctccttaaa tttgttcata
     1501 taagattatg atatcttaga ttgcataaaa agactaggtt taataaaatt aaaatgtgac
     1561 aaattaacga caagagaaaa tgtcaatttt gtgacacaaa taacatttaa tttattgcta
     1621 taatgtatat gttagaaaat tttaataagt agaatcatgc atctaaaaga gattaatatt
     1681 taagcttcaa atttgagtaa acgtggatta cataattatc ccaataaaaa atcattacga
     1741 ttaagttctt tttatgtcgt ccacatacaa tacttgtaaa attaaatcat atttcctgcg
     1801 ttggatccca tcttttcata tcctacaatc aggtcaattt atagtatcat ctcaaatccg
     1861 gctattaatt ctaattctca gtgatgcgtt tttttatgat ggggtgtata aattgtttgg
     1921 caaaaagaat ttaaaatggt taggtgtcgt agcaacgtta atgatgacat ttgtacaact
     1981 tggtggagcc ttagttacca aaaccggatc agctgatggt tgtggttctt cttggccact
     2041 atgtcatggt gcgttgattc cagaattctt tcctattgat acgattattg agttaagtca
     2101 tagagccgtt tcagctttgt ctttattaat ggtcttatgg ttagttatca ctgcatggaa
     2161 acatataggc tatattaaag aaattaaacc tttatcaatc attagtgttg gattcttatt
     2221 attgcaagca ttaatcggag ctgccgctgt tatttggcaa caaaacgatt acgttttagc
     2281 attgcacttt ggtatatcat taatcagttt ctcatctgta tttttaataa cattgattat
     2341 tttctctata gatcaaaaat atgaagctga cgaattatat atcaaaaagc cattaagacg
     2401 tttaacatgg ttaatggcaa tcatcattta ttgtggtgtt tatactggtg cactagtgag
     2461 acatgcggat gcaagtttag catatggtgg ttggccattg ccattccacg atcttgtacc
     2521 acattcagaa caagattggg ttcaactcac gcatcgtatc atggctttta tagtgtttac
     2581 gattattatg attacttata ttcacgctgt taaaaattat ccaaataacc gtactgttca
     2641 ttatggttat acagctgcat ttatacttgt tatattacaa gttatcacag gtgcattatc
     2701 tattatgaca aatgttaacc tgataatcgc acttttccat gcattattta tcacttattt
     2761 atttggtatg acaacatact ttatcatgct aatgttacga tcagtaagaa gtgacaagca
     2821 ataacaaaaa agcaaaccgt aattttaatg gcacgcccat taaaattacg gttttttata
     2881 tcaatattta aaattaaacc taagacatgt aaaaacgaga ttacacgtca attgttgtgt
     2941 aatctcgttt tattttaatc attttagtca gttgcttttt caatttcgat taataaatcg
     3001 cctgtcgcta ttgtgtcacc attatttaca gttacttgtt taatcacacc gtcaaatggt
     3061 gcttgaattg ttgtttccat tttcatagct tcagtaatta gcaacggctg gttagctttc
     3121 acagattcac ctacactaac cttgacttca gttactgaac ctggcatttg cgcacctata
     3181 tgacttggat tactcttatc tgcttttggt ttaacgttcg catttgtatg tacattttca
     3241 tctttaatgt aaatacgtct cgcttgacca ttcatcgcat agtaaatcgt tctattacca
     3301 ttttcatctg gttcactaat cgtttctagt ttaataatta atcgtttacc tttatcgatt
     3361 tcgatttcta ctgtttcacc attacgcatt ccaaagaaga atgtaggcgt atcaagtaac
     3421 gataagtttc cgtattgatt tctagtttga atatattgtt catatacttt tggatatagt
     3481 acataactaa taatatcttg ctccgtaaca ggaccttgtt gctcttcttc aagcaactca
     3541 cggacttttt caaaatcaac cggctctaga tattcacctg gacgagctgt tagtgcttct
     3601 tggcctttta aaataaccgc ttgtaaatct ttattaaaac catttacagg ttgtcctatt
     3661 tcgcctttga agaacgacac tactgattct gggaaatcta atttatagcc atctgtaatc
     3721 acggattgtt catcaagatc attttgtacc atataaagtg ccatatcacc aactacttta
     3781 gacgatggtg ttacttttac gatatcacca aataagaaat tcactctgcg atacatatct
     3841 ttaacttcat caaatctttc gcctaaacct aaacttttag cttgttggct taaattcgaa
     3901 tactgtccac caggcatttc atgttgataa atttcagtat tcggtgattt gatatcactt
     3961 tcaaagtctg aataataagt acgtacagtt gaccaataat gactaagtga ctccatacct
     4021 tcaatatcag ttctaaggtg gcgtgggaag ccattcaatg cataatataa cgaattggcg
     4081 cttggctgac ttgttaaacc actcattgaa gcaacagcag tatcaatgat atcgacacca
     4141 gcatctattg cttgtttgta tgttaataaa ccattaccac ttgtatcatg agtgtgaaga
     4201 tgaattggta aatctacagc tgattttaac tcaccaatca attcgtaagc ggctttaggt
     4261 tttaataagc ctgccatatc tttaatcgct aaaatatgga aaccttcacg ttctaactct
     4321 ttagctagtt tgacataata ctctaaagta taaatgtttg atcgctcagg atttaaaatg
     4381 tcacctgtat aacaaatagt accttcagag attttgcccg cttcttgtac tgcttcattg
     4441 gcaactttca tttgatctac ccagtttaat gaatcgaaaa ttctaaagac atctatgcct
     4501 gctttagcac tttcttgtac gaatttatga ataacattat caggatagtt tttataacca
     4561 actgcgtttg aagcacgtaa caacatttgg aataatacat ttggaatagc tttacgtaga
     4621 cgttcaagtc gttcccatgg gttttccttc aagaaattat atgccacatc aaatgtagca
     4681 ccgccccaca tttctagtga gaaaccatct ttaaatacgt ccgctgtttt ggatgcgata
     4741 ttaatcatat ccttagttct aactcgtgta gctaataatg attggtgtgc atctctaaaa
     4801 gttgtatctg ttagtaagac atcatcctgc tttttaaccc attcagatac accttttgga
     4861 cctacttcat caagcaattg tttcgtacca ctaaatgaag cgattttact tgaagataca
     4921 gttggaattg atgctaattc atagtctggt ttcggacgtt tctcaacatt tgggaaacca
     4981 ttaattgtta cattacctat atattctaat gttttagtac ctctatctag agacggctga
     5041 atgtcgaaaa gttctggtgt ttcttcaata aatttagttg tgtaatcacc acttgtgaac
     5101 tttttattct tcattacatt aattaagaat ggaatattag ttttaacacc acgaatacgc
     5161 atttctcgta atgaacgtac cattttttct tctgcttgtt taaatgatat cgcgtgtgta
     5221 gataatttta cgagtaatga atcataataa ggtgatatct cagcaccttg gaaaccatct
     5281 ccagcatcaa gacgtacacc aaagccaccg cttgaacgat aagcaatgat tgttccagta
     5341 tccggcatga aatcatttaa cggatcttct gttgtaatac gacattggat ggcatagccc
     5401 aatgttgtaa tatctttttg ttgcggcata ttaatttctt caccaaataa atcggcacct
     5461 gctgcaacta aaatttgtgt cttaacaata tcaattcctg ttaccatctc tgtaattgta
     5521 tgctctactt gtacacgagg gttaacttct ataaagaaga attcgtcacc agatactaga
     5581 aattcaacag tacctgcatt gacatattta atattttcca tcaattgaat tgcagcatca
     5641 caaatacgtt gacgtaatgt tggtgataat ccaactgatg gtgcaacttc tacaactttt
     5701 tgatgacgac gttgtactga acaatcacgt tcaaataagt gtacgatatt tccatgttcg
     5761 tcacctataa cttgtacttc aatatgcttt ggactatcaa tgtatctttc tatgtaaact
     5821 tcactattac caaatgattt ttcagcttct gattttgctc tatggaaagc atcttctaat
     5881 tcactttctt cacgaacgat tctcatacct ttaccgccgc caccacttgt ggctttaatc
     5941 attagcggga aaccagcttc ttctgcaaat tcttttgcta attcatatga tttaattgga
     6001 ccgtctgtac caggaataac tggtaaatct gccttgatag ccgttgtacg agctttaact
     6061 ttatctccaa acatatctaa atgttctaaa tgaggaccaa taaatttaat tccttcttct
     6121 gcacaacgac gcgcaaattg ttcattttca cttaaaaatc catagccagg atgaatcgca
     6181 tccacattcg cttgttttgc tacatcaatg atacgctcaa tatttaaata actttcagca
     6241 ggacctaaat cacttccaac taaataggat tcatctgctt tatatctatg taatgaactt
     6301 ttgtcttcat tcgaataaat tgcaactgtg ctgatgtcta attctgctgc cgctctgaat
     6361 atacgaattg caatttctcc acggttagca acaagtaact tttttatttg tttcaatagc
     6421 gatacactcc tcaaactatt agaattttct aactaattag ataataaaat tttatcttaa
     6481 agcgctctgt tttgctatag ttatgtttca aattttcaaa atttaacatt cttgagacaa
     6541 ttaaaacctc cgcttcaaaa ataataattt caaaaatgac tatgcaacaa caggtagttc
     6601 acgtttttgt tgtgaaacat tttcgatttc tacaactcta aaaaattaaa aataaaattg
     6661 caaaacatca acatttatta tcaatagcga taactttatc ttatcatcat gattctaatt
     6721 tcgccaccac atttagtaat ttttagtcat aaaatttagt tataattata cgttgtttcg
     6781 tttataaaat ttgataatca tgagtaatct cgtaatatca aaacaaaaag gaagttaagc
     6841 gttgtttggt tgcctaactt ccgttattta actcatcagt ccaatttaac aaagagtata
     6901 taaatagcca atgtttagca tcgatttatg acagaagtca tctcgtacta aatattggct
     6961 agtatttttt aattaaattg tcttcttata tcaacttttt gttgttgttt ctttcgttgc
     7021 tggtctactt tgatttgttt acctacaatc agaagtaatc ccatagcaat acttaaacta
     7081 atcattgatg atccaccaaa actgataaat ggcaatggca caccagttaa tggaattgtt
     7141 gccgaaatac cgccaatgtt tacaaacgtt tggcttccaa agtatgtggc aatcccaaca
     7201 cacacaagtt tataaaaata tgatgatgtt ttatttgcaa actgaaaggc acgatataca
     7261 ataaagaact ctaaagtaat aactagcaat cctccgatta aacctaattc ttcgcaaata
     7321 attgcaaaaa taaaatctgt atgtggttct ggtaaatagc ccaatttcat tgcactattt
     7381 cctaatcctt ttccaaatac gccaccgtta cctatcgcaa gcaatgaatt ggaaatatgg
     7441 tatccagttc ctgattcgaa ttggaatgga tctgttagcg tactaaatct ggctgttaaa
     7501 taacttggta accaaccagc cattaatgca atgacaaata ctactaagaa tcctagcact
     7561 gctggtatac caaatcttag gactttgttt accccaattc ctgaataaaa aatgatcgca
     7621 actaaaataa ttaatattag taatgtttgc cctacgtctt tttgtaggaa aactaaaaac
     7681 gtacaaccta atgctaatac aataggactt aaaattaatt ttggtttact taatactcta
     7741 ggcatttttt tactgatcat aaatgggata tataatatga ttgcaatttt taataactca
     7801 gatgcctgta agttcataaa tcctaagttt atccaacttt tagaaccatt aatatcttta
     7861 ccgattacta acgtcagtaa taatagcgag acgatagtta taatcatacc tttttgcact
     7921 ttaatattac tcagtaattt aacattcatt aaaaatgcaa taaaaaatac aatcataaaa
     7981 ctcattatga catatgctaa ttgtcggttg taaaaatacg ttcctggaac atcgatacca
     8041 ccagtcaatg tgcctttagt tgctggaacc atactggcac tatataccat cactaatcca
     8101 attaaactca atacaatata tgtaactaat aacgggtaat caataaactt tgaggtttta
     8161 ccaatatacc gtaaaatact tctaaaattc ttcatatatt cagtcatcca attctacaat
     8221 ttatacttca aatgatttcg ttttatactt taattttaaa aatccatgtt acttattata
     8281 tacatgacat cgtcataatc acaaatatta acaggtatct tgaggtaatt tatagaatat
     8341 ttgtttactt aatatcgtga attgctaaaa cccaataaaa gtgactgaca tgtcatcaag
     8401 aagacaacat aacagccact ttaatcagta attaaactat aaatataaaa tcggcgcagc
     8461 tcaattgtcg atatctcacg ccgttttaat atgcttatat attaaacctt aaacaagtgt
     8521 aaaagcttca tgtaattttg aaagttcttt ctcaagacgt aacattattt gtttgccatc
     8581 ttcgcggtca actaaaccta atttaacagc aaaatcaact tctttttgaa gtccaaacat
     8641 ttgtgtatct agtacttctt catataatgg gcatgaaggt aatgttaaat tatctagttg
     8701 aactttaatc agatgtaaga tttcatcagc atctttagtc aattgtttca aagctgcatt
     8761 tttcattgtt gtttgtttcg ccatgacacg ctccccctct atataaattc tcaagtctta
     8821 ctaaaagttt atcttactaa atattaaaaa gcaagtagat ttaaaatgtg tcgttagcaa
     8881 tttgtgttaa aataatgcta tatacattga tagggagtgg gaaaatgata caaattaaag
     8941 gagccgtaaa gtttcctata acattagata gtacgacttg gattttcgat gatagaaaag
     9001 ttagcatcga agatttagaa aaaggtgtat ttgatggttc aaaaccaatc gactttgaag
     9061 ataatcatga atggaatcgt gctatcctag aaggacaaac taatccgcct actttaaatt
     9121 cagaaataaa atataaaaaa cgtgccatgt tagaaggttc atttgtcatt aatatgacac
     9181 catttttcaa acattccgaa ccacatgact ctgcaactac tattcgatta tcaaatgagc
     9241 atgatcattt agatataccg atagatctat taccctattt atttttccaa tttgcaaaag
     9301 acggtaaacg actttacagc gataatggtg tagatagttt tatttacaat caagaagatg
     9361 gttattcata tcgaatcaac catgtaactc atattgaggt gatttaattt gagacaagtg
     9421 caatgcatta tctgtgatgc aaaagtattt atagatgagc gcacaactga gtctaaacga
     9481 cttaaaaaca atcctattcg aacatttatg tgcgatgatt gtaaaagtcg attagacaca
     9541 cctaaacaac gtgcgcaaca ctatccgctc gattaatatc tgaataaaca agttagaaat
     9601 ttgaaaatgt gaaagtaaca ggaaataata gcatttaaac actttttcaa atattatatg
     9661 ccaaaagaga agctaagcaa ctttcattgc tcagcttctc ttttattatg ttaaattatt
     9721 cgttttcttg catcatttgt ttgatacgct ttgcttcttt ttcacgaaca tttttgttta
     9781 aaattttctt tcttaaacgt atgctttctg gtgtaacctc aacaagttca tcatcattaa
     9841 tgaattgtaa cgcttcttcc aatgttagaa tacgcggtct attcattgtt tgtgtttggt
     9901 ctttcgttgc agaacgtacg ttagtttgat gttttgtttt agtgatgtta acagttaaat
     9961 cattttcacg attatgttca ccaacaatca taccttcata aacttcagta ccaggttcca
    10021 tgaagtttac acctctatct tcaagtccta aaatggcata agtacttgca gaaccttgat
    10081 ccattgaaat taatgcacca ttacgacgac cgccaatttg tgctttaata cgtggtctaa
    10141 attcttcaaa tgtatggtta ataataccgt aacctcttgt cattgacata aattcagtcg
    10201 tataaccaat cataccacga gccggtacat taaagattaa acgtgtaagt ccattatcag
    10261 ttgtagtcat atcaaccatt tcacctttac gtgcacctaa tgattcaata accgcacctg
    10321 cattttcttg tggcacttca cattgcacac gttcaaatgg ttcacacatt acaccatcaa
    10381 tttctttaat aattacttgt ggttttgaaa cttgtaattc ataaccttca cgacgcatat
    10441 tttcaataag gattgataaa tgcaattcac ctcgaccagc aactacccat gtatctggag
    10501 aatctgtgtt agaaactttc aaagatacat ctgtttctaa ttgttgattt aaacgttctt
    10561 gaatttgacg cgctgttaca aagtcacctt cacggccagc aaatggtgaa ttgttaactt
    10621 taaatgtcat ttcaagagta ggctcatcaa tacgtagaac tggcaatgct tcttgatggt
    10681 catgtggtgt tacagtttca ccaacattaa tgtcttccat acctgaaaca gcaattaaat
    10741 ctccagcttg tgcttcttca atttctaaac gttttaatcc aaagtaacca aagattttag
    10801 ttacacggaa gtttttcact gtaccgtcta atttaattag tgatacatta tctccgacac
    10861 gcattttacc tctgaataca cgaccaatac caatacgtcc aacataatca ttgtagtcca
    10921 acaatgctac ttggaattgt aatggctcat cactgttatc aattggagct ggtacataat
    10981 caataattgt ttcatataat gattgtaaat tatcatcttg tttttcagga tctaagctag
    11041 ctgtaccatt tactgctgaa gcataaacaa cagggaattc taattgttca tcgtttgctt
    11101 ctaattcaat aaataaatct aaaacttcat ctacaacacc ctctggacgt gctgatggtt
    11161 tatcaatttt attaacaaca acaacaggtt tcaggttttg ttctagcgct tttttaagta
    11221 caaaacgtgt ttgaggcatt gtaccttcat acgcatctac tactaagaca accccatcaa
    11281 ccattttcat aatacgttct acttctccac caaagtctgc atgtcctggt gtatccaaaa
    11341 tattaatacg tgtaccttta taatcaacag ccgtattttt ggctagaatc gtaattccac
    11401 gctctctttc aatatcgtta gagtccattg cacgttcatc gacatgttca ttttctctga
    11461 atataccaga ttgttttaac aactcatcta ctaaagttgt tttaccatgg tcaacgtgag
    11521 caataattgc tatattgcgg acatcttctc ttttattagt catataaata gtttccttct
    11581 ttcttaagta aaatcccact tttaattaca acttttttat tatatcatat aattgagaaa
    11641 agataagcaa aaggtgggga taggatgaaa caaaaaaaat ctaaaaatat cttttgggta
    11701 ttttctatat tagctgttat tttcttagta ttatttagtt ttgctgttgg tgcatcaaat
    11761 gtaccaatga tgattttaac atttatatta ctcgttgcaa cctttggaat tggatttact
    11821 acaaagaaaa aatatcgaga aaacgattgg ctataagtta tatcgacaat aacacgcgtt
    11881 gattaaagac cacgcatgtt catgatgaac atttaaatgg ttttatattg gcgcgtgttt
    11941 ttattattta agatataaaa gcgtaatatg ttacctaatt gttaggacta catattacgc
    12001 ttttggtaat tattttgatt ttcttttaaa tcgtcgttca tgtaattgta ttaacgcatc
    12061 gcgatgtggc tctaaatata catcgaggat ctctctatga acaccacgat taccaactaa
    12121 aactgagttt ggaccaccaa ttgttagcgg ttcacccaat aaatttgaag cttgtccatt
    12181 tacttcatat aaaataacca atcctccagc aaaatcccac ggttggagtc taggtgtcat
    12241 atatgcttct aaattaccgg tagcaacaga aacaatttca agcgctgcac taccatatgc
    12301 ccttgcactt ctagaatcat taacaatttc tttaaagatt tctcctaaaa ttggtttagt
    12361 taaccagttc ggattgatcc caataatgct ttgtcttaga ttagaatcat tcaatggttt
    12421 caagggttgg ctacctcgat atgcaccttc ccctacttta gcatgatata agacatcagc
    12481 cataacatca tatacaaaac ctgcataagg tttaccatcg atataaatac caattgaaat
    12541 cgcgaaattt tcttgttgat gaacaaaatt caatgtaccg tctattgggt caacaaccca
    12601 taccgtacct ttggaagtat cgatgtcatg accatgccct tcttcaccta atacttgatg
    12661 attgggatat gtttctaaaa ttgtatcaaa aataaaatct tctgttgctt tatctacatt
    12721 tgtaacaagg tcattcggat ttgactttgt ttcaattgtt aaattttgct ccatcaattg
    12781 tttaattcta attcctgctt cttgaataag tccttgggca aatccataaa gtgccataac
    12841 atcatctcct aacatcttta ttatacatca acattttata aaaataactt cttatgataa
    12901 aaatgaaaat actaatttta atgagagcgt cttaagccgc aattgataaa acatatgcta
    12961 caatattttt aaacactata caggaggtac tctatgacaa aatatacatt taaacctaaa
    13021 gatttcaaag cgttcaacgt agaaggttta gacgcacgaa tggaagcttt aaacgaatac
    13081 atacgaccac aactccatga attaggagaa tattttagtg acttctttac aagtcaaact
    13141 ggtgaaacat tttatcctca cgtagcaaaa catgctagaa gaagtgtgaa tcctcctaaa
    13201 gatacatggg ttgcttttgc tacaagcaaa cgaggctata aaatgttacc tcatttccaa
    13261 attggtatgt ttgaagatca actgtttgtt atgtttggaa tcatgcatga agcaaaagat
    13321 aaagcaacac gtgcaaaagt ttttgaaaga aaatttaaag ctattcaaca attacctgat
    13381 gattatcgtg tttgcttaga tcatatgaaa cctgataaac catttattaa agatttaacg
    13441 gatgatgatt taaaagaagc gatacaaaga gccatcaatg taaaaaaagg tgaattcttt
    13501 atagcgcgtg caatcacacc acaagataaa agattaaaaa gtgacaaagc atttattgca
    13561 tttttagaag aaaccttcga tcagttctta ccattttatt ctgcataaat aactttgttt
    13621 aaataataga gcactaaatc acatccatga tttcgtgctc ttttttctta atattaaatc
    13681 gaacgttcaa cataataatt catactttta aaaaaattaa aataatttta ggttgaccta
    13741 atcattttat taggttatta tattgtccat aagaagtaga ggtgagtcaa aatgaataat
    13801 aaacgacatt caacaaatga acaattaagt ttagacgaaa taaacaatac aattaaattc
    13861 gatcatcgca gttcaaataa acagaaattt ttatcatttc ttgggcctgg gttattagtc
    13921 gctgttggtt acatggatcc cggaaactgg ataacatcaa tgcaaggtgg cgcgcaatat
    13981 ggctatactt tgctattcgt aattcttatt tcaagtttat cagcaatgtt acttcaaagc
    14041 atgacagtga gattaggaat agcaacaggt atggacttag ctcaaatgac acgacattat
    14101 ttatcaagac ctattgctat aatcttttgg attattgcag aactagcaat tatcgccaca
    14161 gatattgctg aggttattgg tagtgctatt gctcttaatc tcctatttaa cataccttta
    14221 atcgtcggtg cactaataac tgtacttgat gtatttttac tactatttat aatgaaatat
    14281 ggttttagaa aaattgaagc tattgttggt acattaattt tcacagtgtt attcatcttt
    14341 atatttgaag tctatatttc atcaccacag ttgaacgctg tgttaaatgg atttatacca
    14401 catagtgaaa tcattacaaa taacggcatt ctctatattg cattaggtat tattggcgct
    14461 acaattatgc ctcataattt gtacttacat tcatcaattg tacaatctag aacatactca
    14521 agacataaca atgaagaaaa ggcgcaagcg attaaatttg ctacgataga ttcgaacatt
    14581 cagttatcaa tcgcatttgt agtcaattgc ttattattag tgttaggagc atcactattt
    14641 ttcaactcga acgctgacga tttaggtggt ttctatgatt tatatcacgc cttaaaaact
    14701 gaacctgtac taggtgcaac aatgggtgca atcatgagta cattatttgc agttgcatta
    14761 ttagcgtcag gtcaaaattc aacgattact ggtactttag caggacaaat tgtaatggaa
    14821 ggatttttaa gattgcacat accaaattgg ttaagacgtt taattacacg ttcccttgct
    14881 gtcattcctg ttatcgtatg cttaatcatt tttaaaggta atgcagctaa aattgagcaa
    14941 ctacttgtat tctctcaagt atttttaagt attgctctgc cattctgttt aataccatta
    15001 caattagcta ctagtaataa agacttgatg gggcctttct ataataaaac atgggttaat
    15061 atcatttcat ggacacttat tattattcta agtattttaa atgtctattt aatcgttcaa
    15121 acattccaag aactacaagg
//
LOCUS       DAHHOJ010000068        15059 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.068, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000068 DAHHOJ010000000
VERSION     DAHHOJ010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 15059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15059
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.068"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            161..556
                     /locus_tag="NBH32_001730"
     CDS             161..556
                     /locus_tag="NBH32_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl peptide receptor-like 1 inhibitory
                     protein"
                     /protein_id="HCD1515323.1"
                     /translation="MKKNITKVIIASTVIATGLLTHSNDAKAFFSYEWKGLEIAKKLA
                     DDEKKDEERADRLIKDAEKERTYTGKTVEDLYVIAKKLGKGNTIAVVRIIDGGKNGYY
                     TFDITRPLEEHRKNIPVVKNGEIDSITWY"
     gene            537..608
                     /locus_tag="NBH32_001731"
     CDS             537..608
                     /locus_tag="NBH32_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515324.1"
                     /translation="MQLLGIKKHTELNSCTPNVRKKC"
     gene            complement(916..1245)
                     /gene="ecb"
                     /locus_tag="NBH32_001732"
     CDS             complement(916..1245)
                     /gene="ecb"
                     /locus_tag="NBH32_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement convertase inhibitor Ecb"
                     /protein_id="HCD1515325.1"
                     /translation="MKKNFIGKSILSIAAISLTVSTFAGESHAQTKNAETVKKYNEYQ
                     TNFKKQVNKKVVDAQKAVNLFKRTRTVATHRKAQRAVNLIHFQHSYEKKKLQRQIDLV
                     LKYNTLK"
     gene            complement(1439..1606)
                     /locus_tag="NBH32_001733"
     CDS             complement(1439..1606)
                     /locus_tag="NBH32_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515326.1"
                     /translation="MKRIVLLRNVIVDGPIWLNHVDYLVKSVLLKSIKLRFQEVRKIE
                     NLVKSMNRIHI"
     gene            complement(1735..2238)
                     /locus_tag="NBH32_001734"
     CDS             complement(1735..2238)
                     /locus_tag="NBH32_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HCD1515327.1"
                     /translation="MSKWIIVSDNHTESGVLYQIYEMHPDADVYLHLGDSEFAYDDTE
                     LSLFNRVKGNCDFYPEFENEAVAKCNDVYAFYTHGHLYQVNRTRHLLAEQARELGCVF
                     AFYGHTHVAKYEYINGVHVINPGSISQSRSSMEETYAEVLIDDQTLHGTVNFKNRHHE
                     TISHTTF"
     gene            complement(2231..2818)
                     /locus_tag="NBH32_001735"
     CDS             complement(2231..2818)
                     /locus_tag="NBH32_001735"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCD1515328.1"
                     /translation="MKEIVIASNNQGKINDFKVIFPDYHVIGISELIPDFDVEETGST
                     FEENAILKSEAAAKALNKTVIADDSGLEVFALNGEPGIYSARYAGENKSDEANIEKLL
                     NKLGNTTDRRAQFVCVISMSGPDMETKVFKGTVSGEIADGKYGDNGFGYDPIFYVPKL
                     DKTMAQLSKEEKGQISHRRNAINLLEAYLAGDQHV"
     gene            complement(2830..3630)
                     /gene="racE"
                     /locus_tag="NBH32_001736"
     CDS             complement(2830..3630)
                     /gene="racE"
                     /locus_tag="NBH32_001736"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCD1515329.1"
                     /translation="MNKPIGVIDSGVGGLTVAKEIMRQLPNETIYYLGDIGRCPYGPR
                     PGEQVKQYTVEIARKLMEFDIKMLVIACNTATAVALEYLQKTLSIPVIGVIEPGARTA
                     IMTTRNQNVLVLGTEGTIKSEAYRTHIKRINPHVEVHGVACPGFVPLVEQMRYSDPTI
                     TSIVIHQTLKRWRNSESDTVILGCTHYPLLYKLIYDYFGGKKTVISSGLETAREVSAL
                     LTFSNEHASYTEHPDHRFFATGDPTHITNIIKEWLNLSVNVERISVND"
     gene            complement(3866..4681)
                     /gene="sdhB"
                     /locus_tag="NBH32_001737"
     CDS             complement(3866..4681)
                     /gene="sdhB"
                     /locus_tag="NBH32_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HCD1515330.1"
                     /translation="MTDQPVKDTPQHETQSKPKQKTVKLIIKRQDTSDSKPYEETFEI
                     PYRENLNVIACLMEIRRNPVNIKGEKTTPVVWDMNCLEEVCGACSMVINGRARQSCSA
                     IVDQLEQPIRLEPMNTFPVIRDLQVDRSRMFDNLKRMKAWIPIDGTYDLGPGPRMPEK
                     KRQTAYELSKCMTCGVCLEVCPNVTENNKFVGAQAISQVRLFNLHPTGSMTKDERLNA
                     LMGTGGLQQCGNSQNCVNACPKGIPLTTSIAAMNRETTFHMFKSFFGSDHEVE"
     gene            complement(4681..6447)
                     /gene="sdhA"
                     /locus_tag="NBH32_001738"
     CDS             complement(4681..6447)
                     /gene="sdhA"
                     /locus_tag="NBH32_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCD1515331.1"
                     /translation="MAEKHLIVVGGGLAGLMSTIKAAEKGAHVDLFSVVPVKRSHSVC
                     AQGGINGAVNTKGEGDSPWIHFDDTVYGGDFLANQPPVKAMTEAAPKIIHLLDRMGVM
                     FNRTNEGLLDFRRFGGTLHHRTAYAGATTGQQLLYALDEQVRAYEVDGLVTKYEGWEF
                     LGIVKGDDDSARGIVAQNMTTAEIETFGSDAVIMATGGPGIIFGKTTNSMINTGSAAS
                     IVYQQGAIYANGEFIQIHPTAIPGDDKLRLMSESARGEGGRIWTYKDGKPWYFLEEKY
                     PDYGNLVPRDIATREIFDVCINQKLGINGENMVYLDLSHKDPHELDVKLGGIIEIYEK
                     FTGDDPRKVPMKIFPAVHYSMGGLYVDYDQMTNIKGLFAAGECDFSQHGGNRLGANSL
                     LSAIYGGTVAGPNAIDYIANIDRSYTDMDESIFEKRKAEEQERFEKLMAMRGTENAYK
                     LHRELGEIMTANVTVVRENEKLLETDKKIVELMKRYENIDMEDTQTWSNQAVFFTRQL
                     WNMLVLARVITIGAYNRNESRGAHYKPEFPERNDEEWLKTTMASFQGAFEKPQFTYDD
                     VDVSLIPPRKRDYTSKSKGGKK"
     gene            complement(6499..7113)
                     /locus_tag="NBH32_001739"
     CDS             complement(6499..7113)
                     /locus_tag="NBH32_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b558 subunit"
                     /protein_id="HCD1515332.1"
                     /translation="MAQSKNEFYLRRIHSLLGIIPIGAFLVVHLLVNHQATQGAEAFN
                     KASNFMESLPFLIIVEFLFIYIPLLYHGLFGIHIAFTAKENVGHYSIFRNWMFFFQRV
                     SGILTFIFIGIHLWQTRLQKAFYGKEVNYDLMHETLQHPGWAIFYIICIIAVVFHFAN
                     GLWSFLVTWGGLQSPKSQRVFTWVSLIVFLVISYIGVTAIIAFM"
     gene            complement(7437..9218)
                     /gene="uvrC"
                     /locus_tag="NBH32_001740"
     CDS             complement(7437..9218)
                     /gene="uvrC"
                     /locus_tag="NBH32_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCD1515333.1"
                     /translation="MEDYKQRIKNKLNVVPMEPGCYLMKDRNDQVIYVGKAKKLRNRL
                     RSYFTGAHDAKTTRLVGEIRHFEFIVTSSETESLLLELNLIKQYQPRYNILLKDDKSY
                     PFIKITKEKYPRLLVTRTVKQGTGKYFGPYPNAYSAQETKKLLDRIYPYRKCDKMPDK
                     LCLYYHIGQCLGPCVYDVDLSKYAQMTKEITDFLNGEDKTILKSLEERMLTASESLDF
                     ERAKEYRDLIQHIQNLTNKQKIMSSDKTIRDVFGYSVDKGWMCIQVFFIRQGNMIKRD
                     TTMIPLQQTEEEEFYTFIGQFYSLNQHILPKEVHVPRNLDKEMIQSVVDTKIVQPARG
                     PKKDMVDLAAHNAKVSLNNKFELISRDESRTIKAIEELGTQMGIQTPIRIEAFDNSNI
                     QGVDPVSAMVTFVDGKPDKKNYRKYKIKTVKGPDDYKSMREVVRRRYSRVLNEGLPLP
                     DLIIVDGGKGHMNGVIDVLQNELGLDIPVAGLQKNDKHQTSELLYGASAEIVPLKKNS
                     QAFYLLHRIQDEVHRFAITFHRQTRQKTGLKSILDDIEGIGSKRKTLLLRSFGSIKKM
                     KEATLEDFKNIGIPENVAKNLHEQLHK"
     gene            9384..9479
                     /locus_tag="NBH32_001741"
     CDS             9384..9479
                     /locus_tag="NBH32_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515334.1"
                     /translation="MLDYHLTFIDLLIPPFSSRKQYDDLSFNFLY"
     gene            complement(9542..9856)
                     /gene="trxA"
                     /locus_tag="NBH32_001742"
     CDS             complement(9542..9856)
                     /gene="trxA"
                     /locus_tag="NBH32_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HCD1515335.1"
                     /translation="MAIVKVTDADFDSKVESGVQLVDFWATWCGPCKMIAPVLEELAA
                     DYEGKADILKLDVDENPSTAAKYEVMSIPTLIVFKDGQPVDKVVGFQPKENLAEVLDK
                     HL"
     gene            complement(10029..12377)
                     /locus_tag="NBH32_001743"
     CDS             complement(10029..12377)
                     /locus_tag="NBH32_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HCD1515336.1"
                     /translation="MRQKTLDVLEFEKIKSLVANETISDLGLEKVNQMMPATNFETVV
                     FQMEETDEIAQIYNKHRLPSLSGLSKVSAFIHRADIGGVLNVSELNLIKRLIQVQNQF
                     KTFYNQLVEEDEGVKYPILDDKMNQLPVLTDLFQQINETCDTYDLYDNASYELQGIRS
                     KISSTNQRIRQNLDRIVKSQANQKKLSDAIVTVRNERNVIPVKAEYRQDFNGIVHDQS
                     ASGQTLYIEPSSVVEMNNQISRLRHDEAIEKERILTQLTGYVAADKDALLVAEQVMGQ
                     LDFLIAKARYSRSIKGTKPIFKEECTVYLPKAYHPLLNRETVVANTIEFMEDIETVII
                     TGPNTGGKTVTLKTLGLIIVMAQSGLLIPTLDGSQLSIFKNVYCDIGDEQSIEQSLST
                     FSSHMTNIVEILKHADKHSLVLFDELGAGTDPSEGAALAMSILDHVRKIGSLVMATTH
                     YPELKAYSYNREGVMNASVEFDVDTLSPTYKLLMGVPGRSNAFDISKKLGLSLNIINK
                     AKTMIGTDEKEINEMIESLERNYKRVETQRLELDRLVKEAEQVHDDLSKQYQQFQNYE
                     KSLIEEAKEKANQKIKAATKEADDIIKDLRQLREQKGADVKEHELIDKKKRLDDHYEA
                     KSIKQNVQKQKYDKIVAGDEVKVLSYGQKGEVLEIVNDEEAIVQMGIIKMKLPIEDLE
                     KKQKEKVKPTKMVTRQNRQTIKTELDLRGYRYEDALIELDQYLDQAVLSNYEQVYIIH
                     GKGTGALQKGVQQHLKKHKSVSDFRGGMPSEGGFGVTVATLK"
     gene            complement(12387..14099)
                     /gene="polX"
                     /locus_tag="NBH32_001744"
     CDS             complement(12387..14099)
                     /gene="polX"
                     /locus_tag="NBH32_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HCD1515337.1"
                     /translation="MTKKDVIKLLEQIATYMELKGENTFKISAYRKAAQSLELDERPL
                     DEISDVTDLKGIGKGVAEVINDYRETGESQYLQQLQEEVPEGLIPLLKIQGLGSKKIA
                     KLYKELNIVDKASLQVACENGKVSELSGFAKKTEQNILEAVKQLGAKKDRYPIDQMRR
                     LNQEIIDYIDTLNYIDQYSSAGSFRRFKEMSKDLDFIISTDNPKAVQQQLLNIPNKVK
                     EVAVGNTKVSLELAYDDETIGVDFRLIEPSAFYHTLQHFTGSKEHNIRIRQLAKARDE
                     KVSEYGIEQADGTLIQYDSEAKIYEHFNVNFIPPAMREDGSEFDKDLSNIITLDDING
                     DIHMHTTYSDGAFSIRDMVEANIAKGYKFMVITDHSQSLHVANGLQVERLLRQNEEIK
                     ALDKEYSEIDIYSGTEMDILTDGSLDYDDEILAQLDYVIGAIHQSFNQSEEQIMERLA
                     NACRNPYVRHIAHPTGRIIGRRDGYKPNIEQLMALAEETNTVLEINANPHRLDLNADI
                     VRKYPNVKLTINTDAHHTNHLDFMNYGVATAQKGFVTKDRVINALSREAFKDFIENNI
                     KLKK"
     gene            complement(14172..14693)
                     /locus_tag="NBH32_001745"
     CDS             complement(14172..14693)
                     /locus_tag="NBH32_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HCD1515338.1"
                     /translation="MVIDFIIIIFFVYFVIVGFRRGFWLSMIHLSATIVSLWIASQFY
                     KSIVERLIVFIPYPKTTAFNTTFAFHFNHLQNRFEAIVAFLMITLFCKFILYLIIVTF
                     DKIIAYQNIHIFSRAMGMIVGVFMTIIVLHFTLYLLALYPNEALQHQLKISIVSHSLI
                     FHIPYLSAFTINL"
     gene            complement(14694..14960)
                     /gene="zapA"
                     /locus_tag="NBH32_001746"
     CDS             complement(14694..14960)
                     /gene="zapA"
                     /locus_tag="NBH32_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCD1515339.1"
                     /translation="MAQFKNKVNVSINDQLFTIVGEDNPEHIRYVAHLVDDKIKELGY
                     KAAGLDTSRKAILTAVNIMHEKVLLEEENRRLKQQIHKLQQREQ"
BASE COUNT         4628 a         2849 c         2096 g         5486 t
ORIGIN      
        1 tcaaattcat cattattctt tttcggttgc attaaacctt attaatttgc acttaatttt
       61 aattaatgaa gtgttaactg gtaaatgtat gaaaccgttt gtaaaatagc agtgtactcg
      121 cattagtaag tacaaatcta ttttacaggg agaacttatt atgaaaaaaa atattacaaa
      181 agtaattatt gcatctacag ttatcgctac aggtcttcta actcattcaa atgatgctaa
      241 agctttcttt agttatgaat ggaaaggttt agaaattgct aaaaaattag cagatgatga
      301 gaaaaaagat gaagaacgtg ctgatagatt aattaaagac gccgagaaag aaagaactta
      361 tacaggtaaa acggttgaag atttatatgt cattgctaaa aaattaggta aaggtaatac
      421 tatagctgta gtgagaatta tagatggtgg taaaaacggt tactatacgt ttgatatcac
      481 gcgtccatta gaagaacata gaaaaaatat tcccgtagtt aaaaacggcg aaatcgattc
      541 aattacttgg tattaaaaaa cacactgaat taaatagttg cacgccaaac gttagaaaaa
      601 aatgctaacg ttttggcgtg cttttttata tgctgcaact tccaatttat ttagcatctt
      661 tacgactgct taataaagca cgtatgatta acacggttgc cactatatcc gttacaattt
      721 ttatgattgt taatacattc gatttccttt tcacaatagc cacctcgctt gatcaaaaca
      781 taaacaacta ttgcattcac ttttaaataa ttatttttcc caataagctc accattcaaa
      841 tgaacacaat attataaact aattatacct ttgaatatag caaaaagcca cactctaaag
      901 catggcttct atcaattatt ttaaagtatt atattttaaa actagatcga tttgtctttg
      961 taattttttc ttttcatagc tgtgttggaa atgaattaaa ttaacagctc tttgtgcttt
     1021 acggtgtgtt gcaacagttc ttgtacgttt gaacaagttt acagcttttt gtgcatccac
     1081 aactttttta tttacttgtt ttttaaagtt tgtttgatac tcgttatatt tttttacagt
     1141 ttcagcgttt ttagtttgtg catgagattc accggcaaac gttgataccg ttaaactaat
     1201 agcagctatg cttaaaattg atttcccaat aaaattcttt ttcataatta ataacaccta
     1261 tcctcaaata tttattctgt atatatttat aataatagag atatacaaaa aactacatta
     1321 actatttatt aactaaacat taatatttgt gtcactacat ttctgaattt caagaatttt
     1381 gctactccat attttaacca ccaaattatt gatataattt atgcctttag aaatcctttt
     1441 aaatatgtat gcgattcatg cttttaacta aattttcaat tttcctgact tcttggaatc
     1501 ttaattttat actttttaac aatacacttt taaccaaata atcaacatga ttcaaccata
     1561 taggaccatc aacaatcaca ttcctcaaaa gtacaattct tttcataaca taaatgtgtt
     1621 attcattcca gttatcttat aaaattgtca gaaatacttt taaacaaata ttcatgtcac
     1681 gacctcctag ttaaatcagt ctataaacta aaaaaagtca tagctacttt ctatttaaaa
     1741 agtagtatga ctgattgttt cgtgatgtcg atttttgaaa ttgacagtgc catgtaaagt
     1801 ttgatcatca atcaagactt cagcatatgt ttcttccatt gaacttctag attgagatat
     1861 acttccagga ttaatcacat gaacaccatt aatatattca tattttgcca catgtgtatg
     1921 tccataaaaa gcaaatacac aacctaattc gcgtgcttgc tcagctaata aatgtcttgt
     1981 tcgattgact tgatataaat ggccgtgagt ataaaaagca tacacgtcgt tacactttgc
     2041 gaccgcttca ttttcaaatt ctgggtaaaa atcacaattg ccttttactc tattaaataa
     2101 gctaagttcc gtatcatcat acgcgaattc tgaatctcct aaatgtaaat atacatctgc
     2161 atctgggtgc atttcataaa tttgatataa aacgcctgat tcagtatggt tatcactcac
     2221 aataatccat ttagacatgt tgatcacccg caagataagc ttctaataaa ttaattgcat
     2281 ttcgtctatg gctaatttga cctttttctt ctttagaaag ttgcgccatg gttttatcta
     2341 atttcggcac ataaaatatc ggatcataac caaaaccatt atcgccatat tttccatctg
     2401 caatttcacc tgaaaccgta cctttaaata cttttgtttc catatcaggg ccactcatac
     2461 ttatgacaca aacaaattgc gcacgacgat cagttgtatt accaagctta tttaataatt
     2521 tttcaatatt tgcttcatcg cttttatttt caccagcata acgtgccgag tatatacctg
     2581 gctcaccatt taatgcaaaa acttcaagtc cactgtcatc agctatgacc gttttattca
     2641 atgcttttgc tgcagcttct gattttaata tagcattttc ttcaaatgtt gatcctgttt
     2701 cttccacatc aaaatctggt attagttctg aaatacctat tacgtggtaa tctggaaata
     2761 tcactttaaa gtcatttatt ttcccttgat tattcgatgc aataacaatc tctttcatta
     2821 aaaatcctcc tagtcattca ctgatatacg ttccacattg acagataaat ttaaccactc
     2881 tttgataatg ttagtaatgt gagtaggatc acctgttgca aaaaatcgat gatctggatg
     2941 ttcagtataa cttgcatgtt cattactaaa tgttagcaat gcactaactt cacgagccgt
     3001 ttctaatcca gacgaaatca ctgtcttttt accaccaaaa taatcataga taagtttata
     3061 gagcaatgga tagtgggtac atcctaaaat gacagtatca gactcactat tacgccaacg
     3121 tttcagtgtt tgatgaatga caatgcttgt aattgttgga tcactatatc tcatttgttc
     3181 tacaagtggc acaaaacctg gacaggcaac gccatgtacc tctacatgtg gattgatacg
     3241 tttaatatgc gtacgatatg cttcagattt aattgtgcct tccgttccta gtactaatac
     3301 attttgattt ctagtagtca ttattgctgt tctagcacct ggttcaatta cgccaatcac
     3361 tgggattgat aaggtctttt gtaaatattc taaagctaca gcagttgctg tattacaagc
     3421 aatcacgagc atttttatat caaattccat taatttacga gcgatttcaa ctgtatattg
     3481 ttttacttgt tctcctggtc ttggcccata tgggcatcgc ccaatatcac ctaagtaata
     3541 aatcgtctca tttggcaact gacgcataat ttctttagct actgtcaaac ctccgacacc
     3601 agagtctatt acacctattg gtttattcat attcggtcat ccttactttt aatttaattt
     3661 ttagtttatc ataactaagc attggatttt agtattatgc actgtgttta ccatttttgt
     3721 cattataata tttattttaa atcagcccac tatcatattg tcatgtaatc tgcttattaa
     3781 aaaaatccct tccaagttat tgtgtatctc cattcaattt aattttgaaa ggaacataac
     3841 attttaactc aaaagggatt aatttttatt ctacttcatg gtctgaacca aagaaagatt
     3901 taaacatgtg gaatgttgtt tctctgttca ttgctgcaat ggatgttgtt aatggaatac
     3961 ctttagggca agcattaaca cagttttgtg aattaccaca ctgctgtaag ccaccagtac
     4021 ccattaatgc atttaaacgt tcatctttag tcatagatcc tgttgggtgc aaattaaaca
     4081 aacgaacttg cgagattgct tgcgcaccaa cgaatttatt attttcagta acattaggac
     4141 aaacctctaa acatacacca catgtcatac atttagataa ttcataagct gtttgacgtt
     4201 ttttctctgg catacgtggt cccggaccta aatcatacgt tccatcaatt gggatccatg
     4261 ctttcatacg ttttaagtta tcgaacattc tagaacgatc aacttgtaag tcacggataa
     4321 ctgggaatgt attcataggc tctaatcgaa taggctgttc taattgatca acaatcgctg
     4381 aacatgattg tcttgcacga ccattgataa ccattgaaca cgcaccacat acttcttcta
     4441 aacagttcat gtcccatact actggcgttg ttttttcgcc tttaatatta actggattac
     4501 gtctaatttc cattaaacat gcaatgacat ttaaattttc acgatacgga atttcaaacg
     4561 tttcttcata tggttttgaa tcacttgtat cttgacgttt aataattaat tttactgttt
     4621 tttgtttcgg tttagattgt gtttcatgtt gtggtgtgtc ttttactggt tggtcagtca
     4681 ttatttttta ccccctttag acttacttgt gtaatcacgt ttacgaggtg gtattaaact
     4741 cacatcgacg tcatcataag taaactgtgg tttttcaaat gcaccttgga atgaggccat
     4801 tgttgttttt aaccactctt cgtcattacg ctctgggaat tctggtttat aatgggcacc
     4861 acgtgattca ttacgattat atgccccaat cgtaatcaca cgtgcaagta ctaacatatt
     4921 ccatagttga cgagtgaaga ataccgcttg gttactccaa gtttgtgtat cttccatatc
     4981 tatattttca taacgcttca tcaattcaac aatcttttta tctgtttcta acagtttttc
     5041 attttcacga acaactgtta cattagctgt cataatttct ccaagttcac gatgtaattt
     5101 ataagcattt tcagtaccac gcatagccat taatttttca aaacgttctt gttcttcagc
     5161 tttacgtttt tcaaaaatac tttcatccat atcagtatat gaacgatcaa tattagcaat
     5221 ataatcaatg gcattcgggc ctgcaactgt accaccgtaa atcgctgaca ataatgaatt
     5281 ggcacctaag cggttaccac catgttgaga gaagtcacat tctccagctg caaataaccc
     5341 tttaatattt gtcatttgat cataatctac atatagacca cccattgaat agtggacagc
     5401 tgggaaaatc ttcattggta ctttgcgtgg gtcatcacca gtaaattttt cataaatttc
     5461 aatgatacca cctagtttta catctaactc atgtggatct ttatgtgaca aatcaagata
     5521 taccatgttt tcgccattaa tacctaattt ttggttaata catacatcga aaatttcacg
     5581 cgttgcgata tcacgaggta ctaagttacc ataatcagga tatttctctt ctaagaagta
     5641 ccaaggctta ccatctttat atgtccaaat tcgtccacct tcaccacgtg ctgattcact
     5701 cattagtcga agtttatcat caccagggat tgcagtagga tgaatttgaa tgaactcacc
     5761 attagcataa atagcgcctt gttggtaaac aatggaagcc gctgatcctg tattaatcat
     5821 tgagtttgtt gttttaccga aaataatacc ggggccaccc gttgccataa taactgcatc
     5881 tgaaccaaat gtttcaatct cagcagttgt catattttgt gcaacgatac ctcttgcact
     5941 atcatcgtca cctttaacta tgccaaggaa ttcccatcct tcatacttcg taactaatcc
     6001 atctacttca tatgcacgaa cttgttcatc caatgcatat aataattgtt gtccagttgt
     6061 tgcccctgca tatgctgttc tgtggtgtaa tgtaccaccg aaacgtctaa aatctaatag
     6121 accttcattt gttctattga acattacgcc catacggtct aataaatgaa taattttagg
     6181 tgctgcctct gtcatcgctt taacaggtgg ttggtttgca aggaagtcgc caccatacac
     6241 tgtatcatca aagtgaatcc aaggagaatc gccttcccct ttagtattga ccgcaccatt
     6301 aatgccacct tgggcacaaa cagagtgcga acgctttact ggtacaactg agaacaaatc
     6361 tacatgtgca cctttttctg ccgctttaat tgttgacatt aagcccgcta ggccacctcc
     6421 gacaacaata agatgtttct ctgccataaa aatttcactc ccctaaattt tcaatctata
     6481 tttgttaaat gcgatgtatt acataaaggc aataattgca gtaacaccaa tatacgaaat
     6541 aactaagaat acgattaatg aaacccatgt aaatactcgt tgtgattttg gagattgaag
     6601 tcctccccaa gtaactaaga atgaccataa gccatttgca aagtggaaca caacagcaat
     6661 aatacaaata atataaaata ttgcccatcc aggatgttgc aatgtttcgt gcattaaatc
     6721 gtaattcact tctttgccgt aaaatgcttt ttgtaaacgt gtttgccata aatggatacc
     6781 aataaagata aatgttaaga taccactcac tctttggaag aagaacatcc agtttctaaa
     6841 aatcgagtaa tgtccaacat tttcttttgc tgtaaatgca atgtgtatac caaacaaacc
     6901 gtgatataac aacggaatgt atataaataa aaattctaca ataattagaa atggtaatga
     6961 ttccataaag ttagatgcct tattaaacgc ttcagcacct tgtgttgctt ggtgattcac
     7021 taataaatga acgaccaaaa atgcacctat tgggataata cctaataacg agtgaatacg
     7081 tcttagataa aattcatttt ttgattgagc caaaaggagt cccccctgtg aacgaatatt
     7141 taatttattg agctatttat attaaacgta cgcttaaccc cctaaagtga tatcgtttct
     7201 agcgttgtca ttatcattaa gcgaaacatt ttaaagacaa atacacactg tacgatcacc
     7261 aaactgcatg tcgaacaatg taacatttgg attcgatatt taaaattgct tgtgatgata
     7321 aactttctca tttagaaaac gcttccacgt acattcaaaa aaataacttt gttaaccata
     7381 ttgtaacatt atttcatata ttttggggca tgagaatgat tctcacgccc agtaatttat
     7441 ttatgcaatt gttcatgtag gttctttgcg acgttttcag gaatacctat atttttaaaa
     7501 tcttcaagtg tagcttcctt cattttcttg attgaaccga atgaacgtaa taataatgtt
     7561 ttacgtttgc taccgatacc ctctatatca tcaaggattg atttcaagcc tgtcttttga
     7621 cgtgtttgcc tatgaaatgt gattgcgaat ctgtgaacct catcttggat acggtgcaac
     7681 aaataaaatg cttggctatt tttcttcagt ggtactattt ctgcactagc gccatataat
     7741 aattcagatg tttggtgttt atcatttttc tgcaaacctg caacagggat atcaagacct
     7801 aattcgtttt gtagcacatc aataaccccg ttcatatgtc ctttaccacc atctactatt
     7861 attaaatcag gtaatggtaa tccttcgttt aaaacgcgag aatatcggcg tcttactact
     7921 tctctcattg atttgtaatc atctggacct ttaaccgttt tgattttata ctttctataa
     7981 tttttcttat ctggtttacc gtcgacaaat gtaaccattg ctgacactgg atccacacct
     8041 tgaatattag aattatcgaa tgcttcaatt ctaattggtg tttgaattcc catttgtgtt
     8101 ccaagttctt caatagcttt aattgttctg gactcatcac gtgatattaa ttcaaattta
     8161 ttatttaaag atactttagc gttatgtgca gctaggtcaa ccatatcttt tttgggacct
     8221 cgcgcaggtt gaacgatttt agtgtccaca acagattgaa tcatttcttt atccaaatta
     8281 cgtggtacat gaacttcctt aggtaaaata tgttggttta agctataaaa ttgtccaata
     8341 aatgtataaa attcttcttc ttctgtttgc tgtaatggaa tcatcgttgt atctcgcttt
     8401 atcatattac cttgtcgtat aaagaaaact tggatacaca tccatccttt atcaacgcta
     8461 taaccaaaga catcacgaat cgttttatct gatgacataa ttttttgttt gttagttaga
     8521 ttttgaatat gttgaattaa atctctatat tctttagccc gttcaaaatc aagtgattca
     8581 cttgcagtta acattcgctc ttctaaactt tttaaaattg ttttgtcttc cccattcaga
     8641 aaatcagtaa tttccttcgt catttgtgcg tatttactca aatcaacgtc atatacacat
     8701 ggtcctaaac attgtccaat atggtaataa agacataatt tatctggcat cttatcacat
     8761 ttgcgatatg gatatattct gtctaataac tttttagttt cttgagcaga atatgcattc
     8821 ggatacggtc cgaaatattt gccagtacct tgttttacag ttctcgtcac tagtagtcta
     8881 ggatattttt ccttcgtaat tttaatgaat ggataacttt tatcatcctt taataatata
     8941 ttatatcttg gttgatattg tttaatcaga ttcaattcca gtaaaagtga ctctgtttca
     9001 cttgacgtga caataaattc aaaatgccgt atttctccaa ccagtctcgt tgttttagca
     9061 tcatgagcac ccgtaaaata tgatctcaat cgatttctta gctttttagc tttgccaaca
     9121 tatatcactt gatcattacg atctttcatt aaatagcagc ctggttccat aggtacgaca
     9181 tttaatttat ttttaattcg ttgcttatag tcttccaata atatcccccc ttctaatttt
     9241 aaaatgaatg atgctacaaa agtgcttcaa atttatcacc actaattcta gctcataatc
     9301 aacatcttcg agtatgactt ttcatgcaca tcatgtaaaa ttgaacattg ttttatcaaa
     9361 ccaactgcta caaatctatc agcatgctag attatcattt aacgttcata gatttattaa
     9421 tcccaccatt ctcaagtcgt aaacaatatg atgacttatc attcaacttt ttatattaaa
     9481 aacacaaaca aaaactggag caattcatta aagaaatgcc ccagtcgtca ttggttgtaa
     9541 cttataaatg tttatctaaa acttcagcta agttttcttt tggttggaaa ccaacaactt
     9601 tatcaactgg ttgaccgtct ttaaagacga ttaatgttgg aatactcatc acttcatatt
     9661 tagctgcagt agatggattt tcatcaacat ctaattttaa aatgtcagct ttaccttcat
     9721 agtcagctgc taattcttct aataccggag cgatcatttt acatggacca caccatgttg
     9781 cccaaaaatc tactaattgt acaccagatt ctacttttga atcgaaatct gcatctgtta
     9841 cttttacgat tgccataaat gccaatcctc cttaattatt attatgatgt tcaaattata
     9901 tcagataaca tgcttcatgt atttctattt gctcaatttt tatgcttatt tcaaataaca
     9961 tgtttgaaat gtattcttgt tgctttacat tattttaact gcagctattt aatttatcaa
    10021 attataattt attttagtgt tgcaacggta acgccaaatc caccttcgct tggcatacca
    10081 cctctaaagt cactaacact tttatgcttt ttcaaatgtt gttgtacacc tttttgaagt
    10141 gcacctgtac ctttaccgtg aatgatataa acttgttcgt aattacttaa aactgcttga
    10201 tctaaatatt gatctagttc aattaaagca tcctcataac gatagcctcg taagtctagt
    10261 tcagttttaa ttgtttgacg gttttgacgt gtaaccattt tcgttggctt aactttttct
    10321 ttttgttttt tctctaaatc ttcaataggt aacttcattt taataattcc catttgaaca
    10381 attgcttctt catcattgac aatttctaaa acttcaccct tttgaccgta agataatact
    10441 tttacttcat caccagcaac aattttatcg tatttttgct tttgtacatt ttgctttata
    10501 gatttcgctt cataatgatc atctaatcgt ttcttcttat caatcaattc atgttcttta
    10561 acatctgcac ctttttgttc acgcaattgt cttaagtctt taataatatc gtcagcttct
    10621 tttgttgcag ctttaatctt ctgatttgct ttttctttcg cttcctctat tagagacttt
    10681 tcataatttt ggaattgttg atactgctta gataaatcat catgcacttg ctccgcttct
    10741 tttacaagac ggtccagttc taacctctgt gtctctacac gtttgtaatt acgctctaat
    10801 gattcaatca tttcatttat ttctttttca tcagtaccaa tcatcgtctt agccttatta
    10861 ataatattca aactaagacc taacttttta gaaatgtcaa aagcatttga acgacccggc
    10921 acacccatta ataacttata cgttggactc aaagtatcta catcaaattc tacactcgca
    10981 ttcataacgc cttctcgatt ataactatat gctttaagtt caggatagtg cgtcgttgcc
    11041 attactagag aaccaatttt tctaacatga tctaaaatgc tcattgctaa tgcagcacct
    11101 tcacttggat ctgtacctgc acctaattca tcaaataaaa ctaaactatg tttgtctgca
    11161 tgctttaaaa tttcaactat attcgtcata tgagatgaaa aagttgataa tgattgttct
    11221 attgattgtt catctccgat atcgcaatat acatttttaa atatacttaa ctgactacca
    11281 tcaagtgttg gaatcaacaa tcctgattga gccataacaa taattaaacc taatgttttt
    11341 aatgttactg ttttaccacc tgtattcggt cctgtaataa ttaccgtttc aatatcttcc
    11401 ataaattcga tggtattagc tacaacagtc tcacgattta ataatggatg gtatgcttta
    11461 ggtaaatata cagtacattc ctctttaaat atcggctttg ttcctttaat acttctacta
    11521 tatctcgctt ttgcgattaa aaaatctaac tgacccatga cttgttctgc cacaagtagt
    11581 gcatctttgt ccgcagccac ataaccagtt agttgcgtta aaatgcgttc tttttcaatt
    11641 gcttcatcat gacgtaatcg actaatttga ttattcattt caacaactga tgatggctca
    11701 atatacaatg tttgtcctga agcagattga tcatgtacaa tcccattaaa atcttgtcga
    11761 tattcagctt tgacaggtat aacgtttctt tcattcctaa ctgttacaat tgcatctgat
    11821 aattttttct gatttgcttg gcttttaaca atacggtcca aattttgtct aatacgttga
    11881 ttcgtgctag aaattttact tctaatccct tgcaattcat aactcgcatt atcatataaa
    11941 tcatacgtat cgcatgtttc atttatttgt tgaaaaagat cggttaacac aggtaattga
    12001 ttcatcttgt catctaatat tgggtattta acaccttcat cttcttcaac caattgatta
    12061 taaaatgtct taaattgatt ttgtacttga attaatcttt ttatcaagtt aagctctgat
    12121 acatttaaaa cgccgccaat atcagcgcga tgaatgaatg ctgatacttt agataagcca
    12181 ctcaagcttg gtaaacgatg cttattatag atttgagcaa tctcatccgt ttcttccatt
    12241 tgaaaaacaa ccgtttcaaa attagtagct ggcatcattt gattgacctt ttccaagcct
    12301 aagtcactaa ttgtttcatt ggcaacgagt gattttattt tttcaaattc taagacgtct
    12361 aatgtttttt gtctcataaa atccctctat ttcttaagtt ttatattatt ttcaataaag
    12421 tctttaaaag cttcacgcga taatgcgtta atcactctat cttttgttac aaatcctttt
    12481 tgcgcagttg ctacgccata attcataaaa tctaaatgat ttgtatgatg cgcatcagtg
    12541 ttaatagtta atttcacatt tggatattta cgaacgatat cagcgttcag atccaatcga
    12601 tgtggattgg cattaatttc taatactgta ttcgtttctt cagctaatgc cattaattgt
    12661 tcaatattcg gtttataacc atctcttcta cctataatac gccctgttgg atgcgctata
    12721 tgtcgcacgt atggattgcg acatgcatta gctaatcgtt ccataatttg ttcttctgat
    12781 tggttaaagc tttgatgaat agctccaatt acataatcaa gttgtgctaa aatttcatca
    12841 tcataatcca gcgagccatc agttaatata tccatttctg tacctgaata aatatcaatt
    12901 tcactatatt ctttatctaa agccttaatt tcttcgtttt gtcttaaaag tctttctact
    12961 tgtaagccat tagcaacatg taaactttgt gaatgatcag taattaccat gaatttataa
    13021 ccttttgcga tatttgcttc taccatgtct cgaatagaaa acgcaccatc actatacgtt
    13081 gtatgcatat gaatatcacc attaatatca tctaatgtaa tgatattact tagatcttta
    13141 tcaaattcgc taccatcttc tcgcatagca ggtggtataa aattcacatt aaaatgttca
    13201 tatatcttgg cttcactatc atattgaatt aatgtaccat cagcttgttc aattccatat
    13261 tcacttactt tttcatcacg tgctttagca agttgtcgaa ttcttatatt atgttctttt
    13321 gacccagtaa aatgctgcaa tgtatgataa aaagcacttg gttcaattaa tcgaaaatcg
    13381 acaccaatcg tttcatcatc atacgctaat tctaatgaaa cttttgtgtt ccccactgca
    13441 acttctttta ctttattggg aatatttaat aattgctgct gcactgcttt tgggttatcg
    13501 gtacttatta tgaaatctaa atctttgctc atttctttaa aacgacggaa gcttcctgca
    13561 gatgaatatt gatcgatata atttaatgta tctatataat caatgatttc ttgattaagt
    13621 cttctcattt gatcaattgg atatctatct ttcttagcac caagttgttt cacagcttct
    13681 aatatgtttt gttccgtttt cttagcaaat ccgcttaatt cactaacttt tccattttca
    13741 caagcaactt gaagtgacgc tttatcaaca atattcaact ctttatatag cttagcaatt
    13801 ttcttgcttc caagtccttg aattttcaaa agtggaataa gaccttccgg aacttcttcc
    13861 tgtaattgct gtaaatactg agattcaccg gtctcacggt aatcattgat tacttctgca
    13921 acacctttac caatgccttt taagtccgtt acatcagata tttcatctaa tggtcgttca
    13981 tctaattcaa gactttgagc tgcttttcga tacgctgata ttttaaaagt attttcccct
    14041 tttaattcca tataagtagc aatttgttct aatagtttga taacatcttt ttttgtcata
    14101 ataacactcc ataaaaagaa gaccaggacg tatcattaat atataccttt gtcctgacct
    14161 cttatgataa tttataaatt aatggtgaaa gccgataaat atgggatgtg aaaaatcaat
    14221 gaatgactca caatagatat tttaagctga tgttgtaatg cttcgttagg atataatgcc
    14281 aatagatata acgtaaagtg taagacaatt atcgtcataa acacaccaac tatcattccc
    14341 attgcacgac tgaaaatatg aatgttttga tacgctatta ttttatcaaa agttacgata
    14401 attagataca aaatgaactt acaaaacaat gtaatcatta aaaaagctac aatcgcttca
    14461 aatcgatttt gtagatgatt aaaatgaaac gcaaaagttg tattaaatgc tgttgtttta
    14521 ggatatggaa taaatacaat taatctttct acaatagatt tgtaaaattg actggcaatc
    14581 cacaatgata caatcgttgc actcaaatgt atcatagata accaaaaacc tcgtctgaat
    14641 ccaacgatga caaaatacac aaagaaaatg attatgataa aatcaatgac catttattgc
    14701 tcacgctgct gcaatttgtg aatttgttgt ttcaaacgtc gattttcttc ttctagtagt
    14761 actttttcat gcataatatt cacagcagtt aatattgctt ttcttgaagt atctaaacct
    14821 gctgctttat accctaattc ttttatttta tcatcaacta aatgtgctac atatcgtatg
    14881 tgctctgggt tatcttcccc aacaattgta aaaagctgat cattaattga tacatttacc
    14941 ttgtttttaa actgtgccat ttataatttc tcctgatcct ttttttaaaa tctaaattca
    15001 cgttataaaa tatgactgga tagtttgtct gaatttgata ctaatattgt tatattgca
//
LOCUS       DAHHOJ010000069        14894 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.069, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000069 DAHHOJ010000000
VERSION     DAHHOJ010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14894)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14894)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14894
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.069"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            258..1076
                     /locus_tag="NBH32_001747"
     CDS             258..1076
                     /locus_tag="NBH32_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HCD1515340.1"
                     /translation="MNNSEQLIALKESETAFLKYFNKADYELVDFSVVEKLDWKQLNH
                     EDLQQMGERNFWQHEHQIYALRNDFTDQLLRYYSMYPTAATKVAYTGLIIRNNEAAVQ
                     VGLENYAPSLANVQQSLKLFIQFIQQQLRDNVHFVVLGHYQLLDALLDKSLQTPDILS
                     MIEERNLSGLVTYLSTEHPIVQILKENTQQQLNVLEHYIPNDHPALVELKIWERWLHT
                     QGYKDIHLDITAQPPRSYYTGLFIQCHFAENESRVLTGGYYKGSIEGFGLGLTL"
     gene            1094..1708
                     /locus_tag="NBH32_001748"
     CDS             1094..1708
                     /locus_tag="NBH32_001748"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCD1515341.1"
                     /translation="MLRIAIAKGRLMDSLINYLDAIEYTTLSETLKNRERQLLLSVDN
                     IECILVKGSDVPIYVEQGMADIGIVGSDILDERQYNVNNLLNMPFGACHFAVAAKPET
                     TNYRKIATSYVHTAETYFKSKGIDVELIKLNGSVELACVVDMVDGIVDIVQTGTTLKA
                     NGLVEKQHISDINARLITNKAAYFKKSQLIEQFIRSLEVSIANA"
     gene            1701..2951
                     /locus_tag="NBH32_001749"
     CDS             1701..2951
                     /locus_tag="NBH32_001749"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCD1515342.1"
                     /translation="MLNAQQFLNQFSLEAPLDESLYPIIRDICQEVKVHGDKALKMYN
                     LTFDHTKTDHLEISHEQIKAAFDTLDEKTKQALQQSYERIKAYQESIKQTNQQLEESV
                     ECYEIYHPLESVGIYVPGGKASYPSTVLMTATLAQVAGVENIVVVTPPQPNGVSQEVL
                     AACYITQVNQVFQVGGAQSIAALTYGTETIPKVDKIVGPGNQFVAYAKKYLFGQVGID
                     QIAGPTEIALIIDDTTDLDAIVYDVFAQAEHDELARTYVISEDAQVLKDLESRIAKAL
                     PNVDRYDIVSKSIANQHYLIHASNFDEACHVMNTIAPEHASIQTVNPQPYIEKVKYVG
                     ALFIGHYSPEVIGDYVAGPSHVLPTNRTARFTNGLSVNDFLTRNTVIHLSKDTFEQIA
                     DSAQHIAHVEALYNHQQSILIRQS"
     gene            2967..3980
                     /locus_tag="NBH32_001750"
     CDS             2967..3980
                     /locus_tag="NBH32_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate aminotransferase family
                     protein"
                     /protein_id="HCD1515343.1"
                     /translation="MIYIDKNESPVTPLDEKTMTSIISATPYNLYPDAAYEQFKEAYA
                     KFYGLSPEQIIAGNGSDELIQKLMLIMPEGPALTLNPDFFMYQAYAAQVNREIAFVDA
                     GSDLTFDLETILTKIDEVQPSFFIMSNPHNPSGKQFDTAFLTAIADKMKALNGYFVID
                     EAYLDYGTAYDVELAPHILRMHTLSKAFGIAGLRLGVLISTAGTIKHIQKIEHPYPLN
                     VFTLNIATYIFRHREETSRFLTMQRQLAEQLKQIFDTHVADKISVFPSNANFVLTKGS
                     AAQQLGQYVYEQGFKPRFYDEPVMKGYVRYSIATEPQLNQLEEIVKEWSAKYDLSKTT
                     KHS"
     gene            3949..4527
                     /gene="hisB"
                     /locus_tag="NBH32_001751"
     CDS             3949..4527
                     /gene="hisB"
                     /locus_tag="NBH32_001751"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HCD1515344.1"
                     /translation="MIYQKQRNTAETQLNISISDDQSPSHINTGVGFLNHMLTLFTFH
                     SGLSLNIEAQGDIDVDDHHVTEDIGIVIGQLLLEMIKDKKHFVRYGAMYIPMDETLAR
                     VVVDISGRPYLSFNASLSKEKVGTFDTELVEEFFRAVVINARLTTHIDLIRGGNTHHE
                     IEAIFKAFSRSLGIALTATDDQRVPSSKGVIE"
     gene            4524..5102
                     /gene="hisH"
                     /locus_tag="NBH32_001752"
     CDS             4524..5102
                     /gene="hisH"
                     /locus_tag="NBH32_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCD1515345.1"
                     /translation="MIVIVDYGLGNISNVKRAIEHLGYEVVVSNNQNIIDQAETIILP
                     GVGHFKDAMSEIKRLNLDAILAKNTDKKMIGICLGMQLMYEYSDEGDASGLGFIPGNI
                     SRIQTEYPVPHLGWNNLVSKHPMLNQDVYFVHSYQAPMSENVIAYAQYGTDIPAIVQF
                     NNYIGIQFHPEKSGTYGLQILRQAIQGGFIND"
     gene            5095..5799
                     /locus_tag="NBH32_001753"
     CDS             5095..5799
                     /locus_tag="NBH32_001753"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-((5-
                     phosphoribosylamino)methylideneamino)imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCD1515346.1"
                     /translation="MIELWPAIDLIGSTSVRLTEGKYDSEEKMSRSAEESIAYYSRFE
                     CVNRIHIVDLIGAKAQHAREFDYIKSLRRLTTKDIEVGGGIRTKSQIMDYFAAGINYC
                     IVGTKGIQDTDWLKEMAHTFPGRIYLSVDAYGEDIKVNGWEEDTELNLFSFVRRLSDI
                     PLGGIIYTDIAKDGKMSGPNFELTGQLVKATTIPVIASGGIRHQQDIQRLASLNVHAA
                     IIGKAAHQASFWEGLK"
     gene            5796..6554
                     /gene="hisF"
                     /locus_tag="NBH32_001754"
     CDS             5796..6554
                     /gene="hisF"
                     /locus_tag="NBH32_001754"
                     /EC_number="4.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCD1515347.1"
                     /translation="MIKKRIIPCLDVKDGRVVKGIQFKGLRDIGNPVDLAMYYNEAGA
                     DELVFLDISKTEEGHSLMLEVIEQTASRLFIPLTVGGGIQSLDDITQLLNHGADKVSL
                     NSSALKNPQLIKQASDKFGRQCICIAIDSYYDPERKAHYCCTHGGKKMTNIKVYDWVQ
                     QVEQLGAGELLVTSMGHDGMKQGFDIEHLAKIKSLVNIPIIASGGGGNAQHFVELFDQ
                     TDVSAGLAASILHDRETTVQSIKEVIRQGGIAVR"
     gene            6551..7183
                     /locus_tag="NBH32_001755"
     CDS             6551..7183
                     /locus_tag="NBH32_001755"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCD1515348.1"
                     /translation="MTNYKIDFSKGLVPAILQDNQTKQVLMLGYMNQEAFDKTIEDGV
                     VCFYSRSKQRLWTKGETSGHTQLVKDIHVDCDNDTILIDVIPNGPTCHTGSQSCFNTE
                     VPFSVQTLAQTVQDSAQSNNEKSYTKYLLTEGIEKITKKYGEEAFEVVIEAIKGDKKA
                     FVSEVADELYHLFVLMHALGVDFSEIEAELARRHHKRNNFKGERQNIEQW"
     gene            8269..10311
                     /gene="lip1"
                     /locus_tag="NBH32_001756"
     CDS             8269..10311
                     /gene="lip1"
                     /locus_tag="NBH32_001756"
                     /EC_number="3.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK domain-containing triacylglycerol lipase
                     Lip1"
                     /protein_id="HCD1515349.1"
                     /translation="MKSQNKYSIRKFSVGASSILIATLLFLSGGQAQAAEKQVNMGNS
                     QEDTVTAQSIGDQQTRENANYQRENGVDEQQHTENLTKNLHNDKTISEENHRKTDDLN
                     KDQLKDDKKSSRNNKNIQRDTTKNNNANPSDVNQGLEQAINDGKQSKVASQQQSKEAD
                     NSQDSNANNNLPSQSRTKEAPSLNKLDQTSQREIVNETEIEKVQPQQNNQANDKITNY
                     NFNNEQEVKPQKDEKTLSVSDLKNNQKSPVEPTKDNDKKNGLNLLKSSAVATLPNKGT
                     KELTAKAKDDQTNKVAKQGQYKNQDPIVLVHGFNGFTDDINPSVLAHYWGGNKMNIRQ
                     DLEENGYKAYEASISAFGSNYDRAVELYYYIKGGRVDYGAAHAAKYGHERYGKTYEGI
                     YKDWKPGQKVHLVGHSMGGQTIRQLEELLRNGSREEIEYQKKHGGEISPLFKGNNDNM
                     ISSITTLGTPHNGTHASDLAGNEALVRQIVFDIGKMFGNKNSRVDFGLAQWGLKQKPN
                     ESYIDYVKRVKQSNLWKSKDNGFYDLTREGATDLNRKTSLNPNIVYKTYTGEATHKAL
                     NSDRQKADLNMFFPFVITGNLIGKATEKEWRENDGLVSVISSQHPFNQAYTNATDKIQ
                     KGIWQVTPTKHDWDHVDFVGQDSSDTVRTREELQDFWHHLADDLVKTEKVTDTKQA"
     gene            complement(10702..11754)
                     /gene="icaC"
                     /locus_tag="NBH32_001757"
     CDS             complement(10702..11754)
                     /gene="icaC"
                     /locus_tag="NBH32_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide intercellular adhesin
                     biosynthesis/export protein IcaC"
                     /protein_id="HCD1515350.1"
                     /translation="MKKIRLELVYLRAIICAIIIVTHLLTQITLKHENMEGGSLVLQF
                     YIRNIVIFGTPCFIILSQLLTTLNYQKVTYRYLTTRVKYILIPYILMGLFYSYSESLL
                     TDSSFNKQFIENVLLGQWYGYFIVVIMQFFILSYIIFKINYNLFNSKILLLLSFILQQ
                     SFLYYFTNNTAFHDTVLHYYPLSENTIIFGWIFYFFLGAYMGYNYERVLNFLERYLVI
                     MIVLAVATYFVFIALANGDYWNVTSFSYSLTPYNSIMFIVILGICTHFKTMLFNTIQM
                     ISAFSFFIYLLHPIILDSLFAYTNIFEDNTMVFLAISLLFILGLCIGVGMILREFYIF
                     RFIIGKQPYKLNINAY"
     gene            complement(11741..12613)
                     /gene="icaB"
                     /locus_tag="NBH32_001758"
     CDS             complement(11741..12613)
                     /gene="icaB"
                     /locus_tag="NBH32_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intercellular adhesin biosynthesis
                     polysaccharide N-deacetylase"
                     /protein_id="HCD1515351.1"
                     /translation="MKYRKLIILVLSILIILPVSTLDGHHIANADDDSPKKLKYKENS
                     ALALNYHRVRKANFLNNFIYFFSSSKEIKNYSVSQSQFESQIKWLKSHDAKFLTLKEF
                     LYYKKKGKFPKRSVWINFDDMDETIYENAYPILKKYKIPATGFIITGHVGEENFHNLD
                     MISKKELKEMYKTGLWEFETHTHDLHNLSKNNKSKLMKSSEATIIKDLNKSEKYLTKN
                     FKKSQKTIAYPYGLMNNDKLPVIKKAGLKYGFSLEEKAVTPNSNDYYIPRILISDDAF
                     EHLIKRWDGFHEKD"
     gene            complement(12610..12915)
                     /gene="icaD"
                     /locus_tag="NBH32_001759"
     CDS             complement(12610..12915)
                     /gene="icaD"
                     /locus_tag="NBH32_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular adhesion protein D"
                     /protein_id="HCD1515352.1"
                     /translation="MVKPRQREYPTLKSSLNIVRETALIAISCVFWIYCLVVLLVYIG
                     TIFEIHDESINTIRVALNIENTEILDIFETMGIFAIIIFVFFTISILIQKWQRGRES"
     gene            complement(12879..14117)
                     /gene="pgaC"
                     /locus_tag="NBH32_001760"
     CDS             complement(12879..14117)
                     /gene="pgaC"
                     /locus_tag="NBH32_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly-beta-1,6 N-acetyl-D-glucosamine synthase"
                     /protein_id="HCD1515353.1"
                     /translation="MQFFNFLLFYPVFMSIYWIVGSIYFYFTREIRYSLNKKPDINVD
                     ELEGITFLLACYNESETIEDTLSNVLALKYEKKEIIIINDGSSDNTAELIYKIKENND
                     FIFVDLQENRGKANALNQGIKQASYDYVMCLDADTIVDQDAPYYMIENFKHDPKLGAV
                     TGNPRIRNKSSILGKIQTIEYASLIGCIKRSQTLAGAVNTISGVFTLFKKSAVVDVGY
                     WDTDMITEDIAVSWKLHLRGYRIKYEPLAMCWMLVPETLGGLWKQRVRWAQGGHEVLL
                     RDFFSTMKTKRFPLYILMFEQIISILWVYIVLLYLGYLFITANFLDYTFMTYSFSIFL
                     LSSFTMTFINVIQFTVALFIDSRYEKKNMAGLIFVSWYPTVYWIINAAVVLVAFPKAL
                     KRKKGGYATWSSPDRGNTQR"
     gene            14282..14842
                     /gene="icaR"
                     /locus_tag="NBH32_001761"
     CDS             14282..14842
                     /gene="icaR"
                     /locus_tag="NBH32_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ica operon transcriptional regulator IcaR"
                     /protein_id="HCD1515354.1"
                     /translation="MKDKIIDNAITLFSEKGYDGTTLDDIAKSVNIKKASLYYHFDSK
                     KSIYEQSVKCCFDYLNNIIMMNQNKSNYSIDALYQFLFEFIFDIEERYIRMYVQLSNT
                     PEEFSGNIYGQIQDLNQSLSKEIAKFYDESKIKMTKEDFQNLILLFLESWYLKASFSQ
                     KFGAVEESKSQFKDEVYSLLNIFLKK"
BASE COUNT         5421 a         2230 c         2704 g         4539 t
ORIGIN      
        1 gcaaggacaa tatgtgcaag aagtacaact tcaatataaa aatcttggtt aagcattttt
       61 ataggcacaa atgtcttgcc tttttctttt gtaaaaattt aaaaacaata caccgaattt
      121 tctgaaaaag gttgtaatgt attgctaaaa atgataagtt attaatcata ttacacttta
      181 gtgtggtata gaagtaaagc gaacaacgaa aataccaagt gatattaaat acagtattga
      241 aagtaaaggg tggtcgagtg aataattcag aacaattaat tgcgttaaaa gaatcggaaa
      301 cagcattttt aaaatatttt aataaagcgg attatgagtt agttgatttt agtgtggttg
      361 aaaagttgga ctggaaacaa ttgaaccatg aggatttgca gcagatgggt gagcgaaatt
      421 tttggcaaca tgagcatcaa atttatgcac ttcgaaatga ttttacagat caattattgc
      481 gttattactc aatgtatcca actgcagcaa caaaagtggc atacacaggt ttaatcattc
      541 ggaataatga agcggctgta caagtgggtt tggaaaacta tgcaccatca ttagcgaatg
      601 tacaacagag cttaaagcta tttattcagt ttattcaaca gcagttgaga gacaacgtgc
      661 attttgttgt attgggacat tatcaattgc ttgatgcctt gctcgataaa tcgttacaaa
      721 caccagacat actatcaatg attgaggaac gtaatttatc gggattggtt acatatttgt
      781 cgactgaaca tccaatcgtg caaatcttga aagagaatac gcaacaacaa ttaaatgtat
      841 tggaacacta tattccaaat gaccatccag cactcgttga attaaaaata tgggaacgtt
      901 ggttacatac acaaggttac aaagacatcc atttagatat tactgcgcag ccacctagat
      961 cctattacac aggtttattt attcaatgtc attttgctga aaatgaatct cgagttttaa
     1021 caggtggtta ttacaaagga agcatcgaag ggtttggatt aggattaaca ctttaagtaa
     1081 gggagtatgc acaatgttaa gaatcgccat agcaaaaggg cgtctaatgg atagtttaat
     1141 taactattta gatgcaattg aatatacgac attatcagaa acattaaaaa atagagaacg
     1201 ccaattatta ttaagtgtag ataatattga atgcatttta gtaaaaggaa gtgacgttcc
     1261 aatctatgtg gaacaaggaa tggccgacat aggcattgtt ggtagcgaca tattagatga
     1321 gcgccaatat aatgttaata atttgttgaa tatgcctttt ggagcatgtc attttgcggt
     1381 tgcagcgaaa cctgaaacga ccaattatcg taaaatcgca acgagttatg tacatactgc
     1441 tgaaacatat tttaaatcaa aaggtattga tgtcgaattg attaaattga atggttctgt
     1501 tgaattagct tgtgtcgtag atatggtaga cggaattgtc gacatcgttc aaacaggtac
     1561 tacgctaaaa gcgaacggac tggttgaaaa gcaacatatt agtgatatca atgcaagatt
     1621 aataactaat aaagcagctt attttaaaaa atcacaatta atagagcaat ttattcgctc
     1681 tttggaggtg tctattgcca atgcttaatg cacaacaatt tttaaatcaa ttttcattag
     1741 aagcaccatt agatgagtca ttgtatccaa ttattcgcga tatttgtcag gaagttaaag
     1801 ttcatggaga taaggctttg aaaatgtata atctaacatt cgatcatacg aaaacagatc
     1861 atttagaaat tagtcatgaa caaattaaag cagcatttga cacattagat gaaaaaacaa
     1921 aacaagcatt acaacaaagt tatgaaagaa ttaaagcata tcaagaaagt attaaacaga
     1981 cgaatcaaca gttagaagaa tcagtggagt gttatgaaat ataccatcca ctagaaagtg
     2041 tgggtattta tgtgcctggt ggcaaagcaa gttatccatc aacggttcta atgacagcga
     2101 ctttagcaca agtagcaggt gtagaaaata ttgttgttgt gacaccacct caacctaacg
     2161 gagtatccca agaggtatta gctgcatgtt atattacgca agttaatcaa gtgtttcaag
     2221 ttggtggtgc tcaaagtatt gctgcattga cttatggaac agaaacgata cctaaagttg
     2281 ataagattgt aggtccaggt aaccaatttg ttgcatatgc caaaaaatat ttatttggac
     2341 aggtaggtat tgatcaaatt gcaggaccaa cagaaatagc actgattatt gacgacacca
     2401 cagatttaga tgccatcgta tatgatgtat ttgcgcaagc agaacatgat gaattagcac
     2461 gtacatatgt cattagtgaa gatgcgcaag tccttaaaga tttagaatca cgtattgcta
     2521 aagcattgcc taatgtggac agatacgaca ttgtttctaa aagtatcgct aatcaacact
     2581 accttatcca tgctagtaat tttgatgaag catgccatgt catgaataca atcgcgcctg
     2641 aacatgcgtc gattcaaaca gtaaatcctc aaccatatat tgaaaaagtg aaatatgtgg
     2701 gtgcattgtt tattggacat tattcgccag aggtcatagg agattacgtt gcaggtccaa
     2761 gtcatgtatt acctacaaat agaacagcta gatttaccaa tgggttatcg gtcaatgatt
     2821 tcttaacacg gaacacggtc atccatttat caaaagatac gtttgaacaa attgctgatt
     2881 cagcacaaca tattgctcat gttgaagcat tatacaatca ccagcagtct attttaatac
     2941 gtcagtctta ggggagtgta attgaaatga tttatattga taaaaatgaa agtccagtta
     3001 cgccgttgga tgaaaaaaca atgacgtcta ttattagtgc aacgccatat aatttatatc
     3061 ctgatgcagc atatgaacaa ttcaaggaag cttatgctaa gttttacgga ttatcgcctg
     3121 aacaaattat tgcaggaaat ggatctgatg aattgattca aaagttaatg ctgatcatgc
     3181 cagaaggtcc ggctttaacg ctaaatcctg atttttttat gtatcaagca tatgcggcac
     3241 aagtaaatcg tgaaattgca tttgtagatg caggatcaga tttaacgttt gatttggaaa
     3301 ccattttaac gaaaatcgat gaagtacaac catcattttt tattatgagt aatccacata
     3361 acccttcagg caagcaattt gatacggcat ttttaacagc tattgcagat aagatgaaag
     3421 cattaaacgg atactttgtc attgatgaag catatttaga ttatggtacg gcatatgacg
     3481 tggaactggc accacacatc ttaagaatgc atacattatc aaaggcgttt ggaattgccg
     3541 gcttaagatt aggtgtctta attagtactg ctggaacgat aaagcatatt caaaaaatag
     3601 aacatccata tccattaaat gtatttacgc taaatattgc gacttatatt tttagacata
     3661 gagaagagac aagtcgattt ttaacaatgc aacgacagtt agctgagcag ttaaaacaaa
     3721 tatttgatac acatgttgca gataaaatat cagtattccc atcaaatgcc aattttgtac
     3781 ttactaaagg ttcagcagcg caacaattag gacaatacgt atatgaacaa ggatttaaac
     3841 ctcgttttta tgatgagccg gtgatgaagg gctatgtaag atactcaatt gcaaccgaac
     3901 cacagttaaa ccaattagaa gaaattgtta aagaatggag cgcaaaatat gatttatcaa
     3961 aaacaacgaa acacagctga aacacaacta aatatttcaa tatcagatga ccagtcacca
     4021 tcgcatatta atacaggtgt gggcttttta aatcatatgt taacattgtt tacatttcat
     4081 agcggtctgt cattaaacat tgaggcacaa ggtgatatcg atgtagatga tcaccatgta
     4141 actgaagata tcggcattgt tattggccaa ttgttacttg aaatgattaa agataaaaag
     4201 catttcgttc gttatggagc gatgtacatt ccaatggatg aaacattagc acgtgtcgtt
     4261 gtggatataa gtgggcgtcc atacttatct ttcaatgcat cattaagtaa agaaaaagtt
     4321 ggcacgtttg atacggagtt agtagaagaa ttttttagag cagtcgtaat caatgcaaga
     4381 ttaacaacgc atattgattt aattcgtgga ggtaatacac accatgaaat tgaagctata
     4441 ttcaaagcgt tttcccgttc attaggcata gcgctaactg caactgatga tcagcgtgtg
     4501 ccgtcatcga aaggtgtgat tgaatgattg tcatcgttga ttatggatta gggaatatta
     4561 gtaatgtaaa acgcgctatt gaacatttgg ggtatgaggt ggttgtctca aataaccaaa
     4621 atataattga tcaagcagaa acaatcatat tgcccggtgt cggccatttt aaagatgcga
     4681 tgtcagagat aaaacgatta aatctcgatg caatattggc taagaatact gataagaaaa
     4741 tgatcggtat ttgtctagga atgcagttaa tgtatgagta tagtgatgaa ggcgatgcat
     4801 ctggattagg gtttatccca ggaaatattt cgcgtatcca aacagaatac ccagtgccgc
     4861 acttaggttg gaataattta gtgagtaagc atcctatgtt aaatcaagat gtttacttcg
     4921 tacattctta ccaagcgccg atgtcagaaa atgtcattgc atatgcgcag tatggtactg
     4981 atattccagc tattgtccaa tttaacaatt atattggtat tcaattccat cctgaaaaaa
     5041 gcggtacata tgggttacaa attttgcgtc aggcaataca agggggattt ataaatgatt
     5101 gaattatggc cagcgattga tttgattggg tcaacaagtg tgaggttaac agagggtaaa
     5161 tatgatagtg aagaaaaaat gtcgcgttcg gctgaagaaa gtattgctta ctatagtcga
     5221 tttgaatgtg tgaatcgtat tcatattgtc gacttgatag gtgctaaggc acagcatgcc
     5281 cgagagtttg attatattaa gtcattgagg agattaacaa cgaaagatat tgaagtaggt
     5341 ggtggcattc gcacgaagtc acaaatcatg gactactttg ccgcaggaat taattattgc
     5401 atagttggaa cgaaaggtat tcaagatact gattggctta aagagatggc acatacattt
     5461 ccaggtcgca tttatttatc tgttgatgct tatggagaag atattaaagt gaacggatgg
     5521 gaagaggaca cagagttaaa tttatttagt tttgtgagac ggttatcgga tatacctctt
     5581 ggcggcatta tatatactga tattgctaaa gatggcaaaa tgtccggacc taactttgaa
     5641 ttaactggtc aattagtaaa ggcaacgacg attcctgtca ttgcttccgg tggtattaga
     5701 catcagcaag atattcaacg attagcgtcg ctaaatgttc acgctgccat tataggaaag
     5761 gctgcacatc aagcatcttt ttgggagggg ctaaaatgat taaaaaacgt atcattccat
     5821 gtttagatgt caaagatggt cgtgtcgtta aagggattca atttaaagga ttaagggata
     5881 ttgggaatcc tgttgattta gcaatgtatt acaatgaagc gggtgctgat gaattagtat
     5941 ttttagacat ctctaagacg gaagagggtc atagcttaat gctagaagtg attgaacaga
     6001 cagcgtcacg cttgtttatc cctcttactg tagggggtgg gattcaaagt ctcgatgata
     6061 ttacccaatt gttaaatcat ggtgcagata aagtatcatt aaattcaagt gctttaaaaa
     6121 atccacagct cattaaacaa gcgagtgata aattcggtag acaatgcatc tgcatagcaa
     6181 ttgatagcta ttatgatcct gaaagaaaag cacattattg ttgtacgcat ggtggtaaaa
     6241 agatgacaaa tattaaagta tatgactggg tacagcaagt agaacagtta ggtgcaggtg
     6301 agctcctcgt tacaagtatg ggacatgatg gtatgaaaca aggctttgat attgagcatc
     6361 ttgcaaaaat aaaatccctt gtaaatattc caatcattgc ttctggtggt ggtggcaatg
     6421 cacaacactt tgtagaatta tttgatcaga cggatgtttc tgcaggttta gctgcaagta
     6481 tattacatga tcgagaaacg acggttcaat ctattaaaga agtgatacgg caaggaggta
     6541 tagcagtaag atgaccaatt acaaaattga ttttagcaaa ggtttagtgc cagcaatttt
     6601 acaagataat caaacaaaac aagtattgat gttaggttat atgaatcaag aagcttttga
     6661 taaaacgata gaagatggtg tagtatgttt ctattcgcgg tcgaaacaac gtctatggac
     6721 aaaaggtgaa acatctggtc atacgcaact tgttaaagat attcatgtag attgcgacaa
     6781 tgatactatt ttaattgatg tcataccaaa tggaccaaca tgtcatacag gcagtcaaag
     6841 ttgtttcaac acagaagttc cattttcagt gcaaacatta gcgcagacag ttcaagatag
     6901 tgcccaatcc aataatgaaa agtcatatac aaaatattta ttaacagaag gtatagaaaa
     6961 gattacgaaa aaatatggtg aagaagcttt tgaagtcgta attgaagcaa ttaaaggtga
     7021 caaaaaagca tttgtaagtg aagtagcaga tgaactttat catttatttg tcttgatgca
     7081 tgcgctaggc gtcgattttt cagaaattga ggcagaatta gcgcgtagac atcataagcg
     7141 caataacttt aaaggcgaac gacaaaatat cgaacagtgg taaaacaagt atgggctgag
     7201 atatagagaa aaggatcatg tcttatacac ttacaaatat tgtggaaaac gtgacatttt
     7261 caagtttaaa atacgacacc aacatatttt aactatgaat gcggtactaa agttgcgaac
     7321 gcgttataga taagtagtgg ataatcacaa tacgaaacca aaaataattt gaaaagtaaa
     7381 ttgagcaact caggaataga tgtcactgtt aaagatgttg aaaagtatat gaatcgatat
     7441 aatgaagtta tgaagggaaa aaatggcgaa aaagctaaaa agttatattt gtcgttacta
     7501 cctattaata tcatagttgt ctttatattc tttgtattta tactgtgaat aaaaatatat
     7561 ctagcctgaa atagaaatgt catagcctat ttaaaagaca atctccatta gaactaagat
     7621 gtgcatcccg aaagttggac taaacaacta actttatggg atgtattttt atgcaaatca
     7681 tcataaattc gagattaagt taaagttagt tcaagagtaa ttaaacaata aattaaaaat
     7741 agtaggatac ttactttgag ggaagaaaat taactgtata tatttagttt aggaacaagt
     7801 attacggttt atcctgatac aattattgtg gatgggatga tatttttagg tttaaaatac
     7861 gacaccagca aacatcataa ctgtgatagc tcataaatct ccacatatag ctaatctaaa
     7921 aaaataatac atcattggaa tcaagcccca agcatgtaaa tattaaaaat cacaaaagat
     7981 atatgtaaaa aagttacaat ttgcataatt aaattgtgtc taattattga ctaattaaat
     8041 tttgccaaat ataatattaa ttaataattt gaaatgatta gcgtatacac tttaaattct
     8101 ctttggagaa tatatttttt aaatacaaat gtaaacgctt tctcgtcaaa ttaaacaata
     8161 gaaaggatgg tcattatgag tgcttggtta agtaaattat ttgagtttat tcctcgaata
     8221 attatcaatt tgtttatcta aaataaaaaa atagaggtgc tgacaatgat gaaaagtcaa
     8281 aataagtata gtattcgtaa atttagtgta ggtgcatctt ccattttaat agctacatta
     8341 ctatttttaa gtggtggaca agcacaagca gctgagaagc aagtgaatat gggaaattca
     8401 caggaggata cagttacagc acaatctatt ggggatcaac aaactaggga aaatgctaat
     8461 tatcaacgtg aaaacggtgt tgacgaacag caacatactg aaaatttaac taagaacttg
     8521 cataatgata aaacaatatc agaagaaaat catcgtaaaa cagatgattt gaataaagat
     8581 caactaaagg atgataaaaa atcatcgcgt aataataaaa atattcaacg agatacaaca
     8641 aaaaataaca atgctaatcc tagcgatgta aatcaagggt tagaacaggc tattaatgat
     8701 ggcaaacaaa gtaaagtggc gtcacagcaa cagtcaaaag aggcagataa tagtcaagat
     8761 tcaaacgcta ataacaatct accttcacaa agtcgaacaa aggaagcacc atcattaaat
     8821 aagttagatc aaacaagtca acgagaaatt gttaatgaga cagaaataga gaaagtacaa
     8881 ccacaacaaa ataatcaagc gaatgataaa attactaact acaattttaa caatgaacaa
     8941 gaagtgaaac ctcaaaaaga cgaaaaaaca ctatcagttt cagatttaaa aaacaatcaa
     9001 aaatcaccag tagaaccaac aaaggacaat gacaagaaaa atggattaaa tttattaaaa
     9061 agtagtgcag tagcaacgtt accaaacaaa gggacaaagg aacttactgc aaaagcgaaa
     9121 gatgatcaaa cgaataaagt tgccaaacaa gggcagtata aaaatcaaga ccctatcgtg
     9181 ttagtgcatg gtttcaatgg atttacagat gatattaatc cttcagtgtt agctcattat
     9241 tggggcggta ataaaatgaa cattcgccaa gatttagaag aaaatggtta caaagcttat
     9301 gaagcaagta taagtgcttt tggaagtaac tatgaccgcg cagttgaact ttattattat
     9361 atcaaaggcg gtcgtgtaga ttatggtgca gcacatgcag caaaatatgg acatgaacgt
     9421 tatggaaaaa catacgaagg aatttacaaa gactggaaac caggacagaa ggtacaccta
     9481 gttggacata gtatgggtgg tcaaacgata cgtcaactag aagaattact gcgtaatggt
     9541 agtcgtgaag aaatagagta tcaaaagaaa catggtggcg aaatttctcc actattcaaa
     9601 ggtaataatg acaatatgat ttcatcaatt actactttag gaacgccaca taatggaacg
     9661 catgcttcag atttagctgg taatgaagct ttagtgagac aaattgtatt tgatatcggt
     9721 aaaatgtttg gtaataaaaa ctctagagta gacttcgggt tggctcaatg gggtctaaaa
     9781 cagaagccaa atgaatcata cattgattat gtcaaacgcg ttaaacaatc taatttatgg
     9841 aaatcaaaag ataatggatt ttacgatctg acgcgtgagg gtgcaacaga tttaaatcgt
     9901 aaaacgtcgt tgaaccctaa cattgtgtat aaaacataca ctggtgaagc aacgcacaaa
     9961 gcattaaata gcgatagaca aaaagcagac ttaaatatgt ttttcccatt tgtgattact
    10021 ggtaacttaa tcggtaaagc tactgaaaaa gaatggcgag aaaacgatgg tttagtatcc
    10081 gttatttctt ctcagcatcc atttaatcaa gcttatacaa atgcgacgga taaaattcaa
    10141 aaaggcattt ggcaagtaac gcctacaaaa catgattggg atcatgttga ttttgtcgga
    10201 caagatagtt ctgatacagt gcgcacaaga gaagaattac aagatttttg gcatcattta
    10261 gcagacgatt tagtgaaaac tgaaaaggtg actgatacta agcaagcata agttaaaaag
    10321 taaagggagg aattaacaat gactgcagac ttctttcaat taatcggatc attatttaga
    10381 attctaaaag aattattcaa gtaaaacctt ggcggggccc caacaaagag aaattggatt
    10441 cccgatttct acagacaatg caagttggcg gggccccaac ataaagaatt tcgaaaagaa
    10501 attctacaaa caatgcaagt tggggtggga cgacgaaata aattttgcga aaatatcatt
    10561 tctgccccac tcccattggc atttacgaag tttaaatgtg caattagaat atatgtataa
    10621 caatattaaa cacgcggtaa aacgaagtca gtcaattcaa actgatttcg cccaccgcgt
    10681 gtttttaaca tagcttaatg attaataagc attaatgttc aatttatatg gttgttttcc
    10741 aataataaac ctaaagatat agaattcacg caatatcata ccgacaccta tacataatcc
    10801 taaaatgaat agtagtgata tcgctagaaa gaccattgta ttatcctcaa atatatttgt
    10861 atatgcaaac aatgagtcta gaatgattgg atgtaataaa taaataaaga atgagaaagc
    10921 actaatcatt tgaatcgtat taaataacat tgttttaaaa tgcgtgcaaa tacccaagat
    10981 aacaataaac ataatactat tatatggtgt taatgaatat gaaaagctgg taacattcca
    11041 atagtctcca tttgctaacg caataaacac aaaataagta gctacagcta atacaatcat
    11101 aataactaaa taacgttcta agaaatttaa aacacgttcg tagttataac ccatatatgc
    11161 acctaagaag aaataaaaaa tccatccgaa tattatagtg ttttcactta atggataata
    11221 gtgtagcacg gtatcgtgaa acgctgtgtt gttcgtaaag taatataaaa atgattgctg
    11281 taaaataaaa gataacaata ataatatttt actattgaat aggttatagt taattttaaa
    11341 aatgatataa ctcaaaataa agaattgcat gataacaacg ataaaatagc cataccattg
    11401 acctaatagg acattttcaa tgaattgttt attgaaactt gaatctgtta ataatgattc
    11461 actataactg taaaacaatc ccattaatat gtaaggaata agtatatatt ttacgcgtgt
    11521 agttaagtat ctataggtga ctttttgata gtttaaagtc gtcaataact gcgacaaaat
    11581 aataaaacat ggtgtaccaa aaatgacaat attacgaata tagaactgta gtactaaaga
    11641 tcctccttcc atattctcat gttttaaagt aatttgtgtt aataaatgtg taacaataat
    11701 aattgcacat ataatagcac gtaaatatac gagttcaagt ctaatctttt tcatggaatc
    11761 cgtcccatct cttaattaaa tgctcaaaag catcatcact aattagtatt ctagggatgt
    11821 aataatcatt ggagttcgga gtgactgctt tttcctctaa tgaaaaaccg tattttaacc
    11881 cagctttttt gattactggt aatttatcgt tattcatcaa gccataagga taggctatag
    11941 ttttctgcga ctttttaaag tttttagtta gatatttttc acttttgttt aaatctttta
    12001 tgattgtagc ttcagaagat ttcattaatt ttgacttatt atttttagat aagttatgca
    12061 aatcgtgggt atgtgtttca aattcccata acccagtttt atacatttct tttagttctt
    12121 ttttactaat catatcgagg ttgtgaaagt tttcttcccc aacatgacct gtgataataa
    12181 acccagttgc cggtatttta tattttttta agattggata agcattttca taaatagttt
    12241 catccatatc atcaaagtta atccatacac ttcgttttgg aaacttacct tttttcttgt
    12301 aatataaaaa ttctttcaag gttaaaaatt tagcatcatg tgattttagc cattttattt
    12361 gagattcaaa ttgtgattga ctaacactat aatttttaat ttctttacta ctagaaaaga
    12421 aataaataaa attattcaga aaattcgctt ttcttacacg gtgataattt aatgccagag
    12481 cactattttc tttatatttc agttttttag gtgaatcgtc atctgcattt gcaatatgat
    12541 gaccatccag tgtgcttaca ggcaatatga tcaagatact caacactaaa attattaatt
    12601 ttctatactt cacgattctc ttcctctctg ccatttttga atcaatatgc taattgtaaa
    12661 aaatacaaaa atgataatcg cgaaaatgcc catagtttca aatatatcta aaatttcagt
    12721 attttcaatg tttaaagcaa cacgtattgt attgatactt tcgtcatgaa tttcaaatat
    12781 agtaccaata taaacgagta gaacaactaa acaatatatc caaaagacac acgatatagc
    12841 gataagtgct gtttctctta caatatttag cgatgatttt agcgttgggt attccctctg
    12901 tctgggcttg accatgttgc gtaaccacct ttcttacgtt ttaatgcttt tggaaatgcg
    12961 acaagaacta ctgctgcgtt aataatccag tatactgtcg gataccaact tacaaatatg
    13021 agtccagcca tattcttttt ctcgtagcga ctatcaataa agagtgcgac tgtaaattga
    13081 ataacgttta taaaagtcat agtaaatgat gatagtagaa atattgaaaa actatatgtc
    13141 ataaatgtat agtctaagaa gtttgctgtt atgaacaaat agcctaaata tagaagcact
    13201 atatataccc ataaaattga gatgatttgc tcaaacatca aaatatataa aggaaacctt
    13261 ttcgttttca ttgtgctaaa aaagtctcgt agtaatactt cgtgtccccc ttgagcccat
    13321 ctcacgcgtt gcttccaaag acctcccaat gtttctggaa ccaacatcca acacatggca
    13381 agcggttcat acttaatacg atatccacgt aaatgcaatt tccaagaaac tgcaatatct
    13441 tcggtaatca tatcagtatc ccagtagcca acgtcgacaa ctgcactttt tttaaataga
    13501 gtgaagacac ccgaaatagt attgactgcg ccagcaagtg tctgacttcg cttaatacag
    13561 ccaattaaac ttgcatattc tatcgtttga attttaccta aaatagaact cttatttcga
    13621 attctaggat tacctgtaac tgcaccaagt tttggatcat gtttgaaatt ctcaatcata
    13681 taatatggtg catcttgatc aacgatagta tctgcatcca agcacattac ataatcatat
    13741 gaagcctgtt taatgccttg attgagtgcg ttggctttac ctctgttttc ttgtaaatcg
    13801 acgaaaataa agtcattatt ttctttgatt ttatagatga gttctgctgt attatctgaa
    13861 cttccatcat taatgataat aatttctttt ttctcgtatt tgagtgcaag aacattagac
    13921 aacgtatctt caatcgtttc actttcgtta taacaggcaa gtaaaaatgt aatgccttct
    13981 aattcatcca catttatgtc aggcttcttg ttcaatgaat atctaatttc tctggtaaaa
    14041 tagaaataaa ttgaaccgac aatccagtaa atagacataa atacaggata aaaaagcaaa
    14101 aagttaaaaa attgcaattt ctttacctac ctttcgttag ttaggttgta agccatatgg
    14161 taattgatag tattttaatt tgcaatagat tgttgttata attaaacgga aatatttgta
    14221 attgcaactt aattttcctg taacatagtg tgattaattt tcagtagggg ggttttaaaa
    14281 attgaaggat aagattattg ataacgcaat aaccttattt tcagagaagg ggtatgacgg
    14341 tacaacactt gatgatatag ctaaaagtgt aaatataaaa aaagcgagct tatattacca
    14401 ttttgactcg aaaaaaagta tttacgaaca aagtgttaaa tgttgttttg attaccttaa
    14461 taatattatt atgatgaatc aaaataaatc gaactattca attgatgctt tatatcaatt
    14521 cttatttgag tttattttcg acatcgaaga aaggtatatt agaatgtacg ttcaattatc
    14581 taatacgcct gaggaatttt ctggaaatat ttacggacaa atacaagatt taaatcaatc
    14641 attaagtaaa gagatagcaa aattctatga tgaatcaaag ataaagatga caaaagaaga
    14701 ctttcagaat ttaatattgc tgtttcttga aagttggtat ttgaaagcat ctttttcgca
    14761 aaaatttgga gcagtggaag aaagtaaaag tcaattcaaa gatgaagtgt attcgctact
    14821 aaatatattt ttgaagaaat aatttttgtt actagtttgt aataattaac ttacttttgt
    14881 aaaaaaagac atga
//
LOCUS       DAHHOJ010000070        14282 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.070, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000070 DAHHOJ010000000
VERSION     DAHHOJ010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14282
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.070"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..173
                     /locus_tag="NBH32_001762"
     CDS             <1..173
                     /locus_tag="NBH32_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1515355.1"
                     /translation="DNLNSFNKNEIYYIVCAGGVRSAKVVEYLEANGIDAVNVEGGMH
                     AWGDEGLEIKSI"
     gene            499..7068
                     /gene="sasC"
                     /locus_tag="NBH32_001763"
     CDS             499..7068
                     /gene="sasC"
                     /locus_tag="NBH32_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored repetitive surface protein SasC"
                     /protein_id="HCD1515356.1"
                     /translation="MNLLKKNKYSIRKYKVGIFSTLIGTVLLLSNPNGAQALTTDQNV
                     QGESNQALPGNSHNTNVDTSRDITNSSQNTPNQHTTANASTNQALANHHNVGESNQVV
                     PMPVQPSTSSASNNNHSDAKSTATESAANTNNNLSSNNNTLNVPNNTDNNDSARHLTL
                     KEIQEDVRHSSDKPELVAIAEEASNRPKKRSRRAAPADPNATPADPTATPADPAAGNG
                     SAPVAITAPYTPTTDPNANNIGQNAPNEVLSFDDNGIRPSTNRSVPTVTVVDNLPGFT
                     LINGGKVGVFSHAMVRTSMFDSGDAKNYQAQGNVIALGRIRGNDVNDHGDFNGIEKTL
                     TVNPNSELIFEFNTMTTKNYQGMTNLVIKNADNDAVIGEKVVAYGPIWRLFKVPENVS
                     HLKIQFVPKNDAITDPRGIYQLRDGYKYYDFVDSIGLHSGSHVYVERRTMEPTATNNK
                     EFSVTTSLKNNGNFGASFNTDDFVYKIQLPEGVEYVNNSLTKDFQSGNSGVDINDFNV
                     TYDAANRIITIKSTGGGSGNSPARLMPDKILDLKYKLRVNNVPTPRTVTFNDTLTYKT
                     FSQDFINSPAESHTVSTEPYTIDIIMNKDALQAEVDRRIQQADYTFASLDIFNDLKRR
                     AQTILDENRNNVPLNKRVSQADIDSLANQMQHTLIRSVDAENAVNRKVDDMEDLVNQN
                     DELTDEEKQAAIQVIEEHKNEIIGNIGDQTTDDGVTRIKDQGIQTLSRDTATPVVKPN
                     AKQAIRDKAAKQREIINNTPDATQDEIQDALNQLTTDETDAIDNVTNATTNADVETAK
                     NNGINTIGAVVPQVTHKQAARDAINQATATKRQQINSNREATQEEKNAALNELTQATN
                     HALEQINQATTNDDVDTAKGDGLNAINPIAPVTVVKQAARDAVSHDAQQHIAEINANP
                     DATQEERQAAIDKVNAAVAAANANILNANTNADVEQVKTNAIQGIQAIEPATKVKTDA
                     KNAIDQSAETQHNAIFNNNDATLEEQQAAQQLLDQAVATAKQNINAADTNQEVAQAKD
                     QGMQNIVVIQPATQVKTDARNTVNEKAREVITNINATPGATREEKQEAIDRVNALKNR
                     ALTDIGVTSTTAMVNSIRDDAVNQIGAVQPHVTKKQTATGVLNDLATAKKQEINQNTN
                     ATTEEKQIALNQVDQELAAALNNINQADTNAEVDQAQQLGAQAINAIQPNIVKKPAAL
                     AQINQHYNAKLAEINATPDATDDEKNAAINTLNQDRQQAIESLNQANTNNEVDQAAIT
                     AENNIDAVQVDVVKKQAARDKITAEVAKRIEAVKQTPNATDEEKQAAVNQINQLKDQA
                     FNQINQNQTNDQVDATTNQAINAIDNVEAEVVIKPKAIADIEKAVKEKQQQIDNSIDS
                     TDNEKEVATQALAREKEKALVAIEQAQTNEQVNQAATDGITAIKIIQPETRVKPAARE
                     KINQKANELRAQINQDKEATAEERQAALDKINDLVTQAMTNITNDRTDQQVNDSTNQA
                     LGDIALVTPDHIIRAAARDAVKQQYEAKKQEIEQAEHATDEEKQVALNQLANNEKRAL
                     QNIDQAIANNDVKRVESNGIATLKGVEPHIVVKPEAQQAIKASADNQVESIKDTPHAT
                     TDELDEANQQINDTLKQGQQDIDNTTQDVAVNDVRNQTIKAIEQIKPKVRRKRAALDN
                     IDESNNNQLDAIRNTLDTTQDERNIAIAELNKIVNTIKNDIAQNKTNAEVDQTEADGN
                     NNIKVILPKVQVKPAARHSVSAKAEAQNALIDQSDLSTEEERLAAKHLVDQALNQAID
                     QINHADKTAQVNQNSIDAQKIISQIKPATTVKTTALQQIQNIATNKINLIKANNEATD
                     EEQNAAIAQVEQALIKAKQQIASAVTNADVAYLLHDGKNEIREIEPVINRKSTAREQL
                     TTLLNDKKQAIEANVQATVEERNSILAQLQNIYDTAIGQIDQDRSNAQVDKTASLNLQ
                     TIHDLDVHPIKKPDAEKTINDDLARVTHLVQNYRKVSDRNKADALKAITALKLQMDEE
                     LKIARTNADVEAVLKRFKVALGDIEAVITEKENSLLRIDNIAQQTYAKFKSIATPEQL
                     AKVKALIDQYVADGNRMIDEDATLNDIKQHTQFIVDEILAIKLPAEVTNVSPKVGQPA
                     PKVCTPIKKEDKQEVHKVEKELPNTGSEEMDLPLKELALITGAALLARRRTKNEKES"
     gene            complement(7185..8453)
                     /locus_tag="NBH32_001764"
     CDS             complement(7185..8453)
                     /locus_tag="NBH32_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1515357.1"
                     /translation="MYQTIIIGGGPSGLMAAVAASEQSSNVLLIEKKKGLGRKLKISG
                     GGRCNVTNRLPYAEIIKNIPGNGKFLYSPFSIFDNESIIDFFESRGVKLKEEDHGRMF
                     PVSNKAQDVVDTLVTTIERQHVTIKEEEAVSRIEVKEDQTFTVHTQNNSYESHSLVIA
                     SGGTSVPQTGSTGDGYKFAQDLGHTITELFPTEVPITSAEPFIKSNRLKGLSLKDVEL
                     SVLKKNAKKRISHQMDMLFTHFGISGPAALRCSQFVYKEQKNQKTQHISMAIDAFPEL
                     NHEQLKQHITSLLSDTPDKIIKNSLHGLIEERYLLFMLEQAGIDENTTSHHLSNQQLN
                     DLVNMFKGFVFKVNGTLPIDKAFVTGGGVSLKEIQPKTMMSKLVPGLFLCGEVLDIHG
                     YTGGYNITSALVTGHVAGLYAGHYAHASME"
     gene            8859..10520
                     /locus_tag="NBH32_001765"
     CDS             8859..10520
                     /locus_tag="NBH32_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HCD1515358.1"
                     /translation="MSESKEMVRGTFLITISILITKVLGVLFIIPFNYLIGGQENMAP
                     FTYAYAPYNIAIAVATAGVPLAASKYVAKYNAIGAYKVSQKFYKSSFIVMSITGVLGF
                     LVLYFLAPYISELTLARNVHDKNGWSVDDITWIIRIISMVVIFIPVLATWRGIFQGYK
                     SMGPTAVSEVTEQIARVIFILIGSYLVLNVFDGSILLANGIATFAAAVGAIIGIFTLW
                     YYWRKRKHNIDRMVESDYTDIDVSYGKMYKEIIAYSIPFVIVSLNYPLFNLVDQFTHN
                     GALSLVGIPSQLQDIFFNMLNMSTNKIVMIPTSLSAGFAVSLIPYITKTFAEGRLHEM
                     HHQIRTSIGVLMFITVPASIGIMALAQPLFTVFYGYDPIVLGHDPNHDGSRLLFYYAP
                     VAILISLLSVTASMLQGIDKQKLTVYVILASVVIKLALNYPLIMLFHTPGAILSTSIA
                     LLFAIGCNFYILKKYAKFKFSYSWIHFAKIFLYSFIMMLGVELVFFLANLFLEPTKLG
                     YLIIIILGVTVGILIYGTITIKTRLADEFLGEIPEKLRRRVRFLR"
     gene            10517..11212
                     /locus_tag="NBH32_001766"
     CDS             10517..11212
                     /locus_tag="NBH32_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HCD1515359.1"
                     /translation="MRIDKFLANMGVGTRNEVKQLLKKGFVNVNEQVIKSAKTHIEPE
                     NDKISVRGELIEYVENVYIMLNKPKGYISATEDHHSKTVIDLIPEYQHLNIFPVGRLD
                     KDTEGLLLITNDGDFNHELMSPNKHVSKKYEVISANPITEDDIQAFKEGITLSDGKVK
                     PAILTYIDNQTSHVTIYEGKYHQVKRMFHSIQNEVLHLRRIKIADLELDSNLDSGEYR
                     LLTENDFDKLNYK"
     gene            11229..11651
                     /locus_tag="NBH32_001767"
     CDS             11229..11651
                     /locus_tag="NBH32_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1515360.1"
                     /translation="MAKGNLFKAILGIGGAVAAVLVTRKDSRDKLKAEYNKYKQDPQS
                     YKDNAKDKATQLGTIANETIKEVKTNPKEYANRLKNNPKAFFEEEKSKFTEYDNKTDE
                     SIEKGKFDDEGGVAPNNNLRIVTEEDLKKNKNALSDKE"
     gene            11854..13263
                     /gene="sapep"
                     /locus_tag="NBH32_001768"
     CDS             11854..13263
                     /gene="sapep"
                     /locus_tag="NBH32_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mn(2+)-dependent dipeptidase Sapep"
                     /protein_id="HCD1515361.1"
                     /translation="MWKEKVQQYEDQIINDLKGLLAIESVRDDAKASEDAPVGPGPRK
                     ALDYMYEIAHRDGFTTHDVDHIAGRIEAGKGNDVLGILCHVDVVPAGDGWDSNPFEPV
                     VTEDAIIARGTLDDKGPTIAAYYAIKILEDMNVDWKKRIHMIIGTDEESDWKCTDRYF
                     KTEEMPTLGFAPDAEFPCIHGEKGITTFDLVQNKLTEDQDEPDYELITFKSGERYNMV
                     PDHAEARVLVKENMTDVIQDFEYFLEQNHLQGDSTVDSGILVLTVEGKAVHGMDPSIG
                     VNAGLYLLKFLASLNLDNNAQAFVAFSNRYLFNSDFGEKMGMKFHTDVMGDVTTNIGV
                     ITYDNENAGLFGINLRYPEGFEFEKAMDRFANEIQQYGFEVKLGKVQPPHYVDKNDPF
                     VQKLVTAYRNQTNDMTEPYTIGGGTYARNLDKGVAFGAMFSDSEDLMHQKNEYITKKQ
                     LFNATSIYLEAIYSLCVEE"
     gene            13267..14115
                     /gene="dat"
                     /locus_tag="NBH32_001769"
     CDS             13267..14115
                     /gene="dat"
                     /locus_tag="NBH32_001769"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino-acid transaminase"
                     /protein_id="HCD1515362.1"
                     /translation="MEKIFLNGEFVSPSEAKVSYNDRGYVFGDGIYEYIRVYNGKLFT
                     VTEHYERFLRSANEIGLDLNYSVEELIELSRKLVDMNQIETGAIYIQATRGVAERNHS
                     FPTPEVEPAIVAYTKSYDRPYDHLENGVNGVTVEDIRWLRCDIKSLNLLGNVLAKEYA
                     VKYNAVEAIQHRGETVTEGSSSNAYAIKDGVIYTHPINNYILNGITRIVIKKIAEDYN
                     IPFKEETFTVDFLRNADEVIVSSTSAEVTPVIKLDGEPVNDGKVGPITRQLQEGFEKY
                     IESHSI"
BASE COUNT         5355 a         2179 c         2608 g         4140 t
ORIGIN      
        1 cggataattt aaattcattt aataaaaatg aaatatatta tattgtatgt gctggtggag
       61 ttcgaagcgc taaagttgta gaatatttag aggcaaatgg cattgatgcc gtaaatgtcg
      121 aaggcggcat gcacgcatgg ggcgatgaag gtttggaaat aaaaagtatt taaagtagtg
      181 acataattta aaataatatt acatttgtaa tgacaccaag taacgtttcg gttgcttggt
      241 gttttttggt atgaattact ttctgttaca aaacattcta aagcgttctt gttatgtttt
      301 attaagattt taattacaaa acggaaacta aattgtaata aaataaaact ttattttata
      361 aaatgatgat gataaaattg agtgaactta aaatattgta caaaataata tagctataaa
      421 cataatatga gggagcgtat atttttagca taattcttaa caacacagca gagaacagac
      481 aaccaggagg aaaatgaaat gaatttgtta aagaaaaata aatatagtat taggaagtat
      541 aaagtaggca tattctctac tttaatcgga acagttttat tactttcaaa cccaaatggt
      601 gcacaagcct taactacgga tcaaaatgtt caaggtgagt caaatcaagc attacctgga
      661 aactcacata atacaaatgt cgatactagt agagatatta caaatagttc gcaaaataca
      721 cctaatcaac atacgacggc aaatgcatca acaaaccaag ctttggctaa tcatcataac
      781 gttggtgagt caaatcaagt tgtgccaatg ccagtacagc ctagtacgtc atctgcatca
      841 aacaataatc attctgatgc taagtcaaca gcaactgagt cagcggcgaa tacaaataat
      901 aatttatcat caaataacaa tacattaaac gtgcctaata atacagataa caatgattca
      961 gcgcgtcatc tgactttaaa agaaattcaa gaagatgttc gtcattcttc agataagcca
     1021 gagttagttg cgattgctga agaagcatct aatagaccga aaaaaagaag cagacgtgct
     1081 gcgccagcag atcctaatgc aacaccagca gatccaacgg ctacaccagc agacccagcg
     1141 gcaggaaacg gtagtgcacc agttgcaatt acagcgccat acacgccaac aactgatccc
     1201 aatgccaata atataggaca aaatgcacct aacgaagtgc tgtcatttga tgataacggt
     1261 attagaccaa gtacgaaccg ctctgtgcca acagtaaccg tcgttgataa tttaccaggt
     1321 tttacattaa ttaatggtgg taaagtaggg gtatttagtc atgcgatggt aagaacgagt
     1381 atgtttgatt caggagatgc taagaactat caagcacaag gcaatgtaat tgcgttaggt
     1441 cgtattagag gtaatgatgt aaatgaccat ggcgatttta atggtatcga gaaaacatta
     1501 acagtaaatc cgaattctga attaatcttt gaatttaata caatgactac taaaaactat
     1561 caaggtatga caaatttagt cattaaaaat gctgataatg atgcagttat tggtgaaaaa
     1621 gtagtcgctt atggtccgat ttggcgctta tttaaagtac ctgaaaatgt tagtcattta
     1681 aaaatacaat ttgttcctaa aaatgacgca ataacagatc cgcgtggcat ttatcaatta
     1741 cgtgatggtt ataaatacta tgactttgtt gactctatcg gtctacattc tgggtcgcat
     1801 gtctatgttg aaagacgtac aatggagcca acagcaacaa ataacaaaga attttcagtt
     1861 acaacgtcat taaagaataa tggtaacttt ggcgcttcat ttaatacaga tgattttgta
     1921 tataaaattc aattacctga aggtgttgaa tatgtaaata attcattgac taaagatttc
     1981 cagagcggta attcaggtgt tgatatcaat gattttaatg tgacgtatga cgcagcaaat
     2041 cgaattatta caattaaaag tactggtgga ggttcaggga attcaccggc acgacttatg
     2101 cctgataaaa tattggattt gaagtataag ctacgtgtga acaatgtgcc aacaccaaga
     2161 acagtaacat ttaacgatac gttaacgtat aaaacatttt cacaagattt tattaattca
     2221 cctgcagaaa gtcatacagt aagcactgaa ccgtatacaa tcgatatcat catgaataaa
     2281 gacgcactac aagctgaagt tgatagacgt attcaacaag cggattatac atttgcatca
     2341 ttagatattt ttaatgatct taaaagacgc gcacaaacaa ttttagatga aaaccgtaac
     2401 aatgtacctt taaacaaaag agtttctcag gcagatatcg attcattagc aaatcaaatg
     2461 caacatacgt taattcgaag tgttgacgct gaaaatgccg ttaatagaaa agttgatgac
     2521 atggaagatt tagttaacca aaatgatgaa ctgacagatg aagaaaaaca agcagcgatt
     2581 caagtcatcg aggaacataa aaatgaaatt attggaaata ttggtgacca aacgactgat
     2641 gatggtgtta ctagaattaa agatcaaggt atacagactt taagtagaga cactgcaaca
     2701 ccagttgtta aaccaaatgc taaacaagca atacgtgata aagctgcgaa acaaagagaa
     2761 attatcaata acacaccaga tgctactcaa gatgaaattc aagatgcatt aaatcaatta
     2821 acaacggatg aaacagatgc tattgataat gttacgaatg ctactaccaa tgctgatgtt
     2881 gaaacagcta aaaataatgg tattaatact attggagctg ttgtccctca agtaacacac
     2941 aaacaagcag caagagatgc aattaaccaa gcaacagcaa caaaaagaca acagataaat
     3001 agtaatagag aagcaacaca agaagagaaa aatgcagcat tgaatgaatt aacgcaagct
     3061 acgaaccacg ctttagagca aatcaatcaa gcgacaacca atgatgatgt ggatacagcc
     3121 aaaggtgatg gtctgaatgc cattaatcct attgcgcctg taactgttgt caagcaggca
     3181 gcaagagatg ccgtatcaca tgatgcacaa caacatatcg ctgagatcaa tgctaatcct
     3241 gatgcgactc aagaagaaag acaggcagcg attgacaaag taaatgctgc tgtagctgct
     3301 gcgaatgcta acattttaaa tgctaatacc aatgcagatg tggagcaagt gaagacaaat
     3361 gcaattcaag gtatacaagc cattgaacca gctacaaagg ttaaaacaga tgctaaaaat
     3421 gctattgatc aaagtgctga aacgcaacat aatgcgatat ttaataataa tgatgcgacc
     3481 ttagaagagc aacaagcagc acaacaattg cttgatcaag ctgtagccac agcgaagcaa
     3541 aatattaatg cagcagatac gaatcaagaa gttgcacaag caaaagatca aggtatgcaa
     3601 aatatagttg tgattcaacc agcgacacaa gttaaaacgg atgctcgtaa tactgtaaat
     3661 gaaaaagcac gtgaggtgat aacaaatatc aatgctacac ctggtgcgac tcgagaagag
     3721 aaacaagaag cgatagatcg tgtcaatgca cttaaaaata gagcattaac tgatatcggt
     3781 gtgacgtcta ctactgcgat ggtcaatagc attagagacg atgcagtcaa tcaaatcggt
     3841 gctgttcaac cgcatgtaac taagaagcaa acagctacag gtgtattaaa cgatttagca
     3901 actgctaaaa agcaagaaat taatcaaaat acaaatgcaa caactgaaga aaagcaaata
     3961 gcattaaatc aagtagacca agaattagca gctgcactta ataatataaa tcaagctgat
     4021 acaaacgcgg aagtagatca agcgcaacaa ttaggtgcac aagcaattaa tgcgattcag
     4081 ccaaatattg taaaaaaacc tgcagcatta gcacaaatca atcagcatta taatgctaaa
     4141 ttagctgaaa tcaatgctac accagatgca acagatgatg agaaaaatgc tgcaattaat
     4201 acgttgaatc aagacagaca acaagctatt gaaagtctta atcaagcgaa taccaataat
     4261 gaagtagacc aagctgcgat aacagcagaa aataatattg atgctgttca agttgacgta
     4321 gtgaaaaagc aagcagcacg agataaaatc actgctgaag tggcgaaacg tattgaagcg
     4381 gttaaacaaa cacctaatgc aactgatgaa gaaaagcagg ctgctgttaa tcaaatcaat
     4441 caacttaaag atcaagcatt taaccaaatt aatcaaaacc aaacaaatga tcaggtagac
     4501 gcaactacaa atcaagcgat aaatgctata gacaatgttg aagctgaagt agtaattaaa
     4561 ccaaaggcaa ttgcagatat tgaaaaagct gttaaagaaa agcaacagca aattgataat
     4621 agtattgatt caacagataa tgagaaagaa gttgctacac aagcattagc tagagaaaaa
     4681 gaaaaagccc ttgtagcaat tgaacaagct caaacgaacg agcaagtgaa tcaagcggca
     4741 acagatggta taactgcgat aaaaattatt caacctgaaa ctcgagttaa accagcagca
     4801 cgtgaaaaaa tcaatcaaaa agcgaatgaa ttacgtgcgc aaatcaatca agataaagaa
     4861 gcgacagcag aagaaagaca agcggcgtta gataaaatca atgatttagt tactcaagct
     4921 atgacaaata tcacgaatga tagaacagat cagcaagtta acgactcaac aaatcaagcg
     4981 cttggagaca ttgcattagt gacgcctgac catattatta gagcagctgc tagagatgca
     5041 gtaaagcaac aatatgaggc taaaaagcaa gaaatagagc aagcggaaca tgcgactgat
     5101 gaagaaaaac aagttgcttt aaatcaatta gcgaataatg aaaaacgtgc attacaaaac
     5161 atcgatcaag caatagcgaa taatgatgtg aaacgtgttg aatcaaatgg tattgctacg
     5221 ttaaaaggcg tagaaccgca cattgtggtt aaacctgaag ctcaacaagc cataaaagcg
     5281 agcgcagata atcaagtaga atctataaaa gatacaccac atgctacgac tgatgaatta
     5341 gatgaagcaa accaacaaat aaacgacaca cttaaacaag gtcaacaaga tatagacaat
     5401 acaacacaag atgtagctgt aaatgatgtt agaaatcaaa cgattaaggc aatcgaacaa
     5461 attaaaccga aagttagacg caaacgtgca gcgttggata acattgatga aagtaataat
     5521 aaccaactcg atgcaatccg aaatacgcta gatactacgc aagatgaacg aaatattgct
     5581 attgctgagt taaataaaat tgtaaatact atcaaaaatg acattgcgca aaacaaaacg
     5641 aatgcagaag tggatcaaac tgaggcggat ggtaacaata acatcaaagt gattttacct
     5701 aaagttcaag ttaaaccagc agcgcgtcat tctgttagcg caaaagctga agctcaaaat
     5761 gcacttattg atcaaagtga tttatctacc gaagaagaaa gattagctgc taaacattta
     5821 gtagatcaag cacttaatca ggctattgat cagattaatc atgctgataa gactgcacaa
     5881 gttaatcaaa atagtatcga tgctcaaaag ataatttcac aaattaaacc agcgacaaca
     5941 gttaaaacaa cagcactaca acaaattcaa aatatcgcta caaataaaat aaatttaatt
     6001 aaagcaaata atgaagcgac agatgaagaa caaaatgctg caatagcaca agtagaacaa
     6061 gcattaataa aagcgaaaca acaaattgca agtgcagtga cgaatgctga tgtagcatat
     6121 ctattgcatg atggtaaaaa tgaaattcgt gaaattgaac ctgttattaa tagaaaatca
     6181 actgcgcgag aacaattaac aacattgctc aatgataaaa aacaagcaat tgaagcgaat
     6241 gttcaagcaa cggtagaaga aagaaatagt attttagcgc agttacaaaa catttatgac
     6301 actgctattg gacaaattga tcaagatcgt agcaatgcac aagttgataa aacagcatca
     6361 ttaaatctac aaacaataca tgatttagat gtacatccta ttaaaaagcc agatgctgaa
     6421 aaaacgatta atgatgatct tgcacgtgtt acacatttag tgcaaaatta tcgaaaagta
     6481 agtgatcgta ataaggctga tgcattaaaa gctataactg cattaaaatt acaaatggat
     6541 gaagaattaa aaatagcacg cactaatgct gatgttgagg cagttttaaa acgatttaaa
     6601 gttgcattag gcgatataga agcagtaatt actgaaaaag aaaatagctt actgcgcatt
     6661 gataacattg ctcaacaaac atatgcgaaa ttcaaatcaa tcgcaacacc agaacaatta
     6721 gctaaagtaa aagcattaat tgatcaatat gttgcagatg gcaatagaat gattgatgaa
     6781 gatgcgacat taaatgacat caaacaacat acgcaattca ttgttgatga aattttagca
     6841 attaaattgc cagctgaagt gacgaatgta tcaccaaaag tggggcaacc tgctccaaaa
     6901 gtttgtacgc ctattaaaaa agaagataaa caagaagtgc acaaagttga aaaagaactt
     6961 ccaaatactg gttctgaaga aatggattta ccattaaaag aattagcgct aattacaggc
     7021 gcagcattat tagctagaag acgtactaaa aatgaaaaag aatcataatt aacgaaatga
     7081 ccttaatata acgacacttt ttagtgactc agttatatta gggtcatttt taattaaatg
     7141 aaaaagttga tctattaaag agaatcgaac caaattttat actattattc cattgatgca
     7201 tgtgcgtaat gaccggcata taatccagcg acatgtcctg ttacgagtgc acttgtaata
     7261 ttataaccac cagtataacc atgtatatct aatacttcac cacataaaaa taatcccgga
     7321 actaatttag acatcattgt tttaggttga atttctttaa gtgacacacc accacctgtg
     7381 acaaatgcct tatctatagg taatgtcccg tttaccttaa atacaaaccc tttaaacata
     7441 tttactaagt cgttcaattg ttgatttgat aagtgatgtg atgtggtatt ttcatcgatt
     7501 ccagcttgtt ccagcatgaa cagtaagtag cgctcttcaa ttagtccatg caagctgttt
     7561 ttaatgattt tatctggtgt gtccgataat aatgatgtga tgtgttgttt taattgttca
     7621 tggtttaatt caggaaatgc atcgattgcc atagaaatat gctgtgtctt ttgatttttt
     7681 tgttctttat aaacaaactg actacatctt aatgcagctg gaccactgat accaaaatga
     7741 gtgaataaca tatccatttg atgactaatg cgttttttag catttttctt aagtactgac
     7801 aattcaacat cttttaaact taaacctttt agacgattgg atttgatgaa aggttcagct
     7861 gatgtaattg gaacttcggt cgggaataac tccgtaatgg tatgacctaa atcttgtgcg
     7921 aacttataac catcaccagt tgaacctgtt tgagggacac ttgttccacc tgaagcaatc
     7981 actagcgaat ggctttcata actattattt tgagtatgta cagtgaaagt ttgatcttct
     8041 tttacttcga ttctactaac agcttcttct tctttaatcg ttacatgttg gcgttcgata
     8101 gttgtcacta atgtatcaac tacgtcttgt gctttgttgg agactggaaa catacgcccg
     8161 tgatcttctt cttttaattt aacaccccta gactcaaaaa aatctatgat ggattcatta
     8221 tcaaaaattg aaaagggact atataaaaat tttccatttc caggaatgtt cttaataatt
     8281 tcagcatatg gtaatcgatt agttacgtta catctaccgc caccagatat tttgagttta
     8341 cgacctagac ctttcttttt ttcaatgagt aacacattgc tactttgttc gcttgcagct
     8401 actgccgcca ttaagccgct aggtccgcct ccgataataa ttgtttgata catatacgct
     8461 tcctccaaat ttgttactgg acattcaatt gctattttat agtacttttt taaactttgc
     8521 tatatctctt gtgttatgag atgacaatta tgttgaatat atttataatg tgaacttcat
     8581 aaaaatgaag atgtatgtgt atgataagaa tgatttttct actgttatgc gatgttcatc
     8641 tcaaatagta gtataatgtt catatgaaat tttgtaatat tctaatcaaa atcgagtatt
     8701 caatgacaat gcgttgataa ttttgttatg ataattcatt gaatatacga caatgaaata
     8761 aagttttgtg atatattcat aataatgaaa aatgatttta cagagaaata gcttttagtt
     8821 tacaaaactt taataaacat gagataggga gattcgtaat gagtgaaagt aaagaaatgg
     8881 tgcgtggaac ctttttaatt accattagta tattaattac caaggtattg ggcgtacttt
     8941 ttatcattcc attcaactat ttaattggtg gacaagaaaa tatggcgccg ttcacatacg
     9001 cttatgcacc atataatatt gcaattgcag ttgcgacagc aggtgtacct ttagcagctt
     9061 cgaaatatgt tgcgaaatat aatgctattg gggcatataa agtcagtcag aaattttata
     9121 aatcgagttt tattgtaatg agtattaccg gtgttttagg tttcttggta ctgtatttcc
     9181 tagcacctta tatttctgaa ttaacattag ctagaaacgt acatgataaa aacggttggt
     9241 cagtcgatga tattacttgg attataagaa tcattagtat ggttgttatc tttattcctg
     9301 tattagcgac atggagaggt atattccaag gttataaatc tatggggcca acagcagtgt
     9361 cagaagtaac tgagcaaatt gcacgcgtga ttttcatatt aatcggaagt tatttagtac
     9421 taaatgtctt tgatggctct attttattag ccaatggtat tgctactttt gcagcagcag
     9481 ttggtgcgat tattggtata ttcacactct ggtattactg gagaaagcgt aagcataata
     9541 ttgatcgtat ggtcgaatct gattacacag atatagatgt gtcatatggc aagatgtata
     9601 aagaaatcat tgcttatagt attccattcg ttattgttag tttgaattat ccattattta
     9661 acttagtaga tcaatttaca cataacggtg ctttatcttt agttggtata ccttcacagt
     9721 tacaagatat attctttaat atgctaaata tgtcaactaa taaaattgtt atgattccaa
     9781 catcattaag tgctggtttt gcggtgagtt taattcctta tattacaaaa acatttgcag
     9841 aaggtcgctt acatgaaatg catcatcaaa ttagaacgtc aattggagta ttaatgttta
     9901 taactgttcc agctagtatt ggaattatgg ccttggcgca accattattc acagttttct
     9961 atggttacga tccaattgtt ttaggtcatg accctaacca tgatggcagt cgattattat
    10021 tctattatgc gcctgtagca atactaattt cactattaag tgtaacggcg tcaatgttac
    10081 aaggtataga taaacagaaa ttaacagttt atgttatttt agcttctgta gttatcaagt
    10141 tagcgttaaa ctatccatta attatgttat tccatacacc tggtgcaatt ttaagtacta
    10201 gtatcgcatt attatttgca attggatgta atttctatat tcttaaaaaa tatgcgaagt
    10261 ttaaattcag ctatagttgg attcattttg caaaaatatt cttgtattca tttattatga
    10321 tgctaggtgt agaattagta ttcttccttg caaatctatt cttagaacct actaaacttg
    10381 gttacttaat cattattatt ttaggtgtta cagtaggtat tctaatttac ggtacaatca
    10441 ctattaaaac gcgtcttgcg gacgaattct taggtgaaat tcctgaaaaa ttaagacgta
    10501 gagttaggtt tttacgatga gaatagataa atttttagca aatatgggcg tcggtacacg
    10561 taatgaggtc aaacagttac ttaaaaaagg ctttgtaaat gtcaacgaac aagtaattaa
    10621 atcagcaaaa acacatatag aaccagaaaa cgataagata tcagttcgtg gagaattaat
    10681 agaatacgtt gaaaacgtct atattatgtt aaataagcca aaaggttata tatcggcaac
    10741 tgaagatcat cattcaaaaa ccgttattga tttaatacct gaataccaac atctaaatat
    10801 ttttccggtt ggtcgtcttg ataaagatac agaagggcta ttattgataa cgaatgatgg
    10861 tgattttaat catgaattaa tgagtccaaa caaacatgtt tctaaaaaat atgaagttat
    10921 ttcagcaaat cctatcactg aagacgacat tcaagctttt aaagagggta taacactgtc
    10981 tgatggcaag gttaagcctg caattttaac ttatattgat aatcaaacgt cacacgtaac
    11041 aatttatgaa ggaaaatatc atcaagttaa acgaatgttt catagcattc aaaatgaggt
    11101 attacattta agacgtatca aaattgctga tttagaattg gacagcaatc tagattcagg
    11161 agaatatcgt ttattaactg aaaatgattt tgacaaatta aattataaat aaaagggaga
    11221 cgattgatat ggcaaaaggt aatttattta aagcgatttt aggtataggt ggcgctgtag
    11281 cagctgtact tgttacacgt aaagatagtc gtgacaagct gaaagcagaa tataataaat
    11341 ataaacaaga tcctcaaagc tataaagata atgctaagga taaagcgacg caattaggaa
    11401 caattgctaa tgaaacaatt aaagaagtaa aaacaaatcc gaaagaatat gctaatagat
    11461 taaaaaataa tccaaaagca tttttcgaag aagaaaaatc aaaatttacc gaatatgaca
    11521 ataagactga cgaaagtatt gaaaaaggta aatttgatga tgaaggtggc gtagcaccaa
    11581 ataataattt acgtatcgtc actgaagaag acttaaaaaa gaataaaaat gcattatctg
    11641 ataaagaata aggcatacaa ttaataagtt ggtattgaac tggcatatta aatatataaa
    11701 ttaaaaaagg aacaaagcag ctattaatga tttgtgtcat atcgatagca tcgttttgtt
    11761 cctttttatt attttctgac atcttattgt agtttttata tcatttcatg taaaatagaa
    11821 tttaacttat agaaaaaagg aagtcgattt cgaatgtgga aagaaaaagt tcaacaatac
    11881 gaagatcaaa tcattaatga cttaaaagga ttattagcaa ttgaaagtgt gagagatgat
    11941 gcgaaagcat cagaagacgc accagttggt ccaggtcctc gtaaggcatt agactacatg
    12001 tatgaaattg cacatagaga tggatttaca acacatgatg tggatcatat tgcaggaaga
    12061 attgaggcag gtaaaggaaa tgacgtatta ggtatcttat gtcatgttga cgttgttcct
    12121 gctggtgatg gatgggatag taatccgttt gagccggttg taacagaaga tgctatcata
    12181 gctagaggta cacttgatga caaaggtcca acaattgctg cttattatgc aattaagata
    12241 ttagaagata tgaatgtgga ttggaagaaa cgtattcata tgattattgg tacggatgaa
    12301 gaatctgatt ggaaatgtac ggatcgctat tttaaaacag aagaaatgcc aacattaggt
    12361 tttgcaccag atgcagaatt tccgtgtatt catggtgaaa aaggcattac aacatttgat
    12421 ttagttcaaa ataaacttac tgaagatcaa gatgaacctg attatgaatt aataactttc
    12481 aaatctggtg aacgttacaa catggtacct gatcatgcag aagcaagagt gcttgttaaa
    12541 gaaaatatga cagatgttat tcaagacttt gagtactttt tagaacaaaa tcatttacaa
    12601 ggtgatagta ctgttgatag tggcattcta gttttaacag ttgaaggtaa agcggttcat
    12661 ggtatggatc catctatcgg tgtgaatgcg ggtctttact tactaaaatt cttagcatca
    12721 ttaaatcttg ataataatgc acaagcgttt gtagcattta gtaatcgcta cttatttaat
    12781 tcagattttg gtgaaaagat gggaatgaaa ttccatacag atgtcatggg tgatgtgaca
    12841 actaacattg gtgttattac atatgataat gaaaatgcag gtcttttcgg tatcaattta
    12901 agatatcccg aaggatttga atttgaaaaa gctatggatc gttttgcaaa tgagattcaa
    12961 caatatggct ttgaagtgaa attaggtaaa gtccaaccac cacattatgt tgataaaaat
    13021 gatccttttg tacaaaagtt agttactgca tatagaaatc aaacaaatga tatgactgaa
    13081 ccttatacta taggtggcgg tacttatgcg agaaacttag acaagggtgt agcatttggc
    13141 gcaatgttta gtgattctga agatttaatg catcaaaaaa atgaatatat cactaaaaaa
    13201 cagttattta atgcaactag tatttattta gaagcaattt attcattatg tgtggaggaa
    13261 taatatatgg aaaaaatttt tttaaatggt gagtttgtaa gtccaagtga agcaaaggtt
    13321 tcatacaacg acagaggata cgtatttggc gatggtattt atgaatacat tcgagtatat
    13381 aatggtaagt tatttacagt aacagaacat tatgaaagat ttttacgtag tgccaatgag
    13441 attggtttag atttaaatta ttctgtagaa gaattaattg aactatctcg taaattagtt
    13501 gatatgaatc aaattgaaac tggggcaatt tatattcaag caacgcgtgg tgtagctgaa
    13561 agaaatcata gcttcccgac acctgaagta gaaccagcaa ttgttgctta tacaaagagt
    13621 tatgatcgtc cttatgatca tttagaaaat ggtgtgaatg gtgttaccgt tgaagatatc
    13681 cgttggttac gttgcgatat taaaagctta aatttattag gtaatgtttt agcgaaagaa
    13741 tatgctgtga aatataatgc agttgaagca atccaacatc gaggcgaaac tgtcactgaa
    13801 ggatcttcaa gtaatgctta tgcaattaaa gacggtgtga tttatacaca tccgattaac
    13861 aactatattc ttaatggtat tacacgaatt gtaattaaaa aaattgccga agactataac
    13921 atcccattta aagaagaaac gtttactgta gatttcttga gaaatgcaga tgaagttatt
    13981 gtttcaagta cttcagctga ggttacacct gttattaaat tagatggtga accagttaat
    14041 gatggtaaag ttggcccaat tacacgtcaa ctacaagaag gatttgaaaa gtatatagag
    14101 tcacacagta tttaaaattc tttcatcata tttttagatt aaattactta aatttatcaa
    14161 ctttcgtaag tgaattatgt tataatttaa aacgaagctt aaaagtgttg tttagaaaac
    14221 acatattagt aaaaagtgcc attttgtttc tcattttcag ttgaaatata atgataaaaa
    14281 tt
//
LOCUS       DAHHOJ010000071        14263 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.071, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000071 DAHHOJ010000000
VERSION     DAHHOJ010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.071"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..918)
                     /locus_tag="NBH32_001770"
     CDS             complement(<1..918)
                     /locus_tag="NBH32_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCD1515363.1"
                     /translation="MTSLKERDYFFDNARAILILLVVFGHMLQPYTSGDKYLSALYLV
                     IYSFHMPTFLFISGYFAKNIDKPYYLEKISKRLIVPYMIFFAFFSIYYFLTGKSDELQ
                     LDPFNPVFALWFLITLFFFHVILVIVRRFNPYKVLSVSIIISIGAGFSGNIDSYLSIS
                     RTIVFFPIFYLGYIFTKKHTAIFKNKKLIPVSIITFILFFIIYVIHPINADWLLGSSP
                     YTSLENEGQSVFSPFKRLILYGIILIAMTAFLNLISTKKKLYTYIGSRTLYVYLLHGL
                     IIGIVRGFEWYPFDNPISLMTYLYLISISV"
     gene            1071..1490
                     /locus_tag="NBH32_001771"
     CDS             1071..1490
                     /locus_tag="NBH32_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1515364.1"
                     /translation="MYKQLEKLITLTNNDLNLVNRRFGQRTDITSEQLELLRILFNYD
                     RLSQYDLTMKISREQSIVSRWIKKLVLKGYITSQQSSEDLRCKELILTDQARTLISQI
                     NNARCELIEARCQCLSEVELDNLNQLLDKLNQRRISL"
     gene            1517..1627
                     /locus_tag="NBH32_001772"
     CDS             1517..1627
                     /locus_tag="NBH32_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515365.1"
                     /translation="MSLCTQQKLNHELQAKVAVIVKIDGKQSPLFLLVYV"
     gene            complement(1698..5471)
                     /locus_tag="NBH32_001773"
     CDS             complement(1698..5471)
                     /locus_tag="NBH32_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HCD1515366.1"
                     /translation="MAKKFNYKLPSMVALTLVGSAVTAHQVQAAETTQDQTTNKNVLD
                     SNKVKATTEQAKAEVKNPTQNISGTQVYQDPAIVQPKAANKTGNAQVNQKVDTTQVNG
                     DTRETQSTTSNNAKPVTKTTNTTASKTNNNVTSAGYSLVDDEDDNSENQINPELIKSA
                     AKPAALETQYKAAAPKATPVAPKAKTEATPKVTTFSASAQPRSVAAAPKTSLPKYKPQ
                     VNSSINDYIRKNNLKAPKIEEDYTSYFPKYAYRNGVGRPEGIVVHDTANDRSTINGEI
                     SYMKNNYQNAFVHAFVDGDRIIETAPTDYLSWGVGAVGNPRFINVEIVHTHDYASFAR
                     SMNNYADYAATQLQYYGLKPDSAEYDGNGTVWTHYAVSKYLGGTDHADPHGYLRSHNY
                     SYDQLYDLINEKYLIKMGKVAPWGTQSTTTPTTPSKPTTPSKPSTPSTGKLTVDANNG
                     VAQIKPTNSGLYTTVYDKTGKATNEVQKTFAVSKTATLGNQKFYLVQDYNSGNKFGWV
                     KEGDVVYNTAKSPVNVNQSYSIKPGTKLYTVPWGTSKQVAGSVSGSGNQTFKASKQQQ
                     IDKSIYLYGSVNGKSGWVSKAYLVDTAKPTPAPTPKPSTPTTNNKLTVSALNGVAQIN
                     AKNNGLFTTVYDKTGKPTKEVQKTFSVTKEASLGGNKFYLVKDYNSPTLIGWVKQGDV
                     IYNNAKSPVNVMQTYTVKPGTKLYSVPWGTYKQEAGAVSGTGNQTFKATKQQQIDKSI
                     YLYGTVNGKSGWISKAYLAVPAAPKKAVEQPKTAVKAYAVTKPQTTQTVSKIAQVKPN
                     NTGIRASVYEKTAKNGAKYADRTFYVTKERAHGNETYVLLNNTSHNIPLGWFNVKDLN
                     VQNLGKEVKTTQKYTVNKSNNGLSMVPWGTKNQVILTGNNIAQGTFNAAKQVSVGKDV
                     YLYGTINNRTGWVNAKDLTAPTAVKPTTSAAKDYNYTYVIKNGNGYYYVTPNSDTAKY
                     SLKAFNEQPFSVVKEQVINGQTWYYGKLSNGKLAWIKSTDLAKELIKYNQTGMTLNQV
                     AQIQAGLQYKPQVQRVPGKWTDANFNDVKHAMDTKRLAQDPALKYQFLRLDQPQNISI
                     DKINQFLKGKGVLENQGAAFNKAAQMYGINEVYLISHALLETGNGTSQLAKGADVVNN
                     KVVTNSNTKYHNVFGIAAYDNDPLREGIKYAKQAGWDTVSKAIVGGAKFIGNSYVKAG
                     QNTLYKMRWNPAHPGTHQYATDVDWANINAKIIKGYYDKMGEVGKYFDIPQYK"
     gene            complement(5699..6133)
                     /locus_tag="NBH32_001774"
     CDS             complement(5699..6133)
                     /locus_tag="NBH32_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515367.1"
                     /translation="MFLKVNNQKMLEDCFYIRKKVFVEEQGVPEESEIDEYESESIHL
                     IGYDNGQPVATARIRPINETTVKIERVAVMKSHRGQGMGRMLMQAVESLAIDEGFYVA
                     TMNAQCHAIPFYESLNFKMRGNIFLEEGIEHIEMTKKLTSLN"
     gene            complement(6290..6760)
                     /locus_tag="NBH32_001775"
     CDS             complement(6290..6760)
                     /locus_tag="NBH32_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499548.1"
                     /note="Essential protein for resistance to osmotic stress
                     from salt; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic stress response protein"
                     /protein_id="HCD1515368.1"
                     /translation="MSRKTYEKIANINGMFNMLEQQIIHSQDMAHFRSEFFYVNHEHR
                     ENYEALLIYYKNSIENPIVDGACYILALPEIFNSVDVFESELPFSWVYDENGITETMK
                     SLSIPLQYLVAAALEVTDVNIFKPSGFTMGMNNWNIAQMRIFWQYTAIIRKEAL"
     gene            complement(6808..8034)
                     /locus_tag="NBH32_001776"
     CDS             complement(6808..8034)
                     /locus_tag="NBH32_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenyl-teichoic acid--peptidoglycan
                     teichoic acid transferase"
                     /protein_id="HCD1515369.1"
                     /translation="MNKFLKYFLILLALVLIVVPIVFATLLFKTSQDAFESSQDSKNA
                     NRQSNLRDNKVNPEEQPISILFLGIDDNDGRRKKGQDAEHSRSDAMILTTFNQSKHQI
                     RMLSIPRDTISYIPKVGYYDKITHAHAYGGPIAAMDSVEATMNVPVDYYVRVNMKAFV
                     EAVNELGGIYYDVPYDLNEPNTDDTGKIKIKKGYQKLNGDEALAVARTRHHDSDLKRG
                     QRQMELIKILFQKAQEVDSIDKLDNVIQIVGKNAKHNLTNSEIKALAKMYLTNDVEIK
                     TAQLKGKDDMLNGIYYYHPSVDSIQKYANLLRKDLELSPINDKNDFLDQRVINHYGSL
                     IPLTPLDSSLLRKEQNDTTDKDKSSNNSDDSNNSNSNNSVNNQQQPATDQNLNQNQGG
                     TQQTPQASNNQNGVVN"
     gene            8475..9668
                     /gene="fmtA"
                     /locus_tag="NBH32_001777"
     CDS             8475..9668
                     /gene="fmtA"
                     /locus_tag="NBH32_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala esterase FmtA"
                     /protein_id="HCD1515370.1"
                     /translation="MKFNKVKLVIHACVLLFIIISIGLVFHRLQTKTNSIEPIHKETK
                     LSDNAKYLVDRNKGKGEPSKLKEVYNSKDPKYKKIDQYLQNSLFNGSVAVYENGKLKM
                     SKGYGYQDFEKGIKNTPNTMFLIGSAQKFSTGLLLKQLEEEHKININDPVSKYIPWFK
                     TSKPIPLKDLMLHQSGLYKYKSSKDYKNLDQAVRAIQKRGIDPKKYKKHMYNDGNYLV
                     LAKVIEEVTGKSYAENYYTKIGDPLKLQHSAFYDEKSFRKYLAKGYSYNSTGLSFLKP
                     NVLEQYYGAGNIYMTPTDMGKLITQMQQYKLFSPKITNPLLHEFGTKQYPDEYRYGFY
                     VKPTLNRLNGGFFGQVFTVYYNDKYVVVLALNVKGNNEVRIKHIYNDILKQNKPYNTK
                     GVIVQ"
     gene            complement(10011..10289)
                     /gene="qoxD"
                     /locus_tag="NBH32_001778"
     CDS             complement(10011..10289)
                     /gene="qoxD"
                     /locus_tag="NBH32_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit IV"
                     /protein_id="HCD1515371.1"
                     /translation="MKHTVGFIASIVLTLLAVYVTLYTSLTFHAKLTIIFGFAFIQAG
                     LQLLMFMHLTEGKDGRLQTFKVIFALVITLCFVVGTYWVMQGGHSSHL"
     gene            complement(10298..10903)
                     /gene="qoxC"
                     /locus_tag="NBH32_001779"
     CDS             complement(10298..10903)
                     /gene="qoxC"
                     /locus_tag="NBH32_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit III"
                     /protein_id="HCD1515372.1"
                     /translation="MSHDTNTIDSRTHEGELNKLGFWIFITAEFALFGTLFATLLTLQ
                     HGGDYAGKMTTELFELPLVLIMTFALLFSSYTCGIAIYYMRQEKQKLMMFWMIITLLL
                     GLVFVGFEIYEFAHYASEGVNPTIGSYWSSFFILLGTHGCHVSLGIVWAICLLIQIQR
                     RGLDKYNAPKLFIVSLYWHFLDVVWVFIFTAVYMIGMVYSG"
     gene            complement(10893..12881)
                     /gene="qoxB"
                     /locus_tag="NBH32_001780"
     CDS             complement(10893..12881)
                     /gene="qoxB"
                     /locus_tag="NBH32_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001010769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit I"
                     /protein_id="HCD1515373.1"
                     /translation="MNFPWDQLLVKGNWMITMAQIGAPFLVIGLIAVITYFKLWKYLY
                     KEWFTSVDHKKIGIMYLICAVLMFVRGGIDALLIRAQLTVPDNKFLESNHYNEIFSTH
                     GVIMIIFMAMPFIFGLWNIVVPLQIGARDVAFPVLNNVSFWLFFAGMILFNLSFIIGG
                     SPAAGWTNYAPLAGEFSPGPGVNYYLIAIQISGLGTLATGINFFVTILRCKTPTMKFM
                     QMPMFTVTTFITTLIVILAFPPLTVALALMTTDRIFDTAFFTVAHGGMPMLWANFFWV
                     WGHPEVYIVILPAFGIYSEIIPTFARKRLFGHQSMVWATAGIAFLSFLVWVHHFFTMG
                     NGALINSFFSISTMLIGIPTGVKLFNWLLTLYKGRITFESPMLFSLAFIPNFLLGGVT
                     GVMLAMASADYQYHNTYFLVAHFHYTLVTGVVFACLAGLIFWYPKMMGYKLNETLNKW
                     CFWFFMIGFNVCFLPQFILGLDGMPRRLYTYMPSDGWFLLNLISTIGALLMAIGFLFL
                     VVSIVYSHFKSPREATGDNWDGLGRTLEWTTASAIPPKYNFAITPDWNDYDTFVDMKE
                     HGRHYLDNHNYKDIHMPNNTPVGFWIGIFMTIGGFFLIFETVIPALICLFGIFGTMIY
                     RSFQIDHGYHIPAAEVAETEARLREARIKEREAVSHES"
     gene            complement(12881..13981)
                     /gene="qoxA"
                     /locus_tag="NBH32_001781"
     CDS             complement(12881..13981)
                     /gene="qoxA"
                     /locus_tag="NBH32_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome aa3 quinol oxidase subunit II"
                     /protein_id="HCD1515374.1"
                     /translation="MSKFKSLLLLFGTLILLSGCSNIEIFNAKGPVASSQKFLILYSI
                     VFMLVICFVVLGMFAIFIYKYSYNKNAESGKMHHNAIIETIWFVIPIIIVAALAIPTV
                     KTLYDYEKPPKSEKDPMVVYAVSAGYKWFFAYPDEHIETVNTLTIPKDRPVVFKLQAM
                     DTMTSFWIPQLGGQKYAMTGMTMNWTLEASQTGTFRGRNSNFNGEGFSRQTFKVNAVS
                     QKDYDKWVKEVKGKKTLDQDTFDKQLLPSTPNKALEFNGTHMAFVDPAADPEYIFYAY
                     KRFNFELKDPNFTSEENMFKDVSDKPLIPARKAQITNANYKRHGMKLMILGNDEPYNN
                     EFKKDESKNAKEMKKISKDAQDQDNDDHGGGH"
BASE COUNT         4709 a         2258 c         2373 g         4923 t
ORIGIN      
        1 tacagatata cttattaaat aaagatacgt cattaatgat ataggattat cgaatgggta
       61 ccattcaaat cctctaacaa tgccaataat taaaccatgt aataaatata cgtatagcgt
      121 acgactacca atataagtat ataatttttt ctttgttgat attaaattta gaaacgcagt
      181 cattgcgatt aatataattc catataatat aagtcgttta aaaggactga atacactctg
      241 tccttcattt tcaagtgaag tatatggtga acttcctaat aaccaatctg cattgatagg
      301 atgaatcacg taaatgataa aaaacaaaat aaacgtaatg atagatactg gtattagttt
      361 tttattttta aaaatagccg tatgtttttt ggtgaaaatg taacctaggt aaaatattgg
      421 gaaaaatacg attgtccttg aaatgcttaa gtagctatcg atgtttcctg aaaaacctgc
      481 tccaatagat ataataattg aaactgatag cactttatat ggattaaatc ttctaactat
      541 tactaaaatg acatgaaaga aaaatagcgt gatcaaaaac cataacgcaa atactgggtt
      601 aaaaggatca agttgtaatt cgtcactttt acctgttaag aaataatata ttgaaaagaa
      661 tgcaaaaaat atcatataag gtactatcaa acgttttgaa attttttcta aatagtatgg
      721 tttatcaata tttttcgcga aataaccaga tataaacaaa aatgttggca tatggaaact
      781 ataaataact aggtataatg ctgataaata tttatcgcca ctagtgtagg gttgtaacat
      841 atgtccaaat acgactaata atattaaaat tgctcttgcg ttgtcaaaaa aataatctct
      901 ctcttttaat gaagtcatat tttatctcct ttatgtccat aagactctta ctaatttatt
      961 atattttgta ttagttatgc aacaaatttg tctattgtgt cgaatagtat tgtaaaaatt
     1021 ataaaaaaag catataatat tataaataat atgtagtgga gtagaacaac atgtataaac
     1081 aacttgaaaa acttattaca ctgactaaca atgacttaaa cttagtgaat agaagatttg
     1141 gacaacgcac ggatatcaca tctgaacagc tagaacttct ccgtatttta tttaattacg
     1201 atcgcttatc acagtatgat ttgacgatga agattagcag ggaacaatct atagtttcaa
     1261 ggtggattaa gaaattagtt ttgaaagggt acatcacaag tcaacaatct agcgaagatt
     1321 taagatgtaa agaattaatt ttgactgatc aagcacgtac attaatttca caaataaata
     1381 atgcacgttg cgaattgatt gaagcaagat gtcaatgttt atcggaagtc gaattagata
     1441 atttaaatca attacttgat aagttaaatc aacgacgcat atcgttgtaa cacgacaata
     1501 gcaaacacat aatttagtgt cattgtgcac acaacaaaaa ttgaatcatg aattacaagc
     1561 aaaagtagcg gtgattgtta aaattgatgg taaacaatca ccgctatttt tgcttgtgta
     1621 tgtataaaaa agggatcaaa ggtcatcccc catgattgat agtgggggga tgacttttga
     1681 tcctatgttc atgttgctta tttatattgt gggatgtcga agtatttgcc gacttcgccc
     1741 attttatcat agtagccttt gatgatttta gcattgatgt tagcccaatc tacatctgta
     1801 gcatattggt gtgttcctgg atgtgcagga ttccatctca ttttgtaaag cgtattttga
     1861 ccagctttta catatgagtt gccgatgaat ttagcaccac caacgatggc ttttgaaact
     1921 gtgtcccaac cagcttgttt agcatattta ataccttcac gtaaaggatc gttatcatat
     1981 gcagcaatac caaatacgtt atggtatttc gtgttagagt tagttacaac tttgttgttc
     2041 actacatctg caccttttgc taattgagaa gtaccgttac ctgtttctaa tagggcatgt
     2101 gagataagat aaacttcatt aatgccatac atttgagcag ctttgttaaa tgcagcacct
     2161 tggttttcta atacaccttt accttttaag aattgattaa ttttatcaat agaaatattt
     2221 tgtggttggt ctaagcgtaa gaattgatat tttaatgctg gatcttgagc taaacgcttc
     2281 gtatccattg catgcttaac atcattaaag ttagcatctg tccacttacc tggtacacgt
     2341 tgtacttgtg gtttatattg taaaccagct tgaatttgag caacttggtt taatgtcata
     2401 cctgtttgat tatacttaat taattcttta gctaaatcag tcgatttaat ccatgctaat
     2461 ttaccgttag ataatttacc atagtaccaa gtttgtccat taatgacttg ttctttaaca
     2521 actgagaatg gttgttcatt aaatgctttt aatgagtatt tagctgtatc agaatttggt
     2581 gttacatagt aataaccatt accattttta attacataag tgtagttata atctttggca
     2641 gctgatgtag ttggtttaac agcagttggt gcagttaaat cttttgcatt tacccaacca
     2701 gtgcggttat taatagtacc gtataaataa acatctttgc ctacagatac ttgtttcgct
     2761 gcattaaatg taccttgagc aatgttattg cctgttaaaa tgacttggtt tttagtaccc
     2821 caaggaacca ttgataagcc gttatttgat ttattaacag tatatttttg agtcgtttta
     2881 acttctttgc ctaagttttg aacatttaag tctttaacat tgaaccaacc taatggaata
     2941 ttatgacttg tattatttaa taatacatat gtttcattac catgtgcacg ttcttttgtt
     3001 acatagaatg tacgatccgc atattttgcg ccgtttttcg ctgttttttc ataaacagaa
     3061 gcacgaatac cagtgttgtt tggtttaact tgagcaattt tgctaactgt ttgagtcgtt
     3121 tgtggtttag taacagcata agcttttaca gctgtttttg gttgttctac agctttttta
     3181 ggtgcagcag gtacagctaa atatgcttta ctaatccatc cggatttacc atttacagtt
     3241 ccatataaat agatagattt atcaatttgt tgttgcttag tcgctttaaa agtttggtta
     3301 cctgtgccag aaaccgcacc agcttcttgt ttataagtac cccaaggtac tgaatataat
     3361 ttagtgcctg gttttactgt atatgtttgc attacattta caggtgattt tgcattgtta
     3421 taaataacgt caccttgttt aacccaacca attaaagttg gactattgta atctttaact
     3481 aggtagaatt tgtttccacc tagacttgct tcttttgtta cagaaaatgt tttttgaact
     3541 tctttcgttg gcttaccagt tttgtcataa actgtagtga ataaaccatt gtttttagca
     3601 ttaatttgag caacaccatt taatgctgag actgttaatt tattatttgt tgtcggcgtt
     3661 gatggcttag gtgttggtgc aggtgtaggt ttagcagtat caactaaata tgctttactt
     3721 acccaaccag atttaccatt cacagagcca tataaataaa ttgatttatc aatttgttgt
     3781 tgctttgaag ccttaaatgt ttggtttcca gagccagaca cgctaccagc aacttgttta
     3841 gatgtacccc aaggtactgt ataaagtttc gtaccaggtt tgattgaata tgattgattt
     3901 acatttacag gtgatttagc tgtgttgtaa accacatcgc cttctttaac ccaaccaaat
     3961 ttattaccag aattgtaatc ttgaacgaga tagaattttt gattacctaa tgtagctgtt
     4021 ttagatacag caaatgtttt ttgaacttca ttagttgcct taccagtttt gtcgtaaaca
     4081 gtagtatata aaccactatt tgttggtttg atttgtgcga caccattatt agcatcaact
     4141 gttaatttac cagttgatgg tgttgatggt ttcgacggtg ttgttggttt tgatggtgta
     4201 gtaggggtag ttgtagattg cgtaccccat ggcgccactt tacccatttt tattaaatat
     4261 ttttcattaa ttaagtcata taattgatca taactataat tatgacttct taaatatcca
     4321 tgtggatcgg catggtccgt accacctaaa tatttactta cagcgtagtg agtccatact
     4381 gtaccatttc catcatactc agcactgtct ggttttaaac cataatattg taattgtgta
     4441 gctgcatagt cagcatagtt attcattgaa cgtgcaaatg aagcataatc gtgtgtgtgt
     4501 acgatttcaa cattgatgaa tctagggtta ccgactgcac ctacacccca tgataagtaa
     4561 tccgttggtg ctgtttcaat tatacgatcc ccatcaacaa acgcatgaac gaaagcattt
     4621 tgataattgt ttttcatata actaatttcg ccatttatcg tcgaacgatc attagctgta
     4681 tcatgaacta cgattccttc aggacgacct acgccgttac ggtatgcgta tttagggaag
     4741 taagatgtat aatcttcttc aatcttaggt gcttttaaat tatttttacg aatgtaatcg
     4801 ttaattgatg agtttacttg cggtttatat tttggcaaac tcgtttttgg tgctgcagcg
     4861 actgatcttg gttgtgctga agcactaaaa gtagttactt ttggtgtagc ttcagtttta
     4921 gctttaggtg caacaggtgt tgcttttggt gctgcggctt tatattgcgt ttcaagagca
     4981 gcaggtttag cagctgattt aattaattct ggatttattt gattttctga attatcatct
     5041 tcatcatcaa ctaaactata tccagcactt gtaacattat tgttcgtttt agatgctgtt
     5101 gtgttagttg tttttgtaac aggtttcgca ttatttgatg tagttgattg agtctcacga
     5161 gtgtcaccat ttacttgtgt agtatcaacc ttttgattta cttgagcatt gcctgtttta
     5221 ttcgctgctt ttggttggac aatagcaggg tcttgatata cttgagtgcc agaaatgttt
     5281 tgcgttggat tttttacctc agcttttgct tgttcagtag ttgctttaac tttattacta
     5341 tctaaaacgt ttttattagt agtttgatct tgtgtcgtct cagctgcttg aacttgatgt
     5401 gcagtgactg ctgaacctac aagcgttaat gcaaccattg atggtagttt gtaattaaat
     5461 tttttcgcca ttctatttat tactcctaac atttattaat tattaatctc attatagtac
     5521 ctgttttata cacctgtgcg tatttaacca atttgtaata tactcttaat ttatattttc
     5581 actgtaaaaa aatgaaaaaa attttgaatc tcaatggtga cgtgacaaat caaatagaat
     5641 tgatgtcaat ttaagaattg gtttattaca aatgtttcta taatagatac aacttttttt
     5701 aattaagcga ggtcaacttt tttgtcattt caatatgctc gatgccttcc tcaagaaata
     5761 tattacctct cattttaaaa tttaaacttt catagaatgg aattgcatga cattgggcat
     5821 tcatggtagc tacgtaaaaa ccttcatcta tagctaatga ctctacagct tgcataagca
     5881 ttctacccat gccttgtccg cgatgtgatt tcatcacagc tactcgttct attttgacag
     5941 ttgtttcatt aatagggcgt attcgagcag tggcaactgg ctgcccattg tcatacccaa
     6001 tcaggtgaat agattcagat tcatattcat caatttcact ttcctctggg acgccttgtt
     6061 cttctacaaa cactttcttt cttatataga agcaatcttc taacatcttt tgattgttta
     6121 cttttaaaaa cataaaatcc ctccatatcc caacaaaatc atttatattt aatttactaa
     6181 taataacata gaataacgat tgttattttt cacaaaaata attagacata aaaaatgcta
     6241 taaaaatgat tcataggaac catctttata gctagataga gtgtatttgt tataatgctt
     6301 cttttctaat aattgctgta tattgccaaa agattcgcat ttgagcaata ttccaattat
     6361 tcatccccat tgtaaaccca gaaggtttaa agatatttac atctgtcact tctaaagctg
     6421 ctgcgactaa atattgtaat ggaatactaa gtgatttcat tgtttcagta atgccatttt
     6481 catcatatac ccatgaaaat ggtaactctg attcgaaaac atcaacacta ttgaaaattt
     6541 caggtagggc taaaatataa catgcaccat ctacaatagg attctcgata ctatttttgt
     6601 aataaattag gagtgcttca tagttttctc gatgctcatg attgacgtaa aaaaattcac
     6661 ttctaaaatg agccatatct tggctatgaa taatttgttg ttctaacata ttaaacatcc
     6721 catttatatt tgcaatcttt tcgtatgttt ttctactcat gtatgatgct cctttcataa
     6781 atagtaaatg ttaatcgtca tataatatta gtttacaaca ccattttggt tatttgaagc
     6841 ttgtggcgtt tgttgagtgc caccttgatt ttgatttaag ttttgatctg tagcaggttg
     6901 ttgttgatta ttgacgctat tgttgctatt actgttatta ctgtcgtcgc tattattcga
     6961 agatttatct ttatcagtcg tatcgttttg ttcttttctc aataaactac tatctaaagg
     7021 cgttaatggt attaatgaac cataatgatt gatgacacgt tgatctaaga aatcattttt
     7081 atcattaata ggtgataatt ctaagtcttt acgaagtaag tttgcatatt tttgaatgct
     7141 gtctacactt ggatgataat agtaaatacc atttaacata tcatctttac cttttaattg
     7201 cgcagtttta atttcaacat catttgttaa gtacattttt gctaaagctt taatttcaga
     7261 gttagttaaa ttatgctttg catttttacc tacaatttga atcacgttat caagtttatc
     7321 aatagaatca acttcctgtg ctttttggaa taaaatctta attaattcca tttgacgttg
     7381 tccacgtttc aagtctgaat catgatgtct agttctagca actgctaaag cctcatcacc
     7441 atttaatttt tggtaccctt ttttaatttt aatcttacca gtatcatctg tatttggctc
     7501 atttaaatca tatggtacat catagtatat gccaccaagt tcatttacag cttctacaaa
     7561 ggccttcata ttaacgcgta catagtaatc aactggaaca ttcatcgttg cttcaacaga
     7621 gtccattgct gcaattggac caccatatgc atgtgcatgg gtaattttat cgtagtagcc
     7681 gactttagga atgtagctga ttgtatcacg aggaatacta agcattcgaa tttggtgttt
     7741 agattgatta aaagtagtta aaatcatagc gtctgatcta gagtgttcag catcttggcc
     7801 tttttttctt cttccgtcat tatcatcgat acctaagaag agaatagaga taggttgttc
     7861 ttcaggatta actttattgt ctcgtaaatt tgattgacga ttagcatttt tactatcttg
     7921 agaagattca aatgcatctt gagacgtttt aaaaagtaac gtagcgaaga caattggaac
     7981 aacaataaga accaatgcta gaaggatcaa aaagtatttt aaaaatttat tcatgattga
     8041 tgctcctata ttaaattttt gttaaatcat aatcataatt ttaaaatata attagttgaa
     8101 cgatatttat acaacttcat tactaataca ttatagcctt aaattgtagt actttatagt
     8161 aaaaaataca aatgttatgt caaagttcct tgtaatttga atgttttgtt aaaatcaaaa
     8221 agcattttaa aaaatataat gcgactttag tatgatgtct aaaaaaaatt aaaggggtaa
     8281 tataaatttg agtgatttat acaatgtgat aaggtataat taaatttgtt gtaataagaa
     8341 cttatatgaa gaaaatatta ctaagtagca taacgcataa gtgggtgaca gataaaattg
     8401 taagtgatat tataacgaag ttaaggttat tgttttaatt atttaatttg aatacaatga
     8461 gaggctatta gcgaatgaaa tttaataaag tgaaattggt tatacatgca tgcgtgcttt
     8521 tgttcatcat tatctctata ggattagttt ttcatcgatt acaaacgaaa acgaactcta
     8581 tagaacctat acataaggaa acaaaattat cggataatgc gaaatatttg gttgatcgta
     8641 ataaaggcaa aggtgagcca tcgaaattaa aggaagtata caatagtaaa gatccaaagt
     8701 ataagaagat tgaccagtat ttacaaaatt cattatttaa tggttcagta gcggtatatg
     8761 aaaatggcaa attgaaaatg agtaaaggct atggatatca agattttgaa aaaggaatta
     8821 aaaatacacc aaatacgatg tttctaattg gttcagctca aaaattttca acaggattac
     8881 tgttaaaaca gttagaagaa gaacataaaa ttaatattaa tgatccagta agcaaatata
     8941 ttccatggtt taaaacatct aagcccattc ctttaaaaga cttgatgctg catcaaagtg
     9001 gattatataa atataaatct tcaaaagact ataagaactt agatcaagca gtgagagcaa
     9061 ttcaaaaacg tggcattgat cctaagaaat acaaaaagca tatgtataat gatgggaatt
     9121 atttggtgct agctaaagtt atcgaagaag ttaccggcaa atcatatgcc gagaattact
     9181 atacaaaaat aggggatcct ctaaaacttc agcattcagc attttatgat gaaaaatcat
     9241 ttagaaaata tttagcaaaa ggttattctt ataatagtac aggactttct ttcttgaagc
     9301 ctaatgtttt agaacaatat tatggtgcag gtaatatata tatgacacca acagatatgg
     9361 gtaaattaat tactcaaatg caacaatata aattatttag tcctaaaata accaacccat
     9421 tattacatga gtttggtacg aaacagtatc cagatgaata tcgatatggt ttctatgtta
     9481 agccaacatt gaatagactt aacgggggat tctttggaca agtctttact gtttactata
     9541 atgataagta tgtagttgta cttgcattaa atgtaaaagg aaacaatgaa gttcgaatca
     9601 aacatattta taatgatatt ttaaaacaaa ataaacctta caatacgaag ggtgttattg
     9661 ttcaataatt aatatagaag atataacttg tatatggcat taaggcattg accttatttg
     9721 actagtatac gagttatatc ttctttttta tagaggtgaa aagtttaaag tataaggttg
     9781 aagtagtagg atgataaatt tgcgttaagt taaaaagata taaacaattg atgtaaatgt
     9841 caatataaaa atgtatataa atgattgtat aagaatgtat taattgaatg gataataatg
     9901 attgtctttt aagatataga aggggactta accatttaaa aattgaagga aaataaaaac
     9961 cagctaacaa aagtgttagc tggttaaact ttacattatt aaaatgttgt ttataagtgt
    10021 gaagagtgac cgccttgcat aacccaatat gttccgacaa cgaaacaaag tgtaattaca
    10081 agagcaaaga taactttgaa tgtttgtaaa cgtccatctt taccttcagt taaatgcatg
    10141 aacattaata attgaagtcc agcttggatg aatgcaaagc caaagataat tgtcaacttc
    10201 gcgtggaatg ttaatgacgt gtatagtgtt acgtaaactg ctaaaagcgt taatacgata
    10261 gatgcgataa atcctacagt atgtttcatt attgtactca tccgctatac accatcccta
    10321 tcatatatac ggcagtaaag atgaaaaccc aaacaacatc taagaagtgc cagtataaac
    10381 ttactataaa taattttggc gcattatatt tgtctaatcc gcgtcgttgg atttggatta
    10441 ataaacaaat ggcccaaacg atacctagcg atacgtgaca accatgcgtt cctaatagga
    10501 taaagaaact agaccagtaa gaaccaattg ttgggttaac gccttctgat gcatagtgtg
    10561 cgaattcata aatttcgaat ccaacaaaga ctaaacctaa aagtaacgta atgatcatcc
    10621 aaaacatcat taacttttgt ttttcttggc gcatgtagta aatagcaata ccacatgtgt
    10681 aagaactgaa taataatgca aacgtcatta ttaaaacaag aggcaattca aataactcag
    10741 tagtcatttt acctgcataa tcgccaccat gttgcaaagt taatagtgtc gcaaataggg
    10801 taccgaataa cgcaaattcg gctgtaatga aaatccaaaa gccaagctta tttaattcgc
    10861 cttcatgtgt gcgtgaatca atagtgtttg tatcatgact catgacttac agcctccctt
    10921 tctttaatac gcgcttctct taatctagct tcagtttcag caacttcagc agcagggata
    10981 tgatatccgt gatcgatttg gaaactgcga taaatcatag taccaaaaat accgaataaa
    11041 caaattaatg ctggaattac agtttcgaaa attaagaaga aaccgccgat agtcataaag
    11101 ataccaatcc agaatccaac aggagtattg tttggcatat gaatgtcttt gtaattatgg
    11161 ttgtctaagt aatgacgacc atgttctttc atatcaacaa atgtgtcgta gtcattccaa
    11221 tctggtgtaa tggcaaagtt gtatttaggt ggaattgctg atgcagtagt ccactctaga
    11281 gtacgaccaa ggccatccca gttatctcca gttgcttcac gtggagattt gaagtgactg
    11341 tatacgatac taacaacaag gaataagaat ccgattgcca ttaataatgc accgatagtt
    11401 gaaattaagt ttaataagaa ccaaccatct gatggcatat aagtgtataa acgacgtggc
    11461 ataccatcta atccaagaat gaattgtggt aagaaacaaa cgttaaatcc gatcatgaag
    11521 aaccagaagc accatttgtt taatgtttcg tttaatttgt aacccatcat ctttggatac
    11581 cagaagatta aaccagctaa gcaggcaaat acaacaccag taaccaatgt atagtggaag
    11641 tgagctacta agaagtacgt attgtgatat tgatagtcag ctgatgccat tgctaacatt
    11701 acacccgtaa caccacctaa aaggaagtta gggataaatg ctaatgagaa tagcattggt
    11761 gactcaaatg taatacgtcc tttatataat gttaataacc agttaaacaa tttcacacca
    11821 gttggaatac caatcagcat tgttgaaatt gagaagaatg agttgattaa cgcaccatta
    11881 cccattgtga agaaatggtg aacccaaact aagaaactaa ggaacgcgat accggcagtt
    11941 gcccatacca tactttgatg tccgaataaa cgcttacgag cgaatgtcgg gataatttct
    12001 gagtaaatac caaatgctgg aaggataacg atataaactt cagggtgccc ccatacccag
    12061 aagaagttag cccaaagcat tggcataccg ccatgtgcaa ctgtgaagaa tgctgtgtca
    12121 aatattctat cagttgtcat taatgctaac gctactgtta aaggagggaa agcaagaata
    12181 acaattaatg tagtaataaa tgttgttact gtaaacattg gcatttgcat aaacttcata
    12241 gttggtgttt tacatcttaa aattgttaca aagaagttga tacctgtagc taaggtacca
    12301 agccctgaaa tttgtatagc tattaagtaa tagttaacac ccggaccagg actgaattca
    12361 cctgctagtg gcgcatagtt tgtccaacca gctgctggtg aaccaccaat aataaatgac
    12421 aggttgaata aaatcatacc tgcaaagaat agccagaaac ttacgttgtt taatactggg
    12481 aatgcaacat cacgtgctcc aatttgtaat ggaacaacga tattccataa accaaagata
    12541 aatggcattg ccatgaagat aatcatgatt acaccatgtg tactaaaaat ttcgttatag
    12601 tggttagatt ctaaaaattt gttatcaggt actgttaatt gcgcacgaat aagtaacgca
    12661 tcaataccac cacggacgaa cattaatacg gcacagatta aatacataat accgattttc
    12721 ttatggtcta cagatgtgaa ccattctttg taaagatatt tccataattt aaagtaagta
    12781 attactgcga ttaaaccaat aactaagaat ggggcaccaa tttgtgccat tgtaatcatc
    12841 cagttacctt taactagtaa ttgatcccat ggaaaattca ttaatgtcca cctccatgat
    12901 catcattgtc ttgatcttgc gcatcttttg aaattttctt catttctttc gcatttttcg
    12961 attcatcttt cttgaactca ttgttatatg gttcgtcatt tccaagaatc atcaatttca
    13021 taccatgtcg tttatagttc gcatttgtaa tttgagcttt acgagcaggt attaatggtt
    13081 tgtctgatac atctttaaac atattttctt cactagtgaa gtttggatct ttcaattcga
    13141 aattgaaacg tttatatgca tagaagatgt attctggatc ggctgctgga tcaacaaacg
    13201 ccatatgtgt accattaaat tctaaagctt tattaggtgt gcttggtaat aattgtttat
    13261 caaatgtatc ttgatctaac gttttcttac ctttaacttc tttcacccat ttgtcgtagt
    13321 ctttttgact aacggcattt actttaaatg tttgacgtga gaatccttca ccattgaagt
    13381 tagagttacg acctctgaac gtaccagttt gagatgcttc taacgtccaa ttcattgtca
    13441 tgccagtcat ggcatatttt tgaccaccta attgtggaat ccagaaactt gtcattgtat
    13501 ccatagcttg aagcttaaat acaacaggac gatctttagg gattgttaat gtattaacag
    13561 tctctatatg ttcatctgga taagcaaaga accatttgta tcctgcactt actgcatata
    13621 caaccattgg atctttctca ctcttcggtg gtttttcgta atcgtataaa gttttaactg
    13681 taggaatagc taaagcagca acgattatga taggtattac aaaccatatt gtttcaatga
    13741 tggcattatg gtgcatctta ccagattcgg cattcttatt ataactatac ttgtaaataa
    13801 aaatggcgaa catgccaagt acaacgaaac aaataacaag catgaagacg attgaataaa
    13861 gaatcaagaa cttctgacta cttgctactg gcccttttgc gttgaaaatt tctatatttg
    13921 aacaaccact aagtaaaatt agtgtgccaa ataatagaag caaagactta aattttgaca
    13981 cttttttgac ctcctaatac tacaaatgta gggcttaaca ttaattttaa gttattacac
    14041 aatatttaca agggcttatg ggaaaaaaat taataaaatt gtatcaaaaa tgttgataaa
    14101 tcaaggtgtg acgtgggttc acacatttgt taaaattatg tgtacatttt gtgactaata
    14161 gcgtttttga aaaaattgct tcatatttaa aagggaagtt gagaatagct ttcaattaaa
    14221 ggttaattga atgaaaataa aaaagtttga aattgtctaa taa
//
LOCUS       DAHHOJ010000072        14046 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.072, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000072 DAHHOJ010000000
VERSION     DAHHOJ010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 14046)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14046)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14046
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.072"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(124..297)
                     /locus_tag="NBH32_001782"
     CDS             complement(124..297)
                     /locus_tag="NBH32_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NETI motif-containing protein"
                     /protein_id="HCD1515375.1"
                     /translation="MKFKVNENETIADCLSRMKMQGYMPVKRIEKPVFQEQNDGTVEV
                     SHQEIIFVGKKIQ"
     gene            complement(278..880)
                     /locus_tag="NBH32_001783"
     CDS             complement(278..880)
                     /locus_tag="NBH32_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HCD1515376.1"
                     /translation="MSFVTENPWLMVLTIFIINVCYVTFLTMRTILTLKGYRYIAASV
                     SFLEVLVYIVGLGLVMSNLDHIQNIIAYAFGFSIGIIVGMKIEEKLALGYTVVNVTSA
                     EYELDLPNELRNLGYGVTHYAAFGRDGSRMVMQILTPRKYERKLMDTIKNLDPKAFII
                     AYEPRNIHGGFWTKGIRRRKLKDYEPEELESVVEHEIQSK"
     gene            complement(1151..1972)
                     /gene="nadE"
                     /locus_tag="NBH32_001784"
     CDS             complement(1151..1972)
                     /gene="nadE"
                     /locus_tag="NBH32_001784"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCD1515377.1"
                     /translation="MSKLQDVIVQEMKVKKRIDSAEEIMELKQFIKNYVQSHSFIKSL
                     VLGISGGQDSTLVGKLVQMSVNELREEGIDCTFIAVKLPYGVQKDADEVEQALRFIEP
                     DEIVTVNIKPAVDQSVESLKEAGIVLTDFQKGNEKARERMKVQFSIASNRQGIVVGTD
                     HSAENITGFYTKYGDGAADIAPIFGLNKRQGRQLLAYLGAPKELYEKTPTADLEDDKP
                     QLPDEDALGVTYEAIDNYLEGKPVTPEEQKVIENHYIRNAHKRELAYTRYTWPKS"
     gene            complement(1965..3434)
                     /locus_tag="NBH32_001785"
     CDS             complement(1965..3434)
                     /locus_tag="NBH32_001785"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCD1515378.1"
                     /translation="MYQLEDDSLMLHNDLYQINMAESYWNDNIHEKMAVFDLYFRKMP
                     FNSGYAVFNGLKRVIDFIEHFGFSESDLEYLKSIGYKDDFLSYLKDLKFTGSIRSMQE
                     GELCFGNEPLLRVEAPLIQAQLIETILLNIVNFHTLITTKASRIRQIASNDKLMEFGT
                     RRAQEIDAALWGARAAYIGGFDSTSNVRAGKLFGIPVSGTHAHAFVQTYGDEYVAFKK
                     YAERHKNCVFLVDTFHTLKSGVPNAIKVAKELGDKINFVGIRLDSGDIAYLSKEARRM
                     LDEAGFTETKIIASNDLDEETITSLKAQGAKVDSWGVGTKLITGYDQPALGAVYKLVA
                     IENEDGSYSDRIKLSNNAEKVTTPGKKNVYRIINKKTGKAEGDYITLENENPYDEQPL
                     KLFHPVHTYKMKFIKSFEAIDLHHNIYENGKLVYQMPTEDESREYLAQGLQSIWDENK
                     RFLNPQEYPVDLSKACWDNKHKRIFEVAEHVKEMEEDNE"
     gene            3618..4694
                     /locus_tag="NBH32_001786"
     CDS             3618..4694
                     /locus_tag="NBH32_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide synthase oxygenase"
                     /protein_id="HCD1515379.1"
                     /translation="MLFKEAQAFIENMYKECHYETQIINKRLHDIELEIKETGTYTHT
                     EEELIYGAKMAWRNSNRCIGRLFWDSLNVIDARDVTDEASFLSSITYHITQATNEGKL
                     KPYITIYAPKDGPKIFNNQLIRYAGYDNCGDPAEKEVTRLANHLGWKGKGTNFDVLPL
                     IYQLPNESVKFYEYPTSLIKEVPIEHNHYPKLRKLNLKWYAVPIISNMDLKIGGIVYP
                     TAPFNGWYMVTEIGVRNFIDDYRYNLLEKVADAFEFDTLKNNSFNKDRALVELNYAVY
                     HSFKKEGVSIVDHLTAAKQFELFERNEAQQGRQVTGKWSWLAPPLSPTLTSNYHHGYD
                     NTVKDPNFFYKKKESNANQCPFHH"
     gene            4714..5508
                     /locus_tag="NBH32_001787"
     CDS             4714..5508
                     /locus_tag="NBH32_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HCD1515380.1"
                     /translation="MQLYYLGPKGTFSYLACRQYFSENEATFQPKSNLFEVIKAVADD
                     DTSIGVVPIENSIEGTINIVADALAQQDVFAHGEIRLDINFALYGNGSNTISDIKKVY
                     SIAPAISQTTNYIHQHQFDYDYVDSTIQSLTKIENGVAAIAPLGSGEAYGFTPIDTHI
                     EDYPHNVTRFLVIKNQQQFDQNATSLMFLITPMHDKPGLLASVLNTFALFNINLSWIE
                     SRPLKTQLGMYRFFVQADSAITTDIKKVIAILETLDFKVEMIGAFN"
     gene            5580..5687
                     /locus_tag="NBH32_001788"
     CDS             5580..5687
                     /locus_tag="NBH32_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515381.1"
                     /translation="MVDFLLFVLFLHDAAVYVSLPLVQAIANVTFYFSF"
     gene            complement(5698..7260)
                     /locus_tag="NBH32_001789"
     CDS             complement(5698..7260)
                     /locus_tag="NBH32_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCD1515382.1"
                     /translation="MAYFNQHQSMISKRYLTFFSKSKKKKPFSAGQLIGLILGPLLFL
                     LTLLFFHPQDLPWKGVYVLAITLWIATWWITEAIPIAATSLLPIVLLPLGHILTPEQV
                     SSEYGNDIIFLFLGGFILAIAMERWNLHTRVALTIINLIGASTSKILLGFMVATGFLS
                     MFVSNTAAVMIMIPIGLAIIKEAHDLQEANTNQTSIQKFEKSLVLAIGYAGTIGGLGT
                     LIGTPPLIILKGQYMQHFGHEISFAKWMIVGIPTVIVLLGITWLYLRYVAFRHDLKYL
                     PGGQTLIKQKLDELGNMKYEEKVVQTIFVLASLLWITREFLLKKWEVTSSVADGTIAI
                     FISILLFIIPAKNTEKHRRIIDWEVAKELPWGVLILFGGGLALAKGISESGLAKWLGE
                     QLKSLNGVSPILIVIVITIFVLFLTEVTSNTATATMILPILATLSVAVGVHPLLLMAP
                     AAMAANCAYMLPVGTPPNAIIFGSGKISIKQMASVGFWVNLISAIIIILVVYYVMPIV
                     LGIDINQPLPLK"
     gene            complement(7465..8562)
                     /locus_tag="NBH32_001790"
     CDS             complement(7465..8562)
                     /locus_tag="NBH32_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectate lyase"
                     /protein_id="HCD1515383.1"
                     /translation="MYINMKDYGLTGINKTKDTRAIQRALNRGRCKPTTVYIPKGTYD
                     ICKPLTIYGNTTLLLDNETILRRCHSGPLLKNGHRFGFYRGYNGHSHIHIKGGKFDMN
                     GVSYPYNNTAMCIGHAEDIQLIGVTIKNVVSGHAIDACGINGLYIKSCSFEGFIDYSG
                     ERFYSEAIQLDIQVPGAFPKFGTTDGTITKNVVIEDCYFGPSELPEMGSWNRAIGSHA
                     SRHNRYYENIHIRNNIFEDIQGYALTPLKYKDAFIINNKFINCEGGIRYLGVRDGKNA
                     ADVMTGKDLGSQAGINMNIIGNEFKGSMSKDAIHVHNYNNVKHKDVLIVGNTFNDSTQ
                     SIHLEDIDTVFLSPVEAGIQVTTINIDEIKK"
     gene            8934..9494
                     /locus_tag="NBH32_001791"
     CDS             8934..9494
                     /locus_tag="NBH32_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HCD1515384.1"
                     /translation="MTHRALLVVDYSYDFIADDGLLTCGKPGQNIEDFIVSRINDFNY
                     YQDHIFFLMDLHYLHDIHHPESKLFPPHNIVDTSGRELYGKVGKLYETIKAQPNVHFI
                     DKTRYDSFFGTPLDSLLRERSINQVEIVGVCTDICVLHTAISAYNLGYKISVPAEGVA
                     SFNQKGHEWALAHFKNSLGAEVEQHV"
     gene            9547..10476
                     /locus_tag="NBH32_001792"
     CDS             9547..10476
                     /locus_tag="NBH32_001792"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HCD1515385.1"
                     /translation="MAKTYIFGHKNPDTDAISSAIIMAEFEQLRGNSGAKAYRLGDVS
                     AETQFALDTFNVPAPELLTDDLDGQDVILVDHNEFQQSSDTIASATIKHVIDHHRIAN
                     FETAGPLCYRAEPVGCTATILYKMFRERGFEIKPEIAGLMLSAIISDSLLFKSPTCTQ
                     QDVKAAEELKDIAKVDIQKYGLDMLKAGASTTDKSVEFLLNMDAKSFTMGDYVTRIAQ
                     VNAVDLDEVLNRKEDLEKEMLAVSAQEKYDLFVLVVTDIINSDSKILVVGAEKDKVGE
                     AFNVQLEDDMAFLSGVVSRKKQIVPQITEALTK"
     gene            complement(10671..10844)
                     /locus_tag="NBH32_001793"
     CDS             complement(10671..10844)
                     /locus_tag="NBH32_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515386.1"
                     /translation="MNVVAVNFKWSFELYMLKVENRAMNRVHNNIYNFNHNEKGRKKT
                     KIYTQHRKYFKKM"
     gene            10896..12275
                     /locus_tag="NBH32_001794"
     CDS             10896..12275
                     /locus_tag="NBH32_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCD1515387.1"
                     /translation="MNIIEQKFYDSKAFFNTQQTKDISFRKEQLKKLSKAIKSYESDI
                     LEALYTDLGKNKVEAYATEIGITLKSIKNARKELKNWTKTKNVDTPLYLFPTKSYIKK
                     EPYGTVLIIAPFNYPFQLVFEPLIGAIAAGNTAIIKPSELTPNVARVIKRLINETFDA
                     NYIEVIEGGIEETQTLIHLPFDYVFFTGSENVGKIVYQAASENLVPVTLEMGGKSPVI
                     VDETANIKVASERICFGKFTNAGQTCVAPDYILVHESVKDDLITALSKTLREFYGQNI
                     QQSPDYGRIVNLKHYHRLTSLLNSEQMNIVFGGHSDEDERYIEPTLLDHVTSDSAIMQ
                     EEIFGPILPILTYQSLDEAIAFIHQRPKPLSLYLFSEDENATQRVINELSFGGGAIND
                     TLMHLANPKLPFGGVGASGMGRYHGKYSFDTFTHEKSYIFKSTRLESGVHLPPYKGKF
                     KYIKAFFKN"
     gene            complement(12395..13423)
                     /locus_tag="NBH32_001795"
     CDS             complement(12395..13423)
                     /locus_tag="NBH32_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HCD1515388.1"
                     /translation="MTNGYIGSYTKKNGKGIYRFELNENQSRIDLLETGFELEASTYL
                     VRNNEVLYGINKEGEQCGVASLKIDDNGELHLLNKCLSSKAGTGCYVSISEDKRYLFE
                     AVYGAGIIRMYELNTHTGEIIRLIQELAHDFPTGTHERQDHPHAHYINQTPDGKYVAV
                     TDLGADRIVTYKFDDNGFEFYKESLFKDSDGTRHIEFHDNGKFAYVVHELSNTVSVAE
                     YNDGKFEELERHLTIPENFDGDTKLAAVRLSHDQQFLYVSNRGHDSIAIFKVLDNGQH
                     LELVTITESGGQFPRDFNIASSDDLLVCAHEQGDSVVTVFERNKETGKITLCDNTRVA
                     SEGVCVIF"
     gene            complement(13882..>14046)
                     /locus_tag="NBH32_001796"
     CDS             complement(13882..>14046)
                     /locus_tag="NBH32_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaI/MecI/CopY family transcriptional regulator"
                     /protein_id="HCD1515389.1"
                     /translation="IKEDDIKMKTAKTFLNKLYGGDMKSLVLNFAKNEELNNKEIEEL
                     RDILNDISKK"
BASE COUNT         4662 a         2511 c         2034 g         4839 t
ORIGIN      
        1 ctttctcata caatatgaat ctagcatctt gcatgtataa acaaataggc tatccaaagt
       61 aaaattactc aaactttgaa tagcctttcc tattcaaata gtctttattt aaattggatt
      121 atgttattgg attttcttac ctacaaaaat gatttcttga tgtgatactt caaccgtgcc
      181 atcattttgc tcctgaaaca cgggtttttc aatacgtttt accggcatat acccttgcat
      241 tttcattcgt gacaaacaat cagcaattgt ttcgttctca tttactttga atttcatgtt
      301 ctactacact ttccagttct tctggttcat aatctttaag ctttctacga cgaatgcctt
      361 tagtccagaa tccaccatgt atgtttcgag gttcatacgc aatgataaat gctttcggat
      421 ctaaattttt tatcgtatcc atcaatttac gttcatattt tcttggtgtt aaaatttgca
      481 tcaccatacg actaccatct ctaccaaacg cagcatagtg cgtaacgcca tatcctaaat
      541 ttcgaagttc attcggcaaa tctaactcat attctgctga agttacattt acaactgtat
      601 aacctaatgc cagtttttct tctattttca taccaacaat gatacctatt gaaaaaccaa
      661 atgcgtaggc aataatattt tgaatatggt ctaaattaga cataaccaaa cctaaaccaa
      721 cgatataaac taatacttct aaaaaactaa ctgatgcagc aatataacga taacctttca
      781 acgttaaaat tgttcgcatc gttaaaaacg ttacataaca aacgttaatg ataaatatag
      841 ttagtaccat taaccatgga ttttctgtta cgaatgacat aaggcgcccc actctctttc
      901 aattacactt taaataccaa actgcttcaa actaacctta atgaatgcat attaatataa
      961 tatagtacga aaactctcat aaataaatag cgaatttgtc ataaataaaa atttattctc
     1021 aaatgaacat acttacttta gttcatacac tttcaattta tacactgaag tagtctagct
     1081 gaatgtggtt taatgttatt aattctaact catatttaat ttaagtcaca cgttagaaga
     1141 aaaaattaaa ttaggatttt ggccacgtgt atcttgtata tgcaagttca cgtttgtgtg
     1201 catttcgtat ataatgattt tcaattactt tttgttcttc tggcgtaact ggcttacctt
     1261 ctaaataatt atcaatcgcc tcataagtta cacctaatgc atcttcatct ggaagctgtg
     1321 gtttatcatc ttctaaatca gcagttggcg ttttttcata taattccttt ggcgcaccaa
     1381 gatacgctaa taattgacga ccttgtcgtt tattcaaacc aaatataggt gcgatatctg
     1441 cagcaccatc accgtacttc gtataaaaac cagttatatt ttcagctgaa tgatctgttc
     1501 ctactacaat accttgtcgg tttgaagcaa ttgaaaattg tactttcata cgttcacgcg
     1561 ctttttcatt tcctttttgg aaatctgtaa gaacaatacc ggcttctttt aatgattcca
     1621 cactttgatc aactgcaggt ttaatattta cagttactat ttcgtctggt tcaatgaatc
     1681 gcaaagcttg ctcaacttca tcagcatctt tttgaactcc ataaggtaat ttaactgcaa
     1741 taaacgtaca atcaatgcct tcttcacgta attcgttaac agacatttgt actaattttc
     1801 caactaatgt agaatcttgt cctcctgaaa tacctaacac taaagatttt ataaatgaat
     1861 gtgattgtac ataatttttt ataaattgct ttaattccat aatttcttca gcactatcga
     1921 tacgcttttt cactttcatt tcttgtacaa taacgtcttg taatttactc attatcttct
     1981 tccatctcct taacgtgttc cgcaacttca aaaatacgtt tatgtttatt atcccaacat
     2041 gccttgctta aatcgactgg atattcttgt ggattcagga aacgcttatt ttcatcccaa
     2101 atagattgta atccttgtgc taaatattca cgtgattcat cttctgttgg catttgatat
     2161 actaatttac cattttcata aatattatga tgcaaatcaa tggcttcgaa agattttata
     2221 aatttcattt tataagtatg cactggatgg aataatttta aaggttgttc atcgtatgga
     2281 ttttcatttt ccaaagtaat ataatcgcct tctgccttac ctgttttctt gtttataatg
     2341 cgatatacat ttttcttacc tggcgtcgta accttttcag cgttatttga taatttaata
     2401 cgatcactat atgaaccatc ttcattttca atagctacaa gtttatatac tgcacctaat
     2461 gctggttgat catatcctgt aatcagcttt gtaccaacgc cccaagaatc tacttttgca
     2521 ccttgtgctt tcaaactcgt aatcgtttct tcatccaaat cattagacgc gataatttta
     2581 gtttcagtaa atcctgcttc atcaagcata cgtcttgcct ctttagataa ataagcgata
     2641 tctccagaat ctaatcgaat acctacaaag ttaattttgt cacctaattc ttttgcaact
     2701 tttattgcat ttggcacgcc agattttaaa gtatggaatg tatctactag gaacacacaa
     2761 tttttatgtc tttcagcata ttttttgaag gcaacatatt cgtctccata agtttggaca
     2821 aatgcatgtg catgtgtacc agacacaggt ataccaaata atttacccgc cctaacatta
     2881 cttgtagaat caaagccccc gatgtaagca gctctagcgc cccacaatgc tgcatcaatt
     2941 tcttgcgcac gacgtgtacc aaactccatt aatttatcat ttgatgcaat ttgacgaatt
     3001 ctactagcct ttgttgtaat tagtgtatgg aaatttacaa tgtttaataa aattgtttct
     3061 attaattgcg cttgaatcaa tggtgcttct acgcgtaaca atggttcgtt accaaagcat
     3121 aattcgcctt cttgcatcga acggatgcta cctgtgaatt ttaaatcttt taaatatgat
     3181 aagaaatcat ccttgtagcc aatagacttt aaatattcca aatcagattc tgaaaatcca
     3241 aaatgttcta taaaatcaat gacgcgtttt aaaccattaa aaacagcata gccactatta
     3301 aatggcattt ttctaaaata caaatcaaat acagccattt tttcatgaat attatcattc
     3361 caataacttt cagccatatt tatttgatat aagtcattat gtaacattaa actgtcgtct
     3421 tctaattggt acactcgtat ctctccaatc gacctaaata ttttcttaca ttttatcata
     3481 attcattttt ttatatacat aagagcccct taatttccat acttttaatt aaaatcaacc
     3541 aacaatttaa tgacatatac ataattttta agagtatttt aataatgtag actataatat
     3601 aaagcgaggt gttgttaatg ttatttaaag aggctcaagc tttcatagaa aacatgtata
     3661 aagagtgtca ttatgaaacg caaattatca ataaacgttt acatgacatt gaactagaaa
     3721 taaaagaaac tgggacatat acacatacag aagaagaact tatttatggt gctaaaatgg
     3781 cttggcgtaa ttcaaatcgt tgcattggtc gtttattttg ggattcgtta aatgtcattg
     3841 atgcaagaga tgttactgac gaagcatcgt tcttatcatc aattacttat catattacac
     3901 aggctacaaa tgaaggtaaa ttaaagccgt atattactat atatgctcca aaggatggac
     3961 ctaaaatttt caacaatcaa ttaattcgct atgctggcta tgacaattgt ggtgatcctg
     4021 ctgaaaaaga agttacacgc ttagcaaatc acttaggttg gaaaggaaaa ggtactaatt
     4081 ttgacgtgtt accactaatt taccaattac ctaatgagtc agttaaattt tacgaatatc
     4141 ctacttcatt aattaaagaa gtacctattg aacataatca ttatccaaaa ttaagaaaat
     4201 tgaacttaaa atggtatgca gtccctatca tttccaatat ggacttaaaa atcggtggca
     4261 ttgtatatcc aactgccccc tttaacggtt ggtatatggt aactgaaatt ggcgtacgta
     4321 actttattga tgattaccgt tacaatttac tagaaaaagt tgcagatgcg tttgaatttg
     4381 atacacttaa aaataattca tttaataaag atcgagcact tgttgaattg aactatgctg
     4441 tgtatcattc ctttaaaaaa gaaggcgtat caattgtcga tcatttgacc gctgcaaagc
     4501 aattcgaact attcgaacgt aatgaagcac aacaaggtcg tcaagttacc ggaaaatggt
     4561 cttggctagc gccgccatta tctccaacat tgacgtcaaa ttatcatcac ggatatgaca
     4621 atacagtaaa agatccaaac tttttctata aaaagaaaga atcaaatgct aaccagtgcc
     4681 ctttccatca ttaagttagt agaggtgtag catatgcaat tatattattt aggccctaaa
     4741 ggtacatttt catatttggc ttgtcgtcag tatttttctg aaaatgaagc aacttttcaa
     4801 ccaaagtcaa atttatttga agtcatcaag gcagttgcag atgatgacac atcgattggt
     4861 gttgtcccta ttgaaaattc tatagaagga acaatcaaca ttgtggcaga tgcacttgca
     4921 cagcaagatg tctttgcaca cggtgaaata cgtctagata ttaactttgc attatatggt
     4981 aatggttcga acaccatttc tgatattaaa aaagtgtatt ctattgcacc agcaatcagt
     5041 caaacaacaa attacataca tcaacatcaa tttgactatg attatgtcga cagtacaatt
     5101 caaagtttga ctaaaattga aaatggcgtt gcagcaattg cacctttagg tagtggcgaa
     5161 gcttatggtt ttacacctat tgatacacat attgaagact atccacataa cgtgacacgt
     5221 tttttagtta taaaaaatca gcagcaattt gatcaaaatg cgacatcatt gatgttttta
     5281 attacaccaa tgcatgataa acctgggttg ttagcaagtg tgcttaatac atttgctttg
     5341 tttaatatta atttatcctg gattgagtct cgtcctttaa aaacacaatt aggtatgtat
     5401 cgatttttcg ttcaggctga ttctgcaatt acgacagata taaagaaagt cattgctatt
     5461 ttagaaacct tagatttcaa agtagaaatg attggtgctt ttaattaaac tatattttct
     5521 aggttctgca atttaaataa aataaagagt caaccaagga ttaaaataga aatccatcct
     5581 tggttgactt tcttttgttt gtattgtttt tacacgatgc tgcggtatac gtttcgttac
     5641 cactcgttca agcaattgct aatgtaacct tttatttttc gttctaatct gcaattacta
     5701 tttcaatggc agtggttgat ttatatcaat acctaaaact ataggcatta cataatacac
     5761 aactaaaata ataattattg cactgattaa gtttacccag aatcctactg atgccatttg
     5821 tttgatagat attttacctg aaccaaagat aattgcattc ggtggtgtcc ctactggtaa
     5881 catgtatgca cagttagccg ccatagctgc aggtgccata agtagtaatg gatgcacacc
     5941 aaccgcaaca gataacgttg ctaaaatcgg taaaatcatc gttgcagttg cggtattaga
     6001 tgtcacttca gttaaaaata ggacaaagat agttatgacg attacaataa gaatcggact
     6061 gacaccattt aatgatttca actgttcgcc taaccatttt gctaaaccac tttcagaaat
     6121 acctttcgct aatgctaaac cgccaccaaa taaaattaag acaccccaag ggagctcttt
     6181 tgcaacttcc cagtcaatga tacgtcgatg tttttcagta tttttagctg gaataataaa
     6241 taataatatt gatataaaaa tagcaatcgt accatctgca acggatgacg taacttccca
     6301 ttttttcaga agaaactctc ttgtaatcca taataagctg gcaagtacaa agatagtttg
     6361 tactaccttt tcttcatact tcatattgcc aagctcgtct agcttttgtt taattaacgt
     6421 ctgaccacca ggtaaatatt tcaaatcatg tctaaacgca acatatctta aatagagcca
     6481 agtaatacct aacaaaacaa tgaccgttgg aatccctaca atcatccatt tagcaaaact
     6541 aatttcatgt ccaaaatgtt gcatgtattg tccttttaaa ataattagcg gcggggttcc
     6601 gattaatgta cccaagccac caatcgtacc tgcatagcca attgctaaaa ctagagattt
     6661 ttcaaacttt tgaatacttg tttgattcgt attggcttct tgtaaatcat gtgcttcctt
     6721 aataattgct aaaccaatcg gaatcatgat cattacagct gcagtgttcg atacaaacat
     6781 agataagaat cctgttgcca ccatgaatcc aagtaatatt tttgaagtgc ttgcaccaat
     6841 taaattaata atagtcaaag ctactctcgt atgtaaattc catctttcca ttgcaattgc
     6901 caaaataaat ccacctaaaa acaaaaagat aatatcatta ccatattcgg atgatacttg
     6961 ttctggtgta agtatgtgac ctaatggtaa taacacaatt ggtaataagc tcgttgctgc
     7021 aataggaatt gcttcagtaa tccaccaagt cgcaatccat aaagtaatcg ctaagacata
     7081 tacgcctttc caaggtaagt cttgtggatg aaagaacaat aatgttaata ggaaaagtaa
     7141 tggacctaat attagtccaa taagttgtcc cgcactaaac ggtttctttt tctttgattt
     7201 tgaaaagaat gttaaatacc ttttcgatat cattgattga tgttgattga aataagccat
     7261 aaatttcccc ctcatgatga ttatgacaag ctacttcaac tcactatatt agagtataga
     7321 aagttgtcta tcttgtatta tcattaccgt caaaataccc cttttatcag ataattgggt
     7381 ttattatagc atatttatac cgttttatga atgcgctttc aaaaattatc tactattctt
     7441 aatcctaatg tcatgcgaaa ctttttactt ttttatttca tctatattga ttgtagtaac
     7501 ttgaataccc gcttcaacag gacttaaaaa cactgtatca atatcttcta aatgaattga
     7561 ttgagtcgaa tcattgaatg tattcccaac gattaataca tctttatgtt taacattatt
     7621 ataattatgt acatgtatcg catctttaga cattgatcct ttaaattcat ttccaatgat
     7681 attcatattt atgcctgctt gggaacctaa gtcttttcct gtcatcacat ctgcagcatt
     7741 tttaccatct ctaactccta aatatctaat accaccctca cagttaataa acttattatt
     7801 aataatgaaa gcatctttat acttcaaggg agttaatgca taaccttgta tatcttcaaa
     7861 tatattattt ctaatatgaa tattctcata gtaacgatta tgtctacttg catgtgagcc
     7921 aatagcacga ttccaacttc ccatttcggg caattctgaa ggtccaaaat aacaatcttc
     7981 gataacgaca tttttcgtta tcgtaccatc tgtcgttccg aattttggaa aagcaccagg
     8041 tacttgaatg tctaattgta ttgcttcaga ataaaagcgt tcgccactat agtctataaa
     8101 tccttcaaat gaacagcttt taatatagag tccattaatc ccgcaagcat caattgcatg
     8161 accactcact acattcttaa tagtcacacc tattaattga atatcttcag catgcccaat
     8221 gcacatagct gtattgttat aaggatacga tacgccattc atatcaaact tgccgccttt
     8281 aatatgaata tgactgtgtc cattataacc acgataaaaa ccaaagcgat gaccattttt
     8341 taataaagga ccagaatgac atcggcgtaa aatagtttca ttatccaaca aaagtgttgt
     8401 attgccatat atcgttaatg gtttgcaaat atcatacgtc cctttcggta tataaactgt
     8461 cgttggttta catcttccac gatttaacgc acgttgtatt gctcgagtat ctttagtttt
     8521 gtttatgcct gttaacccat aatctttcat attgatatac ataaatcgtc atcctttgtc
     8581 tattttctaa ttactcatat attaaccaat tattcaaatt ataatcgtgt taggttgatt
     8641 taacagatag tcataaatct cgtgatgcca tctattagca tttttatatt gaaaacatca
     8701 acacttgata ttagttctaa gtttgtcaca tttaatattc tattacagct attttaaagc
     8761 tatcgtaaaa acatcttata atatttgttg attgcgtgat ttgctcttcc aaaaaagttg
     8821 attaatctat atacttaatc tatatttata tattaaccat tagggttaaa aaatactcta
     8881 gcatttatga atagatggga gtttatttta ttattatata ggagagatgt tgaatgacac
     8941 atcgcgcact attagttgtt gactattcat atgactttat cgcagacgac ggcttactaa
     9001 catgcggtaa acctggacaa aatattgaag attttattgt ttctcgtatc aatgacttta
     9061 attattatca agaccatata ttctttttaa tggatttaca ttatttacat gacattcatc
     9121 atcctgaaag taaattattc ccaccacaca atatcgtaga tacaagtggt agagaattat
     9181 acggtaaagt aggtaaatta tacgaaacaa ttaaagcgca acctaatgta catttcattg
     9241 ataaaacgcg ctatgattcg ttctttggta ccccgcttga tagtttattg agagaaagaa
     9301 gtattaatca agtcgaaatc gttggtgtat gtaccgatat ttgcgtgtta catacagcaa
     9361 tttctgcata caacttaggt tataaaattt cagtacctgc tgagggagtg gcttcattta
     9421 atcaaaaagg gcatgaatgg gcacttgcac atttcaaaaa ctcattaggt gcagaggtag
     9481 aacaacacgt ttaaatcgtg ctaaaataat tataaagaat acaatataca agggagatat
     9541 ttgacaatgg ctaaaacata tattttcgga cataagaatc cagacactga tgcaatttca
     9601 tctgcgatta ttatggcaga atttgaacaa cttagaggta attcaggagc caaagcatac
     9661 cgtttaggtg atgtgagtgc agaaactcaa ttcgcgttag atacatttaa tgtacctgct
     9721 ccggaattat taacggatga tttagatggt caagatgtta tcttagttga tcataacgaa
     9781 ttccaacaaa gttctgatac gattgcctct gctacaatta agcatgtaat tgatcatcac
     9841 agaattgcaa atttcgaaac tgctggtcct ttatgttatc gtgctgaacc agttggttgt
     9901 acagctacaa ttttatacaa aatgtttaga gaacgtggct ttgaaattaa acctgaaatt
     9961 gccggtttaa tgttatcagc aattatctca gatagcttac ttttcaaatc accaacatgt
    10021 acacaacaag atgttaaagc agctgaagaa ttaaaagata ttgctaaagt tgatattcaa
    10081 aagtacggct tagatatgtt aaaagcaggt gcttcaacaa ctgataaatc agttgaattc
    10141 ttattaaaca tggatgctaa atcatttact atgggtgact atgtgactcg tattgcacaa
    10201 gttaatgctg ttgaccttga cgaagtgtta aatcgtaaag aagatttaga aaaagaaatg
    10261 ttagctgtaa gtgcacaaga aaaatatgac ttatttgtac ttgttgttac tgacatcatt
    10321 aatagtgatt ctaaaatttt agttgtaggt gctgaaaaag ataaagtcgg cgaagcattc
    10381 aatgttcaat tagaagatga catggccttc ttatctggtg ttgtttctcg aaaaaaacaa
    10441 atcgtacctc aaatcactga agcattaaca aaataatact atattactgt ctaatcatag
    10501 acatgttgta tttaactaac agttcattaa agtagaattt atttcacttt caatgaactg
    10561 ttttttattt acgtttgact aatttacaac cccttttcaa tagtagtttt cattccttta
    10621 gctaccctaa cccacagatt agtgatttct atacaattcc ccttttgtct ttacattttc
    10681 ttaaaatatt tgcgatgttg agtataaatt tttgttttct tcctaccttt ttcgttatga
    10741 ttaaagttat aaatattatt atgtacacga ttcatcgctc tattttcaac tttcaacata
    10801 tataattcga aagaccattt aaaattaacg gccacaacat tcaaatcaat taatcacttt
    10861 ttccaaaata atcatataag gaggttcttt tcattatgaa tattattgag caaaaatttt
    10921 atgacagtaa agcttttttc aatacacaac aaactaaaga tattagtttt agaaaagagc
    10981 aattaaagaa gttaagcaaa gctattaaat catacgagag cgatatttta gaagcactat
    11041 atacagattt aggaaaaaat aaagtcgaag cttatgctac tgaaattggc ataactttga
    11101 aaagtatcaa aaatgcccgt aaggaactta aaaactggac taaaacaaaa aatgtagaca
    11161 cacctttata tttatttcca acaaaaagct atatcaaaaa agaaccttat ggaacagttt
    11221 tgatcattgc accatttaac tatccttttc aactagtgtt cgaaccttta atcggtgcta
    11281 ttgcagcagg taatacggca attattaaac catctgagtt gacaccaaat gttgcacgag
    11341 tgattaaacg attaatcaat gaaacatttg atgcaaatta cattgaagtt attgagggag
    11401 gaattgaaga aacgcaaacg ttaattcact taccttttga ctatgtcttc tttacaggaa
    11461 gtgaaaatgt aggcaaaatc gtttatcaag ctgccagcga aaatttagtt cctgtgacat
    11521 tagaaatggg cggtaaatct ccagtcatcg ttgatgaaac agcgaatatt aaagttgcta
    11581 gtgagcgcat ttgttttggg aaattcacta atgccggcca aacatgtgtt gcaccagatt
    11641 acattttagt acacgaatct gtaaaagatg atttaatcac agccctatca aaaacgttgc
    11701 gtgaatttta tggtcaaaat atacaacaaa gtccagatta tggccgcatt gtaaacctta
    11761 aacattatca tcgtctgact tcattactta acagtgaaca aatgaatatt gtatttggtg
    11821 gtcatagtga tgaggatgaa cgttatatag aaccaacatt gttagatcac gttacaagtg
    11881 attcagcaat tatgcaagaa gaaatttttg gtcctatctt accgatttta acgtatcagt
    11941 cattggatga agcaatagcc tttattcacc aaagaccaaa acctttgagt ttatatttat
    12001 ttagcgaaga tgaaaatgct acacaacgtg taataaacga gctatcattt ggcggcggcg
    12061 ctattaatga tacattgatg cacctagcga atcctaaatt accatttggt ggtgttggcg
    12121 cctcaggtat gggacgctat catggtaaat attcattcga cacttttaca catgaaaaaa
    12181 gctacatttt caaatctaca cgattagaat caggtgtcca tttaccacca tataaaggta
    12241 aatttaaata catcaaagct ttctttaaaa attaattttt gagccaaaaa taaaccatgc
    12301 ttccataatt tgatgttctt gaaaacaata aatttcaagg tatacataaa ttttttggaa
    12361 acatggtttt ctttttatca tgattaatta aagattaaaa tatgacacat acaccttcag
    12421 atgctacacg agtgttatca catagcgtaa ttttacctgt ttctttattt ctttcgaaaa
    12481 cagttacaac tgaatctcct tgctcatgag cacaaactaa aaggtcatct gatgaggcaa
    12541 tattaaaatc tcttgggaat tgaccaccac tttcagtaat tgttactagt tctaagtgtt
    12601 gaccattatc aagaacttta aaaattgcaa tgctatcatg ccctctatta gatacatata
    12661 agaattgttg atcatgagat aaacgcactg ctgcaagttt agtatctcca tcaaagtttt
    12721 caggaattgt taaatgacgc tcgagctctt caaatttacc gtcattatat tctgcaacac
    12781 tcacagtatt tgataattcg tgtacgacat aagcaaattt tccattatca tgaaattcaa
    12841 tatgtcttgt cccatcacta tctttaaata aagattcttt ataaaattca aacccgttgt
    12901 catcaaattt ataagtaacg atacgatcag cacctaaatc tgttactgca acgtacttac
    12961 catctggagt ttgattaata taatgtgcgt gtggatgatc ttgtctttca tgtgtacctg
    13021 ttggaaaatc atgtgcaagt tcttgaatta gacgtataat ttcacctgtg tgcgtattta
    13081 attcatacat acgtatgatg ccagcaccat ataccgcttc aaataaatat cgtttatctt
    13141 ctgaaatcga tacataacaa cctgtaccag cttttgaaga caaacattta tttaataaat
    13201 gcaattcacc attgtcatca attttcaaac tcgcaacacc gcattgttct ccttctttgt
    13261 tgattccata taaaacttcg ttattacgca ccaaatatgt agacgcttct aattcaaatc
    13321 ctgtttctaa taaatcaata cgtgactgat tttcgtttaa ttcaaaacga taaatccctt
    13381 taccattctt tttagtgtaa gaaccaatat atccatttgt cattactttt acccctccat
    13441 gtcagtctta ttttcatttt attaaatttt agctagaatt aacaatcatt tatcatttcc
    13501 ttaacgctaa aactgtttaa ctactttaaa agaactattt gctgtgatat gtcattattc
    13561 gattttaatt aatccctctt ttttacttac acgagcatta attattaacg cgtttatgct
    13621 tccacaaaat ttttgttgca aaatagaaca catgttcgta taatgtaatt atcagattgg
    13681 ggtgaattaa ggatgacaag ttatgaagaa gaaactgatt atcgtaacgt tccaagagaa
    13741 ttactagata acaatatacc tatgggaaga ggcatgatta aatgggctcc gtttattata
    13801 tgtactaatc tcataaaaaa tctaatttaa tagggttcaa acataattta tatatgtatt
    13861 atgtttgaac cctatatcta tttatttttt actaatatca tttaaaatgt ctcgcaattc
    13921 ttcaatttct ttgttattta attcttcatt tttcgcaaaa ttaagcacta aacttttcat
    13981 atcccctcca tacaatttat taagaaaggt tttagcagtt ttcattttaa tatcatcttc
    14041 tttaat
//
LOCUS       DAHHOJ010000073        13636 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.073, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000073 DAHHOJ010000000
VERSION     DAHHOJ010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13636
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.073"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            484..3438
                     /gene="fdhF"
                     /locus_tag="NBH32_001797"
     CDS             484..3438
                     /gene="fdhF"
                     /locus_tag="NBH32_001797"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCD1515390.1"
                     /translation="MQEHLVVTLDGKDYLVEPGTNLLEFIKSQDTFVPSICYNESMGP
                     IQTCDTCTVEIDGKIERSCSTVIDRPMTVNTVNNDVKDAQKEALDRILEKHMLYCTVC
                     DYNNGDCEIHNTMDAWGLQHQTYEYKEKPYEKDYGPFYRYDPNQCILCGRCVEACQDI
                     EVNETIRIDWDREHPRVIWDNDVPINESSCVSCGQCATVCPCNAMMEVNMEGNAGYMT
                     DTEPGSLAAMIDLTKKAEPGYGPLFAISDSEAEMRKERIKKTKTVCTYCGVGCSFEVW
                     TKDREILKVQPSHDSPANKIATCVKGKFSWGHINSDQRLTKPLVRKNGEFHEVEWDEA
                     LNVIADNFTSIKEKYGPDALSFISSSKATNEESYLMQKLARQVIGTNNVDNCSRYCQA
                     PATKGLFRTVGHGGDSGSIEDLEKAAMSVLIGTNTAEAHPVIASRMKRAQKLFGQKIH
                     VFDIRKHEMAERADRFYQPKPGTDLAWLSAVTKYIIDHDLHDKAFIEEWVEDFDEYYK
                     SLETFTMAFAEEATGIPEAELIKFAEECAKAESVVICWAMGITQQDIGSDSSTAISNL
                     LLVTGNYRRPGTGAYPLRGHNNVQGCSDMGSMPDKITGYQSIEADDIRAKFEKEYGVK
                     LNPKVGKDNHEMVEGVHDGDIHSLYLYGEDTGIVDSNINFVQAAFEKLDFMVVQDEFL
                     TFTATFADVVLPASPSLEKDGTFTNTERRIQRLYQALKPLGDSKPDWKIFQAIANKLG
                     FDWNYKHPSEIMDEIARLTPLYAGVSYDRLEGFNSLQWPVHPDGTDEPILYLEGFNFD
                     NGKAKLFPLSFDNYFKQDEVYDIHVNNGRLLEHFHEGNMTYQTPMLKYKVPRAFVEIS
                     PELAEDRGIHEGAEVKLISETGEAVLQVHVTDRVKGKEIYIPLNNDAMENGDLGAINL
                     LTNSDVDQYTDTPSYKRTSCRLEVITKRGKSPLNPNNFRVNKKRHPQYSVQVQKKWER
                     PDYVFPGNQVDK"
     gene            3438..3911
                     /locus_tag="NBH32_001798"
     CDS             3438..3911
                     /locus_tag="NBH32_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1641 domain-containing protein"
                     /protein_id="HCD1515391.1"
                     /translation="MAERISSKIRRLEKSEEQIKLESLNEVTEAIAANKDSILKAIKL
                     IKVLDDAKILDALNGAVRGRQVIINKFAVELNKDIYTGLLTNMASMVFLLGELNVSDL
                     SDFLNKVNKGLHVANQASPNAKTSVRSLLGVLKDDDMNRSLTYMLNMLKGMSRED"
     gene            4138..4371
                     /locus_tag="NBH32_001799"
     CDS             4138..4371
                     /locus_tag="NBH32_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515392.1"
                     /translation="MLKTSAVISNTLLVIGLVCLFMMKLVLAITFFAVSLSISLVVFN
                     MMFRDRTTMKVIVNASFLIVILAIVVAYFLLSK"
     gene            4393..5169
                     /locus_tag="NBH32_001800"
     CDS             4393..5169
                     /locus_tag="NBH32_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000739218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD1515393.1"
                     /translation="MKKNFKLRISTLLLIVILVVFAVLLIVNETKLFKNDVNYSFDEA
                     VSMQQGKGIVQTKEEDGKFVEANNNEIAKAMTISHKDNDMKYMDITEKVPMSESEVNQ
                     LLKGKGILENRGKVFLEAQEKYEVNVIYLVSHALVETGNGKSELAKGIKDGKKRYYNF
                     FGIGAFDSSAVRSGKSYAEKEQWTSPDKAIIGGAKFIRNEYFENNQLNLYQMRWNPEN
                     PAQHQYASDIRWADKIAQLMDKCYKQFGIKKDDIRQIYYK"
     gene            5480..6604
                     /locus_tag="NBH32_001801"
     CDS             5480..6604
                     /locus_tag="NBH32_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HCD1515394.1"
                     /translation="MKIAIIGAGIGGLTAAALLQEQGHTIKVFEKNESVKEIGAGIGI
                     GDNVLKKLGNHDLAKGIKNAGQILSTMTVLDDKDRLLTTVKLKSNTLNVTLPRQTLID
                     IIKSYVKDDTIFTNHEVTHIDNETDKVTIHFAEQESEAFDLCIGADGIHSKVRQSVNA
                     DSKVLYQGYTCFRGLIDDIDLKHPDCAKEYWGRKGRVGIVPLLNNQAYWFITINSKEN
                     NHKYSSFGKPHLQAYFNHYPNEVREILDKQSETGILLHNIYDLKPLKSFVYGRTILLG
                     DAAHATTPNMGQGAGQAMEDAIVLVNCFNAYDFEKALQRYDKIRVKHTAKVIKRSRKI
                     GKIAQYRSRLVVAVRNRIMKMMPNALAAGQTKFLYKSKEK"
     gene            complement(6696..7649)
                     /locus_tag="NBH32_001802"
     CDS             complement(6696..7649)
                     /locus_tag="NBH32_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase family protein"
                     /protein_id="HCD1515395.1"
                     /translation="MEKVYVAGAIPEVGLKLLQEHFEVEMYEGKGLVDKDTLIKGVKD
                     ATALISLLSTNVDKDVIDAGKDLKIIANYGAGFNNIDIEYAREKSIDVTNTPKASTNA
                     TADLTIGLVLAVARRIVEGDQLSRITGFDGWAPLFFRGREVSGKTIGIIGLGEIGSAV
                     ARRARAFDMDVLYTGPNRKEEKEREIGAKYVDLDTLLKNADFITINAAYNPKMHHLID
                     TEQFKMMKSTAYLINASRGPIVHEQALVQALKDNEIEGAALDVYEFEPDITDDLKSLN
                     NVVLTPHIGNATFEARDMMSKIVANAAISAVQGEKPQFVVN"
     gene            complement(8012..8512)
                     /locus_tag="NBH32_001803"
     CDS             complement(8012..8512)
                     /locus_tag="NBH32_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1515396.1"
                     /translation="MKKLVTATTLTAGIGTALVGHAQHADAAENYTNYNNYNYNTTQT
                     TTTTTTTTTTSSISHSGNLYTAGQCTWYVYDKVGGEIGSTWGNANNWAAAAQGAGFTV
                     NHTPSKGAILQSSEGPFGHVAYVESVNSDGSVTISEMNYSGGPFSVSSRTISASEAGN
                     YNYIHI"
     gene            complement(8749..9171)
                     /locus_tag="NBH32_001804"
     CDS             complement(8749..9171)
                     /locus_tag="NBH32_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4870 domain-containing protein"
                     /protein_id="HCD1515397.1"
                     /translation="MTTEMNDVTNHQPNRKSNDENIMAMLIYLLSLFTSIIGPLIIWL
                     LKKDESKLVDRAGKNYLNYTISYIIWSIILVVITFIGVFLIATDIDFIIIIGFIITFI
                     GILSIFAFSILSFVFTIIACVKYYNGQEYVIPLTIRFI"
     gene            9419..10501
                     /locus_tag="NBH32_001805"
     CDS             9419..10501
                     /locus_tag="NBH32_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HCD1515398.1"
                     /translation="MKIAIVGSGNGAVTAAVDMVSKGHDVKLYCRNQSISKFQNAIEK
                     GGFDFNNEGDERFVKFTDISDDMEYVLKDAEIVQVIIPSSYIEYYADVMAEHVTDNQL
                     IFFNMAAAMGSIRFMNVLEDRHIETKPQLAEANTLTYGTRVDFENAAVDLSLNVRRIF
                     FSTYDRSCLNDCYDKVSSIYDHLVKEESLIKTNLENGNPEVHPGPTLLNVGRIDYAGE
                     FALYKEGITKHTVRLLHAIELERLNLGRRLGFELSTAKESRIERGYLERDKEDEPLNR
                     LFNTSPVFSQIPGPNHVESRYLTEDIAYGLVLWSSLGRVIDVPTPNIDAVIVIASTIL
                     ERDFFEEGLTVESIGLDKLDLEKYLK"
     gene            10596..11996
                     /gene="nhaC"
                     /locus_tag="NBH32_001806"
     CDS             10596..11996
                     /gene="nhaC"
                     /locus_tag="NBH32_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HCD1515399.1"
                     /translation="MMTRKPTFLESISTMIVMVIVVVTGFVFFDIPIQVLLIIASAYA
                     TWIAKRVGLTWQDLEKGIAERLNTAMPAILIILAVGIIVGSWMFSGTVPALIYYGLDL
                     LNPSYFLISAFFISAVTSVATGTAWGSASTAGIALISVGNQLGIPPGMAAGAIIAGAV
                     FGDKMSPLSDTTNLAALVTKVNIFKHIHSMMWTTIPASIIGLLVWFIAGFQFKGHSND
                     KQIQTLLSELAQIYQINIWVWVPLIVIIVCLLFKMATVPAMLISSFSAIIVGTFNHHF
                     KMTDGFKATFSGFNDSMIHQSHISSSVKSLLEQGGMMSMTQILVTIFCGYAFAGIVEK
                     AGCLEVLLTTISKGIHSVGSLICVTVICCIALVFAAGVASIVIIMVGVLMKDLFEKYQ
                     VSRSVLSRTLEDSSTMVLPLIPWGTSGIYYTNQLHVSVGEFFIWTVPCYLCAIIAIIY
                     GFTGIGIKKSSNSRLT"
     gene            12170..12412
                     /locus_tag="NBH32_001807"
     CDS             12170..12412
                     /locus_tag="NBH32_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515400.1"
                     /translation="MTKLIENLKASIFVSTLTKNLSMYSRFDVPKFGCVNGCMLFILY
                     YRVNRIGENRNFEIFTNSIFFIEIKKANKALETLLA"
     gene            complement(12430..13239)
                     /locus_tag="NBH32_001808"
     CDS             complement(12430..13239)
                     /locus_tag="NBH32_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1515401.1"
                     /translation="MKKIATATIATAGFATIAIASGNQAHASEQDNYGYNPNDPTSYS
                     YTYTIDAQGNYHYTWKGNWHPSQLNQDNGYYSYYYYNGYNNYNYNNYNNGYSYNNYSR
                     YNNYSNNNQSYNYNNYNSYNTNSYRTGGLGASYSTSSNNVQVTTTMAPSSNGRSISSG
                     YTSGRNLYTSGQCTYYVFDRVGGKIGSTWGNASNWANAAARAGYTVNNTPKAGAIMQT
                     TQGAYGHVAYVESVNSNGSVRVSEMNYGYGPGVVTSRTISASQAAGYNFIH"
BASE COUNT         4685 a         1914 c         2580 g         4457 t
ORIGIN      
        1 ttatgggtta ttttatttaa tgcctgatat gcggttttgt aaattaaccc attacaaatt
       61 caatttaaaa ataaatcata ccttctaaat gacagtagat atttatcaga cttgggttat
      121 taattttcag aaaaagggta tttataaata gtataacgat ttttaggtga gctacttatt
      181 aagtattgtc attaataagg ttgtgaaaga tatggcaaaa aagcgtgaaa cagataattg
      241 gaaagctaat aatgataatt attttcaatt aaacaaaaag atgatttaat ttcgcgcaaa
      301 atatttcaaa taaaaatgat tgcaaaaata aaaaatacaa aaatacgctt aacattatac
      361 acttaaagta atacaattat attgattcta tctgaaagat gtgtggggca tcgttatttt
      421 aggtggatat gagcaattta ttaaaagtca tttacggaaa aatatataga tggggtgaat
      481 agtatgcaag aacatttggt ggtcacgctt gatggtaaag attatcttgt agaaccaggt
      541 acaaatttac ttgaattcat taaatcgcaa gatacttttg tgccttcaat ttgttataac
      601 gaatcaatgg gaccaattca aacgtgtgat acatgtactg ttgagattga tggtaaaatt
      661 gaacgttcat gtagtacagt gattgatcgt ccaatgacag taaatacagt gaataatgat
      721 gtgaaagatg ctcaaaaaga agcgcttgat cgaattttag aaaagcatat gctgtattgt
      781 acagtatgtg attataataa tggtgattgt gaaattcata atacgatgga tgcatggggg
      841 cttcagcatc aaacgtatga atataaagag aagccatacg aaaaagatta cggcccattt
      901 tatcgttatg atccaaatca atgtatctta tgtgggcgtt gtgtagaagc atgtcaagat
      961 attgaagtaa atgaaactat tagaattgat tgggatcgtg aacatccacg tgttatttgg
     1021 gataatgatg taccgattaa tgaatcttca tgtgtatctt gtggtcaatg cgcaactgta
     1081 tgtccatgta atgccatgat ggaagtgaat atggaaggta atgcgggtta tatgactgat
     1141 acggaacctg gttcattagc ggcaatgatt gatttaacta aaaaagcaga acctggttat
     1201 ggtccactat ttgcaatctc agattcagaa gcagaaatgc gtaaagagcg tattaagaaa
     1261 actaaaacag tgtgtacata ttgtggtgtg ggctgttcat ttgaagtttg gactaaagat
     1321 agagaaattt taaaagtaca accatcacat gattctccag caaataaaat tgcgacttgt
     1381 gttaaaggta agttttcatg gggacatatt aattcagatc aacgcttaac gaaaccatta
     1441 gtaaggaaaa atggtgagtt ccatgaagta gaatgggatg aagcattaaa tgtcattgca
     1501 gataatttta catctattaa agaaaagtat ggcccagacg cactatcatt catttcttct
     1561 tctaaagcga cgaatgaaga atcgtattta atgcaaaaat tagcaagaca agttattggc
     1621 actaataacg ttgataactg ttcaagatat tgccaagcac ctgcaacaaa aggcttattt
     1681 agaacggttg gacacggcgg tgactcaggt agtattgaag atttagaaaa agcagcaatg
     1741 tctgtattga ttggtacgaa tacagccgaa gcacacccgg ttattgcatc acgcatgaaa
     1801 cgtgctcaaa aattatttgg acaaaaaata catgtgtttg atattagaaa acatgaaatg
     1861 gctgaacgtg cggatcgttt ctatcaaccg aaaccaggta cggatttagc gtggttaagt
     1921 gcagtgacga aatatatcat tgatcatgac ttacacgata aagcctttat cgaagagtgg
     1981 gttgaagatt ttgatgaata ttacaagtca ttagaaacat ttacaatggc attcgctgaa
     2041 gaggctactg gtattccaga agcagagttg attaagttcg ctgaagaatg tgccaaagct
     2101 gagtccgttg tcatttgttg ggcaatgggt atcacacaac aagacattgg aagtgattca
     2161 agtacagcaa tttcaaactt attattagtt actggaaatt atcgtcgacc tggtacaggc
     2221 gcgtatccat tacgtggaca taataatgtt caaggttgta gtgatatggg tagtatgcct
     2281 gataaaatta ctggctatca aagtattgaa gcggacgata ttcgcgctaa attcgaaaaa
     2341 gaatatggcg ttaaattgaa tcctaaagtt ggtaaagata atcatgaaat ggttgaaggt
     2401 gtccatgatg gggacattca ttcattatat ctatatggag aagatactgg tattgtagat
     2461 tcaaatatta attttgtaca agctgctttt gaaaaattag atttcatggt agtccaagat
     2521 gaatttttaa catttacagc tacatttgca gatgttgtct taccagcaag tccttcatta
     2581 gaaaaagatg gtacttttac gaatactgag cgacgtattc aacgtttata ccaagcattg
     2641 aaaccattag gtgattctaa acctgactgg aaaatattcc aagcaattgc taataaatta
     2701 ggtttcgatt ggaattacaa acatccaagc gaaattatgg atgaaattgc tagactaaca
     2761 cctctatatg ctggggtaag ttatgatcgt ttagaaggat tcaatagttt acaatggcct
     2821 gtgcatccag atggtacaga tgaaccaata ctttatttag aaggatttaa tttcgataat
     2881 ggtaaagcaa aattattccc attatcattt gataattact ttaagcaaga tgaagtttat
     2941 gatattcatg taaacaatgg tcgattgtta gagcacttcc acgaaggtaa catgacatat
     3001 caaacaccaa tgcttaaata caaagtgcca cgtgcatttg ttgaaatttc tccagagctt
     3061 gctgaagata gaggcattca tgaaggtgca gaagttaagt tgatttctga aacaggagaa
     3121 gcggtattac aagttcacgt gacagatcgt gttaaaggta aagaaatcta tattccatta
     3181 aataatgatg cgatggaaaa tggagaccta ggtgcaatta acttattaac taatagtgat
     3241 gttgatcaat acacggatac accatcttat aaacgtacaa gctgtcgttt ggaggtaatt
     3301 acgaaacgtg gtaagtcacc gttgaaccca aataatttcc gtgtcaataa aaaacgtcat
     3361 ccgcagtaca gtgttcaagt acagaaaaaa tgggaacgtc cagattatgt tttcccagga
     3421 aatcaggtgg ataaataatg gctgagagaa tatcttctaa aattagacgc ttagaaaaat
     3481 cagaagaaca aattaagtta gaaagtttaa atgaagttac agaggcaatt gcagcgaata
     3541 aagatagtat attaaaagca atcaaactta ttaaagtgtt agatgatgct aaaatattag
     3601 atgctttaaa tggcgcagtt agaggacgtc aagtgattat taataaattt gctgttgagc
     3661 tcaacaaaga tatctatact ggtctattaa caaatatggc atcaatggta tttttattag
     3721 gtgaattaaa tgtatcagat ttaagtgact tccttaataa agttaataaa ggattacatg
     3781 ttgcaaatca agcgagtcct aatgcgaaga catctgtaag aagtctgtta ggcgttttaa
     3841 aagatgacga catgaaccgt agtttaacat atatgcttaa tatgctaaaa ggtatgtctc
     3901 gagaagatta acattacaat gaaaaagtgt ttctacatgc aagcagtgta ggagcacttt
     3961 tttaattttt ttaaaaatta aaaagggtgt ttttgatgga ataaatgaat aacattgaaa
     4021 aacaaaaaaa tcacgtgcta ttatgaatca atcatcgaca ggcctatgaa acatcatgta
     4081 ttattatgat tatgtaagtt atttaaataa aaagggtgag tgcatattaa ttcgagaatg
     4141 ttaaaaacgt cagctgtaat ttctaataca ttattagtca taggtttagt ctgtctattt
     4201 atgatgaaat tagtgttagc tattactttt tttgctgttt cattatcaat tagtctagtt
     4261 gtctttaata tgatgttcag agatagaaca acgatgaaag taatcgttaa cgcttctttt
     4321 ttaatcgtaa tattagcgat tgtagtggca tattttttat tgtctaagta agaaaaatga
     4381 ggttttgtct atatgaagaa gaatttcaag ttacgcattt caacgctact attgatagtt
     4441 attttagttg tttttgctgt attactcatc gtgaatgaaa ctaaattgtt taaaaatgat
     4501 gtgaattact cttttgatga ggctgtttca atgcaacaag ggaaaggtat tgtacagaca
     4561 aaagaagagg atggtaaatt tgttgaagca aataataatg aaattgctaa agcaatgact
     4621 atttcacata aagacaatga tatgaagtat atggatatca cagaaaaagt gccaatgtcg
     4681 gaatctgaag ttaaccaatt gctaaaaggt aaggggattt tagaaaatcg agggaaagtt
     4741 tttctagaag ctcaagaaaa atatgaggtt aatgtcattt atcttgttag ccatgcatta
     4801 gtagaaacag gtaacggcaa atcagaatta gcaaaaggca ttaaagatgg aaaaaaacgc
     4861 tattacaact tttttggtat aggagcattc gatagtagtg ctgttcgtag tggaaaaagt
     4921 tatgctgaaa aggaacaatg gacatcacca gataaggcga ttattggtgg tgcaaagttc
     4981 attcgtaatg aatattttga aaacaatcaa ctgaatttat atcaaatgcg atggaatcca
     5041 gaaaatcctg cgcaacatca atatgcgagt gacattcgct gggcagataa aattgcccaa
     5101 ttaatggata aatgctataa gcagtttggt ataaagaaag atgatattag acaaatatat
     5161 tataaataag acatcagtgc ttaaaggagc tggaacaatt tattgtttcg agctccttta
     5221 gcgcattctg agtgtgttag ttaaatggat tttaacctaa caaaaaacgc tatatagcat
     5281 caaatatgct atatcccaca tcattgttac aaatgtacat tatgtaaatg aatattgctg
     5341 tctaaatgtg catgtaatat acaatggtgc agataataca cttaagtctt taaaaatgaa
     5401 acgttagttc caagagtcat ttttaaacaa tagtgcatgt gataaaatag aaaagaatga
     5461 aaaatataga ggtgacaata tgaagatagc aattataggt gcaggcatcg gtggattaac
     5521 agctgctgca ttattacaag aacaaggtca tactattaaa gtctttgaaa aaaatgagtc
     5581 agttaaagaa attggcgctg ggattggtat cggagataat gtgcttaaaa aactaggtaa
     5641 tcatgactta gctaaaggta ttaaaaatgc tgggcaaatc ttatctacaa tgacagtgtt
     5701 agatgacaaa gatcgcctgt taactactgt taaattaaaa agtaatacat tgaatgtgac
     5761 gttaccacgc caaacattaa ttgacattat taaatcttat gtaaaagatg atacaatatt
     5821 tacaaatcat gaagtcacgc atatagataa tgagacagat aaagttacca tacatttcgc
     5881 ggaacaagag agtgaagcat ttgatttatg tattggtgct gatggaattc attctaaagt
     5941 gagacaatct gtaaatgctg acagtaaagt attatatcaa gggtatacat gctttagagg
     6001 tttaattgat gatattgatt taaagcatcc ggattgtgca aaagaatact ggggaagaaa
     6061 aggaagagta ggtattgttc cgttattaaa taatcaagca tattggttca ttacaatcaa
     6121 ctcgaaggaa aacaatcata aatatagttc gtttggtaaa cctcatttgc aagcatactt
     6181 taatcactat ccaaatgaag ttagagagat cttagacaaa caaagtgaaa caggtatctt
     6241 attgcataat atttatgatt tgaaaccact caaatctttt gtttatggtc gtactatttt
     6301 actaggagat gcagcacatg cgacaacgcc taatatgggg caaggtgctg gacaagcaat
     6361 ggaagatgct atcgtattag taaattgttt taacgcatac gactttgaaa aagcattaca
     6421 gcgttatgat aaaatacgtg tcaaacatac tgcaaaagta attaagcgtt ctagaaaaat
     6481 cggtaaaatt gcccaatatc gtagtcgttt agttgttgca gttagaaatc gtattatgaa
     6541 aatgatgcca aatgcattag cagctggaca aactaaattc ttatataaat cgaaagagaa
     6601 ataatacaac aatatgaaaa cccccgtatg ttgaaatgag agctcaacat atgggggttc
     6661 ttgtttttat aatgtcatta taataaattc aattattagt taacgacaaa ttgtggtttc
     6721 tcaccttgaa cggcactaat tgcagcatta gcaacaattt tagacatcat gtcacgtgct
     6781 tcaaatgtag cattaccaat atgcggtgtt aatactacat tattaagtga ttttaagtca
     6841 tcggtaatat ctggttcaaa ttcatataca tcaagtgcag caccttcaat ttcattatct
     6901 ttcaatgctt gcactagtgc ttgttcgtgc acgattggac cacgagaggc attgattaaa
     6961 tacgccgtag atttcatcat tttaaattgt tctgtatcaa ttaaatgatg cattttagga
     7021 ttataagcag cgttgatagt gataaaatct gcattcttta atagtgtatc taaatctaca
     7081 tattttgcac cgatttctcg ttctttttct tctttgcgat taggtcctgt gtatagtaca
     7141 tccatgtcaa atgctcttgc acgacgcgct actgcactac caatttcacc taaaccgata
     7201 atgccgattg ttttcccaga tacttctcta cctctgaaaa ataaaggtgc ccatccgtca
     7261 aatccagtta tacgtgataa ttggtcgcct tcaacaatac gacgcgctac tgcaagtact
     7321 aatccaattg ttaaatcagc agtcgcgttt gttgatgctt taggtgtgtt tgtaacatct
     7381 atactttttt ctcgagcata ctcgatatca atattattaa aaccagcacc atagttggca
     7441 atgattttta agtctttacc agcatcgata acatctttat caacgtttgt agataataaa
     7501 ctaattaagg cagtcgcatc tttaacacct ttaattaaag tgtctttatc gactaatcct
     7561 ttaccttcat acatttcaac ttcaaaatgt tcttgtaaaa gttttaaacc tacttctggt
     7621 attgcaccag caacataaac tttttccata aaagatcact ccttttatct tagtatagta
     7681 gaagattaga cagtatacaa ctatgtcatg atgtcttgtg tatcaatgat gtaagcgtgt
     7741 acttttgatg gaggtgatat aacttaggca ctgtagaact atgaatattg taatgtggaa
     7801 aaactggatc aattaaatta gataacgtag ttttaaagtt aaatagtatt gaaaaaatta
     7861 atattatgaa tatggggggg aaatataaat aagtaggtgg caacgaaaaa tagcaaaaaa
     7921 agagcttctc ctataaagga aagctcaaag ttttttgatg acatatgtac tagaattaag
     7981 tttcaagaca atatgtatca tcgtgtttat attaaatatg gatgtagttg tagttacctg
     8041 cttcgcttgc agaaatagtt ctagaactta ctgagaaagg tccgccacta taattcattt
     8101 ctgaaattgt aactgaacca tcactgttta cactttctac atatgcaacg tgaccaaatg
     8161 gtccttcaga agattgtagg atagcgcctt tagaaggtgt atggtttact gtgaatccag
     8221 caccttgtgc agcagcagcc caattattag catttcccca agtagaaccg atttctccgc
     8281 ctactttatc atatacatac caagtacatt gtcctgcagt gtataagtta ccagaatgtg
     8341 aaattgatga tgtagttgtc gtagttgttg tagtcgttgt agtttgagtc gtattatagt
     8401 tatagttgtt ataatttgta taattttcag cagcgtctgc atgttgtgca tgacctacta
     8461 atgctgtacc aattcctgct gttaacgtag ttgctgttac taattttttc atgaataaag
     8521 tcctccaaag ttctatatct ttttttataa ataaaacgta gcgactgttt tattctcaca
     8581 tctcgaattg atgacaatag ttactttaac aaaattaatg cttcttgtgg ggaatgttat
     8641 tgatttgtaa aagaataaaa aaactttgac taattttgta ataaaaatta gtcaaagtta
     8701 caatgagatt aacagataat taataggaaa tatttatttg taatatgttt aaataaatcg
     8761 aattgttaaa ggtattacat attcttggcc attataatat ttgacacacg caataattgt
     8821 gaatacaaaa gataatattg agaaagcgaa tatggataaa ataccgataa acgtaatgat
     8881 gaaacctata atgataatga aatcaatatc ggtagcaatt aggaaaacgc caataaaagt
     8941 gataacgact aaaatgatag accaaataat ataagaaatc gtatagttaa gataattttt
     9001 tccagcacga tcaactagtt ttgattcatc ttttttcaat aaccatataa tcagtggacc
     9061 aataatagat gtgaataaac ttaataaata gataagcatc gccataatgt tctcatcatt
     9121 ggatttgcga ttcggttgat gatttgttac gtcgttcatt tcagttgtca tattagacac
     9181 tcctttgaaa attgtaatat tatctttaac tataacaaaa tataatcaaa aataaacatg
     9241 tttattaaac aattattaaa aataaaaata attggtggac gtcggcgttt aaataggtta
     9301 atttaaggtt atatatactt aacatttata atgatgcgta atgaattcgc atcattttta
     9361 tattgtctta cgtataattt gtttttaatt ttaaccaaag atagaaagag ggttgtttat
     9421 gaaaatagca attgtaggat caggaaatgg cgcagttacg gcagcagtag atatggtgag
     9481 caaaggccac gatgttaaat tatattgtcg taatcaatct ataagtaagt ttcaaaacgc
     9541 aatcgaaaag ggcggatttg attttaataa tgaaggtgat gaacgtttcg taaaattcac
     9601 tgatattagt gatgatatgg aatatgtttt aaaagatgct gaaattgttc aagtgattat
     9661 tccatcttca tacatagagt attatgctga tgtaatggca gagcatgtaa ctgataatca
     9721 gttgatattc ttcaacatgg ctgcagcaat ggggtcaatt cgttttatga atgttttaga
     9781 agatagacat attgaaacaa aaccacaatt agcggaagct aatacgttga cgtatggtac
     9841 gcgtgtcgat tttgaaaatg cagcagttga tttatctcta aatgtacgtc gtatcttctt
     9901 ttcaacatat gatagaagct gtctaaatga ttgttatgac aaagtttcaa gtatttatga
     9961 tcatttagta aaagaggaaa gcttaattaa aacaaatctt gaaaatggta atcctgaagt
    10021 gcatccaggg ccaacattat tgaatgtcgg tcgcattgat tatgctggtg agttcgcttt
    10081 atataaagaa ggaattacta aacatacagt tagattactt catgcaatcg aattagaacg
    10141 tttaaattta ggccgtagat taggttttga attatcaaca gctaaagaat cacgtattga
    10201 acgtggttat ttagaacgtg ataaagaaga tgaaccatta aatcgtttgt ttaatacaag
    10261 cccagtattt tcacaaattc caggaccaaa tcatgtagaa agcagatatt taactgaaga
    10321 tattgcatat ggtttagtat tatggtcaag cttaggtcgt gttattgatg taccgacacc
    10381 aaatatagat gcagtaattg tcattgcatc gacaatctta gaaagagact tctttgagga
    10441 aggcttaaca gttgaatcca ttggcttaga taagcttgat ttagaaaaat atttaaaata
    10501 aatgatggct tgaagataga aaaggatata gcattatgta aaagcaataa attgaagaaa
    10561 agaggtttct catcaataag cgaaggggac gatagatgat gacaagaaaa ccaacctttt
    10621 tagaatcaat ttcgacaatg attgtaatgg ttattgttgt tgtaacaggc tttgtgtttt
    10681 ttgatattcc aattcaagta ttattaatta ttgcctcagc atatgccaca tggattgcaa
    10741 aacgtgtcgg cttaacatgg caagatttag aaaaaggcat tgcagaacgt ttaaatactg
    10801 caatgcctgc aattttaatt atactagcgg taggaattat agtaggcagt tggatgtttt
    10861 ctggcacagt gccagcgttg atttattatg gcttagattt attaaatcca agctattttt
    10921 taatatcagc cttttttata agtgctgtta catctgtagc aactggtaca gcatggggct
    10981 ctgcatcaac tgcagggatt gcacttattt ctgttggtaa tcaattgggg attcctccag
    11041 gaatggctgc gggtgctatt atagcaggcg ctgtatttgg agataaaatg tcaccattat
    11101 cagatacaac taatttagcg gcacttgtta ctaaagttaa tatatttaaa catatacatt
    11161 cgatgatgtg gacgacgata cctgcgtcaa tcataggttt attagtatgg tttattgctg
    11221 gatttcaatt taaagggcat tcaaatgata aacagattca aactttgtta tcagagcttg
    11281 cacagattta tcaaattaac atatgggtct gggttccctt aattgtgatc attgtttgtt
    11341 tgctatttaa aatggctaca gtgccagcta tgctaatatc aagcttttct gccattatag
    11401 tgggtacttt taatcatcat ttcaaaatga cagatggttt caaagcaaca tttagtggct
    11461 ttaacgactc aatgatacat cagtctcata tttcatccag tgtgaaaagc ttgttagagc
    11521 agggtggtat gatgagtatg actcaaatat tagtaacgat attttgcgga tatgcatttg
    11581 caggtattgt agaaaaagca ggatgtttag aagtcttatt aactactatt tctaaaggca
    11641 tccattctgt aggaagttta atatgtgtta ctgttatttg ttgtattgcg cttgtattcg
    11701 ctgcaggtgt tgcttcgatt gtaattatta tggtcggtgt gttaatgaaa gatttgttcg
    11761 aaaaatacca agtctctcgt tccgttttat caagaacact tgaagactca agtacgatgg
    11821 tcttaccttt aataccatgg ggaacatcag gtatttacta tacgaatcaa cttcatgtct
    11881 ctgttggaga atttttcata tggacagtac catgttattt atgcgcaatt atagcaatta
    11941 tctatggttt tacagggata ggtattaaaa agtcatcgaa ttcacgttta acttaatgtg
    12001 agcgtggaat atatataata tgttgaaaca ctttaatcat ttataattgt agcggttata
    12061 atttgaaaag gttttaactt agaataaata tcctctatac atatactgaa tatgttttgt
    12121 agcggaacat gttgatatat gtaatgtaag ttttatgtca tgatttgtaa tgactaaatt
    12181 aattgagaat ttgaaggcaa gtatatttgt aagtacttta actaaaaatt tatcaatgta
    12241 tagccgattt gacgtgccta aatttgggtg tgtcaatggc tgtatgttgt ttattcttta
    12301 ttacagagtg aatcggattg gtgaaaatcg aaattttgag atttttacca attcgatttt
    12361 tttcatagaa attaaaaaag ccaacaaggc tcttgaaacc ttgttggcgt aaaacttagt
    12421 catcactaat tagtgaatga agttataacc agcagcttgg ctagctgaga ttgtacgtga
    12481 agttacaaca cctgggccat aaccatagtt catttctgaa actcttactg aaccattgct
    12541 gttaacactt tcaacgtatg caacgtgacc gtatgcacct tgagttgttt gcataattgc
    12601 accagctttt ggtgtattgt tcactgtgta accagctctt gcagctgcgt tagcccagtt
    12661 acttgcattg ccccaagttg aaccgatttt accacctaca cgatcaaata cgtagtatgt
    12721 acattgacca gaagtgtata agttacgtcc tgaagtataa ccacttgaga ttgaacggcc
    12781 atttgatgat ggagccatag ttgtagttac ttgaacattg ttgcttgaag tgctgtagct
    12841 tgcacctaaa ccaccagtac ggtagctgtt tgtgttgtaa ctattatagt tattgtagtt
    12901 atatgattga ttattatttg agtagttgtt gtaacggctg tagttattgt agctataacc
    12961 gttgttgtaa ttgttatagt tatagttatt gtaaccattg tagtagtaat agctgtagta
    13021 gccattatct tggtttaatt gacttggatg ccagttacct ttccatgtgt aatggtagtt
    13081 accttgtgca tcaatagtgt aagtatagct atatgatgtt gggtcatttg gattataacc
    13141 gtagttatct tgctcagaag catgagcttg atttcctgat gcaattgcga ttgtagcgaa
    13201 tcctgcagtt gcgatagtag ctgtagcgat tttcttcatt ttaaaaatat cctcctaaaa
    13261 attttaaatc taaaatattt tcgtaatgtc cgtgtgacaa aattaatgtt ataagttatc
    13321 tctcgtaatt aaacgacaag aaagactata acagaaatta gcgtccttgt gtgctttgtt
    13381 aacgttttgt aattttttgc taatatcttg acacaataga attttaaaag tatagaaatt
    13441 tgcattttgc aaaacttata actacggcat tctttgtgaa aactgaatgt ttcgaaaata
    13501 agtctgttac aaatttgtaa tattactgaa aattctaaat gtatattttg tgcataatat
    13561 aggactttta atcagaaaat gcataaaaat tcgacacgaa cgatcaagtt tctttgatta
    13621 tgaaagcttt ggtatt
//
LOCUS       DAHHOJ010000074        13239 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.074, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000074 DAHHOJ010000000
VERSION     DAHHOJ010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 13239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.074"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(182..1048)
                     /locus_tag="NBH32_001809"
     CDS             complement(182..1048)
                     /locus_tag="NBH32_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001005521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCD1515402.1"
                     /translation="MNEQWLEHLPLKDIKEISPVSGGDVNEAYRVETDTDTFFLLVQR
                     GRKESFYAAEIAGLNEFERAGITAPRVIASGEVNGDAYLVMTYLEEGASGSQRQLGQL
                     VAQLHSQQQEEGKFGFSLPYEGGDISFDNHWQDDWCTIFVDKRMDHLKDELLNRGLWD
                     ANDIKVYDKVRRQIVAELEKHQSKPSLLHGDLWGGNYMFLQDGRPALFDPAPLYGDRE
                     FDIGITTVFGGFTSEFYDAYNKHYPLAKGASYRLEFYRLYLLMVHLLKFGEMYRDSVA
                     HSMDKILQDTTG"
     gene            1266..2330
                     /locus_tag="NBH32_001810"
     CDS             1266..2330
                     /locus_tag="NBH32_001810"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCD1515403.1"
                     /translation="MYKLIKPFLFKIEPEKAHGLTIDALKTLQKFPVLFPVVDKLFTY
                     KNPTLSQTIQGNTYDNPIGLAAGFDKSCEVPKALEHLGFGALELGGITPKPQPGNPQP
                     RMFRLLEDDALINRMGFNNIGMNKALSHLRKNAYQVPVGINVGVNKMTPYEARYQDYI
                     KVIDTFKHDVSFFTVNISSPNTENLQNFHDKDEFSMLCQALTAFKKQHDVTVPIYLKL
                     TSDMDFDGLKALLPAITETFDGIILANTTRQRDGLTSANKVEEGGLSGRSLFERNLKL
                     IKYAYQQTNGEFLIIGTGGVFSTEDAIKMMRHGASLIQIYSSLVIEGPGLTKKMNKGI
                     ARYLKDHHFDNVSDIIGLDA"
     gene            2485..2760
                     /locus_tag="NBH32_001811"
     CDS             2485..2760
                     /locus_tag="NBH32_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515404.1"
                     /translation="MTKETDNKKTVATSDNKDTQQQNQPEEDERQGLNGYRKTDLDLE
                     IEQELREMMKTGENETKNDYKKFKVFSLISTLVIVILAIIRFVHKMM"
     gene            complement(2946..3668)
                     /locus_tag="NBH32_001812"
     CDS             complement(2946..3668)
                     /locus_tag="NBH32_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCD1515405.1"
                     /translation="MINAEPIISKMKNQKINYDKVLKKLIGQWEREAIRPKILLHSCC
                     APCSTYTLEFLTQYADIAIYFANSNIHPKNEYLRRAKVQEQFVEDFNRKTGANVKYIE
                     APYEPHKFVKMVKDKELADEKEGGLRCTACFEMRLDIVAKAAVEHGYDYFGSAITLSP
                     KKNAQLINELGMDVQKIYDVNYLPSDFKKSKGYERSIEMCNDYNIFRQCYCGCVFAAM
                     QQGIDFKTVNKEAKAFLEQYPD"
     gene            4022..4471
                     /locus_tag="NBH32_001813"
     CDS             4022..4471
                     /locus_tag="NBH32_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HCD1515406.1"
                     /translation="MTALFPYIAFKNSKEALAYYEEVFGATDVKRLEVGEEQASHFGM
                     TKEEAQEATMHAEFEVLGVKVLCSDSFGRADKINNGISLLIDYDVNNKEDADKVEAFY
                     EQIKDHSSIEIELPLADQFWGGKMGIFTDKYGVRWMLHGQDYTAIQQ"
     gene            complement(4552..5139)
                     /locus_tag="NBH32_001814"
     CDS             complement(4552..5139)
                     /locus_tag="NBH32_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1515407.1"
                     /translation="MTIDRRIRKSQVAMRNVFIELLHQHQLEEITVQQIADLADVNRS
                     TFYTHYYDKYDLLEKLENQQLEEIRDFIHDEKLNGAVKLSTDNIHQIMTVLIEMIEKD
                     IAFYQLMFRMGKDSNIHEKLYDLIMCHLQRYKNENDQINGIPFSYFMSYVSGAGLSLI
                     RHWVEDPNRIDKVDLIRHFNTIVNQGPATIIKNIQ"
     gene            5282..6964
                     /locus_tag="NBH32_001815"
     CDS             5282..6964
                     /locus_tag="NBH32_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HCD1515408.1"
                     /translation="MNQHLLGNPKLTVTHVNEVKAGINHIVVDSVQYGNQEMIMEKDV
                     TVEMRDGEKLYINIFRPNKDGKFPVVMSADTYGKDNKPKITNMGALWPTLGAIPTSSF
                     TPEESPDPGFWVPNDYVVVKVALRGSDKSKGVLSPWSKREAEDYYEVIEWAAKQPWSN
                     GNIGTNGVSYLAVTQWWVASLNPPHLKAMIPWEGLNDMYREVAFHGGIPDTGFYRFWI
                     QGIFARWTDNPNIEDMIQAQQEHPLFDDFWKQRQVPLSQIKTPLLTCASWSTQGLHNR
                     GSFEGFKQAASEEKWLYVHGRKEWESYYARENLERQKSFFDFYLKEENNDWKDTPHVI
                     YEVRDQFYKGEFKSASAFPLPNTEYTPLYLNAENHTLNHAKISSAHVAQYDSEDKRQD
                     VSFKYTFDKDTELVGNMNLKLWVSTKDSDDMDLFAGIKKLDRRGNEVNFPDFNHIENG
                     QVATGWLRVSHRELDQEKSTIAQPWHKHETELKLSQDEIVPVEIELLPSGTLFKQGET
                     LEVVVKGSEIVIGNSTPGMKTRYEHEETVNKGMHMIYTGGKYDSQLIIPIVN"
     gene            complement(7073..7708)
                     /locus_tag="NBH32_001816"
     CDS             complement(7073..7708)
                     /locus_tag="NBH32_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1515409.1"
                     /translation="MKKVISLFVAILSVLIFTDNANAENNKPEGVNENSNLIPVRQPD
                     ANYPGPVSDIARVKKLDNLIDGTKDPDVIKQLKDDNEVPNNAVKAKDSKIVSDCRHLL
                     KHPDNLMDGTKDPNVINQLKNANGTSNNSAVNPSIKANDIHTKSNKQMAKQQSIHLDS
                     KSKSVHKQQLKELPKTGVNNESNTAIMVLFIGLILLVVSLVTRFIKLNSHQ"
     gene            complement(7724..8800)
                     /locus_tag="NBH32_001817"
     CDS             complement(7724..8800)
                     /locus_tag="NBH32_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HCD1515410.1"
                     /translation="MTKIFKISFLILLVVIVSSYYGESFVLADNNPIVNSENGSDNNF
                     ESGTIERNQNELYEVGDYSTPLTERVFGKDQRTVVNNILQRPYKQTVLLNMTFSNNRV
                     YKGTGTMIGKDIVLTAAHNVYSKDDKGWAKKIDVYAGVNGQTYTIGKAFSHKFFVSKS
                     WINNAPTKEDIAIIKLNSNLGNKTGYLTLNTRISKGENIEISGFPGDKSDNRQYKGKG
                     KLESFDENEMYYTVDTFSGQSGSAIRDSKNNIIGVHAYGRYNHNSGVRINDLKLDYIN
                     YWIGKYRSHPYNKKVKVIKSKYIYWKNIEITKKGDNKLIKKDKAYTAKLYYNIPNGYK
                     YYSLYDEKNRWMGYFNSNDIKVVK"
     gene            complement(9143..9526)
                     /locus_tag="NBH32_001818"
     CDS             complement(9143..9526)
                     /locus_tag="NBH32_001818"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015901315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCD1515411.1"
                     /translation="MIRTMMNAKIHRARVTESNLNYVGSITIDSDILEAVDILPNEKV
                     AIVNNNNGARFETYVIAGERGSGKICLNGAASRLVEVGDVVIIMTYAQLNEEEIKNHA
                     PKVAVMNEDNVIIEMIHEKENTIVL"
     gene            complement(9528..10379)
                     /locus_tag="NBH32_001819"
     CDS             complement(9528..10379)
                     /locus_tag="NBH32_001819"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCD1515412.1"
                     /translation="MTKLITTVKEMQHIVKAAKRSGTTIGFIPTMGALHDGHLTMVRE
                     SVSTNDITVVSVFVNPLQFGPNEDFDAYPRQIDKDLELVSEVGADIVFHPAVEDMYPG
                     ELGIDVKVGPLADVLEGAKRPGHFDGVVTVVNKLFNIVMPDYAYFGKKDAQQLAIVEQ
                     MVKDFNHAVEIIGIDIVREADGLAKSSRNVYLTEQERQEAVHLSKSLLLAQALYQDGE
                     RQSKVIIDRVTEYLESHISGRIEEVAVYSYPQLVEQHEITGRIFISLAVKFSKARLID
                     NIIIGAE"
     gene            complement(10372..11190)
                     /gene="panB"
                     /locus_tag="NBH32_001820"
     CDS             complement(10372..11190)
                     /gene="panB"
                     /locus_tag="NBH32_001820"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCD1515413.1"
                     /translation="MKTVSQLIDMKQKQTKISMVTAYDFPSAKQVEAAGIDMILVGDS
                     LGMTVLGYESTVQVTLADMIHHGRAVRRGAPNTFVVVDMPIGAVGISMTQDLNHALKL
                     YQETNANAIKAEGAHITPFIEKATAIGIPVVAHLGLTPQSVGVMGYKLQGATKEAAEQ
                     LILDAKNVEQAGAVALVLEAIPNDLAEEISKHLTIPVIGIGAGKGTDGQVLVYHDMLN
                     YGVEHKAKFVKQFADFSVGVDGLKQYDQEVKSGAFPSEEYTYKKKIMNEVNNND"
     gene            11263..12123
                     /locus_tag="NBH32_001821"
     CDS             11263..12123
                     /locus_tag="NBH32_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCD1515414.1"
                     /translation="MLSVAIIGPGAVGTTIAYELQQSLPHTTLIGRHAKTITYYTVPH
                     APAQDIVVKGYEDVTNTFDVIIIAVKTHQLDAVIPHLTHLAHEDTLIILAQNGYGQLE
                     HIPFKNICQAVVYISGQKKGDVVTHFRDYQLRIKDNALTRQFRDLVQDSQIDIVLEAN
                     IQQAIWYKLLVNLGINSITALGRQTVAIMHNPEIRTLCRQLLLDGCRVAQAEGLNFSE
                     QTVDTIMTIYQGYPDEMGTSMYYDIVHQQPLEVEAIQGFIYRRAREHNLDTPYLDTIY
                     SFLRAYQQNM"
     gene            complement(12209..12913)
                     /gene="budA"
                     /locus_tag="NBH32_001822"
     CDS             complement(12209..12913)
                     /gene="budA"
                     /locus_tag="NBH32_001822"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HCD1515415.1"
                     /translation="MTNVLYQHGTLGTLMAGLLEGTATINELLEHGNLGIATLTGSDG
                     EVIFLDGKAYHANEHKEFIELKGDEKVPYASITNFKASKTFPLQQLSQDDVFAQIKNE
                     MLSENLFSAVKIYGTFKHMHVRMMSAQQPPYTRLIDSARRQPEEKRQDIRGAIVGFFT
                     PELFHGVGSAGFHIHFADDERAYGGHVLDFEVDDVVVEIQNFETFQQHFPVNNETFVK
                     AKIDYKDVAEEIREAE"
BASE COUNT         4243 a         2518 c         1971 g         4507 t
ORIGIN      
        1 aaaagttaga aaatcacttt tattgcaact caatcttgtt ttgcattata gaaattaaca
       61 atagagatta aaattttatt ttccaacaat aaaaaagcac aaatattttt attgaggcag
      121 ctgaaggcct tctcattgta aaaatatctg tgcatattat tatcttaatc taacgtaata
      181 actaacctgt tgtatcttgt aaaatcttat ccatagagtg cgcaacacta tcacggtaca
      241 tctccccaaa tttcaataaa tgcaccatca ataaatataa acgataaaat tcaagtctat
      301 aggatgcacc ttttgcaagt ggataatgtt tattatacgc atcataaaat tcgctcgtaa
      361 aaccaccaaa taccgttgta ataccgatat caaattctct gtcaccatat aatggtgctg
      421 gatcaaataa cgccggacga ccatcttgta agaacatata attgccaccc cataggtcac
      481 catgtaataa agacggttta ctttgatgct tttctaattc cgccacaatt tgacgtcgca
      541 ctttgtcata tactttgata tcgttggcat cccatagccc tctatttagc aactcatctt
      601 tcaaatgatc catgcgcttg tcgacaaaaa tggtacacca atcgtcttgc caatgattat
      661 caaaagaaat atcgccacct tcataaggta atgagaagcc aaatttgcct tcttcttgtt
      721 gctgactgtg taattgagct acgagttgcc ctaattggcg ttgactccct gaagcccctt
      781 cttctaaata cgtcatcact aaatacgcat caccgttaac ctcgccactt gcaattactc
      841 taggtgccgt gatacctgca cgttcaaatt catttaaacc tgcaatttct gcagcataaa
      901 atgattcttt acgtccacgt tggacaagta agaaaaatgt atccgtatcc gtttcgactc
      961 gatatgcttc gtttacatcg ccaccactca ctggtgaaat ctctttaata tcttttaaag
     1021 gtaaatgctc taaccattgt tcattcatac aaacaccttc cattacgctt actttatact
     1081 attatattgc ctaattaaga tgatttaatc tcaattttat ttgtggtact tgcgtcaatt
     1141 ttcaaaactt tttagtttta actaggaaat tatgtaggga aatgctagtc tattaaaaga
     1201 atatatttca catatattaa gttgaggata gtaagcctca aatataatga aataggagca
     1261 tactcatgta caaattaatt aaacctttct tattcaaaat cgaacccgaa aaagcacacg
     1321 gactaactat cgatgcatta aaaacgttac aaaaatttcc ggttttattc ccagtcgtcg
     1381 ataaactatt tacttataag aatccaacgt tatcacaaac gatacaaggt aatacgtacg
     1441 acaatccaat tggcttagca gctggtttcg acaaatcttg cgaagtacca aaagcattgg
     1501 aacaccttgg attcggtgct ttagaattag gtggtatcac acctaaacct caaccgggta
     1561 accctcaacc acgcatgttc agattattag aagatgatgc cttgataaat cgtatgggct
     1621 tcaataatat tggtatgaac aaagcactca gtcatttgcg taaaaatgct tatcaagtac
     1681 ctgttggtat caatgttggt gtgaataaaa tgacacctta tgaagcgcgt tatcaagatt
     1741 atataaaagt cattgatacg tttaaacacg acgtttcatt tttcacagtc aatatcagtt
     1801 ctccaaatac tgaaaatctt caaaacttcc atgataaaga tgaattttca atgttatgcc
     1861 aagctttaac agcatttaaa aaacaacatg atgtaacagt gccaatttat ttaaaactaa
     1921 cgtctgatat ggatttcgat ggcttaaaag cactattacc agcgattact gagacatttg
     1981 acggtatcat cttagcaaac acaacgcgac aacgagatgg tttaacttct gctaataaag
     2041 tcgaagaagg cggtttgagt ggtcgttcat tatttgaacg taatttaaaa ttgattaagt
     2101 atgcttatca gcaaacaaat ggtgaatttt taattatagg tacaggcggc gtattcagta
     2161 ctgaagatgc aatcaaaatg atgcgtcacg gtgcgtcact tattcaaatt tattcatcac
     2221 ttgttattga aggcccaggt ttaactaaga aaatgaacaa aggcatcgca cgttacttaa
     2281 aagatcatca ttttgacaat gtcagtgata ttataggact agatgcctaa tccaacgtag
     2341 tcaaataata ctttaaaact tgctgaatcg tcttattaaa attatgatat aactcaagaa
     2401 gatatattta tcaccatttt tcaaaataat ggcttaaata tcacatatca ttaaatatag
     2461 cgttttataa cggaggcgtt tgttatgact aaagaaacag ataataaaaa aacagtagcg
     2521 acttctgaca acaaagacac acagcaacaa aatcaacctg aagaagatga acgtcaaggt
     2581 ttgaatggtt atcgtaaaac agatcttgat ttagaaattg agcaagagct acgtgaaatg
     2641 atgaaaacag gagaaaatga aacgaaaaat gactacaaaa agtttaaagt gttttcactt
     2701 atttcaacac ttgtcattgt cattttagca attataagat ttgttcataa aatgatgtaa
     2761 ttagagtgag acattgtttt atgtctcagg atccagttat ttattatagc tacaatattt
     2821 acgatgatat aaataacccg agattttagt atgattcatt tcactaaagt ctcgggtttc
     2881 tatttgataa tttttaatag ggcatggctt gtatatgcgc ctgcctttta tctcatttcc
     2941 aatgattaat ctggatattg ttctaaaaat gctttcgctt ctttattaac tgttttaaaa
     3001 tcaatacctt gttgcatcgc tgcaaagaca catccacaat aacactgcct aaagatatta
     3061 tagtcattac acatttctat ggaacgctca taacctttac ttttcttaaa atcacttggc
     3121 aaatagttca catcgtatat tttttggaca tccataccaa gttcattgat taattgtgcg
     3181 ttctttttag gtgataatgt aattgcactg ccaaaataat catagccatg ttctacagct
     3241 gctttcgcta caatatccaa acgcatttca aaacaagcgg tacaacgtaa gccgccttct
     3301 ttttcatcag ctaattcttt atccttcacc attttcacaa acttatgcgg ttcataaggt
     3361 gcttcaatat acttcacatt cgcaccagtc ttgcgattaa aatcttctac aaattgttct
     3421 tgcactttag cacgtcgtaa atattcattt ttcggatgaa tatttgaatt cgcgaaataa
     3481 attgcaatgt ctgcatattg tgttaaaaac tctaatgtat atgtactaca aggtgcacaa
     3541 caactatgca ataagatttt aggtctgatt gcttctcttt cccactgccc gattaatttc
     3601 ttcaacacct tgtcataatt aattttttga tttttcattt tactgataat aggttcagca
     3661 ttaatcatga tttaacctcc cacatttaat cattaacttc tattatatat gattcatatt
     3721 aaatgtcagt caaaaaagtt agaaattcat tttaatgcat ttatatttcg aaagtccctt
     3781 atgtatctaa aagcattttt taaacttgaa ttttaaaaca ctaaacaaca catacgtctc
     3841 tgtgtcattt tcattttttg tatgtcatat atatgtttac ttcatttaaa tcaatttcat
     3901 cttataattt atcgtgtatt ttacaaaaga ttgactccaa ttcatcataa aagttatact
     3961 tttgccattt tttaatgtaa catggtgtta gtaataaaaa taatacattg aggtgtttta
     4021 catgacagca ttattccctt atatcgcttt taaaaattca aaagaagccc ttgcatatta
     4081 cgaagaagta tttggtgcaa ctgacgttaa acgtttagaa gttggcgaag aacaagcgtc
     4141 acattttggt atgactaagg aagaagcgca agaagcaact atgcatgctg agtttgaagt
     4201 gcttggcgta aaagtgttat gttctgattc ttttggtcgc gctgacaaaa ttaataatgg
     4261 catatcatta ttaattgatt atgatgttaa caataaggaa gatgctgata aagttgaagc
     4321 attctatgag caaattaaag atcattcttc aattgaaata gaattaccgt tagctgacca
     4381 attctggggt ggcaaaatgg gcatctttac cgataaatac ggtgttcgtt ggatgttaca
     4441 tggtcaagac tatactgcaa tccaacaata atgttattga taaagatatc atttaataag
     4501 cttaggttat ttagcaaggc aagccactct cgattcatgg cttgccttat tttattgtat
     4561 atttttaatt atcgttgctg gcccttgatt gacaatcgta ttaaaatgcc ttattaagtc
     4621 aactttgtct atacggtttg gatcttctac ccaatgtctt atcaaagaca atcccgcacc
     4681 tgaaacataa ctcatgaaat aggaaaatgg tataccatta atttgatcat tttcattttt
     4741 ataacgttgt aaatgacaca taattaaatc ataaagtttt tcgtggatat ttgaatcttt
     4801 acccatacga aacatcaatt gataaaatgc gatgtctttt tctatcattt ctattaaaac
     4861 ggtcataatt tgatgtatat tatccgtgga taacttaact gctccattta acttctcatc
     4921 atgaatgaag tctcttattt cctccaactg ctgattctct aatttttcta gcaaatcata
     4981 tttatcataa taatgcgtat aaaatgtact acggttaaca tcagctaaat ctgcaatttg
     5041 ttgcacagta atctcttcta attggtgttg atgtaaaagt tcaataaata catttctcat
     5101 tgcaacttgt gattttctaa tacgtcgatc tatagtcatt tgtatcaagt cctccccaat
     5161 gattattaac aattataaac gttatgttca ttatcccaca aatctccaac attgatgatt
     5221 ggcacacaat gtttacctgt ttaatatagg tgatacaaac aaacagaaaa aggtgataac
     5281 aatgaaccaa catttactag gaaatccaaa attaactgta actcatgtca atgaagtgaa
     5341 agccggtatt aaccacatcg ttgtcgacag tgttcaatat ggaaatcaag aaatgattat
     5401 ggaaaaagat gtcactgtgg aaatgcgcga tggcgaaaaa ttatatatta atattttcag
     5461 accaaataaa gatggcaaat tccctgtagt tatgtctgca gatacttacg gtaaagataa
     5521 taagcctaaa atcacaaata tgggtgccct ttggccaaca ttaggcgcca ttccgacatc
     5581 tagttttaca cctgaagaat caccagaccc aggattttgg gtgccaaatg attatgttgt
     5641 agttaaagtt gcattacgcg gtagtgacaa atcaaaaggc gtcttatctc catggtcaaa
     5701 aagagaagcg gaagattatt acgaagtgat tgaatgggca gcaaaacagc catggagtaa
     5761 tggaaatatc ggaacaaatg gtgtttctta tcttgcggtg actcaatggt gggtcgcatc
     5821 attaaatcca ccacatttaa aagcaatgat tccttgggaa ggattaaatg atatgtatag
     5881 agaagtagcc tttcacggag gtataccgga tactggcttt tatcgtttct ggatacaagg
     5941 tatttttgcg agatggacag ataatccaaa tatcgaagac atgattcaag cacaacaaga
     6001 acaccctttg ttcgatgatt tttggaaaca gcgtcaagtg ccattatcac aaattaaaac
     6061 acctctacta acatgtgcta gttggtctac acaaggtttg cacaaccgtg gctcttttga
     6121 aggatttaaa caagctgcat ctgaagaaaa atggctatat gtgcatggac gtaaagagtg
     6181 ggaaagttac tacgctagag aaaatctcga acgccaaaaa tcattctttg atttttacct
     6241 taaagaagaa aataacgatt ggaaagatac gcctcatgtc atttatgaag ttagagatca
     6301 attttataaa ggcgaattca aatcagcgtc agcgttccct ttacctaaca cagaatatac
     6361 accattgtat ttgaatgctg aaaatcacac attgaatcat gcaaagatta gtagcgcgca
     6421 tgtcgcacaa tatgactctg aagataaacg acaagatgta agttttaaat atacgtttga
     6481 caaagatact gagttagttg gaaacatgaa cttaaaacta tgggtaagca ctaaagactc
     6541 agacgatatg gatttatttg caggtattaa aaagttagat cgtcgtggta atgaagttaa
     6601 cttccctgat tttaatcata ttgaaaatgg tcaagtagct accggttggt tacgcgtatc
     6661 acatcgtgaa ttagatcaag aaaaatccac aattgcgcaa ccttggcata aacatgaaac
     6721 agaattaaag ttgtcacaag atgagattgt acctgttgaa atcgaattgt taccttcagg
     6781 cacgctattt aaacaaggcg aaacattgga agttgttgta aaaggtagtg aaattgtaat
     6841 tggtaatagt acccctggca tgaaaacgcg ttatgaacat gaagaaaccg taaataaagg
     6901 catgcacatg atttatactg gtggtaaata tgattcacaa ttaatcattc ctatcgttaa
     6961 ttgatatgca gcaattacgg tcgcttttga ttaaaagtga catagtgata gaactgtata
     7021 acaagagcag gccacacgct tggaatctta aaccaaggtg tggccctttt tattattgat
     7081 ggctatttaa ttttataaat cttgtcacta aacttactac aagcaatatt aaaccaataa
     7141 ataaaaccat aattgctgta ttactttcgt tatttactcc tgtttttggt aattctttca
     7201 attgttgctt atgtactgac ttgctcttgc tatccaaatg aatactttgt tgtttagcca
     7261 tttgtttatt actttttgtg tgaatatcat ttgctttaat acttggattt acagcactat
     7321 tattcgatgt tccatttgcg tttttcaact gattaataac atttggatct ttcgtgccat
     7381 ccattaaatt atctggatgt tttagtaaat gtctgcagtc ggatactatt ttagaatctt
     7441 ttgcctttac cgcattattc ggcacctcat tatcatcttt caactgcttg ataacatctg
     7501 gatctttcgt gccatctatt aaattatcaa gttttttcac acgtgctatg tcactaactg
     7561 gccctggata atttgcatcc ggttgtctaa ctggtattaa attgctgttt tcattcacac
     7621 cttcaggctt gttattttcg gcgttcgcat tatccgtaaa aatcaacact gataaaattg
     7681 caacaaataa acttataact tttttcatct tattcatcct ttcctatttt actaccttaa
     7741 tatcattgct attaaagtaa cccatccatc tatttttttc gtcgtataaa gagtaatatt
     7801 tatacccatt tggaatattg taatagagtt tagctgtgta cgctttatct tttttaatta
     7861 atttattatc accctttttt gtaatttcaa tgtttttcca atatatatac ttacttttaa
     7921 ttactttcac tttcttatta tatggatgac ttctgtactt acctatccaa taatttatgt
     7981 aatctaattt taaatcattt attctcacgc ctgaattatg attatatctg ccgtatgcat
     8041 gtactccgat gatattgttt tttgaatctc ttatcgcact accactttgt cctgaaaatg
     8101 tgtctaccgt ataatacatt tcattctcat caaaactttc taatttccct ttacctttat
     8161 attgtctgtt gtctgacttg tctcctggaa atccagaaat ttcaatattt tcacctttgc
     8221 taatgcgtgt gttcaaagtt aagtaacctg ttttatttcc taaattacta tttaacttaa
     8281 taattgctat atcttctttt gtaggagcat tattaatcca agatttagat acaaaaaact
     8341 tatgcgaaaa ggcctttcct attgtatatg tttgcccatt tactccagca tagacatcaa
     8401 tctttttagc ccaaccctta tcgtctttag aatagacatt atgagccgct gtcaatacaa
     8461 tgtccttacc aatcatagta ccggtgcctt tatatacacg gttatttgaa aacgtcatat
     8521 ttaataatac agtctgctta taaggtcttt gtaaaatatt attaacaacc gttctttgat
     8581 ctttaccgaa aactcgctca gttaagggcg tactataatc acctacttca taaagctcat
     8641 tttgattacg ttcaattgtt cctgattcaa aattattatc actaccattt tctgaattga
     8701 cgataggatt attatcagct aagacaaaac tttcaccata ataactagaa acaattacta
     8761 ctaacaaaat caaaaatgat attttaaaaa tttttgtcat aggcattcct cctttaatct
     8821 atattatttt tatctatttc atataatatt attatataat tgctatcaat tcaatatttt
     8881 tacttcacga aactaaacaa catgtcatta acttatctcg ttaagttaag aattctattt
     8941 tctaccaaat aaattctgaa tactgtttta gataatcaat tcgcaaaact atttctttat
     9001 aaatatgtcc atcatttaac actgataaaa ttgcaacgat aataccttta cttctattac
     9061 gctaacccaa ttggcataaa aataaaaacc cctaatggat aactacattg ttatctacta
     9121 gaggtttcta acttatacta ttttataaca ctatcgtatt ttctttttca tgaatcattt
     9181 caataatgac attgtcttca ttcattactg ctactttagg tgcatggttt ttaatttctt
     9241 cttcattcaa ctgtgcataa gtcatgatta tgactacatc gcctacttca acaagtcttg
     9301 atgctgcacc gttcaaacaa attttaccac tacctctttc accagctatt acgtatgttt
     9361 caaaacgtgc accattatta ttattcacga tggctacttt ttcatttggc aagatgtcta
     9421 ccgcttccaa aatatctgaa tcaatcgtaa tactacctac ataatttaaa tttgactcag
     9481 tcactcttgc tctatgaatt ttagcattca tcattgttct tatcacttta ttcagctcca
     9541 attattatat tatctattaa acgcgctttt gaaaatttga cagctaacga gataaatatg
     9601 cgtccagtta tttcgtgttg ttctactaat tgaggataac tataaacagc aacttcttca
     9661 atgcgtccac ttatatgtga ttcaagatat tcagtaaccc tgtctataat tactttactt
     9721 tgacgttcac cgtcttgata caacgcttgt gctaatagca aacttttact taaatgtacc
     9781 gcttcttgtc gttcttgctc cgttaaataa acatttcttg aacttttcgc caaaccatct
     9841 gcttctcgaa cgatatcaat accaataatt tcaacggcat gattgaagtc ttttaccatt
     9901 tgctcgacaa tagccaattg ctgggcatct tttttaccaa aataagcata atccggcata
     9961 acaatattaa atagcttatt aactactgtt accaccccat caaaatgccc tggacgcttc
    10021 gctccttcta acacatcagc taatggacct actttgacat caatacctaa ttcacctgga
    10081 tacatatctt ctactgcagg atgaaaaaca atgtccgccc ctacttctga tactaattct
    10141 aaatctttat caatttgtct cggataagca tcgaaatctt cgtttggacc aaattgtaat
    10201 ggattaacaa atacactcac aactgtaata tcatttgtac taactgattc gcgtaccatc
    10261 gttaaatgtc catcatgtaa ggcacccatt gttgggataa aaccaatcgt tgttcctgag
    10321 cgtttggctg ctttaacaat gtgttgcatc tcttttaccg tagtaatcag cttagtcatt
    10381 gttattaacc tcattcataa ttttcttttt atacgtatat tcttctgatg gaaatgcacc
    10441 agatttaact tcttgatcgt attgttttaa accatccaca ccaacactaa aatcagcaaa
    10501 ttgcttcaca aatttcgctt tatgttcaac accataattt aacatatcgt gataaaccaa
    10561 tacttgacca tctgtacctt ttcctgcacc aataccaatg actggaattg ttaagtgctt
    10621 gctaatttct tctgctaaat catttggaat tgcttcaagt actaacgcaa ctgcaccagc
    10681 ttgttctaca tttttcgcat ctaaaataag ttgctccgct gcttctttcg ttgcaccttg
    10741 taatttatac cccataacgc caacactttg aggtgttaat cctaaatgtg caacaacagg
    10801 aataccaatt gccgttgctt tttcaataaa tggtgtaata tgcgctcctt ctgctttaat
    10861 tgcatttgca ttcgtctcct gataaagctt tagagcatga tttaagtctt gtgtcataga
    10921 gatgcctact gcaccaatcg gcatatcaac aactacaaat gtatttggtg cgcctcttct
    10981 tactgcacga ccgtgatgaa tcatatctgc taacgtcact tgtacggtac tttcataacc
    11041 taatacagtc ataccaagtg aatccccaac aagaatcata tcaatacccg ctgcttccac
    11101 ttgtttagca cttggaaaat cataagctgt taccatagaa attttagttt gcttttgttt
    11161 catatctatt aattgactta ctgttttcaa tgttattcaa cctctttttg cagtattatt
    11221 agaaatagta taatgaatct tttatagaaa ggaaagaaga agatgttatc agttgcgatt
    11281 atcggcccag gcgctgtcgg tacaactatt gcctatgaat tacaacaatc attgcctcat
    11341 acaacgctta tcgggagaca cgccaaaaca ataacatatt atactgtacc acatgcacct
    11401 gcacaagata ttgttgtgaa aggttatgaa gatgtcacaa acacatttga tgttattatt
    11461 attgcagtca aaacacatca acttgatgca gttattcctc atttaactca tcttgcacat
    11521 gaggacacgc tcatcatttt agcccaaaat ggttatggtc aactcgaaca tattccattt
    11581 aaaaacatat gccaagcagt tgtctatata agtggtcaaa agaaaggcga tgttgttacg
    11641 cacttcagag attatcaatt acgtataaaa gataatgcat taacacgaca atttagagat
    11701 ttggtccaag acagtcaaat cgacattgta ttagaagcaa atattcagca agctatttgg
    11761 tataaattgt tagttaactt gggcattaat tctatcacag cactcggtag acagacagtt
    11821 gcaatcatgc ataatcctga aatacgtaca ctgtgtagac agctattact agatggttgt
    11881 cgtgttgcac aggcagaggg gttaaacttt tcagaacaaa ctgtcgatac tattatgact
    11941 atatatcaag gttatcccga cgaaatggga acgagcatgt attacgacat tgtgcatcag
    12001 caacctcttg aagtcgaagc gattcaaggc tttatttatc gacgtgcacg tgaacacaat
    12061 cttgacaccc catatcttga tactatttat agctttttac gcgcgtatca acaaaatatg
    12121 taaccacata tataatataa aaagacctta acgatgcatt tagaaccacc tgttcctaat
    12181 atcgctaagg tcttttaatt atattagact attcagcttc tctaatttct tctgccacat
    12241 ctttatagtc tattttcgct ttaacaaacg tctcgttatt taccgggaaa tgttgttgga
    12301 atgtttcaaa gttttgtatc tcaacgacaa cgtcatccac ttcaaagtca agaacatgtc
    12361 caccataagc tctttcatca tccgcaaaat gtatatgaaa accagcagac cctacgccat
    12421 gaaataattc tggtgtaaaa aatccaacaa tggcaccacg aatatcttgt cttttttcct
    12481 caggttgtct gcgtgctgaa tcaatcaaac gtgtatatgg cggttgctga gcagacatca
    12541 ttcgtacatg catatgttta aatgtgccat aaattttaac agccgaaaat aaattctcac
    12601 ttaacatttc atttttaatt tgtgcgaata catcatcttg tgataattgt tgcaatggaa
    12661 atgtcttact cgctttaaaa ttagtaatcg atgcataggg tactttctca tcgcctttta
    12721 attctataaa ttctttatgc tcgttagcat gatatgcctt cccgtctaaa aatattactt
    12781 cgccatcaga ccctgttaac gttgcaatcc ctaaattccc atgttctaac aattcattaa
    12841 ttgtagctgt tccttctagt aagccagcca ttaacgtacc taatgtacca tgttgataca
    12901 agacattcgt cattccaaaa ccatctccct atagttattc ctcgttgtca tgctctactt
    12961 cgtgattaat gcagaactac tttcctttat actcaatgac gtctcggcat ttcgattcat
    13021 gtcactaaat tttcacgagt cataatactt tattcaatta ttattttacg caaaagtatt
    13081 gcctctatca atgcttgcta aatcaaaacc acccgtgtga acgggtggtt tgttctgcgg
    13141 ctataagcct tccttactgg ccagccctaa aagggcactg acaagtcagc caactgcact
    13201 actattccag caaccctaaa gggttactct tttttcttt
//
LOCUS       DAHHOJ010000075        12698 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.075, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000075 DAHHOJ010000000
VERSION     DAHHOJ010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12698)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12698)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12698
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.075"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            148..1044
                     /gene="xerC"
                     /locus_tag="NBH32_001823"
     CDS             148..1044
                     /gene="xerC"
                     /locus_tag="NBH32_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCD1515416.1"
                     /translation="MNHIQEAFLNTLKVERNFSEHTLKSYQDDLIQFNQFLEQEHLQL
                     NTFEYRDARNYLSYLYSNHLKRTSVSRKISTLRTFYEYWMTLDENIINPFVQLVHPKK
                     EKYLPQFFYEEEMEALFKTVEEDTSKSLRDRVILELLYATGIRVSELVNIKKQDIDFY
                     ANGVTVLGKGSKERFVPFGAYCRQSIENYLEHFKPIQSCNHDFLIVNMKGEAITERGV
                     RYVLNDIVKRTAGVSEIHPHKLRHTFATHLLNQGADLRTVQSLLGHVNLSTTGKYTHV
                     SNQQLRKVYLNAHPRAKKENET"
     gene            1041..1586
                     /gene="hslV"
                     /locus_tag="NBH32_001824"
     CDS             1041..1586
                     /gene="hslV"
                     /locus_tag="NBH32_001824"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCD1515417.1"
                     /translation="MSNTTLHATTIYAVRHNGKAAMAGDGQVTLGQQVIMKQTARKVR
                     RLYEDKVLAGFAGSVADAFTLFEKFETKLQQFSGNLERAAVELAQEWRGDKQLRQLEA
                     MLIVMDKDAILVVSGTGEVIAPDDDLIAIGSGGNYALSAGRALKRHASHLSAEEMAYE
                     SLKVAADICVFTNDNIVVETL"
     gene            1652..3055
                     /gene="hslU"
                     /locus_tag="NBH32_001825"
     CDS             1652..3055
                     /gene="hslU"
                     /locus_tag="NBH32_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HCD1515418.1"
                     /translation="MDTAGIRLTPKEIVSKLNEYIVGQNDAKRKVAIALRNRYRRSLL
                     DEESKQEISPKNILMIGPTGVGKTEIARRMAKVVGAPFIKVEATKFTEVGYVGRDVES
                     MVRDLVDVSVRLVKAQKKSLVQDEATAKANEKLVKLLVPSMKKKASQTNNPLESLFGG
                     AIPNFGQNNEDEEEPPTEEIKTKRSEIKRQLEEGKLEKEKVRIKVEQDPGALGMLGTN
                     QNQQMQEMMNQLMPKKKVEREVAVETARKILADSYADELIDQESANQEALELAEQMGI
                     IFIDEIDKVATNNHNSGQDVSRQGVQRDILPILEGSVIQTKYGTVNTEHMLFIGAGAF
                     HVSKPSDLIPELQGRFPIRVELDSLSVEDFVRILTEPKLSLIKQYEALLQTEEVTVNF
                     TDEAITRLAEIAYQVNQDTDNIGARRLHTILEKMLEDLSFEAPSMPNAVVDITPQYVD
                     DKLKSISTNKDLSAFIL"
     gene            3080..3853
                     /gene="codY"
                     /locus_tag="NBH32_001826"
     CDS             3080..3853
                     /gene="codY"
                     /locus_tag="NBH32_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HCD1515419.1"
                     /translation="MSLLSKTRELNTLLQKHKGIAVDFKDVAQTISSVTVTNVFIVSR
                     RGKILGSSLNELLKSQRIIQMLEERHIPSEYTERLMEVKQTESNIDIDNVLTVFPPEN
                     RELFIDSRTTIFPILGGGERLGTLVLGRVHDDFNENDLVLGEYAATVIGMEILREKHS
                     EVEKEARDKAAITMAINSLSYSEKEAIEHIFEELGGTEGLLIASKVADRVGITRSVIV
                     NALRKLESAGVIESRSLGMKGTFIKVKKEKFLDELEKSK"
     gene            3904..3996
                     /locus_tag="NBH32_001827"
     CDS             3904..3996
                     /locus_tag="NBH32_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515420.1"
                     /translation="MAVVNEMTLMIMLKSFLYFCLIFNQIDIPH"
     gene            4195..4962
                     /gene="rpsB"
                     /locus_tag="NBH32_001828"
     CDS             4195..4962
                     /gene="rpsB"
                     /locus_tag="NBH32_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCD1515421.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKKVDEAYNFLKQVSEDGGQVLFVGTKKQAQESVKSEAERAGQFYINQRWLGGLLT
                     NYKTISKRIKRISEIEKMEEDGLFEVLPKKEVVELKKEYDRLIKFLGGIRDMKSMPQA
                     LFVVDPRKERNAIAEARKLNIPIVGIVDTNCDPDEIDYVIPANDDAIRAVKLLTAKMA
                     DAILEGQQGVSNEEVAAEQNIDLDEKEKSEETEATEE"
     gene            4996..5109
                     /locus_tag="NBH32_001829"
     CDS             4996..5109
                     /locus_tag="NBH32_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515422.1"
                     /translation="MGDKILMLITFFKKKIEANYKYSIRVLAILPIIMLKS"
     gene            5144..6025
                     /locus_tag="NBH32_001830"
     CDS             5144..6025
                     /locus_tag="NBH32_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCD1515423.1"
                     /translation="MATISAKLVKELREKTGAGMMDCKKALTETDGDIDKAIDYLREK
                     GIAKAAKKADRIAAEGLVHVETKGNDAVIVEINSETDFVARNEGFQELVKEIANQVLD
                     TKAETVEALMETTLPNGKSVDERIKEAISTIGEKLSVRRFAIRTKTDNDAFGAYLHMG
                     GRIGVLTVVEGSTDEEAARDVAMHIAAINPKYVSSEQVSEEEINHEREVLKQQALNEG
                     KPENIVEKMVEGRLRKYLQEICAVDQDFVKNPDVTVEAFLKTKGGKLVDFVRYEVGEG
                     MEKREENFADEVKGQMK"
     gene            6162..6884
                     /locus_tag="NBH32_001831"
     CDS             6162..6884
                     /locus_tag="NBH32_001831"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000057332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCD1515424.1"
                     /translation="MAQISKYKRVVLKLSGEALAGEKGFGINPVIIKSVAEQVAEVAK
                     MDCEIAVIVGGGNIWRGKTGSDLGMDRGTADYMGMLATVMNALALQDSLEQLDCDTRV
                     LTSIEMKQVAEPYIRRRAIRHLEKKRVVIFAAGIGNPYFSTDTTAALRAAEVEADVIL
                     MGKNNVDGVYSADPKVNKDAVKYEHLTHIQMLQEGLQVMDSTASSFCMDNNIPLTVFS
                     IMEERNIKRAVMGEKIGTLITK"
     gene            6903..7457
                     /gene="frr"
                     /locus_tag="NBH32_001832"
     CDS             6903..7457
                     /gene="frr"
                     /locus_tag="NBH32_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCD1515425.1"
                     /translation="MSDIINETKSRMQKSIESLSRELANISAGRANSNLLNGVTVDYY
                     GAPTPVQQLASINVPEARLLVISPYDKTSVADIEKAIIAANLGVNPTSDGEVIRIAVP
                     ALTEERRKERVKDVKKIGEEAKVSVRNIRRDMNDQLKKDEKNGDITEDELRSGTEDVQ
                     EATDNSIKEIDQMIADKEKDIMSV"
     gene            7830..8600
                     /locus_tag="NBH32_001833"
     CDS             7830..8600
                     /locus_tag="NBH32_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HCD1515426.1"
                     /translation="MFKKLINKKNTINNYNEELDSSNIPEHIAIIMDGNGRWAKKRKM
                     PRIKGHYEGMQTIKKITRIASDIGVKYLTLYAFSTENWSRPESEVNYIMNLPVNFLKT
                     FLPELIEKNVKVETIGFTDKLPKSTIEAINNAKEKTANNTGLKLIFAINYGGRAELVH
                     SIKNMFDELHQQGLNSDIIDETYINNHLMTKDYPDPELLIRTSGEQRISNFLIWQVSY
                     SEFIFNQKLWPDFDEDELIKCIKIYQSRQRRFGGLSEE"
     gene            8607..9389
                     /locus_tag="NBH32_001834"
     CDS             8607..9389
                     /locus_tag="NBH32_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCD1515427.1"
                     /translation="MKVRTLTAIIALIVFLPILLKGGLVLMIFANILALIALKELLNM
                     NMIKFVSVPGLISAVGLIIIMLPQHAGPWVQVIQLKSLIAMSFIVLSYTVLSKNRFSF
                     MDAAFCLMSVAYVGIGFMFFYETRSEGLHYILYAFLIVWLTDTGAYLFGKMMGKHKLW
                     PVISPNKTIEGFIGGLFCSLIVPLAMLYFVDFNMNVWILLGVTLILSLFGQLGDLVES
                     GFKRHFGVKDSGRILPGHGGILDRFDSFMFVLPLLNILLIQA"
     gene            9601..10887
                     /gene="rseP"
                     /locus_tag="NBH32_001835"
     CDS             9601..10887
                     /gene="rseP"
                     /locus_tag="NBH32_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000121122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HCD1515428.1"
                     /translation="MSYLVTIIAFIIVFGVLVTVHEYGHMFFAKRAGIMCPEFAIGMG
                     PKIFSFRKNETLYTIRLLPVGGYVRMAGDGLEEPPVEPGMNVKIKLNEENEITHIILD
                     DHHKFQQIEAIEVKKCDFKDDLFIEGITAYDNERHHFKIARKSFFVENGSLVQIAPRD
                     RQFAHKKPWPKFLTLFAGPLFNFILALVLFIGLAYYQGTPTSTVEQVADKYPAQQAGL
                     QKGDKIVQIGKYKISEFDDVDKALDKVKDNKTTVKFERDGKTKSVELTPKKTERKLTK
                     VSSETKYVLGFQPASEHTLFKPIVFGFKSFLIGSTYIFTAVVGMLASIFTGGFSFDML
                     NGPVGIYHNVDSVVKAGIISLIGYTALLSVNLGIMNLIPIPALDGGRILFVIYEAIFR
                     KPVNKKAETTIIAIGAIFMVVIMILVTWNDIRRYFL"
     gene            10907..12610
                     /locus_tag="NBH32_001836"
     CDS             10907..12610
                     /locus_tag="NBH32_001836"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCD1515429.1"
                     /translation="MKQSKVFIPTMRDVPSEAEAQSHRLLLKSGLIKQSTSGIYSYLP
                     LATRVLNNITAIVRQEMERIDSVEILMPALQQAELWEESGRWGAYGPELMRLQDRHGR
                     QFALGPTHEELVTSIVRNELKSYKQLPMTLFQIQSKFRDEKRPRFGLLRGREFIMKDA
                     YSFHADEASLDQTYQDMYQAYSRIFERVGINARPVVADSGAIGGSHTHEFMALSAIGE
                     DTIVYSKESDYAANIEKAEVVYEPNHKHTTVQPIEKIETPNVKTAQELADFLGRPVDE
                     IVKTMIFKVDGEYIMVLVRGHHEINDIKLKSYFGTDNIELATQDEIVNLVGANPGSLG
                     PVIDKEIKIYADNFVQDLNNLVVGANEDGYHLINVNVGRDFNVDAYGDFRFILEGEKL
                     SDGSGVAHFAEGIEVGQVFKLGTKYSESMNATFLDNQGKAQPLIMGCYGIGISRTLSA
                     IVEQNHDDNGIVWPKSVTPFDLHLISINPKKDDQRELADALYAEFNTKFDVLYDDRQE
                     RAGVKFNDADLIGLPLRIVVGKRASEGIVEVKERLTGDSEEVHIDDLITVITNKYDNL
                     K"
BASE COUNT         4640 a         1731 c         2495 g         3832 t
ORIGIN      
        1 attaatttaa aaaagaggta ttctaattaa caggttttcc gattagttac aattatttaa
       61 ttctcaaaag atttagaatt gattatcaaa ttactgtaag ccctttgctg tatatgctac
      121 aattcttatt gatggagggt aaatgtattg aatcatattc aagaagcgtt tttaaataca
      181 ttgaaagttg aacggaattt ttcggaacat acattgaaat catatcaaga tgacttaatt
      241 cagtttaatc aatttttaga acaagaacat ttacagttga atacttttga atacagagat
      301 gctagaaatt atttgagcta tttatattca aatcatttga aaagaacatc tgtttctcgt
      361 aaaatctcaa cgttaagaac tttctatgaa tattggatga cgcttgatga gaacattatt
      421 aatccatttg ttcaattagt acatccgaaa aaagaaaaat atcttccgca attcttttac
      481 gaagaagaaa tggaagcgtt atttaaaact gtagaagagg acacttcaaa aagtttacgg
      541 gatcgagtta ttcttgaatt gttatatgct acaggtatcc gtgtttcgga attagttaat
      601 attaaaaaac aagatataga tttttacgcg aatggtgtta ccgtgttagg aaaagggagc
      661 aaagagcgct ttgtaccgtt tggtgcttat tgtagacaaa gcatcgaaaa ttatttagaa
      721 catttcaaac ctattcagtc atgcaatcat gattttctta ttgtaaatat gaagggtgaa
      781 gcaatcactg aacgcggtgt acgatatgtt ttaaatgata ttgttaaacg aacagcaggc
      841 gtaagtgaga ttcatcccca caagctcaga catacatttg caacgcattt attgaatcaa
      901 ggtgcagacc taagaacagt acaatcatta ttaggtcatg ttaatttgtc aacaactggt
      961 aaatatacac acgtatctaa ccaacaatta agaaaagtgt atctaaatgc acatcctcga
     1021 gcgaaaaagg agaatgaaac atgagtaata caacattaca tgcaacaaca atttatgctg
     1081 taagacataa tgggaaagca gctatggctg gagatgggca agtaacgctt ggtcaacaag
     1141 tcatcatgaa acaaacggca agaaaagtgc gacgtttata tgaagataaa gtgttagctg
     1201 gtttcgcagg tagtgtagca gatgcgttta cgttatttga aaaattcgaa acaaaattac
     1261 aacagtttag tggtaactta gaaagagctg ctgttgaatt ggcacaagaa tggcgaggcg
     1321 ataaacaatt acgtcaatta gaagctatgc taattgtaat ggataaagat gctattttag
     1381 ttgtcagtgg aactggcgaa gttattgccc cagatgatga ccttatcgct attggatcag
     1441 gaggcaacta cgcattaagc gcaggacgtg cattgaaacg ccatgcatcg catttgtctg
     1501 ctgaagaaat ggcatatgag agcttgaaag tagcggctga tatttgtgtc tttaccaacg
     1561 ataatattgt tgtcgaaaca ctataataat cagagcacga taaataatta cgagcaatta
     1621 attttagtta aaagacggag gaatgaaatt aatggataca gctggaataa gattaactcc
     1681 aaaagaaatc gtatctaaat taaatgaata catcgttgga caaaatgatg ctaaacgtaa
     1741 agtggcaatt gccctacgta atcgatacag aagaagttta ttagatgagg aatcaaagca
     1801 agaaatttca cctaaaaata ttttgatgat tggaccaacc ggcgttggta aaactgaaat
     1861 tgcaagaaga atggccaaag ttgtcggcgc accatttata aaagtagaag ctactaaatt
     1921 tactgaggta ggttatgtag gacgagatgt tgaaagtatg gttagagatc ttgttgatgt
     1981 ttcagtaagg ttagtcaagg cgcagaaaaa atcattggta caagatgaag caacagctaa
     2041 ggctaatgaa aaacttgtta agttattagt tccaagtatg aaaaagaaag catctcaaac
     2101 gaataatcct ttagagtcac ttttcggagg tgcaattcca aatttcggac aaaataacga
     2161 agatgaagaa gaaccaccta ctgaggaaat taaaacaaaa cgttctgaaa ttaagagaca
     2221 attagaagaa ggcaaacttg aaaaagaaaa ggtaagaatt aaagtcgaac aagatcctgg
     2281 tgctttaggt atgctaggta caaatcaaaa tcagcaaatg caagagatga tgaatcaatt
     2341 aatgcctaaa aagaaagttg agcgagaagt tgctgttgag acggcaagga aaatactagc
     2401 tgatagttat gcggatgaac taattgatca agaaagcgct aaccaagaag cacttgaatt
     2461 agcagaacaa atgggtatca tctttataga tgaaatcgac aaagttgcga cgaataatca
     2521 taatagtggt caagatgtct caagacaagg tgttcaaaga gatattttac ctatacttga
     2581 aggtagcgtt attcaaacca aatatggtac tgtgaatact gaacatatgc tgtttatagg
     2641 tgctggagct ttccatgtat ctaagccgag tgacttgata ccagaattgc aaggtcgttt
     2701 tccgattaga gttgaacttg atagtttatc ggtagaagat tttgtaagaa ttttgacaga
     2761 accaaaattg tcattaatta aacaatatga agcattgctt caaacagaag aagttactgt
     2821 aaactttacc gatgaagcaa ttactcgctt agctgagatt gcctatcaag taaatcaaga
     2881 tacagacaat attggtgcac gtcgacttca tacaatttta gaaaagatgc tagaagattt
     2941 atcattcgaa gcaccaagta tgccgaatgc agttgtagat attaccccac aatatgttga
     3001 tgataaatta aaatcaattt caacaaataa agatttaagt gcatttattc tataaaaata
     3061 tacaaaagga gaaaaattca tgagcttatt atctaaaacg agagagttaa acacgttact
     3121 tcaaaaacac aaaggtattg cggttgattt taaagatgta gcacaaacga ttagtagcgt
     3181 aactgtaaca aatgtattta ttgtatcgcg tcgaggtaaa attttaggat cgagtctaaa
     3241 tgaattatta aaaagtcaaa gaattattca aatgttggaa gaaagacata ttccaagtga
     3301 atatacagaa cgattaatgg aagttaaaca aacagaatca aatattgata tcgacaatgt
     3361 attaacagtt ttcccacctg aaaacagaga attattcata gatagtcgta caactatctt
     3421 cccaatttta ggtggaggag aaagattagg tacattagta cttggtcgag tacatgatga
     3481 ttttaatgaa aatgatttgg tactaggtga atatgctgct acagttattg gtatggaaat
     3541 cttacgtgag aagcatagtg aagtagaaaa agaagcgcgc gataaagctg ctattacaat
     3601 ggcaattaat tcattatctt attctgaaaa agaagcaatt gaacatatct ttgaagaact
     3661 tggcggtacg gaaggcctat taatcgcatc aaaagttgca gatagagttg gtattactag
     3721 atctgtaatt gtaaatgcac tacgtaaatt agaaagtgct ggtgtaattg aatcacgttc
     3781 tttaggaatg aaaggtactt tcattaaagt taaaaaagaa aaattcttag atgaattaga
     3841 aaaaagtaag taaaacgatg agtctggggc ataattcaat gtcctagtcg tgacgatgtt
     3901 atcttggcag tagtcaacga aatgactttg atgattatgt tgaagtcatt tctttatttt
     3961 tgtctaattt tcaatcaaat tgatattcca cattaacatg atagaattac ctttaaattt
     4021 atgaaaaaat gcttgaaaaa catttgcttt agacacggca ttatgttata tttattttcg
     4081 gctataaagc caatacacac atatcaaacg tgattgtgta aagggtgcaa tatttgtata
     4141 ttgtctttac aatagtttgc tatggaggta attaaccaat aggaggaatt tataatggca
     4201 gtaatttcaa tgaaacaatt actagaagcg ggtgttcact tcggtcacca aacacgtcgt
     4261 tggaacccaa aaatgaaaaa atatatcttc actgagagaa atggtattta tatcatcgac
     4321 ttacaaaaaa cagtgaaaaa agtagacgag gcatacaact tcttgaaaca agtttcagaa
     4381 gatggtggac aagtcttatt cgtaggaact aaaaaacaag cacaagaatc agttaaatct
     4441 gaagcagaac gtgctggtca attctacatt aaccaaagat ggttaggtgg attattaact
     4501 aactataaaa cgatctcaaa acgaatcaaa cgtatttctg aaattgaaaa aatggaagaa
     4561 gatggtttat tcgaagtatt acctaaaaaa gaagtagtag aacttaaaaa agaatatgac
     4621 cgtttaatca aattcttagg cggaattcgt gatatgaaat caatgcctca agcattattc
     4681 gtagttgacc cacgtaaaga gcgtaatgca attgctgaag ctcgtaaatt aaatattcct
     4741 atcgtaggta tcgttgacac taactgtgat cctgacgaaa ttgactacgt tatcccagca
     4801 aacgacgatg ctatccgtgc ggttaaatta ttaactgcta aaatggcaga tgcaatctta
     4861 gaaggtcaac aaggcgtttc taatgaagaa gtagctgcag aacaaaacat cgatttagat
     4921 gaaaaagaaa aatcagaaga aacagaagca actgaagaat aatcaactgt tgaatctgac
     4981 ttagatatag tttaaatggg tgataagata ttaatgctta tcaccttttt taaaaagaaa
     5041 atcgaggcaa attacaaata ttcaattaga gtattggcaa tcttgcctat aataatgcta
     5101 aaatcataat atataaaatg ataacttatt ggaggaataa tgaatggcaa ctatttcagc
     5161 aaaacttgtt aaagaattac gtgaaaaaac tggcgcgggt atgatggatt gtaaaaaagc
     5221 gctaactgaa actgatggtg acatcgataa agcgattgac tacctacgtg aaaaaggtat
     5281 tgctaaagca gctaaaaaag cagaccgtat tgcggctgaa ggtttagtac atgtagaaac
     5341 taaaggtaac gacgcagtta tcgttgaaat caactctgaa acagactttg ttgctcgtaa
     5401 cgaaggtttc caagagttag ttaaagaaat cgctaatcaa gtattagata caaaagctga
     5461 aactgttgaa gctttaatgg aaacaacttt accaaatggt aaatcagttg atgaaagaat
     5521 taaagaagca atttcaacaa tcggtgaaaa attaagtgtt cgtcgttttg ctatcagaac
     5581 taaaactgat aacgatgctt tcggcgctta cttacacatg ggtggacgca ttggtgtatt
     5641 aacagttgtt gaaggttcaa ctgacgaaga agcagcaaga gacgttgcta tgcatatcgc
     5701 tgcaatcaac cctaaatatg tttcttctga acaagttagc gaagaagaaa tcaaccacga
     5761 aagagaagtt ttaaaacaac aagcattaaa tgaaggtaaa ccagaaaaca tcgttgaaaa
     5821 aatggtggaa ggacgtttac gtaaatactt acaagaaatt tgtgctgtag atcaagactt
     5881 cgttaaaaac cctgatgtaa cagttgaagc tttcttaaaa acaaaaggtg gaaaacttgt
     5941 tgacttcgta cgctatgaag taggcgaagg tatggaaaaa cgcgaagaaa actttgcgga
     6001 tgaagttaaa ggacaaatga aataatctgt cataaagtaa aacaaggaag aagacacctt
     6061 taatgttgct ttattaaaat gtaaatcatt ctaataaaac gacaactgtg tcttctttac
     6121 ttgtatatgt tacatatatt cacgatagag aggataagaa aatggctcaa atttctaaat
     6181 ataaacgtgt agttttgaaa ctaagtggtg aagcgttagc tggagaaaaa ggatttggca
     6241 taaatccagt aattattaaa agtgttgctg agcaagtggc tgaagttgct aaaatggact
     6301 gtgaaatcgc agtaatcgtt ggtggcggaa acatttggag aggtaaaaca ggtagtgact
     6361 taggtatgga ccgtggaact gctgattaca tgggtatgct tgcaactgta atgaatgcct
     6421 tagcattaca agatagttta gaacaattgg attgtgatac acgagtatta acatctattg
     6481 aaatgaagca agtggctgaa ccttatattc gtcgtcgtgc aattagacac ttagaaaaga
     6541 aacgtgtagt tatttttgct gcaggtattg gaaacccata cttctctaca gatactacag
     6601 cggcattacg tgctgcagaa gttgaagcag atgttatttt aatgggcaaa aataatgtag
     6661 atggtgtata ttctgcagat cctaaagtaa acaaagatgc ggtaaaatat gaacatttaa
     6721 cgcatattca aatgcttcaa gaaggtttac aagtaatgga ttcaacagca tcctcattct
     6781 gtatggataa taacattccg ttaactgttt tctctattat ggaagaaaga aatattaaac
     6841 gtgctgttat gggtgaaaag ataggtacgt taattacaaa ataaatttag aggtgtaaaa
     6901 taatgagtga cattattaat gaaactaaat caagaatgca aaaatcaatc gaaagcttat
     6961 cacgtgaatt agctaatatc agtgcaggaa gagctaattc aaatttatta aacggcgtaa
     7021 cagttgatta ctatggtgca ccaacacctg tacaacaatt agcaagcatc aatgttccag
     7081 aagcacgttt acttgttatt tctccatacg acaaaacttc tgtagctgac atcgaaaaag
     7141 cgataatagc ggctaactta ggtgtcaacc caacaagtga tggtgaagtg atacgtattg
     7201 cagtacctgc cttaacagaa gagcgtagaa aagagcgcgt taaagatgtt aagaaaattg
     7261 gtgaagaagc taaagtatct gttcgaaata ttcgtcgtga tatgaatgat cagttgaaaa
     7321 aagatgaaaa aaatggcgac attactgaag atgagttgag aagtggtact gaagatgttc
     7381 aggaagcaac agacaattca ataaaagaaa ttgatcaaat gattgctgat aaagaaaaag
     7441 atattatgtc agtataaaac taatatacaa tgacatatta aaatgccagt attaaacgat
     7501 aatgtaacat ttaaaatggg catgtttaat taaatcaaag atgcatgtga taatttaaat
     7561 tcacaatgag cataaaaatg gtgtttaaac aagttaatta aacatatact ttataaataa
     7621 taggcattag gtatattgct ataataaagt tatgtaattt ttaacctcag tatgtatgtc
     7681 acatttctgg tgtaaactgt accgagtcag actttggtac agttttttta tttgcttatt
     7741 caatgcatta aatgagtatg ataaaatgat aatgattgtt tagtaactta tactatatga
     7801 cagagatgat caggctcgga ggaaagacca tgtttaaaaa gctaataaat aaaaagaaca
     7861 ctataaataa ttataatgaa gaattagact cgtctaatat acctgaacat atcgctatta
     7921 ttatggatgg taatgggcga tgggctaaga agcgaaaaat gcctagaatt aaaggccatt
     7981 acgaaggtat gcaaacaata aaaaaaatta ctaggatagc tagtgatatt ggtgttaagt
     8041 acttaacttt atatgccttt tccactgaaa attggtcaag acctgaaagt gaagtaaatt
     8101 atattatgaa tttgcctgtc aatttcttaa agacattctt accggaacta attgaaaaaa
     8161 atgtcaaagt tgaaacaatt ggatttactg ataagttgcc aaaatcaacg atagaagcaa
     8221 ttaataatgc taaagaaaag acagctaata ataccggctt aaaattaata tttgcaatta
     8281 attatggtgg cagagcagaa cttgttcata gtattaaaaa tatgtttgac gagcttcatc
     8341 aacaaggttt aaatagtgat atcatagatg aaacatatat aaacaatcat ttaatgacaa
     8401 aagactatcc tgatccagag ttgttaattc gtacttcagg agaacaaaga ataagtaatt
     8461 tcttgatttg gcaagtttcg tatagtgaat ttatctttaa tcaaaaatta tggcctgact
     8521 ttgacgaaga tgaattaatt aaatgtataa aaatttatca gtcacgtcaa agacgctttg
     8581 gcggattgag tgaggagtag tatagtatga aagttagaac gctgacagct attattgcct
     8641 taatcgtatt cttgcctatc ttgttaaaag gcggccttgt gttaatgata tttgctaata
     8701 tattagcatt gattgcatta aaagaattgt tgaatatgaa tatgattaaa tttgtttcag
     8761 ttcctggttt aattagtgca gttggtctta tcatcattat gttgccacaa catgcagggc
     8821 catgggtaca agtaattcaa ttaaaaagtt taattgcaat gagctttatt gtattaagtt
     8881 atactgtctt atctaaaaac agatttagtt ttatggatgc tgcattttgc ttaatgtctg
     8941 tggcttatgt aggcattggt tttatgttct tttatgaaac gagatcagaa ggattacatt
     9001 acatattata tgccttttta attgtttggc ttacagatac aggggcttac ttgtttggta
     9061 aaatgatggg taaacataag ctttggccag taataagtcc gaataaaaca atcgaaggat
     9121 tcataggtgg cttgttctgt agtttgatag taccacttgc aatgttatat tttgtagatt
     9181 tcaatatgaa tgtatggata ttacttggag tgacattgat tttaagttta tttggtcaat
     9241 taggtgattt agtggaatca ggatttaagc gtcatttcgg cgttaaagac tcaggtcgta
     9301 tactacctgg acacggtggt attttagacc gatttgacag ctttatgttt gtgttaccat
     9361 tattaaatat tttattaata caagcttaat gctgagaaca aatcaataaa cgtaaagagg
     9421 agttgctgag ataatttaat gaatctcaga actccttttg aaaattatac gcaatattaa
     9481 ctttgaaaat tagacgttat attttgtgat ttgtcagtat catattataa tgacttatgt
     9541 tacgtataca gcaatcaatt ttaaaataaa agaaatttat aaacaatcga ggtgtagcga
     9601 gtgagctatt tagttacaat aattgcattt attattgttt ttggtgtact agtaactgtt
     9661 catgaatatg gccatatgtt ttttgcgaaa agagcaggca ttatgtgtcc agaatttgcg
     9721 atcggtatgg ggccaaaaat ttttagtttt agaaaaaatg aaacacttta cactattagg
     9781 ttattgcctg ttggtggata tgttcgtatg gcaggagatg gcttagaaga gccaccagtc
     9841 gagcccggta tgaacgttaa aattaaactg aatgaagaaa atgaaataac acatatcata
     9901 ttagatgatc atcataagtt tcaacaaatt gaagcgatcg aagttaaaaa atgtgatttt
     9961 aaggatgact tattcataga aggtatcact gcttatgata atgaaagaca tcattttaaa
    10021 attgctagaa agtctttctt tgttgaaaat ggtagcttag ttcaaattgc tccgagagac
    10081 agacaatttg cacataaaaa gccatggccg aaatttttaa cattatttgc gggaccgtta
    10141 tttaacttta tattagcttt agtcctattt attggtcttg catattatca aggtacacct
    10201 acgtctactg tagaacaagt cgcagataag tatccagcac aacaagcagg attacaaaaa
    10261 ggtgataaaa ttgttcaaat tggcaaatat aaaatatctg aatttgatga tgttgataag
    10321 gcgttagata aagttaaaga taataagacg actgttaaat ttgaacgtga tggtaaaacg
    10381 aagtcagttg aattaacacc taaaaagact gaaagaaaac tgactaaagt aagttcagag
    10441 acgaagtatg ttctcggatt ccaaccagcg agtgaacata cactttttaa accaattgta
    10501 ttcggattta aaagcttttt aatcggtagt acttatattt ttacagctgt agtaggtatg
    10561 ttggctagta tatttacggg cggattctca tttgatatgt taaatggtcc ggttggtatt
    10621 tatcataacg tagactcagt tgttaaagcg ggtatcatta gcttaattgg ttacactgcg
    10681 ttattaagtg taaacttagg tattatgaat ttaattccta ttcctgcact agacggtggt
    10741 cgtattttat ttgttatata tgaagcgatt ttcagaaaac cagttaataa aaaagcggaa
    10801 acaacgatta ttgctattgg tgccattttc atggtcgtta ttatgatatt agtaacgtgg
    10861 aatgatattc gacgatattt cttataattt aggaggataa ataattatga agcaatccaa
    10921 agtttttata ccaacgatgc gtgacgtgcc atcagaagca gaagcacaaa gtcatcgttt
    10981 attattgaaa tcgggtttga taaaacaaag tacaagtggg atttatagtt atttaccgct
    11041 agcaacacgt gtgttaaata atattactgc aattgtgcga caagaaatgg aacgtatcga
    11101 ttctgttgaa attttaatgc cagcgttaca acaagctgaa ttatgggaag aatcaggacg
    11161 ttggggtgca tatggcccag aattaatgcg tttacaagat agacatggaa gacaatttgc
    11221 attaggtcca acacatgaag aattagttac atcaatagta agaaatgaat tgaaatcata
    11281 caaacaatta ccgatgacat tattccaaat tcaatctaaa ttccgtgatg aaaagagacc
    11341 acgttttggt ttacttcgtg gtcgtgaatt tattatgaaa gatgcgtatt cattccatgc
    11401 tgacgaggca tcattagatc aaacgtatca agatatgtat caagcgtata gccgtatttt
    11461 tgagagagtt ggcattaacg caagaccagt agttgcagat tcaggtgcta ttggcggtag
    11521 ccatacacat gaatttatgg cattaagtgc tatcggtgag gatacaatcg tttacagtaa
    11581 agaaagcgat tatgctgcta acatcgaaaa agcagaagtc gtttacgaac caaatcataa
    11641 gcatactact gtgcaaccta tagaaaaaat tgaaacacca aatgttaaga ctgcgcaaga
    11701 attggcagac tttttaggta gaccagtaga tgaaatcgtt aaaacgatga ttttcaaagt
    11761 tgatggcgaa tatattatgg ttttagtgcg tggacatcat gaaattaatg acattaaatt
    11821 aaaatcttat ttcggcacag ataatattga attagcaaca caagacgaaa ttgttaattt
    11881 agttggtgca aatcctggtt cactaggtcc tgtaattgat aaagaaatca aaatttatgc
    11941 agataatttt gtgcaagatt taaataattt agttgtcggt gctaacgaag atggttatca
    12001 cttaattaat gtaaatgtag gtagagactt caacgttgat gcatatggcg atttccgttt
    12061 tattttagaa ggcgaaaagt taagtgatgg ttcaggcgtt gcacattttg ctgaaggtat
    12121 tgaagttggt caagtattta aattgggtac taagtattca gaatcaatga atgctacatt
    12181 cttagataat caaggaaaag ctcaaccgtt aattatgggt tgttatggta ttggaatttc
    12241 tagaacgcta agtgcaattg ttgaacaaaa tcacgatgat aatggtattg tttggcctaa
    12301 atcagttact ccatttgatt tacatttaat ttctattaat cctaagaaag atgatcaacg
    12361 agaactagcg gatgcactat atgctgaatt taatactaaa tttgatgtgt tgtacgatga
    12421 tcgtcaggaa cgtgcgggtg tcaaatttaa tgatgccgat ttaattggtt taccactgcg
    12481 aattgttgtt ggtaaacgtg catcggaagg tattgtagaa gttaaagaac gtttaacagg
    12541 tgatagcgaa gaagtccaca ttgatgactt aatcactgtc attacaaata aatatgataa
    12601 cttaaaataa ttaagatcga atgaattata agagtaggaa aaagctgaaa gaaatcagat
    12661 gcttatgtcc tgctcttatt atttttgata taatgatt
//
LOCUS       DAHHOJ010000076        12681 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.076, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000076 DAHHOJ010000000
VERSION     DAHHOJ010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12681)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12681)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12681
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.076"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            196..1614
                     /locus_tag="NBH32_001837"
     CDS             196..1614
                     /locus_tag="NBH32_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCD1515430.1"
                     /translation="MENTVKYRKFILPIVVGLLIWALTPFKPDAVDPTAWYMFAIFVA
                     TIIACITQPMPIGAVSIIGFTIMVLVGIVDMKTAVAGFGNNSIWLIAMAFFISRGFVK
                     TGLGRRIALHFVKLFGKKTLGLAYSIVGVDLILAPATPSNTARAGGIMFPIIKSLSES
                     FGSKPKDGSARKMGAFLVFTEFQGNLITAAMFLTAMAGNPLAQNLASSTSNVHITWMN
                     WFLAALVPGLVSLIVVPFIIYKIYPPTVKETPNAKSWAENELATMGKIDLAEKFMIGI
                     FVIALTLWIVGSFIHIDATLTAFIALALLLLTGVLTWQDILNETGAWNTLVWFSVLVL
                     MADQLNKLGFIPWLSKSIATSLGGLSWPIVLVILILFYFYSHYLFASSTAHISAMYAA
                     LLGVAIAAGAPPLFSALMLGFFGNLLASTTHYSSGPAPILFSSGYVTQKRWWTMNLIL
                     GFVYFIIWIGLGSLWMKVIGIF"
     gene            complement(2027..2935)
                     /gene="rarD"
                     /locus_tag="NBH32_001838"
     CDS             complement(2027..2935)
                     /gene="rarD"
                     /locus_tag="NBH32_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCD1515431.1"
                     /translation="MNTEYKKGVFLALSAYILWGILPIYWQFVDAIGAFEILAFRIIF
                     SVIFMIFILAVGQKQRNAFQRDMNQLLAKPIQLLAIVVAGYVITLNWGTFIWAVTNGH
                     VLQTSLGYYINPLVSILLALIFLKERFNKFEWLAILFAFIGVLYMTLKIGEFPIVSII
                     LALSFGTYGLLKKVVHIDAISSITIECIVTAPAGLIYVIYLWQQHQMSFGLNMSSFWL
                     LFSGAITAIPLILFSAGAKRIPLSLIGFIQYVGPTIMFVLGIFVFKEPFSIDQLITFI
                     FIWTGIVLYSLSQYIKLKKHPVAKTL"
     gene            3156..3731
                     /locus_tag="NBH32_001839"
     CDS             3156..3731
                     /locus_tag="NBH32_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1515432.1"
                     /translation="MTLPKIGKPATRALNSQGIYTLEAVSQYTKSSLMEMHGVGPKAI
                     SILEQALFQHQLHFKTEVHSSLPFKLTGDVSCNHAPKRQQMIDFIVATAALDIELLRS
                     LVTTEFIWSVPGRFDIYGPQILIQELSNHYNQVASLNIHSSITHGCLGSMHGIEILKT
                     GKEIHFAHFFEFENHKKDAKLSKVTSYIVVD"
     gene            3897..4913
                     /locus_tag="NBH32_001840"
     CDS             3897..4913
                     /locus_tag="NBH32_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HCD1515433.1"
                     /translation="MTVFKNQRLSWLPYIAIVILLHVIGFSFLWIAGKDHHILFGMGI
                     LAYTLGLRHAFDADHIAAIDNTVRKLLQQRKDPSGVGFYFSIGHSSVVFLMAVFLGVS
                     VKWAKDELPHFQDIGGTIGTLVSGFFLVLIGVLNLIILISLINLFAKLRREHIEEAEV
                     DALLESRGLVSRFVGPYFKLITRSWHVLPLGFLFGLGFDTASEIALLALSSGASQQAI
                     SFIGILSLPILFASGMSLLDTLDGVVMKYAYNWAFFNPIRKIYYNITITAISVMAALV
                     IGMIELLQILADKLDLHGAFWAFIGSIEFDYLGYILVALFLITWLISSLIWKFGRIEH
                     KWSK"
     gene            5156..5956
                     /locus_tag="NBH32_001841"
     CDS             5156..5956
                     /locus_tag="NBH32_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arylamine N-acetyltransferase"
                     /protein_id="HCD1515434.1"
                     /translation="MNIAKLEDYLQIEPTRYNQPSIEALNYYATRFMLTVPFENIDVQ
                     NGKPISVDIDALFNKIVHDKRGGFCYELNTFFKAYLQQKGFNPELMSATIHTPGGGRS
                     LNGSHASLVVSINDVFYVTDVGFGDLPLHVIPITSSEHTQPITDISGTFRAIFNNEDK
                     DIFYVQKFENDHWHTKYEAEFKPKQIEDFNSNIEYNQTNPDSIFVQHLLITMPQSFGR
                     ATMSENHLTLTKAKDGISEKLAVTKDNYKHFLEKYFGLDITINRIEKQ"
     gene            complement(6090..6602)
                     /locus_tag="NBH32_001842"
     CDS             complement(6090..6602)
                     /locus_tag="NBH32_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HdeD family acid-resistance protein"
                     /protein_id="HCD1515435.1"
                     /translation="MAKANQGIKWSSLIMGVLLLMLAVVIFTFPIENFYAITWLIGLF
                     VLINGVIQIIYRRKAKALVGGNQNWILFMGIVDILFGLIVIFNVGASSAFFIYMFAFW
                     FIFSSIAGLFTFTGSGSLKLISVIFNLLGIVFGVILLFNPLMGIVFISTMIAIAFVFV
                     GVIYVVDALA"
     gene            6892..7650
                     /gene="vraD"
                     /locus_tag="NBH32_001843"
     CDS             6892..7650
                     /gene="vraD"
                     /locus_tag="NBH32_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter ATP-binding
                     subunit VraD"
                     /protein_id="HCD1515436.1"
                     /translation="MTILSVQHVSKTYGKKHTFQALKDINFDIQKGEFVAIMGPSGSG
                     KTTLLNVLSSIDQISSGSVIANGQELNKLNQKALAKFRKESLGFIFQDYSILPTLTVK
                     ENIMLPLSVQKMSKATMEENYKAITTALGIYDLGNKYPSELSGGQQQRTAAARAFVHK
                     PQIIFADEPTGALDSKSANDLLQRLEEMNKSFDTTIVMVTHDPVAASFAERVIMLKDG
                     QIHTQLYQEGRSKQAFYEDIVHLQSVLGGVSNDI"
     gene            7640..9520
                     /gene="vraE"
                     /locus_tag="NBH32_001844"
     CDS             7640..9520
                     /gene="vraE"
                     /locus_tag="NBH32_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter permease
                     subunit VraE"
                     /protein_id="HCD1515437.1"
                     /translation="MTFNHIVLKNLRQNLKHYAMYLFSLFFSIVLYFSFTTLQFTKGV
                     NNDDSMAIIKKGALVGSIFLFIIIVIFLMYANHLFVKRRTREFALFQLIGLTRQNILK
                     MLALEQMIVFLITGVVGVLCGIAGAQLLLSIVSKLMSLSINLSIHFEPMALVLTIFML
                     IIAYVLILFQSALFLKRRSILSMMKDSIKTDVTTAKVTTAEIISGVLGIAMIALGYYM
                     ATEMFGMFKALTMAMTSPFIILFLTVVGAYLFFRSSVSLIFKTLKKSKNGRVSITDVV
                     FTSSIMYRMKKNAMSLTIIAIISAVTVTVLCFAALSKSNTDQTLTSMAPNEFNVVATQ
                     DAKQFETKLSQQQITFSKNAYETITVDNVKDQVITLENGSDSGRTNSILSANNKLTGN
                     NAIITNTKSLPNVINIHLNKDLVVKGTKNETFRVTQEDKGKVYPLNLSFNSPVVEVSP
                     EKYQQLKTQNNVHTFYGYDIKQTSQKEKAQAIAKQFGDKVITYDEMKKEVDATNGILI
                     FVTSFLGLAFLVAAGCIIYIKQMDETEDELSNFRILKRIGFTHTDMLKGLLLKITFNF
                     GLPLLIAILHAVFAAIAFMKLMGNISFMPVIVVIVVYTLIYITFALIAFVHSNKLIKK
                     TI"
     gene            9612..9803
                     /gene="vraH"
                     /locus_tag="NBH32_001845"
     CDS             9612..9803
                     /gene="vraH"
                     /locus_tag="NBH32_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide resistance ABC transporter activity
                     modulator VraH"
                     /protein_id="HCD1515438.1"
                     /translation="MKFRELVKQSYEDLKNLTVTWYNVIALTILVIVLSSFTTPMIGI
                     PAGLLGGSYYLKRREEKGK"
     gene            10196..10396
                     /locus_tag="NBH32_001846"
     CDS             10196..10396
                     /locus_tag="NBH32_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1515439.1"
                     /translation="MNNGTVKWFNAEKGFGFIEQENGGDVFVHFSGIASDGYKTLEEG
                     QKVTFEITEGQRGDQAVNVQTV"
     gene            complement(10514..11083)
                     /locus_tag="NBH32_001847"
     CDS             complement(10514..11083)
                     /locus_tag="NBH32_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1515440.1"
                     /translation="MNLSKQIKKYRERDGYSQEYLAEKLYVSRQSISNWENDKSLPDI
                     HNLLMMCELFNVTLDDLVKGTIPFVPDIKVQRSLNLWTYVMLIFMTLAAILMGPLVVY
                     WNWTWGITVAIILGIGFYASMKIEDLKKVHKMDNYDRIVAFMNGKDPSEVQTTKARNT
                     MTNALSIISVVGTLSLIIFLSVYLANKFL"
     gene            complement(11343..11738)
                     /locus_tag="NBH32_001848"
     CDS             complement(11343..11738)
                     /locus_tag="NBH32_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD1515441.1"
                     /translation="MKLTLMKFFVGGFAVLLSYIVSVTLPWKEFGGIFATFPAVFLVS
                     MFITGMQYGDKVAVHVSRGAVFGMTGVLVCILVTWMMLHMTHMWLISIVVGFLSWFIS
                     AVCIFEAVEFIAQKRLEKHSWKAGKSNSK"
     gene            complement(11806..12159)
                     /locus_tag="NBH32_001849"
     CDS             complement(11806..12159)
                     /locus_tag="NBH32_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3147 family protein"
                     /protein_id="HCD1515442.1"
                     /translation="MFSIGSAILHFVIGGIAVALASIIADKVGGKLGGIIATMPAVFL
                     AAIIALALDHRGTQLVEMSMNLSTGAIVGILSCILTVFLTSLYIKHKGYRKGAIFTVV
                     CWFVISLAIFSIRHL"
BASE COUNT         4277 a         2219 c         1968 g         4217 t
ORIGIN      
        1 tattagttca ttttcaaaat cttttaaatt taataaacac catctatacc agttcttcac
       61 cgattatcga aaaataatta tattaatgat ttcgttaatt taattttata tttaattatt
      121 actgtacatc ttttgtagtt agctttattc ttaaattgaa atatgtataa aacatagaaa
      181 gaaggacgtt ttaacatgga aaacacggtt aaatatcgta agtttatact ccctatcgtt
      241 gtaggtctcc ttatttgggc acttacacct tttaaaccgg atgctgtgga tccaacagca
      301 tggtatatgt tcgcaatatt cgtcgcgaca atcattgctt gtattacaca accgatgcca
      361 attggggccg tctctataat tggatttaca atcatggtac tcgttggaat tgttgacatg
      421 aaaacggctg tcgctggttt tggtaataat agcatttggt taattgctat ggcatttttc
      481 atttcgagag gatttgtgaa aacaggtctt ggtagacgga tcgcacttca tttcgtcaaa
      541 ttatttggta aaaaaacatt aggattagca tattctatcg tcggtgtaga tttaattcta
      601 gcgcctgcta caccaagtaa taccgcgcgt gctggtggaa tcatgttccc aattatcaaa
      661 tcactttctg aatcatttgg ttcgaaaccg aaagacggat cagcacgcaa aatgggtgca
      721 tttcttgttt tcacagaatt ccaaggtaat ttaattactg cggctatgtt tttaactgca
      781 atggccggta acccccttgc acaaaattta gcatctagca catctaatgt tcacattaca
      841 tggatgaatt ggttcctagc tgctttagtt cctggacttg tttccttaat tgttgtacct
      901 tttattattt ataaaattta tccaccaact gttaaagaaa caccaaatgc taagagttgg
      961 gctgaaaatg aattagcgac tatgggtaaa atcgatttag ctgaaaaatt tatgattggt
     1021 atttttgtca ttgctctaac attatggatt gttggaagtt tcattcatat tgatgcaact
     1081 ttaacggcct ttattgcgct agcattgtta ttattgacag gcgtcttaac atggcaagac
     1141 attttaaacg aaacaggtgc ttggaacaca ttagtatggt tctcagtatt agtgttaatg
     1201 gccgaccaat taaacaagct tggatttatt ccttggttaa gtaaatccat tgctacaagt
     1261 cttggtggct taagctggcc tatagtactg gtcattttaa tattgttcta cttctattca
     1321 cattacttat ttgcaagttc tacagcacat atcagtgcga tgtatgcagc attactaggc
     1381 gttgccatcg cagccggtgc accaccatta ttcagtgcat taatgttagg tttcttcggt
     1441 aacctattag cttcaacaac acactatagt agtggtccag cgccgattct attctcttca
     1501 ggttacgtga ctcaaaaacg ttggtggaca atgaacttaa tattaggttt cgtctacttt
     1561 attatctgga ttggtttagg atcactttgg atgaaagtaa ttggtatatt ttaaaatatt
     1621 taaattagcg ctcgaatctc attgatttgg gcgcttttta atttgtattt aaaatcaatc
     1681 tttgctaaat caagactccc tttttaaaat acgtttatcc tttaaatcat tgcctacttc
     1741 actgaaaatt tgtataaaga tttaagtcat tacgtaacat cacataaaat acatttctat
     1801 actaatccgt ttcattgatc aacattacgt atgccctcat aaatcatgaa ataaaaaaca
     1861 ccttcgttta aattcatttt aagtgcgaat tcaacgaaag tgctttattt catatttaat
     1921 gtttcaaatt tatacggcta tcactgttac tgcacacata cctcagtctc accttatcat
     1981 gacacaaagg aaattgaaaa cacgtataat tatctctcct cgtattttat agggtttttg
     2041 cgaccggatg tttcttcaat ttaatgtatt gagaaagact atataacaca atacctgtcc
     2101 aaataaatat aaacgtaatt aattgatcta tactaaaagg ctctttgaaa acaaatatgc
     2161 cgagtacaaa cattattgtt ggtccaacgt attgaataaa tcctattaac gaaagtggaa
     2221 tacgttttgc cccggctgag aataggatta gtggtattgc cgtaatagca ccagaaaata
     2281 acaaccaaaa tgatgacatg ttcaatccaa atgacatctg atgttgctgc cataaataaa
     2341 taacgtatat gagtccagca ggtgcggtaa caatacattc aatcgtaata ctgctgatgg
     2401 catcaatatg tactactttt ttcaataatc catatgtacc aaaggataac gctaatataa
     2461 tagagacgat tgggaattct ccaattttca gtgtcatata taatacaccg atgaatgcga
     2521 ataaaatggc tagccattca aatttattga atctttcttt taaaaagata agtgcgagca
     2581 aaatgctaac aagtggattt atataataac ctaaacttgt ttgtaggacg tgtccgttcg
     2641 ttacagccca aataaatgta ccccaattta atgtaatgac atagcctgct acgacaattg
     2701 ctagtaattg aatgggcttg gctaacaatt gattcatatc tcgttgaaat gcattgcgtt
     2761 gtttttgtcc aaccgcgagt atgaaaatca tgaatattac tgaaaatata atacgaaagg
     2821 ctaaaatttc aaatgcgcct attgcatcaa cgaactgcca atatataggt agtattcccc
     2881 acagaatgta tgcactgagt gctaaaaata cacctttttt atattctgta ttcacttaca
     2941 tacctcctta ctctcccaat ttttaacatg ttacgctcac ttagttaagc taatataacg
     3001 attttactaa taatatttcg ataaagatat cattttgttt atatttccca catttattca
     3061 ccaaccacta aacaatatta attttataaa taattctgta caaatcaggg tatattgcca
     3121 gaaagactac catacaacat aaaggatgga tacaaatgac tttacctaaa attggaaagc
     3181 ctgcaacacg cgcgctaaat tcacaaggta tatacacatt agaagcagta tcacaatata
     3241 cgaagtcatc tctaatggag atgcatggcg ttggtcctaa agctatatca atattggaac
     3301 aagctttatt tcagcaccaa ctacatttta aaacggaagt tcattcatca ttacctttta
     3361 agttaacagg agatgtgtca tgcaatcatg cgcctaaacg tcaacaaatg atagatttta
     3421 tagttgctac agcagcatta gatattgaat tattacgttc actggttaca acagaattta
     3481 tttggtctgt ccctggccgc tttgacatat acggacctca aatattaatt caagaactat
     3541 ctaatcatta caatcaggtt gctagtctca atatccattc cagcatcaca catgggtgct
     3601 taggttccat gcatggtatt gaaatattaa aaactggtaa agagattcat tttgctcatt
     3661 tttttgaatt tgaaaatcat aaaaaggatg ccaagttaag caaagtgaca tcttacattg
     3721 ttgttgatta atgtatcaaa ttatatatat ctcaaaaaat tcttttgcgg ttacatgtat
     3781 atttttatat aaaaaataat tttgtataac atttttgtta tatgaacatt tattactaga
     3841 gtgcacattg aacatacagt atgattaaat atcatgttat gaaagaagga tatattttga
     3901 ctgtttttaa aaatcagcgt ttaagctggt taccatacat agctattgtc attttgttac
     3961 acgttattgg gtttagtttt ttatggattg ctggaaaaga ccatcatatc ttatttggta
     4021 tggggattct tgcatataca ttaggtttgc gtcatgcatt tgatgcagat catatcgccg
     4081 cgatagataa tacggttcgc aaattattac agcaacgcaa agatccatct ggtgtaggtt
     4141 tctatttttc aattggacat tcatctgtcg tatttttaat ggctgttttt ttaggggtat
     4201 ctgtaaaatg ggctaaagat gaattaccgc atttccaaga tattggtgga acgattggta
     4261 cactagtttc aggtttcttt ttagtgctta tcggtgtgtt gaatctaatt attttaatct
     4321 ctttaattaa cttgttcgct aaattacgtc gtgaacacat tgaagaagct gaagtcgatg
     4381 cattacttga atctagagga ttggtttctc gatttgtagg accttatttc aaattaatca
     4441 cgcgtagttg gcacgtattg ccacttggct ttttatttgg acttggtttt gatacagcta
     4501 gtgaaattgc gttactcgct ctttcttcag gcgcatcaca acaagccatt tcatttatcg
     4561 gaatcttatc tttaccaatt ttattcgcat caggtatgag tttattggat acattagatg
     4621 gtgttgtaat gaagtatgcc tataattggg cattcttcaa tcctattcgc aaaatctatt
     4681 acaatataac gattactgcg atatcagtca tggcagcatt agtgattggg atgattgaat
     4741 tgctacaaat tcttgctgat aagctagatt tacatggtgc gttttgggca ttcattggtt
     4801 cgatcgaatt tgattattta ggttatattt tagttgcatt atttttaatt acttggctta
     4861 tttcaagttt aatttggaag tttggtcgca tagaacacaa atggtctaaa taaagtaact
     4921 tgtcttgcaa ctacctttgt attgttgcat ttttagacac cctgcatact gactcattta
     4981 ggatatgctc tctgttgtca tctttaaatc gtttctttta gagatgactt cattttgaga
     5041 gcgtattttt ctatttaaaa tgtatttatc caaaattcaa tgctttcgaa tttaaaattt
     5101 cagcatttct gtttaaaatt taattataat ctttaataca ggaggcggta tactaatgaa
     5161 tattgcaaag ttagaagatt atctccaaat tgaaccaact cgttataatc aaccgagtat
     5221 tgaagcatta aattattatg cgactcgctt tatgttaacg gtgcctttcg aaaatattga
     5281 tgttcaaaat ggtaaaccaa tatctgtaga tatcgatgca cttttcaata aaattgttca
     5341 tgataaacgc ggtggttttt gttatgagtt aaatacattt ttcaaagcat atttacaaca
     5401 aaaaggattt aatcctgaat taatgtcagc tactattcac acacccggtg gcggtcgtag
     5461 tctgaacggt tcacatgctt cacttgttgt ttctataaat gatgtattct atgtaactga
     5521 tgttggcttt ggagacttac ctctacatgt cattcctatt acttcatctg agcatacaca
     5581 accaattact gatatcagtg gtacattccg cgctattttt aacaacgaag acaaggatat
     5641 tttctatgtt caaaaattcg aaaatgatca ttggcataca aaatatgaag ctgaattcaa
     5701 acctaaacaa atagaagatt ttaatagcaa catcgaatat aaccaaacca acccagattc
     5761 aatatttgta cagcatttat taattacaat gccacaatca tttggacgcg ctacaatgtc
     5821 tgaaaatcat ttaactttga caaaagcaaa ggatggcatc tcagaaaagt tagctgtcac
     5881 taaagataac tataaacatt ttttagaaaa atattttgga ctagatataa ctattaatcg
     5941 tattgaaaaa caataatagt aaaatataaa aatatgggcg tatcttactt attgtaggta
     6001 gcgccccttt tttatgcttc ataaacacaa caaaaaggat atgacacaaa cttcatgtca
     6061 cacccttctt ttgaaccgct tcattttact taagcaagtg catctacaac ataaatgaca
     6121 cctacgaata caaatgcaat agcaatcatc gtcgaaataa agacgatacc cattaatgga
     6181 ttaaataata aaatgacacc gaaaacaata cctaataaat taaaaatcac tgaaattaat
     6241 tttaagctac cactacccgt aaacgtaaat aatcctgcaa tagaactaaa aatgaaccag
     6301 aatgcaaaca tgtagataaa aaatgccgag ctagcgccaa cattaaaaat gacaataaga
     6361 ccaaagagaa tatctacaat ccccataaac agaatccaat tttggttacc acctactaaa
     6421 gcttttgctt ttctacggta aatgatttga atcacaccgt taattaatac aaacagtcca
     6481 atcaaccagg taatagcata aaaattttca attgggaatg taaaaataac aactgccaac
     6541 attaataata atacacccat tattaaacta gaccatttaa taccttggtt tgcttttgcc
     6601 ataaaaatca attcctttca aataaagccc tctttatatt ttcattagtg ccactattat
     6661 tcattgttta aattccctca tcttaactta tagaaacatt tcctcactgc taatactgtg
     6721 atgctcagat tagttgaatt gctttccaat aatccaaatt cacccgagaa aatcacgata
     6781 tattaatatc tttcaactat gtaagattca aacattgaat tgtaagttta gataaaggct
     6841 actactccaa acattattta aaatgaaatt aaatacaaaa ggagtgatac tatgacgata
     6901 ttatcagtgc aacatgtttc aaaaacatac ggtaaaaagc acacatttca agcacttaaa
     6961 gatattaact ttgacataca aaaaggcgaa ttcgttgcga ttatggggcc ttctggatca
     7021 ggtaagacaa ccttattaaa tgtactaagt tcaattgacc aaatttctag cggtagcgtg
     7081 attgctaacg gacaagagct taataaactt aatcaaaaag cacttgccaa attccgtaaa
     7141 gaatcattag gtttcatctt ccaagattac agtattctgc cgacattaac tgttaaagaa
     7201 aacattatgt tacctttatc tgttcaaaaa atgtcgaaag caacaatgga agaaaattat
     7261 aaagcgatta ctacagcatt aggtatttat gacctgggaa ataaataccc tagcgaatta
     7321 tctggtggtc aacaacaaag aactgcagca gcgagagcat ttgttcataa accacaaatc
     7381 atatttgcag atgagccaac aggcgcactc gactcgaaaa gtgcaaatga cttattacaa
     7441 cgtttggaag aaatgaataa atcgtttgat acaactattg tcatggttac acatgatccg
     7501 gttgcagcaa gttttgcaga acgtgtcatc atgctaaaag atggccaaat tcatacacaa
     7561 ctttatcagg aaggacgttc taaacaggcc ttttatgaag acattgtaca tcttcaatca
     7621 gtattaggtg gtgtctcaaa tgacatttaa ccatatcgtt ttaaaaaact tacgtcaaaa
     7681 cttaaaacat tatgcaatgt atttattttc attatttttt agcatcgtct tatatttcag
     7741 ttttacaacc ttacagttta ctaaaggtgt aaataatgat gactcgatgg ccatcattaa
     7801 gaaaggtgct ttagtcggat caatattttt atttatcatt attgtcatct ttttaatgta
     7861 tgccaatcat ttattcgtga aacgccgtac acgtgaattt gcgctatttc agttgattgg
     7921 tttaacacga caaaacattt tgaaaatgtt agcacttgaa caaatgattg tatttttaat
     7981 cacaggtgtt gtcggtgtat tatgtggcat tgcaggtgca caattattgc tttccattgt
     8041 atctaaattg atgtcgttat cgattaactt atcgatacat ttcgaaccta tggcccttgt
     8101 tttaactatt ttcatgctaa ttattgcgta tgtactgatt ttatttcaaa gtgctttatt
     8161 tctaaaaaga cgtagtatct tatcaatgat gaaagactca attaaaactg atgttacaac
     8221 tgctaaagta acgactgcag agattatttc aggcgtatta ggtattgcta tgattgcact
     8281 aggatattat atggcgacag aaatgtttgg tatgttcaaa gcactaacta tggctatgac
     8341 atcaccgttt atcattttat ttttaacggt tgtaggcgcc tatttattct tcagaagttc
     8401 cgtgtcactt atttttaaaa cattgaaaaa atcaaaaaat ggacgcgtat ctattaccga
     8461 tgttgtattc acatcgtcta ttatgtacag aatgaagaaa aatgccatgt ctttaactat
     8521 cattgcaatt atttctgcag ttacagtaac tgtattgtgt ttcgcggcat tatctaaatc
     8581 aaatacagat caaaccctta catctatggc accaaatgaa tttaacgtgg tcgctactca
     8641 agatgctaaa caatttgaaa ctaaactaag ccaacaacaa attacatttt ctaaaaatgc
     8701 ttacgaaact attactgtag ataatgttaa agatcaggtt attactttgg aaaatggtag
     8761 tgattcaggt cgcacgaatt caatattaag tgcaaataac aagttaacag gcaacaatgc
     8821 catcattaca aatacaaaat cgctgcctaa cgtgattaat attcatttaa acaaagattt
     8881 agtagtaaaa ggtacaaaga acgagacttt ccgtgttaca caagaagaca aaggtaaggt
     8941 ttatccttta aatctaagct tcaactcacc tgtcgtcgaa gtaagtcctg aaaagtatca
     9001 gcagttgaaa acacaaaata acgttcatac tttttatgga tatgatatta aacaaacatc
     9061 acaaaaggaa aaagctcaag caattgcaaa acagtttgga gacaaagtca tcacgtatga
     9121 tgaaatgaaa aaagaggtcg atgctactaa cggtattcta atatttgtta catcattttt
     9181 aggattagca tttttagtag cagctggatg tattatttat attaaacaaa tggatgaaac
     9241 tgaggatgaa ctaagtaact tcagaatatt aaaacgcata ggctttacgc atacagatat
     9301 gcttaaggga ttattattaa aaattacttt caacttcggt ttaccattat tgattgctat
     9361 attacatgca gtattcgccg caatagcatt tatgaaattg atgggtaata tttccttcat
     9421 gccggttatt gtagtaattg ttgtatacac actcatttac attacttttg ctttaattgc
     9481 ttttgtacat tcaaacaaat tgattaagaa aaccatttaa cacttacgat taaaagatgc
     9541 ttcattgaga ttttgatttt aagcaatgca gtataactat taactgattt ataaaaagga
     9601 gagattgaat catgaaattt agagaattag taaaacaatc atacgaggat ttgaaaaatt
     9661 tgactgtaac ttggtataac gtcattgctt taacaatact tgttattgta ttaagctcat
     9721 tcacaacacc aatgataggc attccagctg gtttattagg tggctcctat tatttaaaac
     9781 gtcgtgaaga aaaaggcaag taagcacatt ccatcggtat agaacattgc tatgaccttt
     9841 caagtcagat tttaatatca acaagtagcg tttatcgctg cttgtttttt atggattgct
     9901 tataaaatca tcatgtttaa acgatatgtc actttgaaaa ctagcaaaca ccataaaaga
     9961 ctacaatttt tttattttta taaacccctt taaatcaacg tttctaaggt ttttaaaaat
    10021 cttttttaat ttattttcaa aaaacactgt acattatgcc aatatgagcg tatagttggt
    10081 cttacgtaat aaaagctcgt gaattaaatt gtagtgtatt tgtttagaat atcctctttt
    10141 ttagttatga atttgttaca aatattaagt gcaaaagcac atggaggttt tctatatgaa
    10201 taacggtaca gtaaaatggt ttaacgcaga aaaaggtttt ggtttcatcg aacaagaaaa
    10261 tggcggagac gtattcgtac atttctcagg tatcgctagc gatggctaca aaactttaga
    10321 agaaggtcaa aaagttactt tcgaaatcac tgaaggtcaa cgtggagacc aagcagttaa
    10381 cgtacaaact gtttaatctt acaaaataaa acgactcatt ataaatgaat cgctttgatt
    10441 accaacaagg tttagaacct tgttggtatt tttttatgct atttcggata atatgagacg
    10501 atacgatgat gatttataaa aatttatttg ccaaatacac actaaggaaa attatgaggc
    10561 tgagtgtacc aactactgat ataatagaaa gcgcatttgt catcgtattt ctagctttag
    10621 tcgtttgtac ttcactagga tcttttccat tcataaaagc aacaattcga tcgtagttgt
    10681 ccattttatg cacttttttt aaatcttcta ttttcataga tgcataaaag cctattccca
    10741 aaatgattgc caccgttata ccccaagtcc aattccaata aacaactaaa ggtcccatta
    10801 aaattgcagc taatgtcatg aaaataagca tcacatatgt ccataagtta agacttcgtt
    10861 gcactttaat atcaggtaca aatggaatag tcccttttac taaatcatct aaagttacat
    10921 tgaacaattc gcacatcatt aataagttat gtatgtctgg taagcttttg tcattttccc
    10981 aattagaaat actctgccta gatacatata acttttcagc aagatattct tgtgaataac
    11041 catctcgttc cctatatttt ttaatttgtt tactcagatt caattacata cgcctccatt
    11101 caattagtac aatcattaca atcagactat cagataaagt taaatcaagt cacctgtatg
    11161 attgactaaa tgttttgaca tacgacaaca tcggggttcg aagactccct taagacagac
    11221 ctgtatgatt acgcgcttgt aattttctca atgccaactt gaatttcact tattaatgat
    11281 gcaacttttc catgctattc aatgcttgca aagttagtcc tagttaagag attacgttca
    11341 cactatttac tattcgattt tccagctttc caactatgct tttctaatct tttttgtgct
    11401 ataaattcta ccgcttcaaa aatacatact gcactgatga accagcttag gaaaccaaca
    11461 acaatgctaa tcaaccacat gtgcgtcata tgtaacatca tccatgtaac taaaatacaa
    11521 actaataccc ctgtcatacc aaacactgcg ccacgactta catgcacagc gactttatca
    11581 ccatattgca tacctgtaat aaacatagac actaaaaata ctgccggaaa cgttgcaaat
    11641 ataccgccaa attctttcca aggtagtgtt acagatacaa tataacttaa taatactgca
    11701 aatcccccca caaaaaattt cattaatgtt aatttcatga tgttctgcct ccaataattt
    11761 attacttaat aactgaatac aactaattat cacgcatttt ccaaactata aatgtctaat
    11821 actgaatatt gcgagggaaa tgacaaacca acaaacaact gtgaatattg cgcctttccg
    11881 ataaccttta tgcttaatgt agagagatgt caaaaataca gttaatatac aagacagaat
    11941 accgacaatt gctccagtac taagattcat cgacatctcc actaattgcg taccacgatg
    12001 atctaaagct aatgcgataa tagccgcaag aaagactgcc ggcatagtag ctataatacc
    12061 tcctaactta ccacctacct tatcagcaat aattgaagct aatgcaacag cgataccacc
    12121 aatgacaaaa tgaagaattg cacttccaat tgaaaacatc ttcaacgtac ctttctataa
    12181 ttaattcaat acaaaacgta tcattcacac aaagtgtaaa acttaaattt tctactgtaa
    12241 aggcaactca agcgatgttc ataaagcttt catgatactt tcataaacta gcaataaagg
    12301 gttcaaatga aagtatatcc agctcaaatt agggagtggg acagaaatga tattttcgca
    12361 aaatttattt cgtcgtccca ccccagcaag gttgactaga attgaaaaaa gcttgttaca
    12421 agcgcatttt cgttcagtca actactgcca atataacttc gtagagcata gaacattgat
    12481 ttatgtacca gcctgatcaa catataaata taaattttta tgtttcacgt aaaacaaatc
    12541 aaaaccaccc gtgtgaacgg gtggtttgtt cagcggctat aagccttcct tactggccag
    12601 ccctaaaagg gcactgaaaa gtcagccaac tgcactacta ttccagcaac cctaaagggt
    12661 tactcttttt tctttctttt t
//
LOCUS       DAHHOJ010000077        12522 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.077, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000077 DAHHOJ010000000
VERSION     DAHHOJ010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12522)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12522)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12522
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.077"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(61..1188)
                     /locus_tag="NBH32_001850"
     CDS             complement(61..1188)
                     /locus_tag="NBH32_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HCD1515443.1"
                     /translation="MKSKYEPLFDKVELPNGVELRNRFVLAPLTHISSNDDGTISDVE
                     LPYIEKRSQDVGITINAASNVSDVGKAFPGQPSIAHDSDIEGLKRLATAMKKNGAKAL
                     VQIHHGGAQALPELTPGGDVVAPSPISLKSFGQKQEHSAREMTNEEIEQAIKDFGEAT
                     RRAIEAGFDGVEIHGANHYLIHQFVSPYYNSRNDVWANQYKFPVAVIEEVLKAKEAYG
                     NKDFIVGYRLSPEEAESPGITMEITEELVNKISHMPIDYIHVSMMDTHATTREGKYAG
                     QERLPLIHKWINGRMPLIGIGSIFTADEALDAVENVGVDLVAIGRELLLDYQFIEKIK
                     DGREDEIINYFDPEREDNHHLTPNLWHQFNEGFYPLPRKDK"
     gene            complement(1550..1927)
                     /locus_tag="NBH32_001851"
     CDS             complement(1550..1927)
                     /locus_tag="NBH32_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001067296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HCD1515444.1"
                     /translation="MNNYKIGQHIKVRVTGIQPYGAFVETPNHTEGLIHISEIMDDYV
                     HNLKKFLSEGQIVKAKILSIDDEGKLNLSLKDNDYFKNYERKKEKQSVLDEIRETEKY
                     GFQTLKERLPIWIKQSKQAIRND"
     gene            complement(2343..2936)
                     /locus_tag="NBH32_001852"
     CDS             complement(2343..2936)
                     /locus_tag="NBH32_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCD1515445.1"
                     /translation="MANYPQLNKEVQQGEIKVVMHTNKGDMTFKLFPNIAPKTVENFV
                     THAKNGYYDGITFHRVINDFMIQGGDPTATGMGGESIYGGAFEDEFSLNAFNLYGALS
                     MANSGPNTNGSQFFIVQMKEVPQNMLSQLADGGWPQPIVDAYGEKGGTPWLDQKHTVF
                     GQIIDGETTLEDIANTKVGPQDKPLHDVVIESIDVEE"
     gene            3000..3383
                     /locus_tag="NBH32_001853"
     CDS             3000..3383
                     /locus_tag="NBH32_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase-associated lipoprotein B"
                     /protein_id="HCD1515446.1"
                     /translation="MKLYRFSHKTGSYGVTIVEENGDDVLVKVEQVIKHPKQGDLHNP
                     TETEGVFFHERKALSHFEKRYTKRSQLRDFNVDVLRYEDSLQQAITDLETKLKAQQTK
                     HAEMSLASLARLKEDYSLQYKENFK"
     gene            3514..5919
                     /gene="mnhA1"
                     /locus_tag="NBH32_001854"
     CDS             3514..5919
                     /gene="mnhA1"
                     /locus_tag="NBH32_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit A"
                     /protein_id="HCD1515447.1"
                     /translation="MSLLHIAVILPLIFALIIPILYRFFKRIHLGWFVLPVPIVIFIY
                     MLTLIKTTMSGNTVMKTLNWMPHFGMNFDLYLDGLGLLFSLLISGIGSLVVLYSIGYL
                     SKSEQLGNFYCYLLLFMGAMLGVVLSDNVIILYLFWELTSFSSFLLISFWRERQASIY
                     GAQKSLIITVFGGLSLLGGIILLAIPTQSFSIQYMIQHASEIQNSPFFIFAMILIMIG
                     AFTKSAQFPFYIWLPDAMEAPTPVSAYLHSATMVKAGLYLIARMTPIFAASQGWIWTV
                     TLVGLITLFWASLNATKQQDLKGILAFSTVSQLGMIMAMLGIGAISYHYQGDDSKIYA
                     AAFTAAIFHLINHATFKGALFMITGAVDHSTGTRDVKKLGGLLTIMPISFTITVITAL
                     SMAGVPPFNGFLSKESFLETTFTASQANLFSVDTLGYLFPIIGIVGSVFTFVYSIKFI
                     MHIFFGQYKPEQLPKKAHEVSILMLLSPAILATLVIVFGLFPGILTNSIIEPATSSIN
                     HTVIDDVEFHMFHGLTPAFLSTLVIYILGILLIVTFSYWVKLLQRQPGKLTFNYWYNR
                     SANVIPNYSEKMTNSYVTDYSRNNLVIIFGTLILLTFVTIFSVPFNINFKDVSPIRIF
                     EVCIVILLLSAAFLILFAKSRLFSIIMLSAVGYAVSVLFIFFKAPDLALTQFVVESIS
                     TALFLLCFYHLPNLNRYNEKRSFQLTNALIAGGVGLSVIIIGLIAYGNRHFESISKFY
                     QEHVYDLAHGKNMVNVILVDFRGMDTLFESSVLGIAGLAVYTMIKLRKKRQTQGNEVK
                     NHE"
     gene            5912..6340
                     /gene="mnhB1"
                     /locus_tag="NBH32_001855"
     CDS             5912..6340
                     /gene="mnhB1"
                     /locus_tag="NBH32_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit B"
                     /protein_id="HCD1515448.1"
                     /translation="MNRQQNDLILQFAAVIIFFMVMVFGFSLFLAGHYTPGGGFVGGL
                     LFASSLVIITIAFDIETMRKIFPLDFKILIGIGLVFCIATPIASWFLGKNFFTHVTFD
                     IPLFILEPVHMTTAVFFDFGVLCAVVGTVMTIIISIGENE"
     gene            6340..6681
                     /gene="mnhC1"
                     /locus_tag="NBH32_001856"
     CDS             6340..6681
                     /gene="mnhC1"
                     /locus_tag="NBH32_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit C"
                     /protein_id="HCD1515449.1"
                     /translation="MEIIMIFVSGILTAISVYLVLSKSLIRIVMGTTLLTHAANLFLI
                     TMGGLKHGTVPIYEANVKSYVDPIPQALILTAIVIAFATTAFFLVLAFRTYKELGTDN
                     VESMKGVPEDD"
     gene            6674..8170
                     /gene="mnhD1"
                     /locus_tag="NBH32_001857"
     CDS             6674..8170
                     /gene="mnhD1"
                     /locus_tag="NBH32_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit D"
                     /protein_id="HCD1515450.1"
                     /translation="MIESNMLVLTLVIPVITAILLVFIGKRPIIKRYVALGGTLLTLV
                     AAIINLANVVKHGPIRVELGSWKAPYSIVFVLDIFSALLIITSIIITAIVILYSYQTI
                     GIERERYYYYFSVLFMLIGIIGAFTTGDIFNLFVFFEVFLMSSYFLLVIGSTKIQLQE
                     TIKYVLVNVVSSSFFVMGVAILYSVVGTLNLADISNKLANLSAHDSGLVNIVFILFIF
                     VFATKAGVFPMFVWLPSAYYAPPIPIIAFFGALLTKVGVYAIARTLSLFFSDNVSFSH
                     YVILFLALLTIIFGCVGAVAYANIKKIILYNVMIAVGVILVGVAMMTESGMIGAIYYT
                     LHDMLVKLALFLLIGIMIKITGTADLRQFGGLIKRYPVLGWSFFIAALSLAGIPPLSG
                     FYGKFFIVQSTFERGFYLSGVIVLLSSLVVLYSVIRIFLQGFFGQPKGYDLNNKVDVK
                     YLTTIAIVAVVITVLYGLSADYLYPMVKAGAETFYNPSTYVKAVLGGK"
     gene            8172..8651
                     /gene="mnhE1"
                     /locus_tag="NBH32_001858"
     CDS             8172..8651
                     /gene="mnhE1"
                     /locus_tag="NBH32_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit E"
                     /protein_id="HCD1515451.1"
                     /translation="MAVQLVLNFIIAVFWLFVTNSYTTNNFVLGFIFGLVLVYLLHRV
                     LPGRFYVITLYRIIKLVIIFLIELIKANFDVLKIIIKPSIKNEPGFFVYHTDLKKDWQ
                     IVLLSNLITLTPGTVVLGVSDDRTKIYIHAIDFSTKEQEVESIKTSLEKIVREVGEI"
     gene            8651..8944
                     /gene="mnhF1"
                     /locus_tag="NBH32_001859"
     CDS             8651..8944
                     /gene="mnhF1"
                     /locus_tag="NBH32_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit F"
                     /protein_id="HCD1515452.1"
                     /translation="MNHNVIIVIALIIVVISMLAMLIRVVLGPSLADRVVALDAIGLQ
                     LMAVIALFSILLNIKYMIVVIMMIGILAFLGTAVFSKFMDKGKVIEHDQNHTD"
     gene            8922..9278
                     /gene="mnhG1"
                     /locus_tag="NBH32_001860"
     CDS             8922..9278
                     /gene="mnhG1"
                     /locus_tag="NBH32_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter Mnh1 subunit G"
                     /protein_id="HCD1515453.1"
                     /translation="MIKIILISLALIFVIIGALISALAAIGLLRLEDVYSRAHAAGKA
                     STLGAMSLLFGTFLYFIATQGFVNMQLIVAIIFVLITGPLSSHMIMKAAYNIKTPYTK
                     KTKVDEISEDLKDTKL"
     gene            9527..10681
                     /locus_tag="NBH32_001861"
     CDS             9527..10681
                     /locus_tag="NBH32_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD1515454.1"
                     /translation="MHWTIIGGGIQGTAIAQKLLSSGLTTDRLTIIDPHETFCQRFNS
                     FTNRIEMPYLRSPIVHHVHPQPFHLKQFAKQHQYTNAFYGPYQRPELTMFMDHIAHAS
                     KQYQLEDCLVQGLVQTLDKQEDKWHIKLEDGQIITTDCVVIAIGSTNIPFMPDILKDK
                     QNVNHIFEKEHDQVVYDKTDHIVGSGITAAHLALKLLNHDNDKKIHLWLNKDIEIHDF
                     DADPGWLGPKNMSTFLSTKSMPERNAIVQRERHKGSMPHELYLRLKKHIKNGRINVHK
                     TPITQISDGLINTENDSVPYQQIMVATGFEQDFMSQPLIKQLIQNYDAPINECNYPVI
                     SEKLEWIPNLFVAGCFADLELGPFGRNVMGGRKAAERIEQAFLKLQQYSA"
     gene            complement(10737..11111)
                     /locus_tag="NBH32_001862"
     CDS             complement(10737..11111)
                     /locus_tag="NBH32_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HCD1515455.1"
                     /translation="MTHLLETFEMSIDHQEDGLVVISMPVTDKVKQPFGYLHGGASIA
                     LGETACSLGSANLIDTTKFIPLGLEMNANHIHSAKDGRVTATAEIIHQGKSTHVWDIK
                     IKNDKEQLITVMRGTVAIKPLK"
     gene            complement(11130..12446)
                     /locus_tag="NBH32_001863"
     CDS             complement(11130..12446)
                     /locus_tag="NBH32_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter family protein"
                     /protein_id="HCD1515456.1"
                     /translation="MINAVVIAVILMIVLCLCRLNVVISLFISALVGGVVSGMSIEKV
                     INVFGKNIVDGAEVALSYALLGGFAALISYSGITDYLVGKIINAIHAENSRWSRVKVK
                     VTIIIALLAMSIMSQNLIPVHIAFIPIVIPPLLSLFNDLKIDRRLIGLIIGFGLCFPY
                     VLLPYGFGQIFQQIIQSGFAKANHPIEFNMIWKAMLIPSMGYIVGLLIGLYVYRKPRE
                     YETRKISDSDNVTELKPYILIVTIVAILATFLVQTFTDSMIFGALAGVLVFFISRAYN
                     WYELDAKFVEGIKIMAYIGVVILTANGFAGVMNATGDIDELVKTLTSITGDNKLFSII
                     VMYVIGLIVTLGIGSSFATIPIIASLFIPFGASIGLDTMALIALIGTASALGDSGSPA
                     SDSTLGPTAGLNVDGQHDHIRDTCVPNFLFYNIPLMIFGTIAAMVL"
BASE COUNT         3980 a         2163 c         1966 g         4413 t
ORIGIN      
        1 aatattgaaa accactaaca aattgtatac acaatatcat tagtggtaac agttaaacac
       61 ttatttatct ttacgtggta atggatagaa cccttcatta aattgatgcc ataaattagg
      121 agttaagtga tgattatctt ctctctctgg atcaaagtaa ttaataattt catcttcgcg
      181 tccatcttta attttctcaa taaattgata atccagtagt agctctctac caatagctac
      241 taagtcaaca ccaacatttt caactgcatc taaagcttcg tcagctgtga aaattgaacc
      301 aataccgata agtggcatac gaccatttat ccatttgtga attaaaggca gtctttcttg
      361 tccagcgtat ttaccttcac gtgtcgttgc atgcgtatcc atcattgaaa catgaatata
      421 gtcgattggc atatggctaa ttttattaac gagttcctct gtaatttcca ttgtgattcc
      481 tggagactcc gcttcttccg gagacaatct gtatccaact ataaagtctt tattgccata
      541 cgcttctttc gctttaagca cttcttcaat cacagcgacc gggaatttat attgatttgc
      601 ccatacatca tttctactat tatagtatgg tgatacaaat tgatgaatta agtaatgatt
      661 cgcaccatgt atttcaacac catcaaaccc tgcttcaatt gcacgtcgcg ttgcttcacc
      721 aaaatccttg attgcttgtt caatctcttc attcgtcatt tctctagcac tatgttcttg
      781 tttctgaccg aaacttttta aagaaattgg acttggtgct acgacgtctc caccaggtgt
      841 taattcaggc aatgcttgtg caccgccatg atgtatttgt acgagtgctt tggcaccgtt
      901 tttcttcatt gctgtagcta atcgttttag tccttcaata tcactgtcat gcgcgattga
      961 tggctgtcct ggaaatgctt ttccgacatc actcacatta ctcgcagcat taattgtaat
     1021 accaacatct tgtgaacgct tttcaatata aggaagttct acatctgaaa tagtaccatc
     1081 atcatttgaa gaaatatgtg ttaaaggggc taacacaaat cgatttctca actctactcc
     1141 atttggtaat tctactttat caaacaatgg ttcgtattta cttttcatta cttaattcct
     1201 ccttaaaatt attgagttga ctataacatg taattttttc attctccaat tatctgttta
     1261 tatagttgca attcgtttaa tactttaagt tgtagttctc atttctaaaa taaattaaaa
     1321 aaacaaatcc acatacaata ttgcaaatca acgattctat ctatcaaaga taatggatat
     1381 gtcaccttaa accatttgta cgtgtaaata gaatcaatca tattacgtta tcgtagacaa
     1441 aaagagatta gtatctaata aaatttaata gatactaatc tcttttatag tctaagaaac
     1501 tttcatttca gaccctttgt atgattttcg gtacgattta tctgttcctt tagtcgtttc
     1561 gaattgcttg ctttgactgt tttatccaga ttggtaagcg ttctttaagt gtttgaaacc
     1621 catatttttc tgtttctctg atttcatcta atactgattg tttttccttc ttacgctcat
     1681 aatttttgaa gtaatcatta tcctttaatg atagattaag ctttccttca tcatctatag
     1741 acaaaatttt agctttaaca atttggcctt ctgatagaaa tttcttcaaa ttatgaacgt
     1801 agtcatccat aatttctgat atatgaatca gtccttcagt atgattaggg gtctcaacaa
     1861 acgcaccgta tggttgaata ccagttacac gcaccttgat atgttggcca attttgtagt
     1921 tattcaactt aataacccct tatttcgttt acttaacact attatcatag cacaaatcga
     1981 aatggtggac taattaaaac atcaaatcat acgaatatta ttttataaat gaataagaat
     2041 actacaaact tggatttaca aataaaatca gcaacaaatt cctatctaaa tcaatatgat
     2101 atccagctac tttgttttca atttattaac tatttgaata gcaattctct gtaaaaacat
     2161 aaaaagcaac ctcaactatt atctttagaa tacacatcta tttaaaaact gtgctttctt
     2221 gataaatata ttgagattgc tcatcatgtt agtttatttg ttttcgttgg aatatatact
     2281 aatccattca taaattagct ttatccgagt ttaaaatgtt ggtagttaat tatgtttaga
     2341 ttttattctt caacatcaat agattcaatt acaacatcat gaagtggttt atcttgtggt
     2401 cccacttttg tatttgcaat atcttctaat gtagtttcac catcaatgat ttgaccgaat
     2461 acagtatgtt tttgatctaa ccatggtgta ccacccttct cgccatatgc atcaacgatt
     2521 ggttgtggcc aaccaccatc tgcaagttga cttaacatat tttgaggtac ttctttcatt
     2581 tgaacaatga aaaattgtga accatttgta ttaggtcctg agttagccat tgataatgcg
     2641 ccatataagt taaatgcatt taatgaaaat tcatcttcaa aagcaccgcc ataaatactt
     2701 tcgccaccca taccagtagc tgttggatcg ccaccttgaa tcatgaagtc attaatgaca
     2761 cggtggaatg ttattccatc ataataacca ttttttgcat gtgtcacaaa attttcaact
     2821 gtttttggtg caatatttgg aaataatttg aatgtcatgt cacctttatt tgtgtgcata
     2881 accactttaa tttcaccttg ttgtacttct ttgtttaact gtggatagtt agccatttat
     2941 attctccatt catgttatga tacatatata ttaacatact tttaagggag gaaaatagca
     3001 tgaagttata tcgattttca cacaaaacag gtagctatgg tgtcacgatt gttgaagaaa
     3061 atggagacga tgtccttgtt aaagttgaac aagttatcaa acacccgaaa caaggtgatt
     3121 tacataaccc tactgaaact gaaggtgtat ttttccatga acgcaaagca ttaagccatt
     3181 ttgaaaaacg ctatacgaaa cgttcacaat tacgcgactt caatgttgat gtattgcgct
     3241 atgaagattc acttcaacaa gcaattacag atttagaaac taaacttaaa gctcagcaaa
     3301 caaaacatgc tgaaatgtct ttagctagtt tagcacgctt aaaagaagat tattcacttc
     3361 aatacaaaga aaattttaaa taaaaaatga cgtgtttctt cttatttaaa tacttctgaa
     3421 aataaagcac aacaaggagt gtgtacgttt ttcagaacta tatcattcat gtataatgaa
     3481 taacataaat attttttaaa ggaggacaac atagtgagtt tgttacatat tgcggtgata
     3541 ttaccgttaa tattcgcact cattattcct atcctgtata gattttttaa acgtatacat
     3601 ttaggatggt tcgtattacc tgtccctatc gtcatattta tttatatgct tacattaatt
     3661 aaaacgacga tgtcaggtaa tactgtaatg aaaaccttaa attggatgcc tcattttggt
     3721 atgaactttg atttatatct tgatggctta ggtttactct ttagtttgct tatttcagga
     3781 atcggcagtt tagtagtatt atattcaatc ggctacttaa gtaagtcaga acaactaggg
     3841 aatttttatt gttatttatt actatttatg ggtgcaatgt taggtgtcgt actttcagat
     3901 aatgttatta ttctatattt attctgggag ctaacgtcat tttctagttt tttacttatt
     3961 tcattctggc gcgaacgaca agcatctata tatggtgctc aaaaatcgct cataatcaca
     4021 gtatttggtg gtctatcatt attaggtggt atcatcttac tcgctatacc aacacaatca
     4081 tttagcattc agtacatgat tcaacatgct tctgaaattc aaaatagccc attcttcatt
     4141 tttgcgatga ttttaatcat gattggtgct tttacaaaat ctgctcagtt tccattttat
     4201 atttggctac cagatgcaat ggaagcacct acaccagtga gtgcttatct tcattcagca
     4261 acaatggtta aagcagggtt atatttaatc gctcgaatga caccaatctt cgcagcatcg
     4321 caaggttgga tatggactgt cacactcgtt ggtttaatta cgttattctg ggcttcgcta
     4381 aacgcaacaa aacaacaaga cttgaaaggt atactggcat tttcaacagt atcccaatta
     4441 ggtatgatta tggcgatgct tggaatcggt gccattagtt atcactatca aggcgatgat
     4501 agcaaaatat atgctgctgc atttacggct gcaatattcc atttaatcaa ccatgcaaca
     4561 tttaaaggtg ccctatttat gattacaggt gcagtggatc attctactgg tacacgtgat
     4621 gttaagaaat taggtggctt actaacaatt atgcctattt catttacaat cactgttatt
     4681 acagcattaa gtatggctgg tgtaccaccg ttcaatggtt tcttatcaaa agaatcattt
     4741 ttagaaacaa catttacagc aagccaagca aatttattca gtgttgatac attaggctac
     4801 ctattcccaa tcattggtat agtgggcagt gtcttcacat ttgtatattc aattaaattt
     4861 ataatgcata tatttttcgg acaatataaa cctgaacaat tacctaaaaa agcacacgaa
     4921 gtatcaatat taatgctttt atcaccagct attttagcta cattagtaat tgtattcggt
     4981 ttattccctg gcattttaac taattcgatt attgaacctg ctacatcttc gattaatcat
     5041 acggtaattg atgatgttga attccatatg ttccatggat taacgcctgc ctttttatca
     5101 acattagtga tttatatttt aggtatctta ctaattgtga catttagtta ttgggtgaaa
     5161 ttgttgcaac gacaacctgg taaactaaca ttcaattatt ggtacaacag aagtgcaaat
     5221 gttattccaa actactctga aaagatgacg aatagctatg ttaccgatta ttcacgtaat
     5281 aatttagtta tcatttttgg tacattaatt ttattaacat ttgtcactat attctcagta
     5341 cctttcaaca ttaattttaa agacgttagt ccgattagaa ttttcgaagt ttgtatcgta
     5401 attttattat taagtgctgc ctttttaatt cttttcgcta aatcaagatt atttagcatt
     5461 attatgttaa gtgcagttgg atatgctgtg tctgtattgt ttatattctt taaagcaccc
     5521 gacttagcat tgacacagtt tgtcgttgaa tctatttcaa cagcattgtt cttattatgc
     5581 ttctaccact taccaaattt aaatcgttat aatgaaaagc gctcattcca gcttactaat
     5641 gctttaattg ctggtggagt tggtttgtcc gtcattatta tcggtttaat tgcatacgga
     5701 aatagacatt ttgaatcgat atctaagttt tatcaagagc acgtttatga tttagctcac
     5761 ggtaaaaaca tggtaaacgt catacttgtt gatttccgtg gtatggatac tttattcgag
     5821 tcatctgtac ttggtatcgc aggattagct gtatatacaa tgattaaatt acgtaaaaag
     5881 cgtcaaacgc aaggtaacga ggtgaaaaat catgaataga caacaaaatg atctaatttt
     5941 gcaatttgct gcagttatta tctttttcat ggtaatggtc tttggttttt cacttttctt
     6001 agcaggacat tatacacctg gtggcggttt cgtcggcggg ttattattcg caagttcact
     6061 tgttatcatt acgattgcat ttgatataga aacaatgcgt aaaattttcc cgttagattt
     6121 taaaatcttg ataggtatcg gattagtttt ttgtattgcg acgcctatcg caagttggtt
     6181 tttaggcaaa aatttcttta cgcatgtcac atttgatata ccgttattta tattagaacc
     6241 tgttcatatg acaacagcag tattctttga tttcggtgtt ttatgtgcag tagttggtac
     6301 tgtcatgaca attattattt cgattggaga gaacgaatag tggaaattat tatgattttt
     6361 gttagtggta ttctcacagc aattagtgtc tatctcgttt tgtctaaaag tctgatacga
     6421 attgttatgg gaactacact attaacacat gcagcaaatt tatttttaat aactatgggc
     6481 ggacttaaac atggtactgt tccaatttat gaagcgaacg taaaaagcta tgttgatcct
     6541 atcccgcaag cacttatttt aacagcaatc gttatcgcct ttgcgacaac agcctttttc
     6601 ttagtattag catttagaac atataaagaa ttaggcacag ataacgttga gagtatgaaa
     6661 ggagttccag aagatgattg aaagtaatat gcttgtgtta acgctagtta ttccagtaat
     6721 tacagccata ttacttgtct ttattggaaa gcgtcctatt ataaagcgtt atgtcgcact
     6781 aggcggtaca ttattaacct tggtcgcggc aattatcaat ttagcaaatg ttgttaaaca
     6841 tggaccaata cgcgtagagc taggttcatg gaaagcacca tacagtattg tctttgtatt
     6901 agacatattc agtgccctac taataattac tagtattatt attacagcta ttgtgatttt
     6961 atattcatac caaacaattg gtattgaacg tgaaagatat tattattatt tttcagtact
     7021 atttatgtta ataggtatca ttggtgcatt cacaactggt gacattttca acctattcgt
     7081 atttttcgaa gtatttttaa tgtcatctta tttcttatta gtaattggtt ctacaaaaat
     7141 tcaactacag gaaacaataa aatatgttct tgttaacgtt gtatcgtcat ccttcttcgt
     7201 aatgggtgta gcgattttat attcagtagt aggaacattg aatctagcag atataagcaa
     7261 taaattagct aacctttcag cgcatgacag tggcttagtc aatatcgttt tcatactatt
     7321 tatctttgtt tttgctacaa aagctggcgt atttccaatg tttgtttggt tacctagtgc
     7381 atactatgca ccaccaattc caatcattgc attctttggt gctttgttaa caaaagttgg
     7441 tgtttatgca atcgctagaa cattaagcct attctttagt gataatgtaa gcttttcaca
     7501 ttatgtcatt ttattcttag cactattaac gatcatcttt ggttgtgtcg gtgctgttgc
     7561 atatgccaac attaaaaaaa tcatcctata caatgtcatg attgctgtag gtgtgatctt
     7621 agtcggcgtt gcaatgatga cagagtcagg tatgattggg gctatttact atacattgca
     7681 cgacatgtta gtaaaacttg cattattctt gcttatcgga attatgatta aaattacagg
     7741 cactgctgat ttaagacaat ttggtggctt aattaaacga tatcctgtac ttggttggtc
     7801 attctttatc gcagcattaa gtttagctgg cattccccca ctcagtggct tttatggaaa
     7861 atttttcatt gtccaatcaa catttgaaag aggattttat ttaagtggtg tcatcgtctt
     7921 attatcaagt ttagtcgtat tatattctgt cattcgcata ttcctacaag gctttttcgg
     7981 tcaaccaaaa ggatatgatc ttaataacaa agtcgacgtc aaatatttaa ctacgattgc
     8041 aatcgttgca gttgtgataa ctgttttata tggattgtca gcagattact tatatccaat
     8101 ggtaaaagct ggtgcagaaa cattctacaa tccatcaacc tacgttaaag ctgtattagg
     8161 aggtaaataa catggctgta caattagtat taaactttat tatcgcagtg ttttggttgt
     8221 ttgtaacaaa tagttacaca acaaataact ttgtgttagg tttcatcttt ggcctagttc
     8281 ttgtttacct cttacacaga gtgttaccag gtagatttta tgtcatcacg ctatatcgta
     8341 ttattaaatt agttattatt ttcttaatcg aactgattaa agctaatttt gatgttttga
     8401 aaattattat taaaccttcg attaaaaatg aaccaggatt ctttgtttat cacacagatt
     8461 taaaaaaaga ttggcaaatt gtcttgctgt ctaatttaat tacattaaca cctggtactg
     8521 tagtgcttgg tgtaagtgac gatcgtacaa aaatttatat tcatgccata gatttttcaa
     8581 ctaaagagca agaagtagaa agtattaaaa catcattaga gaaaattgta agagaggtgg
     8641 gcgaaatata atgaatcata atgttattat cgttattgca ttaatcatag ttgtcatttc
     8701 tatgttagct atgctcattc gcgttgtgct aggcccatca cttgccgatc gtgttgtcgc
     8761 attagatgcg attggtcttc aattaatggc agttatagca ttattcagta ttttattaaa
     8821 tattaaatac atgattgtcg ttattatgat gattggtata ttagcttttt taggtactgc
     8881 agtattctct aaatttatgg acaaaggtaa ggtgattgaa catgatcaaa atcatactga
     8941 ttagtcttgc acttatcttt gttatcatcg gtgctttaat tagcgcccta gcagctatag
     9001 gattattgag acttgaagat gtatattcac gtgcacatgc tgccggaaaa gcatcaacat
     9061 taggtgcaat gtcattacta tttggtacgt ttttatattt tattgctact caaggttttg
     9121 taaatatgca attaatcgtt gcgattatct ttgtattaat tacaggtcct ctttcaagtc
     9181 atatgattat gaaagcagca tataatatta aaacgcctta tactaaaaag actaaagtcg
     9241 atgaaatttc ggaagactta aaagacacaa aattgtagat tatataacaa aatcatgaat
     9301 ataaatcaac aacaaacagc agtaagatga ttccaaatta ggaatgattt tactgctgtt
     9361 ttcttttgac attgttacct ctttttcaat ggttttttct ttgactacag cttcgcccta
     9421 tctacatata tctctttaat ttaattgcct ttcatgtcgt tatgtattat gataataata
     9481 attataaatc gtaacgatta cgctttaaaa agagagaggt tttattatgc attggacaat
     9541 tatcggcggt ggcatacagg gaactgcaat cgcacaaaaa ctattatcaa gcggattaac
     9601 aacagaccga ttaacaatca ttgacccaca cgaaactttt tgccaaaggt ttaactcatt
     9661 tacaaatcga atagaaatgc cttatttaag atcaccgatt gtacatcacg tacatccaca
     9721 accgttccat ctaaaacaat tcgctaaaca gcaccaatat acaaatgctt tttatggtcc
     9781 ttatcaacga cctgaattga caatgtttat ggatcatatt gcacatgctt ctaaacaata
     9841 tcaattagag gattgcttgg ttcaaggttt agttcaaact ttagataaac aagaagacaa
     9901 atggcatatc aagttagaag atggacaaat tatcactaca gattgcgtcg ttattgcaat
     9961 aggtagtaca aatattccgt ttatgcctga cattttaaaa gacaaacaga atgtaaatca
    10021 tatcttcgag aaagaacatg atcaagtagt atatgataag accgatcata tcgttggtag
    10081 tggcattacg gctgcacatc ttgcacttaa attgttaaat catgataacg ataaaaagat
    10141 tcatttatgg ctaaataaag atattgaaat acatgacttt gatgctgatc ctggttggtt
    10201 aggtccgaag aatatgtcta catttttaag tactaaaagc atgcctgaaa gaaatgccat
    10261 tgtacaacgt gaacgtcata aaggatcaat gcctcacgaa ctgtacttac gccttaaaaa
    10321 acatattaaa aatggtcgta taaatgtgca taaaacacct atcactcaaa ttagtgatgg
    10381 tttaattaac actgaaaatg attctgttcc atatcaacag attatggttg caactggttt
    10441 tgaacaagat tttatgtcac aaccacttat taagcaatta atacaaaatt atgatgcacc
    10501 tattaacgaa tgtaattacc ctgttatttc cgaaaaatta gaatggatac caaatctatt
    10561 tgtcgcagga tgctttgcag acttagaatt aggaccattt ggtagaaatg ttatgggtgg
    10621 ccgtaaagct gccgaacgca ttgaacaagc atttctaaaa ctacaacaat atagcgcata
    10681 aaaaaactgc tagccgtagt tatgaatatc tcataacatt tcagctagca gttcttttat
    10741 tttaaaggtt taatagcaac tgtaccacgc ataactgtaa ttaattgttc tttgtcattc
    10801 ttaattttta tatcccatac atgtgtcgac ttaccttgat gaataatttc agctgtcgca
    10861 gtaacacgac catctttagc agaatgaata tgattggcat tcatctctaa acctaatgga
    10921 ataaatttgg ttgtatcaat tagattagca gatcctaatg aacatgctgt ttcacctaaa
    10981 gcaatcgaag caccaccatg taaatatcca aatggttgtt ttactttatc agtaacaggc
    11041 atagaaataa caactaaacc atcttcctgg tgatctattg acatctcaaa tgtctctaat
    11101 aaatgcgtca tattttcacc tctttatgct tatagtacca tagcagcaat agtaccgaaa
    11161 atcattaaag gaatattata aaacaagaag tttggtacac atgtatcgcg tatatgatca
    11221 tgttggccat caacatttaa tcccgcagtt ggtcctaatg ttgaatcact tgcaggtgaa
    11281 cctgagtcac ctaatgcact cgctgttcca atcaatgcga ttaatgccat tgtatctagt
    11341 ccaattgacg ctccaaaagg aatgaataat gatgcgataa taggaatggt tgcaaatgat
    11401 gatccaatac ctaaagtgac aattaaacct atcacataca tcacgataat gctaaataat
    11461 ttattatcac cagtaatact tgttaaagtt ttaactaatt catctatatc accagtagca
    11521 ttcattacac cagcaaatcc atttgctgtt aaaataacta caccaatata agccataatt
    11581 ttaatacctt caacaaactt agcatctaat tcataccaat tatatgcacg tgaaataaag
    11641 aatacgagta ccccggccag tgcaccaaaa atcattgaat ctgtaaatgt ttgtactaaa
    11701 aatgtagcta gtattgctac aattgttact attaagatat atggttttaa ctctgtaaca
    11761 ttgtcactat ctgaaatttt acgtgtttca tattcacgtg gtttacgata tacatataaa
    11821 ccgataagta agccaacaat ataccccatt gaaggaataa gcattgcttt ccaaatcata
    11881 ttaaactcaa ttgggtgatt cgcctttgca aagccacttt gaataatttg ctggaaaatt
    11941 tgaccgaatc cataaggtaa taacacatac gggaaacata aaccaaaacc gataatcaaa
    12001 ccgattaaac gtctatctat ttttaagtca ttaaacagac ttaacaatgg tgggatgaca
    12061 attggaatga atgcaatatg tacaggaatt aagttttgac tcatgatact catagctaat
    12121 aatgcaataa ttattgtcac ttttacttta actcttgacc atcgactatt ttcagcgtgt
    12181 attgcattta taatttttcc tactaaatag tctgtgatac cactgtatga aattaatgct
    12241 gcaaatccac ctaataaggc atagcttaat gctacctcag caccatcgac tatatttttt
    12301 ccgaatacat taattacttt ttcaatgctc atacctgata ctacgccacc aactagcgca
    12361 ctgataaata agcttataac tacgtttaat cgacataaac atagcacaat cattaaaatt
    12421 actgctatta ctactgcatt tatcatatga caaccatcct ttatcactct atcgaactaa
    12481 agcgtttata gactacatag tatcagtagc tttaacacct gt
//
LOCUS       DAHHOJ010000078        12433 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.078, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000078 DAHHOJ010000000
VERSION     DAHHOJ010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12433
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.078"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..189
                     /locus_tag="NBH32_001864"
     CDS             <1..189
                     /locus_tag="NBH32_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515457.1"
                     /translation="DMFILNTQAHARLDQMAQQFEVVCNGLNENEGQVIQTMDQSASL
                     IRSNLIQVKEQLEKLAVY"
     gene            280..1215
                     /locus_tag="NBH32_001865"
     CDS             280..1215
                     /locus_tag="NBH32_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000476882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1515458.1"
                     /translation="MFKRTKLILIATILLSGCSAMESESKKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFKDNFGLKVKATNVVGKDDGVEVYVH
                     CEDHGIVFNASLPLYKDAIHQKGSMRSNDNGDDMSMMVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTKEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIHKNDK
                     EFKEGMQKARKELNYTANTNTVATLFSTNDERNRKEKINNVIDLSEKIERTKDMPIKN
                     TITTQLGNKLIGTKKARFDDKKVVSFGAFEDEYNK"
     gene            1199..1345
                     /locus_tag="NBH32_001866"
     CDS             1199..1345
                     /locus_tag="NBH32_001866"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515459.1"
                     /translation="MNIINDRDLTELSSYMVYQDINKDNNFTVNEKRFKQADEFEDLY
                     REKL"
     gene            1420..2328
                     /locus_tag="NBH32_001867"
     CDS             1420..2328
                     /locus_tag="NBH32_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1515460.1"
                     /translation="MLKKAKLILIATLLLSGCSAMESESKKDTKAETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHRKEFEKVGEQFFMDNFGLKVKATNVVGKDDGVEVYVH
                     CDDHGIVFNASLPLYKDAIHQKGSMRSNDNGNDMSMVVGTVLSGFEYRAQKEKYDNLY
                     KFFKENEKKYQYTGFTNEAINKTQNVGYQNEYFYITYLSRNLKEYRKYYEPLIRKNDK
                     EFKEGMQRARKELNYTANTDVVSTLFSTKKNFTKDNTVDDVIELSNKLKDKPNMPQKS
                     QVTIQLGKSSINTKKPFYDDINPIEG"
     gene            2783..3727
                     /locus_tag="NBH32_001868"
     CDS             2783..3727
                     /locus_tag="NBH32_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1515461.1"
                     /translation="MLKKAKLILIATLLLSGCSMMKNESSKDTNTETKSVPEEMDASK
                     YVGQGFQPSAEKDAIEFSKKHKDKIAKRGEQFFMDNFGLKVKATNVIGSGDGVEVFVH
                     CDDHDIVFNASIPFDKSIIDSDSSLRSKDKGDDMSTLVGTVLSGFEYRAHKEELDNLT
                     EVLKEYKSKYKYTGYTENAIMKTQNSGFRNEYYYLTAIPYTLDEYKKYFQPLIKEDDK
                     SFRDGMKNSKKQLKDKSRPYIVTTLFSTKDNFTKDNTIDEMIDFSEVLKKKKNIPRDL
                     NVSLQISNKYINTKRPNYSKKDVIEIGVFNHEKANTND"
     regulatory      3933..4067
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            4129..4674
                     /locus_tag="NBH32_001869"
     CDS             4129..4674
                     /locus_tag="NBH32_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCD1515462.1"
                     /translation="MQQNKRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSL
                     LATFTFGPVAGIVVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAIYKNKRSTK
                     SLITGLIIATIVMTIVLSILNYFVLLPLYGMIFNLADIANNLKVIIVSGIIPFNIIKG
                     IVISIVFILLYRRLANFLKRI"
     gene            complement(4780..5028)
                     /locus_tag="NBH32_001870"
     CDS             complement(4780..5028)
                     /locus_tag="NBH32_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002449743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HCD1515463.1"
                     /translation="MAKYTIVDMDTCIACGACGAAAPDIYDYDDEGIAFVILDDNQGT
                     AEVPEELYEDMEDAIDGCPTDSIKIADESFDGDALKFE"
     gene            5136..6089
                     /locus_tag="NBH32_001871"
     CDS             5136..6089
                     /locus_tag="NBH32_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1515464.1"
                     /translation="MQHIIKTALQQTFNYKTNKSIYNILVGKKSHQTFFDACSQQQLS
                     LYHSLPLLKYPSFELFLENITEFNAEMEIMLHPRYIFESMGQTFQAIQLLVQTMSNTM
                     QQDFRFVPISQNNKIQETVKIVYNYIKENQLQIDFENELHNLFKAITLKGPCYLHYYL
                     QGYDEPMYTRQQVSLIEKLSQQQLFEYEMNNLVTMMFELESGEYTILSKIIMKPTLLN
                     QTYITYTRLLEQFTMEDIAAQQQVKINTIEDHVLEILIKGYMSNYDDYVEQEDQLQFL
                     NFYQQHRGERLKFYKEQFDTLSYFQLKVLIVGFERGDLNVA"
     gene            6079..7458
                     /locus_tag="NBH32_001872"
     CDS             6079..7458
                     /locus_tag="NBH32_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCD1515465.1"
                     /translation="MLHDILRNKFGFENFKPGQQEIIESIMSQQHTLGILPTGSGKSL
                     CYQIPTYLSGKPTLIISPLISLMDDQVMQLKINGEKRVTCIHSGMDEIEKKHNIKCLR
                     HSRFIFLSPEFLLQPSNFKLISMIDFGMIVLDEAHCLSEWGYDFRPHYALIGKVTKHF
                     KEAVVLALTATAPPHLQDDLTEMLAIQFKVIKTTMNRPNISFKHLNFHDDEDKIEWLL
                     PFLQQSGPTIIYVSSKKMCLNLAHLIYDSGFLTGIYHGDMNYQERHTVQQQFLNNDIP
                     IIVATSAFGMGINKKDIRTIIHFHLSTSPSNYIQEIGRAGRDGELSQAISLFQPDDKY
                     ILETLLFADMITEEDVQNFEIGEFLAPDKQDVLTTLHSFYSIGALKQIFKQSFKRKQL
                     GFFRMIGYCKLDQCRRKYLLEFFGEYPPAQDRCCDNDSNITDIVILNKKKVIRSIGFD
                     EKLQNLFLR"
     gene            7611..9062
                     /gene="ebpS"
                     /locus_tag="NBH32_001873"
     CDS             7611..9062
                     /gene="ebpS"
                     /locus_tag="NBH32_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elastin-binding protein EbpS"
                     /protein_id="HCD1515466.1"
                     /translation="MSNNFKDDFEKNRQSIDTNSHQDHTEDVEKDQSELEHQDTIENT
                     EQQFPPRNAQRRKRRRDLATNHNKQVHNESQTSEDNVQNEAGTIDDRQVESSHSTESQ
                     EPSHQDSTPQHEEEYYNKNAFAMDKSHPEPIEDNDKHDTIKNAENNTEHSTVSDKSEV
                     EQSQQPKPYFTTGANQSETSKNEHDNDSVKQDQDEPKEHHNSKKAAAIGAGTAGVAGA
                     MAASKAKKHSNDAQNKSNSGKANNSTEDKASQDKSKDHHNGKKGAAIGAGTAGLAGGA
                     ASKSASAASKPHASNNASQNHDEHDHHDRDKERKKGGMAKVLLPLIAAVLIIGALAIF
                     GGMALNNHNNGTKENKIANTNKNNADESKDKDTSKDASKDKSKSTDSDKSKDDQDKAT
                     KDETDNDQNNANQANNQAQNNQNQQQANQNQQQQQQRQGGGQRHTVNGQENLYRIAIQ
                     YYGSGSPENVEKIRRANGLSGNNIRNGQQIVIP"
     gene            9188..9304
                     /locus_tag="NBH32_001874"
     CDS             9188..9304
                     /locus_tag="NBH32_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515467.1"
                     /translation="MLFNKVLSRILTYKLNFSNSHQKDKTLQSTNLTRKNKK"
     gene            9465..10433
                     /locus_tag="NBH32_001875"
     CDS             9465..10433
                     /locus_tag="NBH32_001875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpdA family putative bacillithiol disulfide
                     reductase"
                     /protein_id="HCD1515468.1"
                     /translation="MQKVESIIIGGGPCGLSAAIEQKRKGIDTLIIEKGNVVESIYNY
                     PTHQTFFSSSDKLSIGDVPFIVEESKPRRNQALVYYREVVKHHQLKVNAFEEVLTVKK
                     MNNKFTITTTKDVYECRFLTIATGYYGQHNTLEVEGADLPKVFHYFKEAHPYFDQDVV
                     IIGGKNSAIDAALELEKAGANVTVLYRGGDYSPSIKPWILPNFTALVNHEKIDMEFNA
                     NVTQITEDTVTYEVNGESKTIHNDYVFAMIGYHPDYEFLKSVGIQINTNEFGTAPMYN
                     KETYETNIENCYIAGVIAAGNDANTIFIENGKFHGGIIAQSMLAKK"
     gene            complement(10566..11534)
                     /locus_tag="NBH32_001876"
     CDS             complement(10566..11534)
                     /locus_tag="NBH32_001876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCD1515469.1"
                     /translation="MKHLLVIHTGGTISMSQDQSNKVVTNDINPISMHQDVINQYAQI
                     DELNPFNVPSPHMTIQHVKQLKDIILEAVSNKYYDGFVITHGTDTLEETAFLLDLILG
                     IEQPVVITGAMRSSNEIGSDGLYNYISAIRVASDEKARHKGVMVVFNDEIHTARNVTK
                     THTSNTNTFQSPNHGPLGVLTKDRVQFHHMPYRQQALENVNEKLNVPLVKAYMGMPGD
                     IFSFYSREGIDGMVIEALGQGNIPPSALEGIQQLVSLNIPIVLVSRSFNGIVSPTYAY
                     DGGGYQLAQQGFIFSNGLNGPKARLKLLVALSNNLDKAEIKSYFEL"
     gene            11611..12270
                     /locus_tag="NBH32_001877"
     CDS             11611..12270
                     /locus_tag="NBH32_001877"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCD1515470.1"
                     /translation="MKAINIALDGPAAAGKSTIAKRVASELSMIYVDTGAMYRALTYK
                     YLKLNKTEDFAKLVDQTTLDLTYKADKGQCVILDNEDVTDFLRNNDVTQHVSYVASKE
                     PVRSFAVKKQKELAAEKGIVMDGRDIGTVVLPDADLKVYMIASVEERAERRYKDNQLR
                     GIESNFEDLKRDIEARDQYDMNREISPLRKADDAVTLDTTGKSIEEVTDEILAMVSKI
                     K"
     gene            12301..12417
                     /locus_tag="NBH32_001878"
     CDS             12301..12417
                     /locus_tag="NBH32_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515471.1"
                     /translation="MQRLTHIQNECAENDLLKLKGDFYSMMNESCLFIFGKS"
BASE COUNT         4806 a         1813 c         2153 g         3661 t
ORIGIN      
        1 gatatgttta ttttaaacac acaagctcat gctcgtcttg atcaaatggc acaacagttt
       61 gaagttgttt gtaatggctt gaacgaaaat gaaggacaag ttattcaaac aatggatcaa
      121 tctgcctcgt taattcgttc aaacttaatt caagttaaag aacaattaga aaaactagca
      181 gtttactaat taatttatta aaagctactt gttttcattg agaataagta gctttttcaa
      241 acacaaaagt tttataaaca cataaatggg tgatgagcta tgtttaaaag gacaaaacta
      301 atcttaatag caacaatact gctatcagga tgttcagcga tggaaagtga atcaaaaaaa
      361 gacacgaata cagaaacaaa atcagtacca gaagaaatgg aagcttcaaa atatgtagga
      421 caaggctttc aaccacctgc agaaaaagat gcgattgaat ttgcgaagaa gcatcgtaaa
      481 gaatttgaaa aagtaggtga acaattcttt aaagataact ttggactaaa agttaaagct
      541 acaaatgttg taggtaaaga tgatggtgta gaagtttatg tgcattgtga agatcatggt
      601 attgtattta atgcaagtct acctttgtac aaagatgcca tccatcaaaa aggatcaatg
      661 cgtagtaatg acaacggtga tgatatgagt atgatggtgg gtacagtgct gagtggcttt
      721 gaatatcgag cgcaaaaaga aaagtatgat aatttatata aattttttaa agaaaatgaa
      781 aagaaatatc aatatacagg ttttactaaa gaagcaataa acaagacgca aaacgtcggg
      841 tatcaaaatg aatactttta tattacatat ttatcaagaa acttaaaaga atatcgtaaa
      901 tattacgaac cattgattca taaaaatgat aaagaattta aagaaggtat gcaaaaagct
      961 agaaaagagc taaactatac tgctaataca aatactgtag caacgttatt tagtacgaat
     1021 gatgaaagga atagaaaaga aaagataaat aatgtaatag atttatccga gaaaattgaa
     1081 agaacaaaag atatgccaat caagaatact ataactactc aattaggaaa taaacttatt
     1141 ggcacaaaaa aagctcgttt tgatgataag aaagtagtgt cgtttggagc atttgaagat
     1201 gaatataata aatgatagag atttaacaga attgagtagt tatatggttt atcaagacat
     1261 caataaagat aataacttta cagttaacga aaaacgattt aagcaggcag atgaatttga
     1321 agatttatat agagagaaac tataagacgc aaataaatta agagagtata attatttaca
     1381 aaatgaaact tttaaaagcg cataaatggg tgatgagata tgcttaaaaa ggcgaaatta
     1441 atcttaatag caacgctact gttatcagga tgttcagcga tggaaagtga atcaaaaaaa
     1501 gacacaaaag cagaaacaaa atctgtgcca gaagaaatgg aagcttcaaa atatgtaggt
     1561 caaggtttcc aaccacctgc agaaaaagat gcgattgaat ttgcgaagaa gcatcgtaaa
     1621 gaatttgaaa aagtaggtga acaatttttt atggacaact tcggtctaaa agttaaagct
     1681 accaatgttg taggtaaaga tgatggtgta gaagtttatg tgcattgcga tgatcatggc
     1741 attgtattta atgcaagttt gcccttgtac aaagatgcca tccatcaaaa aggatcaatg
     1801 cgtagtaatg acaatggtaa tgatatgagt atggtggtgg gtacagtgct tagtggcttt
     1861 gaatatcgag cgcaaaaaga aaagtatgac aatttatata aatttttcaa agagaatgaa
     1921 aagaaatatc aatatacagg atttacaaac gaggcaatta acaagacaca aaacgtcggg
     1981 tatcaaaatg aatactttta tattacatat ttatcaagaa acttaaaaga atatcgtaaa
     2041 tattatgaac cattgattcg taaaaatgat aaagaattta aagaaggtat gcaacgagcc
     2101 agaaaagagc taaattatac tgcaaatact gatgtagttt ctacgctttt ttctactaag
     2161 aaaaacttta ctaaagataa tacagtagat gatgtgattg aattgagtaa taaattaaaa
     2221 gataaaccca atatgcctca aaaatctcaa gttactattc aattaggaaa atctagtata
     2281 aatactaaaa aaccatttta tgacgatata aatccaattg aagggtaagt gaatataaag
     2341 tgaataaaat aaatgatagg gacatagcag aattgaacgg ttattgggtt tatcaagata
     2401 taaatgaatg atagatttta aagtaaatga aaaacggttc aaacaaatag aaaatcataa
     2461 tatgaaaggc ataccttaat atagaaatat acgagtttct tgatgatcag gggaatctaa
     2521 cttgtcaaca gataataatc tatcaaggaa cttcaaatga ggcagtaaat cctaataacc
     2581 cattgaaatc tttaggtaat ggagatgatt ggcttcaaaa tgctaaatca acggataatt
     2641 aaataaaaat cagacattgt ttaacgcaag tagatgtatt tgaagattta tatagagaga
     2701 aactataaga cgtaaataaa ttaagagagt ataattattt gcaaaatgag agttttaaaa
     2761 gcgcataaat gggtgatgat atatgcttaa aaaagcaaaa ttaatattaa tagcaacact
     2821 tctactatca ggatgttcca tgatgaaaaa tgaatcaagt aaagacacga atacagaaac
     2881 taaatctgta ccagaagaaa tggatgcttc aaaatatgta ggacaaggat tccaaccatc
     2941 tgcagaaaaa gatgcgattg aattttcgaa gaagcataaa gataaaattg ctaagcgagg
     3001 cgaacaattt tttatggata acttcggtct aaaagttaaa gctacaaatg ttataggtag
     3061 tggcgacggt gtagaagtat tcgtgcattg tgatgaccac gacatcgtat ttaatgcaag
     3121 tattccattt gataaatcaa ttattgacag tgatagttca ttaagaagta aggataaagg
     3181 cgatgatatg agtactttag ttggtacagt gttgagtggc tttgaatatc gagcacataa
     3241 ggaagagttg gataatttaa cagaagtttt aaaagaatat aaaagtaaat ataaatatac
     3301 tggttatact gaaaatgcaa ttatgaaaac gcagaatagt ggttttagaa atgagtatta
     3361 ttatttgact gcaattccat atactttaga tgaatataaa aagtattttc aacccttaat
     3421 caaagaggat gataagtctt ttagggacgg tatgaaaaac tcaaaaaaac aacttaaaga
     3481 taaatctcgt ccatatattg ttacaacttt atttagtact aaagataatt ttactaaaga
     3541 taatacaata gacgagatga ttgatttttc tgaagtacta aaaaagaaaa agaatattcc
     3601 tcgtgatttg aatgtttctc ttcaaatttc taataaatat ataaatacaa aaagacctaa
     3661 ttatagtaag aaggatgtaa tagaaatagg tgtatttaat catgaaaaag ctaacacgaa
     3721 tgattaaact aaaaacaata aaaagatagg ttcaaaaaag ccatcctaac aacagacaaa
     3781 accacaaacc aatcaaacct acccaaccaa tgaactcaaa tcaccaatgc atcactctcc
     3841 gataacgtaa ctatgatctt atttaagcat agttattgag gttttgtgat atatagtata
     3901 aaattaatga gaattaaatt taataatgta aaattcatct tcggggtcgg gtgtaattcc
     3961 caaccggcag taaaaaaagc ctgcgacctg ctagtatgta tcatattagt ggctgatcta
     4021 gtgagattct agagccgaca gtatagtctg gatgggagaa gatggaggtt ttttgttgtg
     4081 caataatcct cctattctta cgagatgaat ggaaggagaa aattgaatat gcaacaaaat
     4141 aaacgtctta tcacaataag tatgttgagc gcgattgcgt ttgtgttaac ttttatcaag
     4201 tttcctatac catttttgcc accatactta actttagatt ttagtgatgt accgtcacta
     4261 ctagctacat ttacgtttgg accagttgcc ggtatcgtag ttgcactggt taaaaattta
     4321 ttgaactact tatttagtat gggcgatcca gttggaccat ttgctaactt tttagcaggc
     4381 gcaagtttct tattaactgc ttacgccatc tataaaaata aacgttcaac aaaatctttg
     4441 attactggat taatcattgc aacaatcgtt atgactatcg tgttgagtat tttgaactat
     4501 ttcgttctat tacctttgta cggtatgata tttaacttag ctgatatcgc aaataatctt
     4561 aaagtaatca ttgtttcagg aattatacca ttcaatatta ttaaaggtat cgttatttct
     4621 attgtattta ttttactata tagaaggctt gcgaatttct tgaaaagaat ttaatcaaat
     4681 taaagcaaaa taatatacac ataataataa aaagcaggtg actatcaata aacgatagct
     4741 tgcctgcttt ttctatggaa catttgtcta aaaaatcaat tattcaaatt ttaaagcgtc
     4801 cccatcaaat gattcgtctg caattttaat agaatctgta gggcatccat caattgcatc
     4861 ttccatatct tcatataatt cctcaggcac ttctgcagta ccttggttat cgtcaaggat
     4921 tacgaaagca ataccttcgt cgtcgtaatc atatatatct ggcgctgctg caccgcatgc
     4981 accacatgca atacaagtat ccatatcaac gattgtatat tttgccaatg tcttcgcctc
     5041 ctttgataaa aatgctaaaa tagtaatgtg actaaaattt tagacagcat catttttatt
     5101 ttcaaattat ccgttttaca gagtgagggt taaacttgca acacattata aaaacagcat
     5161 tacaacaaac atttaactat aaaacaaata aaagtattta caatatctta gttggtaaga
     5221 aatctcacca aacctttttt gacgcttgta gtcaacaaca gttgtcatta tatcacagtt
     5281 taccactatt aaaatatccg tcttttgagc tatttttaga aaatataact gaatttaatg
     5341 ctgaaatgga aatcatgttg catcctagat atatatttga aagtatgggg caaacatttc
     5401 aagcaattca actattagtg caaaccatgt ctaatactat gcagcaagat tttcgtttcg
     5461 taccaatctc tcaaaataat aagatacaag aaactgtaaa gattgtctat aactatatta
     5521 aagaaaacca attacaaatt gattttgaaa atgaactaca taacttgttt aaagcaataa
     5581 ctttaaaagg gccatgttac ttacattatt atttgcaagg ctatgatgaa ccaatgtata
     5641 cgagacagca agttagttta atagaaaagc tatctcaaca gcaattgttt gaatacgaaa
     5701 tgaataattt agtgacaatg atgtttgaat tagaaagtgg agaatatact attttatcaa
     5761 aaataataat gaaacctaca ttattaaacc aaacttatat tacttataca agattgcttg
     5821 aacaattcac gatggaagat atagcggctc aacaacaagt taaaatcaat actatcgaag
     5881 atcatgtact tgaaatctta atcaaaggtt atatgtctaa ttacgatgat tatgttgaac
     5941 aagaagatca actgcaattt ttaaattttt atcaacaaca tcgtggtgaa cgattaaaat
     6001 tttacaaaga acaatttgac acgttatcat attttcaatt aaaagtatta atcgttggat
     6061 ttgaaagagg tgatctgaat gttgcatgat attttacgaa acaaatttgg cttcgagaat
     6121 tttaaaccgg gacaacagga aattatagaa agtataatgt ctcaacaaca cactctaggt
     6181 attcttccaa ctggaagtgg aaagagtttg tgttatcaaa tacctacgta tttatcaggt
     6241 aagccgacat taattatctc accgttaata tctttaatgg atgaccaagt tatgcagttg
     6301 aaaataaatg gagaaaaacg tgtaacatgt attcactctg gtatggatga aattgagaaa
     6361 aagcataata ttaaatgttt acgacatagc cgcttcatct ttctaagtcc agaatttctc
     6421 ctgcaaccgt caaattttaa attaatatct atgatagact ttggcatgat tgttctagat
     6481 gaagcacatt gcctatctga atggggatat gatttcagac cacattatgc tctaatagga
     6541 aaagtaacaa agcattttaa agaagcggtt gtcttagcat tgacagcaac tgcaccaccg
     6601 catttacaag atgatttgac ggaaatgtta gcgattcaat tcaaagttat taaaactaca
     6661 atgaatcgcc caaacataag ctttaagcat cttaattttc atgatgatga agataaaatt
     6721 gaatggttgc tgccgtttct acaacagtcg ggaccaacga ttatttatgt ctcatcgaaa
     6781 aagatgtgtc tgaatttagc gcaccttatt tatgattcag gttttcttac aggcatttat
     6841 catggtgata tgaattatca agagcgacac acagttcaac aacaattttt aaataatgat
     6901 attccgatta tagtcgcaac gagtgctttt ggtatgggaa ttaataaaaa agatattcgc
     6961 acaatcattc actttcatct ttcaacaagt ccttctaact acattcaaga aattggccgt
     7021 gcgggtcgcg atggtgaact aagtcaggca attagtttat ttcaaccgga cgataaatac
     7081 attttagaaa cattattatt tgcagatatg ataacagaag aagatgtaca aaatttcgaa
     7141 ataggagaat ttttagcacc cgataaacaa gacgttttga caacgttgca ttcattctat
     7201 agtatcggcg ccttgaaaca gatatttaag caatcattta aacgaaagca attaggattc
     7261 tttcgcatga ttggctattg caaattagat caatgtagac gaaagtattt attagaattt
     7321 ttcggtgaat atccaccggc acaagatcga tgttgtgaca atgattctaa tataactgat
     7381 atcgtaattt taaataagaa gaaggtaatt agaagtattg gatttgatga aaagttgcaa
     7441 aatttatttc tcagatagta ttactttact aaaagaaaat tgacaagcta taattagtgt
     7501 atacacaatt gaaaaatgat tgaaataatt ttgaaaaata tacataaaca tatgtcatgt
     7561 gggtatattt tatgtaaaat cattgtaata gaatagaaag gaagatggct atgtctaata
     7621 attttaaaga tgactttgaa aaaaatcgtc aatcgataga cacaaattca catcaagacc
     7681 atacggaaga tgttgaaaaa gaccaatcag aattagaaca tcaggataca atagagaata
     7741 cggagcaaca gtttccgcca agaaatgccc aaagaagaaa aagacgccgt gatttagcaa
     7801 cgaatcataa taaacaagtt cacaatgaat cacaaacatc tgaagacaat gttcaaaatg
     7861 aggctggcac aatagatgat cgtcaagttg aatcatcaca cagtactgaa agtcaagaac
     7921 ctagccatca agacagtaca cctcaacatg aagaggaata ttataataag aatgcttttg
     7981 caatggataa atcacatcca gaaccaatcg aagacaatga taaacacgat actattaaaa
     8041 atgcagaaaa taacactgag cattcaacag tttctgataa gagtgaagtt gaacaatctc
     8101 agcaacctaa accatatttt acaacaggtg ctaaccaatc agaaacatca aaaaatgaac
     8161 atgataatga ttctgtaaaa caagatcaag atgaacctaa agaacatcat aatagtaaaa
     8221 aagcagcagc tattggtgct ggaacagcag gtgtagcagg tgcaatggct gcttctaaag
     8281 ctaagaaaca ttcaaatgac gctcaaaaca aaagtaattc tggcaaggcg aataactcga
     8341 ctgaggataa agcgtctcaa gataagtcta aagatcatca taatggcaaa aaaggtgcag
     8401 cgatcggtgc tggaacagca ggtttggctg gaggcgcagc aagtaaaagt gcttctgccg
     8461 cttcaaaacc acatgcctct aataatgcaa gccaaaacca tgatgaacat gaccatcatg
     8521 acagagataa agaacgtaaa aaaggtggca tggccaaagt attgttacca ttaattgcag
     8581 ctgtactaat tatcggtgca ttagcgatat ttggaggcat ggcattaaac aatcataata
     8641 atggtacaaa agaaaataaa atcgcgaata caaataaaaa taatgctgat gaaagtaaag
     8701 acaaagacac atctaaagac gcttctaaag ataaatcaaa atctacagac agtgataaat
     8761 caaaagatga tcaagacaaa gcgactaaag atgaaactga taatgatcaa aacaacgcta
     8821 atcaagcgaa caaccaagca caaaataatc aaaatcaaca acaagctaat caaaatcaac
     8881 aacagcaaca acaacgtcaa ggtggtggcc aaagacatac agtgaatggt caagaaaact
     8941 tataccgtat cgcaattcaa tactacggtt caggttcacc ggaaaatgtt gaaaaaatta
     9001 gacgtgccaa tggtttaagt ggtaacaata ttagaaacgg tcaacaaatc gttattccat
     9061 aatataacta tataaattgt aactgaactg ctatatacaa acgtgcataa tataaagtaa
     9121 ctctaaatat tttagagaac ttaaattgcg cttataagta tgtagcggtt ttttcctttt
     9181 tcaaagtttg ttatttaaca aggtgttgtc tcggatattg acatataaat taaacttttc
     9241 aaatagtcat caaaaagata aaacactaca atcaacaaat ttaacgagga agaataaaaa
     9301 ataaatcaac atattaaatt gtagtgttat tcaactccgt agctaacaat tctctatgca
     9361 cattaaacaa attgtcaaaa atatatcata aatcttcaag cacagactta gcgtatcaat
     9421 cactgaactg ttataatagt ttgggattaa aggaggccga aacaatgcaa aaagttgaaa
     9481 gtatcataat tggtggaggg ccatgcggat taagtgcggc tattgaacaa aaaagaaaag
     9541 gtattgatac cttaattatt gaaaagggta atgtcgttga atcaatctac aattatccta
     9601 ctcaccaaac atttttctca tcaagtgata aattaagtat tggggacgta ccgtttatcg
     9661 ttgaagaaag taaaccaaga cgtaatcaag cgctagttta ttaccgagaa gttgtaaaac
     9721 atcatcaatt aaaagtaaat gcatttgaag aagtattaac tgttaaaaaa atgaataata
     9781 aatttacaat tactacgacg aaagatgttt atgaatgtcg atttttaaca atcgcgacag
     9841 gctattatgg tcagcataat acattagaag ttgaaggtgc ggatttacct aaagtgttcc
     9901 attattttaa agaggcacat ccgtattttg atcaagatgt tgttattatt ggtggtaaga
     9961 attcggctat cgatgctgct ttggagttgg aaaaagctgg tgctaacgtg acggttctat
    10021 atcgtggtgg agattattca ccttcaatta aaccgtggat acttccaaat ttcacagcat
    10081 tagtaaatca tgaaaaaatt gacatggaat ttaatgctaa tgttacccaa ataactgaag
    10141 atactgtgac ttatgaagta aatggtgaaa gtaaaacgat acacaatgat tatgtatttg
    10201 cgatgattgg ttatcatccc gattatgaat ttttaaaatc tgtaggcatt caaattaata
    10261 caaatgaatt tggaacagcg cctatgtata ataaagaaac atacgaaaca aatatcgaaa
    10321 attgctacat tgcaggtgtg attgctgcag ggaatgatgc gaataccatt tttattgaaa
    10381 atggtaaatt ccacggtggc attattgctc aaagcatgct agctaagaaa taaacgccgt
    10441 tagaatcata aaaacaaagg tctatgtact agataactta gttttacaac gactgacatt
    10501 catgatatgt caaatgaggt tgatgactaa ttattgtaca tagacctttt tatattacgt
    10561 aatcattata attcaaaata tgatttgatt tcagctttat ctaaattgtt gcttaacgca
    10621 actaataatt ttaatcttgc ttttggacca ttcaaaccgt tagaaaaaat aaaaccttgt
    10681 tgtgcgagtt ggtaaccacc accatcgtat gcgtaagttg gactcacaat accattaaag
    10741 gaacgtgaaa ctagcacaat aggtatattt aaagatacta attgttgaat gccttctaat
    10801 gcgcttggag gtatgttgcc ttgtcctaac gcttcaataa ccataccatc gataccttca
    10861 cgactataaa aactaaaaat gtcacctggc atacccatat atgcttttac taatggtaca
    10921 tttagtttct cattgacatt ttccaatgct tgttggcgat atggcatatg atggaattgc
    10981 acacgatcct ttgtcaatac acctagcgga ccatgatttg gactttgaaa tgtgtttgta
    11041 ttagacgtat gtgttttggt aacattacgc gccgtatgaa tttcatcatt aaatacaacc
    11101 atcacgcctt tatgacgagc cttttcatca gaggcaacgc gaatagcgga aatataatta
    11161 tataatccgt cagaaccaat ttcattagac gagcgcattg cgccagtaat aacaacaggt
    11221 tgctcgatac ctaatattaa atcaagtaaa aaggcagttt cttctaacgt atcggtacca
    11281 tgcgtgataa cgaaaccatc ataatattta tttgatactg cttctaaaat aatatccttt
    11341 aattgtttaa catgttggat tgtcatatga ggtgatggta cattaaaagg atttaattca
    11401 tctatttgtg catattgatt tatgacatct tgatgcattg aaatagggtt gatatcattt
    11461 gttactactt tattagattg gtcttgtgac atactaatgg tgccaccagt atgaataacc
    11521 agtagatgtt tcatatattt cctcctatat ttcatttacc taattatgat aaaatattat
    11581 tcataaaacg acaaggaagg gaaatgacgc atgaaagcca ttaatattgc attagatggt
    11641 ccagctgctg ccggaaaaag tacaattgcg aaacgtgtag ccagcgaact atcaatgatt
    11701 tatgtcgata caggagcaat gtatcgtgca ttaacataca aatatttaaa attaaacaaa
    11761 actgaggact ttgcaaaact agttgaccaa acaacattag atttaactta taaagcagat
    11821 aaaggtcaat gtgtcatttt agataacgaa gatgtaacag actttttaag aaataatgat
    11881 gtgacgcaac atgtttcata cgttgcatct aaagagccag tacgttcatt cgccgttaaa
    11941 aaacaaaaag agttagctgc agaaaaaggt atcgtaatgg atggtcgcga tatcggaact
    12001 gtagtgctac cagatgcaga tttaaaagta tatatgattg catcagttga agagcgagca
    12061 gaaagaagat ataaagataa tcaattaaga ggtatcgaat caaattttga agatttaaaa
    12121 cgtgatattg aagctcgtga tcaatatgac atgaaccgtg aaatatcacc attaagaaaa
    12181 gcagatgatg cagtgacatt agatacgaca ggcaagtcga ttgaagaagt tactgacgaa
    12241 attttagcga tggtgagtaa aattaaataa tctaataagt cataactatc tatttgtaat
    12301 gtgcaacgct taacgcatat acaaaatgaa tgtgctgaga atgatttact caaattaaaa
    12361 ggtgattttt attcaatgat gaatgaaagt tgccttttta tttttggtaa aagttaatgc
    12421 gtcagtgaat tgt
//
LOCUS       DAHHOJ010000079        12394 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.079, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000079 DAHHOJ010000000
VERSION     DAHHOJ010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12394
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.079"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(150..419)
                     /gene="rpsO"
                     /locus_tag="NBH32_001879"
     CDS             complement(150..419)
                     /gene="rpsO"
                     /locus_tag="NBH32_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCD1515472.1"
                     /translation="MAISQERKNEIIKEYRVHETDTGSPEVQIAVLTAEINAVNEHLR
                     THKKDHHSRRGLLKMVGRRRHLLNYLRSKDIQRYRELIKSLGIRR"
     gene            complement(534..1505)
                     /gene="ribF"
                     /locus_tag="NBH32_001880"
     CDS             complement(534..1505)
                     /gene="ribF"
                     /locus_tag="NBH32_001880"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HCD1515473.1"
                     /translation="MKVIEVTHPIQSKQYITEDVAMAFGFFDGMHKGHDKVFDILNEI
                     AEARSLKKAVMTFDPHPSVVLNPKRKRTTYLTPLSDKIEKISQHDIDYCIVVNFSSRF
                     ANVSVEDFVENYIIKNNVKEVIAGFDFTFGKFGKGNMTVLQEYDAFNTTIVSKQEIEN
                     EKISTTSIRQDLINGELQKANDALGYIYSIKGTVVQGEKRGRTIGFPTANIQPSDDYL
                     LPRKGVYAVSIEIGTENKLYRGVANIGVKPTFHDPNKAEVVIEVNIFDFEDNIYGERV
                     TVNWHHFLRPEIKFDGIDPLVKQMNDDKSRAKYLLAVDFGDEVAYNI"
     gene            complement(1520..2437)
                     /gene="truB"
                     /locus_tag="NBH32_001881"
     CDS             complement(1520..2437)
                     /gene="truB"
                     /locus_tag="NBH32_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCD1515474.1"
                     /translation="MYNGILPVYKERGLTSHDVVFKLRKILKTKKIGHTGTLDPEVAG
                     VLPVCIGNATRVSDYVMDMGKAYEATVSIGRSTTTEDQTGDTLETKGVHSADFNKDDI
                     DRLLESFKGIIEQIPPMYSSVKVNGRKLYEYARNNETVERPKRKVNIKDIGRISELDF
                     KENECHFKIRVICGKGTYIRTLATDIGVKLGFPAHMSKLTRIESGGFVLKDSLTLEQI
                     KELHEQDSLQNKLFPLEYGLKGLPSIKIKDSHIKKRILNGQKFNKNEFDNKIKDQIVF
                     IDDDSEKVLAIYMVHPTKESEIKPKKVFN"
     gene            complement(2606..2956)
                     /gene="rbfA"
                     /locus_tag="NBH32_001882"
     CDS             complement(2606..2956)
                     /gene="rbfA"
                     /locus_tag="NBH32_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCD1515475.1"
                     /translation="MSSMRAERVGEQMKKELMDIINNKVKDPRVGFITITDVVLTNDL
                     SQAKVFLTVLGNDKEVENTFKALDKAKGFIKSELGSRMRLRIMPELMYEYDQSIEYGN
                     KIERMIQDLHKQDR"
     gene            complement(3342..5459)
                     /gene="infB"
                     /locus_tag="NBH32_001883"
     CDS             complement(3342..5459)
                     /gene="infB"
                     /locus_tag="NBH32_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCD1515476.1"
                     /translation="MSKQRIYEYAKELNLKSKEIIDELKSMNIEVSNHMQALEDDQIK
                     ALDKKFKKEQKNDNKQSTQNNHQKSNNQNQNKGQQKDNKKNQQQNNKGNKGNKKNNRN
                     NKKNNKNNKPQNQPAAPKEIPSKVTYQEGITVGEFADKLNVESSEIIKKLFLLGIVAN
                     INQSLNQETIELIADDYGVEVEEEVVINEEDLSIYFEDEKDDPEAIERPAVVTIMGHV
                     DHGKTTLLDSIRHTKVTAGEAGGITQHIGAYQIENDGKKITFLDTPGHAAFTTMRARG
                     AQVTDITILVVAADDGVMPQTIEAINHAKEAEVPIIVAVNKIDKPTSNPDRVMQELTE
                     YGLIPEDWGGETIFVPLSALSGDGIDDLLEMIGLVAEVQELKANPKNRAVGTVIEAEL
                     DKSRGPSASLLVQNGTLNVGDAIVVGNTYGRIRAMVNDLGQRIKTAGPSTPVEITGIN
                     DVPQAGDRFVVFSDEKQARRIGESRHEASIVQQRQESKNVSLDNLFEQMKQGEMKDLN
                     VIIKGDVQGSVEALAASLMKIDVEGVNVRIIHTAVGAINESDVTLANASNGIIIGFNV
                     RPDSGAKRAAEAENVDMRLHRVIYNVIEEIESAMKGLLDPEFEEQVIGQAEVRQTFKV
                     SKVGTIAGCYVTEGKITRNAGVRIIRDGIVQYEGELDTLKRFKDDAKEVAKGYECGIT
                     IENYNDLKEGDVIEAFEMVEIKR"
     gene            complement(5464..5781)
                     /locus_tag="NBH32_001884"
     CDS             complement(5464..5781)
                     /locus_tag="NBH32_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ family RNA-binding protein"
                     /protein_id="HCD1515477.1"
                     /translation="MSIDQILNFLGLAMRAGKVKTGESVIVNEIKKGNLKLVIVANDA
                     SDNTAKLITDKCKSYKVPFRKFGNRNELGIALGKGERVNVGITDPGFAKKLLSMIDEY
                     HKE"
     gene            complement(5778..6062)
                     /locus_tag="NBH32_001885"
     CDS             complement(5778..6062)
                     /locus_tag="NBH32_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HCD1515478.1"
                     /translation="MKKKKIPMRKCILSNEMHPKKDMIRVVVNKEGEIFADVTGKKQG
                     RGAYVSKDVAMVEKAQQKEILEKYFKASKEQLDPVYKEIIRLIYREEIPK"
     gene            complement(6083..7258)
                     /gene="nusA"
                     /locus_tag="NBH32_001886"
     CDS             complement(6083..7258)
                     /gene="nusA"
                     /locus_tag="NBH32_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCD1515479.1"
                     /translation="MSSNELLLATEYLEKEKKIPRAVLIDAIEAALITAYKKNYDSAR
                     NVRVELNMDQGTFKVIARKDVVEEVFDDRDEVDLSTALVKNPAYEIGDIYEEDVTPKD
                     FGRVGAQAAKQAVMQRLRDAEREILFEEFIDKEEDILTGIIDRVDHRYVYVNLGRIEA
                     VLSEAERSPNEKYIPNERIKVYVNKVEQTTKGPQIYVSRSHPGLLKRLFEQEVPEIYD
                     GTVIVKSVAREAGDRSKISVFSENNDIDAVGACVGAKGARVEAVVEELGGEKIDIVQW
                     NEDPKVFVKNALSPSQVLEVIVDETNQSTVVVVPDYQLSLAIGKRGQNARLAAKLTGW
                     KIDIKSETDAREAGIYPVVEAEKVTEEDIALEDADTTESTEEVNDVSVETNVEKESE"
     gene            complement(7279..7746)
                     /gene="rimP"
                     /locus_tag="NBH32_001887"
     CDS             complement(7279..7746)
                     /gene="rimP"
                     /locus_tag="NBH32_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCD1515480.1"
                     /translation="MSKITEQVEVIVQPIMEDLNFELVDVEYVKEGRDHFLRISIDKE
                     GGVDLNDCTLASEKISEAMDANDPIPEMYYLDVASPGAERPIKKEQDFQNAITKPVFV
                     SLYVPIEGEKEWLGILQEVNNETIVVQVKIKARTKDIEIPRDKIAKARHAVMI"
     gene            complement(8034..12344)
                     /locus_tag="NBH32_001888"
     CDS             complement(8034..12344)
                     /locus_tag="NBH32_001888"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HCD1515481.1"
                     /translation="MTEQQKFKVLADQIKISNQLDAEILNSGELTRIDVSNKNRTWEF
                     HITLPQFLAHEDYLLFINAIEQEFKDIANVTCRFTVTNGTNQDEHVIKYFGHCIDQTA
                     LSPKVKGQLKQKKLIMSGKVLKVMVSNDIERNHFDKACNGSLIKAFRNCGFDIDKIIF
                     ETNDNDQEQNLASLEAHIQEEDEQSARLATEKLEKMKAEKAKQQDNNESAVDKCQIGK
                     PIQIENIKPIESIIEEEYKVAIEGVIFDINLKELKSGRHIVEIKVTDYTDSLVLKMFT
                     RKNKDDLEHFKALSVGKWVRAQGRIEEDTFIRDLVMMMSDIEEIKKATKKDKAEEKRV
                     EFHLHTAMSQMDGIPNISAYVKQAADWGHPAIAVTDHNVVQAFPDAHAAAEKHGIKMI
                     YGMEGMLVDDGVPIAYKPQDVVLKDATYVVFDVETTGLSNQYDKIIELAAVKVHNGEI
                     IDKFERFSNPHERLSETIINLTHITDDMLVDAPEIEEVLTEFKEWVGDAIFVAHNASF
                     DMGFIDTGYERLGFGPSTNGVIDTLELSRTINTEYGKHGLNFLAKKYGVELTQHHRAI
                     YDTEATAYIFIKMVQQMKELGVLNHNEINKKLSNEDAYKRARPSHVTLIVQNQQGLKN
                     LFKIVSASLVKYFYRTPRIPRSLLDEYREGLLVGTACDEGELFTAVMQKDQSQVEKIA
                     KYYDFIEIQPPALYQDLIDRELIRDTETLHEIYQRLIHAGDTAGIPVIATGNAHYLFE
                     HDGIARKILIASQPGNPLNRSTLPEAHFRTTDEMLNEFHFLGEEKAHEIVVKNTNELA
                     DRIERVVPIKDELYTPRMEGANEEIRELSYANARKLYGEDLPQIVIDRLEKELKSIIG
                     NGFAVIYLISQRLVKKSLDDGYLVGSRGSVGSSFVATMTEITEVNPLPPHYICPNCKR
                     SEFFNDGSVGSGFDLPDKTCETCGAPLIKEGQDIPFETFLGFKGDKVPDIDLNFSGEY
                     QPNAHNYTKVLFGEDKVFRAGTIGTVAEKTAFGYVKGYLNDQGIHKRGAEIDRLVKGC
                     TGVKRTTGQHPGGIIVVPDYMDIYDFTPIQYPADDQNSAWMTTHFDFHSIHDNVLKLD
                     ILGHDDPTMIRMLQDLSGIDPKTIPVDDKEVMQIFSTPESLGVTENEILCKTGTFGVP
                     EFGTGFVRQMLEDTKPTTFSELVQISGLSHGTDVWLGNAQELIKTGICDLSSVIGCRD
                     DIMVYLMYAGLEPSMAFKIMESVRKGKGLTEEMIETMKENEVPDWYLDSCLKIKYMFP
                     KAHAAAYVLMAVRIAYFKVHHPLYYYASYFTIRASDFDLITMIKDKTSIRNTVKDMYS
                     RYMDLGKKEKDVLTVLEIMNEMAHRGYRMQPISLEKSQAFEFIIEGDTLIPPFISVPG
                     LGENVAKRIVEARDDGPFLSKEDLNKKAGLSQKIIEYLDELGSLPNLPDKAQLSIFDM
                     "
BASE COUNT         3664 a         2454 c         1688 g         4588 t
ORIGIN      
        1 cttatatcat ctttatctca ttgtaacctt attattcact caatattagc ttaacatata
       61 ataaatgtta atatttgtaa aaagcttttt taatataaat taaggttgga acataaatat
      121 gccccaacct caaagacgtt atattaagat taacgacgga taccaagtga tttaattaat
      181 tcacggtaac gttgaatatc tttactacgt aagtagttta ataaatgtct acgacgacct
      241 accattttta ataatccacg acgtgaatgg tggtcttttt tgtgtgtacg taagtgttcg
      301 tttactgcgt tgatttctgc agtaagtaca gcgatttgta cttctggtga accagtatca
      361 gtttcgtgta cacggtattc tttaatgatt tcgtttttac gttcttgtga aattgccata
      421 atcaatttcc tccttaaaat gtttgaatac tccttaatcc gagttaggcg ttggagattc
      481 gacctactaa gaaaaaggtg tggtatagat tgtttatata actatacgca actctagata
      541 ttataagcta cttcatcacc aaaatcaact gctaataaat atttagcacg cgatttatca
      601 tcattcattt gtttaactaa tgggtcgata ccatcaaatt taatctcagg acgtaagaaa
      661 tgatgccaat tcacggtcac tcgttcacca taaatattat cctcaaagtc aaagatattc
      721 acttcgatga caacttctgc tttgttagga tcatgaaatg ttggctttac acctatgtta
      781 gctacccctc gatataattt attttcagtg ccgatttcaa tactaacagc ataaacacct
      841 ttacgaggca acaaataatc atcactaggt tgaatgttag ctgttgggaa gccaatagtt
      901 cttccccttt tttcaccttg cactacagtg cctttaatag aatatatata gcctaaagca
      961 tcattcgctt tttgcaactc accattgatt aaatcttgac gaatagaagt tgtagaaatt
     1021 ttttcatttt caatttcttg tttactcaca attgtcgtat taaacgcatc atattcttga
     1081 agaacagtca tattaccttt tccaaattta ccaaaagtaa aatcaaaacc agcaatgact
     1141 tcttttacat tatttttaat tatataattt tcaacaaaat cttcaacgct cacattagca
     1201 aacctagatg aaaaattaac cactatacaa taatcaatat catgttggct aattttttcg
     1261 attttatctg aaagtggcgt taaatacgtt gttcgttttc ttttaggatt caacacgaca
     1321 gatggatgcg gatcaaatgt catcaccgct ttttttaaac tgcgtgcctc agctatttcg
     1381 tttaatatat caaagacttt gtcatgacct ttatgcatgc catcgaaaaa tccgaatgcc
     1441 attgcaacat cctctgtaat atactgttta gattgtatag gatgtgtcac ttctatgact
     1501 ttcataaatt ctatctcctt taattaaaga cttttttagg tttaatttct gattcttttg
     1561 tagggtgtac catataaatt gctaatactt tttctgaatc atcatcaata aatacaattt
     1621 ggtctttaat tttgttatca aattcatttt tattaaattt ctgcccattt aaaatacgtt
     1681 tttttatgtg cgaatcttta attttaatgc ttggcaaacc ctttaatcca tattctaaag
     1741 gaaacaattt attttgcaat gaatcctgct catgaagttc ttttatttgt tctaatgtaa
     1801 ggctatcttt caacacaaat ccaccagact cgattcgtgt taatttcgac atatgtgccg
     1861 gaaagcctaa tttcacacca atatcagttg ctagcgttct aatatatgta cctttaccac
     1921 agatgacgcg tattttaaaa tgacactcat tttctttaaa atctaattca gatatacgcc
     1981 caatgtcttt aatattaact ttacgctttg gtctttcaac tgtttcatta ttacgcgcat
     2041 attcatataa ttttctacca tttactttga cggatgagta catcggcgga atttgttcaa
     2101 tgataccttt aaaactttct aacaatcggt caatatcgtc cttattaaaa tctgctgagt
     2161 gtacaccttt tgtttccaat gtatcacccg tttgatcttc agtcgttgta cttcttccta
     2221 tcgatacagt tgcttcataa gctttgccca tatccataac ataatcacta actctcgttg
     2281 cattacctat acataccggt aacacgcctg caacttcggg atcaagcgta cccgtgtgac
     2341 ctattttttt agtttttaat attttacgca atttgaatac aacgtcatga cttgttaaac
     2401 cgcgctcttt atatactggt aatatcccat tatacatata gtcaccttct tttttaaaat
     2461 atattttaat agcgtttcaa ctgaatttat tcataaaaaa actggaaaat gttaactatt
     2521 tctacacatc tatgtattaa ctgtttaatg ttaatatacc gatattaaga aattatcaaa
     2581 cattttccag atacctaaca ctaaattatc tatcttgttt gtgtaaatct tgaatcattc
     2641 gttcaatttt attaccatat tcgattgatt gatcatattc atacattaat tccggcataa
     2701 tacgtaatcg cattctagaa cctaattcag acttaatgaa gccttttgct ttatcaagtg
     2761 ctttaaatgt attttctact tctttatcgt tacctaatac agttaaaaat actttagcct
     2821 gcgataaatc atttgttaaa acaacatctg taattgtaat aaaaccaact cgaggatctt
     2881 tgactttatt gttgatgata tccattaatt ccttcttcat ttgttcacca acacgctctg
     2941 ctctcatact gctcattctt gtcacctctt tatctttaag ttcaattgtt atttgcattt
     3001 tttagtgtaa caatgcttaa acaattattt tagctcacac gttattttaa taaattaagc
     3061 ctataaaatc aaataaagtt tgccgtaaga taggcatttc aacagaaaat tttccaataa
     3121 tagtcgacat tatattttgt cattaaaaat cattctaggc caccacaaaa ttttatacaa
     3181 aatcatgtcc atataacgta acttttaata ggtaatctat tctaataaaa aaccaaaagc
     3241 tagtatcaaa tatgctccct ataaagtaga cacttaaaaa gtgcaatatt tatagggagc
     3301 atatcaatct taactatact tttagcttgt aatttattta attaacgctt aatttcaacc
     3361 atttcaaatg cttcgataac atcgccttct ttaaggtcat tgtagttttc aattgtaata
     3421 ccacattcat aaccttttgc aacttcctta gcatcatctt tgaaacgttt aagtgtatct
     3481 aattcgcctt catattgaac aataccatca cgaataatac gtacaccagc atttcgcgta
     3541 attttacctt cagtaacata acatccagca atagtaccaa ctttagaaac tttgaatgtt
     3601 tgacgaactt cagcttgtcc gataacttgt tcttcaaatt ctggatcaag taaacctttc
     3661 atcgctgatt caatttcttc gataacatta taaataactc tgtgtaaacg catatcaaca
     3721 ttttcagctt ctgcagcacg ttttgcacca ctgtctggac gaacattgaa accaatgata
     3781 ataccatttg aggcattagc aagtgtcacg tctgactcat taattgcacc aaccgctgta
     3841 tgaatgatac gaacatttac gccttcaaca tcaattttca ttaatgatgc agctaaagct
     3901 tcaacagaac cttgaacatc acctttaata ataacgttta aatctttcat ttcaccttgt
     3961 ttcatttgtt caaacaggtt atctaatgaa acatttttac tttcttgacg ttgttgtaca
     4021 atgctagctt cgtgtcttga ttcaccaata cgacgagctt gtttttcatc actaaataca
     4081 acaaagcgat ccccagcttg tggcacatca ttaatacctg taatttcaac aggcgttgat
     4141 ggaccagccg ttttgattct ttgacctaag tcattaacca ttgcacggat acggccgtaa
     4201 gtattaccaa ctacaatcgc atcaccaaca tttaatgtac cattttgtac taataatgat
     4261 gcagaaggac cacgtgattt atctaattca gcttcgataa ctgtaccaac agcacggttt
     4321 ttaggatttg ctttaagttc ttgaacttct gcaactaatc ctatcatttc taataaatcg
     4381 tcgataccat caccacttaa tgcagaaagt ggaacgaaaa ttgtttcgcc gccccaatct
     4441 tcaggaatta aaccatattc agttaattct tgcataactc gatcaggatt tgaagttggt
     4501 ttatcaattt tatttactgc aacaataatt ggtacttctg cttctttagc atggttaatt
     4561 gcttcaattg tttgtggcat aacaccatcg tcagctgcaa ctactaaaat tgtaatatct
     4621 gttacttgtg caccacgcgc acgcatcgtt gtaaatgcag catgtcccgg tgtatctaag
     4681 aaagtgattt ttttgccatc gttttcaatt tgatatgcac caatatgttg agtgattccg
     4741 cctgcttcac ctgctgtaac ttttgtatga cgaattgaat ctaataaagt cgttttacca
     4801 tggtcaacat gtcccataat tgttacaact gctggtctct caattgcttc tggatcatct
     4861 ttttcgtctt caaagtagat tgataaatct tcttcattaa tcacaacttc ttcttcaacc
     4921 tcaacgccat aatcatcggc aattaattcg attgtttctt gatttaatga ttgattgata
     4981 ttagcaacaa taccaagtaa gaataatttt ttgataattt cggatgattc aacatttaat
     5041 ttatccgcaa attcgcctac tgtaatacct tcttgatatg tcacttttga tggtatttct
     5101 tttggagcag ctggttgatt ttgtggttta ttattcttgt tatttttctt attatttcta
     5161 ttattctttt tattgccttt gttgccttta ttattttgtt gttgattctt tttgttatct
     5221 ttttgttgcc ctttattttg gttttgattg tttgattttt ggtgattatt ttgagtgctt
     5281 tgtttattgt cgttcttttg ttcttttttg aactttttat ctaatgcttt aatttggtca
     5341 tcttccaaag cttgcatatg atttgaaacc tcaatattca tgctttttaa ctcatctata
     5401 atctctttac tctttagatt taattctttc gcatattcgt aaattctttg tttactcata
     5461 taatcactcc ttatgatatt catctatcat tgatagcaac tttttagcaa agcctgggtc
     5521 agtaatccct acattaacac gctcaccttt tccaagtgct attcccaatt catttcgatt
     5581 tccaaacttt ctgaatggaa ctttgtaact cttacattta tctgtaatta atttagctgt
     5641 attatcagac gcatcatttg caacaataac gagcttcaaa tttccttttt taatctcatt
     5701 aacaatgact gattcacctg tttttacttt accagctctc attgctaatc ctaaaaagtt
     5761 taatatttga tctatactca ttttgggatt tcttctctat aaattaatct aataatttct
     5821 ttgtaaacag gatccaattg ctctttagat gctttaaaat atttttctaa aatttctttt
     5881 tgttgtgctt tttcaaccat agcaacatct ttagaaacat atgcgccacg gccttgtttc
     5941 tttccagtaa catccgcaaa gatttcgcct tctttattaa caacaacacg aatcatatct
     6001 tttttgggat gcatttcatt tgaaagaata cattttcgca tcggaatttt tttctttttc
     6061 atagatactt cactccaacc tattattcag attctttctc tacatttgtt tcaactgaaa
     6121 catcatttac ctcttcggtt gattctgttg tgtcagcatc ttctaaagca atatcttctt
     6181 cagttacttt ttcagcttca actactggat agatacccgc ttcacgcgca tctgtttctg
     6241 atttaatatc aattttccag ccggttaatt tagcagctag acgtgcgttt tgtcctcttt
     6301 taccaatcgc taatgacaat tgataatctg gaacaacaac taccgtagat tgatttgttt
     6361 catcaacaat aacttctaaa acttgagaag ggcttaaagc attttttaca aatacttttg
     6421 gatcttcatt ccattgaacg atgtcgattt tttcgccacc tagctcttca acaacagctt
     6481 caacacgcgc gcctttggca ccaacacatg caccaacagc atctatatca ttgttttcag
     6541 agaagacact aattttagag cgatcgccag cttcacgtgc tactgattta acaattacag
     6601 taccatcgta aatttctgga acttcttgtt caaataaacg ttttaataaa cctggatggc
     6661 tacgagaaac atagatttga ggaccttttg tcgtttgttc cactttgtta acatatactt
     6721 tgatacgttc gttaggaata tatttttcgt taggacttct ttctgcttca gataaaacag
     6781 cttcgatacg acctaaattc acatatacat aacgatggtc aacacggtca ataattccag
     6841 taagtatgtc ttcttctttg tctataaatt cttcaaataa aatttcacgt tcagcatcac
     6901 gaagacgttg cattactgct tgtttcgctg cttgagcacc tacacgacca aaatctttag
     6961 gtgttacatc ttcttcgtat atatcaccaa tttcatatgc agggttttta acaagcgctg
     7021 tacttaaatc cacttcatct ctgtcgtcaa atacttcttc aacaacatct ttacgagcga
     7081 taactttgaa agtaccttga tccatattta attccacacg gacatttctt gcactatcat
     7141 aattcttttt gtatgcagta attaaagctg cttcaatagc atcaattaat actgctctag
     7201 gaatcttctt ttctttttct aaatactcag tagctaataa taattcatta cttgacacgt
     7261 tttttcctcc tcatcacgtt aaatcataac tgcgtgacgt gcttttgcta ttttgtctct
     7321 cggtatctct atatctttcg ttcttgcttt gattttaact tgtactacaa ttgtttcatt
     7381 attgacttct tgtaaaatgc ctaaccattc cttttcacct tcaattggta catataaaga
     7441 aacaaataca ggtttagtta ttgcattttg gaaatcttgt tcttttttaa ttggacgttc
     7501 tgcaccaggt gacgctacgt ctaaataata catttcagga ataggatcat ttgcatccat
     7561 agcttcactt attttttcag aagctagcgt acaatcattt aaatctacgc caccttcttt
     7621 atcaatagag attctaagaa aatgatctct accctctttg acatattcaa cgtctacaag
     7681 ttcaaaattc aagtcttcca ttattggttg aacgatcact tctacttgtt ctgtaatttt
     7741 actcatacag gcctcccttt ttggcaaata gaaaagagcg ggaatctccc actcttctgc
     7801 ctgagttcac taatttttaa gcaacttaat tatagcataa gtttatgctt gaaacaaatg
     7861 acttcactat taatcagaga ttcttgtaaa agtttgttca tttatttcac cattacattc
     7921 gaatagactc gtaagacgct aaaaaaatac aggcataact ttgtgtccag caacaatttt
     7981 gtatttcttc gactatgcca tacgcattaa ataaatagct taattatttc attttacata
     8041 tcgaatatcg aaagttgagc tttatctggt aaattcggta atgagcctaa ctcatctaaa
     8101 tactcaataa ttttctgaga taatccagct tttttgttta aatcttcttt tgataaaaat
     8161 ggtccatcgt cacgagcttc aacaattcgt ttcgcaacgt tttcgccaag cccaggcact
     8221 gatatgaacg gcggaataag tgtatcgcct tcaatgataa attcgaacgc ctgactcttt
     8281 tctaaactaa tcggttgcat tcgataacct cgatgcgcca tttcattcat aatttccaag
     8341 acagttaata cgtctttttc ttttttacct agatccatat agcgagaata catgtctttt
     8401 acagtatttc gaatgcttgt tttatcttta atcatcgtta ttaaatcaaa gtctgacgca
     8461 cgaattgtaa agtaagatgc atagtaataa agtggatgat gtactttgaa atatgcgata
     8521 cgtactgcca ttaaaacgta tgctgctgca tgggctttag ggaacatgta cttaatttta
     8581 agacatgaat ctaaatacca atccggcact tcattttctt tcatcgtttc aatcatttct
     8641 tcagttaaac ctttaccttt acgtactgac tccattattt taaaagccat tgatggttct
     8701 aaaccagcat acattaaata aaccatgata tcatcacgac aaccaattac acttgataaa
     8761 tcacatatac cggttttaat taattcttga gcattgccta accacacatc tgtcccatga
     8821 gataatcctg agatttgaac taattcagaa aatgttgttg gctttgtatc ttctaacatt
     8881 tgacgcacga atcctgtacc gaattctggt actccaaatg tacctgtttt acataaaatt
     8941 tcattttcag taacacccaa actttcaggt gtactaaaaa tctgcataac ttctttatca
     9001 tctacaggta ttgtttttgg atcaattcct gataaatctt gaagcatacg aatcattgtt
     9061 ggatcatcat gtccaagtat atcaagtttt aatacattat catgaataga atggaaatca
     9121 aaatgtgtcg tcatccatgc tgaattttga tcatcggcag gatattgtat cggcgtaaaa
     9181 tcataaatat ccatgtaatc aggtactaca ataatacccc ctggatgctg tccggttgta
     9241 cgtttaacac ctgtacatcc tttaacgagt cgatctattt cagcgcctct tttgtggatg
     9301 ccttgatcat tcaaataacc tttaacataa ccaaaagcag tcttttcagc aacggtacca
     9361 attgtacctg cacggaatac tttatcctca ccaaataata cttttgtgta gttatgggca
     9421 ttcggttgat attcaccact aaagtttaag tcgatatcag gaactttatc tcctttaaat
     9481 cctaaaaatg tttcaaacgg aatatcttgt ccttctttaa taagtggcgc tccacaagtt
     9541 tcacacgtct tatcaggtaa atcgaatcct gatcctactg aaccatcatt gaaaaattca
     9601 ctccttttac agttcggaca aatatagtgt ggcggtaacg ggtttacttc agtaatctca
     9661 gtcattgtcg ctacaaaact agaacctact gaaccacggg aaccaactaa gtatccatca
     9721 tctaatgatt ttttaactaa acgttgcgaa attaagtaaa ttaccgcaaa tccattaccg
     9781 ataatacttt ttaattcttt ttctaatcga tcaattacga tttgaggcag gtcttcacca
     9841 tacaatttac gcgcatttgc ataacttagt tctctaattt cttcgttagc accttccata
     9901 cgcggtgtgt ataattcatc tttaatagga acaacacgtt caattcgatc tgctaattcg
     9961 tttgtatttt tcacaacaat ttcatgcgct ttttcttcac ctaaaaaatg aaactcgttt
    10021 aacatttcat ctgtagttct aaaatgtgct tccggtaaag ttgagcgatt aagtggattg
    10081 ccgggttgtg atgctattaa aattttacgt gcgataccat catgttcaaa caaatagtgt
    10141 gcatttcctg tcgcaataac aggaataccc gctgtgtcac ctgcatgtat taaacgttga
    10201 taaatttcat gtaatgtttc agtatctcta ataagctctc tatcaattaa atcttgataa
    10261 agtgccggtg gttgaatttc aataaaatca taatatttgg caattttttc aacttggctc
    10321 tggtccttct gcataactgc cgtaaataac tcaccttcat cacacgctgt acctaccaat
    10381 aatccctcac gatattcatc taacaatgaa cgtggaattc gaggcgtacg gtagaaatac
    10441 ttcactaatg atgcacttac aattttaaat agatttttaa gaccttgttg gttttgtaca
    10501 attaatgtga catgactagg tcttgcacgt ttatatgcat cttcattact gagttttttg
    10561 ttgatttcgt tatgatttaa tacgcctaat tctttcattt gttgaaccat ttttatgaaa
    10621 atgtaagctg ttgcctctgt atcataaatg gcacggtgat gttgcgttaa ttctacaccg
    10681 tattttttag ctaagaaatt taaaccatgt ttaccatatt cagtattaat cgtacgagat
    10741 aattctaatg tatcgataac accattcgta gatggtccaa atccaagacg ttcatatccc
    10801 gtgtcgatga agcccatatc aaacgaagca ttatgtgcta cgaatatcgc atcgccaacc
    10861 cattctttaa attctgtaag tacttcttca atctcagggg catctactaa catatcatca
    10921 gtaatatgcg tcaaattgat aatcgtttcc gataatcgtt catgcggatt actaaacctt
    10981 tcaaacttat cgatgatttc gccgttatga actttcacag ctgcaagctc gatgatttta
    11041 tcatactgat ttgataaacc agttgtctca acgtcgaata caacataagt agcatctttt
    11101 aatacgacat cttgtggttt gtatgcaatc ggaacaccat catcaactaa cataccttcc
    11161 ataccgtata tcattttaat accatgtttt tccgctgctg cgtgagcatc tggaaatgct
    11221 tgcacaacat tatggtctgt aaccgcaatg gctggatgtc cccagtctgc tgcctgttta
    11281 acatacgcac taatatttgg tataccatcc atttgactca ttgcagtatg caagtggaat
    11341 tctacacgct tttcttcagc cttatctttt tttgtcgctt ttttaatctc ttcaatatca
    11401 gacatcatca taactaaatc tctaataaat gtatcttctt caatacgacc ttgagcccta
    11461 acccatttac caacacttag cgctttaaaa tgttctaaat catctttgtt tttacgagta
    11521 aacattttta aaactaaaga gtccgtatag tcagtcactt taatttctac gatatgacga
    11581 ccacttttaa gttcttttaa gttaatatca aaaatgacac cctctattgc aactttatac
    11641 tcttcctcaa taatagattc aattggttta atattttcaa tttgaatcgg cttaccaatt
    11701 tgacacttat cgacagcact ttcgttgtta tcttgttgtt tcgctttttc agctttcatt
    11761 ttttcaagtt tctctgttgc caatcgtgca ctttgttcgt cttcttcttg tatatgtgct
    11821 tctaaagaag ctaagttttg ttcttgatca ttatcatttg tttcgaatat gattttatcg
    11881 atatcaaaac cacaatttct aaacgctttg ataagacttc cattgcatgc cttatcaaaa
    11941 tgattacgtt caatgtcatt tgataccatt acttttaata cttttccaga cataataagc
    12001 tttttctgtt tcaattgacc tttaactttt ggagataaag ctgtttggtc aatacagtgt
    12061 ccaaagtatt taattacatg ttcatcttga ttcgtgccat ttgttaccgt aaaacgacat
    12121 gtaacgttgg cgatatcttt aaactcttgc tctattgcat ttataaataa taaataatct
    12181 tcatgagcta agaattgtgg taatgtaata tgaaattccc atgttctgtt tttgttagaa
    12241 acatctatac gtgtcagttc acctgaattt aaaatttcag catctaattg atttgaaatt
    12301 ttaatttgat cagcaagcac tttaaatttt tgttgctctg tcattgccaa gacaattacc
    12361 accttaaata gaaattactt ttacgtgttc gaac
//
LOCUS       DAHHOJ010000080        12146 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.080, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000080 DAHHOJ010000000
VERSION     DAHHOJ010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 12146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12146
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.080"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(14..370)
                     /locus_tag="NBH32_001889"
     CDS             complement(14..370)
                     /locus_tag="NBH32_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCD1515482.1"
                     /translation="MKLGMLLIRWMLATSFLVHGTLKFVDLDGTIHFLGSLGLPPTIA
                     VLMSIGEVVGGLALIIGFLTPYVNVFYICVMLGSIFTLKLTRGYVSGYEFEIIHIVMN
                     LACISSYSWKKWLQFY"
     gene            861..1818
                     /locus_tag="NBH32_001890"
                     /pseudo
     CDS             861..1818
                     /locus_tag="NBH32_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001081330.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
     gene            complement(1864..1995)
                     /locus_tag="NBH32_001891"
     CDS             complement(1864..1995)
                     /locus_tag="NBH32_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515483.1"
                     /translation="MRYVTMRQFIKRIVKTILVGYVLKFIRNKLSGKSSHSTNNKHN"
     gene            complement(2059..2274)
                     /locus_tag="NBH32_001892"
     CDS             complement(2059..2274)
                     /locus_tag="NBH32_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000867358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HCD1515484.1"
                     /translation="MKVNKAIYVIVALFLGGLGIHKFYADKFGQGLFHLLFFWTGIPT
                     IISIIHAIFVIFTKKADRDGYIIFPKN"
     gene            2439..2990
                     /locus_tag="NBH32_001893"
     CDS             2439..2990
                     /locus_tag="NBH32_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515485.1"
                     /translation="MIRQARPEDRFDIAKLVYMVWDDMELELVKHLPKDMVLDAIEKS
                     CVDATYRTFYQHILVYEVENKVAGCIISYSGENELKYEKAWALLDLPEEIKQYGTPLP
                     VKEAKDDEYYIETIATFAAYRGRGIATKLLTSLLESNTHVKWSLNCDINNEAALKLYQ
                     KVGFISDGQIELYKHMYHHLIVK"
     gene            complement(3042..3980)
                     /locus_tag="NBH32_001894"
     CDS             complement(3042..3980)
                     /locus_tag="NBH32_001894"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCD1515486.1"
                     /translation="MSNQYQQYSTVKKYWHLMRPHTLTASVVPVLVGTAASKIYFLGS
                     EDHIKISLFIAMLLACLLIQAATNMFNEYYDYKKGLDDHESVGIGGAIVRNGMSPELV
                     LRLAIAFYILAAILGLFLAANSSFWLLPVGLVCMAVGYLYTGGPFPISWTPFGELFSG
                     VFMGMFIIVIAFFIQTGNIQSYVIWLSVPIVITIGLINMANNIRDRVKDKASGRKTLP
                     ILLGKNASLTFMAIMYFIAYAFIVLTIIIKPGGSLFYLLALLSFPMPVKVIRRFKKND
                     TPPTMMPAMAAAGKTNTFFGLLYALGIYISALFAGI"
     gene            4162..5523
                     /locus_tag="NBH32_001895"
     CDS             4162..5523
                     /locus_tag="NBH32_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000190617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCD1515487.1"
                     /translation="MATGVLEDDIVKEIYDSSKEWVSVEVKLSQSLDPSTLFHLTDNE
                     AGDRFYMRLNDNRTSYFGYKAIQLFKNNSKNKQSIFKDWEKLKHNITFIHPQSEKHHL
                     RVVGGFQFSSHKSDDEWREFGLNHFVLPEVLISTDNNGTFLTYTVKRESFTVEALNDL
                     MDLFNNISDIDVDEQIGEITRNEDIYKDDWRQLVVEAIESINNEEKIVLARRRLIKFD
                     KDISIPYILKQAYSKEKNSYIFLLESQDSIFFSQTPEQLIKVNNKILSTKAVAGTIKR
                     SQDEDEDTKNVDAFLKDNKNLIEHRFVVDSILHDIKPYITELHYDKTPKILKNDHLYH
                     LYTEIKAPLKDDSYISLIDHLHPTPALGGYPKEFAMDFIEQKEFGTRGLYGAPVGYID
                     IYDDCEFIVAIRSMLIKKAQATLFAGCGIVKDSDPDSELAETNLKFTPMMNALGVDMN
                     GKS"
     gene            5510..7183
                     /gene="menD"
                     /locus_tag="NBH32_001896"
     CDS             5510..7183
                     /gene="menD"
                     /locus_tag="NBH32_001896"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCD1515488.1"
                     /translation="MGNHKAALTKQVFTFASELYAYGVREVVISPGSRSTPLALAFEA
                     HPNIKTWIHPDERSAAFFAVGLIKGSERPVAILCTSGTAAANYTPAIAESQISRIPLI
                     VLTSDRPHELRSVGAPQAINQVNMFNNYVSYEFDMPIADDSKETIDAIYYQMQIASQY
                     LYGPHKGPIHFNLPFRDPLTPDLNATELLTSEMKILPHYQKSIDASALRHILNKKKGL
                     IIVGDMQHQEVDQILTYSTIYDLPILADPLSHLRKFDHPNVICTYDLLFRSGLDLNVD
                     FVIRVGKPVISKKLNQWLKKTDAFQILVQNNDKIDVFPIAPDISYEISANDFFRSLME
                     DTTINRVSWLEKWQRLEKKGRKEIKCYLEQATDESAFVGELIKKTSEKDALFISNSMP
                     IRDVDNLLLNKNIDVYANRGANGIDGIVSTALGMAVHKRITLLIGDLSFYHDMNGLLM
                     SKLNNIQMNIVLLNNDGGGIFSYLPQKESATDYFERLFGTPTGLDFEYTAKLYQFDFK
                     RFNSVSEFKNATLLSETSTIYELITNREDNFKQHQILYQKLSEMIHDTL"
     gene            7170..7973
                     /gene="menH"
                     /locus_tag="NBH32_001897"
     CDS             7170..7973
                     /gene="menH"
                     /locus_tag="NBH32_001897"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCD1515489.1"
                     /translation="MTHYNYYEANVETNQVLVLLHGFLSDSRTYHNHIDKYTDICHVI
                     TIDLPGHGEDQSSMDETWNFDYITTLLDRILDKYKDKSITLFGYSMGGRVALYYAING
                     HIPISNLILESTSPGIKEEANQLERRLVDDARAKVLDIAGIELFVNDWEKLPLFQSQL
                     ELPVEIQHQIRQQRLSQSPHKMAKALRDYGTGQMPNLWPRLKEIKVPTLILAGECDEK
                     FVQIAKKMANLIPNSKCKLISDTGHTIHVEDSDEFDTMILGFLKEEQND"
     gene            7966..8787
                     /gene="menB"
                     /locus_tag="NBH32_001898"
     CDS             7966..8787
                     /gene="menB"
                     /locus_tag="NBH32_001898"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCD1515490.1"
                     /translation="MTNRQWETLREYDEIKYEFYEGIAKVTINRPEVRNAFTPKTVAE
                     MIDAFSRARDDQNVSVIVLTGEGDLAFCSGGDQKKRGHGGYVGEDQIPRLNVLDLQRL
                     IRIIPKPVIAMVKGYAVGGGNVLNVVCDLTIAADNAIFGQTGPKVGSFDAGYGSGYLA
                     RIVGHKKAREIWYLCRQYNAQEALDMGLVNTVVPLEKVEDETVQWCKEIMKHSPTALR
                     FLKAAMNADTDGLAGLQQMAGDATLLYYTTDEAKEGRDAFKEKRDPDFDQFPKFP"
     gene            complement(9025..9354)
                     /gene="sspC"
                     /locus_tag="NBH32_001899"
     CDS             complement(9025..9354)
                     /gene="sspC"
                     /locus_tag="NBH32_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin B"
                     /protein_id="HCD1515491.1"
                     /translation="MYQLQFINLVYDTTKLTHLEQTNINLFIGNWSNHQLQKSICIRH
                     GDDTSHNQYHILFIDTAHQRIKFSSIDNEEIIYILDYDDTQHILMQTSSKQGIGTSRP
                     IVYERLV"
     gene            complement(9392..10573)
                     /gene="sspB"
                     /locus_tag="NBH32_001900"
     CDS             complement(9392..10573)
                     /gene="sspB"
                     /locus_tag="NBH32_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine protease staphopain B"
                     /protein_id="HCD1515492.1"
                     /translation="MNSSYKSRVFNIISIIMVSMLILSLGAFANNNKAKADSHSKQLE
                     INVKSDKVPQKVKDLAQQQFAGYAKALDKQSNAKTGKYELGEAFKIYKFNGEEDNSYY
                     YPVIKDGKIVYTLTLSPKNKDDLNKSKEDMNYSVKISNFIAKDLDQIKDKNSNITVLT
                     DEKGFYFEEDGKVRLVKATPLPGNVKEKESAKTVSAKLKQELKNTVTPTKVEENEAIQ
                     EDQVQYENTLKNFKIREQQFDNSWCAGFSMAALLNATKNTDTYNAHDIMRTLYPEVSE
                     QDLPNCSTFPNQMIEYGKSQGRDIHYQEGVPSYEQVDQLTKDNVGIMILAQSVSQNPN
                     DPHLGHALAVVGNAKINDQEKLIYWNPWDTELSIQDADSSLLHLSFNRDYNWYGSMIG
                     Y"
     gene            complement(10655..11719)
                     /gene="sspA"
                     /locus_tag="NBH32_001901"
     CDS             complement(10655..11719)
                     /gene="sspA"
                     /locus_tag="NBH32_001901"
                     /EC_number="3.4.21.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Glu-specific serine endopeptidase SspA"
                     /protein_id="HCD1515493.1"
                     /translation="MKGKFLKVSSLFVATLTTATLVSSPAANALSSKAMDNHPQQTQT
                     DKQQTPKIQKGGNLKPLEQRERANVILPNNDRHQITDTTNGHYAPVTYIQVEAPTGTF
                     IASGVVVGKDTLLTNKHVVDATHGDPHALKAFPSAINQDNYPNGGFTAEQITKYSGEG
                     DLAIVKFSPNEQNKHIGEVVKPATMSNNDETQVNQNITVTGYPGDKPVATMWESKGKI
                     TYLKGEAMQYDLSTTGGNSGSPVFNEKNEVIGIHWGGVPNEFNGAVFINENVRNFLKQ
                     NIEDIHFANDDQPNNPDNPDNPNNPDEPNNPDNPNNPDNPNNPDNPNNPDNPNNPDNP
                     NNPDNPDNGDNNNSDNPDAA"
BASE COUNT         4249 a         1707 c         2209 g         3981 t
ORIGIN      
        1 tctcaaataa tagttaataa aattgtagcc atttcttcca gctgtaacta ctaatacaag
       61 ctaagttcat tacgatatgt atgatttcaa attcataacc acttacatat cctcttgtaa
      121 gtttaagtgt gaagattgag cctaacataa cacaaatata aaaaacatta acataaggcg
      181 ttaaaaagcc gataataagt gctaaacctc caacaacttc accaatggac atcaatactg
      241 cgatggtagg tggtaaacct aaagagccta agaagtgaat tgttccatct aaatcaacaa
      301 atttcaaagt accatgcact aagaatgatg ttgctaacat ccatctaatt aaaagcatac
      361 ctaatttcat catgtcacct ccaacataca tttttttctt aaaacatagt ccaaattata
      421 tttgtaaaga agtggaaagt aaagattatt tataaaaata attattaaat atttattaaa
      481 tgttctattg tattgaacaa ttgttgtaga aagatgttta agtcatcagt tactttttgt
      541 atttataaat aaagattagt acagcatagt gcaaagatta tagggaaatg aattaactat
      601 tgcatcgcta tttatgaatc gtaaacacta ttatgaaatt gcaagagtag tatggacaat
      661 tagaatgatg gtttgagata aatgcgtgtt attaaccagt gcgtcatctt caaattagag
      721 gccatttgct gtttataata agaagatttg ataatcaact atgataagaa aaaaataata
      781 acatcctaaa tatatcataa acaaattaaa tgataaaata ataatgtggt tttctattaa
      841 agttgtttgg aggtaaatgt gtgaatagga atatcgttaa attagttgtg tttatgctaa
      901 tcttagttgt agcagtagcg ggttgtggac aaaaagatac tgaagagaaa actgaaatga
      961 cgacaataaa agatgaatta ggaactgaaa aaattaagaa aaatcctaaa cgtgttattg
     1021 tattagaata tagttttgct gattatttag cagcattaga tatgaaacct gttggtattg
     1081 cagatgatgg cagcactaaa aatataacaa agtcagtaag agataagatt ggagcatatg
     1141 aatcggttgg atctagaccg caaccgaata tggaagtaat aagtaaatta aaaccggatt
     1201 tgatcattgc agatgttagc agacataaga aaatcaaatc agaattgagc aaaattgcgc
     1261 cgacaatcat gttagttagc ggtacgggag attataatgc aaatattgaa gcatttaaaa
     1321 cagtcgctaa agcagtaggc aaagagaaag aaggcgagaa gcgtctggaa aagcatgata
     1381 aaataagcgg agattagaaa gaaaattgaa cagagtacgt taaaatctgc atttgcattc
     1441 ggtatctcaa gagcaggtat gtttattaat aatgaagata catttatggg acaattctta
     1501 attaaaatgg gtattcaacc tgaagtcaca aaagacaaaa ctgcgcatgt tggtgaacgc
     1561 aagggtggcc cttatatata tttaaataat gaagagcttg ccaatatcaa tccaaaagtt
     1621 atgattttag ctacgaatgg aaaaacagat aaaaatagaa cgaaattcat tgatcctgca
     1681 gtttggaaat cattaaaagc tgtgaaagat aataaagttt atgacgttga ccgaaataag
     1741 tggttgcaat caagaggtat tatcgcaagt gaaagtatgg cagaagattt agagaaaatt
     1801 gcagaaaaag caaaataaaa atacagcgcc actcggaaat catataagag tggcgctgta
     1861 tttttaatta tgtttgttat ttgttgaatg tgatgattta cctgaaagtt tatttcgaat
     1921 aaattttaat acataaccga caaggattgt tttaacaatt cttttaatga attgacgcat
     1981 cgttacatac ctcatttctc tttatcttat gaactatata cccattcata tatgcttttt
     2041 aaacgtcatt gtcacaattt aatttttagg gaatataata taaccgtctc tgtctgcttt
     2101 tttagtaaaa atgacaaaaa tggcatgtat tattgagatg atggtaggga tacctgtcca
     2161 gaaaaataat aagtgaaaaa gaccttgtcc aaatttatca gcataaaatt tatgaatacc
     2221 taaacctcca agaaataatg caacaataac ataaatggct ttattgactt tcatttgtaa
     2281 atcctcctta actataattc tacttaaatt cgttgtgaaa accaatattt ctaactttag
     2341 aattttcaaa ctttctaaaa ttataagtat atctttttaa aataagctag aatttctata
     2401 taataaatgt taataacgta aaagggaatg atgacatagt gatacgtcaa gcacgtccag
     2461 aggaccgatt tgatattgcg aagttagttt atatggtttg ggatgatatg gaattagaat
     2521 tggtaaagca tctacctaaa gacatggtat tagatgcaat tgaaaaaagc tgtgttgatg
     2581 caacatatcg aactttttat cagcatattt tagtttatga agtagaaaat aaagtagcag
     2641 gttgtattat tagctatagc ggtgaaaatg aattgaaata tgaaaaagca tgggcactac
     2701 ttgacttacc agaagaaata aaacaatatg gcacgccatt acctgtaaaa gaagctaaag
     2761 acgatgagta ttatatagaa acaattgcga catttgcagc atatagaggt agaggcattg
     2821 cgacaaagtt attaacgtca ttacttgaat caaatacaca tgttaaatgg agtttgaatt
     2881 gcgatattaa taatgaagca gcattaaagt tatatcaaaa agtaggtttt atatctgatg
     2941 gacagattga attatacaag cacatgtatc atcatttaat tgttaaataa aaaaactcga
     3001 cagttcgaag taagtcgatt gccgagtagt atcattttat attaaatgcc tgcaaataat
     3061 gcgctaatat aaatacctaa tgcatataat aaaccgaaaa atgtatttgt tttaccagca
     3121 gcagccattg ctggcatcat tgtaggcggt gtatcattct tcttgaaacg tctgataact
     3181 ttaacaggca ttgggaatga taacaacgca agtaagtaaa ataatgagcc accaggttta
     3241 ataatgatcg taagtacaat aaaggcataa gcgataaagt acatgattgc cataaatgtt
     3301 aaagaagcat ttttacctaa tagaatgggt aaagttttac gaccacttgc tttatctttg
     3361 acacggtcgc gaatattgtt agccatatta attaaaccga tagtgattac tataggtaca
     3421 cttaaccaaa ttacatagct ttgaatattg ccagtttgaa taaagaatgc aataacgata
     3481 ataaacatac ccataaatac gcctgagaat aattcaccga aaggcgtcca tgaaataggg
     3541 aaagggccac ctgtatatag gtaaccaaca gccatacata ctaatccaac tggtaataac
     3601 caaaatgaag agttagcagc taaaaacaaa cctaatattg ctgctaagat gtaaaatgca
     3661 atggctaatc gtagcacaag ctctgggctc ataccgttgc gtacaattgc accaccaatg
     3721 cctacagatt catgatcatc aaggcctttt ttataatcat agtattcgtt aaacatatta
     3781 gttgctgctt gaataagtaa gcatgctagt aacatggcaa tgaataggct gattttaata
     3841 tgatcttcgc taccaagaaa atatatttta gatgctgctg taccaactaa aacgggtact
     3901 acggaagcag ttaatgtatg aggacgcatt aaatgccaat atttcttaac tgtagaatat
     3961 tgctgatatt gattactcat tgaatacaaa acctctttaa atcatatttt ttataacaaa
     4021 gatattttaa tagaaagtag cataaaggtc aattgacgtg aaaaattgca agataagttt
     4081 aagcaattct tgtaacacaa ttatccaaaa atgatacaat ataataatga gtgaaaaaag
     4141 acgaagaaag aagtgaaatg gatggctacg ggcgtattag aggacgatat tgtcaaagag
     4201 atatatgaca gctcaaagga atgggtttca gttgaagtga aattatcaca gtcactagac
     4261 ccgagcacat tatttcatct cactgacaat gaggcaggag atcgctttta tatgcgtttg
     4321 aatgataatc gaacgtcata ttttggctac aaagcaattc aattattcaa aaataattct
     4381 aaaaataaac aatctatttt taaagactgg gaaaaattaa aacataacat cacatttata
     4441 catccgcaat ctgagaaaca tcatcttcga gttgttggag ggtttcaatt ttcaagtcat
     4501 aaatcagatg atgaatggcg agagtttgga ctaaatcatt ttgtattacc tgaagtttta
     4561 atttcaactg ataataatgg gacattttta acttatacag ttaaaaggga aagttttact
     4621 gttgaggcat tgaacgattt aatggatttg ttcaacaata tatcggacat agatgtggac
     4681 gagcaaattg gggaaattac tagaaatgaa gatatttata aagatgattg gcgtcaactt
     4741 gtagtagaag ctatagaatc tattaataat gaagaaaaaa ttgtactagc acgtagacgg
     4801 ttaataaagt tcgataaaga tatcagtatt ccatatattc taaagcaagc atattctaaa
     4861 gaaaaaaaca gttatatatt cttgttagaa tcacaagatt ctatattctt ttcacaaaca
     4921 cctgaacaat taataaaggt caataataaa atactatcga ctaaagctgt agcaggtaca
     4981 attaaacgtt cacaagatga ggacgaagat acaaaaaatg ttgacgcatt tttaaaagat
     5041 aataaaaact taattgaaca tcgatttgtt gttgacagta ttttacatga tattaaacct
     5101 tatatcactg aattacatta tgataagacg cctaaaattc taaaaaatga tcatttatat
     5161 cacttataca ctgaaataaa ggcgccactg aaggatgatt cgtatattag tttaattgat
     5221 catttacatc caacacctgc tttaggtggc tatccaaaag aatttgcaat ggattttatt
     5281 gagcagaaag aatttggtac gcgaggatta tatggtgcac cggttggcta tatagatata
     5341 tacgatgatt gtgaatttat tgttgcaatt cggtcaatgc tgattaagaa agcacaagca
     5401 acattatttg cagggtgtgg cattgttaaa gattctgatc cagatagtga attggcagaa
     5461 acgaacctta agttcacacc tatgatgaat gcattaggag tcgatatgaa tgggaaatca
     5521 taaagcagct ttaacgaagc aagtttttac atttgcatct gagttatatg cgtacggcgt
     5581 aagggaagta gttatcagtc cgggatcacg ctcaacgcca cttgcacttg catttgaagc
     5641 acatccaaat attaaaacat ggatacaccc cgatgagcga agtgcagctt tttttgcggt
     5701 aggattaatt aaaggcagtg aaagacctgt cgctatatta tgtacgtcag gtacagcagc
     5761 agcgaattat acgcctgcaa ttgctgaaag ccaaattagt agaattcctt taatcgtttt
     5821 aacaagtgac cgtccgcatg aattaagaag tgtaggcgca ccacaagcga ttaatcaagt
     5881 aaatatgttt aataattatg taagttatga gttcgatatg cctattgcag atgatagtaa
     5941 agagaccatt gatgcaattt attatcaaat gcaaattgct agtcaatatt tatatggacc
     6001 acataaaggg ccaattcatt ttaacttgcc atttagagat ccgttaacac ctgatttgaa
     6061 tgcaacagaa ttgttaactt ctgagatgaa gattttaccg cactatcaaa aaagtataga
     6121 tgcatcggca ttaagacaca ttttaaataa gaaaaaaggt ttaattattg taggggatat
     6181 gcagcaccaa gaagttgatc aaatactgac gtattcaacg atatatgatt tgcctatttt
     6241 agctgatcct ttaagtcatt taagaaaatt tgatcatccg aatgttatct gtacatatga
     6301 tttgctgttt agaagcggct tagacttaaa tgtggatttc gtaattcgtg ttggaaaacc
     6361 agtgatttct aaaaagttga atcaatggtt aaagaaaact gatgcatttc aaatattagt
     6421 gcaaaacaat gataagattg atgtctttcc gatagcccca gatatttcat atgagatttc
     6481 tgcgaatgat ttctttaggt cattaatgga agacacgacc atcaatcgag taagttggtt
     6541 agaaaaatgg caacgcttag agaaaaaagg gcgtaaagaa attaaatgtt atttggaaca
     6601 agctacagat gagagtgcat tcgttggtga attgattaag aaaacatctg aaaaagatgc
     6661 attatttatt agtaatagta tgcctatcag agatgtagat aacttgttat tgaataaaaa
     6721 tatagatgtc tatgcgaatc gtggtgcgaa tggtattgat ggtatcgttt caactgcact
     6781 gggtatggct gtgcataaac gaataacatt attgataggt gatttatcat tttatcatga
     6841 tatgaatgga ctattaatgt caaaattaaa taatattcag atgaatattg tattattgaa
     6901 caacgatggt ggcggtattt tttcatattt accacaaaaa gaaagtgcaa ctgactattt
     6961 tgaacggttg tttggcacac cgacgggatt ggattttgag tatacagcta agttatatca
     7021 attcgatttt aaacgtttta acagtgtttc agaatttaaa aatgccacat tgttatctga
     7081 aacttcgacg atttatgaat tgataacgaa tcgcgaagat aactttaaac agcatcaaat
     7141 tttatatcag aaattgagtg aaatgattca tgacacatta taattactat gaagcaaacg
     7201 ttgagaccaa tcaagtttta gtattactgc atggttttct tagcgacagt cgtacttatc
     7261 ataatcacat cgataaatat actgatatct gtcatgtcat cactatagac ttaccaggac
     7321 atggcgaaga tcagtcttca atggatgaaa cgtggaattt tgattatatt acgacgttgt
     7381 tagaccgaat tttagataaa tataaagata aatcaataac attgtttgga tattcaatgg
     7441 gtgggcgtgt tgcgttatat tatgcaatta atggtcacat ccctatatct aatttgatat
     7501 tagaaagtac gtcaccaggt attaaagaag aagcaaatca attggaacgc cgtcttgttg
     7561 atgatgcacg tgctaaagta ttagacatag caggtattga attatttgtt aatgattggg
     7621 aaaagttgcc attatttcaa tcgcaactag aattaccagt tgaaatacaa catcaaataa
     7681 gacaacaacg attgtctcaa tcgccacata aaatggccaa agcattaaga gattatggta
     7741 caggtcaaat gccaaactta tggccgcgcc tgaaagaaat taaagtacca acattaatat
     7801 tagctggaga atgtgatgaa aaatttgtac agattgcgaa aaaaatggca aatttaattc
     7861 ctaatagtaa atgtaaatta atttctgata caggtcatac aattcatgtg gaagatagtg
     7921 atgaatttga tacaatgata ttaggatttt taaaggagga gcaaaatgac taacagacaa
     7981 tgggaaacac ttagagaata tgatgaaatc aaatatgaat tttacgaagg gattgctaag
     8041 gtaacaataa atcgccctga agtacgcaat gcgtttacac ctaaaacagt tgctgaaatg
     8101 attgacgcat tttcacgtgc acgtgatgat caaaacgttt cagttatcgt attaactggt
     8161 gaaggtgatt tagcattctg ttctggtggt gaccagaaga aacgtggaca tggtggttat
     8221 gtaggtgaag accaaatccc tcgcttaaat gtattagatt tacaacgttt aattcgtatt
     8281 attccaaaac cggttatcgc gatggtaaaa ggttatgctg taggtggcgg taatgtacta
     8341 aatgttgttt gtgacttaac gattgctgct gataatgcta tttttggaca aactggtcct
     8401 aaagtaggtt catttgatgc gggttatggt tcaggatatt tagcacgtat cgttggacat
     8461 aagaaagcac gtgaaatttg gtacttatgt cgtcaataca atgcacaaga agctttagat
     8521 atgggtctag taaatacagt ggtaccttta gagaaagttg aagatgaaac tgtgcaatgg
     8581 tgtaaagaga ttatgaaaca ctcaccaaca gctttacgat tccttaaagc agctatgaat
     8641 gctgacacag atggtttagc tggtttacaa caaatggctg gggatgcaac attgctttat
     8701 tacacaactg atgaagcgaa agaaggccgt gatgcgttta aagaaaaacg tgatcctgac
     8761 ttcgatcaat tccctaaatt cccataagtt attttgaata tgatattaag tcacttgcgt
     8821 cgtttattag cgacgatagg tggctttttt attttataag aatttaaaag ggaattttaa
     8881 ttaattatca taaagataag gtagtttgag taaattaata gagaaagaat aaatgttaaa
     8941 gattattatt acaatgaaaa aacgagctgg tgagaaattg aattctcgcc aacccgcttt
     9001 atgaagtgaa atcatttaaa ttagttatac taagcgctca taaacgattg gtcgcgaagt
     9061 gccaatacct tgtttggatg acgtttgcat gaggatatgc tgtgtatcat cataatcaag
     9121 aatataaatg atttcttcat tatcaataga tgaaaattta atgcgttgat gtgccgtatc
     9181 tataaaaaga atatgatatt gattgtgact tgtatcatcg ccatgacgta tacaaattga
     9241 tttttgtaat tgatgattac tccaattacc aatgaataaa ttgatattgg tttgttctag
     9301 atgtgtgagt ttggttgtgt cgtaaactaa atttataaat tgtagttgat acatagtttt
     9361 acctgccttt aatcaatatc tatattactt tttagtaacc tatcattgaa ccataccagt
     9421 tataatcacg attgaatgat aaatgtaata ggcttgaatc tgcatcttgg attgataatt
     9481 ctgtatccca aggattccag taaataagtt tttcttggtc attaatttta gcattaccaa
     9541 caactgctag cgcatgtcct aaatgtgggt cattagggtt ttgagataca ctttgtgcaa
     9601 ggatcataat tcctacatta tcttttgtaa gttgatcaac ttgttcatat gatggtacgc
     9661 cttcttgata atgaatatct ctgccttgtg atttaccgta ttcaatcatt tgattaggga
     9721 atgttgagca attaggaagg tcttgttcac ttacttctgg gtataatgta cgcataatat
     9781 catgtgcatt ataagtgtct gtatttttag ttgcatttaa taatgctgcc atactgaaac
     9841 ctgcacacca tgagttatcg aattgttgtt ctctaatttt gaagtttttt aatgtatttt
     9901 catattgaac ttgatcttct tgtatcgctt cgttttcttc aactttagta ggtgttactg
     9961 tattttttaa ctcttgtttc aattttgctg aaactgtttt agcactttct ttttctttta
    10021 cattaccagg tagtggcgta gcttttacta atctaacttt gccatcttct tcaaaataaa
    10081 accctttttc atcagtaaga acagtgatat ttgaattttt atctttaatt tggtctaaat
    10141 ctttagcgat gaagtttgaa attttaacac tgtaattcat gtcttctttt gatttgttta
    10201 aatcatcttt atttttaggg ctaagtgtta aagtataaac aattttaccg tcttttataa
    10261 ctggataata gtaactatta tcttcttcac cattaaattt ataaatttta aaagcttcgc
    10321 caagttcata tttaccagtt tttgcattac tttgtttatc taatgcttta gcataaccag
    10381 caaattgttg ttgtgctaga tcttttactt tttgaggtac tttgtcactc ttaacattaa
    10441 tttctagctg tttagagtgt gaatcggctt tggctttatt gttattagca aatgcgccta
    10501 gtgataaaat aagcattgaa accattatga tgcttataat attgaatact ctagatttat
    10561 atgaactatt catataatac cctccattta aatcaaaata ttttgcgttg ctaagacttt
    10621 gcttatattt tatctagacg aacttaattg ttaattaagc tgcatctgga ttgtctgaat
    10681 tattgttatc gccattgtct ggattatcag ggttattagg gttgtcaggg ttattaggat
    10741 tgtccggatt attaggattg tccggattat taggattgtc cggattatta ggattgtctg
    10801 ggttatttgg ttcatctggg ttgttagggt tatcaggatt atctgggtta ttaggttggt
    10861 catcgttggc aaaatggata tcttcaatat tttgttttaa gaagttgcgt acattttcat
    10921 taataaatac cgcaccatta aattcatttg gtacaccgcc ccaatgaatt ccgattactt
    10981 catttttttc attaaataca ggtgaacctg agttaccacc agttgtactt aaatcatatt
    11041 gcatagcttc gcctttgagg taagtgattt ttcctttact ttcccacatt gttgctacag
    11101 gtttatcacc aggatatcct gttacagtaa tattttggtt aacttgtgtt tcatcattat
    11161 tactcattgt tgctggttta actacttcgc ctatatgttt gttttgctca ttaggggaga
    11221 atttaacgat tgctaaatca ccttcgcctg aatatttagt gatttgttca gcagtgaaac
    11281 caccatttgg ataattgtct tggttaattg cagaagggaa tgcttttaaa gcatgaggat
    11341 caccgtgcgt agcatctacg acgtgtttat ttgttaaaag tgtatcttta cctacaacta
    11401 caccggaagc aataaatgta ccagtaggtg cttcaacttg aatataagta acaggtgcat
    11461 aatgaccatt cgttgtatct gtgatttggt gacgatcgtt atttggtaat ataacattag
    11521 cgcgttcacg ttgttctaat ggtttaaggt taccgccttt ttgaatctta ggtgtttgct
    11581 gtttgtctgt ctgcgtttgt tgtggatgat tgtccatagc ttttgaagat aacgcatttg
    11641 ctgctggaga actcacaagt gtcgctgttg tcaaagttgc aacgaataaa gaactaactt
    11701 ttaaaaattt acctttcatc taaaaacctc caaaaaattt atttacaagt taaatataac
    11761 actaaaattt tttaagtcaa taagaatata taaataaatt aaaaagtttt tgtaaagatt
    11821 agagtttaca aaaacattaa gggaattaaa attgaattgt attatacttt attcgattgt
    11881 gttaaattga taaggttgtc aattgtttgc atttatctaa aagaataaat aggtaagcaa
    11941 ttgaggacta agggaaagta tttgattata ataataaata gtaacttttg aataataatt
    12001 gtttagccaa aggtagattt atatgaatgg tgatatatta tttgagtgct taagtttcga
    12061 agaacaatta taagttagca tattaaaacc ttttgataga atcattttga gtgaggtgtt
    12121 taaaggagag gaatgtaact cggtag
//
LOCUS       DAHHOJ010000081        11564 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.081, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000081 DAHHOJ010000000
VERSION     DAHHOJ010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11564
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.081"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            51..977
                     /locus_tag="NBH32_001902"
     CDS             51..977
                     /locus_tag="NBH32_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515494.1"
                     /translation="MGKYIFKRFIYMLISLFIIITITFFLMKLMPGSPFNDAKLNAEQ
                     KEILNEKYGLNDPVATQYLHYLKNVVTGDFGNSFQYHNQPVWDLIKPRLLPSFEMGLT
                     AMFIGVILGLILGVAAATKQNSWVDYTTTVISVIAVSVPSFVLAVLLQYVFAVKLRWF
                     PVAGWEGFSTAVLPSLALSAAVLATVSRYIRAEMIEVLSSDYILLARAKGNSTMRVLF
                     GHALRNALIPIITIIVPMLASILTGTLTIENIFGVPGLGDQFVRSITTNDFSVIMAIT
                     LLFSTLFIVSIFIVDILYGVIDPRIRVQGGKK"
     gene            977..2047
                     /locus_tag="NBH32_001903"
     CDS             977..2047
                     /locus_tag="NBH32_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515495.1"
                     /translation="MAENKNNLSINEDHSNAAMTHTSDAIASSDFIIRELDFNQEPEM
                     QRESKNFWQDAWAQLKRNKLAVVGMIGLIIIVIFAFIGPVINKHDYAEQNVQHRNLPA
                     KIPVLDKVPFLPFDGKDADGKDAYKAANAKENYWFGTDQLGRDLWTRTWKGAQISLFI
                     GVVAAMLDIFIGVVYGAISGFFGGRVDTIMQRILEVIASIPNLIVVILFVLIFEPSIW
                     TIILAMSITGWLGMSRVVRGEFLKLKNQEFVMASKTLGASKFKLIFKHILPNTLGAIV
                     VTSMFTVPSAIFFEAFLSFIGIGVPAPQTSLGSLVNDGRAMLLIYPHELFIPAMILSL
                     LILFFYLFSDGLRDAFDPKMRK"
     gene            2063..3145
                     /locus_tag="NBH32_001904"
     CDS             2063..3145
                     /locus_tag="NBH32_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515496.1"
                     /translation="MTERILEVNDLHVSFDITAGEVQAVRGVDFYLNKGETLAIVGES
                     GSGKSVTTKAITKLFQGDTGRIKKGEILFLEEDLAKKPENELIKLRGKDISMIFQDPM
                     TSLNPTMQIGKQVMEPLIKHKNYSKAQAKKRALEILNLVGLPNAEKRFKAYPHQFSGG
                     QRQRIVIATALACEPKVLIADEPTTALDVTMQAQILDLMKELQQKIDTAIIFITHDLG
                     VVANIADRVAVMYGGQMVETGDVNEIFYDPKHPYTWGLLSSMPDLSTTNDTPLLAIPG
                     APPDLLHPPKGDAFARRSQYALDIDFKVEPPWFKVSPTHFVKSWLLDARAPKVELPEL
                     VKQCMKPMPNNYEKPLKVERVSFNEK"
     gene            3135..4076
                     /locus_tag="NBH32_001905"
     CDS             3135..4076
                     /locus_tag="NBH32_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515497.1"
                     /translation="MKNDEVLLSIKNLKQYFNAGKKNEVRAIENISFELYKGETLGLV
                     GESGCGKSTTGKSIIKLNDITSGEILYEGIDIQKIRKRKDLLKFNKKIQMIFQDPYES
                     LNPRLKVMDIVAEGIDIHHLATDKRDRKKRVYDLLETVGLSKEHANRYPHEFSGGQRQ
                     RIGIARALAVEPEFIIADEPISALDVSIQAQVVNLLLKLQREKGITFLFIAHDLSMVK
                     YISDRIAVMHFGKIVEIGPADDIYQYPLHDYTKSLLSAIPQPDPESERSRKRFSYIDD
                     EANNHLRQLHEIRPQHFVFSTEEEAAQLRENKLVTQN"
     gene            4094..5749
                     /locus_tag="NBH32_001906"
     CDS             4094..5749
                     /locus_tag="NBH32_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1515498.1"
                     /translation="MTRKFRTLILILIATIALSGCANDDGIYSDKGQVFRKILSSDLT
                     SLDTSLITDEISSEVTAQTFEGLYTLGKGDKPVLGVAKAFPEKSKDGKTIKVKLRSDA
                     KWSNGDKVTAQDFVYAWRKTVDPKTGSEFAYIMGDIKNASDISTGKKPVEQLGIKALN
                     DETLQIELEKPVPYINQLLALNTFAPQNEKVAKKYGKNYGTAADRAVYNGPFKVDDWK
                     QEDKTLLSKNQYYWDKKNVKLDKVNYKVIKDLQAGASLYDTESVDDAVITADQVNKYK
                     DNKGLNFVLTTGTFFVKMNEKQYPDFKNKNLRLAIAQAIDKKGYVDSVKNNGSIPSDT
                     LTAKGIAKAPNGKDYASTMNSPLKYNPKEARAHWEKAKKELGKNEVTFSMNTEDTPDA
                     KISAEYIKSQVEKNLPGVTLKIKQLPFKQRVSLELSNNFEASLSGWSADYPDPMAYLE
                     TMTTGSAQNNTDWGNKEYDQLLKVARTKLALQPNERYENLKKAEEMFLGDAPVAPIYQ
                     KGVAHLTNPQVKGLIYHKFGPNNSLKHVYIDKSIDKETGKKKK"
     gene            5960..7675
                     /locus_tag="NBH32_001907"
     CDS             5960..7675
                     /locus_tag="NBH32_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1515499.1"
                     /translation="MGKLIKYISILIVIVLVLSACGKSSNKDEGVKDATKTETSKHKG
                     GTLNVALTAPPSGVYSSLLNSTHADAVVEGYFNESLLATDKKIRPKAYIASWKDIEPA
                     KKIEFKIKKGIKWHDGNELKIDDWIYSIEVLANKDYEGAYYPSVENIQGAKDYHEGKT
                     DHISGLKKIDDYTMQVTFDKKQENYLTGFITGPLLSKKYLSDVPIKDLAKSDKIRKYP
                     IGIGPYKVKKIVPGEAVQLVKFDDYWQGKPALDKINLKVIDQAQIIKAMEKGDIDVAN
                     DATGAMAKDAKSSNAGLKVLSAPSLDYGLIGFVSHDYDKKSNKTGKVRPKYEDKELRK
                     AMLYAIDREKWIKAFFNGYASEINSFVPSMHWIAADPKELNDYKYDPEKAKKILDKLG
                     YKDRDGDGFREDPKGNKFEINFKHYSGSNPTFEPRTAAIKDFWEKVGLKTNVKLVEFG
                     KYNEDLANASKDMEVYFRSWAGGTDPDPSDLYHTDRPQNEMRTVLPKSDQYLDDALDF
                     EKVGIDEKKRKDIYVKWQKYMNDELPGLPMFQGKSITIVNDKVRNLDIEIGTDQSLYN
                     LTKEA"
     gene            7726..8712
                     /locus_tag="NBH32_001908"
     CDS             7726..8712
                     /locus_tag="NBH32_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515500.1"
                     /translation="MNNVLLEVKDLETSLKINNEWLATVENISFELSKGEVLGIVGES
                     GCGKSILSKSIIKLLPEKISKLSSGEVIFDGKRIDTLNEKQLLDIRGNDIAMIFQEPM
                     TALNPVFTIKNQLVESIKSHKKISKKEANNLAKDLLKKVGIARQDEILNSYPHQLSGG
                     MRQRVMIAMAISCSPKLLIADEPTTALDVTIQAQILDLLKELQKETQMAIMMITHDLS
                     VVAEFCDKVLVMYAGQIVEFGGIKEILHNPKHPYTQKLLSTIPKLKEEQKRLETIEGI
                     VPSIQAFHVNKCRFANRCNKKMDICNNQSPKMHVCEDVIVRCHLYKNEYKEI"
     gene            8715..9695
                     /locus_tag="NBH32_001909"
     CDS             8715..9695
                     /locus_tag="NBH32_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000427769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCD1515501.1"
                     /translation="MENILEVNQIKKYYKIKTGLLQKTQYVKAVDDVSFSIKKGQTFG
                     LVGESGCGKSTLGKVIIRLEDATSGSIIVNGEDITRLQGKKLRKSRQQYQMIFQDPYA
                     SLNPMQMVGDIISEPILNYKKLPKEEIKKEVLYLLKCVGLSEDAYYKYAHEFSGGQRQ
                     RVGIARALALRPSLIVADEPVSALDVSVQSQVLNLLKDLQEQFNLSYLFIAHDLSVVK
                     HISDVIGVMYLGHIVEIASDKEIYENPKHPYTKALISSIPQIDKHNNNRIILKGELPS
                     PSNPPQGCPFHTRCPIAKDKCKENIPQLKDIGDEHQVACFYVNKVGDLNG"
     gene            9688..10650
                     /locus_tag="NBH32_001910"
     CDS             9688..10650
                     /locus_tag="NBH32_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515502.1"
                     /translation="MVKLILKRLGLMIPLLILISIVVFSLAIIQPGDPFSDLQNGKIK
                     QEAINAQREKLGLNDSISHQYIRWVNHVIHGDLGESIKYKRPVIDVIEERIPNTILLG
                     AMSLIITYIISFALGITSGRYSYSLTDYTVQIFNYLMLAIPSFIAGVFAIFIFSFELQ
                     WFPFQGSVDINLKEGTFEYYMSKIYHTFLPALTLGLLSTAGYIQYLRNDIIENSKKYY
                     VLTARSKGLSMNKIYNKHILRNSLIPIITFLGADIVSILGGAVITETIFSYNGIGKLF
                     LESVIGQDYPLMMALTLFFSFLGLLGNLISDITYGFIDPRIRSN"
     gene            10662..11543
                     /locus_tag="NBH32_001911"
     CDS             10662..11543
                     /locus_tag="NBH32_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515503.1"
                     /translation="MQNKSKSPFKIALSKFTHNKIAMLSIIFLLIITIVSIIAPLIAP
                     FPVNQQDLLNIKGEMTAQNILGTDSGGRDNFSRLLYAGRISLSIGITSTIGMLLIGIT
                     VGVISGYFGSIVDTLLMRITEFVMLFPFLIFAIVLNAALGDKIKNPYGSAIILVLVII
                     VLSWGGIARLVRGKVLQEKENEYFLAAKSIGTPTYKIILKHLLPNILSVVIVQATLLF
                     AGMIVVESGLSFLGFGISKAIPSWGNMLSDAQEGDVISGKPWIWMPPAIMITLTILSI
                     NFVGEGLKDAFNPRGRR"
BASE COUNT         4242 a         1546 c         2157 g         3619 t
ORIGIN      
        1 tgaagggaat ggaaatttag aaatcggaat ttgttagagg aggggattag atggggaaat
       61 atattttcaa acgatttatt tatatgctta tttctttatt tattattatt acaattacat
      121 ttttcttaat gaaattaatg ccaggttcgc catttaacga tgctaaatta aatgctgaac
      181 aaaaagaaat tttaaatgaa aaatatggat taaatgatcc tgtagctacg cagtatttac
      241 attatttaaa aaatgttgtt acaggcgatt ttggtaattc attccagtat cataatcaac
      301 ctgtgtggga tttgattaaa ccgagactac taccttcttt tgaaatgggt cttacagcaa
      361 tgttcatcgg tgtgatactg ggacttattt taggtgttgc agcagctact aaacaaaatt
      421 cttgggttga ctatacaact acagttattt cagttattgc agtatctgta ccatcttttg
      481 tacttgctgt acttttacaa tatgtatttg cagttaaatt aagatggttc ccagtagctg
      541 gatgggaagg tttttcgacc gcggtattac cgtcacttgc attatctgca gctgttttag
      601 caactgtctc cagatacata agagcagaga tgattgaggt attaagttca gactacattt
      661 tattagcgag agctaaaggt aattcgacaa tgcgtgtact ttttggacat gcacttagaa
      721 atgctttaat tccaattatt acaattatcg ttcccatgtt agcaagtatt ttaacaggca
      781 ctttaactat tgaaaatatt tttggggttc ctggattagg ggatcaattc gtacgttcaa
      841 ttacaacaaa tgatttttca gtaatcatgg caatcacact attatttagc acactgttta
      901 tcgtttctat ttttattgta gatattttgt acggtgtgat agatccacga attcgtgttc
      961 aaggaggtaa aaaataatgg ctgaaaataa aaacaatttg tcgattaacg aagatcattc
     1021 taatgcagct atgacgcata cctctgacgc tatcgcatca tctgatttta ttattagaga
     1081 attagatttt aatcaggaac ctgaaatgca acgagaaagc aaaaactttt ggcaagatgc
     1141 ttgggctcaa ttaaaacgaa ataagttagc tgttgtcggt atgataggtt taattatcat
     1201 tgtaatattt gcttttatcg gtccagttat aaataaacat gattatgctg aacaaaatgt
     1261 acaacataga aatcttccgg caaaaatacc tgtattagac aaagttccat ttttaccttt
     1321 tgatggtaaa gatgcagatg gcaaggatgc ttataaagca gcaaatgcta aagaaaatta
     1381 ttggtttggt actgatcagt tgggtcgaga tttatggaca agaacatgga aaggtgctca
     1441 aatttcattg tttatcggtg ttgttgcagc gatgttagat atttttattg gcgttgtata
     1501 tggtgcgatt tctggattct tcggtggacg tgtcgatacg attatgcaac gtatacttga
     1561 agtcatagca tctattccga atttaattgt cgtaatttta tttgtattaa tttttgaacc
     1621 atccatttgg acaattatat tggctatgtc tatcacaggc tggttaggca tgagcagagt
     1681 tgtacgtgga gaatttttaa aattaaaaaa tcaagagttt gtcatggctt cgaaaacatt
     1741 gggggcttca aaattcaaat tgatatttaa gcatatttta cctaatacat taggtgctat
     1801 cgtggttaca tcaatgttta cagtacctag tgctattttc ttcgaagcat ttttaagttt
     1861 cattggtata ggtgtacccg cacctcaaac atcgttaggg tcattagtaa atgatgggcg
     1921 cgcaatgtta ttaatttatc cacatgaatt atttatacca gcaatgattt taagtttatt
     1981 aattctattc ttttacttat ttagtgatgg attacgtgat gcatttgatc cgaaaatgcg
     2041 taaataaaag ggggcatagc atatgactga aagaatatta gaagtaaatg atttgcatgt
     2101 ttcctttgat attacagcag gggaagtgca ggcagtgaga ggtgtagatt tttatttaaa
     2161 caaaggggaa acattggcaa ttgttggtga atcaggttca ggtaaatctg taacaacaaa
     2221 agcaattaca aaattattcc aaggggacac aggaagaatt aaaaagggag aaattttatt
     2281 tttagaggaa gatttagcaa aaaaacctga aaatgagtta attaaattac gtggcaaaga
     2341 tatttcaatg atctttcaag atccaatgac atctttaaac ccaacgatgc aaattggtaa
     2401 acaagtcatg gaaccattaa ttaagcacaa aaattatagt aaagcacaag ctaaaaagcg
     2461 cgcattggaa atactaaatc ttgtaggttt accaaatgca gaaaaaagat ttaaagcata
     2521 tccacatcaa ttttcaggtg gacaaaggca aagaattgtt attgcaaccg cattagcttg
     2581 tgaacctaaa gtgctcattg ctgatgaacc aacgactgca ttagacgtaa cgatgcaggc
     2641 acaaatttta gatttaatga aagaactaca acaaaaaatc gatacagcaa ttatttttat
     2701 aacgcatgat ttaggggttg ttgcgaatat tgctgataga gtggcagtta tgtatggtgg
     2761 tcaaatggtt gaaacaggag atgttaacga aatattttat gatccaaagc atccatatac
     2821 atggggatta ttatcgtcaa tgcctgattt atcaacaaca aatgacacac cattactagc
     2881 gattcctgga gcgccacctg atttattaca cccacctaaa ggtgatgcat ttgcgagacg
     2941 tagtcaatat gcattagata ttgattttaa agtagaacca ccgtggttta aagtttcacc
     3001 gacacatttt gtgaaatctt ggttattaga cgcacgtgca ccaaaagttg aactacccga
     3061 gctggtaaaa caatgtatga aaccgatgcc taataattat gaaaaaccac tcaaggtaga
     3121 aagggtgtcg ttcaatgaaa aatgatgaag tgctattatc tattaaaaat ttaaagcaat
     3181 attttaacgc aggaaagaaa aacgaagtga gagcgattga aaatatttcg tttgaattat
     3241 acaaagggga aacattaggt ttagtaggag aatcggggtg tggtaaatct acaactggta
     3301 aatcaattat caaacttaat gatattacaa gtggagaaat tttgtatgag ggtattgata
     3361 tacaaaagat tcgtaaacgc aaagatttgc ttaaatttaa taaaaagata cagatgattt
     3421 ttcaagaccc atatgagtct ttaaatccta ggttaaaagt aatggatata gtagctgaag
     3481 gtattgatat ccatcattta gcaactgata aacgtgaccg taaaaaacgt gtctatgatt
     3541 tacttgaaac tgttggatta agtaaagaac atgccaatcg ttatcctcat gaattttcag
     3601 gcggacaacg ccaacgtatt ggaattgccc gtgcattagc cgttgaacca gaatttatta
     3661 ttgcagatga acctatttca gcattggatg tttcgattca agctcaagta gtcaatttgt
     3721 tattaaaatt acagcgtgaa aaaggtatta cattcctatt tattgcacat gatttatcaa
     3781 tggtgaagta tatctctgat cgaattgcag tcatgcattt tgggaaaatt gtagaaatcg
     3841 gaccagcaga cgatatttat caatatccat tacatgatta tactaagtct ttattgtcgg
     3901 caattcctca accggatcct gaatcggaac gtagtcgcaa acgatttagt tatattgatg
     3961 atgaagcaaa taatcattta agacaattac atgaaataag accacagcat tttgtcttta
     4021 gtactgaaga agaagcggca caactacgag aaaataaatt ggtgacacaa aattaaggga
     4081 agggggaaat gcaatgacga gaaaatttag aacacttatt ttaattttga ttgctacaat
     4141 tgcattaagt ggttgtgcta atgatgatgg tatttattca gataaaggtc aagtattcag
     4201 aaaaattttg tcatcagact taacatccct tgatacatca ttaataacgg atgaaatatc
     4261 ttctgaagtg actgcgcaaa cattcgaagg tttatacaca ttaggaaaag gtgacaaacc
     4321 ggtgttaggt gttgcgaaag cttttcctga aaagagtaaa gatggtaaaa ctataaaggt
     4381 taaattaaga agcgatgcta aatggagcaa tggtgacaaa gtgactgcac aagactttgt
     4441 ttatgcttgg agaaaaacag ttgaccctaa aacaggttct gaatttgcat acattatggg
     4501 ggacattaaa aatgcgagtg atattagtac tggtaagaaa cctgtagagc aattaggtat
     4561 caaagcatta aatgatgaaa cattacaaat tgaattagaa aagccggttc catatattaa
     4621 tcaattatta gcacttaata catttgcacc tcaaaatgaa aaagttgcca aaaaatatgg
     4681 taaaaattac ggtacggcag ctgatagagc ggtatacaat ggtccattta aagttgatga
     4741 ttggaaacaa gaagataaaa ccttactatc taaaaatcag tattattggg ataaaaagaa
     4801 tgtaaaatta gataaagtga attataaagt tattaaagac ttacaagccg gtgcatcatt
     4861 gtatgatact gaatcagtag atgacgcagt tattactgca gatcaagtaa ataaatataa
     4921 agataacaaa ggattaaact ttgtgttaac gactgggaca ttttttgtaa aaatgaatga
     4981 aaaacaatat cctgatttta aaaacaaaaa tttaagactg gctatcgcac aagcaataga
     5041 taaaaaagga tacgttgatt cagtgaaaaa caatggctca attccttccg atacactaac
     5101 agccaaagga attgcgaaag caccgaatgg caaagattat gcgagtacca tgaattcacc
     5161 gttaaaatat aatcctaaag aagcaagagc acattgggaa aaagctaaaa aagagttagg
     5221 taaaaatgaa gtgacatttt ctatgaacac agaagatacg ccagatgcaa aaatatctgc
     5281 agaatatatc aaatcgcaag ttgagaaaaa tttaccaggt gttactttaa aaattaagca
     5341 attgccattt aagcaaagag tttcattaga gttaagtaac aattttgaag catcacttag
     5401 tggttggtct gcagattacc ctgatcctat ggcttattta gaaacaatga ccacaggtag
     5461 cgcacaaaat aatacagact ggggtaataa agaatatgat caattactta aagtagcaag
     5521 aaccaaattg gcacttcaac cgaacgaacg atatgaaaac ttgaaaaaag cagaagaaat
     5581 gttcctagga gatgcaccgg tagcaccaat ttatcaaaaa ggtgttgcac atttaacaaa
     5641 tcctcaagta aaaggattaa tttaccataa atttggtcca aataactcac ttaaacatgt
     5701 ctatattgat aaatcgatag ataaagaaac aggtaagaag aaaaaataat atgctttgta
     5761 aattaggctg gagacatatc tccagtcttt ttgtgttgga tagaaacttt gggaataaaa
     5821 atttaaaata agtcgttttt taaattactg aaattgatta aatgcataaa taactgaata
     5881 ttctaaaaat aaacttgtaa taattttttc tatgagtaaa ctaaaaagaa aaattagatt
     5941 gaaagtagga ggcatataaa tggggaagct aattaaatat atttcaatac ttattgttat
     6001 cgttttagtg ttgagtgctt gcggaaaaag cagtaataaa gacgaaggag taaaagatgc
     6061 tactaaaaca gaaacctcaa aacataaagg tggtacctta aatgtagcat taacagcacc
     6121 tccaagtggt gtttattctt cgttattaaa tagtacacat gcggatgctg tagttgaggg
     6181 atattttaac gaaagcttat tagcaactga taaaaaaata cgtcctaagg catatattgc
     6241 ttcatggaag gacatcgagc cggctaagaa aatagaattt aaaattaaaa aaggtattaa
     6301 atggcatgat ggtaatgaat tgaaaattga tgattggatt tattcaattg aagtcttagc
     6361 taacaaggac tacgaaggtg cttattatcc aagtgtagaa aatatccaag gtgcgaaaga
     6421 ttatcatgaa ggaaaaactg atcatattag cggattgaag aaaatagatg actacactat
     6481 gcaggttaca tttgataaaa aacaagaaaa ttacttaaca ggatttatta ctggaccttt
     6541 attaagtaaa aaatatttat cagatgtacc aattaaagat ttagcgaaat cagataaaat
     6601 ccgaaaatat cctattggta ttggaccgta taaagttaag aaaatcgttc caggtgaggc
     6661 tgttcaactc gttaaatttg atgattattg gcaaggtaag cctgcactag acaaaatcaa
     6721 tttaaaagtt attgatcaag ctcaaattat taaggcaatg gaaaaaggcg atattgatgt
     6781 tgcgaatgat gctaccggtg caatggcaaa agatgctaag tcatctaatg ctggtctcaa
     6841 ggtattatct gcgccaagct tagactacgg tttaatagga ttcgtatctc atgattacga
     6901 taaaaaatct aataaaactg gtaaagtgag accaaaatat gaagacaaag aattacgtaa
     6961 agcaatgctt tatgcaattg atagagaaaa atggatcaaa gcatttttca atggttacgc
     7021 tagtgaaata aatagttttg taccatcaat gcattggata gctgctgatc ctaaggaact
     7081 aaacgattat aaatatgatc ctgaaaaagc taaaaaaatc ttagataagt taggttataa
     7141 agatagagat ggtgatggat ttagagaaga tcctaaaggt aataaatttg agattaactt
     7201 taaacattat tcaggttcaa atcctacttt tgaaccaaga actgctgcga taaaagattt
     7261 ctgggaaaaa gttggcttga aaacaaatgt gaagttagta gaattcggta aatataatga
     7321 agacttagca aatgcatcta aagatatgga agtgtacttt agatcatggg caggaggtac
     7381 agatccagat ccatcagatt tataccacac tgatagacct caaaatgaaa tgagaacagt
     7441 tttaccaaaa tcagatcaat atttagatga tgcattagac ttcgaaaaag taggcattga
     7501 tgaaaagaaa cgtaaagata tttatgttaa atggcaaaaa tatatgaatg atgagttacc
     7561 tggattacca atgttccaag gtaaatcgat aactattgtt aacgataaag tacgaaactt
     7621 agacattgaa attggaactg atcaaagttt atataattta actaaagaag cttagtaatg
     7681 taacctcgac gattttgtcg aggtttactt atatgggggg atgttatgaa taatgtattg
     7741 ttagaggtta aagatttaga aacatcatta aaaataaata atgaatggtt agcaactgtt
     7801 gaaaatattt cttttgaatt atctaaagga gaagttttgg gtatagtagg ggaatctggt
     7861 tgcggtaagt ccatattaag taagtcaatt attaaattat taccagaaaa gatatctaaa
     7921 ctaagtagtg gagaagttat atttgatggt aaacgaatcg atacgctcaa tgagaagcaa
     7981 ttgctagata ttcgaggaaa tgatattgct atgatttttc aagaacctat gactgcttta
     8041 aatcctgtat ttaccataaa aaatcaactt gtggaatcta taaaatcaca taaaaaaatt
     8101 tctaaaaaag aagcaaataa cttagcaaaa gatttactaa aaaaagttgg aattgctaga
     8161 caagatgaaa tattaaatag ctatcctcat caattatctg gtggtatgag acaaagagta
     8221 atgattgcaa tggccatttc atgttctcct aaattattaa ttgctgatga acctacaaca
     8281 gcattggatg tcacgattca agcgcaaata ttagacttat taaaagaatt gcaaaaggaa
     8341 acgcaaatgg caattatgat gattacacat gatttgagtg tagttgctga gttttgcgat
     8401 aaagtcttag ttatgtatgc aggtcaaatt gtagaatttg gaggcataaa agaaatacta
     8461 cacaatccga aacatcctta tacccaaaaa ttattatcaa caattccaaa acttaaagaa
     8521 gagcagaaac gacttgaaac gatagaagga attgtgccat caatccaagc atttcacgtt
     8581 aataagtgca gatttgcaaa tagatgtaac aaaaaaatgg atatttgtaa taatcaatct
     8641 cctaaaatgc atgtttgtga agacgtcatt gtacgttgtc atttgtacaa aaatgaatat
     8701 aaggagatat aagaatggaa aatattttag aagtcaacca aataaaaaaa tactacaaaa
     8761 ttaaaactgg attattacaa aaaactcagt acgttaaagc tgttgatgac gtatcgtttt
     8821 caataaaaaa aggacaaact tttggattag taggagaatc gggttgtggt aagtcaacgt
     8881 taggtaaagt gattatcagg cttgaagatg caacttcagg ctcaataatt gttaatggtg
     8941 aagatataac aagattacaa ggtaaaaaac tcagaaaatc acgacaacaa tatcagatga
     9001 tatttcaaga tccgtatgca tcattgaatc cgatgcaaat ggttggagat atcatttcag
     9061 aacctatttt aaattataaa aaattgccaa aagaagaaat aaaaaaagaa gtactatatt
     9121 tattaaaatg tgttggccta agtgaagatg catattataa atatgcacat gaattttcag
     9181 gtggacagag acaaagagtg ggaattgcaa gagcattggc tttgcgtccg agtttaattg
     9241 ttgctgatga gcctgtaagt gcattagatg tatctgttca atctcaagta ctgaatttat
     9301 taaaagattt acaagaacaa tttaacttaa gctatttatt tatcgcacat gatttaagtg
     9361 tagtaaaaca tataagtgat gtcattggag ttatgtattt aggtcatata gttgaaatcg
     9421 catctgataa agaaatttat gaaaatccca aacatccata tacaaaagcg ttgatttcat
     9481 caataccaca aattgataaa cataataaca atagaattat attaaaagga gaattacctt
     9541 cgccaagtaa tccgccgcaa ggttgtcctt ttcatacaag atgtccgatt gcaaaagata
     9601 aatgtaaaga aaatatacca caattaaagg acattggtga tgaacatcaa gttgcttgtt
     9661 tttatgtaaa taaagtaggt gatttaaatg gttaaattaa tattaaagag attaggttta
     9721 atgattccgt tactaatttt aatttctatt gttgtatttt cattagctat cattcaacca
     9781 ggagatccat tttcagattt acaaaacgga aaaataaaac aagaagcgat aaatgcacaa
     9841 agggaaaagt taggcctcaa cgactctata tcacatcaat acattagatg ggtcaatcat
     9901 gttatacatg gtgatttagg ggagtcaatc aaatataaaa ggccggtaat tgatgttatt
     9961 gaggaaagaa ttccaaatac aatattactc ggtgctatgt cattaattat tacttatatt
    10021 atctcatttg ctttaggaat aacgtcaggt agatattctt acagtttgac tgattatact
    10081 gtgcaaatat ttaattattt gatgttagct attccatctt ttattgcggg agtatttgca
    10141 atttttattt tttcttttga attacaatgg tttccgtttc aaggttctgt tgatattaac
    10201 cttaaagaag gtacttttga atattatatg agtaaaattt atcatacatt tttgcctgca
    10261 ttaactttag gattattatc tactgctggt tatattcaat atttacgtaa tgatattatt
    10321 gaaaattcta aaaaatatta tgtattgacg gcaaggtcaa aaggattatc tatgaataaa
    10381 atttataata aacatatatt gagaaattct ttaataccta ttattacatt tttaggtgct
    10441 gatattgtaa gtattttagg tggagctgtg attactgaga ctatcttttc atataacggt
    10501 atcggtaaat tatttttaga atcggtaata ggtcaagact atccattaat gatggcatta
    10561 acgttgtttt tctcattttt aggtttactg ggtaatttga tttctgatat tacttatgga
    10621 tttatagatc caagaattag aagtaactag gggggattgg aatgcaaaat aagtcaaaat
    10681 cgccttttaa aattgcattg tctaaattta ctcataataa aattgcaatg ttatcgatta
    10741 tttttttatt aatcataact attgtatcaa ttatagcgcc attaatagct ccttttccag
    10801 tgaaccaaca agatttatta aatataaaag gtgaaatgac agcacaaaac attcttggta
    10861 cagactccgg tggtagggat aactttagtc gtttgttata tgcaggtcgt atttcattat
    10921 ccattggaat tacatctaca ataggaatgc ttttgattgg aattacagtt ggagtgattt
    10981 ctggttattt tggaagtatt gttgatacat tattaatgag aataaccgaa tttgttatgt
    11041 tatttccatt tttaatattt gcaattgtat taaatgctgc acttggagat aaaattaaaa
    11101 atccttatgg atctgccata attcttgttc tagttattat cgtattaagt tggggaggta
    11161 ttgcaagact tgttcgtggt aaagtacttc aagaaaaaga aaatgaatac tttttggcag
    11221 caaaatcaat tggtacaccc acatataaaa ttattttgaa acatcttttg ccgaatatat
    11281 taagtgtagt tatcgtacaa gcaacattgt tatttgccgg tatgattgta gtggaatcag
    11341 gattgagttt tttaggattc ggaattagta aagcaatccc atcttggggt aatatgttga
    11401 gtgatgctca agaaggggat gttataagtg gtaaaccgtg gatatggatg ccacctgcta
    11461 taatgattac attaactata ttaagtataa actttgtagg tgaagggctt aaagatgctt
    11521 ttaatcctag aggtagacgt taaataataa acaggctggg acat
//
LOCUS       DAHHOJ010000082        11473 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.082, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000082 DAHHOJ010000000
VERSION     DAHHOJ010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11473
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.082"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..686)
                     /gene="seo"
                     /locus_tag="NBH32_001912"
     CDS             complement(<1..686)
                     /gene="seo"
                     /locus_tag="NBH32_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010922839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type O"
                     /protein_id="HCD1515504.1"
                     /translation="MKNSKVMLNVLLLILNLIAICSVNNAYANEEDPKIESLCKKSSV
                     DPIALHNINDDYINNRFTTVKSIVSTTEKFLDFDLLFKSINWLDGISAEFKDLKVEFS
                     SSAISKEFLGKTVDIYGVYYKAHCHGEHQVDTACTYGGVTPHENNKLSEPKNIGVAVY
                     KDNVNVNTFIVTTDKKKVTAQELDIKVRTKLNNAYKLYDRMTSDVQKGYIKFHSHSEH
                     KESFYYDLFY"
     gene            complement(899..987)
                     /locus_tag="NBH32_001913"
     tRNA            complement(899..987)
                     /locus_tag="NBH32_001913"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(950..952),aa:Ser,seq:gga)
     gene            complement(1041..1112)
                     /locus_tag="NBH32_001914"
     tRNA            complement(1041..1112)
                     /locus_tag="NBH32_001914"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1077..1079),aa:Glu,seq:ttc)
     gene            complement(1114..1188)
                     /locus_tag="NBH32_001915"
     tRNA            complement(1114..1188)
                     /locus_tag="NBH32_001915"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1154..1156),aa:Asn,seq:gtt)
     gene            complement(1203..1276)
                     /locus_tag="NBH32_001916"
     tRNA            complement(1203..1276)
                     /locus_tag="NBH32_001916"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1242..1244),aa:Gly,seq:tcc)
     gene            complement(1292..1367)
                     /locus_tag="NBH32_001917"
     tRNA            complement(1292..1367)
                     /locus_tag="NBH32_001917"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1332..1334),aa:His,seq:gtg)
     gene            complement(1381..1453)
                     /locus_tag="NBH32_001918"
     tRNA            complement(1381..1453)
                     /locus_tag="NBH32_001918"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1418..1420),aa:Phe,seq:gaa)
     gene            complement(1470..1545)
                     /locus_tag="NBH32_001919"
     tRNA            complement(1470..1545)
                     /locus_tag="NBH32_001919"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1510..1512),aa:Asp,seq:gtc)
     gene            complement(1567..1640)
                     /locus_tag="NBH32_001920"
     tRNA            complement(1567..1640)
                     /locus_tag="NBH32_001920"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1604..1606),aa:Met,seq:cat)
     gene            complement(2033..2587)
                     /locus_tag="NBH32_001921"
     CDS             complement(2033..2587)
                     /locus_tag="NBH32_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase family protein"
                     /protein_id="HCD1515505.1"
                     /translation="MTDVIIVHSMHGNSRNHWYQWLEHNLTLEGYDVTLFNFESPEAK
                     TVDQWIEAMTKQINVRKKDTYFVTHGLGSITALKYIEMIDQPIEGFFSIAGFKEDAEN
                     IDLDIDLSNVTIDYDNIKKKVDNFYGLSSKNDKYVSYKETQRLMNALEGNLRIVEDGG
                     HFLEEDGFETFTALQDRMQDYMTR"
     gene            complement(2910..4310)
                     /locus_tag="NBH32_001922"
     CDS             complement(2910..4310)
                     /locus_tag="NBH32_001922"
                     /EC_number="1.3.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoporphyrinogen oxidase"
                     /protein_id="HCD1515506.1"
                     /translation="MTKSVAIIGAGITGLSSAYFLKQQDPNIDVTIFEASNRPGGKIQ
                     SYRKDGYMIELGPESYLGRKTIMTELAKDIGLEQDIVTNTTGQSYIFAKNKLYPIPGG
                     SIMGIPTDIKPFVTTKLISPLGKLRAGLDLLKKPTQMQNGDISVGAFFRARLGNEVLE
                     NLIEPLMGGIYGTDIDKLSLMSTFPNFKEKEEAFGSLIKGMKDEKNKRLKQRQLYPGA
                     PKGQFKQFKHGLSSFIEALEQDVKNKGVTIRYNTSVDDIITSQKQYKIVYSNQQEEVF
                     DGILVTTPHQVFLNWFGQDPAFDYFKTMDSTTVATVVLAFDEKDIENTYDGTGFVIAR
                     TSDTDITACTWTSKKWPFTTPEGKVLIRAYVGKPGDTVVDDHTDNELVSIVRRDLSQM
                     MTFKGDPEFTIVNRLPKSMPQYHVGHIQQIRQIQAHIKQTYPRLRVTGASFEAVGLPD
                     CITQGKVAAEEVIAEL"
     gene            complement(4334..5257)
                     /gene="hemH"
                     /locus_tag="NBH32_001923"
     CDS             complement(4334..5257)
                     /gene="hemH"
                     /locus_tag="NBH32_001923"
                     /EC_number="4.99.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCD1515507.1"
                     /translation="MTKKMGLLVMAYGTPYKESDIEPYYTDIRHGKRPSEEELQDLKD
                     RYEFIGGLSPLAGTTDDQADALVSALNKAYADVEFKLYLGLKHISPFIEDAVEQMHND
                     GITEAITVVLAPHYSSFSVGSYDKRADEEAAKYGIQLTHVKHYYEQPKFIEYWTNKVN
                     ETLAQIPEEEHKDTVLVVSAHSLPKGLIEKNNDPYPQELEHTALLIKEQSNIEHIAIG
                     WQSEGNTGTPWLGPDVQDLTRDLYEKHQYKNFIYTSVGFVCEHLEVLYDNDYECKVVC
                     DDIGANYYRPKMPNTHPLFIGAIVDEIKSIF"
     gene            complement(5315..6352)
                     /gene="hemE"
                     /locus_tag="NBH32_001924"
     CDS             complement(5315..6352)
                     /gene="hemE"
                     /locus_tag="NBH32_001924"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCD1515508.1"
                     /translation="MVHNKNNTILKMIKGEETTHTPVWFMRQAGRSQPEYRKLKEKYS
                     LFDITHQPELCAYVTHLPVDNYHTDAAILYKDIMTPLKPIGVDVEIKSGIGPVIHNPI
                     KTIQDVEKLSQIDPERDVPYVLDTIKLLTEEKLNVPLIGFTGAPFTLASYMIEGGPSK
                     NYNFTKAMMYRDEATWFALMNHLVDVSVKYVTAQVEAGAELIQIFDSWVGALNVEDYR
                     RYIKPHMIRLISEVKEKHDVPVILFGVGASHLINEWNDLPIDVLGLDWRTSINQAQQL
                     GVTKTLQGNLDPSILLAPWNVIEERLKSILDQGMENGKHIFNLGHGVFPEVQPETLRK
                     VSEFVHTYTQR"
     gene            complement(6342..6449)
                     /locus_tag="NBH32_001925"
                     /pseudo
     CDS             complement(6342..6449)
                     /locus_tag="NBH32_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500462.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            6614..7117
                     /gene="traP"
                     /locus_tag="NBH32_001926"
     CDS             6614..7117
                     /gene="traP"
                     /locus_tag="NBH32_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein TRAP"
                     /protein_id="HCD1515509.1"
                     /translation="MKKLYTSYGTYGFLNQIKINNPSHHLFQFSTTDSSVIFEETEEN
                     TVLKSPSIYEVIKEIGAFNEDHFYCAIFIPSTEDHVYQLEKKLISVDDNFKNFGGFKS
                     YRLLRPVKGTTYKIYFGFADRQTYEDFKNSDAFKDHFSKEALSHYFGSSGQHSSYFER
                     YLYPIKE"
     gene            complement(7241..8464)
                     /locus_tag="NBH32_001927"
     CDS             complement(7241..8464)
                     /locus_tag="NBH32_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515510.1"
                     /translation="MRNQATTLFNKRLHALRKEKNYYNKFIFNGHFMVFLLILLGAFI
                     FGYGEWLKHIPTNINFALIAAVIVALTSIFPMRPLLKEADKIFLLPFEKHMSQFMRHA
                     ILYSYFARILIQLIIVIVMFPLFYNINQHNVAFYICFGVSALIFPYVGLRIRWQWYQS
                     GLKTWQVNLISFIIFALTYYLLLAPKWYIAFVMVALPVLIEFLVKKYKPGFLYPWEKM
                     IAIEHRHHMNYYKFVNMFTDVKHLKESAVRRSYLDILLPVPKGSKFNSNAMYLFLFIR
                     SFIRGRDAFNIIFRLVIIAVLLMLWLSYPLVTAIIGCLFVYIILLQMAQFYSQQAYGL
                     WPQVWPVPEEKVIKGYEQFLYRLMFVICTVFAVAFIIKHMTLFYVVLIFYIVGLLTIR
                     SIIKKLKYQETLLRD"
     gene            complement(8457..9197)
                     /locus_tag="NBH32_001928"
     CDS             complement(8457..9197)
                     /locus_tag="NBH32_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515511.1"
                     /translation="MTVKVEQLTGGYGKRPVIKDINFELNKGEIVGLIGLNGAGKSTT
                     IKHMLGLLTPMEGSLSISDININDDIEAYRRKLSYIPESPVIYEELTLEEHIEMTAMA
                     YDIDRDEAMNRAMPLLKTFRLENELKVFPSHFSKGMKQKVMIICAFIVNPELYIIDEP
                     FLGLDPLGIQSMLDLMVEKKNEGRTVLMSTHILATAERYCDRFIILDEGEVVAFGDLE
                     ALRQQTGLHNQTLDDIYIHVTQGGDVHA"
     gene            9331..9753
                     /locus_tag="NBH32_001929"
     CDS             9331..9753
                     /locus_tag="NBH32_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HCD1515512.1"
                     /translation="MSETIFGKILTGEIPSFKVYEDDYVYAFLDISQVTKGHTLLIPK
                     KASANIFETDEETMKHIGAALPKVANAIKRAFNPDGLNIIQNNGEFADQSVFHIHFHL
                     IPRYENDIDGFGYKWETHEDILDNDAKQQIAEQIQAQF"
     gene            9895..10260
                     /locus_tag="NBH32_001930"
     CDS             9895..10260
                     /locus_tag="NBH32_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HCD1515513.1"
                     /translation="MKASRILFGIGVGVAAGFVVALQGRDDKSVKNNTIDRTAPTGSK
                     SELQREFETIKQSFNDILNYGVQIKNESAEFGSSIGGEIKSLLGNFKSDINPNIERLQ
                     SHIENLQNRGEDIGNEISK"
     gene            10993..>11473
                     /locus_tag="NBH32_001931"
     CDS             10993..>11473
                     /locus_tag="NBH32_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HCD1515514.1"
                     /translation="MFLCKRQIDINARFGLPRIAFMSAVATIIMFLVSYEVMYFLSNT
                     PLSDRHFLIFLLLVFMTYPLHKSIHLLFFLPYRKSFKVHKLTKRKWLIFYNTYVNQPV
                     HKFYFCINLILPLIILSAMFVYLTISFPQYGHYFMFLLALNFGISITDLLYLKIIIFS
                     "
BASE COUNT         3747 a         2112 c         1623 g         3991 t
ORIGIN      
        1 atataaaata aatcataata aaatgattct ttatgctccg aatgagaatg aaatttaata
       61 taaccttttt gtacatcact agtcattctg tcatacaatt tatatgcatt atttaatttt
      121 gttcttactt taatatcaag ttcttgtgca gtaactttct ttttatctgt agtaacgata
      181 aatgtattaa catttacatt atccttatac acagctactc ctatattttt aggctcgctt
      241 aatttattat tttcatgagg tgttacccca ccatatgtac aggcagtatc cacttgatgc
      301 tcaccatgac aatgtgcttt atagtaaaca ccataaatat caacagtctt tcctagaaat
      361 tctttagaaa tcgctgatga gctaaattcc acttttaaat ctttaaattc agcagatatt
      421 ccatctaacc aattaatact tttaaataat aaatcgaagt ctaagaattt ttctgtagtt
      481 gatacaattg attttactgt cgtaaatcga ttatttatat aatcatcatt aatattatgt
      541 aaagcaatag ggtctacact tgacttctta cacaaactct ctattttagg atcttcttca
      601 tttgcatatg cattgtttac actacatatt gcaattaaat ttaaaattaa taataataca
      661 tttaacatta ctttactatt tttaataata tccctccttc acaaattttt ttcttaataa
      721 actgaaaaat tctgttttta atctttaaat attcactaat attattagat tacgatttta
      781 aacttaacaa tcctacttac tattgaacaa acaaatttta aattaagtga ctcagataat
      841 agacaaaaaa ccttaaaaat agcgcttaaa atgggtaaaa ccattttaag cgcctttacg
      901 gagagtgagg gattcgaacc ctcgagacgc ttgtggcgcc tacacacttt ccaggcgtgc
      961 tccttcggcc aactcggaca actctcctca acaataaata tgtaataaaa aacagaagcg
     1021 atatttcact tctgtttgta tgactcctac gggactcgaa cccgtgttac cgccgtgaaa
     1081 gggcggtgtc ttaaccgctt gaccaaggag ccatggctcc acaggtagga ctcgaaccta
     1141 cgaccgatcg gttaacagcc gatagctcta ccactgagct actgtggaat aatagaaaat
     1201 aatggagcgg gtgatgggaa tcgaacccac aacatcagct tggaaggctg aggttttgcc
     1261 attaaactac acccgctaat gtaaacgatt atggggcggc tgaaggggat cgaaccctcg
     1321 aatgtcggaa ccacaatccg atgtgttaac cacttcacca caaccgccat gaattaagaa
     1381 tggttcagga cagagtcgaa ctgccgacac atggagcttc aatccattgc tctaccaact
     1441 gagctactga accgtaatgg taataataat ggcggtctcg acgggaatcg aacccgcgat
     1501 ctcctgcgtg acaggcaggc gtgttaaccg ctacactacg agacctatta aattaaaaac
     1561 tatgtattgc gggaggcgga tttgaaccac cgaccttcgg gttatgagcc cgacgagcta
     1621 ccgaactgct ccatcccgcg ctaatattat tttgaattac ctaattaata taccataatc
     1681 aaaaacctaa agtcaagaac tttttgaatt taatttaaat gttatctctt aaataattac
     1741 ttaaatatcg tagcaacatg ttctctgttg aacacaaata ttagtatatt catttttgta
     1801 gtgtacgtca acgacatttt caaagttttt tgtgtaaaaa aacgcttctt cttccctttt
     1861 ataatataag tgtctaatag ttgtcataat tactcatgaa ctattgaaaa agatgtagga
     1921 atcataaatt ttataatgcc tacacctcta aactattaaa atataattat aatctttttc
     1981 actaaagtac tatgcttcgt actgacacaa cataacacaa cccatacact aactatcgtg
     2041 tcatgtaatc ttgcatccga tcttgcaacg ctgtaaatgt ttcgaagcca tcctcttcta
     2101 agaagtgccc tccatcttcc acgattcgca agttcccctc taatgcattc attaaacgct
     2161 gggtttcttt atatgaaacg tatttgtcat ttttagaact caatccgtaa aaattgtcaa
     2221 ctttcttttt aatattatcg taatcaatgg ttacattact taaatcaata tctaaatcta
     2281 tattttctgc atcttcttta aagcccgcta tactaaaaaa gccttcaatc ggctgatcaa
     2341 tcatttcaat atattttaaa gctgtgattg aacctaaacc atgtgttaca aaatatgtat
     2401 cctttttgcg tacattaatt tgtttcgtca tagcttcaat ccactgatcc actgtcttcg
     2461 cttcagggga ttcaaaatta aataatgtta cgtcatatcc ttctaaagtt aagttatgct
     2521 ccaaccactg ataccaatga tttctactat ttccatgcat agaatgtaca ataattacat
     2581 ctgtcatctc attctctcct ttcaacttac tacttctttt ctatttttaa aaaaatgact
     2641 gattacctat aattgtaaaa taaaaacacc ttaattagaa atgttatatc gcaaagtgac
     2701 atttctaatt aaagtgtatt gacatcattt caatatcatt aaaaaacagc taaacctttg
     2761 tctctgcttc aatttcacaa aattaattcc cgctgaaagt atctatattt acacattact
     2821 tccaccatta tataacttaa aaatgactat atttcatcaa acattatcta aaggcgtcgc
     2881 acctacacca acaccatcca acaattaact tacaactctg cgattacttc ttcagcagca
     2941 actttacctt gcgtaataca atcaggtagt ccaaccgctt caaaagatgc accagttact
     3001 ctaagtcgtg gatatgtttg tttaatatgt gcttgaatct gtctaatttg ttgaatatga
     3061 ccgacatggt actgtggcat acttttcggc aaacgattga caattgtaaa ttcaggatca
     3121 cctttaaatg tcatcatttg acttaaatct ctacgtacaa tcgatactaa ttcattatct
     3181 gtatgatcat caaccacagt atcacctggt ttacctacat acgcacgaat caaaacctta
     3241 ccttctggtg tagtaaatgg ccattttttc gatgtccaag tacatgcggt aatgtctgta
     3301 tcactcgttc tcgcaatcac aaagccagta ccatcataag tattttcaat gtctttttca
     3361 tcaaatgcca atacaacagt tgcaacagtc gtactatcca tcgttttaaa gtaatcaaat
     3421 gctggatctt gcccgaacca attcaaaaag acttgatgtg gtgttgtcac taatattcca
     3481 tcgaatactt cttcttgttg attactgtaa acaattttat attgcttttg agatgtaata
     3541 atatcatcca ctgacgtatt gtagcgtatt gtcacacctt tatttttcac atcttgttct
     3601 aatgcttcaa taaatgagct taaaccatgc ttaaattgtt tgaattgtcc ttttggtgcg
     3661 ccaggatata attgtctttg tttcagacgc ttatttttct catccttcat accttttatc
     3721 agacttccga atgcctcttc tttttcttta aaattaggaa acgtactcat caaacttaat
     3781 ttatcaatat cggtaccata aataccaccc attaaaggct caattaaatt ctcaagtacc
     3841 tcattaccta atcttgctct gaaaaatgca ccaacagaaa tgtcaccatt ctgcatttga
     3901 gtaggttttt taagtaaatc taaccctgct cttaactttc caagtggtga aattaattta
     3961 gttgtcacaa acggtttgat atctgtcgga attcccataa ttgatccacc tggaatagga
     4021 tacaatttgt ttttcgcaaa aatataagat tgtccagtcg tatttgtaac aatatcttgt
     4081 tctaatccaa tatctttcgc taattctgtc ataatcgttt ttctacctaa ataagattca
     4141 ggccctagtt caatcatata accatcttta cgatacgatt gaatctttcc ccccggacga
     4201 ttcgatgctt caaagatggt tacatcaata ttaggatctt gctgttttaa aaaatatgca
     4261 cttgataaac ctgttatccc cgctcctata atagccactg atttagtcac aacgtttcac
     4321 gcttctttcg tcattaaaat atagacttga tttcatcaac aattgcaccg ataaataatg
     4381 gatgtgtatt cggcattttt ggacgataat aattcgcacc aatatcatcg caaacaactt
     4441 tacattcata atcattgtca taaagcacct ctaaatgctc acatacaaaa cctactgacg
     4501 tatatataaa gtttttatac tgatgttttt catataaatc acgtgttaaa tcttgtacat
     4561 ctggccctaa ccaaggtgta cctgtattac cttcagattg ccaaccaatc gcgatatgtt
     4621 caatattaga ttgttcttta attaaaagtg cagtatgttc tagttcttgt ggatatggat
     4681 cattattctt ttcgattaaa ccttttggca aactatgagc cgaaacaact aataccgtgt
     4741 ctttatgttc ctcttccggt atttgagcta atgtttcgtt gactttattc gtccaatatt
     4801 caataaattt aggttgttca taataatgtt tcacatgtgt aagttgaata ccatattttg
     4861 cagcttcttc atcagcacgt ttgtcatatg atcctactga aaatgaagaa tagtgtggtg
     4921 ctagtactac agtgattgct tcagtaatgc catcattgtg catttgttca accgcatctt
     4981 cgataaatgg cgaaatatgt tttaatccta aatatagttt aaattcaaca tctgcatatg
     5041 ctttatttaa tgctgaaact agcgcatcag cttggtcatc tgttgtacct gctaatggtg
     5101 ataaaccacc tataaattca tatctatctt tcaaatcttg aagttcttct tcagatggac
     5161 gtttaccatg tctaatatct gtataatatg gctctatgtc actttcttta taaggtgtgc
     5221 cataagccat aactaataat cccatttttt tagtcattga taataccttc ctttaaatga
     5281 attatctttc atgtgcttca atgtaatact atgattatct ttgtgtatat gtgtgtacga
     5341 attcgcttac tttacgtaac gtctctggtt gcacttctgg gaaaacaccg tgtcctaaat
     5401 taaagatgtg tttaccgttc tccatacctt gatctaatat tgacttcaat ctctcttcaa
     5461 tgacattcca tggtgctaat aaaattgatg gatctaaatt cccttgtaat gttttagtaa
     5521 cacctaattg ctgtgcctga ttaatcgacg ttctccaatc taggcctaat acatcaattg
     5581 gcaaatcatt ccattcattg attaaatgac tggcacctac accgaataaa attaccggca
     5641 catcatgttt ttctttaacc tcactgatta atcgaatcat atgtggttta atgtaacgtc
     5701 tgtaatcctc gacatttaat gcacctaccc atgaatcgaa aatttgaatc aattcggcac
     5761 ctgcttcgac ttgagctgtt acatatttaa cagatacatc aactaaatga ttcattaaag
     5821 caaaccatgt tgcttcatct ctatacatca tcgcttttgt aaaattgtaa tttttcgatg
     5881 gtccgccttc aatcatatat gacgctaatg taaatggtgc cccagtaaat cctattagcg
     5941 gcacatttaa cttttcttct gttaaaagtt taattgtatc taatacatat ggtacatctc
     6001 gttcggggtc tatttgagaa agtttctcaa catcttgaat tgttttgata ggattatgaa
     6061 tcactggacc aatacccgat ttaatttcta catcgacacc aattggcttt aatggtgtca
     6121 taatatcttt gtataaaatt gctgcatctg tatgataatt atcaactggt aaatgtgtta
     6181 cataagcgca caactccggc tgatgtgtaa tatcgaatag tgaatatttt tctttcaatt
     6241 ttcgatattc tggttgcgaa cggccagctt gtcgcataaa ccaaacaggt gtatgtgtcg
     6301 tttcttcacc tttaatcatt ttcaaaattg tattgttttt attatgcacc ataaaggcct
     6361 cctaaattaa aatcattctt atctatatta tcatatcgct cattcgttcg tattttcaat
     6421 aaataaatgt cataaaactg acattaaatt acacatcatt tgtttgtgta tttaaatttt
     6481 aaaatcttga tggattatct ccatatcttt ttgtcttgca tgatttatta tagtaacttt
     6541 aaccgtatat taatagtggt aatgactagt ttatcgtcgt ataataaata aaaacataag
     6601 ggggaccttt catatgaaga aattatatac atcttatggc acatatggat ttttaaatca
     6661 gataaaaatc aataatccat ctcatcattt attccaattt tcaactactg attcatcggt
     6721 gatttttgaa gagactgaag aaaatacagt actgaaatca ccttcaatat atgaagttat
     6781 taaagaaata ggcgcattca atgaagatca tttttattgc gcaattttta ttccgtcaac
     6841 tgaagaccat gtttatcaac ttgaaaagaa attaatcagt gttgatgaca atttcaaaaa
     6901 ctttggtggc tttaaaagtt atcgattgct gagacctgtt aaaggtacaa catacaaaat
     6961 ttactttggc tttgctgatc gacaaactta tgaagacttt aaaaattctg atgcttttaa
     7021 agatcatttt tcaaaagaag cattaagtca ttactttggt tcaagcggac aacattcaag
     7081 ttattttgaa agatatttat acccaataaa agaatagatt cgaagaaaac ttccattggt
     7141 tatgctgtta acgtatagat gctaatgtaa tctgaacgcc gtatcatttg gcgttcaaca
     7201 ttacatgcac ctttacaaca tacatgccaa tggaagtttt ttagtctcgt aataatgttt
     7261 cctgatattt taacttttta ataatacttc taatcgttaa taaacctaca atatagaaaa
     7321 tgagtacaac atagaataac gtcatatgtt ttataataaa tgccactgca aacacagtac
     7381 aaataacaaa cattaatcta tataaaaact gttcataccc tttgattacc ttctcttcag
     7441 gtacgggcca cacttgaggc cataagccat atgcttgctg tgaataaaat tgtgccattt
     7501 gtaacaatat aatatataca aataaacacc caataattgc tgtcactaat ggatatgata
     7561 accaaagcat aagtaaaact gcaataatta ctaacctaaa gataatatta aatgcgtctc
     7621 tccctcttat aaagcttcta ataaataaga ataaatacat cgcattagag ttaaatttac
     7681 taccctttgg aactggtaaa agtatatcta gataacttct tctgactgca gattctttca
     7741 aatgttttac atcggtgaac atattaacaa atttataata attcatatga tgtcgatgtt
     7801 cgatagcaat cattttctcc caaggatata aaaagccagg tttatatttt ttaactaaaa
     7861 attctattaa cacaggcaaa gcaaccatca caaatgcgat gtaccatttt ggagctaaca
     7921 gtaagtaata tgttagagca aagatgatga atgatattaa attaacttgc catgttttaa
     7981 gtcccgattg ataccattgc catcttatgc gtaaaccaac atatggaaaa attaatgcac
     8041 tgactccaaa acaaatgtaa aatgccacat tatgttgatt aatattataa aacaacggga
     8101 acatcacaat aacaataatg agttggatta atatgcgcgc aaagtaacta tataaaatcg
     8161 catgacgcat aaattgagac atgtgttttt caaatggtaa taaaaagatt ttatccgctt
     8221 cttttaggag tggtcgcatt ggaaaaatgg atgtcaacgc aacaatcact gctgctatta
     8281 atgcaaaatt gatattcgtt ggaatgtgtt ttaaccattc gccatatcca aaaataaatg
     8341 cacccagcaa aataagtaaa aagaccatga aatgaccatt aaatataaac ttattataat
     8401 aatttttctc tttacgaagg gcatgtaatc ttttattaaa taaagtggtc gcttggttac
     8461 gcatgtacat ctccaccttg cgtcacatga atatatatat cgtctaatgt ttgattatgt
     8521 aagccagttt gttgtctcaa tgcttctaaa tctccaaatg caacgacttc accttcgtct
     8581 agtatgataa aacgatcaca gtaacgttca gctgttgcta aaatatgtgt actcattaga
     8641 acggttctac cttcgttttt cttttcaacc attaaatcta acatggattg aattcctaat
     8701 ggatctaggc caaggaatgg ttcgtctata atatacaatt cgggattaac gataaacgca
     8761 caaataatca tgactttttg tttcatcccc ttagaaaaat gactcggaaa aactttcaat
     8821 tcattttcta aacggaatgt ctttaataat ggcattgctc gattcatcgc ttcatcacga
     8881 tcaatatcat atgccattgc tgtcatctca atgtgttcct ctaatgtgag ttcttcataa
     8941 ataaccggtg attccggaat ataagataac tttcttctat aagcctctat gtcatcatta
     9001 atgttgatat ctgaaattga tagagatcct tccataggtg taagcaatcc tagcatatgt
     9061 ttaatcgttg tactcttacc agcgccatta aggccaataa gtccaacaat ttcgcctttg
     9121 tttaattcaa aatttatatc tttaattaca gggcgttttc catatccacc tgtaagctgt
     9181 tctactttaa ctgtcataag gcacctccat gacttatatt gtaccaaaaa ttataaaatg
     9241 ctcatattaa atacacatgt cctaatatcg aatttttagc gacaatgtta taatgaatgg
     9301 taatactagt tgaaaaggag tgtagtcatc atgtcagaaa caattttcgg caaaatttta
     9361 actggagaaa ttcctagctt taaagtatat gaagacgatt atgtctatgc ctttttagat
     9421 atatcacaag ttactaaagg acatacgtta ttaattccta aaaaagcttc tgctaatatc
     9481 tttgaaactg atgaagaaac aatgaaacat atcggtgcag cattacctaa agtagcaaat
     9541 gctattaagc gtgcatttaa tcctgatggt ttaaacatta ttcaaaataa tggtgagttt
     9601 gcagatcaat ctgtatttca tattcatttc cacttaattc ctcgatatga aaatgatatt
     9661 gatggatttg gttataagtg ggaaacacat gaagacattt tagataacga tgcaaaacaa
     9721 caaattgctg aacaaattca agcacaattt taaatgtatg cttaatctaa gctcgaacgg
     9781 gtataatatg attaatatta taacaattgc gtttgaagtg ataacatcaa ggttagcaat
     9841 tttaaacaaa atgagttatc aagataacag atgataaaag tgaggagaat ataaatgaaa
     9901 gcatcacgca ttctattcgg tatcggtgtt ggtgtagcag ctggttttgt agttgcactt
     9961 caaggacgag acgacaaaag tgtcaagaac aacacgatcg atcgtactgc ccctactggt
    10021 tcaaaatcag aactacaacg tgaatttgaa acgattaaac aaagttttaa tgacatttta
    10081 aactatggtg ttcaaattaa aaacgaaagt gcggaatttg gtagttcaat tggtggtgaa
    10141 attaagtcat tacttggaaa cttcaaatct gacatcaatc ctaatattga acgtttacag
    10201 tcacacatcg aaaatttaca aaatcgtggc gaggatattg gaaacgaaat ttctaagtag
    10261 caggttacgt tctcgatcac aactattttt attagtaact gcatatttat tttttaaatt
    10321 taaatgccaa ataaacgaga tgacattaga aattagatat ttcttgtcat ctctttttta
    10381 tggctcgaat gaacatttga ttacaaatta caggaataca ttgtttgtag tgattttcgc
    10441 ttaaatcata tttataaatt gattgaaaac attgcttagg attcattgtg ttatccttgc
    10501 actttgatta cgctttactt aaatcattat cgacaaacaa catacttata ttttcattga
    10561 gccgaacctt atatatacat tacatatacc ttacttgcac aaattattaa tctggtgttt
    10621 gttataatta catatcacta tatttttagc atttgtatga cttaattggt caaaagatgc
    10681 ttttgcatat gccctgccat atccattttt tatattattc aagttttcaa atactcatgt
    10741 cacacgcgcc tttttcataa gtgatgcttt attagcaaga atatgtgttc gcagaaattt
    10801 gttctgcatt ctacttctac gctagtcaat cagccaattt taccaatccc cactttcgcg
    10861 tttcaaatca aacaatacgt cgccccttac ttcatatata acaattcttc taacatgata
    10921 tgttactatt gaattactga acctgagtta gttataatct aacttatatt gaaaagagat
    10981 gaggcgtaag atatgtttct atgtaaaaga caaattgata tcaatgcacg atttggtttg
    11041 cctagaattg catttatgag tgcagttgca accatcatta tgtttttagt tagttatgaa
    11101 gtaatgtatt ttttatctaa tacgccatta tcagatagac attttctcat ctttttatta
    11161 cttgtattta tgacgtatcc attacataaa agtatacatt tattattttt cttaccatat
    11221 agaaaatcgt ttaaagttca taagttgact aaaagaaaat ggcttatatt ctataatacc
    11281 tacgtcaatc aacctgtaca caaattttat ttttgcatta acttaatatt gccgttaatt
    11341 atcttatctg caatgttcgt ttatctaaca atttcatttc cgcaatatgg acattatttt
    11401 atgttcttat tggcattgaa tttcggtatt tccattacag atttattata tttaaaaata
    11461 attatatttt cta
//
LOCUS       DAHHOJ010000083        11262 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.083, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000083 DAHHOJ010000000
VERSION     DAHHOJ010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11262
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.083"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            49..744
                     /locus_tag="NBH32_001932"
                     /pseudo
     CDS             49..744
                     /locus_tag="NBH32_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501278.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
     gene            746..1516
                     /locus_tag="NBH32_001933"
     CDS             746..1516
                     /locus_tag="NBH32_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515515.1"
                     /translation="MGTLIKQECFKLFKKKSTFIAPIVFILLMVAQGYIATKYNEIFT
                     PQESFTSAYNGFSWFAFLLIIQASTIISMEFHYGTIKNLLYREYSRTTMIVSKIITLF
                     IISLIYFVITIIASIVIGSLFFNDLNIFESSGNQLSLLNQLLLVSLGTFVGVWLVLSL
                     TLLLSSATNSTGVAIAVGIVFYFASSILAVIQTALLEKIDWLKWNPINMMNIMLQTVE
                     KGFSKSTKLELHELFIGNIAYISIFLILVVFIFKKKNI"
     gene            1953..3917
                     /locus_tag="NBH32_001934"
     CDS             1953..3917
                     /locus_tag="NBH32_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HCD1515516.1"
                     /translation="MTQITERELKKKYLDLLSQHFDTPEKLATEIINLESILELPKGT
                     EHFVSDLHGEYEAFQHVLRNGSGNVRAKINDIFKDKLSTKELNDLTALVYYPEDKLQL
                     IKCDFQNYGQLNVWYITTIEHLIQLIKYCSSKYTRSKLRRALPEQYVFIVEELLYKNN
                     EFKNKKSYYETLVNQVIELKQADDLIIGLAYSVQRLVVDHLHVVGDIYDRGPQPDKIM
                     DTLINYHSLDIQWGNHDVLWVGAYAGSKVCLANLLRICARYDNLDIVEDAYGINLRPL
                     LTLAEKYYDADNPAFKPKKRPDKHERLTQREESQITKIHQAIAMIQFKLEIPVIKRRP
                     NFEMDERLVLEKVNYDTNEITVYGKTYPLKDTCFQTVNRDNPAELLPEEEEVMNKLLL
                     SFQQSEKLRRHMSFLMRKGSLYLPYNGNLLIHGCIPVDENGEMESFEIDGQTYSGQEL
                     LDVFEYHVRKSFDEKENTDDLSTDLVWYLWTGKYSSLFGKRAMTTFERYFIADKASHK
                     EEKNPYYHLREDVNMVRKMLSDFGLNPDEGRIINGHTPVKEINGEDPIKADGKMLVID
                     GGFSKAYQSTTGIAGYTLLYNSFGMQLVAHQQFNAKEKILSEGIDELSIKRIVDKELQ
                     RKKIRNTNKGKELQAQIDILKMLMHDRYLD"
     gene            4259..5338
                     /locus_tag="NBH32_001935"
     CDS             4259..5338
                     /locus_tag="NBH32_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515517.1"
                     /translation="MRILKESIIVAFAFVGVVVGAGFATGQEIFQFFTSHGVYSISGI
                     IVTGLLITLGGMIVMHTGHHLKSRNHSDSINYFLYPSIARGFDIILTMFMLSLAIIMT
                     AGGASTIHQSFNLPYWLSALILVAFILATLFLKFDRLIAVLGGVTPFLIAIVIMIAVY
                     YFTTSHLDFTAANNDAQIHKQKSLSPGWWFDAINYASLQIAAAFSFLSVMGSKVKYRD
                     STLYGGLIGGLIITFLLMMINLGLISQFDKIKHVDLPTLKLATQMSPSIGIIMSVIMI
                     LVIYNTVVGLMYAFASRFSVPFSRRYFIIIITMAVITYISTFIGFISLIGKVFPIMGL
                     FGFILLIPVLYKGLIKRITGKSHID"
     gene            complement(5443..6036)
                     /locus_tag="NBH32_001936"
     CDS             complement(5443..6036)
                     /locus_tag="NBH32_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1515518.1"
                     /translation="MEHIFREGQKGAPTLILLHGTGGDEFDLLPLGEALNENYHLLSI
                     RGQVSENGMNRYFKRLGEGVYDEEDLAFRGQELLTFIKEAAERYEFDIEKAVLVGFSN
                     GSNIAINLMLRSEAPFKKALLYAPLYPIEVTSTKNLSDVSVLLSMGKHDPIVPLAASE
                     QVINLFKTRGAQVEEVWVNGHEITETGLTAGQQILGK"
     gene            complement(6058..7026)
                     /locus_tag="NBH32_001937"
     CDS             complement(6058..7026)
                     /locus_tag="NBH32_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000183732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HCD1515519.1"
                     /translation="MANNHELLGIHHVTAITDNAERNYKFFTEVLGMRLVKKTVNQDD
                     IYTYHTFFADDVGSAGTDMTFFDFPDIPKGQAGTNSITRPSFRVPNDDALTYYEQRFD
                     EFGVKHEGIQELFGKKVLPFEEVDGQVYQLISDELNEGVAPGVPWKNGPVPVDKAIYG
                     LGPIEIKVSYFDDFKNILETVYGMTTIAHEDNVALLEVGEGGNGGQVILIKDDKGPAA
                     RQGYGEVHHVSFRVKDHDAIEAWATKYKEVGINNSGIINRFYFEALYARVGHILIEIS
                     TDGPGFMEDEPYETLGEGLSLPPFLENKREYIESEVRPFNTKRQHD"
     gene            complement(7104..7538)
                     /locus_tag="NBH32_001938"
     CDS             complement(7104..7538)
                     /locus_tag="NBH32_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1515520.1"
                     /translation="MDRTKQSLNVFVGMNRALDTLEQITKEDVKRYGLNITEFAVLEL
                     LYNKGPQPIQRIRDRVLIASSSISYVVSQLEDKGWITREKDKDDKRVYMACLTEKGQS
                     QMADIFPKHAETLTKAFDVLTKDELTILQQAFKKLSAQSTEV"
     gene            7765..8049
                     /locus_tag="NBH32_001939"
     CDS             7765..8049
                     /locus_tag="NBH32_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1515521.1"
                     /translation="MSNLEIKQGENKFYIDDDENHALAEITYRFVDNNEINIDHTGVS
                     DELGGQGVGKKLVKAVVEHARENHLKIIASCSFAKHMLEKEDSYQDVYLG"
     gene            8223..9029
                     /locus_tag="NBH32_001940"
     CDS             8223..9029
                     /locus_tag="NBH32_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1515522.1"
                     /translation="MAFHDKTATQVTNIELNVRDLDLMTTFYKNILGLSVKSSDDNTT
                     VLSVGTGGHTLTLHLLEDGRQTSPREAGLFHIAFLLPTTEDLANFLYFVAQKNMGIGA
                     GDHLVSEALYFNDPEGNGIEVYRDRPSSSWEWQNGKVKMDTLEVDSQTLLTHRTDEGW
                     QGMPAKGMIGHLHLKTHDLDAAYQCYIEQLGFQHVSDFPRALFMSTNHYHHHIAANTW
                     QSNQARQNNAQSYGLTHVDIYQPDASTHTFTAPEGFGITVHSNTDLVPEK"
     gene            complement(9149..9820)
                     /locus_tag="NBH32_001941"
     CDS             complement(9149..9820)
                     /locus_tag="NBH32_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1515523.1"
                     /translation="MSNMNQTIMDAFHFRHATKQFDPQKKVSKEDFETILESGRLSPS
                     SLGLEPWKFVVIQDQALRDELKAHSWGAAKQLDTASHFVLIFARKNVTSRSPYVQHML
                     RDIKKYEAQTIPAVEQKFDAFQADFHISDNDQALYDWSSKQTYIALGNMMTTAALLGI
                     DSCPMEGFSLDTVTDILANKGILDTEQFGLSVMVAFGYRQQDPPKNKTRQAYEDVIEW
                     VGPKE"
     gene            10090..11082
                     /locus_tag="NBH32_001942"
     CDS             10090..11082
                     /locus_tag="NBH32_001942"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000161541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCD1515524.1"
                     /translation="MTKIMFFGTRDYEKEMALNWGKKNNVEVTTSKELLSSATVDQLK
                     DYDGVTTMQFGKLENDVYPKLESYGIKQIAQRTAGFDMYDLDLAKKHNIVISNVPSYS
                     PETIAEYSVSIALQLVRRFPDIERRVQAHDFTWQAEIMSKPVKNMTVAIIGTGRIGAA
                     TAKIYAGFGATITAYDAYPNKDLDFLTYKDSVKEAIKDADIISLHVPANKESYHLFDK
                     AMFNHVKKGAILVNAARGAVINTPDLIAAVNDGTLLGAAIDTYENEAAYFTNDWTNKD
                     IDDKTLLELIEHERILVTPHIAFFSDEAVQNLVEGGLNAALSVINTGTCETRLN"
BASE COUNT         3676 a         1980 c         1804 g         3802 t
ORIGIN      
        1 tataaaatct taagattcag attatttaat agcaaaggag atagtgatat ggatgtttta
       61 acaatagaac atttaacaaa gaagataggc aacaaaacga ttctcgaaga tgtatcattt
      121 aagctgaaac gcggacaaat agttggtctt gttggagcga atggtgcagg taaaacaact
      181 ttaatgaaag ttatattagg ttactctagt ttccaaagcg ggaattttaa tgttattaac
      241 agcaaggaca gcaaaagcaa tatcggtgca ttgattgaaa atccaggaat atatcctttt
      301 atgtctggat atgaaaactt gaagttattg aactaatcaa aaaacactca agatatcgat
      361 aaaattgtct cacaacttca tatggatgaa tacattcata aaaaagctaa aacgtattct
      421 cttggtatga aacaaaaatt aggaattgct atagcatttt taaataaacc tcaattcatt
      481 atcttagatg aaccaatgaa cggcttagat ccaaaagctg tgcgagatgt acgtgaattg
      541 attgtacaaa aagcgcaaga aggtgtcact ttcttaattt cgagtcatat tttaagtgag
      601 ttagttaaaa tcacaaactc tatccttatt attaacaaag gtaaaattgt tacagaaaca
      661 tcggaagaag aacttaaaca atttaaagat aatgatttag aaaatgtatt actagaaatc
      721 atagaaaggg aggatcaagc ataaaatggg aactttaatt aaacaagaat gtttcaaatt
      781 atttaaaaag aaatcaactt ttatcgcacc tattgtcttt attctactaa tggttgctca
      841 aggttatatc gctacaaaat acaatgaaat ttttacgcca caggaatctt ttacatctgc
      901 ttataatggt ttttcatggt ttgcattttt attaattatt caagcaagta caatcatttc
      961 aatggaattt cattacggta cgattaaaaa tttactctat cgtgaatatt caagaacaac
     1021 tatgattgtt agcaaaatca tcacattatt tattatttct ttaatttatt ttgttattac
     1081 aattattgct tcaattgtta ttgggtcttt attctttaat gatttaaata tatttgaaag
     1141 tagcggtaat caattatctt tattgaatca attattatta gttagtttag gcacatttgt
     1201 tggcgtttgg ttagttttaa gcttaacgtt gctattatca tctgcaacaa attcaacggg
     1261 tgtagccatt gctgtaggta ttgtttttta ttttgcaagt tctattttag cagttattca
     1321 aacggcactt ttagaaaaaa tagactggct aaagtggaat cctattaata tgatgaatat
     1381 tatgcttcaa acagttgaaa aaggctttag taagtcgaca aaattagaac ttcatgaatt
     1441 gtttattggt aatattgctt atatttctat tttcttaata cttgtagtat ttattttcaa
     1501 gaagaaaaat atttagtaac ttaaagtatt agatgaataa atacacacag attccatcgt
     1561 aattcaaaat cattttcaaa tcccttcacc ataatggtgc ggggattttt gatatgtcag
     1621 cataccactc attctatctt ttatatattc cagaatcccc ccacttgtaa tgtacaaaat
     1681 atgctcactt atacgataat cgtatctctg ccaagctatt tcaactctgc ttcaacaact
     1741 ttatcacttc acatttatgc gggtacactt caacgctact aatatgaatg atacaaccat
     1801 agattctcaa cactaaaatt cacaatatgt ttactctatt tttatcaatt caagcatact
     1861 tattgaaaaa tgttaacgtt ttcttgataa tcattgtaag cgtattaatt ttacaaacca
     1921 atgattgaaa tatactacag gagtgacaca caatgactca aattactgaa agagaattaa
     1981 aaaagaaata tttggattta ttatctcaac atttcgatac tccagaaaag cttgcgactg
     2041 aaattattaa tttagaatct attctagaat tgcctaaagg tacggagcat ttcgtcagcg
     2101 atttacatgg tgaatatgaa gctttccaac acgtattacg caacggttct gggaacgtgc
     2161 gagcgaaaat caatgatatt ttcaaagaca agttatcaac gaaagaactt aacgatttga
     2221 ctgcccttgt ttactatcca gaagacaaat tgcagcttat taaatgcgat tttcaaaatt
     2281 acggacaatt aaatgtttgg tatattacga cgattgaaca tctcattcag ctaattaaat
     2341 attgttcttc aaaatatacg cgttcaaaac ttcgacgtgc cttgccggag caatacgttt
     2401 tcatcgtaga agagttatta tataaaaaca acgaattcaa aaataaaaaa tcttactacg
     2461 aaacacttgt aaatcaagtg attgagctta agcaagcgga tgatttaatt atagggcttg
     2521 cgtattctgt acaacgctta gtcgtcgatc atttacacgt tgtcggcgat atttatgatc
     2581 gtggcccgca accagataaa atcatggaca cgctgattaa ttatcattct ctggatattc
     2641 aatggggtaa tcatgatgtg ctatgggttg gtgcttatgc agggtctaaa gtatgtttag
     2701 ctaatttact acgtatttgt gcacgttatg acaatctaga tatcgttgaa gatgcttacg
     2761 gtattaattt aagacctttg ctaacattag ccgaaaaata ctatgatgca gataatcctg
     2821 cttttaaacc taaaaaaaga cctgataaac atgaacgttt aacacaacgc gaagaaagtc
     2881 agattactaa aattcatcaa gctattgcga tgattcaatt caaactggaa ataccggtta
     2941 ttaagcgccg tccaaacttt gaaatggacg aacgacttgt acttgaaaaa gttaattacg
     3001 atactaatga aataacagta tacggaaaaa catatccatt aaaagataca tgcttccaaa
     3061 ctgttaaccg tgataaccca gcagaattac tacctgaaga agaagaagtc atgaataaac
     3121 tattattgtc attccagcaa tctgaaaaat tacgtcgtca tatgtctttc ttgatgcgta
     3181 aaggctcact ttacttacct tataatggca atttactcat tcatggttgt attccagttg
     3241 atgaaaatgg tgagatggaa tcattcgaaa ttgatggtca gacatacagt ggccaagagt
     3301 tattagatgt gtttgaatat catgtccgta aatcatttga tgaaaaagaa aatactgatg
     3361 acttatccac tgatttagtt tggtatttat ggactgggaa atattcttca ctatttggta
     3421 aacgtgcgat gactacgttt gagcgatatt ttattgcaga taaagcttct cataaagaag
     3481 aaaaaaaccc gtactatcat ctacgtgaag atgtgaatat ggttcgtaaa atgctcagtg
     3541 attttggatt aaatccagat gaaggacgca ttattaatgg tcacacacca gtgaaagaaa
     3601 ttaatggcga agatcctatc aaggctgatg gaaaaatgct tgtcattgat ggtggctttt
     3661 caaaagctta tcagtcaaca actggcattg caggttatac attgttgtat aattcattcg
     3721 gaatgcaact cgttgcacat caacaattta acgcaaaaga aaaaattctt tccgaaggta
     3781 tcgacgaact gtcaattaaa cggattgttg ataaagaatt gcaacgtaaa aaaatccgta
     3841 atacaaataa aggtaaagag cttcaagctc aaatagatat tttgaaaatg ttaatgcatg
     3901 atcgttactt agactaaaga cgacatttgg tgcatgcatc gtgcacttac ttaatggtac
     3961 tttgtttacg cattggctac gcaaacgcga tggtaaatag tcgacctaac tacaccgaaa
     4021 aatcccctca ctattgcgag tagtgagggg attggtgtat ctatgctagt tcctgttaac
     4081 tgtattataa caacttcaca gcaaaaaaat gcaaaatcaa ctaataaatt aatgaaatga
     4141 ccgacgccct cgcttctact ccaatttaac aaaatactat attagtacta tatttttcat
     4201 taacatgtat gttacatttt ctatattact taaaattacg aagggacatt tttaaatcat
     4261 gcgtatctta aaagagtcca ttattgtggc atttgccttt gttggtgttg tcgttggtgc
     4321 cggctttgct actggtcaag aaattttcca gtttttcaca agtcatggcg tgtatagcat
     4381 ttcaggcatt attgtgacag gactattgat tactttaggt ggaatgattg tcatgcatac
     4441 aggtcatcat ctaaagtcta gaaatcattc tgattcaatt aactatttct tatatccctc
     4501 tattgcaaga ggttttgata ttattttgac aatgtttatg ttgtctttag ctattattat
     4561 gactgcaggt ggcgcttcaa ccattcatca aagtttcaac ttaccgtatt ggctgagcgc
     4621 actcatatta gtcgccttta ttttagcaac actgtttcta aaattcgatc gtttaattgc
     4681 tgtgcttggc ggtgttaccc catttttaat tgcgattgtc attatgattg cggtctacta
     4741 tttcacaacg agtcatcttg attttactgc cgctaataat gatgctcaga ttcataagca
     4801 gaaatcatta tcacctggat ggtggtttga tgcaattaac tatgcaagct tgcaaattgc
     4861 tgctgccttc agcttcttat cagtgatggg tagtaaagtt aagtatcgtg actcaacgtt
     4921 atacgggggc ttgattggcg gtttaatcat tacattttta ctcatgatga ttaacctagg
     4981 tttaatttct caattcgata aaattaaaca cgtcgactta cctacattaa aattagcgac
     5041 acaaatgtct ccgtcaatcg gtattattat gtctgtcatt atgatacttg tcatctacaa
     5101 tactgttgtt ggattaatgt atgcatttgc gtcacgtttc agcgttccat ttagcagacg
     5161 ttacttcatc attattatta caatggctgt catcacttat attagtacat ttatcggttt
     5221 catttcatta attggaaaag tattccctat tatgggattg ttcggtttca tcttactcat
     5281 acctgtactc tataaaggtt taattaagcg tattaccggc aaatctcata tcgattaaaa
     5341 tatctatact attgtaaaat ttagtacaac acttcaaaat caaaaaagcg ggcagatgaa
     5401 aagtaaaaat cttttccatc tgtccgcttc ttaatagaac tgttatttcc caagtatttg
     5461 ttgaccagcc gttaatccag tttctgtaat ttcatgacca ttcacccaaa cttcttcgac
     5521 ttgtgcccca cgtgtcttaa acaagttaat gacttgttca cttgcagcta atggtacaat
     5581 tggatcatgt ttccccatag aaagcaacac actgacatct gataaattct tcgttgacgt
     5641 tacttcaatt ggatataacg gtgcatataa caatgctttt ttaaatggtg cttctgaacg
     5701 caacattaag ttaattgcta tatttgatcc atttgaaaat ccaacaagca cagccttttc
     5761 aatatcaaat tcataacgtt cagcagcttc tttaatgaac gttaacaatt cttgtccacg
     5821 aaatgccaaa tcttcttcat cataaacacc ttcaccaaga cgtttgaaat aacggttcat
     5881 cccattttct gaaacttgtc ctctaatact taacaagtga taattttcat tcaatgcttc
     5941 gcctaatggc aataaatcga actcatcacc acctgtacca tgcaacaata ttagcgttgg
     6001 cgcacctttt tgtccttcac gaaaaatatg ttccatcaca aatcctcctc attccaatta
     6061 atcatgttga cgcttcgtat taaaaggtct aacttccgat tctatatatt ctcttttatt
     6121 ttctaaaaat ggtggtaatg ataacccttc gcctaatgtt tcataaggtt catcttccat
     6181 aaatcctggt ccatctgttg aaatttctat taaaatatgc cccacacgtg catataatgc
     6241 ttcaaaatag aaacgattaa tgatgcctga gttgttaata cctacctctt tatatttcgt
     6301 tgcccacgct tctattgcat catgatcttt cacacgaaat gacacatgat gtacctcacc
     6361 ataaccttga cgtgctgctg gccctttatc atcttttatt aagattacct ggccaccatt
     6421 gcctccttcg ccaacttcaa gtaatgcgac attatcttca tgcgcaatag ttgtcatacc
     6481 gtaaacagtc tctaaaatat ttttaaagtc gtcaaaataa cttactttaa tttcaatggg
     6541 tcctaatcca taaatcgctt tatctactgg aaccggtcca ttcttccaag gtacaccagg
     6601 tgctacacct tcatttaact catctgaaat taattgatac acttggccat cgacttcttc
     6661 aaatggcaac acttttttac caaatagttc ttgaatacct tcgtgtttga caccaaactc
     6721 atcaaagcgc tgttcataat atgttaatgc gtcatcgtta ggcactctaa aagacggtct
     6781 tgtaatagaa tttgttccag cctgcccttt aggaatatct gggaaatcga agaatgtcat
     6841 atctgtacct gctgaaccta catcatctgc aaaaaatgta tgatacgtat aaatatcatc
     6901 ttgattgact gtctttttaa ctaagcgcat gcctagtact tctgtaaaaa atttataatt
     6961 acgttctgca ttatctgtta ttgcagtaac atggtgaata cctaataatt catgattatt
     7021 tgccatttta tataccctct ttgtcttttt agtgtattta tctcgaaatc gagatatttt
     7081 aatgtaaatt tttagtgcac gccttacact tctgtagatt gtgcacttag tttcttaaac
     7141 gcttgttgta agattgttaa ttcatccttt gttaacacat caaacgcttt tgttaatgtc
     7201 tcagcatgct tagggaaaat atctgccatt tgactttgac ctttttcagt taaacaagcc
     7261 atatatacac gtttatcatc tttatccttt tcacgtgtaa tccaaccttt gtcctctaat
     7321 tgacttacaa catatgaaat gctgctactt gcaattaata cgcggtctct aatacgttga
     7381 attggttgcg gacctttatt ataaagcaac tcaagcactg caaattcagt aatatttaat
     7441 ccataacgtt ttacgtcttc ttttgtaatt tgctctaatg tgtctaatgc tctattcatt
     7501 ccgacaaaga cattgagcga ttgtttcgtt cgatccatat catcaccaac tttttcattt
     7561 tatttcgatt tcgagataaa ttatatagct gaatagattt aatgtcaaga tacgtgtttt
     7621 catctttatt tcaaactgcg cttaataaaa acgactacat aaatgtggca atgtcgtcac
     7681 atgactgtca tgcacctttg acttcgttta agttaaaata gaatagggaa atactataat
     7741 caatcatgat aggagttgat atgtatgagt aaccttgaaa ttaaacaagg cgagaacaaa
     7801 ttctatattg atgatgatga aaatcatgct ttagctgaaa tcacataccg ttttgtggat
     7861 aataatgaaa ttaacattga tcatacaggc gtatctgatg aactcggtgg tcaaggtgtt
     7921 ggcaaaaaac tagttaaagc agttgttgaa catgctcgcg aaaatcattt aaaaatcatt
     7981 gcctcatgtt catttgccaa acatatgtta gaaaaagaag attcatatca agatgtatat
     8041 cttggttaaa taaattttgc gatggtgcaa ctgtgccatc gctttttatt tgttcgcatc
     8101 aatataaaat tgcaaaacga ctagttattt tgcatttcaa actttatgct gtcaattcat
     8161 tttcttttct ggtatacttt ttatactaaa ttatagaaaa atagaaaaag aggcgatgca
     8221 taatggcatt tcacgataaa acagcaacac aagtgacaaa cattgaactg aacgtaagag
     8281 acttagactt aatgacaaca ttttacaaaa acattttagg actatccgtt aaaagttctg
     8341 acgataacac aaccgtatta tctgttggga ctggtggcca tactctaacg ttacatttat
     8401 tagaagacgg ccgtcagact tccccacgtg aagcaggact ttttcatata gcatttttat
     8461 taccaactac tgaggatcta gctaacttct tatatttcgt ggcacaaaaa aatatgggca
     8521 ttggcgctgg tgatcattta gtcagtgaag ctttatattt caacgacccc gaaggtaatg
     8581 gtattgaagt ctatcgcgat agaccttcat cttcatggga atggcaaaat ggcaaagtta
     8641 aaatggatac tttagaagtt gatagccaaa ctttattaac acatcgtact gatgaaggtt
     8701 ggcaaggaat gccagcaaaa ggcatgattg gacacttaca tttaaaaaca catgatttag
     8761 acgcagcata tcaatgttac attgaacaat taggattcca acatgtgtct gattttccac
     8821 gtgcgctatt tatgtcaacg aatcactatc accatcatat tgccgctaac acttggcaat
     8881 ctaatcaagc aagacaaaac aatgcacaaa gttatggatt aacacatgtt gatatttatc
     8941 aacccgacgc ctcaacacat acatttacgg caccagaagg gtttggcatc acagttcatt
     9001 caaatactga ccttgttcca gaaaaataaa catccatact ttattaatac aattttttca
     9061 cgcaaaagcc tgcgattcat ttatgtaatc gcaggctttt ttattgctaa ctttttaatt
     9121 ttatatattt agacatacgg tattctattt attcttttgg tccaacccat tcaataacat
     9181 cttcataagc ttggcgtgtt ttatttttcg gtggatcttg ttgtctgtag ccaaatgcaa
     9241 ccatcactga taaaccaaat tgctcagtat ctaagatacc tttatttgct aaaatgtctg
     9301 tcactgtatc cagactaaaa ccttccatcg ggcatgaatc aatacctaat aatgcagctg
     9361 tcgtcatcat attgcctaat gcgatatacg tttgtttact tgaccaatca tacaaggctt
     9421 gatcattatc ggaaatatga aaatctgctt ggaatgcatc gaatttttgt tcaacagctg
     9481 gaatcgtttg tgcctcatat tttttaatat ctcttaacat atgttgtaca tacggtgatc
     9541 ttgacgttac atttttacgc gcaaaaatta gcacaaaatg gctcgctgta tctaattgct
     9601 ttgctgcgcc ccaactgtgc gcttttaatt catcacgtaa cgcttgatct tgaatcacga
     9661 caaacttcca aggttctaac ccaagagaac ttggagacaa tctacctgac tctaatattg
     9721 tttcaaaatc ttctttcgaa actttctttt gtggatcgaa ttgcttagtc gcatgtctga
     9781 aatgaaatgc atccataatt gtttgattca tattgctcat tgaacatagc ctccaatttt
     9841 tatatttatt actatacaaa ttatacttca tgttttaaaa agactaaagt aatccgtttg
     9901 caattcacta taacttgtgt acatgttata agttttctgc aataaaacac acttttgaat
     9961 tttggaaaat ttagtgttat aatatatttg tgatattttt cacaattaaa ttgtggcgtg
    10021 attttaatgt acataatctt taatttcact catttacttt tcaatctttt aaaagcgagg
    10081 tttttaataa tgacaaaaat tatgttcttt ggtacgcgtg attatgagaa agagatggca
    10141 ttaaattggg gaaaaaagaa taatgtcgaa gtaactactt ctaaagagct attatcaagt
    10201 gctacagtcg atcaattaaa agattacgat ggcgtaacta caatgcaatt tggtaagtta
    10261 gaaaatgacg tttatcctaa attagaatct tacggtatta aacaaattgc acaacgtact
    10321 gctggatttg atatgtatga tttagattta gctaaaaaac acaacattgt gatatctaac
    10381 gttcctagtt attcacctga aacaattgca gagtattctg tttctatcgc cctacaatta
    10441 gtgcgtcgct tcccagatat tgaacgccgt gtacaagcac atgattttac ttggcaagca
    10501 gaaatcatgt ctaaaccagt taaaaatatg actgttgcaa ttattggtac gggtcgtatc
    10561 ggtgctgcta cagctaaaat atatgcagga tttggtgcta caattacagc ttatgacgcc
    10621 tatcctaata aagatttaga ctttttaact tataaagata gtgttaaaga agctattaaa
    10681 gatgccgata ttatttcttt acatgttcca gcgaacaaag aaagctatca tttattcgat
    10741 aaagcaatgt ttaatcatgt gaaaaaaggt gcaatcttag ttaacgcagc acgtggtgca
    10801 gtcatcaata cacctgattt aatcgctgca gtgaacgatg gtactttgtt aggtgctgcg
    10861 attgatactt atgaaaatga agcagcatac ttcacaaatg actggactaa taaagacatt
    10921 gacgataaaa cattattaga gttaatcgaa catgaaagaa ttttagtaac accacatatt
    10981 gctttcttct ctgatgaagc agtacaaaac cttgttgaag gtggtttaaa cgcagcatta
    11041 tctgtcatta acactggtac atgtgaaaca cgtttaaatt aatatatatt taaacaggct
    11101 gggacataaa tccctaaaaa acagcagtaa gataattttc aattagaaga tatcttactg
    11161 ctgttctata tttatacaat acttcgtatt gaatggcttc gctatgccca tctggcacat
    11221 tactgtaaaa ttctataaat agaatttttt gatgatgggt cc
//
LOCUS       DAHHOJ010000084        11212 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.084, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000084 DAHHOJ010000000
VERSION     DAHHOJ010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11212
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.084"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            197..865
                     /gene="capA"
                     /locus_tag="NBH32_001943"
     CDS             197..865
                     /gene="capA"
                     /locus_tag="NBH32_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide type 5/8 biosynthesis
                     protein CapA"
                     /protein_id="HCD1515525.1"
                     /translation="MESTLELTKIKEVLQKNLKILIILPLLFLIISAIVTFFVLSPKY
                     QANTQILVNQTKGDNPQFMAQEVQSNIQLVNTYKEIVKSPRILDEVSKDLNDKYSPSK
                     LSSMLTITNQENTQLINIQVKSGHKEDSEKIANSFAKVTSKQIPKIMSVDNVSILSKA
                     DGTAVKVAPKTVVNLIGAFFLGLVVALIYIFFKVIFDKRIKDEEDVEKELGLPVLGSI
                     QKFN"
     gene            881..1567
                     /gene="cap8B"
                     /locus_tag="NBH32_001944"
     CDS             881..1567
                     /gene="cap8B"
                     /locus_tag="NBH32_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8B"
                     /protein_id="HCD1515526.1"
                     /translation="MSKKENTTTTLFVYEKPKSTISEKFRGIRSNIMFSKANGEVKRL
                     LVTSEKPGAGKSTVVSNVAITYAQAGYKTLIIDGDMRKPTQNYIFNEQNNNGLSNLII
                     GRTTMSEAITTTEIDNLDLLTAGPVPPNPTELIGSERFKELVDLFNKRYDIIIVDTPP
                     VNTVTDAQLYARAIKDSLLVIDSEKNDKNEVKKAKILMEKAGSNILGVILNKAKVDKS
                     SSYYHYYGDE"
     gene            1570..2334
                     /gene="cap8C"
                     /locus_tag="NBH32_001945"
     CDS             1570..2334
                     /gene="cap8C"
                     /locus_tag="NBH32_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8C"
                     /protein_id="HCD1515527.1"
                     /translation="MIDIHNHILPNIDDGPTNETEMLDLLKQATTQGVTEIIVTSHHL
                     HPRYTTPIEKVKSCLNHIESLEEVQALNLKFYYGQEIRITDQILNDIDRKVITGINDS
                     RYLLIEFPSNEVPHYTDQLFFELQSKGFVPIIAHPERNKAISQNLDILYDLINKGALS
                     QVTTASLAGISGKKIRKLAIQMIENNLTHFIGSDAHNTEIRPFLMKDLFNDKKLRDYY
                     EDMNGFISNAKLVVDDKKIPKRMPQQDYKQKRWFGL"
     gene            2354..4177
                     /gene="cap8D"
                     /locus_tag="NBH32_001946"
     CDS             2354..4177
                     /gene="cap8D"
                     /locus_tag="NBH32_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8D"
                     /protein_id="HCD1515528.1"
                     /translation="MAHLSVKLRLLILALIDSLIVTFSVFVSYYILEPYFKTYSVKLL
                     ILAAISLFISHHISAFIFNMYHRAWEYASVSELILIVKAVTTSIVITMVVVTIVTGNR
                     PFFRLYLITWMMHLILIGGSRLFWRIYRKYLGGKSFNKKPTLVVGAGQAGSMLIRQML
                     KSDEMKLEPVLAVDDDEHKRNITITEGVKVQGEIADIPELVRKYKIKKIIIAIPTIGQ
                     ERLKEINNICHMDGVELLKMPNIEDVMSGELEVNQLKKVEVEDLLGRDPVELDMDMIS
                     NELTNKTILVTGAGGSIGSEICRQVCNFYPERIILLGHGENSIYLINRELRNRFGKNV
                     DIVPIIADVQNRARMFEILEMYKPYAVYHAAAHKHVPLMEDNPEEAVRNNILGTKNTA
                     EAAKNAEVKKFVMISTDKAVNPPNVMGASKRIAEMIIQSLNDETHQTNFVAVRFGNVL
                     GSRGSVIPLFKSQIEEGGPVTVTHPEMTRYFMTIPEASRLVLQAGALAEGGEVFVLDM
                     GEPVKIVDLARNLIKLSGKKEDDIRITYTGIRSGEKMFEELMNKDEVHPEQVFEKIYR
                     GKVQHMKCNEVEAIIQDIVNDFSKEKIINYANGKKGDNYVR"
     gene            4167..5195
                     /gene="cap8E"
                     /locus_tag="NBH32_001947"
     CDS             4167..5195
                     /gene="cap8E"
                     /locus_tag="NBH32_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8E"
                     /protein_id="HCD1515529.1"
                     /translation="MFDDKILLITGGTGSFGNAVMKRFLDSNIKEIRIFSRDEKKQDD
                     IRKKYNNSKLKFYIGDVRDSQSVETAMRDVDYVFHAAALKQVPSCEFFPVEAVKTNII
                     GTENVLQSAIHQNVKKVICLSTDKAAYPINAMGISKAMMEKVFIAKSRNIRSEQTLIC
                     GTRYGNVMASRGSVIPLFIDKIKAGEPLTITDPDMTRFLMSLEDAVELVVHAFKHAET
                     GDIMVQKAPSSTVGDLATALLELFEADNAIEIIGTRHGEKKAETLLTREEYAQCEDMG
                     DYFRVPADSRDLNYSNYVETGNEKITQSYEYNSDNTHILTVEEIKEKLLTLEYVRNEL
                     NDYKASMR"
     gene            5208..6317
                     /gene="cap8F"
                     /locus_tag="NBH32_001948"
     CDS             5208..6317
                     /gene="cap8F"
                     /locus_tag="NBH32_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8F"
                     /protein_id="HCD1515530.1"
                     /translation="MNIVITGAKGFVGKNLKADLTSTTDYHIFEVHRQTKEEELESAL
                     LKADFVVHLAGVNRPEHDKEFSLGNVSYLDHVLDILTRNKKKPAILLSSSIQATQDNP
                     YGESKLQGEQLLREYSEEYGNTVYIYRWPNLFGKWCKPNYNSVIATFCYKIARNEEIQ
                     VNDRNVELTLNYVDDIVAEIKRAIEGTPTIENGVPTVPNVFKVTLGEIVDLLYKFKQS
                     RLDRTLPKLDNLFEKDLYSTYLSYLPSTDFSYPLLMNVDDRGSFTEFIKTPDRGQVSV
                     NISKPGITKGNHWHHTKNEKFLVVSGKGVIRFRHVNDDEIIEYYVSGDKLEVVDIPVG
                     YTHNIENLGDTDMVTIMWVNEMFDPNQPDTYFLEV"
     gene            6321..7445
                     /gene="cap8G"
                     /locus_tag="NBH32_001949"
     CDS             6321..7445
                     /gene="cap8G"
                     /locus_tag="NBH32_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 8 capsular polysaccharide synthesis protein
                     Cap8G"
                     /protein_id="HCD1515531.1"
                     /translation="MEKLKLMTIVGTRPEIIRLSSTIKACDQYFNQILVHTGQNYDYT
                     LNQIFFDDLELRQPDHYLEAVGSNLGETMGNIIAKTYDVLLREQPDALLILGDTNSCL
                     AAVSAKRLKIPVFHMEAGNRCFDQNVPEEINRKIVDHVSDVNLPYTEHSRRYLLDEGF
                     NKANIFVTGSPMTEVIEAHRDKINHSDVLNKLGLEPQQYILVSAHREENIDNEKNFKS
                     LMNAINDIAKKYKMPVIYSTHPRSWKKIEESKFEFDPLVKQLKPFGFFDYNALQKDAF
                     VVLSDSGTLSEESSILKFPGVLIRTSTERPEVLDKGTVIVGGITYNNLIQSVELAREM
                     QNNNEPMIDAIDYKDTNVSTKVVKIIQSYKDIINRNTWRK"
     gene            7448..8074
                     /locus_tag="NBH32_001950"
     CDS             7448..8074
                     /locus_tag="NBH32_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic acetyltransferase"
                     /protein_id="HCD1515532.1"
                     /translation="MRIAIEKIIGLLKNQSSKESNVKIHRLAYITNSKFDGNNYIDRW
                     CKIRNSHIGEYSYIGFGSDFNNVEVGRYCSISSDVKIGLGKHPTHFFSSSPIFYSNNN
                     PFNIKQKFIDFNDQPSRTTIKNDVWIGANVIIMDGLTINTGAVIAAGSVVTKNVGAYE
                     VVGGVPAKVIKKRFDNKTIEKLLESKWWEKTPDKLKGFSVEYLNKKDT"
     gene            8079..9188
                     /locus_tag="NBH32_001951"
     CDS             8079..9188
                     /locus_tag="NBH32_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HCD1515533.1"
                     /translation="MRILNIVSSNIVQDPRVLKQIETIKGVTNDYKIVGMNNSQATNK
                     RLENLDCNYRLLGSKVDPKNILSKLIKRIRFATGVIREIKAYKPDVIHANDFDVLLMV
                     YLSNYKKANIVYDAHEIYAKNAFINKVPLISKFVESIEKHIVKHRVNAFVTVSHAAKE
                     YYQSKGYKKEANVITNAPILNDSREFKEIENFKSIVYQGQIVMDRGYEEFIIASSAFK
                     QNAPSFIIRGFGPHEEVIKELISYNPENIRLDKPVEVKELVDKLAESNVGVVLTKPVS
                     INFEYTVSNKIFECIHAGLPVILSPVKEHIYLNEKYKFGIVLKEVTPLEIEKAVRKLR
                     DNHDLFNHLRQNAIKASKILNWQIESERLVKLYKF"
     gene            9202..10368
                     /locus_tag="NBH32_001952"
     CDS             9202..10368
                     /locus_tag="NBH32_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCD1515534.1"
                     /translation="MKFFVLCAIISMNIFIVISTFTKEVLGFPIEPVYYSTMVGIALI
                     TTVFAIYKIIVTQEIPRGLILLIAICLLYLAFYYFSPDKEEKLAKNNILFFLTWAVPA
                     AISGIYIKYINKATVERFFKLVFFIFSVSFIFVILIPKLTGEIPSYINFGLMNYQNAS
                     YLSAFTAGLGIYFIMKGSVKHKWIYVLFTIIDIPIVFIPGGRGGAILLILYGLFAFIL
                     ITFKRGIPIAVKSIMYISALSISSVLIYFLFTKGSNTRTFSYLQGGTLNLEGTSGRGP
                     IYEKGIYFIQQSPLLGYGPFNYYKLIGNIPHNIIIELILSFGLLGFFIIMICILLLVY
                     KMIRNYDPNTIDLLVMFIAIYPITLLMFSSNYLVVSEFWFVLFYFITKGRRHHG"
     gene            10361..>11212
                     /locus_tag="NBH32_001953"
     CDS             10361..>11212
                     /locus_tag="NBH32_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HCD1515535.1"
                     /translation="MVKKVFIMDSVKTIIGTLLIALGLQFLAYPIINQRVGNEAFGSI
                     LTIYTIITITSVVLGNTLNNIRLINMNLYKSNHYYWKFVLILLISILIESIALIIVFL
                     YFFNLNTIDIIFLILLNILMCLRIYLNVFFRMTLKYNQILYIALIQFLGLLIGLLLYY
                     LTQNWIVCFITSELFATIYTLVKLRGLTIGAYQSEDNNVVKDYVMLLSTNSLNNLNLY
                     LDRLILLPIIGGTAVTISFLSTFIGKMLATFLYPINNVVLSHISVNESDNIKKQYLKT
                     NLFAIAAL"
BASE COUNT         4072 a         1503 c         2076 g         3561 t
ORIGIN      
        1 aataaagaaa atattaagat tgatactcat ttcacaaact attactactt tagagtataa
       61 ttatttttaa tttcatataa atgaatatgc gtaaataatg cgatttaaaa gtaattaatt
      121 gtttaaatga tatgtaatat gtaaatacta tatatataat accaatttta atgaaaattt
      181 ttaagggagg taaataatgg aaagtacatt agaattaaca aaaattaaag aagtattaca
      241 aaaaaacttg aaaattttaa ttattttacc gctattattt ttaataatta gcgctattgt
      301 tacatttttc gtcttatcac ctaaatatca agctaatact caaatcttag tgaatcaaac
      361 taagggtgac aatcctcagt ttatggcaca agaggttcaa agtaatattc aacttgtaaa
      421 tacgtataaa gaaattgtta aaagtcctag aattttagat gaagtgtcaa aggacttaaa
      481 tgataagtat tcaccatcta aattgtcgag catgttgaca attacgaatc aagaaaatac
      541 acaacttatc aacatccaag ttaaaagtgg tcataaggaa gattcggaaa aaattgcgaa
      601 tagctttgcc aaagtgacaa gtaaacaaat tccgaagatt atgagtgtag ataacgtatc
      661 aattttatct aaagcagacg gtacagcagt taaagtcgca ccaaaaactg tagtgaatct
      721 aatcggtgca ttctttttag gattagttgt cgcgcttata tatatcttct tcaaagtaat
      781 tttcgataag cgaattaaag atgaagaaga tgtagagaaa gaattaggat tgcctgtatt
      841 gggttcaatt caaaaattta attaaggatg gttgctactt atgtcaaaaa aggaaaatac
      901 gacaacaaca ctatttgtgt atgaaaaacc aaaatcaaca attagtgaaa agtttcgggg
      961 tatacgttca aacatcatgt tttcaaaagc aaatggcgaa gtaaagcgct tattagttac
     1021 ttctgaaaaa cctggtgcag gtaaaagtac agtcgtatcc aatgtagcga ttacgtatgc
     1081 acaagcaggt tataaaacgt taattatcga tggggatatg cgtaagccaa cacaaaacta
     1141 tatttttaat gaacaaaata ataatggatt atcgaattta atcattggta gaacgactat
     1201 gtcagaagct attacgacga cagaaattga taatttagat ttactaacag ctggtcctgt
     1261 acctcctaat ccaacagaat taattggttc tgaaagattt aaagagttag ttgatctttt
     1321 caataaacgt tacgacatta ttattgtcga tacaccgcca gttaataccg tgactgatgc
     1381 acaactatat gcgcgtgcta ttaaagatag tctgttagtc attgatagtg aaaaaaatga
     1441 taaaaatgaa gttaaaaaag ctaaaatact tatggaaaaa gcaggcagta acattctggg
     1501 cgtcatttta aataaggcaa aagtagataa atcttctagt tattatcact attatggaga
     1561 tgaataagta tgattgatat tcataatcat atattgccta atatcgatga cggtccgaca
     1621 aatgaaacag agatgctaga tcttttaaaa caagcgacaa cacaaggtgt tacagaaatc
     1681 attgtaacat cacatcactt acatcctcga tataccacac ctatagaaaa agtgaaatca
     1741 tgtttaaacc atattgaaag cttagaggaa gtacaagcac tgaatctaaa gttttattat
     1801 ggtcaggaaa taagaattac cgatcaaatc cttaatgata ttgatcgaaa agttattacc
     1861 ggtattaatg attcacgcta tttactaata gaatttccat caaatgaagt tccacactat
     1921 actgatcaat tatttttcga attacaaagt aaaggctttg taccgattat tgcacatcca
     1981 gagcggaata aagcaataag tcaaaacctt gacatactat acgatttaat taacaaaggt
     2041 gctttaagtc aagtgacaac ggcgtcatta gcgggtattt ccggtaaaaa aattagaaaa
     2101 ttagcaattc aaatgattga aaacaatctg acacatttca tcggttcaga tgcgcataac
     2161 acagaaatca gaccgttctt aatgaaagac ttatttaatg ataagaaatt acgtgattat
     2221 tatgaagata tgaacggatt tattagtaat gcgaagttag ttgttgatga taaaaaaatt
     2281 cctaaacgaa tgccacaaca agattataaa cagaaaagat ggtttgggtt ataaacagca
     2341 aatgaggggt tttatggcac atttatctgt gaaattgcgg cttttaatac tagcattaat
     2401 cgattcactg atagtgacat tttcagtatt cgtaagttat tacattttag aaccgtattt
     2461 caaaacatat tctgtcaaat tattaatatt ggcagctata tcactattca tatcgcatca
     2521 tatttcagca tttattttta atatgtatca tcgagcgtgg gaatatgcca gtgtgagtga
     2581 attgatttta attgttaaag ctgtgacgac atctatcgtt attacgatgg tggtcgtgac
     2641 aattgttaca ggcaatagac cgttttttag attgtattta attacttgga tgatgcactt
     2701 gattttaata ggtggctcaa ggttattttg gcgtatttat cggaaatacc ttggaggtaa
     2761 gtcatttaat aagaagccaa ctttagttgt tggtgctggc caagcaggtt caatgctgat
     2821 tagacaaatg ttgaaaagtg acgaaatgaa acttgaaccg gtattagcag tcgatgatga
     2881 cgaacataaa cgcaatatca caattactga gggtgtaaaa gtccaaggtg aaattgcgga
     2941 tattccagaa ctagtgagga aatataagat taaaaaaatc atcattgcaa ttccaactat
     3001 tggtcaagag cgtttgaaag aaattaataa tatttgccat atggatggcg ttgagttatt
     3061 gaaaatgcca aatatagaag acgtcatgtc tggtgagtta gaagtgaacc aacttaaaaa
     3121 agttgaagta gaagatttac taggcagaga tcctgttgaa ttagatatgg atatgatatc
     3181 aaatgaattg acgaataaaa ctattttagt tacgggtgca ggtggttcaa taggatcaga
     3241 aatttgtaga caagtttgta atttctatcc agaacgtatt attctacttg gccatggtga
     3301 aaacagtatt tatttaatca atcgtgaatt gcgaaatcgc ttcggaaaaa atgttgatat
     3361 cgttcctatt atagcggatg tgcaaaatag agcgcgtatg tttgaaattt tggaaatgta
     3421 taaaccatac gcagtttatc atgcagcagc acacaagcac gtgccgttaa tggaagacaa
     3481 ccctgaagaa gcagtacgta ataatatttt aggtacgaaa aatactgctg aagctgctaa
     3541 aaatgcagag gtaaagaaat tcgttatgat ttcgacggat aaagccgtta atccgcctaa
     3601 tgtcatggga gcttcaaagc gaattgcaga aatgattatt caaagtttaa atgatgaaac
     3661 acatcaaaca aattttgttg cagtgagatt tggtaatgta cttggatcga gagggtctgt
     3721 gattccactt ttcaaaagtc aaattgaaga aggtgggcca gttactgtga cacatcctga
     3781 aatgacacgt tactttatga caattcctga agcttctaga ctagttttgc aggcaggggc
     3841 attagcagaa ggtggcgaag tatttgtgct agatatggga gaaccagtga aaattgtaga
     3901 tttggcacgt aatttaatta agctaagtgg taaaaaagaa gacgacatac gcattactta
     3961 tacagggatt agatccggcg aaaaaatgtt tgaagagctt atgaataaag atgaagttca
     4021 tcctgaacaa gtatttgaaa aaatttatcg tggcaaagta caacatatga aatgtaatga
     4081 agttgaagcc attattcaag acatcgtcaa tgactttagt aaagaaaaaa ttattaacta
     4141 tgccaatggc aaaaagggag ataattatgt tcgatgacaa aattttatta attactgggg
     4201 gcacaggatc attcggtaat gctgttatga aacggttttt agattctaat attaaagaaa
     4261 ttcgtatttt ttcacgcgat gagaaaaaac aagatgacat tcgaaaaaaa tataataatt
     4321 caaaattaaa gttctacatt ggtgatgtgc gtgatagtca aagtgtagaa acagcaatgc
     4381 gagatgttga ttacgtattc catgcagcag ctttaaaaca agtgccgtca tgtgaattct
     4441 ttccagttga ggcagtgaag acaaatatta ttggtacaga aaatgtctta caaagtgcta
     4501 ttcatcaaaa tgttaaaaaa gtcatatgtt tatctacaga taaggcagcg tatcctatta
     4561 atgctatggg tatttcgaaa gcaatgatgg aaaaagtatt catagccaaa tcaagaaata
     4621 ttcgtagtga acaaacgctt atttgtggta caagatacgg taatgtgatg gcttcaagag
     4681 gatcagtaat tcctttgttt atcgacaaaa tcaaagctgg agaaccttta acgattacag
     4741 atcctgatat gacaagattt ttaatgagtt tagaagatgc cgtggaacta gttgttcatg
     4801 catttaagca tgcagagaca ggcgatatca tggttcaaaa agcaccaagc tcaacagtag
     4861 gggatcttgc gaccgcatta ttagaattgt ttgaagctga taatgcaatt gaaatcattg
     4921 gtacgcgaca tggagagaaa aaagcagaaa cattgttgac gagagaagaa tacgcacaat
     4981 gtgaagatat gggtgattat tttagagtgc cggcagactc cagagattta aattatagta
     5041 attatgttga aaccggtaac gaaaagatta cgcaatctta tgaatataac tccgataata
     5101 cacatatttt aacggtggaa gagataaaag aaaaactttt aacactagaa tatgttagaa
     5161 acgaattgaa cgattataaa gcttcaatga gataggagag attgacgttg aatattgtaa
     5221 ttacaggagc aaagggtttt gtaggaaaaa acttgaaagc agatttaaca tcaacgacag
     5281 attatcatat tttcgaagta catcgacaaa ctaaagagga agaattagag tcagcgttgt
     5341 tgaaagcaga ctttgtcgtg catttagcag gtgttaatcg acctgaacac gacaaagaat
     5401 tcagcttagg aaacgtgagt tatttagatc atgtacttga tatattaact agaaataaga
     5461 aaaagccggc gatattatta tcgtcttcaa tacaagcaac acaagataat ccttatggtg
     5521 agagtaagtt gcaaggggaa cagctattaa gagagtattc cgaagagtat ggcaatacgg
     5581 tttatattta tcgatggcca aatttattcg gtaagtggtg taagccgaat tataactcag
     5641 tgattgcaac attttgttac aaaattgcac gtaacgaaga gattcaagtt aatgatcgga
     5701 atgttgaact gacgctaaac tacgtggatg atatcgtcgc tgaaataaaa cgtgctattg
     5761 aaggaactcc aacgattgaa aatggtgtac ctacagtacc aaacgtattt aaagtgacat
     5821 tgggagaaat tgtagattta ttatacaagt tcaaacagtc acgtctcgat cgaacattgc
     5881 cgaaattaga taacttgttt gaaaaagatt tgtatagtac gtatttaagc tatctaccta
     5941 gtacagactt tagttatccc ttacttatga atgtggatga taggggttct tttacagaat
     6001 ttataaaaac accggatcgt ggtcaagttt ctgtaaatat ttctaaacca ggtattacta
     6061 aaggtaatca ctggcatcat actaaaaacg aaaaatttct agtcgtatca ggtaaagggg
     6121 taattcgttt tagacatgtt aatgatgatg aaatcattga atattacgtt tctggcgaca
     6181 aattagaagt tgtagacata ccagtaggat acacacataa tattgaaaat ttaggcgaca
     6241 cagatatggt aactattatg tgggtgaatg aaatgtttga tccaaatcag ccagatacgt
     6301 atttcttgga ggtatagcgc atggaaaaac tgaaattaat gacaatagtt ggtacaaggc
     6361 ctgaaatcat tcgtttatca tcaacgatta aagcatgtga tcaatatttt aatcagatat
     6421 tagtacacac tggtcaaaat tatgattata cattgaatca aattttcttt gatgatttgg
     6481 aattaagaca accggaccac tacttagagg cagttggaag taaccttgga gaaacgatgg
     6541 ggaatattat tgcgaagaca tatgacgttt tattacgcga acaaccagat gcacttttaa
     6601 ttcttggtga tacaaatagt tgtttagcag cagtatctgc taaacgatta aaaattcctg
     6661 tattccacat ggaagcgggt aatagatgct ttgatcagaa tgtacctgaa gaaatcaatc
     6721 gtaaaattgt tgaccatgtc agtgatgtga atctacctta tacggaacat agcagacgtt
     6781 atttattaga tgaaggcttc aataaagcga atatctttgt gacaggatca ccgatgacag
     6841 aagtgataga agcgcatcga gataaaatta atcacagtga cgttttaaat aaactaggat
     6901 tagaaccgca acaatacatt ttagtatctg cgcatagaga agagaatatc gataatgaaa
     6961 agaattttaa atcattaatg aatgcgataa atgatattgc caaaaagtat aaaatgcctg
     7021 tgatttattc aacgcatcca agaagttgga agaaaattga agaaagtaaa tttgaatttg
     7081 atccactagt taaacagtta aagccatttg gtttctttga ttataatgca ttgcaaaaag
     7141 atgcatttgt tgtgctatca gatagtggaa cattgtcaga agagtcgtct attttgaagt
     7201 tccctggtgt ccttattcga acttccacag aaagaccgga agtactagat aaaggtacgg
     7261 ttattgtagg tggtattacc tataacaatc taatccaatc cgttgaacta gcaagagaga
     7321 tgcaaaacaa taacgaaccg atgattgatg ctattgatta taaagacact aatgtttcga
     7381 caaaggtagt taaaattatt caaagctata aagatattat caatcgaaat acttggagga
     7441 aatgacgatg aggatagcga ttgaaaagat aattggtttg ctgaaaaacc agtcctctaa
     7501 agaatcgaat gttaagattc atcgcttggc gtatattaca aactcaaaat ttgatggcaa
     7561 taactatata gatagatggt gtaaaatcag gaattctcac attggtgaat acagttatat
     7621 tggatttggt agtgatttta ataatgtaga agtaggaaga tattgttcga tatcttcgga
     7681 tgtaaaaatt gggttaggaa aacatcctac acactttttt agctcatcac cgatttttta
     7741 ttctaataat aatccattta acataaagca aaagtttata gactttaatg accaaccaag
     7801 ccgtacaaca attaaaaatg atgtgtggat tggtgcaaat gtaattatta tggatggatt
     7861 aacaataaat actggtgcag tcatagcagc cggctcagtt gttactaaaa atgtaggagc
     7921 atatgaggtt gttggtgggg ttcctgcaaa agtgattaag aagcgatttg acaataaaac
     7981 aattgaaaaa cttttggaaa gcaagtggtg ggagaaaacg cctgacaaac taaaaggatt
     8041 ttcggttgaa tatttaaata aaaaggatac ttaatgatat gagaatttta aatattgtat
     8101 cgagtaatat tgttcaagac ccaagggtac ttaaacaaat agaaacaatt aaaggcgtta
     8161 cgaatgatta taaaattgtt ggaatgaata attcacaagc tactaataag cgattggaaa
     8221 atttagattg taattatcgt ttgttaggta gcaaggtaga tccaaaaaat attctttcta
     8281 aattaattaa gcgtataaga tttgcaacag gtgttatccg agaaattaaa gcttataaac
     8341 ctgacgtgat acatgcaaat gatttcgacg tattattaat ggtctattta agcaattata
     8401 aaaaagctaa tattgtttat gatgcgcatg aaatatatgc gaaaaatgcc tttattaata
     8461 aagttccact tatttcaaag tttgtagaaa gtatagaaaa acacattgta aaacatcgtg
     8521 ttaatgcctt cgtaacagta agtcatgcag caaaagaata ttatcaatct aaaggatata
     8581 agaaggaagc gaatgttatt acgaatgcac ctattttaaa tgatagcaga gaatttaaag
     8641 aaatcgaaaa ctttaaatca atcgtatatc aaggtcaaat tgtaatggac agaggatatg
     8701 aagagtttat tatagcttca tcagctttta aacaaaatgc cccttcattc ataattcgag
     8761 ggtttggtcc gcatgaagaa gtgataaaag aactgattag ttataacccg gaaaatatta
     8821 ggttggataa accagttgaa gtaaaagaat tggttgataa gttagcagaa agtaatgttg
     8881 gtgttgtctt gacgaaacct gtatctatta attttgaata tacagtatct aataaaattt
     8941 ttgaatgtat acatgctggt ttaccagtaa ttttatctcc tgtcaaagag catatttatc
     9001 tcaatgaaaa atataaattt ggcattgttc taaaggaagt tacgccgtta gaaattgaaa
     9061 aggcggttag aaaattaaga gataatcacg atttgtttaa tcatttacgt caaaatgcaa
     9121 ttaaggcgtc taaaattttg aattggcaaa tagaaagtga acgattagta aaattatata
     9181 aattttaaag agaggtaaac tatgaaattt tttgtacttt gtgcaattat cagcatgaac
     9241 atatttatag taatctctac atttactaaa gaagtattag ggttccctat agagccggtg
     9301 tattactcaa ccatggttgg tatagcatta attactacgg tgtttgctat ttataagata
     9361 attgtcacgc aagaaattcc gcgagggtta atattattaa ttgctatatg tttgctttat
     9421 ctagcttttt attatttttc accagataag gaagagaaac tagctaaaaa taatattcta
     9481 ttctttttaa catgggcagt tccagcggca attagtggta tttatattaa atatataaac
     9541 aaggctacgg tagaaagatt ttttaaatta gtatttttca tattttctgt ttcatttatt
     9601 tttgtaattt taataccaaa acttacaggt gagataccta gctatatcaa ttttggactt
     9661 atgaactatc aaaacgcttc gtacctttca gcatttactg ccggattagg catttatttc
     9721 attatgaaag gttcagtgaa acataagtgg atatatgttc tatttacaat aattgatatc
     9781 cctattgtgt ttataccagg agggcgtgga ggtgctattt tattaattct ttacggctta
     9841 tttgcattta tacttattac gtttaaaaga ggaataccta ttgcagtaaa aagcattatg
     9901 tatatttctg cattaagcat atctagtgta ttgatttact ttctttttac aaaaggttcg
     9961 aatactagaa cattttcata tctacaaggt ggaacactta atttagaagg tacttctgga
    10021 agaggaccga tttatgaaaa aggtatttac tttattcaac aaagtccgtt attaggctat
    10081 gggccattta actattataa actaatcgga aatataccac ataacatcat tattgagttg
    10141 attctatcat ttggcttatt agggtttttt atcataatga tttgcatttt gctactagtt
    10201 tataaaatga ttaggaacta tgatccaaac actatagatt tactcgttat gtttatagca
    10261 atctatccaa tcacattatt aatgtttagt tcaaattatt tagttgtaag tgaattttgg
    10321 tttgtgttgt tctattttat tacaaaagga cggcgtcatc atggttaaga aagtttttat
    10381 tatggatagt gtaaagacaa taattggtac gttgcttata gctttaggat tacaattttt
    10441 agcttatcca attattaatc aacgagtagg taatgaagcg tttggttcta ttttaacgat
    10501 ttatacaata ataacaatca cgagtgttgt attaggcaat acgcttaaca atatacgatt
    10561 gattaatatg aatctataca aatccaatca ttactactgg aaatttgtgt tgatactttt
    10621 aatttcaatt ctgattgaga gtatagcttt aattattgta tttctttact tttttaattt
    10681 gaacaccatc gatattatct ttttaattct acttaatatt ttaatgtgct taaggattta
    10741 tctgaatgta ttttttagga tgactttaaa atataatcag attttgtata ttgctcttat
    10801 tcaattttta ggtttgttga taggactact tctatattat ttaacccaaa actggattgt
    10861 ttgttttatt accagtgaat tgtttgcaac gatatataca ttggttaaat tacggggatt
    10921 aactataggc gcgtatcaaa gtgaagataa taatgtggta aaagattatg tgatgctact
    10981 gagtacaaat agccttaata atttgaatct ctacttagat agattaatct tattaccaat
    11041 tataggtgga acagctgtaa ctatatcatt tctttcaaca tttattggga aaatgttagc
    11101 tacatttctg tatccgatta ataatgtagt gctttcacat atttctgtaa atgaaagtga
    11161 caatataaag aaacaatatt tgaaaactaa tctatttgct atagctgccc ta
//
LOCUS       DAHHOJ010000085        11190 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.085, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000085 DAHHOJ010000000
VERSION     DAHHOJ010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11190
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.085"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..746
                     /gene="modA"
                     /locus_tag="NBH32_001954"
     CDS             <1..746
                     /gene="modA"
                     /locus_tag="NBH32_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCD1515536.1"
                     /translation="FLVLAGCSNSNDNQDNKKDATDNGKKQEIQVAAAASLTDVTKKL
                     ASEFKKEHKNADIKFNYGGSGALRKQIESGAPVDVFMSANTKDVDALKDKNKAHNTYK
                     YAKNSLVLIGDKDSNYTSVKDLKGNDKLALGEVKTVPAGKYAKQYLDNNNLFKEVESK
                     IVYAKDVKQVLNYVEKGNAKQGFVYKTDLYKQNKKIDTVKVIKEVELKKPITYEAGAT
                     SDSKLAKEWMEFLKSDKAKEILKEYHFAA"
     gene            760..1431
                     /gene="modB"
                     /locus_tag="NBH32_001955"
     CDS             760..1431
                     /gene="modB"
                     /locus_tag="NBH32_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCD1515537.1"
                     /translation="MPDLTPFWISIRVAVISTIIVTVLGIFISKWLYRRKGSWVKVLE
                     SLLILPIVLPPTVLGFILLIIFSPRGPIGQFFANVLHLPVVFTLTGAVIASVIVSFPL
                     MYQHTVQGFRGIDTKMINTARTMGASETKIFLKLILPLAKRSILAGIMMSFARALGEF
                     GATLMVAGYIPNKTNTLPLEIYFLVEQGRENEAWLWVLVLVAFSIVVISTINLLNKDK
                     YKEVD"
     gene            1432..2037
                     /locus_tag="NBH32_001956"
     CDS             1432..2037
                     /locus_tag="NBH32_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1515538.1"
                     /translation="MLKINVKYQLKNTLIRINIDDTEPKIYAVRGPSGIGKTTVLNMI
                     AGLRKADEAIIEVNGQLLTDTAKNVNVKIQQRRIGYLFQDYQLFPNMTVYKNITFMAE
                     PSEHIDQLIQTLNIDHLMKQYPMTLSGGEAQRVALARALSTKPDLILLDEPFSSLDDT
                     TKDESITLVKRIFNEWQIPIIFVTHSNYEAEQMAHEIITIG"
     gene            2206..3210
                     /locus_tag="NBH32_001957"
     CDS             2206..3210
                     /locus_tag="NBH32_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HCD1515539.1"
                     /translation="MSQERYSRQILFKQIGEIGQSKINQKCALIIGMGALGTHVAEGL
                     VRAGIAKLIIVDRDYIEFSNLQRQTLFTEEDALKMMPKVVAAKKHLLALRSDVDIDDY
                     IAHVDYYFLETHGQDVDVIIDATDNFETRQLINDFAYKHRIPWIYGGVVQSTYTEAAF
                     IPGKTPCFNCLVPQLPALNLTCDTVGVIQPAVTMATSLQLRDAMKVLTEQPIDTKITY
                     GDIWEGSHYSFGFSKMQRSDCTTCGDVPSYPYLNKNEQRYATLCGRDTVQYENASITH
                     DILVQFLKQHQLNYRSNSYMVMFEFKGHRIVAFKGGRFLIHGMTRTSDATHLMNLLFG
                     "
     gene            3241..3747
                     /locus_tag="NBH32_001958"
     CDS             3241..3747
                     /locus_tag="NBH32_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaB"
                     /protein_id="HCD1515540.1"
                     /translation="MGEHQNVKLNRTVKAAVLTVSDTRDIDTDKGGQCVRQLLQADDV
                     EVSDAHYTIVKDEKVAITTQVKKWLEEDIDVIITTGGTGIAQRDVTIEAVKPLLTKEI
                     EGFGELFRYLSYVEDVGTRALLSRAVAGTVNNKLIFSIPGSTGAVKLALEKLIKPELN
                     HLIHELTK"
     gene            complement(3749..4243)
                     /gene="moaC"
                     /locus_tag="NBH32_001959"
     CDS             complement(3749..4243)
                     /gene="moaC"
                     /locus_tag="NBH32_001959"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCD1515541.1"
                     /translation="MTEFTHINQQGHAKMVDVSDKQITKRTAVAHSSITVNETIFKQI
                     SNNTNTKGNVLNTAQIAGIMAAKNTSTIIPMCHPLPLTGIDVHFSWDETNAPLYTLNI
                     QTTVSTTGKTGVEMEALTAASATALTIYDMTKAVDKGMIIGETYLESKSGGKSGDFQR
                     QSNQ"
     gene            4306..5565
                     /locus_tag="NBH32_001960"
     CDS             4306..5565
                     /locus_tag="NBH32_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCD1515542.1"
                     /translation="MVVEKRNPIPVKEAIQRIVNQQSSMPAITVALEKSLNHILAEDI
                     VATYDIPRFDKSPYDGFAIRSVDSQGASGQNRIEFKVIDHIGAGSVSDKLVGDHEAVR
                     IMTGAQIPNGADAVVMFEQTIELEDTFTIRKPFSKNENISLKGEETTTGDVVLKKGQV
                     INPGAIAVLATYGYAEVKVIKQPSVAVIATGSELLDVNDVLEDGKIRNSNGPMIRALA
                     EKLGLEVGIYKTQQDDLDSGIQVVKEAMEKHDIVITTGGVSVGDFDYLPEIYKAVKAE
                     VLFNKVAMRPGSVTTVAFADGKYLFGLSGNPSACFTGFELFVKPAVKHMCGALEVFPQ
                     IIKATLMEDFTKANPFTRFIRAKATLTSAGATVVPSGFNKSGAVVAIAHANCMVMLPG
                     GSRGFKAGHTVDIILTESDAAEEELLL"
     gene            5562..6047
                     /gene="mobB"
                     /locus_tag="NBH32_001961"
     CDS             5562..6047
                     /gene="mobB"
                     /locus_tag="NBH32_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCD1515543.1"
                     /translation="MILQIVGYKKSGKTTLMRHIVSFLKSHGYTVATIKHHGHGKKDI
                     QLQDSDVDHMKHFEAGADQSIVQGFQYQQTVTRVDNQNLTQIIEKSVTIDTNIVLVEG
                     FKNADFEKVIVYRNEEELQVLQQLSNVCYSINVREHEDFTAFEQWLLNKIKNDCDTQL
                     T"
     gene            6061..6507
                     /locus_tag="NBH32_001962"
     CDS             6061..6507
                     /locus_tag="NBH32_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HCD1515544.1"
                     /translation="MKQFEIVTEPIQTEQYREFTINEYQGAVVVFTGHVREWTKGVKT
                     EYLEYEAYIPMAEKKLAQIGDEINEKWPGTITSIVHRIGPLQISDIAVLIAISSPHRK
                     DAYRANEYAIERIKEIVPIWKKEIWEDGSKWQGHQKGNYEEAKREE"
     gene            6513..6746
                     /gene="moaD"
                     /locus_tag="NBH32_001963"
     CDS             6513..6746
                     /gene="moaD"
                     /locus_tag="NBH32_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HCD1515545.1"
                     /translation="MKVLYFAEIKDILQKAQEDIVLEQALTVQQFEDLLFERYPQINN
                     KKFQVAVNEEFVQKSDFIQPNDTVALIPPVSGG"
     gene            6753..7352
                     /gene="mobA"
                     /locus_tag="NBH32_001964"
     CDS             6753..7352
                     /gene="mobA"
                     /locus_tag="NBH32_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCD1515546.1"
                     /translation="MKAIILAGGHSVRFGKPKAFAEVNGETFYSRVIKTLESTNMFNE
                     IIISTNAQLATQFQYPNVVIDDENHNDKGPLAGIYTIMKQHPEEELFFVVSVDTPMIT
                     GKAVSTLYQFLVSHLIENHLDVAAFKEDGRFIPTIAFYSPNALGAITKALHSDNYSFK
                     NVYHELSTDYLDVRDVDAPSYWYKNINYQHDLDALIQKL"
     gene            7372..8394
                     /gene="moaA"
                     /locus_tag="NBH32_001965"
     CDS             7372..8394
                     /gene="moaA"
                     /locus_tag="NBH32_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCD1515547.1"
                     /translation="MVEQIKDKLGRPIRDLRLSVTDRCNFRCDYCMPKEVFGDDFVFL
                     PKNELLTFDEMARIAKVYAELGVKKIRITGGEPLMRRDLDVLIAKLNQIDGIEDIGLT
                     TNGLLLKKHGQKLYDAGLRRINVSLDAIDDTLFQSINNRNIKATTILEQIDYATSIGL
                     NVKVNVVIQKGINDDQIIPMLEYFKDKHIEIRFIEFMDVGNDNGWDFSKVVTKDEMLT
                     MIEQHFEIDRVEPKYFGEVAKYYRHKDNGVQFGLITSVSQSFCSTCTRARLSSDGKFY
                     GCLFATVDGFNVKAFIRSGVTDEELKEQFKALWQIRDDRYSDERTAQTVDNRQRKKIN
                     MNYIGG"
     gene            complement(8679..8849)
                     /locus_tag="NBH32_001966"
     CDS             complement(8679..8849)
                     /locus_tag="NBH32_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515548.1"
                     /translation="MFRFIYFGQLVVYSTTFIPKKDILQLNDMGCDINVKEPLKIYYV
                     VILKSGIEMIFS"
     gene            8975..9325
                     /gene="sarV"
                     /locus_tag="NBH32_001967"
     CDS             8975..9325
                     /gene="sarV"
                     /locus_tag="NBH32_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarV"
                     /protein_id="HCD1515549.1"
                     /translation="MSNKVQRFIEAERELSQLKHWLKTTHKISIEEFVVLFKVYEAEK
                     ISGKELRDTLHFEMLWDTSKIDLIIRKIYKKELISKLRSETDERQVFYFYSTSQKKLL
                     DKITKEIEVLSVTN"
     gene            complement(9427..10638)
                     /locus_tag="NBH32_001968"
     CDS             complement(9427..10638)
                     /locus_tag="NBH32_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515550.1"
                     /translation="MTNQGSLNKSPIFTKSFTINFFVNFIVYLCMYLLLVVIAGYSKQ
                     AFDASDSLAGLVVGLFIVGSLIGRFATGKFVNQIGPKRLLFIGLIALIITQLLYFIDG
                     SLAFLIFVRLINGIATAVVTTATGTIAAYVTPVNRKSEGISLFSLSLVLGTAIGPFLG
                     MLLITKYAIDLLFIICVILGILGLIISLFIKVDFEVTNNKTETNVIDKPRFSIHQFIA
                     KEAIPVAVIMLLIGVTYASILTYLQAFALERHLVTAASYFFICYAIASLITRPIAGRL
                     MDDKNENIIVYPAFIMLFLSFVCLISSYQSWLILLAGACLGLGYGNLSSAMQSIAIKV
                     SPPIKYGIATSTFYVGLDAGVGFGPSFLGLFTHMFSYSEIFGFMAALAIITMLVYFLI
                     HGRHVTRNAMN"
     gene            complement(10631..11071)
                     /locus_tag="NBH32_001969"
     CDS             complement(10631..11071)
                     /locus_tag="NBH32_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HCD1515551.1"
                     /translation="MLSQEFFNSFITIYRPYLKLAEPILEKHNIYYGQWLILRDIAKH
                     QPTTLIEISHRRAIEKPTARKTLKALIENDLITVENSLEDKRQKFLTLTPKGHELYEI
                     VCLDVQKLQQAVVAKTNISQDQMQETINVMNQIHEILLKEAHND"
BASE COUNT         4005 a         1595 c         2158 g         3432 t
ORIGIN      
        1 tatttttagt attagctggt tgttctaatt ctaatgataa tcaagacaac aaaaaagatg
       61 caacagacaa tggaaaaaaa caagaaattc aagttgcagc ggcagcaagt ttaacagatg
      121 taaccaagaa attagcttca gaatttaaaa aagagcataa aaatgctgat attaaattta
      181 actatggtgg atcaggggca ttaagaaaac aaattgaatc aggcgcacct gttgacgtat
      241 ttatgtctgc aaatactaaa gatgtagatg cattaaaaga caagaataaa gcacataata
      301 catataaata tgcgaaaaat agtctagtac taattggtga taaagattca aattacactt
      361 cagtaaaaga cttaaaaggc aatgataaat tagcattagg tgaagtgaaa actgtaccag
      421 caggaaaata tgcgaaacag tatttagata acaataactt atttaaagaa gttgaaagta
      481 aaatcgttta tgctaaagat gtaaaacaag tattaaatta tgttgaaaag ggtaatgcga
      541 aacaaggttt tgtatataaa actgacttat ataaacaaaa caaaaaaatt gatactgtaa
      601 aagtaattaa agaagtagaa cttaagaaac caatcacata cgaagctggt gctacatcgg
      661 atagtaaatt agcaaaagag tggatggaat tcttaaaatc agataaagct aaagaaatat
      721 taaaagaata ccactttgca gcataaggag ttgtaatcca tgcctgactt aacacctttt
      781 tggatatcca tacgagttgc tgtaatcagt acgattattg taacggtttt aggtattttt
      841 atatctaaat ggttgtatcg tcgtaagggt tcgtgggtta aagtattgga aagtttattg
      901 atattaccta ttgttttgcc gccaacggta ttaggtttta ttctattaat catcttctcg
      961 ccaagaggac caatcggtca attctttgcg aatgtactac atttacctgt agtgttcact
     1021 ttgacaggtg ctgtgatagc atctgtcatt gttagttttc cactaatgta tcaacatact
     1081 gtgcaaggct tcagaggtat agacacgaaa atgattaata cagctagaac aatgggagca
     1141 agtgaaacga aaattttcct caaattaatt ttaccattag ctaaacgctc tattttagca
     1201 ggtataatga tgagttttgc tcgtgcatta ggtgagtttg gtgctacatt aatggttgca
     1261 ggatatattc caaataaaac gaatacacta cctttagaaa tatacttttt agtggaacaa
     1321 ggtagagaaa atgaagcgtg gttatgggta ttagtgctag tcgcattctc tattgtggtt
     1381 atatctacaa ttaatttatt gaataaagat aaatataagg aggtcgacta gatgcttaaa
     1441 atcaatgtga aatatcaatt aaagaacact ttaattcgca tcaatataga tgatactgaa
     1501 ccaaaaattt atgcagttcg tggtccgtct ggcattggta aaactactgt tttaaatatg
     1561 attgccggat tacgtaaagc agatgaagct attatcgaag tgaatgggca attgcttact
     1621 gatacggcaa aaaacgtgaa tgttaaaatt caacaacgac gtattggata tctgtttcaa
     1681 gactaccaat tgtttcctaa tatgacggtc tataaaaata ttacttttat ggctgaacca
     1741 tctgaacaca tcgatcaatt aatccaaact ttaaacattg atcatttgat gaaacaatat
     1801 cctatgacat tgtcaggtgg agaggcacaa cgtgtagcac ttgcacgtgc acttagcacg
     1861 aaaccagatt taattttatt agatgaacct ttttctagtt tggatgatac tacaaaagat
     1921 gagagtatta cattagttaa acgtattttc aacgaatggc aaataccaat catatttgtg
     1981 acacattcaa actatgaagc agaacaaatg gctcatgaaa ttattacaat tgggtaatca
     2041 tttatttgcc attaaagagt ttagaacgta tttaaaattg tagaagtgaa tgcttctatc
     2101 agcattttaa tgatgtttta aactcttttt taggggcagt tttttgagag acattgacgc
     2161 gcgtcatata atgaaagtaa tgataaaaag aaaggataac ttaatgtgag tcaagaacgt
     2221 tattcaaggc aaattttatt taaacaaata ggtgaaatag gtcaaagcaa aataaatcaa
     2281 aaatgtgcgt tgattattgg tatgggcgca ttaggtacac atgtggccga aggacttgtt
     2341 agagcaggca ttgctaaact aatcattgtt gatagagatt atattgaatt tagtaattta
     2401 caaagacaaa cattgtttac tgaagaagat gctttgaaaa tgatgcctaa agtggttgca
     2461 gctaaaaagc atttgctagc gttacgtagt gatgttgata ttgatgatta tattgcccat
     2521 gtggattatt attttttgga aacacatgga caggacgttg acgttattat tgatgcaacc
     2581 gataactttg aaacacgaca actgattaat gattttgcat ataaacatcg tataccttgg
     2641 atttatggtg gtgttgtaca gagtacatat acagaagctg catttatacc tggtaaaaca
     2701 ccttgcttta actgtttggt accacaattg ccagcattaa atttaacatg tgatacagta
     2761 ggggtcattc aacctgccgt gacgatggca acaagtttac aattaagaga tgcgatgaaa
     2821 gtattaacgg aacaaccaat tgacacaaaa ataacttatg gcgatatttg ggaaggtagt
     2881 cattattcat ttggtttcag taaaatgcaa cgttcagact gtacaacttg tggagatgta
     2941 ccaagttatc cgtatttaaa caagaatgaa caacgttatg caacattgtg tggtagagac
     3001 actgtacagt atgaaaatgc atcaattaca cacgacattc ttgttcaatt tttaaaacaa
     3061 catcaactaa attatcgcag taattcgtat atggttatgt ttgaatttaa aggacaccgc
     3121 attgttgctt ttaaaggtgg aaggttttta atacatggca tgacacgcac atcagatgcc
     3181 acacatctaa tgaatttatt gtttggataa aaaaagataa gacaaaagga gtgtaatatt
     3241 atgggcgaac atcaaaacgt taaattgaat cgtacagtta aagcagccgt actaacggta
     3301 tcagatacta gagacattga tacagataaa ggtggtcaat gcgtgcgcca actattacaa
     3361 gcagatgacg ttgaagtgag tgacgcacat tatacaattg tgaaagatga aaaagtagcc
     3421 atcacgacgc aggtaaagaa gtggttagaa gaagatattg atgtcatcat tacgactggt
     3481 ggaacaggta ttgcacaacg tgatgtgacg attgaagcag taaaaccact tttaactaaa
     3541 gagatagaag gctttgggga attgtttaga tatttgagtt atgttgaaga tgttggcacg
     3601 cgtgcattat tgtctcgtgc tgtagcaggt acagttaata ataaattgat attttcgatt
     3661 ccaggatcaa caggcgcagt taaattagca ttagaaaagc tcattaaacc agaattaaat
     3721 catctgattc atgagcttac aaaataattt attgatttga ttggcgttga aaatctccag
     3781 atttaccgcc agacttgctt tcaaggtagg tttcgccaat aatcatacct ttatcaactg
     3841 ctttcgtcat gtcgtaaatg gttaaagccg ttgctgatgc agcggttaaa gcttccattt
     3901 caacaccggt tttgccagtt gtagagacag ttgtttgaat gtttaaagta taaaggggtg
     3961 catttgtttc atcccagctg aagtgaacat ctatgccagt caatggcaat ggatggcaca
     4021 tcggaataat tgttgatgta tttttagcag ccataatacc agcgatttga gcagtgttca
     4081 atacattgcc tttggtattg gtgttattag aaatctgctt aaaaatagtt tcattcacag
     4141 taatgcttga atgagcgaca gcagttcttt ttgtaatttg tttgtctgat acatcgacca
     4201 ttttggcgtg gccttgttga ttaatatgag taaactcagt cattttaccc ctcctagtgc
     4261 atctagtata tcatgaaaaa ataaaagttt tggagatgat ttttaatggt agtagaaaaa
     4321 agaaacccaa tcccagttaa agaagcaatt caacgtatcg ttaatcagca gagttcaatg
     4381 ccggcaatta cggtagcact tgaaaaaagt ctaaatcata tcttagcaga agatattgta
     4441 gctacttatg atataccaag gtttgataaa tcaccttatg atggttttgc aattcgcagt
     4501 gttgattcac aaggggcaag tggtcagaat cgcattgagt ttaaagtgat tgatcatatt
     4561 ggtgcaggtt cagtttctga taaattagtt ggggatcacg aagcggtgcg tattatgact
     4621 ggagcacaaa tacctaatgg cgcagatgct gttgttatgt ttgaacaaac gattgaacta
     4681 gaagatacat ttacaattcg taaaccattt tcaaaaaatg aaaatatatc tttaaaaggt
     4741 gaagaaacaa cgacaggcga tgttgttcta aaaaaaggac aagtaattaa tccaggggct
     4801 attgcggtcc ttgcaacata tggctatgca gaggttaaag ttattaagca accgagcgtc
     4861 gctgttattg caacaggaag cgaattatta gatgttaatg atgtattaga agatggaaaa
     4921 attcgtaact ctaatggtcc aatgattcgt gccttagcag aaaaattagg tcttgaagtt
     4981 ggtatttaca aaacacaaca agatgattta gatagtggta tacaagtcgt taaagaagct
     5041 atggaaaaac atgatatagt tattacaacg ggcggagttt ctgtcggaga ctttgactat
     5101 ttacctgaga tttataaggc tgtaaaggcg gaagtgttat ttaataaagt agcaatgcgt
     5161 cctggtagcg taacaacggt tgcatttgca gatggaaaat atttgtttgg attatctgga
     5221 aatccatcag cttgttttac aggatttgaa ctatttgtga aaccagctgt taaacatatg
     5281 tgtggcgcac tagaagtctt cccgcaaata attaaagcaa cattaatgga agattttacc
     5341 aaggcaaacc cattcacacg atttatacgt gctaaagcaa cattaacaag tgctggagct
     5401 actgtagtac cttcaggatt caataaatca ggtgcggttg tagcgattgc acatgctaac
     5461 tgtatggtca tgttaccagg agggtcacgt ggttttaaag cggggcatac agtagatatt
     5521 atattgactg aatctgacgc tgctgaagag gaacttcttt tatgatttta caaattgtag
     5581 gttacaaaaa gtctggtaag acaacattga tgaggcatat tgtctctttc ttaaagtcac
     5641 atggttatac agttgctact attaaacatc atgggcatgg taagaaagat attcaattac
     5701 aggattcaga cgtcgatcac atgaagcatt ttgaagcggg ggcagatcaa agtattgtac
     5761 aaggttttca atatcagcaa actgtaacac gtgtagataa tcaaaatctt actcaaatta
     5821 ttgaaaaatc tgttacaatt gacaccaata tcgtattagt tgaaggcttt aaaaatgctg
     5881 attttgaaaa agtcatagtc tatcgaaatg aagaagagtt gcaagtatta caacaattgt
     5941 cgaatgtttg ttatagcatt aatgttaggg agcatgaaga ttttacagca tttgagcaat
     6001 ggttattaaa taaaattaaa aatgattgtg atacacaatt aacatagagg attgaaatga
     6061 atgaaacaat ttgaaatcgt gacagaaccg atacaaacag aacaatatcg tgaattcact
     6121 ataaatgaat atcaaggtgc agtagttgtt tttaccggtc atgttcgcga atggactaaa
     6181 ggcgtcaaaa cggaatattt agaatatgaa gcgtatattc caatggctga aaagaaattg
     6241 gcacaaattg gagatgaaat aaatgaaaaa tggcctggaa cgataacgag tattgttcat
     6301 agaatagggc cattacaaat ttcagatatc gctgtattaa ttgcaatttc ttcaccgcat
     6361 cgtaaagatg cctatcgagc aaatgaatat gcaattgagc gtataaaaga aattgttccg
     6421 atttggaaaa aagaaatttg ggaagatggt tcaaaatggc aagggcatca aaaagggaat
     6481 tatgaagaag cgaagaggga ggaataagag agatgaaggt actttacttc gcagaaatta
     6541 aagatatatt acaaaaagca caggaagata ttgtgcttga acaagcattg actgtacaac
     6601 aatttgaaga tttattgttt gaacgttatc cgcaaatcaa taataaaaag tttcaagttg
     6661 ctgtaaatga ggaatttgta caaaaatcag atttcattca acctaatgat actgttgcat
     6721 taattccacc ggttagtgga ggttaagcga gcatgaaagc aataattctt gcaggtggtc
     6781 attcagtacg atttggtaag cccaaagctt ttgcggaagt gaacggtgag accttttata
     6841 gtagagtaat taagacatta gaatcaacaa atatgttcaa tgaaattatt attagtacaa
     6901 atgcgcaatt ggcaacgcaa tttcaatatc caaatgttgt tatagatgat gagaatcata
     6961 atgataaagg tccattagca ggaatttata caatcatgaa gcaacatcct gaagaagaat
     7021 tgttttttgt cgtttctgtt gatacaccaa tgattactgg taaagctgta agcacgttgt
     7081 atcagttttt agtttctcat cttattgaaa atcatttaga tgtcgcagct tttaaagaag
     7141 atggacgttt tattccaaca attgcatttt atagtccgaa tgcattaggc gctataacta
     7201 aagcactaca ttctgataat tacagtttta aaaatgtata tcatgaatta tcaacggatt
     7261 atttggatgt cagggatgta gatgcgccct catattggta caaaaatata aattatcagc
     7321 atgatttgga cgctttaatt caaaaattgt aagccgttag gaggtccaca aatggtagaa
     7381 caaataaaag ataaactagg acgtcccatc cgtgacttac ggttatctgt gacagatcgg
     7441 tgtaacttta ggtgtgatta ttgcatgcct aaagaggtat ttggagatga tttcgtattt
     7501 ttacctaaaa atgaactgtt aacgtttgat gaaatggcta gaatcgctaa ggtatatgca
     7561 gaattaggtg taaaaaaaat acgcattaca ggtggagaac cattgatgcg acgcgattta
     7621 gatgtactta tagctaaatt aaatcaaatc gatggtattg aagatattgg tttgactaca
     7681 aatggtttgt tattaaaaaa gcatggacaa aagttatatg atgctgggct acgcagaatt
     7741 aatgtcagtt tggatgctat tgatgatacg ctatttcaat caatcaataa tcgtaatatt
     7801 aaagcgacta cgattttaga acaaattgat tacgcgacgt ctattggttt gaatgtaaaa
     7861 gtaaatgttg ttatacaaaa aggtattaac gatgatcaaa ttataccaat gcttgaatat
     7921 tttaaagata aacatataga gattcgattt atagaattta tggatgttgg taatgataat
     7981 ggatgggatt tcagcaaagt tgtaactaaa gatgaaatgc ttactatgat agagcagcac
     8041 tttgaaatcg atcgtgtaga accaaaatat tttggggaag tagcaaaata ttatcgccat
     8101 aaggataatg gtgttcaatt tggtttgatt acaagtgttt cacaatcatt ttgttctaca
     8161 tgtacacggg caaggctgtc atcagatggg aagttttacg gatgtttatt tgcaactgtc
     8221 gatggattta acgttaaagc gtttattcgt tctggcgtga ccgacgaaga gttaaaagaa
     8281 caatttaaag ctctatggca aataagagat gatcgatatt cagatgagag aactgctcaa
     8341 acagttgaca atcgccaacg taaaaagata aacatgaatt atattggtgg ttaatgtgta
     8401 ggaaccacta tatattatat cattagagat tctttaatat ttctgtctta ctccctaaaa
     8461 tacaatatta tttattaaag taaaaacggt catatctatg ccagatttaa tagaaatgat
     8521 cgtttttaat gtttttacaa attggcgggg ccccaacaca gaagcagacg aaaagtcagc
     8581 ttacaataat gtgcaagttg gggaacgggg ccccaacata gagaatttca aaaagaaatt
     8641 ctacagacaa tgcaggttgg ggaacaacga aataaatttt atgaaaatat catttctatc
     8701 ccactcttaa gaatcactac ataataaatc tttagtggtt ctttaacatt gatgtcacac
     8761 cccatgtcat tgagttgtaa tatatctttt ttaggtataa atgttgtcga ataaacaaca
     8821 agttgtccaa aatatataaa tctaaacaag atatatccag aaatttaata tttgtaatag
     8881 ataaaatgct aagtttgata tataataaat ttaagtaatt gtataataat atgaattaca
     8941 aacatctaag aagaaacata ggaggcatca tattatgagt aataaagttc aacgttttat
     9001 agaagcagaa agggagttaa gtcagttaaa gcactggtta aaaacaacac ataagatttc
     9061 aattgaagaa tttgtagtac tttttaaagt gtatgaagct gaaaagatta gcggtaaaga
     9121 attgagggat acattacatt ttgaaatgct atgggataca agtaaaatcg atttgattat
     9181 ccgtaaaatc tataaaaaag agcttatttc taaattgcgt tctgaaacgg atgaaagaca
     9241 agtattctat ttctatagta cttctcaaaa gaaattgtta gataaaatta ctaaagaaat
     9301 agaagtgtta agcgttacaa actaaaaact taaaaagcat gccaatcagt acatcataat
     9361 tgcgtcttgg ggacagacaa atgatgaata gagattggca tgctttttat ttttgaatat
     9421 aaatatttag ttcatggcat ttctagttac atgacgtcca tgaattaaga agtaaacaag
     9481 catagtaatg attgctaaag cggccataaa gccgaagatt tcactatatg aaaacatatg
     9541 agtaaataac ccaaggaatg atggaccgaa gccgacacct gcatctagac caacgtaaaa
     9601 agtagatgtc gcgataccat atttaatcgg aggtgagact tttatcgcaa tagattgcat
     9661 tgcagatgat aaatttccat accctaaacc taggcaagct ccagcaagta atattaacca
     9721 gctttgatag cttgaaatta agcacacaaa tgaaaggaaa agcatgataa atgctgggta
     9781 gacaataata ttttcatttt tatcatccat caatctacca gcaataggtc tagtaattaa
     9841 cgatgctata gcatagcaaa taaagaaata gcttgctgca gttactaggt gtcgctctaa
     9901 agcaaatgct tgtaaataag ttaggatgga cgcataggta acgccaatta aaagcataat
     9961 tacagcaaca ggaatggcct cttttgcaat aaattgatga atactaaatc ttggtttatc
    10021 aatgacatta gtttcagttt tattatttgt tacttcaaaa tcaactttta taaataatga
    10081 gataatgagt ccgagtatgc ctaatatgac acaaataata aacagtaaat caattgcgta
    10141 ttttgtaata agtaacatgc ctagaaatgg gccaatcgct gtacctaata ctaaacttaa
    10201 agaaaataaa ctgatgcctt cactttttct attaacaggg gtaacgtatg ccgcaatagt
    10261 acctgttgca gttgtcacaa ctgcagttgc gataccgttt atgagacgta caaagattaa
    10321 aaaagctaaa gatccatcaa taaaataaag taattgcgtg ataattaaag caattaaacc
    10381 aataaataat aatcgtttag gtccgatttg atttacaaat ttacctgtag caaatcgacc
    10441 aattaaagac ccgacaataa atagtcctac aactaatccg gctaatgaat cggaagcgtc
    10501 gaaagcttgc ttactatagc cagcaataac tacaagtaac aaatacatgc ataggtatac
    10561 gataaagttg acaaaaaagt tgattgtaaa acttttggtg aaaataggtg atttatttaa
    10621 tgagccttga ttagtcattg tgtgcctcct ttaataatat ttcatgaatt tgattcatca
    10681 catttatggt ttcttgcatt tgatcttgcg aaatgtttgt tttggcaact actgcttgtt
    10741 ggagcttttg tacatcaaga caaacaatct catataattc atgcccttta ggtgttaaag
    10801 ttaaaaactt ttgtcgttta tcctctaaac tgttttctac tgttataagg tcattttcta
    10861 ttagagcttt taaagttttt cttgcagtag gcttttcaat tgcacgtcta tgtgaaattt
    10921 caatgagagt agtgggctga tgtttagcga tatcgcgtaa gattaaccat tggccataat
    10981 atatattgtg tttttctaaa atcggctcgg ctaattttaa ataggggcga tatattgtta
    11041 taaaactatt gaaaaattct tgtgatagca tagtgacatc tcctaagata aaatagttag
    11101 cttagctaac cttttttaca acaatagtaa ttataaaacg ggagcaatta gaaatcaata
    11161 tataattatt aagagcaaaa ataattatac
//
LOCUS       DAHHOJ010000086        11165 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.086, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000086 DAHHOJ010000000
VERSION     DAHHOJ010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11165)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11165
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.086"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            140..856
                     /locus_tag="NBH32_001970"
     CDS             140..856
                     /locus_tag="NBH32_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1515552.1"
                     /translation="MGRKWNNIKEKKAQKDKNTSRIYAKFGKEIYVAAKSGEPNPESN
                     QALRLVLERAKTYSVPNHIIEKAIDKAKGAGDENFDHLRYEGFGPSGSMLIVDALTNN
                     VNRTASDVRAAFGKNGGNMGVSGSVAYMFDHVATFGIEGKSVDDILETLMEQDVDVND
                     VIDDNGLTIVYAEPDQFAVVQDALRAAGVEEFKVAEFEMLPQTDIELSEADQVTFEKL
                     IDALEDLEDVQNVFHNVDLK"
     gene            856..1329
                     /locus_tag="NBH32_001971"
     CDS             856..1329
                     /locus_tag="NBH32_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCD1515553.1"
                     /translation="MKTAEQWINELQLESHPEGGFYRETIREVLKDGHRAPFSSIYFL
                     LTDDNISHFHRIDADEVWYYHAGDSLTIHMINPDGEYMTATLGTDIQNGDVLQYVVPK
                     GTIFASSIENSNTFSLVGCMCQPAFEFKQFELFKQSELFDQYPHLKSVIEKYALK"
     gene            1422..1538
                     /locus_tag="NBH32_001972"
     CDS             1422..1538
                     /locus_tag="NBH32_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515554.1"
                     /translation="MLQMFLMKIALNDFENDKSVLFIFLKNHFYEEMCVLSN"
     gene            1683..2330
                     /locus_tag="NBH32_001973"
     CDS             1683..2330
                     /locus_tag="NBH32_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000865623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HCD1515555.1"
                     /translation="MKVKYIDKRHWRRLIDREYTEVKVNNNRFKGIIGLVTMKKVRDP
                     LEVTVVGQNIIVADDNYKWLQILPEKKRYSITVMFDNKGNPLEYYFDINIKNITQKGN
                     ARTVDLCLDVLALPSGEYELVDEDDLMFALESEQITKKQFHEAYMIAHQIMAELENDF
                     KGFQKKIMYCFNKINAKAQKNHQKPQNKTNFEKSKQIKPKQYNQTKNHQQQKKTK"
     gene            2444..3310
                     /locus_tag="NBH32_001974"
     CDS             2444..3310
                     /locus_tag="NBH32_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1515556.1"
                     /translation="MKIEDYRLLITLDETKTLRKAAEILYISQPAVTQRLKAIENAFG
                     VDIFIRTKKQLITTTEGTMIIEHARDMLKRERLFFDKMQAHIGEVNGTISIGCSSLIG
                     QTLLPEVLSLYNAQFPNVEIQVQVGSTEQIKANHRDYHVMITRGNKVMNLANTHLFND
                     DHYFIFPKNRRDDVTKLPFIEFQADPIYINQIKQWYNDNLEQDYHATITVDQVATCKE
                     MLISGVGVTILPEIMMKNISKEQFEFEKVEIDNEPLIRSTFMSYDPSMLQLPQVDSFV
                     NLMASFVEQPKA"
     gene            3649..4869
                     /locus_tag="NBH32_001975"
     CDS             3649..4869
                     /locus_tag="NBH32_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter"
                     /protein_id="HCD1515557.1"
                     /translation="MDMFAALLQIKNYKLFVANMFLLGMGIAVTVPYLVLFATKDLGM
                     TTNQYGLLLASAAISQFTVNSIIARFSDTHHFNRKIIIILALLMGALGFSIYFFVDTI
                     WLFILLYAIFQGLFAPAMPQLYASARESINVSSSKDRAQFANTVLRSMFSLGFLFGPF
                     IGAQLIGLKGYAGLFGGTISIILFTLVLQVFFYKDLNIKHPISTQQHVEKIAPNMFKD
                     KTLLLPFIAFILLHIGQWMYTMNMPLFVTDYLKENEQHVGYLASLCAGLEVPFMIILG
                     VLSSRLQTRTLLIYGAIFGGLFYFSIGVFKNFYMMLAGQVFLAIFLAVLLGIGISYFQ
                     DILPDFPGYASTLFSNAMVIGQLGGNLLGGAMSHWVGLENVFFVSAASIMLGMILIFF
                     TKNQKITKEDVIST"
     gene            4866..5354
                     /locus_tag="NBH32_001976"
     CDS             4866..5354
                     /locus_tag="NBH32_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HCD1515558.1"
                     /translation="MTIILWLLIIAAFMLAFVGLIKPIIPSVLVLWVGFLIYQFGFHN
                     QHLSWVFYVSMALLTILILCADFLANKYFVNRFGGSKFGEYAALIGVVIGCFVLPPFG
                     IIIIPFILVFIVELIQGYSFERAVKVSIGSIVAFLTSSIAQAIIMFIMIVWFFIDALL
                     IN"
     gene            complement(5453..6142)
                     /locus_tag="NBH32_001977"
     CDS             complement(5453..6142)
                     /locus_tag="NBH32_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 family protein"
                     /protein_id="HCD1515559.1"
                     /translation="MKSTAQLTKENNVKSLRLSNTDREIFENYMTYMRSDFRVNPHDT
                     ELIINRILKQLLSAEQHGLLALDFFNHDPKAHAIKELKAMPNETFKNIFKYIYQHIVL
                     LIGIVSFLKGFLGFFMEKSGSNLYFVSFPFSVVVGFFIVFLFIWFSFKTIQLQCFNNS
                     NWIWIFTYLAIILLIVGFFYVFFIPQSLLAFGPYIQVSNWVFIIFSFIVMPIGLRIER
                     NISKKHSHTFL"
     gene            6269..6712
                     /locus_tag="NBH32_001978"
     CDS             6269..6712
                     /locus_tag="NBH32_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515560.1"
                     /translation="MRTLNKDEHNYIKQIANIHETLLSQVESNYKCTKLSIALRYEMI
                     CSRLEHTNDKIYIYENEGQLIAFIWGHFSNEKSMVNTELLYVEPQFRKLGIATQLKIA
                     LEKWAKTMNAKRISSTIHKSNLPMISLNKDLGYQVSHVKMYKDID"
     gene            6779..7168
                     /locus_tag="NBH32_001979"
     CDS             6779..7168
                     /locus_tag="NBH32_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515561.1"
                     /translation="MKKLIISIMAVMLFLTGCGKSQEKATLEKDIDNLQKENKELKDK
                     KEKLQQEKEKLADKQKDLEKEVKDLKPSKEDNKDDKKDEDKNKDKDKEASQDKQSKDQ
                     TKSSDKDNHKKPTSTDKDQKANDKHQS"
     gene            7306..7605
                     /locus_tag="NBH32_001980"
     CDS             7306..7605
                     /locus_tag="NBH32_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515562.1"
                     /translation="MHEQDFRILEGQDITLPELGRELENITGRTIADSTGEIKRVIAH
                     LPNFESDTDTFVATYRLNHQQDFIDATFTALKSDRARLKEVPVHVELISYISKSK"
     gene            7685..8227
                     /locus_tag="NBH32_001981"
     CDS             7685..8227
                     /locus_tag="NBH32_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515563.1"
                     /translation="MIYCETERLILRDWHEDDLLPFQKMNANYDVRKYFPSLLSYRRS
                     ELDMRTMDAVIKDYGIGLFAVEDKESHQWIGFIGLNYIPETSDYPFKELPLYEIGWRL
                     LPEFWGKGLATEGAKVALKLAEQHQIYDVYSFTAEANKASQRVMEKIGMTVYDHFELP
                     NLSKYHLLKRQVRYYINLRK"
     gene            complement(8323..8889)
                     /locus_tag="NBH32_001982"
     CDS             complement(8323..8889)
                     /locus_tag="NBH32_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HCD1515564.1"
                     /translation="MKRIAVYCGASKGHDPSYVQKAYDLGKYFAEQGYELVFGAGSIG
                     IMGAIQDGVLDHGGKAIGVMPKMLDEHEITSQRLTELILVDSMHERKNKMAELADAFV
                     MAPGGAGSLEEFFEMYSWAQIGIHEKPIAIYNINGFFNPLQTMIDHMIEEGFIDPKYR
                     ALAPLCDTKESLIESILNFKPLGTRSYD"
     gene            complement(8891..9349)
                     /locus_tag="NBH32_001983"
     CDS             complement(8891..9349)
                     /locus_tag="NBH32_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCD1515565.1"
                     /translation="MTHIIIDGDACPVVDSIIDLTTETGIFVTIIRSFSHFSNQLYPP
                     HVSTLYVDDGPDAVDYKIVQLSTKDDIVVTQDYGLASLLVDKVLIVMHHNGKIYNSKN
                     IQQLLDKRYMNAQIRKQGGRHKGPPPFTKQDQKVFEQSLLKVIHRIKELD"
     gene            complement(9352..10035)
                     /locus_tag="NBH32_001984"
     CDS             complement(9352..10035)
                     /locus_tag="NBH32_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515566.1"
                     /translation="MDKKKVIKFMINVLPIVLVPLIVERKRIKQHPDVQKVTDATSKV
                     ASKTSAAISNTASDVKEYVGDKKQDFENKRELKKFAREHDPAYIEKKGEKLAKQNRKD
                     ADKMNKILQKNIEKRHKEEQKAREKNEIQRVKDMKKSQKYEEKAGLTPNKLDEKTEKK
                     GDKLAEKNRKEIAKMNKKLQKNIEKRHKEEQKRQQEADKARIKSFKKYKDYVAKSASQ
                     QNKENNTEA"
     gene            complement(10207..11082)
                     /locus_tag="NBH32_001985"
     CDS             complement(10207..11082)
                     /locus_tag="NBH32_001985"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCD1515567.1"
                     /translation="MFIIELIKGIILGVVEGLTEFAPVSSTGHMILVDDMWLKSSEFL
                     GSQSAFTFKIVIQLGSVFAAAWVFRERFLEILHIGKHKHVEGENDQQRRSKPRRLNLL
                     HVLVGMVPAGILGLLFDDFIEEHLFSVPTVMIGLFVGAIYMIIADKYSAKVKNPQTVD
                     QINYFQAFVIGISQAVAMWPGFSRSGSTISTGVLMKLNHKAASDFTFIMAVPIMLAAS
                     GLSLLKHYQDIQIADIPFYILGFLAAFTVGLIAIKTFLHLINKIKLIPFAIYRIVLVI
                     FIAILYFGFGIGKGI"
BASE COUNT         3926 a         1583 c         1927 g         3729 t
ORIGIN      
        1 tttatacata ttttaaaact aagtaacaga ttgaagaaat cgtagttcaa taatgttaat
       61 tgtgaaaatg tatataatca taaaaaaatc atgtataata tatgttgtta attaaacagt
      121 tcgaaagcga gatgacatta tgggacgtaa atggaataac attaaagaaa aaaaggccca
      181 aaaggataaa aatacaagta gaatatatgc gaaatttggt aaggagattt atgttgcagc
      241 aaaatctggt gaaccaaacc cagaatctaa ccaagcttta agattggtac ttgaacgcgc
      301 taagacatat tcagtaccga atcatattat tgaaaaagca atagataaag ctaagggtgc
      361 tggagacgaa aactttgatc acctaagata tgaaggattt ggcccaagcg gatcaatgct
      421 tattgttgat gcgttaacaa acaatgtaaa tcgtactgca tctgatgtgc gtgctgcttt
      481 tggtaaaaac ggcggtaata tgggtgtatc tggatcagtt gcttatatgt ttgaccatgt
      541 ggcaacgttt ggtattgaag gtaaatctgt tgacgatata cttgaaacat taatggaaca
      601 agatgtagat gtaaatgatg tgattgacga caatggattg acaatagtct atgctgaacc
      661 agatcaattt gcggttgttc aagatgcgct tcgtgcagct ggtgtagaag aatttaaagt
      721 agcagaattt gaaatgttac ctcaaacaga tattgagctt tctgaagcgg accaagtaac
      781 atttgaaaaa ttaatcgatg cattagaaga tttagaagat gtacaaaacg tattccataa
      841 tgtggatttg aaataatgaa aacagcagaa caatggatta atgaattaca actcgaatcg
      901 catcctgaag gtggttttta tagagagaca atccgagaag tattaaaaga tggacacaga
      961 gcgccgttta gcagtattta ttttttactt acagatgaca atatttcgca ttttcatcga
     1021 attgatgctg atgaagtatg gtactatcat gccggtgatt ctctaacaat tcatatgata
     1081 aatccggatg gagaatatat gactgcaaca ttgggtactg atatccaaaa tggagatgta
     1141 ttgcaatatg tagtgcctaa aggaacaatt tttgcttctt caattgaaaa ttcaaatact
     1201 tttagtttag taggttgtat gtgtcaaccg gcatttgagt ttaagcaatt tgaattgttt
     1261 aagcaatctg aattatttga tcaatatccg catcttaaat cagtaatcga aaaatatgct
     1321 ttaaaataaa aaagaatcaa tgaagtggtt tgaaggttgt taataaacct ttgagtcact
     1381 tcatttttat atgtattctt gattgaatca gaatagattt gatgcttcag atgtttttaa
     1441 tgaaaatagc attaaatgat tttgaaaacg ataagagtgt gttatttata tttttgaaaa
     1501 atcactttta tgaagaaatg tgtgtactat ctaattaata ttgtctattt taagtaaatt
     1561 gaagcgagtt gtagtaaaat aataataaat attttcatat gattaacaaa aactataaaa
     1621 ctgtatcatg acatgacatc atttatgtag ttttatataa taatgacaag gagttggtga
     1681 ctgtgaaagt aaagtatata gataaacgtc actggcgtcg cctaattgat agggaataca
     1741 cagaggtaaa agttaataat aataggttta agggtattat aggcttagtc acgatgaaaa
     1801 aggttcgtga tcctttagag gtgacggtag ttggacaaaa tatcattgtc gcagatgaca
     1861 attataaatg gttgcaaata ctacctgaaa agaaacgtta tagtataact gtaatgtttg
     1921 ataataaagg caatccatta gaatattatt ttgatataaa tatcaaaaat ataacgcaaa
     1981 aaggcaatgc gcgtacagta gatttatgtt tagatgtttt agcattacca agtggtgaat
     2041 atgagttggt agatgaagat gatttaatgt ttgcattaga aagtgagcaa attacaaaaa
     2101 agcaatttca tgaagcatat atgattgcac atcaaattat ggcagagtta gaaaatgatt
     2161 ttaaaggatt ccaaaagaaa atcatgtact gctttaataa aattaatgca aaggctcaaa
     2221 aaaatcatca aaagccacaa aataaaacta attttgaaaa aagcaaacaa ataaaaccta
     2281 agcaatataa tcaaactaaa aatcaccaac aacaaaagaa aactaagtaa ttcaagctgc
     2341 agccatacca ataaaattgg tgaaaattat tcgttagtaa tgaactcttg aatgctcgta
     2401 gtcatgaaag ttcaataatt gaataattta tgggggggaa attatgaaga ttgaagacta
     2461 tcgtttacta ataacattag acgaaacgaa aacgttacgt aaagcggctg aaattttata
     2521 tatatctcaa cctgctgtta cacaaagact aaaagctatt gaaaatgctt ttggagtaga
     2581 tatttttatc agaacaaaaa aacaattgat tacaacaact gaaggaacaa tgattattga
     2641 gcatgcccgt gacatgttga aaagagagcg attatttttt gacaaaatgc aggcacatat
     2701 tggtgaagtg aatggaacaa tatcaatcgg gtgttcttct ttgattggac aaaccttact
     2761 tcctgaagtt ttgagcctat ataatgccca atttcctaat gttgaaatac aagtgcaagt
     2821 tggttcaact gaacaaatta aagcaaatca tagagattat catgttatga taactcgtgg
     2881 aaataaggta atgaatttag ctaacacaca cttatttaat gatgatcatt attttatttt
     2941 tccaaaaaat agacgagatg atgttacaaa gttaccattt atagagtttc aagctgaccc
     3001 gatttatata aatcaaataa aacaatggta taacgataat ttagaacaag attaccatgc
     3061 aactattaca gtggatcaag tagcaacttg caaagaaatg ttgattagtg gtgtaggtgt
     3121 tacaatcttg ccagaaatta tgatgaaaaa tatcagcaaa gaacaatttg agtttgaaaa
     3181 agtagaaatt gataatgaac cgctgattcg ttcgacattt atgagttatg atccgagcat
     3241 gttgcaattg ccacaagttg attcgtttgt aaatctcatg gcgagctttg ttgaacaacc
     3301 aaaggcgtag tcttaaacta atttaaggtt agtatttaat tttaaactat tcggttaagt
     3361 tgaacgcagt tgtttgctaa tgcaccaaca gcaaaagagc ccctaattaa taaattaaaa
     3421 ggggacaaag gaatacagtt ggttgctaat gcaccaactg cataagagcc cctaattaat
     3481 aaattaaaag gggacgaagg attacagttg attgctaacg caccaacagc aaaagagccc
     3541 ctaattaata aattaaaagg ggataaagga ttacagttga ttgctaacgc accaactgca
     3601 taagagcccc taattaataa attaaaaggg gctctaagaa gtggggttat ggatatgttt
     3661 gcagcgttat tacaaataaa gaattataaa ctctttgttg ctaatatgtt tctactaggt
     3721 atgggtattg cggttacggt cccatatctt gttctttttg caactaaaga tttaggtatg
     3781 acaacaaatc agtatggatt acttctagca tctgcagcga ttagccagtt tacagtaaat
     3841 tcaattattg ctagattttc ggatacgcat cactttaata gaaaaattat tattattctc
     3901 gcattattaa tgggtgcgct tggtttttca atatactttt ttgtagatac aatctggtta
     3961 ttcatattac tatatgcgat tttccaagga ttatttgcac cagcaatgcc ccaactttac
     4021 gcatctgcta gagaatctat caatgtttca agctctaaag atagagctca atttgccaac
     4081 acagtattac gttcaatgtt ctcattgggc tttttatttg gtccatttat tggtgcccaa
     4141 ttaatcggat taaaaggcta tgctggattg tttggtggaa caataagtat cattttattt
     4201 actttagtac ttcaagtgtt tttctataag gatttaaaca ttaaacaccc tattagtacg
     4261 caacaacatg ttgaaaaaat tgctcctaat atgtttaaag acaaaacact tttattacca
     4321 tttattgcat ttattttatt acacattgga caatggatgt atacgatgaa tatgccttta
     4381 tttgttactg attatttaaa agaaaatgag caacatgtcg gttatttagc tagtttatgt
     4441 gctggtttag aagtgccatt tatgatcatt cttggcgttt tatcatctag attacagact
     4501 cgaacattgt tgatttatgg agcgatattt ggtggtttat tctacttcag cattggggta
     4561 tttaaaaact tctatatgat gttagcagga caggtgtttt tagctatttt cttagcggtt
     4621 cttttaggaa ttggtattag ttatttccaa gatatcttac cagatttccc aggatacgcc
     4681 tcaacactat tttctaatgc aatggttatt ggacagttag gcggtaactt attaggtggt
     4741 gctatgagtc actgggtagg tttggaaaat gtattttttg tatcagcagc atcaatcatg
     4801 ttaggtatga tacttatatt ctttactaaa aatcaaaaaa ttacaaaaga ggatgtgata
     4861 tcaacatgac aattatttta tggctactta tcattgctgc cttcatgtta gcatttgttg
     4921 ggttgattaa gccgattatt ccttctgttt tagtattatg ggttggcttt ctaatctatc
     4981 aatttggctt tcataatcag catttatcat gggtgtttta tgtatctatg gcattgctaa
     5041 caatattaat tttatgtgcg gactttttag ctaataaata ttttgtgaat cgcttcggtg
     5101 gttctaagtt tggagagtat gcagctttaa ttggtgtggt tattggatgt tttgttttac
     5161 cgccatttgg aattattatt atacctttta ttttggtatt catagttgaa ttaatacaag
     5221 gctattcatt tgaaagagca gttaaagtaa gtataggttc aatcgtagca tttttaacaa
     5281 gtagtatagc tcaagcaatc attatgttta taatgattgt atggttcttt atagatgctt
     5341 tattgattaa ttaataaaaa gcttattgca aaatatgttt ttcggtaact gtaattttgt
     5401 gattttatca tgaacagtac caaattcgca tatgatgtaa taagctttta gtttataaaa
     5461 atgtatgaga atgttttttc gaaatatttc tttcaatgcg taatccaata ggcataacaa
     5521 taaatgaaaa tataataaat acccaattgc ttacctgaat atatggtcca aaggcaagta
     5581 atgattgtgg aataaagaat acatagaaaa atcctacaat tagtagaatg attgctaaat
     5641 aggtgaatat ccatatccaa tttgaattgt taaaacactg caattgtatt gttttaaaac
     5701 taaaccatat aaataaaaag acaataaaga atccgacgac tactgaaaac gggaacgaaa
     5761 caaaatataa attacttcca cttttttcca tgaaaaatcc taaaaatcct ttgagaaaac
     5821 taacaatccc aattaataga acgatgtgct gatatatata tttaaaaata tttttaaatg
     5881 tttcattagg catcgctttt agttctttta ttgcatgtgc ttttggatcg tgattgaaaa
     5941 aatctaaggc taataaacca tgttgttctg cgcttaataa ttgtttaagt atacggttaa
     6001 taattaactc tgtatcatga ggattgacgc gaaagtcaga gcgcatataa gtcatataat
     6061 tctcgaagat ttctctatca gtattgctta atcttaatga tttaacatta ttttcttttg
     6121 ttaattgcgc agtacttttc attgttaatt aagcgctcct ttaaaaatgt ttaattccaa
     6181 attaaaatgg aaatgatttt atagtattaa taaggtcaat catatcatat taaacgcata
     6241 aatataacga ttaatattgg agaggaaaat gaggacactt aataaagatg aacataatta
     6301 tatcaagcaa atagctaata tacatgagac attattgtcg caagtagaat ccaactataa
     6361 atgtactaaa ctgagtattg ctcttaggta cgagatgata tgttcaagat tagaacatac
     6421 aaatgataaa atttatatat atgaaaatga aggtcaatta atagcgttta tttggggaca
     6481 ttttagtaat gaaaaaagta tggttaacac tgaactgcta tatgttgaac cacaatttcg
     6541 caaactggga atagctacgc aactgaagat tgcgcttgaa aaatgggcaa aaactatgaa
     6601 tgcaaagcga ataagcagta caattcataa aagtaattta ccaatgatat ctttgaataa
     6661 agatttaggt tatcaagtga gtcatgtgaa aatgtataaa gatattgatt agaattagga
     6721 ttatgttgct aattcatgtt aaaattaaaa aagatttaat gacgttaagg agttttatat
     6781 gaagaaatta atcatcagta ttatggcggt catgctattt ttaacaggtt gtggtaaaag
     6841 ccaagagaaa gccactctgg aaaaggacat cgataattta caaaaagaaa ataaagaatt
     6901 aaaagataaa aaagaaaagc ttcaacaaga aaaagaaaaa ttagcagata agcaaaaaga
     6961 ccttgaaaaa gaagtgaaag atttaaaacc ttcaaaagaa gataacaagg atgataaaaa
     7021 agacgaagac aaaaataaag ataaagataa agaggcatca caagataagc aatcaaaaga
     7081 tcaaactaag tcatcggata aagataatca caaaaagcct acatcaacag ataaagatca
     7141 aaaagctaat gacaaacacc aatcataatt gaattgcttt cttgttatag atgaaaggta
     7201 cagcgtttta aaccttattt taagggtatg tattaattaa aatgtggtca tgattgaaac
     7261 agaatgtaaa aatagacaac gtaattaata aaggagagaa acggcatgca tgaacaagat
     7321 tttagaattt tagagggtca agatattact ttgccagaat taggtagaga attagaaaat
     7381 attacaggac gcacgattgc tgattctact ggcgaaatta agcgtgtaat tgcacattta
     7441 ccaaactttg agtccgatac agatactttt gttgctacat atcgtttaaa ccatcaacaa
     7501 gattttatag atgctacttt tactgcgctg aaatcagata gagcacgttt aaaagaagtg
     7561 ccagttcatg ttgaacttat aagttatatt tctaaatcaa aataaactgc tatctaaaac
     7621 gcaaagttga tcaaaatatc gattttgtgt tttttattga gaaattatat aggagtgtca
     7681 atcgatgatt tattgtgaaa cagagcgttt aatattaaga gactggcatg aagatgatct
     7741 gttacctttt caaaaaatga atgcgaatta tgacgtacgt aaatattttc caagtttatt
     7801 gagttatcgt cgttcagaat tagatatgag aactatggat gcggttatta aagattatgg
     7861 cattggatta tttgctgtag aagataaaga gtcccatcaa tggataggct ttataggttt
     7921 gaattatatt ccagaaacaa gcgattatcc atttaaagaa ttaccgcttt atgaaatagg
     7981 ttggcgcttg ttgccagaat tttggggaaa aggattagca actgaaggcg caaaggtagc
     8041 gttgaagtta gcagaacaac atcaaatata cgatgtctat agttttacag cagaagcaaa
     8101 taaagcttca caacgtgtaa tggaaaaaat tggcatgaca gtgtatgatc atttcgaatt
     8161 acccaatcta agtaagtatc atttattaaa aaggcaagtg cgctattaca ttaatcttcg
     8221 aaagtgaaaa atttatacat aagcgtaaca aacaccccta atattgttta gctgatgata
     8281 acaatattag gggtgtttgt ttatttttta accttagaat gattaatcgt atgaacgagt
     8341 acctagaggt ttgaaattca atatagattc tattaatgat tccttagtgt cacataacgg
     8401 tgcaagtgca cgatacttag gatcaataaa accttcctca atcatatggt caatcattgt
     8461 ttgtagtgga ttgaaaaagc cattaatatt ataaatggca ataggctttt catggatacc
     8521 aatttgagcc caactataca tttcgaaaaa ttcttctagt gaacctgcgc cgccgggagc
     8581 catgacaaat gcatctgcaa gttctgccat tttattttta cgttcatgca tagaatcaac
     8641 tagaattaat tcagttaaac gttggcttgt gatttcatgt tcatctaaca ttttaggcat
     8701 gacgccaata gctttgccgc catgatctaa tacaccatct tgaatggcac ccataatacc
     8761 aattgaccct gcaccaaata ctaattcata accttgttca gcaaaatatt tacctaaatc
     8821 gtatgctttt tgtacatatg aagggtcatg acctttgctt gcaccacaat aaactgcgat
     8881 tcgtttcatg ttaatccagc tccttaattc gatgaatgac ttttaatagt gattgttcaa
     8941 acactttttg atcttgcttt gtaaaaggtg ggggaccttt gtggcgacca ccttgttttc
     9001 taatttgtgc attcatatat cgtttatcta atagttgttg aatatttttg gaattgtata
     9061 tcttcccatt atgatgcatg acaattaaga ctttgtcgac taataaactt gcgagtccat
     9121 aatcttgagt gactacgata tcatctttcg ttgataattg aacaattttg taatcaactg
     9181 catctggtcc atcatcaaca tataatgttg atacatgcgg gggatataat tggttcgaaa
     9241 aatggctgaa gctccgaata attgtcacaa aaatgcctgt ctcagttgtt aaatctataa
     9301 tagaatcaac aacaggacaa gcatctccat caataataat atgtgtcaca attatgcctc
     9361 agtattgttt tctttatttt gttgagaggc gcttttggca acataatctt tatatttttt
     9421 aaatgacttg atgcgtgctt tatcagcttc ttgttggcgt ttttgttctt ctttgtgtcg
     9481 tttttcaata tttttttgta actttttatt cattttagcg atttctttgc gatttttttc
     9541 agctagttta tcgccttttt tctcagtttt ctcatctaat ttattaggtg ttaagcctgc
     9601 tttttcttcg tatttttgtg attttttcat atctttaaca cgttgtattt cattcttttc
     9661 gcgggctttt tgctcttctt tatgacgctt ttcgatattt ttttgaagta ttttattcat
     9721 tttatcagcg tctttacgat tttgtttagc taatttttcg ccttttttct caatataggc
     9781 aggatcatgt tctctagcaa actttttaag ttcacgctta ttttcaaaat cttgtttttt
     9841 atcgccgaca tattctttaa catcactcgc tgtgttactg attgctgcag atgtttttga
     9901 agcaacttta cttgtagcat ctgtaacttt ttgtacgtcc ggatgttgtt tgatacgttt
     9961 acgttcaaca attaacggta ccaatacaat tggtaataca ttaatcataa atttgatgac
    10021 ttttttctta tccatagatc ttgcctccat aattacttta ttaattttac ataccctatg
    10081 atacatcaat ataaacgatg atagtagtga atcactatta agtatttcag atgtttttta
    10141 aaagaagaca ataaaaactg ccaatcaagt ggttccttaa ttgacagtct atattttaaa
    10201 aggaaattaa atacctttac caatgccaaa tccgaagtaa agtatagcaa taaagattac
    10261 taatacaatt ctgtaaatgg caaatggaat tagtttgatt ttgttaatta gatgcaagaa
    10321 tgttttgatt gcaattagtc caacagtaaa tgcagctaaa aagcctaaaa tataaaaagg
    10381 tatatcagca atctgaatat cttgataatg ttttaataaa gataaaccac tagctgctaa
    10441 cataattgga acagccataa taaatgtgaa gtctgatgca gctttatgat ttaatttcat
    10501 taatacccca gttgaaattg ttgagcctga acggctgaaa ccaggccaca tagctactgc
    10561 ttgagagata ccaattacaa acgcttggaa ataattgatt tgatctactg tttgtgggtt
    10621 tttaacttta gctgagtatt tatcagcaat aatcatatag atagcaccta cgaataagcc
    10681 aatcataaca gttggcacac taaataaatg ttcttcgatg aaatcatcaa atagtaagcc
    10741 taaaatacct gctggcacca tacccactaa tacatgtaat aaatttaaac gtcttggctt
    10801 tgaacgtctt tgttgatcgt tttctccttc aacatgtttg tgtttaccaa tatgtaaaat
    10861 ctctaagaag cgttcgcgga atacccatgc tgctgcaaag acggatccta attggatgac
    10921 gattttaaat gtaaatgctg attgagaacc taaaaattca gatgatttta accacatatc
    10981 atcaactaga atcatatgtc cagtagagga aacaggtgca aattctgtta atccttcgac
    11041 gacccctaag ataatacctt ttattaattc aatgataaac ataatgtacc cactttcatt
    11101 actcaattta atttatttaa atatcaaaat taccatatca tgatagcata ttcatttaaa
    11161 gacat
//
LOCUS       DAHHOJ010000087        11144 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.087, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000087 DAHHOJ010000000
VERSION     DAHHOJ010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.087"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(56..550)
                     /locus_tag="NBH32_001986"
     CDS             complement(56..550)
                     /locus_tag="NBH32_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515568.1"
                     /translation="MIKIKTLTNNDFNEYKRLVSTVNEEFTQDSHYSQTMTDTLIHDI
                     LNQGSPKCIVFGCYENETLIATAALEQIRYVGKEHKSLIKYNFVTNNDKSINSELINF
                     IINYARQNNYESLLTSIVSNNIGAKVFYSALGFDILGFEKNAIKIGNTYFDEHWLFYD
                     LINK"
     gene            complement(949..1371)
                     /locus_tag="NBH32_001987"
     CDS             complement(949..1371)
                     /locus_tag="NBH32_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HCD1515569.1"
                     /translation="MAIHYETKATNVGGRKGHVYTDDRALDIDIVPPAQADGKATNPE
                     QLFAAVYASCFNGAFDLILKQNKVRDAHPEVTLTVRLEDDPDSESPKLSVSIDATIKN
                     VISQEEAEKYLQMAHEFCPYSKATQGNINVDLNVNVVD"
     gene            1519..2235
                     /locus_tag="NBH32_001988"
     CDS             1519..2235
                     /locus_tag="NBH32_001988"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCD1515570.1"
                     /translation="MTHVEVVATIAPQLSIEETLIQKINHRIDAIDVLELRIDQIENV
                     TVDQVAEMITKLKVMQDSFKLLVTYRTKLQGGYGQFTNDLYLNLISDLANINGIDMID
                     IEWREDIDIEKHQRIITHLQQYNKEVVISHHNFESTPPLDELQFIFFKMQKFNPEYVK
                     LAVMPHNKNDVLNLLQAMSTFSDTMDCKVVGISMSKLGLISRTAQGVFGGALTYGCIG
                     EAQAPGQIDVTDLKAQVTLY"
     gene            2318..2857
                     /locus_tag="NBH32_001989"
     CDS             2318..2857
                     /locus_tag="NBH32_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HCD1515571.1"
                     /translation="MELQQAIANRRSVKKFKRDMHIDDALLYQAIEKAADAPNHGMRE
                     PWRVVHVPKDRLGDMSKDISKFAFPNELDKQQCHYDAVTKLGGMLLLILKTDPRQRQN
                     DENYFAFGAYAQNLMLLLYEAGIGTCWKSPLYIYDPKVRKTLGIKKDEVLAGFLYLTD
                     LEEDMPKAPRKNRNLITLY"
     gene            complement(3007..3327)
                     /locus_tag="NBH32_001990"
     CDS             complement(3007..3327)
                     /locus_tag="NBH32_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1515572.1"
                     /translation="MQAIKSNESFKSVINSDTPVIVKFEAGWCPDCRAMDLWIDPIVE
                     QYNDYQWYTVNRDELEDVVVENEVMGIPSLLVFKNGDKIAHLHSANAKSPEQVESFLA
                     ETFK"
     gene            3471..3827
                     /locus_tag="NBH32_001991"
     CDS             3471..3827
                     /locus_tag="NBH32_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HCD1515573.1"
                     /translation="MIKFYQYKNCTTCKKAAKFLDEYGVSYEPIDIVQHTPTINEFKT
                     IIANTGVEINKLFNTHGAKYRELDLKNKLQTLSEDEKLELLSSDGMLVKRPLAVMGDK
                     ITLGFKEDQYKETWLA"
     gene            3985..4365
                     /gene="gcvH"
                     /locus_tag="NBH32_001992"
     CDS             3985..4365
                     /gene="gcvH"
                     /locus_tag="NBH32_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001831963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCD1515574.1"
                     /translation="MAVPNELKYSKEHEWVKVEGNVATIGITEYAQSELGDIVFVELP
                     ETDDEINEGDTFGSVESVKTVSELYAPISGKVVEVNEELEDSPEFVNESPYEKAWMVK
                     VEISDESQLEALLTAEKYSEMIGE"
     gene            4544..5422
                     /locus_tag="NBH32_001993"
     CDS             4544..5422
                     /locus_tag="NBH32_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1963 domain-containing protein"
                     /protein_id="HCD1515575.1"
                     /translation="MLNIQDVSHLSKKEQKAYNRFVESVENGNLPVLPCIEMDLKEMK
                     EETLNQSKIGGMPFLKSFKDIPLDENNVPMVLLAQINLDDLPEQQELFPVKEGILQFW
                     ISSEDQMYGMSENLKGNNINSRLVYIKEPITDLSLENIQAHLKSLDADNEDIPFSGAF
                     SIEFRLSKQTITCTDYKYDEDVLALWNKVNPSFALKSMFGGYDELMEPVCNTFTAKEP
                     FNQLGGYPYFDQIDPRTNDQELKMYDRVLLQIDSTRDGNSSIIWGDLGIANILVKSTD
                     LEAMKFDDYMYSWDCS"
     gene            5897..6283
                     /locus_tag="NBH32_001994"
     CDS             5897..6283
                     /locus_tag="NBH32_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCD1515576.1"
                     /translation="MAIVNKVIIVEGKSDKKRVQQVIAEPVNIICTHGTMSIDKLDDM
                     IESLYDKQVFVLADSDDEGDRIRNWFKRYLSESEHIFIDKTYCQVANCPKQYLAHVLS
                     KHGFTCKKETPLLPNINNERLVLVNE"
     gene            6276..6572
                     /locus_tag="NBH32_001995"
     CDS             6276..6572
                     /locus_tag="NBH32_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1515577.1"
                     /translation="MNNSLDIKDVTTFYEEDKHLIFGYTPTCGTCKVSERMLDIANEI
                     LQLPLLKIDLNFYPQFCKDMQIMSTPILLLMNKDKEVKRIYAFKSVTDLLENLK"
     regulatory      6630..6733
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            6823..7848
                     /locus_tag="NBH32_001996"
     CDS             6823..7848
                     /locus_tag="NBH32_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein"
                     /protein_id="HCD1515578.1"
                     /translation="MIELKEVVKEYRTKNKEVLAVDHVNLSIRAGSIYGVIGFSGAGK
                     STLIRMFNHLEAPTSGEVIIDGDHIGQLPKNGLRAKRQKVSMIFQHFNLLWSRTVLKN
                     IMFPLEIAGVPRRRAKQKALELVELVGLKGREKAYPSELSGGQKQRVGIARALANDPT
                     VLLCDEATSALDPQTTDEILDLLLKIREQQNLTIVLITHEMHVIRRICDEVAVMESGK
                     VIEQGPVTQVFENPQHTVTKRFVKDDLNDDFETSLTELEPLEKDAYIVRLVFAGSTTT
                     EPIVSSLSTAYDIKINILEANIKNTKNGTVGFLVLHIPYISSVDFGKFEKELIERQVK
                     MEVLRHG"
     gene            7841..8536
                     /locus_tag="NBH32_001997"
     CDS             7841..8536
                     /locus_tag="NBH32_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515579.1"
                     /translation="MGKSFSEIINEMITMPNIQWPEVWTAIVETLYMTVVSTIFAFIL
                     GLILGVLLFLSAKGKSIGARLFYSIVSFIVNLFRAIPFIILILLLIPFTSLILGTISG
                     PTGALPALIIGAAPFYARLVEIAFKEIDKGVIEAAWSMGANTWTVIRKVLLPEAMPAL
                     VSGITVTAIALVGSTAVAGVIGAGGLGNLAYLTGFTRNQNDVILVSTVFILIIVFIIQ
                     FIGDWLTNKLDKR"
     gene            8554..9375
                     /locus_tag="NBH32_001998"
     CDS             8554..9375
                     /locus_tag="NBH32_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1515580.1"
                     /translation="MKKLFGLILVLTFAVVLAACGNGNKSGSDDKKITVGASPAPHAE
                     ILEKAKPLLEKKGYELDIKTINDYTTPNKLLDKGEIDANYFQHTPYLNTEKKDKGYKI
                     VSAGDVHLEPMAVYSKKYKSLKELPKGATVYVSNNPAEQGRFLKFFVDAGLIKIKKGV
                     KIEDAKFSDITENKKDIKFNNKQSAEFLPKIYQNEDADAVIINSNFAIEQKLNPKKDS
                     IAVESAKDNPYANLIAVKEGNQDDKKIKALIEVLQSKDIQDFINEKYNGAVIPAK"
     gene            9726..9920
                     /locus_tag="NBH32_001999"
     CDS             9726..9920
                     /locus_tag="NBH32_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCD1515581.1"
                     /translation="MADESKFEQAKGNVKETVGNVTDNKNLENEGKEDKASGKAKEFV
                     ENAKEKATDFIDKVKGNKGE"
     gene            complement(10154..11005)
                     /locus_tag="NBH32_002000"
     CDS             complement(10154..11005)
                     /locus_tag="NBH32_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HCD1515582.1"
                     /translation="MQQFKWINILKGFAMGTSDLVPGVSGGTIALLLGIYNQFIASIS
                     GIFSRRFWPSFTFLIPIIIGMLLAMGSLSNLFNYLLSQHHIPTMFFFGGLIIGIVPYL
                     LKISNYKTSFTTKHYMMVIAGIAILIVITLMNNGDKHAGETLTLSTGLIIKYFIAGMC
                     ASSAMLLPGISGSFMLLVFGVYGTVMLAISEVVKLNFTGLPILLAVGFGVLAGFIISS
                     KIIQYFLTHHKLMTFALIIGFVVGSLFAVFPGLPTNIVMWFVSLVVFIIGFIVSLTLG
                     RITAENE"
BASE COUNT         4046 a         1528 c         1964 g         3606 t
ORIGIN      
        1 aaatagaacc tatatgattt gaatttatga gaaatgtaga atatataaaa ctgtattact
       61 tattaatcaa atcataaaaa agccaatgtt catcgaaata ggtgtttccg attttaattg
      121 catttttctc aaaaccaaga atgtcgaatc ctagtgcact atagaaaact ttagcaccta
      181 tgttgtttga cacaattgat gtaagtaaag attcgtaatt gttctgccgt gcataattaa
      241 taatgaaatt aatgagctcg ctattaatcg atttatcgtt attagtaaca aagttgtatt
      301 taattaatga tttatgttct tttccaacgt atcgaatttg ttctaaggca gctgttgcga
      361 taagtgtttc gttttcataa cagccaaata caatacattt cggtgaacct tgatttaaaa
      421 tgtcatgtat taaggtgtca gtcattgttt gactataatg tgaatcttga gtgaattctt
      481 cattgactgt cgaaacaagt ctcttatact cattaaaatc attattcgtt aatgttttaa
      541 ttttaatcat tactgacctc cttaatcatt attaagaaag attttgtact aaaaaaattt
      601 cttaattaca tattaaaatg tttattaaaa ttttgcaatc attgatttat atgcacattc
      661 ttgtaaatgg attatcaatt taattatatt tatttttaag ttcaggaaat tatggaattg
      721 gttattggtc gacactggac ttttatttgt agacggtatg cgaagaagtc aagccagtat
      781 tggaaccggt cacatttatg atgactaagg gacaatcatt ttcagttaaa tattgccaga
      841 aatttcaatc acgaatagaa aaaataacca accaaaaatt tttggttggc aaatttgaat
      901 tttatgtgaa ttttattaat aacgttgaat aatctcttta agttaatgct aatctacaac
      961 atttacattt aaatcgacat taatatttcc ttgagtcgct tttgaatatg gacaaaattc
     1021 atgagccatt tgtaaatatt tttcagcttc ttcttgagat ataacatttt taattgtcgc
     1081 atcaattgaa acacttaatt taggactttc tgagtctgga tcatcttcta gtctcactgt
     1141 tagtgttact tctggatgag catcacgcac tttgttttgc tttaaaatta ggtcgaaggc
     1201 accgttgaag caagatgcat aaactgctgc aaataattgt tcggggttag tagctttacc
     1261 atctgcttga gcaggcggaa cgatatcaat atctaatgct cgatcatcag tataaacatg
     1321 tcctttacgt ccgccaacat tcgtcgcttt agtttcataa tgtattgcca tacttataac
     1381 ctcctaattt aaaatacatt tatgctttac ccattcgaaa ataccttaat cattttcatt
     1441 tatagcattg tttgattgaa ggataaaaag ttgttgttta caataaaaat aatgagtatc
     1501 tgaaatgagg gatttactat gacacatgtg gaagtagtag cgactatcgc gccacaatta
     1561 tctatcgaag aaactttaat tcaaaaaatt aatcatcgta ttgatgcaat agacgtatta
     1621 gaattacgaa ttgatcaaat tgaaaatgtc acagttgatc aagtggcaga aatgattaca
     1681 aagctgaagg ttatgcaaga ttcattcaaa ttattggtta cgtatcgtac aaagttacaa
     1741 ggtggctatg ggcaatttac aaatgacttg tatcttaatt taatatcaga cttagcaaat
     1801 atcaatggca tagatatgat tgatatagaa tggcgagaag atattgacat tgaaaaacat
     1861 caaagaatca ttacacattt gcaacagtat aataaagagg tggtcatatc acatcataat
     1921 ttcgaaagta cgcctccatt agatgaattg caatttatat tttttaaaat gcaaaaattc
     1981 aacccagaat acgttaaatt agcagtaatg ccacataata aaaatgatgt gttaaattta
     2041 ttgcaggcaa tgtctacatt ttcagatact atggactgca aagttgttgg tatttcaatg
     2101 tctaaacttg gactaataag tagaacggct caaggcgttt ttggtggtgc gttgacttat
     2161 ggttgtatcg gagaagcaca agctccagga cagattgatg ttactgattt aaaagcacaa
     2221 gtgactttat actaataaaa cgaattaggt tgcttttgtg taagtgctgt cttataattg
     2281 agaagtatta tcattgttcg tggaggagcg attttagatg gaattacaac aagcaatagc
     2341 taatagaaga agtgtgaaaa aatttaaaag agatatgcac atagatgacg cattgctata
     2401 tcaagcaatt gagaaagctg ctgatgctcc aaatcacgga atgagggaac catggagagt
     2461 tgtgcatgtt ccgaaagaca gattaggaga tatgagtaag gatatttcta aatttgcatt
     2521 tcctaatgaa ttagataagc aacaatgtca ttatgatgca gtaacgaaac ttggtggcat
     2581 gttattgctt attttaaaaa cagatccaag acaacgtcaa aatgatgaaa actactttgc
     2641 atttggtgca tatgcacaaa atcttatgtt gttactttat gaagcgggaa taggtacatg
     2701 ttggaaatcg ccattatata tctatgatcc taaagtaaga aaaacacttg gtataaagaa
     2761 agatgaagtt cttgctggat tcttatattt aacggattta gaagaagata tgcctaaagc
     2821 accacgtaaa aatagaaact taattacatt atattaatat gtataattat agaaacatta
     2881 ataaaagctg agtcatgaat tgatggacat ctatcgagtt agagatttaa tctaacttac
     2941 tagagtcggt acaatcacag tctcagcttt ttattgtgca gtatatacac atttttattt
     3001 tagtatttat ttaaaagttt ctgctaaaaa tgattcaact tgttcaggtg acttagcatt
     3061 tgctgaatga aggtgtgcaa ttttgtcgcc gtttttaaat actagcaagc tagggatacc
     3121 cataacttca ttttcaacaa ctacatcttc taattcatca cgattaacag tataccattg
     3181 gtaatcatta tattgttcta cgattgggtc aatccataaa tccatagcac gacagtctgg
     3241 gcaccatcct gcctcaaatt taacaattac aggtgtatcg ctattaatta cagatttaaa
     3301 tgattcatta cttttgattg cttgcattgt aacaactcct ctagatagtt taataatttt
     3361 tattatagct aaatttataa cataataaaa aattttagct tcaaaatgaa agccttttgc
     3421 tatggaaaat gatatattta ttttaaatac ataaaggagg ttgcagtcgt atgattaaat
     3481 tttaccaata taagaattgt acaacttgta aaaaggcagc aaagttttta gatgaatatg
     3541 gcgtaagtta tgaaccaatt gatatcgttc aacatacacc tacaataaat gaatttaaaa
     3601 caataattgc aaatacaggc gtagaaatta ataaattgtt taatacacac ggcgcgaaat
     3661 atcgtgagct tgatttgaaa aataaattac aaaccttatc agaagatgaa aagttagagt
     3721 tgttatcatc tgatggtatg ttagtaaagc gtcctctagc agtaatgggc gataagataa
     3781 cattaggatt taaagaagat caatataaag agacttggtt agcgtaagtg aaatgtaaac
     3841 gtttactaaa tatctcgata tttagattca ttacatgtaa aatgaaataa gctatacaat
     3901 tgttaatttt tataaatata gttgaatagc atctagcctt atggcatcat taatgatgta
     3961 aagattaatt aggaggggat tctcttggca gtaccaaatg aattgaaata ttcaaaagag
     4021 catgaatggg ttaaagttga aggtaatgta gcaacaattg gaatcacaga atacgcacaa
     4081 agcgagttag gtgatattgt tttcgttgaa ttaccggaaa cagatgatga aattaatgaa
     4141 ggggatacgt ttggtagcgt agaatcagtt aaaactgtat cagaattata tgcaccaatc
     4201 tctggtaaag tagttgaagt caacgaagaa ctagaagata gtcccgaatt tgtaaatgaa
     4261 tctccatacg aaaaagcatg gatggtaaaa gtagaaatta gtgatgaaag tcagcttgaa
     4321 gctttattaa cagctgaaaa atattcagaa atgattggtg aataatcacc gtgtaactcc
     4381 ttaatctaag attgaggagt ttatttttag tctgagtaag taaataatat gaaagaaaat
     4441 ttattaggta ctattatttg gagtattgct acattttatt attcaagaat gatgaaaata
     4501 atgaatttag ctattttaaa aataaaaatt gggggaagtt aatatgctaa acattcaaga
     4561 cgttagtcat ctttctaaaa aggagcaaaa agcatataac cgtttcgtag aatctgtaga
     4621 aaacggtaat ttaccagtac taccatgtat tgaaatggat ctaaaagaga tgaaagaaga
     4681 aacattaaac cagagtaaga ttggtggaat gccattttta aaatctttta aagatatacc
     4741 attagatgaa aataatgtac caatggtatt gttagcgcag attaatttgg atgatcttcc
     4801 agaacaacaa gaattatttc ctgtaaaaga agggatattg cagttttgga ttagttcaga
     4861 agatcaaatg tatggaatgt ctgaaaattt aaagggaaac aatataaact caaggcttgt
     4921 ttatataaaa gagccaatta cagatttatc actcgaaaat attcaagcgc atttgaagtc
     4981 attagatgct gataatgagg atatcccgtt cagtggagca ttttctatag aatttagatt
     5041 gtcgaaacaa actattacat gtactgatta taagtacgat gaggacgtgc ttgcattgtg
     5101 gaataaagtc aatccatcct tcgcgctaaa atcaatgttt ggtggttatg atgaattgat
     5161 ggaacctgtg tgtaacacat ttactgctaa ggaaccattt aatcaacttg gtggttatcc
     5221 atattttgac caaatagatc caagaacgaa cgatcaagaa ctgaaaatgt atgatagagt
     5281 cttactgcaa attgattcta caagagatgg taattcttcg attatatggg gtgatttagg
     5341 tattgccaat atcttagtga aatctactga ccttgaggct atgaagtttg atgattacat
     5401 gtattcatgg gattgcagct aagagaatca tgaaaatata ttactttaat aagtgtagta
     5461 tctatagatc aatctaggga gtgggacaga aatgaaaaag aaccactagt gatttataat
     5521 gtagtggttc tttttcataa gccacagcta atgtgtactt aaaaatagga atacatgagt
     5581 aaaactcatg cataagaaat actaatttct aaagaaaaag tatttcttta tgttggggcc
     5641 ccgctataat tgaaaagttt gttataggtg tattttcttt tggttaacta ttggtaatat
     5701 aacattgtag attttaggat gttgattttt gacttagcct ttgtctgctt atgttaaatg
     5761 tcccagctag tcaaacattg attttgtaat tattatatta agctagttta aattttttgt
     5821 gatttatgtt ttattcatac caattttgtg aaatgataca gtgacagatg tttattgatt
     5881 ttaaagggtg atgatgatgg ctattgtaaa taaagtgata attgttgaag gaaaatctga
     5941 taaaaaaagg gtgcaacagg ttattgcaga accagtcaat attatttgta ctcatggaac
     6001 aatgagtata gataagcttg atgatatgat agaatcactg tatgataaac aagtttttgt
     6061 attagccgat tctgatgacg aaggagatcg aattagaaat tggtttaaac gttatttgag
     6121 tgaaagtgaa catatattta ttgataaaac ttactgtcaa gttgcgaatt gccccaaaca
     6181 atatttggcg catgtacttt caaaacatgg ctttacttgt aagaaagaaa cacctctttt
     6241 accgaatata aataatgaaa ggttagtttt agtaaatgaa taattcatta gacatcaaag
     6301 atgtaactac attttatgag gaagacaaac atttaatctt tggttataca ccaacgtgtg
     6361 gtacttgtaa ggtttcagaa agaatgttag acattgctaa tgaaatattg cagttgccat
     6421 tattgaaaat agatttaaac ttttatcctc agttttgtaa agatatgcaa atcatgtcta
     6481 cgccgatttt attgttgatg aataaagata aagaagtaaa acgaatttat gcatttaaat
     6541 cggtgactga tttgttagaa aatttaaaat agttattgac gaaattttac taaggtgtta
     6601 ggattaatac aaattaaatg atgcgtaaac tcttatcgag agtggtggag ggatgtgccc
     6661 tacgaagccc ggcaaccgtc ttatatagaa atggtgccaa ttcacataaa gttttaactt
     6721 ttgaagatga gagaaacaat actactattg ctttctcaat ttttctatcg atattgagaa
     6781 agcatttttt tattttatta agcaacacag ggaggaatca acgtgattga attaaaagaa
     6841 gttgttaaag aatatcggac taaaaataaa gaagtccttg ctgtagatca cgttaattta
     6901 tcgattcgag caggatcgat ttatggcgtc attggttttt ctggagcagg aaaaagtact
     6961 ttgattcgaa tgtttaatca tttagaagcg cctacatcag gtgaagttat tatagatggg
     7021 gaccatatag gtcaattgcc caaaaatgga ttaagagcaa aaagacaaaa agtaagtatg
     7081 atcttccaac attttaattt attatggtca aggactgtgt taaaaaatat tatgtttccg
     7141 cttgaaattg caggtgtccc tagaaggaga gctaagcaaa aagcattaga acttgtcgaa
     7201 cttgtcggtt taaaaggtag agaaaaagct tatccatcag agttatcagg tggacaaaag
     7261 caacgtgttg ggattgcacg agcgttagct aatgatccaa cggtcttgct ttgtgatgag
     7321 gcaacaagtg cacttgatcc gcaaacaaca gatgaaattt tagatctact actaaaaatt
     7381 agagaacaac aaaatttaac aattgtacta attacgcatg aaatgcatgt cattcgtcgt
     7441 atttgtgatg aagttgcagt tatggaaagt ggtaaagtga tagaacaagg accggtgaca
     7501 caggtttttg aaaatccgca acacactgtg acaaaacgat ttgtgaaaga cgatttaaat
     7561 gatgatttcg aaacatcttt aacagaatta gagccattag aaaaagatgc ttatatcgtt
     7621 agattagttt tcgctggttc aacaacaacc gagcctattg tatcgagcct atcaactgcc
     7681 tatgatatta aaattaatat tttagaagca aatattaaaa atacaaaaaa tggaacagtc
     7741 ggctttttag ttctgcatat tccatatatt tcaagtgtag atttcggaaa attcgaaaaa
     7801 gagttaattg agcgacaagt taaaatggag gtgttaagac atgggtaaat catttagtga
     7861 aattataaat gaaatgatta caatgcctaa tattcagtgg ccagaagttt ggactgcaat
     7921 agtcgaaaca ctatacatga cagtcgtctc aactatattt gcatttatac ttggtcttat
     7981 tttaggtgtg ttattattct tgtctgctaa aggtaagtct atcggtgcaa ggttatttta
     8041 ttctatcgtt tctttcattg ttaacttatt tagagcgata ccatttatta ttttaatttt
     8101 attattaatt ccatttacaa gtttgatact tggaacgata agtggtccga caggtgcgtt
     8161 accagccttg atcattggcg cagcaccgtt ttatgcaagg ctcgtagaaa ttgcttttaa
     8221 agaaattgat aaaggcgtca tcgaagcggc ttggtcaatg ggtgctaata cttggacagt
     8281 aattcgtaaa gtccttttac ctgaagccat gccagcgcta gtgtctggca ttacagttac
     8341 agcaatcgct ttagttggtt caacagcagt tgcaggtgta attggtgccg gtggtttagg
     8401 aaatttagca tacttaacag gtttcactcg aaatcaaaat gatgtcattt tagtatcaac
     8461 agtttttatt ttaattattg tatttataat ccaattcatt ggggattggc ttacaaataa
     8521 acttgataaa cgataaattg ggggtttcat tttatgaaaa aattatttgg tcttatttta
     8581 gtattaacat ttgcagttgt attagcagct tgcggtaatg gaaacaaaag tggcagtgac
     8641 gacaagaaaa taacagtagg tgcttcacca gcaccacatg ctgaaatttt agaaaaagca
     8701 aaaccattat tagagaaaaa aggttatgaa ctagatatta aaacaattaa cgattacact
     8761 acgcctaata aattactaga caaaggtgaa attgacgcaa actatttcca acatacacca
     8821 tatttaaaca cagagaaaaa ggataaaggt tacaaaatcg taagtgccgg tgatgttcac
     8881 ttagaaccta tggctgtata ctctaaaaag tataaaagtt taaaagaatt accaaaaggt
     8941 gcaacagttt atgtgtctaa taatccagct gaacaaggac gcttcttaaa attcttcgtt
     9001 gatgcaggtt taattaagat caaaaaaggc gtaaaaattg aagatgctaa gtttagtgat
     9061 attacagaga ataaaaaaga tattaagttt aataataaac aatcagcaga attcttacct
     9121 aaaatatatc aaaatgaaga cgcggatgct gttatcatta attcaaactt tgcaatcgaa
     9181 caaaaactaa atcctaaaaa agattctatt gctgtagaaa gtgcgaaaga taatccttat
     9241 gcaaacttaa ttgctgttaa agaaggaaat caagatgata agaaaatcaa agcattaatt
     9301 gaagtattac aatctaaaga tattcaagac ttcattaatg aaaaatacaa tggtgcagtt
     9361 attcctgcta aataattatt aatggctcag taacgtgatg ttgctgggct ttttaattta
     9421 aacaggtatt tatatgatat ttaggaatga gatgctatta cggataaagt aaaatcctat
     9481 atgcatgcgt tctactcatt tattcctatt tttaatttca catggatagt gactactaca
     9541 tcataaatca ttagtgattc tttatcattt ctgtcccact ccctcaagtt aatgatgaat
     9601 ctatttcgct agttataaaa tgatgtcaaa tcaataacaa aaattattta agtaaaatgt
     9661 ttagataatt tttcagtggg taagtattat atataacatt taattattcc gaggaggcat
     9721 ttattatggc agacgaaagt aaattcgaac aagcaaaagg taatgttaaa gaaacagtag
     9781 gtaatgttac tgataataaa aatttagaaa acgaaggtaa agaagataaa gcttctggta
     9841 aagcgaaaga attcgttgaa aatgcaaaag aaaaagcaac tgattttatt gataaagtaa
     9901 aaggtaacaa aggcgagtaa ttttactttt ttattcctaa tacactgcac atgccactta
     9961 gtcggtataa ccatcggcta ggtggttttt gtattaaaaa agtggatacc aaaatttatt
    10021 aataattatt ttaatgttag aaaaaaacta aataaaaact cgctaatgat atccaataat
    10081 atgtatacaa aacgagacat atattgcata tgattaacga gatactgaaa atattttatc
    10141 acccctaaaa tgactattca ttttcagcgg taattcgacc taaagtcaaa cttacaataa
    10201 aaccgatgat aaatactact aatgaaacaa accacatcac gatattagtt ggtaaacctg
    10261 gaaatactgc aaagagggag ccaacaacaa aaccaatgat taatgcaaaa gtcattagtt
    10321 tatgatgtgt taggaaatac tggataattt tgcttgaaat aatgaatcca gcaagcacgc
    10381 caaatccgac tgcaagtaat ataggaagac ctgtaaagtt aagtttaaca acttcagata
    10441 ttgctagcat gactgtgcca tagacgccaa atactaataa cataaatgaa cctgaaatac
    10501 ctgggagtaa catagcacta gatgcacaca tacctgcaat aaagtattta ataataagac
    10561 cagttgatag agtaagtgtt tctccagcat gtttatcgcc attattcatt aatgtaataa
    10621 caattaagat agcgatacca gctataacca tcatgtaatg tttagttgta aatgacgttt
    10681 tatagttaga aattttcaat aaatatggaa cgataccaat gattaatcca ccaaagaaaa
    10741 acatagttgg aatatggtgt tggcttaata aataattaaa aagattactt agtgatccca
    10801 ttgccagtaa cattccaatt ataatgggga ttaaaaatgt aaaacttggc caaaaacgtc
    10861 gtgagaatat gccgctaatt gaagcgataa attgattgta tatacctaac aataatgcaa
    10921 tagtcccacc gctaacacca ggcaccaagt cactcgttcc catagcaaaa ccttttagaa
    10981 tattaatcca tttaaattgt tgcatgaata actcctttca aacgattgga ataaaatcat
    11041 aaatagcatc ataccatatt acaaatgtcc tagtgaaatg ataacatatt ttaaattcat
    11101 aaaatccatt gagaaattat gtgcacttat tatcatttat attt
//
LOCUS       DAHHOJ010000088        11123 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.088, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000088 DAHHOJ010000000
VERSION     DAHHOJ010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11123)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11123)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11123
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.088"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(236..1129)
                     /locus_tag="NBH32_002001"
     CDS             complement(236..1129)
                     /locus_tag="NBH32_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1515583.1"
                     /translation="MKKTILLTMTTLTLFSMSPNSAQAYTNDSKTLEEAKKAHPNAQF
                     KVNKDTGAYTYTYDKNNAPNNNHQNQSRTNDNHQHANQRDFNNNQYHSSLSGQYTHIN
                     DAIDSHTPPQTSPSNPLTPAIPNVEDNDDELNNAFSKDNKGLITGIDLDELYDELQIA
                     EFNDKAKTADGKPLALGNGKIIDQPLITSKNNLYTAGQCTWYVFDKRAKDGHTISTFW
                     GDAKNWAGQASSNGFKVDRHPTRGSILQTVNGPFGHVAYVEKVNIDGSILISEMNWIG
                     EYIVSSRTISASEVSSYSYIH"
     gene            1377..1670
                     /gene="esxA"
                     /locus_tag="NBH32_002002"
     CDS             1377..1670
                     /gene="esxA"
                     /locus_tag="NBH32_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion effector EsxA"
                     /protein_id="HCD1515584.1"
                     /translation="MAMIKMSPEEIRAKSQSYGQGSDQIRQILSDLTRAQGEISANWE
                     GQAFSRFEEQFQQLSPKVEKFAQLLEEIKQQLNSTADAVQEQDQQLSNNFGLQ"
     gene            1753..4782
                     /gene="esaA"
                     /locus_tag="NBH32_002003"
     CDS             1753..4782
                     /gene="esaA"
                     /locus_tag="NBH32_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaA"
                     /protein_id="HCD1515585.1"
                     /translation="MKKKNWIYALIVTLIIIIAIVSMIFFVQTKYGDQSEKGSQSVSN
                     KNNKIHIAIVNEDQPTTYNGKKVELGQAFIKRLANEKNYKFETVTRNVAESGLKNGGY
                     QVMIVIPENFSKLAMQLDAKTPSKISLQYKTAVGQKEEVAKNTEKVVSNVLNDFNKNL
                     VEIYLTSIIDNLHNAQKNVGAIMTREHGVNSKFSNYLLNPINDFPELFTDTLVNSISA
                     NKDITKWFQTYNKSLLSANSDTFRVNTDYNVSTLIEKQNSLFDEHNTAMDKMLQDYKS
                     QKDSVELDNYINALKQMDSQIDQQSSMQDTGKEEYKQTVKENLDKLREIIQSQESPFS
                     KGMIEDYRKQLTESLQDELANNKDLQDALNSIKMNNAQFAENLEKQLHDDIVKEPDTD
                     TTFIYNMSKQDFIAAGLNEDEANKYEAIVKEAKRYKNEYNLKKPLAEHINLTDYDNQV
                     AQDTSSLINDGVKVQRTETIKSNDINQLTVATDPHFNFEGDIKINGKKYDIKDQSVQL
                     DTSNKEYKVEVNGVAKLKKDAEKDFLKDKTMHLQLLFGQANRQDEPNDKKATSVVDVT
                     LNHNLDGRLSKDALSQQLSALSRFDAHYKMYTDTKGREDKPFDNKRLIDMMVDQVIND
                     MESFKDDKVAVLHQIDSMEENSDKLIDDILNNKKNTTKNKEDISKLIDQLENVKKTFA
                     EEPQEPKIDKGKNDEFNTMSSNLDKEISRISEKSTQLLSDTQESKSIADSVSGQLNQV
                     DNNVNKLHATGRALGVRANDLNRQMAKNDKENDLFAKEFKKVLQNSKDGDRQNQALKA
                     FMSNPVQKKNLENVLANNGNTDVISPTLFVLLMYLLSMITAYIFYSYERAKGQMNFIK
                     DDYSSKNHLWNNVITSGVIGTTGLIEGLIVGLIAMNKFHVLAGYRAKFILMVILTMMV
                     FVLINTYLLRQVKSIGMFLMIAALGLYFVAMNNLKAAGQGVTNKISPLSYIDNMFFNY
                     LNAEHPIGLALVILTVLVIIGFVLNMFVKHFKKERLI"
     gene            4782..5240
                     /gene="essA"
                     /locus_tag="NBH32_002004"
     CDS             4782..5240
                     /gene="essA"
                     /locus_tag="NBH32_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssA"
                     /protein_id="HCD1515586.1"
                     /translation="MLMNSVIALTFLTASSNNGGLNIDVQQEEEKRINNDLNQYDTTL
                     FNKDSKAVNDAIAKQKKERQQQIKNDMFQNQASHSTRLNETKKVLFSKSNLEKTSESD
                     KSPYIQNKQEKKIFPYILMSVGAFLTLGFVIFSIHKGRRTKNESARKSNI"
     gene            5212..5454
                     /gene="esaB"
                     /locus_tag="NBH32_002005"
     CDS             5212..5454
                     /gene="esaB"
                     /locus_tag="NBH32_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001071606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EsaB"
                     /protein_id="HCD1515587.1"
                     /translation="MNQHVKVTFDFTNYNYGTYDLAVPAYLPIKNLIALVLDSLDISI
                     FDVNTQIKVMTKGQLLVENDRLIDYQIADGDILKLL"
     gene            5467..6801
                     /gene="essB"
                     /locus_tag="NBH32_002006"
     CDS             5467..6801
                     /gene="essB"
                     /locus_tag="NBH32_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssB"
                     /protein_id="HCD1515588.1"
                     /translation="MVKNHNPKNEMQDMLTPLDAEEAAKTKLRLDMREIPKSSIKPEH
                     FHLMYLLEQHSPYFIDAELTELRDSFQIHYDINDNHTPFDNIKSFTKNEKLRYLLNIK
                     NLEEVNRTRYTFVLAPDELFFTRDGLPIAKTRGLQNVVDPLPMSEAEFLTRYKALVIC
                     AFNEKQSFDALVEGNLELHKGTPFETKVIEAATLDLLTSFLDEQYQKQEQDYSENYAY
                     VRKVGHTVFKWVAIGMTTLSVLLIAFLAFLYFSVMKHNERIEKGYQAFVKDDYTQVLN
                     TYDDLDGKKLDKEALYIYAKSYIQTNKQGLEKDKKENLLNNVTPNSNKDYLLYWMELG
                     QGHLDEAIDIATYLDDNDITKLALINKLNEIKNNGDLSNDKRSEETKKYNDKLQDILD
                     KEKQVKDEKAKSEEEKAKAKDEKLKQQEENEKKQKEQAQKDKEKRQEAERKK"
     gene            6823..>11123
                     /gene="essC"
                     /locus_tag="NBH32_002007"
     CDS             6823..>11123
                     /gene="essC"
                     /locus_tag="NBH32_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VII secretion protein EssC"
                     /protein_id="HCD1515589.1"
                     /translation="MHKLIIKYNKQLKMLNLRDGKTYTISEDERADITLKSLGEVIHL
                     EQNNQGTWQANHTSINKVLVRKGDLDDITLQLYTEADYASFAYPSIQDTMTIGPNAYD
                     DMVIQSLMNAIIIKDFQSIQESQYVRIVHDKNTDVYINYELQEQLTNKAYIGDHIYVE
                     GIWLEVQADGLNVLSQNTVASSLIRLTQEMPHAQADDYNTYHRSPRIIHREPTDDIKI
                     ERPPQPIQKNNTVIWRSIIPPLVMIALTVVIFLVRPIGIYILMMIGMSTVTIVFGITT
                     YFSEKKKYNKDVEKREKDYKAYLDNKSKEINKAIKAQRFSLNYHYPTVAEIKDIVETK
                     APRIYEKTSHHHDFLYYKLGIANVEKSFKLDYQEEEFNQRRDELFDDAKELYEFYTDV
                     EQAPLINDLNHGPIAYIGARHLILEELEKMLIQLSTFHSYHDLEFLFVTREDEVETLK
                     WARWLPHMTLRGQNIRGFVYNQRTRDQILTSIYSMIKERIQAVRERSRSNEQIIFTPQ
                     LVFVITDMSLIIDHVILEYVNQDLSEYGISLIFVEDVIESLPEHVDTIIDIKSRTEGE
                     LITKEKELVQLKFTPENIDNVDKEYIARRLANLIHVEHLKNAIPDSITFLEMYNVKEV
                     DQLDVVNRWRQNETYKTMAVPLGVRGKDDILSLNLHEKAHGPHGLVAGTTGSGKSEII
                     QSYILSLAINFHPHEVAFLLIDYKGGGMANLFKDLVHLVGTITNLDGDEAMRALTSIK
                     AELRKRQRLFGEHDVNHINQYHKLFKEGIATEPMPHLFIISDEFAELKSEQPDFMKEL
                     VSTARIGRSLGIHLILATQKPSGVVDDQIWSNSKFKLALKVQDRQDSNEILKTPDAAD
                     ITLPGRAYLQVGNNEIYELFQSAWSGATYNIEGDKLEVEDKTIYMINDYGQLQAINKD
                     LSGLEDEETKENQTELEAVIDHIESITTRLEIEEVKRPWLPPLPENVYQEDLVETDFR
                     KLWSDDAKEVELTLGLKDVPEEQYQGPMVLQLKKAGHIALIGSPGYGRTTFLHNIIFD
                     VARHHRPDQAHMYLFDFGTNGLMPVTDIPHVADYFTVDQEDKIAKAIRKIHDIISERK
                     RLLSQERVVNIEQYNKETGNSIPNIFLIIDNYDTVKESPFMEEYEEMMSKVTREGLAL
                     GVYIILSGSRSSAIKSAIFTNIKTRVALYLFENNELTNIIGSYKKGVKDVKGRAAIND
                     DNFTQFQIAQPFELAEGQTYNERIKNEVAQMKEFYVGDYPKHIPMMPDKVLMDDIQET
                     YDLEKIIHEEHKLPLGLDFEDVELVGFDLSQTNIFTSVKPVDIDNGLTLLEKQLNIIS
                     NEYEIATLDTKGILKNTGYEDYLYCGDKKEIISFKNELVSFIKNVEPRKKWIVVISDF
                     KEFINIASPNNDEIKTIFLDGPKNNVFPIIYGLYQETIGGFSSQIKLLKEIVSSA"
BASE COUNT         4269 a         1578 c         1985 g         3291 t
ORIGIN      
        1 tagttataat aatgtttaat aagttattga aaatctaata aactacaaaa aatagtttga
       61 ttacataatg attcttgaaa aatgtcggtt aactttaagg tgacaagtta ttgcaatttt
      121 gaaggtaaac atcatatttt ataatcgaat aacatacaaa ataaagcgac caattttgca
      181 gtgttacgaa ttgtaaactg cgaactggtc gctttttatt gatgtcatga ttaatttaat
      241 ggatgtaact atatgatgaa acttctgaag cagagatggt tcttgatgaa acgatatatt
      301 caccaatcca attcatttct gaaattagaa tacttccatc aatattaact ttctcaacgt
      361 aggctacatg accaaatgga ccgtttactg tttgcaaaat tgatcctcgt gttgggtgtc
      421 tatctacttt gaagccattg cttgaagctt ggcctgccca gtttttagca tctccccaaa
      481 atgtactaat cgtgtgtcca tctttggcac gtttatcaaa gacataccat gtacattgtc
      541 cagcagtata taagttgttc ttacttgtga taagaggctg atcaatgatt ttaccgttac
      601 ctaatgctaa aggtttaccg tcagcagtct ttgctttgtc attaaattcg gcgatttgta
      661 attcgtcata caattcgtct aaatcgatgc ctgtaataag ccctttgtta tcttttgaaa
      721 aagcgttatt taattcatcg tcattgtctt cgacattcgg tatggctggt gtcaaaggat
      781 tgcttggtga cgtttgaggc ggtgtgtgtg aatcaattgc gtcattaatg tgcgtatact
      841 gaccacttaa tgaagaatgg tactgattgt tgttaaaatc acgttgattt gcgtgttgat
      901 gattgtcgtt tgtacgtgac tgattttgat gattgttgtt tggcgcgttg tttttgtcat
      961 aagtataagt atacgcgccg gtgtctttat tcactttgaa ctgtgcgttt gggtgtgctt
     1021 tctttgcttc ttctaatgtt ttgctatcat tcgtgtatgc ttgagccgag ttaggcgaca
     1081 tactaaataa agtaagagtt gtcatcgtca gtaaaattgt tttcttcata ataaccattt
     1141 aatcctttat gtatttaatt taattttagt atacacattt atattacaaa aatgaatggt
     1201 taattaaaaa tatatgggta tattcaatat atttatttaa aaaaagctaa aaatacttaa
     1261 aaaactatat acatatacta ataatttata tattatttga gtaaggagca ctttttcaaa
     1321 aaatagtgtc cctaaaaagt tttgataaac ttaaaatatt caggaggttt ctagttatgg
     1381 caatgattaa gatgagtcca gaggaaatca gagcaaaatc gcaatcttac gggcaaggtt
     1441 cagaccaaat ccgtcaaatt ttatctgatt taacacgtgc acaaggtgaa atttcagcga
     1501 actgggaagg tcaagctttc agccgtttcg aagagcaatt ccaacaactt agtcctaaag
     1561 tagaaaaatt tgcacaatta ttagaagaaa ttaaacaaca attgaatagc actgctgatg
     1621 ccgttcaaga gcaagaccaa caactttcta ataatttcgg tttgcaataa gcattctgaa
     1681 attggcaaag tcacattttc taatgtggct ttgcttatca ttttttaaga aaacaactga
     1741 aaggaaataa gcatgaaaaa gaaaaattgg atttatgcat taattgtcac tttaattatt
     1801 ataattgcca tagttagtat gatatttttt gttcaaacaa aatatggaga tcaatcagaa
     1861 aaaggatccc aaagtgtaag taataaaaat aataaaatac atatcgcaat tgttaacgag
     1921 gatcaaccaa cgacatataa cggtaaaaag gttgagctgg gtcaagcatt tattaaaagg
     1981 ttagcaaatg agaaaaacta taaatttgaa acagtaacac gaaacgttgc tgagtctggt
     2041 ttgaaaaatg gtggatacca agtcatgatt gttatcccag aaaacttttc aaaattggca
     2101 atgcaattag acgctaaaac accatcgaaa atatcactac agtataaaac agctgtagga
     2161 caaaaagaag aagtagctaa aaacacagaa aaagttgtaa gtaatgtact taatgacttt
     2221 aacaaaaact tggtcgaaat ttatttaaca agcatcattg ataatttaca taatgcacaa
     2281 aaaaatgttg gcgctattat gacgcgtgaa catggtgtga atagtaaatt ctcgaattac
     2341 ttattaaatc caattaacga cttcccggaa ttatttacag atacgcttgt aaattcaatt
     2401 tctgcaaaca aagatattac aaaatggttc caaacataca ataaatcatt attgagtgcg
     2461 aattcagata cgttcagagt gaacacagat tataatgttt cgactttaat tgaaaaacaa
     2521 aattcattat ttgacgagca caatacagcg atggataaaa tgctacaaga ttataaatcg
     2581 caaaaagata gcgtggaact tgataactat atcaatgcat taaaacagat ggacagccaa
     2641 attgatcaac aatcaagtat gcaagataca ggtaaagaag aatataaaca aactgttaaa
     2701 gaaaacttag ataaattaag agaaatcatt caatcacaag agtcaccatt ttcaaaaggt
     2761 atgattgaag actatcgtaa gcaattaaca gaatcactgc aagatgagct tgcaaataac
     2821 aaagacttac aagatgcgct aaatagcatt aaaatgaaca atgctcaatt cgctgaaaac
     2881 ttagaaaaac aacttcatga tgatattgtc aaagaacctg atacagatac aacatttatc
     2941 tataacatgt ctaaacaaga ctttatagct gcaggtttaa atgaggatga agctaataaa
     3001 tacgaagcaa ttgtcaaaga agcaaaacgt tataaaaacg aatataattt gaaaaaaccg
     3061 ttagcagaac acattaattt aacagattac gataaccaag ttgcgcaaga cacaagtagt
     3121 ttgattaatg atggtgtcaa agtgcaacgt actgaaacga ttaaaagtaa tgatattaat
     3181 caattaactg ttgcaacaga tcctcatttt aattttgaag gcgacattaa aattaatggt
     3241 aaaaaatatg acattaagga tcaaagtgtt caactcgata catctaacaa ggaatataaa
     3301 gttgaagtca atggcgttgc taaattgaaa aaggatgctg agaaagattt cttaaaagat
     3361 aaaacaatgc atttacaatt gttatttgga caagcaaatc gtcaagatga accaaatgat
     3421 aagaaagcaa cgagtgttgt ggatgtaaca ttgaatcata accttgatgg tcgcttatcg
     3481 aaagatgcat taagccagca attgagtgca ttatctagat ttgatgcaca ttataaaatg
     3541 tacacagata caaaaggcag agaagataaa ccattcgata acaaacgttt aattgatatg
     3601 atggttgacc aagttatcaa tgacatggaa agtttcaaag acgataaagt agctgtgtta
     3661 catcaaattg attcaatgga agaaaactca gacaaactga ttgatgacat tttaaataac
     3721 aaaaagaata caaccaaaaa taaagaagat atttctaagc tgattgatca gttagaaaac
     3781 gttaaaaaga catttgctga agagccacaa gaaccaaaaa ttgataaagg caaaaatgat
     3841 gaatttaata cgatgtcttc aaatttagat aaagaaatta gtagaatttc tgaaaagagt
     3901 acgcaattgc tatcagatac acaagaatca aaatcaattg cagattctgt tagtggccaa
     3961 ttaaatcaag tcgacaataa tgttaataag ctacatgcga caggtcgagc attaggcgta
     4021 agagcgaatg acttgaaccg tcaaatggct aaaaacgata aagaaaatga tttattcgct
     4081 aaagagttta aaaaagtatt acaaaattct aaagatggcg acagacaaaa ccaagcatta
     4141 aaagcattta tgagtaatcc ggttcaaaag aaaaacttag aaaatgtttt agctaataat
     4201 ggtaatacag acgtgatttc accgacatta ttcgtattat tgatgtactt actatcaatg
     4261 attacagcat atattttcta tagttatgaa cgtgccaaag gacaaatgaa tttcattaaa
     4321 gatgattata gtagtaaaaa tcatctttgg aataatgtca ttacgtcagg tgttattggt
     4381 acaactggtt tgatagaagg attaattgtc ggtttaattg caatgaataa gttccatgta
     4441 ttagctggct atagagcgaa attcatctta atggtgattt taactatgat ggtcttcgta
     4501 cttattaata cgtatttact aagacaggta aaatctatcg gtatgttctt aatgattgct
     4561 gcattgggtc tatactttgt agctatgaat aatttgaaag cagctggaca aggtgtgact
     4621 aataaaattt caccattatc ttatatcgat aacatgttct tcaattattt aaatgcagag
     4681 catcctatag gcttggcgct agtaatatta acagtacttg tgattattgg cttcgtactg
     4741 aacatgtttg taaaacactt taagaaagag agattaatct aatgttgatg aatagcgtga
     4801 ttgctttaac ttttttaaca gcatctagca ataatggcgg acttaatatt gacgtgcaac
     4861 aagaagagga aaagcgaatc aataatgatt taaatcaata tgatacaacg ctatttaata
     4921 aagacagcaa agcggttaat gatgcgattg ctaagcagaa aaaagaaaga caacaacaaa
     4981 taaaaaatga tatgtttcaa aatcaagcga gtcactcgac tcgcttgaat gaaactaaaa
     5041 aagtgttatt ttccaaatct aacttagaaa agacttcgga gagtgataaa agcccctata
     5101 ttcaaaacaa gcaggagaaa aaaatattcc cgtacatttt gatgtctgta ggggcttttt
     5161 tgactttagg atttgtcatt ttttcaattc ataaagggag acgaacgaaa aatgaatcag
     5221 cacgtaaaag taacatttga ttttactaat tataattacg gcacatatga cttagcagta
     5281 ccagcatatt taccgattaa gaatctaata gcattagtgt tagacagttt ggatatttcg
     5341 attttcgatg tgaatacaca aattaaagtg atgacgaaag gtcaattact tgttgaaaac
     5401 gaccgactta ttgattatca aatcgctgat ggagatattt tgaagttact ataggaggaa
     5461 aaatagatgg ttaaaaatca taaccctaaa aatgaaatgc aagatatgtt aacaccttta
     5521 gatgccgaag aagcagctaa aacaaaatta cgtttagata tgagagagat tcctaagtct
     5581 tcaattaaac cagaacattt tcatttaatg tacttattag aacaacattc tccatatttt
     5641 atagatgctg aactaactga actacgtgat agtttccaaa tacattatga cattaatgac
     5701 aatcatacac cttttgataa tattaaatca tttactaaaa atgaaaaatt acgttactta
     5761 cttaatatca aaaatttaga agaagtaaat cgtacacgct acacatttgt gttggcacca
     5821 gatgaattat ttttcacaag agatggattg ccgattgcca aaacgagagg attacaaaat
     5881 gttgttgacc ctttgccaat gtcggaagct gaatttttaa caagatataa agcactggtt
     5941 atatgtgcat tcaatgagaa acaatcattt gacgcattag ttgaaggaaa cttagaacta
     6001 cataaaggaa caccatttga aactaaagtt attgaagcgg caacgttaga cttattaact
     6061 tcgtttttag atgaacaata tcagaaacaa gaacaagatt atagtgaaaa ttatgcatat
     6121 gttcgtaaag tagggcatac cgtttttaaa tgggtcgcta tcggtatgac aacgttaagt
     6181 gtgttattaa ttgcattttt agctttttta tatttttcag taatgaagca taatgagcgc
     6241 attgaaaaag gataccaagc atttgtaaag gatgattata cgcaagtact aaatacgtat
     6301 gatgatttag atggtaaaaa attagacaaa gaagcgcttt acatttatgc caaaagctat
     6361 atccaaacga acaaacaagg tttagaaaaa gataagaaag aaaacttgct taataatgtg
     6421 acgccaaatt cgaacaaaga ttacttatta tattggatgg aattaggaca aggacatctt
     6481 gatgaagcga ttgacattgc aacatattta gatgataacg atattacgaa attagcgttg
     6541 attaataaat taaatgagat taaaaataat ggtgatttat cgaatgataa acgttctgaa
     6601 gaaactaaaa aatataacga taaattgcaa gatattttag ataaagaaaa gcaagttaaa
     6661 gatgaaaaag caaaatctga agaagagaaa gcaaaagcga aagatgagaa attaaagcaa
     6721 caagaagaga acgaaaagaa acaaaaagaa caagcacaaa aagataaaga aaaacgtcaa
     6781 gaagctgaaa gaaaaaaata gtataggact gaggcaaaga caatgcataa attgattata
     6841 aaatataaca aacaattgaa gatgctcaat ttgcgagatg gtaagacata tactattagc
     6901 gaagacgagc gtgcagatat tacgttgaaa tcgttaggcg aagtcattca tttagaacaa
     6961 aataatcaag gtacttggca agcgaatcat acttctatta ataaggtgct tgttagaaaa
     7021 ggtgaccttg atgacattac attacaactt tatacagaag ctgattatgc atcatttgct
     7081 tatccttcaa ttcaagatac gatgacaatt gggccaaatg cgtatgatga tatggttatt
     7141 caaagcttga tgaatgccat cattattaaa gattttcaat caatacaaga atcacaatac
     7201 gtacgcattg tgcacgataa aaatacagat gtgtatatta actatgaact acaagagcaa
     7261 ttaacaaaca aagcttacat tggtgatcat atttatgttg aagggatatg gctcgaagta
     7321 caagctgatg gtttaaatgt attgagtcag aatacagtgg catcgtcatt aattcgctta
     7381 acacaagaga tgccacatgc acaggcagat gattacaata cgtaccatcg ttcgccaagg
     7441 attattcacc gtgaaccgac cgatgatatt aagattgaaa gaccaccaca gccaatacag
     7501 aagaacaata cagtgatatg gcgttccatt ataccgccat tagtaatgat tgctttaact
     7561 gttgtcatct ttttagtgag accaattggt atttatattt taatgatgat tggtatgagt
     7621 acagtaacga tagtatttgg tattacaacg tatttctctg aaaagaaaaa gtataacaaa
     7681 gatgttgaaa aacgagagaa agattacaaa gcttatttgg ataataaatc taaagaaatt
     7741 aataaagcga ttaaagcaca acgttttagt ttgaattatc attatccaac ggttgctgaa
     7801 attaaagata tcgttgaaac gaaagcacca agaatatatg aaaaaacatc gcatcatcac
     7861 gatttcttat attataagtt aggtattgcg aatgtagaaa agtcattcaa attagattac
     7921 caagaagaag aatttaacca acgtcgtgat gaactattcg acgatgctaa agaattgtat
     7981 gaattttaca cagatgtaga acaagcacca ttaatcaatg atttaaatca tgggccaatt
     8041 gcatatattg gtgcacgaca tctcatttta gaagaattgg agaaaatgct aatccaattg
     8101 tcaacgttcc atagttatca tgatttagag tttctatttg tgacacgtga agatgaagtt
     8161 gaaacattga aatgggcacg ttggttgcca catatgacat tgagaggtca aaacattaga
     8221 gggtttgttt acaatcaacg aacacgtgac caaattttaa cgtcaattta tagcatgatt
     8281 aaagaacgta tccaagctgt gcgtgaacgc agcagaagta atgagcaaat tattttcaca
     8341 ccgcaattag tgtttgtcat tacagatatg tcattaatta ttgatcacgt cattttagaa
     8401 tatgtaaacc aagatttatc agaatatggt atttcattaa tctttgttga agatgtgatt
     8461 gaaagtttgc cagagcatgt agataccatt attgatatca agtctcgtac tgaaggcgaa
     8521 ctgattacga aagaaaaaga attagttcaa ttgaaattta cacctgaaaa tattgataac
     8581 gtcgataaag aatatatcgc gcgacgtttg gcgaatttga tacacgtcga acatttgaaa
     8641 aatgcaattc ctgatagtat tacattttta gaaatgtata acgtgaaaga agtagatcag
     8701 cttgatgtgg ttaatcgatg gagacaaaac gaaacataca aaacgatggc agtaccttta
     8761 ggtgtaagag gtaaagatga cattttatca ttgaacttac atgaaaaagc acacggacca
     8821 catggtttag ttgctggtac cactggttca gggaaatctg agattatcca atcatacatt
     8881 ttatctttag ctattaattt tcacccgcat gaagttgcat tcctattgat tgactataaa
     8941 ggtgggggta tggcgaactt atttaaagat ttagtccatt tagttggtac gattacaaac
     9001 ttagatggcg atgaagcgat gcgtgcctta acatcaatca aagccgaatt gagaaaacgt
     9061 caacgtttat tcggagagca tgatgttaac catattaatc aataccataa gttatttaaa
     9121 gaaggtattg cgacagaacc aatgccacat ttattcatta tttccgatga atttgccgaa
     9181 ttaaaatcag aacaacctga ttttatgaaa gaacttgtat caacggcacg tattggacgt
     9241 tcgttaggta ttcatttaat acttgcgaca caaaaaccat cgggtgttgt tgatgaccaa
     9301 atttggtcta actctaaatt taaattggca ttaaaagtac aagatagaca agacagtaat
     9361 gaaattttaa aaacaccaga tgcagcagac attacattac caggtcgcgc gtatttacaa
     9421 gttggtaata atgaaattta tgaattgttc caatctgcat ggagtggagc aacatataac
     9481 atcgaaggcg ataaattaga agttgaagat aagacgattt acatgattaa tgactatggt
     9541 caacttcaag caatcaacaa agacttgagt ggacttgaag atgaagaaac gaaagaaaat
     9601 caaactgagt tagaagcggt tatcgatcat atcgaatcta ttacaacacg attagaaatt
     9661 gaagaagtta agcgtccatg gttaccacca ttaccagaaa atgtatatca agaagattta
     9721 gtagaaacag atttcagaaa attatggtca gatgatgcaa aagaagtgga attaacatta
     9781 ggacttaaag acgtaccaga agaacaatat caagggccga tggtattaca attgaaaaaa
     9841 gctggtcaca tcgcgttaat cggaagtcca ggatatggta gaacaacgtt cttacacaac
     9901 attattttcg atgttgcaag acaccatcgt cctgatcaag cacacatgta cttgttcgat
     9961 ttcggaacta atggtttgat gccagtcaca gacataccac atgttgctga ttattttaca
    10021 gtagatcaag aagacaagat tgcgaaggcg atacgtaaga tacatgatat tatttctgag
    10081 cgaaaaagac tattaagtca agagagagta gttaatatag agcaatataa taaagaaact
    10141 ggaaattcaa ttccaaatat tttcttaatt atcgataact acgacacggt gaaagaatca
    10201 ccatttatgg aagaatatga agaaatgatg tctaaggtaa cacgtgaagg tttagcatta
    10261 ggggtataca tcattctttc aggatcaaga tcaagtgcaa taaaatcagc tatatttact
    10321 aacattaaaa caagagttgc actatacctc tttgaaaata atgaattaac aaatattatt
    10381 ggttcttata aaaaaggtgt gaaagatgtt aaaggtagag ctgcaataaa tgatgataac
    10441 ttcacacagt tccaaattgc gcaaccattt gaattagcag aaggacaaac atataacgag
    10501 cgcattaaaa atgaagtagc acaaatgaaa gaattctatg taggagatta tccaaaacat
    10561 atcccaatga tgccagataa ggtattaatg gatgatattc aagaaacata tgatttggaa
    10621 aaaatcattc atgaagaaca caaattacca ttaggattag actttgagga tgtggaacta
    10681 gttggtttcg acttaagtca aacaaatatt ttcacttcag ttaaacctgt tgatatagat
    10741 aatggtttaa ctttattaga gaaacagcta aatataattt caaatgaata tgaaattgca
    10801 actttagaca caaaaggcat attaaaaaat acagggtatg aagattattt gtactgtgga
    10861 gataagaaag aaattattag ttttaaaaat gaattagtga gttttataaa aaatgtagaa
    10921 ccacgaaaaa aatggatagt agttatatcg gattttaaag aatttataaa tattgcatca
    10981 ccaaataatg atgaaataaa aactatattt ttagacggac caaagaataa tgtctttcca
    11041 attatttacg gattgtatca agaaacaatt ggaggattta gttctcaaat aaagttactt
    11101 aaggaaatag tcagtagcgc ttt
//
LOCUS       DAHHOJ010000089        11003 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.089, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000089 DAHHOJ010000000
VERSION     DAHHOJ010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 11003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11003
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.089"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(292..1767)
                     /locus_tag="NBH32_002008"
     CDS             complement(292..1767)
                     /locus_tag="NBH32_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M17 family metallopeptidase"
                     /protein_id="HCD1515590.1"
                     /translation="MNFKLNNTLSNEINTLIIGIPEHLNQLERISFNHIDITESLERL
                     KHQHIIGSKVGKIYTTAFDVQDQTYRLITVGLGNLKARSYQDMLKIWGHLFQYIKSEH
                     IEDTYLLMDSFISKYDQLSDVLMACGIQSERATYEFDHYKSSKKAPFKTNLNLISESL
                     IELDFIHEGISIGQSINLARDFSNMPPNVLTPQTFAEDIVNHFKNTKVKVDVKDYDTL
                     VSEGFGLLQAVGKGSKHKPRLVTITYNGKDKDEAPIALVGKGITYDSGGYSIKTKNGM
                     ATMKFDMCGAANVVGIIEAASRLQLPVNIVGVLACAENMINEASMKPDDVFTALSGET
                     VEVMNTDAEGRLVLADAVYYANQYQPSVIMDFATLTGAAIVALGDDKAAAFESNSKVI
                     LNDILQISSKVDEMVFELPITATERASIKHSDIADLVNHTNGQGKALFAASFVTHFSG
                     QTPHIHFDIAGPATTNKASYNGPKGPTGFMIPTIVQWLKQQ"
     gene            complement(1898..3106)
                     /locus_tag="NBH32_002009"
     CDS             complement(1898..3106)
                     /locus_tag="NBH32_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1515591.1"
                     /translation="MAQDRKKVLVLGAGYAGLQTVTKLQKAISTEEAEITLINKNEYH
                     YEATWLHEASAGTLNYEDVLYPVESVLKKDKVNFVQAEVTKIDRDAKKVETNQGIYDF
                     DILVVALGFVSETFGIEGMKDHAFQIENVITARELSRHIEDKFANYAASKEKDDNDLS
                     ILVGGAGFTGVEFLGELTDRIPELCSKYGVDQNKVKITCVEAAPKMLPMFSEELVNHA
                     VSYLEDRGVEFKIATPIVACNEKGFVVEVDGEKQQLNAGTSVWAAGVRGSKLMEESFE
                     GVKRGRIVTKQDLTINGYDNIFVIGDCSAFIPAGEERPLPTTAQIAMQQGESVAKNIK
                     RILNGESTEEFEYVDRGTVCSLGSHDGVGMVFGKPIAGKKAAFMKKVIDTRAVFKIGG
                     IGLAFKKGKF"
     gene            complement(3560..3919)
                     /locus_tag="NBH32_002010"
     CDS             complement(3560..3919)
                     /locus_tag="NBH32_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HCD1515592.1"
                     /translation="MPTVILTEAAAYEVKDMLKANEMPDGYLKIKVNGGGCTGLTYGM
                     GAEEAPGENDEILEYFGLKVLVDKKDAPVLNGTTIDFKQSLMGGGFQIDNPNAIASCG
                     CGSSFRTAKVAGNPENC"
     gene            complement(3932..4168)
                     /locus_tag="NBH32_002011"
     CDS             complement(3932..4168)
                     /locus_tag="NBH32_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzB family protein"
                     /protein_id="HCD1515593.1"
                     /translation="MNPIVEFCLSNMAKGGDYVFNQLENDPDVDVLEYGCLTHCGICS
                     AGLYALVNGDIVEGDSPEELLQNIYAHIKETWIF"
     gene            4484..5548
                     /locus_tag="NBH32_002012"
     CDS             4484..5548
                     /locus_tag="NBH32_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCD1515594.1"
                     /translation="MKNLVLLGGGYGNMRIMSRILTTSLPQDYTVTLVDRMPFHGLKP
                     EFYALAAGTKSDKDVRMKFPNHPQVNTVYGEINDIDLDAQIVSVGNSKIDYDELIIGL
                     GCEDKYHNVPGAEEYTHSIQTLSKARDTFHSISELPEGAKVGIVGAGLSGIELASELR
                     ESRSDLEIYLYDRGPRILRNFPEKLSKYVAKWFAKNNVTVVPNSNINKVEPGKIYNCD
                     EPKDIDLVVWTAGIQPVEVVRNLPIDINSNGRVIVNQYHQVPTYRNVYVVGDCADLPH
                     APSAQLAEVQGDQIADVLKKQWLNEPLPDKMPELKVQGIVGSLGDKQGFAYIMDRTVT
                     GRLASILKSGVLWLYKYHNG"
     gene            complement(5607..5930)
                     /locus_tag="NBH32_002013"
     CDS             complement(5607..5930)
                     /locus_tag="NBH32_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YuzD family protein"
                     /protein_id="HCD1515595.1"
                     /translation="MEHVSVVVYGADVICASCVNAPTSKDIYDWLQPLLKRKYPNISF
                     KYTYIDITKDNDNLTDHDLQFIERIEQDELFYPLITMNDEYVADGYIQTKQITRFIDQ
                     KLVNE"
     gene            6031..6273
                     /locus_tag="NBH32_002014"
     CDS             6031..6273
                     /locus_tag="NBH32_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HCD1515596.1"
                     /translation="MPTEDTTMFDQVAEVIERLRPFLLRDGGDCSLIDVEDGIVKLQL
                     HGACGTCPSSTITLKAGIERVLHEEVPGVIEVEQVF"
     gene            complement(6531..7706)
                     /gene="dltD"
                     /locus_tag="NBH32_002015"
     CDS             complement(6531..7706)
                     /gene="dltD"
                     /locus_tag="NBH32_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HCD1515597.1"
                     /translation="MKLKPFLPILISGAVFIVFLLLPASWFTGLVNEKTVEDNRTTLT
                     DQVLKGTLIQDKLYESNKYYPIYGSSELGKDDPFNPAIALNKHNANKKAFLLGAGGST
                     DLINAVELASQYDKLKGKKLTFIISPQWFTNHGLTNQNFDARMSQTQINQMFQQKNMS
                     TELKRRYAQRLLQFPHVHNKEYLKSYAKNPKETKDSYISGFKENQLIKIEAIKSLFAM
                     DKSPLEHVKPATKPDASWDEMKQKAVEIGKADTTSNKFGIRDQYWKLIQESKRKVRRD
                     YEFNVNSPEFQDLELLVKTMRAAGADVQYVSIPSNGVWYDHIGIDKERRQAVYKKIHS
                     TVVDNGGKIYDMTDKDYEKYVISDAVHIGWKGWVYMDEQIAKHMKGEPQPEVDKPKN"
     gene            complement(7703..7939)
                     /gene="dltC"
                     /locus_tag="NBH32_002016"
     CDS             complement(7703..7939)
                     /gene="dltC"
                     /locus_tag="NBH32_002016"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     2"
                     /protein_id="HCD1515598.1"
                     /translation="MEFREQVLNLLAEVAENDIVKENPDVEIFEEGIIDSFQTVGLLL
                     EIQNKLDIEVSIMDFDRDEWATPNKIVEALEELR"
     gene            complement(7957..9171)
                     /gene="dltB"
                     /locus_tag="NBH32_002017"
     CDS             complement(7957..9171)
                     /gene="dltB"
                     /locus_tag="NBH32_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PG:teichoic acid D-alanyltransferase DltB"
                     /protein_id="HCD1515599.1"
                     /translation="MIPYGDFTFFLIALIALLPVIILGFLGKRSYIYNGVVTAFMIVL
                     IFSSDKHNLFDQKYLSVQLISFIIYVVWQVLLIMFYYHSKPKNNSFSKFVTVMVLSIL
                     PLALVKVLQSTWLGGHQIHFHESKLIEFVGFLGISYVTFKSVQLIMEIRDGSIKEIKV
                     WKLIQFISFFPTISSGPIDRYKRFVKDDKKVPTGNEYRELVLKAIHMIMLGFLYKYIV
                     AYFINTYAIMPLQLDLHGFVNLWLYMYAYSLYLFFDFAGYSLFAIAFSYLFGIKTPPN
                     FDKPFKAKNIKDFWNRWHMTLSFWFRDCIYMRSLFYMSRKKLLKSQFAMSNVAFLINF
                     FIMGIWHGIEVYYIVYGLYHAALFIGYGYYERWRKKHPPRWQNGFTTALSIVITFHFV
                     TFGFLIFSGKLI"
     gene            complement(9168..10625)
                     /gene="dltA"
                     /locus_tag="NBH32_002018"
     CDS             complement(9168..10625)
                     /gene="dltA"
                     /locus_tag="NBH32_002018"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HCD1515600.1"
                     /translation="MTDIINKLQAFADANPQSIAVRHTTDELTYQQLMDESSKLAHRL
                     QGSKKPMILFGHMSPYMIVGMIGAIKAGCGYVPVDTSIPEDRIKMIINKVQPEFVFNT
                     TDESFESLAGEVFTIEDIKTSQDPVIFDSQIKDNDTVYTIFTSGSTGEPKGVQIEYAS
                     LVQFTEWMLELNKSGNEQQWLNQAPFSFDLSVMAIYPCLASGGTLNLVDKNMINKPKL
                     LNEMLTATPINIWVSTPSFMEMCLLLPTLNEEQYSSLNEFFFCGEILPHRAAKALVSR
                     FPSATIYNTYGPTEATVAVTSIQITQEILDQYPTLPVGVERPGAKLSTTDEGELVIEG
                     QSVSLGYLKNDQKTAEVFNFDDGIRTYHTGDKAKFENGQWFIQGRIDFQIKLNGYRME
                     LEEIETQLRQSEFVKEAIVVPVYKNDKVIHLIGAIVPTTEVTDNSEMTKNIKNDLKSR
                     LPEYMIPRKFEWMEQLPLTSNGKIDRKKIAEVING"
     gene            complement(10641..10793)
                     /locus_tag="NBH32_002019"
     CDS             complement(10641..10793)
                     /locus_tag="NBH32_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HCD1515601.1"
                     /translation="MKSKSKQPPNKYVEAFKPYLLTLLYLAIFITLYLIYGSGDTHNN
                     FIYNEF"
     gene            complement(10841..10951)
                     /locus_tag="NBH32_002020"
     CDS             complement(10841..10951)
                     /locus_tag="NBH32_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515602.1"
                     /translation="MMTTNYYVESIKLKLNFIMNIDIMNCKKQILKRILY"
BASE COUNT         3520 a         1942 c         1613 g         3928 t
ORIGIN      
        1 tctttcttaa aataatttta tctttcatta aattaaacaa ttttacaagc ttggaacacc
       61 aatcaaaata ctaagttcta aaatgtaata ttagtagtcg ttgactgaat gaacatatgc
      121 ttataatatt tttttgcaat gctagtcaag ttgatttatg ctcacaagga tatgcgattt
      181 atattttctt acaacaatga aaatgcctga tacaatgcga tcctttatac tttcaagagt
      241 taaattagaa atgtatatca agacacaagt tgtatcaggc aaataattaa attattgttg
      301 ttttaaccat tgtactatcg tcggaatcat aaatcctgtt ggcccttttg gaccattata
      361 tgaagcttta ttagtcgttg ctggacctgc aatatcgaaa tgaatgtgag gtgtttgacc
      421 actaaaatgt gttacaaaac ttgccgcaaa tagcgcttta ccttgtccat tcgtatggtt
      481 aactaaatca gcgatatcac tgtgtttaat acttgcacgt tcggttgcag taatcggtaa
      541 ttcaaatacc atttcatcga ctttagaact tatttgtaat atatcgttta atatcacttt
      601 actattcgat tcaaatgcag cagctttatc atcgcctagt gcaacaattg ctgcacccgt
      661 taatgtagca aagtccataa tcacactagg ctgatattga tttgcataat acacagcatc
      721 tgcaaggact aatctacctt cagcgtctgt attcattact tctacagttt caccacttaa
      781 tgctgtaaat acatcatctg gcttcattga tgcttcattt atcatatttt cagcacaagc
      841 aagcactccg acaatattta caggcagttg taaacgacta gccgcttcaa tgataccaac
      901 gacattcgca gcgccacaca tgtcaaactt cattgtagcc atgccattct tcgttttaat
      961 actataacca ccagaatcat acgttatacc tttaccaact aaggcaattg gtgcttcatc
     1021 tttgtctttg ccattatatg tgatggttac taatctcggt ttatgcttac tacctttacc
     1081 tactgcttgt aaaagtccga atccttcaga aactaaagtg tcataatctt taacatctac
     1141 tttgaccttt gtatttttaa aatgattaac aatatcttct gcaaatgttt gtggtgttaa
     1201 tacattcggt ggcatattac taaagtctct tgccaaatta atggattggc caatactgat
     1261 accctcatga atgaaatcta attcaattaa tgattcacta ataaggttta aattcgtctt
     1321 aaacggtgcc ttcttacttg atttataatg atcgaattca tatgttgcac gctcactttg
     1381 aataccgcat gccattaata catctgataa ctgatcatat tttgaaataa atgaatccat
     1441 aagtaaataa gtatcttcaa tgtgttctga ctttatgtat tggaaaagat gtccccatat
     1501 tttcaacata tcttgataac tacgtgcttt taagtttcct aaaccaactg taattaaacg
     1561 atatgtttga tcttgtacat caaatgcagt tgtataaatc ttcccaactt tactaccaat
     1621 aatatgttga tgttttagtc tttcaagtga ttctgtaata tcgatatgat taaaactaat
     1681 gcgctctaac tgatttaaat gttctggtat accaataatc aatgtattta tttcgttgct
     1741 tagtgtgtta tttaatttaa aattcataat gtacctcctt agatttgata tgttactcac
     1801 ttcaaattgt acaacaataa agcccttcag tgacactgaa ggactttata aatgaataat
     1861 ttaattttat gtgtttagct gaaactttat ttaaagccta gaatttacct tttttgaatg
     1921 ctaaaccgat accaccgatt ttgaataccg cacgtgtatc aatcactttt ttcatgaatg
     1981 ctgctttttt accagcgata ggtttaccaa ataccatacc tacaccgtca tgtgaaccta
     2041 aagaacaaac agttccacga tcaacgtatt cgaattcttc agttgattca ccgtttaaga
     2101 tgcgtttaat gtttttagca acactttcac cttgttgcat tgcaatttgt gctgtagttg
     2161 gtaatggacg ttcttctcca gctgggataa acgctgaaca gtcaccaata acaaaaatgt
     2221 tgtcgtaacc attgattgtt aaatcttgct ttgtaacgat acgtccacgt ttaacgcctt
     2281 caaatgattc ttccattaat ttactaccac gtacaccagc tgcccatact gaagtacctg
     2341 catttaattg ttgtttttca ccatctactt caactacaaa acctttttcg ttacaagcaa
     2401 cgattggtgt agcaatttta aattcaacac cgcggtcttc taagtagcta actgcgtggt
     2461 taactaattc ttctgagaac attggtaaca ttttaggtgc tgcttcaaca caagtgattt
     2521 taactttatt ttgatccaca ccatatttgc tacataattc aggaattctg tctgttaatt
     2581 cacctaagaa ttcaacacca gtgaatccag caccaccaac taagatagat aaatcgttat
     2641 catctttttc ttttgatgct gcatagttag caaatttgtc ttcgatatga cgtgataatt
     2701 cacgtgctgt gataacattt tcaatttgga aagcatgatc tttcatacct tcgatgccga
     2761 atgtttcact aacgaaacct aatgctacta ctaaaatatc aaagtcataa ataccttgat
     2821 ttgtttctac ctttttagca tcacggtcaa tttttgttac ttctgcttga acaaagttca
     2881 ctttgtcttt cttcaagaca ctttccacag gatataatac atcttcatag tttagtgtac
     2941 ctgctgatgc ttcatgtaac catgttgctt catagtgata ttcattttta ttaataagcg
     3001 taatttctgc ttcttctgtt gatatcgctt tttgcaattt agttacagtt tgtaaacctg
     3061 cataaccagc accaagtaca agtacttttt tacgatcttg agccatttaa tttcacctaa
     3121 gctttcatat ttttttaacc aaatgctgat aattactagt aacaactggt cattactact
     3181 tttaccacaa tgtatactag tttatcatca ttttgttatt taagtctttt aaattatgta
     3241 aaaacctgtg atgttcacaa aaaagacatt tttaatccaa tactaattct atagctttta
     3301 tgttatattt tcaagcaaga ttcagtcaga ttgtgaaaaa attaattaat tttttcgtag
     3361 cctgatttcg attaaaaatg tatccgttat cacgatcatt taaaatacta tttatagtca
     3421 gattcttaat tttcaataag ctttttaata aaacaaaata tctttacgca aaaataggac
     3481 ataatcaaat atgtccctaa tcataattga tatcaatttg tatcccaaaa attgggttaa
     3541 ccattaaaaa gttattttat tagcagtttt caggattacc tgcaactttt gcagttctaa
     3601 atgaactacc acagccacat gaagcaattg cattaggatt gtcgatttgg aaaccgccac
     3661 ccattaatga ttgcttaaaa tcaatagtcg taccatttaa tacgggtgca tcttttttgt
     3721 ctactaaaac ttttaatcca aagtattcta agatttcatc attttcacca ggcgcttctt
     3781 ctgcacccat accgtatgtt aaaccagtgc acccgccacc attcacttta atttttaaat
     3841 agccatctgg catttcattt gctttaagca tatcttttac ttcgtaagca gctgcttctg
     3901 ttaatataac tgttggcatg ataactcctc cttaaaaaat ccaagtttct tttatatgtg
     3961 catatatatt ttgtaataat tcttccggcg aatcaccttc aacaatatca ccatttacta
     4021 aagcatacaa cccggctgaa catataccac aatgtgtcag gcaaccatac tctaacacat
     4081 cgacatctgg gtcattttcc agttgattaa aaacataatc tccacctttt gccatgttag
     4141 agagacaaaa ttctacgatc ggattcatac ttcaccttct tatttcattt gttacaatat
     4201 tatagcattt taaaactggt attttaacat gatgtgctca attacaacaa ctgatgtttc
     4261 ttatcccagt tatgtaatag tgccttagtt agtacaaata tttatttgta gtcattttca
     4321 aaaaaatata taataagcat ataaatacat atttactttt ttaggggtta ttgatgaatc
     4381 aaagacactt gcattttata actgttcatt aacaaatata tctatgcact ttgatctttt
     4441 caaaattaaa gtaaatatta acatataaaa ggggtacttc attatgaaaa acttagtttt
     4501 gttaggcggc ggatatggta atatgcgtat catgtcacgc attttaacta cttctttacc
     4561 acaagattat acagtcacat tagttgatcg tatgccattt catggattga aaccagaatt
     4621 ttatgcttta gctgcgggca cgaaatcaga taaagatgtt cgcatgaaat tccctaatca
     4681 tccacaagtg aatacagttt atggtgaaat taacgacata gatttagatg ctcaaattgt
     4741 ctcagtcggt aactctaaaa ttgattatga tgagctaatc attggtttag gatgtgaaga
     4801 taaatatcat aacgttccag gagccgaaga atatacacat agtattcaaa cactctcaaa
     4861 ggctcgggat actttccata gtattagtga actaccagaa ggtgctaaag ttggtatcgt
     4921 tggtgctgga ttaagcggca tagaacttgc cagcgaatta agagaaagta gatcagactt
     4981 ggaaatatat ctttatgacc gtgggccgcg aattttaaga aattttccag aaaaattaag
     5041 taagtatgtt gcgaaatggt tcgccaaaaa taatgttacc gttgttccaa attcaaatat
     5101 taataaagtt gaacctggta aaatatataa ctgtgatgaa cctaaagata ttgatttagt
     5161 tgtatggaca gcaggaattc aacctgttga agttgttcgt aacttgccga ttgatataaa
     5221 tagtaatgga cgcgtgatag ttaaccagta tcatcaagta ccaacatatc gtaacgtcta
     5281 tgtagttggt gattgtgctg atttaccaca tgcgccaagt gctcagttag ccgaagttca
     5341 aggtgatcaa attgccgatg tgcttaaaaa gcaatggcta aatgaaccat tacctgacaa
     5401 aatgccggaa ctaaaggtac aaggtatcgt tgggtcatta ggagataaac aaggttttgc
     5461 atatatcatg gatcgcactg taacaggacg actcgcttct attttaaaat ccggcgtact
     5521 ttggttatat aaatatcata atggttaaaa ttaaaaagca atgtcagttc acactattac
     5581 tgacattgct ttttgttatg tgtatattat tcattcacaa gcttttggtc tataaatcga
     5641 gtgatttgct ttgtttgtat ataaccatct gctacatatt catcgttcat tgtaattaat
     5701 ggataaaata gttcatcttg ttctattctt tcaataaatt gtaaatcatg atctgttaag
     5761 ttgtcattat cttttgtaat atctatatac gtatatttaa atgatatatt tgggtatttt
     5821 ctttttaata gcggctgtag ccagtcataa atatctttcg atgttggcgc attaacgcaa
     5881 cttgcacata taacatctgc cccatatact accacactca cgtgctccat atatttatcc
     5941 cccattggtt tgatagattt ttattacact atctattata atatactaat aaagattatc
     6001 aaattcatct ctcgaaagga gacttgcctg atgcctactg aagatacaac gatgtttgat
     6061 caagtagcag aagttattga acgtcttcgc ccatttttat tacgtgatgg tggcgactgc
     6121 tcattgattg acgtggaaga cggtattgtt aaattacaat tacatggtgc atgtggtaca
     6181 tgcccaagtt ctacaatcac tcttaaagct ggtattgagc gtgtattaca cgaagaagtg
     6241 cctggtgtaa tagaagtaga acaagtattc taatataaag atattaaatt aacagaacat
     6301 attatgaata acaacaaaga caggaatact gaattattta taatcagtta ttacctgtct
     6361 tttttcgctg tcaaattgga ctgatgggaa agttatttta tactatttat ctgattaatt
     6421 attttattat tgccacatat ccaagcgcat attactattc tatgaaattt aaaatataaa
     6481 aatagcacat acgctcaatc aaaatcgttg agttatgtgc tatttgtatt ttaattttta
     6541 ggtttatcta cttcaggttg tggttcacct ttcatatgtt tcgcaatttg ctcatccata
     6601 taaacccaac ctttccaacc gatgtgtacg gcatcactga taacatattt ttcataatct
     6661 ttatcagtca tatcgtaaat tttaccacca ttatctacaa cagtagaatg gattttttta
     6721 tacactgctt gacgacgttc tttatcgata cctatgtggt cataccatac accgtttgat
     6781 ggaatactta catattgaac atctgcacca gcagcacgca ttgtttttac aagtaattct
     6841 aaatcttgga attctggaga attaacattg aattcgtagt cacgtctaac tttacgctta
     6901 ctttcttgaa ttaatttcca gtattgatct ctaataccaa atttattcga tgtagtatca
     6961 gctttaccaa tttcaactgc tttttgtttc atctcatccc aagaagcgtc tggttttgta
     7021 gcaggtttaa catgttctaa tggagattta tccattgcaa acaatgattt aatcgcttct
     7081 attttaatca attgattctc tttaaaacca gaaatataac tatctttagt ttctttaggg
     7141 tttttagcat aagatttcaa gtattcttta ttgtgtacat gtggaaactg taataaacgt
     7201 tgtgcataac gacgttttaa ttcagtagac atgtttttct gctggaacat ttgattaatt
     7261 tgagtttgag acatacgagc atcaaagttt tgattcgtta aaccatggtt tgtaaaccat
     7321 tgtggtgaaa taataaatgt taatttctta ccttttaatt tatcatactg tgatgcaagt
     7381 tcaactgcgt taattaagtc tgtagaacca ccagcaccta ataagaatgc ttttttgttg
     7441 gcgttatgct tatttaatgc aattgcagga ttaaatgggt catctttacc taattcacta
     7501 gagccgtata taggataata cttgtttgat tcgtataact tatcttgaat gagtgtgcct
     7561 tttagtactt gatctgtcaa tgtagttcta ttatcttcta cagtcttttc atttactaat
     7621 cctgtaaacc aactagcagg taataataga aatacaatga ataccgctcc actaattaaa
     7681 atgggtaaaa aaggttttaa tttcatcgta actcttctaa tgcttcaacg attttatttg
     7741 gtgttgccca ctcatctcta tcaaagtcca taatagatac ttcgatatca agtttatttt
     7801 gaatctctaa taataatcca actgtttgga aagaatcaat aataccttct tcaaaaattt
     7861 ctacgtctgg attttctttt acaatatcgt tttctgctac ttctgctaat aaatttaata
     7921 cttgttctct aaattccata attaaattct cctttattat ataagtttac ctgagaagat
     7981 taaaaagcca aatgttacaa agtggaatgt gatcacaatg ctaagtgctg ttgtgaaacc
     8041 attttgccaa cgtggcggat gtttcttgcg ccaacgttca taatagccat aacctataaa
     8101 caatgctgca tggtataaac cataaacaat gtaatacact tcgataccat gccaaattcc
     8161 cattatgaag aagttgatta agaatgccac gttagacatt gcaaattgac tcttcaataa
     8221 ttttttacga gacatgtaga ataaagatct catgtaaata caatctctga accagaatga
     8281 taatgtcata tgccatctat tccagaaatc tttaatattt ttcgctttga aaggtttatc
     8341 gaagtttggt ggtgttttaa taccgaataa ataactaaat gctatcgcaa ataaactata
     8401 acctgcaaag tcaaagaata aatataagct gtatgcgtac atatataacc acaaattgac
     8461 aaaaccatgt aagtctaatt gtaacggcat gattgcatat gtgttaataa agtaagcaac
     8521 aatatattta tacaagaaac caagcataat catgtgaatt gcttttaata ctaattcacg
     8581 atattcattg cctgttggta cttttttatc gtctttaacg aaacgtttgt aacggtcgat
     8641 tggtccagat gaaatcgttg ggaagaatga aataaattga attaatttcc atactttaat
     8701 ttctttgata gaaccatcac gaatttccat aattaactgc acacttttga atgtaacata
     8761 agaaattcct aagaaaccaa caaattcaat taatttgctt tcatggaagt gaatctgatg
     8821 cccacctaac catgtacttt gtaacacttt cacaagtgct aatggcaata ttgataaaac
     8881 cattacagtt acaaattttg aaaatgaatt attttttggt tttgaatgat aataaaacat
     8941 tatcaataaa acttgccata cgacgtaaat aataaaacta attaattgaa cacttaaata
     9001 cttttggtca aacagattat gtttatcaga agaaaagatt aacacaatca taaatgctgt
     9061 aactacgcca ttataaatgt aacttcgctt acctaaaaat ccaagtataa tgactggtaa
     9121 taatgcaatt aaagcaatta agaagaatgt aaaatcacca tatggaatca tccgttaatt
     9181 acctctgcaa ttttctttct gtcaatttta ccatttgaag tcaatggtaa ttgttccatc
     9241 cattcaaact ttctaggaat catatactct ggtaagcgtg atttcaagtc atttttaata
     9301 tttttagtca tttctgaatt gtccgtaact tcagtcgttg gcacaattgc accaattaaa
     9361 tgaataactt tatcattttt atatacaggt acaacaatcg cttcttttac gaactcagac
     9421 tggcgtaatt gtgtttcaat ttcttctaat tccattctgt agccattcaa tttgatttgg
     9481 aaatcaatac gaccttgaat gaaccattga ccattttcaa acttcgcttt atcaccagtg
     9541 tgataagtac gaataccgtc atcgaaatta aatacttcag ctgttttttg gtcatttttt
     9601 aagtatccta aacttacact ttgaccttcg ataacaagtt caccttcatc tgtagtagat
     9661 aattttgcgc ctggtctttc aacaccaaca ggtaatgtcg gatattgatc taagatttct
     9721 tgtgtaattt gaatacttgt aactgctacc gtagcttcag ttggaccata tgtgttgtaa
     9781 atcgtcgcac ttgggaaacg gcttactaac gcttttgctg ctctgtgagg tagaatttca
     9841 ccacagaaga agaattcgtt aagactacta tactgttctt cattaagcgt tggtaataat
     9901 aaacacattt ccataaatga tggtgttgat acccaaatgt taatcggtgt tgctgttagc
     9961 atttcattta ataatttagg tttattaatc atgtttttat ctacaagatt taatgtaccg
    10021 cctgatgcta aacatggata aatagccatt acagataagt caaatgaaaa tggcgcttgg
    10081 ttaagccatt gttgttcatt tcctgattta ttaagttcta acatccactc agtaaattga
    10141 actaaacttg catattcaat ttgaacacct ttaggttccc cagtagaacc agatgtaaag
    10201 attgtgtata ctgtgtcgtt atctttaatc tgactatcaa aaattactgg gtcttgagat
    10261 gttttaatat cttctattgt aaatacttcg cctgctaaac tttcaaatga ttcatcagtc
    10321 gtattaaata caaactctgg ttgaaccttg ttaataatca ttttaatacg gtcttcagga
    10381 attgaagtgt ctacaggtac atatccacat cctgctttaa tggcacctat catcccaaca
    10441 atcatatatg gtgacatgtg accgaataaa atcatcggtt tcttactacc ttgtaatcga
    10501 tgtgctaatt tactagactc atccattaac tgttgataag ttaattcatc agttgtgtgt
    10561 ctaacagcaa tgctttgtgg atttgcatcc gcaaacgctt gcagcttatt aataatatct
    10621 gtcatattaa gtctccctca ttagaactca ttataaatga agttattgtg tgtgtcgcca
    10681 ctgccataaa ttaaatataa agtaataaat attgccaaat acaatagtgt taataaatat
    10741 ggtttgaatg cttcaacata tttattaggt ggctgtttac ttttagattt catattgcac
    10801 ctcttaaagt tcttagtaaa aacgccttta taaagaccgt tcaatataaa atacgtttta
    10861 aaatttgttt tttacaattc attatatcga tattcataat gaaattcaat tttaatttta
    10921 tagattcaac atagtaattg gttgtcatca ctcaattatt gtaaatgata cactatgttg
    10981 agaacatcat tcattttaaa ggt
//
LOCUS       DAHHOJ010000090        10999 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.090, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000090 DAHHOJ010000000
VERSION     DAHHOJ010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10999)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10999)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10999
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.090"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            7..1464
                     /locus_tag="NBH32_002021"
     CDS             7..1464
                     /locus_tag="NBH32_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500488.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCD1515603.1"
                     /translation="MKCLIRFILVLGLLISSAMVYINPTAHAEQDQTWEKIKERGELR
                     VGLSADYAPMEFEHTVNGKTEYAGVDIDLAKKIAKDNNLKLKIVNMSFDSLLGALKTG
                     KIDIIISGMTSTPERKKQVDFSDSYMMTKNIMLVKKDKVNEYKDIKDFNNKKVGAQKG
                     TEQEKIAQTEIENASITSLSRLPDVILALKSGKVEGAVVEKPVAEAYLKQNPKLGISN
                     VKFNEEEKDTVIAVPKDSPKLLSQINKTIKEVKDKGLIDKYMTNAANAMNDDSGFISK
                     YGSFFLKGIKITILISLIGVALGSILGAFVALMKLSKIKIISWIASIYIEILRGTPML
                     VQVFIVFFGITAALGLDISALVCGTIALVINSSAYIAEIIRAGITAVDKGQMEAARSL
                     GLNYRQTMKSVIMPQAIKNILPALGNEFVTLIKESSIVSTIGVGEIMFNAQVVQGISF
                     DPFTPLLVAAALYFVLTFVLTRIMNMIEGRLNASD"
     gene            1457..2179
                     /locus_tag="NBH32_002022"
     CDS             1457..2179
                     /locus_tag="NBH32_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCD1515604.1"
                     /translation="MIKINNLNKVFGDNEVLKDINLEINQGEVVAIIGPSGSGKSTLL
                     RCMNLLEVPTKGQVIFEGNDLTEKGTQVDKLRQKMGMVFQNFNLFPHKKVVDNIILAP
                     KLLKKDNNDELHKEALSLLDKVGLKEKADVYPNQLSGGQKQRVAIARALAMHPDVILF
                     DEPTSALDPEVVGDVLKVMKDLAKEGMTMVVVTHEMGFAKDVSDKVIFMADGVVVESG
                     TPVEIFEQPQHERTQNFLARVL"
     gene            2329..3456
                     /gene="queG"
                     /locus_tag="NBH32_002023"
     CDS             2329..3456
                     /gene="queG"
                     /locus_tag="NBH32_002023"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000379983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCD1515605.1"
                     /translation="MDTKQLKQDIIDYAYTIGIDSIGFTTADPFDELKQKLEAYHANG
                     YASGFEESDIALRTEPKLSLPTARSIIAIAVGYPNKLKGAPKSVRGDRRGLFARASWG
                     QDYHTIMRKRLDMLAAFIESKVPDVEIKSMVDTGVLSDRAVAERAGLGFVGRNGFVIN
                     PKLGTWTYLGEMLVSIPFEPDDPLLDSCGDCTICVDRCPTSALVGNGQLNSQKCISFL
                     TQTKGYMPDQYRYKIGNRLYGCDTCQQVCPKNRGINTEQDDIILEPEILKPRLVPLLR
                     MSNKEFKQTYGHLAGAWRGKKPIQRNAILALAHFNEVDAIPELKKVATTDERPMIRAT
                     AYWAIGQILGEEARDFINANYDQEDAEVQNEMIKGLDTRRE"
     gene            3461..3931
                     /gene="trmL"
                     /locus_tag="NBH32_002024"
     CDS             3461..3931
                     /gene="trmL"
                     /locus_tag="NBH32_002024"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCD1515606.1"
                     /translation="MTNHIVLYQPEIPANTGNIARTCAGTNTHLHLIKPLGFRTDDKM
                     LKRAGLDYWEFVNITYHDSIEAFFEATNGEYYLLTKFGKKTYSDFDFSNHDKDFYFIF
                     GKETTGLPDWVKEKYQDTALRIPMSEHIRSLNLSNTAALLIYEALRQQDFPGLN"
     gene            4090..4689
                     /locus_tag="NBH32_002025"
     CDS             4090..4689
                     /locus_tag="NBH32_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate isomerase"
                     /protein_id="HCD1515607.1"
                     /translation="MAMNFKVFDNSQLVAEYAADIIRKQFNNNPTTIAGFHLDTDQAP
                     VLDELKKNVEKHAVDFSQINILDYDDKKSYFEALGVPAGQVYPIAYEKDAIELIADKI
                     KTKENKGKLTLQVVSIDEQGKLNVSIRQGLMEAREIFLVVTGANKRDVVEKLYQENGK
                     TSFEPADLKAHRMVNVILDKEAAAGLPEDVKAYFTSRFA"
     gene            4714..4866
                     /locus_tag="NBH32_002026"
     CDS             4714..4866
                     /locus_tag="NBH32_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515608.1"
                     /translation="MSKDKDPKLNYHEEENSMVTDFEDLKELSKEMEQISDQNDQEKN
                     SEEDSQ"
     gene            5701..6096
                     /locus_tag="NBH32_002027"
     CDS             5701..6096
                     /locus_tag="NBH32_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515609.1"
                     /translation="MLGKYWIQLLIATVIISLISIKAFPLAIGALYLPVIFKVIKLQL
                     NLSKGLIDDVNAQTFIKSNQSGIVISVICCLLITGILYYTLDGFYASLTGVLGTLVAL
                     NPYTTIVSAVLYILTAIATVEATKTKYRN"
     gene            6292..7677
                     /gene="fumC"
                     /locus_tag="NBH32_002028"
     CDS             6292..7677
                     /gene="fumC"
                     /locus_tag="NBH32_002028"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000116229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCD1515610.1"
                     /translation="MSVRIEHDTFGEIEVPADKYWGAQTERSKRNFPVGKERMPIEVV
                     YGFAQLKRAAALANFDLGKLSEAKKDAIVYACDQILSGELDEHFPLVVWQTGSGTQSN
                     MNVNEVVSYVANMYLKDHQSDESIHPNDDVNKSQSSNDTFPTAMHVALYQEVETKLEP
                     ALKLLRNTLKEKEDKFDSIIKIGRTHLQDATPIKLGQEISGWRYMLDRCEIMLSESKK
                     HILNLAIGGTAVGTGINAHPEFGDKVAQYISENTGYPFVSSENKFHALTAHDEVVQLH
                     GTLKALAGDLMKIANDVRWLASGPRAGLAEISIPENEPGSSIMPGKVNPTQCEMLTMV
                     AVQVMGNDTVVGFASSQGNFELNVYKPVIMHNTLQSIYLLADGMETFNNNCAVGIEPI
                     EENIDNYLNQSLMLVTALNPHIGYEKAAQIAKKAHKEGLTLKESAIQTGYVTEEQFEA
                     WIKPEDMVDPH"
     gene            complement(8139..8960)
                     /locus_tag="NBH32_002029"
     CDS             complement(8139..8960)
                     /locus_tag="NBH32_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HCD1515611.1"
                     /translation="MKFKIPENFNDLSLRDIFQQLKVPKKDLHHLNMSKDITINDKPA
                     RLMDKVHTGDDVFVPTTDEKSNYVPSYRYAQIKYEDDDMAIVMKPKGVKTHPNDLKES
                     NTLMNHVIYTVDSDYVEPIHRLDQETVGLLIVAKNPLMKKILDRMLEDNDITRIYKAN
                     VKALLPLKPQTIDMPIGKDKFHSNKRRVSPTGQRAITHILTSKMIKETVCQLEIKLDT
                     GRTHQIRVHLAEIGHPVIGDPLYGDSTLRQLELESYKIEFVHPLTKEVISVSLDD"
     gene            9123..10235
                     /locus_tag="NBH32_002030"
     CDS             9123..10235
                     /locus_tag="NBH32_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HCD1515612.1"
                     /translation="MEQRTRLALLKEIAEFLNEETEMYSMTQGALKYLIEGSNFTTGW
                     IFFINSVGEHELVSHVALPQSLTADHCHYIKDGSCWCVKAFNQGRLMKASNIVNCSRI
                     NLASKAFPSQNDNITHHATVPLKSGQEQFGILNVASPNTEIYSDEDLELLESVAFQLG
                     SAIKRIYLTDREKEAAKINERNRLARDLHDSVNQMLFSVKLTAHAAYGVSNESIAKQA
                     FKTIEETSQNAVNEMRALIWQLKPVGLEQGLIHALTAYSKLMHIQLNVNVEGLIDLSN
                     EIEENIYRALQECINNVKKHADTNKMDLTLKQMNDILYIDVIDYGQGFEIDNVQIASS
                     HGINNIKQRVKLLRGKVTFHSQPTKGTQIQFTIPIK"
     gene            10257..10880
                     /locus_tag="NBH32_002031"
     CDS             10257..10880
                     /locus_tag="NBH32_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1515613.1"
                     /translation="MNKVILVDDHYIVRQGLRFLLSTIENIEVLQDFADGETFLEYLK
                     EHEHPDIVLLDLVMPGMNGIEITEYIKAHYPDIKVLVLTSYVDDEHVISAINKGADGY
                     EMKDVEPQQLIETIRRVMNGEKMIHPKAQDVFETVSQKPHYTNKLSKREIEVLREMVK
                     GKTNKEIAETLFVSEKTIKTHVSHIFSKLQVSDRTQAAIYAMENKLI"
BASE COUNT         3968 a         1554 c         2030 g         3447 t
ORIGIN      
        1 gtataaatga agtgtttaat taggtttata ttagtattag ggttgttaat tagcagtgct
       61 atggtgtata tcaatcccac agcacatgct gaacaagatc aaacatggga gaagattaaa
      121 gaacgcggtg aacttagagt gggtctttct gcagattatg caccaatgga atttgagcat
      181 acagttaatg gtaagactga gtatgcaggt gtagatattg atttagctaa aaaaattgcg
      241 aaagataata atttaaaatt aaaaatcgtc aatatgtcat ttgatagttt gttaggagct
      301 cttaaaactg gaaaaattga tattattatt tccggaatga cttcaacgcc tgaacgtaag
      361 aagcaagttg atttttcaga ttcatatatg atgactaaaa atatcatgct tgtaaagaaa
      421 gataaagtta atgaatataa agatatcaaa gactttaata ataaaaaagt aggggcacaa
      481 aagggaactg aacaagaaaa aatcgctcaa accgaaattg aaaatgcatc tattacttca
      541 ttaagccgtt taccagacgt tatattagct ctgaaaagtg gaaaggttga aggtgctgta
      601 gttgaaaaac ctgtagcaga agcatattta aaacaaaatc ctaaattagg aatttccaat
      661 gtgaaattta atgaagaaga aaaagataca gtgatagcag tgccaaaaga ttcaccaaaa
      721 ttattgtcac aaattaataa aacgattaaa gaggttaaag ataaaggatt aatcgataaa
      781 tatatgacta atgctgcaaa tgcgatgaat gatgacagtg gttttatttc taagtatggt
      841 agttttttct tgaaaggaat taagattaca atattaattt cacttatcgg tgttgcatta
      901 ggttctattt taggtgcatt cgttgcgtta atgaaattaa gtaaaataaa aattatttca
      961 tggattgctt ctatctatat cgaaatatta agaggaacac caatgttagt tcaagtattt
     1021 atcgtattct ttggtataac tgctgcatta ggactagaca tttcagctct tgtttgtgga
     1081 acaattgcat tagttattaa ttcttcagct tacattgctg aaattattcg tgcaggtata
     1141 actgctgttg ataaaggcca aatggaagcc gcacgtagtc taggtttgaa ttatagacaa
     1201 acgatgaaaa gtgtaattat gccacaagca attaaaaata ttttaccagc tttaggtaat
     1261 gaatttgtca ctttaattaa agaatcatct attgtgtcaa caattggagt tggcgaaatt
     1321 atgtttaatg cacaagtggt tcaaggtatt tcatttgacc cattcacacc attactagtg
     1381 gcagcagcat tatactttgt cttaacattt gtacttacac gcattatgaa catgattgaa
     1441 gggagattga atgccagtga ttaaaataaa caatcttaat aaagtttttg gagataatga
     1501 agttttaaaa gatatcaatc ttgaaatcaa tcaaggggaa gtagtagcaa taataggtcc
     1561 atctggtagt ggtaaaagta cattgttaag atgtatgaat ttattagaag tacccactaa
     1621 aggtcaagtg atttttgaag gcaatgactt aacggaaaaa gggacacaag tagataaact
     1681 acgacaaaaa atgggtatgg tatttcaaaa cttcaaccta tttccacata aaaaagttgt
     1741 cgataatatt attttagctc ctaaattatt aaagaaagat aataacgatg aattgcataa
     1801 ggaagcattg tcgttattag ataaagtggg attaaaagaa aaagcagatg tatatccgaa
     1861 tcaattatca ggtggtcaaa aacaaagggt agcaattgca agagctttag caatgcatcc
     1921 agatgttatt ttattcgatg aaccaacttc agcattagat cctgaggtgg ttggtgatgt
     1981 attaaaagta atgaaagacc tagccaaaga aggtatgacc atggtggttg tgacacatga
     2041 aatgggattt gccaaagatg taagtgacaa agttatattt atggcagatg gcgttgtcgt
     2101 agagtcaggc acaccagtcg aaatatttga acaaccgcaa catgaaagaa cacaaaattt
     2161 cttagcaaga gtattataac aacctaacga agcttgaata tatgatgcgc accacaaagt
     2221 tatatcatat attcaagctt tttattatta aaatcatgga aatgaaacct caatatgaat
     2281 aatggttgtg ttaaaataac atagtattat tttatggggg gcatacgatt ggatacaaag
     2341 cagttaaagc aagatatcat tgactatgca tatacaattg gtatcgacag tattggattt
     2401 actactgccg atccctttga tgaattgaag caaaagctag aagcatatca tgcaaatggt
     2461 tatgcctcag gatttgaaga atctgatatt gctttacgaa cggagcctaa attatcttta
     2521 ccaacagcaa ggtcaatcat agcaattgca gttggttatc ctaacaaact gaaaggtgca
     2581 cctaagagtg ttagaggtga tcgcagaggc ttatttgcta gagcatcgtg gggtcaagat
     2641 tatcatacaa ttatgcgtaa acgattagac atgttagctg catttattga atctaaagtt
     2701 ccagatgttg aaatcaaatc tatggtagat acgggtgtat tatcagatag ggcagtagca
     2761 gaacgtgcag gtttaggatt tgttggtcgt aatggctttg tcatcaatcc taaactagga
     2821 acatggacat acctcggtga aatgttagtc agtatacctt ttgaacctga tgatccatta
     2881 ttagatagct gtggtgattg tacaatttgt gttgatcgtt gtccaacaag tgcattagtt
     2941 ggaaatggcc aattaaatag tcagaaatgc attagttttt taacacaaac aaaaggctat
     3001 atgcctgacc aatatcgtta taaaattggg aatagattat atggttgcga tacgtgtcaa
     3061 caagtttgcc cgaaaaatag aggtattaat accgaacaag atgacatcat tttggaacca
     3121 gaaattttaa agccaagatt ggtaccttta ctacgtatgt ctaataaaga attcaaacaa
     3181 acatatggtc accttgcagg tgcttggcgt ggtaaaaagc cgatacaacg aaatgctatt
     3241 ttagcattag cgcattttaa tgaagtggat gcaattccag aattgaaaaa agttgcaaca
     3301 acagatgaaa gaccgatgat tcgagcaact gcttattggg ccattggtca aattcttggg
     3361 gaagaagcaa gagattttat caatgctaat tatgatcaag aggatgcaga agttcagaat
     3421 gaaatgataa aaggattaga cacaaggaga gaatagtaat atgacaaatc atatcgtttt
     3481 atatcaacca gaaatcccag ctaatactgg taacattgca cgtacgtgtg caggtacaaa
     3541 cacacattta catttaatta aacctcttgg atttagaact gatgataaaa tgttaaaaag
     3601 agccggttta gattattggg aatttgtgaa tattacgtat catgatagta ttgaagcatt
     3661 ttttgaagcc acaaatggcg agtattactt attaacaaaa tttggtaaaa agacttatag
     3721 tgattttgat ttttcaaatc atgacaaaga tttttacttc atttttggaa aagagacgac
     3781 aggattacca gactgggtga aagagaaata tcaagacaca gcattaagaa ttcctatgag
     3841 tgaacatatt cgttcattaa atttatcaaa tactgctgcg ttgttaattt atgaagcgtt
     3901 acgtcaacaa gattttccgg gattgaatta aaatttactc attttcatta taaaaatgat
     3961 gcttaagtgc tatatttaga gtataagcat gttaaagtta aatggtttga tatattgaaa
     4021 gttagggtat aataattatt aatatcgccg tttttatgta aaatgcaaac taagaggagt
     4081 gtagaaatca tggcaatgaa ctttaaagtc tttgacaata gtcaacttgt agcagaatat
     4141 gctgctgata ttattagaaa gcaatttaac aataatccta ctacaattgc aggttttcat
     4201 ttagatacag atcaagcgcc agttctagat gaattaaaga aaaatgttga aaaacatgct
     4261 gttgatttta gccaaataaa tattttagat tatgacgata aaaaatcata tttcgaagcg
     4321 ttaggtgtac cagcaggtca agtttatcca attgcttatg aaaaagatgc aatcgaatta
     4381 atcgctgata agattaaaac taaagaaaat aaagggaaat taacattaca agttgtttct
     4441 atcgatgagc aaggtaagtt aaatgttagt attcgtcaag gactaatgga agcaagagaa
     4501 attttcttag tagtgacagg tgctaataaa cgagatgtag ttgaaaaatt atatcaagaa
     4561 aatggtaaaa caagcttcga accagccgat ttaaaagcac atagaatggt aaatgttatt
     4621 cttgataaag aagcggctgc aggtttacct gaagatgtta aagcttactt tacgtcacgc
     4681 tttgcttaat taaaaatctg tgaggtattg attatgtcta aagacaaaga tccaaaatta
     4741 aattatcatg aagaagaaaa cagtatggta actgattttg aagatttaaa agaattaagt
     4801 aaagaaatgg aacaaatttc cgatcaaaat gatcaagaaa aaaattctga agaagacagt
     4861 cagtaaagta ttataattgt gtaatattca gctatttatc atgtaaacaa tgtatagtat
     4921 agaacatata tcaaatagga ttgattatct tgaatatgaa gttaggtagc attttgttac
     4981 ctagcttttt tataatattc atagaagctg actttccgcc agcttctgtg ttggggcacc
     5041 gccaactcgc acgttattgt aaggtgactt tccgccagct tctgtgtttt tggcccgttt
     5101 ccccaacttg cattgtttgt agaaattgag gattcaattt cactatgttg gggccccgcc
     5161 aacttgcatt gtctgtataa attggaaatc cgatttcgct atgttgtggc cccgccaact
     5221 tgcatgttat tgtaagctga ctttccacca gttcctgtgt tgtggccgtt tccccaactt
     5281 gcatcatctg tagaaataga gaatccaatt tcaatgtgtt tgtggcccgt ttccccaact
     5341 tgcatcatct gtagaaatta gaaatccgat ttcactatgt tgggcctcgc caacttgcat
     5401 gaaatagtaa gctgactttc cgccagcttc tatgtttgtg gcccgcagtc aactactgcc
     5461 aatataactt tgtagagaat agaacattga tttgtgcacc agacatagta atatatcaaa
     5521 agttcaattt taaatattaa aacaccattt aaacggaatt gataattaat tactccagac
     5581 ataattgaaa gaagtcatct atcagtaata tttttaacga aatcattaaa ccgcgtttct
     5641 taatatgcca agattatgat atgctattga agaatgaaat tagaggagat ttatatattt
     5701 atgttaggaa aatactggat tcaattattg atagcaacag ttattataag tttaatatca
     5761 attaaagctt ttccattggc aattggggca ttatatttac ctgttatttt caaagtaata
     5821 aaattacaac tcaatttatc aaaaggatta atcgatgatg ttaatgcgca aacattcatt
     5881 aaaagtaatc aatcaggtat tgtaataagt gttatttgtt gtttattaat aacaggaata
     5941 ttgtattata cacttgatgg gttttatgcg agtttaacag gagttttagg tacgcttgtt
     6001 gctttaaatc catatacgac aattgttagt gctgtattat atatacttac agctattgct
     6061 acggttgaag ctactaaaac aaagtatcgt aattaaactt agttcaacaa taactaaata
     6121 atgatagata acacactaat cgtatgaccg tatcactgta ataaagggtg agtacgtgcg
     6181 attagtgtat ttttatttca aattatgaaa acgttttaat cataaattgc tcaattaaat
     6241 tatttaatga tatactacag ttaaataaac agtgataagg ggagaaattg aatgtcagta
     6301 agaattgaac atgatacttt tggagaaata gaagtacctg cagataaata ttggggtgct
     6361 caaacagaaa gaagtaaacg caatttccca gttggtaaag agcgtatgcc aatcgaagta
     6421 gtttatggtt ttgcacaact aaagcgtgca gcagcattag ctaattttga tttaggaaaa
     6481 ttaagcgagg caaagaaaga tgccattgta tacgcatgtg atcaaatttt atcaggtgaa
     6541 ttagatgaac acttcccact agttgtatgg caaacaggaa gcggtacaca aagtaatatg
     6601 aatgtgaacg aagtagtaag ttatgttgct aatatgtatt taaaagatca tcaaagtgat
     6661 gaaagtatcc acccaaatga tgatgtaaat aaatcacaaa gttcgaatga tacattccca
     6721 actgctatgc acgttgcatt atatcaagag gttgaaacaa aattagaacc tgcattaaaa
     6781 cttttaagaa atactttgaa agaaaaagaa gacaaatttg attcaattat taaaattggt
     6841 cgtacacatt tacaagatgc aacgccgatc aaactaggac aagagattag tggctggcgt
     6901 tatatgcttg accgttgcga aataatgtta tctgaatcta agaagcacat tttaaatcta
     6961 gccattggtg gtacggctgt tggtactggt attaatgcgc atcctgaatt tggtgataaa
     7021 gtggcacagt atatttctga aaatacgggt tatccatttg tatcttctga aaataaattc
     7081 catgcattaa cagcacatga tgaagtagta caactacatg gaacattgaa ggcgctagca
     7141 ggagacttaa tgaaaattgc taatgatgtg agatggttgg cttcagggcc acgagctggt
     7201 ttggcagaaa tttctatccc tgaaaatgaa ccaggttcat caattatgcc tggtaaagtt
     7261 aatcctacac aatgtgaaat gttaacaatg gttgcagtcc aagtaatggg taatgataca
     7321 gttgttggct tcgcaagttc acaaggtaac tttgaattga atgtttataa accagttatt
     7381 atgcataata cactacaatc aatttacctt ttagctgatg gtatggaaac atttaataac
     7441 aattgtgcag tgggcattga accaatcgaa gagaatattg ataattattt aaatcaatca
     7501 ttaatgttag ttactgcatt aaatccacat attggttatg aaaaagctgc tcaaattgct
     7561 aagaaagccc ataaagaagg tttaacttta aaagaatctg caattcaaac tggatatgtt
     7621 acagaagaac aatttgaagc atggattaaa ccagaagata tggtagatcc tcattaatta
     7681 aatattaata taaaataaag tttgggcatt acaggtagat gtagtgttca agctttattt
     7741 ttgtctatgt caaattgaac ttataagtct gatttgatat agagccatga aaaggaaaaa
     7801 cttttcatgg agtccgtttt attatagaac ctatttttta tatgatgaat tgttgtacat
     7861 gtattggcca aacaacctgt gataaaaatc ttatgatgtc atgtattgtc catagataca
     7921 cgtaacgcag ttgaaaaaga ctacaagtca ttatccaagg atgttataaa atgaataatt
     7981 actaaaatat caaaatataa aatcttaagc atcgtaaaaa acaaaaaaac cgaacatctg
     8041 aaaaaattaa taattttcca tctatccgat ttaagttgat acttgattta gttacaatgc
     8101 ggtattgcat gtttaaatat catgcaaaac taatcaaatt agtcatccaa agaaacggaa
     8161 atgacttcct tagttaaggg atgcacaaac tctattttgt aactttcaag ttctaattgt
     8221 cttaacgttg aatcaccgta taaaggatca ccaataactg ggtgaccaat ttcagctaaa
     8281 tgcacacgaa tttgatgagt acgtccagta tccaacttga tttcaagttg gcacacagtt
     8341 tcttttatca tttttgaagt taaaatgtgt gtaattgcac gctgtccagt aggagacaca
     8401 cgtcgtttat tcgaatggaa tttatcttta ccaattggca tatcaatcgt ttgtggtttt
     8461 aaaggtaaaa gtgctttaac atttgctttg tatatccgcg taatatcatt gtcttctaac
     8521 atgcgatcaa gaattttttt cattaaagga tttttagcaa caattaataa tcctactgtt
     8581 tcctggtcca gtcgatgaat tggttcgaca taatcgctat caaccgtgta aatcacatga
     8641 ttcattaaag tattgctttc ttttaaatca ttagggtggg tcttaacacc tttaggtttc
     8701 attacgattg ccatatcatc gtcttcgtat ttaatttgtg cataacgata acttggaaca
     8761 taattacttt tttcatcggt ggttgggaca aatacatcgt cgccagtatg cactttatcc
     8821 attaatcgcg caggtttatc attaatagta atatctttag acatatttaa atgatgtaaa
     8881 tcttttttag gtaccttaag ttgttggaaa atatctcgta aacttaagtc attaaagttt
     8941 tctggtattt taaatttcat aaaatcctcc tatagtcttt aatatcatat cacaaatcag
     9001 ttctccgaaa tataactatt aagtaatcaa ggccatttta tcatgttcac gtctaagatt
     9061 taaaaatata ataactcaat gtataatagt taatacacgt aaaaatgaat tggagcgatt
     9121 tgatggaaca aaggacgcga ctagctttat taaaagagat tgccgaattt ttaaacgaag
     9181 aaactgaaat gtatagcatg acccaaggtg cgttaaaata tttaattgag ggcagtaatt
     9241 tcacgacagg ctggatattt ttcatcaata gcgtaggtga acatgaactt gtatcacatg
     9301 tggctttacc acaatctttg acagcagacc actgtcacta tattaaagat gggtcttgtt
     9361 ggtgtgtgaa agcatttaat caaggtaggc taatgaaagc gtcgaatatc gtcaattgtt
     9421 ctcgtattaa cttagcctct aaagcatttc ctagccaaaa tgacaatatt acacatcatg
     9481 caacggtacc acttaaatcg ggtcaagaac aattcggcat tttaaatgta gcgtcaccaa
     9541 atactgaaat atatagtgat gaagatttag agttgctaga gtctgtcgca tttcaattag
     9601 ggtcagccat caaacgtatt tatttaacag accgtgaaaa agaagcagca aaaattaatg
     9661 aacgtaatcg tttagctaga gatttacatg attcagtaaa tcaaatgtta ttttctgtaa
     9721 aactaacagc acatgctgcg tatggcgtgt caaacgaatc catcgctaaa caagcattca
     9781 agacgattga ggaaacgagt caaaatgccg ttaatgaaat gcgtgcattg atttggcaac
     9841 ttaagccagt tggacttgag caaggtttaa ttcatgcttt gacagcatat agtaaattaa
     9901 tgcatataca attaaatgta aatgtagaag gtttaatcga tttatctaat gaaatcgaag
     9961 aaaacatata cagagcatta caagagtgta ttaataatgt taagaaacat gctgatacga
    10021 ataaaatgga tcttacatta aaacaaatga acgatatact atatattgac gtgattgatt
    10081 atggtcaagg atttgagatc gataatgttc agatagcttc atcacacggt ataaataaca
    10141 taaaacaaag agttaaatta ttaagaggta aagtgacctt tcattctcag ccaacaaagg
    10201 gtacacaaat acaattcaca attcctataa aatagcttga tttaaggaga taacccatga
    10261 acaaagtaat attagtagat gaccattata ttgtgcgaca aggattgcga tttttattat
    10321 ccacgattga aaacatagaa gttttacaag actttgcaga tggagaaaca tttttagaat
    10381 atttaaaaga gcatgagcac cctgatattg tgctattaga tttagtgatg cctggcatga
    10441 atggtattga aattacggaa tatattaagg cacattatcc ggatataaaa gttttggtat
    10501 taacaagtta tgttgatgat gaacatgtaa tttcagcaat caataaaggt gctgatggtt
    10561 atgaaatgaa agacgttgag cctcagcaat taattgaaac tattagacga gttatgaacg
    10621 gtgaaaaaat gatacatcct aaggcacaag atgtattcga aacagttagc caaaaaccac
    10681 actacacgaa taagttgtca aagagagaaa ttgaagtgtt acgtgaaatg gttaaaggta
    10741 aaacaaataa agagattgca gaaactttat ttgtatctga aaaaacaatt aaaacacatg
    10801 tcagtcatat atttagtaaa ttacaagtta gcgatcgtac acaagcagca atttatgcaa
    10861 tggaaaataa gttgatttag aatgagcttt taaatatttg tcgaaaatta gacaattttt
    10921 aatctgattt tgatgaattt tttatagcga tagggtttat tatacagttt tctttgtaaa
    10981 tttgatatca ttataaaag
//
LOCUS       DAHHOJ010000091        10758 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.091, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000091 DAHHOJ010000000
VERSION     DAHHOJ010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10758)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10758)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10758
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.091"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            119..1648
                     /gene="aur"
                     /locus_tag="NBH32_002032"
     CDS             119..1648
                     /gene="aur"
                     /locus_tag="NBH32_002032"
                     /EC_number="3.4.24.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001225028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metalloproteinase aureolysin"
                     /protein_id="HCD1515614.1"
                     /translation="MRKFSRYAFTSMATVTLLSSLTPAALASDTNHKPATSDINFEIT
                     QKSDAVKALKELPKSENVKNHYQDYSVTDVKTDKKGFTHYTLQPSVDGVHAPDKEVKV
                     HADKSGKVVLINGDTDAKKVKPTNKVTLSKDEAADKAFNAVKIDKNKAKNLQDDVIKE
                     NKVEIDGDSNKYIYNIELITVTPEISHWKVKIDADTGAVVEKTNLVKEAAETGTGKGV
                     LGDTKNININSIDGGFSLEDLTHQGKLSAYNFNDQTGQATLITNEDENFVKDEQRAGV
                     DANYYAKQTYDYYKNTFGRESYDNHGSPIVSLTHVNHYGGQDNRNNAAWIGDKMIYGD
                     GDGRTFTNLSGANDVVAHELTHGVTQETANLEYKDQSGALNESFSDVFGYFVDDEDFL
                     MGEDVYTPGKEGDALRSMSNPEQFGQPSHMKDYVYTEKDNGGVHTNSGIPNKAAYNVI
                     QAIGKSKSEQIYYRALTEYLTSNSNFKDCKDALYQAAKDLYDEQTAEQVYEAWNEVGV
                     E"
     gene            2015..2464
                     /locus_tag="NBH32_002033"
     CDS             2015..2464
                     /locus_tag="NBH32_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HCD1515615.1"
                     /translation="MKKSKRLEIVSTIVKKHKIYKKEQIISYIEEYFGVRYSATTIAK
                     DLKELNIYRVPIDCETWIYKAINNQTEQEMREKFRHYCEHEVLSSIINGSYIIVKTSP
                     GFAQGINYFIDQLNIEEILGTVSGNDTTLILTASNDMAEYVYAKLFK"
     gene            2467..2634
                     /locus_tag="NBH32_002034"
     CDS             2467..2634
                     /locus_tag="NBH32_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515616.1"
                     /translation="MYQMNNENLFRITCTQVSYQILTCVRVFLWKERIHSHSFDCIKN
                     FIQHVFMGIFE"
     gene            2811..4046
                     /gene="arcA"
                     /locus_tag="NBH32_002035"
     CDS             2811..4046
                     /gene="arcA"
                     /locus_tag="NBH32_002035"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HCD1515617.1"
                     /translation="MTDGPIKVNSEIGALKTVLLKRPGKELENLVPDYLDGLLFDDIP
                     YLEVAQKEHDHFAQVLREEGVEVLYLEKLAAESIENPQVRSEFIDDVLAESKKTILGH
                     EEEIKALFATLSNQELVDKIMSGVRKEEINPKCTHLVEYMDDKYPFYLDPMPNLYFTR
                     DPQASIGHGITINRMFWRARRRESIFIQYIVKHHPRFKDANIPIWLDRDCPFNIEGGD
                     ELVLSKDVLAIGVSERTSAQAIEKLARRIFENPQATFKKVVAIEIPTSRTFMHLDTVF
                     TMIDYDKFTMHSAILKAEGNMNIFIIEYDDVNKDIAIKQSSHLKDTLEDVLGIDDIQF
                     IPTGNGDVIDGAREQWNDGSNTLCIRPGVVVTYDRNYVSNDLLRQKGIKVIEISGSEL
                     VRGRGGPRCMSQPLFREDI"
     gene            4079..5089
                     /gene="argF"
                     /locus_tag="NBH32_002036"
     CDS             4079..5089
                     /gene="argF"
                     /locus_tag="NBH32_002036"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1515618.1"
                     /translation="MTEIQKPYDLKGRSLLKESDFTKAEFEGLIDFAITLKEYKKNGI
                     KHHYLSGKNIALLFEKNSTRTRAAFTVASIDLGAHPEFLGKNDIQLGKKESVEDTAKV
                     LGRMFDGIEFRGFSQQAVEDLAKFSGVPVWNGLTDDWHPTQMLADFMTIKENFGYLEG
                     INLTYVGDGRNNIAHSLMVAGAMLGVNVRICTPKSLNPKEAYVDIAKEKASQYGGSVM
                     ITDNIAEAVENTDAIYTDVWVSMGEESEFEQRINLLKDYQVNQQMFDLTGKDSTIFLH
                     CLPAFHDTNTLYGQEIYEKNGLAEMEVTDQIFRSEHSKVFDQAENRMHTIKAVMAATL
                     GS"
     gene            5180..6610
                     /gene="arcD"
                     /locus_tag="NBH32_002037"
     CDS             5180..6610
                     /gene="arcD"
                     /locus_tag="NBH32_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HCD1515619.1"
                     /translation="MNESGDNKLSKSSLIGLVIGSMIGGGAFNIMSDMGGKAGGLAII
                     IGWIITAIGMISLAFVFQNLTNERPELDGGIYSYAQAGFGDFVGFISAWGYWFSAFLG
                     NVAYATLLMSAVGNFFPIFKGGNTLSSIIVASILLWGVHFLILKGVETAAFINSIVTV
                     AKLIPILLVIICMIIAFNFDTFKTGFFGMTSEGVLPFSWASTMSQVKSTMLVTVWVFI
                     GIEGAVIFSSRAKNKKDVGSATVIGLISVLIIYFLLTVLAQGVILQNHISQLDSPSMA
                     QVLATIVGGWGSTLVNIGLIISVLGAWLGWTLLAGELPFIVAKDGLFPKWFAKENKNG
                     APVNALLITNILVQLFLISMLFTQSAYQFAFSLASSAILYPYMFSAFYQVKYTLEHRQ
                     QATTKQWTIGIIASIYAIWLIYAAGINYLLLTMLLYIPALLVYSIVQKNNQTRLIKSD
                     YILFMIIIVLAVIGLIKLLMGTINVF"
     gene            6627..7568
                     /gene="arcC"
                     /locus_tag="NBH32_002038"
     CDS             6627..7568
                     /gene="arcC"
                     /locus_tag="NBH32_002038"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1515620.1"
                     /translation="MKEKIVIALGGNAIQTKEATAEAQQTAIRRAMQNLKPLFDSPAR
                     IVISHGNGPQIGSLLIQQAKSNSDTTPAMPLDTCGAMSQGMIGYWLETEINRILTEMN
                     SDRTVGTIVTRVEVDKDDPRFNNPTKPIGPFYTKEEVEELQKEQPDSVFKEDAGRGYR
                     KVVASPLPQSILEHQLIRTLADGKNIVIACGGGGIPVIKKENTYEGVEAVIDKDFASE
                     KLATLIEADTLMILTNVENVFINFNEPNQQQIDDIDVATLKKYAAQGKFAEGSMLPKI
                     EAAIRFVESGENKKVIITNLEQAYEALIGNKGTHIHM"
     gene            7667..8371
                     /locus_tag="NBH32_002039"
     CDS             7667..8371
                     /locus_tag="NBH32_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HCD1515621.1"
                     /translation="MTENFILGRNNKLEHELKALADYINIPYSILQPYQSECFVRHYT
                     KGQVIYFSPQESSNIYFLIEGNIIREHYNQNGDVYRYFNKEQVLFPISNLFHPKEVNE
                     LCTALTDCTVLGLPRELMAFLCKANDDIFLTLFALINDNEQQHMNYNMALTSKFAKDR
                     IIKLLCHLCQTVGYDQDEFYEIKQFLTIQLMSDMAGISRETAGHIIHELKDEKLVVKD
                     HKNWLVSKHLFNDVCV"
     gene            8721..>10758
                     /gene="clfB"
                     /locus_tag="NBH32_002040"
     CDS             8721..>10758
                     /gene="clfB"
                     /locus_tag="NBH32_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfB"
                     /protein_id="HCD1515622.1"
                     /translation="MKKRIDYLSNKQNKYSIRRFTVGTTSVIVGATILFGIGNHQAQA
                     SEQSNDTTQSSKNNASADSEKNNMIETPQLNTTANDTSDISANTNSANVDSTAKPMST
                     QTSNTTTTEPASTNETPQPTAIKDQATAAKMQDQTVPQEANSQVDNKTTNDANNIATN
                     SELKNPQTLDLPQSSPQTISNAQGTSKPSVRTRAVRSLAVAEPVVNAADAKGTNVNDK
                     VTASDFKLEKTTFDPNQSGNTFMAANFTVTDKVKSGDYFTAKLPDSLTGNGDVDYSNS
                     NNTMPIADIKSTNGDVVATATYNILTKTYTFVFTDYVNDKENINGQFSLPLFTDRAKA
                     PKSGTYDANINIADEMFNNKITYNYSSPIAGIDKPNGANISSQIIGVDTASGQNTYKQ
                     TVFVNPKQRVLGNTWVYIKGYQDKIEESSGKVSATDTKLRIFEVNDTSKLSDSYYADP
                     NDSNLKEVTNEFKDKITYKYQNVASINFGDITKTYVVLVEGHYDNTGKNLKTQVIQEN
                     IDPATGKDYSIFGWNNENVVRYGGGSADGDSAVNPVDPTPGPPVDPEPSPEPEPEPTP
                     DPEPSPDPEPEPTPDPEPSPDPEPEPTPDPEPSPDPDSDSDSDSDSDSNSDLDSDSGS
                     DSDSDSDSDSDSDSDSDLDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD"
BASE COUNT         3979 a         1585 c         2067 g         3127 t
ORIGIN      
        1 aaataaaatc aacgaacaaa aagtatagaa ataaatagaa ataatcgttt acttttctga
       61 aaaattaaat taatatttta tttataagct gtttttaaaa tttcaggagg aatgaaatgt
      121 gaggaaattt tcaagatatg catttacaag tatggcgaca gtaacgttgc tgagctcttt
      181 gacacctgca gcactagcga gtgatacgaa tcacaaacca gcaacttcag atattaattt
      241 tgaaatcaca caaaagagtg atgcagttaa agcattaaaa gagttaccta aatctgaaaa
      301 tgtgaaaaat cattatcaag attactctgt tacagatgta aaaacagata agaaaggatt
      361 tacgcattac acgttacaac cgagtgtgga tggtgttcat gcacctgaca aagaagtgaa
      421 agtacacgca gacaaatcag gaaaagtcgt tttaatcaac ggtgatactg acgcaaagaa
      481 agtaaagccg acaaataaag tgacattaag caaggatgaa gcggctgaca aagcatttaa
      541 cgcagttaag attgataaaa ataaagctaa aaacctccaa gatgacgtta tcaaagaaaa
      601 taaagtcgaa atcgacggtg acagtaataa atacatttac aatattgaat taattacagt
      661 aacaccagaa atttcacatt ggaaagttaa aattgatgcc gacacaggag cagttgttga
      721 aaaaacgaac ttagttaaag aagcggcaga aactggtaca ggtaaaggag tactcggcga
      781 tacaaaaaat atcaatatca atagtattga cggtggattt agcctagaag atttaacgca
      841 tcaaggtaaa ttatcagcat acaattttaa tgatcaaaca ggtcaagcaa cattgattac
      901 taatgaagat gaaaacttcg tcaaagatga gcaacgtgct ggtgtagatg cgaattatta
      961 tgctaaacaa acatatgatt actacaaaaa tacatttggt cgtgagtctt acgataacca
     1021 tggtagtcca attgtctcat taacacatgt aaatcattat ggtggacaag ataacagaaa
     1081 taacgctgca tggattggag acaaaatgat ttatggtgat ggcgatggcc gcacgtttac
     1141 aaatttatca ggtgcaaatg acgtagtagc acatgagtta acacatggcg tgacacaaga
     1201 aacggcgaat ttagagtata aagatcaatc tggtgcgtta aatgaaagct tttcagatgt
     1261 ttttggatac tttgtagatg atgaggattt cttgatgggt gaagatgttt acacaccagg
     1321 aaaagaggga gatgctttac gaagcatgtc aaacccagaa caatttggtc aaccatctca
     1381 tatgaaagac tatgtataca ctgaaaaaga taacggtggt gtgcatacga attctggcat
     1441 tccaaataaa gcagcttata acgtaattca agcaataggg aaatctaaat cagaacaaat
     1501 ttactaccga gcattaacgg aatacttaac aagtaattca aacttcaaag attgtaaaga
     1561 tgcattatac caagcggcta aagatttata tgacgagcaa acagctgaac aggtgtatga
     1621 agcatggaat gaagtaggcg tggagtaaaa atatataaac aagaagaagt aatgttaaac
     1681 acttataaat aattaaattt taaatacagc ttatatatgg ggtataccta cattgataat
     1741 tgactttgta ggtataccct ctctttgtaa ttagatgaga ggagtgtgag ggttgtctgc
     1801 cgaaagacta ctcggcagtc taaaatcatt acaagtagta gatatgtgat aattaaatgc
     1861 tgacttagaa tacaaaattc attttaaaag ttgtcacaaa aaatttacat gtatttttat
     1921 tatcttttgc aaaacaaagt gttaaattat aaatgaaaca tgcatgaatt tattttttaa
     1981 tacaagaaac gtaactacca aaggagttta caatatgaag aaaagtaaac gattagaaat
     2041 tgtttctaca atagttaaaa agcataagat ttataaaaaa gaacaaatca tttcatatat
     2101 tgaagaatat tttggtgtaa gatatagtgc aacaacaatt gctaaagact tgaaagaact
     2161 taatatatat cgtgtcccta tcgattgtga aacatggatt tataaagcta ttaataatca
     2221 aacagaacaa gagatgagag aaaagtttag acactattgt gaacatgaag ttctaagttc
     2281 aatcatcaat ggttcataca ttatcgttaa aacctcacct ggtttcgccc aaggcataaa
     2341 ctattttatc gatcagctaa atatagaaga gatattaggt acggtgagtg gaaatgacac
     2401 tacattaatc ttaactgcct caaatgatat ggcagaatac gtatatgcaa aattatttaa
     2461 atagacatgt atcaaatgaa taatgaaaat ttgtttcgta tcacgtgtac tcaagttagt
     2521 taccaaatat taacttgtgt acgcgttttt ttatggaaag aaagaattca tagtcattca
     2581 tttgactgta taaaaaactt tatacaacat gtttttatgg gtatttttga ataaaaaatg
     2641 tatatttcga cccaaaacac ctttatttat gtataaaaat ccattattat gtattgtata
     2701 acaaaaagat atgaaatttt cgactttctt tatgtgaata taatcacatg taagcgtttg
     2761 aagattgtct atactctaaa tgaattcaaa gataaaagga ggaaatagac atgacagatg
     2821 gtccaattaa agtaaatagc gaaattggag ctttaaaaac tgtgttactt aagcgtcctg
     2881 gaaaagaatt agaaaattta gtacctgatt atttagatgg attactattt gatgatattc
     2941 catatttaga agtagctcaa aaagagcatg accattttgc acaggtgcta agagaagagg
     3001 gtgttgaagt actttacctt gagaagttag cagctgaaag tattgaaaat cctcaagtaa
     3061 gaagtgaatt tattgatgat gtattagcag agtctaaaaa aacaatatta ggtcatgaag
     3121 aagaaattaa ggcattattt gcgacacttt ctaatcaaga acttgtagat aaaataatgt
     3181 caggggtacg taaggaagaa attaatccga aatgtacaca tctagtagag tatatggatg
     3241 ataagtatcc attctattta gatccaatgc caaaccttta ttttactaga gatccacaag
     3301 cctcaatagg acacggtata acaatcaatc ggatgttctg gagagcacga cgacgagaat
     3361 caatatttat tcaatatatt gtaaagcatc atcctagatt taaagatgcg aatattccaa
     3421 tctggttaga tcgagattgc ccattcaata ttgaaggcgg cgatgaactt gttttatcta
     3481 aagatgtctt ggctataggc gtttcagaac gtacatctgc acaagctatt gaaaagttag
     3541 cgcgacgtat ttttgaaaat ccgcaggcga cgtttaaaaa agtagtagca attgaaattc
     3601 caactagtcg aacttttatg cacttagata cagtatttac aatgatagat tatgacaaat
     3661 ttacaatgca ttcagccatt ttaaaggcag aaggcaatat gaatatattt attattgaat
     3721 atgatgacgt aaataaagat attgccatca aacaatctag tcatttaaaa gatactttag
     3781 aagacgtact aggtatagat gatatccaat tcattccaac aggaaatggt gatgtcattg
     3841 atggtgctag agagcaatgg aatgatggct caaatacatt atgtataaga ccaggcgttg
     3901 tagtgactta cgatagaaac tatgtatcga atgatttatt gagacaaaaa ggcattaaag
     3961 tcattgaaat atctggtagc gagttggtac gtggacgtgg gggtcctaga tgtatgagtc
     4021 aaccactatt cagagaagac atttaacgta ataaattata gaaacgaggg tgaaaataat
     4081 gacagaaatt caaaaaccgt atgatttaaa aggcagatca ttattaaaag aaagtgattt
     4141 taccaaagca gaattcgaag gacttattga ttttgcaatt acattaaaag agtataagaa
     4201 aaacggtatt aagcatcact acttatctgg aaaaaatatt gcactactat tcgaaaagaa
     4261 ttcgacgaga acgcgtgcag cgtttacagt tgcgtctatt gatttaggtg cgcatccaga
     4321 atttttaggg aaaaatgata ttcaattagg caaaaaagaa tctgtagagg atactgcgaa
     4381 agtattaggt agaatgttcg atggtattga attccgtggt ttttcacaac aagctgttga
     4441 agatttagcg aagttctctg gtgtaccggt gtggaatgga ttaacagacg attggcatcc
     4501 tacacaaatg ttagctgatt ttatgacaat aaaagagaat tttggatatc tagaaggaat
     4561 aaacttaact tacgttggag atggacgtaa taatattgcg cattcattaa tggtagcagg
     4621 tgctatgtta ggtgttaatg taagaatttg tacacctaaa tcattaaatc caaaagaggc
     4681 atatgttgat attgcaaaag aaaaagcgag tcaatatggt ggttcagtca tgattacgga
     4741 taatattgca gaagcagttg aaaatacaga tgctatatat acagatgttt gggtatcgat
     4801 gggtgaagaa agtgaatttg aacaacgtat taatttatta aaagactatc aagtgaatca
     4861 acagatgttt gatttaacag gtaaagattc aacgatattc ttacattgtt taccagcatt
     4921 ccatgataca aatacacttt atggacaaga aatttatgaa aaaaatggat tagctgaaat
     4981 ggaagttaca gaccaaatct ttagaagtga acattcaaaa gtgtttgatc aagctgaaaa
     5041 tagaatgcat acaattaagg cagtaatggc agcaacattg gggagttaat cactaaatgg
     5101 aatgatatga atatgatgtg tctgatgata taagtgtcat gtacagacac ctcatattgg
     5161 tattaaagga gaaatgaata tgaacgaatc aggagataac aaactcagta aatcttcttt
     5221 aattggacta gttataggat ccatgattgg tggcggtgcg ttcaatataa tgtctgatat
     5281 gggcggtaaa gccggtggat tagccattat tattggttgg attattacag ctataggaat
     5341 gatttcatta gcgttcgtat ttcaaaattt aaccaatgaa cggccggagc tagacggtgg
     5401 tatttatagt tatgctcaag caggatttgg cgattttgta ggctttatca gtgcttgggg
     5461 atattggttc tcagcgtttt taggcaatgt tgcctatgca acactattga tgtcagcagt
     5521 aggtaacttt ttcccgattt ttaaaggagg caacacatta tcaagtatta ttgtcgcatc
     5581 tatattactt tggggtgtgc atttcctaat tttaaaaggt gttgaaacag cagcctttat
     5641 caatagtatt gttactgttg caaagttaat accgatttta cttgtaatca tatgcatgat
     5701 aattgcattc aattttgaca cttttaaaac aggctttttc ggtatgacgt cagagggtgt
     5761 attgccattt agttgggcga gcacaatgag ccaagttaaa agtacgatgc tagtgacagt
     5821 ttgggtgttt atcggtatcg aaggtgcagt aattttttct agtagagcta aaaataaaaa
     5881 agatgtaggt agtgccacgg ttataggact tatatcagtt ttaattatct atttcttatt
     5941 aactgtatta gctcaaggcg tgattttgca aaatcatatt tctcaattag attcgccaag
     6001 tatggcacag gtgcttgcaa ctattgtagg tggttgggga tctacacttg taaatattgg
     6061 tttaattatt tcggtactag gtgcatggtt aggatggaca ttgcttgctg gtgaattacc
     6121 tttcattgtt gcaaaagatg gattatttcc aaaatggttt gctaaagaaa ataaaaatgg
     6181 agcacctgta aatgcactgc ttattaccaa tatattagta caattatttt taataagtat
     6241 gctatttaca cagagtgcgt atcaatttgc attttcacta gcatcaagtg ctattttata
     6301 cccttacatg ttcagtgcat tttaccaagt taaatacact ttagagcatc gacagcaagc
     6361 aactactaaa caatggacga ttggtatcat agcctcaatt tatgctatat ggcttatata
     6421 tgcagcaggt atcaattact tattattgac gatgttactt tatattccag ctcttcttgt
     6481 ttattcaatc gttcaaaaga ataatcagac acgtttgatt aaatcagact atatcctttt
     6541 tatgattatt atcgtacttg cagttatcgg gttaattaag ttattgatgg gaacgataaa
     6601 tgttttttaa aaggagcgac aaaaatatga aagagaaaat tgtcattgca ttaggcggta
     6661 atgcgataca gacaaaagaa gcaacagctg aagcacaaca aacagctatt agacgtgcga
     6721 tgcaaaacct taaaccttta tttgattcac cagcgcgtat tgtcatttca catggtaatg
     6781 gcccacaaat tggaagttta ttaatccaac aagctaaatc gaacagtgac acaacgccgg
     6841 caatgccatt ggatacttgt ggtgcaatgt cacagggtat gataggctat tggttggaaa
     6901 ctgaaatcaa tcgcatttta actgaaatga atagtgatag aactgtaggc acaatcgtta
     6961 cacgtgtgga agtagataaa gatgatccac gattcaataa cccaaccaaa ccaattggtc
     7021 ctttttatac gaaagaagaa gttgaagaat tacaaaaaga acagccagac tcagtcttta
     7081 aagaagatgc aggacgtggt tatagaaaag ttgttgcgtc accactacct caatctatac
     7141 tagaacacca gttaattcga actttagcag acggtaaaaa tattgtcatt gcatgcggtg
     7201 gtggcggtat tccagttata aaaaaagaaa atacctatga aggtgttgaa gcggttatag
     7261 ataaagattt tgctagtgag aaattagcaa cgctgattga agcagatacc ttaatgattc
     7321 ttacgaatgt agaaaatgta tttattaact ttaatgaacc taatcaacaa caaatcgatg
     7381 atattgatgt agcaacactg aaaaaatacg cggcacaagg taagtttgcg gaaggatcga
     7441 tgttgccaaa aatagaagct gcgatacgat ttgttgaaag tggggaaaac aaaaaagtta
     7501 tcattaccaa tttagagcag gcatacgaag ctttgattgg taataaaggt acacacattc
     7561 acatgtagca ctttatcacg cgacaaaaca ttaaatatgt ttctccgttg attcaaatga
     7621 aaaagttgtc tgctgacact ttgcaaggtt tgaaggagtt taacttatga cagaaaactt
     7681 tattttgggt agaaataata aattagaaca tgaactaaag gcattagcag attacattaa
     7741 tatcccatat agtatattac aaccatatca aagtgaatgt tttgtcagac attatacgaa
     7801 aggccaagtt atttattttt cgccacaaga aagtagcaat atttactttt taattgaagg
     7861 taacattatt agagaacatt acaatcaaaa tggagatgta tatcgttatt ttaataaaga
     7921 gcaagtatta tttccaatca gtaacttatt tcatccgaaa gaggttaacg aattgtgtac
     7981 agcactaacc gattgtacag ttcttggatt gcctagagaa ttgatggcct ttttgtgcaa
     8041 agctaatgat gatatatttt tgacactttt tgcattaata aatgataatg agcagcaaca
     8101 catgaactat aacatggcat taacaagtaa atttgctaaa gatcgaatta tcaaattgtt
     8161 atgccatcta tgtcagacag taggatacga tcaagatgaa ttttatgaaa tcaaacagtt
     8221 tttaactatt caactcatga gtgatatggc tgggatttcc cgggaaacag ctggtcatat
     8281 tattcatgaa cttaaagatg aaaaacttgt tgttaaagat cataaaaatt ggttagtaag
     8341 caaacattta ttcaatgatg tatgtgttta atatacaatg taaaatgaat aagttgaaca
     8401 tgaggtctaa cgtacattta tacgttaggc cttttttgct agcatgatga ttaatttaat
     8461 atgttagtta aatttgattg ttgaaattac agtaaaattt aaggtgatga aaaatttaga
     8521 acttctaagt ttttgaaaag taaaacattt gcaatagtgt aaaaatagta tattgatttt
     8581 tgctagttaa cagaaaattt taagttatat aaataggaag aaaacaaatt ttacgtaatt
     8641 tttttcgaaa agcaattgat ataattctta tttcattata caatttagac taatctagaa
     8701 attgaaatgg agtaatattt ttgaaaaaaa gaattgatta tttgtcgaat aagcagaata
     8761 agtattcgat tagacgtttt acagtaggta ccacatcagt aatagtaggg gcaactatac
     8821 tatttgggat aggcaatcat caagcacaag cttcagaaca atcgaacgat acaacgcaat
     8881 cttcgaaaaa taatgcaagt gcagattccg aaaaaaacaa tatgatagaa acacctcaat
     8941 taaatacaac ggctaatgat acatctgata ttagtgcaaa cacaaacagt gcgaatgtag
     9001 atagcacagc aaaaccaatg tctacacaaa cgagcaatac cactacaaca gagccagctt
     9061 caacaaatga aacacctcaa ccgacggcaa ttaaagatca agcaactgct gcaaaaatgc
     9121 aagatcaaac tgttcctcaa gaagcaaatt ctcaagtaga taataaaaca acgaatgatg
     9181 ctaataacat agcaacaaac agtgagctta aaaatcctca aacattagat ttaccacaat
     9241 catcaccaca aacgatttcc aatgcgcaag gaactagtaa accaagtgtt agaacgagag
     9301 ctgtacgtag tcttgcagtt gctgaacctg tagtaaatgc tgctgatgct aaaggtacaa
     9361 atgtaaatga taaagttacg gcaagtgatt tcaagttaga aaagactaca ttcgacccta
     9421 accaaagtgg taacacattt atggcggcaa attttacagt gacagataaa gtgaaatcag
     9481 gggattattt tacagcgaag ttaccagata gtttaactgg taatggagac gtggattact
     9541 ctaattcaaa taatacgatg ccaattgcag acattaaaag tacgaatggt gatgttgtag
     9601 cgacagcgac ttataatatc ttgactaaga cgtatacatt tgtctttaca gattatgtaa
     9661 atgataaaga aaatattaac ggacaatttt cattaccttt atttacagac cgagcaaagg
     9721 cacctaaatc aggaacatat gatgcaaata ttaatattgc ggatgaaatg tttaataata
     9781 aaattactta taactatagt tcgccaattg caggaattga taagccaaat ggcgcgaaca
     9841 tttcttctca aattattggt gtagatacag cttcaggcca aaacacatac aagcaaacgg
     9901 tatttgttaa ccctaagcaa cgagttttag gtaatacgtg ggtgtatatt aaaggctacc
     9961 aagataaaat cgaagaaagt agcggtaaag taagtgctac agatacaaaa ctgagaattt
    10021 ttgaagtgaa tgatacatct aaattatcag atagctacta tgcggaccca aatgactcta
    10081 atcttaaaga agtaacgaat gagtttaagg ataaaatcac ttataaatac caaaatgtag
    10141 caagtattaa ttttggcgat attactaaaa cgtatgttgt attagtggaa ggtcactatg
    10201 ataatactgg taaaaacttg aaaacacagg ttattcaaga aaatattgac ccagcgacag
    10261 gtaaagatta cagtattttc ggttggaata atgagaatgt tgtacgttat ggaggcggaa
    10321 gtgctgatgg tgattcagca gtaaatccag ttgatccaac accgggacca ccagttgacc
    10381 cagagccaag tccagagcca gaaccggaac caacgccgga tccagagcca agtccagacc
    10441 cagagccgga accaacaccg gacccagaac caagtccaga cccagagccg gaaccaacac
    10501 cggatccaga gccaagtcca gacccggatt cggattcgga cagtgactca gattcggata
    10561 gcaactcgga cttagacagt gactcgggct cagacagtga ttcagactca gatagcgatt
    10621 cagattcaga tagcgactca gacttagaca gtgattcaga ttcagatagc gactcagact
    10681 cagacagtga ttcagactca gatagcgatt cagactcgga tagcgattca gactcagaca
    10741 gcgattcaga ttcggata
//
LOCUS       DAHHOJ010000092        10360 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.092, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000092 DAHHOJ010000000
VERSION     DAHHOJ010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10360
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.092"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            225..479
                     /locus_tag="NBH32_002041"
     CDS             225..479
                     /locus_tag="NBH32_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000466745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCD1515623.1"
                     /translation="MFGFIGMLIVGGLIGWAAGAIMGKDIPGGILGNIIAGIIGSWLG
                     GTLLGHWGPELGSIYILPALIGSIILIALVTLILRAVRKK"
     gene            complement(518..726)
                     /locus_tag="NBH32_002042"
                     /pseudo
     CDS             complement(518..726)
                     /locus_tag="NBH32_002042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498948.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            904..1485
                     /locus_tag="NBH32_002043"
     CDS             904..1485
                     /locus_tag="NBH32_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1515624.1"
                     /translation="MTIYLVRHGESQSNYDNKHFRSYFCGQLDVPLTDFGKKSADDLC
                     DYFKEKQIKHVYVSDLLRTQQTFEHIFPYDIASTTTPLLRERSLGVFEGEYKDEISAN
                     PKYEKYFNDPNFKDFRHSFSQKAPEGESYEDVYQRVEHFMNHVVNEDTQKDDIVIVAH
                     QVVIRCLMVYFNNVSREEAVDLKVENCKPYIIE"
     gene            complement(1551..1934)
                     /locus_tag="NBH32_002044"
     CDS             complement(1551..1934)
                     /locus_tag="NBH32_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515625.1"
                     /translation="MKVRITFIILAILSTIVCLFLAAMHPTGPNTVTFEQPYLFTLNI
                     IIMVLVALPSLVLAIYDYMSFRISSAILQFLGAISWFFLSLILSFTQFTPFTLASFIT
                     SIILFASTIITLAIGGKSVDKNDSL"
     gene            complement(2189..2815)
                     /locus_tag="NBH32_002045"
     CDS             complement(2189..2815)
                     /locus_tag="NBH32_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NDxxF motif lipoprotein"
                     /protein_id="HCD1515626.1"
                     /translation="MKKRLLLSTFLASALILTGCASDQSDNEDHHTSTGIHAPKSAKK
                     LETKDIFNSDKKNSDISDAEMKQAIEKYLSVNSDILDNKYIMQHKLDKQIDSQTKVTE
                     KQAETLSHLSNLAVKNDLHFKKFVTENNIPKEYKKPVELMMNYFKALNSTIANVDEDI
                     EKLSYQPQNKINVVDVPTKYAGDVNKKQQDKIKDFLKSKGIKSDVIDK"
     gene            complement(2888..4510)
                     /locus_tag="NBH32_002046"
     CDS             complement(2888..4510)
                     /locus_tag="NBH32_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515627.1"
                     /translation="MLDAHFIYPGKLQNACEYFNLPNDKEGFEVTMHALRPENLHKIY
                     HDMNKTEQKFIETLISKGNPVTIINSHEVNILKLTNHLGFVIQINEDTFFVNNLFLSY
                     INSEVLNSAYGEFETLDTKDHMHLNDEIFRVRQHTRLNDVTMEQALLLKTNDELKAIC
                     RQYGIKGFSNKNKQNLVKLIMTAFFNNNHIIEQVFSNASLFEFSILTGILESDDNYRL
                     NNELITFENIHLMNPGTFLITHFYYYYDFGYDCLILPNDVRKHIENYLNQIADGDIKE
                     AINEFIDEQTIKHSLADFENAIELSMMDDEVHFDILYDEDDDFEDFGTTDSLRNDAIT
                     ELISEIKKGLIDKKDILPLRIILGSVNLYGILSTNHLLYLTQRFYDATFSKEELQYSL
                     DTLAGTELYYIEDDFVFHPVLFDVVESEASGINISDEGYYVPASVEELIYYDRHHYLK
                     SDSEIKAFTSYLRKQINMKNDIEKEKYVNEIIMLLRTIPTSKLIQPIMQLFIDKAILN
                     NVQNDALLIEKATHARNHLRLWSLQGHRDIPN"
     gene            complement(4625..6148)
                     /gene="ahpF"
                     /locus_tag="NBH32_002047"
     CDS             complement(4625..6148)
                     /gene="ahpF"
                     /locus_tag="NBH32_002047"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCD1515628.1"
                     /translation="MLNADLKQQLKQLLELMEGNVEFVASLGSDDKSKELKELLTEIS
                     DMSPRLSLSEKSLKRTPSFSVNRPGEETGITFAGIPLGHEFNSLVLAILQVSGRAPKE
                     KQSIIDQIKNLEGSFHFETFISLTCQKCPDVVQALNLMSVINPNITHSMIDGAVFREE
                     SENIMAVPAVFLNGEEFGNGRMTIQDILSKLGSTADASEFENKEPYDVLIVGGGPASG
                     SAAIYTARKGLRTSIVADRIGGQVNDTAGIENFITVKETTGSEFSSNLAAHIDQYDID
                     AMTGIRATDIEKTDEAIKVTLENGAVLESKTVIIATGAGWRKLNIPGEEQLINKGVAF
                     CPHCDGPLFENKDVAVIGGGNSGVEAAIDLAGIVNHVTLFEFASELKADNVLQDRLRS
                     LSNVDIKTNAKTTEVVGEDHVTGIRYEDMSTGEEHLLNLDGIFVQIGLLPNTSWLKDA
                     VELNERGEIVIDRNNNTNVPGIFAAGDVTDQKNKQIIISMGAGANAALNAFDYIIRN"
     gene            complement(6164..6733)
                     /gene="ahpC"
                     /locus_tag="NBH32_002048"
     CDS             complement(6164..6733)
                     /gene="ahpC"
                     /locus_tag="NBH32_002048"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002445177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HCD1515629.1"
                     /translation="MSLINKEILPFTAQAFDPKKDQFKEVTQEDLKGSWSVVCFYPAD
                     FSFVCPTELEDLQNQYEELQKLGVNVFSVSTDTHFVHKAWHDHSDAISKITYTMIGDP
                     SQTITRNFDVLDEATGLAQRGTFIIDPDGVVQASEINADGIGRDASTLAHKIKAAQYV
                     RKNPGEVCPAKWEEGAKTLQPGLDLVGKI"
     gene            7225..7980
                     /gene="nfsA"
                     /locus_tag="NBH32_002049"
     CDS             7225..7980
                     /gene="nfsA"
                     /locus_tag="NBH32_002049"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NADPH nitroreductase"
                     /protein_id="HCD1515630.1"
                     /translation="MSDHVYNLVKKHHSVRKFKNKPLSEDVVKKLVEAGQSASTSSFL
                     QAYSIIGIDDEKIKENLREVSGQPYVVENGYLFVFVIDYYRHHLVDQHAETDMENAYG
                     STEGLLVGAIDAALVAENIAVTAEDMGYGIVFLGSLRNDVERVREILDLPDYVFPIFG
                     MAVGEPADDENGAAKPRLPFDHVFHHNKYHADKETQYAQMADYDQTISEYYDQRTNGN
                     RKETWSQQIEMFLGNKARLDMLEQLQKSGLIQR"
     gene            complement(8060..9448)
                     /locus_tag="NBH32_002050"
     CDS             complement(8060..9448)
                     /locus_tag="NBH32_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HCD1515631.1"
                     /translation="MNAFLTLINIIVLVIFIVILHMMARKHISFAKRVFTALGIGIVF
                     GVLLHLVYGTHSNVITSTSDWFNIVGQGYVALLQMIVMPLIFISIVAAFTKIQIGEKF
                     AKIGSLIFIFLIGTVTIAAIVGVVYALVFGLDASTINLGNAEQARGSEIAKQAKDLTA
                     HTLPQQILELLPKNPFLDFTGQRATSTIAVVIFASFIGFAYLRVARKQPDHGELLKRA
                     IDAIYSLVMAIVTFVLRLTPYGVLAIMANTLSTSDFGAIWTLGKFLIASYAALITMYI
                     IHLIILSLLGISPIRYIKKTLEVLIFAFTSRSSAGALPLNVQTQTRRLGVPEGIANFA
                     ATFGLSIGQNGCAGIYPAMLAIMVAPVANVEIDLQFIVTLIAVVIISSFGVAGVGGGA
                     TFASILVLSTLNLPVALAGVLISVEPLIDMGRTALNVNDSMLAGTGTAKLTKHWDKDT
                     FESNDNAALTSH"
     gene            9533..9631
                     /locus_tag="NBH32_002051"
     CDS             9533..9631
                     /locus_tag="NBH32_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515632.1"
                     /translation="MQLNVNMMKFDRWHKGICKQIVLNLVEKGEKG"
BASE COUNT         3483 a         1615 c         1845 g         3417 t
ORIGIN      
        1 ataacataaa ataatcaagc tatgaattcg ccagaaaaaa taatgtatat gatattgccg
       61 gtacagagac tttaaataac gatagctaca ttgaataaaa ttgatattca attactactt
      121 tgaaaaatat ttagataaaa ataatttgaa tttgttttag aaagtctaat aacgggtact
      181 acttagaata acgcaaaata aatagaaaaa ggagactgaa aattatgttt ggatttattg
      241 gaatgttaat tgtcggtggc ttaattggat gggctgctgg tgctattatg ggtaaagata
      301 tcccaggtgg tattttaggt aatattatcg caggtattat tggatcatgg ttaggtggaa
      361 cattattagg acattggggt cctgaactag gaagcattta catcttacca gctttaattg
      421 gttcaattat cttaattgca ctggtaacgc taattttaag agctgtgcgt aaaaaataaa
      481 atattcaact taggttgaat ggaaatcaag atggcattca gttgtcatct tttttatttg
      541 cgtgaaaatg gtcaatatgt attataatat ttgaaaatgt aggtaagata ggattaaaaa
      601 gtaataaatt aacatgagga aaggttggaa ataaaaaagc gagtatttga caaataaaaa
      661 aggatgacgt tgggttgcgc catccaggag tattatgttc atgaatataa aagactgtgt
      721 agtcatttaa gaactacacc catatagtaa cacatcactt gattaaagac aatactattt
      781 ttagagtcat taatttaaaa tatgaattaa ttcttttttt agaatagaat attaagattg
      841 atattgaatt agtagtcaaa gtgttatggt agatatgaaa tacataatgt aaggagtaat
      901 attatgacga tttatttagt tagacatggc gaatcacaat cgaattatga taataaacat
      961 tttcgatctt atttttgtgg acaattagat gtgccgttaa cggattttgg taagaaaagt
     1021 gcagacgatt tatgtgatta ttttaaagag aaacagatta aacatgtata tgtttcagac
     1081 ttattaagga cacagcaaac gtttgaacat atttttccat atgacattgc atcaacgact
     1141 acgcctctat taagagaacg ttcgcttggc gtatttgagg gtgaatataa agatgaaatc
     1201 agtgcgaatc cgaaatatga aaaatatttc aatgatccaa actttaaaga ctttcgtcat
     1261 agtttttcac aaaaagcgcc tgaaggagaa agttatgaag atgtatatca acgcgtcgaa
     1321 cattttatga atcatgttgt caatgaagat acacaaaaag atgatatcgt cattgttgca
     1381 catcaagttg tcattcgttg tttaatggtt tattttaata acgtttcacg ggaagaagct
     1441 gtggatttaa aagttgaaaa ttgcaaacca tatatcattg aatagaagta tagaggttct
     1501 gaaaagctgt gttttatagc ggccttcaga accttttttg tatttggaat ttaaagggaa
     1561 tcattcttat caacagactt accaccaata gctaatgtga taattgtgct tgcgaacaaa
     1621 ataattgaag ttataaatga cgctaatgta aaaggtgtaa attgtgtgaa cgataatatc
     1681 aatgataaaa agaaccaaga gatagcccct agaaattgta aaatagcaga agaaattcta
     1741 aaactcatat aatcatatat agctaaaaca agtgaaggta atgcgactaa taccattata
     1801 atgatgttga gggtgaataa atatggctgt tcaaaagtta ctgtgtttgg tcctgttgga
     1861 tgcattgctg ctaagaacaa gcaaacaatc gttgataaaa ttgctaaaat aataaaagta
     1921 attcgaactt tcatcatgac catcctttgt ttatagagtc aatataatta tgaaatacgt
     1981 tagagatata gtcaaatgcg tcaactaatg gggattttag gatggataga gaatttaagg
     2041 caattaaaaa agcatcaaac agtaatatgc tgcttgatgc ccaaatgatg actttagcta
     2101 aattgattag tcacttttaa ggataaagaa ttgccatgag ttaaagcaca tgtaatgatg
     2161 tgttacatgt cgcaatgatg gcattcagtt atttatcgat aacatcactc ttgatacctt
     2221 tagattttaa gaaatcttta attttatctt gttgcttttt attaacatca ccggcatatt
     2281 ttgttggcac gtcgactaca ttgattttat tttgcggttg atagctaagc ttttcaatat
     2341 cttcatcaac attggcgatt gtactattta aagctttgaa gtaattcatc attaattcaa
     2401 cgggtttctt atattcttta ggaatattgt tttcagtgac aaatttcttg aaatgcaaat
     2461 cattttttac agctaagtta gataagtgac taagtgtttc tgcttgtttt tcagtcactt
     2521 ttgtttgact atcaatttgt ttatctagtt tatgttgcat tatatatttg ttatcaagta
     2581 tatcgctatt tacagacaaa tacttttcta tagcttgctt catttctgca tcactaatat
     2641 cactattttt cttatctgag ttaaagatat cttttgtttc taatttttta gcgcttttag
     2701 gtgcatggat gccagtactt gtgtgatgat cttcgttatc agattgatcg gacgcgcaac
     2761 ctgtaagaat taatgcagat gctaaaaatg tacttagtag taatctcttt ttcataatgt
     2821 aatataactc cttggtttat ctttaattga aaaaatgtgt attcatgttt aatagagtaa
     2881 cattgaatta gtttggaatg tcacgatgac cttgcaatga ccatagacgt aaatgattac
     2941 gtgcatgagt tgctttttct atcaataatg catcattttg gacgttgtta aggatagctt
     3001 tatctataaa taactgcata attggttgta tcaatttaga tgtaggtatt gtacgtaaaa
     3061 gcataatgat ttcattcaca tatttttcct tctcaatatc atttttcata ttgatttgtt
     3121 tgcgtagata acttgtgaac gctttaattt ctgaatcaga tttcaaatag tggtgtctat
     3181 cataatagat taattcctca acagaagctg gtacatagta cccttcatca gaaatattaa
     3241 tgcctgacgc ctcagactcg acaacatcaa ataacacagg gtgaaacacg aagtcatctt
     3301 caatataata tagctcagta ccagctaaag tatctaagct atattgcaac tcttctttac
     3361 taaaagtagc gtcataaaat ctttgtgtga gatatagtag atgattagtt gataaaatac
     3421 catacaagtt tacagaacca agtataatac gaagaggtaa aatatctttt ttatctatta
     3481 atcctttctt aatctcagaa atcaattcag tgattgcatc atttcttaaa ctatcggttg
     3541 taccaaaatc ttcaaagtca tcatcttcat catatagtat atcgaagtga acttcgtcat
     3601 ccatcattga taattctatc gcattttcaa aatctgctaa actatgctta atcgtttgct
     3661 catcaataaa ttcattgata gcctctttta tatcgccatc agcgatttga ttcaggtaat
     3721 tttcaatatg tttacgcacg tcatttggta aaatgagaca atcatacccg aagtcataat
     3781 aataatagaa atgagtaatt aaaaatgtgc caggattcat caaatgaatg ttttcaaatg
     3841 taattagttc attgtttaat cgatagttat catcactttc gagtatgcca gtaagaatac
     3901 taaactcaaa tagggaagca ttagaaaaca cttgttcaat gatatgatta ttattgaaaa
     3961 atgctgtcat aatcaattta acaagatttt gcttgttttt atttgagaat cctttgatac
     4021 catactgtct gcaaattgct tttagctcat cattagtttt taacagtaag gcctgttcca
     4081 ttgtgacatc gtttaagcgc gtatgttgtc ttacacgaaa gatttcatca tttaaatgca
     4141 tatggtcttt tgtatctaat gtttcgaatt caccatatgc actatttagt acctctgaat
     4201 tgatataact taaaaataaa ttatttacaa agaacgtatc ttcgttaatt tgaataacaa
     4261 aacctagatg atttgttagc tttaaaatat taacctcatg gctatttata attgtgacag
     4321 gattaccctt agatataagt gtttctataa atttttgttc tgtcttattc atatcatgat
     4381 aaatcttatg taaattttca ggacgtaaag catgcatcgt gacttcaaat ccttctttgt
     4441 cgttcggtaa attaaaatat tcgcatgcat tttgtaactt tcctgggtag ataaaatgag
     4501 catcaagcat ttttacacac ctcattgtat tttttaagtt tatcataaca tgattttatg
     4561 ccaggataaa aaaataacag catcttaaca aatgtaagat actgtcagtg aaactgtgaa
     4621 aactttagtt tctgataata tagtcaaagg catttaatgc tgcatttgca ccagcgccca
     4681 ttgaaatgat aatttgtttg ttcttctgat ctgtgacatc gccagcagca aatattccag
     4741 gtacgttagt attattgtta cgatcaataa caatttcacc acgttcgttt aattcaacag
     4801 catcttttaa ccatgatgtg tttggaagta aaccaatttg aacaaagata ccatctaagt
     4861 taagtagatg ttcttcgccg gtgctcatgt cttcgtaacg tatacctgta acatggtctt
     4921 ctccgacaac ttcagtagtt ttggcatttg ttttgatatc aacatttgat aaagaacgta
     4981 aacgatcttg taacacgttg tctgctttta attcgctagc gaattcgaat aatgtaacat
     5041 gattaacgat accagcaagg tcaattgctg cttcaacccc agagttaccg ccaccgataa
     5101 ctgctacgtc tttattttca aatagaggtc cgtcacagtg cgggcagaat gcaacacctt
     5161 tattaatcaa ttgctcttca cctggaatgt ttagcttacg ccaacctgca ccagtagcaa
     5221 taatgactgt tttactttct aagacagcac cgttttctaa cgtaacttta attgcttcgt
     5281 cagtcttttc gatatctgta gcacgaatgc ctgtcattgc atcaatgtca tattgatcaa
     5341 tgtgcgctgc taagttagaa gaaaattcag aaccagttgt ttctttaaca gtaatgaagt
     5401 tctcaatacc agcagtatca ttaacttggc caccgatacg atcagcaact atactagtac
     5461 gtaaaccttt acgtgctgtg taaatcgctg cactaccact agcaggacca ccaccaacga
     5521 ttaagacatc ataaggttct ttattttcaa actcagatgc atctgccgta ctgcctagtt
     5581 tcgaaagaat atcttggatt gtcatacgac cattgccaaa ttcttcgcca tttaaaaaga
     5641 cagcagggac tgccatgatg ttttcagatt cttcacggaa cactgcacca tcaatcatag
     5701 aatgcgtgat gttagggttg atcacactca ttaagttaag tgcttgaacg acatcaggac
     5761 atttttgaca cgttaaacta atgaatgttt caaaatggaa tgaaccttct aagtttttaa
     5821 tttggtcaat gattgactgt ttttctttag gtgcacgacc actaacctgt aaaattgcta
     5881 aaacaagtga gttaaactcg tgacctaatg gaatacctgc aaatgttata cctgtttctt
     5941 caccaggacg attgactgag aaacttggtg tacgttttaa agacttttca gaaagtgaaa
     6001 gtctaggtga catatcagaa atttctgtta ataattcttt tagttcttta gatttatcat
     6061 ctgaaccaag gctggcaacg aattcaacgt tgccctccat tagttctaat agttgtttaa
     6121 gttgttgttt taaatcagca ttaagcatgg ttgtaatgcc tccttagatt ttacctacta
     6181 aatctaaacc aggttgcaat gttttagcgc cttcttccca tttagctggg catacttcgc
     6241 cagggttttt acgaacatat tgagctgctt tgattttgtg agctaatgta ctagcgtcac
     6301 gtccaattcc gtcagcgtta atttcagatg cttgtacaac accgtctggg tcgataatga
     6361 atgtaccacg ttgagctaag ccagtagctt catctaatac atcaaaatta cgagtgattg
     6421 tttgtgatgg gtcaccaatc atagtgtaag tgattttgct aattgcatct gaatggtcat
     6481 gccatgcttt gtgtacgaag tgagtatcag ttgatactga gaatacattt acgcctaatt
     6541 tttgtaattc ttcatattgg ttttgtaagt cttctaattc agttggacaa acgaatgaga
     6601 agtcagcagg atagaagcat actacgctcc aagaaccttt taaatcttct tgtgtaactt
     6661 ctttaaattg atcttttttt ggatcgaaag cttgcgctgt aaatggtaag atttctttgt
     6721 taattaatga cataaatatc ttcctcctaa gaatttaagt atgaattaga actatcaatt
     6781 gattgtgctt aattataata attctaatct cttagttagc attattacat tttgatccag
     6841 aatagtcaac tggataactt tgtaaagtga atgattactt ttaaaataaa gaaagataat
     6901 ataaagtgct ttgataatgg attttgtagt tgatgattta aaaggttgtg tctatattta
     6961 atatcttgat tttaatgtaa aaaatgtaaa aaaagaagat ttgtattctc aactaagtca
     7021 accttattga taatggtgtg agaatatttg ttcgagatgg atgaaggtaa tgcgtgagaa
     7081 actggatttt tcaagtacga gacaatattt ttaaaagttc aattattaac ttataagcaa
     7141 ataattgcta taaaaaagtt tggacgtgta caattgcaat atgaagattt ttaattaact
     7201 gtaaagtatc gaggagtggg taacgtgtca gatcatgtat ataatcttgt gaaaaagcat
     7261 cattctgtta gaaaatttaa gaataagcct ttaagtgaag acgttgttaa gaaattggta
     7321 gaagctggac aaagcgcttc gacgtcaagt ttcctgcaag catactcaat tattggtatc
     7381 gacgatgaga agattaagga aaatttacga gaagtttctg gacaacctta tgttgtagaa
     7441 aatggctatt tattcgtctt tgttattgat tattatcgtc atcatttagt tgatcaacat
     7501 gctgaaactg atatggaaaa tgcatatggt tcaacggaag gtttgctagt aggtgcaatc
     7561 gatgcagcat tagttgccga aaatattgcg gtaactgctg aagatatggg gtatggcatt
     7621 gtctttttag gatcattaag aaatgatgtt gaacgagttc gagaaatttt agacttacct
     7681 gactatgtct tcccgatatt tggtatggca gtaggggaac ccgcagatga cgaaaatggt
     7741 gcagccaagc cacgcttacc atttgaccat gtcttccatc ataataagta tcatgctgat
     7801 aaggaaacac agtatgcaca aatggcagat tacgaccaga caatcagcga gtactatgat
     7861 caacgtacaa acgggaatcg caaagaaaca tggtcgcagc aaattgagat gttcctagga
     7921 aacaaagcaa gattagatat gttagaacaa ttgcaaaaat caggcttaat acagcgatag
     7981 caagatacca aaataacccg ccccctctag cttaaaatga taagtatagc tagagggggc
     8041 gggtatttct tgcaatgaat tagtgtgaag ttaatgcagc attatcattt gattcgaaag
     8101 tatctttgtc ccaatgttta gttaacttgg cggtacctgt accagctagc attgaatcgt
     8161 tcacgtttaa tgctgttcta cccatgtcaa tcaatggttc aacggagatg agcacgccgg
     8221 ctaaagcgac tggcaagttt aacgttgaca acaccaatat ggatgcaaat gtagccccgc
     8281 caccgacgcc agcaacgccg aatgaactaa taatcacgac agcgattaac gttacaataa
     8341 attgtaaatc aatttctaca ttagcgacgg gtgcgaccat aattgcaagc atggcagggt
     8401 aaatgcctgc acaaccattt tgtccaatcg acaatccaaa tgtcgcagcg aaattggcaa
     8461 taccttctgg cacgcctaga cgtcttgttt gtgtttgtac attcagtggt aaggcgcccg
     8521 cacttgagcg tgatgtgaat gcaaagatta atacttccaa agtcttttta atatagcgaa
     8581 tagggctaat gcctaacagg cttaaaataa ttaagtgaat gatatacatc gtaattaatg
     8641 cagcgtacga tgcgattaag aattttccta aagtccaaat ggcgccaaag tcacttgtcg
     8701 ataacgtgtt ggccataatt gctaatacac cgtatggcgt taaacgtaag acgaacgtca
     8761 caatcgccat tactagtgaa tagatagcgt caatcgcacg cttaagcaat tcaccatgat
     8821 caggttgttt gcgtgctacg cgtaaataag caaatcctat aaacgaagca aatatcacga
     8881 cagcaatcgt ggaagttgca cgttgtccag tgaaatctaa gaatggattt ttaggcaata
     8941 attccaaaat ttgttgtggt aacgtatgtg ctgttaaatc tttcgcttgt ttagcaattt
     9001 cgcttccacg tgcttgttca gcgttaccaa gattaattgt tgatgcgtct aaaccaaaca
     9061 ccaaggcata cacaacacca acaattgcag caatggtgac agtgccaatt aaaaagataa
     9121 aaatgagact accaatttta gcaaactttt ctccgatttg aattttagtg aatgcagcga
     9181 caatagaaat gaaaattaaa ggcatcacaa tcatttgcaa caatgcaacg taaccttgtc
     9241 cgacaatgtt gaaccagtca cttgttgatg taataacatt cgaatgtgtt ccatagacaa
     9301 gatgtaataa cacgccaaat acaatgccga tacctaaagc tgtaaacaca cgtttcgcaa
     9361 aagatatatg tttgcgagcc atcatgtgca atattacgat gaaaatcacc aatacaataa
     9421 tattaatcag tgtaagaaaa gcattcatga acgtcactcc tcaaattttt gaatataatt
     9481 ccgactagta tgctaagaat aatattaatt ttaaaatatt tcaagagggc atttgcaatt
     9541 aaatgtaaac atgatgaaat tcgaccgttg gcataaggga atttgtaaac aaatcgtttt
     9601 gaatttagta gagaaaggtg aaaagggtta atagtgttat gacctagtcg attaaagttg
     9661 atatttgaaa atgaagcagg gcattaaatg taataaatta aataagttgt cattacagcg
     9721 ttaataatag aaatgatttt aacaggaaaa aagtgatgaa tatttggaaa agatatatat
     9781 cgtgcactgt cataagagat aagatatgag aaaacaaatg catccttgaa tcgagatgtg
     9841 gctatgacgg tttgataaaa attgctaacc tcatatttat tgaataaaaa gataataaat
     9901 gatcaacctt gaaaagacgc ttcgaacaaa cacgttgtaa aaaattctgt cgaatcaaaa
     9961 ttagcatctt ttgagtgttc gtgtgtgcag gtgggagttc aaaagctatc gtaaaaaaat
    10021 atttttgttt gagcaaaaag gcgtttgtag taaatgtgaa tatgagtgag cccttgtgcg
    10081 attcgtcatt tactaacaca gccaaacatt taaattgaat gggtttatta agatgaatct
    10141 atttaggaat acacaggagc catcccattt aaaagtaaca tactataaaa atttattcaa
    10201 aattcaatat taataataat gaataattaa aaaacctgaa acaaattagt ttgatatgct
    10261 ttcttcaaag tagatattga atggagtata tcagcttgtt tcaggtttta tgaattgagt
    10321 attaaatttg aaagtaaatt acagttctat aggtttataa
//
LOCUS       DAHHOJ010000093        10118 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.093, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000093 DAHHOJ010000000
VERSION     DAHHOJ010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10118)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10118)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10118
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.093"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            24..1394
                     /locus_tag="NBH32_002052"
     CDS             24..1394
                     /locus_tag="NBH32_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001670170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCD1515633.1"
                     /translation="MDSFSRKEIVIGRLKFITMSLIGILLFLVPIPVEQDGQKQTTLP
                     VAFLAGVLKDVLGGVMPFLIVTIITLSGIITLICSTILKDKLKPDGLMNNAFNVRIGW
                     LILRLLAVVFAWMTFLRIGSKVIYSDETGGLLFSSLLPTLVAVFLFAALFLPLLMEYG
                     LLEMLGPIFRPVMRPLFTLPGRSTVDNLASFIGDGTVGVLITSRQYGEGYYSRREATV
                     ISTTFSVVSITFAIVVAETVHMQNQFFAFYLSVIVSCLVAAVIMPRIWPLNKIPDEYA
                     KEVPESARTEALPEGKTALRHGFDTATEVGIKAPGVVDFFKSGLKTVVDMWFVILPVV
                     MSIGTIATIIANYTPFFVILGKPFVPFLELMQIPEAAQASQTIIIGFADMFLPSILIE
                     GVQNDITRFVIGALSISQLIYLSEVGGVILGSKIPVSIGKLFMIFLIRTIITLPIISL
                     MAHLLL"
     gene            complement(1526..1786)
                     /locus_tag="NBH32_002053"
     CDS             complement(1526..1786)
                     /locus_tag="NBH32_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515634.1"
                     /translation="MAMTVKKDNNEVRIQWRVADIKIPTSEIKNITQDQDIHAVPKLD
                     SKDVSRIGSTFGKTNRVIIDTEDHEYIIYTQNDQKVYNELTK"
     gene            1963..2478
                     /locus_tag="NBH32_002054"
     CDS             1963..2478
                     /locus_tag="NBH32_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCD1515635.1"
                     /translation="MTGKTHASCGMLVGALTTQYFQTDIFSSVTVIILATLASLLPDI
                     CHTQSKIGRRFKVISFFVRLIFGHRTFTHSILFIAIIAFLLQIIQTPNYYMAAIIIGL
                     VSHVILDMITPKGVKLFYPLPFNVKTPIQFKTGGLVDLSLATALMVGTVYVLFQPFIN
                     DIISNWNTKFL"
     gene            2522..3709
                     /locus_tag="NBH32_002055"
     CDS             2522..3709
                     /locus_tag="NBH32_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDPGP type 1 family protein"
                     /protein_id="HCD1515636.1"
                     /translation="MLDKNQLAKYKQDHLCEYEKIMSNNEKEALEEKVASLDLDFIAK
                     LYNDLYINKKTIDDVSAVSEVKYDIKSQMSDDEIKRLEGQGLQAIKEGQFAVLLMAGG
                     QGTRLGYKGPKGSFEIEGVSLFELQANQLKTLNHQSGHTIQWYIMTSDINHEETLAYF
                     EAHSYFGYDQEAIHFFKQDNIVALSEEGKLILNQQGRIMETPNGNGGVFKSLDKAGYL
                     EEMSNNGVKYIFLNNIDNVLVKVLDPLFAGFTVEHDYDITSKTIQPKPGESVGRLVNV
                     DCKDTVLEYSELDPEVANQFNNANIGIHAFKLGFILNAVNRELPYHLAIKNLKQLDEN
                     FGVIEQPTLKFELFYFDIFTYGTSFVTLQVPREEEFSPLKNTEGKDSVATATEDLRRM
                     GLI"
     gene            3728..4411
                     /locus_tag="NBH32_002056"
     CDS             3728..4411
                     /locus_tag="NBH32_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCD1515637.1"
                     /translation="MSKSSEKSNDTIAETFKDIIPLSFGEEIGNAASHGLAALLTLLV
                     LPYAAVHSYIANGTLASVSMSIYVISIFMMFISSTIYHSMQNETPHKYILRIIDHSMI
                     YVAISGTYTPILLTVVGGWIGWTVFILLWGTTLWGILYKSIAVKVNQKLSLIVYLVMG
                     WVGIILLPIIFIRTSWIFILFIFLGGVSYTIGAWFYAQKNRPYFHMIWHIFIVIASLL
                     HLIAILYFM"
     gene            4691..6034
                     /locus_tag="NBH32_002057"
     CDS             4691..6034
                     /locus_tag="NBH32_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515638.1"
                     /translation="MRLKSIVTVIALILIMFMAAIESSIISLALPTIKQDLNAGNLIS
                     LIFTAYFIALVIANPIVGELLTRFKIIYIAIAGLTLFTVGSLMSGLSTHFSMLIISRV
                     IQGFGSGVMMSLSQIVPKLAFEIPLRYKIMGIVGSVWGISSIIGPLLGGGILEFATWH
                     WLFFINIPIAIIAIILVVWTFHFSEEETVAKSKFDTKGITLFYIFIGLIMFALLNQQH
                     LYLNIIGFVLAILIALRLFNVEKKVSSPFLPVAEFNRMITLVFITDLLTAVCLMGFNL
                     YIPVYLQEQLGLSPLQSGLVIFPLSVAWITLNFNLHHIEAKLSRKVIYLSSFTLLLLS
                     SIIIAFGIKLPILIACVLILSGISFGYIYTKDSVIVQEETSPIQMKKMMSFYGLTKNL
                     GASIGSTIMGYLYALKSGLFGANLHNILGVVSLISVGLIVVWLIFYREAASQTNE"
     gene            6156..6629
                     /gene="sepA"
                     /locus_tag="NBH32_002058"
     CDS             6156..6629
                     /gene="sepA"
                     /locus_tag="NBH32_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter SepA"
                     /protein_id="HCD1515639.1"
                     /translation="MVMNYLKHKFYNLLTTIIVLFIFVLSGAIFLTFLGFGLYGLSRI
                     LIYFRLGDFTYNRSMYDNLLYYGSYIIFGYFIIFAVEHLMDYFRKMLPENAYFRGATF
                     HLISYMVATTLFYFIIHLNYVYINIDFWVIMVIIGFLYVCKLQFYPESKNLNNRK"
     gene            6818..8260
                     /gene="lmrS"
                     /locus_tag="NBH32_002059"
     CDS             6818..8260
                     /gene="lmrS"
                     /locus_tag="NBH32_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter LmrS"
                     /protein_id="HCD1515640.1"
                     /translation="MANVELTTRRRNFIVAVMLISAFVAILNQTLLNTALPSIMRELN
                     INESTSQWLVTGFMLVNGVMIPLTAYLMDRIKTRPLYLAAMGTFLLGSIVAALAPNFG
                     VLMLARVIQAMGAGVLMPLMQFTLFTLFSKEHRGFAMGLAGLVIQFAPAIGPTVTGLI
                     IDQASWRVPFIIIVGIALVAFIFGLISISSYNEVKYTKLDKRSVMYSTIGFGLMLYAF
                     SSAGDLGFTSPIVIGALIISMVIIYLFIRRQFNISNALLNLRVFKNRTFALCTISSMI
                     IMMSMVGPALLIPLYVQNSLSLSALLSGLVIMPGAIINGIMSVFTGKFYDKYGPRPLI
                     YTGFTILTITTIMLCFLHTDTSYTYLIVVYAIRMFSVSLLMMPINTTGINSLRNEEIS
                     HGTAIMNFGRVMAGSLGTALMVTLMSFGAKIFSSTSPSHLTATEIKQQSIAIGVDISF
                     AFVAVLVMAAYVIALFIREPKEIESNRRKF"
     gene            8408..9472
                     /locus_tag="NBH32_002060"
     CDS             8408..9472
                     /locus_tag="NBH32_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017804887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-loop NTPase"
                     /protein_id="HCD1515641.1"
                     /translation="MLTVDQVKELVGEIKDPIIDVPLKETEGIVEVSIKEEKEHVSVK
                     LAMAQLGGAPQLDLQMAVVNVLKENGAKTVGIRFETLPEEKVNQFKPKEENKPKTIEG
                     LLSQNNPVEFIAIASGKGGVGKSTVAVNLAVALAREGKKVGLVDADIYGFSVPDMMGI
                     DEKPGIKGKEVIPVERHGVKVISMAFFVEENAPVIWRGPMLGKMLTNFFTEVKWGDIE
                     YLILDLPPGTGDVALDVHTMLPSSKEIIVTTPHPTAAFVAARAGAMAKHTDHSILGVI
                     ENMSYFESKETGNKEYVFGKGGGTKLADELNTQLLGELPLEQPSWNPKDFAPSIYQSD
                     DRLGKIYSSIAQKVIAATNK"
     gene            9561..9746
                     /locus_tag="NBH32_002061"
     CDS             9561..9746
                     /locus_tag="NBH32_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515642.1"
                     /translation="MLSNKRVEKEAGCEHKKFLKKLLTLNSKVDNMIKSSKTTQLFLK
                     KNFKSVKLTIEITIKTI"
     gene            9930..>10118
                     /locus_tag="NBH32_002062"
     rRNA            9930..>10118
                     /locus_tag="NBH32_002062"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         3280 a         1357 c         1915 g         3566 t
ORIGIN      
        1 acatagagaa atggattgat tttatggata gcttttcaag gaaagagata gttattggga
       61 gacttaagtt tattacgatg tctttaattg gtatcttatt atttttagta ccaattccag
      121 tcgaacaaga tggacagaag caaacaacgt tgcccgtggc atttttagca ggcgtgctta
      181 aagatgtatt aggtggcgta atgccatttt taattgtgac gataattact ttgtcgggta
      241 tcataacact gatttgttca actattttaa aagataaatt gaaacctgac gggctaatga
      301 ataatgcttt taacgttaga attggttggt taattttgcg cctacttgca gttgtttttg
      361 catggatgac atttttacga attggttcaa aggttattta ctcagatgaa acgggtggat
      421 tattgttctc aagtttattg ccaacattag tggcagtatt cttatttgca gctctatttt
      481 taccgctatt aatggaatac ggtttgttag agatgttggg tcctattttt agaccggtta
      541 tgagaccctt atttacattg ccaggacgtt cgacggtaga taatttagcc tcatttattg
      601 gagacggaac tgttggagtc ttaattacaa gtagacaata tggtgaagga tattattctc
      661 gtcgtgaggc aacagttatt tcaacgacat ttagtgtagt ttctatcacc ttcgcaattg
      721 tagttgctga aacagtgcat atgcaaaatc aattttttgc tttctattta tcagtgatag
      781 tatcctgcct cgttgcagca gtcattatgc caagaatttg gcctttaaat aaaattcctg
      841 atgagtatgc taaggaggta cctgaaagtg cgcgtactga ggcattgcca gaaggaaaga
      901 cggcattgag acatggcttt gatactgcaa cagaagtagg tattaaagca ccgggcgttg
      961 ttgacttttt taagtcagga ttaaaaacag tcgttgatat gtggtttgtt attttgcctg
     1021 tagtgatgag tattggtact attgctacca ttattgcgaa ctacacgccc ttttttgtca
     1081 tattgggtaa accttttgtt ccatttttag agttaatgca aatacctgaa gcggcacagg
     1141 cttctcaaac gattattatc ggtttcgccg atatgttttt accttctata ttaattgaag
     1201 gtgtacaaaa tgatataact cgttttgtta ttggggcact tagtatatcg cagttaatat
     1261 acttgtctga agtaggtggc gtcatacttg gttcgaagat tcctgtaagc atcggcaaat
     1321 tgtttatgat tttcttgatt agaacaatca ttacactacc aattatttct ttgatggcac
     1381 atcttttgtt ataaatatat gtggaaaagg acaactgtta aaattatgga aacagttgtc
     1441 cttttgtatt tgtttatgct gggtgttgaa ccaattaaaa aaatcattca tgaagccctc
     1501 accatgaatg atttttttat acaatttatt tagttaattc attgtaaact ttttgatcgt
     1561 tttgagtata aataatgtat tcgtggtctt cagtatcgat aataacgcga ttcgttttac
     1621 caaacgttga gccgattcta gatacatctt tgctgtctaa tttaggaact gcatgaatat
     1681 cttggtcttg tgtaatattt ttaatttcac ttgtaggaat tttgatatca gcaactctcc
     1741 attgaatacg cacttcatta ttatctttct ttactgtcat tgccattatt caaacacatc
     1801 ctttaaatat tttgtaaata catcataaac gattttgtaa tcgttttcaa gagttatgtg
     1861 atactttttt aaattgtgtc atactaatga tttttaaccc gaaaatatgt tcgtgttttt
     1921 gttagggata tggtaaaatg tgagtattaa aggagaacaa atatgacagg taaaacacat
     1981 gcttcatgcg gcatgctcgt tggagcgcta acaacacaat attttcaaac ggatattttt
     2041 tcgtcggtta cggttatcat tctagcaact ctagctagct tattacccga tatatgtcac
     2101 actcaaagta agattggcag aaggtttaag gtaattagtt tttttgtgag attgatcttt
     2161 ggacatcgaa cctttacgca ttcaatatta tttatcgcaa tcatagcatt tttattgcaa
     2221 attattcaga cgcctaatta ctatatggca gcaattataa ttggattagt gtcacatgtt
     2281 atactagata tgataacacc aaaaggcgtg aaattatttt atccgttacc gtttaatgtg
     2341 aagacaccga ttcaatttaa aacaggtgga cttgtagact tatctttagc tactgcgtta
     2401 atggtcggaa cagtttatgt tttatttcag cctttcatca atgatataat aagcaactgg
     2461 aatactaagt ttttatagaa aataaattat taaaggttac aaactcagca ggaggcataa
     2521 aatgctagat aaaaatcaat tagctaaata taaacaagat catttgtgtg aatatgaaaa
     2581 aataatgagt aacaatgaaa aagaagcact tgaagagaag gtagcgtcat tagatttaga
     2641 ttttattgcc aaattatata acgatttata catcaataag aaaacaatag atgatgtatc
     2701 tgcagtttct gaagtgaaat atgatattaa atcacaaatg agtgatgatg agattaagcg
     2761 tttagaggga caaggacttc aagctattaa agaaggacaa tttgcagtac ttttgatggc
     2821 aggtggacaa ggaacaagac ttggatataa aggacctaaa ggttcgtttg aaattgaagg
     2881 tgtaagttta tttgaacttc aagctaacca attaaaaaca ttgaatcatc aatctggaca
     2941 tacgattcaa tggtatatta tgacaagtga tatcaatcat gaagaaactt tagcttattt
     3001 cgaagcacat agttattttg gatatgatca agaagcaatc catttcttta aacaagataa
     3061 tattgtggcg cttagtgaag aagggaagtt aattttaaat caacagggtc gtattatgga
     3121 aacgccgaat ggtaatggtg gcgtgtttaa atctttagac aaggcaggat acttagaaga
     3181 aatgtctaat aatggcgtta agtatatttt cttgaataat atcgacaatg ttttagtaaa
     3241 agtgttggat ccactatttg caggatttac ggtagaacat gattacgata ttacttctaa
     3301 aacgattcaa ccaaaaccag gagaaagtgt tggacgttta gttaatgtcg attgtaaaga
     3361 tacagtactg gagtattcag aattagatcc agaagttgca aatcaattta acaatgctaa
     3421 tatcggtatt catgcattta aacttggttt tattttaaac gctgtaaatc gtgaattacc
     3481 ttatcattta gctattaaga atttgaaaca attagatgag aactttggtg ttattgaaca
     3541 accaacttta aaattcgaat tgttctattt tgacatattt acttatggaa ctagttttgt
     3601 cacgttacaa gttcctagag aagaagaatt ttcacctctt aaaaatacag aaggtaaaga
     3661 tagtgttgca actgcaacag aagatttacg tagaatgggt ttaatttaat gataggcggt
     3721 gactatgatg tccaaatcta gtgaaaaaag taatgacaca attgcagaaa catttaagga
     3781 tatcattcca ttgtcatttg gagaagaaat aggcaatgct gcatctcatg ggttagcagc
     3841 gctgttaaca ctattagtct taccttatgc ggctgtacat agttatattg caaatggcac
     3901 acttgcatca gttagtatgt caatttatgt tatttctatc tttatgatgt ttatttcatc
     3961 tactatttat cattctatgc aaaatgaaac gcctcataaa tatattttaa ggattattga
     4021 tcatagtatg atttatgtag caatatcagg tacatatacg cctattttat taacagttgt
     4081 aggtggttgg attggatgga cagtgtttat cctattatgg ggcacaacgc tttggggaat
     4141 tttatataaa tccattgctg tcaaagtaaa ccaaaagtta agcttaattg tctatttggt
     4201 aatgggttgg gtaggtatta tcttgctacc aataatattt attcgtacat cttggatatt
     4261 tattttattt atttttctag gcggtgtgtc atatacgatt ggtgcatggt tctatgcaca
     4321 aaaaaacaga ccatattttc atatgatatg gcatattttt atcgttatag catcattatt
     4381 gcatttaatt gcaatcttat attttatgta gtcgtttaga aaaggtgggg ggaactccgc
     4441 tttttttatt acttaattca ttctaataga ggattgtttt gcttttttta aatcggtatg
     4501 atttttaaag ctgggagtgc acttaatttg tattaatcag acatttgatg gatacgttta
     4561 tttgaatttt ttagtaggtg caacaaatag attttttaaa aaactaattt gaatttaaaa
     4621 gagggcaaga taggaaatag taaaattaga aattacaaag cactatcatt attataaaag
     4681 gaggtatagc atgcgtttaa aatcaattgt aactgtaata gctttaatat taatcatgtt
     4741 tatggctgca atcgaatcat caattatttc tttagcgtta ccaacaatta agcaagattt
     4801 aaatgcaggg aatttaattt cattaatatt tacggcttac tttattgcat tagtgattgc
     4861 gaatccaata gtaggtgaat tattaactcg atttaaaatt atttatattg ctatcgcagg
     4921 tcttacacta ttcacggttg gtagtttaat gtcaggttta agcacacatt ttagtatgtt
     4981 gattatatca cgtgtaattc aagggtttgg atctggtgtc atgatgtcct tgtcgcaaat
     5041 tgtgcctaaa ctagcgtttg aaattccatt acgctataaa ataatgggta tcgttggtag
     5101 cgtttggggg atttcaagta tcattggtcc gttactagga ggcggtatac ttgaatttgc
     5161 aacttggcat tggttatttt ttattaatat tccgatagca attattgcaa taatattggt
     5221 tgtctggaca tttcattttt cagaagaaga aactgtggct aaatcaaaat tcgatactaa
     5281 aggtatcacc ttgttttata ttttcattgg tttaattatg tttgcattgt taaaccaaca
     5341 gcatctatat ttaaatatca taggttttgt tttagcaata ttaattgcac ttcgattatt
     5401 taatgttgag aaaaaagttt catccccatt cttaccagta gcagaattta atcgaatgat
     5461 tactttggtc tttattacag atcttcttac agctgtttgt ttaatgggct ttaatttata
     5521 tattcctgtg tatcttcaag aacaactggg attatcgcca ttgcaaagtg gattagttat
     5581 ttttccactt tctgtagcgt ggattacatt gaattttaat ttgcaccata ttgaagcgaa
     5641 gttatcaaga aaagttatct atttgtcgtc ttttacatta ttattattaa gtagtataat
     5701 cattgcgttt ggtataaaat taccaatcct tatagcatgt gtcttaattt taagtggaat
     5761 aagctttgga tatatttata caaaagatag tgtaattgtc caagaggaaa caagtccaat
     5821 acaaatgaag aaaatgatgt cgttttatgg attaacaaaa aaccttggcg catcaatagg
     5881 ttcgacgatt atgggatacc tttatgcatt aaaatcagga ttatttggtg caaacttgca
     5941 taatatttta ggcgttgtat ccttgataag tgtagggctc atcgttgtat ggctcatatt
     6001 ttatagagaa gcggcgtcac aaacaaatga atagaattat cattaaaatt gatgtagtgt
     6061 aaataaagta agaaataatc attttactag gtttgaagtg taatcaacta tcttaaaatg
     6121 aaaagcaata taaattattt atggtaagga ggacaatagt tatgaattat cttaaacata
     6181 agttttataa tttattaaca acaattattg ttctctttat ctttgtgctt tctggtgcga
     6241 tatttttaac gtttttagga ttcggactat atggattaag tagaattctt atttatttta
     6301 ggttaggtga cttcacatat aacagaagta tgtacgacaa cctattgtat tatggcagtt
     6361 atataatctt tgggtacttc atcatatttg cagtcgagca tttaatggat tattttagaa
     6421 aaatgttacc tgaaaatgct tattttagag gtgcaacttt tcatttaatc tcttatatgg
     6481 ttgcaacaac gttattttat tttattattc atctgaacta tgtatatatc aatattgatt
     6541 tttgggtcat catggttatt ataggctttt tatatgtttg taagttgcag ttttaccctg
     6601 aaagtaaaaa tctaaacaat agaaaataga gttttgaagt tgggggcaat gatgtgttcc
     6661 aacttctttt tattcatata gtagaaaatg agttgttttg cttcaattaa tcacggttaa
     6721 taataaccat tgatataaat agttggtact gtaatctgaa atatatttta agtatatatg
     6781 ctaataaacg taaatttgtt acaaaggagg aattgtggtg gctaatgttg aattaacaac
     6841 gcgaagacga aattttatag tagctgtgat gttaatcagt gcttttgtag ctattttaaa
     6901 tcaaacatta ttaaatacag cgttacctag tattatgaga gagttaaata tcaatgaaag
     6961 tacttcacaa tggttagtta ctggttttat gcttgttaat ggcgtcatga tacctctaac
     7021 tgcatactta atggacagga ttaaaactag acctttatac ttagcagcga tgggaacatt
     7081 tttattaggt tctattgttg cagccttagc tccgaatttt ggagttttaa tgttagcacg
     7141 tgtaattcaa gcgatgggtg caggggtact tatgccttta atgcaattca cattgtttac
     7201 gttattcagt aaagaacata gaggatttgc aatgggatta gctggtttag ttattcaatt
     7261 tgcaccagca attgggccta ctgttactgg attaatcatt gatcaagcaa gttggcgtgt
     7321 gccttttatt ataattgtag gaattgcttt agtcgcattt atcttcggtt taatttcaat
     7381 ctctagttac aatgaagtga aatatacgaa attagataag cgctctgtaa tgtattcgac
     7441 gattggattt gggttaatgt tatatgcatt cagtagtgca ggcgacttag gatttactag
     7501 cccgatagtt ataggtgcat tgataataag tatggttatt atctatttat ttatacgtag
     7561 acaatttaat attagtaatg cacttttaaa tttaagggtt tttaagaata gaacatttgc
     7621 attatgtacg attagttcaa tgattataat gatgtcaatg gttggacctg cactgcttat
     7681 accgctatat gttcaaaata gtttgtcttt atctgccttg ttatcaggac ttgttatcat
     7741 gccaggtgca atcataaatg gtattatgtc ggtttttaca ggtaaatttt atgataagta
     7801 tggtcctaga ccactgattt atactggttt tacgatttta acaattacta ctattatgtt
     7861 gtgtttcttg catacagaca catcttatac gtatttaata gtcgtatatg caattagaat
     7921 gttttcagtt tctttactca tgatgccgat aaatactaca ggaattaatt ctttgagaaa
     7981 tgaagaaatc tcacatggca cggctattat gaactttggt cgtgtaatgg ctggttcact
     8041 aggcacagct ttaatggtta ctttgatgag ttttggcgca aaaatatttt catctacatc
     8101 gccatcgcat ttaactgcaa ctgaaattaa acagcaatca attgctatag gggtggatat
     8161 ctcatttgct tttgtagccg tgcttgttat ggcagcttat gtgatagcac tttttataag
     8221 agaacctaaa gaaatagaaa gtaatagaag gaaattttaa aataattata gtagttggtc
     8281 tattgaaaat aataggctaa cttctttttt tatttaataa aaagttttat acttttagtg
     8341 atagactaag caaaaattgt tatttgctat gatgtagatg tcttaaaatg attaaggggg
     8401 atttgctttg ttaacggtag atcaagtgaa agaattggta ggagaaatta aagaccctat
     8461 tatagatgtg cctttaaaag aaacagaagg tattgttgaa gtttctatta aggaagaaaa
     8521 agaacatgtg agtgttaaat tagcaatggc acaattaggt ggtgcaccgc aattagattt
     8581 acaaatggct gttgttaacg tattaaaaga aaacggtgcg aaaacggtcg gtatacgatt
     8641 tgaaacattg ccggaagaaa aagtaaatca atttaaacca aaagaagaaa ataaacctaa
     8701 aacgatagaa ggcttattat ctcaaaataa tccagtcgaa tttattgcta ttgcttcagg
     8761 taaaggtggc gtcggtaaat ccactgttgc agtaaattta gccgttgcct tagcccgtga
     8821 agggaaaaaa gtcggattag tagatgccga tatatatgga tttagtgtac cagatatgat
     8881 gggtattgat gaaaaacctg gaattaaagg gaaggaagta attccagttg aacgtcatgg
     8941 cgttaaagtt atatcaatgg ccttttttgt ggaagaaaat gcgccagtta tatggagagg
     9001 gccaatgtta ggtaaaatgt tgacgaattt ctttacagaa gttaaatggg gagacattga
     9061 atatttaata ctcgatcttc cacctggaac aggagatgta gctttagatg ttcatacgat
     9121 gttaccttca agtaaggaaa ttattgtaac gacacctcat cctacagcag catttgttgc
     9181 agctcgcgca ggtgcgatgg caaaacatac ggatcattct attcttggcg taattgaaaa
     9241 catgagttat tttgaaagta aagagacggg taataaagaa tatgtctttg gcaaaggtgg
     9301 cggtactaag ttagctgatg aacttaatac tcaattactt ggggaattac ctttagagca
     9361 accatcttgg aatccaaaag attttgcacc gtcaatatat caatcagatg atcgtctagg
     9421 taaaatttat agctcgattg cacaaaaagt tatagctgct acaaataaat aggtataata
     9481 tatgctaaac gtacaaaatt acttttattt tgtacgtttt tatgttgatt tgttttggaa
     9541 tgctggtaaa ataagttctt gtgttaagca ataaacgcgt tgaaaaagaa gcgggttgtg
     9601 aacacaaaaa gtttttaaaa aagttattga ctttaaattc caaagttgat aatatgataa
     9661 agtcgtcaaa aacgacgcag ttgtttttaa agaagaattt caaaagtgta aaacttacca
     9721 ttgaaataac tataaaaaca atatagaata aataaagtaa tcgttcaaaa agttattgac
     9781 ttcaagaaac aaatgaagta gaataaaaga gattgaacat taatgaacat tgaaaactga
     9841 atgacaatat gtcaacgtta attccaaaaa acgtaactat aagttacaaa cattatttag
     9901 tatttatgag ctaatcaaac atcataattt ttatggagag tttgatcctg gctcaggatg
     9961 aacgctggcg gcgtgcctaa tacatgcaag tcgagcgaac ggatgagaag cttgcttctc
    10021 tgatgttagc ggcggacggg tgagtaacac gtggataacc tacctataag actgggataa
    10081 cttcgggaaa ccggagctaa taccggataa tattttga
//
LOCUS       DAHHOJ010000094        10055 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.094, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000094 DAHHOJ010000000
VERSION     DAHHOJ010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.094"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            226..861
                     /locus_tag="NBH32_002063"
     CDS             226..861
                     /locus_tag="NBH32_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HCD1515643.1"
                     /translation="MSDQVKIPRATLKRLPLYYRFVSSLKSKGIDRVNSKAISDALQI
                     DSATIRRDFSYFGELGKKGYGYNIDSLLDFFKSELSESDMIKIAIVGVGNLGKALLTY
                     NFSIHDDMTITEAFDVKEDVIGQKIGNVIVKDNDELITTLKKEEIDVVILTTPERVAQ
                     KVADELVQAGVKGILNFTPGRINTPSDVQVHQIDLGIELQSLLFFMKNYSE"
     gene            1162..2238
                     /locus_tag="NBH32_002064"
     CDS             1162..2238
                     /locus_tag="NBH32_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HCD1515644.1"
                     /translation="MMWMIFSGLIVGGLLGFVMQRTRFCLTGGFRDMYVQKNNKMFYA
                     LLIAITIQSIGLLILTATDILQIPAHSFPILGTIIGSFIFGIGIVLAGGCATGTWYRA
                     GEGLIGSWIALVLYAVTAAITKTGILKPVMDKINQPTNVNSDMSQTTGIPFWGLVVIL
                     TIITIFLVVRTLNNKKVRVAVPKLKQRYTGIRYYLFEKRYHPFIAAIAIGLIALLAWP
                     MSASTGRNDGLGITTPSANLVHFLITGETKFIDWGVFLVLGIFIGSYIAARGSREFKW
                     RLPDKITIRNSAIGGICMGFGASVAGGCSIGNGLVETATMTWQGWIALASMIVGVWTM
                     SHFIFVRPMKKYNNNLQRSNSKRK"
     gene            2303..2527
                     /locus_tag="NBH32_002065"
     CDS             2303..2527
                     /locus_tag="NBH32_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCD1515645.1"
                     /translation="MIHELGTVGMVCPFPLIEAQKKMATLQSGDELKIDFDCTQATEA
                     IPNWAAENGYPVTNYEQIDNASWTITIQKV"
     gene            2735..3985
                     /locus_tag="NBH32_002066"
     CDS             2735..3985
                     /locus_tag="NBH32_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HCD1515646.1"
                     /translation="MNLNDTIFMFLCTLLVWLMTPGLSLFYGGLVQSKNALNTVMQSM
                     AAIVLVTFVWITVGFTISFGEGNLWFGNWEYTFLNHVGFATQEDISPHIPFALFMLFQ
                     MMFCTIAISILSGSIAEKMKFIPYLLFVVIWTALVYSPVAHWVWGGGWINKLGVLDFA
                     GGTVVHITSGVSGLVLAIMIGKGNKHSESTPHNLIITLIGGIFVWIGWYGFNVGSAFT
                     FDQIAMLAFTNTVISASAGAIGWLILEYIFKKTTSLLGLLLGALAGLVVITPAAGYVT
                     YLSATIMALIGGICCYIVINYIKVKLKYHDALDAFGIHGVGGIIGAVLTAVFQSKKAN
                     PDIENGFIYTGDIHIILVQILCVTAVVIFSIVMTFIIAKVIKLITPLSVTEQETNIGL
                     DKIVHGEHAYFEGELNRFNKHIRY"
     gene            4168..5118
                     /locus_tag="NBH32_002067"
     CDS             4168..5118
                     /locus_tag="NBH32_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCD1515647.1"
                     /translation="MKNISDIAKLAGVSKSTVSRFLNNGSVSKKTSEKLTKIIAEHDY
                     QPNQFAQSLRARQTHLIGAIIPRMNSYAVDETIKGLAKQCQKYESQLILNYTGLNIEA
                     EIQALETLARSKVDGIVLMATDITERHIEVINKMNVPIVIVGQQHEQLHSIVHDDYKA
                     GQIIGEWIGQQGYQQIEVFSVSEKDIAVGIHRKRGLLDQLAKYQIKPNIHETNFTYVE
                     AQKDVANVLENVEQVDAVVGATDTIALAAYKYYSDKKEVMKPHQIYGFGGDPMTQLVS
                     PSIKTIHYNYFEAGQCAMEEIQQMLKKQDMPYSVTVDVNI"
     gene            5267..6751
                     /locus_tag="NBH32_002068"
     CDS             5267..6751
                     /locus_tag="NBH32_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HCD1515648.1"
                     /translation="MTEWTREERYQRIEDVDTEYFKTLKQQVDQSKFRQQFHIQPETG
                     LLNDPNGLIFYKGKYYVSHQWFPLGAVHGLKYWYNYTSDDLINFKAEGPILNPDTKYD
                     SHGVYSGSAFEFNGHLYYMYTGNHRDNHWQRHASQMIARLKEDGSVEKFPKPVISQQP
                     EGYTSHFRDPKVFKYGEKYYAIIGAQNNDQQGRLLLYNTEDIINWHYLGEINTELDDF
                     GYMWECPDYFNVDNQDVILICPQGIEPKGDQFKNIYQSGYILGKFDIEKLTYEHENFV
                     ELDNGFDFYAPQTFLDEKGRRVLIGWMGLPEIEYPTDNEGWAHCLTIPRVLNVENGQL
                     KQRPYPALEKLRHNKETALGYANKFTRKLHPYEGKQYELIIDILDNDATEVYFELRTS
                     KTSSTLIAYNKRENKIILDRSDSGLLPTNVEGTTRSTILETPLKQLQIFVDTSSIEIF
                     CNDGERVLTSRIFPTEDALGIKTSTESGQVYLQFTKYDLKDEHK"
     gene            6748..7707
                     /locus_tag="NBH32_002069"
     CDS             6748..7707
                     /locus_tag="NBH32_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCD1515649.1"
                     /translation="MRRLFSIGEALIDFIPNITNANLKDVQTFTKQIGGAPCNVACTV
                     QKLGQQAYMITQLGNDAFGDSIIETISSIGVDVSKVYRTNEANTALAFVSLTEAGERD
                     FSFYRKPSADMLFEPSFVNDIDVNENDVVHFCSVDLIDSPMREAHYQLITKTLNANGT
                     VVFDPNVRLPLWDNAEDLRQTIHTFLPLAHIVKVSDEELEFITGIHDENKAIQSLFTG
                     NVTVVIYTKGADGAAVYLKNGINHYHSGYKVKPVDTTGAGDAFIGAVISRILATDVLN
                     LTQLFENEGEEILAFSNRVAAIVTTKYGAINSLPTLQEVEQAF"
     gene            complement(7896..8003)
                     /locus_tag="NBH32_002070"
     CDS             complement(7896..8003)
                     /locus_tag="NBH32_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515650.1"
                     /translation="MHCIAGFLILIAKLNMTRELKNDVTSLCIPIPITT"
     gene            complement(8075..8791)
                     /locus_tag="NBH32_002071"
     CDS             complement(8075..8791)
                     /locus_tag="NBH32_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000688500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1515651.1"
                     /translation="MKIFICEDDPKQRENMVTIIKNYIMIEEKPMEIALATDNPYEVL
                     EQAKNMNDIGCYFLDIQLSTDINGIKLGSEIRKHDPVGNIIFVTSHSELTYLTFVYKV
                     AAMDFIFKDDPAELRTRIIDCLETAHTRLQLLSKDNSVETIELKRGSNSVYVQYDDIM
                     FFESSTKSHRLIAHLDNRQIEFYGNLKELSQLDDRFFRCHNSFVVNRHNIESIDSKER
                     IVYFKNKEHCYASVRNVKKI"
     gene            complement(8810..10054)
                     /locus_tag="NBH32_002072"
     CDS             complement(8810..10054)
                     /locus_tag="NBH32_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002448283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HCD1515652.1"
                     /translation="MILMFTIPAIISGIKYSKLDYFFIIVISTLSLFLFKMFDSASLI
                     ILTSFIIIMYFVKIKWYSILLIMTSQIILYCANYMYIVIYAYITKISDSIFVIFPSFF
                     VVYVTISILFSYIINRVLKKISTPYLILNKGFLIVISTILLLTFSLFFFYSQINSDEA
                     KVIRQYSFIFIGITIFLSILTFVISQFLLKEMKYKRNQEEIETYYEYTLKIEAINNEM
                     RKFRHDYVNILTTLSEFIREDDMPGLRDYFNKNIVPMKDNLQMNAIKLNGIENLKVRE
                     IKGLITAKILRAQEMNIPISIEIPDEVSSINLNMIDLSRSIGIILDNAIEASTEIDDP
                     IIRVAFIESENSVTFIVMNKCADDIPRIHELFQESFSTKGEGRGLGLSTLKEIADNAD
                     NVLLDTIIENGFFIQKVEIINN"
BASE COUNT         3532 a         1368 c         1836 g         3319 t
ORIGIN      
        1 attttattaa cttatgaaaa aagtcgtaat tattacatta ataaccattt tacagtataa
       61 aacgccgtct tgaaactaag atatttattt ttaaaattca ataatgtata atatttaggt
      121 attcattgta atcaattatt tgttttttaa taatctaaca attacgctct aactcgtttg
      181 aatacataga tataattcat tcaaacacga aggaggaaat agcgaatgag tgaccaagtt
      241 aaaattcctc gagcaacttt aaaacgtttg ccgttatatt atagatttgt cagttcatta
      301 aaatctaaag gtatagatcg tgtaaattca aaagcgatta gcgatgcgtt acaaattgac
      361 tcggcaacaa ttcgtcgtga cttttcatat tttggcgaat taggtaaaaa agggtacgga
      421 tataatatag atagtttatt ggatttcttt aaatctgaac taagcgagag tgacatgatc
      481 aaaatcgcaa ttgtcggagt tgggaaccta gggaaagctt tgctcacata taacttttca
      541 atacatgacg atatgacgat tacagaagcg tttgacgtaa aagaagatgt tattggccag
      601 aaaataggga acgttattgt aaaagataac gatgaattaa taacaacatt gaagaaggaa
      661 gaaatagatg ttgtgattct aactacacca gaaagagttg cacagaaagt tgcagatgaa
      721 ctcgtccaag ctggtgtgaa aggtatttta aacttcactc ctggtagaat taatacgcct
      781 tcagatgtgc aagtacatca aattgactta ggtatagaat tacagtcatt attattcttt
      841 atgaaaaatt atagtgaata aatgtgatgt tagctttgaa tgattaacct tgttatatct
      901 ttaattaatt atagagcagt ctgggactaa atatgtctca ggctttttct gttatgtttg
      961 aaaatgaaag gaaaaactgt tgtgttgata aatatagtat aggttaataa attaacacgt
     1021 agagatataa tgatgaatcg taattaatcg ccttccttat atgaatataa tataaatatc
     1081 aaaaatattg tgtataaggt tgaaaacaag aaaatcatca cctataatac atactaaact
     1141 tatcggaaat gagaggttag gattatgtgg atgattttta gtggcttaat tgtcggggga
     1201 ctcttaggtt ttgtcatgca aagaacaaga ttttgtttaa caggtggctt tcgagatatg
     1261 tatgtgcaaa agaataataa gatgttctat gcattattaa tcgctattac tattcaaagt
     1321 ataggattat tgattttgac ggcaacagat attttacaaa ttcctgcaca tagttttcca
     1381 atattgggaa caattatagg ttcttttatt tttggaattg gaatagtatt ggctggaggg
     1441 tgtgcaacag gtacttggta tcgcgctggt gaagggttaa ttggtagttg gattgcatta
     1501 gtattatatg ctgttactgc agcaatcact aaaacaggga ttttaaaacc agtaatggat
     1561 aaaattaatc aaccaacgaa tgtaaatagt gatatgtctc aaacaactgg cattccgttt
     1621 tggggattag tcgttatatt aactataatc accatttttc tagttgtaag aacactcaat
     1681 aataagaaag ttcgagtagc tgttccaaaa ctaaagcaac gttatacagg tattagatat
     1741 taccttttcg agaaacgata ccatccattt attgcagcaa ttgcaattgg acttatcgca
     1801 ctattagctt ggccaatgag tgcatcaact ggaagaaatg acggtttagg tataacaacg
     1861 ccatcagcaa atttagtaca ctttttgatt acaggtgaaa ctaaatttat tgattggggt
     1921 gtctttttag ttctagggat tttcattggt tcatatattg cagctagagg atcaagagaa
     1981 tttaaatggc gattgccaga caagattaca atacgaaaca gtgctattgg tggcatatgt
     2041 atgggatttg gtgcgtcagt tgctggtggt tgttctatcg gtaacggttt ggttgaaacg
     2101 gcaacgatga cttggcaagg atggattgcg ctagcatcga tgatagttgg tgtatggaca
     2161 atgagtcatt ttatctttgt tcgtccaatg aaaaagtaca acaacaatct gcaaaggtca
     2221 aacagcaaac gcaaatagta tagaagatta ttatacaaat gatgttgatc aaataaaagt
     2281 gattggaaaa ggagaaataa ttatgataca cgaattaggt acagtaggaa tggtatgtcc
     2341 atttccgtta attgaagcgc aaaagaaaat ggcaacattg caatctggag atgaattaaa
     2401 aattgatttt gattgcacgc aagcgacgga agctattcca aattgggcag cagaaaatgg
     2461 atatcctgta acaaactatg aacaaattga taatgcttca tggacaatta caattcaaaa
     2521 agtttaacgt tatcatttta acaataaaat agatgttaga gtttaggact acttccgcta
     2581 gtttaaaagc gagtaagtag tcttttttgt tttagttcat gaaattattt ttatatagtg
     2641 tggcatattt tattccaaaa gatgtaataa aacttaacgc atttttgctt tttataaatt
     2701 gtcagattat tatgaaaaca agggagtggt aagtatgaat cttaacgata cgatatttat
     2761 gtttttgtgt acattattag tttggctaat gacaccagga ttaagtttat tttatggtgg
     2821 gttagttcaa tctaaaaatg cgctaaatac tgtcatgcaa agtatggcag caattgtgct
     2881 tgttacattt gtatggataa cagttggttt tacaattagt tttggggaag ggaatttatg
     2941 gttcggaaat tgggaatata catttcttaa tcatgtaggt ttcgcgacac aagaagatat
     3001 tagcccacat attcctttcg ctttgtttat gttatttcaa atgatgtttt gtacgattgc
     3061 aatttctatt ttatctggtt caatcgctga gaaaatgaag tttattcctt atttattatt
     3121 cgtagtaata tggactgctc ttgtatacag tccagtagca cattgggttt ggggcggcgg
     3181 ttggattaac aaactcggtg tattagattt cgctggaggt acagttgttc atattacatc
     3241 aggtgtttct ggtttagtat tagctataat gattggaaaa ggaaacaaac attctgaatc
     3301 aacaccacat aatcttatca ttacgttgat tggcggtata ttcgtgtgga ttggttggta
     3361 tggatttaat gttggcagtg cttttacatt tgatcaaatt gcaatgcttg catttacgaa
     3421 tacagttatt tctgccagtg caggtgctat aggttggtta attttagaat atatttttaa
     3481 aaagacgaca agtttacttg gacttttact cggtgcatta gctggtttag ttgtcattac
     3541 acctgcagca ggatacgtga catatcttag tgcaacaata atggctttaa taggaggtat
     3601 ctgttgttat attgtcatca attacatcaa ggtaaaatta aagtatcatg atgcattaga
     3661 tgcatttggt atccacggtg ttggcggtat tattggtgct gttttaacag cagttttcca
     3721 aagtaaaaag gcaaatcctg acattgagaa tggctttatt tatactggtg acatacatat
     3781 tatacttgta caaatattat gtgtaacagc agttgtaatt tttagtatcg tcatgacgtt
     3841 tattattgcg aaagtaatta aattaattac accattatct gttacggaac aagaaacgaa
     3901 tataggatta gacaagattg ttcacggtga acacgcttac tttgaaggtg agctaaatag
     3961 attcaataaa catattcgat attagaatat atttacatag aatatttatt gtcctgacat
     4021 ttaactaaag gttgatgttg ggacattttg ttatacaaaa gttttatttt gaaatctttt
     4081 tatgaaagaa gcagaaatat tatttaaagc ggttacacat atgctaaaat aaggctaagt
     4141 gtcacaaata atgataggtg aataagtatg aaaaatatat ctgatattgc caaattggca
     4201 ggcgtttcaa aaagtacagt atctagattt ttaaataatg gatctgtcag taaaaaaaca
     4261 agtgaaaaat taacaaaaat tatagcagaa catgactatc aaccgaatca atttgctcaa
     4321 agtttaagag cgagacaaac acaccttatt ggtgccatta tacctagaat gaattcatat
     4381 gcagtagatg aaacaatcaa aggattggca aaacaatgcc aaaaatatga atcacaatta
     4441 attttaaatt atacaggttt aaatatcgaa gcagaaatac aagcgcttga aacattagca
     4501 cgcagtaaag tagatggtat tgttttaatg gctacagaca taacagagag acatatagaa
     4561 gtcattaaca aaatgaatgt accaatcgtt attgttggtc aacaacatga acaacttcat
     4621 agtattgtgc atgatgatta taaagcaggt caaattatag gtgaatggat tggtcaacag
     4681 ggatatcaac aaattgaagt gtttagtgtg agtgaaaaag atattgcagt tggtatacat
     4741 agaaaacgtg gtttacttga ccagttagct aaataccaaa ttaaacctaa tatacatgaa
     4801 acaaatttta cttatgtgga agcacaaaaa gatgttgcaa atgttttgga aaatgtggag
     4861 caagtagatg cggttgttgg agcaactgat acgattgcat tagctgccta taaatattat
     4921 tctgataaaa aagaagttat gaaaccacat caaatatatg gttttggtgg tgatccaatg
     4981 acacaattag tgtctccatc gataaaaaca attcattata attattttga agctggccaa
     5041 tgcgcgatgg aagagataca acagatgctt aaaaagcaag atatgccata tagcgtcaca
     5101 gtagatgtta atatttagac gctgtatttt ttaaaataaa tgtggaaccg ataccatata
     5161 actataaatg gataggttaa aagttaaaga acgtaggtaa aatttgctat aatagaatat
     5221 aaattgttaa cagcataaat tataaaagga ggactgggta aatattatga ccgaatggac
     5281 tagagaagaa cgttatcaac gaatcgagga cgttgatact gagtatttta aaacattaaa
     5341 acaacaagtt gatcaatcaa aatttcgtca acaatttcat atacaaccag aaacaggctt
     5401 attaaatgat cccaacggac ttatttttta taaagggaag tattatgttt cacatcaatg
     5461 gttcccttta ggcgcagtac atggcttaaa gtattggtat aactacacga gtgatgactt
     5521 aataaacttt aaagctgaag ggccaatttt aaatccagat actaaatatg acagccatgg
     5581 tgtttatagc ggtagcgctt ttgaatttaa cgggcattta tattatatgt acacaggaaa
     5641 tcatcgagat aatcattggc aacgacatgc gagtcagatg atcgcacgat tgaaagaaga
     5701 cggttcagta gaaaagtttc caaagccagt aattagccag caaccagaag gatatacaag
     5761 tcattttaga gatcctaaag tttttaaata tggtgagaaa tattatgcga tcattggcgc
     5821 acaaaataat gatcagcaag gtcgattatt actttataat actgaagata taattaattg
     5881 gcattattta ggtgaaataa atacagagtt agatgatttt ggatatatgt gggaatgccc
     5941 agattacttt aatgtagata atcaagatgt catacttatt tgtccacaag gtattgaacc
     6001 aaaaggcgat cagttcaaaa atatttatca aagtggttat atacttggaa agtttgatat
     6061 tgaaaaatta acatatgaac atgaaaattt tgtcgagctt gataatggct ttgatttcta
     6121 tgcacctcaa acatttttag atgaaaaagg ccgacgagta ctaattggat ggatggggtt
     6181 accggaaatc gaatatccta ctgataatga aggatgggcc cattgcttaa caattcctcg
     6241 tgtattaaat gtagaaaatg gtcaacttaa gcaacgtcca tatccagcgt tggaaaaatt
     6301 acgtcataat aaagagacag cattaggcta cgcaaataaa tttactcgaa aattacatcc
     6361 gtatgaaggt aaacagtatg aattaatcat agatattttg gataatgatg ctaccgaagt
     6421 gtactttgaa ttacgtacgt ctaagacttc ttcaacatta atcgcttata acaagcgtga
     6481 aaataaaata atattagatc gcagcgacag tggtttattg cctacaaatg ttgaaggtac
     6541 gacgcgtagt acgatattag aaacgccatt aaagcaatta caaatttttg ttgatacatc
     6601 tagtatagaa attttctgta atgatgggga acgtgttttg acatctcgaa ttttcccgac
     6661 agaagacgca ctaggtatta aaacatcaac agaatcaggg caagtatatt tacaatttac
     6721 taaatatgat ttaaaggatg aacataaatg agacgtctat tttcaatagg agaagcgtta
     6781 attgatttta ttccaaatat tacaaatgcg aatttaaaag atgttcaaac atttacgaag
     6841 caaattggtg gcgcgccatg caatgtagct tgtacagttc aaaagttagg acaacaagcg
     6901 tatatgatta cacaattagg taatgatgca tttggagata gcattattga aacgatatca
     6961 agtattggtg tagatgtatc aaaagtgtat cgcacgaatg aagcaaacac tgcgttagct
     7021 tttgtgagtt tgactgaagc gggcgagcga gatttttctt tttatcgtaa gccctctgct
     7081 gatatgttat ttgaaccaag ttttgtgaat gatattgatg tgaatgaaaa tgatgtagta
     7141 catttttgtt ctgttgattt gatcgatagt ccgatgagag aagcacatta tcaattgatt
     7201 accaaaacat taaatgcaaa tggtactgtc gtatttgatc ctaatgtaag attgccatta
     7261 tgggataacg ctgaagattt aagacaaact attcatacct ttttgccatt ggcgcatatt
     7321 gtaaaagttt cagatgaaga attagaattt attacaggta ttcatgatga aaataaagcc
     7381 attcaatcat tatttacagg taatgtcact gtagtcattt atacgaaggg agcagatggt
     7441 gctgccgtat atttgaaaaa tggtattaat cattatcata gtggctataa ggtaaaacct
     7501 gttgatacaa ctggggcagg ggatgccttt attggcgcag ttattagcag gattttagca
     7561 accgatgtgt taaatttaac tcagttattt gaaaatgaag gtgaggaaat tttagcgttt
     7621 agcaatcgcg ttgctgcaat agtgacaacg aagtatggtg ctattaatag tttgccaact
     7681 ttacaagagg ttgaacaagc attttaaata attcatttat aaaacagtgt aggaaacaag
     7741 ttgaatcttg tgctacactg ttttttaaaa caaaaataag attcacgggg taggaaattg
     7801 tagtatttta tttaagaaaa ctgtcgattt caaatttagt atattttgtt aaaaattgcg
     7861 ctataggatt gtagagtgaa atagaaattg ataaattatg tagtaatagg aataggtata
     7921 caaagagacg ttacatcatt ttttaattca cgcgtcatat ttaattttgc tattaaaatt
     7981 aaaaagccag ctatacagtg catttgctag ttatcttgtt aaaatccaac aagatttaca
     8041 tttgaatacg ccgttaactg actttattat cttattatat ttttttaacg tttctcaccg
     8101 atgcatagca gtgttcttta tttttaaaat agacaatgcg ctctttcgaa tctatagatt
     8161 cgatattatg gcgattgacg acaaagctat tatgacatct aaagaaacga tcatctaatt
     8221 gactcagttc ttttaaatta ccataaaatt caatttgacg gttatctaaa tgggcaatga
     8281 gtctgtgaga ttttgttgat gattcaaaaa acataatatc atcatattga acatacactg
     8341 aattactgcc acgttttaat tcaatcgttt caacgctatt atctttagac aacaattgta
     8401 agcgtgtatg tgcagtttct aaacaatcta taatacgtgt tcttaattct gctggatcat
     8461 ctttaaaaat aaaatccatc gctgcaactt tgtagacaaa tgttaaatac gtaagttcac
     8521 tatgactcgt aacgaaaata atgttaccaa ctgggtcatg cttacggatt tcactgccta
     8581 atttgatacc attaatatca gttgaaagtt gaatatctaa aaagtaacag cctatgtcat
     8641 tcatattttt agcttgctca agcacctcat aaggattatc agttgcgagg gcaatttcca
     8701 taggcttttc ttctatcatt atataatttt taataatggt aaccatgttt tctctttgtt
     8761 ttggatcgtc ttcgcaaatg aaaattttca tacattcaca tccttatggc tagttgttaa
     8821 taatttcaac tttttgaata aagaaaccat tttcgataat tgtatctaat aagacattgt
     8881 ctgcattatc agcaatttct tttaaagttg atagacctaa accacgacct tcacctttag
     8941 tagaaaaact ttcttggaac aattcatgaa tgcgtggtat atcatcagcg catttattca
     9001 taacaataaa cgttactgaa ttttcacttt caataaatgc aacgcgaatg atagggtcat
     9061 caatttcagt tgatgcctca attgcattat caagaataat accaatactg cgacttaaat
     9121 cgatcatatt caagttaatg ctacttactt catcgggtat ttctatacta atcggaatat
     9181 tcatttcttg tgcacgtaaa attttcgcag taattaagcc tttaatttca cgtactttaa
     9241 gattctcgat accatttaat tttatagcat tcatttgtaa attgtctttc ataggtacaa
     9301 tatttttatt gaaataatca cgtaggccag gcatgtcatc ttctcgaatg aattctgaaa
     9361 gcgttgttaa gatattgaca taatcgtgac ggaacttgcg catttcgttg ttgatagctt
     9421 caatcttcaa tgtatattca taataggttt caatttcttc ttgattacgt ttatatttca
     9481 tctctttaag gagaaattga gaaataacaa atgttaatat acttaaaaat atagtgatac
     9541 caataaaaat aaaagaatac tgccttatta ctttagcttc atccgagttt atttgtgaat
     9601 aaaagaaaaa taatgaaaaa gtaagcagta agatagtcga aataactatt aaaaatcctt
     9661 tgtttagtat tagatatggt gtgctaattt ttttgagaac tctatttatt atatatgaga
     9721 atagtatact aatagtcaca taaactacaa aaaagctagg gaatattaca aatatactat
     9781 cagaaatttt ggtgatatat gcatatataa ctatatacat gtagttagca cagtacagaa
     9841 taatctgcga agtcataatc aacaaaatag aataccattt gattttgaca aaatacatta
     9901 taataataaa tgaagttaat atgattaagg acgcgctatc aaacatttta aatagaaata
     9961 acgataatgt cgaaattact atgatgaaaa aataatcaag tttactgtac ttaataccac
    10021 taattatagc tggtattgta aacattaata tcatt
//
LOCUS       DAHHOJ010000095        10028 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.095, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000095 DAHHOJ010000000
VERSION     DAHHOJ010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 10028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10028
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.095"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..509
                     /locus_tag="NBH32_002073"
     CDS             <1..509
                     /locus_tag="NBH32_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515653.1"
                     /translation="SAIGSIGTFIMALFYFVSVSVQLYQMKISFLPALGFNQILLERE
                     GDQLNIMNSATEEHHHKDYIKLYNLGGGAAKKIAIEVLLGNDKVIQKKYVNILPSKEG
                     YMLPINKNVYEELERTIENNGYEADLNVRMTYYHNVSRKQQEVILKGQIDRFNTYNNK
                     EIYDLQFI"
     gene            648..1178
                     /locus_tag="NBH32_002074"
     CDS             648..1178
                     /locus_tag="NBH32_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1515654.1"
                     /translation="MQIRQIHQHDFAQVDQLIRTAFENSEHGYGNESELVDQIRLSDT
                     YDNNLEIVAVLQNEVVGHGLLSEVYLDNGAQREIGLVLAPVSVDIHHQNKGIGKRLIQ
                     ALEREAILKGYNFISVLGWPTYYANLGYQRASMYDIYPPYDGIPDEAFLIKELKVNSL
                     AGKTGTINYTSAFEKI"
     gene            1441..4200
                     /gene="clfA"
                     /locus_tag="NBH32_002075"
     CDS             1441..4200
                     /gene="clfA"
                     /locus_tag="NBH32_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MSCRAMM family adhesin clumping factor ClfA"
                     /protein_id="HCD1515655.1"
                     /translation="MNMKKKEKHAIRKKSIGVASVLVGTLIGFGLLSSKEADASENSV
                     TQSDSASNESKSNDSSSVSAAPKTVDTNVSDTKTTSNTNSGETSVAQNPAQQETTQSA
                     STNATTEETPATGEATTTATNQANTPATTQSSNTNAEELVNQTNNETTSNDTNTVSSV
                     NSPQNSTNAENVSTTQDTSTEATPSNNESAPQSTDASNKDVVNQAVNTSAPRMRAFSL
                     AAVAADAPAAGTDITNQLTDVKVTIDSGTTVYPHQAGYVKLNYGFSVPNSAVKGDTFK
                     ITVPKELNLNGVTSTAKVPPIMAGDQVLANGVIDSDGNVIYTFTDYVDNKENVTANIT
                     MPAYIDPENVTKTGNVTLTTGIGTNTASKTVLIDYEKYGQFHNLSIKGTIDQIDKTNN
                     TYRQTIYVNPSGDNVVLPALTGNLIPNTKSNALIDAKNTDIKVYRVDNANDLSESYYV
                     NPSDFEDVTNQVRISFPNANQYKVEFPTDDDQITTPYIVVVNGHIDPASTGDLALRST
                     FYGYDSNFIWRSMSWDNEVAFNNGSGSGDGIDKPVVPEQPDEPGEIEPIPEDSDSDPG
                     SDSGSDSNSDSGSDSGSDSTSDSGSDSASDSDSASDSDSASDSDSASDSDSASDSDSA
                     SDSDSASDSDSASDSDSASDSDSASDSDSASDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSNSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSESDSDSDSGSNNN
                     VVPPNSPKNGTNASNKNEAKDSKEPLPDTGSEDEANTSLIWGLLASLGSLLLFRRKKE
                     NKDKK"
     gene            4421..5929
                     /gene="vwb"
                     /locus_tag="NBH32_002076"
     CDS             4421..5929
                     /gene="vwb"
                     /locus_tag="NBH32_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000791704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor binding protein Vwb"
                     /protein_id="HCD1515656.1"
                     /translation="MKNKLLVLSLGALCVSQIWESNHASAVVSGEKNPYVSKALSIKG
                     QKTNSWNLGQYKDSLRTVMCTSEINKNDGYDEPEYKEAMDTYRKKLFAELDALNKFLD
                     EERKIESSKKNNNQVANGVLGLTHQRYLAIHEAIKENKNEFERKTQDIESRNPDLKKF
                     DRDKDYEVRVKLNELENKVLMLGHAFPNKHEARENLYNKLDLIVGRSDDEREERHPQN
                     DRLSKERVEDLESIIDEFFVEINENRPLNIPALIESNEENIVMAKKLKADTEEAKANT
                     SKRSKRSLNTQNHKSKINEVTEEQKANYEKKFKEIKERFLAKQKNKNNTPVVSLEYDE
                     DDNENDKKLVVSEPSKKPTTPPTYTETTTQVPMPTVERQTQQQIIYNAPKQLAGLNGE
                     SHDFTTTHQSPTTSNHTHNHLIEFEETSALPGRKSGSLVGISQIDSSHLTEREKRVIK
                     REHVREAQKLVDNYKDTHSYKDRLNAQQKVNTLSEGHQKRFNKQINKVYNGK"
     gene            6282..7304
                     /gene="emp"
                     /locus_tag="NBH32_002077"
     CDS             6282..7304
                     /gene="emp"
                     /locus_tag="NBH32_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular matrix protein-binding adhesin
                     Emp"
                     /protein_id="HCD1515657.1"
                     /translation="MKKKLLVLTMSTLFATQIMNSNHAKASVTESVDKKFVVPESGIN
                     KIIPAYDEFKSAPKVNVSNLTDNKNFVVSEGKLNKISDPSAASKIVDKNFVVPESKLG
                     NIVPEYKEINNRVNVATNNPASQQVDKHFVAKGPEVNRFITQNKVNHHFITTQTHYKK
                     VITSYKSTHVHKHVNHAKDSINKHFIVKPSESPRYTHPSQSLIIKHHFAVPGYHAHKF
                     VTPGHASIKINHFCVVPQINSFKVIPPYGHNSHRMHVPSFQNNTTATHQNAKVNKAYD
                     YKYYYSYKVVKGVKKYFSFSQSNGFKTGKPALNIKNVNYQYAVPSYSPTHVVPEFKGI
                     LPAPRV"
     gene            7634..8142
                     /locus_tag="NBH32_002078"
                     /pseudo
     CDS             7634..8142
                     /locus_tag="NBH32_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001627793.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8483..9169
                     /locus_tag="NBH32_002079"
     CDS             8483..9169
                     /locus_tag="NBH32_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD1515658.1"
                     /translation="MTEYLLSAGICMAIVSILLIGMAISNVSKGQYAKRFFFFATSCL
                     VLTLVVVSSLSSSANASQTDNGVNRIGSEDSTVYSATSTKKLHKEPATLIKAIDGDTV
                     KLMYKGQPMTFRLLLVDTPETKHPKKGVEKYGPEASAFTKKMVENAKKIEVEFDKGQR
                     TDKYGRGLAYIYADGKMVNEALVRQGLAKVAYVYKPNNTHEQLLRKSEAQAKKEKLNI
                     WSEDNADSGQ"
     gene            9526..9726
                     /locus_tag="NBH32_002080"
     CDS             9526..9726
                     /locus_tag="NBH32_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCD1515659.1"
                     /translation="MNNGTVKWFNAEKGFGFIEREDGSDVFVHFSAIAEDGYKSLEEG
                     QKVEFDIVEGDRGEQAANVVKM"
BASE COUNT         3887 a         1466 c         1780 g         2895 t
ORIGIN      
        1 tttcagctat aggatctatt gggacattta ttatggcttt attttatttt gtatcagttt
       61 cagttcaact ttatcaaatg aaaattagct ttctgccagc tttaggtttt aaccaaattt
      121 tattagaaag ggagggtgat caacttaata taatgaattc ggcaacagaa gagcatcatc
      181 ataaagatta tattaaacta tataatttag gtggcggtgc tgctaaaaaa attgcaatag
      241 aggttttatt gggtaatgat aaagtcattc agaaaaaata cgtgaatatt ttacctagta
      301 aagaagggta catgttacca attaataaaa atgtgtacga agaattagaa agaacgattg
      361 agaacaatgg ttatgaagct gatttgaatg tacgtatgac ttattatcat aatgtaagtc
      421 gcaaacaaca ggaagttata ttaaaaggtc aaatcgaccg ttttaatact tataataata
      481 aagaaattta tgatttgcag tttatctaaa aattgattta agagggtagt tgtttattgc
      541 gaaaaatatc attcaatttt aatgaaataa tggcgtcatt actataaaat attactttat
      601 gttgtaaagc atttttctat aagatagaac taaaaggagg ggcaaagatg caaattagac
      661 aaatacatca acatgacttt gctcaagttg accagttaat tagaacggca tttgaaaata
      721 gtgaacatgg ttatggtaat gaatcagagc tagtagacca aattcgtcta agtgatacgt
      781 atgacaataa cttagaaata gtagctgttc ttcaaaacga agttgtaggg cacggtttac
      841 taagtgaagt ttatcttgat aacggagcac aacgggaaat tggattagtg ttagcacctg
      901 tatctgttga tattcatcat caaaataaag gtatagggaa gcgattgatt caagcattag
      961 aacgagaagc aatattaaaa ggatataatt ttatcagtgt attaggatgg ccgacgtatt
     1021 atgccaatct aggatatcaa cgcgcaagta tgtacgacat ttatccacca tatgatggta
     1081 taccagacga agcgttttta attaaagaat taaaagtgaa cagtttagcg ggaaaaacag
     1141 gtaccataaa ttacacatct gcttttgaaa aaatatgatt tcaagatagg attacattag
     1201 gcagagttca tattaataat acaaattgtt cgcaatgaaa tcgcacgttg tcgtttgcaa
     1261 tttataaaat agcaataaat aaaatgtttg ttaggaaagt attattgtgg ataataaaaa
     1321 atcgatacaa attaattgct ataatgcaat ttcagtgtat aattgcatta atggagatta
     1381 aatatatctt taaagggtat acagttaata taaaatgact ttttaaaaag agggaataaa
     1441 atgaatatga agaaaaaaga aaaacacgca attcggaaaa aatcgattgg cgtggcttca
     1501 gtgcttgtag gtacgttaat cggttttgga ctactcagca gtaaagaagc agatgcaagt
     1561 gaaaatagtg ttacgcaatc cgatagcgca agtaacgaaa gcaaaagtaa tgattcaagt
     1621 agcgttagtg ctgcacctaa aacagtcgac acaaacgtga gtgatactaa aacaacgtca
     1681 aacactaata gtggcgaaac aagtgtggcg caaaatccgg cacaacagga aacgacacaa
     1741 tcagcatcaa caaatgcaac tacggaagaa actccggcaa ctggtgaagc tactactacg
     1801 gcaacgaatc aagctaatac accggcaaca actcaatcaa gcaatacaaa tgcggaggaa
     1861 ttagtgaatc aaacaaataa tgaaacgact tctaatgata ctaatacagt atcatctgta
     1921 aattcacctc aaaattctac aaatgcggaa aatgtttcaa caacgcaaga tacttcaact
     1981 gaagcaacac cttcaaacaa tgaatcagct ccacagagta cagatgcaag taataaagat
     2041 gtagttaatc aagcggttaa tacaagtgcg cctagaatga gagcatttag tttagcggca
     2101 gtagctgcag atgcaccggc tgctggcaca gatattacga atcagttgac agatgtgaaa
     2161 gttactattg actctggtac gactgtgtat ccgcaccaag caggttatgt caaactgaat
     2221 tatggttttt cagtccctaa ttctgctgtt aaaggtgaca cattcaaaat aactgtacct
     2281 aaagaattaa acttaaatgg tgtaacttca actgctaagg tgccaccaat tatggccgga
     2341 gatcaagtat tggcaaatgg tgtaatcgat agtgatggta atgttattta tacatttaca
     2401 gactatgttg ataataaaga aaatgtaaca gctaatatta ctatgccagc ttatattgac
     2461 cctgaaaatg ttacaaagac aggtaatgtg acattgacaa ctggcatagg aaccaatact
     2521 gctagtaaga cagtattaat cgactatgag aaatatggac aattccataa tttatcaatt
     2581 aaaggtacga ttgatcaaat cgataaaaca aataatacgt atcgccaaac aatttatgtc
     2641 aatccaagcg gagataacgt tgtgttacct gccttaacag gtaatttaat tcctaataca
     2701 aagagtaatg cgttaataga tgcaaaaaac actgatatta aagtttatag agtcgataat
     2761 gctaatgatt tatctgaaag ttattatgtg aatcctagcg attttgaaga tgtaactaat
     2821 caagttagaa tttcatttcc aaatgctaat caatacaaag tagaatttcc tacggacgat
     2881 gaccaaatta caacaccgta tattgtagtt gttaatggcc atattgatcc tgctagtaca
     2941 ggtgatttag cactacgttc gacattttat ggttatgatt ctaattttat atggagatct
     3001 atgtcatggg acaacgaagt agcatttaat aacggatcag gctctggtga cggtatcgat
     3061 aaaccagttg ttcctgaaca acctgatgag cctggtgaaa ttgaaccaat tccagaggat
     3121 tcagattctg acccaggttc agattctggc agcgattcta attcagatag cggttcagat
     3181 tcgggtagtg attctacatc agatagtggt tcagattcag caagcgattc agattcagca
     3241 agtgattcag attcagcaag tgattcagac tcagcaagtg attcagattc agcaagtgat
     3301 tcagattcag caagtgattc agactcagca agtgattcag attcagcaag tgattcagac
     3361 tcagcaagtg attcagattc agcgagcgat tcagattcag cgagtgattc agattcagac
     3421 agcgattcag actcagacag tgactcagat tccgatagtg actcagactc agatagcgac
     3481 tcagattccg atagtgactc cgactcagac agcgattcag attcagatag tgattcagat
     3541 tcagatagcg attcggattc agatagtgac tcagattcag atagcgactc agattcagat
     3601 agcgattcag attccgacag tgactcagat tccgacagcg attcagattc cgacagtgac
     3661 tcagactcag acagtgattc ggattcagat agcgattcag actcagacag tgactcggat
     3721 tcagatagcg attcagactc agacagtgac tcggattcag atagcgattc caactcagac
     3781 agtgattcag actcagacag cgattcagat tccgacagtg actcagactc agacagcgat
     3841 tccgactcag acagtgactc ggattcagat agcgattctg actcagatag tgactccgac
     3901 tcagacagtg actcagattc agatagcgat tcagattccg acagtgactc agattcggat
     3961 agcgattcag aatcagatag tgactccgac tcaggttcta acaataatgt agtgccgcct
     4021 aattcaccta aaaatggtac taatgcttct aataaaaatg aggctaaaga tagtaaagag
     4081 ccattaccag atacaggttc tgaagatgaa gcgaatacgt cactaatttg gggattatta
     4141 gcatcattag gttcattact acttttcaga agaaaaaaag aaaataaaga taagaaataa
     4201 gtaataatga tattaaatta ataatatgat tcatgaagaa gccaccttaa aaggtggctt
     4261 ttttacttgg attttccaaa tatattgttt gaatataatt aataattaat tcatcaacag
     4321 ttaattattt taaaaaggta gatgttatat aatttggctt ggcgaaaaaa tagggtgtaa
     4381 ggtaggttgt taattaggga aaattaagga gaaaatacag ttgaaaaata aattgctagt
     4441 tttatcattg ggagcattat gtgtatcaca aatttgggaa agtaatcatg cgagtgcagt
     4501 ggtttctggg gagaagaatc catatgtatc aaaagcttta tcaattaaag gtcaaaagac
     4561 aaattcatgg aatttaggtc aatataaaga tagtcttcga acagttatgt gcacatcaga
     4621 aattaataaa aatgatggat atgatgaacc tgaatataaa gaggcgatgg atacttaccg
     4681 aaaaaaatta tttgctgaat tagatgcgct taacaagttt ttagatgaag agagaaaaat
     4741 agaaagtagt aaaaaaaata acaaccaagt tgcaaatggt gtattgggct taacacatca
     4801 aaggtattta gctattcatg aagcaataaa ggaaaataaa aatgagtttg aaagaaagac
     4861 acaagatatc gaaagtagaa atcctgattt gaaaaaattt gatagagata aagattacga
     4921 agttagagta aaattaaatg aactagaaaa taaagtatta atgcttggac acgcttttcc
     4981 aaataaacat gaagcaagag agaatcttta taataaatta gatttgattg ttggccgtag
     5041 tgatgatgag agagaagaaa ggcatcctca aaatgatagg ttatctaaag aacgagttga
     5101 agatttagaa tcaattattg atgaattttt tgttgaaatc aatgaaaata ggccgttgaa
     5161 tataccggcg ttaattgaaa gtaatgaaga aaatatagta atggcaaaaa aactaaaagc
     5221 agatacagaa gaagctaaag cgaatacatc taaaagaagt aaaagaagtt taaatactca
     5281 aaatcacaaa tctaaaataa acgaagtaac tgaagaacaa aaagctaatt atgaaaagaa
     5341 atttaaagaa ataaaagaac gatttttagc taaacaaaaa aataaaaata acacaccagt
     5401 tgtatcgtta gaatatgacg aagacgacaa cgaaaacgac aagaaacttg tggtttctga
     5461 gccatcaaag aaaccaacaa caccgcctac atatactgaa acaacgacac aggtacctat
     5521 gcctacagtt gagcgtcaaa ctcagcaaca aattatttat aatgcaccaa aacaattggc
     5581 tggattaaat ggtgaaagtc atgatttcac aacaacgcat caatcaccaa caacttcaaa
     5641 tcatacgcat aatcatctta ttgaatttga agaaacgtct gctttacctg gtagaaaatc
     5701 aggatcactg gttggtataa gtcaaattga ttcttctcat ctaactgaac gtgagaagcg
     5761 tgtaattaag cgtgaacacg ttagagaagc tcaaaagtta gttgataatt ataaagatac
     5821 acatagttat aaagaccgat taaatgcaca acaaaaagta aatactttaa gtgaaggtca
     5881 tcaaaaacgt tttaataaac aaatcaataa agtatacaat ggcaaataat attaatgcat
     5941 ggctgcaaag caaataatga gtttgtcgta aaaatcacaa cattttaaac tagcaataaa
     6001 taatatcaaa gtcatcaatt caatgatgca atctagtata gtccacattc taaacaggtg
     6061 tggactatta cttttttcac tttatattac gaaaaaatta ttatgcttaa ctatcaatat
     6121 caataattaa ttttaagctg aaaaacaata aaaatgttaa gacaacgttt acttcaagtt
     6181 aattattata ctgaaaattc tggtatataa tgctgttagt gaatataaca ggaaaattaa
     6241 attggttata atattgagtc tatataaagg agaaataaca gatgaaaaag aaattattag
     6301 ttttaactat gagcacgcta tttgctacac aaattatgaa ttcaaatcac gctaaagcat
     6361 cagtgacaga gagtgttgac aaaaaatttg tagttccaga atcaggtatt aataaaatta
     6421 ttccagctta cgatgaattt aagagtgcac caaaagtaaa tgttagtaat ttaactgaca
     6481 ataaaaattt tgtagtttct gaaggcaaat tgaataagat ttcagatcct tcagcagcaa
     6541 gtaaaattgt agataaaaac tttgtcgtac cagaatcaaa gttaggaaac attgtgccag
     6601 agtacaaaga aatcaataat cgcgtgaatg tagcaacaaa caatccagct tcacaacaag
     6661 ttgataagca ttttgttgct aaaggcccag aagtaaatag atttattacg caaaacaaag
     6721 taaaccacca cttcattact acgcaaaccc actacaagaa agttattact tcatacaaat
     6781 caacacatgt acataaacat gtaaatcatg caaaggattc tattaataaa cactttattg
     6841 ttaaaccatc agaatcgcct agatatacac atccatctca atctttaatt atcaagcatc
     6901 attttgcagt tcctggatat cacgcgcata aatttgttac accagggcat gctagcatta
     6961 aaattaatca cttttgtgtt gtgccacaaa taaatagttt caaggtaatt ccaccatatg
     7021 gtcacaattc tcaccgtatg catgtaccaa gtttccaaaa taacacaaca gcaacacatc
     7081 aaaatgctaa agtaaataaa gcatatgact ataaatacta ctattcttat aaagtagtta
     7141 aaggtgtgaa aaaatatttc tcattttcac aatctaatgg ttttaaaact ggtaaaccag
     7201 cattaaatat caaaaatgta aattatcaat atgctgttcc aagttatagc cctacacacg
     7261 ttgttcctga atttaagggc attttaccag caccacgagt ataaaaattg gcactaagtt
     7321 tacgagatat gataaatacc tattatttta aatatagtct gcaatctatg aggttgtagg
     7381 ctatgttttt tgcaatttag caatcaacac ccatcaacaa attataccgt ttttctactt
     7441 tcaaagttgg aagtaacata atcttaaata aatatattat taattaagat aaatataaga
     7501 ctcaagatta ttgttaatag tttgttcatc gcaagttaat tattatttct aaaaaattgg
     7561 tatataattt cgatggcgaa gaaaacaggg tgaaaaagtc ggttttacaa tcaatgcaaa
     7621 taaggagtaa aaaatgaaaa ggaaagtact agtattaaca atgggcgtac tttgtgcgac
     7681 gcaattatgg cattctaatc acgcaaacgc attagtgaca gagagtgttg aaactaattt
     7741 tgttgtaaaa gattcgggta ataataatat tttacaaacc catactgaaa ttactactga
     7801 ggagaaattt tcaatagtag aaaagagcca attaaataca ctaaaatcac tatctaatga
     7861 caactacata gaatatgatt tacatactaa tcaaacaggc attaaaaaag gttggttata
     7921 tggctatagt gaaattgact catcacattt cacagactgt gacaaacgcg ctattagacg
     7981 tgatcatgtt aaagaagcac aaagcttaat taacgattat aaagatacac aaagttatga
     8041 agatctcgct aaggcaacac acttagtcag tctcatcaaa attatttgaa taaacaaatt
     8101 gataaagtga ataataagat agagaaaact gaaaaacgct aatccaaagt aaattataag
     8161 ttatacatct cgtttttaaa tgacaattta tccccgtaaa tattataaat aatcttttca
     8221 aattccacat agatatagag acactaataa acctctttgt ctcgatatga tagtctgcaa
     8281 cgattcatgt tgtaggcttt ttaattttac aaataaggct aaatatataa gttctgacac
     8341 ctaaaatata gaaaatacat aaaagtaagt atagttattt tattataatt attaaatttt
     8401 tattaattaa ttgtaaaaat gtagaattat aattaattaa cgtttaatat taaaattaac
     8461 taaaaagaaa gaggtgttag ttatgacaga atacttatta agtgctggca tatgtatggc
     8521 aattgtttca atattactta tagggatggc tatcagtaat gtttcgaaag ggcaatacgc
     8581 aaagaggttt ttctttttcg ctactagttg cttagtgtta actttagttg tagtttcaag
     8641 tctaagtagc tcagcaaatg catcacaaac agataacggc gtaaatagaa ttggttctga
     8701 agattcaaca gtatatagtg caacttcaac taaaaaatta cataaagaac ctgcgacatt
     8761 aattaaagcg attgatggtg atacggttaa attaatgtac aaaggtcaac caatgacatt
     8821 cagactatta ttagttgata cacctgaaac aaagcatcct aaaaaaggtg tagagaaata
     8881 tggccctgaa gcaagtgcat ttacgaaaaa aatggtagaa aatgcaaaga aaattgaagt
     8941 cgagtttgac aaaggtcaaa gaactgataa atatggacgt ggcttagcgt atatttatgc
     9001 tgatggaaaa atggtaaacg aagctttagt tcgtcaaggc ttggctaaag ttgcttatgt
     9061 ttataaacct aacaatacac atgaacaact tttaagaaaa agtgaagcac aagcgaaaaa
     9121 agagaaatta aatatttgga gcgaagacaa cgctgattca ggtcaataat gctcattgta
     9181 aaagtgtcac tgctgctagt ggcactttta taatttttag atcacgatat gatttattat
     9241 caattcagaa ttaaaaaagt aaatagtatc aaaagcaagt gtatttaata ttagaaaata
     9301 aaaattttaa atttagtatt aaaatggaat attactatat atttcaatgt gtattatcac
     9361 agaaaataaa ataatgcttt acttctatat ttaaaagtgt ataatgaaag ttaagtaata
     9421 aagagcgtga agaaaaatgt gagttattta tatagaatat tctccttttc atttatgaat
     9481 ttgttacaaa atatttagtg caaaagcacg acggaggtat tcaatatgaa taacggtaca
     9541 gttaaatggt ttaatgcaga aaaaggtttt ggtttcatcg aaagagaaga tggtagcgac
     9601 gtattcgtac acttctcagc aatcgctgaa gatggataca aatcattaga agaaggccaa
     9661 aaagttgaat tcgacatcgt tgaaggcgac cgtggcgagc aagctgcaaa cgtagttaaa
     9721 atgtaatctt aactttttca aacagtcctt agtatagggc tgttttttta tgctttaaat
     9781 cgataacagt tggtgtggta aaagcactag ctgttacttt tttgtccaat aaatataggt
     9841 ggagatctaa caatatataa tggttctaaa ataaatcgaa ctgatggaaa agttttttac
     9901 ttttcatctg tccgactttt gattttgaat aaaaaaaagc gccaatacag aactttaata
     9961 atgacgagaa ttaaagtctg tatatggcga taacaagaag taatgttaaa cactcaaaat
    10021 gtttaaca
//
LOCUS       DAHHOJ010000096         9901 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.096, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000096 DAHHOJ010000000
VERSION     DAHHOJ010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9901)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9901)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9901
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.096"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            807..1286
                     /locus_tag="NBH32_002081"
     CDS             807..1286
                     /locus_tag="NBH32_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515660.1"
                     /translation="MKITKTIGSLVLTAVLLTSVSPLTNANEIQTNNTKTAHQSYENI
                     NGVKINRNPNLPMNPEAQQRIGGTKVVKTAIKLIINNRGKAKNVIQQVGGKKAATIFD
                     KGYSGIVKELKPLLKWSEVPANAVGDAVKRGAINAGLSKGDAGIISTVVKEAIAWLF"
     gene            1308..1700
                     /locus_tag="NBH32_002082"
     CDS             1308..1700
                     /locus_tag="NBH32_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000888757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515661.1"
                     /translation="MLEDNGDIRLLYQSIEELASVLGQSQFETKTVSLIFVQMDLRYS
                     VFEKVQTDFMKYLAHKNSDEIKYIDLIKIIENALPEDKELSINVINSIIIGFANNYYP
                     ILQSLVPEIQSSYGITINKNFNIDSIDF"
     gene            complement(1940..3481)
                     /gene="guaA"
                     /locus_tag="NBH32_002083"
     CDS             complement(1940..3481)
                     /gene="guaA"
                     /locus_tag="NBH32_002083"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019166941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCD1515662.1"
                     /translation="MEMAKEQELILVLDFGSQYNQLITRRIREMGVYSELHDHEISIE
                     EIKKMNPKGIILSGGPNSVYEEGSFTIDPEIYNLGIPVLGICYGMQLTTKLLGGKVER
                     ANEREYGKAIINAKSDELFVGLPAEQTVWMSHSDKVIEIPEGFEVIADSLSTDYAAIE
                     DKKRRIYGVQFHPEVRHTEYGNDLLNNFVRRVCDCKGQWTMENFIEIEIEKIRQRVGD
                     RRVLCAMSGGVDSSVVAVLLHKAIGDQLTCIFVDHGLLRKGEGDMVMEQFGEGFNMNI
                     IRVNAKDRFMNKLKGVSDPEQKRKIIGNEFVYVFDDEASKLKGVDFLAQGTLYTDVIE
                     SGTKTAQTIKSHHNVGGLPEDMEFELIEPINTLFKDEVRKLGIELGIPEHLVWRQPFP
                     GPGLGIRVLGEITEDKLEIVRESDAILRQVIREEGLEREIWQYFTVLPNIQSVGVMGD
                     YRTYDHTVGIRAVTSIDGMTSDFARIDWEVLQKISSRIVNEVDHVNRVVYDITSKPPS
                     TIEWE"
     gene            complement(3506..4972)
                     /gene="guaB"
                     /locus_tag="NBH32_002084"
     CDS             complement(3506..4972)
                     /gene="guaB"
                     /locus_tag="NBH32_002084"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002955225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCD1515663.1"
                     /translation="MWESKFAKESLTFDDVLLIPAQSDILPKDVDLSVQLSDKVKLNI
                     PVISAGMDTVTESKMAIAMARQGGLGVIHKNMGVEEQADEVQKVKRSENGVISNPFFL
                     TPEESVYEAEALMGKYRISGVPIVDNKEDRNLVGILTNRDLRFIEDFSIKIVDVMTQE
                     NLITAPVNTTLEEAEKILQKHKIEKLPLVKDGRLEGLITIKDIEKVIEFPNAAKDEHG
                     RLLVAAAIGISKDTDIRAQKLVEAGVDVLVIDTAHGHSKGVIDQVKHIKKTYPEITLV
                     AGNVATAEATKDLFEAGADIVKVGIGPGSICTTRVVAGVGVPQITAIYDCATEARKHG
                     KAIIADGGIKFSGDIIKALAAGGHAVMLGSLLAGTEESPGATEIFQGRQYKVYRGMGS
                     LGAMEKGSNDRYFQEDKAPKKFVPEGIEGRTAYKGALQDTIYQLMGGVRAGMGYTGSH
                     DLRELREEAQFTRMGPAGLAESHPHNIQITKESPNYSF"
     gene            complement(5010..6278)
                     /locus_tag="NBH32_002085"
     CDS             complement(5010..6278)
                     /locus_tag="NBH32_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000793018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HCD1515664.1"
                     /translation="MKNLILSVQHLLAMYAGAILVPIIVGTSLKFTPEQIAYLVTVDI
                     FMCGVATFLQANKVTGTGLPIVLGCTFTAVAPMILIGQTKGIDVLYGSLFLSGILVII
                     IAPFFSHLVKFFPPVVTGSVVTIIGINLMPVAMNYLAGGQGAKDYGDVKNILLGLMTL
                     IIILVLQRFTTGFIKSIAILIGLVLGTIGAGLLGMVDINQVNHAGWLGIPVPFRFSGF
                     SFDVTSTLVFFIVAIVSLIESTGVYHALSEITGKKLERKDFRKGYTAEGLAIVLGSIF
                     NSFPYTAYSQNVGLVSLSGAKKNNVIYGMVVLLLICGCIPKLGALANIIPLPVLGGAM
                     IAMFGMVMAYGVSILGHIDFKNQNNLLIIAVSVGLGTGISAVPQAFKGLGEQFAWLTQ
                     NGIVLGAISAIILNFFFNGIKYKQTEENVK"
     gene            complement(6278..6856)
                     /gene="xpt"
                     /locus_tag="NBH32_002086"
     CDS             complement(6278..6856)
                     /gene="xpt"
                     /locus_tag="NBH32_002086"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCD1515665.1"
                     /translation="MELLGQKVKEDGVVIDEKILKVDGFLNHQIDAKLMNEVGRTFYE
                     QFKDKGITKILTIEASGIAPAIMAALHFDVPCLFAKKAKPSTLTDGYYETSIHSFTKN
                     KTSTVIVSKEFLSEEDTVLIIDDFLANGDASLGLYDIAQQANAKTAGIGIVVEKSFQN
                     GHQRLEEAGLTVSSLCKVASLEGNKVTLVGEE"
     regulatory      complement(7087..7189)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            7369..7776
                     /locus_tag="NBH32_002087"
     CDS             7369..7776
                     /locus_tag="NBH32_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCD1515666.1"
                     /translation="MADITVVNDTGELYNVINQKKSEGYLESELTIISKSKLNLNDLH
                     DSEISLISTSGTFSDRMTKLLTGEDGEHAVLSRYNLAPDELEKYKQLILDDKMLVVAV
                     RDKSSHQEVHENNSAYEEIDITHFAEASKGPKA"
     gene            7919..8581
                     /locus_tag="NBH32_002088"
     CDS             7919..8581
                     /locus_tag="NBH32_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515667.1"
                     /translation="MEFKTIQSVKDPHYNEALKLYDTKLNLSLTEDKRIIKRSLENNK
                     TENDYVFIVGLENDVVVSLATAHYEATTNSAFLIYLFAKDLPNHDELMTQTLHQIEHE
                     LNLLSNLVHSRSINFLMMEVPKEPADLTQDNGLIIEHRRQFLYEHQFERQTEIDYIHP
                     TNDGHTKKVDLFLKKNIKLTKDIYGTSVKSNYILKYVFANGIAREIIYALLEEMDLRK
                     PF"
     gene            8699..9655
                     /locus_tag="NBH32_002089"
     CDS             8699..9655
                     /locus_tag="NBH32_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515668.1"
                     /translation="MLTKEFAQRVELSEKQVRKIVQHLEERGYQLSKTEYRGREATDF
                     KEEDIELFKDIADKVKQTNSYDLAFDELEKEKDFLQVIVKNDDKNLPTNQNVAQLVED
                     LRLEIQKMREERHLLGQMMNQVHQQQQELKELQNQLTSKIDSNSESLKAIQTSQEAIQ
                     EAQASQAKVLAESTNKVDAKSATDEKADSKDSKVAGVNTSTDAKTDTKADNAGDGTTT
                     KVDKEDQISATEAIEKASVEQSKNDNAAETSNKEATVDAEAQHDAEQQVAEAHAEASK
                     QATSNDSLEAKAENDSTASQSEMSEPKPQEEKKGFFARLFNL"
BASE COUNT         3358 a         1860 c         1546 g         3137 t
ORIGIN      
        1 taaacctttt catttttagt aagtttttat ttctccgaaa aattttatta aacgatatgc
       61 atgatttcaa tctagattga tttttcaatt acttatcgac ctttccataa actatgtcat
      121 tttcttacat atgtttcaat cttcaaccaa tctcaaagat agattggtcc tcgatgtttg
      181 aagtcgtgtt tcacgacgcc acatttacga ttaacatatc tagttataca aattcatctt
      241 ccactgtaac agtcgcgttt ctcaccgcat aataatctct aacaacctta atggatttaa
      301 ataaattaac gactcttaac agttaagaat aatattctga cacttatgca aaacaattaa
      361 atttaatagc aatctcttct aaactatttt atgaacggga tgacaatatg gtaatcaagt
      421 tcatgtgaaa aatttattag ttactggcta cacaactgga attttgaaaa tcatagggct
      481 aatgttattt gctgtagcaa ttttttcact aactatttat tttgcttata taatggaatc
      541 tttacttttt gggttcttac aaacagtcac ttcattagta cttttaatat gttcacttgt
      601 attagcttgg acttctttca tagttacatt acttttaata attgtgttaa gtggcatttt
      661 ctattttggt gcaaacgatt aattaaatta ttaaatgcta gttatacgaa ttaggacctt
      721 tcactcactt tgttttttaa tgattattta cttatatttc aaacatttgg ttttgcaaat
      781 tcactattaa aggagataaa tgtataatga aaatcacaaa aactattggt tcactagtct
      841 tgactgccgt tttattaacg tctgtttcac ctttaactaa cgcaaatgaa atacagacaa
      901 acaatacaaa aactgctcac caaagttatg aaaatataaa tggtgtgaaa attaatcgca
      961 atccaaactt gccaatgaat ccagaagcac aacaacgaat tggcggaaca aaagtagtta
     1021 aaactgctat aaagctaatt attaataata ggggcaaagc aaaaaatgtc atacaacaag
     1081 ttggcggcaa aaaagctgca actatttttg ataaagggta ttcaggtatt gtaaaagaat
     1141 tgaaaccatt gctaaaatgg tcagaagtac cagctaacgc tgtcggtgat gcagttaaac
     1201 gaggcgcaat aaatgccggt ctaagtaaag gtgatgctgg aataatctca actgtagtca
     1261 aagaagccat tgcatggtta ttctaaaatg ataaaggagt gtttcatatg cttgaagata
     1321 acggcgatat cagattgcta tatcaatcaa tagaagaact tgcttcagtt ttgggtcaaa
     1381 gtcaattcga aactaaaact gtcagcttaa tatttgtaca aatggactta agatatagtg
     1441 ttttcgaaaa agtgcaaact gattttatga agtatttagc acataaaaat tctgatgaaa
     1501 taaaatatat agacttaatc aaaataatag aaaatgcatt acctgaagat aaagaactta
     1561 gtatcaatgt gattaattcc attattattg gtttcgctaa taactattac ccaatattgc
     1621 aaagtttagt accggaaata caaagtagtt atggcataac cataaataaa aatttcaata
     1681 tagatagtat agatttttaa atactattta ataagaatag gaaaccgatc ccaggacata
     1741 aaatccaaat cgtaggctct acaatcttat attggtagta gttgactgcg gagccccaac
     1801 ataaagaaat tggtacgcca atttctacag atataactcc catcatttca tcacatgatg
     1861 ttgcccatac caatcgcacc aaaaaaggct agaaaccgcg taataacggt acttctagcc
     1921 tatcatttgc tatatataat tattcccact caattgtgct tggtggtttt gatgtaatgt
     1981 catagactac gcggttgacg tggtctactt cgtttacgat acgacttgaa atcttttgta
     2041 agacttccca atcgatgcgt gcaaaatcac ttgtcatgcc gtcgatagat gttactgcac
     2101 gaatacctac tgtgtgatca tacgtacggt agtctcccat aacacctact gattgaatgt
     2161 ttggtaacac tgtgaagtat tgccaaattt ctctttcaag accttcttct ctaatcactt
     2221 ggcgtaaaat cgcgtctgat tctctaacga tttctagttt atcttcagta atttctccaa
     2281 gtacacgaat accaagacca ggtcctggga atggttgtct ccacactaaa tgttctggaa
     2341 tacctagttc aatacctaat ttacgtactt catctttaaa caatgtattg attggttcga
     2401 ttaattcgaa ttccatgtct tctggtaatc caccaacatt gtggtgtgat ttgattgttt
     2461 gtgctgtctt agtacctgat tcgatgacgt ctgtatatag tgttccttgc gcaaggaagt
     2521 ctacaccttt gagttttgat gcttcatcat caaatacata tacaaattca ttaccaatga
     2581 ttttacgttt ttgttcagga tctgaaacac cttttaattt attcataaaa cgatctttcg
     2641 cattaacacg aataatattc atgttgaacc cttcaccgaa ttgttccata accatgtcgc
     2701 cttcaccttt acgaagtaag ccatggtcta caaagataca tgttagttga tcaccaattg
     2761 ctttatgcaa tagtacagct acaacagatg aatctacgcc gccactcatc gcacataata
     2821 cacgacggtc tcctacgcgt tgacgaatct tttcaatttc gatttcgata aagttttcca
     2881 ttgtccattg acctttacaa tcacaaacac gacggacaaa attatttaat aaatcattac
     2941 catattctgt atgacgtact tctggatgga attgaacacc ataaatgcga cgtttcttat
     3001 cttcgattgc tgcatagtct gtgcttaggc tatcagcgat aacttcaaag ccttctggaa
     3061 tttcaataac tttatcagaa tgactcatcc aaacagtttg ttctgctggt aagccaacga
     3121 ataactcatc tgacttcgca ttaatgattg ctttaccgta ttcacgttca ttggcacgtt
     3181 caactttacc acctaataat ttagtagtta attgcatgcc gtaacaaata ccaagtactg
     3241 gaattcctaa attatatatt tccggatcaa ttgtaaatga accttcttca taaactgaat
     3301 ttggaccacc tgataagatg ataccttttg gattcatttt cttaatttct tcaattgaaa
     3361 tttcatgatc gtgtaattca ctataaacgc ccatttcacg aattcggcgt gtaattaatt
     3421 ggttgtattg gctaccaaag tctaagacaa ggattaactc ttgttctttt gccatttcca
     3481 tatttgtcgt tctcctttat cttaattaga atgagtagtt cggtgattct ttcgtaattt
     3541 gaatattatg tggatggctt tctgctaaac cagcaggacc catacgtgta aattgtgctt
     3601 cttcgcgtaa ttctcttaaa tcgtgtgaac cagtataacc cataccagca cgcacaccgc
     3661 ccattaattg gtaaattgta tcttgtaacg cacctttata agccgtacgt ccttcgatac
     3721 cttcaggaac aaatttctta ggtgctttgt cctcttggaa gtaacgatcg tttgaacctt
     3781 tttccatcgc acctaaagag cccataccac ggtatacttt atattgtcta ccttggaaaa
     3841 tttctgttgc gcctggactt tcttcagtac ctgctaataa gctacctaac ataaccgcat
     3901 gtccaccagc agctaatgct ttaatgatat ctcctgagaa tttaatacca ccatcagcaa
     3961 tgatagcttt accatgtttg cgtgcttcag ttgcacaatc ataaattgct gtaatttgtg
     4021 gtacaccaac acctgctaca acacgcgtcg tacaaattga acctgggcca ataccaactt
     4081 taacaatatc tgcacccgct tcaaataaat cttttgttgc ttctgcagtt gctacgttac
     4141 ctgctactaa tgtgatttct gggtaagtct tcttaatatg tttcacttga tcgataacac
     4201 ctttagagtg accatgtgct gtatcgataa ctaagacatc cacacctgct tcgactaatt
     4261 tttgagcacg aatatcagta tcttttgaaa taccaattgc tgcggctaca agtagacgac
     4321 catgttcatc ttttgctgca ttagggaatt cgataacttt ttcaatatct ttaatagtaa
     4381 taagaccttc tagacgtccg tctttaacta atggtaactt ttcaatttta tgtttctgga
     4441 gaattttttc tgcttcttca agtgttgtat tcactggagc tgtaattaaa ttttcttgcg
     4501 tcattacatc tacaatttta atcgagaagt cttcaataaa acgtaagtca cggtttgtta
     4561 aaatacctac taagttgcga tcttctttat tatcaacaat tggtacacct gaaatacggt
     4621 atttacccat taatgcttcc gcttcataaa cactttcttc tggtgttaag aaaaatgggt
     4681 ttgaaataac accattttct gagcgtttta ctttttgaac ttcgtccgct tgttcttcaa
     4741 cgcccatatt tttatgaata acacctaaac caccttgacg agccatagca atcgccattt
     4801 tagattcagt tactgtatcc ataccagcag aaataactgg aatattcaat ttaactttgt
     4861 ctgataattg tacgcttaaa tcaacgtctt tcggtaaaat atcagattgt gctggaatta
     4921 ataacacatc atcaaacgtt aatgattctt ttgcaaattt actttcccac attaaaaaca
     4981 gcctccattt ttcaaattaa ttagttatat tatttcacat tttcttccgt ttgtttatac
     5041 tttattccat taaaaaagaa attaagaata attgcagaga ttgcgcctaa aacaattccg
     5101 ttttgagtca accatgcaaa ttgttcacct aaacctttaa atgcttgtgg tacagcgctt
     5161 ataccagtac ctaatcctac tgatacagcg ataattaata aattgttttg atttttaaaa
     5221 tcgatatgtc ctaatatact aacaccatat gccattacca tgccaaacat agctatcatc
     5281 gcaccaccta acacaggtag cggtatgata tttgctaatg caccaagctt aggtatacaa
     5341 ccacatataa gcaataacac gaccatgccg tatataacat tgtttttctt agcgccggat
     5401 aaagaaacaa gtcctacatt ttgcgaatag gctgtatacg gaaatgaatt gaatatagaa
     5461 cctaacacta tcgctagacc ttccgcagta taacctttac gaaaatcttt tctttctaac
     5521 ttcttaccgg taatttcact taacgcatga tagacacctg tcgactcaat taaactaacg
     5581 atagctacaa taaagaacac taacgtcgat gtcacatcaa agctaaatcc tgagaatcta
     5641 aacggcactg ggatgcctaa ccaaccggca tgattgactt gattaatatc gaccatccca
     5701 agtaagccag cgcctatcgt tcctaaaacg agtccaatta atatggcaat actcttaata
     5761 aatccagttg tgaatctttg taaaacaaga ataatgatta atgtcattaa acctaacaaa
     5821 atgttcttaa catctccata gtcctttgca ccttgacctc cagctaagta attcattgct
     5881 actggcatta aattgatacc aatgatagta acaacactac ccgttactac tggtgggaag
     5941 aattttacaa gatgtgaaaa gaaaggcgcg atgataataa ctaatatccc tgataaaaat
     6001 agcgaaccat aaagtacatc tattcctttc gtttgaccaa ttaaaatcat gggcgcaaca
     6061 gccgtgaatg tacatccaag aacgattggt aatcctgttc ctgttacttt attggcttgt
     6121 aaaaatgtgg caaccccaca cataaatata tctactgtaa ctaagtaagc gatttgttca
     6181 ggtgtaaact tcaaacttgt accaacaatg attggaacta agatggcacc tgcgtacata
     6241 gctaaaagat gttgaacact taggattaaa tttttcatta ttcttctccc accaatgtca
     6301 ctttgtttcc ttctagtgaa gcaaccttgc agagagaaga aactgttaaa cctgcttctt
     6361 ctaaacgttg atgcccattt tggaaactct tttcaacaac aataccaata ccagctgtct
     6421 tagcattcgc ttgctgtgcg atatcgtata atcctaatga agcatcacca tttgctaaaa
     6481 agtcatcgat gatgagtaca gtatcttctt ctgataaaaa ttcttttgaa acaatgaccg
     6541 tacttgtttt atttttagta aatgaatgaa tagatgtttc ataataacca tccgtcaaag
     6601 tgctaggttt tgcttttttc gcaaataaac atggcacatc aaaatgcagt gcagccatga
     6661 ttgcaggtgc gataccggaa gcttcaatgg ttaagatttt agtaatccct ttatctttaa
     6721 attgctcgta aaaagtgcga ccaacttcat tcattaactt tgcatcaatt tgatgattta
     6781 aaaatccatc gacttttaaa atcttctcat caatgacaac gccgtcttcc tttacttttt
     6841 gtcctagtaa ctccactctt aaaacctcct cagatttgtg tgaaacattt tgctcttaaa
     6901 ttggtgctag atacaaaaaa atccccaaac taaataatag tttcagggtt tatgagtgaa
     6961 cgaacatgca taacgaattt gtcatgcaat caatgtaaaa gaagtttcat caaataaact
     7021 gtgaccatca tataaaatga tataaatcac ccaccatggt tacaatttaa tggctgaagc
     7081 tactcctagt attgtgttgt tactcatagt catgtcgttc aaggcaacat ggtagaaact
     7141 tctaaagcca tattctttag attatatgag tttatgtaaa ttatttaacg ataatagcaa
     7201 attttcggca ttttttcaat aactgcttag gtaatctttt aatagtttta taaatgtaca
     7261 gagacgttta atgcgaatga agacttataa cttaataatg taagggtttt actttataat
     7321 aagaagggta aaagattaaa taaacaaatt tttaaagggg cgacaactat ggcagatatt
     7381 actgtagtaa atgacactgg cgaattatac aacgtaatca atcaaaagaa atctgaaggt
     7441 tatttagaat cagagctaac aattattagt aaaagtaaat taaacctaaa tgacttacac
     7501 gactctgaaa tctcccttat ctcaacaagt ggaacattta gtgatagaat gactaaactg
     7561 ttaacaggag aagatggtga acatgcagtt ttatcacgct acaacttagc gcctgatgaa
     7621 ttagaaaaat ataaacaact tattttagac gataaaatgt tagttgtagc ggttcgtgac
     7681 aaatcatcac atcaagaagt gcatgaaaat aattcagctt atgaagagat tgatattaca
     7741 cattttgctg aagcatctaa aggtcccaaa gcttagttat cacactgaat tcaaaatgag
     7801 attactgtac tgtctaattt aagatagtac agttttttat accatttaaa agtttatgaa
     7861 cagtctttta cagcaggcga ttatgctata atattattta aattggggag gtgttcttat
     7921 ggaatttaaa accattcaat ctgtcaaaga tccacattac aacgaagcac ttaaacttta
     7981 cgatactaag ttaaatttat ctttaactga agataaacgt atcatcaaac gctcattaga
     8041 aaacaacaaa accgaaaacg attatgtttt tatcgtaggc ttagaaaacg atgttgttgt
     8101 cagtctagca actgcacatt atgaagccac aaccaattca gcatttttaa tttatttatt
     8161 cgcaaaagac ctacccaacc atgatgagtt aatgacgcaa acattacatc aaattgaaca
     8221 tgaactaaac ttattatcaa accttgttca ttctagaagt attaactttt taatgatgga
     8281 agtccctaaa gaacctgcag atttaacaca agataacggc ctcattattg aacaccgtag
     8341 acagttttta tacgaacatc aatttgaaag acaaactgaa attgattaca ttcatccaac
     8401 taatgatggt cacactaaaa aagtggactt atttttaaag aaaaatatta aactgacaaa
     8461 ggatatctac ggtacaagtg ttaaatcaaa ttatattttg aagtatgtat ttgccaatgg
     8521 tatcgcacgt gaaattattt atgctttatt agaagaaatg gatttacgta aaccatttta
     8581 aacaaacttg aaatcactaa caataacgtt taaactatgt atgaagcaag actgacgttt
     8641 cactgcccgt agacaaacgt cagtatacat aaagtatatg cagaaaggac tttaaagtat
     8701 gttaactaaa gaatttgcac aacgcgtaga actaagtgag aagcaagtcc gtaaaattgt
     8761 tcaacattta gaagaacgtg gttaccaact aagcaagact gaatatcgtg gtcgtgaagc
     8821 aactgatttc aaagaagaag atatcgagct tttcaaagac attgccgaca aagtaaaaca
     8881 aacaaatagt tatgatctag cgtttgatga attagaaaaa gaaaaagact tcctgcaagt
     8941 cattgtcaaa aacgatgaca aaaacttacc tactaatcaa aatgtcgctc aattagtaga
     9001 agatttacgc ctagaaatcc agaaaatgcg cgaagaacgt cacctacttg gtcaaatgat
     9061 gaatcaagta catcagcaac aacaagaatt aaaagaactt caaaatcaac ttacatctaa
     9121 aatcgattca aatagcgaat ccttaaaagc catccaaaca tcacaagagg ctatccaaga
     9181 agcacaagca tctcaagcta aagtattggc tgaatcaaca aataaagttg atgctaagtc
     9241 cgctactgat gaaaaggctg acagtaaaga ttcgaaggtt gctggagtga atacatcaac
     9301 agacgctaag acggatacta aagcagacaa tgcaggcgat ggcactacta ctaaagtaga
     9361 taaagaggat caaatttctg caactgaagc aattgaaaaa gccagcgttg agcagtctaa
     9421 aaatgacaat gcagctgaga cttcaaataa agaagctaca gtagatgcag aagcgcaaca
     9481 tgatgctgaa caacaagtag cagaagccca tgcagaagct tctaaacaag ctacttcgaa
     9541 cgattcattg gaagcgaaag cagaaaatga cagcacagct tcacagtctg aaatgtcaga
     9601 acctaaacct caagaagaga aaaaaggttt cttcgcacgt ttattcaacc tgtagggagt
     9661 gggacagaaa tgataaagag ccactaataa tttattatgt agtggttctt acacattagc
     9721 cacagctaat gtgtacttaa aaataggaat acatgagtaa aactcatgca taagaaatac
     9781 taatttctat agaaaaagta tttctttatc gtcgtcccac cccaacttgc attgcctgta
     9841 gaatttcttt tcgaaattct ctgtgttggg gcccctgact agaatttaaa aaagcttgtt
     9901 a
//
LOCUS       DAHHOJ010000097         9807 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.097, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000097 DAHHOJ010000000
VERSION     DAHHOJ010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9807
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.097"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(29..415)
                     /locus_tag="NBH32_002090"
     CDS             complement(29..415)
                     /locus_tag="NBH32_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1515669.1"
                     /translation="MSASLYIAIILVIAIIAYMIVQQILNKRAVKELDQNEFHNGIRK
                     AQVIDVREKVDYDYGHINGSRNIPMTMFRQRFQGLRKDQPVYLCDANGIASYRAARIL
                     KKNGYTDIYMLKGGYKKWTGKIKSKK"
     gene            573..1403
                     /locus_tag="NBH32_002091"
     CDS             573..1403
                     /locus_tag="NBH32_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD1515670.1"
                     /translation="MTETWNFINTGSKDPYYNMAMDEALLNFVSRGEIDPVIRFYTWN
                     PATLSIGYFQRLQKEIDIDKVKEKGFGLVRRQTGGRGVLHDKELTYSVIVPESHPNMP
                     STVTEAYRVISQGLLEGFKNLGFDTYFAVPKTPEERQKLKQPRSSVCFDAPSWYELVV
                     EGRKIAGSAQTRQKGVILQHGSILQDIDIDELFDMFIYKNERLKLKMKEAFVEKAVAI
                     NDISDKHITISQMEEAFEKGFKKGLNIELKPLELTEAQLAEVEELTEKYRSDEWMFRK
                     "
     gene            complement(1467..1685)
                     /locus_tag="NBH32_002092"
     CDS             complement(1467..1685)
                     /locus_tag="NBH32_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515671.1"
                     /translation="MRNIIFYLVLIIAAIGLVMNLDAFIFSIVRMLVSFAVIAGIIYL
                     IYYFFILTEDQRKYRKAMRKYKRNQRRK"
     gene            complement(1699..2280)
                     /locus_tag="NBH32_002093"
     CDS             complement(1699..2280)
                     /locus_tag="NBH32_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515672.1"
                     /translation="MKKLVSIVGATLLLAGCGSQNLAPLEEKTTDLREDNHQLKLDIQ
                     ELNQQISDSKSKIKGLEKDKENSKKTASNNTKIKLMNVTSTYYDKVAKALKSYNDIEK
                     DVSKNKGDKNVQSKLNQISNDIQSAHTSYKDAIDGLSLSDDDKKTSKNIDKLNSDLNH
                     AFDDIKNGYQNKDKKQLTKGQQALSKLNLNAKS"
     gene            2385..3446
                     /locus_tag="NBH32_002094"
     CDS             2385..3446
                     /locus_tag="NBH32_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HCD1515673.1"
                     /translation="MSRITQVHRILEQKHLDAIIILSDYNRRYLSGFTGTSGALIISK
                     DKQYLITDFRYIDQATKQAPNYEIINRKSTIIGEIKELLHQENFENVGFEGHHVSYDT
                     YLELNKSRISLISISNTVDKIRDVKDADEIALIQKAANIVDETYEYILTVVKAGMTEK
                     ELKAILESKMLELGADGPSFDTIVASGHRGALPHGVASDKIIEKGDMITLDFGAYYNG
                     YCSDITRTFAIGEPDPKLKEIYQIVLESQMKAINEIRPGMTGAEADAISRNYLESKGY
                     GKEFGHSLGHGIGLEIHEGPMLARTIQDKLQVNNCVTVEPGVYIEGLGGIRIEDDILI
                     TENGCQVFTKCTKDLIVLT"
     gene            3472..4029
                     /gene="efp"
                     /locus_tag="NBH32_002095"
     CDS             3472..4029
                     /gene="efp"
                     /locus_tag="NBH32_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000626504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCD1515674.1"
                     /translation="MISVNDFKTGLTISVDNAIWKVIDFQHVKPGKGSAFVRSKLRNL
                     RTGAIQEKTFRAGEKVEPAMIENRRMQYLYADGDNHVFMDNESFEQTELSSDYLKEEL
                     NYLKEGMEVQIQTYEGETIGVELPKTVELTVTETEPGIKGDTATGATKSATVETGYTL
                     NVPLFVNEGDVLIINTGDGSYISRG"
     gene            4503..4967
                     /gene="accB"
                     /locus_tag="NBH32_002096"
     CDS             4503..4967
                     /gene="accB"
                     /locus_tag="NBH32_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCD1515675.1"
                     /translation="MNFKEIKELIEILDKSTLTEINIEDTKGKVTLKKEKETEIITPQ
                     ISQMPVEAAAMPMPQVQSTDSNKTEAPKPTSDNHKTINAPMVGTFYKSPSPDEEAYVQ
                     VGDTVSNETTVCILEAMKLFNEIQAEISGEIVEILVEDGQMVEYGQPLFKVK"
     gene            4967..6322
                     /gene="accC"
                     /locus_tag="NBH32_002097"
     CDS             4967..6322
                     /gene="accC"
                     /locus_tag="NBH32_002097"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCD1515676.1"
                     /translation="MKKVLIANRGEIAVRIIRACRDLGIQTVAIYSEGDKDALHTQIA
                     DEAYCVGPTLSKDSYLNIPNILSIATSTGCDGVHPGYGFLAENADFAELCEACQLKFI
                     GPSYQSIQKMGIKDVAKAEMIKANVPVVPGSDGLMKDVSEAKKIAQKIGYPVIIKATA
                     GGGGKGIRVARDEKELETGFRMTEQEAQTAFGNGGLYMEKFIENFRHIEIQIVGDSYG
                     NVIHLGERDCTIQRRMQKLVEEAPSPILDDETRREMGNAAVRAAKAVNYENAGTIEFI
                     YDLNDNKFYFMEMNTRIQVEHPVTEMVTGIDLVKLQLQVAMGEVLPYKQEDIKLTGHA
                     IEFRINAENPYKNFMPSPGKIEQYLAPGGYGVRIESACYTNYTIPPYYDSMVAKLIIH
                     EPTRDEAIMAGIRALSEFVVLGIDTTIPFHIKLLNNDIFRSGKFNTNFLEQNSIMNDE
                     G"
     gene            6337..6699
                     /locus_tag="NBH32_002098"
     CDS             6337..6699
                     /locus_tag="NBH32_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HCD1515677.1"
                     /translation="MVKVTDYSNSKLGKVEIAPEVLSVIASIATSEVEGITGHFAELK
                     ETNLEKVSRKNLSRDLKIESKEDGIYIDVYCALKHGVNISKTANKIQTSIFNSISNMT
                     AIEPKQINIHITQIVIEK"
     gene            6759..7148
                     /gene="nusB"
                     /locus_tag="NBH32_002099"
     CDS             6759..7148
                     /gene="nusB"
                     /locus_tag="NBH32_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCD1515678.1"
                     /translation="MSRKESRVQAFQTLFQLEMKDSDLTINEAISFIKDDNPDLDFEF
                     IHWLVSGVKDHEPVLDETISPYLKDWTIARLLKTDRIILRMATYEILHSDTPAKVVMN
                     EAVELTKQFSDDDHYKFINGVLSNIKK"
     gene            7165..8502
                     /locus_tag="NBH32_002100"
     CDS             7165..8502
                     /locus_tag="NBH32_002100"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCD1515679.1"
                     /translation="MSDYLSVSALTKYIKYKFDQDPHLQSVLIKGELSNFKKHSSGHL
                     YFNVKDKESVISAMMFKGSASKLNFEPKEGDEVLLEARVSVFERRGNYQIYVNKMQLD
                     GIGNLYQKLEALKKKLTEEGCFDKANKKSIPKFPKKIAVLTASTGAAIRDIHSTINSR
                     FPLAEQIQISTLVQGEKAKDDIIEKIEYADSLGVDTIIVGRGGGSIEDLWNFNEEAVV
                     RAIYNCKTPIISAVGHETDFTLSDFAADIRAATPTQAAVIATPDQYELLQQIQQYQFT
                     LTRFIKKHLEQQRKHVEHLSSYYKFKQPTLLYDQQIQRRDDLEKRLKQQIQATFEQQR
                     HRLMLLQQRYNLKALLSSVNQEQQNNLQLTNQLVKLLNSKILSYKNDLKNKVENLNNL
                     SPTNTMLRGYAIVNKKDEVITSTKDLTENDQLTLTMKDGLVDAKVTKVRCNND"
     gene            8495..8725
                     /locus_tag="NBH32_002101"
     CDS             8495..8725
                     /locus_tag="NBH32_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCD1515680.1"
                     /translation="MTKETQSFEEMMQELEQIVQKLDNETVSLEESLDLYQRGMKLSA
                     ACDTTLKNAEKKVNDLIKEEAEDVKNDESTDE"
     gene            8703..9584
                     /locus_tag="NBH32_002102"
     CDS             8703..9584
                     /locus_tag="NBH32_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1515681.1"
                     /translation="MTNLPMNKLIDEVNNELSVAINKSVMDTQLEESMLYSLNAGGKR
                     IRPVLLLLTLDSLNTEYELGMKSAIALEMIHTYSLIHDDLPAMDNDDYRRGKLTNHKV
                     YGEWTAILAGDALLTKAFELISSDDRLTDEVKIKVLQRLSIASGHVGMVGGQMLDMQS
                     EGQPIDLETLEMIHKTKTGALLTFAVMSAADIANVDDATKEHLESYSYHLGMMFQIKD
                     DLLDCYGDEAKLGKKVGSDLENNKSTYVSLLGKDGAEDKLTYHRDAAVYELTQIDEQF
                     NTKHLLEIVDLFYSRDH"
BASE COUNT         3622 a         1359 c         1772 g         3054 t
ORIGIN      
        1 attaaatcgt atattaaatt tacaaaaact attttttaga ctttattttt ccagtccatt
       61 ttttatagcc gccttttaac atatagatat ctgtatatcc attctttttc aaaatacgag
      121 cggctctata gctagcaatc ccattggcat cacataagta taccggttgg tcttttctta
      181 atccttggaa tcgttgcctg aacattgtca taggaatatt gcgagaccca ttaatgtgac
      241 cgtagtcata gtcaactttc tctctaacat cgatgacttg agcttttcta atcccattat
      301 ggaattcatt ttgatctaat tctttaacag ctcgcttgtt aagaatttgt tgaacaatca
      361 tataagcaat aattgctata actaaaatta ttgcgatgta caaactagca ctcatcttgc
      421 atcctcccta attaaacgat aatataatta taagactgtt aggtcaaatt atcaaaatat
      481 ttcactaaag aattgcaatt attattcatt tagttaaatt gattaattag cgaatcaaat
      541 attatacatt ttgctgatag gagttcttga atatgactga aacttggaat tttattaata
      601 caggaagtaa agacccatat tataatatgg caatggatga agcgttatta aattttgtct
      661 ctagaggtga aattgatcca gttatacgtt tttacacatg gaatcctgca acattatcaa
      721 tagggtattt ccaaaggtta caaaaggaaa ttgatataga taaggttaaa gagaaaggtt
      781 ttggtctcgt aaggcgtcaa actggtggcc gtggggtgct acatgataaa gagttaactt
      841 acagtgtgat tgtgcctgaa tcgcatccga atatgccttc tacagttaca gaagcgtaca
      901 gagtgatttc acaaggatta ttagaaggat ttaaaaactt aggatttgat acttattttg
      961 cggtacctaa aacgcctgaa gagcgtcaaa aattaaaaca acctcgaagt tcagtgtgtt
     1021 ttgatgcacc aagttggtat gagctagttg ttgaaggccg taagattgct gggagtgctc
     1081 aaacgagaca aaagggcgta atcctccaac acggttcgat tttacaagat attgatattg
     1141 atgaactgtt tgatatgttt atttataaaa atgaaagatt aaaattaaaa atgaaagagg
     1201 cattcgttga aaaagcagtt gccatcaatg atatttcaga taaacacatt actattagtc
     1261 aaatggaaga agctttcgag aaaggtttta agaaaggttt aaatatagaa ttaaagccac
     1321 ttgaattaac agaagctcaa ttggcagaag tggaagaatt aactgaaaaa tatcgttcag
     1381 atgaatggat gtttagaaag tagtttttag aatgaaaaat ggaagcacgt agactatttt
     1441 acctacaagt gcttccattt ttttatctat tttcttcttt gatttctttt atacttacgc
     1501 attgctttac ggtatttacg ttgatcttca gttaaaatga agaaataata tataagataa
     1561 ataataccag ctattacagc aaagctgact aacattctga cgattgaaaa aataaaggca
     1621 tctagattca ttactaatcc aatcgcagca ataataagta caagataaaa tattatatta
     1681 cgcattaaca gactcctatc atgattttgc atttaagttt aattttgaca acgcttgttg
     1741 tccttttgta agttgttttt tatctttatt ttgatagcca tttttaatat catcaaatgc
     1801 atgattcaaa tcagagttta atttatcgat atttttagac gtttttttat catcatcact
     1861 aagtgataaa ccatcgatag catctttgta tgaagtgtga gcactttgaa tatcattaga
     1921 aatttgattt aatttcgatt gaacattctt atcgcctttg tttttactta catctttctc
     1981 aatatcgtta taggatttca aagctttagc aactttgtcg tagtatgttg atgtaacatt
     2041 catcaattta attttcgtat tattagatgc agttttttta ctgttttctt tatccttttc
     2101 aagcccttta attttagatt tagaatcact aatttgttga ttaagttctt gaatatctag
     2161 tttgagttga tgattatctt ctcttaaatc tgttgttttt tcttctaatg gtgctaaatt
     2221 ttgtgatcca catccagcta acaataatgt tgcgccaaca attgaaacca attttttcat
     2281 tactatctcc ctaaaatcaa tatctattat tttttatcct tattatgata ttattttaat
     2341 gtataaagtt gaaatgcaca aacgtttcac taggaggtca taagatgagc agaataacac
     2401 aagtacatcg tattttagaa caaaaacatt tagatgcaat aatcatactt tcagattaca
     2461 atagaagata tttgtcagga tttactggga ctagcggtgc gctaatcata tctaaagata
     2521 aacaatattt gataacagac tttagatata ttgatcaagc aactaagcag gcgccaaatt
     2581 atgaaattat taatcgtaaa tctactatta ttggtgagat taaagaattg ctacaccaag
     2641 aaaattttga aaatgtaggt tttgaggggc accatgtaag ttatgataca taccttgaat
     2701 taaataaaag ccgtatatca ttaataagca tttctaatac tgtagataaa attagagacg
     2761 tcaaagatgc tgacgaaatt gctttaattc aaaaagcagc taatattgtt gatgaaacat
     2821 atgaatatat tttaactgtt gtaaaagcag gtatgactga aaaagaatta aaggcaatat
     2881 tagaaagtaa aatgctagaa ttaggagcag atggaccatc attcgatacg attgtagcat
     2941 ctggtcatag aggtgcatta ccacatggtg ttgcaagtga taaaattatt gaaaaaggcg
     3001 acatgattac attagatttt ggcgcgtatt ataacggcta ttgttcagat attactagaa
     3061 catttgctat tggagaacca gatcctaaac tgaaagaaat atatcaaata gtacttgaat
     3121 ctcaaatgaa agcaattaat gagattagac ctggcatgac tggtgcagaa gctgatgcca
     3181 tttcaagaaa ctatttagag tcaaaagggt atggaaaaga atttggacat tcactaggac
     3241 atggtattgg tttagaaatc catgaagggc caatgctggc tcgtacgata caagataaac
     3301 ttcaagttaa caactgtgtt acagtagaac ctggtgttta tatagaaggt ttgggcggta
     3361 taagaataga agatgatatt ttaattacag aaaatggttg tcaagtcttt actaaatgca
     3421 caaaagacct tatagtttta acataagcgt gtaaaatgag gaggaaactg aatgatttcg
     3481 gttaatgatt ttaaaacagg tttaacaatt tctgttgata acgctatttg gaaagttata
     3541 gacttccaac atgtaaagcc tggtaaaggt tcagcattcg ttcgttcaaa attacgtaat
     3601 ttaagaactg gtgcaattca agagaaaacg tttagagctg gtgaaaaagt tgaaccagca
     3661 atgattgaaa atcgtcgcat gcaatattta tatgctgacg gagataatca tgtatttatg
     3721 gataatgaaa gctttgaaca aacagaactt tcaagtgatt acttaaaaga agaattgaat
     3781 tacttaaaag aaggtatgga agtacaaatt caaacatacg aaggtgaaac tatcggtgtt
     3841 gaattaccta aaactgttga attaacagta actgaaacag aacctggtat taaaggtgat
     3901 actgcaactg gtgctactaa atcggcaact gttgaaacag gttatacatt aaatgtacct
     3961 ttatttgtaa acgaaggtga cgttttaatt atcaacactg gtgatggaag ctacatttca
     4021 agaggataat ctctaatttg ttaacaaata gcttgtatac actatactga tttaacgtaa
     4081 gatattccaa ataagtctca taaagctatt gcctaaaatg attataggtt atatgctgat
     4141 atgaggcttt ttatttttaa aataattttt aaaatataaa acgaatcgtc taattggtag
     4201 tgaaaaaaca tagacatgag taaaggtata tatctacata tcatattgtt gaggattaaa
     4261 cataatagct aataaaaata acattgatat gtttaactga atgcataaaa tttttaatca
     4321 agagttaaga tgagtcaatg ttaagtaata aatacacaag tgtttttaat ttaaaatgtg
     4381 tttttattat aactttggga tagacttata atgtcgccag caatatgaac atgcttgaat
     4441 tgaagagttg tctcaagtaa aatagacggg tagatgaaaa caaactgaag gagtcagtaa
     4501 taatgaactt taaagaaatc aaagaattaa ttgaaattct ggataaatca actttaacgg
     4561 aaatcaatat tgaagatact aaaggcaaag tgacgcttaa gaaagaaaaa gaaactgaga
     4621 ttatcacgcc acaaatttca caaatgccag ttgaagctgc ggcaatgcct atgcctcaag
     4681 tacaatcaac tgatagcaat aaaactgaag ctccaaagcc aacttcagat aatcacaaaa
     4741 caattaatgc acctatggta ggtacatttt acaaatcgcc ttctccagac gaagaagcat
     4801 atgtgcaagt tggggacact gtttcaaatg aaacaacagt gtgtatttta gaggcaatga
     4861 aactatttaa tgaaattcaa gcagaaattt caggtgaaat tgttgaaatc ttagtagaag
     4921 acggacaaat ggtagagtat ggccaaccgt tatttaaggt gaaataatga aaaaggtttt
     4981 aattgcaaac cgcggtgaaa tcgcagttag gattattcgc gcttgtcgtg atttaggcat
     5041 ccaaactgtt gcaatctatt ctgaagggga taaagatgcg ctacatactc aaattgctga
     5101 tgaagcatat tgcgtaggtc ccactttgtc taaagattca tatttaaata ttccgaacat
     5161 cttatctatt gcaacttcta caggttgtga tggcgttcat ccgggttatg gctttttagc
     5221 tgaaaatgct gactttgcag aattatgcga agcatgccaa ttgaagttca ttggaccaag
     5281 ttatcaatct atccaaaaaa tgggtatcaa agatgttgct aaggcagaaa tgatcaaagc
     5341 caatgttcca gttgttcctg gtagtgacgg tttaatgaaa gacgtctcag aagctaagaa
     5401 aatcgcccaa aaaattggct atccggtcat cattaaagct actgctggcg gtggcggaaa
     5461 aggtatccgt gttgctcgtg atgaaaaaga acttgaaact ggcttccgaa tgacagaaca
     5521 agaagctcaa actgcatttg gtaatggtgg actttatatg gagaaattca tcgaaaactt
     5581 ccgccatatt gaaatccaaa ttgttgggga cagctatggt aatgtaattc atttaggaga
     5641 acgtgattgt acaattcaaa gacgtatgca gaaattagtg gaagaagcac cttccccaat
     5701 tttagatgat gaaacacgtc gtgaaatggg aaatgccgca gtgcgtgcag cgaaagctgt
     5761 aaattatgaa aatgcgggaa caattgagtt tatatatgat ttaaatgata ataaatttta
     5821 ttttatggaa atgaatacac gtattcaagt agaacatcct gtaactgaaa tggtaacagg
     5881 aattgattta gttaaattac aattacaagt tgctatgggt gaagtgttac catataaaca
     5941 agaagatatt aaattaacag gacacgcaat tgaatttaga attaatgctg aaaatcctta
     6001 caagaacttt atgccatcgc caggtaaaat tgagcaatat cttgcaccag gtggatatgg
     6061 tgttcgaata gagtcagcat gttatactaa ttatacgata ccgccatatt atgattcgat
     6121 ggtagcgaaa ttaatcatac atgaaccgac acgagatgaa gcgattatgg ctggcattcg
     6181 tgcattaagt gaatttgtgg ttcttggtat tgatacaact attccattcc atattaaatt
     6241 attgaataac gatatattta gaagcggtaa atttaataca aactttttag agcaaaatag
     6301 cattatgaat gatgaaggtt aataggaggt caatccatgg tcaaagtaac tgattattca
     6361 aattcaaaat taggtaaagt agaaatagcg ccagaagtgc tatctgttat tgcaagtata
     6421 gctacttcgg aagtcgaagg catcactggc cattttgctg aattaaaaga aacaaattta
     6481 gaaaaagtta gtcgtaaaaa tttaagccgt gatttaaaaa tcgagagtaa agaagatggc
     6541 atatatatag atgtatattg tgcattaaaa catggtgtta atatttcaaa aactgcaaac
     6601 aaaattcaaa cgtcaatttt taattcaatt tctaatatga cagcgataga acctaagcaa
     6661 attaatattc acattacaca aatcgttatt gaaaagtaat gtcataccta attcagtaat
     6721 taaataaaga aaaatacaaa cgtttgaagg agttaaaaat gagtcgtaaa gaatcccgag
     6781 tgcaagcttt tcaaacttta tttcaattag aaatgaagga cagtgattta acgataaatg
     6841 aagcgataag ctttattaaa gacgataatc cagatttaga cttcgaattt attcattggc
     6901 tagtttctgg cgttaaagat cacgaacctg tattagacga gacaattagt ccttatttaa
     6961 aagattggac tattgcacgt ttattaaaaa cggatcgtat tattttaaga atggcaacat
     7021 atgaaatatt acacagtgat acacctgcta aagttgtaat gaatgaagca gttgaattaa
     7081 caaaacaatt cagtgatgat gatcattata aatttataaa tggtgtattg agtaatataa
     7141 aaaaataaaa ttgagtgatg ttatatgtca gattatttaa gtgtttcagc tttaacgaaa
     7201 tatattaaat ataaatttga tcaagatcca catctccaat ctgtattgat taaaggtgag
     7261 ctttcaaatt tcaaaaagca ttcaagtggt catttatact ttaatgtgaa agataaagaa
     7321 agcgttataa gtgccatgat gttcaaaggt agtgcttcta aattaaactt cgaacctaaa
     7381 gaaggagatg aagtcttatt agaagcacgt gtttctgttt ttgaacgtcg tggaaactat
     7441 caaatttatg taaataaaat gcaattagat ggtataggga acttatatca aaaattagaa
     7501 gcattaaaga aaaaattaac tgaagaaggt tgttttgata aagcgaataa gaaatcaata
     7561 cctaagtttc caaaaaaaat tgcagtttta acagcgagta caggtgccgc aattcgagat
     7621 atccattcaa cgataaatag tcgtttccca ttagctgaac aaatacaaat tagtactttg
     7681 gttcaaggtg aaaaagcaaa ggacgacatt attgaaaaaa ttgaatatgc agatagttta
     7741 ggtgtagata ccattattgt aggtcgaggc ggtggatcca tagaagattt atggaatttt
     7801 aacgaagaag ctgtcgtccg tgcaatatat aattgtaaga caccgattat atcagcagta
     7861 ggtcatgaaa cagactttac attaagtgat tttgctgcag acatcagagc tgcgactcca
     7921 actcaagctg ctgttattgc aacaccagac caatatgaat tgctgcaaca aatacagcaa
     7981 tatcagttta cattgactcg tttcatcaag aaacatttag aacaacaacg taaacatgtt
     8041 gaacatttgt catcgtatta caagtttaaa caaccaactt tattgtatga tcaacagata
     8101 caacgtcgag atgatttaga aaagagactg aaacaacaaa ttcaggcaac ttttgaacaa
     8161 cagagacatc gattaatgct gttgcaacaa cgctataatt taaaagcgtt actaagtagt
     8221 gttaatcaag aacaacaaaa taatcttcaa ttgacaaacc aacttgttaa attattgaat
     8281 tctaagatat taagttataa aaatgatttg aaaaataaag tagaaaactt aaacaattta
     8341 agtccaacta atacaatgtt gcgtggatat gcaattgtta ataaaaaaga cgaagtcatt
     8401 acgagtacta aagatttaac agaaaatgat caattgacgt taacaatgaa agatggctta
     8461 gtagatgcaa aagttacgaa agtaaggtgt aataatgact aaagaaacgc aaagttttga
     8521 agaaatgatg caagaattag agcaaattgt tcaaaaatta gataatgaaa cagtatcttt
     8581 agaggaatca ttagatttat accaacgtgg tatgaaacta tcagcagctt gtgacacaac
     8641 tttaaaaaat gccgaaaaaa aggtgaatga cttaataaaa gaagaagctg aggatgtaaa
     8701 aaatgacgaa tctaccgatg aataaattaa tagatgaagt caataatgaa ttatcggttg
     8761 cgataaataa atcagtaatg gatactcagc tagaagaaag tatgttgtat tcattaaatg
     8821 ctggaggtaa acgcatccga ccagttctgt tattactcac tttagattca ctaaataccg
     8881 agtatgagtt aggtatgaag agcgcaattg cactagaaat gattcataca tattcactta
     8941 ttcatgatga cctaccagcg atggataatg atgattatcg acgagggaaa ttaacaaatc
     9001 ataaagtata tggtgagtgg actgcgatat tagcaggtga tgctttatta actaaagcat
     9061 ttgaacttat ttcaagtgat gatagattaa ctgatgaagt aaaaataaaa gttctacaac
     9121 ggctgtcaat agcaagtggt catgttggaa tggtcggtgg acaaatgtta gatatgcaaa
     9181 gcgaaggcca accaattgat cttgaaactt tggaaatgat acacaaaaca aaaacaggtg
     9241 cactattaac ttttgcggtt atgagtgcag cagatatcgc taatgtcgat gatgcaacta
     9301 aagaacattt agaaagttat agttatcatt taggtatgat gtttcagatt aaagatgatt
     9361 tattagactg ctatggtgat gaagcgaagt taggtaaaaa agtgggcagc gatcttgaaa
     9421 ataataaaag tacgtacgtg agtttattag gaaaagatgg cgcagaagat aaattgactt
     9481 atcatagaga cgcagcagtg tatgaactaa cgcaaattga tgaacaattc aatacaaaac
     9541 acttattaga aatcgttgat ttattttata gcagggatca ctaaaagcaa atatatcgat
     9601 tagcaacaat tgtaaagttt gaaatgtctc aaaaggcata ttcaactgac gttgtttgtt
     9661 aatcgatttt tctcatgttg aaacttaaaa ttatgttgaa gtctttcatt taaaaataga
     9721 attttcaatt tctatacgac aatattcgca tctgtgatat caatgtgatt ttaattaaat
     9781 atgttcaaac aattgacgct tattaaa
//
LOCUS       DAHHOJ010000098         9575 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.098, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000098 DAHHOJ010000000
VERSION     DAHHOJ010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9575
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.098"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(292..2748)
                     /locus_tag="NBH32_002103"
     CDS             complement(292..2748)
                     /locus_tag="NBH32_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HCD1515682.1"
                     /translation="MLFGRLTERAQRVLAHAQEEAIRLNHSNIGTEHLLLGLMKEPEG
                     IAAKVLESFNITEDKVIEEVEKLIGHGQDHVGTLHYTPRAKKVIELSMDEARKLHHNF
                     VGTEHILLGLIRENEGVAARVFANLDLNITKARAQVVKALGNPEMSNKNAQASKSNNT
                     PTLDSLARDLTVIAKDGTLDPVIGRDKEITRVIEVLSRRTKNNPVLIGEPGVGKTAIA
                     EGLAQAIVNNEVPETLKDKRVMSLDMGTVVAGTKYRGEFEERLKKVMEEIQQAGNVIL
                     FIDELHTLVGAGGAEGAIDASNILKPALARGELQCIGATTLDEYRKNIEKDAALERRF
                     QPVQVDEPSVVDTVAILKGLRDRYEAHHRINISDEAIEAAVKLSNRYVSDRFLPDKAI
                     DLIDEASSKVRLKSHTTPNNLKEIEQEIEKVKNEKDAAVHAQEFENAANLRDKQTKLE
                     KQYEEAKNEWKNAQNGMSTSLSEEDIAEVIAGWTGIPLTKINETESEKLLSLEDTLHE
                     RVIGQKDAVNSISKAVRRARAGLKDPKRPIGSFIFLGPTGVGKTELARALAESMFGDD
                     DAMIRVDMSEFMEKHAVSRLVGAPPGYVGHDDGGQLTEKVRRKPYSVILFDEIEKAHP
                     DVFNILLQVLDDGHLTDTKGRTVDFRNTIIIMTSNVGAQELQDQRFAGFGGSSDGQDY
                     ETIRKTMLKELKNSFRPEFLNRVDDIIVFHKLTKEELKEIVTMMVNKLTNRLSEQNIN
                     IIVTDKAKDKIAEEGYDPEYGARPLIRAIQKTIEDNLSELILDGNQIEGKKVTVDHDG
                     KEFKYDIAEQTSETKTPSQA"
     gene            complement(2762..3769)
                     /locus_tag="NBH32_002104"
     CDS             complement(2762..3769)
                     /locus_tag="NBH32_002104"
                     /EC_number="2.7.14.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein arginine kinase"
                     /protein_id="HCD1515683.1"
                     /translation="MTHNIHDNISQWMKSNEETPIVMSSRIRLARNLENHVHPLMYAT
                     ENDGFRVINEVQDALPNFELMRLDQMDQQSKMKMVAKHLISPELIKQPAAAVLVNDDE
                     SLSVMINEEDHIRIQAMGTDTTLQALYNQASSIDDELDKSLDISYDEQLGYLTTCPTN
                     IGTGMRASVMLHLPGLSIMKRMTRIAQTINRFGYTIRGIYGEGSQVYGHTYQVSNQLT
                     LGKSELEIIETLTEVVNQIIHEEKQIRQKLDTYNQLETQDRVFRSLGILQNCRMITME
                     EASYRLSEVKLGIDLNYIELQNFKFNELMVAIQSPFLLDEEDDKSVKEKRADILREHI
                     K"
     gene            complement(3759..4325)
                     /locus_tag="NBH32_002105"
     CDS             complement(3759..4325)
                     /locus_tag="NBH32_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000882074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HCD1515684.1"
                     /translation="MLCENCQLNEAELKVKVTSKNKTEEKMVCQTCAEGHHPWNQANE
                     QPEYQEHQDNFEEAFVVKQILQHLATKHGINFQEVAFKEEKRCPSCHMTLKDIAHVGK
                     FGCANCYATFKDDIIDIVRRVQGGQFEHVGKTPHSSHKKIALKRKIEEKNEYLKKLIE
                     IQDFEEAAIVRDEIKALKAESEVQHDDA"
     gene            complement(4344..4805)
                     /locus_tag="NBH32_002106"
     CDS             complement(4344..4805)
                     /locus_tag="NBH32_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HCD1515685.1"
                     /translation="MHNMSDIIEQYIKRLFEESNEDVVEIQRANIAQRFDCVPSQLNY
                     VIKTRFTNEHGYEIESKRGGGGYIRITKIENKDATGYINHLLQLIGPSISQQQAYYII
                     DGLLDKMLINEREAKMIQAVIDRETLSMDMVSRDIIRANILKRLLPVINYY"
     gene            4963..6177
                     /locus_tag="NBH32_002107"
     CDS             4963..6177
                     /locus_tag="NBH32_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCD1515686.1"
                     /translation="MHILIGIIGIIFFLALAFLFSSDRKNIRWKYVGILLAIQLIFAF
                     ILLKTTMGIKVIGGISNGFNYLLLKAADGVNFVFGGIQYIDPKQPPFFFSVLLPIVFI
                     SAIIGILQYTKILPLIINVLGFLISKINGMGRLESYNAVAAAILGQSEVFISIKKQLP
                     YIPKQRLYTLTASAMSTVSASIIGAYFTLIEPKYVVTAVVLNLFGGFIIASIINPYKV
                     NEEDDKLLVEETEERQQSFFEMLGEYILDGFKVAVIVGAMLIGYIAIIALLNGIVSNI
                     FSTVSGGAISWDFQTLIGFVFAPFAFLVGVPWQDAVQAGSVMATKLLSNEFVAMQALG
                     KLSDLSEHAKGVTSVFLVSFANFSSIGIISGAIKSLNDKKGDTVARFGLKLLFGATLV
                     SFISAAIAGFFI"
     gene            complement(6385..6945)
                     /gene="pdxT"
                     /locus_tag="NBH32_002108"
     CDS             complement(6385..6945)
                     /gene="pdxT"
                     /locus_tag="NBH32_002108"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HCD1515687.1"
                     /translation="MKIGVLALQGAVREHIRHIELSGHEGIAVKKVEQLEEIEGLILP
                     GGESTTLRRLMNLYGFKEALQNSTLPMFGTCAGLIVLAQDIVGEEGYLNKLNITVQRN
                     SFGRQVDSFETELDIKGIATDIEGVFIRAPHIEKVGQGVNILCKVNEKIVAVQQGKYL
                     GVSFHPELTDDYRVTDYFINHIVKKA"
     gene            complement(6949..7836)
                     /gene="pdxS"
                     /locus_tag="NBH32_002109"
     CDS             complement(6949..7836)
                     /gene="pdxS"
                     /locus_tag="NBH32_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011303857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HCD1515688.1"
                     /translation="MSKIIGSDRVKRGMAEMQKGGVIMDVVNAEQARIAEEAGAVAVM
                     ALERVPSDIRAAGGVARMANPKIVEEVMNAVSIPVMAKARIGHITEARVLEAMGVDYI
                     DESEVLTPADEEYHLRKDQFTVPFVCGCRNLGEAARRIGEGAAMLRTKGEPGTGNIVE
                     AVRHMRQVNSEVSRLTVMNDDEIMTFAKDIGAPYEILKQIKDNGRLPVVNFAAGGVAT
                     PQDAALMMELGADGVFVGSGIFKSEDPEKFAKAIVQATTHYQDYELIGRLASELGTAM
                     KGLDINQLSLEERMQERGW"
     gene            7940..9322
                     /locus_tag="NBH32_002110"
     CDS             7940..9322
                     /locus_tag="NBH32_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HCD1515689.1"
                     /translation="MKNTLYHQLYEKLKKQIIEGQFKEGDKFYSKRQLSKHLSISQTT
                     VEHAYQLLLDEGYIYSRPRSGYFVSEIESLTILNNQPIPSLFDDDSYKPKASDEAYDY
                     AFNLDEIDTKHFPIELFRKYSKDLYDTNHLNQLRRGHFQGELHLRFQLAFYLFTNRGV
                     ICDPNQIIIGSSTEQLVNQLVDLLYTSTFIIEKPSYPPIKNILDKKQVEYEQIEVEDN
                     GINVDEVIKSQKNIVYITPSHQFPTGYVMDLKKRTQLIQWAQEKEERFIIEDDYDSEF
                     RYFGKPIPAIQGLYSRGEKVIYISTFSKSIFPSCRVAYMVLPYSIMKKYHSQNHIEGN
                     TVPVHMQNLIATFISSGGFERHLNKMRRIYRRKLTYILKRLKPYKEQLDIQGAETGMH
                     FTITVKNGLTLQECLDRANKVKLKLQVYNFDDDQDYKKTPKFILGFGGIDDDALKPHV
                     DALIKTLIIK"
BASE COUNT         2955 a         1768 c         1336 g         3516 t
ORIGIN      
        1 atcttaataa tttcttagta aactcttttc tctagattta tcacaatatt atatagacct
       61 attttatttt gacgtaagtt gctagtatct tcaaacaaaa aacctttata aaaattcata
      121 cctttatgct atcgctgtag gctcattaac ttgtttcata taattcttaa ctatcctttg
      181 atgattattt tattagattg tttcgttgat ggatactttc acgaatttct atagttcaat
      241 gctactaaaa aaacagccca aaactttaat ttgttttgga ctgttttata attatgcttg
      301 cgatggtgtt ttagtttctg aagtttgttc agcaatgtca tatttaaact ctttaccatc
      361 atgatctact gtaactttct taccttcaat ttgattacca tctaatatta attcacttaa
      421 attatcttcg atagtttttt gtatcgctct aattaatggt cttgcaccat attctggatc
      481 atatccttct tctgcgattt tgtctttcgc tttatcagta acaataatat ttatgttttg
      541 ttcagataat cgatttgtta atttattaac catcattgtt acaatttctt ttaattcttc
      601 ttttgttagt ttatggaata caatgatatc atctacacgg tttaaaaatt ctggacggaa
      661 tgaatttttt aattctttta acatcgtttt tcgaattgtt tcataatctt gtccatcact
      721 tgaaccaccg aatccagcaa atcgttgatc ttgtaattct tgtgctccaa cgtttgatgt
      781 cattatgata attgtatttc tgaaatcaac tgtacgtcct tttgtatctg tcaaatgtcc
      841 gtcatctaaa acttgtaata gaatattaaa tacatctgga tgtgcttttt caatttcatc
      901 aaataaaatt acagaatacg gtttacgtct aactttttca gttaattgtc caccatcatc
      961 atgaccaaca tatcctggag gagcaccaac taatcggctc actgcgtgtt tttccataaa
     1021 ttcactcatg tctacacgga tcatcgcatc atcatcgcca aacattgatt cagctaaagc
     1081 tctagctaat tcagttttac caacaccagt tggtccaagg aagataaagc taccaattgg
     1141 tcgtttagga tcttttaacc ctgcacgggc acgtctaacc gctttactga ttgaattaac
     1201 agcatctttt tgcccaataa ctctctcatg taatgtatct tctagactaa gaagtttttc
     1261 agattctgtt tcattgattt tagttaatgg gatacctgtc catcctgcaa taacttcagc
     1321 aatatcttct tctgacaatg aagttgacat gccattttgt gcattcttcc attcattttt
     1381 agcttcttca tattgctttt caagttttgt ttgtttatca cgcaggttag cagcattttc
     1441 aaactcttga gcatgtactg cggcatcttt ttcattttta actttttcaa tttcttgttc
     1501 aatttctttt aaattattag gtgtcgtatg actcttaagt cttactttag aacttgcttc
     1561 atcaattaaa tcaattgctt tatctggtaa gaaacgatct gaaacgtatc tgttacttaa
     1621 tttaacagct gcttcaatag cttcgtctga aatattaata cgatggtgtg cttcgtaacg
     1681 atctcttaat ccttttaaaa tagcaactgt atctactact gaaggttcat caacttgtac
     1741 aggttggaaa cgacgttcta aagccgcgtc tttttcaata tttttgcgat attcatctaa
     1801 tgtagtagca ccaatacatt gtaattcacc acgtgctaat gccggcttca aaatattcga
     1861 agcatcgata gcaccttcag caccaccagc accaactaaa gtatgcaact catcaataaa
     1921 taggatgaca ttacctgctt gttggatttc ttccataacc tttttcagac gctcttcaaa
     1981 ttcaccacga tatttagtac ctgcaactac tgttcccata tctaaagaca taacacgttt
     2041 atcttttaat gtctctggta cctcattatt cactatggct tgcgctaaac cttcagcaat
     2101 agcagtttta ccaacacctg gctctccaat aagcacagga ttgtttttcg tacgtctact
     2161 taatacttca attacacgtg taatttcttt atcacgtcct ataacaggat ctaatgtacc
     2221 gtctttggca atgactgtta agtcacgagc taaactatct aaagttggag tattatttga
     2281 cttactagct tgtgcatttt tattactcat ttcagggttt cctaaagctt tcacaacttg
     2341 tgcacgtgct ttagtaatat ttaaatctag atttgcaaaa actcttgctg caacaccttc
     2401 attttcacga atcaagccta ataaaatatg ttccgttcca acaaaattgt gatgtaattt
     2461 tctagcttca tccatcgata attcaatgac ttttttagct ctaggtgtat aatgcaatgt
     2521 accaacatga tcttgaccat gtccgattaa tttttcaact tcttcaatta ctttatcttc
     2581 agtgatatta aaactttcta atacttttgc agcaattcct tcaggttctt tcattaatcc
     2641 caataatagg tgttctgttc ctatatttga atgatttaaa cgaattgctt cttcttgtgc
     2701 atgtgctaat acgcgctgtg cacgctcagt taatctacca aataacataa ataatgacct
     2761 cctactttat atgttctctt agtatatctg ctcgtttttc ttttacagat ttgtcatctt
     2821 cttcatctaa taaaaatggt gactgtatag ctaccatcaa ttcattaaat ttaaagtttt
     2881 gtaattcaat gtaatttaaa tctataccaa gtttaacttc gcttaatcta taagaagcct
     2941 cttccatagt tatcattcta cagttttgta aaatacctag cgagcgaaaa acacggtctt
     3001 gtgtttctaa ttgattataa gtgtctaact tttgtcgtat ttgtttttct tcatgaatga
     3061 tttgattaac aacttctgtt aatgtttcta tgatttctaa ctcagattta ccaagtgtaa
     3121 gttggttgga tacttgataa gtatgtccat aaacttgcga accttcaccg taaatacctc
     3181 tgattgtata tccaaaacga ttaatggttt gagcaatccg tgtcattctt ttcataatag
     3241 atagacctgg taaatgtagc atcacgcttg ctctcatacc agtacctata ttggtaggac
     3301 atgtagttaa ataaccaagt tgttcatcat aacttatatc aaggctttta tctaattcat
     3361 catcaattga tgaagcttga ttatataaag cctgtaatgt cgtgtcagtt cccatagctt
     3421 gaatacgaat atggtcctct tcatttatca tgacacttaa agattcatca tcattcacta
     3481 atactgcggc tgctggttgt tttattagtt caggactaat caaatgcttt gcaaccatct
     3541 tcattttact ttgttgatcc atttgatcaa gacgcattaa ttcaaaattt ggcaaggcat
     3601 cttgtacctc atttataact ctaaatccat cattttcagt agcatacatt agtggatgca
     3661 catgattttc taaattacgc gctaaccgaa ttctagaaga cataacaatg ggtgtttctt
     3721 cattactttt catccattgg ctgatattat catgaatatt atgcgtcatc atgttgcacc
     3781 tcactctcag cttttagtgc tttaatttca tctctaacaa tggctgcttc ctcaaaatct
     3841 tggatttcaa taagtttttt caaatattca ttcttttctt cgatttttcg ctttaaagct
     3901 atctttttat gtgaagaatg tggtgtcttt ccaacgtgct caaattgtcc accttgaact
     3961 ctgcggacga tatcaatgat gtcatcttta aatgttgcat aacaattagc acacccaaat
     4021 ttaccaacat gtgcaatatc tttcaaagtc atatgacatg atgggcaacg tttttcttct
     4081 ttaaacgcta cttcttgaaa attaatgcca tgtttcgtag ctaaatgttg taaaatttgc
     4141 ttaacaacaa atgcttcttc gaaattatct tgatgttctt gatattcagg ttgttcatta
     4201 gcttgattcc acggatggtg cccctcagca caagtttgac acaccatttt ttcttctgtt
     4261 ttatttttac ttgtaacttt aacttttaat tccgcttcat taagttgaca attttcacaa
     4321 agcacttcaa cacctcattt catttagtaa taatttataa ctggtaacaa acgttttaaa
     4381 atatttgctc taataatatc tctagaaacc atatccattg atagcgtttc tctatcaata
     4441 actgcttgaa tcattttagc ttcacgttca tttattaaca ttttatctaa aagcccatca
     4501 ataatataat aagcttgttg ttgagaaata gaaggtccaa tcagctgaag caaatgatta
     4561 atataacctg ttgcatcttt attttcaatt ttagtgattc ggatgtaacc accaccacca
     4621 cgtttacttt cgatttcata accatgttca ttagtgaatc gtgttttgat tacataattt
     4681 aattgtgatg gtacacaatc aaagcgctgt gcgatattcg ctctctgaat ttcaacgaca
     4741 tcttcattcg actcttcaaa taaacgtttg atgtattgtt ctatgatgtc agacatattg
     4801 tgcatgtata tcaccccttt ttgaccttct ttgactatat tatatgacct attttgacct
     4861 ttttcaatta atttgttttt atattttcaa aaatttatga tgttagccct tccattttat
     4921 tgtatgatag attgtagaaa aaactaaagg aatgagaaat atatgcatat tctaattggg
     4981 attatcggaa ttatcttctt tttagcactt gcgtttctat ttagctcaga caggaaaaat
     5041 attcgctgga aatatgtagg aattttactt gcaattcagc ttatctttgc atttatcttg
     5101 cttaaaacta caatgggaat caaggttatt ggtggtattt caaacggttt taactactta
     5161 ttattaaaag cagcagatgg tgttaacttt gtattcggtg gtattcaata tatcgatcct
     5221 aagcaaccac cattcttctt cagtgtatta ttacctatcg tatttatttc agcgattatc
     5281 ggtatccttc aatacacaaa gattttacct ttaatcatta atgttttagg attcttaatt
     5341 tctaaaatta atggtatggg acgtttagaa tcatacaatg cagttgctgc agcaattttg
     5401 ggacaatctg aagtatttat ttcaattaaa aaacaattac catacatacc taagcaacgt
     5461 ttatacacat taacagcttc agcaatgtcg actgtttctg cttcaattat cggtgcatac
     5521 tttaccttaa ttgaacctaa atatgttgta acggctgttg tcttaaactt atttggtggt
     5581 ttcatcatcg cttcaattat taacccttat aaagttaatg aagaagatga caaattatta
     5641 gttgaagaaa ctgaagaaag acaacaatca ttcttcgaaa tgctaggaga atatatttta
     5701 gatggattta aagtagctgt cattgtaggc gcaatgttaa tcggatacat tgctattatc
     5761 gctctattaa atggaattgt aagcaatatc tttagtacag tttcaggtgg cgctatttct
     5821 tgggatttcc aaacattaat tggttttgtc tttgcaccat ttgcattctt agtcggtgtg
     5881 ccatggcaag atgcagttca agccggttca gttatggcga caaaactatt atctaacgaa
     5941 tttgtagcaa tgcaagcact cggtaaactt agtgatttat ctgaacacgc aaaaggtgtt
     6001 acatctgtat tcttagtatc atttgctaat tttagctcta ttggtattat ttcaggtgcg
     6061 attaaatctc ttaatgacaa aaaaggagat acagttgccc gctttggttt gaaattatta
     6121 ttcggtgcaa cactagtatc tttcatttca gctgcaatcg ctggattctt tatttaacaa
     6181 atacatcaac atcgaattaa ccccttcaaa attttgacta ttctcagtgt ctttgaaggg
     6241 gttttttctt atgtatatct ttatttttta tcatttttaa ttttgacata aaaaacaact
     6301 cctccaaagc taagtttgaa ggagatattg tcttctcaac aaatctataa atatcaataa
     6361 ttcgttgatt tagcatacat taagctatgc tttttttaca atatgattaa taaagtaatc
     6421 agttactcta tagtcatctg ttaattcagg atggaatgat acgcctaaat atttaccttg
     6481 ctggacagct acaattttct cattaacctt acataggata tttacgcctt gacctacttt
     6541 ttcaatgtgt ggggctctta taaagacacc ttcaatatct gtagcgatac ctttaatatc
     6601 taattctgtt tcaaagctgt caacttgtct accgaatgag tttcgttgta cagtaatatt
     6661 caacttgtta aggtatcctt cttcaccaac tatatcttgc gctagaacta ttaatcctgc
     6721 gcatgtacca aacataggta aagttgaatt ttgtaaagcc tctttaaatc catataaatt
     6781 cattaatcga cgtaacgttg tagactcgcc accaggtaat attaagccct cgatttcttc
     6841 taattgttca acttttttaa ctgcaatacc ttcatgacca cttaattcaa tatgtctaat
     6901 atgttcacgt actgcacctt gtaatgctaa tacacctatt ttcatatctt accaaccacg
     6961 ctcttgcata cgttcttcta atgataattg attgatatct aaacctttca tagcagtgcc
     7021 aagttcactt gctaatcttc caattagttc atagtcttgg taatgtgttg ttgcttgaac
     7081 aattgcttta gcaaattttt ctggatcttc tgatttaaaa atacctgatc caacgaatac
     7141 accatcggca cctaattcca tcattaaagc agcatcttga ggagtcgcaa cgccaccagc
     7201 tgcaaagtta actaccggta aacgaccatt gtctttaatt tgttttaaaa tttcataagg
     7261 cgcaccgata tctttcgcga aagtcataat ctcatcatca ttcattacag tcaatcgact
     7321 aacttctgaa ttaacttgtc tcatatgtct tacagcttca acaatattac ctgtacctgg
     7381 ttcaccttta gtacgtaaca tagcagcacc ttcaccaatt ctacgcgcag cttcacctaa
     7441 attacgacat ccacatacaa atggtactgt aaattgatct tttcttaagt gatattcctc
     7501 atctgctggt gttaacactt ctgattcatc aatatagtca acacccatcg cctctaatac
     7561 tcttgcttca gtgatatgac caatacgtgc tttagccatg actggaatag aaacagcatt
     7621 cattacttct tctacaattt tagggtttgc catacgtgca acgccaccag cagctctaat
     7681 atcagaaggt actcgttcta atgccataac tgctaccgcg ccagcttctt ctgcaattct
     7741 tgcttgctca gcattaacga catccataat aacgccgcct ttttgcattt cagccatacc
     7801 tcttttgact ctgtctgatc caataatttt actcattata cataaaaccc cttttcaagt
     7861 tgattaaata cattgtaatg tataaactga tatttaaaaa ggtacaaaat aagacaaatt
     7921 taggaggtca gttataaaat tgaaaaacac tttataccat caactttatg aaaaattaaa
     7981 aaaacaaata attgaaggtc aatttaaaga aggtgataaa ttttattcta aaagacaatt
     8041 aagcaaacat ttatctatca gccaaacaac cgtagaacat gcatatcaac ttctattaga
     8101 tgaaggttac atttattcaa gacctcgctc tggatatttt gtgtccgaaa ttgaatcttt
     8161 aacaatttta aacaatcaac ctatcccctc tctttttgat gatgattcat ataaaccaaa
     8221 agcgagtgat gaagcttatg attatgcttt taatttggat gaaatagata cgaaacattt
     8281 ccctatagaa cttttcagaa agtattccaa agatctttat gatacaaatc atttgaacca
     8341 attacgtaga ggacattttc aaggagaatt acatttaaga tttcaactgg cattttattt
     8401 atttactaat cgtggtgtaa tttgtgatcc taatcaaatt ataattggct catctaccga
     8461 acaattagta aatcaattag tcgatttact ttatacttca acgtttatta tcgaaaaacc
     8521 tagttatcca ccaatcaaaa atattttaga taaaaaacaa gttgaatatg aacaaattga
     8581 agttgaagat aatggaataa acgtagatga agttattaaa tcacaaaaaa atatagtata
     8641 tataacgcct tcccatcaat ttccaactgg atatgtgatg gacttaaaaa agcgaactca
     8701 attaatacaa tgggctcaag aaaaagaaga acgatttatt atagaagatg attatgattc
     8761 tgagtttaga tatttcggta aacccattcc agcaatacaa ggtctatatt caagaggaga
     8821 aaaagtgatt tatatcagta cattttcaaa gtctattttt cctagctgtc gtgttgcata
     8881 tatggtgtta ccctattcta taatgaaaaa atatcattct caaaatcaca ttgaaggcaa
     8941 cactgtacct gttcatatgc aaaaccttat tgcaacgttt atatcttccg gtggttttga
     9001 aaggcattta aataaaatga gacgaattta tcgtagaaaa ctaacctata ttttaaaaag
     9061 attgaagcca tacaaagagc aacttgatat tcaaggtgct gaaacaggta tgcattttac
     9121 aattacagta aaaaatggat taacattaca agagtgtctt gatagagcaa ataaagtaaa
     9181 gttaaaatta caagtttata attttgatga tgaccaagac tataagaaaa cccctaaatt
     9241 tatattaggc tttggcggta tagacgatga tgctttaaaa ccgcatgtag acgctctaat
     9301 aaaaacatta attataaagt aacacttctt tgataaaagt tagtgttact tatgcaatag
     9361 cttgtctatt gtataataat taatttcctt tttgtatttc aataaagtat ataaaagagc
     9421 agtaagatca ttttcaatta gataccgtct tactgctctt ctctatttat acaatatttt
     9481 gttttcgatg atatcatttt aaatttatat aaacacaaaa ttaaagttgt acatacttag
     9541 acaatcaaaa gtgcacatta ttaaattatc atttc
//
LOCUS       DAHHOJ010000099         9498 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.099, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000099 DAHHOJ010000000
VERSION     DAHHOJ010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9498)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9498)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9498
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.099"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            185..649
                     /locus_tag="NBH32_002111"
     CDS             185..649
                     /locus_tag="NBH32_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1515690.1"
                     /translation="MDRQSFTDLIQTKFKMVRIEAGYTQDTMAQTIGLSKKTLVQIEK
                     ERVLPNWTTCISICALFRDSEVLNSTFGCDPLEIVQTISRNHCAYPNHAPTSDIYWNN
                     IETRNGYILQSNKVSNIYRVLNPDNQPIFGTSKMREAETYFNRNAKEELVHI"
     gene            828..1952
                     /locus_tag="NBH32_002112"
     CDS             828..1952
                     /locus_tag="NBH32_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF445 domain-containing protein"
                     /protein_id="HCD1515691.1"
                     /translation="MNALFIIIFMIVVGAIIGGITNVIAIRMLFHPFKPYYIFKFRVP
                     FTPGLIPKRREEIATKIGQVIEEHLLTETLINEKLKSEQSQQAIESMIQQQLQKLTKD
                     QLSIKQITSQLDIDLEQVLQTNGNQYIESQLNNYYTKHQNQTIASLLPNQLVTFLDQH
                     VDNATDLLCDRARNYLSSAKGTQDINDMLDTFFNEKGKLIGMLQMFMTKESIADRIQQ
                     ELIRLTSHPKARTIVTSLITNEYQTFKDKPLNELLDAAQFNEIAENLSVYVTTYASKQ
                     ANKPVVTLMPQFVDYLEGQLSSKLANLIIEQLSIHLSTIMKKVDLRGLIEEQINTFDL
                     DYIEKLIIEIANKELKLIMSLGFILGGIIGFFQGLVAIFV"
     gene            2021..2365
                     /locus_tag="NBH32_002113"
     CDS             2021..2365
                     /locus_tag="NBH32_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002498160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF/YmcA family competence regulator"
                     /protein_id="HCD1515692.1"
                     /translation="MAVNLYDYANQLEQALRESEEYKAIKEAFANVKANEESKKLFDE
                     FRETQINFQQKQMQGEEIAEEDLQKAQEQAQAIEKDENISALMNAEQKMSQVFQEINQ
                     IIVKPLDEIYAD"
     gene            3137..4333
                     /locus_tag="NBH32_002114"
     CDS             3137..4333
                     /locus_tag="NBH32_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD1515693.1"
                     /translation="MVKFIHCSDLHLDSPFKSKSHISPKIFEDVQKSAYESFKNIVDI
                     ALQQDVDFVIIAGDLFDSENRTLRAEIFLKQQFERLQNEQIFVYVCHGNHDPLSSKIS
                     SNWPDNVSVFSNKVETYEAITKSGETIYIHGFSYENRASYENKIDEYPSSQGQKGIHI
                     GVLHGTYSKSSVNERYTEFILEDLNSKLYHYWALGHIHERQQLSDMPVINYSGNIQGR
                     HFNEQGEKGCLLIEGDHLKLKTKFYPTQYIRFEEATIETDKTSKQGLYEVIQNFKEQV
                     REEGKAFYRLTLVINSETLISPQDLLQVEEMITDYEENENQFVYIDELKIQYAQNDES
                     PLVNEFSAELLVDQTVFDKAMSDLYLNPRASKFLDDYGTFDHTALVNRAEEILKAEMR
                     GEQNDN"
     gene            4323..7259
                     /locus_tag="NBH32_002115"
     CDS             4323..7259
                     /locus_tag="NBH32_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCD1515694.1"
                     /translation="MIIKSLEIYGYGQFVQRKIEFNKNFTEIFGENEAGKSTIQAFIH
                     SILFGFPTKKSKEPRLEPRLGNQYGGKLVLILDDGLEIEVERIKGSAQGDVKVYLPNG
                     AVRDDAWLQKKLNYISKKTYQGIFSFDVLGLQDIHRNLNEKQLQDYLLQAGALGSTEF
                     TSMREVINRKKDELYKKSGKNPIINQQIEQLKQLESQIREEEAKLETYHRLVDDRDKS
                     SRRLENLKHNLNQLSKMHEEKQKEVALHDHSQEWKSLEQQLNIEPITFPEKGVDRYEK
                     ARAHKQSLERDIGLRNERLAQLKEEATQLEPVKQSDIDAFISLNQQENEIKNKEFELT
                     AIEKDIANKQRDKDELQANIGWSETHHDVDSSEAMKSYVSEQIKNKQEQAAYIKQLER
                     SLEGNKIEDNAVHSELDSVEKKIVPEETFEKKKEYSQQVIELNEKENLYSKLKERFEI
                     EQQEKQKRQKLLRTTFILLTLVGIGLTAFSFISNNMLFGIIFAVLTLVFVIGIIMSKS
                     KEVDYSEAITDEIEEIKAQLAILDENYDLDFDLDEQYRIRDHWQQALKNKDILEEKRQ
                     YIEGRLNDAKGRHDELQSTVENVKDELYLSSKISNDLIVDSISTMANIKALDQHISDL
                     NQQRQQLVQELDTFYNHAEAVTKSQFVYFNKLSLFHDVQQWLKSAEDTNEKWRINAEN
                     TKLVTNELNHLNAQLEENNKEITALFDFINVGTEEDFYQHHEDYQTYTSNLSRFNDLT
                     KYLENQNYSYELSSSLSEKTTAQLEEEDHLLATQVDEYNEQYLEMQAQVSDLSAQINH
                     METDTTLANLRHEYHSLKNQLNDIAKDWASLSYLQSLVDEHIKQIKDKRLPQVINEAV
                     EILKHLTDGRYTMINYNEDSITVKHVNGQLYDPVELSQSTKELLYVALRISLIKVLRP
                     YYPFPLIVDDAFVHFDKKRTEKMLNYLRSLSEHYQVLYFTCVKDNIVPSKEVITLNKI
                     EEGGKR"
     gene            7256..8197
                     /gene="yhaM"
                     /locus_tag="NBH32_002116"
     CDS             7256..8197
                     /gene="yhaM"
                     /locus_tag="NBH32_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease YhaM"
                     /protein_id="HCD1515695.1"
                     /translation="MRNIENLNPGDSVDHFFLVHKATQGVTAQGKDYMTLHLQDKSGE
                     IEAKFWTATKNDMATIKPEEIVHVKGDIINYRGNKQMKVNQIRLATTEDQLKTEQFVD
                     GAPLSPAEIQEEISHYLLDIENANLQRITRHLLKKYQERFYTYPAASSHHHNFASGLS
                     YHVLTMLRIAKSICDIYPLLNKSLLYSGIILHDIGKVRELSGPVATSYTVEGNLLGHI
                     SIASDEVVEAARELNIEGEEIMLLRHMILSHHGKLEYGSPKLPYLKEAEILCYIDNID
                     ARMNMFEKAYKKTDKGQFTDKIFGLENRRFYNPESLD"
     gene            complement(8318..9280)
                     /locus_tag="NBH32_002117"
     CDS             complement(8318..9280)
                     /locus_tag="NBH32_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="foldase"
                     /protein_id="HCD1515696.1"
                     /translation="MKMINKLIVPVTASALLLGACGASATDSKENTLISSKAGDVTVA
                     DTMKKIGKDQIANASFTEMLNKILADKYKNKVNDKKIDEQIEKMQKQYGGKDKFEKAL
                     QQQGLTADKYKENLRTAAYHKELLSDKIKISDSEIKEDSKKASHILIKVKSKKSDKEG
                     LDDKEAKQKAEEIQKEVSKDPSKFGEIAKKESMDTGSAKKDGELGYVLKGQTDKDFEK
                     ALFKLKDGEVSDVVKSSFGYHIIKADKPTDFNSEKQSLKEKLVDQKVQKNPKLLTDAY
                     KDLLKEYDVDFKDRDIKSVVEDKILNPEKLKQGGAQGGQSGMSQ"
BASE COUNT         3545 a         1344 c         1593 g         3016 t
ORIGIN      
        1 ttcgcttcat tataaagtaa acttaactct tgttgacttg atcaaaattt aatacaaatg
       61 tgaaattgac tcttttcatt ttattaaaaa tatattaaga tgtaatctgt gtttcaagaa
      121 atgtgtatta ttgcaatttc ttgtgtccgg ataaaattcc ccaaacagaa aggtagataa
      181 cagaatggat agacagagtt ttacagattt aattcaaaca aaatttaaaa tggttcgtat
      241 agaggctggt tatacgcaag atactatggc gcaaacaatt ggactttcta aaaagacttt
      301 agtacaaatc gaaaaagaga gagtattacc aaactggaca acttgtattt caatttgtgc
      361 attattcaga gactctgaag tattaaacag tacatttggc tgtgatccat tagaaatcgt
      421 tcaaacaatt tcaagaaatc actgtgcata tccaaaccat gcaccaacaa gtgatattta
      481 ttggaataac attgagactc gtaatgggta cattttacaa agtaacaaag taagcaatat
      541 ttatcgtgta ttaaatccag ataaccaacc aattttcggt acttctaaaa tgagagaagc
      601 tgaaacatac tttaacagaa atgctaaaga agaattagta catatctagg cctatatact
      661 tttaataaat aataaaagcg atgtgacaca cctgtttcga gaggtgaggc atcgcttttt
      721 gtatgtaaac taataataaa ttgagcagaa agtgaaatga ttaatgaatt aaaaaataaa
      781 aatagtggta taatgaataa ctatgttgtc acaaaggagt caaaattatg aatgcactat
      841 ttattatcat atttatgatt gtagtagggg cgataattgg tggcattact aatgtaattg
      901 caattagaat gctgtttcac ccctttaaac catactatat atttaaattt agagtcccat
      961 ttacaccagg tttaataccg aaaagacgcg aagaaattgc aactaaaatt ggtcaagtga
     1021 ttgaagagca tttgcttaca gaaactttaa ttaatgaaaa attaaaaagc gagcaatcac
     1081 agcaagcaat agaatctatg attcaacaac agttacaaaa gttgacgaaa gatcaattgt
     1141 caataaaaca aattacttct caactcgata ttgatttaga acaagtatta caaactaatg
     1201 gaaatcaata tattgaatca caattgaata attattatac aaagcatcaa aaccaaacaa
     1261 tagcatcttt attgccaaat caacttgtaa catttttaga tcagcatgta gataacgcaa
     1321 cagacttatt atgtgatcgt gcaagaaatt atttatcatc tgcaaaaggc acacaagata
     1381 ttaacgatat gttggatact ttttttaatg agaaaggtaa gttaattggt atgttgcaaa
     1441 tgtttatgac aaaagagagc attgcagatc gaattcaaca agaacttata cgtttaacat
     1501 ctcaccctaa agcaagaaca attgtgacat cgttaattac caatgaatat caaactttta
     1561 aagataaacc attgaatgaa ctgttagatg cggcgcagtt taatgaaatc gctgaaaatc
     1621 tatcagtgta tgtaacaaca tatgctagca aacaagcgaa taagccagtg gttacgttaa
     1681 tgcctcaatt cgttgattat ttagaaggtc aactctctag caaattagca aatttaatca
     1741 ttgaacaatt atcgattcac ttatctacaa ttatgaaaaa agtggattta cgaggattaa
     1801 ttgaagaaca aattaatacg tttgacttag attatattga aaaattaatt attgaaattg
     1861 ctaacaaaga actaaagttg attatgtcat taggtttcat tttaggtggt attattggat
     1921 ttttccaagg tttggttgca atctttgtct aaaaaagcga gatgtggtaa agtaatatcg
     1981 aacgttttaa taaacatcac aacaaaggag tgctataacc atggcagtaa atttatatga
     2041 ttatgcaaat caattagaac aagctttaag agaaagcgaa gaatacaaag caatcaaaga
     2101 agcattcgct aatgtaaaag ctaacgaaga atctaaaaag ttattcgacg agttccgtga
     2161 aactcaaatt aacttccaac aaaaacaaat gcaaggtgaa gaaattgctg aagaagattt
     2221 acaaaaagcg caagaacaag cgcaagcaat tgaaaaagat gaaaacatct ctgcattaat
     2281 gaatgctgaa caaaaaatga gtcaagtatt ccaagaaatc aaccaaatta tcgttaaacc
     2341 attagacgaa atttacgctg actaatatta aaataatatt tttcaatcct ttaaatagag
     2401 gaatagactg ccgacagagt ccgagacttt gtgggtagtt tttttagttt tgataacgga
     2461 agttagaggc tctctgtcaa attgggcaga tgcgttcagt atcggaattt aggctagtaa
     2521 acgtgtccta acttcctttt tgcattttgg agtttaaagt tttcaacata atactattag
     2581 ttcggtcatg tatcggactg atgaaaaagt gtttcacttt taatgactca ttaagaacgg
     2641 cctgaaaatg tttgacgtat taagtgcaat gatagttttg acatttagct tctaattggt
     2701 cattactgcc gagcagaact agtagagtaa tcatgtaaat ctttaatgta tcagttggtt
     2761 caccagcaga gttaacagct acagaaattg aatttccgac tgaaattttt gaaaaaaatc
     2821 aacgcacgct acaaataaaa ttttaaactg ttataaatgt gtctcaattt catatgttca
     2881 tcgacgatat gaagcgtatt atggtaaaat gaagaaataa taaacttgtt aataaataaa
     2941 acatcacgat ttgactaaag cactttatta ttgtgtagat aatagttttt taacgaaata
     3001 aaaatgacga ctggttttaa taaatcagct aatgaatcac tacacctata agtatgaata
     3061 tagtgattag aatgctttgt atagttggat tttgcaaaat tgatgttaga acctttaatt
     3121 aaggagatat ggatgtatgg ttaaatttat tcattgttcg gatttacatt tagatagtcc
     3181 tttcaaatct aagagtcata taagccctaa aatttttgag gatgttcaaa aaagtgctta
     3241 tgaaagtttt aaaaatattg tagatattgc attacaacaa gatgttgatt ttgtaattat
     3301 cgcgggtgat ttatttgata gtgaaaacag aacattacgt gctgaaattt tcttaaaaca
     3361 gcaatttgaa cgtttacaaa atgaacaaat ctttgtttat gtttgtcatg gtaatcacga
     3421 ccctttatca tcaaagattt catcaaactg gccagataat gtttctgtat tttcaaataa
     3481 agttgagacg tatgaagcaa ttactaaatc gggtgaaaca atttatattc acggatttag
     3541 ttatgaaaat agagcaagtt atgagaacaa gattgatgaa tatccatcaa gtcaaggcca
     3601 aaaaggcata catattggtg tcttgcatgg tacgtacagt aaatcttcag ttaacgaaag
     3661 atataccgag ttcattttag aagatttaaa cagtaaattg tatcattatt gggctttagg
     3721 tcatatacat gaacgtcaac aattaagtga tatgcctgta attaactatt caggtaatat
     3781 tcaaggtaga cattttaatg agcaaggtga aaaaggttgc ttattaatcg agggtgacca
     3841 cttaaaatta aaaactaaat tttatcctac acagtatatt agatttgaag aagcaactat
     3901 tgaaacggat aagacatcta agcaaggttt atacgaggtc attcaaaact ttaaagaaca
     3961 agtgagagaa gaaggaaaag ccttttatcg tttaacgctt gttattaata gtgagacatt
     4021 aatttcacct caagatttat tacaagttga agaaatgatt acagattatg aagaaaacga
     4081 aaatcaattt gtatatattg atgagttaaa aatacaatat gcacaaaatg atgagtcacc
     4141 tttagttaat gaattttcag cggaattatt agtcgatcaa actgtttttg ataaagcgat
     4201 gtcagattta tatttaaatc caagggcatc taagttccta gacgattatg gaacattcga
     4261 ccatacagca ttagttaatc gtgctgaaga aatattaaaa gctgaaatga gaggtgaaca
     4321 aaatgataat taaatcactt gaaatttatg gttacggtca atttgttcaa cgtaaaattg
     4381 aatttaataa aaacttcact gaaatttttg gtgaaaatga agcgggtaaa tcgacgattc
     4441 aagcatttat ccattcgata ttatttggtt ttccaactaa aaagtctaaa gagccaagac
     4501 tagaaccacg tctaggtaac caatacggtg gtaaattagt acttattctt gatgatggct
     4561 tagagattga agttgaacga attaaaggca gtgctcaagg tgatgtgaaa gtatatttac
     4621 ctaatggtgc tgtgcgtgat gatgcttggt tacaaaagaa acttaattat atttctaaaa
     4681 agacatatca aggtatcttt tcatttgatg tactagggct tcaagatatt catagaaatc
     4741 taaatgaaaa acaattgcaa gattatttat tacaagcagg ggctttagga tcaactgaat
     4801 tcacgtcaat gcgcgaagtg attaatcgta aaaaagatga attatataaa aaatcaggta
     4861 aaaatccgat cattaatcaa caaattgagc aattaaaaca actagaaagt caaattcgtg
     4921 aagaagaagc taagctagaa acatatcatc gcttagtaga tgatcgtgat aaatcatcac
     4981 gtcgattaga gaatttaaaa cataatttaa atcaattatc aaaaatgcat gaagaaaaac
     5041 aaaaagaggt tgctttacat gatcattcac aagaatggaa gtctctagaa caacagttaa
     5101 atattgagcc aatcacattc ccagaaaaag gtgtagatcg ttacgaaaaa gcacgagcgc
     5161 ataagcaatc gttagaaaga gatattggtt taagaaatga gcgtttagcc caacttaaag
     5221 aagaagcgac tcaattagag ccagttaaac aatctgatat tgacgccttc attagtttga
     5281 atcaacaaga aaatgaaatt aaaaataaag aatttgaact tactgcaatc gaaaaggata
     5341 ttgcgaataa acaacgtgat aaagatgaat tgcaagcaaa tattggttgg tctgaaacgc
     5401 atcatgacgt agatagctct gaggcaatga aaagttatgt cagtgagcaa atcaagaata
     5461 aacaagaaca agcggcatac attaaacaat tagaacgtag tttagaagga aataaaatcg
     5521 aagataatgc ggttcatagc gaactagatt ctgttgaaaa aaaaatagtt cctgaagaga
     5581 cttttgaaaa gaaaaaagaa tactcacaac aagtcattga attaaacgaa aaagagaact
     5641 tgtatagcaa attgaaagaa cgtttcgaaa ttgagcaaca agaaaaacaa aaacgtcaaa
     5701 aattgttacg aacaacgttt attcttttaa cactagtagg tattggttta actgccttct
     5761 catttatttc aaacaacatg ttgtttggta ttattttcgc agtattaact ttagtatttg
     5821 ttatcggtat catcatgtct aaatcaaaag aagtagatta tagcgaggcc atcacagatg
     5881 aaatcgaaga aattaaagca caactagcca ttttagatga aaattatgac ttagattttg
     5941 atcttgatga gcaatatcgc attcgtgatc actggcaaca agctttgaaa aataaagata
     6001 ttttagaaga gaaacgtcag tatattgaag gtcgtttaaa tgatgcgaaa gggcgtcatg
     6061 atgaattaca atcaacagtt gaaaacgtta aggatgaatt atacttatca tctaaaattt
     6121 caaatgattt aattgttgat agtatttcta caatggcgaa tattaaagca ttagatcaac
     6181 atatcagtga tttaaatcaa caacgccagc aattggttca agaattagat acattctaca
     6241 atcatgctga agctgttaca aaatcacaat ttgtttattt caacaaatta tctttattcc
     6301 acgatgtgca acaatggttg aaatcagctg aagacacgaa tgaaaaatgg cgtattaatg
     6361 ctgaaaatac caaacttgtt acaaatgaat taaatcattt aaatgctcaa ttagaagaaa
     6421 ataataaaga aattacagca ctattcgatt ttatcaatgt tggtactgaa gaagattttt
     6481 accaacatca tgaagattac caaacttaca ctagtaattt gagtcgtttt aatgatttaa
     6541 ctaaatatct tgagaaccaa aactattctt atgaattaag ttctagttta agtgaaaaaa
     6601 caactgcaca acttgaagaa gaagatcatt tattggctac tcaagttgac gaatataatg
     6661 agcaatatct tgaaatgcaa gcacaagtca gtgatttaag tgcacaaatc aatcacatgg
     6721 aaactgatac aacgcttgct aatttgagac atgaatatca cagtcttaaa aatcaactta
     6781 atgatatcgc aaaagattgg gcaagtttaa gttatttaca aagtttagtt gatgaacaca
     6841 ttaaacaaat taaagataaa cgtttgcctc aagttattaa tgaagcggta gaaatattga
     6901 agcatttaac agatggcaga tatacgatga ttaactataa tgaagattca attacggtta
     6961 aacatgttaa tggtcaatta tatgatcctg ttgaactaag tcaatctaca aaagaattac
     7021 tttatgtagc tttacgtatc agtttaatta aggtactacg accatattat ccgttcccat
     7081 taattgttga tgatgcattt gttcattttg ataaaaaacg tactgaaaaa atgttgaatt
     7141 atttacgatc attatcagaa cactatcaag tactatactt tacatgtgta aaagataata
     7201 ttgttccatc aaaagaagtg attacattaa acaaaataga ggaaggcggg aaacgatgag
     7261 aaatatagag aatctaaatc ccggagattc agttgatcac tttttcttag tgcataaagc
     7321 tacacagggt gtaacagcac aaggtaaaga ttatatgaca ttacatttgc aagataaaag
     7381 tggtgaaatt gaagcgaaat tttggacggc tacaaaaaat gatatggcaa caatcaagcc
     7441 tgaagaaatt gtacatgtta aaggtgacat cataaactat cgcggaaata aacagatgaa
     7501 agtcaaccaa attagactag cgacaactga agatcaatta aaaacagaac aatttgtaga
     7561 tggtgcacct ttatcaccgg cagaaataca agaagagatt tctcattatt tgctagatat
     7621 tgaaaatgct aatttacaac gtattacacg tcatttattg aaaaaatatc aagaacgatt
     7681 ttacacatat ccagctgcta gttctcatca tcataacttt gcgagtggct taagctatca
     7741 tgtattaacg atgttacgta ttgcaaaatc aatttgtgac atttatccat tgttaaacaa
     7801 aagtttgtta tatagtggta ttattttgca tgatattggt aaagtcagag aattaagtgg
     7861 tcctgttgcg acgtcgtata cagtcgaagg taacttatta ggacacatct cgattgcgag
     7921 tgatgaagta gttgaagcag ctcgtgaatt gaacattgaa ggagaagaaa tcatgttgtt
     7981 aaggcatatg attttatctc atcatggtaa gttagagtat ggttcgccaa aactgccata
     8041 cttaaaagaa gcagaaattt tatgctatat cgataatatc gatgctagaa tgaatatgtt
     8101 tgaaaaagca tataaaaaaa ctgacaaggg tcagtttaca gataaaatat ttggtcttga
     8161 aaatcgtaga ttctacaatc ctgaatcact tgattaagaa ctaaaagata tcggacagat
     8221 gagtatactg aaacatgagc tcatctgtcc gatttttaat gcgaaaaaac cgagcggccg
     8281 tggtatgatt tttgaaccac ggtcgctcgg ttttgtgtta ttggctcatg ccggattgtc
     8341 cgccttgtgc gccaccttgt ttaagttttt cagggtttaa gattttatct tcgacaactg
     8401 atttaatatc acgatcttta aagtcaacat cgtattcttt taatagatct ttgtatgcat
     8461 cagttaataa ttttggattt ttttgtactt tctgatcgac taatttttct tttaggcttt
     8521 gtttttcact gttaaagtct gttggtttat cagctttaat aatatgatat ccaaaacttg
     8581 atttaacaac gtctgatact tcaccatctt taagtttaaa tagtgctttt tcaaaatctt
     8641 tatcagtttg tcctttaaga acataaccta attcgccatc ttttttagct gaaccagtat
     8701 ccattgattc ttttttagcg atttcaccaa atttacttgg atcttttgaa acttcttttt
     8761 gaatttcttc agctttttgt ttcgcttctt tatcatctaa gccttctttg tcgcttttct
     8821 tagatttaac tttaattaaa atgtgtgaag ctttcttgct gtcttcttta atttcagaat
     8881 cagagatttt aattttatct gatagtaatt ctttatgata agcagcagta cgtaaatttt
     8941 ctttatattt atcggctgtt aaaccttgct gttgaagggc cttttcaaat ttatctttac
     9001 cgccgtattg cttttgcatt ttttcaattt gctcgtcaat tttcttatcg ttaactttat
     9061 ttttatattt atcagctaaa attttattta acatttcagt aaacgatgca tttgcaattt
     9121 gatctttacc gatttttttc attgtatctg caactgttac atctccagct ttagaagaaa
     9181 ttaatgtatt ttctttagag tctgtggcac tagcgccaca agcgcctaat aataaagcac
     9241 tagctgttac tggaacgatt aatttgttta tcatcttcat agttgaaact cctttgtaag
     9301 ttaatcatcg tgaatataac atagttttat attatcacca atcaataacc catgacatca
     9361 gactagagca ttaggtttag gggtagaaat tgatgaaaat aaaaaaacaa ggtctatata
     9421 catttgattt taagctatta catagctgaa aaacaaataa taacaccttg ctataattat
     9481 tttgttataa atgatatg
//
LOCUS       DAHHOJ010000100         9190 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.100, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000100 DAHHOJ010000000
VERSION     DAHHOJ010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9190)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9190)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9190
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.100"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            370..1179
                     /locus_tag="NBH32_002118"
     CDS             370..1179
                     /locus_tag="NBH32_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1515697.1"
                     /translation="MCGLRSITLGTTNIEQTKHFMVDILGLNYEELLENSIRFGDADI
                     SPGTRLQFIQVPSEQLEESHFVGIGLRTPTDSGLEEYAEILSNKDIPFTTVKELNGNK
                     YFSLEDNNGHIFSIYSNENNYGVGLGMPSFESAVNPLHQVQGLGPVILKVNHVDITGQ
                     ILTNIFGLEVFAEYQPFDNADYHVQVFKVGTGGLGGEIHLMPVETEMTMPEYGAVDQV
                     EFETKDADFFNQAKSRLDEVEIPYQTLEQDDIESIRITENSGLSFIFTLQK"
     gene            1208..1411
                     /locus_tag="NBH32_002119"
     CDS             1208..1411
                     /locus_tag="NBH32_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499941.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515698.1"
                     /translation="MLHETWKERTPIKKVEVINTDAKKFTVSDMLTVGKQYDVINETE
                     EYYQIIDNSGLVGGYYKTYFKEV"
     gene            1592..2383
                     /locus_tag="NBH32_002120"
     CDS             1592..2383
                     /locus_tag="NBH32_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCD1515699.1"
                     /translation="MALKHYKNSDSTVFNDAKALFDLNKNILLKGPTGSGKTKLAETL
                     SEVVDTPMHQVNCSVDLDTESLLGFKTIKTNAEGQQEIVFVDGPVIKAMKEGHILYID
                     EINMAKPETLPVLNGVLDYRRQITNPYTGEVIKAVPGFNVIAAINEGYVGTLPMNEAL
                     KNRFVVIHVDYIDGDILKNVIKEQSLLQDDKQIEQIIKFNEDLRTMSKQGQISEEAAS
                     IRALLDLCDLITVMPVERAIKRTIIDKLEDEREQQAIYNAVELNF"
     gene            2397..4283
                     /locus_tag="NBH32_002121"
     CDS             2397..4283
                     /locus_tag="NBH32_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase activation protein NorD"
                     /protein_id="HCD1515700.1"
                     /translation="MSDRFIKFNDEQLDAKQVMMLQDLARLLLKNEQTQVKIQKFPYY
                     NPVQNVLITSWFWSHRPSHIEMAGLKTDVMLAAYGYHMMDVQIVNEVVQDKTFKHPKF
                     YQQLFKLLEDMRVLNSIKVERPSTAKLIDLRLDTRVSYTESQIKVYRTKTQYTDLLFL
                     YLEHAFLSQDFFDIPSIHSDLDDILVNMFLYLPNFFQNQNSEDNMYLAQRIMYQVDDI
                     LKEDMLNEYYYLPKTLYNTLASPEFDDLKRTDASQVYGQDDTSEDDDNESEKADSKSA
                     DSESKGGAYLEMELHEGQNSETLGNDEAREGDATDDMTDMMTKKGKGSNDTLNREEGD
                     AVGQSQAFQLDGVNKNVEIKWQIPEIEPQYVLEYQESKQDVQYEIKDLIQIIKKTIER
                     EQRDARFNLTKGRLQKDLINWFIDDQYKLFYKKQDLSKSFDATFTLLIDASASMHDKM
                     AETKKGVVLFHETLKALNIKHEILSFSEDAFDSDEHAQPNIINEIINYDYSTFEKDGP
                     RIMALEPQDDNRDGVAIRVASERLMRRNQHQRFLIVFSDGEPSAFNYSQDGIIDTYEA
                     VEMSRKFGIEVFNVFLSQDPITEDVEQTIHNIYGQYAIFVEGVAHLPGHLSPLLKKLL
                     LKSL"
     gene            4507..5850
                     /gene="brnQ"
                     /locus_tag="NBH32_002122"
     CDS             4507..5850
                     /gene="brnQ"
                     /locus_tag="NBH32_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HCD1515701.1"
                     /translation="MNKNTWVIGFTLFAMFFGAGNLIFPPNLGLDSGQFFWPAILAFV
                     LTGIGLPLLGVIVGALDKEGYIGALNKISPKFSILFLIIIYLTIGPLFAIPRTASTSF
                     EMTITPIIHSNSSIALFIFTIIYFIVVLYICLNPSKLIDRIGSLLTPLLLITILAMII
                     KGYLDFSGNSAGKGNEALYHSNFSSFAEGFTQGYLTMDAIAAIAFSMIVVNAVKLTGI
                     TKTNQIFKQTLTAGLIAAVALIFIYISLGYIGNHMPVSDMTLDQLKSKDRNIGTYLLT
                     TMASTGFGSFGKYLLGIIVALACLTTAYGLIVAVSEYFHRIVPKVSYKAFVLVFILMS
                     FIIANQGLNAVISMSIPVLSIVYPVAITVVLLILIAQFIPTKRITQQIPVIIVFILSI
                     FSVISKLGWLKINFIESLPLRAYSLEWFPVAIIATILGYLVGIFVKQDPIKYQQE"
     gene            complement(5923..7059)
                     /locus_tag="NBH32_002123"
     CDS             complement(5923..7059)
                     /locus_tag="NBH32_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HCD1515702.1"
                     /translation="MTENKSFKESHPLDDFISDKELSNNTIQKEKLTIEQQKQVDTIS
                     KQINPLDNEGLLAFGSDLQKQMSQFSHQMLDEVQSKDVGPIGDTLSDLMSKLKSVNPN
                     ELNTDKPSMLKRIFSRAKSSINEIFSRMQSVSAQVDRITIQLQKHQTHLTRDIELLDT
                     LYDKNKQYFDDLSLHIIAAQQKKLQLENEKLPQLQQQAQQSTNQMDIQQVADMQQFID
                     RLDKRIYDLQLSRQIALQTAPQIRMIQNVNQALAEKIQSSILTSIPLWKNQMAIALTL
                     MRQRNAVATQRAVTDTTNDLLTANAEMLKQNAIETATENERGIVDLDTLKRTQRNIIE
                     TIEETLIIQQHGREERQLAEKELQQLEQDLKSHLVNIKGPNKQS"
     gene            complement(7091..7720)
                     /locus_tag="NBH32_002124"
     CDS             complement(7091..7720)
                     /locus_tag="NBH32_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HCD1515703.1"
                     /translation="MTVRYNISHIFGVLVGIPVAFLTSIFGMIALDVSFLIDMSIGIV
                     GFLMTYLPIQKLTSRKYLNEIGLTRKDYRYIRNQLNHTHQKLRGILKTYVNIRSIKDF
                     RQINDIYQISRSIYTTVRQRPASFYKVEGFFYSHIDNALNLVDAYTRLAKMPKKSINE
                     QQKLEQTRITLDEVKRTLIADLKRLNEDDYERLDIEMELNKLHQKHHQD"
     gene            complement(7739..8008)
                     /locus_tag="NBH32_002125"
     CDS             complement(7739..8008)
                     /locus_tag="NBH32_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCD1515704.1"
                     /translation="MRHIHLQVFGRVQGVGFRYFTQRIAMNYNIVGTVQNVDDYVEIY
                     AQGDDADIERFIQGVIEGASPASNVTSHQLEELELNQKLSDFRSI"
     gene            8171..8479
                     /locus_tag="NBH32_002126"
     CDS             8171..8479
                     /locus_tag="NBH32_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HCD1515705.1"
                     /translation="MWTVTKIRADYEGWWLFSDWPENIVEKYQYQDFDDMFKHYQQLI
                     NQCKVQFDNYVTGKYNIYAFYNNCDMNYCEDCEEDLQIFYSFIVLQNNEVYYKLPIID
                     "
     gene            8650..8850
                     /gene="cspA"
                     /locus_tag="NBH32_002127"
     CDS             8650..8850
                     /gene="cspA"
                     /locus_tag="NBH32_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspA"
                     /protein_id="HCD1515706.1"
                     /translation="MKQGTVKWFNAEKGFGFIEVEGENDVFVHFSAINQDGYKSLEEG
                     QAVEFEVVEGDRGPQAANVVKL"
     gene            9047..>9190
                     /gene="msa"
                     /locus_tag="NBH32_002128"
     CDS             9047..>9190
                     /gene="msa"
                     /locus_tag="NBH32_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarA expression modulator protein Msa"
                     /protein_id="HCD1515707.1"
                     /translation="MKYLILSLVANLLVFGVLSAIGLNINILAAMMIVLVIPIMISGI
                     LFFK"
BASE COUNT         3150 a         1308 c         1532 g         3200 t
ORIGIN      
        1 tttcaatatg ctaatgaaat cattttagat aataacaagc gatgaaattt atgaattcta
       61 taagcgcctt tagttaattt aaaatcatct gaacaagatg taaattcact ttatggatac
      121 gacatgtata tgttttttat gtactacata aaacgaaata ttcaattgct atatacagat
      181 actcattaat gacatactca atagcaacca taacaatatt atgttaccta tttaaatgaa
      241 atagttcttt taaagaaata catttttcac atattaatct ataatcaaaa tcaactgacc
      301 gatattctat aatttatgat taaaataagt tataatataa tagtaaagat aaagatagag
      361 gtggctataa tgtgtggact tagaagtata acattaggta caacaaatat agaacagaca
      421 aaacatttca tggttgacat attaggatta aattatgaag aacttcttga aaactcaatt
      481 cgtttcggcg atgcagatat aagcccagga acaagacttc aatttataca agttccaagt
      541 gagcaattag aagaatctca ctttgtgggt attggattac gtacaccaac tgactcaggt
      601 ttagaggagt atgcggaaat attatcgaat aaggatattc catttacaac agttaaagaa
      661 ttaaatggca ataaatattt cagtctagaa gataacaatg gtcatatttt ctcaatatat
      721 tcaaacgaga ataattatgg cgttggttta ggtatgcctt cttttgagag tgcggtcaat
      781 ccgttacatc aagtgcaagg tttaggacca gtgattctta aagtgaatca tgtagatatt
      841 acaggtcaaa ttttaacaaa tatattcgga cttgaagtat ttgcagaata ccaacccttc
      901 gacaatgctg actatcatgt ccaagtattc aaagttggaa cgggtggtct aggtggcgaa
      961 atacatttaa tgcctgtaga aaccgaaatg acaatgccgg aatatggcgc agtcgatcaa
     1021 gttgagtttg aaacgaaaga tgcagatttc tttaatcaag cgaaatcgcg cttagatgaa
     1081 gtggaaatac catatcaaac gcttgagcaa gatgatattg aatcaattag aattactgaa
     1141 aacagtggat tatcgtttat attcacttta caaaaataat ttttttacga tagtgaggac
     1201 aaaatttatg ttacatgaaa cttggaaaga acgtacacca atcaagaaag tagaagtcat
     1261 taatacagat gcaaagaaat ttacagtttc tgacatgtta acggttggca aacaatatga
     1321 tgttatcaac gaaactgaag aatattatca aataatagat aattctggat tagttggcgg
     1381 ttactataaa acatatttca aagaagttta aacgcataat tattgaaatg aagcctaata
     1441 tgagattttc aattagtaaa tttggtaaaa tataaacaat tcgcatacta agcattgcgt
     1501 tttatatcag aatagaatag taaactaagg catttggaga acttattcca tgtcttagtt
     1561 ttttaattta tttaaaggag aacgttttaa aatggcacta aaacattata agaattcaga
     1621 ttcaacagtt ttcaatgatg cgaaggcatt atttgattta aataaaaata ttttacttaa
     1681 aggtccaacg ggttcaggga aaacgaagtt ggcagaaaca ttaagtgaag ttgttgatac
     1741 acccatgcat caagtcaact gttctgttga tttagataca gaaagcttat taggctttaa
     1801 aacaattaaa acaaatgcgg aaggacaaca agaaattgtc tttgtagatg gtccagttat
     1861 taaagctatg aaagaggggc atattttata tattgatgaa ataaatatgg ctaaacctga
     1921 aacattgcct gtattaaatg gggtcttaga ttatcgtcgt caaattacga atccatacac
     1981 tggtgaagta atcaaagctg taccaggatt taacgttata gcagcgataa atgaaggtta
     2041 tgttggtaca ttgccaatga atgaagcact aaaaaatcgc tttgttgtta ttcacgttga
     2101 ctatattgat ggggacattt taaaaaatgt gattaaagag caaagtttat tacaagatga
     2161 taaacaaatc gaacaaatta ttaagtttaa cgaagattta cgtactatgt ctaagcaggg
     2221 acaaatttct gaagaagccg ctagtatccg tgcattatta gacttgtgtg atttaatcac
     2281 tgtaatgcca gttgaacgtg caattaaacg tacaataatt gataaattgg aagatgaacg
     2341 tgaacaacaa gcaatatata atgctgtaga actaaacttt taaagaggga taaaacatga
     2401 gtgatcgttt cataaaattt aacgacgaac agttagatgc aaagcaagta atgatgttgc
     2461 aagacttagc tcgactctta ttaaaaaatg aacaaacaca agttaaaatc caaaaattcc
     2521 cttactataa tcctgttcaa aatgttttga ttacgagttg gttttggtca catcgaccaa
     2581 gccatatcga aatggctggt ttaaaaacag atgttatgtt agctgcttac ggttatcata
     2641 tgatggatgt acaaattgta aatgaagtag tacaagataa aacttttaaa caccctaaat
     2701 tttatcaaca actttttaaa ttgttagaag atatgcgtgt attaaattct attaaagttg
     2761 agcgcccaag tactgctaaa ttaattgatc ttcgcttaga tacacgggta tcatatacgg
     2821 aatctcaaat taaagtgtat cgtactaaaa cacaatatac agatttatta tttttgtatt
     2881 tagaacatgc atttttaagt caagatttct ttgatatacc atctatacat tcagatttag
     2941 atgatatttt agtaaatatg ttcttatatt tacctaattt tttccaaaat caaaattctg
     3001 aagataatat gtatctggct caaagaatta tgtatcaagt agatgatatt ttaaaagaag
     3061 atatgctaaa cgaatattat tacttaccta aaacactata taatacatta gctagtccag
     3121 agtttgatga tttaaagaga actgatgcaa gtcaagtcta tgggcaagat gatacttcag
     3181 aagatgatga taacgaaagt gaaaaagctg actctaaatc tgcagatagt gagtcaaaag
     3241 gtggcgccta tttagaaatg gagcttcacg aaggacaaaa tagtgaaaca ttaggtaatg
     3301 atgaagcacg tgaaggcgat gctacagatg atatgactga tatgatgacc aaaaagggca
     3361 aaggatcaaa cgatacgcta aatcgtgaag aaggcgatgc tgttggtcaa agccaagcat
     3421 ttcaacttga tggtgtgaac aaaaatgtag aaataaaatg gcaaattcct gaaattgagc
     3481 cacaatatgt tttagaatat caagaatcga aacaagacgt acaatatgaa atcaaagact
     3541 taattcaaat tataaagaaa acgattgaac gagaacaacg agatgcacgt tttaatttaa
     3601 caaaagggcg attacaaaag gatttaatta attggtttat cgacgatcaa tataaattgt
     3661 tttacaaaaa acaagattta agtaaatcat ttgatgcgac atttacattg ttaattgatg
     3721 cgtcagcaag tatgcatgat aaaatggctg aaacgaagaa aggtgtagtg ttattccacg
     3781 agacgttaaa agcattgaac attaaacatg aaattttatc attcagtgag gatgcctttg
     3841 attcagacga gcatgcacaa ccaaatatca ttaatgaaat tattaactat gattactcaa
     3901 cctttgaaaa agatggccca cgtattatgg cacttgaacc tcaagatgat aatcgtgatg
     3961 gtgttgctat tagagttgca agtgaacgat taatgcgtcg aaatcaacat caacgatttt
     4021 taatcgtatt ttcagacggc gaaccgtctg catttaatta tagtcaagat ggtattattg
     4081 atacgtatga agctgtagaa atgtcacgta aatttggtat tgaagtattt aatgtatttt
     4141 tgagtcaaga tccaataact gaagatgttg aacaaacaat tcataatatt tatggtcaat
     4201 atgctatttt cgttgaaggt gttgcgcatt tacctggaca tctctctcca ttattaaaaa
     4261 aattactact taaatcttta taaataaatt taaaatatga gtttatatga aattgtgaat
     4321 aaaagtcatc agttactatt taaaaagtgg atttgaagaa ttttattttc tgacataatt
     4381 tgatttaaac aacacatgtg ttttgtttca gctatgtaca acatatttca tatagactct
     4441 ttattatttt ttgtgctaaa atatttactt gtaaattatt tcgtgtttta aaaaaggagt
     4501 aactctatga ataaaaatac atgggtcatt gggtttacgc tcttcgctat gttttttggc
     4561 gcaggcaatc taatttttcc tcctaatctc ggattggata gtggtcaatt tttctggccc
     4621 gccattttag cgtttgttct aactgggatt ggtttaccat tattaggtgt gattgtaggt
     4681 gcacttgata aagaaggata tattggcgca ttaaataaaa tttcacctaa attttcaata
     4741 ttgttcttaa tcatcattta tttgactata ggaccacttt tcgcaatacc tagaactgca
     4801 tctacatctt ttgaaatgac aattacacca attatacata gcaatagtag tatcgcttta
     4861 tttatattta cgattatcta cttcatagtc gttttgtata tttgtttaaa tccatctaag
     4921 ttaatcgatc gtattggttc attattaaca ccattattat tgattactat tttagcgatg
     4981 attattaaag gatacttaga ctttagcggt aatagtgctg gaaagggcaa tgaagcacta
     5041 tatcattcta atttttcaag ttttgctgaa ggctttacac aaggctattt aacaatggat
     5101 gccattgcag caattgcttt ttcaatgatt gttgttaatg cagtaaaact aactggtatt
     5161 actaaaacaa atcaaatatt caaacaaact ttgactgctg gtttaattgc agccgtagct
     5221 ttaattttca tatatatttc attaggttat attggtaatc atatgccagt aagtgacatg
     5281 acgttagatc aattgaaatc caaagatcga aacattggga catatttatt aacgacaatg
     5341 gcttcaacag gatttggttc attcggaaaa tatttattgg gcatcattgt ggcgctggca
     5401 tgtctaacta cagcatatgg gcttattgtt gcagtttctg aatatttcca tagaatcgta
     5461 cctaaagtat catacaaagc atttgtatta gttttcattt taatgagttt tattattgct
     5521 aaccaaggtt taaatgccgt tatctcaatg tcaattcctg tattaagcat tgtatatcca
     5581 gtagcaataa ctgttgtatt attaatttta attgcccaat tcataccgac aaaacgcatt
     5641 acacaacaaa ttccagttat tatcgtattt atattgtcga ttttcagtgt tattagtaag
     5701 ttaggttggc tgaaaattaa ctttatagaa tcattgcctc taagagcgta ttctttagag
     5761 tggttcccag tagcaattat tgcaacgata ttaggctatc tagtcggcat atttgtaaaa
     5821 caagatccaa ttaaatatca acaggaataa cgaataatat aaaaggaagc atctccaatt
     5881 taatcgtagg atgcttcctt tttgttatat ctaattcgta atttatgatt gtttattcgg
     5941 tcctttgatg ttcactaaat gtgactttaa atcttgttct aattgttgta attctttttc
     6001 agctaattgt cgttcttcgc gaccgtgttg ttgaataatt aatgtttctt caattgtctc
     6061 aataatgtta cgctgtgtac gtttcaatgt atcaagatca acaatgccac gctcattttc
     6121 tgttgcagtt tcaatcgcat tttgtttcaa catttcagca tttgctgtta ataaatcatt
     6181 agttgtatca gtgacagctc gttgtgtagc aactgcatta cgctgtctca ttaatgtaag
     6241 cgcaatggcc atttgatttt tccatagtgg aatacttgtc aaaattgaac tttgtatctt
     6301 ctcggcaagt gcttgattaa cattttgaat catacgaatt tgtggcgcag tttgcaaagc
     6361 tatttgtctt gaaagctgta agtcatatat gcgtttatct agtctatcta taaattgctg
     6421 catatctgca acttgttgaa tatccatttg attagtggat tgctgcgctt gctgttgcaa
     6481 ttgtggtagc ttttcatttt ctaattgcaa ctttttttgc tgtgcagcaa tgatatgcaa
     6541 tgataagtca tcaaagtatt gtttgttttt atcatatagc gtatctaata attcaatatc
     6601 tcttgttaaa tgcgtttgat gtttctgcag ttgaattgtt atgcgatcga cttgagcact
     6661 aactgattgc attcttgaaa agatttcatt gatagacgac tttgctctgc taaaaattct
     6721 ttttaacata gatggtttat cagtatttaa ctcatttgga ttaactgact ttagttttga
     6781 cattaaatct gacaaagtat ctccaatagg accaacatct ttactttgta cttcatccaa
     6841 catttgatgt gaaaattgag acatttgttt ctgtaaatca gaaccaaacg ccaataaacc
     6901 ttcattgtct aaagggttaa tttgtttact gattgtgtct acctgttttt gttgttcaat
     6961 tgttaatttt tctttttgaa tagtattatt cgataattct ttatcgctta taaaatcatc
     7021 tagtggatga ctttctttga aacttttatt ttcagtcatc aatcatctct ccaatagttg
     7081 ctattttaca tcaatcttga tgatgttttt gatgtaactt atttaattcc atttcaatat
     7141 ctaaacgttc ataatcatct tcgttgagac gctttaaatc agcgattaat gttcgtttga
     7201 cctcatccaa agtaattcgt gtttgttcta acttttgctg ttcattaatt gattttttgg
     7261 gcatttttgc tagacgtgta tatgcatcaa ccaaatttaa agcattatca atatgagaat
     7321 aaaaaaagcc ttcaacttta taaaatgatg caggtctctg tctaactgtc gtataaatag
     7381 aacgtgaaat ttggtatata tcattaatct gcctaaaatc tttaattgat cttatattga
     7441 catacgtttt taaaatacct ctaagttttt gatgtgtatg atttaactga tttcgaatat
     7501 agcgatagtc ttttctagtc aaaccaattt cgtttaaata tttgcgtgaa gtgagttttt
     7561 gtatcggtag gtatgtcatt aaaaagccaa caataccaat agacatatca attaaaaaag
     7621 atacatcaag tgcaatcatc ccaaatatgc ttgttaaaaa cgctacagga attcccacta
     7681 acaccccaaa tatatgagaa atattatatc tcactgtcat cttcctttat ttagcatttt
     7741 atattgatcg aaaatccgat aatttttgat ttagttctaa ctcttcaagt tgatggcttg
     7801 ttacatttga tgctggtgag gcaccttcaa ttacaccttg aataaatctc tctatatctg
     7861 cgtcatcccc ttgtgcatat atctctacat agtcatctac attttgaaca gtaccgacaa
     7921 tgttatagtt cattgcaatg cgttgtgtaa aatatctaaa tccgacgcct tgaacgcgtc
     7981 cgaatacttg taaatgtata tgtctcattt ttaccacctc ataatgttat tatacgtagt
     8041 tttacttaaa aaaactaata attactatag ttactacttt gtttgtttca agtcgtcaaa
     8101 cttgattttc agaggataaa ggtataaaaa taagtataga gtttttgaag tatggaaggg
     8161 gtctttaata atgtggacag ttaccaaaat tagagccgat tatgagggat ggtggttatt
     8221 cagtgactgg ccagaaaaca ttgttgaaaa atatcaatat caagattttg atgacatgtt
     8281 taagcactat caacaattga ttaatcaatg taaagttcag ttcgataatt atgtcacagg
     8341 caaatataat atttatgcat tttataataa ttgtgacatg aactattgcg aagattgtga
     8401 agaggattta caaatatttt atagtttcat tgttttacaa aataatgaag tatattataa
     8461 actaccaata attgattaaa tgaatatttt ttattattgt attgcaattt ggtaataact
     8521 gcactataat acaagttaag cagatgatta ttccatattg caaagaatat ttgagtgaaa
     8581 tttgcatgta ttttttgtaa tatgcgaata agcatattga atgaatttta gtcttggagg
     8641 tttcagatta tgaaacaagg tacagttaaa tggtttaacg ctgaaaaagg attcggcttt
     8701 atcgaagttg aaggagaaaa tgacgtattc gtacattttt cagcaattaa ccaagatggt
     8761 tacaaatcat tagaagaagg tcaagctgtt gagtttgaag tagttgaagg cgaccgcggt
     8821 ccacaagccg caaacgttgt taaactataa ttcttagatt tgaatcattg attttaacta
     8881 acaccttacc aagaagtaag gtgtttttat atgcttatta caaatactta acgcgatgag
     8941 tttctcaacc aaattgagaa ctcttttttt atatcaccaa cattgtttgc aatagaaaat
     9001 tatatttata taatataatt atggtgtaca acgacgaggt gattttatga aatatcttat
     9061 ccttagttta gttgctaatt tattagtttt cggtgtatta tctgcaattg gtttaaatat
     9121 taatatttta gcagcaatga tgattgtatt agtgattccg ataatgattt ctgggatatt
     9181 atttttcaaa
//
LOCUS       DAHHOJ010000101         9160 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.101, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000101 DAHHOJ010000000
VERSION     DAHHOJ010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9160)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9160)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9160
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.101"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            215..1225
                     /gene="adhP"
                     /locus_tag="NBH32_002129"
     CDS             215..1225
                     /gene="adhP"
                     /locus_tag="NBH32_002129"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCD1515708.1"
                     /translation="MRAAVVTKDHKVSIEDKKIRALKPGEALVQTEYCGVCHTDLHVK
                     NADFGDVTGVTLGHEGIGKVIEVAEDVESLKIGDRVSIAWMFESCGRCEYCTTGRETL
                     CRSVKNAGYTVDGAMAEQVIVTADYAVKVPEKLDPAAASSITCAGVTTYKAVKVSNVK
                     PGQWLGVFGIGGLGNLALQYAKNVMGAKIVAFDINDDKLAFAKELGADAIINSKDVDP
                     VAEVMKLTDNKGLDATVVTSVAKTPFNQAVDVVKAGARVVAVGLPVDKMNLDIPRLVL
                     DGIEVVGSLVGTRQDLREAFEFAAENKVTPKVQLRKLEEINDIFEEMENGTITGRMVI
                     KF"
     gene            1302..1409
                     /locus_tag="NBH32_002130"
     CDS             1302..1409
                     /locus_tag="NBH32_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515709.1"
                     /translation="MKDSENLVFFGFEVLFVFYKMAYIYEALIKYGIVN"
     gene            1502..1918
                     /locus_tag="NBH32_002131"
     CDS             1502..1918
                     /locus_tag="NBH32_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000359087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HCD1515710.1"
                     /translation="MDKKVSIQTKQVLKQHNEKEKFEFTTEGTWQQRQSNFIRYVEQI
                     EDATVNVTIKVDDDSVKLIRKGDINMNLHFVEGQTTTTFYDISAGRIPLEVKTLRILH
                     FVSGDGGKLKIHYELYQDNEKMGSYQYEINYKEIGE"
     gene            1915..3576
                     /locus_tag="NBH32_002132"
     CDS             1915..3576
                     /locus_tag="NBH32_002132"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCD1515711.1"
                     /translation="MNIIDQVKQTLVEEIAASINKAGLADEIPDIKIEVPKDTKNGDY
                     ATNIAMVLTKIAKRNPREIAQAIVDNLDTEKAHVKQIDIAGPGFINFYLDNQYLTAII
                     PEAIEKGDQFGHVNESKGQNVLLEYVSANPTGDLHIGHARNAAVGDALANILTAAGYN
                     VTREYYINDAGNQITNLARSIETRFFEALGDNSYSMPEDGYNGKDIIEIGKDLAEKHP
                     EIKDYSEEARLKEFRKLGVEYEMAKLKNDLAEFNTHFDNWFSETSLYEKGEILEVLAK
                     MKELGYTYEADGATWLRTTDFKDDKDRVLIKNDGTYTYFLPDIAYHFDKVKRGNDILI
                     DLFGADHHGYINRLKASLETFGVDSNRLEIQIMQMVRLMENGKEVKMSKRTGNAITLR
                     EIMDEVGVDAARYFLTMRSPDSHFDFDMELAKEQSQDNPVYYAQYAHARICSILKQAK
                     EQGIEVTAANDFTTITNEKAIELLKKVADFEPTIESAAEHRSAHRITNYIQDLASHFH
                     KFYNAEKVLTDDIEKTKAHVAMIEAVRITLKNALAMVGVSAPESM"
     gene            3910..4545
                     /locus_tag="NBH32_002133"
     CDS             3910..4545
                     /locus_tag="NBH32_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III domain-containing protein"
                     /protein_id="HCD1515712.1"
                     /translation="MLGTDELYKLLYRHMGPQNWWPADNDIEMMLGAILVQNTRWRNA
                     EIALNQIKEHTHFNPNHILELSIETLQSLIHSSGFYKSKSLTIKTLLTWLARHHFNYQ
                     EINERYKGELRKELLSLKGIGSETADVLLVYIFGRIVFIPDSYTRKIYDKLGYENTKS
                     YDQLKKVVTLPNHFTNQDANEFHALLDVFGKHYFRDKDIKNYDFLEPYFKK"
     gene            4918..5805
                     /locus_tag="NBH32_002134"
     CDS             4918..5805
                     /locus_tag="NBH32_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1515713.1"
                     /translation="MKKSLIAFILIFMLVLSGCGMKDNHKQGSDDNGSSKSPYHRIVS
                     LMPSNTEILYELGLGKYIVGVSTVDDYPKDVKKGKKQFDALNLNKEELLKAKPDLILA
                     HESQKATANKVLSSLEKQGIKVVYVKDAQSIDETYNTFKQIGKLTHHDKQAEQLVEET
                     KDNIDKVIDSIPAHHKKSKVFIEVSSKPEIYTAGKHTFFNDMLEKLEAQNVYSDINGW
                     NPVTKESIIKKNPDILISTEAKTRSDYMDIIKKRGGFNKINAVKNTRIEVVNGDEVSR
                     PGPRIDEGLKELRDAIYRK"
     gene            5954..6904
                     /locus_tag="NBH32_002135"
     CDS             5954..6904
                     /locus_tag="NBH32_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1515714.1"
                     /translation="MTFNKVLLSWIVILIITTSIYLFWQLGDINDVFNQSILINVRLP
                     RLLEALLTGMILTVAGLIFQTVLNNALADSFTLGLASGATFGSGLALFLGLTTLWIPV
                     FSITFSLITLITVLVITSVLSQGYPVRILILSGLMIGALFNSLLYFLILLKPRKLNTI
                     ANYLFGGFGDAEYSNVSIIAITFIIALFGIFIILNQLKLLQLGELKSQSLGLNVQLIT
                     YIALCIASMITAINVAYVGIIGFIGMVIPQLIRKWQWKQSLGRQLALNIVIGGQIMVM
                     ADFIGSHILSPVQIPASIIIALIGIPVLFYMLISQSKRLH"
     gene            6959..7678
                     /locus_tag="NBH32_002136"
     CDS             6959..7678
                     /locus_tag="NBH32_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1515715.1"
                     /translation="MDLNQIKAVVFDLEGTLLDRVKSREKFIEEQYERFHDYLIHVQL
                     ADFKKAFIELDDDEDNDKPDLYKEIIKRFHVDRLTWKDLFNDFEMHFYRYVFPYYDTL
                     YTLEKLSQKGFQIGVIANGKSKIKQFRLHSLGLMHVINYLSTSETVGFRKPHPKIFED
                     MIDQLGVLPEQIMYVGDDALNDVAPARAMGMVSVWYKQEDAEIEPLEEEVDFTITTVE
                     ELLTILPIKNDNKGENYGSIY"
     gene            7662..8462
                     /locus_tag="NBH32_002137"
     CDS             7662..8462
                     /locus_tag="NBH32_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1515716.1"
                     /translation="MDLFTRKDGTSIHYSTLGEGYPIVLIHTVLDNYSVFNKLAAELA
                     KSFQVVLIDLRGHGYSDKPRHIEIKDFSDDIVELLKYLYIEEVAFVCHEMGGIIGADI
                     SVRYPEFTSSLTLVNPTSIEGELPEERLFRKYAHIIRNWDPEKQDKFLNKRKYYRPRK
                     MNRFLKHVVDTNEISTKEEIQAVKEVFKNADISQTYRNVVVPTKIIAGEFGERTTRLE
                     AKEVADLIQNADFEVYQESSAFPFVEEQERFVEDTASFINKHHDEKHV"
     gene            8598..9104
                     /locus_tag="NBH32_002138"
     CDS             8598..9104
                     /locus_tag="NBH32_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515717.1"
                     /translation="MKKLLTASIIACSVVMGVGLVNTSAEAASGNSIDTVKQLIKGDQ
                     SLENVKIGESIKDVLTKYKNPMYSYNEDGTEHYYEFHTKKGMLLVTTDGKKNNGKVTH
                     ISMMYNDANGPTYQAVKNYVGKAVTHTEYSKVAGNFGYIEKGKTTYQFASAPKDKNIK
                     LYRIDLEK"
BASE COUNT         3347 a         1185 c         1771 g         2857 t
ORIGIN      
        1 gcatatcgat tatgtcattt cttttaacat attaactagg gaaacgttaa aagttaacgg
       61 ttgatatcta actaaaaaca aggtcacagt agtatgtttt aatctggcgt ctattacaaa
      121 taaaaattac atctataatt attcgttttc ttttttgaaa gtaatagcca attaatatca
      181 tacatactgg agtgactata aggaggacat tattatgaga gcagcagttg taacgaaaga
      241 tcacaaagta agtattgagg acaaaaagat aagagcttta aaacctggtg aagcgttggt
      301 acaaacggaa tattgtggcg tttgtcatac cgatttacat gttaagaatg ctgattttgg
      361 tgatgttaca ggcgttactt taggtcatga aggtattggt aaagtcatcg aagttgcgga
      421 agatgtagaa tcattaaaaa ttggagaccg tgtgtctatc gcttggatgt tcgaaagctg
      481 tggaagatgt gaatattgta caacaggtcg tgaaacactt tgccgtagtg tgaaaaatgc
      541 tggttataca gtagatggtg caatggctga acaagttatt gttactgcag actatgctgt
      601 gaaagtacct gaaaaattag atccagcagc agcgtcttct attacatgcg caggtgtgac
      661 aacttataaa gctgtaaaag taagtaatgt aaaacctgga caatggttag gtgtttttgg
      721 tataggtggt ttaggtaacc tagctttaca atatgctaaa aacgttatgg gggctaaaat
      781 tgttgccttc gacatcaatg atgataaatt agcattcgcg aaagaattag gtgctgatgc
      841 tattattaat tctaaagatg ttgatccagt tgcagaagtt atgaaattaa ctgataacaa
      901 aggattagat gcaacagtgg taacttcagt tgctaagacg ccatttaacc aagcggttga
      961 tgttgtaaaa gctggtgcaa gagttgttgc tgttggttta cctgttgata aaatgaactt
     1021 agatatccca agattagtgc ttgacggtat tgaagtagta ggttcacttg ttggtacaag
     1081 acaagactta cgtgaagcgt ttgaatttgc tgctgaaaat aaagtaacac ctaaagttca
     1141 attaagaaaa ttagaagaaa tcaatgatat ttttgaagaa atggaaaatg gtactataac
     1201 tggtagaatg gttattaaat tttaaaaata ttaattgatt aaatatagat gaagaaggta
     1261 gtgctctgaa cactatcatt ataatcaaac acgaggtttt catgaaagat agtgaaaatc
     1321 tcgtgttttt tggttttgag gtgttgtttg tattttataa aatggcttac atatatgaag
     1381 cgttgattaa gtatggaatt gttaattaat tgaacctatt tagctttaag aaggcataac
     1441 aagatgacct tattttatgc tataatattt ctattatgcg aagattaagg tgagtagtaa
     1501 attggataaa aaagtaagta ttcaaacaaa gcaagtgttg aaacagcaca acgaaaaaga
     1561 aaaatttgaa tttactactg aaggaacttg gcaacaaagg caatctaact ttattcggta
     1621 tgtagaacaa attgaggatg caacagttaa tgttacaata aaagtggatg atgatagcgt
     1681 taagttgatt cgtaaaggcg acattaatat gaatttgcat tttgttgaag gacaaacgac
     1741 aacaactttt tacgatatat cggctggacg aattccacta gaagttaaaa cattacgcat
     1801 tttacatttc gtaagtggag acggtggcaa gctaaagatt cattatgaat tatatcaaga
     1861 taatgaaaaa atgggttctt atcaatatga aattaactat aaggagatag gcgaatgaat
     1921 attattgatc aagtgaaaca aacattagta gaagaaattg cagcaagtat taacaaagca
     1981 ggattagcag atgagattcc tgatattaaa attgaagttc ctaaagatac aaaaaatgga
     2041 gattatgcta ctaatattgc gatggtactg actaagattg caaagcgtaa tcctcgtgaa
     2101 attgctcaag cgattgttga taacttagat actgaaaaag cacatgtaaa acaaattgac
     2161 attgctggtc caggattcat taatttttac ttagataatc agtatttaac agcaattatt
     2221 cctgaagcaa ttgaaaaagg tgatcaattt ggacatgtaa atgaatcaaa agggcaaaat
     2281 gtattgcttg agtatgtttc agctaaccct acaggagatt tacatattgg tcatgctaga
     2341 aatgcagcag ttggtgatgc tttagctaat attttaactg cagctggcta taatgtaaca
     2401 cgtgaatatt atattaatga tgctggtaat caaattacta acttagcgcg ttcgattgaa
     2461 acacgtttct ttgaagcttt aggtgacaat agttattcaa tgccagaaga tggctataat
     2521 ggaaaagata ttattgaaat aggtaaagat ttagcagaga aacaccctga aattaaagat
     2581 tattctgaag aagcacgttt gaaagaattt agaaaattag gcgtagaata cgaaatggct
     2641 aaattgaaaa atgatttagc agagttcaat acgcattttg ataattggtt tagtgaaaca
     2701 tctttatatg aaaaaggcga aattcttgaa gttttagcaa aaatgaaaga attaggttat
     2761 acgtatgaag ctgatggcgc tacatggtta cgtacaactg attttaaaga cgacaaagac
     2821 agagtattaa ttaaaaatga cggtacatat acgtatttct taccagatat tgcgtaccac
     2881 ttcgataaag ttaaacgtgg taatgacatt ttaatcgatt tatttggtgc tgatcatcat
     2941 ggttatatta atcgtttgaa agcatctctt gaaacgtttg gtgtagatag taatcgttta
     3001 gaaattcaaa tcatgcaaat ggttcgttta atggaaaatg gtaaagaagt gaagatgagt
     3061 aaacgtactg gtaatgccat tacattaaga gaaattatgg acgaagttgg cgttgacgct
     3121 gcacgttatt tcttaactat gcgtagtcct gatagtcact ttgattttga tatggaatta
     3181 gcgaaagagc aatctcaaga taacccagtt tattatgctc aatatgctca cgcacgtatt
     3241 tgttcaattt taaaacaagc aaaagagcaa ggtattgaag tgactgctgc gaatgatttt
     3301 acaacgatta ctaatgaaaa agcgattgaa ttgttgaaaa aagtagctga tttcgaacct
     3361 acaattgaaa gtgctgctga gcatagatcg gcacatagaa ttactaatta tattcaagat
     3421 ttagcttctc atttccataa attctataat gctgaaaaag tgttaacaga tgatattgaa
     3481 aaaacaaaag cacatgttgc tatgattgaa gcggttagaa ttacattgaa aaatgcattg
     3541 gcaatggtcg gtgtaagcgc acctgaatca atgtaagaac atttatatac actccaacgt
     3601 agagtttctc gaaagatact ttgtgttgga gtgtttttaa ttaggtatgt gacatattgg
     3661 ggaatgctta gtatgtgaat aaggttaaga gaacgcagtt ggcttgcttg cgcacaactg
     3721 cataagagag cctgagacat aaattaatgt tctatgctct acaaagttat aatggcagta
     3781 gttgactgaa cgaaaattcg cttgtaacaa gcttttttca attctagtca accttgccgg
     3841 ggtgggacga cgaaataaat tttacgataa tatcatttct gtcccactcc ctctaaaagg
     3901 aggatgtaaa tgttaggaac tgatgaattg tataaattgt tatataggca tatgggaccg
     3961 caaaattggt ggcctgctga taatgacatt gaaatgatgt taggtgcaat tttagttcaa
     4021 aatactagat ggcgaaatgc agaaattgca ttgaatcaga ttaaagaaca cacgcatttt
     4081 aatccaaatc atatattaga actatctatt gaaacgttac aatcattgat acattcaagt
     4141 ggtttttata aaagtaaatc actgacgatt aaaacattat taacatggtt agcacgacat
     4201 catttcaatt atcaagagat taatgagcga tataaaggtg aattaagaaa agaattatta
     4261 tctttgaaag gtattggaag tgaaacagca gatgtcttac ttgtttatat attcggacgc
     4321 attgtattta ttccagatag ctatacaaga aaaatatatg ataagttagg atatgaaaac
     4381 actaaaagtt atgatcaatt aaaaaaagta gtcacattac caaatcattt tacaaatcaa
     4441 gatgctaatg aatttcatgc tctgttagat gtatttggta aacattactt tagagacaaa
     4501 gatataaaga attatgattt tttagaacct tactttaaaa agtaaacgct gtgaagttag
     4561 atagatgagt ttatatgaaa tataaaaaat aatttactat tttcttttag tatgtggact
     4621 tatataataa atagaagcat ataaagtgaa aaacagttgg atgcttgggc acaactgcat
     4681 aaatccctct aatcgctaaa ggtgaagagg aacgcagttg gatgctaccg cgcaactgca
     4741 taaaagcctc taatcactaa aggtgaagag gaacgcagtt ggatgctaag gcacaactgc
     4801 ataaaagcct ctaatcgcta aagatgaaga ggaacgcagt tggatgctac cgcacaactg
     4861 cataaatccc tctaatcgct aaagcgaaaa gtgggattaa aaaggagatg tgataatgtg
     4921 aagaaatcgt taattgcttt tattttgatt tttatgcttg tcctgagtgg ctgtggtatg
     4981 aaagataatc ataaacaagg tagcgatgat aatggctcgt ctaaatcgcc gtaccataga
     5041 attgtttcgt taatgcctag taatactgaa attttatatg aattaggatt aggtaaatac
     5101 atagttggtg tttcaacggt tgatgattat ccaaaagatg tgaaaaaggg taagaaacaa
     5161 tttgatgctt tgaatctaaa taaagaggaa cttttaaagg caaagccaga tctaattctt
     5221 gcgcatgagt cgcaaaaggc aactgctaat aaagtattgt catcattaga gaaacaaggc
     5281 atcaaagtag tatatgttaa agatgcacaa tcaattgatg aaacttacaa cacatttaag
     5341 caaattggga aattaacgca tcatgataag caggctgaac aacttgttga ggaaactaaa
     5401 gataatatcg ataaagtcat agattcaatt cctgctcatc ataaaaaatc aaaagtattt
     5461 attgaggttt catcaaagcc tgaaatatat acagcaggga agcatacatt tttcaatgat
     5521 atgttagaaa aattagaagc ccaaaatgtg tatagtgaca ttaatggttg gaaccctgta
     5581 acgaaggaaa gtattattaa aaagaaccca gatatattaa tttcgacgga agctaagaca
     5641 agatcagatt atatggatat catcaaaaaa agaggtggat tcaataaaat taatgctgtc
     5701 aagaatacac gtattgaagt tgtaaatggt gatgaagtat caagaccagg tccacgtatt
     5761 gatgaaggat taaaagaatt aagagatgca atttatagaa aataaaccat tctaattatg
     5821 ccccttattg ctacatgtaa aaaaatacat gtttgagata aggggttttt aaaatatatt
     5881 tagtgaatga tagcaacgcg agtatgtgat tgctataatg aatgtaatta tcgatgaaag
     5941 aaaagagaat gctatgacat ttaataaagt attattgagc tggatagtca tattgattat
     6001 aacaactagc atatatctat tttggcagtt gggcgatatc aatgatgtat ttaaccagtc
     6061 tattctaatc aatgttagat taccgagatt attagaagca ttgttgacag gtatgatatt
     6121 aactgttgca ggccttatat ttcaaacagt tttaaataat gcattggcag atagctttac
     6181 attaggattg gcaagcggcg ctacatttgg ttcaggatta gcattatttt taggtttaac
     6241 aacgttatgg attcctgtat tttcaataac atttagtttg ataacattaa taactgtatt
     6301 agtcattacg tcggtattga gccaaggcta tccagttaga atcttaatat taagtggttt
     6361 aatgattggt gcgttattca attcacttct atattttttg attttattaa aacctcgcaa
     6421 attaaataca attgccaatt atctgtttgg tggttttggt gatgcagaat actcaaatgt
     6481 atccataata gcaatcacat ttatcattgc actgtttggt atatttatca ttcttaatca
     6541 actaaagtta ttgcaattag gagaactaaa aagtcagtca ctaggcttaa atgttcaatt
     6601 gattacatat atcgcgttat gtatagcttc tatgataacg gcgataaatg tcgcatatgt
     6661 tggcatcatt ggattcattg gtatggtgat accacaactc attagaaaat ggcagtggaa
     6721 acaatcatta ggaagacaat tggctttgaa tattgtaatt ggaggacaaa taatggttat
     6781 ggcagatttt attggtagcc atatactgtc accagtacaa ataccggcaa gcattattat
     6841 tgcattaatt ggtataccag tgttatttta catgctaata tctcagtcga aacggttaca
     6901 ctagcacatg acatttgcta aaataaaaat aactataaac ataaagaggg cataagcgat
     6961 ggatttgaat caaattaaag cagttgtatt tgatttagaa ggtacgttgt tggacagagt
     7021 taaatctcga gagaaattta tcgaagagca atatgaacga tttcatgact acttaattca
     7081 tgttcaactg gcagatttta aaaaagcatt tattgagcta gatgacgatg aagataatga
     7141 taaacctgat ttatataaag aaatcattaa acgtttccat gtagataggt taacttggaa
     7201 agacttattt aatgattttg aaatgcattt ttatcgttat gtatttcctt attacgatac
     7261 tttgtataca ctagaaaagc tatcgcaaaa aggctttcaa attggtgtta tcgcaaatgg
     7321 taaatctaag attaaacaat ttcgattaca ttcacttggt ttgatgcatg ttattaatta
     7381 tttatcaaca tcagaaacag ttggttttcg taaaccacat cctaaaattt ttgaagatat
     7441 gattgatcaa ctaggggtat tacctgagca aattatgtat gttggcgatg atgcgttaaa
     7501 tgatgtagct ccagcacgag ctatgggcat ggttagtgta tggtataaac aagaagatgc
     7561 tgaaattgaa ccactcgaag aagaagttga ttttacaatt acaacagtgg aagaattatt
     7621 aaccatttta ccaataaaaa atgataataa aggagaaaat tatggatcta tttactagaa
     7681 aagatggaac atcgatacat tacagtacat taggtgaagg atatcctatc gtattgattc
     7741 atactgtact tgataattat tctgtgttta ataaattagc agcagaatta gcaaaatcat
     7801 ttcaagttgt gttaattgat ttacgtggac atggctattc tgataaacct cgtcacattg
     7861 aaataaaaga tttttctgat gacattgttg aattacttaa atatttatat attgaagaag
     7921 ttgcatttgt atgccatgaa atgggtggaa tcattggtgc ggatatttca gtacgttatc
     7981 ctgaatttac atcatcactt acgttggtaa atccaacatc tattgaaggt gaattaccgg
     8041 aagaacgttt atttagaaaa tatgcccata ttattcgaaa ctgggatcct gaaaaacaag
     8101 ataaattttt aaataagcgt aagtattatc gtccgagaaa aatgaatcga ttcctcaaac
     8161 atgtcgtaga tacaaatgaa atatcaacta aagaagaaat tcaagcagtt aaagaggtat
     8221 tcaaaaacgc tgatatttct caaacttata gaaatgtcgt agtaccgaca aaaattattg
     8281 caggagaatt cggtgaaaga acaacaagat tagaagctaa agaagtagct gatttaatcc
     8341 aaaatgcgga ctttgaagta tatcaagaat caagtgcatt cccatttgtt gaagagcaag
     8401 aaagattcgt cgaagataca gcttcattta tcaacaaaca tcacgatgaa aagcatgttt
     8461 aatttttagt gataagtgaa atttaaaagt agtaattatt atttgtaata taattgtaat
     8521 atgactgttg ttttagaaat gattgttgta taatggttgt gagcacaaga cgatataaat
     8581 aaaaggagag agtgcttatg aaaaagctac taacggcaag tataattgca tgttctgttg
     8641 taatgggagt aggcttagtg aacactagtg ccgaagcagc aagtggcaac tctattgata
     8701 ctgttaaaca attaattaag ggtgatcagt cattagaaaa tgtgaaaatt ggcgaatcta
     8761 ttaaagatgt tttaactaag tacaaaaatc cgatgtattc ttacaatgaa gatggaactg
     8821 aacattatta cgaattccat actaaaaaag gtatgttatt agtaactact gatggtaaga
     8881 aaaacaatgg taaagtaact catatttcaa tgatgtataa tgatgctaat ggtccaacat
     8941 atcaagctgt taaaaattat gttggcaaag cagtaacaca tacggaatat agcaaagttg
     9001 ctggtaattt tggatatatt gaaaaaggca aaacgactta tcaatttgcc tcagcaccaa
     9061 aagataaaaa cataaaatta tatcgtattg atttggaaaa ataataatta aattaatcgt
     9121 gcttaaccga caagttaaat cgcaatactt gtcggttatt
//
LOCUS       DAHHOJ010000102         9137 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.102, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000102 DAHHOJ010000000
VERSION     DAHHOJ010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9137)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9137)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9137
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.102"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(571..2571)
                     /locus_tag="NBH32_002139"
     CDS             complement(571..2571)
                     /locus_tag="NBH32_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515718.1"
                     /translation="MKLRLLWHIVRRQFITQRLVIIPFILAVSVLFMIEYTLVSIGLN
                     SYIKQKNDFLVPFIIIANFFMALLTFIFIFYANHFMMSQRRKEFSIFMTLGMTKKSMR
                     LIVVMETILQFMIISVVSIAGGYLLGAIFFLFIQKIMGSEVATLRYYPFDSVAMFITL
                     IIIAVLMGMLLIFNLFSINFQRPITYQHRSDSSVISRWLRYVLIVIGSAALYLGYFIA
                     LQQDTTFGAFFKIWIVIGFVIIGTYAFFVGISEIIISILQQVSKVYYHPRYFFVVVGM
                     RVRLKMNAVSLATITLLCTFLIVTLTMTLTTYRDMNHTIMKLITNDYDLSFNDNSKSQ
                     IERQQMIENIKRDIQGSVNAKDFKVYETTLFRASLEKNRAYYKLKQASDDIPIDFIGN
                     EGSIFSKQSVMITALTVQDYNRYHQNKLHLDNQSIGMITNVPIFKKQSKVGLNNEVFK
                     VKQLDAHALNLMMIQDSMVLIVKDNNQLQKVKRFYAHEQKKSTISINFNVSGKTKLTT
                     SQIQKLSKKYDASINSKSEMLMVWDRLTGGLIFVGGVVSIVLVIGIFLMMYYKQVSEA
                     YANQHNYDIMKKLGLDNGRIAKITRNQMTFLFAIPITVALIHTLISSNIVYTLLNMIG
                     INNYHIFLTSYVLAVIIISFLYMAMYKITSYIYAKVIHQERN"
     gene            complement(2568..3323)
                     /locus_tag="NBH32_002140"
     CDS             complement(2568..3323)
                     /locus_tag="NBH32_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515719.1"
                     /translation="MLLEVKHVKKVYGKGLNATTALNQMNLSVGAGEFVAIMGESGSG
                     KSTLLNLIASFDGLTEGDIIVDGAHLNNMKNESKALYRQQMVGFVFQDFNLLPTMTNK
                     ENIMMPLILAGAKRKDIEQRVHQLTVQLHLEGFLNKYPSEISGGQKQRIAIARALVTK
                     PTILLADEPTGALDSKTSKTLMMLFQEIHQLEQTILMVTHSNIDASYAERVIFIKDGR
                     LYHEIYRGEESQLAFQQRITDSLALVNGGSVNI"
     gene            complement(3431..4318)
                     /gene="nsaS"
                     /locus_tag="NBH32_002141"
     CDS             complement(3431..4318)
                     /gene="nsaS"
                     /locus_tag="NBH32_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system sensor histidine kinase NsaS"
                     /protein_id="HCD1515720.1"
                     /translation="MTFLKSITQEIAIVIVIFALFGLMFYLYHLPLEAYLLALGVILL
                     LLLIFIGIKYLSFVKTISQQQQIENLENALYQLKNEQIEYKNDVESYFLTWVHQMKTP
                     ITAAQLLLERDEPNVVNRVRQEVIQIDNYTSLALSYLKLLNETSDISVTKISINNIIR
                     PIIMKYSIQFIDQKTKIHYEPCHHEVLTDVRWTSLMIEQLINNALKYARGKDIWIEFD
                     EQSNQLYVKDNGIGISEADLPKIFDKGYSGYNGQRQSNSSGIGLFIVKQISTHTNHPV
                     SVVSKQNEGTTFTIQFPDE"
     gene            complement(4329..4994)
                     /gene="nsaR"
                     /locus_tag="NBH32_002142"
     CDS             complement(4329..4994)
                     /gene="nsaR"
                     /locus_tag="NBH32_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nisin susceptibility-associated two-component
                     system response regulator NsaR"
                     /protein_id="HCD1515721.1"
                     /translation="MKILIVEDDFVIAESLASELKKWNYGVIVVEQFDDILSIFNQHQ
                     PQLVLLDINLPTLNGFHWCQEIRKTSNVPIIFISSRIDNMDQIMAIQMGGDDFIEKPF
                     NLSLTIAKIQALLRRTYDLSVANDSMTVKGCTLILDEAKVVYQEQNIQLSLTELQILK
                     LLFQNEDKYVSRTALIEKCWESENFIDDNTLAVNMTRLRKKLNTIGVNDFIITKKNVG
                     YKV"
     gene            complement(5020..5220)
                     /locus_tag="NBH32_002143"
     CDS             complement(5020..5220)
                     /locus_tag="NBH32_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515722.1"
                     /translation="MILVMLSPLLIIFFIVLSILEERKRTKKKQLEKEKANTLNQNTN
                     DMESSNQELSLQQAKEQKDNKG"
     gene            5418..6842
                     /locus_tag="NBH32_002144"
     CDS             5418..6842
                     /locus_tag="NBH32_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCD1515723.1"
                     /translation="MLSVKKATKFAVANLIIVSTVFSSITPAIAQSDKSSKDESFAVG
                     NTKNPKNVIFLVGDGMGPSFNTAYRYYKNDPHAKEMTPTAFDSYLKGTNRTYSNDPKQ
                     NITDSAAGGTAFSSGHKTYNGAIGVDSNKQKVKTVLERAKEKGKSTGLVSTAELTDAT
                     PAAYAAHVTSRDDKNEIAKQFYKDKINGKHKVDVLLGGGAKYFGKSNGNLDKKFKKDG
                     YDLATNSKELSKSDKDKVLGLFADKNMPLAIDASKDEPSLADMQQSALSKLERNKKGF
                     FLMVEGASIDKSAHSNDITGVMSEMEGFEKAFDDAIQYAKKHKDTLVVATADHSTGGL
                     TIGKDKGYEWNPQPIKSMKHSGSYMTEKMAKGEDPEKVINEGYGFEFSQDDMKKVKKE
                     NKKLQKLLKKNKDEKSADVEKQTKALQHAIQKPVNDKSYTGWTSDGHTGEDVNTYAYG
                     PRSESFSGNIENTESAKIIFDIFK"
     gene            6912..7022
                     /locus_tag="NBH32_002145"
     CDS             6912..7022
                     /locus_tag="NBH32_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515724.1"
                     /translation="MLGAWLTRSWYFLNAKYKPPTNVKITANFFIIQTPF"
     gene            complement(7125..7580)
                     /locus_tag="NBH32_002146"
     CDS             complement(7125..7580)
                     /locus_tag="NBH32_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1515725.1"
                     /translation="MSTNKNDYEHMLFYFAYKTFITTADEIIEKYGMSRQHHRFLFFI
                     NKLPGITIKSLLEILEISKQGSHATLQKLKEQGLIIEKVLETDRRVKKLYSTDKGDQL
                     IAELNKAQDELLQNIYQQVGSDWYDVMEALAKGRLGFDFIKHLKDEKES"
     gene            complement(7826..8587)
                     /locus_tag="NBH32_002147"
     CDS             complement(7826..8587)
                     /locus_tag="NBH32_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HCD1515726.1"
                     /translation="MAYISLNYHSPTIGMHQNLTVILPEDQSFFNNDTTAKPLKTLML
                     LHGLSSDETTYMRYTSIERYANEHKLAVIMPNVDHSAYANMAYGHSYYDYILEVYDYV
                     HQIFPLSKKRDDNFIAGHSMGGYGTIKFALTQGDKFAKAVPLSAVFEAQNLMDLEWND
                     FSKEAIVGNLSSVKGTEHDPYYLLDKAVAEDKQIPKLLIMCGKQDFLYQDNLDFIDYL
                     SRKNVPYQFEDGPGDHDYAYWDQAIKRAITWMVND"
     gene            complement(8703..>9137)
                     /locus_tag="NBH32_002148"
     CDS             complement(8703..>9137)
                     /locus_tag="NBH32_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1515727.1"
                     /translation="SDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSDSD
                     SDSDSDSDSDSDSDSDSDSDSDSDSDSRVTPPNNEQKAPSNPKGEVNHSNKASKQHKT
                     DDLPETGDKSENTNATLFGAMMALLGSLLLFRKRKQDHKEKA"
BASE COUNT         3023 a         1549 c         1352 g         3213 t
ORIGIN      
        1 tgaaacaaat ttgactcatc tttttcaata tctaaatgac acgatgcatg atttttgaat
       61 tttgtaatta atttccttta aaattagact gaattatgac aattttcaaa cattttaatg
      121 tgtaaatcat tggcaaaata tacgaattgc atttaaatcg tcaatttaca tatatttttg
      181 cgtagtaatc aatcgttatc attgttattt atcgttacat tacttcgagt atcagtatgt
      241 atttcgggct tcgtttgata acgacatttc tttgtgacat cgcttcatca gtgcaacaat
      301 aaatacaatg atttcgtgat gttagttacc cattttatgt gttgcataaa atatgttgtt
      361 ataaaacatt ttaaatcatt ttatataaac aagctataca tttttgatat ttcaaaaata
      421 taacttgtca tacaagtaaa aaaaccgaca agacatttta tcttatcggt tgaaatttgt
      481 tattgttatt tgtaatgttt ttaggtttct ttttaatata atatatttca gtgaaaatac
      541 atgattgatt atgatttcac tgaaacatgg ttaattacgt tcttgatgaa taactttagc
      601 ataaatatag gaagttattt tgtacatcgc catatataga aacgaaatta taatgacagc
      661 tagtacgtaa cttgttaaaa atatatgata gttattaata cctatcatat ttagtaacgt
      721 atatacaatg ttactagaaa ttaatgtatg aatcagtgct actgttattg gtattgcgaa
      781 caagaaagtc atttgatttc gtgttatctt tgctattctt ccattatcta aaccaagttt
      841 tttcataatg tcataattgt gctgatttgc ataggcttct gaaacttgtt tatagtacat
      901 cattagaaat attccaatga caagtacaat tgatactacg ccacctacaa atattagtcc
      961 acctgtcaga cgatcccaaa ccattagcat ttctgattta gaatttattg acgcatcgta
     1021 ttttttcgat agtttttgta tttgtgatgt tgttaatttc gtctttccag atacattaaa
     1081 gtttatcgat attgtcgact tcttttgttc atgtgcataa aatcttttta ctttttgtaa
     1141 ttgattgtta tctttaacaa ttaaaaccat actatcttga atcatcatca aatttaacgc
     1201 atgtgcgtca agttgtttca ctttaaagac ttcattatta agaccgacct tagactgctt
     1261 tttaaaaata ggtacattcg taatcatacc tatactttga ttgtctaagt gtaatttgtt
     1321 ttggtgatat cgattataat cttgcacagt aagtgctgtg atcattactg attgcttaga
     1381 aaatatcgag ccttcattac caatgaagtc tattggaata tcatcactag cctgttttaa
     1441 cttgtaataa gcacgattct tttctaaaga agctctaaat aatgtcgttt catatacttt
     1501 gaagtctttt gcattaaccg aaccctgtat atcacgttta atattctcaa tcatttgttg
     1561 acgttctatt tgcgacttag aattgtcgtt aaatgacaaa tcataatcat tggtaatcaa
     1621 tttcataatg gtatgattca tatcgcgata ggttgttaat gtcattgtga gcgttacaat
     1681 caaaaatgta cacagcaaag tgatcgttgc aagactgact gcattcattt taagacgtac
     1741 acgcatccca actaccacaa aaaaataccg tggatgatag taaacttttg atacctgctg
     1801 caatatacta ataattattt cacttatacc tacaaaaaat gcataagtac cgataataac
     1861 gaatcctatg acaatccata ttttaaaaaa ggcaccaaac gtcgtatctt gttgtaatgc
     1921 aataaagtag cctaaatata gtgctgcgct tcctataaca attaaaacgt aacgcaacca
     1981 tcgtgatatg acactggaat cagaacgatg ttgataagtt atcggtcgtt gaaaatttat
     2041 actgaacaag ttgaatataa gtagcatgcc cattaataca gcaatgataa tcaaagtaat
     2101 aaacatcgct acagagtcaa atggataata ccttaacgtc gcaacctcac tgcccattat
     2161 tttctgtata aacaagaaaa atatcgcacc aagtaagtat ccgccggcaa tactaacgac
     2221 tgaaattatc ataaattgta agatagtttc catcactaca attaaacgca tacttttctt
     2281 ggtcatgccc aatgtcataa aaatgctaaa ctcttttcgt ctttgtgaca tcataaagtg
     2341 atttgcatag aaaataaaaa taaaagttaa aagcgccata aaaaaattag ctatgataat
     2401 aaatggtact aggaagtcat tcttctgttt tatgtagcta tttaacccaa ttgacacaag
     2461 cgtatattca atcatgaata gtacacttac cgctaaaatg aatggaatga ttacaagtcg
     2521 ctgcgtaata aattgacgtc gcactatgtg ccataacaat cttaacttca tatattgaca
     2581 cttcctccat tcacaagtgc taagctatct gttattcgtt gttgaaaagc taattgactt
     2641 tcttcaccac gatatatttc atgatataga cgcccatctt taataaaaat gactcgctct
     2701 gcataagacg catcgatatt tgaatgagtt accattaaaa ttgtctgttc caattgatga
     2761 atctcttgaa ataacatcat caatgtcttt gatgtttttg aatcaagtgc acctgtaggt
     2821 tcatcggcta gtaaaatcgt cggcttagta actaatgcac gtgcaatggc aatgcgttgc
     2881 ttctgacccc ctgagatttc agaaggatac ttgtttaaga atccctctaa atgtaattgt
     2941 actgtcaact gatgtaccct ttgttctata tcttttcgtt tagcaccagc taaaattaat
     3001 ggcatcatta tattttcttt attcgtcatt gttggtaaaa gattaaaatc ttgaaaaaca
     3061 aaacctacca tttgttgacg atacaatgct ttactttcat ttttcatatt atttaaatgt
     3121 gcgccatcca caataatgtc accttcagtt agtccatcaa aagaagcaat taaatttagt
     3181 agtgtagact tccctgaccc agactcaccc ataattgcaa caaattcacc agctccaact
     3241 gataaattca tttgattaag tgctgtcgta gcattcaaac ctttaccata aacctttttt
     3301 acatgtttca cttctagcaa catggcatgc acctcatttc tttgttagtt ctatattaaa
     3361 acaaaaaaat actaataaca tttaaattag tgaaaaaatg ttactagtat gcttacaata
     3421 ttgaaagttt ttattcatct ggaaattgaa tcgtaaatgt tgtaccctca ttttgtttag
     3481 atacgactga aacaggatgg tttgtgtgag ttgaaatttg ttttacgata aataagccaa
     3541 tcccacttga gttactttga cgctggccat tataacctga atagccctta tcaaatattt
     3601 taggcaagtc cgcctcacta ataccgatac cattatcttt tacatataat tgattggatt
     3661 gctcatcaaa ttcaatccat atatctttac ctctcgcata cttaagtgca ttatttatta
     3721 attgttctat cattaaagag gtccatctaa cgtcagttaa tacttcgtga tgacaaggtt
     3781 cataatggat ttttgttttt tgatcaataa actgtattga atatttcata ataattgggc
     3841 gaatgatatt gttaatcgaa attttagtga cagaaatatc agaagtttca tttaataact
     3901 ttaaataact aagtgctaaa cttgtatagt tatcaatttg aataacctct tgacgaacac
     3961 gattcacaac attaggctca tctctttcaa gtaacagttg tgctgcagta atgggtgttt
     4021 tcatttgatg tacccatgtt aaaaagtagc tctctacatc atttttatat tcaatttgtt
     4081 catttttaag ctgatacaac gcattttcta agttttcaat ttgttgttgt tggcttatag
     4141 tttttacaaa acttaaatat ttaataccta tgaatatgag taataataat aaaataacgc
     4201 caagtgctag taaatatgct tctaatggca aatgatacag gtaaaacatt aagccaaata
     4261 aagcaaaaat aactatgact attgctattt cctgagtaat acttttaaga aaggtcattg
     4321 cattcaccct atactttata tccgacattt ttctttgtaa taataaaatc attaacgcca
     4381 atagtattta attttttacg caggcgcgtc atgttaacag ctaatgtatt gtcatctatg
     4441 aagttttctg attcccaaca tttttcaatt aaagccgttc tacttacata tttatcttca
     4501 ttttgaaata ataactttaa tatttgtaat tcagtcaaag atagctgtat gttttgttct
     4561 tgatacacga cttttgcttc atctaatatt aatgtacaac ctttaaccgt catcgaatca
     4621 ttagctactg acaagtcata agttcgtctc aataatgctt gaattttggc aatcgttaat
     4681 gacaagttaa atggtttttc gataaaatca tctcccccca tttgtattgc cataatttgg
     4741 tccatattat caatacggga actaataaat ataattggca cattagatgt ttttcggatt
     4801 tcttgacacc aatgaaaacc atttaacgtt ggcaaattaa tatctagcaa tacaagctga
     4861 ggttgatgtt ggttaaagat agacagtata tcatcaaatt gttcaacgac aataacaccg
     4921 taattccatt ttttaagttc agatgctaaa ctttctgcta taacaaaatc atcttcaaca
     4981 attaatattt tcatctataa tcttcttcct tcaattgaat tatcctttgt tatctttttg
     5041 ttctttagcc tgttgcaatg acagctcttg atttgaactt tccatgtcat ttgtattttg
     5101 atttagtgta tttgcttttt ctttctcgag ttgctttttc ttcgtacgtt tacgctcttc
     5161 taaaatagac aacactataa agaatatgat taataatgga gataacatta ctagtatcat
     5221 ttttccacct caaattatat ttaattctat attttcacac gcttctattc taacgctttc
     5281 tataacaaat tgtcatatat attcattttt tgttattcta aatcactctg tttacatatt
     5341 tttaactaaa actacataat tgctcaaaat tcctctttta cactgaagac atagtcatat
     5401 actcaggagg atgtcttatg ttatcagtta aaaaagccac aaaatttgca gttgctaacc
     5461 ttattatcgt gtcaacagtt ttcagttcta tcacacctgc aattgcgcaa tccgataaaa
     5521 gttctaaaga tgaaagtttc gcggttggta atactaaaaa tcctaaaaat gttatctttt
     5581 tagttggcga tggtatgggt ccatcattta acacagctta tcgttattat aaaaatgatc
     5641 cacatgccaa agaaatgacc cctactgctt ttgattcata tttaaaaggc acaaatcgta
     5701 catattcaaa tgatccaaaa cagaatatta ctgattctgc agctggcgga acagccttta
     5761 gttctggcca taagacatat aatggtgcca tcggtgttga cagtaacaag cagaaagtaa
     5821 agacggtgct tgaacgtgca aaagaaaaag ggaagtcgac tggtcttgtt tcaacagctg
     5881 aattaacaga tgcaacacca gcagcatatg ctgcacacgt gacttctaga gatgataaaa
     5941 acgaaatcgc taaacaattt tataaagata aaatcaacgg aaagcataaa gttgatgttt
     6001 tattaggcgg tggcgctaaa tatttcggta aaagtaatgg caatttagat aaaaaattca
     6061 aaaaagatgg ttacgacctt gccacgaaca gtaaggagtt atcaaaatct gataaagata
     6121 aagttcttgg tttgtttgca gataaaaata tgccacttgc aatagatgct tctaaagacg
     6181 aaccatcatt agctgatatg caacaaagtg cacttagtaa gcttgagcgt aataaaaaag
     6241 gtttcttttt aatggtcgaa ggtgcttcta tagataaatc agcgcattct aacgatatta
     6301 caggcgttat gtctgaaatg gaaggttttg aaaaagcatt cgatgacgcc attcaatatg
     6361 ctaagaaaca taaagataca cttgtcgttg caacagcaga tcactctact ggtggtctaa
     6421 caattggtaa agataaagga tacgaatgga atcctcaacc gattaaatcg atgaaacact
     6481 ctggatcata tatgacagaa aaaatggcta aaggtgaaga ccctgaaaaa gttattaatg
     6541 aaggttatgg ctttgaattt tcacaagatg atatgaaaaa ggttaaaaaa gagaataaaa
     6601 agttgcaaaa attacttaaa aaaaataaag atgaaaaatc tgcagatgta gaaaaacaaa
     6661 caaaagcctt acaacacgct attcagaagc cagtaaatga taaatcttat acagggtgga
     6721 cttccgatgg acatacaggc gaggatgtta atacctacgc ttatggacct agatcagaat
     6781 catttagtgg taatatcgaa aatacagaaa gcgcaaaaat aatatttgat atattcaagt
     6841 aaagcataaa aaaattcccg agcactataa caaggtgttc gggaattttt tatggtttaa
     6901 tttagtactc aatgttaggt gcttggttaa cacgttcttg gtatttttta aatgcgaagt
     6961 ataaaccgcc aactaatgtt aaaataactg ctaatttttt catcattcaa actcctttct
     7021 aagatgtgtt gaaactgagt tcaattaaat tatatgtttt tattatacac tttttgacat
     7081 attttttaaa tttaagaatg cgaagatttt taacatttct gatgctagct ttctttttca
     7141 tctttcaaat gcttaataaa atcaaagcca agtcgccctt tagccaatgc ttccatcaca
     7201 tcataccaat ccgaaccgac ttgttgatat atattttgca ataattcatc ttgcgccttg
     7261 ttcaattcag caatgagttg atcgccttta tccgtcgaat ataatttttt gacacgtcga
     7321 tcagtctcta aaactttttc aataatgaga ccttgctctt ttaatttttg aagtgttgca
     7381 tgtgatcctt gtttagaaat ttctaatatt tctagtaatg atttaatagt aataccaggt
     7441 aatttattga taaaaaacaa aaaacgatga tgctgacgac tcataccata cttctctata
     7501 atttcatcag cggtagtaat aaacgtttta tatgcaaaat aaaacaacat atgctcataa
     7561 tcgtttttat tggtcgacat gatgatgact cctttcaaag cgttaacctc attataataa
     7621 aatttataat gtaagtcata caaatgatga taaaactata agctaaatct aagtttcacg
     7681 agaaacattt ttgtgtatca gcaaattgcg ccaatcatat taatacaaat ttaatttaaa
     7741 cacttagaaa tgttagtcgt gcgtaaatag tttgtatctc tcactctgtg tattttgatg
     7801 caatcacata ttttccagga aataattaat cattcaccat ccatgttata gcacgcttaa
     7861 tcgcttggtc ccaatatgca taatcatgat ctcctggtcc atcttcaaat tgataaggaa
     7921 catttttgcg tgataaataa tcgataaaat ctaagttgtc ttgatataaa aagtcttgtt
     7981 taccacacat aatgagcaat tttggaattt gtttatcttc agctacagct ttgtctagca
     8041 aataatacgg atcatgttca gttcctttaa cacttgaaag attgccaact atggcctctt
     8101 ttgaaaaatc attccactct agatccatta aattttgcgc ttcaaacaca gcagataatg
     8161 gtacagcttt ggcaaattta tcgccttgtg ttaatgcaaa tttaattgtg ccatatcctc
     8221 ccatagagtg acctgctata aaattgtcat cacgcttttt ggaaagtgga aatatttgat
     8281 gaacataatc atatacttcc aaaatataat cataatagct atgaccatat gccatgttag
     8341 catatgcgct atgatccaca ttgggcataa tcacagctaa tttgtgttca ttcgcatacc
     8401 tttctatgct tgtatatctc atatatgtcg tttcatcact tgataatcca tgtaacaaca
     8461 ttaaagtttt taatggttta gcagttgtat cgttattaaa gaagctttga tcttccggta
     8521 aaatgactgt caaattttga tgcataccaa ttgttggtga atgatagttt aatgaaatat
     8581 aagccatacg tcatgacccc tttctaattc ttctttatca acattttacg cttaaacaat
     8641 tcactttaaa atcattttca acaaaaaaac cgaatacaaa tgtattcggc ctaaaaaagt
     8701 atttacgctt tttctttatg atcttgctta cgttttctaa acaatagtaa tgatcctaat
     8761 aatgccatca ttgcaccaaa taaagttgca tttgtgtttt cgctcttatc tcctgtttct
     8821 ggtaaatcat cagttttgtg ttgttttgat gccttattag aatggtttac ttcaccttta
     8881 ggatttgatg gtgctttctg ttcattattt ggtggtgtaa ctcttgaatc ggagtcacta
     8941 tctgagtctg agtcactgtc tgagtccgag tcactgtctg agtctgagtc tgaatcgcta
     9001 tctgagtccg agtcgctgtc tgagtctgaa tcgctatctg agtccgaatc gctgtctgag
     9061 tctgaatcgc tatctgagtc cgaatcgctg tctgagtctg agtcgctatc tgaatctgaa
     9121 tcactatctg agtctga
//
LOCUS       DAHHOJ010000103         9001 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.103, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000103 DAHHOJ010000000
VERSION     DAHHOJ010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 9001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9001
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.103"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(170..658)
                     /locus_tag="NBH32_002149"
     CDS             complement(170..658)
                     /locus_tag="NBH32_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000154459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515728.1"
                     /translation="MTINIIEYNRSYKEELIEFILSIQKNEFNIKIDRDDQPDLENIE
                     HNYLNSGGQFWLAINNHQNIVGTIGLIRLDNNMSALKKMFVDEGYRNLKIGKKLLDKV
                     IMTCKEQNIDGIYLGTIDKFISAQYFYSNNGFREIKRGDLPSSFPKLDVDNRFYYRNL
                     KG"
     gene            complement(934..1635)
                     /gene="isaA"
                     /locus_tag="NBH32_002150"
     CDS             complement(934..1635)
                     /gene="isaA"
                     /locus_tag="NBH32_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase IsaA"
                     /protein_id="HCD1515729.1"
                     /translation="MKKTIMASSLAVALGVTGYAASTGHEAHAAEVNVDQAHLVDLAH
                     NHQDQLNAAPIKDGAYDIHFVKDGFQYNFTSNGTTWSWSYEAANGQTAGFSNVAGADY
                     TTSYNQGSNVQSVSYNAQSSNSNVEAVSAPTYHNYSTSTTSSSVRLSNGNTAGATGSS
                     AAQIMAQRTGVSASTWAAIIARESNGQVNAYNPSGASGLFQTMPGWGPTNTVDQQINA
                     AVKAYKAQGLGAWGF"
     gene            complement(2243..3298)
                     /locus_tag="NBH32_002151"
     CDS             complement(2243..3298)
                     /locus_tag="NBH32_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD1515730.1"
                     /translation="MTSIKTITPKDFIFRVLSGVAIGIVAGLVPNAILGEIFKYFMDY
                     HPIFKTLLGVVVAIQFTVPALIGALVAMKFDLSPLAIAVVASAAYVGSGAAQFKNGAW
                     MITGIGDLINTMITAAIAVLFILLIQHRVGSMALIVFPTVVGGISGAIGVLILPYTKM
                     ITTAIGNMVNGFTELQPIVMSILISMVFSLIIISPLSTVAIAFAIGITGLAAGSASIG
                     ISATEAVLIIGTSKVNRLGVPLSVFFGGVKMMIPNMVKYPILMLPILTTAIVSGLVSA
                     LVGIHGTKESAGFGFIGMVGPINAFKFMEVDSAWLSVLLIVVAFFVVPFVTAWLADII
                     YRKVFRLYTNDIFKFMG"
     gene            complement(3425..3553)
                     /locus_tag="NBH32_002152"
     CDS             complement(3425..3553)
                     /locus_tag="NBH32_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515731.1"
                     /translation="MDFKNEDKIKVYCNNMFEKLPISLSKNSEFCDILFFIVLSYR"
     gene            3708..4277
                     /locus_tag="NBH32_002153"
     CDS             3708..4277
                     /locus_tag="NBH32_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1515732.1"
                     /translation="MMESKSIDPRIVRTKQLLVDAFLKISREKKLSQITVKDITDIAT
                     LNRATFYAHFTDKEDLLDYTLSVTILKDLNDNLSISNVINEKVLRNIFISIASYIKDA
                     AKSCELNSEAFCNKAHQRINNELEDIFAIMLENSYPEHQRDIIVNSASFLAAGISGLA
                     LHWFNTSQETADVFIDRNLPFLIHHIAHF"
     gene            4475..4975
                     /locus_tag="NBH32_002154"
     CDS             4475..4975
                     /locus_tag="NBH32_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515733.1"
                     /translation="MTSIFVFAYLNNTSDNMLFRQQMIYACLLANVRHSSIVCLNNTC
                     RIVDIRCLNTKIGGIDVSETDESQGFERTHNILNINQSSLGVVTYITNKLKSTLKQHI
                     IIACGKKRIDYRLSYNFYTTYYDNVEIKKIDCEYTYAMPIAILIRHMMSFDNDYFFAQ
                     LRHPVL"
     gene            5195..5425
                     /locus_tag="NBH32_002155"
     CDS             5195..5425
                     /locus_tag="NBH32_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD1515734.1"
                     /translation="MKILDRINELANKEKVQPLTVAEKQEQHALRQDYLSMIRGQVLT
                     TFSTIKVVDPIGQDVTPDKVYDLRQQYGYIQN"
     gene            complement(5829..6206)
                     /locus_tag="NBH32_002156"
     CDS             complement(5829..6206)
                     /locus_tag="NBH32_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCD1515735.1"
                     /translation="MIQSMWFNLHVQDLEKSAQFYKALGFKINRNPQMLDKMVGIQIG
                     QTTVILIENKHFQNVSQQSLNTEPNEVMISLGVNTNEEVDQLVNKVKEAGGTVVQEPT
                     VSQGFYGAMFKDLDGHHFNFLVC"
     gene            complement(6223..7044)
                     /locus_tag="NBH32_002157"
     CDS             complement(6223..7044)
                     /locus_tag="NBH32_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA/HSCARG family protein"
                     /protein_id="HCD1515736.1"
                     /translation="MKDILVIGATGKQGNAVVKQLLEDGWYVSALTRNKNNRKLSDIG
                     HPHLSIVEGDLSDSDSLQSAMKGKYGLYSIQPIVKDDVSEELRQGMKIIEIAEQENIQ
                     HIVYSTAGGVNRNRTGPHFEVLAKIENRLMESNINATVIKPSFFMDNFLRIAKVEDER
                     ITLPEFINPNIKFTMISSIDIAKIASYIFAHPQSFTHQSIEIGSDEVTLSEAATIFSE
                     VTGKSTVIEGEFVSSVAEKQWLEEKGYEVDFELMAEINPTRLSLSDWLKVQDYNK"
     gene            complement(7063..7362)
                     /locus_tag="NBH32_002158"
     CDS             complement(7063..7362)
                     /locus_tag="NBH32_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2316 family protein"
                     /protein_id="HCD1515737.1"
                     /translation="MSLNKEQRRITAEELQAHFEASTLSVQMIAEKLNVTTEDVEKAL
                     AMKAPLGIFSHQLQRFIHLVWDVRNVINDNIKENGQTPEPYTYLKGEKEDYWFLR"
     gene            7493..8050
                     /locus_tag="NBH32_002159"
     CDS             7493..8050
                     /locus_tag="NBH32_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1515738.1"
                     /translation="MRKDAKENRQRIEEIAHKLFDEEGVENISMNRIAKELGIGMGTL
                     YRHFKDKSDLCYYVIQRDLDIFITHFKQIKDDYHSDYEVMQVSLDYLLQFKIDNKALL
                     QCIEAGNNKLRFYQSAFYQELFNFYYDLFKSDDDIYKKFKTDMLLQALSTSVFAFQIE
                     HRHISIEAYRNYLLNIYLDEVERND"
     gene            8043..8747
                     /locus_tag="NBH32_002160"
     CDS             8043..8747
                     /locus_tag="NBH32_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HCD1515739.1"
                     /translation="MTKIVLITGGNKGLGYASAEALKALGYKVYIGSRNDVRGQQASQ
                     KLGVHYVQLDVTSDYSVKNAYNMIAEKEGRLDILINNAGISGQFSAPSKLTPRDVEEV
                     YQTNVFGIVRMMNTFVPLLEKSEQPVVVNVSSGLGSFGMVTNPETAESKVNSLAYCSS
                     KSAVTMLTLQYAKGLPNMQINAADPGATNTDLVGDFSNNSKHVSEGIKPIIQLATIDA
                     DGPTGTFINGDGEMPW"
     gene            8845..>9001
                     /locus_tag="NBH32_002161"
     CDS             8845..>9001
                     /locus_tag="NBH32_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HCD1515740.1"
                     /translation="MKSITFEEHYVIEDIQKETMNAISADPKGVPMKVMLEGLEKKTG
                     FTNADELS"
BASE COUNT         3036 a         1604 c         1373 g         2988 t
ORIGIN      
        1 acttctataa tgaataaatt accatgtatt gaaaataaag tgaaatgcat aatatcctct
       61 aatttaaatc aaacgatata tcacgtcagc cttttatatt taaacgctat atgccacttt
      121 taaaatgaat attactaagg tcgccatatc aattattcat tgattcgaat taacctttta
      181 aatttctgta ataaaaccta ttatctacat ctaatttagg aaaactactt ggtaaatctc
      241 ctcgcttaat ttcacggaag ccattattag aataaaaata ctgtgcacta ataaatttat
      301 caattgttcc taaataaata ccatcgatat tttgttcttt acatgtcatt ataactttat
      361 ctaaaagttt tttacctatt tttaaattcc tataaccttc atcaacaaac atttttttaa
      421 gtgcagacat attattatct agtctaatca aacctatagt accaacaata ttttgatgat
      481 tgtttattgc aagccaaaat tgccctccac tattcaaata gttatgttcg atgttctcca
      541 aatcaggttg atcatctcta tcaattttta tattaaattc atttttttga atcgataaaa
      601 taaactcgat tagctcttcc ttataagacc tattatattc aattatgttt atagtcattt
      661 ttatctccag tttcatttaa tttaatcata aaatgtgcgt ttagtttgta tctagtgtac
      721 tcagtacagc ctcaaatgaa gtttcattcc acttggcact taataaagac aagtatttta
      781 gcagtaatac aataaagtcc aataaatttc cctaacttca atatccactt tttaagaaat
      841 gtatttttga ttaataaaaa aactctcccc aatttctatg ggaagagcta tatatttaat
      901 gtttaaacat tacttttatt tattatgaag gaattagaat ccccaagcac ctaaaccttg
      961 tgctttgtat gctttaacag ctgcgttgat ttgttggtca acagtgtttg ttggacccca
     1021 acctggcata gtttggaata aacctgaagc acctgatggg ttgtaagcat ttacttgacc
     1081 atttgattca cgagcgatga ttgcagccca tgtagaagct gaaacaccag tacgttgagc
     1141 catgatttga gctgctgatg aaccagtagc acctgcagta ttaccattgc ttaatctcac
     1201 tgaacttgaa gtagttgaag tgctgtagtt atggtaagtt ggagctgaaa cagcttcaac
     1261 gtttgagtta cttgattgtg cattgtagct tactgattgt acatttgaac cttggttgta
     1321 tgaagtagtg tagtctgcac ctgcaacgtt tgagaaacca gcagtttgac cattagctgc
     1381 ttcatagctc catgaccatg tagtaccatt tgaagtgaag ttatattgga aaccatcttt
     1441 tacaaagtgg atgtcatatg caccatcttt gattggagct gcatttaatt gatcttggtg
     1501 attatgcgct aagtcaacta agtgtgcttg atcaacgttt acttcagcag cgtgtgcttc
     1561 atgtcctgtg cttgctgcgt aacctgttac acctaatgcc actgctaatg atgatgccat
     1621 aattgtcttt ttcatagtaa aaaatcctcc agtaataatt gtaagtttat gtttttagta
     1681 attatatttt gaatttgaat gtcgtagtgc aagtttaaat tgtcttttat ttctttcaac
     1741 ggtactcact atatcataaa aaagtagcaa gtaaattaca ctttctttac aaaacattac
     1801 aatatcaagt gttatttgta atgttgaaat atggttgttt tatactgtaa tgtgaaatat
     1861 gtttcttttt gaatctaatc aatccttgaa ataagcataa atgcataagt tttttactat
     1921 atttagctca actattacag ctttcgtaat attacagatt gtatttttgt tacatgcttg
     1981 taacattacc gtcatcaaat acaacttaat ttgatcagtt aaacacaatt aaaaagcaat
     2041 caatacatag gttccatcac atgtattgat tgctattaat attaaatatt caaagtgact
     2101 atgcacatat cttttgtgga ttgacttatc ttttattagc gcaaattatc tgctgttcaa
     2161 ttatattgtt caacttcatt ctcgcaattc acaataacat taaataattt tcggtctcat
     2221 attttcaaaa aacatactgt ccttatccca tgaatttaaa aatatcatta gtatataaac
     2281 gaaacacttt acgataaatg atatctgcaa gccaagctgt tacaaatggt acaacaaaga
     2341 acgctactac aattagtaag acactcaacc aagcagaatc aacctccata aatttaaatg
     2401 cattaatcgg tcctaccatc cctataaaac caaatccagc tgattctttc gttccatgaa
     2461 tacctactaa tgctgatacc aaacctgata caatggctgt tgtcaatatt ggtaacataa
     2521 gaattggata tttcaccata ttaggtatca tcattttaac gcctccaaag aagacggata
     2581 acggcacccc taaacgattc actttacttg taccaattat caatactgct tcagtcgcgg
     2641 agataccaat tgacgctgat ccagctgcta aacctgtaat acctatcgca aaggcaatgg
     2701 ccacagttga tagtggagaa ataataataa gactaaatac cattgaaatc aaaatactca
     2761 tgacaatcgg ttgtaattct gtaaaaccat taaccatatt accgatggct gttgtaatca
     2821 ttttcgtata cggcaatatt aaaacaccaa ttgcacctga aataccgcca acaactgttg
     2881 ggaatacaat caatgccata ctacctacgc gatgttgaat aagtaaaatg aacaacactg
     2941 caatcgctgc tgtaatcatt gtattaatta aatcaccaat acccgtaatc atccaagcac
     3001 catttttaaa ctgcgctgca ccgcttccta catatgctgc actcgccaca acagcaattg
     3061 ctaatggtga taggtcaaat ttcatggcaa ccaatgcacc aatcaaagca ggtactgtaa
     3121 attgaattgc aacgacaacg cctaataacg ttttaaaaat cggatgataa tccataaagt
     3181 atttaaaaat ttctccaagt atcgcattag gaactaaacc cgcaacaata cctatggcga
     3241 cacctgataa aactctaaat ataaaatctt tgggtgtaat tgttttaatt gatgtcataa
     3301 tatcatcctt ccatttatgt atatacatct gtatgcaaat aataaagagc cttaagttat
     3361 aagctgccac tagcttaaat tctaagatgt gcatgccgat gttgttatat ttaggctaac
     3421 agtatcatct ataactcaag actatgaaaa atagtatatc acaaaattct gaatttttag
     3481 ataaagaaat tggcaatttt tcaaacatat tgttacaata cacttttatt ttatcttcat
     3541 ttttaaaatc cattaataca atagaagaaa gacattcaaa tgcttaccaa aaaggtacat
     3601 tatttgttag gagcgtatca gcacttacat atcatcaaca caattgacaa tataatagaa
     3661 gatactgata ataagtgtta aaacaacaga tgttaggtag tgaacaaatg atggaaagta
     3721 aatccataga tccaagaatc gttagaacca aacaattgct tgtcgatgct tttcttaaaa
     3781 tttctagaga aaagaaatta tctcaaatta cagttaaaga tatcactgat attgctacgc
     3841 taaatcgtgc aacattttac gctcatttca ctgataaaga agacctccta gactacacat
     3901 tgtctgtaac cattttaaaa gacttgaatg ataacttgag catttctaat gtcattaatg
     3961 aaaaggtact gcgtaatatt ttcatttcaa ttgcgagtta tatcaaagat gctgcaaagt
     4021 cttgcgaatt aaatagtgaa gcattttgca acaaagcaca tcaacgtatt aataatgaat
     4081 tagaagatat ttttgcgatt atgttagaaa acagctaccc agagcatcaa cgcgatatca
     4141 ttgtaaatag tgcgagtttt ttagcagctg gtatctcagg cttagcatta cattggttta
     4201 acacgagtca agagacagcc gatgtgttta tcgatcgcaa cctaccattt ttaattcatc
     4261 atatagcaca tttttaataa aacttagtat ttagtcatgc atcttgaaat cactatgtga
     4321 cttaggttca tacttgtaca cacaataaaa tttaacgtat tacgattaat ttgcagtgtc
     4381 taggacataa atcaacgtcc tatactctac aaagccatat tggcagtcgt taactgaatg
     4441 aaaataagct tgtcattaaa acatatagat tttagtgaca agcatttttg tttttgcgta
     4501 cttaaacaac acttcagaca atatgttgtt taggcaacaa atgatatatg cgtgtttatt
     4561 ggcaaacgta cgacatagta gtatagtatg tctaaacaac acatgtcgta tagttgatat
     4621 acgttgttta aatactaaga taggagggat tgacgtgagc gagacagatg aatctcaggg
     4681 gtttgaacgc acgcataata tattaaatat taatcagagt agtctgggtg tagtgacata
     4741 cattacaaat aaattaaagt cgacgttgaa gcaacacata ataattgctt gtggtaaaaa
     4801 gcgaatcgac tatcgactgt cgtataactt ttacactacg tattatgata atgtagaaat
     4861 caagaaaatc gactgtgaat atacctatgc tatgcccatt gcaattttaa taagacacat
     4921 gatgtcattc gacaatgatt atttctttgc tcagctacgt catcctgtct tataaaacaa
     4981 cattgcagac atgtatatca aacgacactt caataacatc agtttgccat cgtactacta
     5041 ttaaaatcgt gtctcaaacc ccttatttta attccaaaaa tctgctggtc aaaagaccga
     5101 gaaactaaaa acattactga atgtgttgat aaattacctt atagaaataa tctcaaaata
     5161 tatcaacact tgattctaag gaggatatga caatatgaaa attttagata gaattaatga
     5221 acttgcaaat aaagaaaaag tacaaccact tactgtagct gaaaaacaag aacaacatgc
     5281 attgcgtcaa gactacttaa gcatgatccg aggacaagta ttaacaacat tttccacaat
     5341 aaaagtggtt gatccaatcg gtcaagatgt cacaccagat aaagtttatg atcttcgcca
     5401 acaatacggt tatattcaaa attaatattt gctcacgagg tattgcactt aaggtgccaa
     5461 ctgacctcat aaacaaagcc catactgatt gaagacacta atgtgtccag gctgggacat
     5521 aaatcaatgt tctatgctct acgaagttat attggcagta gttgactgaa cgaaaatgcg
     5581 cttgtaacaa gcttttttca attctagtca ggggccccaa cacagagaat ttcgaaaaga
     5641 aattctacag gcaatgcaag ttggggatgg gccccaacaa agagaaattg gattcccaat
     5701 ttctacagac aatgcaagtt ggggtgggac gacgaaataa attttgcgaa aatatcattt
     5761 ctgtcccact cccacattaa acttcatctc tgtatgggct ttttatttat tcttttgaga
     5821 atttcatttt agcagaccaa aaaattaaaa tggtgtccat caagatcttt gaacattgca
     5881 ccataaaacc cttggcttac tgttggttct tgaacgactg tgccacctgc ttctttcact
     5941 ttattcacca actggtcaac ttcttcattt gtgttcacac ctagagaaat catcacttca
     6001 tttggttcag tattaaggct ttgctgactt acattttgaa aatgcttgtt ttctattaaa
     6061 attacggttg tttgacctat ttgaatgccc accattttat ctaacatttg tgggtttcta
     6121 tttattttaa atcctaacgc tttataaaac tgtgcgctct tttctaaatc ttgaacatgc
     6181 aaattaaacc acattgattg aatcatgatt gcaccccatt cattacttat tatagtcttg
     6241 gactttaagc caatcactta atgataatct tgttggattt atttcagcca ttaattcaaa
     6301 gtctacttca taaccttttt cttccaacca ttgcttttct gcaacactac taacaaattc
     6361 tccttcaata acagtcgatt tacctgtcac ttcactaaaa attgttgctg cttcacttaa
     6421 tgtaacttca tcggaaccaa tctctattga ttgatgcgta aagctttgtg gatgtgcaaa
     6481 aatatacgat gcaattttag ctatatcaat agaagaaatc attgtgaatt ttatattcgg
     6541 attaataaat tctggtaatg taatacgttc atcttcgact ttagcaatgc gtaaaaaatt
     6601 atccataaag aatgatggtt tgataactgt tgcgtttata ttagattcca ttaatctatt
     6661 ttctattttt gctagtactt caaagtgtgg gccagttcga tttcgattaa cccctcccgc
     6721 agtactatac acaatatgtt gaatattttc ttgctcagct atttcaatta tcttcatacc
     6781 ttgtcttaat tcttcgctaa catcatcttt aacgattggc tgaatactgt ataagccata
     6841 cttccctttc atcgctgatt gcaaactatc actatcactc agatcacctt caacgattga
     6901 taaatgcgga tgtcctatgt ctgaaagttt acgattattc ttatttctag ttaatgcact
     6961 tacataccat ccatcctcta acaactgttt tacaactgca ttaccttgct tccctgttgc
     7021 gcctattact aaaatatctt tcattttaga tccactcccc ttttatctta aaaaccaata
     7081 gtcctctttt tcacctttta aatacgtata tggttctggt gtttgtccat tttccttaat
     7141 attgtcattt attacatttc tgacatccca tactaaatga ataaatcgtt gtaattgatg
     7201 actaaaaatg cctagtggcg ctttcatagc taatgctttt tccacatctt ctgtggtaac
     7261 attcagtttt tctgcaatca tttgaaccga taaagttgat gcttcgaaat gtgcttgcaa
     7321 ttcttcagct gtaatacgtc tttgctcttt atttaaagac atacgcaaca ctcttttctt
     7381 ttatgatatt taatagttat tgcaaattca ttgctatctt tcttaacacg ctttataata
     7441 aacggagagt tatccgtttg tcaattttag agaattttaa gtaggtgtaa tcatgcgaaa
     7501 agatgcaaaa gagaataggc aacgaattga agaaatagca cacaaacttt ttgatgaaga
     7561 aggcgttgaa aacattagta tgaatcgtat tgctaaagaa ctaggtatcg gtatggggac
     7621 attgtatcga catttcaaag ataaaagcga tttatgctac tacgtcatac aaagagacct
     7681 tgatattttc atcactcatt ttaagcaaat taaagatgac tatcattctg attatgaggt
     7741 tatgcaagtg tcgttagatt atcttttaca atttaaaatc gataataagg cactactgca
     7801 atgtattgaa gcaggcaaca ataaattacg tttttaccaa agcgcatttt atcaagaatt
     7861 atttaatttt tattatgact tatttaaaag cgatgacgac atttacaaga agttcaaaac
     7921 agatatgctg ttgcaagcat tatcaactag tgtatttgcc ttccaaatag agcatcgcca
     7981 tatttcaatt gaagcgtaca gaaattattt attaaacatt tatttagatg aggtggaacg
     8041 taatgactaa aattgtgtta atcacaggag gcaataaagg gttaggctat gcaagtgcag
     8101 aggcacttaa agcattgggt tacaaagttt atataggttc tcgtaatgac gtaagaggac
     8161 aacaagcatc acaaaaatta ggtgttcatt atgtacagtt agatgttaca agtgactact
     8221 cagtcaaaaa tgcttataac atgattgctg aaaaagaagg ccgtcttgat atcctcatta
     8281 acaatgcagg tatatctggt caattttcag caccttccaa attgacacct cgtgacgttg
     8341 aagaagtata tcaaacgaat gtatttggta tcgttcgaat gatgaacaca ttcgtccctc
     8401 tcttagaaaa atctgaacaa cctgttgtcg tcaacgtatc aagtggttta ggttcatttg
     8461 gaatggttac aaaccccgaa acagctgaat ctaaagtgaa ttcattagct tattgttcgt
     8521 ctaaatcggc agtaacaatg ctaacgctac aatacgctaa aggattacct aacatgcaaa
     8581 tcaatgctgc tgatccaggc gctacaaata ctgatttagt tggtgatttt agcaataatt
     8641 caaaacatgt ttctgaaggg atcaagccaa tcattcaatt agcaaccatt gatgcagatg
     8701 gtccaacagg tacatttatt aatggcgatg gagagatgcc ttggtagatc aattttatgt
     8761 tacaaatatt aaatttagag gtaaataaag aatgcttctg caatagatgc aatatacatg
     8821 caaaaaaata tggaggcgaa ttaaatgaaa agtattacgt ttgaagaaca ttatgtcatt
     8881 gaagatattc aaaaagaaac gatgaatgcg atatcagcag atcctaaagg tgtaccgatg
     8941 aaagtaatgt tagaaggcct tgaaaaaaag accggtttta caaatgccga cgaattatca
     9001 c
//
LOCUS       DAHHOJ010000104         8800 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.104, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000104 DAHHOJ010000000
VERSION     DAHHOJ010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8800)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8800)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8800
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.104"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            90..323
                     /locus_tag="NBH32_002162"
     CDS             90..323
                     /locus_tag="NBH32_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515741.1"
                     /translation="MFYNKYKNVSTYIIIFLVSSAAFAIFLLSANISAHSEQVYEMTD
                     HQIKNNTINKAYEHKDPTNNSEQRDGKVFALIN"
     gene            459..698
                     /locus_tag="NBH32_002163"
     CDS             459..698
                     /locus_tag="NBH32_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 domain-containing protein"
                     /protein_id="HCD1515742.1"
                     /translation="MSNSDLNIERINELAKKKKEVGLTQEEAKEQTALRKAYLESFRK
                     GFKQQIENTKVIDPEGNDVTPEKIKEIQQKRDNKN"
     gene            819..2807
                     /gene="tkt"
                     /locus_tag="NBH32_002164"
     CDS             819..2807
                     /gene="tkt"
                     /locus_tag="NBH32_002164"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001661967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCD1515743.1"
                     /translation="MFNEKDQLAVDTLRALSIDTIEKANSGHPGLPMGAAPMAYTLWT
                     RHLNFNPQSKDYFNRDRFVLSAGHGSALLYSLLHVSGSLELEELKQFRQWGSKTPGHP
                     EYRHTDGVEVTTGPLGQGFAMSVGLALAEDHLAGKFNKEGYNVVDHYTYVLASDGDLM
                     EGISHEAASFAGHNKLSKLVVLYDSNDISLDGELNKAFSENTKARFEAYGWNYLLVKD
                     GNDLEEIDKAITTAKSQEGPTIIEVKTTIGFGSPNKAGTNGVHGAPLGEVERKLTFEN
                     YGLDPEKRFNVSEEVYEIFQNTMLKRANEDESQWNSLLEKYAETYPELAEEFKLAISG
                     KLPKNYKDELPRFELGHNGASRADSGTVIQAISKTVPSFFGGSADLAGSNKSNVNDAT
                     DYSSETPEGKNVWFGVREFAMGAAVNGMAAHGGLHPYGATFFVFSDYLKPALRLSSIM
                     GLNATFIFTHDSIAVGEDGPTHEPIEQLAGLRAIPNMNVIRPADGNETRVAWEVALES
                     ESTPTSLVLTRQNLPVLDVPEDVVEEGVRKGAYTVYGSEETPEFLLLASGSEVSLAVE
                     AAKDLEKQGKSVRVVSMPNWNAFEQQSEEYKESVIPSSVTKRVAIEMASPLGWHKYVG
                     TAGKVIAIDGFGASAPGDLVVEKYGYTKENILNQVMSL"
     gene            3085..3327
                     /locus_tag="NBH32_002165"
     CDS             3085..3327
                     /locus_tag="NBH32_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011275855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HCD1515744.1"
                     /translation="MATWLAIIFIVAALILGLIGGFLLARKYMMDYLKKNPPINEEML
                     RMMMMQMGQKPSQKKINQMMTMMNKNMDQNMKSAKK"
     gene            3314..3412
                     /locus_tag="NBH32_002166"
     CDS             3314..3412
                     /locus_tag="NBH32_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515745.1"
                     /translation="MRKSKFAIDRGYFPDMEMASFYNQINKNKYVY"
     gene            3506..3973
                     /locus_tag="NBH32_002167"
     CDS             3506..3973
                     /locus_tag="NBH32_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001566616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CcdC family protein"
                     /protein_id="HCD1515746.1"
                     /translation="MLYLIFSIIVALFMGTIVIVIRMKAQNYPVNEKKIVLPPFFMAT
                     GALMYVVPYFRLTGSEMLEAFIIGLLFSTVLIWTSRFEVKGTEIYMKRSKAFPVILIS
                     LLIIRTVMKIFISNEIDPGELGGMFFLLAFCMIVPWRAAMLYKYKKLKKTLIN"
     gene            4097..5218
                     /locus_tag="NBH32_002168"
     CDS             4097..5218
                     /locus_tag="NBH32_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HCD1515747.1"
                     /translation="MKIIHTADWHLGKILNGKQLLEDQAYILDMFVEKMKEEEPDIIV
                     IAGDLYDTTYPSKDAIMLLEQAIGKLNLELRIPIIIISGNHDGKERLNYGASWFEHNQ
                     LFIRTDFTSINSPIEINGVNFYTLPYATVSEMKHYFEDDTIETHQQGITRCIETIAPE
                     IDEDAVNILISHLTVQGGKTSDSERPLTIGTVESVQKGVFDIFDYVMLGHLHHPFSIE
                     DDKIKYSGSLLQYSFSEAGQAKGYRRVTINDGIINDVFIPLKPLRQLEIISGEYNDVI
                     NEKVHVKNKDNYLHFKLKNMSHITDPMMSLKQIYPNTLALTNETFNYNEENNAIEISE
                     KDDMSIIEMFYKHITDKELSDIQSKKIKNILENELRKED"
     gene            5222..8251
                     /locus_tag="NBH32_002169"
     CDS             5222..8251
                     /locus_tag="NBH32_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000803170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HCD1515748.1"
                     /translation="MKPLHLKLNNFGPFLKEEIDFSKIDNNELFLISGKTGSGKTMIF
                     DAMTYALFGKASTEQREENDLRSHFADGKQPMSVTFEFQLNHRIYKVHRQGPYIKEGN
                     TTKTNAKFDVFEMVDGKYEIRESKVISGTQFIIELLGVNADQFRQLFILPQGEFKRFL
                     ISNSREKQGILRTLFDSEKFEAIREILKEEVKKEKAQIENRYQQIDLLWQEIESFDDD
                     NIKGLLEVATQQIDKLIENIPLLQARSKEILASVNESKETAIKEFEIIEKKTLENNIL
                     KDNINQLNKNKIDFVQLKEQQPEIEGIEAKLKLLQDITNLLNYIENREKIETKIANSK
                     KDISKTNNKILNLDCDKRNIDKEKKMLEENGDLIESKISFIDKTRVLFNDINKYQQSY
                     LNIERLRTEGEQLGDELNDLIKGLETVEDSIGNNESDYEKIIELNNTITNINNEINII
                     KENEKAKAELDKLLGSKQELENQINEETSILKNLEIKLDRYDKTKLDLNDKESFISEI
                     KSAVNIGDQCPICGNEIQDLGHHIDFDSIAKRQNEIKEIEANIHAIKSNIAVHNSEIK
                     FVNEKISNINIKTQSDFSLEVLNKRLLENENALNNQRDLNKFIEQMKEEKDNLTLQIH
                     NKQLRLNKNESELKLCRDLITEFETLSKYNNITNFEVDYKKYVQDVNQHQELSKEIED
                     KLMQLSQRKSIEQNNLNHYENQLETYNNDLELNEQSIEMEMSRLNLTDDNDIDEIIAW
                     RGEQEELEQKRDTYKKRYHEFEMEIARLESLTKDKELLDSDKLKDEYELKKGKMNTLI
                     DEYSAVHYQCQNNINKTQSIVSHINYLNQELKDQQEIFQLAEIVSGKNNKNLTLENFV
                     LIYYLDQIIAQANLRLATMSDNRYQLIRREAVSHGLSGLEIDVFDLHSNKSRHISSLS
                     GGETFQSSLALALGLSEIVQQQSGGISLESIFIDEGFGTLDQETLETALDTLLNLKST
                     GRMVGIISHVSELKNRIPLVLEVKSDQYQSSTRFKRN"
     gene            complement(8322..8684)
                     /gene="mscL"
                     /locus_tag="NBH32_002170"
     CDS             complement(8322..8684)
                     /gene="mscL"
                     /locus_tag="NBH32_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008530241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HCD1515749.1"
                     /translation="MLKEFKEFALKGNVLDLAIAVVMGAAFNKIISSLVENIIMPLIG
                     KIFGSVDFAKEWSFWGIKYGLFIQSVIDFIIIAFALFIFVKIANTLMKKEEAEEEAVV
                     EENVVLLTEIRDLLREKK"
BASE COUNT         3425 a         1135 c         1543 g         2697 t
ORIGIN      
        1 aaaacaaaca tttgttcgtt aaaatattga cagagaacat aagttctgat aaattactaa
       61 tacaaacaaa tgttctagga gtgaaaatga tgttttacaa taaatataaa aacgtatcaa
      121 catatatcat catattttta gtttcaagtg cagcctttgc aatattcttg ttaagtgcga
      181 acattagtgc tcactcggaa caagtgtacg aaatgactga ccatcaaatt aagaacaata
      241 cgataaataa agcatacgaa cataaagacc ctacaaacaa tagcgaacaa agagatggga
      301 aagtgttcgc tttaataaat tgatacattg tcacaacgtt attttgccta tttttgcgaa
      361 atagcgtttt ttattacttt tttgctgatc ttaaatttgt tatattttgt taaagtatta
      421 taatgattga ataaacaaat tgaaggtagg ttttttaatt gagtaattct gatttgaata
      481 tcgaaagaat taacgagtta gctaaaaaga aaaaagaagt aggattaact caagaagaag
      541 caaaggagca aacagcctta agaaaagctt atcttgagag ttttagaaaa gggtttaaac
      601 aacaaattga aaatactaaa gtaattgatc cagaaggtaa tgatgtaaca cctgaaaaaa
      661 ttaaagagat acaacaaaaa agagataata aaaattaaat cacaaatctg taaagaattt
      721 tctgacatta taacttgaaa taagtatttt acttatcttt ttattttaaa ataagttata
      781 atgtatttga taaaattgaa gaagggaaga tacacaagat gtttaatgaa aaagatcaat
      841 tagctgttga tacgctacgt gcactaagta tcgacacaat cgaaaaagcg aattctggtc
      901 atccaggatt acctatggga gctgccccaa tggcttacac tttgtggaca cgtcatctga
      961 attttaatcc acaatctaaa gattacttca atagagaccg tttcgtatta tctgcagggc
     1021 atggttcagc attattgtat agcttgttac atgtttctgg tagtttagaa ttagaagaat
     1081 taaagcaatt tagacaatgg ggttctaaaa caccaggtca tcctgaatac agacatacag
     1141 atggtgtaga agttactacc ggaccacttg gacaaggttt tgctatgtca gtaggattag
     1201 ctttagcaga agatcaccta gcagggaaat ttaataaaga aggatataat gttgtagatc
     1261 attacacata tgtattagct tctgacggtg atttaatgga aggtatatcg catgaagcag
     1321 cttcatttgc tggacataat aaattaagta aattagttgt tttatacgat tcaaatgata
     1381 tttcattaga tggcgaatta aacaaagctt tttctgaaaa cacaaaagct cgttttgaag
     1441 catatggttg gaattactta ctagttaaag atggtaatga tttagaagaa attgataaag
     1501 cgattactac agctaaatct caagaaggac caacgattat tgaagttaaa acaacaatcg
     1561 gatttggttc accgaataaa gcaggaacta atggtgttca tggggcacct ttaggtgaag
     1621 ttgaaagaaa attaacattc gaaaattacg gtttagatcc tgaaaaacgt tttaatgttt
     1681 cagaagaggt atacgaaatt ttccaaaata ctatgttaaa acgtgctaat gaagatgaat
     1741 ctcaatggaa ttcattatta gaaaaatatg cagaaacata tcctgaatta gcagaagaat
     1801 ttaaattagc gattagtggt aaattgccta aaaattataa ggatgaatta ccacgttttg
     1861 aactgggtca taatggtgca tctcgtgctg attctggtac tgttattcaa gcaatcagta
     1921 aaactgtccc ttcattcttt ggtggatcag cagaccttgc tggttcaaac aaatccaatg
     1981 taaatgatgc aactgattat agttctgaaa cacctgaagg taaaaatgtg tggtttggtg
     2041 tacgtgaatt tgctatgggt gctgctgtaa atggtatggc tgcacatgga ggtttacatc
     2101 catatggtgc aacattcttc gtatttagtg attatttaaa accagcgtta cgtttatcat
     2161 caattatggg attaaatgca acgttcatct tcacacatga ttcaattgca gtaggtgaag
     2221 atggtcctac tcatgaacca attgagcaat tagctggatt aagagccatt ccaaatatga
     2281 atgttatccg tcctgctgat ggtaatgaaa caagagtagc atgggaagtt gccttagaat
     2341 ctgaatctac acctacttca ttagtattga cacgtcaaaa cttaccggta ttagatgtac
     2401 cagaagatgt agttgaagaa ggcgttcgaa aaggtgccta tacagtttat ggctctgaag
     2461 agacaccaga attcctatta ttagcttcag gttcagaagt tagtcttgca gttgaagctg
     2521 ctaaagatct tgaaaaacaa ggtaaatcag tacgtgttgt ttcaatgcct aactggaatg
     2581 catttgaaca acaatctgaa gaatataaag aatcagttat tccatcaagc gtaacaaaac
     2641 gtgttgcgat tgaaatggct tcaccgcttg gatggcataa atatgtaggt actgcaggta
     2701 aagttattgc tattgacggc tttggcgcaa gtgcacctgg cgatttagta gttgaaaaat
     2761 atggatatac aaaagaaaat atcttaaacc aagttatgag cttataagaa taatttataa
     2821 agcgagtatg tttagaagtc taggatgcat aatcttaggc ttcttttaaa gtgttgaaat
     2881 ttagagtata gcacttaaac tacatcataa gtgataagtt atgaaagtat actatttcag
     2941 attaatcttt aaaagctctg ttataacagc atgatttttg atattatttt tagtatcgat
     3001 attaaaatac ttgaataaac tagttcttga aataatgtga tgaatttagt aaaattcagt
     3061 aggataaaga aagcgggtga aacaatggca acttggttag caattatttt tatagtagct
     3121 gcattaattt taggtttaat tggaggtttc cttttagcta gaaaatatat gatggactac
     3181 ttgaagaaaa acccaccaat caacgaagaa atgcttcgta tgatgatgat gcaaatgggt
     3241 caaaaacctt ctcagaagaa aattaatcaa atgatgacga tgatgaataa aaatatggat
     3301 caaaatatga agagtgcgaa aaagtaaatt cgcaattgat agaggctatt ttccagatat
     3361 ggaaatggcc tctttttata atcaaattaa taagaataaa tatgtttatt aaaattaaag
     3421 ttaacaaaat gacgaataga ctgagaaatg ctataattca ttttgtatga tttacagaga
     3481 gtttatttaa cgagaaggtg tctgcgtgct ctatttaata ttttcaatca ttgtagcttt
     3541 atttatggga actatagtta tagttattcg tatgaaagct caaaattatc cggtaaatga
     3601 gaaaaaaata gttttgccac cgttttttat ggcgaccggt gcattgatgt acgtcgttcc
     3661 atattttagg ctaacaggat cggaaatgct agaagccttt ataattggtt tgcttttttc
     3721 tacagttcta atttggactt ctcgatttga agtcaaaggt acagaaattt atatgaaacg
     3781 atctaaagca tttccagtta ttttgatttc attacttatc attcgtactg tgatgaaaat
     3841 attcattagt aatgaaatag atcctggaga attaggcggc atgttctttt tattagcatt
     3901 ctgtatgatt gttccttgga gagcagcaat gctatataaa tacaaaaaac taaagaaaac
     3961 attaatcaat taattacttt taaaaccact tgtgatcgac ttctaaatca gtcaatgagt
     4021 ggttttaatt ttacttgaaa aggtgaaatg attatattaa aataaaagcg aacaaatgtt
     4081 ctatatggag gcagtaatga aaattataca tacagcagac tggcacttag ggaaaatatt
     4141 aaatggcaaa cagcttttag aagatcaagc gtatatttta gatatgttcg tagaaaaaat
     4201 gaaagaagaa gaacctgata tcattgtgat agctggagat ttatatgaca caacatatcc
     4261 aagtaaagat gcaatcatgt tattagaaca agcgattgga aagttaaatt tagaactgcg
     4321 tataccaata attattatta gtggaaatca cgatggtaaa gagaggttaa actatggggc
     4381 gagttggttt gaacataatc agttatttat aagaacagat tttacatcga ttaattcacc
     4441 aatagagata aatggggtta atttttatac actcccttat gctactgtga gcgaaatgaa
     4501 acactacttt gaagatgaca ccattgaaac acatcaacag ggaattacgc gctgtattga
     4561 aacaatagca ccggaaattg atgaagatgc cgtcaatatt ttaattagtc atctgactgt
     4621 tcaaggtgga aagacatctg attctgaaag accattaact attggaacgg ttgaatcagt
     4681 tcagaaaggt gtttttgata tatttgatta tgtcatgcta ggtcacttgc atcatccatt
     4741 tagtatagaa gacgacaaaa ttaaatatag tggctcctta ttgcagtatt cattttcgga
     4801 agcgggtcaa gctaaagggt atagacgtgt aacaattaat gatggcatta ttaacgatgt
     4861 atttattcct cttaagccac ttagacaatt ggaaattatc tcaggcgaat ataatgatgt
     4921 tattaatgaa aaagttcatg tgaaaaataa agataattat ttacatttta aacttaaaaa
     4981 tatgtctcat attactgatc caatgatgag tttaaaacaa atttatccta atactttagc
     5041 gctgacgaat gaaactttta attacaatga agaaaataat gctatagaaa taagtgaaaa
     5101 agatgacatg tcaattatcg aaatgtttta taaacatata actgataaag aattatcgga
     5161 tatccaatct aaaaagataa aaaatatttt agaaaacgaa ttgagaaagg aggattaatg
     5221 aatgaaacca ttacatttaa agttgaataa tttcggcccc tttttaaaag aagaaattga
     5281 tttttctaaa attgataata atgaattgtt tttaataagt ggtaagactg gatcgggtaa
     5341 aacaatgatt tttgatgcaa tgacttatgc cttgtttggt aaagcatcaa ctgaacaaag
     5401 agaagaaaat gatttgagaa gtcatttcgc tgatggtaaa cagccgatgt cagtaacatt
     5461 tgaatttcaa ttaaatcatc gaatttataa agtgcataga caaggccctt atatcaaaga
     5521 aggtaataca acaaaaacga acgctaaatt tgatgtattt gagatggtgg atggcaagta
     5581 tgaaattaga gaaagtaaag taatttcagg tacccaattc attattgaat tattaggagt
     5641 aaatgcagat caattccgac aattgtttat tttgcctcaa ggtgaattca aacgcttttt
     5701 aatatcaaac agtcgtgaaa agcaagggat attaagaaca ctgtttgaca gtgaaaaatt
     5761 tgaagctata cgagaaatat taaaagaaga agtaaaaaaa gaaaaagctc aaatcgagaa
     5821 tagatatcaa caaattgacc ttttatggca agaaattgaa tcatttgatg atgacaatat
     5881 aaaaggctta ttagaggttg ccactcaaca gatagacaaa ttgattgaaa atataccact
     5941 tttacaagct aggtcgaaag aaatactagc atctgtaaat gaaagtaaag aaactgctat
     6001 taaagaattt gaaataatag aaaagaaaac attagaaaat aatatattaa aagataatat
     6061 taatcaactc aacaaaaata aaattgattt cgttcaattg aaagaacaac aacctgaaat
     6121 agagggaatt gaagctaagt taaagttgtt acaagatatt acaaacctat tgaattatat
     6181 tgaaaataga gaaaaaattg aaactaaaat tgctaatagc aaaaaagata tttctaaaac
     6241 caataataaa atattgaatc ttgattgtga taagcgaaac atagacaaag agaaaaaaat
     6301 gttagaagaa aatggagatt taattgaaag taaaatctct tttattgata aaactagagt
     6361 attatttaac gatattaata agtatcaaca aagttatctc aatattgaac gcttgagaac
     6421 tgagggtgaa caattaggtg atgaattaaa tgatctaatt aaaggtttag aaacggtcga
     6481 agattcaata ggtaataacg aaagtgatta cgagaaaatt atcgaactaa ataatacgat
     6541 aacgaacata aataatgaaa ttaatataat taaagaaaat gaaaaagcta aagctgaatt
     6601 agataaacta ttaggtagta agcaagagtt agagaatcaa attaatgaag aaacatctat
     6661 attgaagaat ctcgaaataa aattagatcg ctacgataaa acaaaattgg acttaaatga
     6721 taaagaaagc tttataagtg aaattaaatc tgctgtaaat attggagatc aatgtccgat
     6781 atgtggtaat gaaattcagg atttagggca tcatattgat tttgacagta ttgctaaacg
     6841 tcaaaatgaa attaaagaaa ttgaagcaaa tatccacgct ataaaatcga atattgctgt
     6901 gcataattct gaaattaaat ttgttaatga aaaaatatcg aatattaata ttaaaacgca
     6961 aagtgatttt tcacttgaag tattgaataa gcgtctgcta gaaaatgaaa atgcattgaa
     7021 taatcaaaga gatcttaata aatttataga acaaatgaaa gaagaaaaag ataatctaac
     7081 gttgcaaatt cataataaac aattgcgtct aaataaaaat gaatctgagt tgaaattatg
     7141 tcgagatctc atcactgaat ttgaaacact ctcgaaatat aataatatca ctaattttga
     7201 ggtggattat aagaagtatg ttcaagatgt gaatcagcat caagaactct caaaggagat
     7261 tgaagataag ttaatgcaat tgtctcaaag aaagtcaatt gagcaaaata atctaaatca
     7321 ttatgaaaat caactagaaa cttacaataa tgacttagaa ttgaatgaac agtctattga
     7381 aatggaaatg tcgaggctga atttaactga tgacaatgat atagatgaaa taatagcctg
     7441 gaggggcgag caagaggaat tagagcagaa aagggatact tataaaaaac gttatcatga
     7501 atttgaaatg gaaatagcta ggttagaatc attaactaag gataaagagt tattggactc
     7561 tgataaatta aaagatgaat atgagctaaa aaaaggaaag atgaatacac tgatagatga
     7621 atactctgct gttcattatc aatgtcaaaa taatattaat aaaacacaat ctatagtttc
     7681 gcatattaat tacttaaatc aagaattaaa ggatcaacaa gaaatatttc aattggctga
     7741 aattgtcagt ggtaagaata acaaaaatct tacattggaa aactttgtct taatttacta
     7801 tttagatcaa attattgctc aagcaaatct gagattagca acaatgtcag ataatcgata
     7861 ccaactaatt aggcgagaag cggtttctca tggtcttagt ggcctagaaa ttgatgtatt
     7921 tgatttgcat tcaaataagt ctagacatat tagctcgtta tcaggtggag aaactttcca
     7981 atcgtcgctt gcattagctt tagggttaag cgaaattgta cagcagcaat caggaggtat
     8041 ttcactagaa tcaatattta ttgatgaagg attcggtaca ttagatcaag aaacgcttga
     8101 aacagcgtta gacactttat taaatcttaa atcaactggt agaatggttg ggattatttc
     8161 acatgtgagc gaattgaaaa atagaatacc tttagtttta gaagtgaaat cagatcaata
     8221 tcagagttca acaagattca aaagaaatta aaacataaaa aatagagatg gtactttgga
     8281 atgtatagaa caaccttagc ccatctcttt ttatttaaaa attatttttt ctcacgtaat
     8341 aaatctctga tttcagttaa taacacaaca ttttcttcca caactgcttc ttcttcggct
     8401 tcttctttct tcattaatgt atttgcaatc ttaacaaaga tgaataaagc aaacgcgatg
     8461 ataataaagt cgataacaga ttggataaat aaaccgtatt taatacccca gaatgaccat
     8521 tctttagcaa aatcaactga tccgaaaatt ttaccaatta atggcataat gatattttct
     8581 actaatgaag atataatctt gttgaaagct gcacccatca caacagcaat tgctaaatct
     8641 aagacgttac cttttaaggc gaactctttg aattctttta acattacact caacctctct
     8701 tttttaaatg taacctcaat tatacaacaa ataaaaaaat tagaaaatag attattcaag
     8761 aaaatttgat tatcgcaaag acctttgtta tcctataaag
//
LOCUS       DAHHOJ010000105         8711 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.105, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000105 DAHHOJ010000000
VERSION     DAHHOJ010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8711)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8711)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8711
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.105"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(132..839)
                     /locus_tag="NBH32_002171"
     CDS             complement(132..839)
                     /locus_tag="NBH32_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000667076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515750.1"
                     /translation="MKFDLIISNIMIGILVICSAFALMFVFYIQRKLTPEQYFSFSEF
                     FLIGRGLTIKKILNRMILIASFNCILFLILKNFFEIELVCSVCILSTLLGSILIIYPA
                     FKIRHDLPNNEMKSLLNYMYVSFVFLALIISTLTVFTLVVLFDDISIVTLWQEHKSGI
                     ILYLLLVIPSLFFNPTNYEKKMEYAIEKHRESNREINETIIKNNNETIIPDESKSSSP
                     FKIKKVFRLLNRIMKDD"
     gene            complement(952..4080)
                     /locus_tag="NBH32_002172"
     CDS             complement(952..4080)
                     /locus_tag="NBH32_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HCD1515751.1"
                     /translation="MIFQFSEDDLEQVALKWFEGLGYSVKNGRDSSDNGIMNERESDK
                     DVVLDDRLEAALRRINPELNNSAIEQAIHEISIEKSPSLLENNRSFHEMITNGIEVEH
                     YNDDGETINDLVYVLDFDNPGNNDFLAVNQLTVVNGDYKKRPDIVLFINGLPVVVIEL
                     KNSTNESVGIEDGYHQLETYKMRIPQLFNHNAVLVTSDGVNTKAGSLTADYDRFMTWR
                     TKDGKTEDSSTFRSLDILIHGMLNKEVLLDLIRHFVLFQDDGKGNIVKILAAYHQYYA
                     VNKAVDRAMEATSEHGDGKGGVIWHTQGSGKSLTMVFFSGKLIQKLNNPTLVVVTDRN
                     DLDNQLYGTFVKSKGRSGKGLLRQTPKQAESRKELKELLSVESGGIVFTTMQKFEPEE
                     GQVTMDALTERKNVVVMADEAHRTQYGFKATYNEKGEGIKYGYAKYLRDALPNASFVG
                     FTGTPVSSTDKNTQMVFGNYIDVYDMTQSVEDGSTVKIYYESRIIPLNLPTDLDIDDA
                     YDVITFGQEEDTKSRLKSKWSRIEALSGAEKRVHALATEIINHFETRQKAMNGKGMIV
                     TMSRRIAVDLYDEIIRLKPEWHSDDDDKGVIKVVMTGSSSDPQNFQKHIGPKKRRNLL
                     ENRMKDINDELQLVIVRDMWLTGFDVPSMHTMYIDKPMKGHNLMQAIARVNRVFKDKP
                     GGLIVDYVGIAESLKEALKEYTDSDREQTGIDTDKALELMLVKYDIIQDMLYNHDYSD
                     FESEDQLNRYNAISNTMDYIIGLGEKERERFVTTVTELSKAFALCATEEAAQELNSEI
                     AFLKAVKSGLVKLLAPPNEGSKLKKTPAEIEAEINQLISKSVVTEEVVDIYQTLGIEN
                     PDISILSDDFLKDVEGLQQKNVAVELLNKLLKGQVKSLMKTNTTVSKRFSEMLSKSIQ
                     KYNNRSIEASKVIEELIQMAKEINQEKERGKELGLSTEEIAFYDALASHETAKEAMDD
                     KELRAIAHELTKTVKNNMSVDWSKRESAKAKMRVQVRRLLKKYGYPPDIQKMAVEQVV
                     EQAELMASQQYK"
     gene            complement(4064..5296)
                     /locus_tag="NBH32_002173"
     CDS             complement(4064..5296)
                     /locus_tag="NBH32_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000394000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1515752.1"
                     /translation="MEFETFNLTDLYTISSGLSKNRKYFGTGTPFLTFKDVFDNLILP
                     NEFSGQVITEEKEREKYSVKKGDLFLTRTSEKQNELGISAVALKDYKNATFNGFTKRL
                     RPNKYCENKLLPVFAAFYFRSNNFRNQVNSMSIMSTRASLNNEMISKLKITIPSLQNQ
                     MKISHILLALLKKEKINQKIIANLEELSQTLFKRWFVDFEFPDENGNPYKSSGGEMVD
                     SELGEIPRSWKVDELGNYIKIKSGKRPKNKVDKEDIENVVPIIGASKIVGYTNDYLYN
                     EKIIIIGRVGTHGVIQRFSTRTWPSDNTFVITSDFESIIYQVLKSIDYISLNRGSTQP
                     LLSQKDIKNTKVVMPTNATLLSKYQKKNNHILKMMDQKIIENKKLTQLRDTLLPKLMS
                     GEIEIPDDIEVNEDDLSI"
     gene            complement(5286..6800)
                     /locus_tag="NBH32_002174"
     CDS             complement(5286..6800)
                     /locus_tag="NBH32_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017175164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent DNA methyltransferase"
                     /protein_id="HCD1515753.1"
                     /translation="MATIGFEEKLWQAADKLRGSMDAAEYKNVALGIIFLKYVSDSFE
                     EKYEELLNDEYADEEDKDEYLAENIFWVPKESRWQYINDNSKKPEIGQIIDKAMIAIE
                     RENESLKGVLPKDYARPALDKEKLGDIIDLFTFKVGDSESKKQDILGRVYEYFIAKFA
                     SAEGKNAGEFYTPASIVKLLVEMIEPYQGRIYDPCCGSGGMFVQSEHFIERHQGRIDD
                     IAVYGQESNPTTWKLAKMNLAIRGIDNNLGDHHADTFHNDLHKDLKADFILANPPFNA
                     SDWGREKLQDDYRWKFGIPPKGNANYAWIEHMISKIAPSGTAGFVLANGSMSTSGKDE
                     LEIRKNLIEQDMVECIVTLPGQLFYSTQIPVCLWFMTKNKAKNGKNERRGEVLFIDAR
                     NIGSMVSRTLKEFSDDEIKDIANVYHSWRGTNNNQYEDKAGFCKVAKTEEIKNNEYIL
                     TPGRYVGLAEVEEDSEPFEQKMERITADLSEQFAKSKELEDQIRKSLEGLGYGV"
     gene            complement(6839..7324)
                     /locus_tag="NBH32_002175"
     CDS             complement(6839..7324)
                     /locus_tag="NBH32_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000073145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515754.1"
                     /translation="MSNVKKDEYIKLHQSYLNEYIKFADAKALAIISINGFILNFNFS
                     KRNIKFHNTEDIFNFTAFILLIITIILAAFAVYPRTNNRSEKGIIFWDNINSMGEKEF
                     IEKVKFEKEEELLEKTIQQNYFLAKTASMKYSIIRKVFIISALGYSCLLFSSIFQIIC
                     S"
     gene            complement(7321..8592)
                     /locus_tag="NBH32_002176"
     CDS             complement(7321..8592)
                     /locus_tag="NBH32_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HCD1515755.1"
                     /translation="MDNHIRIFDNLFQSNISKFQNLTSKSYIIRNDNEKNSYLPMVQE
                     IRELFGKEGEIFSKSIGTHPDFFGIENTNEYQYICSLFVDISGSTKLALKYSLDKVKL
                     YKNAIISSAIEIFRAFDGHIHRIQGDAVLVYFGHKELEKSDAIINAINAASLMQYFNA
                     TTLKKFFESENLEPLKIRIGIDFGDDSSVLWSKYGIDGINEITSTSIHTDLASKFQNK
                     APSNKIMIGENINKYLDIPKKFRSIKIEKNNGVDVEKRYILNTNNLGRYSMEVFEWEK
                     YLNSFSMLPPFSTENEQFYSPRDLKIRCWIIDEKNQDKYEYIERGSALKKEMNLLFKL
                     EIYNQCLEFKNIKWRVVNYGEEAKKDKELEFEMNQYEGYQYCNQKTAYTGLHFMECYL
                     YDINDKIICHDSFGLFINDNNREVRKLGIED"
BASE COUNT         2689 a         1625 c         1018 g         3379 t
ORIGIN      
        1 aacctctatg catgttctca atacaataaa tataacttaa catatcaaag ttatcattaa
       61 aattcatttt taaatgtaaa atttcgcatt tattattttc aaataaatgt ataattttct
      121 tcaaataata ttcaatcatc tttcataatc ctattcaaca atctaaaaac ttttttaatt
      181 ttgaatggtg aagaactttt cgactcgtct ggtataattg tttcattatt attttttatt
      241 atcgtctcat ttatctctct attactctca cgatgttttt caatggcata ttccattttc
      301 ttttcataat ttgtaggatt aaaaaataga ctaggtatca ccaatagaag atataaaatt
      361 attccacttt tatgctcttg ccaaagtgtt actatactaa tatcatcaaa taatactact
      421 aaagtaaata cagtaagagt agaaataata agcgctaaaa aaacaaaaga tacatacata
      481 tagttcaaca aacttttcat ctcattgtta ggtaaatcat gcctaatttt aaacgccgga
      541 taaataatta aaatcgaacc caacaatgta cttagtatac acacactaca tactaattca
      601 atttcaaaaa aattcttaag tattaaaaac aaaatacagt taaaactagc aatcaatatc
      661 attctattca aaattttctt gatagttaaa cctcttccta ttaaaaagaa ttctgaaaaa
      721 gaaaagtatt gttccggcgt taactttctt tgaatataaa agacaaacat taaggcaaat
      781 gcagaacaaa tcactaatat ccctatcata atattactta taattaagtc gaatttcata
      841 tcaatctcct tttctctata gtctaccggt atattgacac ttttttatat actctgtctt
      901 aaatgtttaa ttttaaaaaa gtatctaaca ggtcaacaat ataaacagta tttatttata
      961 ttgttgactt gccattaatt ctgcttgttc cactacttgt tctactgcca ttttttgtat
     1021 gtctggtgga taaccatatt tctttaatag tcgtcttact tggactctca ttttagcctt
     1081 tgcgctctca cgttttgacc agtctacgct catattattc ttaaccgttt ttgttaattc
     1141 atgtgcaata gcccttaatt ctttatcatc catcgcttct tttgctgttt catgtgaagc
     1201 taatgcgtca taaaatgcaa tttcttcagt acttaaacct aattcttttc ctctttcttt
     1261 ttcttgattt atttctttcg ccatttgaat taattcttct atgacttttg atgcttcgat
     1321 agaacggtta ttatattttt gtattgattt acttagcatc tcagaaaacc ttttagatac
     1381 agtagtatta gttttcatta atgacttaac ttgtcctttg agtaatttgt tcaacaattc
     1441 aactgcaaca tttttttgct gtagtccttc tacatctttt aagaaatcat ccgacaatat
     1501 tgagatatct ggattttcaa ttccgagtgt ttgataaata tctacaactt cttcagttac
     1561 aactgacttt gaaatcaatt gatttatttc tgcttctatt tcagcaggtg tctttttaag
     1621 cttgcttcct tcattaggtg gagctaataa tttaacaagt cctgatttta ctgcttttaa
     1681 aaacgcaatt tcactattta gttcttgagc agcttcctct gttgcacata atgcaaaagc
     1741 cttagaaagt tctgtaactg ttgtcacaaa tcgctctctt tctttctcac ctaatccaat
     1801 gatataatcc atcgtatttg agatagcatt atagcgattt aattgatctt ctgactcaaa
     1861 gtcagaatag tcatggttat atagcatatc ttgaatgata tcgtatttca ctaacattag
     1921 ttctagcgct ttatctgtat ctatacctgt ttgttctcta tcagagtccg tatactcttt
     1981 tagtgcttcc ttcaggcttt cagcaatacc tacatagtca acaattaagc cacctggttt
     2041 atctttaaat actctattaa cacgtgcaat agcttgcatt aaattatgac ctttcatcgg
     2101 tttatctatg tacattgtat gcattgaagg tacatcaaat cctgttaacc acatgtctcg
     2161 cacaattacg agttgcagtt catcattaat atctttcata cgattctcta aaaggtttct
     2221 tctctttttc ggacctatat gtttttggaa gttttgcgga tcacttgatg aacctgtcat
     2281 aacgacttta attacacctt tgtcgtcatc atcagaatgc cattcaggtt taagtcttat
     2341 aatttcatca tataaatcaa ctgcaatacg acgactcatt gtgacaatca tacctttacc
     2401 gttcattgcc ttctgtcttg tttcaaagtg attaataatt tcagtcgcta aagcatgtac
     2461 tcgtttttct gcaccagata gagcttctat acgtgaccat ttagatttca atcgagattt
     2521 agtatcttct tcttggccaa aagtaatgac atcatatgca tcatcaatat ctaaatcagt
     2581 tggtaaattt aatggaataa tacgactttc atagtatatt ttaaccgtac taccgtcttc
     2641 tacagactgt gtcatatcat atacatcaat ataattacca aataccattt gcgtattctt
     2701 atctgtcgaa gatacaggtg ttccagtaaa accaacgaac gaagcgttag gtaaagcgtc
     2761 tcttaaatat tttgcataac catattttat accttcacct ttttcattgt aagttgcttt
     2821 aaaaccatat tgtgtgcggt gtgcttcgtc tgccataact actacatttt tacgttctgt
     2881 taaagcatcc attgtcactt gaccttcttc aggttcgaat ttctgcatag ttgtaaatac
     2941 aataccacct gattctacag ataatagttc tttcaattct tttcgtgatt ctgcttgttt
     3001 aggtgtttgt cttagtaatc ctttacctga gcgtcctttg gacttaacaa aagtaccata
     3061 taattgattg tctaagtcat tacggtctgt tacgaccact aatgttggat tatttaattt
     3121 ttgaattaac tttcctgaga agaagaccat tgtcaaactc ttaccagaac cttgtgtgtg
     3181 ccaaataacg ccgcccttac catcaccatg ttcagatgtt gcttccatag cacgatcaac
     3241 agctttatta accgcataat attgatgata tgcagctaat attttaacta tatttccttt
     3301 accatcatct tggaacaaaa caaaatgacg aattaaatct aatagtacct ctttatttaa
     3361 cataccgtga atcaatatat ctaaactacg aaacgttgat gaatcttcag tttttccatc
     3421 tttagtacgc caagtcataa aacggtcata atcagcagtt aaagaaccag cttttgtatt
     3481 gacaccatca ctcgttacta atactgcatt atgattaaat aattgtggaa ttctcatttt
     3541 ataagtttct aactgatgat acccgtcttc aataccaaca gattcatttg tagaattttt
     3601 cagttcaatt accactactg gtaatccatt tatgaataac acaatatcag gtcttttttt
     3661 ataatctcca tttacaacag ttaattgatt aaccgctaag aaatcattat ttcctgggtt
     3721 atcaaagtca agcacataga ccaaatcatt aatcgtctca ccatcatcat tatagtgttc
     3781 aacctcgata ccattagtaa tcatttcatg gaaagaacga ttattctcta ataaactagg
     3841 agacttctca atagaaattt catgaattgc ctgttcaatt gcactattgt ttagttcagg
     3901 attaatgcgt ctcaaagctg cttccaaacg atcatcaagc acaacatctt tgtcgctctc
     3961 ccgttcattc ataatcccat tgtcactact atctcgacca ttcttaacag aatagccaag
     4021 accctcaaac cacttcagtg caacctgttc taaatcatct tcactaaatt gaaagatcat
     4081 cttcattcac ctcgatgtca tcaggtatct caatttcacc tgacattaat tttggtaata
     4141 atgtgtcacg gagttgggtt aatttcttgt tttctatgat tttttgatcc atcattttta
     4201 aaatatgatt gtttttcttt tgatattttg aaagcaaagt tgcattggtg ggcattacta
     4261 ctttagtatt cttaatatct ttttgagata atagaggttg tgtagaacct ctattcaatg
     4321 atatatagtc tatactttta agtacttgat atataatgct ttcaaaatca cttgttatta
     4381 caaaagtatt atctgatggc catgttcttg tagaaaacct ttggattaca ccatgagttc
     4441 ctaccctacc aattataata attttttcat tatataaata atcattcgta taacccacta
     4501 ttttactagc accaattatt ggcaccacat tttctatatc ttctttatca actttatttt
     4561 taggtctttt accacttttt atttttatat aatttcctaa ttcatcaact ttccaactcc
     4621 taggtatttc acccaattca ctatcaacca tctctccacc gcttgatttg tatggatttc
     4681 cattttcatc aggaaattca aaatcaacaa accaacgttt gaatagtgtt tgagaaagtt
     4741 cttcaagatt tgctatgatt ttttgattga ttttttcttt ttttaaaagt gctaatagta
     4801 tatgactaat tttcatctga ttttgaagac taggtatagt aattttaagt tttgaaatca
     4861 tttcgttatt taaacttgct cttgtagaca tgatagacat agaatttact tgatttctaa
     4921 aattattact tctaaaataa aatgcagcaa atactggtaa caatttattc tcacaatatt
     4981 tgtttggtct taatcttttg gtaaatccat taaaagtcgc atttttatag tcttttaaag
     5041 ctactgcact tatacctaac tcattttgtt tctcactagt tcttgttaaa aaaaggtctc
     5101 cttttttaac tgaatacttt tctctttctt tttcttcagt tataacttgc ccagaaaact
     5161 cattaggtaa tattaaatta tcgaatacat ctttaaatgt taaaaaaggt gtaccagtac
     5221 caaaatattt tctatttttt gataaacctg aactaatagt atataaatca gttaaattaa
     5281 atgtttcaaa ctccataacc taatccctcc aatgacttac ggatttggtc ctcgagttct
     5341 tttgatttcg caaattgttc acttaaatct gctgtgattc tttccatttt ctgttcaaat
     5401 ggttctgagt cttcttctac ttctgctaat cctacataac gtcccggcgt taagatatat
     5461 tcattgtttt ttatttcttc tgtctttgct actttacaga aacctgcttt atcttcatat
     5521 tggttattgt ttgttccacg ccatgagtga taaacatttg ctatatcttt tatttcatca
     5581 tctgagaatt cttttaatgt tcttgatacc atgcttccta tatttctagc atctataaat
     5641 agtacttctc cacgtcgttc attcttgcca tttttagctt tattttttgt catgaaccac
     5701 aaacatactg gaatttgagt tgaatagaat agttgccctg gtaatgtgac aatacactca
     5761 accatgtctt gttcaattaa gtttttacgt atttctaatt catctttacc acttgtagac
     5821 atcgaaccat tcgctaaaac aaatcctgct gtaccacttg gggcaatttt agaaatcata
     5881 tgttcaatcc atgcatagtt ggcattgcct ttaggtggaa taccaaattt ccagcggtaa
     5941 tcatcttgta atttttctcg tccccaatca cttgcattga atggtggatt agccaatata
     6001 aaatcagctt tcaaatcttt atgtaaatca ttatgaaatg tgtctgcatg atgatcacct
     6061 aaattattat ctatacctcg aatagccaaa ttcattttcg ctaatttcca agtagtcgga
     6121 ttagattctt gaccataaac agcaatatca tctatacgac cttgatgacg ttcgataaaa
     6181 tgttcactct gtacaaacat tccgcccgac ccacaacaag ggtcataaat tctaccttga
     6241 tatggttcaa tcatttcaac aagcaattta actattgaag ctggtgtata gaattcccca
     6301 gcattcttac cctcagcgct tgcaaatttt gcgataaagt attcataaac acgacctaaa
     6361 atatcttgct tcttactttc agaatctcca actttgaatg taaataggtc aataatatcg
     6421 cctaattttt ctttatctaa tgcaggtctt gcgtaatctt taggtaatac gccttttaaa
     6481 gactcatttt ctctttcaat tgctatcatt gctttatcga taatttgacc aatttctggt
     6541 ttcttagaat tatcatttat gtattgccat cttgattctt taggaaccca gaaaatattt
     6601 tctgctaagt attcatcttt atcttcttca tcggcatatt catcatttaa taattcttca
     6661 tacttttcct caaaagaatc agaaacatat tttaaaaata taatacctaa tgctacattt
     6721 ttatattctg ctgcatccat acttccacgt aacttatccg ccgcttgcca taacttttct
     6781 tcaaatccaa ttgttgccat atttttattt ttctcctttt catgttaaaa ctgaaaattc
     6841 aagagcatat aatctgaaag atacttgaaa ataataaaca ggaataacct aatgctgaaa
     6901 ttataaaaac ctttctaatg attgaatatt tcatagatgc agtttttgct aaaaaataat
     6961 tttgttgtat cgtcttttca agtaattctt cctctttttc aaatttcact ttctctataa
     7021 attctttttc tcccatacta tttatattat cccaaaaaat aattcctttt tcgcttctat
     7081 tatttgttct tggatatact gcaaatgctg ctagtataat tgtaattatc aataatataa
     7141 aagctgtaaa attgaaaatg tcttcagtat tatgaaattt aatattacgt tttgaaaagt
     7201 taaaatttaa aataaagcca ttaatactta tgatagctaa tgccttggca tcagcaaatt
     7261 taatatattc atttagatag ctttgatgta actttatgta ctcatctttt ttcacattac
     7321 tcaatcctct attcctaatt ttctcacttc tctattatta tcatttataa aaagaccaaa
     7381 actatcatga caaattattt tatcattgat gtcgtacaaa taacattcca taaaatgtaa
     7441 tcctgtataa gctgtctttt gattacaata ctgataccct tcatactgat tcatttcaaa
     7501 ttctaattcc ttgtcctttt ttgcctcctc tccgtagttt accactctcc atttaatatt
     7561 tttaaattct agacattgat tataaatttc taatttaaat aacaaattca tttctttttt
     7621 taaagcacta cctctttcaa tatattcata tttatcctga tttttttcat caataatcca
     7681 acatcttatt ttcaaatctc taggtgagta aaattgttca ttttctgtag aaaaaggagg
     7741 caacattgaa aaactattca agtatttttc ccattcaaac acttccatag aatatcttcc
     7801 cagattattt gtatttaaaa tatatctttt ctcaacatca acaccattgt tcttctcaat
     7861 ttttatactt ctaaactttt taggaatatc aagatattta tttatatttt ctccaatcat
     7921 aatcttattt gaaggggctt tgttttggaa ttttgatgct aagtcagtat gtatactggt
     7981 agaagttatt tcattaattc catcaattcc atattttgac cacaatacac tagaatcatc
     8041 accaaaatca attcctattc taatctttaa cggctctaaa ttttcacttt caaaaaactt
     8101 ttttaatgtt gtggcgttaa aatactgcat taatgacgca gcgttgattg cattaattat
     8161 tgcatcactt ttttccagtt ctttatgtcc aaaataaaca agcaccgcat ctccttgtat
     8221 tctatgaata tgaccgtcaa atgcacgaaa tatttctata gcactagaaa ttatcgcatt
     8281 tttataaagt ttaactttat ccaaactata tttcaaagct agctttgtag acccagatat
     8341 atctacaaac agggaacaaa tgtactggta ttcattagta ttttctattc caaaaaagtc
     8401 aggatgtgtt cctatacttt tagaaaaaat ttctccctct tttccaaaca actctcttat
     8461 ctcttgaacc ataggaagat aactattctt ttcattatca tttcttataa tgtatgattt
     8521 actagtaagg ttttggaatt ttgaaatatt actttgaaat aaattatcaa aaattcttat
     8581 gtgattgtcc attctgtcac ctctttattt attaaattgg ctaatactac ccctacttat
     8641 gatacgcctc tgcgtatcgg ttgataatga ggttttataa attctaacat ttaaacaact
     8701 aaaaattata a
//
LOCUS       DAHHOJ010000106         8638 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.106, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000106 DAHHOJ010000000
VERSION     DAHHOJ010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.106"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..872)
                     /locus_tag="NBH32_002177"
     CDS             complement(<1..872)
                     /locus_tag="NBH32_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1515756.1"
                     /translation="MSIPINLPTNSTMINELCTLQSRTINIKGEVLITEIYDDYFLKN
                     DEWHISAFNKFEQFQDSIKNYKDKRKNIFFRIKSENLNLEFKYLFLKLIVKEDWSLSN
                     VFNTGAVKLNKIAEFFNEVYPNLNSLLDCDINMLEKYWFSWLTKNNIPIKRKSSTIVF
                     GDYEHKSGLASFLKNMYINLNKFIDKREEWEKDKWDIRNLEKYGLSYNKTLTGNYLNF
                     EKIESIKMRELAKKYLKNRLITGDIAFATARFYIRVLTRFFQNISKNKETRNSLNELD
                     RCHIEAYIEFLFEYA"
     gene            complement(875..1987)
                     /locus_tag="NBH32_002178"
     CDS             complement(875..1987)
                     /locus_tag="NBH32_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002485872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1515757.1"
                     /translation="MKIVEVKSKNGINFMILDGNNEPIVDAVRYLKYLDSVNKSLNTK
                     KTYAYALKKFFVYLESKKICYKEVSFDNFVDFIRWMKTPFEYENVLSYHRKGKSISPK
                     TINLTITVVSNFYDYLYRSKKLDVNFYDFIHTESKYSKKYKSFMHHVNKDYGMLKNIL
                     KVKEPKKKLEVLTNVEVKKLLGAANNIRDKFLIQLLYETGLRIGEVLSLRIDDIKFDF
                     RKGHQIVLKNRFNSNGTYLKTGERKIFISQSLIDLYDDYVYEIIDELSICSDYLFVKI
                     KGRNVGEAMHYSDIYSLFKRLKHKTSINVHPHLFRHTHATVFYNETKDIKQVQERLGH
                     SNIQTTINLYVHPTEEDIREDWNKVKHQFQVFNKGE"
     gene            2171..2476
                     /locus_tag="NBH32_002179"
                     /pseudo
     CDS             2171..2476
                     /locus_tag="NBH32_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500634.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
     gene            complement(2533..2706)
                     /locus_tag="NBH32_002180"
     CDS             complement(2533..2706)
                     /locus_tag="NBH32_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515758.1"
                     /translation="MWNFIKCVFKFVFSLVAITTLVAGVGVVAFAYIFKKDFEDIERK
                     TKEIISDIESKNN"
     gene            3157..4197
                     /locus_tag="NBH32_002181"
     CDS             3157..4197
                     /locus_tag="NBH32_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HCD1515759.1"
                     /translation="MQQVTSDIMTFRGSHFELGVKTGKWLQQTPLLKNREKEWKKRVP
                     RFDIDVNETYQIFQTYAPQIWEELMGLQSILKMPTRQIILNFGHYRFTDLKESGCTVF
                     QGKDFMVRNYDYHPATYDGRYLLYQPTDSGLAQIGPVSRVTGRMDGMNESGLTMGYNF
                     MHRKKPANGFVCYMIGRLILENCRNVTEAIQLLKEIPHRSSFSYILMDKSLNHAIVEV
                     TPRSIDVRYDNICTNHFEILTHENRNYTKESKERLARTISQTNDNLDMTTAFKLFNNP
                     QYEIYSKLFKSWSGTIHTSMYHPETLTAYFTLGENAPPEIIDFKSWLDGQELNITHFT
                     GNIDTDLTFANK"
     gene            complement(4254..5096)
                     /locus_tag="NBH32_002182"
     CDS             complement(4254..5096)
                     /locus_tag="NBH32_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HCD1515760.1"
                     /translation="MKKFFFIGLLVFVVFFTAATIIWFSYDKNKYGTKQYDKTFKDDA
                     FDNVSINLDSTELRIKRGNQFRVKYDGDNDILINIVDKTLKISDKRSKTRGYAIDMNP
                     FHENKKTLTIEMPDKMIKRLNISSGAGSVRISDVDLENTSIQSINGEVVIKNSNLDAL
                     DSKTNNSSTYISKSNIKNSNIKVVIGTLQIDKSQIKQSIFLNDHGDIEFKNMPSKVDA
                     KASTKQGDIRFKYDSKPEDTILKLNPGTGDSVVKNKTFTNGKVGKSDNVLEFYTIDGN
                     IKVE"
     gene            complement(5093..5650)
                     /locus_tag="NBH32_002183"
     CDS             complement(5093..5650)
                     /locus_tag="NBH32_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001669025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HCD1515761.1"
                     /translation="MNKITYLNELETALDALPRHLRDQKMYEYERYFYEQELNGVDEE
                     EILKKLKDPQDVAAETKARSVIDYAESKPTFENISRAVAASLSLGILSIFVILIPVSI
                     VGLFVLALFLISLLLLFCPIILLASAISRGIVDSISNVFFAISYSGLGLVFIIVIFKI
                     LEYIYRLILKYLLWYIKTVKGSVRK"
     gene            complement(5710..6234)
                     /locus_tag="NBH32_002184"
     CDS             complement(5710..6234)
                     /locus_tag="NBH32_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515762.1"
                     /translation="MLYLNKYKERVTSVRIQNRWVVFILFLICSFGVLIGLYQYRHTK
                     TVDLSNLEINDIKLNEKFDKKGYEVNKKIKFDRFKFYNSKAHPDLTVKVREKDNIVKG
                     IILVRDEKIHTNFDGGIGSPINNAIENLGFGYKRTKVGNDFSSVKYIDRDNHLKLNLL
                     YQDLEIKRIEFFSK"
     gene            6355..6918
                     /locus_tag="NBH32_002185"
     CDS             6355..6918
                     /locus_tag="NBH32_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1515763.1"
                     /translation="MTNLETYFKNSQPLNEYIDGMKTNQDTVLSIYQSFELPEDDSRI
                     KKIKEMNYSKVLVITEDWCGDAMMNLPILKHISEALNLEVRVFHRDDDTKLIDQYLTN
                     GKSRAIPIFVFLNDQFEQETVWGPRANEVQKFVTDVRSDKLPSKDHPDYADLEKETHL
                     VISNRYKTDASFWKAVYNSILNKLENK"
     gene            complement(6984..7724)
                     /gene="pmtD"
                     /locus_tag="NBH32_002186"
     CDS             complement(6984..7724)
                     /gene="pmtD"
                     /locus_tag="NBH32_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtD"
                     /protein_id="HCD1515764.1"
                     /translation="MRILNLVKYDFYSIFKSPLTYLAILVVSSLIATQSILMANSMDN
                     PKHIIVYGSVFAAAKWLLLIIGLMFVVKTITRDFSQGTIQLYMSKVKTRVGYIISKTI
                     SIILISILFAVIHYVILIVVQASSNGKNLAFSKYVDNLWFFLIFLLFFGLFLFLITLA
                     SQKTAMIFSLGVFLVLIVPFIKPFITFIPRYGEKVLDAFDYIPFAYLTDKMISSNFDF
                     SNWQWVISLGSIVIFFILNILYVAKKDI"
     gene            complement(7724..8596)
                     /gene="pmtC"
                     /locus_tag="NBH32_002187"
     CDS             complement(7724..8596)
                     /gene="pmtC"
                     /locus_tag="NBH32_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtC"
                     /protein_id="HCD1515765.1"
                     /translation="MKLEHITKKYGSNVVLNDIDFDFGDSRIVGLIGKNGVGKTTVMK
                     VMNGNIIKFDGKVDIDNADNIGFLIEHPKLYDNKSGLYNLKLFAQVLGKGFDKAYTDK
                     IIDAFGMRPYIKKKVKKYSMGMKQKLAIAVSLMNKPKFLILDEPTNGMDPDGSIDVLT
                     TIKSLVNELDMRILISSHKLEDIELICDRAVFLRDGHFVQDVNMEEGVASDTTIVTVD
                     HKDFDRTEKYLAEHFQLQNVDKADGHLMINAQKNYQVILKALSELDIYPKYIETRKSS
                     LRDTYFNINQRGDK"
BASE COUNT         3049 a         1429 c         1021 g         3139 t
ORIGIN      
        1 gcatattcaa ataaaaattc tatatatgct tcgatatgac atctatctag ttcatttaaa
       61 ctattccttg tttctttatt tttagatata ttttgaaaaa acctagtcaa aactcttata
      121 taaaatcttg ctgtagcaaa tgctatatca ccagttatca aacgattttt taaatatttt
      181 tttgctaatt ctcgcatttt aattgactca attttttcaa agttcaaata attaccagtt
      241 agtgttttat tataactcaa tccatatttt tctaaatttc taatatccca tttatctttt
      301 tcccattctt ctcgcttatc aataaattta tttagattaa tatacatatt ctttaaaaaa
      361 gaagctagtc cacttttatg ttcataatca ccaaaaacta ttgttgatga ttttcttttt
      421 ataggtatat tatttttagt caaccaacta aaccagtact tttctaacat attaatatca
      481 caatctagta aagaatttaa atttggataa acttcattaa agaactcagc aatcttgtta
      541 agtttcactg ctccagtatt aaatacatta gataaagacc aatcctcttt aactataagt
      601 ttcaaaaaaa ggtatttaaa ttctaaattc aaattttcac ttttaatcct aaagaaaata
      661 ttttttcttt tgtctttata gttcttgata ctatcttgaa attgctcgaa cttgttaaaa
      721 gctgaaatgt gccattcatc attttttaaa aagtaatcat catatatttc agttattagc
      781 acttctcctt ttatgtttat agttctactt tgtaatgtac ataattcatt aatcatagta
      841 ctgtttgtag gcaaattaat tggtattgac atagttattc ccctttatta aaaacttgaa
      901 attgatgctt aactttattc caatcttcac gtatatcttc ttcagtggga tgaacatata
      961 aattaatagt cgtttgtata ttactatgac cgagtctttc ttgaacttgt ttaatatcct
     1021 tagtttcatt ataaaaaaca gttgcatgtg tatggcgaaa taaatggggg tgaacattta
     1081 tagatgtttt atgttttagc cttttaaata atgaatatat atcactataa tgcatagctt
     1141 ctcctacatt tctacccttt atttttacaa aaagataatc agaacatatg gaaagttcgt
     1201 caattatttc gtacacataa tcgtcatata aatcaattaa cgattgagaa ataaatattt
     1261 ttctttcacc agtttttaaa taagtaccat tgctattaaa tctatttttc aaaactattt
     1321 ggtgaccttt tctaaaatca aatttgatat catcaatgcg taatgataat acctcaccta
     1381 tacgtaatcc tgtttcatat aataattgta ttaagaattt atctctaata ttattagctg
     1441 ctcctaataa tttcttaacc tcaacattgg ttaacacttc tagttttttc tttggttctt
     1501 ttactttcaa aatatttttc aacattccat agtccttgtt tacgtgatgc atgaaacttt
     1561 tatatttttt agagtattta ctttccgtat gtataaaatc atagaaatta acatctaatt
     1621 ttttgctcct atagagataa tcataaaaat tagatactac agtaatagtc agattaattg
     1681 tcttaggact aatgcttttt ccttttcggt gataagagag gacattctca tattcaaaag
     1741 gtgttttcat ccatcttata aaatcaacaa agttatcaaa actaacttct ttatagcata
     1801 tctttttact ttctaagtaa acaaaaaatt tttttagtgc ataggcatag gttttcttgg
     1861 tatttaaact tttattaaca ctatccagat acttcaaata tcttactgca tctactatag
     1921 gttcattatt accatctaaa atcataaaat tgataccatt cttagatttt acttctacta
     1981 ttttcataat aatctcattt ctaattttaa tagtataagt atacactatt cgaactcttt
     2041 ttacatcata catattaaac ttattacacg taataaaaaa agactattta atatttttac
     2101 tttgttcatt ttttcttaaa atatgattca ttttaaacaa aatagtctat atccttcaat
     2161 atatatataa ccttttgcaa cattacccga acaattcgaa acgattcaac aatacattat
     2221 cgaccaaaac aaaattacac gtcctgtatt atcagatgat caattggctg agctaaacat
     2281 tcggctacac gaggcgctac aatatgccca acctgtagaa gttaaatttt ataataatgg
     2341 ctttgtagat tcagtacgct taaccattta tcgtattgat gctattaatt atgaaattga
     2401 tggttatgtt tacaatcagc aacaacgtca aaaaatttct atctttgata tcttagatat
     2461 tgtcttgtta ccttaatgct ttgaacataa ttaaaggcaa gaacaatcta tattgtcctt
     2521 gcctaaatgt tattagttat ttttactttc gatatcagaa ataatttctt tagtttttct
     2581 ttcaatatct tcaaaatctt ttttaaagat ataagcaaat gctactacac caacaccagc
     2641 aactaatgtt gtaatagcaa ctaagctaaa tacgaattta aacacacatt taataaaatt
     2701 ccacatctca atttcacctc tgttaaatta aatacctatc tacatttcat tatactatca
     2761 tatatagaat aatcattaaa taaataccca cttttacgaa atatattcat caaattatat
     2821 agcgagcaag ctaatgatta ttttcaaaaa tgccgaggaa cctctgtttc aacgtgacat
     2881 gaccttatat gctaatgtta acaatgacta ttatcgtgaa tgctaactta agattgttat
     2941 ttatgataag atgattttaa gtttttaact taatatcccc accatctata aaaatatgtt
     3001 ttaactgatt gataagtctt atacaccgag aaatcacctc aaacaatttt atatttcatt
     3061 tagtaaaata taactttatt taacatgaca ttcaatatac atacgattac tatttattta
     3121 caatatatta caactaaaaa gaaaggggcc gatgatatgc aacaagtgac atcagatatt
     3181 atgactttta gaggttcaca tttcgagtta ggtgttaaaa ctggtaaatg gcttcaacaa
     3241 acacctcttc taaaaaatcg agaaaaagaa tggaaaaaac gtgtcccacg tttcgatata
     3301 gatgtaaatg aaacttatca aatattccaa acttacgcac ctcaaatatg ggaagaatta
     3361 atgggactac aaagtatact caaaatgcca acacgccaaa ttattttaaa ctttggacat
     3421 tatcgtttta ccgatttaaa agaaagtggt tgcacggtct ttcaaggcaa agacttcatg
     3481 gttagaaatt atgattacca ccctgcaact tatgatggtc gctacttact atatcaacct
     3541 acagatagcg gtttagctca aattggtcct gtatcaaggg taactggaag aatggatggt
     3601 atgaatgagt caggattaac tatgggctat aactttatgc accggaagaa accagcaaat
     3661 ggttttgtat gttatatgat tggtcgtcta attcttgaaa attgccgaaa tgtaacagaa
     3721 gcaatccaat tattgaaaga aataccacac agaagctcat ttagctacat actgatggac
     3781 aaatcattga accatgcaat tgttgaagtg acgccgagat caatagatgt tcgttatgat
     3841 aatatatgta caaatcattt tgaaatatta acacatgaaa atcgcaatta tacaaaagaa
     3901 tctaaagaaa gactggcgcg taccatttca caaactaatg ataatttaga tatgactaca
     3961 gcatttaagt tattcaataa tcctcagtat gaaatttaca gtaaactatt caaaagctgg
     4021 tctggcacca tccatacatc aatgtatcac cctgaaacat taactgcata ttttacattg
     4081 ggagaaaatg cgccaccaga aataatagat ttcaaatcat ggttagatgg tcaagagctt
     4141 aatatcactc actttactgg caatatagat accgacttaa cttttgccaa caaataaata
     4201 acaccgatat tagagaaatt atgcttccta atatcggtgc ttacatcctt tatttattca
     4261 actttgatat taccatcaat cgtataaaat tctaaaacat tgtcgctttt cccaacttta
     4321 ccattagtaa atgttttatt tttaactacg ctatcacccg ttcccggatt tagctttagt
     4381 atagtgtctt caggtttact atcatactta aaacgaatat ctccttgttt agtagaagct
     4441 tttgcatcta cttttgatgg catgttttta aattcaatgt caccatgatc gtttaaaaat
     4501 atggattgtt taatttgact cttgtcgatt tgtagcgtac caataacaac tttaatatta
     4561 ctatttttaa tgttactttt actaatataa gtagaactat tatttgtctt tgaatctagt
     4621 gcgtcaagat ttgagttttt aataactact tcaccgttaa tgctttgaat acttgtgttc
     4681 tctaaatcaa catcactgat tctaacactt cctgctccag acgatatatt taaacgttta
     4741 atcattttat caggcatttc aatcgttaaa gttttcttat tctcatgaaa aggattcata
     4801 tcaattgcat atcctcttgt cttagacctt ttatcactaa tcttcaacgt cttatctact
     4861 atattaatta atatatcatt gtcaccatca tatttaactc taaattgatt cccccgtttt
     4921 atacgaagtt ctgtactatc caaatttata gatacattgt caaaagcatc gtctttgaat
     4981 gttttatcat attgtttagt accatatttg tttttatcat agctgaacca aataatggtt
     5041 gctgctgtaa aaaagacaac aaacactaaa agcccaataa aaaagaattt cttcattttc
     5101 taacgcttcc tttgacagtt ttaatatacc aaagtaaata ttttaagatt aaacgataaa
     5161 tgtattctaa aatcttaaat atgacaatga taaatactaa tcctaatcct gaatatgata
     5221 tggcaaaaaa tacattacta attgagtcta caattcctct ggatattgct gatgctaata
     5281 aaataattgg acaaaacagc agcaaaagtg atattaaaaa taatgctaat acaaataatc
     5341 caactataga tactggtata aggatgacaa aaatagatag aatgcctaaa cttaatgaag
     5401 cagcaacagc tcttgaaata ttttcaaatg ttggtttaga ttcagcataa tcaataacac
     5461 ttctagcttt tgtttcagct gcaacatctt gtgggtcttt taacttcttt aaaatttcct
     5521 cttcatcaac gccattaagt tcttgctcat aaaaataacg ttcatattca tacatttttt
     5581 gatctcttaa atgcctaggt agcgcatcaa gtgctgtttc taattcattt aaataagtaa
     5641 tcttgttcat ataggtcact catcccaata aaattattca ttcgtttttt ataactttaa
     5701 gacctaaagc tatttactaa aaaattcaat acgtttaatt tctaaatctt gataaagtaa
     5761 gtttaatttt aaatggttat ctctatcaat atactttacc gatgagaaat cattgccaac
     5821 ttttgttctt ttatatccga acccaagatt ttcaatcgcg ttatttatcg gcgaaccaat
     5881 tcccccatca aaattagtat gtatcttttc atctcttact aatattatcc ccttaacgat
     5941 gttatccttt tctctcactt taacggtaag gtcagggtga gctttactgt tataaaattt
     6001 aaaacgatca aacttaattt ttttatttac ttcatagcct tttttatcaa acttctcatt
     6061 taatttaata tcgtttattt caagattaga caaatccaca gttttcgtat gacgatattg
     6121 atataaacca attaatacac caaaagaaca gattaaaaat aatataaaca caacccagcg
     6181 attttgaata cgcactgatg tcaccctttc tttgtactta tttaaatata gcataaggtg
     6241 cttcccgttt catccttaac aaaaattaac aaagaatttg caattaattt atttcattac
     6301 cttttattac tataaattgg tataatgttg taaatgctaa aggaggtttt tacaatgaca
     6361 aatttagaaa cttatttcaa aaatagccaa cccttaaatg aatacattga tggaatgaag
     6421 acaaatcaag ataccgttct cagtatctat caatcatttg agttaccaga agatgattca
     6481 cgcatcaaaa aaataaaaga gatgaattat tctaaagtat tagtaattac tgaagactgg
     6541 tgtggagatg caatgatgaa tctcccaatt ctaaaacaca tcagtgaagc attaaattta
     6601 gaagtacgcg tgttccatag agatgatgat acaaaactga tcgatcaata tttaacaaat
     6661 ggtaaatcac gtgcaattcc tatcttcgta ttcttgaatg atcaattcga acaagaaaca
     6721 gtttggggac caagagcaaa tgaagtccaa aaatttgtaa ctgatgttcg ttctgataaa
     6781 ttaccgagca aagatcatcc agactatgca gatttagaaa aagaaacaca cttagttata
     6841 tcaaaccgct ataaaactga cgcttcattt tggaaagcag tttataattc catcctaaat
     6901 aaattagaaa ataaataaat ttacaataag acaaactttg aggttgggaa aatttaaaat
     6961 tcccaacctc aaaattattt ttattaaatg tcttttttag cgacatataa gatattcaaa
     7021 atgaagaata tcactataga acctaatgaa attacccatt gccaattgct aaaatcaaag
     7081 ttagagctaa tcattttatc agttaagtaa gcaaaaggga tataatcaaa agcatctaaa
     7141 actttttcac cgtatcttgg gataaatgta ataaaaggtt taataaacgg tacaatgagt
     7201 actaaaaata cacctaatga aaatatcatt gctgtttttt gtgatgcaag tgtgattaag
     7261 aataaaaaca agccaaagaa tagtaaaaag attaggaaga accataaatt atctacatat
     7321 ttagaaaacg ccaaattttt tccattacta gatgcctgca caacaatcaa aatcacataa
     7381 tgaattactg caaataatat tgaaattaaa ataattgaaa ttgttttcga aataatgtat
     7441 ccaacgcgtg ttttaacttt actcatatat agttgaattg taccttgtga aaaatcacgc
     7501 gtaattgtct taacaacaaa cattaatcca attattaaca ataaccattt tgctgcagca
     7561 aatacagatc catagacaat aatatgtttc gggttatcca tcgaatttgc cataagtata
     7621 ctttgagttg caatcaaact agatacgact agtatcgcta aatatgttaa aggactttta
     7681 aatatactat aaaaatcata cttaactaaa tttaaaattc tcattattta tcacctcttt
     7741 gatttatatt gaagtacgta tcacgcaatg aacttttacg tgtttcaata tatttcggat
     7801 aaatatctaa ttcagataat gcttttagta taacttgata atttttttgt gcattgatca
     7861 ttaaatgtcc gtctgctttg tcgacatttt gtaattggaa atgctctgca agatattttt
     7921 cagttctatc aaagtcttta tgatcaacag taactatcgt tgtgtcagat gcaacacctt
     7981 cctccatgtt tacatcttga acaaaatgtc cgtctcttaa aaatacagct ctatcacaaa
     8041 ttaattcaat atcttctaac ttatgacttg atattagaat tctcatatca agttcattta
     8101 ctagagactt aattgtagtc agcacatcaa ttgagccatc tggatccata ccatttgtag
     8161 gctcatccaa gattaaaaat ttaggtttat tcattaaaga tactgcaatt gctaactttt
     8221 gcttcatccc cattgaatat ttcttaactt tctttttaat ataaggtctc ataccaaatg
     8281 catctataat tttgtctgtg tatgctttat caaaaccctt acctaatact tgtgcaaata
     8341 atttcaagtt atacaatcct gatttattat catataattt aggatgctca attaaaaaac
     8401 cgatattatc tgcattatca atgtctactt ttccatcaaa tttaataata ttaccattca
     8461 ttactttcat aacggttgtt ttaccaacac cgttttttcc tattaatccg acaattctac
     8521 tatcgccaaa gtcaaaatca atatcattta aaacgacatt tgagccgtat ttttttgtaa
     8581 tatgttctaa tttcatcgac tgcctccttt aaattttcta gtatttattt tattgagc
//
LOCUS       DAHHOJ010000107         8617 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.107, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000107 DAHHOJ010000000
VERSION     DAHHOJ010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8617)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8617)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8617
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.107"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(91..402)
                     /locus_tag="NBH32_002188"
     CDS             complement(91..402)
                     /locus_tag="NBH32_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1515766.1"
                     /translation="MTKLKYIIPTIIAVAIVIISTISIIQFINRKRYNPVKVLNEVKS
                     YFMNVKGSYIVYEPFVHPETDKYRLVYQGGITTIKNGQNIHYDFYADAYTGEVINIVE
                     R"
     gene            489..1565
                     /locus_tag="NBH32_002189"
     CDS             489..1565
                     /locus_tag="NBH32_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HCD1515767.1"
                     /translation="MNINKKVTLQRIQTLTELHGAPGFEEEVKNYMTQQMAPYVDEFI
                     ENRMGGFFGVKKSKNPNAKRVMIAAHMDEIGFMITNITKNGMIQFTNLGGVANDIWQG
                     QRLVIKNRNGDKIIGVVSNIPKHFRTGSEGAPEIKDLTLDIGAQNEDEVRERGIDIGD
                     TIVPHTPFTQLSEHRYSAKAWDNRYGCVLAIEILELLKDIELDVDLYVGANVQEEVGL
                     RGAKASAEMIDPDVAFVVDCSPANDVKGNQPLSGELGKGTLIRIKDGTMILKPVFRDY
                     LLKLVEAHDIEHQYYMSPGGTDGGEIHKANIGIPTAVIGVCARYIHSTDSVFDIRDYF
                     AARSLLSEAICNLDNNQIETLQYK"
     gene            1630..1941
                     /locus_tag="NBH32_002190"
     CDS             1630..1941
                     /locus_tag="NBH32_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1515768.1"
                     /translation="MKQLESEQQFESLKQGATVFEFTAGWCPDCRVIEPDLPELEARY
                     PMFDFVSVDRDKFMDICIENGIMGIPSFLVYKNGELLGSYIGKERKSIEQIDAFLAQY
                     V"
     gene            2041..2898
                     /locus_tag="NBH32_002191"
     CDS             2041..2898
                     /locus_tag="NBH32_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1444 domain-containing protein"
                     /protein_id="HCD1515769.1"
                     /translation="MNTFQMRDKLKERLSHLDVDFKFNREEETLRIYRTDNNKGITIK
                     LNAIVAKYEDKKEKIVDEIVYYVDEAIAQMADKTLESISSSQIMPVIRATSFDKKTKQ
                     GVPFIYDEHTAETAVYYAVDLGKSYRLIDESMLEDLKLTEQQIREMSLFNVRKLSNSY
                     TTDEVKGNIFYFINSNDGYDASRILNTAFLNEIEAQCQGEMLVAVPHQDVLIIADIRN
                     KTGYDVMAHLTMEFFTKGLVPITSLSFGYKQGHLEPIFILGKNNKQKRDPNVIQRLEA
                     NRRKFNKDK"
     gene            2927..3523
                     /locus_tag="NBH32_002192"
     CDS             2927..3523
                     /locus_tag="NBH32_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HCD1515770.1"
                     /translation="MNLFYNPKYVGDVAFLQIEPVEGELNYNKKGNVVEITNEGNVVG
                     YNIFEISKDITIEEKGHIKLTDELVNVFQKRISEAGFDYKLNADLSPKFVVGYVETKD
                     KHPDADKLSVLNVNVGNDTLQIVCGAPNVEAGQKVVVAKVGAVMPSGMVIKDAELRGV
                     ASSGMICSMKELNLPNAPEEKGIMVLNDSYEIGQAFFE"
     gene            3544..7368
                     /locus_tag="NBH32_002193"
     CDS             3544..7368
                     /locus_tag="NBH32_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCD1515771.1"
                     /translation="MSWFDKLFGEDNDSNDDLIHRKKKRRQESQNIDNDHDSLLPQNN
                     DIYSRPRGKFRFPMSVAYENENVEQSADTISDEKEQYHRDYRKQSHDSRSQKRHRRRR
                     NQTTEEQNYSEQRGNSKISQQSIKYKDHSHYHTNKPGTYVSAINGIEKETHKSKTHNM
                     YSNNTNHRAKDSTPDYHKESFKTSEVPSAIFGTMKPKKLENGRIPVSKPSEKVESDKQ
                     KYDKYVAKTQTSQNKQLEQEKQNDSVVKQGTASKSSDENVSSTTKSMPNYSKVDNTIK
                     IENIYASQIVEEIRRERERKVLQKRRFKKALQQKREEHKNEEQDAIQRAIDEMYAKQA
                     ERYVGDSSLNDDSDLIDNSTDASQLHTNGIENETVSNDENKQASIQNEDTNDTHVDES
                     PYNYEEVSLNQVSTTKQLSDDEVTVSNVTSQHQSALQHNVEVNDKDELKNQSRLIADS
                     EEDGATNKEEYSGSQIDDAEFYELNDTEADEDTTSNIEDNTNRNASEMHVDAPKTQEH
                     AVTESQVNNIDKTVDNEIELATRHKKDDQTNLSVNSLKTNDVNDGHVVEDSSMNEIEK
                     HNAEITENVQNEAAESEQNVEEKTIENVNPKKQTEKVSTLSKRPFNVVMTPSDKKRMM
                     DRKKHSKVNVSELKPVQSKQAVSESKPASQAAPSSSSDSQESNTNAYKTNNMTSNNVE
                     NNQLIGHAETENDYQNAQQYSEQKPSADSTQTEIFEESQDDNQLENEQVDQSTSSSVS
                     EVSDITEESEETTHPNNTSGQQDNDDQQKDLQPSFSNQNEDTANENRPRTNQQDVATN
                     QAVQTSKPMIRKGPNIKLPSVSLLEEPQVIEPDEDWITDKKKELNDALFYFNVPAEVQ
                     DVTEGPSVTRFELSVEKGVKVSRITALQDDIKMALAAKDIRIEAPIPGTSRVGIEVPN
                     QNPTTVNLRSIIESPSFKNAESKLTVAMGYRINNEPLLMDIAKTPHALIAGATGSGKS
                     VCINSILMSLLYKNHPEELRLLLIDPKMVELAPYNGLPHLVAPVITDVKAATQSLKWA
                     VEEMERRYKLFAHYHVRNITAFNKKAPYDERMPKIVIVIDELADLMMMAPQEVEQSIA
                     RIAQKARACGIHMLVATQRPSVNVITGLIKANIPTRIAFMVSSSVDSRTILDSGGAER
                     LLGYGDMLYLGSGMNKPIRVQGTFVSDDEIDDVVDFIKQQREPDYLFEEKELLKKTQT
                     QSQDELFDDVCAFMVNEGHISTSLIQRHFQIGYNRAARIIDQLEQLGYVSSANGSKPR
                     DVYVTEADLNKE"
     gene            7392..>8617
                     /locus_tag="NBH32_002194"
     CDS             7392..>8617
                     /locus_tag="NBH32_002194"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCD1515772.1"
                     /translation="MTHYHFVGIKGSGMSSLAQIMHDLGHEVQGSDIENYVFTEVALR
                     NKGIKILPFDANNIKEDMVVIQGNAFASSHEEIVRAHQLKIDVVSYNDFLGQIIDQYT
                     SVAVTGAHGKTSTTGLLSHVMNGDKKTSFLIGDGTGMGLPESDYFAFEACEYRRHFLS
                     YKPDYAIMTNIDFDHPDYFKDINDVFDAFQEMAHNVKKGIIAWGDDEHLRKIEADVPI
                     YYYGFKDSDDIYAQNIQITDKGTAFDVYVDGEFYDHFLSPQYGDHTVLNALAVIAISY
                     LEKLDVTNIKEALETFGGVKRRFNETTIANQVIVDDYAHHPREISATIETARKKYPHK
                     EVVAVFQPHTFSRTQAFLNEFAESLSKADRVFLCEIFGSIRENTGALTIQDLIDKIEG
                     ASLINEDSINVLEQFD"
BASE COUNT         3309 a         1196 c         1613 g         2499 t
ORIGIN      
        1 taaaaaaatg agccacatcc aatcttacta attagggtgt ggctcatttt taagttttac
       61 gatccaaatc aaatatggat aaaattcgta ttaacgctct acaatgttaa tgacttcacc
      121 agtatatgca tctgcataaa aatcataatg aatattttga ccatttttaa tagttgtaat
      181 tccaccttga taaactaaac ggtatttatc agtttcagga tgaacgaatg gttcataaac
      241 gatatatgag cctttgacat tcataaaata tgattttact tcattaagta ctttaacggg
      301 attatagcgt ttacgattaa taaattggat gattgaaatg gtagaaataa ttacaatggc
      361 tactgcaatt attgttggaa ttatatattt cagtttagtc atcatttaag ctcccttgat
      421 ttcaaattca tatcattagt ttaccatatt gaagatgata taataactta taaggagtga
      481 gaacttttat gaacataaat aaaaaagtaa cattacaacg aattcaaact ttaactgagc
      541 ttcatggggc accaggtttt gaagaagaag taaaaaatta tatgactcag caaatggcgc
      601 cgtacgtaga tgaatttatt gaaaatcgta tgggtggatt ttttggtgtg aaaaaatcta
      661 aaaatccaaa tgcaaaacgt gtaatgattg cagcacatat ggatgaaatc ggatttatga
      721 ttacaaatat cactaaaaat ggaatgattc aattcacaaa tttaggtggt gttgcaaatg
      781 atatttggca aggacaacgc ttagtaatta aaaatagaaa tggcgataaa attatcggtg
      841 ttgtttctaa tatacctaaa cattttcgta ctggtagtga aggtgcaccg gaaattaaag
      901 atttaacatt agatataggt gctcaaaatg aagatgaggt gcgtgagcgc ggaatagata
      961 taggagatac aattgtacct cacacgccat tcacacagtt atctgaacat cgatatagtg
     1021 ctaaagcatg ggataatcgt tatggttgtg tcttggcaat tgaaatacta gaattattaa
     1081 aagatataga attagatgta gacttgtatg ttggcgcaaa tgttcaagaa gaggttggat
     1141 tacgaggtgc gaaagcatct gcagagatga tagacccaga cgttgcattt gtagtcgatt
     1201 gttcacctgc caatgacgtt aaaggaaacc aaccattatc tggtgaactt ggtaaaggga
     1261 cgttaattcg cataaaagac ggtacaatga ttttaaagcc tgtatttaga gattatttat
     1321 taaagttagt agaagcacat gacattgaac atcaatacta tatgtcacca ggtggaacag
     1381 atggtggaga aattcataaa gctaatattg gtattccgac tgcagttatt ggtgtatgtg
     1441 cacgatatat tcatagtaca gactcagtat ttgatataag agactatttt gcagctagat
     1501 ctttactttc agaagccatt tgtaatttag ataataatca aatagaaaca ttacaatata
     1561 aataatcggg taataacaac tattatctct aaatagttat atataatcat taattaagga
     1621 gacataaaaa tgaaacaact tgaatcagaa caacaatttg aatctttaaa acaaggtgct
     1681 acagtgtttg aattcactgc aggctggtgt ccagattgta gagtgataga accagattta
     1741 ccggaattag aagcgagata tcctatgttt gacttcgtat cagtagaccg tgacaaattt
     1801 atggatattt gtattgaaaa tggtattatg gggattccaa gttttctagt atataaaaat
     1861 ggtgaactgc ttggaagtta tattggaaaa gaacgaaaat caattgaaca gatagatgca
     1921 tttttagctc aatacgtgta atttagacta gagaaaaacg gggtaatact cgtttttctc
     1981 tgttactatg tgttgattta ttgtaaacta ttataaggtg cgaaattagg agtgttacat
     2041 atgaatacct ttcaaatgag agataaatta aaggaacgtt taagccattt agacgttgat
     2101 tttaaattta atcgtgaaga agaaactttg cgtatttatc gaacagataa taacaaaggt
     2161 atcacgatta aacttaacgc tatagtcgca aaatatgaag ataaaaaaga aaaaattgta
     2221 gatgaaattg tttattacgt tgatgaagct attgcacaaa tggcagataa aacccttgag
     2281 agtatatcat ctagtcaaat tatgcctgtc attagagcga ctagctttga taaaaaaact
     2341 aaacaaggtg ttccttttat ctatgatgag catactgcag aaacagcagt ttattatgca
     2401 gtcgatttag ggaaatcata tcgtctaatt gacgaaagca tgttagagga tttgaagtta
     2461 actgaacaac aaataagaga aatgtctctg tttaatgtta gaaaattgtc aaattcatat
     2521 acgactgatg aagtaaaagg taatattttt tattttatta actcaaatga cgggtatgat
     2581 gcaagtagga tactaaatac tgcattttta aatgaaattg aggcacaatg tcaaggcgaa
     2641 atgctcgtag cagtgccaca ccaagatgtg ttaattattg cagatatacg caataaaaca
     2701 ggatatgatg tgatggcaca tttaacaatg gaatttttca ctaaaggtct agttccaatt
     2761 acatcattat cctttggata taaacagggt catcttgaac cgatatttat tttaggtaaa
     2821 aataataaac aaaaaagaga tccaaacgtg attcagcgtt tagaagcaaa tcgtcgtaaa
     2881 tttaataaag ataaatagaa ataattggat aaggagtttt gtcataatga atttatttta
     2941 caatcctaaa tatgtaggag atgtcgcatt tttacaaatt gaaccagttg aaggtgaatt
     3001 aaactacaat aaaaaaggta atgttgttga aattactaat gaaggtaatg ttgtaggtta
     3061 taatattttt gaaatttcaa aagatataac aattgaagaa aaaggtcata ttaaattaac
     3121 tgatgaactt gtaaatgtat tccaaaagcg tatttcagaa gctggttttg attataaatt
     3181 aaatgctgat ctatcaccga aatttgtagt tggctacgtt gaaactaaag acaaacatcc
     3241 tgatgcagat aaattaagtg tactaaatgt aaacgttgga aatgacacat tacaaattgt
     3301 atgtggcgcg cctaacgttg aagctggaca gaaagttgtt gttgctaaag taggtgcagt
     3361 gatgcctagc ggtatggtaa ttaaagatgc tgaattacgt ggtgttgcct caagcggtat
     3421 gatttgttca atgaaagaat tgaatttacc taatgcacct gaagaaaaag gtattatggt
     3481 attaaatgac agctatgaaa ttggacaagc attttttgaa taattaagga aggtagtgaa
     3541 aatatgagct ggtttgataa attattcggc gaagataatg attcaaatga tgacttgatt
     3601 catagaaaga aaaaaagacg tcaagaatca caaaatatag ataacgatca tgactcatta
     3661 ctgcctcaaa ataatgatat ttatagtcgt ccgaggggaa aattccgttt tcctatgagc
     3721 gtagcttatg aaaatgaaaa tgttgaacaa tctgcagata ctatttcaga tgaaaaagaa
     3781 caataccatc gagactatcg caaacaaagc cacgattctc gttcacaaaa acgacatcgc
     3841 cgtagaagaa atcaaacaac tgaagaacaa aattatagtg aacaacgtgg gaattctaaa
     3901 atatcacagc aaagtataaa atataaagat cattcacatt accatacgaa taagccaggt
     3961 acatatgttt ctgcaattaa tggtattgag aaggaaacgc acaagtcaaa aacacataat
     4021 atgtattcta ataatacaaa tcatcgtgct aaagattcaa ctccagatta tcacaaagaa
     4081 agtttcaaga cttcagaggt accatcagct atttttggca caatgaaacc taaaaagtta
     4141 gaaaatggtc gtatccctgt aagtaaacct tcagaaaaag ttgagtcaga taaacaaaaa
     4201 tatgataaat atgtagctaa gacgcaaacg tctcaaaata aacaattaga acaagaaaaa
     4261 caaaatgata gtgttgtcaa acaaggaact gcatctaaat catctgatga aaatgtatca
     4321 tcaacaacaa aatcaatgcc taattattca aaagttgata atactatcaa aattgaaaat
     4381 atttatgctt cacaaattgt tgaagaaatt agacgtgaac gagaacgtaa agtgcttcaa
     4441 aagcgtcgat ttaaaaaagc gttgcaacaa aagcgtgaag aacataaaaa cgaagagcaa
     4501 gatgcaatac aacgtgcaat tgatgaaatg tatgctaaac aagcggaacg ctatgttggt
     4561 gatagttcat taaatgatga tagtgactta atagataata gtacagatgc tagtcagctt
     4621 catacaaatg gcatagagaa tgaaactgta tcaaatgatg aaaataaaca agcgtcaata
     4681 caaaatgaag acactaatga cactcatgta gatgaaagtc catacaatta tgaggaagtt
     4741 agtttgaatc aagtatcgac aacaaaacaa ttgtcagatg atgaagttac ggtttcgaat
     4801 gtaacgtctc aacatcaatc agcactacaa cataacgttg aagtaaatga taaagatgaa
     4861 ctaaaaaatc aatccagatt aattgctgat tcagaagaag atggagcaac gaataaagaa
     4921 gaatattcag gaagtcaaat cgatgatgca gaattttatg aattaaatga tacagaagca
     4981 gatgaggata ctacttcaaa tatcgaagat aataccaata gaaacgcgtc tgaaatgcat
     5041 gtagacgctc ctaaaacgca agagcacgca gtaactgaat ctcaagttaa taatatcgat
     5101 aaaacggttg ataatgaaat tgaattagca acgcgtcata aaaaagatga ccaaacaaac
     5161 ttaagtgtca actcattgaa aacgaatgat gtgaatgatg gtcatgttgt ggaagattca
     5221 agcatgaatg aaatagaaaa gcataacgca gaaattacag aaaatgtgca aaacgaagca
     5281 gctgaaagtg aacaaaatgt cgaagagaaa actattgaaa acgtaaatcc aaagaaacag
     5341 actgaaaagg tttcaacttt aagtaaaaga ccatttaatg ttgtcatgac gccatctgat
     5401 aaaaagcgta tgatggatcg taaaaagcat tcaaaagtca atgtgtctga attaaagcct
     5461 gtacaaagta aacaagctgt gagtgaaagc aagcctgcga gtcaagccgc accatcatca
     5521 agctctgatt cacaagagtc aaacacaaat gcatataaaa caaataatat gacatcaaac
     5581 aatgttgaga acaatcaact tattggtcat gcagaaacag aaaatgatta tcaaaatgca
     5641 caacaatatt cagagcagaa accttctgct gattcaactc aaacggaaat atttgaagaa
     5701 agccaagatg ataatcaatt ggaaaatgag caagttgatc aatcaacttc gtcttcagtt
     5761 tcagaagtaa gcgacataac tgaagaaagc gaagaaacaa cacatccaaa caatactagt
     5821 ggacaacaag ataatgatga tcaacaaaaa gatttacagc cttcattttc aaatcaaaat
     5881 gaagatacag ctaatgaaaa tagacctcgg acgaaccaac aagatgttgc aacaaatcaa
     5941 gctgtacaaa catctaagcc gatgattcgt aaaggcccaa atattaaatt gccaagtgtt
     6001 tcattactag aagaaccaca agttattgag ccggacgagg actggattac agataaaaag
     6061 aaagaactga atgacgcatt attttacttt aatgtacctg cagaagtaca agatgtaact
     6121 gaaggtccaa gtgttacaag atttgaatta tcagttgaaa aaggtgttaa agtttcaaga
     6181 attacggcat tacaagatga cattaaaatg gcattggcag cgaaagatat tcgtatagaa
     6241 gcgcctattc caggaactag tcgtgttggt attgaagttc cgaaccaaaa tccaacgacg
     6301 gtcaacttac gttctattat tgaatctcca agttttaaaa atgctgaatc taaattaaca
     6361 gttgcgatgg ggtatagaat taataatgaa ccattactta tggatattgc taaaacgcca
     6421 cacgcactaa ttgcaggtgc aactggatca gggaaatcag tttgtatcaa tagtattttg
     6481 atgtctttac tatataaaaa tcaccctgag gaattaagat tattacttat cgatccaaaa
     6541 atggttgaat tagctcctta taatggtttg ccacatttag ttgcaccggt aattacagat
     6601 gtcaaagcag ctacacagag tttaaaatgg gccgtagaag aaatggaacg acgttataag
     6661 ttatttgcac attaccatgt acgcaatata acagcattta acaaaaaagc accatatgat
     6721 gaaagaatgc caaaaattgt cattgtaatt gatgagttgg ctgatttaat gatgatggct
     6781 ccgcaagaag ttgagcagtc tattgctaga attgctcaaa aagcgagagc atgtggtatt
     6841 catatgttag tagctacgca aagaccatct gtcaatgtaa ttacaggttt aattaaagcc
     6901 aacataccaa caagaattgc atttatggta tcatcaagtg tagattcgag aacaatatta
     6961 gacagtggtg gagcagaacg cttgttagga tatggcgata tgttatatct tggtagcggt
     7021 atgaataaac cgattagagt tcaaggtaca tttgtttctg atgacgaaat tgatgatgtt
     7081 gttgatttta tcaaacaaca aagagaaccg gactatctat ttgaagaaaa agaattgttg
     7141 aaaaaaacac aaacacaatc acaagatgaa ttatttgatg atgtttgtgc atttatggtt
     7201 aatgaaggac atatttcaac atcattaatc caaagacatt tccaaattgg ctataataga
     7261 gcagcaagaa ttatcgatca attagagcaa ctcggttatg tttcgagtgc taatggttca
     7321 aaaccaaggg atgtttatgt tacggaagca gatttaaata aagaataatt atgagtaagg
     7381 agttttatat aatgacacac tatcattttg tcggaattaa aggttctggc atgagttcat
     7441 tagcacaaat catgcatgat ttaggacatg aagttcaagg atcggatatt gagaactacg
     7501 tatttacaga agttgctctt agaaataagg ggataaaaat attaccattt gatgctaata
     7561 acataaaaga agatatggta gttatacaag gtaatgcatt cgcgagtagc catgaagaaa
     7621 tagtacgtgc acatcaattg aaaatagatg ttgtaagtta taatgatttt ttaggacaga
     7681 ttattgatca atatacttca gtagctgtaa ctggtgcaca tggtaaaact tctacaacag
     7741 gtttattatc acatgttatg aatggtgata aaaagacttc atttttaatt ggtgatggca
     7801 caggtatggg attgcctgaa agtgattatt tcgcttttga ggcatgtgaa tatagacgtc
     7861 actttttaag ttataaacct gattacgcaa ttatgacaaa tattgatttc gatcatcctg
     7921 attattttaa agatattaat gatgtttttg atgcattcca agaaatggca cataatgtta
     7981 aaaaaggtat tattgcttgg ggtgatgatg aacatttacg taaaattgaa gcagatgttc
     8041 caatttatta ctatggattt aaagattcgg atgacattta tgctcaaaat attcaaatta
     8101 cggataaagg tactgctttt gatgtgtatg tggatggtga gttttatgat cacttcctgt
     8161 ctccacaata tggtgaccat acagttttga atgcattagc tgtaattgcg attagttatt
     8221 tagagaagct agatgttaca aatattaaag aagcattaga aacgtttggt ggtgttaaac
     8281 gtcgtttcaa tgaaactaca attgcaaatc aagttattgt agatgattat gcacaccatc
     8341 caagagaaat tagtgctaca attgaaacgg cacgaaaaaa atatccacat aaagaagttg
     8401 ttgcagtatt tcaaccacac actttctcta gaacacaagc atttttaaat gaatttgcag
     8461 aaagtttaag taaagcagat cgtgtattct tatgtgaaat ttttggatca attagagaaa
     8521 atactggtgc attaacgata caagatttaa ttgataaaat tgaaggtgca tcgttaatta
     8581 atgaagattc tattaatgta ttagaacaat ttgataa
//
LOCUS       DAHHOJ010000108         8589 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.108, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000108 DAHHOJ010000000
VERSION     DAHHOJ010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8589)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8589)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8589
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.108"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(268..1146)
                     /locus_tag="NBH32_002195"
     CDS             complement(268..1146)
                     /locus_tag="NBH32_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exonuclease"
                     /protein_id="HCD1515773.1"
                     /translation="MPNKILLVDGMALLFRHFYATSLHKQFMYNSQGVPTNGIQGFVR
                     HIFSAIHEIRPTHVAVCWDMGQSTFRNDMFDGYKQNRSAPPEELIPQFDYVKEISEQF
                     GFVNIGVKNYEADDVIGTLAQQYSTDNDVYIITGDKDLLQCINDNVEVWLIKKGFNIY
                     NRYTLHRFNEEYDLEPQQLIDIKAFMGDTADGYAGVKGIGEKTAIKLIQQYQSVENVV
                     ENIDALSAGQRNKINDNLDELYLSKRLAEIHTQVPIDSEALFEKMSFATTLNHILSIC
                     NEHELHVSGKYISSHF"
     gene            complement(1166..4606)
                     /locus_tag="NBH32_002196"
     CDS             complement(1166..4606)
                     /locus_tag="NBH32_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HCD1515774.1"
                     /translation="MINKEQLDLLYKLKKEVEKSRNEALLHTINQVIKKVYLQQYTCS
                     FVGHFSAGKSTLINLLIEQDILPSSPVPTTSNTAIVSVSDNHDIIANLPNQTYAKLSN
                     YDEVREMNRQNVDVESVEINFQSTKFENGFTLQDTPGVDSNVASHQSITEQYMYTSNM
                     IFYTVDYNHVQSELNFKFMKHINDVGIPVVFIINQIDKHQDDELSFSTFKSRVEKSIA
                     DWGIKLERIFYVSKFDHPENELEVLSTYLVSLDQHRETIEDYTLRTVEYITEAQLDYI
                     QSEIQEVLEDLGIEEAEFEQAFLNSQQHQAISEEAQLLNNPDELMAFLKNKRKNILEN
                     AYIMPHNMREMLRSYLESMSQDFNVGGFFNKKKKKLQIQQQRLLTATDALQEHVNQQI
                     RQPMREDMSFVTRFINKKEASDKVLNQHYDVKSEMIEALYQPQTSISNTYVLTFSDEV
                     VKAIKKYVEQQSTPIFKEIIENVQADELPTEESDDLKEYQRYTELNELRQSLTTKNYR
                     HYYIHLDESLDKLVDRQETTYQVATDNAQGNRDNQQLNQNTATTNMSIDIQKALDIIS
                     DVPLFKRTKQDIHETLTRIDNKLIKIGVFGTFSAGKSSLINALLGEHILVSSPNPTTA
                     ATTEISYGDESYITLKSQSQLLDEINAVVEYQDISFSTIEDFINSDLEKLKSHLNKNQ
                     LAFVHAVEKHYKLYVSMLENGEKHAINQQELKKWSAEDEYATFVKTVHIALMHDWLKG
                     KIIVDSLGLHSNNQRHTNETEQILTSSDLILYVSYFNHSFTDNDKAFIEHMKDMNQLN
                     ENQAFKMVINAADLAESQDDLEAVETYVSDALRQVHLQSDIFAVSSRNALQAEDKGID
                     QLKQSIQQFVDVDSKSILEQQMIHQLQQMDRSYVEMITEFETNKADISRRQQRLTDYK
                     DKQRLQHQLIDATLQHTDNEVEEQVYHLNARLKLQLLDDVKSVFNSQMTQNSDFNEEK
                     KVSTKVYLDQIHQRLFLEQSLITERIKKYFNKQLTEQIAPIVQQLADLHVIVNPQFNF
                     ESANIEQPLLHIDFNDMLNALPKQLTKRKILNPNGQRDIHESICQSTLGLLQPQMGLL
                     RQQLELYVKQMAVEAESQFESFEANIQTQINDLLAFDLDTTLINQLKDKHQQLKTILY
                     "
     gene            4910..6250
                     /locus_tag="NBH32_002197"
     CDS             4910..6250
                     /locus_tag="NBH32_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HCD1515775.1"
                     /translation="MKNTFNPLQRLHFYAAIFIAPLLITLTISGIGYLFFPEVENNIY
                     KNKFFGDSDVKTHQTLNDAVHQVEQQYEGFFVSKVSILDEPYNKRITLSDMAGNQRYV
                     FLDRNNQFVADQNAKHTYSNVMRSIHSSLFTENTIINYLVELTACWTIFMILSGTYLL
                     IKKHLISNKSKALRWQKWHAMIGVIIAIPVFVLVITGLPWSGFMGSKIAGMMDTNGDL
                     GQGELAINPPKSDLNELPWATRKNKQPASSEKGSSGHHGNAAMPQTKLDYQISIDKVV
                     EQAQKSGIKKPFSIVYPSDRNGAFIVSNTSNSGVTGLDVSPYKEQTLYFDQYSGKKLG
                     TIKYDDYGIIAKWFTWGIPLHEGHLFGILNKIINLFVCIALLVAIGMGFVSWIKRTKN
                     TAVKVPHRVKKPASISLIICLIVLGLLMPLFGLSLILVFIIELILYIKDRRAKQ"
     gene            complement(6459..6791)
                     /locus_tag="NBH32_002198"
     CDS             complement(6459..6791)
                     /locus_tag="NBH32_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD1515776.1"
                     /translation="MGKKMGLGLSIALVVIGIAVVCLMIFSSQKTTYFGYMNSNTNAE
                     KVVSEKDGLVKHNIKVEPSNDFKPKKGDFVKLVSKDGGKTFYKQEIVKHDDVPHGLMM
                     KIHDMHMN"
     gene            complement(6875..8020)
                     /locus_tag="NBH32_002199"
     CDS             complement(6875..8020)
                     /locus_tag="NBH32_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HCD1515777.1"
                     /translation="MFQLLAVCPMGLEAVVAREIQELGYETNVENGRIFFEGDASAIV
                     KANLWLRTADRIKIVVGRFNATTFDELFEQTKALPWESIIDKEGNFPVQGRSVKSTLH
                     SVPDCQAITKKAIVERLRRAYNEKGWLNESGAKYPVEVAILKDNVLLTIDTSGSGLNR
                     RGYRLAQGEAPIKETLAASLIRLANWKGDTPLIDPFCGSGTIAIEACLIAQNIAPGFN
                     REFVSEQWNIMPANIYDDYRDEADKMADYDKEIEVYASDIDPEMVEIAKRNAEEVGLS
                     DIIKFSVKDVNTLTIDTEEPVALIGNPPYGERIGDREEVEEMYRYIGKLMKQHPFLST
                     YILTSNKEFEYLVDRKATKRRKLFNGYIECTYYQYWGKKTERKTIEN"
     gene            complement(8229..>8589)
                     /gene="rnpB"
                     /locus_tag="NBH32_002200"
     ncRNA           complement(8229..>8589)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="NBH32_002200"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
BASE COUNT         2669 a         1485 c         1249 g         3186 t
ORIGIN      
        1 atacattgac tcaaaaacta aataattaaa tctctttttt aaatgaatga agttgtaact
       61 gtactatttt gtagtgcatg gtaatttatt tggatgaaat ataattcttt aactctattg
      121 attaatcaaa tatgtatcta tcaaaattcg acttatttat tcactttgac atatccaaat
      181 acgcaaaaag actatttcta ccttgtatcg atagaaatag tctttttata cttaattggt
      241 ttaatttttc ataaaatctt atacttttta aaaatgagat gaaatatatt ttcctgaaac
      301 atgtaattca tgttcgttgc aaattgataa aatatgatta agcgttgttg caaatgacat
      361 tttctcaaaa agcgcttcac tatcaattgg tacttgtgtg tgaatttcag ccagtcgctt
      421 cgataaatat agttcatcta aattgtcatt aatcttatta cgttgtccag ctgataatgc
      481 atcaatgttt tcaaccacat tttcaacgct ttgatattgc tgaattaact taatagccgt
      541 tttctcacca attcctttaa cacctgcata tccatctgct gtatcaccca taaacgcttt
      601 aatatcgatt agttgttgtg gttcaaggtc atattcttcg ttaaaacgat gtaatgtata
      661 tctattataa atgttaaaac cttttttaat tagccaaact tcaacattgt cattaataca
      721 ttgcagtaaa tctttgtcgc ccgtaataat atagacatcg ttatcagttg aatattgttg
      781 tgctaatgta cctataacat catccgcttc atagttttta acgccaatat ttacaaagcc
      841 aaattgctct gaaatttctt taacataatc aaattgtggt atcaattctt ctggtggtgc
      901 agaacgattt tgcttataac catcaaacat atcatttcta aaagttgatt gtcccatatc
      961 ccaacataca gctacatgtg tagggcgtat ttcatgtatt gccgaaaaga tatgacgcac
     1021 aaatccttgt attccatttg taggtacacc ttgtgaattg tacataaatt gtttatgaag
     1081 acttgtagcg tagaaatgtc taaataatag cgccatacca tctacaagta atattttatt
     1141 tggcatttaa aacgttcctt ctttcttaat ataaaatagt tttcagttgt tgatgtttat
     1201 ctttcaattg attgataagt gttgtatcta aatcaaatgc taataaatcg tttatttgcg
     1261 tttgaatatt agcttcaaaa ctttcaaatt gcgattcagc ttctacagcc atttgcttta
     1321 catataattc aagctgttgc ctcaataatc ccatttgtgg ttgtaataat cctaacgtac
     1381 tttgacaaat tgattcatgt atatctcttt gcccatttgg attcaaaatt ttacgttttg
     1441 ttaattgttt aggcaatgca tttagcatat cattgaaatc gatgtgcaat aatggttgct
     1501 ctatattagc tgattcaaag ttaaactgag gattaacaat gacatgtaaa tctgctaatt
     1561 gttgaacgat tggggcaatt tgctcagtga gttgcttatt aaagtatttt tttatacgtt
     1621 ctgtaattaa agattgctct aaaaacaatc gttgatgaat ttgatctaag tatactttcg
     1681 tagacacttt cttttcttca ttaaaatcac tattttgcgt catttgagaa ttaaacactg
     1741 atttaacatc gtcaagtagt tgtaatttta aacgtgcatt taaatgataa acttgttctt
     1801 caacttcgtt gtctgtatgt tgtagcgtcg catcaattaa ttgatgttgt aaacgctgtt
     1861 tatctttata gtctgttaac ctttgttggc gacgtgaaat atcagcttta tttgtttcga
     1921 attctgtaat catctctaca taagaacgat ccatttgctg aagctgatga atcatttgtt
     1981 gttctaaaat tgatttagaa tcaacatcaa caaattgttg tatgctttgt tttaattgat
     2041 caatgccctt atcttcagct tgcaatgcat ttcgacttga tacagcaaaa atgtctgatt
     2101 gtaagtgtac ttgtcttaat gcatctgata catatgtttc aaccgcttca agatcatctt
     2161 gactttctgc taaatcagca gcattaatta ccattttaaa tgcttggttt tcattcaact
     2221 ggttcatatc tttcatgtgt tctataaacg ccttgtcatt atcagtaaat gaatgattaa
     2281 aataactcac atacaatatt aagtctgaag aagttaaaat ttgctctgtt tcatttgtat
     2341 gcctttggtt atttgagtgt agccctaagg aatcaacaat tattttacct tttaaccaat
     2401 catgcattaa tgcaatgtgt actgttttaa caaatgttgc atattcatct tctgcactcc
     2461 actttttcaa ttcttgttga ttaatggcat gtttttctcc gttttccaac atactgacat
     2521 acaatttata atgtttttca actgcatgaa caaaagcgag ttgattttta tttaaatgtg
     2581 attttaactt ttctaaatct gaattaataa agtcttctat agttgaaaaa gatatatctt
     2641 ggtattcaac tactgcatta atttcatcta ataattgcga ttgtgatttt agcgttatat
     2701 aactctcgtc tccataagat atttctgtag tagctgctgt agtaggattt ggagaactga
     2761 ctaaaatatg ctcgcctaat aatgcattta tcaaactact tttacccgca ctaaatgttc
     2821 caaatacgcc tatttttatt aatttattat ctatacgtgt taatgtttcg tggatatcct
     2881 gctttgtacg cttgaacaaa ggcacatccg aaattatatc aagtgctttt tgaatatcta
     2941 tagacatatt tgttgtagcg gtattctgat ttagctgttg attatctcga ttaccctgag
     3001 cattatcagt agccacttga tatgttgtct cttgtcgatc tactaattta tctagagatt
     3061 catctaaatg aatatagtag tgacgataat tcttagtcgt caatgactga cgcagctcat
     3121 ttaattctgt ataacgttga tattctttta aatcatcact ttcttctgtt ggtaattcat
     3181 ctgcctgcac attttctata atttctttaa aaatcggtgt tgactgttgt tcaacgtatt
     3241 tcttaatggc tttaaccact tcgtctgaaa atgtaagtac ataagtattg ctgattgatg
     3301 tttgtggttg atataaagct tcaatcattt ccgacttaac gtcataatgc tgatttaata
     3361 ctttatctga agcttctttt ttattgataa aacgtgtaac aaatgacata tcttctcgca
     3421 ttggttgacg aatttgttga ttaacatgtt cttgtaacgc atctgtcgct gttaataatc
     3481 gctgttgttg aatttgtagc tttttcttct ttttattaaa aaatccgcca acattaaagt
     3541 cttgagacat actttccaaa taacttcgta acatttctct catattatgc ggcataatgt
     3601 atgcattttc taaaatattt ttacgcttat tctttaaaaa tgccattaat tcatctggat
     3661 tatttaaaag ttgtgcctct tcactaattg cttgatgctg ttgactattt aaaaatgctt
     3721 gttcaaactc tgcttcttcg atacccaaat cttctaatac ttcctgaatt tcagactgaa
     3781 tataatctag ctgagcttcg gtaatatatt caaccgttct taatgtataa tcctctattg
     3841 tttctctatg ttgatctaat gaaactagat aagttgataa tacttcaagt tcattttcag
     3901 ggtgatcaaa tttagataca taaaagatgc gttctaattt aatgccccaa tcagcaattg
     3961 atttttcaac tcgagattta aacgtagaga atgacaattc atcgtcttga tgcttgtcaa
     4021 tttgattaat gataaacaca acaggtattc caacatcatt tatatgcttc ataaacttaa
     4081 agttaagttc agattgaacg tggttatagt caaccgtata aaatatcata ttgcttgtat
     4141 acatatattg ttctgttatt gactgatgtg atgcaacatt tgaatcaaca cctggtgtat
     4201 cttgcaacgt aaacccattt tcaaatttag ttgattgaaa attaatttct acagattcaa
     4261 cgtcgacatt ttggcgattc atttccctta cttcatcata attagataat ttggcatacg
     4321 tttgattcgg caaattagca ataatatcgt gattgtctga aactgacacg atagcagtat
     4381 tacttgtcgt tggtacaggt gaacttggta agatatcttg ttcaattaat aaatttatca
     4441 gtgtcgattt acctgcagaa aaatgtccaa cgaacgaaca tgtatattgc tgcaaatata
     4501 ctttcttaat tacttggtta attgtatgta aaagtgcttc atttcgcgac ttttcaactt
     4561 ctttttttaa tttatataaa agatctaatt gttctttatt aatcatgtta ctttcccctt
     4621 tggttcgttc acatagaaat gcttgcttgt taaaacgaat tcatacaata tcgatatcca
     4681 tttaaacaaa gatcgttgaa tggctcgcat tgtattatgt ttaaatttta tcatagctgc
     4741 ttgataaaac atacgaataa tcaattttct tcaaacattc caacttaaaa gttcattttt
     4801 ctaaatatca caaatcatcg ttaaattttt ctagaaaata aatattgtca cagtttgttc
     4861 acacacgtca cagaattgac accgtataat agccaattgg gagggaaata tgaaaaatac
     4921 atttaatcca ttacaaagat tacatttcta tgcagcaata tttattgctc cactgttaat
     4981 cactttaacc atttcgggca ttggttactt gttctttcca gaagttgaaa ataatattta
     5041 taagaataag ttttttggtg acagtgatgt aaaaacgcat caaacattaa atgatgcagt
     5101 acatcaagtt gaacaacaat atgaaggatt ctttgtaagt aaagttagca tacttgatga
     5161 accatataac aaacgaatta cactgagtga tatggcagga aatcaacgtt acgtctttct
     5221 agatcgtaac aatcaatttg ttgcagatca aaatgcgaaa catacgtatt ctaatgtgat
     5281 gcgaagtata catagttctt tgtttactga aaatactatt attaattatt tagtagagtt
     5341 aaccgcatgt tggacgatat tcatgatttt atctggtact tatttactca ttaagaagca
     5401 tttaatttct aacaaaagta aggcacttcg ttggcaaaag tggcacgcaa tgattggagt
     5461 tatcattgca attccagtat ttgtattagt cataactgga ttgccatggt ctggttttat
     5521 gggtagtaaa attgccggta tgatggacac aaacggtgac cttggtcaag gtgaattagc
     5581 gattaatcca cctaaatcag atttgaacga attaccttgg gctacacgta aaaataaaca
     5641 gccagcttca tccgaaaaag gttcaagtgg tcatcatggt aatgcagcaa tgcctcaaac
     5701 caaattagat tatcaaatat ctattgataa ggtcgttgaa caggcgcaaa aatctggtat
     5761 taaaaagccg ttttcaatcg tatatccaag tgatagaaat ggtgccttta ttgtatctaa
     5821 tactagtaat tcaggtgtta ctgggctaga tgtatcacct tacaaggaac aaacacttta
     5881 tttcgatcaa tatagcggta aaaagttagg tacgattaaa tatgatgact acggtattat
     5941 tgctaaatgg tttacatggg gcattccgct tcacgaaggt catttattcg gcattttaaa
     6001 taaaatcatt aatttatttg tatgtatcgc tttattagta gccattggca tggggtttgt
     6061 ctcttggata aagcgtacaa aaaatactgc agtaaaagta ccacatcgcg taaaaaaacc
     6121 agcatctata tcactcataa tatgtttaat tgtattagga ttattaatgc cattatttgg
     6181 attatcactt atccttgtat ttataattga attaatatta tatattaaag atcgtcgtgc
     6241 taaacaataa tgcacttaaa gttttgaact gacgaaattt acaaaatgat tctcgacact
     6301 ataattactt aaaacgggtt ctataataaa tcgtactgat gaaaaagttt tttacttttc
     6361 catcagtacg attttttgaa tttgaagata aaaaagcatc taaaacgcat aagaagataa
     6421 ctctacttct tccaaattac gttatagatg cttttttatt aattcatatg catgtcgtga
     6481 attttcatca ttaaaccgtg tgggacgtca tcatgtttaa caatctcttg tttataaaat
     6541 gtcttcccac catctttaga aactaatttt acaaagtctc cttttttcgg cttgaaatca
     6601 ttagatggtt ctactttgat attatgtttg actaatccat ctttttcact gacaactttt
     6661 tctgcatttg tattactatt catataacca aaataagtcg ttttttgact agaaaaaatc
     6721 attaaacata caacggcaat accaataaca accaatgcta tagataaacc tagacccatt
     6781 tttttaccca tttatagact cctccatttt tcaaaaatac ctattatcat gagaacataa
     6841 ttattactaa ttttagtcac gcattataaa cactttagtt ttcgatagtt ttacgttctg
     6901 tctttttacc ccagtattga taataagtac attctatata tccattaaat aacttacgtc
     6961 gcttcgttgc tttacgatct actaaatatt caaattcttt attacttgtt aaaatgtatg
     7021 tagataaaaa tggatgttgt ttcattagtt taccaatata acggtacatt tcttcaactt
     7081 cttcacgatc accaatacgt tcaccatatg gaggatttcc aattaacgcc accggttctt
     7141 ctgtatcaat tgttaatgta ttgacatctt ttacactaaa tttaataata tcagacaacc
     7201 caacttcttc agcattacgc ttagcaatct ctaccatttc tggatcaata tcagaagcat
     7261 atacttcgat ttccttatca taatcagcca tcttatccgc ttcatcacgg taatcatcat
     7321 aaatatttgc tggcatgatg ttccattgct ctgatacgaa ttcgcgatta aaaccaggtg
     7381 cgatattttg agcaattaaa caagcttcta tagctatagt acctgaaccg caaaatggat
     7441 caattaaagg tgtatcacct ttccagtttg cgagacggat taaacttgct gccaacgttt
     7501 ctttaattgg tgcttcacct tgtgctaatc tatatccacg tctgttcaaa ccagaacctg
     7561 atgtgtcgat ggtcaataat acattatctt ttaaaatggc aacttcaaca gggtatttgg
     7621 cacctgattc atttaaccaa cccttttcgt tatatgcacg acgtaatcgt tcaacaatag
     7681 ctttcttagt tatcgcctga caatctggca cactatgtag tgttgattta acgcttctac
     7741 cttgaactgg gaagttacct tctttatcaa taatagattc ccaagggagc gctttggttt
     7801 gttcgaataa ttcgtcaaac gttgttgcgt taaaacgtcc aacaacaatt ttgattcggt
     7861 ctgctgtgcg caaccataaa tttgccttta caattgcact tgcgtctcct tcaaaaaata
     7921 tacgaccatt ttcaacattt gtttcatagc ctaattcttg aatttcccta gcaacaacag
     7981 cttctaatcc catcggacaa actgcaagta attgaaacat atatgattct ccttttatac
     8041 aggtatttta ttcttagctt gtgtttttta tacatttcca acaaatttaa tcgctgatac
     8101 attaacgcat tcgcttatta ttttaaaaca aggcagtgtc attatatcaa gacaaggcgt
     8161 taattttaag tgttcttttc atgaaaaaag ctctccatca tctaggagag ctaaactagt
     8221 agtgatattt ctataagcca tgttctgttc catcgtactc atcacgtgca ctagtcacac
     8281 tggtactcag gtgataacca tctgtctaca ccacttcatt tcgcgaagtg tgtctcgttt
     8341 atacgttgaa ttccgttaaa caagtgctcc taccaaattt ggattgctca ctcgaggggt
     8401 ttaccgcgtt ccacctttta tatttctata aaagctacgt cactgtggca ctttcaaatt
     8461 actctatcca tatcgaaaga cttaggatat ttcattgccg tcaaattaat gccttgattt
     8521 attgtttcat caagcacgaa cactacaatc atctcagact gtgtgagcat ggactttcct
     8581 ctatataat
//
LOCUS       DAHHOJ010000109         8338 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.109, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000109 DAHHOJ010000000
VERSION     DAHHOJ010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8338)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8338)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8338
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.109"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            70..1248
                     /gene="deoB"
                     /locus_tag="NBH32_002201"
     CDS             70..1248
                     /gene="deoB"
                     /locus_tag="NBH32_002201"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCD1515778.1"
                     /translation="MTRPFNRVHLIVMDSVGIGEAPDAADFKDEGSHTLRHTLEGFDQ
                     TLPNLEKLGLGNIDKLPVVNAVEQPEAYYTKLSEASVGKDTMTGHWEIMGLNIMQPFK
                     VYPNGFPEELIQQIEEMTGRKVVANKPASGTQIIDEWGEHQMKTGDLIVYTSADPVLQ
                     IAAHEDIIPLEELYDICEKVRELTKDPKYLIGRIIARPYVGEPGNFTRTSNRHDYALK
                     PFGKTVLDHLKDGGYDVIAIGKINDIYDGEGVTEAVRTKSNMDGMDQLMKIVKKDFTG
                     ISFLNLVDFDALYGHRRDKPGYAQAIKDFDDRLPELFSNLKEDDLVIITADHGNDPTA
                     PGTDHTREYIPVIMYSPKFKGGHALESDTTFSSIGATIADNFNVTLPEFGKSYLKELK
                     "
     gene            complement(1379..2194)
                     /gene="phnE"
                     /locus_tag="NBH32_002202"
     CDS             complement(1379..2194)
                     /gene="phnE"
                     /locus_tag="NBH32_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD1515779.1"
                     /translation="MTQEIAKYNVHTKAHKRKLIKRWLIAIVVLAIIIWAFAGVPSLE
                     LKSKSLEILKSIFSGLFHPDISYIYIPDGEDLLRGLLETFAIAVVGTFIAAIICIPLA
                     FLGANNMVKLRPVSGVSKFILSVIRVFPEIVMALIFIKAVGPGSFSGVLALGIHSVGM
                     LGKLLAEDIEGLDFSAVESLKASGANKIKTLVFAVIPQIMPAFLSLILYRFELNLRSA
                     SILGLIGAGGIGTPLIFAIQTRSWDRVGIILIGLVLMVAIVDLISGSIRKRIV"
     gene            complement(2191..2991)
                     /gene="phnE"
                     /locus_tag="NBH32_002203"
     CDS             complement(2191..2991)
                     /gene="phnE"
                     /locus_tag="NBH32_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HCD1515780.1"
                     /translation="MPLEIPTKYDSLLKKKVSLKTSFTFMLIIVLIIWSFIYTGFNFG
                     DLMIGIPQIGDLFKQMIPPDFEYLQQITTPMLDTIRMAIVSTVLGSIVSIPIALLCAS
                     NIVHQKWISIPSRFILNIVRTIPDLLLAAIFVAVFGIGQIPGILALFILTICIIGKLL
                     YESLETIDPGPMEAMTAVGANKIKWIVFGVVPQAISSFMSYVLYAFEVNIRASAVLGL
                     VGAGGIGLFYDQTLGLFQYPKTATIILFTLVIVVVIDYISTKVRAHLA"
     gene            complement(2993..3766)
                     /gene="phnC"
                     /locus_tag="NBH32_002204"
     CDS             complement(2993..3766)
                     /gene="phnC"
                     /locus_tag="NBH32_002204"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCD1515781.1"
                     /translation="MSQIEFKNVSKVYPNGHVGLKNINLNIEKGEFAVIVGLSGAGKS
                     TLLRSVNRLHDITSGEIFIQGKSITKAHGKALLEMRRNIGMIFQHFNLVKRSSVLRNV
                     LSGRVGYHPTWKMVLGLFPKEDKIKAMDALERVNILDKYNQRSDELSGGQQQRISIAR
                     ALCQESEIILADEPVASLDPLTTKQVMDDLRKINQELGITILINLHFVDLAKEYGTRI
                     IGLRDGEVVYDGPASEATDDVFSEIYGRTIKEDEKLGVN"
     gene            complement(3980..4936)
                     /locus_tag="NBH32_002205"
     CDS             complement(3980..4936)
                     /locus_tag="NBH32_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1515782.1"
                     /translation="MKNFKCLFVLMLAVIVFAAACGNSSSLDNQKNASNDSDSKSGGY
                     KPKELTVQFVPSQNAGTLEAKAKPLEKLLSKELGIPVKVSVSTNYNTIVEAMKSKKVD
                     VGFLPPTAYTLAHDQKAADLLLQAQRFGVKEDGSASKELVDSYKSEILVKKDSKIKSL
                     KDLKGKKIALQDVTSTAGYTFPLAMLKNEAGINATKDMKIVNVKGHDQAVISLLNGDV
                     DAAAVFNDARNTVKKDQPNVFKDTRILKLTQAIPNDTISVRPDMDKDFQEKLKKAFID
                     IAKSKEGHKIISEVYSHEGYTETKDSNFDIVREYEKLVKDMK"
     gene            5165..6709
                     /locus_tag="NBH32_002206"
     CDS             5165..6709
                     /locus_tag="NBH32_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCD1515783.1"
                     /translation="MKKIYKSLTVSAIVATISLSVLPQSLAITHESQHTKQQRTVLFD
                     RSHGQTAGAADWVSDGAFSDYADSIQKQGYDVKAIDGHSNITEASLKSSKIFVIPEAN
                     IPFKESEQAAIVNYVKHGGNVVFISDHYNADRNLNRIDSSEAMNGYRRGAYEDMSKGM
                     NAEEKSSTAMQGVKSSDWLSTNFGVRFRYNALGDLNTSNIVSSKESFGITEGVKSVSM
                     HAGSTLAITNPEKAKGIVYTPEQLPAKSKWSHAVDQGIYNGGGKAEGPYVAISKVGKG
                     KAAFIGDSSLVEDSSPKYVREDNGEKKKTYDGFKEQDNGKLLNNITVWMSKDNDGKSL
                     KASGLTLDTKTKLLDFERPERSTEPEKEPWSQPPSGYKWYDPTTFKVGSYGSEKGADP
                     QPNTPDDHTPPNQNEKVSFDIPQNVSVNEPFEVTIHLKGFEANQTLENLRVGIYKEGG
                     RQIGQFSSKDNDYNPPGYSTLPTVKADENGNATIKVNAKVLESMEGSNIRLKLGDKTL
                     ITTDFK"
     gene            6760..8295
                     /locus_tag="NBH32_002207"
     CDS             6760..8295
                     /locus_tag="NBH32_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HCD1515784.1"
                     /translation="MSNIAFYVVSDVHGYIFPTDFTSRNQYQPMGLLLANHVIEQDRR
                     QYDQSFKIDNGDFLQGSPFCNYLIAHSGSSQPLVDFYNRMAFDFGTLGNHEFNYGLPY
                     LKDTLRRLNYPVLCANIYENDSTLTDNGVQYFQVGDQTVGVIGLTTQFIPHWEQPEHI
                     QSLTFHSAFETLQQHLPEMKRHADIIVVCYHGGFEKDLESGTPTEVLTGENEGYAMLE
                     AFSKDIDIFITGHQHRQIAERFKQTAVIQPGTRGTTVGKVVLSTDEYENLSVESCELL
                     PVIDDSTFTIDEDDQHLRKQLEDWLDYEITTLPYDMTINHAFEARVAPHPFTNFMNYA
                     LLEKSDADVACTALFDSASGFKQVVTMRDVINNYPFPNTFKVLAVSGAKLKEAIERSA
                     EYFDVKNDKVSVSADFLEPKPQHFNYDIYGGVSYTINVGRPKGQRVSNMMIQGHAVDL
                     KQTYTICVNNYRAVGGGQYDMYIDAPVVKDIQVEGAQLLIDFLSNNNLMRIPQVVDFK
                     VEK"
BASE COUNT         2788 a         1382 c         1534 g         2634 t
ORIGIN      
        1 gttcaaatta tgcaaggttt agaagcagat tcagattact aatatatata aaatttggga
       61 gtgatagcta tgacaagacc atttaatcgt gtacatttaa tcgtaatgga ttcagtaggt
      121 attggtgaag cgccagacgc agctgatttt aaagatgaag gttcacatac tttaagacat
      181 accttagaag gcttcgatca aactttacca aaccttgaaa agttaggtct agggaacatc
      241 gataaattac cagtagtaaa tgcagttgaa caaccagaag catactatac taaattgagt
      301 gaagcttcag ttggtaaaga tacaatgact ggtcactggg aaattatggg attaaatatt
      361 atgcagcctt ttaaagtata ccctaatgga ttccctgaag agttaattca acaaattgaa
      421 gaaatgacag gtcgtaaagt tgttgctaac aaaccggcat cgggtacgca aattatcgat
      481 gagtggggcg agcaccaaat gaaaactggt gacttaattg tttatacaag tgcagaccca
      541 gtattgcaaa ttgctgcaca tgaagacatt atcccattag aagagttata tgatatttgt
      601 gaaaaggttc gtgagttgac aaaagaccct aaatatttaa ttggtcgtat tatcgcacgt
      661 ccatatgttg gtgaaccagg aaactttaca cgtacatcta atcgacatga ctatgcgtta
      721 aaaccttttg gtaaaactgt cttagatcat ttgaaagacg gtggttatga tgttattgcc
      781 atcggtaaaa ttaatgacat ttatgatggt gaaggtgtaa cagaagcggt tcgtacgaag
      841 agtaacatgg acggtatgga tcaattgatg aaaattgtta agaaagattt cacaggtatt
      901 agcttcttaa acttagtaga ctttgatgca ttatacggtc atcgtcgtga taaaccaggt
      961 tatgcacaag caattaaaga tttcgatgat cgcttgccag aactgtttag caacttaaaa
     1021 gaagacgatt tagtaattat tacagcagac catggtaatg acccgacagc gccaggtacg
     1081 gaccatacga gagaatatat cccagtaatt atgtacagtc cgaaatttaa aggtggtcat
     1141 gcactagaaa gtgatactac attcagttct atcggtgcaa ctatagcaga taatttcaac
     1201 gtaacattac cagagttcgg taaaagttat ttaaaggaat tgaaatagaa taaatttaga
     1261 tattataaaa acagcagtga agttaactat aacaatagtt ttcttcactg ctgtttttat
     1321 tataatagag aaacgtaaga cggtaggacc tcttatttag gagtatcctg atttaatgtt
     1381 aaacaatacg ttttcggatt gaaccggaaa ttaaatcgac aattgcgacc attagtacta
     1441 aaccgattaa tataatacct acacggtccc aagaacgtgt ttgaatggca aatatgagtg
     1501 gtgtcccgat accaccagcc ccaattagcc ccagtataga agctgaacgt aagtttagtt
     1561 caaagcgata aagtatgagt gatagaaagg caggcataat ttgtggtatg actgcaaata
     1621 cgagtgtttt aatcttattc gcaccactgg cctttaatga ttctacagca ctgaaatcta
     1681 gaccttcaat atcttcagct aaaagtttcc caagcatacc tacagaatgg atacctaaag
     1741 ctaatacacc tgaaaatgaa cctgggccaa cagctttgat aaatataagt gccattacaa
     1801 tttctgggaa gacacgtata acacttaaaa taaatttgct aacacctgaa accgggcgta
     1861 actttaccat attatttgca cctaaaaatg ctaatggaat acagataatt gcggcgatga
     1921 aagtacctac aacggctatc gcaaaggttt caagtaaacc acgtaataag tcttcgccat
     1981 ctggtatata gatatagcta atatctggat ggaataatcc gctgaatatg gattttaaga
     2041 tttctaatga tttactttta agttctaaac ttggtacacc tgcaaatgcc cagatgataa
     2101 tagctaagac gacaattgca ataagccatc ttttaatcaa ttttcgtttg tgtgcttttg
     2161 tgtgaacatt atattttgct atttcctgtg tcatgcgaga tgtgccctca ctttcgtact
     2221 gatgtaatca atgacgacga cgataactaa agtaaataaa ataatcgttg ctgtttttgg
     2281 atattgaaat aaaccaagtg tttgatcata aaacaatcca ataccgccag cgccgactaa
     2341 tccaagcaca gctgaagcac gtatatttac ttcaaatgca tataatacgt atgacataaa
     2401 tgacgatatg gcttgtggta caacaccgaa aacaatccat tttattttat tagcgccaac
     2461 agccgtcatt gcttccattg gacctggatc tatcgtttcc aatgattcat ataataattt
     2521 tccaataata cagatagtta aaataaacaa tgctaatatc cctggaattt gaccgattcc
     2581 aaatacagcc acaaagattg ctgctaataa caaatctgga atagtacgaa ctatatttaa
     2641 aataaagcgc gagggtattg aaatccactt ttgatgaacg atattgctag cacataataa
     2701 cgcaattggt attgaaacga tgctacctaa tactgtactt acgatagcca ttcgaatggt
     2761 atctaacatt ggcgttgtaa tttgttgtaa atactcgaaa tcaggtggaa tcatttgttt
     2821 gaatagatca cctatttgag gtattcctat cattaaatct ccaaaattaa accccgtata
     2881 aatgaagctc caaatgataa gcacaatgat taacatgaag gtaaaactcg tttttaaaga
     2941 aacctttttc tttaaaaggg agtcatactt tgtaggtatt tctaaaggca tgttagttca
     3001 ctcctagctt ttcatcttct ttaattgtac gtccatatat ttcactaaat acgtcatctg
     3061 ttgcttcaga tgcaggacca tcatagacaa cttcaccatc acgtaaacca atgatgcgtg
     3121 tgccatattc ttttgccaag tcaacaaaat gtaaattaat taaaattgtg atgcctaatt
     3181 cttggttgat ttttcttaaa tcatccataa cctgtttcgt tgttaatggg tctaatgaag
     3241 caactggttc atctgcaaga ataatttcgg attcttggca tagcgcacgt gcaatagata
     3301 tacgttgttg ttggccacct gataattcat cagagcgttg attatattta tctaagatat
     3361 tgacgcgttc tagtgcatcc attgctttaa ttttgtcttc ttttgggaat aaacctaata
     3421 ccattttcca agtagggtga taacctacac gtccacttag tacatttcgt aatacacttg
     3481 accgtttaac taaattaaaa tgttggaaaa tcatacctat atttcggcgc atttctaata
     3541 atgctttacc atgggcttta gtgatggatt tgccttggat gaaaatttca cctgacgtga
     3601 tatcatgcaa acgatttaca gatcttaata acgtggattt cccagcacca gatagtccga
     3661 caataactgc aaattcacct ttttcaatat ttaagttaat atttttcaag cctacatgac
     3721 cgttaggata gactttactg acgtttttaa attcgatttg actcatgttt gacacctttc
     3781 tttaataaag aaaagccaag gtaaactgaa atatcataga cattgggttt ctgtgtacaa
     3841 atggtttcaa atgtacagcg acacctttct atatctgttt cagtttagcg cctagctttt
     3901 caatattaat ctagaatata tctattgaac gaaagctttt aataccaaat tcgctaatga
     3961 ttcatttgtt aaataatgat tatttcatat ctttaactaa tttttcgtac tctcttacaa
     4021 tgtcgaaatt tgaatctttc gtttctgtgt atccttcatg tgaataaact tcgctaataa
     4081 ttttgtgacc ttcttttgat ttagcaatgt ctataaaagc ttttttcaat ttttcttgaa
     4141 aatctttatc catatctggt cttacagaaa ttgtgtcatt cggaatagct tgtgttaatt
     4201 ttaaaattcg tgtgtcttta aatacatttg gttggtcttt tttcacagta ttacgtgcat
     4261 cattaaatac agccgcagca tctacatctc catttaataa tgagataact gcttggtcat
     4321 gacctttaac attcacaatt ttcatatctt tagttgcatt aatacctgct tcgtttttta
     4381 acatcgcaag tgggaatgta tatccagcag ttgatgttac atcttgtaag gcaattttct
     4441 taccttttaa atctttcaag cttttaattt ttgagtcttt tttaacaaga atttctgatt
     4501 tataactatc tacaagttct ttacttgctg aaccatcttc ttttacacca aaacgttgtg
     4561 cttgtaataa taaatcagct gctttttgat catgtgctaa tgtgtatgcc gttggtggta
     4621 agaaaccaac atcaactttt ttagacttca tagcttcaac aattgtattg tagttagttg
     4681 atacagacac tttaactgga attcctaatt ctttagatag taatttttct aatggttttg
     4741 ctttagcctc taatgttcca gcattttgcg aaggtacaaa ttgtactgtt aattctttag
     4801 gtttgtatcc tcctgattta gaatccgaat cattactagc gttcttttga ttatctaaag
     4861 aacttgagtt tccacatgct gctgcaaaaa caatgactgc taacattaat acaaataaac
     4921 acttaaaatt tttcatttga taactgtccc cttcacattc ttaatcgtgt aatatgtatg
     4981 caatgaaatt acacttatag cgtaccacat ttaaatgcga aaaagtattt tataaaaatg
     5041 aattttaata actttcttta agagtgtgat aagattttgt aaacttttcg ttaaattgct
     5101 catatgcaac gaatttttat cttataattc aagaacaaat ttattttaaa aggagcctca
     5161 aaaaatgaaa aaaatatata agtcattaac tgtctctgca attgttgcaa cgatatcatt
     5221 aagtgtgcta ccgcaatctt tagctataac gcatgaatcg caacatacaa agcaacagcg
     5281 aacggtatta ttcgatcgtt ctcatggtca aacagctggt gctgcagatt gggttagtga
     5341 tggtgcattt tcagattatg cggattcaat acaaaaacaa ggttatgacg ttaaagctat
     5401 tgatggtcat tcgaacataa cagaagcaag tttgaaaagt tccaaaatat ttgtaattcc
     5461 tgaggctaac attcctttca aagaatcaga acaggcagca attgttaact atgtgaaaca
     5521 tggtggcaat gttgtcttta tttcagatca ttacaatgct gaccgaaatt taaatcgtat
     5581 tgattcatca gaggcaatga atggttatcg acgtggagcg tatgaagata tgtcgaaagg
     5641 tatgaatgca gaagaaaaaa gttctactgc aatgcaaggt gtgaaaagtt cagattggtt
     5701 atctacaaac tttggcgtac gttttcgata taatgcacta ggtgatttaa atacgagcaa
     5761 tattgtttct tcaaaagaaa gtttcggtat tactgaaggt gtgaaatctg tatctatgca
     5821 tgccggatcg acattagcaa ttactaatcc agagaaagca aaaggtattg tgtatacacc
     5881 agaacaattg ccagcgaaaa gtaaatggtc acatgctgta gatcaaggta tttataatgg
     5941 gggcggtaaa gcagaaggtc cctatgtagc aatttctaaa gttggaaaag gtaaagcagc
     6001 atttatcggt gattcatcac ttgtggaaga tagttcgccc aaatatgtaa gagaagataa
     6061 tggagaaaag aagaaaacat atgatggttt taaagaacaa gacaacggta agctgttaaa
     6121 taatataacg gtttggatgt ctaaagataa tgatgggaaa tcacttaagg cgagtggcct
     6181 aacattagat acaaagacta agttgcttga ttttgaacga ccagagcgtt caactgagcc
     6241 tgaaaaagag ccatggtcac aaccgccgag tggttataaa tggtatgatc caacaacatt
     6301 taaagtaggt agttatggca gcgaaaaagg cgcagatcct cagccaaaca caccagatga
     6361 tcatacgcca ccaaatcaga acgaaaaagt atcatttgat atcccgcaaa atgtttctgt
     6421 aaatgagcca tttgaagtga caatacattt aaaaggattt gaagcaaatc aaacacttga
     6481 aaatcttaga gttggtattt acaaagaagg cggacgtcaa atcggacaat tttcaagtaa
     6541 agataacgat tataacccgc caggttacag tactttgcca acagttaaag cagatgaaaa
     6601 cggaaatgcc acaattaagg tcaatgccaa agtactcgaa agtatggaag gttcaaatat
     6661 tcgtttaaaa ctcggtgaca aaaccttgat tacaacagac ttcaaataaa tatataatag
     6721 aaataagaaa gatgtttgtg attgaaggag tgagtgagga tgtcaaacat agcattttat
     6781 gtcgtgagtg acgtacacgg ttatattttc ccaacagatt ttacgagtag aaatcaatat
     6841 caacctatgg gattgttact agcgaatcat gttatagaac aagacagaag gcagtatgac
     6901 caaagtttta aaatagataa tggtgatttt ttgcaagggt caccattttg taattactta
     6961 atcgcgcata gcggcagtag ccagccttta gttgattttt ataatcgaat ggcattcgac
     7021 tttggtacgc ttggtaatca tgaatttaat tatggattac catacttaaa agacacttta
     7081 cgcagactca attatccagt tttgtgcgct aatatttatg aaaatgatag tacattgact
     7141 gataacggtg tgcagtattt tcaggttgga gatcaaacgg ttggtgtgat aggtttaacg
     7201 acacaattta ttccccattg ggaacaacca gagcatattc agtcacttac gtttcatagt
     7261 gcttttgaaa cacttcaaca acacttgcct gaaatgaagc gacatgcaga tatcattgtg
     7321 gtttgttacc atggtggatt tgaaaaggat ttagaaagtg gtacgccgac cgaagtgtta
     7381 acgggtgaaa atgaaggata tgccatgtta gaagcgtttt ctaaagatat agatatcttt
     7441 attacgggtc accaacatcg acaaattgct gaaaggttta agcaaacggc tgtgattcaa
     7501 cctggtacga gaggaacaac tgtaggcaaa gtagtcttga gtactgatga atatgaaaat
     7561 ctatccgttg aatcatgtga attacttcct gttatagatg attccacatt tactattgat
     7621 gaagatgacc aacatttacg aaagcagtta gaggactggt tagattacga aattactaca
     7681 ttgccatatg atatgacgat taatcatgca tttgaggcac gtgtggcacc gcatcctttt
     7741 acaaatttta tgaattatgc tttattagaa aaaagtgacg cagatgttgc ctgtacagct
     7801 ttgtttgatt ctgctagtgg tttcaagcaa gtcgtgacga tgcgagatgt gattaacaat
     7861 tacccatttc caaatacatt taaagtttta gctgtaagtg gtgccaaact taaagaagcc
     7921 attgaacgat cagcagaata ttttgacgtg aaaaatgata aagtaagtgt gagcgcagac
     7981 ttccttgaac ccaaaccaca acactttaat tatgatatat atggtggcgt aagttatacc
     8041 attaatgttg gcagaccaaa gggacaacgt gtgagtaata tgatgattca aggtcacgca
     8101 gttgatttaa agcagacata tacaatttgt gtaaataatt atcgtgcagt tggcggtggt
     8161 cagtatgata tgtatatcga cgcgccagtt gtaaaagata ttcaagttga aggcgcacaa
     8221 ttacttattg attttttatc aaataataat ttgatgcgca tcccgcaagt tgttgatttt
     8281 aaagttgaaa agtgacggat atatgtaaaa cttacttgat tttaatcggg taagtttt
//
LOCUS       DAHHOJ010000110         8239 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.110, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000110 DAHHOJ010000000
VERSION     DAHHOJ010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 8239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8239
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.110"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            116..596
                     /locus_tag="NBH32_002208"
                     /pseudo
     CDS             116..596
                     /locus_tag="NBH32_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(807..1607)
                     /locus_tag="NBH32_002209"
     CDS             complement(807..1607)
                     /locus_tag="NBH32_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1515785.1"
                     /translation="MVKAIAVDMDGTFLDSKKTYDKPRFEAIFTELRNRDITFIAASG
                     NQYAKLKSIFGDRDMYFISENGAVIYKGNELYNYKSFNRQVFQQVVDYLNMNQGIDQL
                     VICGLKSAYILKHTSEAFKEDTRFYYHQLKEIDSLQQLPEDDYVKIAFNINRETHPNV
                     DEEVATQFSNDIKLVSSGHDSIDIIMPNMTKGQALKRLLDKWEMSPSELMAFGDANND
                     KDMLAFAKHSYVMENSHDEELFNIASAVAPSNDKQGVLTIIEQEVLNK"
     gene            1928..2563
                     /locus_tag="NBH32_002210"
     CDS             1928..2563
                     /locus_tag="NBH32_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1515786.1"
                     /translation="MKLNNYSLKVKNKQLVDNCDLNFYLGQINHIVGKNGVGKSLLAK
                     DFLLNNSGNIPKSISQNVTLISSSSNIPNDITKDFLLSLLKSKFENNRQTFDKIYNIL
                     NIEAIPSNVLLKNLSDGQKQKLKLLSFLLEDHDLIILDEVTNALDKKTVNEIYEFLND
                     FIQSHQTKTIINITHNLSDLSALPGKYFIFKDLQIEEYQSKEEVINDYINL"
     gene            2569..3300
                     /locus_tag="NBH32_002211"
     CDS             2569..3300
                     /locus_tag="NBH32_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HCD1515787.1"
                     /translation="MSTLKLEFKKSISNKIIYTLGVLFIFLFLLGYFLPIGIDKVKSL
                     SYSQFFFSSYTVATQLGFLLFSFVIAYFINKEYSNKNILFYKLIGDNIFTFFYKKVAV
                     LFFECLVFIILSITIISIIYSDFSHYLLLIILFSLVILQYILVVGTISMVSPNILISL
                     GISIVYWIGSVILVAINKNIFGIVAPFEASNTMYRAVEKILNNESTFICPTEIINTVS
                     FFVLLFIVNTIVLLLSRKRWLKIGM"
     gene            complement(3462..4082)
                     /gene="srtA"
                     /locus_tag="NBH32_002212"
     CDS             complement(3462..4082)
                     /gene="srtA"
                     /locus_tag="NBH32_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase SrtA"
                     /protein_id="HCD1515788.1"
                     /translation="MKKWTNRLMTIAGVVLILVAAYLFAKPHIDNYLHDKDKDEKIEQ
                     YDKNVKEQASKDKKQQAKPQIPKDKSKVAGYIEIPDADIKEPVYPGPATPEQLNRGVS
                     FAEENESLDDQNISIAGHTFIDRPNYQFTNLKAAKKGSMVYFKVGNETRKYKMTSIRD
                     VKPTDVEVLDEQKGKDKQLTLITCDDYNEKTGVWETRKIFVATEVK"
     gene            4276..4767
                     /locus_tag="NBH32_002213"
     CDS             4276..4767
                     /locus_tag="NBH32_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCD1515789.1"
                     /translation="MIRCAKKEDLNAILVIYNDAIINTTAVYTYEPQTIDERVAWFET
                     KQRKHEPIFVFEENGSVLGFATFGSFRPWPAYLYTIEHSIYVDASARGKGIASQLLHH
                     LIVEAKAKGYRALVAGIDASNKASIQLHQKFAFKHAGTLTNVGFKFNQWLDLAFYELD
                     LQD"
     gene            complement(4916..5086)
                     /locus_tag="NBH32_002214"
     CDS             complement(4916..5086)
                     /locus_tag="NBH32_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515790.1"
                     /translation="MLRLSIIFIAFIINTTITYGYTTEGTWVNLLFKSLSLSMIIVFM
                     FYYIRFVIEKKR"
     gene            complement(5148..5246)
                     /locus_tag="NBH32_002215"
     CDS             complement(5148..5246)
                     /locus_tag="NBH32_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515791.1"
                     /translation="MIKKKHLSGSLVPDRCFLIISEEVEKFMGNTC"
     gene            complement(5265..6164)
                     /gene="sdaAA"
                     /locus_tag="NBH32_002216"
     CDS             complement(5265..6164)
                     /gene="sdaAA"
                     /locus_tag="NBH32_002216"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HCD1515792.1"
                     /translation="MFDSIRETIDYAVENNISFADIMVKEEMELSGKSRDEVRAQMKQ
                     NLDVMRDAVIKGTTGDGVESVTGYTGHDAAKLRDYNETHHALSGYEMIDAVKGAIATN
                     EVNAAMGIICATPTAGSSGTIPGALFKLEKTHDLTEEQMIDFLFTSALFGRVVANNAS
                     VAGATGGCQAEVGSASAMAAAAAVAIFGGSPEASGHAMALAISNLLGLVCDPVAGLVE
                     IPCVMRNAIGSGNALISADLALAGIESRIPVDEVIEAMDKVGRNLPASLRETGLGGLA
                     GTPTGEAIKRKIFGTAEDMVKNN"
     gene            complement(6178..6858)
                     /gene="sdaAB"
                     /locus_tag="NBH32_002217"
     CDS             complement(6178..6858)
                     /gene="sdaAB"
                     /locus_tag="NBH32_002217"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HCD1515793.1"
                     /translation="MAKSYDYQSAFDIIGPVMMGPSSSHTAGAVKIGNSARAILGDIP
                     KNIEIHYYESFAQTHQGHGTDVAIVGGAMGYSTFDNRIKSSLEIAADDGIKIDIIEEE
                     GDSIGQHPNCAYIKSSRNDGRYIEIIGISIGGGTIKIKGIHINGLEVDLNHGLPILVV
                     DGNMTKAQVNHFINDINDMKLDCKDEFIKIDEDKCLVVIPLNKAISESTLNTIKEKYS
                     DVNVSYIN"
     gene            complement(6861..7904)
                     /locus_tag="NBH32_002218"
     CDS             complement(6861..7904)
                     /locus_tag="NBH32_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCD1515794.1"
                     /translation="MDILLGVGTLVLVLVIMTLFLKYAPYGKQGLQALSGAACATFLP
                     QAFLSYAIGGVFDIKFLQDIGDLAGSLSGIAVGILTCLNLGVAPVFAVIVGLVLHDFK
                     LLPAFIAAYGVAFLIKWIEKKVPEGLDLIVVILFAPAIAFGLASIITPGVLATLKQIG
                     SAVTAVGDNNPYALAVILGLVIPVTGMTPLSSMVLTSLLGLTGVPMAIGALTCTGSSF
                     VNLILFRKLNIGGPSKAFAVCIEPLTQIDLIAQYPVQLYGTNALIGVVNACIVTFSGL
                     VIGVKGMATPIAGAIVLFGFNNAVTSIVTIATVIIVSIVLAYIIGTLINKFNLMNINF
                     KMPSKKNHIKESV"
BASE COUNT         2642 a         1505 c         1170 g         2922 t
ORIGIN      
        1 aagacacttt gccaaacttg cacataaatg tttaattcaa taatttgaat tttctgtgtt
       61 gggtcccttc gtataattta ataaatacca ctaaactaaa ttaatgaagt gccttatgta
      121 taaaaattat aacttgatcc aacttacact accaatagaa acttctgtta gaattcctca
      181 aattgatatt tcgcgatatg ttaatgaaat tgttgaaacg atacctaata gtgaattcga
      241 tgaattcaga caccatcgtg gcgcaacatc ttatcatcca aaaatgatgt aaaaattatc
      301 ttatatgcat acactcaatc tgtattttct ggtcgtagaa tagagaaact acttcatgac
      361 agtattcgaa tgatgtggtt agctcaaaat caaacacctt cttataaaac tattaatcgt
      421 tttagagtaa atcctaatac tgatgcatta attgaatctt tatttatcca gtttcatagt
      481 caatgtttaa agcaaaacct tattgatgat aattcgattt ttattgatgt acaaaagtag
      541 aagctaatgc caataggtat acatttgcgt ggaagaaaag tattcaaaat catgaattgt
      601 tttattttgt atatctgtat caaaatcgat ttgattaaaa tccgctttat tttatcatct
      661 attcaaatga ttttagtgcg cttattttac tatggcatta ttgtctaacc tgtttgagat
      721 atattaatat ttgatgattc gtattcagag ttttcattta acatgtattt agtatcataa
      781 tagctacttc attgatgatg tactacctac ttgttcaata cttcttgttc gataattgtt
      841 aaaacacctt gcttatcgtt acttggagcg acagcagagg caatattgaa caattcttca
      901 tcatgactat tttccatcac ataactatgc ttagcgaacg ctagcatatc tttatcatta
      961 ttcgcatctc caaaggccat gagctctgaa ggagacattt cccatttatc taacaatcgt
     1021 tttaatgcct gccctttagt catgtttggc ataataatat caatactatc gtgcccactt
     1081 gagacaagtt taatatcatt gctgaattgc gttgctactt cttcgtcaac attcggatgc
     1141 gtctcacgat taatattaaa tgctattttg acataatcat cctcaggtaa ttgttgtaga
     1201 ctatcaattt cttttaactg atgataataa aatctcgtat cttctttaaa tgcttcagaa
     1261 gtatgtttca aaatatacgc gcttttcaaa ccacagatga cgagttgatc aataccttgg
     1321 ttcatattta agtaatcgac aacctgttga aacacctgac gattaaagct tttataatta
     1381 tataactcat tgcctttata aataactgca ccattttcag aaataaaata catatctcta
     1441 tccccgaaaa tagacttcaa cttcgcatat tgattgccac tcgcagcaat aaatgtaata
     1501 tctctatttc taagttcagt aaaaatcgct tcaaatcttg gtttatcgta tgtctttttc
     1561 gaatctaaaa acgtcccgtc catatctacc gcaatagcct taaccatgat tatcctcctc
     1621 attgctcttt tagtcgtctt cattatatcc atctcatgac aagcaatgaa gtttgtcata
     1681 ccacaatgaa cctatattca cattttgaaa ttgctttcat atatgcaaaa cacattacac
     1741 tggatatagc attcgaaggc gaacataaat ttcagaatat tgtaaaaata taacgatata
     1801 tttatcattt ggacaaaata tagtgtgata tactaattta taacatctat acataatgtg
     1861 aaagaatttt caaaaataca attaaatatc ggtgtaataa atccctttat acataggagt
     1921 tgtatacatg aaactaaata actattcttt aaaagttaaa aacaaacaac tagttgacaa
     1981 ttgtgattta aatttctatc ttggtcagat caatcacatt gttggtaaaa atggtgtagg
     2041 aaaatcttta ttagctaaag atttcttact aaataatagt ggaaatatcc ctaagtccat
     2101 ttctcaaaat gtaaccttaa tatcaagttc atcaaatatt cctaatgata taacaaaaga
     2161 ttttttatta tcattgttaa aatcaaaatt tgaaaacaat cgacaaacat tcgataagat
     2221 ttataacata ctaaacatcg aagcaatacc gtctaacgta ctactaaaaa acttgagtga
     2281 tggtcaaaaa caaaagctta aattattaag tttcttattg gaagatcatg atttaatcat
     2341 attagatgaa gttacaaacg ctttagacaa gaaaacagtt aatgaaattt atgaattttt
     2401 aaatgatttt attcaaagcc atcaaactaa aactattatc aatattacac ataatttatc
     2461 cgatttaagt gctttgccgg gtaaatactt tatttttaaa gaccttcaaa tagaagagta
     2521 ccaatcaaaa gaagaagtca taaatgatta cattaattta taattgaggt gtccactttg
     2581 aaattagaat ttaaaaaaag tatttccaat aaaattattt acacgcttgg tgtgctattc
     2641 atattccttt tcttacttgg gtatttttta ccaataggaa tagacaaagt taaaagttta
     2701 agctatagcc agtttttctt cagttcatat actgttgcaa cgcaattagg gttcttacta
     2761 ttttcatttg ttattgcata ttttattaat aaagagtatt cgaataaaaa tatattattt
     2821 tacaagctga ttggcgataa tatctttacc ttcttctata aaaaggtagc agtgttattt
     2881 tttgaatgtt tagtgttcat aattctaagt atcactatta tttcaattat atattcggat
     2941 ttctcacatt acttactatt aataatctta ttttcactcg ttatcttaca atatatctta
     3001 gttgttggaa caattagtat ggtttcacct aacattttaa tcagcttagg tatcagtatt
     3061 gtatattgga ttggttcagt catcttagtt gccattaata aaaatatatt tggtatcgtt
     3121 gctccatttg aggcgagcaa cacgatgtat cgtgccgtag aaaaaatatt gaataacgag
     3181 tcaacattta tatgtcctac tgaaattata aatacagtat cattcttcgt tcttctattt
     3241 atagttaata ctattgtctt attactaagt agaaagagat ggttgaaaat cggtatgtag
     3301 tacataaaaa ggagcggcat ccattgtcgc tcctttttat tatgtatttt tacaatctcc
     3361 accaatgaca cgtcgtcatt gttacctcag accaaacaaa aaaacagtct cgcaatcaag
     3421 actgttttcc actcaatata ttcatccatt agcgtaatgg attatttaac ttctgtagct
     3481 acaaagattt tacgtgtttc ccaaacgcct gtcttttcat tgtaatcatc acaagtaatt
     3541 aatgttaatt gtttatcttt acctttttgt tcatctagaa cttctacatc tgtaggctta
     3601 acatctctta tacttgtcat tttatactta cgtgtttcat taccaacttt aaagtacacc
     3661 atactacctt ttttggctgc tttaagattt gtaaattgat agttcggacg gtcaatgaaa
     3721 gtgtgccctg caattgaaat attttgatca tctagtgatt cgttttcttc tgcaaagctt
     3781 acacctctat ttaattgttc aggtgttgct ggtcctggat atactggttc tttaatatca
     3841 gcatctggaa tttcaatata gcctgccact ttcgatttat ctttcggaat ctgaggttta
     3901 gcttgctgct ttttatcctt actcgcctgt tcttttacat ttttatcata ttgttcaatc
     3961 ttttcatctt tatctttatc gtgaagataa ttatcgatat gtggtttagc aaacaaatat
     4021 gctgccacta ggataagtac cacaccagcg attgtcatta atcgatttgt ccattttttc
     4081 atacgttaag gctcctttta tacatttcac aatgcctatt aacatttcat atagtatacc
     4141 attaatttca aagtgactca tagcaattca ttttatacta taaaatttac atgtatgctt
     4201 ttacgttaga tttcattaca catatttgca ttcaaataac gaaacgcttt taataattac
     4261 taagggggaa ttgttatgat tagatgcgct aaaaaagagg atttaaacgc tatattagtg
     4321 atatacaatg atgccattat caatactaca gctgtttata cttatgaacc acaaaccata
     4381 gacgaacgtg tcgcatggtt tgaaacgaaa caacgtaagc atgagcctat ctttgtattt
     4441 gaggaaaatg gaagtgtctt agggtttgcg acgttcggtt cgtttagacc ttggccagca
     4501 tacctatata caatcgaaca ttctatttat gtcgatgctt cagctagagg aaaaggtatt
     4561 gctagtcaat tactacacca tttaattgtg gaagcaaaag ctaaaggtta tcgtgcgcta
     4621 gttgcaggca ttgatgcttc caacaaagcg agtattcagt tgcatcaaaa gtttgctttt
     4681 aagcatgccg gcacactgac caatgtaggt tttaaattta atcaatggtt agatttagca
     4741 ttttacgaat tagatttaca agactagtaa tgtttgaatc acataatata aacaagacga
     4801 ccatgtcaat tcacttaaca taacaagcca acatataaaa ttttaaactt ctcagggaca
     4861 atttacatcg tcccttttat tttttctttt caaatactat ccccatagct ttgatttaac
     4921 gctttttctc aataacaaaa cgaatatagt agaacatgaa aacgataatc atgctgagcg
     4981 ataaagattt aaataataga ttgacccacg ttccctcagt cgtatatcca tatgtaatcg
     5041 ttgtgttaat gatgaatgct ataaagatga ttgatagtct tagcatatca tcactccttt
     5101 taagttattt tagatatacg ggggcgcttt tgcaatcact attttgatta gcatgtattt
     5161 cccataaatt tttcaacttc ttcagagata attaagaagc atctatctgg tactaatgat
     5221 ccagacagat gcttcttttt tatcaatatc ttattgttat ctcattaatt atttttaacc
     5281 atatcttcag ctgtgccaaa gattttacgt ttaattgctt cgccagttgg tgtgcctgct
     5341 agtccaccca atccagtttc acgtaatgat gcaggaaggt tacgaccaac cttatccatt
     5401 gcttcaataa cttcatcaac aggtattcta ctttcaatac ctgctaatgc taaatctgct
     5461 gaaattaaag cgttacccga accaattgca tttctcataa cacaaggaat ttcaacaagt
     5521 ccggctactg ggtcacaaac taatcctaat aaattgctta tcgctaatgc catagcgtgc
     5581 ccggatgctt ctggtgatcc tccgaatata gctactgctg cagctgccgc cattgcagat
     5641 gctgaaccaa cttcagcttg gcagccacct gttgcaccag ctacacttgc attgtttgct
     5701 acgacacgcc caaacaacgc tgaagtgaat aagaaatcaa tcatttgctc ttctgttaaa
     5761 tcatgtgttt tttctaattt aaaaagtgca ccgggaatgg tacccgagga accagctgtt
     5821 ggcgttgcac aaataatacc catcgcagca ttgacttcat ttgttgcaat ggcacctttg
     5881 actgcgtcaa tcatttcata tccagacaaa gcatgatgtg tttcattata atcacgtagt
     5941 ttagcagcgt catgaccagt gtagcccgtt acactttcaa cgccatcacc tgtcgtccct
     6001 ttgattactg cgtctcgcat gacatctaaa ttttgtttca tttgcgctcg cacttcatca
     6061 cgtgatttac cgcttaattc catttcttct ttaaccatga tatccgcaaa tgatatatta
     6121 ttttctacgg cataatctat agtctctcta attgaatcaa acatgtttat tccccctcta
     6181 atttatatag gaaacgttta cgtcactgta tttctcttta attgtattta atgtcgattc
     6241 tgagattgct ttatttaatg gtattacaac caagcattta tcttcatcta tcttaataaa
     6301 ttcatcttta cagtctaatt tcatatcgtt gatatcatta atgaaatgat ttacttgtgc
     6361 tttagtcata tttccgtcga caactaaaat tggtaatcca tgatttaaat ctacttctag
     6421 tccatttata tgaatacctt taattttaat tgtaccacca ccgattgaaa taccgataat
     6481 ttcaatgtag cgaccatcat tacgagatga tttaatataa gcacagtttg gatgttgacc
     6541 aatactatcg ccttcttctt cgatgatatc tattttaata ccatcatcag ctgcaatttc
     6601 taatgaagat ttaattcggt tatcaaatgt tgaatatccc attgctccac ccacaatagc
     6661 gacatctgta ccatgtcctt ggtgtgtttg agcaaatgat tcataataat gtatttcaat
     6721 atttttagga atatctccca atattgcgcg tgctgaattc ccaatcttta ctgcaccagc
     6781 cgtatgagaa cttgaagggc ccatcataac tggtccgata atatcgaaag cactttgata
     6841 atcatagctc tttgccataa ttaaacactc tccttaatat gattcttttt gctcggcatt
     6901 ttaaagttga tattcattaa attaaactta ttaattagtg ttccaataat ataggctaag
     6961 acaatgctga caataatcac tgttgcgatt gttacaattg atgtcactgc attattaaag
     7021 ccaaacaata cgatggcgcc tgcaattggt gttgccatac ctttgacacc tattactagt
     7081 ccgctaaatg tcacgataca tgcgttgacg accccaatca gtgcatttgt accatatagt
     7141 tgtactggat attgcgctat taaatcaatt tgcgtcaatg gctcaataca aactgcaaat
     7201 gcttttgacg gtccaccaat gtttaatttt cggaataaaa taaggttaac aaatgagcta
     7261 cctgtacatg ttagtgctcc aatagccata ggaacacctg tcagtcctaa taaacttgtt
     7321 aataccattg aacttagcgg tgtcatacct gtaacaggaa tcactagtcc taaaatgacc
     7381 gctaatgcat atggattgtt atcacctacc gcagtgacag cactacctat ttgttttaat
     7441 gttgctagca caccaggtgt aatgattgat gcaagtccga aagcaattgc tggtgcaaat
     7501 aagatcacca caattaagtc caagccttct ggaactttct tttcaatcca tttaattaaa
     7561 aaagctacgc cataagctgc gataaatgct ggtaataatt taaagtcatg taatactaaa
     7621 ccaacaatga ccgcaaatac tggtgcaacg cctaagttta aacacgttaa aatacctact
     7681 gcaataccgc ttaaacttcc tgctaaatcc ccaatatctt gtagaaattt aatatcaaat
     7741 acgccaccaa tagcataact taagaatgct tgtggtagaa atgtcgcaca agctgcaccg
     7801 gataatgctt gtagtccttg tttaccgtac ggtgcatact ttaaaaatag cgtcatgatc
     7861 actaaaacca agactaatgt gcctacacct aacagaatat ccatttccca aaaacctctc
     7921 tctatgttta ttttattttc agaccataaa catcgtacac ccttaagaaa acgttttcaa
     7981 cttttatctg ttatcaaatc aaatatttaa gtgaaatatt tcatatttgt gaaagatttt
     8041 taaaatggat tgtttcaaaa actacttatg ttgtcattaa tatttactaa ttaacttttt
     8101 cactctatat ttcaaacagt tgtgtgaaag ttttttgata acttttttac atctgaaagt
     8161 gagtaatatt tctaaaaatt tttaatattt atacatttta tctttcgagc tatttatttt
     8221 tgtttttgtg ctgtgttta
//
LOCUS       DAHHOJ010000111         7718 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.111, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000111 DAHHOJ010000000
VERSION     DAHHOJ010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7718
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.111"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            206..1126
                     /gene="mvk"
                     /locus_tag="NBH32_002219"
     CDS             206..1126
                     /gene="mvk"
                     /locus_tag="NBH32_002219"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HCD1515795.1"
                     /translation="MTRKGYGESTGKIILIGEHAVTFGEPAIAVPFNAGKIKVLIEAL
                     ESGNYSSIKSDVYDGMLYDAPDHLKSLVNRFVELNNITEPLAVTIQTNLPPSRGLGSS
                     AAVAVAFVRASYDFLGKSLTKEELIEKANWAEQIAHGKPSGIDTQTIVSGKPVWFQKG
                     HAETLKTLSLDGYMVVIDTGVKGSTRQAVEDVHKLCEDPQYMSHVKHIGKLVLRASDV
                     IEHHNFEALADIFNECHADLKALTVSHDKIEQLMKIGKENGAIAGKLTGAGRGGSMLL
                     LAKDLPTAKNIVKAVEKAGAAHTWIENLGG"
     gene            1131..2114
                     /gene="mvaD"
                     /locus_tag="NBH32_002220"
     CDS             1131..2114
                     /gene="mvaD"
                     /locus_tag="NBH32_002220"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HCD1515796.1"
                     /translation="MIKSGKARAHTNIALIKYWGKKDEALIIPMNNSISVTLEKFYTE
                     TKVTFNDQLTQDQFWLNGEKVSGKELEKISKYMDIVRNRAGIDWYAEIESDNFVPTAA
                     GLASSASAYAALAAACNQALDMQLSDKDLSRLARIGSGSASRSIYGGFAEWEKGYSDE
                     TSYAVPLESNHFEDDLAMIFVVINQHSKKVPSRYGMSLTRNTSRFYQYWLDHIDEDLA
                     EAKAAIQDKDFKRLGEVIEENGLRMHATNLGSTPPFTYLVQESYDVMALVHECREAGY
                     PCYFTMDAGPNVKILVEKKNKQQIIDKLLTQFDNNQIIDSDIIATGIEIIE"
     gene            2127..3203
                     /locus_tag="NBH32_002221"
     CDS             2127..3203
                     /locus_tag="NBH32_002221"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HCD1515797.1"
                     /translation="MIQVKAPGKLYIAGEYAVTEPGYKSVLIALDRFVTATIEEADQY
                     KGTIHSKALHHNPVTFSRDEDSIVISDPHAAKQLNYVVTAIEIFEQYAKSCDIAMKHF
                     HLTIDSNLDDSNGHKYGLGSSAAVLVSVIKVLNEFYDMKLSNLYIYKLAVIANMKLQS
                     LSSCGDIAVSVYSGWLAYSTFDHEWVKHQIEDTTVEEVLIKNWPGLHIEPLQAPENME
                     VLIGWTGSPASSPHFVSEVKRLKSDPSFYGDFLEDSHRCVEKLIHAFKTNNIKGVQKM
                     VRQNRTIIQRMDKEATVDIETEKLKYLCDIAEKYHGASKTSGAGGGDCGITIINKDVD
                     KEKIYDEWTKHGIKPLKFNIYHGQ"
     gene            3380..3721
                     /locus_tag="NBH32_002222"
     CDS             3380..3721
                     /locus_tag="NBH32_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1450 domain-containing protein"
                     /protein_id="HCD1515798.1"
                     /translation="MKNTFLICDECQAVNIRTLQKKLEKLDPDAEIVIGCQSYCGPGR
                     RKTFTFVNNRPLAALTEEELIEKVSQQLKKPRDSEEEERLRKRHEERKRRKEEQDRKL
                     KEKLEKRKAQQ"
     gene            complement(3773..5095)
                     /locus_tag="NBH32_002223"
     CDS             complement(3773..5095)
                     /locus_tag="NBH32_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HCD1515799.1"
                     /translation="MKTYDLIVIGFGKAGKTLAKYAASTGQHVAVIEQSSKMYGGTCI
                     NIGCIPSKTLVHDGLEGKSFEASYNRKNDVVNALNNKNYHLLADDNNIDVLDFKAQFK
                     SNTEVNLLDQHGDIADSITAPHIIINTGATSVIPNIKDLDQAKHVFDSTGLLNINYQP
                     KHLVIVGGGYIALEFASMFANLGSKVTVLERGESFMPREDQDVVAHAITDLENKGIAL
                     HTNVETTELSSDDHHTTVRTNVGNFEADAVLLAIGRKPNTDLALENTDIELGDRGEIK
                     VNAHLQTTVPHIYAAGDVKGGLQFTYISLDDYRIIKSALYGNQSRTTDNRGSVPYTVF
                     IDPPLSRVGLTSKEAAAQHYDYTEHQLLVSAIPRHKINNDPRGLFKVVINNENNMILG
                     ATLYGKQSEELINIIKLAIDQNIPYTVLRDNIYTHPTMAESFNDLFNF"
     gene            complement(5082..5522)
                     /locus_tag="NBH32_002224"
     CDS             complement(5082..5522)
                     /locus_tag="NBH32_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006189740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HCD1515800.1"
                     /translation="MNLEFNIAVHVLAFLTKHHSEKFNSSSLAELTCLNPVQLRRVTT
                     QLVDLKMINTIRGKDGGYLANDQSADVSLATLYKHFVLEKKHHTRLFTGDEGSHCQIA
                     RNIATTMSHYQQDEQNIIINFYNGKTIKDVIEDIQKEDLCHENI"
     gene            5580..5681
                     /locus_tag="NBH32_002225"
     CDS             5580..5681
                     /locus_tag="NBH32_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515801.1"
                     /translation="MCQYLCLKSVELGVVIVMIYCLKVVALTVRLHN"
     gene            6146..7540
                     /locus_tag="NBH32_002226"
     CDS             6146..7540
                     /locus_tag="NBH32_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1515802.1"
                     /translation="MSIDMYLDRSRNQASSVGNLSQTMNSNYDALEKAITQFINDDAL
                     KGKAYTSAKQFFSTVLIPLSTSMKTLSDLTKQACDNFVSRYTSEVDSISLKESELEED
                     IRSLSQQITRYENLTNNLKKHASDNQQAISSNQQIIQTLGQQKHELEEKLRKLREFNQ
                     KSPEIFKEVEEFQKIVQQGLTQAQNFWNFSTNQFNIPSGKELDWAKASHEKYLKVAMG
                     KIEHKAEKETLNKADFAVIKAYAKEHPEDDIPKSIMKYINDNKDSIKRDIGLDITSTL
                     LEQGGINASKFGVFINTAGGVKGPAGPNSFVEVKRTSGNVFIENGSKFAKGGKYLGKG
                     VAGVGFGIGMYDDLANDDKTFGEALSHNGMTLAAGSVAAFGVAALVSNPVGWAVVGIL
                     TAGIIGSIVSDYLYQNNYFGIKDKVDWAGHKIDEGIDAVNKTAEKAVDSVGNTIGDAT
                     KSISNHINPMKWSW"
     gene            7541..>7718
                     /locus_tag="NBH32_002227"
     CDS             7541..>7718
                     /locus_tag="NBH32_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000923048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD1515803.1"
                     /translation="MLLCESKVINKNPKYRIIKYESEYLMIDLASNWIVFFFPFINWL
                     IPKTYVKITKNDYEK"
BASE COUNT         2721 a         1101 c         1510 g         2386 t
ORIGIN      
        1 gaagtaaacg aaagttagtt ttatactaat caagttaagt caaatataaa ttcaaaataa
       61 agattataca taatatgaat aatgtgagat aaagactgca atgaaatttg acgcgaatgc
      121 gtatgcaact tagaatatga tgtcttttac taataaataa aaataatgaa tcaagtatag
      181 gataaatata aaaggtgata ttgacatgac aagaaaagga tatggggaat cgacaggtaa
      241 gattatttta ataggagaac atgctgttac atttggagag cctgctattg cagtaccgtt
      301 taacgcaggt aaaatcaaag ttttaataga agccttagag agcgggaact attcgtctat
      361 taaaagcgat gtttacgatg gtatgttata tgatgcgcct gaccatctta agtctttggt
      421 gaaccgtttt gtagaattaa ataatattac agagccgcta gcagtaacga tccaaacgaa
      481 tttaccacca tcacgtggat taggatcgag tgcagctgtc gcggttgctt ttgttcgtgc
      541 aagttatgat tttttaggga aatcattaac gaaagaagaa ctcattgaaa aggctaattg
      601 ggcagagcaa attgcacatg gtaaaccaag tggtattgat acgcaaacga ttgtatcagg
      661 caaaccagtt tggttccaaa aaggtcatgc tgaaacattg aaaacgttaa gtttagacgg
      721 ctatatggtt gttattgata ctggtgtgaa aggttcaaca agacaagcgg tagaagatgt
      781 tcataaactt tgtgaggatc ctcagtacat gtcacatgta aaacatatcg gtaagttagt
      841 tttacgtgcg agtgatgtga ttgaacatca taactttgaa gccttagcgg atatttttaa
      901 tgaatgtcat gcggatttaa aggcgttgac agttagtcat gataaaatag aacaattaat
      961 gaaaattggt aaagaaaatg gtgcgattgc tggaaaactt actggtgctg gtcgtggcgg
     1021 aagtatgtta ttgcttgcca aagatttacc aacagcgaaa aatattgtaa aagctgtaga
     1081 aaaagctggt gcagcacata cttggattga gaatttagga ggttaatgcg ttgattaaaa
     1141 gtggcaaagc acgtgcacat acgaatattg cacttataaa atattggggt aaaaaagatg
     1201 aagcactaat cattccaatg aataatagca tatctgttac attagaaaaa ttttacactg
     1261 aaacgaaagt cacttttaac gaccagttaa cacaggatca attttggttg aatggtgaaa
     1321 aggttagtgg caaagaatta gagaaaattt caaaatatat ggatattgtc agaaatagag
     1381 ctggcatcga ttggtatgca gaaattgaaa gcgacaattt tgtaccaaca gcagcagggt
     1441 tggcttcatc ggcaagcgca tatgcagctt tagcagcagc ttgtaatcaa gcgctagaca
     1501 tgcagctgtc agataaggat ttatcgagat tggcgcgaat tggttcgggt tctgcgtcgc
     1561 gtagtattta tggtggattt gcagaatggg aaaaagggta tagtgatgag acgtcatatg
     1621 ccgttccact tgaatcgaat cattttgaag atgaccttgc catgatattt gttgtgatta
     1681 atcaacattc taaaaaggta cctagtcgat atggtatgtc attgacacga aacacatcaa
     1741 ggttttatca atattggtta gatcatattg atgaagattt agctgaagca aaagcagcga
     1801 ttcaagacaa agattttaaa cgtcttggtg aagtaattga agaaaatggt ttgcgtatgc
     1861 atgccacgaa tctaggatca acaccgccgt tcacatatct tgtgcaagaa agttatgatg
     1921 taatggcgct cgttcacgaa tgccgagaag cgggatatcc gtgttatttt acgatggatg
     1981 cgggtcctaa tgtgaaaata cttgtagaaa agaaaaacaa gcaacagatt atagataaat
     2041 tattaacaca gtttgataat aaccaaatta ttgatagtga cattattgcc acaggaattg
     2101 aaataattga gtaaggaaga gataaaatga ttcaggtcaa agcacccgga aaactttata
     2161 ttgctggaga atatgctgta acagaaccag gatataaatc tgtacttatt gcgttagatc
     2221 gttttgtaac tgctactatt gaagaagcag accaatataa aggtaccatt cattcaaaag
     2281 cattacatca taacccagtt acatttagta gagatgaaga tagtattgtc atttcagatc
     2341 cacatgcagc aaaacaatta aattatgtgg tcacagctat tgaaatattt gaacaatacg
     2401 cgaaaagttg cgatatagcg atgaagcatt ttcatctgac tattgatagt aatttagatg
     2461 attcaaatgg tcataaatat ggattaggtt caagtgcagc agtacttgtg tcagttataa
     2521 aagtattaaa tgaattttat gatatgaagt tatctaattt atacatttat aaactagcag
     2581 tgattgcaaa tatgaagtta caaagtttaa gttcatgcgg agatattgct gtgagtgtat
     2641 atagtggatg gctagcgtat agtacttttg atcatgaatg ggttaagcat caaattgaag
     2701 atactacggt tgaagaagtt ttaatcaaaa actggcctgg attgcacatc gaaccattac
     2761 aagcacctga aaatatggaa gtacttatcg gttggactgg ctcaccggcg tcatcaccac
     2821 actttgttag cgaagtgaaa cgtttgaaat cagatccttc attttacggt gacttcttag
     2881 aagattcaca tcgttgtgtt gaaaagctta ttcatgcttt taaaacaaat aacattaaag
     2941 gtgtgcaaaa gatggtgcgt cagaatcgta caattattca acgtatggat aaagaagcta
     3001 cagttgatat agaaactgaa aagctaaaat atttgtgtga tattgctgaa aagtatcacg
     3061 gcgcatctaa aacatcaggc gctggtggtg gagactgtgg tattacaatt atcaataaag
     3121 atgtagataa agaaaaaatt tatgatgaat ggacaaaaca tggtattaaa ccattaaaat
     3181 ttaatattta tcatgggcaa taaatgactt gataggggag caatcatcta atttaattag
     3241 aagataaatg ctccattttt tattgccagc cgtgttacgt atcttgaaaa aaatattaaa
     3301 aagagtaaaa tagatagagt taacgaaaaa attaatgaaa tcgacgatat agatatgata
     3361 aagaaaggtg ggtagcaata tgaaaaatac attccttatt tgtgatgaat gtcaggcagt
     3421 caatataaga acgttacaaa agaagttgga aaaattagat cccgatgctg aaatcgtgat
     3481 aggttgtcaa tcttattgtg gacctggacg ccgaaaaaca ttcacttttg ttaataaccg
     3541 cccactggct gcgcttactg aagaagaatt aatcgaaaaa gtttctcaac aattaaagaa
     3601 accacgtgat tctgaagaag aagagcgttt aagaaaacga catgaagaac gtaaacgtcg
     3661 taaagaagaa caagatagaa agcttaaaga aaaattagaa aagcgaaaag cacaacaata
     3721 aaacctgatg gcagcatcat tcaatgcgtg ccaccaggtt tttatgtttt gtctagaaat
     3781 taaataaatc attaaatgat tcggccatcg taggatgtgt ataaatatta tctcgtaata
     3841 cggtatatgg aatgttttga tcaatcgcaa gtttaattat attaattaat tcttcagatt
     3901 gcttaccata taatgtagca cctaaaatca tattattttc attattaatg actactttaa
     3961 ataaacccct tggatcattg ttaattttgt gacgaggtat tgcacttact aaaagttggt
     4021 gttcagtgta atcataatgt tgagcggctg cttctttact agttaatcca acacgtgata
     4081 atggtggatc tataaatact gtataaggca cgctgcctct attgtcagtc gtacgtgact
     4141 gattaccata taacgctgat ttgataattc gataatcatc taaagatata tacgtaaatt
     4201 gaagtccgcc tttaacatca cctgcagcat aaatatgcgg cacagttgtt tgaagatgag
     4261 cattgacttt aatttcgcct ctgtcgccta attcgatatc agtattttct aaagctaaat
     4321 ctgtattcgg tttgcgtccg atagccaaaa gtactgcatc agcctcaaag ttgccaacgt
     4381 tagtacggac tgttgtatga tgatcgtcag atgacaattc agtcgtttca acatttgtat
     4441 gcaatgcaat gcctttattt tctaagtcag taatagcatg tgcaacgaca tcttgatctt
     4501 cgcgtggcat aaatgattca ccacgttcta atactgttac cttactacct aaattcgcaa
     4561 acattgaagc aaattctaag gcgatataac cgccacctac aataacgagg tgcttaggtt
     4621 gatagttaat gtttaataaa cctgttgaat cgaagacatg tttagcttga tcaaggtctt
     4681 ttatgttagg aatgacagag gtagcaccgg tattaataat gatatgaggt gcagtaatac
     4741 tatcggcgat atcgccatgc tgatctaata aattcacttc agtattagat ttaaactgcg
     4801 ctttaaaatc cagtacatca atgttgttat cgtctgctaa taagtggtaa tttttattgt
     4861 ttagcgcatt gacaacatcg tttttacgat tataacttgc ttcaaaggat ttgccttcta
     4921 atccatcatg tacaagtgtc ttcgaaggta tacatcctat attgatacaa gtgcctccat
     4981 acattttcga agattgttcg ataactgcga cgtgttgacc tgttgatgca gcgtatttcg
     5041 ctaaagtttt accagctttc ccaaatccta ttacaattaa atcatatgtt ttcatgacat
     5101 aaatcctcct tttgaatgtc ttcaatgaca tctttgattg tttttccatt ataaaaatta
     5161 ataatgatat tctgttcgtc ttgctgataa tgtgacatgg tagttgcaat attacgagca
     5221 atttgacagt gactgccttc gtcgccagta aatagacgtg tgtggtgttt tttctctaag
     5281 acaaaatgtt tatataatgt tgctagagag acatcagcac tttgatcatt tgctaaatat
     5341 ccaccatctt taccccgtat tgtgttaatc atttttaaat cgacaagttg agtcgtcacg
     5401 cgtcgtaatt gaacaggatt taaacaagtt aattcagcta atgaactact attgaatttt
     5461 tctgaatgat gcttagttaa aaaagctaat acatgcacgg caatgttaaa ttctaaattc
     5521 aatgtttctc acctcataat tgtaactgta ttagttacaa ttaaaaatca ttttgctcaa
     5581 tgtgtcaata cttatgtttg aaaagtgttg aattaggtgt ggttattgtg atgatttatt
     5641 gtttgaaagt tgttgcatta acggtgagat tgcacaattg aaattttagt tatgtatgaa
     5701 ctgttaaata tgaaatgaat attgaaatga gggcaataaa attttgaatg atgatgttgt
     5761 gtgattttgt aaacttattc gtattttaga tattttttga tatttcgcat gatgaggaag
     5821 tattttcaac aactggtgac atatttaaat cgtaaatata ttttgagata ttaatatata
     5881 ttagattaag gatgttgtcg caataaaatt aaaatattga aagataattt ttattgatta
     5941 attgtgaata tacaaagaca ccattatatc catatgctaa aataagaaaa aaggcgacat
     6001 gttatctcaa tttacctgca tctagacccc tttacataag aaataatcat cgctgagttt
     6061 taaaatttta caactttata gatgtaaaaa gcatttaatt ttaaatagtt tgaaaatcat
     6121 atatttttga aaaaggagca tgccgatgag tattgacatg tatttagaca gatctcgaaa
     6181 ccaagcttca agtgtgggta atttgagtca aacaatgaat tcaaattatg atgcgttgga
     6241 aaaagcaatt actcaattta tcaatgatga tgcgcttaaa ggtaaagcgt atacgtcagc
     6301 taaacaattt tttagtacgg tgttaattcc attatcaaca agtatgaaaa cattgagtga
     6361 tttaacgaag caagcttgcg ataattttgt gtcacgttat acgagtgagg ttgatagcat
     6421 atctttaaaa gaatcagagc ttgaagaaga tatcagatca ttaagtcaac aaattacgcg
     6481 atatgaaaat ttaactaaca atttgaaaaa gcatgcttcc gataatcagc aagccatttc
     6541 atcgaaccaa caaataatac aaacattagg tcaacaaaaa catgaattag aagagaagct
     6601 acgcaaattg cgtgagttta atcaaaaatc accagaaata tttaaagaag ttgaagaatt
     6661 tcaaaaaatt gtccaacaag gacttaccca agcgcaaaat ttttggaact tttcaacaaa
     6721 tcaatttaac attccttcag gtaaagaact tgattgggcc aaagcaagtc atgaaaaata
     6781 tttgaaagtt gctatgggga aaattgaaca taaagcagag aaagaaactt taaataaagc
     6841 agactttgct gttataaagg catatgccaa agaacatcca gaagacgata tcccgaaaag
     6901 tataatgaaa tatataaatg acaataaaga tagtattaaa cgagatatag gattagatat
     6961 tacgtcaaca cttttagagc aaggcggtat aaatgcaagt aaattcggtg tatttatcaa
     7021 tacagcaggt ggagtgaaag gcccagccgg tccaaattca tttgtggaag tcaaacgtac
     7081 atcaggtaat gtgtttatag aaaatggtag taaatttgct aaaggtggaa aatatctagg
     7141 taaaggtgtt gcaggtgtag gatttggtat aggtatgtat gatgaccttg caaatgatga
     7201 taaaacattt ggggaggcgt tgtcgcataa tggtatgacg cttgcagctg gatctgttgc
     7261 agcatttggt gtagcggctt tagtaagtaa tccagtcggt tgggctgtgg taggtatttt
     7321 aacagcaggt attattggat ctattgtttc tgattatctt tatcaaaata attattttgg
     7381 cataaaagat aaagtggact gggcaggaca taaaatagac gagggtattg atgctgtaaa
     7441 caaaactgca gaaaaagctg tagatagtgt tggtaatacc attggtgatg ccacaaaaag
     7501 tattagtaat catattaatc ctatgaaatg gagttggtaa atattgcttt gcgaatctaa
     7561 agttattaat aaaaatccta aatatcgaat aatcaaatac gaaagtgaat acttaatgat
     7621 tgatttagca agtaattgga ttgttttctt ctttccattt attaactggc tcataccgaa
     7681 aacatatgtc aaaatcacta agaatgatta tgaaaaat
//
LOCUS       DAHHOJ010000112         7696 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.112, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000112 DAHHOJ010000000
VERSION     DAHHOJ010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7696
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.112"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..321
                     /locus_tag="NBH32_002228"
     CDS             <1..321
                     /locus_tag="NBH32_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HCD1515804.1"
                     /translation="GGEKPKDPKGPENPEKPSRPTHPSGPVNPDNPGLSKDRAKPNEP
                     VHSMDKNDKVKKSKIAKKSVANQEKKRAELPKTGAESTQKGLIFSSIIGIAGLMLLAR
                     RRKN"
     gene            704..1897
                     /locus_tag="NBH32_002229"
     CDS             704..1897
                     /locus_tag="NBH32_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515805.1"
                     /translation="MLYLEVLKNRNFTYLLIGNFLRRSCFVLFSLQIIWFTVELTNQS
                     SLKLSMMVMSQTLPFIIFGIFGGAYSDKHNKKKILYLSDLILSLIIIIIPLLAITSNL
                     NYLTLLTISTAITIINCYTDPAFRAILPEIIDEEHLATSNALIDSLQRGSNIILPALI
                     GVIVILVGNVGVFFICSILLFLGFIFNALLKYTNNNMIDRHSKEDFSETWEFLKKSKE
                     IPFIIIIQFACILINTGLWRVALPLFISNILKEGVGVYGLATSCLGIASLLMSLIMGL
                     LSEKRLIFKFSIGVLVWGIGLSIINVFPSVAILYIGATLVGLGQSIEGLTRSVAIQIK
                     TPNHLMGKVFSISSTSNYAADTLSLGLISLIIPLLSLSNIFISGGAIISMLSLSGLKF
                     FKKSM"
     gene            1908..2111
                     /locus_tag="NBH32_002230"
     CDS             1908..2111
                     /locus_tag="NBH32_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515806.1"
                     /translation="MLINAIKVGIEMKYKISLAYNLAIIIGSLIILCILISRGYDIYV
                     ILIPILTILASLINLICDIKKHK"
     gene            complement(2219..2527)
                     /locus_tag="NBH32_002231"
     CDS             complement(2219..2527)
                     /locus_tag="NBH32_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515807.1"
                     /translation="MTVNIVFSIVFCISMVILGIYVAITKDFTLISYINQTTIADKHK
                     NQIAYIFTLCISLSAVFLMSSILCFEYDFIALSFLFLTIALLLIALFYVCFYKITKYP
                     "
     gene            complement(2671..2937)
                     /locus_tag="NBH32_002232"
     CDS             complement(2671..2937)
                     /locus_tag="NBH32_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515808.1"
                     /translation="MKGSKQILLIMGIISLIVLFIFTLFIMAQYAKHYEQKSDSSNAH
                     TLNSSSAIIEQHTMSNLASLDLYAPVRNITSSRDIHPIYFMTKD"
     gene            complement(3221..5029)
                     /locus_tag="NBH32_002233"
     CDS             complement(3221..5029)
                     /locus_tag="NBH32_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HCD1515809.1"
                     /translation="MHFHTHLLYNTCEKYCLGAVFFYFWGVFLYNSIHKCKGFSFHVL
                     LSLTEILKGCLATMDKELWIERANDSLVKHFYEQQSDIEQREGFESKLTFGTAGIRGK
                     FGLGEGRLNKFTIEKLALGLARYLNAQTNSPTIVIHYDIRHLSTEFAQIIANVLANHQ
                     ITVYLPDTYKTTPELSFAVRNLNTTAGIMITASHNPKDYNGIKVYGSDGAQLSTDASE
                     LASRYIEEVGDPLQIDIPSSKQNTSYIKPFPKSVTDGYMKHIQNMIGYIPKSDLQVVF
                     TSLHGTSVPIVPELLKSLNFNQFNLVEAQCKPDPNFSSVQSANPEDHRAFDKAVELAN
                     KSHANLLISTDPDADRLGIAERDAHGHITYFNGNQIGALLLNYRIQQTSQLRHRLMIQ
                     SIVSSELTKSLSRYNNVEYKEVLTGFKFIAQEIRQLDDHQNMIFAFEESYGFLSEPFV
                     RDKDAVQIVPLIIKYASELKLYGKTLKDELEQIYQTVGRHEDTLFSHTLEGFEGKKKI
                     NAIMTKFRSNPPQEIQGLKVKAIEDYLTSEVYHLDKDTTSQINSPKSNVIRVLFDEGF
                     IALRPSGTEPKIKLYVSLKCPNFDDVAQKINAMIFS"
     gene            5161..5553
                     /locus_tag="NBH32_002234"
     CDS             5161..5553
                     /locus_tag="NBH32_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(deoxy)nucleoside triphosphate
                     pyrophosphohydrolase"
                     /protein_id="HCD1515810.1"
                     /translation="MKKVINVVGAIIFSDNKILCAQRSEEMSLPLMWEFPGGKVEKNE
                     TEKDALIREIREEMKCDLIVGDKVITTEHEYDFGIVRLTTYKCTLNKELPTLTEHKSI
                     EWLSINELDKLNWAPADIPAVNKIMTEG"
     gene            5555..>7696
                     /locus_tag="NBH32_002235"
     CDS             5555..>7696
                     /locus_tag="NBH32_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCD1515811.1"
                     /translation="MSRLLNDFNQSLHKGFIDKDISHKGNYTPKLLVNNKNEKVLSTI
                     IDELQKCETFYFSVAFITESGLASLKAQLLDLSNKGVKGKILTSNYLGFNSPKMYGEL
                     LKLKNVEVRLTDIAGFHAKGYIFEHKDYSSMVIGSSNLTSNALKVNYEHNVLLSTMKN
                     GDLVDSVKNEFDLLWQKSIPLTEQWINSYKESFEYRSLEKLAEVEQTQMLLADKVKKS
                     VEIVPNLMQAEALRSLKAIRDKAKDKALIISATGTGKTILCALDVREVNPNKFLFIVH
                     NEGILNRAKEEFKKVLPIKNDSDFGLLTGKHRDVDAKYLFATIQTLSRDDNFKQFDEN
                     EFDYIVFDEAHRSAASTYQRVFNYFKPKFMLGMTATPERSDELSIFELFDYNIAYEIR
                     LQAALESDILCPFHYFGVTDYVHQGIKEDDVTKLRYLTSDERVNYIIQKTDYYGYSGE
                     ILQGLIFVSSKKEAYDLADKLSSKGIKSVALTGDDSVNYRQIVIEKLKEGKINYIITV
                     DLFNEGIDIPEVNQVVMLRPTESSIIFIQQLGRGLRKSANKEYVTVIDFIGNYKTNYL
                     IPIALSGDQSQNKDNYKKFLTNNDSINGVSTINFEEVAKKQIYNSLDAVSLNQNKLIL
                     KAYEEVENRLGHMPLLMDFIQQHSIDPSVIFSKFSNYYEFLVRYKKIDTLLTENESKN
                     LVFFSRQIAPGLKRIDSLVLEELLKNELTYDE"
BASE COUNT         2777 a         1003 c         1296 g         2620 t
ORIGIN      
        1 ggtggcgaga aaccaaaaga tccaaaagga cctgaaaacc cagagaagcc gagcagacca
       61 actcatccaa gtggcccagt aaatcctgac aatccaggat tatcgaaaga cagagcaaaa
      121 ccaaatgaac cagttcattc aatggataaa aatgataaag ttaaaaaatc taaaattgct
      181 aaaaaatcag tagctaatca agagaaaaaa cgagcagaat taccaaaaac aggtgcagaa
      241 agcacacaaa aaggattgat ctttagtagt ataattggaa ttgctggatt aatgttattg
      301 gcacgtagaa gaaaaaatta aaataattca ttatttaaat aatacttgat ttgcattcac
      361 tatatttagt ttgttaaaaa caacctagaa tatgatgaaa atgatataca ccccaaaagt
      421 tggattgaaa gacctaactt taaggggtgt atttttgtta taaatttgat tttttgataa
      481 tattttcata tttaatataa tcataattag tagaatataa cctctgagta gtcattttgg
      541 gggttatgta tggtgtttca agtagtatgt aaatttcgaa ataattgata tttaactgac
      601 taaattatat tagaattaat tgcgctttgg ataaagagag tttagaagaa ggtctaaaaa
      661 agctggtgga tgcattcaat tatttttatg agaggtgata aatatgttgt acttagaagt
      721 tttgaaaaat agaaacttta catatttatt gattggtaat ttcttacgtc ggagttgctt
      781 tgtgctgttt tcgttacaaa taatttggtt tactgttgaa ttgacaaacc aatcatcttt
      841 aaaattatca atgatggtta tgagtcaaac actaccattt attatatttg gtattttcgg
      901 tggcgcatat tctgataaac ataataaaaa gaaaatactt tatctatctg atcttatact
      961 aagtttgatt ataataatta ttcctttact tgcaataaca tcaaatttaa attatctgac
     1021 gcttctaacc atatctacag ctatcaccat tattaactgc tatacggatc ctgcgtttag
     1081 ggcaattctt cctgaaatta ttgatgagga gcatttagca acaagtaatg cgttaatcga
     1141 tagtttgcaa agaggctcaa atattatttt acctgcttta attggtgtca ttgtaatact
     1201 agttggtaat gttggcgtct tttttatttg cagtatattg ctatttttag gatttatttt
     1261 taatgcactt ttaaaatata caaataacaa catgattgat agacactcaa aagaagattt
     1321 ttctgaaact tgggaattct tgaaaaaatc taaagaaatt ccatttatta taatcattca
     1381 atttgcctgc atattgatta atactggcct ttggcgtgtt gcattaccat tgtttatttc
     1441 caatatatta aaagagggcg ttggtgtata tggactagct acatcatgtt taggtatagc
     1501 gtctttatta atgtcattga ttatgggttt actgtcagaa aaacgattaa tctttaaatt
     1561 tagtattggt gttttagttt ggggaattgg tttatcgatt attaatgtgt ttccaagtgt
     1621 ggcgatattg tatataggtg caacattagt gggacttgga caatctatag agggactaac
     1681 aagatcagtt gccattcaaa taaaaacgcc aaatcatttg atggggaaag tatttagtat
     1741 ctcatctaca tcgaattacg cagcggatac attatcttta ggactgataa gtttaattat
     1801 acctttacta agcttaagta atatatttat atcaggaggt gcaattattt caatgcttag
     1861 cttatcgggg ttaaagtttt ttaagaagtc tatgtaaaca taatagaatt ttaatcaatg
     1921 ctattaaggt gggaattgaa atgaaatata aaattagttt ggcttataat ttagcaatta
     1981 taatagggtc gctaattata ttatgtatac taatatctag aggttatgat atatatgtaa
     2041 tcttgattcc aattttgact attttagcta gtttaatcaa tttaatttgt gatattaaaa
     2101 agcataaatg attctatatt aatgcaactt tgtattcgta caaggttgta tttttttcgc
     2161 aaacaattac ttaatgacag cgcatcaata aatctaacca acacactatc gcaaacattt
     2221 atggatattt tgtgatttta taaaagcaaa cgtagaataa tgcgataagt aataatgcta
     2281 tcgttaaaaa taagaatgac aatgcaataa aatcatattc aaagcataga attgaagaca
     2341 ttaaaaatac tgcactcaaa ctgatacata gcgtaaatat atatgcaatt tggttcttgt
     2401 gtttatctgc aatggtcgtt tgatttatat aagaaattag tgtgaaatct ttagttattg
     2461 cgacataaat gcctaatata accatactta tgcagaatac gatactaaat acgatgttga
     2521 cggtcattgt tgcaccttcc tgatagaata taatgtactg tcttttagta atgaatgcgt
     2581 atcttatata tgtatgtttc taaaaaacag tacgttttta tcaattttta atttattagt
     2641 tgaatgaatg gggttatgaa aaaggaagtt ttaatctttt gtcatgaaat aaatgggatg
     2701 aatatcacga ctagaagtaa tgttacgaac aggagcgtat aaatctagag acgctaaatt
     2761 cgacatagta tgttgctcaa ttatggctga tgatgagttt aaagtatgtg cgttggaact
     2821 gtcggatttt tgttcataat gttttgcata ttgtgccatg atgaatagtg taaaaataaa
     2881 taaaacaata agagatataa tgcccataat caaaagtatt tgtttagagc ctttcattat
     2941 ttcacatcct ttctaaaata tatttgtaac taaatttaaa atagttattt ttgtaattct
     3001 aaaccttttt catcgcgaaa acaattaaat aggtcgcggt attaattatt atattattac
     3061 cgcttaatat gaaaaataca tgaaaattaa ttttctaata tacttttgaa aaattattac
     3121 aaattagccc cttcaaaacg cgaaaacata aggattctag tttcaaaagg gctgataagt
     3181 ataaaatgaa atgtaatatt tcgatgttta aaatttttaa ttagctaaaa atcatcgcat
     3241 taattttttg agctacatca tcaaaattcg gacattttaa cgacacatat aatttaattt
     3301 taggttcagt accagaagga cgtaaagcga taaatccttc gtcaaataag acacgaataa
     3361 catttgattt aggagaatta atctgcgacg ttgtgtcttt atccaaatga taaacctcgc
     3421 tagttaaata atcttcaatt gctttcactt tcagtccttg aatctcttgc ggtggatttg
     3481 aacggaattt ggtcattatt gcattaattt tctttttccc ttcaaatcct tctagcgtat
     3541 gcgagaataa tgtatcctca tgtctaccaa cagtttgata aatctgttct aattcatctt
     3601 tcaatgtttt gccatataat tttaactcag aagcgtattt tataatgagt ggcacaattt
     3661 gtacggcatc tttatcacgt acaaaaggct ctgatagaaa accgtaactc tcttcaaatg
     3721 cgaaaatcat attttgatga tcatccagtt gtcttatttc ctgagcaata aatttaaagc
     3781 ccgtcagcac ctctttgtat tcaacattat tatagcgtga taatgacttt gttaactcac
     3841 tacttacaat agattgaatc attaagcggt gacgtaattg agacgtttgt tgaatacgat
     3901 aattaagtaa cagcgcacca atttggttgc cattaaaata cgtgatatga ccatgtgcat
     3961 cacgttcagc aatgccaaga cgatccgcat caggatcagt tgatattaat aagtttgcat
     4021 gacttttatt tgcaagttcg actgctttat caaaagcgcg atgatcttca ggattagcac
     4081 tttgaactga gctaaaattc ggatcaggtt tacattgtgc ttctacaaga ttaaattgat
     4141 tgaaattcaa agattttaat aactcaggta caatcggaac acttgtacca tgtaaactag
     4201 tgaacacaac ttgtaaatca gatttaggaa tatagccaat catattttga atgtgtttca
     4261 tatagccatc agttacagat ttcggaaatg gcttgatata agatgtattt tgttttgaag
     4321 aaggtatgtc aatctgtaat ggatcgccaa cttcttcgat ataacgactt gcaagctcag
     4381 atgcatctgt tgatagttgc gcaccatcag aaccatatac tttaatgccg ttatagtctt
     4441 tcggattatg acttgctgta atcataatgc cagcagtagt attaagattg cgcaccgcga
     4501 acgataattc cggtgtcgtt ttatatgtat caggtaaata aactgttatt tgatggtttg
     4561 ctaatacatt agcaataatt tgggcgaatt cagttgaaag atgtctaata tcataatgaa
     4621 tgactattgt tggactgttt gtttgggcat ttaaataacg cgctaaacct aatgccaatt
     4681 tttcaatagt aaacttatta agtcgacctt caccaagacc gaattttccg cgtatacccg
     4741 cagtaccaaa tgttaattta ctttcaaaac cttctcgctg ttcaatgtca gattgctgct
     4801 cataaaaatg tttaactaaa ctatcattag ctctttctat ccataattct ttatccattg
     4861 ttgctaaaca tcctttcaaa atctcagtta gacttaataa aacatgaaaa ctaaagccct
     4921 tacatttatg tattgaatta taaagaaata cgccccaaaa gtaaaaaaac acagccccaa
     4981 gacaatactt ttcacaagta ttatataata gatgtgtatg aaaatgcatg gagtagatgt
     5041 aagagtgata tatcatgcag gaaaatgtaa aatattaatt tttaatattg atttatatat
     5101 agttttgtaa tttttgatag aataacaatt aagatgattt taaattgaag ggtgagtctt
     5161 atgaaaaaag taatcaatgt agtaggagct attatttttt ctgataacaa aattctttgt
     5221 gcacagagaa gtgaagaaat gagtctgcct ttaatgtggg aatttcctgg aggtaaggtt
     5281 gaaaagaatg aaactgaaaa agacgctttg attagagaaa ttagagaaga aatgaaatgt
     5341 gatctaattg ttggagacaa agttataact acagaacatg aatatgattt tggaattgtt
     5401 aggttaacaa catacaaatg tactttaaac aaagagttac caactctaac tgaacataag
     5461 agtattgaat ggttgtcaat aaacgaactt gataaattaa attgggcccc agcggatata
     5521 ccagccgtta ataaaattat gaccgaggga taatatgagt agattactaa atgatttcaa
     5581 tcaatcttta cataaagggt ttatagacaa agatatttct cataaaggta attatacacc
     5641 gaagctttta gtaaataata aaaatgaaaa agttctttca acaattatag atgaacttca
     5701 aaaatgtgaa acgttctatt tttctgtagc ttttataaca gaaagcggtt tagccagctt
     5761 aaaggctcaa ctattagatt taagcaataa gggtgttaaa gggaaaatat taacatctaa
     5821 ttacttaggg tttaatagtc ctaaaatgta tggagaatta cttaaattaa aaaatgtaga
     5881 ggtaagatta accgatattg ctggattcca tgccaaagga tatatttttg aacataagga
     5941 ttatagttct atggtaatag gaagctctaa tttaacatct aatgcgttaa aggttaatta
     6001 tgagcataat gttttactgt ctactatgaa aaatggcgat ttagttgata gtgtaaaaaa
     6061 tgaatttgac ttgttatggc aaaagagtat cccactgact gagcaatgga ttaattcata
     6121 taaagaatca tttgagtacc gttccttaga aaaattagcg gaggttgaac aaactcaaat
     6181 gctgctagct gataaggtga aaaaatcagt ggaaattgta cccaatttaa tgcaagcaga
     6241 agcattaagg tcattaaaag ctattaggga taaagccaaa gataaggcgt taataatatc
     6301 tgcaactggt acaggtaaaa cgattttatg tgcattagat gttagagaag taaaccctaa
     6361 taaattttta tttattgttc ataatgaggg gattttaaat agagctaagg aagaatttaa
     6421 aaaagtatta cctataaaaa atgatagtga ttttggattg ttgacaggaa aacatagaga
     6481 tgtcgatgcc aaatatttat ttgcaaccat tcaaacatta tctagagatg ataattttaa
     6541 gcaatttgat gaaaatgaat ttgattatat tgtttttgat gaagcacatc gttctgctgc
     6601 atctacttat caacgagtat ttaattactt caaacctaag ttcatgctgg gaatgactgc
     6661 tacaccagaa agatcagatg aattaagtat atttgaactg tttgattata atattgcata
     6721 tgaaataagg ttacaagcag cgttagaaag tgatatttta tgtccatttc attattttgg
     6781 tgtgactgat tatgtacatc aaggtattaa agaagatgat gtaactaaac tgagatattt
     6841 aacttctgat gaaagagtta attatattat tcaaaagaca gattactatg gatattcagg
     6901 tgaaatttta caaggattaa tttttgtgag tagcaaaaaa gaagcttatg atttagcgga
     6961 taaactaagt tcaaaaggga ttaaaagtgt tgctttaaca ggagatgata gtgttaatta
     7021 tcgccaaatt gtaatagaaa aattaaaaga aggtaagatt aactatatta taactgtaga
     7081 tctttttaac gaaggtattg atattccaga agtcaatcaa gttgtaatgt tgagaccaac
     7141 agagtctagt attatattca ttcagcaact tggtagagga ttaagaaaaa gtgcaaataa
     7201 agagtatgtt actgtaattg attttattgg taattataag accaattatt taattccgat
     7261 tgcgctttct ggagatcaat cgcaaaataa agataattat aagaagtttt taacgaataa
     7321 cgattcgatt aatggagtat ctacaattaa ttttgaagaa gttgctaaaa aacagattta
     7381 caattcattg gatgcagtat cattgaatca aaataaatta atattaaaag cttatgaaga
     7441 agttgaaaat agattgggac acatgccgtt actaatggat tttatacaac aacattctat
     7501 agatccaagc gttatatttt ctaaatttag taattattac gagtttttag taaggtataa
     7561 aaaaatagat actttattga cggaaaatga atctaaaaat ctggttttct tttcaagaca
     7621 aatagcacct ggattgaaaa gaatcgatag tttagtttta gaagaacttt taaaaaatga
     7681 attaacatat gatgaa
//
LOCUS       DAHHOJ010000113         7598 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.113, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000113 DAHHOJ010000000
VERSION     DAHHOJ010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.113"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            91..996
                     /gene="yut"
                     /locus_tag="NBH32_002236"
     CDS             91..996
                     /gene="yut"
                     /locus_tag="NBH32_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016266007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HCD1515812.1"
                     /translation="MKMIDVLLKNISQVVLISNKWTGLFILIGLFVADWTIGLAAIVG
                     SIIAYTFARFINYSEAEINDGLAGFNPVLTAIALTIFLDKSGLDIVITMIATLLTLPV
                     AAAVREVLRPYKVPMLTMPFVIVTWFTILLSGQVKFVDTSLKLMPQNIETVNFSNNDR
                     IHFIQSLFEGFSQVFIEASVIGGVCILIGILIASRKAALLAVIASLLSFIIVALLGGN
                     YDDINQGLFGYNFVLMAIALGFTFKTEINPYISTFLGVLLTVVVQLGTTTLLEPFGLP
                     ALTLPFIIVTWILLFAGIKHDKVDA"
     gene            1213..2367
                     /locus_tag="NBH32_002237"
     CDS             1213..2367
                     /locus_tag="NBH32_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA/acyl-ACP dehydrogenase"
                     /protein_id="HCD1515813.1"
                     /translation="MNDMDNSFLITTEIQRKWIEKFKVIGDTFKAKAEYNDQHSQFPY
                     KNIEWLIKEGYGKLTLPKEYGGEGATIEDMVILQSFLGELDGATALSIGWHVSVVGQI
                     YEQKLWSQDMLEQFAVEINNGALVNRAVSEAEMGSPTRGGRPSTHAVKADDGYILNGV
                     KTYTSMSKALTHIIVAAYIEELESVGFFLVDRNLPGVEIADNWDVLGMRATESHDLIL
                     NDVKVPLKHLVETEKSKAPNGWILHIPSCYLGIAQAARNYAVDFAIQHSPNSIEGTIA
                     TLPTVQQNLGKMETLLLCARQFLWSTAKGYQQYTDESQIRNATSASKVMVMNQGLEVI
                     DLAMRIVGAKSLEMNRPLQRYYRDMRAGLHNPPMEDAAYTNIAKSITDTF"
     gene            2580..3488
                     /locus_tag="NBH32_002238"
     CDS             2580..3488
                     /locus_tag="NBH32_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCD1515814.1"
                     /translation="MKKLLLPLIIMLLVLAACGNQGEKNNKAETKSYKMDDGKTVDIP
                     KDPKRIAVVAPTYAGGLKKLGANIVAVNQQVDQSKVLKDKFKGVTKIGDGDVEKVAKE
                     KPDLIIVYSTDKDIKKYQKVAPTVVVDYNKHKYLEQQEMLGKIVGKEDKVKAWKKDWE
                     ETTAKDGKEIKKAIGQDATVSLFDEFDKKLYTYGDNWGRGGEVLYQAFGLKMQPEQQK
                     LTAKAGWAEVKQEEIEKYAGDYIVSTSEGKPTPGYESTNMWKNLKATKEGHIVKVDAG
                     TYWYNDPYTLDFMRKDLKEKLIKAAK"
     gene            3886..4827
                     /locus_tag="NBH32_002239"
     CDS             3886..4827
                     /locus_tag="NBH32_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HCD1515815.1"
                     /translation="MTKKVYFNHDGGVDDLVSLFLLLQMENVQLIGVSTIGADCYLEP
                     SLSASVKIINRFSNEDIQVSPSYEREKNPFPKEWRMHAFFMDALPILNEPVKHVASNV
                     SDKEAFEDIIQTLKRQSEKVTLLFTGPLTDLAKALQKDSSIVQYIEKLVWMGGTFLPK
                     GNVEEPEHDGSAEWNAYWDPEAVKIVFDSDIEIDMVALESTNQVPLTLDVRQRWANER
                     QYTGIDFLGVSYAAVPPLTHFITNSTYFLWDVLTTAYIGNKDLVHSIEKKVDVISYGP
                     SQGKTFECKDGRKINVINHVDNNAFFDYITALAKKVN"
     gene            4948..5517
                     /locus_tag="NBH32_002240"
     CDS             4948..5517
                     /locus_tag="NBH32_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000208738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HCD1515816.1"
                     /translation="MTTKQLVYTALMTAIIAILGLVPVIPLPFSSVPIVLQNIGIFLA
                     GVILGRKYGTLSVIVFLLLVVAGLPLLSGGRGGIGVFAGPSAGFLLLYPVVAFMIGAI
                     RDRFINEINFWILFVGILVFGVIALDVIGTLIMGMIINIPFTKAISISLAYLPGDILK
                     AIVASLIGTALLNHSQFRQIMGLKKSYLR"
     gene            5574..6344
                     /locus_tag="NBH32_002241"
     CDS             5574..6344
                     /locus_tag="NBH32_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515817.1"
                     /translation="MASISMSDIYCNGTIFENDDEQLIYLTPSFPQRYTSNTWIYKKM
                     PTEERWLKDLERQHQLHTNQGSNHYAFSFPENEQLDNHWMAMFKDMNFELGIMELYAI
                     ESDALANLPRNADVEIAIVDESHIDAYLKVAYQFSLPFGKDYADAHEKMVREHYQKDV
                     IKRLVAYLNNEPIGVVDVIESENYIELDGFGVLEQFRHQGIGSTMQSLVGEYAISRDH
                     KPVILVADGEDTAKDMYAKQGYVYQSFCYQILKEDIGN"
     gene            complement(6521..7318)
                     /gene="fdhD"
                     /locus_tag="NBH32_002242"
     CDS             complement(6521..7318)
                     /gene="fdhD"
                     /locus_tag="NBH32_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCD1515818.1"
                     /translation="MNKDVSLGQPIVRYEDGKLFNKTDQYVTEFPLTIMVNGEEFATV
                     ICSPTNLEELVIGFLASEGAILKRDELKSVLIDDSKGFAHVELNKDLGDRFQYSTKRM
                     IASCCGKSREFYFQNDAAIAKTSMSKITLTPIQIINMMTRLQSASHIYQETGGLHNAA
                     ISDGLTFFVHRQDIGRHNALDKLYGFCIQRHITVRDKVLIFSGRISSEILIKAAKIGV
                     GVILSKSAPTTLAVTLANDLNITSVGFIRNGGFNIYSHPERIIDSEQ"
BASE COUNT         2685 a          980 c         1454 g         2479 t
ORIGIN      
        1 ttaggattta gaaaatagag tttttaatat aactttttgt aaaatggtag tatattaatg
       61 agtaaacatt caagaaaaag ggtgggcatt ttgaaaatga tagatgtact tttgaaaaat
      121 atttctcagg ttgtgttaat tagtaataaa tggacaggat tatttatctt aataggatta
      181 tttgttgctg attggacaat tggattagcg gctattgtag gtagcatcat cgcctatact
      241 tttgcgcgtt ttataaatta tagtgaagca gagattaatg atgggttagc cggatttaat
      301 ccagtgttaa ctgccattgc gttaacaatc tttttagata agtcaggatt agatattgtt
      361 ataacaatga tagcaacttt attaacgtta ccagtcgctg ctgcagtgag agaagtttta
      421 agaccatata aagttccgat gctgacgatg ccttttgtca ttgtgacttg gtttacaatt
      481 ttactatcag gacaggttaa atttgtagat acatctttaa agttaatgcc tcaaaacatt
      541 gaaacggtta attttagcaa caatgataga atacatttca ttcagtcatt atttgaagga
      601 ttcagtcaag tatttatcga agcgagtgta attggtggcg tatgtatttt aatcggcata
      661 ttgatagcat caagaaaagc agcactctta gctgttatag ctagtttgtt aagctttatc
      721 attgtagctc tattaggtgg taattatgat gatattaatc aggggttatt cggttataac
      781 tttgtattaa tggcaatcgc actaggattt acatttaaaa cagagattaa tccttatatt
      841 tcgacttttt taggtgtgtt attaacagta gtggtgcaac taggtacaac aacattgctt
      901 gaaccgtttg gcttacctgc attaacattg ccatttatta ttgtgacttg gattttatta
      961 tttgctggta ttaaacatga caaagtagat gcttgaaagt taaatcaaac ctagtattgt
     1021 ttgaatatca ccttaaacca tacagcgagt tgtatagttt aaggtgtatt tttatggaga
     1081 aaattaattg gatacttaat cgaattatca actttctttt aattgttcac agcacgttcc
     1141 tttttatttt taaagtcgag tgttaaacta ggaataaata atttatattg tgtgttgtgt
     1201 ggggtgacta atatgaatga tatggataat tcctttttaa taacaacgga aattcaaaga
     1261 aaatggattg aaaaattcaa agtaattgga gatacattta aggctaaagc tgaatataat
     1321 gatcaacata gccaatttcc atataaaaat attgaatggt taattaaaga aggttatgga
     1381 aaattaacgt taccaaaaga atatggtggt gaaggtgcga ccatagaaga catggttatt
     1441 ttgcaatcat ttttaggcga acttgatggt gccacagcat tatctattgg ttggcatgtg
     1501 agtgtcgtag gacaaattta tgaacagaaa ttatggtctc aagatatgtt ggagcaattt
     1561 gctgttgaaa ttaataatgg tgcattagtt aatagagcag ttagtgaagc tgaaatgggt
     1621 agtccaacaa gagggggaag accaagtaca catgctgtta aagctgatga cgggtatatt
     1681 ttaaatggtg tgaagacata tacatcaatg agtaaagcac taacacatat tattgttgct
     1741 gcttatatag aagaattaga aagtgttggt tttttcttag tggacaggaa tttgcctggt
     1801 gttgaaattg ccgataattg ggacgtatta ggtatgcgag cgacagaaag tcatgattta
     1861 atattaaatg atgtaaaagt accattaaaa catttagtgg aaacagagaa aagtaaagcg
     1921 ccaaatggtt ggatattgca tataccaagc tgttatttag gtatagcaca agctgctaga
     1981 aattatgctg tagactttgc aatccaacat agccctaata gtattgaagg aacgattgca
     2041 acattgccaa ctgtgcaaca aaatttaggg aaaatggaaa cgctgttgtt atgtgcgaga
     2101 caatttttat ggagtacagc aaaaggctat caacaatata cagacgaaag tcagattaga
     2161 aatgcgacga gtgcgagtaa agtaatggta atgaaccaag gccttgaagt gattgattta
     2221 gcgatgcgca ttgttggtgc taaaagctta gaaatgaatc gaccattaca acgttactat
     2281 cgtgacatgc gtgcaggatt acacaatcca cctatggaag atgcagcata tacaaatatt
     2341 gctaagagta ttacagatac attttaaatt taaaaaggta aatcaatcgt tgaatggcat
     2401 aaagagatga aatttgacgt tcggatttac cttttttgtg ggaatttata ggtatattaa
     2461 gatattttgc taaatttttt gcgaaaataa aaaaataaat aacactagct attgtaaatg
     2521 tatatgcaat cttgtataat tttggaatga ttgataatca ttttcaatag gaggaaatta
     2581 tgaaaaaact attattacca ttaataatta tgttattagt gttagctgcg tgtgggaacc
     2641 aaggagaaaa aaataacaaa gctgaaacta aatcttataa aatggacgat ggcaaaacgg
     2701 tagatattcc gaaagaccct aaacgcattg cagtagttgc gccaacatat gctggtggac
     2761 ttaaaaaatt aggtgcaaac attgtagctg taaatcaaca agtcgatcaa agcaaagtat
     2821 taaaagataa atttaaaggt gttacaaaaa ttggtgatgg cgatgtagaa aaagttgcta
     2881 aagaaaagcc agatttaatt attgtatact ctactgacaa agacattaaa aaatatcaaa
     2941 aagtagcacc aacagtagtt gttgactata ataagcataa atatttagaa caacaagaaa
     3001 tgttagggaa aattgttggt aaagaagata aagtaaaagc ttggaagaaa gattgggaag
     3061 aaacaactgc taaagacggt aaagaaatta aaaaagcaat tggacaagat gcaacagtgt
     3121 cattgtttga tgaatttgat aaaaaattat acacttacgg cgataactgg ggtcgtggtg
     3181 gagaagtatt atatcaagca tttggtttaa aaatgcaacc agaacaacaa aagttaactg
     3241 caaaagcagg ttgggctgaa gtgaaacaag aagaaattga aaaatatgct ggtgattaca
     3301 ttgtgagtac aagtgaaggt aaacctacac caggatatga atcaacaaac atgtggaaga
     3361 atttgaaagc tactaaagaa ggacatattg ttaaagttga tgctggtaca tactggtaca
     3421 acgatcctta tacattagat ttcatgcgta aagatttaaa agaaaaatta attaaagctg
     3481 caaaataatt caactatata agttagtgaa atgagagtct gaaacatatc aatcttttga
     3541 tattgtatta ggctcttgtt tttatagcta gaaagttaga tatttgtatt ttttaaaata
     3601 ataagtgccg ttgttatcat tcaatttaat taatgataga ttagtattat tacagctaaa
     3661 gtagtatacc tgagaaatag ctcaatgtat ctcttcatta ataagttata tcataattat
     3721 tttagtgcat actttatgga aggcatatca gggaatgatt atcaattaaa gaagaggttt
     3781 aaaaggatta caacagaatg ttatgatttt gtagaaagat atataacaac gttttataaa
     3841 aacataatat tgttaatgga aaatgaaatg taagggggat ttcgagtgac taagaaagtt
     3901 tattttaacc atgatggtgg tgtagatgat ttagtatctc tatttttatt attacaaatg
     3961 gaaaacgttc aattgatagg ggtcagtaca attggtgctg attgttattt agagccatct
     4021 ttgagcgcat cagtaaaaat tattaaccgt ttttcaaatg aagatattca agtttcccca
     4081 tcatatgaac gagagaaaaa tccatttcct aaagaatggc gtatgcatgc cttttttatg
     4141 gacgcattgc caattttaaa tgagccagtc aaacacgttg cttcaaatgt gagcgacaaa
     4201 gaagcctttg aagacattat tcaaacttta aagagacaat cagaaaaagt aacattatta
     4261 tttacaggcc cgcttacaga tttagcaaaa gcactacaaa aagattcatc tatcgttcag
     4321 tatatagaaa aattagtttg gatgggtggt acctttttac caaaaggaaa tgttgaagaa
     4381 cctgagcatg atggttctgc agaatggaat gcatattggg atccagaagc ggttaaaatt
     4441 gtttttgata gcgatataga gattgatatg gttgctttag aaagtacgaa tcaagtgccg
     4501 ctaacgttag atgttagaca aagatgggcg aatgaacgcc aatatacggg cattgatttt
     4561 ttaggagtaa gttatgcagc ggtaccacca ttaacacact ttataacaaa ttctacttac
     4621 tttttatggg atgttttaac gactgcttat attggtaaca aggacttagt tcattcaatt
     4681 gagaaaaaag tggatgtaat aagttatgga ccaagtcaag gtaagacatt tgagtgtaaa
     4741 gatgggcgca aaattaatgt cataaatcat gtagataaca acgcattttt tgattatata
     4801 actgcacttg ctaaaaaagt aaattaacag ctgtgtagaa taattaagat tttaatttat
     4861 atagaacagc ttattgtaaa cttttcgttt cttaaagttt acaatggtgc tataataatg
     4921 gtcatgaaat acgaaaggaa gtaaaaaatg acaacaaaac agttagtata tacagcttta
     4981 atgacagcga ttatcgctat tttaggattg gtaccagtaa ttccactacc attttcttca
     5041 gtaccaattg tacttcaaaa cattggtatt ttcttagcag gtgtgatttt aggacgtaaa
     5101 tatggcacat taagtgttat cgtcttttta ttattagtag ttgctggctt gccactttta
     5161 tcaggcggtc gcggtggcat tggtgtattc gcaggtcctt cagcaggatt tttactatta
     5221 tatccagttg tagcattcat gattggtgcg attcgtgata gattcatcaa tgaaattaat
     5281 ttctggattt tattcgtggg tattttagtt tttggtgtta tagcattaga tgttattggt
     5341 acattgatta tgggcatgat tattaacata ccatttacga aagctatttc aatttcatta
     5401 gcttatttgc ctggtgatat attaaaagca attgtagcaa gtttgattgg tacagcttta
     5461 cttaatcact cgcagtttcg tcaaattatg ggattaaaaa aatcatattt aagatagtaa
     5521 agtaattgaa taagttgctt tgaaatttat aaaagtgaaa ggagtaggtg tcaatggcta
     5581 gtataagtat gtcagatata tattgtaacg gcactatatt tgaaaatgac gatgagcagt
     5641 tgatttattt aacaccttct tttccacaac gatatacaag caacacatgg atatataaaa
     5701 agatgcctac cgaagagcga tggctgaaag acttagaacg tcaacatcaa ttacatacaa
     5761 atcaaggttc aaatcattat gcgtttagtt tcccagaaaa tgaacaactt gataatcatt
     5821 ggatggctat gtttaaagat atgaattttg aactaggtat tatggaattg tatgccatag
     5881 aaagtgatgc ccttgccaat ttgccgcgta acgctgacgt tgaaattgcc atcgttgacg
     5941 agtcgcatat agatgcctat ttaaaagttg catatcaatt tagtttgcca tttggaaaag
     6001 actatgcaga tgcacatgaa aaaatggtaa gggaacatta tcaaaaagat gtgattaaac
     6061 gcttagtagc ttatttaaac aatgaaccta ttggcgttgt agatgtcatt gaaagtgaaa
     6121 attacattga attagatgga tttggtgtat tagaacaatt tcgacaccaa ggaatcggtt
     6181 ctaccatgca gtcattagta ggtgaatatg ctatatctag agatcataaa ccggttatat
     6241 tggtggcaga tggtgaagat acagcaaaag atatgtatgc aaagcaaggt tatgtctatc
     6301 aatcgttttg ttatcaaata ttaaaagaag atattggaaa ttaacgattg atggtaggtt
     6361 tgtattagaa taaatgaaaa atataaaaag cagtaagctg gtttcgagta gaaatcgact
     6421 tactgctttt taagttgtga taagtatatt aaacaatgtt gttttaaggt tgttgattca
     6481 tgtagaatat cgttcattat gacacactat aataaatatg ttattgttca gaatcaatga
     6541 tacgttctgg atgactgtat atattaaagc caccatttcg aataaatcca actgacgtaa
     6601 tgtttaggtc attagctaaa gttacagcaa gcgttgttgg agccgattta gataaaatga
     6661 cgccaacacc aattttcgcg gctttaatta aaatttctga tgaaatacgt ccactgaaaa
     6721 ttaatacttt atctcggaca gtaatatgtc gctgaataca aaatccatat aatttatcta
     6781 gagcgttatg tctaccaatg tcttgtcgat gtacaaaaaa tgtcaaacca tcgcttatag
     6841 cagcattatg caggccacct gtttcttggt aaatatgact tgcactttgt aatcgagtca
     6901 tcatgttaat aatttgtatt ggagttaaag tgattttaga catagatgtt ttagcgatag
     6961 cagcatcatt ttgaaaataa aactcacgac tctttccaca acaagatgca atcattcgtt
     7021 ttgtggaata ttgaaagcga tcgcctaaat ctttattaag ttcaacatgg gcaaatcctt
     7081 tactgtcatc aattagtaca gattttaatt catctcgctt taaaatggca ccttccgaag
     7141 ccagaaatcc aatgactaac tcctcaaggt ttgttggact gcatataaca gtcgcaaatt
     7201 cttcaccatt caccataatt gtaagtggaa attctgtcac atattgatct gtcttattga
     7261 ataattttcc atcttcatat ctaacaattg gttgacctaa agatacatct ttgttcatta
     7321 tctaacccct ttaattagct tgaactttat tttaaagtaa tttgcttaaa attttaacat
     7381 atttgcttaa gtttgaaatt tgattgataa aaattaatag cgagcaatta tttagaattt
     7441 gattggattt agaaaaaggc acactagcag ctcaattaat aaaaaataat ttttagaaag
     7501 tgtgacaatt atttgaatca ttatttaaag agaaaacgct tcttttcaaa aatattgtta
     7561 agtgtataat gaaaatgtaa atatttaata attggggg
//
LOCUS       DAHHOJ010000114         7430 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.114, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000114 DAHHOJ010000000
VERSION     DAHHOJ010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.114"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(637..1083)
                     /gene="perR"
                     /locus_tag="NBH32_002243"
     CDS             complement(637..1083)
                     /gene="perR"
                     /locus_tag="NBH32_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide-responsive transcriptional repressor
                     PerR"
                     /protein_id="HCD1515819.1"
                     /translation="MSVEIESIEHELEESIASLRQAGVRITPQRQAILRYLISSHTHP
                     TADEIYQALSPDFPNISVATIYNNLRVFKDIGIVKELTYGDSSSRFDFNTHNHYHIIC
                     EQCGKIVDFQYPQLNEIERLAQHMTDFDVTHHRMEIYGVCKECQDK"
     gene            complement(1180..2130)
                     /locus_tag="NBH32_002244"
     CDS             complement(1180..2130)
                     /locus_tag="NBH32_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCD1515820.1"
                     /translation="MKVVGLNRMREVETELQQSFSDLDFKFYKKASEIPESDLADLDI
                     LVGYDGGINEAFLRRCPNLKWIAWFATGVNTLPLDYIADHGILVTNGKGVQAKQLSEY
                     ILAFILDDYKKMKLSYDNQRQHIYDSKITGKRLSGQTVLFLGTGAIATRTAKLAKAFN
                     MNLIGLSKSGQNKDEFDEIYTIESLESTLPNADIIINALPETQETIHLLKKKHFELMK
                     DEALFINIGRGSIVKESLLIEVLKSRVIRHAYLDVFENEPLKPNHELYELENVTITAH
                     ITGNDYEAKYDLLDIFKNNLVNFLNKNGLIENEVDAKKGY"
     gene            complement(2136..2591)
                     /gene="bcp"
                     /locus_tag="NBH32_002245"
     CDS             complement(2136..2591)
                     /gene="bcp"
                     /locus_tag="NBH32_002245"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCD1515821.1"
                     /translation="MLQKGEQFPIFKLENQDGTVITNDTLKGKKAIIYFYPRDNTPTC
                     TTEACDFRDNLEMFNDLDVAVYGISGDSKKKHQNFIEKHGLNFDLLVDEDFKLAKETG
                     VYQLKKSFGKESMGIVRTTFIIDEQGKVLDVIEKVKVKTQIEELKNILG"
     gene            2672..3961
                     /locus_tag="NBH32_002246"
     CDS             2672..3961
                     /locus_tag="NBH32_002246"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCD1515822.1"
                     /translation="MNFSESERLQQLSNEYILGGVNSPSRSYKAVGGGAPVVMKEGHG
                     AYLYDVDGNKFIDYLQAYGPIITGHAHPHITKAIQEQAAKGVLFGTPTELEIEFSQKL
                     RDAIPSLEKIRFVNSGTEAVMTTIRVARAYTKRNKIIKFAGSYHGHSDLVLVAAGSGP
                     SQLGSPDSAGVPESVAREVITVPFNDINAYKEAIEFWGDEIAAVLVEPIVGNFGMVMP
                     QPGFLEEVNEISHNNGTLVIYDEVITAFRFHYGAAQDLLGVIPDLTAFGKIVGGGLPI
                     GGYGGRQDIMEQVAPLGPAYQAGTMAGNPLSMKAGIALLEVLEQDGVYEKLDSLGQQL
                     EEGLLKLIEKHNITATINRIYGSLTLYFTDEKVTHYDQVEHSDGEAFGKFFKLMLNQG
                     INLAPSKFEAWFLTTEHTEEDIKQTLKAADYAFSQMK"
     gene            4254..5348
                     /locus_tag="NBH32_002247"
     CDS             4254..5348
                     /locus_tag="NBH32_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014667727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HCD1515823.1"
                     /translation="MRLGARIFKTGIAIILAMSIASLLPDDVGLKALAGVSAVVAMQP
                     SIYRSFKTVSDQALGNIIGAILSVTMVTIFSDNFIIMGVTVIVLIAILFKFNLAHVAT
                     LASVTALIIMGQHTGSFYITAFYRFVLVMIGVISSSLVNFVFLPPKFETKIYYNSLNI
                     SSDIFMWFKLVLNDTTEFNNIKQDSHNLKQRVEKLEKIYDYYSEERPITKKHIHQQNR
                     KKILFREVVQTTRQAYEVLNKLSRYQNDLYLLNNNFLLQIKLDLDSLTAFHEQILASL
                     SKKARYNVTHVDYELDNPQKKDLLSTFQHELINHPYQTEYSFANVMQIVAAIEEYRHH
                     LEHLDRIRISFFTYHRSDADIEIVEEDFDL"
     gene            complement(5438..5530)
                     /locus_tag="NBH32_002248"
     CDS             complement(5438..5530)
                     /locus_tag="NBH32_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515824.1"
                     /translation="MFDFSTILKRKFVTGWWGHNIEKAVRDFLN"
     gene            complement(5539..7275)
                     /locus_tag="NBH32_002249"
                     /pseudo
     CDS             complement(5539..7275)
                     /locus_tag="NBH32_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500500.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SAV1866 family putative multidrug efflux ABC
                     transporter"
BASE COUNT         2455 a         1235 c         1059 g         2681 t
ORIGIN      
        1 tttgtcactt acaagattta attataactc gtaagttttt gtaagtcaag aattattttt
       61 aattatttta tttctaaaaa ataaatttcc taactcaatt aattcaacaa atattatttt
      121 attatgtatt tgttcgaatt tcaatagtca aatttacact ttgttaatat tttaaatcaa
      181 caacttaaaa catttgtttt ttaaatatta gtgccgtttg acgactttta tataatatca
      241 agttattcta gtagagtcaa cataaaacaa cacttatttt tgtttaaaaa ttactgttga
      301 agggattatt ttctattcgt aagtactata tgtatttaaa gtgtaattcc ttacgctttt
      361 tacttcagtt ttacaaacaa acttatcctc actttatgga tagttattta gtgaaccttc
      421 gtttgaatat ttttatgttt atttataaag gatactagtt attagttaat accacttccg
      481 tagttaaaat gttcttttct tctatttaaa tatttttcag aatgagcata aaaaagaagt
      541 taaacaactt tcatgtcgtt taacttctta aatattacac ttacttatat gaagtaagct
      601 aacgcttttt taatttgtca tactaccaaa gttaaattat ttatcttggc attctttaca
      661 aactccataa atttccattc gatgatgtgt tacgtcaaag tcagtcatat gctgagctaa
      721 tctttcaatt tcatttaact gtggatattg aaaatcaaca atcttaccac attgttcaca
      781 tataatatga taatgattat gtgtattaaa gtcgaatcga cttgatgagt ctccatatgt
      841 taattctttt acaattccaa tatctttaaa cactcttaag ttattatata ttgtcgcaac
      901 acttatattt ggaaaatcag gtgaaagtgc ttgataaatt tcatcagctg ttggatgagt
      961 atgtgaagaa attaaataac gtaatattgc ttgtctttga ggtgtaattc ttacgcctgc
     1021 ttgtcgcaat gatgcaattg attcttctag ttcatgttca attgattcta tttcaacact
     1081 catctatatc accatctttc ttatataata ataattatta cttactataa atataactgt
     1141 agtattgcgc gcgtgtcaat atttacatga tgatttcatt taatagcctt ttttagcatc
     1201 aacttcattc tcaattagac cattcttatt gagaaaatta actagattgt ttttaaaaat
     1261 atctaataag tcatactttg cctcataatc attaccagtt atatgcgctg ttatagttac
     1321 attttccaat tcatataatt catgattagg tttcaaaggt tcattttcaa acacatctaa
     1381 atacgcatgt cgaataactc tactttttaa tacttctatt aagagcgatt ctttaactat
     1441 gctacctcgt cctatattta taaaaagtgc ttcatctttc attaattcaa aatgtttttt
     1501 ctttagtaaa tgaatcgttt cttgcgtttc tggtaaagca tttataataa tgtcagcatt
     1561 tggtaatgtg ctttcaagtg attcgatagt atatatctcg tcaaactcat ctttgttctg
     1621 acctgacttg ctcagaccaa ttaaattcat attaaaagcc tttgctaact tcgcagttct
     1681 agtagcaatt gcacctgtac ctaaaaataa aactgtttgt cctgataggc gtttaccagt
     1741 tattttcgaa tcatatatat gttgtcgttg gttatcatat gatagtttca tctttttata
     1801 atcatctaaa atgaaagcta aaatgtattc agataattgt ttagcttgaa caccttttcc
     1861 attagttaca agtatgccgt gatctgcaat ataatctaac ggtaatgtat ttacacccgt
     1921 agcaaaccat gcaatccatt ttaaattcgg gcaacgtcgt aaaaatgcct cattgatacc
     1981 gccatcataa ccaactaata tatctaaatc agccaagtcg ctctcaggta tttctgatgc
     2041 ttttttataa aatttaaaat ctaaatctga aaagctttgt tgtaattcag tttcaacttc
     2101 acgcatacga tttaacccaa caactttcat atatgtcacc ccaaaatgtt tttaagttct
     2161 tctatttgtg ttttaacctt aaccttctcg ataacatcta atactttgcc ttgttcatct
     2221 attataaaag tcgttcttac aatgcccata ctttctttgc caaatgattt tttcaactga
     2281 tatacgccag tttctttagc taatttaaaa tcttcatcta ctaataaatc gaaattcaat
     2341 ccgtgtttct caataaaatt ttggtgtttt ttctttgaat caccgcttat accatatact
     2401 gcaacatcta aatcattgaa catttctaaa ttgtctctaa agtcacaagc ttctgtggta
     2461 caagtaggtg tattatctct aggataaaaa tatataatcg cctttttacc ttttaatgta
     2521 tcatttgtaa tgacagttcc gtcttgattt tctaatttaa atattggaaa ttgttctcct
     2581 ttttgcaaca tataatcacc tttttcttca ttataataat tttatgatac gatattagat
     2641 gaaaatatta aattaaaaga ggttgatata aatgaatttt agtgaaagtg aacgtttaca
     2701 acaactttca aacgaatata ttctaggcgg tgtcaattcc ccttctcgtt cttataaagc
     2761 tgtaggaggc ggtgcacctg ttgttatgaa agaaggacac ggtgcatatt tatatgatgt
     2821 cgatggcaat aaatttattg attaccttca agcatacggt ccaattatta cggggcatgc
     2881 acatcctcat attactaaag caattcaaga acaagcagct aaaggtgttt tatttggtac
     2941 accgactgaa ctagaaattg aattcagcca aaaattacgt gatgcaattc catctcttga
     3001 gaaaattcgc tttgtaaatt ctggaacaga agcagtcatg acaacaattc gtgttgcacg
     3061 tgcatatact aaaagaaata aaattataaa atttgctgga tcttatcatg gccattctga
     3121 tttagtattg gttgcagcag gtagtggccc atctcagctc ggttctccag actcagctgg
     3181 tgttccagaa agcgtcgcac gtgaagtcat tactgtacct ttcaatgata ttaacgcata
     3241 taaagaagca attgaatttt ggggtgatga aattgccgca gtattagtag aaccaattgt
     3301 tggtaacttt ggaatggtaa tgcctcaacc tggattttta gaagaggtta atgaaatttc
     3361 gcataacaat gggacactag tgatttatga tgaagtaatt actgcattcc gtttccatta
     3421 cggtgccgct caagatttat taggtgttat ccctgattta actgcatttg gtaaaattgt
     3481 tggcggtggt ttaccaattg gaggctatgg tggacgtcaa gatattatgg aacaagtagc
     3541 acctctagga cctgcatatc aagctggtac aatggctggt aacccattat ctatgaaagc
     3601 aggtattgca ttactcgaag tactagaaca agacggtgtt tatgaaaaat tagacagctt
     3661 aggccaacaa ctagaagaag gtttacttaa attaatcgaa aaacataata tcacagctac
     3721 aattaatcgt atttatggat ctttaacatt gtactttaca gatgaaaaag tcacacatta
     3781 tgatcaagtt gaacattctg acggcgaagc gttcggtaaa tttttcaaat taatgttaaa
     3841 tcaaggtatc aatttagcac cttctaagtt tgaagcttgg ttcttaacaa ctgaacatac
     3901 agaagaagat attaaacaaa ctttaaaagc tgcagactat gcttttagtc aaatgaaata
     3961 acttttaagg aagtatattt ttaattgaat taaaataaag caaatgctca catagtagat
     4021 tcattttata tcgttaacta ttaacgttta acttgatatg tcactatgtg agtttttatt
     4081 ttacaaattt atactctttt aatctctgac taaagtttta catcactatc agtattatgt
     4141 atgatttatt ttaacaacta taaataagct tgaattttgt aactagtaag tgtataacag
     4201 taatgaatac gaatattaga tattcaataa acttaaaagg ggcgtcatta tttttgagac
     4261 taggagctcg gatttttaaa actggtatag ccattatttt agctatgtct atcgcttctt
     4321 tactaccgga tgatgttggt ctgaaagcct tagctggtgt cagtgctgtt gttgcaatgc
     4381 aacctagtat ttatcgttca tttaaaacag tttctgatca agcattgggt aacattatag
     4441 gtgccatact atctgttaca atggtaacaa tttttagtga taattttatc attatgggtg
     4501 ttaccgtcat agtacttatt gctattttat ttaaatttaa tttagcacat gttgctactt
     4561 tggcgagtgt aacagcatta atcattatgg gacaacatac cggttctttc tatattaccg
     4621 ccttttacag gtttgtactt gtaatgattg gtgtcataag ttcatctcta gttaactttg
     4681 tgtttctacc acctaaattt gaaacaaaaa tttactataa ttcattaaat atatcctcag
     4741 atatttttat gtggtttaaa ttagtattaa atgatacgac ggagtttaat aatattaaac
     4801 aagatagtca taatttaaaa caacgtgtcg aaaagttaga gaaaatttat gattactata
     4861 gcgaagaacg tcctattaca aaaaaacata ttcaccaaca gaatagaaag aaaatacttt
     4921 tcagagaagt agttcagacg actagacaag cttatgaagt attaaacaaa ttgtcgcgtt
     4981 atcaaaacga cttatattta cttaataaca atttcctatt acaaatcaaa ttagatttag
     5041 attcattaac tgcttttcac gagcaaattt tagctagcct ttctaaaaaa gcacgttata
     5101 atgtgacaca tgttgattat gagttagata atccgcagaa aaaagattta ttgtccacat
     5161 ttcagcatga gttaataaat catccttatc aaacggaata ttcgtttgcc aatgttatgc
     5221 aaattgttgc agccatcgaa gagtatcgac accatttaga gcatttagac cgcataagaa
     5281 ttagcttttt cacatatcat agatctgatg cagacattga aattgttgaa gaagactttg
     5341 atttataagt ttaaagcaat tcctgtaatt aataccacaa tgagataaag taatattgaa
     5401 tctcgaaatt aaattaaaaa aagcagtaag attattttca attaagaaaa tctcttactg
     5461 ctttttctat gttatgaccc caccatcctg ttacaaattt tctcttcaag attgtactga
     5521 agtcaaacaa ctaatacttt ataagttttg aatgctatat aaatgctcgt aagcaccttg
     5581 ttttgacatc aattcacgat gcgtacctgt ttcaacaata tgtccatttt caattacgac
     5641 aattttgtca gcatgtgtaa tagtggacaa gcgatgcgct acgataagtg tcgttcgatc
     5701 tttactcaac acatctaatg cttcttgaat aatggattca ctttctaaat caagtgcact
     5761 tgttgcttca tccaagataa gaattggcgg attatttaaa aatattctag caatcgataa
     5821 tctttgtttt tgaccacctg ataatttaac acctcgttca cctacttcag tgtcatatcc
     5881 ctgtggcaag ttcataataa agtcatgtgc attagccatt ttcgccgctt caactacttc
     5941 ttcatctgtt gctgttggac gaccaagtaa aatattttcc ttaactgtat cagagaataa
     6001 aatattatcc tgttgcacca atcctatttg atttcttaaa cttcccgtta aaaaatcttt
     6061 aatgttgtga ccatctatta aaatttgccc agaagttaca tcgtaaaatc tcggtattaa
     6121 gttaattaat gttgatttac caccaccact catacctacg aaagcaactg tttctccttt
     6181 ttcaatactc aaattaatat cttttaaaat tggagcttcg ttatcgttat attaaaaact
     6241 aacatgatca atatcaatac gaccttgttt aatttcaata ggttgagcac caacaccatt
     6301 tttaatgtca taatcttcgt caattaattg gaatacacgg tccattgaag caaaactttg
     6361 cgttaaagtt gtaaatgatg cgactaaacg acgtaaaggc ccgaacaata actctaagta
     6421 tccaacaaat gctgcaagtg tacctactgt gattgatcca gaaatagcaa gatatgcacc
     6481 aacaccgatg acaattattg gtccaatatc tgtaactgta ttaattgcgg caaaggaata
     6541 ggcattccat cttgtatgtt tcaaagcacg tgttaagaaa ttagtgttct ttttatcaaa
     6601 gtttttcgct tcattgtctt caatcgcaaa acttttaacg actgaaatac cttgaacacg
     6661 ttcatgcaag aatccttgaa cctcagctaa tgcttgcgat ctctcacgcg tcaattttct
     6721 taatcttcca aagaaaacgt acaccgttaa aatgtaaaat gggaagataa acagtgctgc
     6781 taaagtcaat ttcacatcta aaaagaacat tatggatagt gcaataataa ttgttataca
     6841 atctaaccaa atattcatta acccggttaa aatgaaatct tttgtttgtt caacatcatt
     6901 aatcactcta gatattactt gacctacttg attattagca taaaatctcg cacttaaagc
     6961 ttgtaaatgg ttgtataact ttttacgtat atcatacaat attttattac ttgtccattg
     7021 cgccaaatat tgacgtataa attcaattgg tggtctcact attacaaaaa taaataatgc
     7081 gataccaatg gcaatagtta aatgatgaac tttttcatca gtcgttagtg cgtggttatt
     7141 aatcacgcca tctattgcat atttaattaa aagtggtata agcattggta taccaaactt
     7201 aattattcca acaataatcg ttgcaaaaat acgatatttg tatggcttaa caaattgcaa
     7261 atatcgttta atcatacaat ccccccctaa tctattgccc tatcctattc ataagcataa
     7321 aaatgaatag aggttggata cataatttgt agatgtaaat tcttcttaca atttacattt
     7381 ttaaaattaa gatatctcaa cctctgtcat attcatgtga ttaaatacgt
//
LOCUS       DAHHOJ010000115         7194 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.115, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000115 DAHHOJ010000000
VERSION     DAHHOJ010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7194
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.115"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            73..501
                     /locus_tag="NBH32_002250"
     CDS             73..501
                     /locus_tag="NBH32_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515825.1"
                     /translation="MTRSALKPFKNKRVMVTGRIQRVLFKNYLDRHSTFKPNVRILLK
                     DVFVSGVSIDHLWLYETNKYYALAMELIHQRVKFSANVVPYYKINRNNNLFVQDYGIK
                     RKGRLITEEAYNQNNQYQDKIYEKLPDIDFRLEDFYSKEN"
     gene            complement(710..1216)
                     /locus_tag="NBH32_002251"
     CDS             complement(710..1216)
                     /locus_tag="NBH32_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515826.1"
                     /translation="MAHIIRRVSIKDVENFISMLAKIYDESPYMFYTPGEYDPSVTSA
                     SKQLEEYITSPHKVIFVAESDEQLVGFAFVNTTPFQRIKHVAKIDLGVKKLYQHRGIG
                     QALLDAIMAWCLNNQIHRIEANVPLNNQPALELFKSADFQIEGVLKDKLFIDGKYYDD
                     YMMAKILN"
     gene            1329..2252
                     /gene="graX"
                     /locus_tag="NBH32_002252"
                     /gene_synonym="apsX"
     CDS             1329..2252
                     /gene="graX"
                     /locus_tag="NBH32_002252"
                     /gene_synonym="apsX"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="auxiliary protein GraX/ApsX"
                     /protein_id="HCD1515827.1"
                     /translation="MKPKVLLAGGTGYIGKYLSEVIENDAELFAISKYPDNKKTDDVE
                     MTWIQCDIFHYEQVVAAMNQVDIAVFFIDPTKNSAKITQSSARDLTLIAADNFGRAAA
                     INQVKKVIYIPGSRYDNETIERLGAYGTTVETTNLVFKRSLVNVELQVSKYDDVRSTM
                     KVVLPKGWTLKNVVNHFIAWIGYTKGTFVKTEKSHDQFKIYIKNKVRPLAVFKIEETA
                     DGIITLILLSGSLVKKYTVNQGKLEFRLIKESAVVYIHLYDYIPRLFWPIYYFIQAPM
                     QKMMIHGFEVDCRIKDFQSRLKSGENMKYTK"
     gene            2268..2942
                     /gene="graR"
                     /locus_tag="NBH32_002253"
                     /gene_synonym="apsR"
     CDS             2268..2942
                     /gene="graR"
                     /locus_tag="NBH32_002253"
                     /gene_synonym="apsR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor
                     GraR/ApsR"
                     /protein_id="HCD1515828.1"
                     /translation="MQILLVEDDNTLFQELKKELEQWDFNVAGIEDFGKVMDTFESFN
                     PEIVILDVQLPKYDGFYWCRKMREVSNVPILFLSSRDNPMDQVMSMELGADDYMQKPF
                     YTNVLIAKLQAIYRRVYEFTAEEKRTLTWQDAVVDLSKDSIQKGDQTIFLSKTEMIIL
                     EILITKKNQIVSRDTIITALWDDEAFVSDNTLTVNVNRLRKKLSEISMDSAIETKVGK
                     GYMAHE"
     gene            2935..3975
                     /gene="graS"
                     /locus_tag="NBH32_002254"
                     /gene_synonym="apsS"
     CDS             2935..3975
                     /gene="graS"
                     /locus_tag="NBH32_002254"
                     /gene_synonym="apsS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase GraS/ApsS"
                     /protein_id="HCD1515829.1"
                     /translation="MNNLKWVAYFLKSRMNWIFWILFLNLLMLGISLIDYDFPIDSLF
                     YIVSLNLSLTMIFLILTYFKEVKLYKHFDKDKEIEEIKHKDLAETPFQRHTVDYLYRQ
                     ILAHKDKVVDQQLQLNMHEQTITEFVHDIKTPVTAMKLLIDQEENQERKQALLFEWSR
                     INSMLDTQLYITRLESQRKDMYFDYVSLKRMVIDEIQLTRHISQVKGIGFDVDFKVDN
                     HVYTDIKWCRMIIRQILSNALKYSENYNVDISTELIDQHVALIIKDHGRGISKKDMPR
                     IFERGFTSTANRNETTSSGMGLYLVDSVKDQLGIQLQVTSTIGKGTTVKLIFPLQNEI
                     VERMSEVTNLSF"
     gene            4122..4883
                     /gene="vraF"
                     /locus_tag="NBH32_002255"
     CDS             4122..4883
                     /gene="vraF"
                     /locus_tag="NBH32_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000153733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein VraF"
                     /protein_id="HCD1515830.1"
                     /translation="MTILEVKQLTKIYGNKKMAQEVLRDINLSVEEGEFIAIMGPSGS
                     GKTTLLNVLSSIDYISQGSITLKGHKLEKLSNKALSDIRKHDIGFIFQEYNLLHTLTV
                     KENIMLPLTVQKLDKDTMLDRYEKVAEALNILDISDKYPSELSGGQRQRTSAARAFIT
                     LPSIIFADEPTGALDSKSTQDLLKRLMKMNEEIKTTIIMVTHDPVAASYANRVVMLKD
                     GQIFTELYQGDDDKHTFFKEIIRVQSVLGGINYEF"
     gene            4873..6762
                     /locus_tag="NBH32_002256"
     CDS             4873..6762
                     /locus_tag="NBH32_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515831.1"
                     /translation="MSFNEIIFKNFRQNLSHYAIYLFSLITSVVLFFSFVALKYTHSI
                     NITETYPIIKEGSQVGSYFLFFIIIAFLLYANLLFLKRRSSELALYQTLGLSKYNIIY
                     ILMFEQLLIFSITAILGITIGIFGSKILLMIVFTLLGIKEKVPIIFSFKAVFETLLLI
                     GVAYFLTAAQNLILVLKQSISELSKHNQAKQMNHNKITFEEVILGILGIILIISGYYY
                     SLRIVHYYDAFGVLMFILFATVIGAYLFFKSSVSLVFKIIKRFKKGVISVNDVMFSSS
                     IMYRIQKNAFSLTVMAIISAITLSVLCFAAISRATLSSEIKYTSPHDVTIKDQQKANQ
                     LASELNNQKIPHFYNYKEVIHTKLYKDDLFDIKAKEPYNVTITSDKYIPNTDLKRGQA
                     DLFVAEGSIKDLVKHKKHGKAVIGTKKHHVDIKLRKDINKIYFMTDVDLGGPTFVLND
                     QDYQEIRKYTKPKHIVSQFGFDLKHKKDMLALEKATNKVDKSIETRSEAISSISSLTG
                     ILLFVTSFLGISFLIAVCCIIYIKQIDETEDELENYSILRKLGFTQKDMAKGLKFKIA
                     FNFGLPLVIALSHAYFTSLAYMKLMGTTNQIPIFIVMGLYICMYAIFAITAYNHSKRT
                     IRHSI"
BASE COUNT         2645 a          913 c         1257 g         2379 t
ORIGIN      
        1 gaaaagaatt caaaagaaaa attaaaatat agattgagca cgaagtgatt tgaaataagg
       61 ttgtgaaagg gaatgacaag gtcagcatta aaaccattta aaaataaacg cgttatggtt
      121 actggacgta tacaacgtgt tttgtttaaa aattatttag atagacatag cacatttaag
      181 ccgaatgtaa gaatattatt aaaagatgta tttgtttcag gtgtatcaat agatcattta
      241 tggttatatg agacaaataa atactatgca ttggcaatgg aacttattca tcaacgagta
      301 aaatttagtg cgaatgttgt accgtattac aaaataaata gaaataataa tttattcgta
      361 caagattatg gaattaagcg taaaggtagg ttaattactg aagaagctta caatcaaaac
      421 aatcagtatc aggataagat atatgaaaaa ttaccggata tagattttag actcgaagat
      481 ttttatagta aggaaaacta aacaacatat ataaagcacc actaaccgta gttgatagtt
      541 aatcaattac acattagtgg tgttttattt tgaatttaaa tttgataaat gaataagagt
      601 atacgcattc atttaaaatt agatgacatt gataaggatg agtaatgtat taggtaacca
      661 ttaaattcgt tgttatgcga ttctgataca agattatgat aaaatagctt taattaagaa
      721 ttttagccat catatagtca tcgtaatatt taccatcgat aaataactta tcttttaaaa
      781 cgccttcgat ttgaaaatcg gcacttttaa aaagctcgag ggcaggttgg ttattgagtg
      841 gtacatttgc ttcaattcgg tgtatttgat tgtttaaaca ccaagccata atggcatcaa
      901 gaagtgcttg gccaattcca cgatgttgat ataatttctt tacacctaaa tcaattttag
      961 caacatgttt aatgcgttga aatggtgtcg tattaacaaa ggcaaagcca acgagttgtt
     1021 catcactttc agcaacaaag atgactttat gtggagaagt gatatattct tctaattgtt
     1081 tactagccga tgtgacgcta ggatcatatt ctcctggtgt gtaaaacata tacggagatt
     1141 cgtcgtatat tttcgctaac attgaaatga aattttctac atctttgata ctaactctac
     1201 gtataatatg ggccataaaa agcctccagt attttgaaat tttaaaacat ttgctactat
     1261 tataatatat atttaaccaa aaggtagagt aatatgtttt ttggttacag tttaaaggag
     1321 tattttagat gaaacctaaa gttttattag caggtggaac agggtatatt ggtaagtatt
     1381 taagtgaagt gattgaaaat gatgcggaac tttttgctat atcaaaatat ccagacaata
     1441 aaaaaacaga tgatgttgaa atgacttgga ttcagtgtga tatatttcat tacgaacagg
     1501 ttgttgcagc aatgaatcaa gtagatattg ctgtattctt tatcgaccca acaaagaact
     1561 ctgccaaaat aacacaatca tcagcaagag atttaacatt aatcgcagca gataattttg
     1621 gtcgagcagc tgctatcaat caagtaaaaa aagtaatcta catacctggg agtcgttatg
     1681 ataatgaaac aattgaacgc ctaggtgcat atggtacaac tgtagaaaca acaaatttag
     1741 tttttaaacg ttctttagtt aatgtagaat tacaagtttc aaagtatgat gatgttagat
     1801 caacgatgaa ggtagtttta ccaaagggat ggacattaaa gaacgttgta aaccatttta
     1861 ttgcatggat aggttatact aaaggaactt ttgtgaaaac agaaaaatca catgatcaat
     1921 ttaagatata tattaagaat aaggtgcgac cgctcgcagt atttaaaata gaagagacag
     1981 ctgatggaat aataacttta attttattga gtggaagttt agtgaaaaaa tatacagtta
     2041 atcaagggaa gttagaattt agattaatca aagagtcggc agtcgtttat atacatctat
     2101 acgattatat ccctcgatta ttttggccga tttattactt tatacaagca ccaatgcaaa
     2161 aaatgatgat tcatggcttt gaagttgact gccggattaa agattttcaa agtcgattaa
     2221 aatcaggaga aaatatgaaa tatactaaat gatattgggt gatatggatg caaatactac
     2281 tagtagaaga tgacaatact ttgtttcaag aattgaaaaa agaattagaa caatgggatt
     2341 ttaatgttgc tggtattgaa gatttcggca aagtaatgga tacatttgaa agttttaatc
     2401 ctgaaattgt tatattggat gttcaattac ctaaatatga tgggttttat tggtgcagaa
     2461 aaatgagaga agtttccaat gtgccaatat tattcttatc atctcgtgac aacccgatgg
     2521 atcaagtgat gagtatggaa cttggcgcag atgattatat gcaaaagcct ttttatacaa
     2581 atgtattaat tgctaaatta caagcgattt atcgtcgtgt ctatgagttt acagctgaag
     2641 aaaaacgtac attgacttgg caagatgctg tcgttgatct atcaaaagac agtatacaaa
     2701 aaggtgatca gacgattttc ctgtctaaaa cagaaatgat tattttagaa attcttatta
     2761 ccaaaaaaaa tcaaatcgtt tcgagagata caattatcac tgcattatgg gatgatgaag
     2821 catttgttag tgataatacg ttaacagtaa atgtgaatcg tttacgaaaa aaattatctg
     2881 aaattagtat ggatagtgca atcgaaacaa aagtaggaaa aggatatatg gctcatgaat
     2941 aatttgaaat gggtagctta ttttttgaaa tctcgcatga actggatatt ttggatattg
     3001 tttttaaact tgcttatgtt aggcattagt cttatcgatt atgattttcc aatagacagt
     3061 ttattttata ttgtttcttt gaatttaagt ttaacaatga tttttcttat attgacatat
     3121 tttaaagaag taaaattata taagcatttt gacaaagata aagaaataga agaaattaaa
     3181 cataaagatt tagcggaaac gccatttcaa cgtcatacag ttgattattt atatcgtcaa
     3241 attttagcgc ataaagataa agtggtcgac caacagttac aattgaacat gcatgaacaa
     3301 accattacag aatttgtgca cgacataaaa acacctgtga cagccatgaa attattaatt
     3361 gaccaagaag agaatcaaga aagaaaacaa gcgctattat ttgaatggtc acgtattaac
     3421 tcgatgttag atacgcaatt gtatattacg agattagaat ctcaacgcaa agatatgtat
     3481 tttgattacg tgtcacttaa acgcatggtc attgatgaaa tacaattaac aagacatatt
     3541 agtcaggtta aaggtattgg ttttgatgtt gactttaaag tggataatca tgtgtacacg
     3601 gatataaaat ggtgtcgtat gattattaga cagattttat caaatgcttt gaaatatagt
     3661 gagaattata atgttgatat ttcaacggaa ttaattgatc aacatgttgc gttaatcatc
     3721 aaggatcacg gaagaggtat tagtaaaaaa gatatgcctc gaatatttga acgtgggttt
     3781 acgtcaacgg ctaacagaaa tgaaacgaca tcctccggca tgggtctata cttagtggat
     3841 agtgtgaaag atcaattagg aatacaatta caagtcactt cgactattgg aaaaggaaca
     3901 acggttaaat tgatcttccc attacaaaat gaaattgttg agcgcatgtc agaagtgaca
     3961 aatctgtcat tttaaacatg cgttttgtta cttagaattg atacatcatg accacttcaa
     4021 cgatataatc aaatagatgt taattaaata caaaattgaa ggtaagcgtg ccaaattgaa
     4081 tataaaggaa atgaagttaa gataaatata ggagtgttaa agtgacaata ttagaagtaa
     4141 aacaattaac aaaaatatat ggcaataaga aaatggcaca agaagttctg cgagatatca
     4201 atctgtctgt tgaagaaggt gaatttattg ctattatggg accttcagga tctggtaaaa
     4261 cgaccttatt aaatgttttg agttcaattg attatatttc gcaaggttcc atcacactaa
     4321 aaggacacaa acttgaaaag ctttcaaaca aagcattatc tgatattcga aagcatgata
     4381 ttggttttat ttttcaagaa tataatttac tgcatacatt gactgttaaa gaaaatatca
     4441 tgttaccatt aacggttcag aaattagata aggacacaat gttagatcgt tatgaaaaag
     4501 tggcagaagc attaaatata ttagacatca gtgataaata tccatccgaa ttgtctggtg
     4561 gtcaaagaca acgaacatca gctgccagag catttataac attgccttct attatatttg
     4621 ccgatgaacc tacaggtgca ctcgattcga aaagtacaca agatttatta aagcgattaa
     4681 tgaaaatgaa tgaagagatt aagacgacga ttattatggt gacacatgat ccggttgcag
     4741 ctagctatgc aaatcgagta gtgatgctta aagatgggca aatattcacg gagttatatc
     4801 aaggtgatga tgacaaacat actttcttta aagaaatcat acgtgtacaa agtgttttag
     4861 gtggcattaa ttatgagttt taatgagatt atatttaaaa atttccgcca aaatttatca
     4921 cattatgcca tttatctttt ttcgctaatt acaagtgtag ttttgttttt tagcttcgta
     4981 gcactaaaat atactcatag cattaacata actgagacat atcccattat taaagaagga
     5041 tcgcaagtag gtagttactt tctatttttt atcattattg cctttttgct atatgccaat
     5101 ttactatttc ttaaacgtcg tagttctgaa cttgcattat atcaaacatt aggtttatcc
     5161 aaatacaaca ttatatatat attgatgttt gaacaattat taatattttc aataacagct
     5221 atattgggca ttacgattgg tatctttggt tctaaaattt tattaatgat agtgtttact
     5281 ttattaggta tcaaagaaaa ggttccaatt attttcagct ttaaagcagt gtttgagacg
     5341 ctattattaa ttggcgttgc ttatttccta actgcagctc aaaatcttat cttagtattg
     5401 aaacaatcta tttcagaact atcaaagcat aatcaggcta agcaaatgaa tcataataaa
     5461 attacatttg aagaagttat actaggaatt ctaggtatta ttttaatcat cagtgggtat
     5521 tactactctt tacgcattgt acattactat gatgcatttg gcgtccttat gtttatattg
     5581 tttgcgacag taattggggc gtatttattt tttaaaagtt cagtatccct ggtatttaaa
     5641 attattaaaa ggtttaaaaa aggtgtaata agtgtaaatg atgtcatgtt ttcttcatct
     5701 attatgtatc gtattcagaa aaatgcattt tcacttactg ttatggcaat tatttcagct
     5761 attactttat cagtactttg ttttgctgct atcagtagag caactttatc aagtgaaatt
     5821 aagtacacgt cacctcatga tgttacgatt aaagatcaac agaaggcgaa tcagttagca
     5881 agtgaattga acaaccaaaa aattcctcat ttttataatt ataaagaagt gattcataca
     5941 aaactttata aggatgactt atttgatata aaagcaaagg aaccatataa tgtaacaatt
     6001 actagtgata aatatattcc taatacggat ttgaaacgtg gtcaggctga tttatttgta
     6061 gcggaaggct ctattaaaga tttagtgaag cataagaaac atggtaaggc agtgatagga
     6121 acgaaaaagc accatgtgga tattaaatta cgtaaagata tcaataaaat ttactttatg
     6181 actgatgttg acttaggtgg accgacattt gtgttaaacg atcaagatta tcaagagata
     6241 agaaagtata ctaagccaaa acacattgtt tctcaatttg gatttgattt aaaacataaa
     6301 aaagatatgt tagctttgga aaaagcaaca aataaagttg acaaatctat tgaaacaaga
     6361 agtgaagcca taagttcgat atcgagttta acgggaatat tgttatttgt aacctcattt
     6421 ttaggaatta gtttcctaat tgccgtatgt tgtatcatat acattaaaca aatagatgaa
     6481 actgaagacg agttagaaaa ttatagtatt cttagaaaac ttggatttac acaaaaagat
     6541 atggcaaaag gattaaaatt taaaattgcg tttaattttg gattgccatt agtcattgca
     6601 ctttcacatg catattttac atcattagca tatatgaaat tgatggggac aacgaatcaa
     6661 ataccgatat ttatagtaat gggattatat atttgtatgt atgccatttt tgcaataaca
     6721 gcttataatc attctaagcg tacaatcaga cactcaattt aatatatata gcagctttca
     6781 gtaggctagt gaattaagat tcatgagcta tactgaaagc ttttattttt ggatagttat
     6841 aaatccgggc acgacagttt taatgtatat gtttcaactt tgtaaattgt attaaatgtg
     6901 agaaaataga gccataggca ttgaaagaaa tatgaaataa gaatagttag aagaaagata
     6961 agtgagtttt aatatgtaaa agatatgtaa taaatattgc atcgttgttt aatttccaac
     7021 tatgtccatt tacaattatt agagttttgt agataacaaa ttgcctatat atcaagtttt
     7081 tcaaaacaaa tgcctaccac tatttaaatt ttatgtaaaa gttaagtttc ggaattgtta
     7141 aaaatttgta aattgaattg tgggattgta aattttgtgc tattttcata taag
//
LOCUS       DAHHOJ010000116         7187 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.116, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000116 DAHHOJ010000000
VERSION     DAHHOJ010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7187
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.116"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            13..145
                     /locus_tag="NBH32_002257"
                     /pseudo
     CDS             13..145
                     /locus_tag="NBH32_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499947.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            407..1066
                     /gene="arlR"
                     /locus_tag="NBH32_002258"
     CDS             407..1066
                     /gene="arlR"
                     /locus_tag="NBH32_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor ArlR"
                     /protein_id="HCD1515832.1"
                     /translation="MTQILIVEDEQNLARFLELELTHENYNVDTEYDGQDGLDKALSH
                     YYDLIILDLMLPSINGLEICRKIRQQQSTPIIIITAKSDTYDKVAGLDYGADDYIVKP
                     FDIEELLARIRAILRRQPQKDIIDVNGITIDKNAFKVTVNGAEIELTKTEYDLLYLLA
                     ENKNHVMQREQILNHVWGYNSEVETNVVDVYIRYLRNKLKPYDRDKMIETVRGVGYVI
                     R"
     gene            1063..2418
                     /gene="arlS"
                     /locus_tag="NBH32_002259"
     CDS             1063..2418
                     /gene="arlS"
                     /locus_tag="NBH32_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase ArlS"
                     /protein_id="HCD1515833.1"
                     /translation="MTKRKLRNNWIIVTTMITFVTIFLFCLIIIFFLKDTLHNSELDD
                     AERSSSDINNLFHSKTVKDISALDLNASLGNFQEIIIYDEHNNKLFETSNDNTVRVEP
                     GYEHRYFDRVIKKRYKGIEYLIIKEPITTQDFKGYSLLIHSLENYDNIVKSLYIIALA
                     FGVIATIITATISYVFSTQITKPLVSLSNKMIEIRRDGFQNKLQLNTNYEEIDNLANT
                     FNEMMSQIEESFNQQRQFVEDASHELRTPLQIIQGHLNLIQRWGKKDPAVLEESLNIS
                     IEEMNRIIKLVEELLELTKGDVNDISSEAQTVHINDEIRSRIHSLKQLHPDYQFDTDL
                     TSKNLEIKMKPHQFEQLFLIFIDNAIKYDVKNKKIKVKTRLKNKQKIIEITDHGIGIP
                     EEDQDFIFDRFYRVDKSRSRSQGGNGLGLSIAQKIIQLNGGSIKIKSEINKGTTFKII
                     F"
     gene            2943..5741
                     /locus_tag="NBH32_002260"
     CDS             2943..5741
                     /locus_tag="NBH32_002260"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCD1515834.1"
                     /translation="MTNERKEVSEAPVNFGANLGLMLDLYDDFLQDPSSVPEDLQVLF
                     STIKNDDSIVPALKSTSSQNSDGTIKRVMRLIDNIRQYGHLKADIYPVNPPKRKHVPK
                     LEIEDFDLDQQTLEGISAGIVSDHFADIYDNAYEAILRMEKRYKGPIAFEYTHINNNT
                     ERGWLKRRIETPYKVTLNNNEKRALFKQLAYVEGFEKYLHKNFVGAKRFSIEGVDALV
                     PMLQRTITIAAKEGIKNIQIGMAHRGRLNVLTHVLEKPYEMMISEFMHTDPMKFLPED
                     GSLQLTAGWTGDVKYHLGGIKTTDSYGTMQRIALANNPSHLEIVAPVVEGRTRAAQDD
                     TQRAGAPTTDHHKAMPIIIHGDAAYPGQGINFETMNLGNLKGYSTGGSLHIITNNRIG
                     FTTEPIDARSTTYSTDVAKGYDVPIFHVNADDVEATIEAIDIAMEFRKEFHKDVVIDL
                     VGYRRFGHNEMDEPSITNPVPYQNIRKHDSVEYVFGKKLVNEGVISEDEMHSFIEQVQ
                     KELRQAHDKINKADKMDNPDMEKPAELALPLQADEQSFTFDHLKEINDALLTYPDGFN
                     ILKKLNKVLEKRHEPFNKEDGLVDWAQAEQLAFATILQDGTPIRLTGQDSERGTFSHR
                     HAVLHDEQTGETYTPLHHVPDQKATFDIHNSPLSEAAVVGFEYGYNVENKKSFNIWEA
                     QYGDFANMSQMIFDNFLFSSRSKWGERSGLTLFLPHAYEGQGPEHSSARLERFLQLAA
                     ENNCTVVNLSSSSNYFHLLRAQAASLDSEQMRPLVVMSPKSLLRNKTVAKPIDEFTSG
                     GFEPILTESYQADKVTKVILATGKMFIDLKEALAKNPDESVLLVAIERLYPFPEEEIE
                     ALLAQLPNLEEVSWVQEEPKNQGAWLYVYPYVKVLVADKYDLSYHGRIQRAAPAEGDG
                     EIHKLVQNKIIENALKNN"
     gene            5755..7023
                     /gene="sucB"
                     /locus_tag="NBH32_002261"
     CDS             5755..7023
                     /gene="sucB"
                     /locus_tag="NBH32_002261"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCD1515835.1"
                     /translation="MPEVKVPELAESITEGTIAEWLKNVGDSVEKGEAILELETDKVN
                     VEVVSEEAGVLSEQLASEGDTVEVGQAIAIIGEGSGNASKENSNDNTPQQNEETNNKK
                     EETTNNSVDKAEVNQANDDNQQRINATPSARRYARENGVNLAEVSPKTNDVVRKEDID
                     KKQQAPASTQTTQQAPAKEEKKYNQYPTKPVIREKMSRRKKTAAKKLLEVSNNTAMLT
                     TFNEVDMTNVMELRKRKKEQFMKDHDGTKLGFMSFFTKASVAALKKYPEVNAEIDGDD
                     MITKQYYDIGVAVSTDDGLLVPFVRDCDKKNFAEIEAEIANLAVKAREKKLGLDDMVN
                     GSFTITNGGIFGSMMSTPIINGNQAAILGMHSIITRPIAIDQDTIENRPMMYIALSYD
                     HRIIDGKEAVGFLKTIKELIENPEDLLLES"
BASE COUNT         2701 a         1059 c         1316 g         2111 t
ORIGIN      
        1 atacattaag aaatgttact tgtaagttca aaatcgcaca ttgaaatgat gtgtgaaaat
       61 gtttaaacta cggtcatttt gtgaaaataa agataataac tattcatttt acaatagtga
      121 aaagtcagta tatgacaaca attaatattg cggtaaggcc ttgtgttaca gtattctata
      181 tttaagtact gcaatcagaa ttaacagaat tccattaact gatcattaaa tatttgagtt
      241 aataaataat taatgattgt agcttgaaaa atttaaaaca tggttattga tttgtgataa
      301 aatttaaacg taaacaaact aatttaaaaa gcaactattg tatagaaaaa tacaaaattt
      361 aaaatatatt accttattag aaaaagtcgt aatatgaggt gtacaaatga cgcaaatttt
      421 aatagtagaa gatgaacaaa acttagcaag atttcttgaa ttggaactca cacatgaaaa
      481 ttacaatgtg gacactgagt atgatggaca agacggttta gataaagcgc ttagccatta
      541 ctatgattta atcatattag atttaatgtt gccttcaatt aatggcttag aaatttgtcg
      601 caaaattaga caacaacaat ctacacctat cattataatt acagcgaaaa gtgacacgta
      661 tgacaaagtt gctgggcttg attacggtgc agacgattat atagttaagc catttgatat
      721 tgaagaactt ttagcaagaa ttcgtgcgat tttacgtcgt cagccacaaa aggatattat
      781 cgatgttaac ggtattacaa ttgataagaa tgcttttaaa gtgacggtaa atggcgcaga
      841 aattgaatta acaaaaacag agtatgattt actatatctt ctagctgaaa ataaaaacca
      901 tgttatgcaa cgggaacaaa ttttaaatca tgtatggggt tataatagtg aagtagaaac
      961 aaatgtcgta gatgtttata taagatattt acgaaacaaa ttaaaaccat acgatcgtga
     1021 caaaatgatt gaaacagttc gtggcgttgg gtatgtgata cgatgacaaa acgtaaattg
     1081 cgcaataact ggattattgt taccacgatg attacgtttg tcacgatatt tttgttttgt
     1141 ttaattatta tctttttctt gaaagataca ctgcataata gtgagcttga tgatgcagaa
     1201 cgaagctcaa gcgatattaa taatttattt cattctaaga ctgttaaaga tatatctgca
     1261 ttagacttga atgcatcttt aggtaatttt caagagatta ttatttatga tgaacataat
     1321 aataaattat ttgagacatc gaatgataac acagtgagag ttgaaccagg ttatgaacac
     1381 cgttattttg accgcgtaat aaaaaaacgc tataaaggca ttgaatattt aattattaaa
     1441 gaaccaatca caacgcaaga tttcaaaggg tatagcttgt taattcattc actagaaaat
     1501 tatgataaca tcgtaaaatc attgtatatc attgcgctcg catttggagt gattgcaaca
     1561 attataactg ccacaatcag ttatgtattt tcaactcaaa ttactaaacc gcttgtcagt
     1621 ttatcaaata aaatgattga gattcgacga gatggatttc aaaataaatt gcaattaaat
     1681 acaaattatg aagaaataga taatctagca aatacgttta atgagatgat gagccaaatt
     1741 gaagaatcat ttaatcaaca aagacaattt gttgaagatg catcacatga attacgaaca
     1801 ccattacaaa ttattcaagg tcatttaaat ttgattcagc gatggggaaa aaaagatcca
     1861 gcagtattag aagaatcgtt aaatatttct attgaagaaa tgaatcgtat cataaaatta
     1921 gtcgaagaat tacttgaatt gactaaagga gatgtaaatg acatttcttc tgaagcgcag
     1981 accgtgcata ttaatgatga aattcgctcg cgaatacact cattaaaaca attacatcct
     2041 gattatcaat ttgatacgga tctgacatct aaaaatttag aaattaaaat gaagcctcat
     2101 caatttgaac aattattttt aatctttatt gataatgcaa tcaaatatga tgtgaagaat
     2161 aagaaaatta aagttaagac aaggttaaaa aataagcaaa aaataattga aattacagat
     2221 catggaattg gtattccaga ggaagatcaa gatttcattt ttgatcgctt ttatcgagtg
     2281 gataaatctc gttcaagaag tcaaggcggt aatggactcg gattatctat tgctcaaaaa
     2341 atcattcaat taaacggtgg atcgattaaa attaaaagtg aaattaacaa aggaacaacg
     2401 tttaaaatca tattttaatt atgtttgagc cgtcaatcaa aatcatagga tcaatttttt
     2461 aagtgaggct gggacataaa tcaatgttct atgctttacg aagttatatt ggcagtagtt
     2521 gactgaacga aaatgcgctt gtaacaagct tttttcaatt ctagtcaggg gcccaacaca
     2581 gaagctgacg aaaagtcagc ttacaataat gtgcaagttg gggatgggcc ccaacacaga
     2641 gaatttcgaa aagaaattct acaggcaatg caagttgggg tgggacgacg aaataaattt
     2701 tgcgaaaata tcatttctgt cccactccca agaacgacta taaaacaaat aaacagtggt
     2761 tctttatcat ttctgttgta cgcccaaaat ttacaataaa atacatctat aaacctagaa
     2821 gaatcaacgc ttttgttgat tcttcttttt agcagataaa taggtaaatc tactttaaca
     2881 aataactaaa tagtgatatt attacattgt aagcgtttca acatttttgt ggagggtgta
     2941 aaatgactaa cgaaagaaaa gaagtttcag aggctcctgt aaacttcggt gcgaatttag
     3001 gtctaatgtt agatctatat gatgactttt tacaagatcc atcatctgta ccagaagatt
     3061 tacaagtctt attcagcaca attaagaatg atgactcaat tgtaccagct ttaaaaagta
     3121 caagtagtca aaatagcgac ggcacaatta aacgtgtcat gcgtttaatt gataatattc
     3181 gccaatacgg gcatcttaaa gccgatattt atcctgtaaa tcctccgaaa aggaaacatg
     3241 tacctaaatt agagattgaa gactttgatt tagatcaaca gactttggaa ggtatatcag
     3301 caggaattgt ttcagatcac tttgccgaca tttatgataa tgcttatgaa gcaattttaa
     3361 gaatggaaaa acgttacaaa ggaccaattg catttgagta tacacatatt aataacaata
     3421 ccgaacgtgg ttggttaaaa agaagaattg aaacgccata taaagtaacg ttaaataaca
     3481 acgaaaaaag ggcactattt aaacaattag cgtatgttga agggttcgaa aaatatcttc
     3541 ataaaaactt cgttggtgca aagcgttttt caattgaagg ggtagacgca ctcgtaccga
     3601 tgttacaacg tactattacg attgctgcga aagaaggtat taaaaatata caaataggca
     3661 tggctcaccg tggacgttta aacgttttaa cgcatgtctt agaaaaaccg tacgaaatga
     3721 tgatttcaga atttatgcat acagatccaa tgaaattctt acctgaagat ggtagcttgc
     3781 agttaactgc tggatggact ggtgatgtga aatatcacct tggtggcatt aaaactactg
     3841 attcatacgg tacaatgcag cgtattgcac tggctaacaa tccaagtcac ttggaaattg
     3901 ttgcacctgt tgttgagggg cgtacgagag cagcacaaga tgatacacaa cgtgctgggg
     3961 ctccgacgac tgatcatcat aaagcaatgc caattattat acatggcgat gctgcttatc
     4021 ctggtcaagg aattaacttc gaaacaatga acttaggaaa cttgaaaggc tattctacgg
     4081 gtggttcatt gcatattatt actaacaata gaattggatt tactacagaa ccaattgatg
     4141 cacgttcaac aacttattct acagatgtgg ccaaaggtta tgatgtgcca atattccatg
     4201 tcaatgctga tgacgttgaa gctactattg aagcaattga tattgcaatg gaatttagaa
     4261 aagagtttca taaagacgtc gttattgatt tagtaggtta tcgtcgtttc ggtcataacg
     4321 aaatggatga accatcaatt actaatccag ttccttatca gaatattcgc aaacatgact
     4381 ctgttgaata tgtgtttggt aaaaagcttg ttaatgaagg tgtcatttca gaagatgaaa
     4441 tgcattcatt tatagaacaa gtccaaaagg aactaagaca agcacatgat aaaattaata
     4501 aagctgataa aatggataat ccagatatgg aaaaacctgc agaacttgca ttaccgttac
     4561 aagcagacga acaatcattt acgtttgatc atttgaaaga aataaatgat gcattgttaa
     4621 catatccgga tggctttaac attttgaaaa agttaaacaa agttcttgag aagcgtcatg
     4681 agccgtttaa taaagaggat ggcttagttg attgggcaca agcagaacaa cttgcatttg
     4741 cgacaatttt acaagatggt acaccgattc gcttaactgg tcaagatagt gaacgtggta
     4801 cattcagtca taggcatgcc gtgttacatg atgagcaaac aggtgaaaca tatacacctt
     4861 tacatcatgt tcctgatcaa aaagcgacat ttgatataca caattctccg ctttcagaag
     4921 cagcagtagt tggttttgaa tacggctata atgtggaaaa caaaaaaagc ttcaatattt
     4981 gggaagcgca atatggtgat tttgcaaata tgtcacaaat gatttttgac aacttcttat
     5041 tcagttctcg ctcaaaatgg ggagaacgtt caggattaac attattctta cctcatgcat
     5101 atgagggtca aggtcctgaa cattcatcag caagattaga gcgattttta caattagctg
     5161 ctgaaaataa ttgcacagtt gtcaacttat ctagttcaag taattatttc cacttattgc
     5221 gtgcacaagc agctagttta gattctgaac aaatgcgacc attggttgtt atgtcaccaa
     5281 aaagcttact gagaaataaa acagttgcaa aaccaattga tgaatttact tctggtggat
     5341 ttgagccaat tttgacagaa tcatatcaag cggataaggt tacaaaagtt attttagcaa
     5401 ctggtaaaat gttcattgat ttaaaagaag cattagctaa aaatccagac gaatcagtgt
     5461 tactcgttgc gattgaaaga ttgtatccat tcccagagga agagattgaa gcattactag
     5521 cacaattgcc aaaccttgaa gaagtgtcat gggtacaaga agaacctaaa aatcaaggtg
     5581 catggttata tgtctatcca tatgttaaag tgctagttgc agataaatat gatttaagtt
     5641 atcatggcag aattcaaagg gctgctccag ctgaaggcga tggagaaatt cataaacttg
     5701 ttcaaaataa aattatagaa aatgcattaa aaaataacta gggggaaata agtcatgcca
     5761 gaggttaaag ttccagaatt agcagaatct attacagaag gtaccattgc agaatggttg
     5821 aaaaacgtag gggatagcgt agaaaaaggt gaagctattc ttgaattaga aactgataaa
     5881 gttaatgtcg aagttgtatc tgaagaagca ggtgtattat ctgaacaact tgcaagtgaa
     5941 ggcgacactg tagaagttgg gcaagcaatt gctatcatcg gcgaaggtag tggcaatgct
     6001 tctaaagaaa atagtaacga caatactcca caacaaaatg aagaaacaaa taataaaaaa
     6061 gaagaaacaa caaataattc ggtagataaa gctgaagtca atcaagcaaa tgatgacaat
     6121 cagcaacgta ttaatgctac gccttctgcg cgtcgatatg ctcgtgaaaa tggtgtcaat
     6181 cttgctgaag taagtccaaa aacaaatgat gtggttcgta aagaagatat tgataagaaa
     6241 caacaggcac cggcatcaac acaaacaaca caacaagcac ctgcaaaaga agagaaaaaa
     6301 tacaatcaat atcctacaaa accagtgatt cgtgaaaaaa tgtcacgcag aaagaaaaca
     6361 gctgccaaaa aattattaga ggtatctaat aatacagcta tgttaacaac atttaacgaa
     6421 gttgacatga caaatgttat ggaattgcgt aaacgtaaga aagaacaatt tatgaaagat
     6481 catgatggta ctaaattagg atttatgtca ttctttacta aagcttcagt agcagctttg
     6541 aaaaagtatc cagaagttaa tgcagaaatc gacggcgacg acatgattac gaaacaatat
     6601 tatgatattg gtgtagctgt ttctacagat gatggattat tagtaccatt tgtaagagat
     6661 tgtgataaaa agaattttgc agaaatcgaa gcagaaattg ctaatttagc tgttaaagca
     6721 cgagagaaaa aacttggctt agatgatatg gttaatggtt catttacgat tacaaatggc
     6781 ggtatttttg gatcaatgat gagtacgcca attatcaatg gtaatcaagc tgcaatctta
     6841 ggcatgcatt caattattac aagaccaatt gcgattgatc aagatacaat cgaaaatcgt
     6901 ccaatgatgt atattgcatt aagctatgat catagaatta ttgacggtaa agaagcagtt
     6961 ggattcttaa aaacaattaa agaattaatt gaaaacccag aagacttatt attagaatct
     7021 taatccacaa cacaaaatag tttactattc ctaaaaacgt aatcaaatca ataacaaaca
     7081 gcagtaagat tattttctag tcgaaatatc ttactgctgt actttatttt tataatgctt
     7141 cgtattaaaa tgtgagttta attttgacaa cttaaaaaat atatcat
//
LOCUS       DAHHOJ010000117         7169 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.117, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000117 DAHHOJ010000000
VERSION     DAHHOJ010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7169)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7169
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.117"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..519)
                     /locus_tag="NBH32_002262"
     CDS             complement(<1..519)
                     /locus_tag="NBH32_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4865 family protein"
                     /protein_id="HCD1515836.1"
                     /translation="MHAMQYHVKLPSDYNMEIIRDRVRLNGYKTDGFKNLIIKAYLIS
                     QTTSNCITNTYSPLYLWRSSEGMTEFIFNGFYDNVISSFGWQNINIGVIYSMNITDSV
                     KHSLYALEEYIDIFPTLSLKEIEIKKLFRIFDNAVAEIIIYNPDKWKFVKYSFIENLP
                     KSLNPNIKIYDVL"
     gene            610..1353
                     /locus_tag="NBH32_002263"
     CDS             610..1353
                     /locus_tag="NBH32_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1515837.1"
                     /translation="MIKLNFNDLEIFITVCEEASINKAAIKLRYAQSNISQRISKLEN
                     ELGVVLLFRNQKGAKATKAGEEFLAYSKKVLRDTETIKNKMKYNTMSILCSELLFNYL
                     SESEEIMMSNNSINFISSGNIRKAIEKNNYDKVISFIKINDSNYRLSNVDTMKVTLYS
                     NGSNYDKEPLLINKDEFCPLRKITLDIKLDSQRVMEFDSLAAIINLVEQGKGKALLPM
                     TFENKRDIVQDISKIFEVSYYTYNHIMHH"
     gene            complement(1584..2003)
                     /locus_tag="NBH32_002264"
     CDS             complement(1584..2003)
                     /locus_tag="NBH32_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000039763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1515838.1"
                     /translation="MSKKYILIIIGTIFTIAFIIVGVILIMKYNELMKQKEFYYKQQQ
                     ERITLYLKHNTKEPNTIKSVHFTSLDKGPLDERIIEGYINNNKDDGFVAYMVPAYNHQ
                     FGGDMLRDETVQKLLKPSKDRKSPDQIKKELKEKRSK"
     gene            2547..3149
                     /locus_tag="NBH32_002265"
     CDS             2547..3149
                     /locus_tag="NBH32_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515839.1"
                     /translation="MLISLVIPVFALLVIGGIIWMIIEGIVHISKKNKAIDNFFNQVN
                     KVSETYKFATTFLFLILATAGISQFYLYYIVSAFLFWLVILTFGIAGIIFLMPYGLCF
                     LPFYKQKKKKQTFKKYMVYTTIGLSICLGLSLVLVHTTKIYMDEGGVRYYYGSFVMKQ
                     AGGYAYLALAVLSTLLIVAKKATNKNKEIETVDNTNITER"
     gene            3416..6568
                     /locus_tag="NBH32_002266"
     CDS             3416..6568
                     /locus_tag="NBH32_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA2/NAM7 family helicase"
                     /protein_id="HCD1515840.1"
                     /translation="MSQLLNDTLSAWLLIESLSPGEVNFTAEDILSAEHFKNGAKQAQ
                     LQSFDEYFEIWNAERFIISEEKSETGELIFKFYRHCFRYNEINLKIQDIFDDYSEIHN
                     PNGTHCYGYTFNTDKHGKVIVDSIHIPMIMSALKEIEKNKNANIEEKFNDSVEKFVQK
                     VKEILADEPINEFKLKKMDKAYDEYFSVLNSKKDGLFGHYVAIEYVKDSDLPQPEFNS
                     FFISDIEKARKSPNQTLIDYIEGVEESQRIEVDENKEMFDKFLHPSRLPDGRWPSQTE
                     FRLSLMQQLAVNQITSGNERISSVNGPPGTGKTTLLKDIFAHLVVERGKELAKLNNPK
                     DAFVKTKIHETDDKYVYLLKESIAKYKMVVASSNNGAVENISKDLPKIEEIIRNPEKC
                     KFPKYEQNYANLAHELKDFAEIAEDLIGESAWGLFSGVFGKSTNINQVLSHMLKQDAN
                     DIGFAKLLQNENNRMSYNELMSEWQSHQCAFLEELRHVEMLKEESIRAYDVYKNCESF
                     SKIEQVINSEKTSIEEQIYHLDNETLRDNKEIEDLGDRINYNTKQLETLNELIKSIKE
                     SNKGFINKLKAMFNSEEDESYKDHNKEKQQLLTQQLELEKCKKNKHEDLVSKLKEKEK
                     LIKQLTKVQLQLDELNSQLQELEAYRIESKITIPEKDFWSDNNYDERQVTNLWTSDEL
                     QYRRAMLFLRAMILHKLLLIANNTTIYYAINDFKDRRKLIDANPDKVHNAWNVMHLIF
                     PVVSTTFASFKSMYGGIPKDFIDYLFIDEAGQAIPQAAVGALYRSKKVVAVGDPIQIE
                     PVVTLESHLIDNIRKNYHVPEYLVSKEASVQSVADNANQYGFWKSDATDSNQKTWIGI
                     PLWVHRRCLKPMFTIANQIAYNNKMVLPSNITKVGKTGWYDVKGNAVQKQFVKEHGEK
                     VVGLLADDWIEAIKEGKNEPSSFVISPFSAVQQQIKRMLKQQLPTRIDIERTKINQWV
                     DKSIGTVHTFQGKEAQKVYFVIGTDNTQDGAVNWSCEKPNLLNVAVTRAKKEFYVIGD
                     MQRIQMKPFYETIFKERNVK"
     gene            6643..7125
                     /locus_tag="NBH32_002267"
     CDS             6643..7125
                     /locus_tag="NBH32_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515841.1"
                     /translation="MKKFNNWILNAISGSQTDKNETTEELKGAKFIILYAYSMLVLLA
                     LVISNIFIHILEPKLSITTQIIIVLILIETLIGLRFLKAYDVKRGKDKENKKNSKDFV
                     KLKSILVAILFTSLALTAGTVADIYGFTDLGNTRSDLIVWSIGGIIFGLVCYTMEDKK
                     "
BASE COUNT         2703 a          925 c         1209 g         2332 t
ORIGIN      
        1 tagtacatca taaattttta tatttggatt gagactcttt ggaagatttt caataaatga
       61 atatttcaca aacttccact tatcaggatt ataaattata atttctgcaa ctgcattatc
      121 gaagattcta aacaattttt tgatttctat ttcttttaga gataaagtag ggaaaatgtc
      181 gatatattct tcaagcgcat aaagactatg tttaacagaa tcggttatgt tcattgaata
      241 tatcacacca atatttatat tttgccatcc aaaagaagaa ataacattat cataaaatcc
      301 attgaatata aattcagtca tcccctctga acttctccat aaatataatg gtgaataagt
      361 atttgtaatg caattactag ttgtttgact tattaaataa gcttttataa ttaaattttt
      421 aaatccatca gtcttataac catttaatcg aactctatct ctaattattt ccatattgta
      481 atcactaggc aacttaacat gatattgcat tgcatgcatt tagcataccc ccttttataa
      541 aaaggatagc aataataagt aaaatctcat attatccaat tgtgatatag ttatcataaa
      601 aagtgatagg tgattaaatt gaactttaat gatttggaaa tttttataac tgtatgtgaa
      661 gaagcatcta tcaataaagc tgcaattaaa cttagatatg cacaatctaa tatatctcaa
      721 agaattagca agcttgaaaa tgaattaggt gtagttttgc tttttagaaa tcaaaaaggt
      781 gctaaggcaa ctaaagcagg cgaagaattt ttagcgtata gcaaaaaagt attaagagat
      841 acagagacta taaaaaataa aatgaaatat aacactatgt ctattttatg ctcagaactg
      901 ttatttaatt atttatctga gagcgaagaa attatgatgt cgaataactc aattaatttt
      961 atttctagtg gaaatattag aaaagctata gaaaaaaata attatgataa ggttatttca
     1021 ttcataaaaa ttaatgactc aaattataga cttagtaatg ttgatactat gaaagtaacg
     1081 ctttacagta atggaagtaa ttatgataaa gagcctttac taataaataa agatgagttt
     1141 tgtcctttaa ggaaaataac tttagatatt aagcttgatt cacaacgggt aatggaattt
     1201 gattcattgg cagcaataat aaacttagtt gaacaaggaa aaggaaaggc tttattacct
     1261 atgacttttg agaataaaag agatatagta caggacattt ctaagatatt tgaagttagc
     1321 tactatactt ataatcatat tatgcatcat taattgaaag ttgattgagt ttaaattaca
     1381 agaacgatat taagattcta gtagttagaa tgagcgatgt gatagaaaag aatttttgct
     1441 ttttaattgg taaattcgtt attttaaagt agtggtcttt gtagatagga aaaagcggat
     1501 tcccttatca gccttttcca ctcataaaat ttattattgt aacttttatg aggtgaggag
     1561 ctaaatattg ttcgaactaa attttactta gaccttttct ctttcaattc ttttttaatt
     1621 tgatcaggag atttcctatc ctttgatggt tttagtaatt tctgtaccgt ttcgtctcta
     1681 agcatatcac caccaaactg atgattatat gcaggtacca tgtaagcaac aaaaccgtcg
     1741 tccttattat tattgatata gccttcaatg attctttcat caagaggacc tttgtccaaa
     1801 cttgtaaaat gtaccgactt aatcgtatta ggctcttttg tattatgttt aagatacaat
     1861 gtaatacgtt cttgttgctg cttgtagtaa aattcttttt gtttcataag ctcgttatac
     1921 ttcattataa gaatgacgcc tacaataatg aaggctatag tgaatatagt accgataata
     1981 attaaaatgt atttttttga catgatatca actccgatat tagtaagggc ataggagcaa
     2041 ttgaaatttc caaacaaggt acacgagtgc ttctgaattt atcggcacgc ttttacatag
     2101 atcaaaagaa cgcgcactaa aataatcatt ttatctatgt aacttacatc tttgatacta
     2161 cagaagttcg tgcatttgtt caatcgtctc ataaccatta aatagcgtta attatgaatg
     2221 aagacatcta taacatttat ttatcttata acatagtaca attacaaagc atcacaacgg
     2281 cgactgtact caccttaaat cataacgaca accgctaaaa aatgatttga aaaactaatc
     2341 attaattgcc atactcaact attttgaaaa gtatcaattt cacattatca aattattgag
     2401 tatctcaatt tattgttata cacaaacagt atttttgtta tcatagagtt aataaatata
     2461 ataaattgaa taagtaactt cacaaaaatt agaatgattc aattcaatca caattatttg
     2521 aaaatagagg tgagcaggtg aacgatatgt taattagtct tgtaatccca gtttttgctt
     2581 tgttagttat tggtggtatt atttggatga ttatagaagg tatagtacat atttcaaaaa
     2641 agaataaagc aattgataac ttttttaacc aagttaataa agtaagtgag acatataaat
     2701 tcgctactac ttttttattt ctaatcttgg ctacggctgg tatttctcaa ttttatctat
     2761 attatatagt atcagcgttt cttttttggc ttgtcatact tacctttggt attgcaggta
     2821 tcattttttt aatgccatat ggattatgtt ttctaccgtt ttataagcaa aaaaagaaaa
     2881 aacagacatt taaaaaatac atggtttaca ctacgattgg tttgtcaatt tgtctaggct
     2941 tatctctagt tttggttcac actacgaaaa tttatatgga cgaaggtggc gtaagatact
     3001 attacggtag ttttgtaatg aaacaagcgg gcggttatgc ttatttagct ttagcagtac
     3061 tttcaacgtt gttaattgtt gcgaaaaaag ctacaaataa aaataaagaa atcgaaaccg
     3121 tcgacaatac aaatataacg gaaagataat taagggagtg ctcattcagg agtgctcttt
     3181 tttgatgtcc aaatttagtt gcaaatgaag gcatagaaga aatttttata gtcatttatt
     3241 ggataatgaa ttatggtcat atcgattaat tttattagtg aggattttac aaacatttgt
     3301 tgaataaata tggtaatgat aatatatgaa tatttttgaa aaaacactaa tatgattaaa
     3361 ttatttgtta aaatggcatt aagtaaaaaa caaacaggga acaaggtggg atttcatgag
     3421 tcaattgcta aatgatacgt tatcggcttg gttgttaatt gaatctttaa gtccaggaga
     3481 agtaaatttt acagcggaag atatactctc agctgaacat tttaaaaatg gtgcaaagca
     3541 agcacaactt caaagttttg atgaatattt tgaaatatgg aatgctgaac gctttattat
     3601 atcagaagaa aaatcagaga ctggggaact tatatttaaa ttttatagac attgcttccg
     3661 ctataatgaa attaatttga aaattcagga tatttttgat gattattctg agattcataa
     3721 tccaaatggg acacactgtt atggttacac atttaacaca gataaacacg gcaaagtgat
     3781 agttgattct atacatattc cgatgattat gagtgcatta aaagaaattg aaaagaacaa
     3841 aaatgccaat atagaagaaa aatttaatga ttctgttgaa aaatttgttc aaaaagtaaa
     3901 agaaatttta gcagatgaac caattaatga atttaaattg aagaagatgg acaaagctta
     3961 tgatgagtac ttttctgtat taaattcaaa gaaagatgga ttatttggac attatgtagc
     4021 aatagaatat gtgaaagata gtgatttacc acagccggaa tttaacagct tcttcataag
     4081 tgatattgag aaagcaagaa aatctcccaa ccaaacttta attgattaca ttgaaggtgt
     4141 agaagaaagt cagcgcatag aagtagatga aaataaagaa atgtttgaca aatttttaca
     4201 tccttcacgt ttgcctgatg gacgatggcc atcacagact gagtttagat tgtctttaat
     4261 gcaacaactt gctgtaaacc aaattacgag tggtaatgaa agaataagtt cagttaatgg
     4321 gccaccaggt acaggtaaga ctactttatt aaaagatata tttgctcatc tagtagttga
     4381 aagaggtaaa gagttagcta aactaaataa tcctaaagat gcatttgtca aaacaaaaat
     4441 tcatgaaacg gatgataaat acgtatactt actaaaggaa tctattgcca aatataagat
     4501 ggtagtcgca tctagtaata atggagctgt tgaaaatata tctaaagatt taccgaaaat
     4561 tgaagaaatt ataagaaatc ccgaaaaatg taaattccct aaatatgaac agaattatgc
     4621 aaatttagca catgaattaa aagattttgc tgaaatagct gaagatttga ttggtgaaag
     4681 tgcctggggc ttattttctg gagtttttgg taaaagtact aatattaacc aagtattgag
     4741 tcatatgtta aaacaagatg cgaatgatat tggctttgct aaattactac aaaatgagaa
     4801 taatcgtatg agttataacg agttaatgag tgaatggcaa tcacatcaat gtgcattttt
     4861 agaagagttg aggcatgttg aaatgttaaa agaagaatct attagagcat atgatgttta
     4921 taaaaattgt gagtctttct ctaagattga acaggttatt aatagtgaaa aaacaagtat
     4981 tgaagaacag atatatcatt tagataatga aacgttacga gacaataaag aaattgaaga
     5041 tttgggtgat cgaattaatt ataatacaaa gcaactcgaa actttaaatg agttaattaa
     5101 atccatcaaa gaaagcaaca aaggttttat taacaaactg aaagcgatgt ttaattcaga
     5161 agaagatgaa agctataaag atcataataa agagaagcaa caattattaa cacaacagtt
     5221 agagttagag aaatgtaaaa aaaacaaaca tgaagacctt gttagcaaac taaaagaaaa
     5281 agagaaatta attaaacaat taactaaagt acagttgcaa ttagacgagt taaattcaca
     5341 attacaagag ttagaagcat atcgtattga gtcaaaaatt acaattccag aaaaagattt
     5401 ttggagtgac aacaattatg atgagcgcca agttactaat ctgtggacga gtgacgaact
     5461 tcaatacaga cgtgccatgc tttttttaag agcaatgata ttgcataaat tattattgat
     5521 tgctaataat acaactattt attatgcgat taatgatttt aaagatagaa ggaaattaat
     5581 tgatgcaaat ccagataaag tacacaacgc atggaatgtg atgcatttaa tatttccagt
     5641 agttagtacg acgtttgcaa gctttaaatc tatgtatggg ggcataccaa aagatttcat
     5701 agactactta tttattgatg aagcaggaca agcaatacct caagcagctg tgggagcatt
     5761 atatcgttca aaaaaagttg tagctgtagg tgatccgatt caaatagaac cggttgtgac
     5821 tttagaaagt catttaattg ataacattcg taaaaattat catgttccgg aatatctagt
     5881 ttctaaagaa gcttctgtgc agtctgttgc agacaacgcc aatcaatatg gcttttggaa
     5941 atctgatgct actgatagta atcaaaaaac atggataggc atacctttat gggtgcacag
     6001 acgatgttta aaacctatgt tcacgatagc taaccaaatc gcttataata ataaaatggt
     6061 gttgccaagt aatattacaa aagtaggtaa aacaggttgg tatgacgtta aaggaaacgc
     6121 agttcaaaaa caatttgtga aagagcatgg tgaaaaagta gtgggattat tagctgatga
     6181 ttggattgaa gcaattaagg aaggtaaaaa tgaaccgagc tcatttgtaa tatcgccttt
     6241 ttcagcagta cagcaacaga ttaaacgtat gttaaagcaa caactaccga ctagaattga
     6301 tattgaacgt acaaaaatta atcaatgggt cgataaatcc attggtactg ttcatacttt
     6361 tcaaggtaaa gaggctcaga aggtgtattt tgtaataggt actgataata cccaagatgg
     6421 tgctgtgaac tggtcatgcg aaaaaccaaa cttgttaaac gttgcagtga caagagctaa
     6481 gaaagagttt tatgtaattg gcgacatgca aagaatacag atgaaaccat tttatgagac
     6541 gatttttaaa gaaagaaatg taaaatgaaa ttgaaataac agtattactt gatacttaat
     6601 tatcgagcaa tttatacatt ttaaaaggag caaaattgaa taatgaagaa atttaacaac
     6661 tggatattaa atgcaataag tggatctcaa acagacaaga atgaaacaac tgaagaatta
     6721 aaaggggcaa aatttatcat tttatatgca tattcaatgc tcgttttgct tgcgttagta
     6781 atttctaaca tattcattca cattttggag cctaaactat caattaccac tcaaatcatc
     6841 atcgttttga ttttaattga aacactaatt ggactgcgtt tcttgaaagc gtacgatgtt
     6901 aagcgtggca aagataaaga aaataagaaa aatagtaagg atttcgttaa actaaaatca
     6961 attttagtag caattttatt tacatcattg gcgctgacag caggcactgt agctgatata
     7021 tacggtttca ctgacttagg aaatactaga agtgatttaa tcgtttggag cataggtggt
     7081 attatatttg gcctcgtatg ttacacaatg gaagataaaa aataacgata aggagctggc
     7141 gattataaag ctagctcctt ttttaactt
//
LOCUS       DAHHOJ010000118         7041 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.118, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000118 DAHHOJ010000000
VERSION     DAHHOJ010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 7041)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7041)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7041
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.118"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            77..910
                     /locus_tag="NBH32_002268"
     CDS             77..910
                     /locus_tag="NBH32_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HCD1515842.1"
                     /translation="MNKTVKDLILVVLGSFIFAAGVNAFIISGNLGEGGVTGLAIILY
                     YAFHISPAITNFLVNAVLIAIGYKFLSKRSMYLTILVTILISIFLSLTESWQVETGNS
                     IVNAIFGGVSVGLGIGVIILAGGTTAGTTILARIATKYLDVSTPYALLFFDMIVVAIS
                     LTVIPLDKVLVTVISLYIGTKVMEYVIEGLNTKKAMTIISTNPDKLAKAIDEQIGRGL
                     TILNGHGYYTREEKDVLYVVISKTQVSKAKRLIKQIDKDAFLVIHDVRDVYGNGFLAD
                     E"
     gene            1191..1988
                     /locus_tag="NBH32_002269"
     CDS             1191..1988
                     /locus_tag="NBH32_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515843.1"
                     /translation="MNRLHGQQVKIGYGDNTIINKLDVEIPDGKVTSIIGPNGCGKST
                     LLKALSRLLAVKEGEVFLDDENIHTQSTKEIAKKIAILPQSPEVADGLTVGELVSYGR
                     FPHQKGFGRLTAEDKKEIDWAMEVTGTDTFRHRSINDLSGGQRQRVWIAMALAQRTDI
                     IFLDEPTTYLDICHQLEILELVQKLNQEQGCTIVMVLHDINQAIRFSDHLIAMKEGDI
                     IATGSTEDVLTQEILEKVFNIDVVLSKDPKTGKPLLVTYDLCRRAYS"
     gene            2024..3028
                     /locus_tag="NBH32_002270"
     CDS             2024..3028
                     /locus_tag="NBH32_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1515844.1"
                     /translation="MTNRENPTPLKFLYYIIGLSMILLIILFISTLIGDAKIQASTII
                     EAIFNYNPSNQQQNIINEIRIPRNIAAVIVGMALAVSGAIIQGVTRNGLADPALIGLN
                     SGASFALALTYAVLPNTSFLILMFAGFLGAILGGAIVLMIGRSRRDGFNPMRIILAGA
                     AVSAMLTALSQGIALAFRLNQTVTFWTAGGVSGTTWSHLKWAIPLIGIALFIILTISK
                     QLTILNLGESLAKGLGQNVTMIRGICLIIAMILAGIAVAIAGQVAFVGLMVPHIARFL
                     IGTDYAKILPLTALLGGILVLVADVIARYLGEAPVGAIISFIGVPYFLYLVKKGGRSI
                     "
     gene            3025..4041
                     /locus_tag="NBH32_002271"
     CDS             3025..4041
                     /locus_tag="NBH32_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCD1515845.1"
                     /translation="MISSNNKRRRWIALAVFSILLFLGCTWSITSGEYNIPVERFFKT
                     LIGQGDAIDELILLDFRLPRMMITILAGAALSISGAIVQSVTKNPIAEPGILGINAGG
                     GFAIALFIAIGKINADNFVYVLPLISILGGITAALIIFIFSFNKNEGVTPASMVLIGV
                     GLQTALYGGSITIMSKFDDKQSDFIAAWFAGNIWGDEWPFVIAFLPWVLIIIPYLLFK
                     SNTLNIIHTGDNIARGLGVRLSRERLILFFIAVMLSSAAVAVAGSISFIGLMGPHIAK
                     RIVGPRHQLFLPIAILVGACLLVIADTIGKIVLQPGGVPAGIVVAIIGAPYFLYLMYK
                     TKNV"
     gene            4275..5243
                     /gene="dhaK"
                     /locus_tag="NBH32_002272"
     CDS             4275..5243
                     /gene="dhaK"
                     /locus_tag="NBH32_002272"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCD1515846.1"
                     /translation="MMKKLINKKETFLTDMLEGLLIAHPELDLIANTVIVKKDKKEHG
                     VAIVSGGGSGHEPAHAGFVAEGMLDAAVCGEVFTSPTPDKILEAIKAVDTGDGVLLVV
                     KNYAGDVMNFEMAQELAEMEGINVETVIVRDDIAVTNEAQRRGVAGTVFVHKLAGYLS
                     EKGLALGEIKSRVEALLPEIKSIGMAIEPPLVPTTGKYGFDIEDDKMEIGIGIHGEKG
                     IHREEVKDIDHIVRTLLDELYKEVTANEVIVMVNGMGGTPLSELNIVTKYIQQNLTAR
                     TVNVVKWFVGDYMTSLDMQGFSITIVPNKPEYLEAFLAPTTSQYFK"
     gene            5285..5869
                     /gene="dhaL"
                     /locus_tag="NBH32_002273"
     CDS             5285..5869
                     /gene="dhaL"
                     /locus_tag="NBH32_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HCD1515847.1"
                     /translation="MKVNDMKARLLNLEETFKKHESELTELDRAIGDGDHGVNMVRGF
                     SSLKEKLDDSSMQSLFKSTGMALMSNVGGASGPLYGFSFVKMSAVTKDDMDNQDFTTL
                     IQAFAEAVESRGKVTLNEKTMYDVVARAAEKLKNGETLTFNDLQQLADNTKDMVATKG
                     RAAYFGEESKGYIDPGAQSMVYILNALIGDEDNA"
     gene            5862..6224
                     /locus_tag="NBH32_002274"
     CDS             5862..6224
                     /locus_tag="NBH32_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HCD1515848.1"
                     /translation="MPKIILVSHSKEIANGTKSLLKQMAGDVDIIPIGGLPDGSIGTS
                     FDIIQEALTELDDDAMCFYDIGSSEMNVDMAIEMYDGNHRVLKVDAPIVEGSFIAAVK
                     LSIGGSIDDALAEIKQSF"
     gene            6340..6837
                     /locus_tag="NBH32_002275"
     CDS             6340..6837
                     /locus_tag="NBH32_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515849.1"
                     /translation="MQHLIKKHVLNGEFDLVRQLMSETDFMEFEEAYISSAHEVESMM
                     FYTCILDMIKYEESSEMHDLAFLLLVYPLSEYEGALDSAYYHADASIKLTDGKEVKSL
                     LQMLLLHAIPTPVISDKKAFDIAKQILKLDPNNNVARNVLKDTAKRMDNVVVDINELH
                     QRNAR"
BASE COUNT         2362 a          978 c         1408 g         2293 t
ORIGIN      
        1 ttccatagag ttgtgacagc atttttgttg tgctactatt tttatagtct aataaatata
       61 taaaggggat ggtttcgtga ataaaacggt taaagattta atactagttg tcttaggttc
      121 atttatcttt gctgcaggtg taaatgcatt tattatttct ggtaacttag gtgaaggcgg
      181 ggttacaggt ttagcaatta ttttatatta tgcgtttcat atttcaccag ccatcactaa
      241 cttcttggtc aacgcagtat tgattgccat aggttataaa tttttgagta agagaagtat
      301 gtacttaact attcttgtaa caattcttat ttcaatattt ttgagtttaa cagaatcatg
      361 gcaagttgaa actggaaaca gcattgtgaa tgccattttt ggtggtgtaa gcgttggact
      421 aggaatcgga gtaattatcc ttgcaggcgg tacaacagca ggtacaacaa ttttggcgag
      481 aattgcaacg aaatacctcg atgtaagcac gccatatgct ttgcttttct tcgatatgat
      541 cgttgttgca atttcactta cagttattcc acttgataaa gtattagtaa cagtaatatc
      601 actttatata ggaacaaaag tgatggaata tgtcatagaa ggtttaaaca ctaaaaaagc
      661 tatgacgatt atttcaacta atcccgacaa acttgccaaa gcaatagacg agcaaattgg
      721 aagaggttta accattttaa acggacatgg ctattatacg cgtgaagaaa aagatgtctt
      781 atacgttgtt atttctaaaa cacaagtttc aaaagcaaag cgattaatta aacaaatcga
      841 taaagatgca ttccttgtaa ttcatgatgt aagagatgtc tatggtaatg gctttcttgc
      901 agatgaataa ataaatggta tgagcacaca tacttaaata gaagtccacg gacaagtttt
      961 tgaactatga agacttatct gtgggcgttt tttattttat aaaagtaata tacaagacat
     1021 gacaaatcga gctatccaat ttaaaaagta atgttagtca ataagattga aaaatgttat
     1081 aatgatgttc atgataatca ttatcaattg gggtgccttt gaaaattgat aatttaaaaa
     1141 tagaaattat tttttataaa cagaaagaat ttaatgaaag tagggaaatt atgaatcgtt
     1201 tgcatggaca acaagttaaa attggttacg gggataacac gattataaat aaattagatg
     1261 ttgaaatacc agatggcaaa gtgacgtcaa tcattggtcc taacggctgc gggaaatcta
     1321 ctttgctaaa ggcattgtca cgtttattgg cagttaaaga aggtgaagta tttttagatg
     1381 atgaaaatat tcatacacaa tctacgaaag agattgcaaa aaaaatagct attttacctc
     1441 aatcacctga agtagcagat ggcttaactg ttggagaatt agtttcatat ggtcgttttc
     1501 cacatcaaaa aggatttggt agattaactg ctgaggataa gaaagaaatt gattgggcaa
     1561 tggaagttac aggaactgat acatttcgac accgttcaat caatgattta agtggtggtc
     1621 aaagacaacg tgtttggatt gcaatggcat tagcacaaag aactgatatt atctttttag
     1681 acgaaccaac aacatattta gatatctgtc atcaattaga aatactagaa ttagttcaga
     1741 agttaaatca ggaacaaggt tgtacaattg tcatggttct tcatgatatt aaccaagcga
     1801 ttcgtttctc agatcatctt attgcgatga aagaaggcga tatcatcgct acaggttcaa
     1861 cagaagacgt attaacacag gaaattttag aaaaagtttt taatattgat gttgttttaa
     1921 gtaaagatcc taaaactgga aaacctttac tggtaactta tgacttatgt cgcagagctt
     1981 attcttaatt aagtaagtta atatgataaa aaggacaatt aacatgacaa atagagagaa
     2041 tccaacgcca ttgaagtttt tatactatat tataggttta agtatgatac tactaatcat
     2101 actatttatt tctacattaa taggtgacgc caaaattcaa gcctctacaa ttatagaggc
     2161 tatttttaat tataatccta gcaatcagca gcaaaacatc atcaatgaga ttaggattcc
     2221 cagaaatata gcagcagtaa ttgtgggtat ggcgcttgca gtttctggtg cgattataca
     2281 aggtgttact cgtaatggtc ttgctgatcc ggcgctcata ggtttaaatt caggtgcttc
     2341 atttgcttta gcattaacat atgcagtttt accaaacact tcatttttaa tattgatgtt
     2401 tgctggattt ttaggtgcta ttctaggagg ggctattgta ttaatgatag gccgatctag
     2461 acgtgatgga tttaatccga tgcgtattat tttagcgggt gcagcagtaa gtgctatgtt
     2521 aacagcgcta agtcaaggta ttgcattagc ttttagacta aatcaaacag taacattttg
     2581 gactgctgga ggcgtttcag gcacaacatg gtcacacctt aagtgggcaa ttccattaat
     2641 tggaattgcg ttattcatta tattaacaat tagtaaacaa cttaccattt taaatcttgg
     2701 tgaatcatta gctaaaggtt taggtcaaaa tgtaacaatg atcagaggca tatgtttaat
     2761 tattgctatg attctagcag gtatagcagt tgctatcgct ggacaagttg catttgtagg
     2821 tttgatggta cctcatatag caagattttt aattggaact gattatgcta aaattctacc
     2881 attaacagcc ttgttgggtg ggatactcgt gcttgttgcc gatgtgatag cacgatattt
     2941 aggagaagcg cctgttggtg caatcatttc atttatcggt gttccttact ttttatattt
     3001 agttaaaaaa ggaggacgct caatatgatt agttcaaata ataaacgcag acgatggatt
     3061 gcactggctg tttttagcat tctactattt ctaggttgta cttggagtat cacctcaggt
     3121 gaatacaaca taccagttga aagatttttc aaaactttaa ttggacaagg tgatgccatt
     3181 gatgagctaa tcttattaga tttcaggtta cctcggatga tgattactat tttggcaggc
     3241 gcagcgctta gtattagtgg tgcaatagtg caaagtgtca caaaaaatcc aatagctgaa
     3301 cccggtatat taggtattaa tgcaggcggc ggatttgcaa tcgcattatt tattgcaatt
     3361 ggtaaaatta atgctgacaa ctttgtttat gtactgccgt taataagtat actaggtggt
     3421 atcaccgctg cattgattat ttttattttc agttttaata aaaatgaagg tgttacacct
     3481 gcgagtatgg tattaatagg tgtaggttta caaacagcat tgtatggtgg ctcaattaca
     3541 attatgtcaa aatttgatga taagcaatct gatttcatcg ctgcttggtt tgcaggtaat
     3601 atttggggtg acgaatggcc atttgtcatt gcatttttac cgtgggtgtt gattattatt
     3661 ccttacttac tatttaaatc gaatacacta aatattattc atacgggtga taatattgca
     3721 cgaggtctag gtgtaaggtt aagcagagaa cgtttaatat tattctttat cgcagtgatg
     3781 ttatcatctg ctgctgtagc agtagcaggt tcaatttcgt ttatcggatt aatgggcccg
     3841 catattgcca aacgtatcgt tggaccacgt caccagttgt ttttaccaat tgccatttta
     3901 gtaggggcat gtttacttgt tatagctgat acaattggca aaattgtatt acaaccaggt
     3961 ggggttccag caggtattgt cgtagcaatt attggtgcac cgtatttctt atatttaatg
     4021 tacaaaacga aaaacgtata gtgtcaaagg acacaacata ttgctatgaa aggcacttta
     4081 ttataaggct tttcatagca ttttttattt aatgagtcac tcaagactat ttactttttc
     4141 aataatgaac cattaagtta tcaagaggat cttatcaaaa atatatttga taacggtatc
     4201 aggttaaccc tttatgatag cgcattcatt tattctgttt tatactatga ctgataatac
     4261 caaggaggta caacatgatg aaaaagttaa tcaataaaaa agaaacattt ttaactgata
     4321 tgcttgaagg attgttaatt gcgcacccag agttagatct gattgctaat acagttattg
     4381 taaaaaaaga taagaaagaa catggtgtag caatagtctc tggaggtgga agcggacatg
     4441 aacctgcgca tgccggtttt gttgcagaag gtatgctaga tgcagcggtt tgtggcgagg
     4501 tatttacttc acctacacca gataaaatat tagaagctat taaagcagta gatacaggtg
     4561 atggtgtatt actagttgtg aaaaactatg caggtgacgt gatgaatttt gaaatggcac
     4621 aagagcttgc agaaatggaa ggtataaatg ttgaaacagt tattgttcgt gacgacattg
     4681 ctgtgacaaa tgaagcacaa cgtcgtggtg ttgcaggaac agtgtttgtt cataagcttg
     4741 ccggttatct ttcagaaaaa gggctcgcat taggcgagat taaatcgcgt gtagaagcgt
     4801 tgttacctga aattaaaagt attggtatgg caattgagcc accgcttgtt ccaactactg
     4861 gaaaatatgg ctttgatatt gaagacgaca aaatggaaat tggcattggt atacatggtg
     4921 aaaaaggtat tcatagggaa gaagtaaagg atattgatca tattgttaga acattattag
     4981 acgaattgta taaagaagtt actgccaatg aggtcatagt aatggtaaat ggaatgggag
     5041 gtacgccgtt atctgaatta aatatcgtaa ctaaatatat tcaacaaaat ttaactgcaa
     5101 gaacggttaa tgttgttaaa tggtttgttg gtgattatat gacatcttta gacatgcaag
     5161 gtttttctat aactatcgtg cctaataaac cagaatatct ggaagcattt ttagcaccaa
     5221 caacaagtca atactttaaa taagcagtga atatgaataa atatacattt atgaggtggc
     5281 acaaatgaaa gtgaatgata tgaaagcgcg tttattaaat ttagaagaaa cgtttaaaaa
     5341 acatgaatct gaattaactg aattagatcg agcaattggt gatggtgacc acggggttaa
     5401 catggttcga gggtttagta gtcttaaaga aaaacttgat gatagctcaa tgcaatcatt
     5461 gttcaaatcg actggtatgg cattgatgtc aaatgttggc ggtgcatcag gaccactgta
     5521 tggctttagc tttgttaaaa tgtctgcagt tactaaagat gatatggata atcaagattt
     5581 cactacacta attcaagcat ttgccgaagc ggtcgaatca cgtgggaaag ttactttaaa
     5641 tgaaaagaca atgtatgatg tagtagcacg agcagcagag aagcttaaaa atggtgaaac
     5701 tttaacattt aatgatttac aacaattagc agataataca aaagatatgg tagcaacgaa
     5761 aggtagagct gcatattttg gagaagaatc aaaaggttat atagatccag gtgctcaaag
     5821 tatggtttat attttaaacg ctttgatagg agatgaagat aatgcctaaa attatacttg
     5881 ttagccatag taaagaaatt gcaaatggta caaaatcttt gttaaagcaa atggctggtg
     5941 acgttgatat tataccaatc gggggattac cagatggttc aattggaact tcatttgata
     6001 tcattcaaga agctttaact gaattagatg atgatgcaat gtgtttttac gatattggat
     6061 cttcagaaat gaatgtagat atggcaattg aaatgtatga tggtaatcat cgtgtgttaa
     6121 aagttgatgc accaattgtt gaaggcagtt ttatcgcagc agtaaagctg tcaatcggcg
     6181 gttcaattga tgatgcatta gcagaaatca aacaatcatt ttagttaaaa tttactaata
     6241 atgaaaaatg taaacctttt tcaaatgaaa ctttataaaa aatatgatag tatatatgta
     6301 aatgtttaat aaaatctgga gaaataggag gacattgcca tgcaacacct tataaaaaaa
     6361 catgtattga atggcgagtt tgatttagta cgacaattga tgtccgaaac agattttatg
     6421 gaatttgaag aagcatatat ttcaagtgcg catgaagtag aaagtatgat gttttataca
     6481 tgtattttag atatgattaa gtacgaagaa tcatctgaaa tgcatgactt agcattttta
     6541 ttgcttgtgt atccactaag tgaatatgaa ggtgctttgg attctgctta ttatcatgca
     6601 gacgcttcca taaaacttac tgacggcaaa gaagttaaaa gtttgttaca aatgttatta
     6661 ttgcatgcga taccaacacc tgttatttca gataagaagg cttttgatat cgccaagcaa
     6721 attttaaaat tagatcctaa taataatgtt gctcgtaacg tcttaaaaga cactgccaaa
     6781 cgtatggaca acgttgttgt tgatataaat gaattacacc aacgtaatgc acgttaatta
     6841 catttcaatt atattagctt aataatagtt ttaacatttg gttgggttgg gacatatgtt
     6901 ccagcctttt tttatactta aaaactaacg aagtgtactt gtgtgcacaa atggttttta
     6961 tacaacattt tataaattta tacattttaa taaagaacat acgatagatg gtttaaacct
     7021 tattaactga gagattttga t
//
LOCUS       DAHHOJ010000119         6858 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.119, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000119 DAHHOJ010000000
VERSION     DAHHOJ010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6858
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.119"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(109..801)
                     /locus_tag="NBH32_002276"
     CDS             complement(109..801)
                     /locus_tag="NBH32_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1515850.1"
                     /translation="MNYSPKQGVRSHGLPHDPFKSSTVPRPIGWISTVSKDGKDNLAP
                     YSQYQNLTWDPPMVMFAANQSVLGDHERKDTVKNAEETGWFVWNMATYDLREAVNLSS
                     KALPPEEDEFEYAGVTKEACIEAPGYRVKESPVHFECEYVQTIRIPTGDLVSTVDIVI
                     GRVAQVHIDDKVILDNGKLDIKSIKPIARLGYYDYTVVNEIFEMKAPSASKEELAGLE
                     GRNFDNQSDDKE"
     gene            1133..1768
                     /locus_tag="NBH32_002277"
                     /pseudo
     CDS             1133..1768
                     /locus_tag="NBH32_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226788.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            1755..2057
                     /locus_tag="NBH32_002278"
     CDS             1755..2057
                     /locus_tag="NBH32_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515851.1"
                     /translation="MRTNNHITDLDAIELSSAWNYNSKLVTGKNALLSDSLLRDIKVL
                     SSNAKGDADMLETELHAVINAMSQNKGKSVDALKNSTASLKNNLTKLKEQLDKTTH"
     gene            2208..2567
                     /locus_tag="NBH32_002279"
     CDS             2208..2567
                     /locus_tag="NBH32_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515852.1"
                     /translation="MILRYVTNLKLAKELYQASKPKLQEDKGMIELFEHTFGLQKELV
                     KYVGIAEATTAALYSASFINKNISRIASLSTIGILSVATYKHFEAGQGKKGAQHALDL
                     LGLATLSLLDTFSCNKK"
     gene            complement(2652..3263)
                     /locus_tag="NBH32_002280"
     CDS             complement(2652..3263)
                     /locus_tag="NBH32_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HCD1515853.1"
                     /translation="MYTFDAQSLTARENYKLLIGSIIPRPIAFVTTLNQDASVNAAPF
                     SFFNIVNNHPPMIAIAVQRAGGKRKDTALNIERTGDFVVHITDEDNVQDINETAAPLA
                     YGDSELSRTKLSLLTSTTIKTPGIKDAKMRFECKLSQMILLGDVLDGADLIIGEIVTY
                     HIDDSIYEGDFKINPHALQAVSRLAGNDYAKLGGLFTIDRPEK"
     gene            complement(3342..3818)
                     /locus_tag="NBH32_002281"
     CDS             complement(3342..3818)
                     /locus_tag="NBH32_002281"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase"
                     /protein_id="HCD1515854.1"
                     /translation="MAVVYVAGGCLWGVETFFATIPGIIHTEAGRANGRSSKLDGPYD
                     GYAECVKLHFDDRMLTITDIMNYLFEIIDPYSVNQQGNDIGEKYRTGLYSCVDDHLIE
                     ARQFIERRKDRDQIAVEVLPLSNYIKSAEEHQQHLEKYPEDMHMCHISKDLLNKYK"
     gene            complement(3821..4321)
                     /locus_tag="NBH32_002282"
     CDS             complement(3821..4321)
                     /locus_tag="NBH32_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515855.1"
                     /translation="MVLRKVTMQDLDQIIALENIGFSPEEAATPEALKLRIEQIQETF
                     IVAEKNDEVIGYINGPVIKERYISDDLFKNVSTNNSEGGYISVLGLVVAPNYQGQGIA
                     GRLLNYFENLAKNQHRHGVTLTCRESLISFYEKYGYRNEGVSESCHGGIKWYNLVKNI
                     KKDDEV"
     gene            complement(4556..5323)
                     /locus_tag="NBH32_002283"
     CDS             complement(4556..5323)
                     /locus_tag="NBH32_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HCD1515856.1"
                     /translation="MIDIHNHILVDIDDGPKTIEKSIALLKQAKDEGVTSIVATPHHL
                     HPRYDNTFQQVLVKLAELRTHPEVQALDIKLFPGQEIRITDSILQGLDNGSIQGINRS
                     KYLLIEFPTGEVPHYTKQLFFEIQSRGYIPIIAHPERNRSIAKNPEILYELVANGALS
                     QLTSSSLVGGFGKNIQKLSLQFIECNLAHFVASDAHSCDQRPFLMQELFHNHKLKKYS
                     NDIEALLRNASSVINDNFVYLDRPTKPGKVKSFLKWF"
     gene            complement(5320..6012)
                     /locus_tag="NBH32_002284"
     CDS             complement(5320..6012)
                     /locus_tag="NBH32_002284"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HCD1515857.1"
                     /translation="MTNTRRSTSSLIVHEQPKSPISEKFRGIRSNIMFANPDSAVQSI
                     VITSEAPGAGKSTIAANLAVAYAQAGYKTLIVDGDMRKPTQHYIFNLPNNEGLSSLLL
                     NWSTYQDSIISTEIEDLDVLTSGPIPPNPSELITSRAFANLYDTLLMNYNFVIIDTPP
                     VNTVTDAQLFSKFTGNVVYVVNSENNNKDEVKKGKELIEATGAKLLGVVLNRMPKDKS
                     ASYYAYYGTDES"
     gene            complement(6029..6691)
                     /locus_tag="NBH32_002285"
     CDS             complement(6029..6691)
                     /locus_tag="NBH32_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide biosynthesis protein
                     CapA"
                     /protein_id="HCD1515858.1"
                     /translation="MKEKFDLVKLLNILKKNIKLLLILPAICLVVSAALTFFVMPDKY
                     TASTQILVNMKKSSSDLAFQNVQSSLQSVNTYTEIIKSPRILDKVSREFDGQYSTAEL
                     NSFLKVTNQTNSQIITVSVTTGNKSESDKIVNKISKVFAHDMPKIMSVDNVTILSSAH
                     DNAVKVSPIVSVNLVISIIVGIVLAILIIFLKELLDKRIKTEEDVESQLGLPILGSIQ
                     KF"
BASE COUNT         2258 a         1235 c          977 g         2388 t
ORIGIN      
        1 ttaaaattat aaatatagcg acatatcgct tcccggtttt gaaatataaa aatacatcga
       61 aactaatttc aacgtgtagt ttcgatgtat tgaaaatgat tgaattcatt attccttgtc
      121 atctgattga ttatcaaaat ttcttccttc taaacctgca agctcttctt tagaagctga
      181 tggtgctttc atttcaaata tttcattcac aaccgtgtaa tcataatatc ctaacctcgc
      241 aataggttta atcgacttaa tgtctaattt accattatca agaatgacct tatcgtcaat
      301 atgaacctga gcaactcttc ctataacaat atcaactgtt gatacaaggt ctcctgtcgg
      361 aatacgaatc gtttgaacgt actcacattc aaaatgtact ggcgattctt tgactcgata
      421 acctggtgct tctatacatg cttctttcgt cacacctgca tattcaaatt catcctcttc
      481 aggtggtagt gccttcgacg ataaattaac tgcttctctt aaatcatacg ttgccatatt
      541 ccacacaaac caccctgtct cttcagcatt tttcactgta tctttacgtt cgtgatcacc
      601 aagaacggat tgatttgctg cgaacataac cataggcgga tcccaagtta agttttgata
      661 ctgactataa ggtgctaaat tatctttccc atctttcgat acagtagaga tccaccctat
      721 tggacgtggt actgtactac ttttaaatgg atcgtgtggt aagccatgac tccttacacc
      781 ttgcttcggc gaataattca ttctatcctc accccttatt agtaattaca tttaaggtta
      841 cgccctcttt acataagcgt ctaatataaa taatcaattt atttataagt agaaactata
      901 tatgacgtgg tttcttataa tttgcgttct tgattcgaaa atttcagata aagatttatg
      961 caattaatat ttatgatatc tttttgtaaa tttaattaat tatagttact tcaatcatga
     1021 ttagtttata ataatattga ataacaatat tttagttttt gtataatttg cctatcaact
     1081 gaaaaaacag ctaatacaaa ggagctaatg aagatatgag aaaaattatg cgaaccttat
     1141 tatgcacatc aatcatattc aactccaatt actttttgtt gagtctggaa atggcataac
     1201 tatttcaaca tttacacctg gtcatataaa tgaaacatca gcggcagttg cagccatgat
     1261 gtacaaacgt tatgaacatg aaattgaaca agctcgatta aaattcaaat cagaagttga
     1321 gaaaaatggt tattatgcca tgaataaaaa acttcaaaag aagcaagaat tcaatggtgt
     1381 tactaaacaa tttttgaatt tcaacacaga cagtatcgat gacttaaaaa agtttaaaca
     1441 agaattcaaa cctgttatgc acttaaaagg agaagcgttt aatcaacaat tacaatcttt
     1501 gattaataaa tatcctcaaa tacaaaaaaa tatgaaatca gagttcattg cttattatga
     1561 taaagaaaaa aatagagaaa cagtaaaaaa ctatgcttgg aaccttcaaa aatctataaa
     1621 tgacgttatg caatcatatc ctagcacaaa atttgtacag ttttataaag atgatatttc
     1681 cccgtcaatg gtagatggaa atggccgttt aaaatcggat actaatgtaa tttccaacga
     1741 gggaggaaag tatgatgaga acaaataatc atataaccga tttagatgct attgaattaa
     1801 gcagtgcttg gaattacaat tcgaaattgg tgactggcaa aaatgcacta ctatcagata
     1861 gccttttaag agatattaaa gtattaagtt ctaatgctaa aggtgacgca gacatgttag
     1921 aaactgaact tcatgctgtt ataaacgcaa tgtctcaaaa caaaggtaaa tctgttgatg
     1981 cacttaaaaa tagtaccgct tctctaaaaa acaatctaac caaattaaaa gagcaactcg
     2041 ataaaaccac acattaaatt catatatttt gagtgaatag aattatattt ccatagcaaa
     2101 tattcctgaa ctacacatgt tacataagag attcattttt gaagaaacgg gtaaaatgat
     2161 aaagtaatta tctttcacaa aaatccaaat attgggagga attaaatatg attttacgtt
     2221 acgtgaccaa tttaaaactt gcgaaagagc tataccaagc cagtaaacct aaattgcaag
     2281 aagataaggg tatgattgaa ctgtttgagc atacttttgg cttgcaaaaa gaacttgtaa
     2341 aatacgtcgg cattgcagaa gcaactactg ctgctttata ctcagctagc tttatcaata
     2401 aaaacatttc tcgcattgct tcgctatcaa caataggtat tttaagtgta gctacttaca
     2461 aacatttcga agctggccaa ggtaaaaaag gtgcacaaca tgcattagat ttattaggtc
     2521 ttgctacttt aagtttacta gatacatttt cttgtaataa aaaatagtct tcattttaga
     2581 agcacatact ttagcacctg tgtacttttg attcaattca taaacggtac accgctatag
     2641 catgtgtttt gttatttttc aggtctatct attgtaaata atcctcctaa tttagcataa
     2701 tcatttccgg ctaaacgaga aacagcctgt aatgcgtgtg gattgatttt aaaatcaccc
     2761 tcataaatac tgtcatcaat atgataagtt acaatttcgc ctattattaa gtcagcccca
     2821 tctaatacat ctccaagcaa tatcatttgc gatagtttac attcgaatct cattttcgcg
     2881 tctttaattc ctggcgtctt aatcgttgta gatgttaaaa gtgataattt tgtacgactc
     2941 aactcactgt ctccatatgc taacggcgct gcagtctcat taatatcttg aacattatct
     3001 tcgtctgtaa tatgcacaac aaagtctcca gtccgttcta tattcaatgc agtatctttt
     3061 ctcttacctc ctgcacgttg aactgcaata gcaatcattg gcggatggtt attaacaata
     3121 ttaaaaaagc taaatggtgc tgcatttact gatgcatctt gatttaatgt tgtaacaaat
     3181 gctataggtc gtggaataat tgaaccaatt aataatttat agttttctct agcagttaat
     3241 gattgtgcat caaacgtata cataatacct acctcttttc taagtatatc taggtatttc
     3301 tccgattttg gttaatttaa acatctattc tcctctgaaa atcacttgta tttatttagc
     3361 aaatcttttg aaatatgaca catatgcata tcttctggat atttttctaa atgttgctga
     3421 tgttcttcag cacttttaat gtagttagac agcggtaaga cttccactgc aatttgatct
     3481 ctgtctttac gtcgttcaat gaactgacgc gcttcaatta agtggtcatc tacacaacta
     3541 tataaacccg ttcgatactt ttctccaata tcatttcctt gttgattcac actgtaagga
     3601 tcaatgattt caaataaata attcataatg tctgtaattg ttaacatacg atcatcgaaa
     3661 tgaagtttga cacattcagc ataaccatca tacggaccgt ctaatttaga gcttcttcca
     3721 tttgctcttc ctgcttctgt atgtataatt ccaggtattg ttgcaaaaaa tgtttcaaca
     3781 ccccataaac atcctcctgc tacataaaca actgccatat ttacacctca tcatcctttt
     3841 ttatattttt aacaaggtta taccatttaa taccgccatg acatgattct gatacacctt
     3901 cattacgata cccatatttt tcataaaaag aaattaatga ttctcgacat gttaacgtta
     3961 caccatgtcg atgttgattc ttagcaagat tttcaaaata gtttagtaag cgacctgcaa
     4021 taccctgacc ttgataattt ggtgctacaa caagacctaa cacactaata tagccacctt
     4081 cactattatt tgtggagaca tttttaaata aatcatcgct aatgtaacgc tcttttatga
     4141 ctggaccatt gatgtatcca attacttcat cattcttttc agctacaata aaagtttctt
     4201 gaatttgttc aattctcaat ttcaatgcct ctggtgttgc agcttcttcc ggtgaaaaac
     4261 caatattttc aagtgcaata atttgatcta agtcctgcat ggttactttt cttaatacca
     4321 taaaaacact tccttagccc tatatatcaa aagtaatacc ttcatcttta tattcattat
     4381 tttcaagcta ccttcactat aaaaaacgag tctttttatt aaaagccatt cgccttaatc
     4441 aattagacaa gttgttttat acgaattagc aaataatcat gattataatt cattttaagc
     4501 atactcaaaa agactggtac atgtatacca gtctaattcg aaaaaatata tttagttaaa
     4561 accatttcaa aaacgacttc acttttcctg gcttagtcgg tctgtctaaa taaacaaaat
     4621 tatcattgat tactgaactg gcatttcgaa gtaatgcttc aatgtcattc gaatatttct
     4681 tcaatttatg attgtgaaat aattcttgca tcaaaaatgg tctttggtca catgaatgtg
     4741 catctgaagc tacaaaatga gccaaattac attctataaa ttgtaatgat aacttttgaa
     4801 tgttttttcc aaatccacca actaaagaac tcgatgttaa ttgactcaat gccccatttg
     4861 caacaagttc atataatatt tctggatttt tggcgatact tctatttctt tcaggatgtg
     4921 caatgattgg tatgtaacct ctcgattgta tttcaaaaaa caattgtttt gtataatgtg
     4981 gtacttcgcc cgttggaaat tcaattaata aatatttcga acgattaata ccttgaatac
     5041 taccattatc taagccttgc agaatcgaat ctgtaattct aatttcttgc ccgggaaata
     5101 atttaatatc caatgcttga acttctggat gcgttcttaa ctccgccaat ttcacaagca
     5161 cttgttgaaa tgtattatca tatctcggat gcaaatgatg aggtgtcgct acaatacttg
     5221 ttacaccttc atccttagct tgctttaata gtgcaatact cttttcaatt gttttaggac
     5281 catcatctat atcaactaat atatggttat gaatatcaat catgattcat cagtcccata
     5341 atatgcatag taactagcac ttttatcttt aggcattcta tttaagacta cacctaataa
     5401 tttagcacct gttgcttcaa taagttcttt tcctttttta acttcatctt tattattatt
     5461 ttccgaatta actacgtaga caacattgcc ggtaaacttt gaaaataatt gcgcatctgt
     5521 aactgtgttc actggcggcg tatcgataat aacaaagtta taattcatca ataatgtgtc
     5581 atacaaattt gcaaatgctc ttgatgtgat taactctgat gggtttggcg gaatcggtcc
     5641 agacgtcagg acgtctaaat cttcaatttc agttgagata atactgtctt gataagttga
     5701 ccaatttagc aataaacttg ataggccttc attgtttggt aaattaaaaa tatagtgctg
     5761 cgtaggttta cgcatatccc catctacgat taatgtttta taacctgctt gtgcatatgc
     5821 aactgctaaa tttgctgcaa ttgtagactt acctgcgcct ggtgcctctg aagtgattac
     5881 aatgctttga actgcactgt caggatttgc aaacataata tttgatctta tgcctcgaaa
     5941 tttctcgcta ataggtgact ttggttgttc atggacaatt aaacttgatg tacttcttcg
     6001 tgtattcgtc atggtaattc ctcgtaaatt aaaatttttg tattgaacct aaaataggta
     6061 atcctagttg cgattcaaca tcttcttccg tcttaatacg cttatctaat agttctttta
     6121 agaaaataat caatattgct aaaacaatac caacaatgat gctgatcact aagttgacag
     6181 agactatcgg agatactttt acagcattat catgcgctga ggaaagtatc gtaacattat
     6241 caacactcat aattttaggc atgtcatgag caaaaacttt agatatttta ttaacaattt
     6301 tgtcagattc agatttattc ccagttgtaa ctgatacagt aataatttga gagtttgttt
     6361 gattggttac ttttaaaaac gaattcaact cagctgttga atactggcca tcaaactctc
     6421 tagatacttt atctagaatt cttggacttt tgataatttc cgtatatgta ttaacagact
     6481 gcaaactact ttgaacattt tggaaagcta aatcacttga ggactttttc atgttcacta
     6541 atatttgagt agaagcagta tatttgtcag gcataacaaa aaaggttaat gccgcactta
     6601 ctacaagaca tattgccggt aaaataagca ataatttaat attcttcttt agaatattta
     6661 atagttttac taaatcaaac ttttctttca tggtttcctc cacataatca atcattgtat
     6721 tcattatgta tgttttataa atcggacaat tgtatctagt ttaacgacca caaaacatac
     6781 acaactacat tttctctaat tatttatata aatattttat cgtttaaaat tatatcatga
     6841 ttcttgggca ttatgtat
//
LOCUS       DAHHOJ010000120         6530 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.120, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000120 DAHHOJ010000000
VERSION     DAHHOJ010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6530
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.120"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..453
                     /locus_tag="NBH32_002286"
     CDS             <1..453
                     /locus_tag="NBH32_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridine nucleotide-disulfide oxidoreductase"
                     /protein_id="HCD1515859.1"
                     /translation="TAELIIELIENKSLILKKDLEDVKHDGKLYYFSYKNQESVDIYN
                     VVINATGAKSHLNELDQDDQLIKNLENRQIVQAHPMGGIQIIPETNQVISPRFGTLTN
                     MIAIGQMTNGVNKLRNGVKMIVEQVAHTVSQLYDALESNEQQQRSDNQ"
     regulatory      672..807
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            934..1977
                     /gene="ribD"
                     /locus_tag="NBH32_002287"
     CDS             934..1977
                     /gene="ribD"
                     /locus_tag="NBH32_002287"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCD1515860.1"
                     /translation="MSQFMDYAIQLANMVQGQTGVNPPVGAVVVKEGRIVGIGAHLRK
                     GDKHAEVQALDMAQQNAEGATIYITLEPCSHFGSTPPCVNKIIDCKITKVVYATKDNS
                     LDTHGDETLRAHGIEVECVDDERASQLYQDFFKAKAKQLPQITVKVSASLDGKQANDN
                     GQSQWITNKEVKQDVYKLRHRHDAVLTGRRTVELDDPQYTTRIQDGKNPIKVILSKSG
                     NIHFNQQIYQDESTPIWIYTENPNLTTKQTHIEIIFLESCDLTTILHNLYKRGVGTLL
                     VEAGPTTTSEFLQSNYIDEFILYYAPKLIGGSGNYQFYQTNDVIEIPDANQFEIVHSE
                     LLNQNVKLTLRKK"
     gene            1984..2616
                     /locus_tag="NBH32_002288"
     CDS             1984..2616
                     /locus_tag="NBH32_002288"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCD1515861.1"
                     /translation="MFTGIVEEIGVVKSVQIRQSVRTIEIEAHKITADMHIGDSISVN
                     GACLTVIDFNQTSFTVQVIKGTENKTYLADVKRQSEVNLERAMSGNGRFGGHFVLGHV
                     DELGTVSKINETANAKIITIQCSQHINNQLVKQGSITVDGVSLTVFDKHDNSFDIHLI
                     PETRRSTILSSKKLGDKVHLETDVLFKYVENILNKDKDQLSVDKLRAFGF"
     gene            2627..3808
                     /gene="ribB"
                     /locus_tag="NBH32_002289"
     CDS             2627..3808
                     /gene="ribB"
                     /locus_tag="NBH32_002289"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCD1515862.1"
                     /translation="MQFDNIDSALMALKNGETIIVVDDENRENEGDLVAVTEWMNDNT
                     INFMAKEARGLICAPVSKDIAQRLDLVQMVDDNSDIFGTQFTVSIDHVDTTTGISAYE
                     RTLTAKKLIDPSSEAKDFNRPGHLFPLVAQDKGVLARNGHTEAAVDLAKLTGAKPAGV
                     ICEIMNDDGTMAKGQDLQKFKEKHQLKMITIDDLIEYRKKLEPEIEFKAKVKMPTDFG
                     TFDMYGFKATYTDEEIVVLTKGAIRQHENVRLHSACLTGDIFHSQRCDCGAQLESSMK
                     YINEHGGMIIYLPQEGRGIGLLNKLRAYELIEQGYDTVTANLALGFDEDLRDYHIAAQ
                     ILKYFNIEHINLLSNNPSKFEGLKQYGIDIAERIEVIVPETVHNHDYMETKKIKMGHL
                     I"
     gene            3821..4285
                     /locus_tag="NBH32_002290"
     CDS             3821..4285
                     /locus_tag="NBH32_002290"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCD1515863.1"
                     /translation="MNFEGKLIGKDLKVAIVVSRFNDFITGRLLEGAKDTLIRHDVNE
                     DNIDVAFVPGAFEIPLVAKKLASSGNYDAVITLGCVIRGATSHYDYVCNEVAKGVSKV
                     NDQTNVPVIFGILTTESIEQAVERAGTKAGNKGAEAAVSAIEMANLLKSIKA"
     gene            complement(4407..5408)
                     /locus_tag="NBH32_002291"
     CDS             complement(4407..5408)
                     /locus_tag="NBH32_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase"
                     /protein_id="HCD1515864.1"
                     /translation="MALLKNFFIGLSNNSFLNNAAKKVGPRLGANKVVAGNTIPELIN
                     TIEYLNDKNIAVTVDNLGEFVGTVEESNHAKEQILTIMDALHQHGVKAHMSVKLSQLG
                     AEFDLELAYQNLREILLKANTYNNMHINIDTEKYASLQQIVQVLDRLKGEFRNVGTVI
                     QAYLYDSHELVDKYQDLRLRLVKGAYKENESIAFQSKEDVDANYIKIIEQRLLNARNF
                     TSIATHDHRIINHVKQFMKENHIEKDRMEFQMLYGFRSELAEEIANEGYNFTIYVPYG
                     DDWFAYFMRRLAERPQNLSLAVKEFVKPAGLKRVGIIAALGATVMLGLSTIKKLCRK"
     gene            5520..5639
                     /locus_tag="NBH32_002292"
     CDS             5520..5639
                     /locus_tag="NBH32_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515865.1"
                     /translation="MMLISFIYNNMDNNNTINRNLHSEFAIIMLLKINEKGAV"
     gene            5642..6469
                     /locus_tag="NBH32_002293"
     CDS             5642..6469
                     /locus_tag="NBH32_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1515866.1"
                     /translation="MWKWEAENDAKGVVVIAHNILEHTGRYAYVITMLRRNGYHVIMG
                     DLPGQGQISRANKGQIENFQTYHESLLDWLKIANEYKIPTYVLGVGLGGLILLNLLEK
                     VELPIEGMMLISPMLELQKNGKNRKDKLVSNIGKISKDARFNVGVEPKDLTRNLEIVE
                     ETVNDGLMLKKATYHWYNTINETMKDTMAHIHDIQPMPTLLMYGTKDLIVDTRAIDEF
                     KEKYQTPELYFKAWQGFYHEVHNEPERDEVMRYILTFLNNSVNTMGFIVEDDEIVEI"
BASE COUNT         2333 a          920 c         1225 g         2052 t
ORIGIN      
        1 actgctgaac taattattga attaatagaa aataagtctt taattttgaa aaaagattta
       61 gaagatgtga aacatgatgg caagttgtat tatttttcat acaaaaacca agaatcagta
      121 gacatatata atgttgtcat taacgcgaca ggtgcaaaaa gtcatttaaa tgaattggat
      181 caagatgatc aattaattaa aaatcttgaa aatagacaaa ttgtacaagc acacccaatg
      241 ggaggcatac aaatcatacc ggaaaccaat caagttatta gtccaaggtt tggaacatta
      301 acaaatatga ttgcgatagg tcaaatgaca aacggtgtta ataaactacg aaatggtgtg
      361 aaaatgatag tagaacaagt tgcgcataca gtatctcaat tatatgatgc tttagaatcg
      421 aatgagcaac aacagcgcag tgacaatcaa taatttgtaa ctagaagata ataaagagaa
      481 cgctctatag agacgaattg aaggtttgat tttaatgtct gttagtaaga atcatatcaa
      541 tgagatgcct atagtactca gattatatta aattaaaacc gtcattaatt gttttttcag
      601 aaaacatata gtatcatttt aaatgtagtt gacatactac gtactcaaat aatctataac
      661 aatttcatat ataattcttt cggggcaggg tgaaattccc aaccggcagt aaataaagcc
      721 tgcgacctgc taatatgttt catattagtg gctgatctag tgagattcta gagccgacag
      781 ttaaagtctg gatgggagaa agaatgttaa ttatcgacaa agataatgta gcgtatttgt
      841 aaaaatgtgt acaaataggc ttatttaacg ataaattttt ctccttgcat cttaattcat
      901 gatgtgagga tttttgttta tagaggtgat catttgagtc aatttatgga ttatgcgatt
      961 caacttgcaa atatggtaca aggtcaaaca ggtgttaatc cacccgttgg cgctgttgta
     1021 gttaaagaag gtaggattgt tggtattggt gcacacttga gaaaaggtga caagcatgcg
     1081 gaggttcaag cacttgatat ggcacaacaa aatgctgaag gtgcgacgat ttatattacg
     1141 ttagagccat gtagtcattt tggttcaaca ccaccctgtg ttaacaaaat tattgattgt
     1201 aagataacta aagtagttta cgcaacaaaa gacaattcgt tagacacaca tggtgatgag
     1261 acgttacggg ctcacggtat tgaggttgaa tgcgttgatg atgaacgtgc atcacaatta
     1321 taccaagact tttttaaagc aaaagcaaag caactgccac aaattacagt gaaagtatct
     1381 gcaagtttag atggtaaaca agcgaatgat aatggacaaa gtcaatggat tactaacaaa
     1441 gaggttaaac aagatgtcta taagttaaga catcgacacg acgcagtgtt aactggaaga
     1501 cgtacagttg aattagatga tccacaatat actacacgta ttcaagatgg aaaaaaccct
     1561 ataaaagtaa tattgtctaa gtctgggaat attcatttta atcagcaaat ttatcaagat
     1621 gaatcaacac caatttggat atatactgaa aatccaaatt taacaaccaa gcaaacacat
     1681 attgaaatta ttttcttgga gtcttgtgat ttaacaacaa ttcttcacaa tttatataaa
     1741 agaggagttg gaactttgct agtcgaggca ggtccaacca ctacttcaga atttctccaa
     1801 tctaattata tagatgaatt tattctctat tatgccccga aattaattgg cggatctgga
     1861 aattatcaat tttatcaaac aaatgatgtg attgagatac cagatgcgaa ccaatttgaa
     1921 attgttcatt ccgagttatt aaatcaaaat gttaaattaa ctttacgaaa gaagtgatga
     1981 tgcatgttta ctggcatcgt tgaagaaata ggtgtcgtta aaagtgttca aattcgtcaa
     2041 tcagtcagga cgattgaaat tgaagcacat aagattacgg cagatatgca tattggtgat
     2101 tccatcagtg ttaatggtgc atgtttaaca gtgattgatt ttaatcagac atcttttact
     2161 gttcaagtaa ttaaaggcac tgaaaataaa acctatttag cagatgttaa gcgacaatca
     2221 gaagtaaatt tagagcgtgc catgagtggt aacggtaggt ttggtggaca ttttgtcctc
     2281 ggtcatgtag atgaactagg aacagtttca aaaataaatg aaacagccaa tgccaaaatt
     2341 attacgattc aatgtagcca acatattaat aatcagttag ttaagcaagg gtctattact
     2401 gtggatggtg ttagtctaac ggtatttgat aagcatgata acagttttga cattcatctt
     2461 attccagaaa cgaggcgttc aacgatttta tcatccaaaa aattaggaga taaagtacat
     2521 ttagaaacag acgttttgtt taaatatgtt gaaaatattt taaataaaga taaagaccaa
     2581 ttatctgtag ataaattaag agcatttggt ttttaggagg ggtagcatgc aattcgataa
     2641 tattgacagt gctttaatgg ctttaaaaaa tggagaaaca attattgtag tagatgatga
     2701 gaatcgtgaa aatgaaggtg atttagtagc ggttactgaa tggatgaacg ataataccat
     2761 taattttatg gcgaaagaag caaggggatt aatatgcgca ccagtgtcta aagatattgc
     2821 acaacgtttg gatttggtac aaatggttga tgataactcc gacatctttg gtacgcaatt
     2881 tacagtgagt attgatcatg tagatacaac aacaggaatt agtgcttatg aacgtacatt
     2941 gactgccaaa aagctcattg atcctagtag tgaagctaaa gattttaatc gtcctggtca
     3001 tttatttcca ttagtagcac aagataaagg cgtattagct agaaatggac acacagaagc
     3061 ggctgttgat ttagctaaac ttactggtgc caagcccgct ggtgtcattt gtgagattat
     3121 gaatgatgac ggcacgatgg cgaaaggaca agatttacaa aagtttaaag aaaaacatca
     3181 attaaagatg attacgattg atgatttaat tgaatatcgt aaaaaattag aaccagaaat
     3241 tgaatttaag gcaaaagtga aaatgcctac agatttcgga acatttgata tgtatggttt
     3301 taaagcgaca tacacagatg aagagatagt tgtactgaca aaaggtgcaa ttcgacaaca
     3361 tgaaaatgta cgcttacatt ctgcgtgcct tacaggcgat attttccata gtcaacgttg
     3421 tgattgtggt gctcaacttg aatcgtctat gaagtatatc aatgaacatg gtggcatgat
     3481 tatttatcta cctcaagaag gtcgtggcat aggattgtta aacaaattac gcgcatatga
     3541 attaattgag caaggatatg atacagtaac tgcaaattta gcattaggtt ttgatgaaga
     3601 tttacgagat tatcatattg ctgcacagat tttaaaatat tttaacatcg aacatatcaa
     3661 tttattaagt aataatccaa gtaaatttga gggattaaaa caatatggca ttgatattgc
     3721 agaaagaatt gaagttatcg taccagaaac ggtacataat catgattata tggaaacgaa
     3781 aaaaataaaa atgggtcatt taatatagga ggactttaac atgaattttg aaggtaaatt
     3841 aattggaaaa gatttgaaag ttgcaatcgt agttagtcga tttaatgatt ttatcactgg
     3901 aagattactt gaaggtgcaa aagatacttt gattcgacat gatgttaatg aagacaatat
     3961 tgatgtagca tttgttcctg gtgcgtttga aattccttta gtagctaaga aattagcctc
     4021 atcaggaaat tatgatgcag taattacatt aggatgcgta attcgcggtg ctacgtctca
     4081 ttatgattat gtttgtaatg aagtcgcgaa aggtgtttct aaagtaaatg atcaaactaa
     4141 tgtaccagtc atatttggta ttttaacgac tgaaagtatt gaacaagctg tggaaagagc
     4201 aggtacgaaa gctggcaata aaggtgccga agcagcagta agtgcaattg aaatggctaa
     4261 tttattaaaa tctataaaag catagagcgc tgttgtgagt tcataatcaa agatttataa
     4321 atgattcaga actgccaata taacatttag gagcctagaa cattgattat tgttccagac
     4381 tcctaaatta ttgtttatgc catgctctat ttacggcata attttttaat tgtacttaaa
     4441 cctaacataa ctgtagctcc taaagctgca attatgccaa cacgttttaa gccagcaggt
     4501 ttcacaaatt cttttacagc aagagatagg ttttgtgggc gttctgctaa tcttctcata
     4561 aaatacgcaa accaatcatc gccataaggt acataaatag tgaaattata gccttcattt
     4621 gcgatttctt ctgctaactc tgatctaaaa ccatagagca tttggaattc catacgatct
     4681 ttttcaatgt gattttcttt cataaattgt tttacatgat taatgatgcg atggtcatgt
     4741 gttgcaattg aagtgaaatt gcgtgcgttt aacaaacgtt gttcaattat tttgatgtaa
     4801 tttgcatcta cgtcttcctt agattgaaat gcaattgatt cgttttcttt atatgcacct
     4861 ttaaccaaac gtaatcgtaa atcttggtac ttatcaacta attcatggct atcgtataaa
     4921 tatgcttgaa ttacagtacc aacatttcta aattcgcctt ttaagcgatc taaaacttga
     4981 acaatttgtt gcaggctagc atatttttca gtatcaatat ttatatgcat attgttgtaa
     5041 gtatttgctt taagtaaaat ctctcttaaa ttttggtaag ctaattctaa gtcgaattct
     5101 gcacctaact gactcaattt aacagacata tgtgccttta cgccatgttg atgaagcgcg
     5161 tccataattg ttaaaatttg ttctttagca tgattacttt cttcaactgt accgacaaat
     5221 tcccctaaat tgtctaccgt aacagcgata ttcttgtcat ttaagtattc gattgtatta
     5281 attaactctg gaattgtatt tccggcaacg actttattgg cgcccaaacg tgggcccact
     5341 ttttttgctg cgttgtttaa aaaactatta ttagataatc cgataaaaaa attctttaat
     5401 agtgccattg ctactcctcc aaacccttgt gtttacaata agtttagcat gaaaaaaata
     5461 ttcgagaaag cgttttcatc aaaaacaatc aaaactaatt atgtataatt acactattat
     5521 tgatgttgat ttcatttata tataataata tggacaacaa taatactata aatagaaatc
     5581 tacatagtga atttgctata attatgttat tgaagattaa tgaaaaaggg gcagtataat
     5641 aatgtggaag tgggaagctg aaaatgatgc gaaaggcgtc gttgttattg ctcataatat
     5701 attagaacat acgggaagat atgcatatgt tattacgatg ttaagaagaa atggctatca
     5761 tgtgattatg ggagatttgc ctggtcaagg tcaaatttca agagctaata aaggacaaat
     5821 tgaaaacttt caaacgtatc acgaaagttt gctagattgg ttaaagatag ctaatgaata
     5881 taaaattcct acttatgtat taggggtagg acttggcggt ttgattttat taaatttact
     5941 tgaaaaagta gaattgccaa tcgaggggat gatgttaatt tctcctatgc tagaacttca
     6001 aaagaatgga aaaaatcgta aagataaact tgtttcaaat ataggtaaaa tttcaaaaga
     6061 cgcacgtttt aatgttggtg tagaaccaaa agatttaaca cgtaatttag aaattgttga
     6121 agaaacagtt aacgatggtt taatgttaaa aaaagcaacg tatcattggt ataatacaat
     6181 taatgagacg atgaaagata cgatggctca tattcatgac attcaaccaa tgccaacgct
     6241 attaatgtat ggaacgaagg atttaattgt tgatactagg gcaattgatg agtttaaaga
     6301 aaaatatcaa acacctgaat tatattttaa agcttggcaa ggtttttacc acgaggttca
     6361 caatgagcca gaacgcgatg aagtaatgcg ttatattctg actttcttaa ataatagcgt
     6421 caatacaatg ggctttattg ttgaagatga cgaaattgta gaaatttaat attctaaaaa
     6481 gatgagacat aaatccctaa aaaacagcag taagataatt ttcaattaga
//
LOCUS       DAHHOJ010000121         6490 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.121, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000121 DAHHOJ010000000
VERSION     DAHHOJ010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.121"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            101..2149
                     /gene="eap"
                     /locus_tag="NBH32_002294"
                     /gene_synonym="map"
     CDS             101..2149
                     /gene="eap"
                     /locus_tag="NBH32_002294"
                     /gene_synonym="map"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000669709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular adherence protein Eap/Map"
                     /protein_id="HCD1515867.1"
                     /translation="MKFKSLITTTLALGVLASTGANFNTNEASAAAKPLDKSSSTLHH
                     GHSKIQVPYTITVNGTSQNILSSLTFNKNQQISYKDLENKVKSVLYFNRGISDIDLRL
                     SKQAEYTVHFKNGTKKVVDLKSGIYTADLINTSDIKAISVNVDTKKQPKDKAKANVQV
                     PYTITVNGTSQNILSSLTFNKNQNVSYKDLEDKVKSVLESNRGITDVDLRLSKQAKFT
                     VNFKNGTKKVIDLKAGIYTANLINSSDIKSININVDTKKHIENKAKRNYQVPYSINLN
                     GTSTNILSNLSFSNKPWTNYKNLTSQIKSVLKHDRGISEQDLKYAKKAYYTVYFKNGG
                     KRVVHFNSKTYSANLVHAKDVKKIEVTVKTASKAKAERYVPYSIAVNGTSTPNLSDLK
                     FTGDPRVGYKDITKKVKSVLKHDRGIGERELKYAKKATYTVHFKNGKKKVINLNSKIS
                     QLNLLYVQDIKKIDIDVKTGSKAKADSYVPYTIAVNGTSTHILSKLKISNKQLIGYQD
                     LNEKVKSVLKHDRGINDIELKFAKQAKYTIHFKNGKTQVVDLKSDIFTRNLFSAKDIK
                     KIDIDVKQHTKSKKINKSNNVKFPVTINKFENIVSNEFVFYNASKITINDLSTKLKSA
                     MANDQGITKHDIGLAERAVYKVYFKNGSSKYVDLKTEYKDRKVFKATDIKKVDIELKF
                     "
     gene            complement(2428..3714)
                     /locus_tag="NBH32_002295"
     CDS             complement(2428..3714)
                     /locus_tag="NBH32_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1515868.1"
                     /translation="MNPLAQSLNEQIQQSNATAFAMLSDLGQNMFYPKGILSQSAEAK
                     STTYNATIGMATNKDGKMFASSLDAMFNDLTPDEIFPYAPPQGIEELRDLWQQKMLRD
                     NPELSIDNMSRPIVTNALTHGLSLVGDLFVNQGDTILLPEHNWGNYKLVFNTRKGANL
                     QTYPIFDKDGHYTTDSLVEALQSYNKDKVIMILNYPNNPTGYTPTHKEVTTIVDAIKA
                     LADKGTKVIAVVDDAYYGLFYEDVYTQSLFTALTNLHSNAILPVRLDGATKEFFAWGF
                     RVGFMTFGTSDQTTKEVLEAKVKGLIRSNISSGPLPTQSAVKHVLKNNKQFDHEIEQN
                     IQTLKERYEVTKKVVYADQYRSYWQAYDFNSGYFMAIKVHDVDPEELRKHLIEKYSIG
                     VIALNATDIRIAFSCVEKDDIPHVFDSIAKAIDDLR"
     gene            3914..4012
                     /locus_tag="NBH32_002296"
     CDS             3914..4012
                     /locus_tag="NBH32_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515869.1"
                     /translation="MMWWQEGMMTLITGGLLIIFRLWLELKWKNKK"
     gene            4255..4413
                     /locus_tag="NBH32_002297"
     CDS             4255..4413
                     /locus_tag="NBH32_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515870.1"
                     /translation="MKHLTKIFVVLAIILFIIGYYLQATNHESQGIKLLLAAIMFMIC
                     AFISRSND"
     gene            4690..5070
                     /locus_tag="NBH32_002298"
     CDS             4690..5070
                     /locus_tag="NBH32_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1515871.1"
                     /translation="MKIILKNNSDFPIYEQIKQQVKQNILKGHVVPGEHLPSMRELAK
                     DLQVSLITTKRAYEDLEKDGFVTTIRGKGTFVKEQDSSILKEKQFFTIENLVKELVNE
                     AQAIEMSLEELQDILTFIYEEESS"
     gene            5067..5963
                     /gene="pmtA"
                     /locus_tag="NBH32_002299"
     CDS             5067..5963
                     /gene="pmtA"
                     /locus_tag="NBH32_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     ATP-binding protein PmtA"
                     /protein_id="HCD1515872.1"
                     /translation="MNAIELSNVNYSSDQFNLKNISFKVPQGFVTGFIGRNGAGKTTI
                     IRLIMDLYQPQTGVIRVLEEDMALNPIELKNRIGFVYAENYFNERWTTKQLEKMIAPF
                     YSNWDHQVFQFYLEKFDLPINKSIKTFSTGMKMKLSLAVAFSHHAELYIFDEPTSGLD
                     PLARNELLEIIQQELIDENKTIFMSTHIISDLEKIADYIIHLSDGEVILNGSKEQLLQ
                     RYQVVSGALEDLDEELAALLIYEEHKRTGFIGLTEHAQVFKEILGHKVNITTPSIENL
                     MVYLEKRKPKYHENIKLMEEGF"
     gene            5964..>6490
                     /gene="pmtB"
                     /locus_tag="NBH32_002300"
     CDS             5964..>6490
                     /gene="pmtB"
                     /locus_tag="NBH32_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenol-soluble modulin export ABC transporter
                     permease subunit PmtB"
                     /protein_id="HCD1515873.1"
                     /translation="MKALLIRNFKLRRYTLIIYVLLLTLYPFYIMLDSTKFFYLLQSF
                     ISPTILIIWILDAGHLFRLNRRLGGNDSYYFYMSLPVSKKQLLNANYITCIVLTLIGT
                     LVISLYAYEANVIEPNSIYFSTAYAFVISNFLSIPIAFSQFTELRRVKVPYGIYVFTI
                     IILVPFLFSIAIVLV"
BASE COUNT         2393 a          876 c         1074 g         2147 t
ORIGIN      
        1 agataattta ttattaatat tcagttaatt caaaaaacgg agcagacctg gctataataa
       61 agttgcaatc acgaattaca taaaaaagga gagataattt atgaaattta agtcattgat
      121 tacaacaaca ttagcattag gtgttttagc atcaacagga gcaaacttta atactaacga
      181 agcatctgcc gcagctaagc cattagataa atcatcaagt acattacacc atggacattc
      241 taaaatccag gttccatata caatcacagt gaacggcaca agccaaaaca ttttatcaag
      301 cttaacattt aataagaatc aacaaattag ttataaagat ttagagaata aagttaaatc
      361 agttttatac tttaatagag gtattagtga tatcgattta agactttcaa agcaagcgga
      421 atatacggtt cattttaaaa atggaacaaa aaaagttgtc gatttgaaat caggtatcta
      481 cacagctgac ttaatcaata caagtgacat taaagctatc agtgttaacg tagatactaa
      541 aaagcaacct aaagataaag ctaaagcaaa tgttcaagtg ccgtatacaa tcacggttaa
      601 tggtacaagc caaaacattt tatcaagctt aacatttaat aagaatcaaa atgttagtta
      661 caaagattta gaggataaag ttaaatcagt tttagagtcc aatagaggta taactgatgt
      721 agatttaaga ctttcaaaac aagcgaaatt tacagttaat tttaaaaatg gaacgaagaa
      781 agttatagat ttgaaagcag gtatttacac agcgaattta ataaattcaa gtgatattaa
      841 aagtatcaat attaacgtag atacaaaaaa acatatcgaa aataaagcta aaagaaacta
      901 tcaagttcca tattcaatta atttaaatgg tacatctaca aacattttat cgaacctttc
      961 attttcaaat aaaccttgga caaattacaa aaatttaact agtcaaataa aatcagtact
     1021 gaagcatgat agaggtatta gtgaacaaga tttaaaatat gctaagaaag cttattatac
     1081 tgtttatttt aaaaatggtg gtaagagagt ggtacatttt aattcaaaaa cttactcagc
     1141 gaatctagtt catgcgaaag atgttaagaa aattgaagtt acagttaaaa cagcatctaa
     1201 agcaaaagcg gagcgttatg taccatactc aattgcagta aatggtacat caacaccaaa
     1261 tttatcagat ttaaaattta caggtgaccc acgtgtaggc tacaaagata tcactaaaaa
     1321 agttaaatca gtattgaagc atgatagagg tatcggggaa cgtgaattaa aatatgcaaa
     1381 aaaagcaact tacacagtac attttaaaaa tggaaagaaa aaagtgatta atttaaactc
     1441 aaaaattagc caactgaatc tactttatgt tcaagatatt aaaaagatag atatcgatgt
     1501 taaaacagga tcaaaagcga aagcagatag ctatgtacca tatacaattg cagtaaatgg
     1561 tacatcaaca catattttat caaaactaaa aatttcaaat aaacaattaa taggttatca
     1621 agatttaaat gaaaaagtta aatcagtttt aaaacatgat agaggtatca atgatattga
     1681 attgaaattt gcgaaacaag cgaagtatac tatacacttt aaaaatggaa agacacaagt
     1741 tgttgacctt aaatcagata tctttacaag aaacttattc agtgccaaag atattaaaaa
     1801 gatagatatt gatgtaaaac aacacactaa atcaaaaaaa ataaataaat ctaataatgt
     1861 gaaattccca gtaacaataa ataaatttga aaacatagtt tcaaatgaat ttgtgttcta
     1921 taatgcaagc aaaattacaa ttaatgattt aagtacaaaa cttaaatcag caatggcaaa
     1981 tgatcaaggg attactaaac atgacatagg acttgctgaa cgcgcggtgt ataaagtgta
     2041 ttttaaaaat ggttcgtcaa aatatgtaga cttaaaaact gagtataaag atagaaaagt
     2101 atttaaagca actgacatta aaaaggtaga tattgaactt aaattctaat ctttaatttt
     2161 ataataaggc atctcacaat cgtggggtgc cttttacatt tgtagagatg tggtgcttga
     2221 atgcttgaag tgatttgcca aaagttatat aaatttgcac caaataaatt aagttttgtt
     2281 aggagagatg tataaaacta attaggtata gcgtttgaca agttgctgaa taaaatatat
     2341 cttatgatgc tttaaaagaa ggaataattt taaaaataaa aaaccaataa tccgagtcat
     2401 attcatcaga ttattggttg aagttaatta tcttaagtca tctatcgctt tagcaattga
     2461 atcgaagaca tgtgggatat cgtctttttc tacacagcta aatgcaatac gaatatctgt
     2521 tgcatttagt gcaataacac caattgaata tttttcaatt aaatgtttac gaagttcttc
     2581 agggtcaaca tcatgaactt ttattgccat aaagtatcca gagttaaagt catatgcttg
     2641 ccaatatgat cggtattgat cagcatagac tactttctta gtgacttcat agcgttcttt
     2701 taatgtttga atattttgtt cgatttcgtg atcgaattgc ttattatttt tcagtacatg
     2761 tttaacagcg ctttgtgttg gaagcggtcc gctggaaata ttgcttctaa taagaccttt
     2821 tactttggct tctaatactt ctttagttgt ttgatcagac gttccaaatg tcataaatcc
     2881 aacacggaat ccccatgcga aaaattcttt tgttgcacca tctaaacgaa caggtaatat
     2941 tgcatttgaa tgtaaattag ttaatgcagt aaataatgat tgagtataca catcttcata
     3001 gaataaacca tagtatgcat catccacaac agctataact tttgtacctt tatcagctaa
     3061 tgctttaatt gcatcgacga tggtagtcac ttctttatgc gtaggtgtgt aacctgtcgg
     3121 attattagga taatttaaaa tcataatgac tttatcttta ttgtatgatt gtaaagcttc
     3181 tactagtgaa tcagtagtat aatgtccgtc tttatcaaag ataggatatg tttgaaggtt
     3241 tgcacctttt ctcgtattga aaacaagttt gtaattaccc caattatgct ctggtaacaa
     3301 gatagtgtca ccttgattta caaataaatc gccaactaga gataaaccat gtgttaatgc
     3361 attcgtaaca attggtcgtg acatgttgtc gattgatagc tctggattgt cacgcaacat
     3421 tttttgttgc cataaatcac gtaattcttc gatgccttgt ggtggcgcat aagggaatat
     3481 ttcatctgga gttaaatcat taaacattgc atctaaagat gatgcaaaca tttttccgtc
     3541 tttgtttgtc gccataccta tagttgcatt atatgttgta ctctttgctt cagcagattg
     3601 agataaaatg ccttttggat aaaacatatt ttgacctaag tcagataaca ttgcaaaggc
     3661 agttgcattt gactgttgaa tttgttcatt taagctttgg gctaaaggat tcattattgc
     3721 ttaacctctc tttaaaaaaa ttgtatgcct atatattatc ttcaattata ccaaatataa
     3781 agtgttgttg gtgaattaac gaataatatg taaaatctgc taccaaaata cgaacatatg
     3841 ttctgttttt gtgttagaat atgtataggt catccttcag tatgatactt tagtcgaacg
     3901 gaggtgtagt gtaatgatgt ggtggcaaga agggatgatg acacttatta caggtggctt
     3961 actaattatt tttcgacttt ggttagaact gaaatggaaa aataaaaaat gatcctacac
     4021 acataaataa ccccaagtct gctggtacca gacttggggt tgtgtagtat aattctgtgg
     4081 atacacttaa ataatatcat tgaacacaat atatcgcaat caataatcat tttgattgaa
     4141 tggttttgtt gcgctatttt agttgaaagt gattagtacc ttgaactttt gaaattcaat
     4201 aacttattaa aaaaatttat atttgttata atgtcgatgt tgaggtgaaa taaattgaaa
     4261 catctaacaa aaatatttgt agtacttgca attattttat ttattattgg ttattattta
     4321 caagcaacta atcatgaaag tcaaggtata aaattattat tagcagcgat tatgtttatg
     4381 atttgtgctt ttataagtag aagtaatgat tgacgtaaaa atagtaaata ggattgactg
     4441 tttaaaacga ttgtttatgt tgacgctacg gttgtcacgt agagattaaa gacattcgtt
     4501 ttagcagtct tttttattga agtagacaat gcaagattaa atggtagtgt catttcattt
     4561 tattcaactg ctagtaatac aatactcagt gttatttctg aagactggtt gataatgaga
     4621 catgcatatt tacaaaactg tgtatattgt gtatattgta tatatacaga aggcgatagg
     4681 gggacgttga tgaaaataat tttaaaaaac aatagtgatt ttccgattta tgaacagatt
     4741 aagcaacaag taaaacaaaa tattttaaag ggacatgttg ttcctggaga gcatttgccg
     4801 tcaatgagag aacttgccaa agatcttcaa gtaagtttga ttactaccaa acgtgcttat
     4861 gaagatttag agaaagacgg ttttgttaca acaattagag gaaaagggac ctttgttaag
     4921 gagcaagata gttctatttt aaaagagaaa caatttttta ccattgaaaa tttggttaaa
     4981 gaattggtta atgaagcgca agcaatcgaa atgtcacttg aggaacttca agatatttta
     5041 acgttcatct atgaggagga atcatcatga atgccataga attaagtaat gttaattatt
     5101 caagtgatca gtttaattta aagaatatct ctttcaaagt tcctcaaggt ttcgtcactg
     5161 gttttattgg tagaaatggc gctggtaaaa ccacaataat aaggttaatt atggatttgt
     5221 atcaaccaca aactggtgtt attagagtat tagaagagga tatggctctc aatccgatag
     5281 agttaaagaa tagaattggg tttgtttacg ctgaaaatta ttttaatgag agatggacta
     5341 cgaagcaact tgaaaaaatg attgcccctt tttatagtaa ctgggatcat caagtatttc
     5401 agttttatct tgaaaagttc gatttaccaa tcaataaatc aataaaaaca ttttcaactg
     5461 gcatgaaaat gaaattatca ttagcagttg cattttcaca tcatgctgaa ttgtatattt
     5521 ttgatgagcc tacttcaggt cttgatccct tagctagaaa tgagttattg gagataattc
     5581 agcaagaatt aattgatgaa aataaaacaa tattcatgtc gacacacatt atctcagatt
     5641 tagaaaaaat agctgattat atcattcact taagtgacgg tgaagttatc ttaaatggtt
     5701 caaaagaaca actgttacaa agatatcaag ttgtgagtgg agcccttgaa gatttagatg
     5761 aagaattggc tgctttgctg atttacgaag aacataagcg tacgggattt ataggattaa
     5821 cagaacatgc acaagtattt aaagaaattt taggtcataa agtgaatatc actacacctt
     5881 ctattgagaa cttaatggtt tatcttgaaa agagaaaacc taaataccat gaaaacatca
     5941 aattgatgga agaaggtttt taaatgaagg cattgttaat taggaatttt aaattacgac
     6001 gatatacact tatcatctat gttctattat taaccttata tcctttttat ataatgcttg
     6061 actcaacaaa gttcttttac ctattgcaat cttttataag tccaacaata ttgattattt
     6121 ggattttaga tgctggtcac ttatttagac tcaatcgtag attaggtggt aatgattcat
     6181 attattttta tatgagttta ccagtgtcaa aaaaacaatt gcttaatgcc aactatatta
     6241 catgcattgt tttaacatta attggaacgc ttgttattag tttatatgct tatgaagcaa
     6301 atgtgattga accaaattca atctattttt caactgcata tgcatttgtc atatctaact
     6361 tcttgtctat accaattgca tttagtcaat ttacagaatt gcgtagagtc aaagtgccat
     6421 atggtatata cgtgtttact attatcattt tagttccatt tttattttca attgcaatag
     6481 tgttggtgaa
//
LOCUS       DAHHOJ010000122         6490 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.122, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000122 DAHHOJ010000000
VERSION     DAHHOJ010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6490
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.122"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..1019)
                     /locus_tag="NBH32_002301"
     CDS             complement(<1..1019)
                     /locus_tag="NBH32_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HCD1515874.1"
                     /translation="MRVAIIGMGTAGVSVLRELVKHPKFNQLDIDLYDDKVNMGQGVP
                     FQNDSSELLINMPSKKMSLNLDDETEFWKWYKQQTDFNFDEPAYLPRFVFGHYMKSYL
                     SMFTKKYPNISTNYNKVQEIYTNSNIDETNLTYYICTTNSGQSWQAYDYVFLTCGTFA
                     YHDPYNLKGKKGYIATPYPTYNTLDEVNELDDIAIIGTGLASLDVVRYVAAHHPKLPI
                     TMTSRSAHLPGVRGTMIDVTFKYLTKDKLNDIKKHHFGNAPLDTLVSLFLKECAEYDI
                     DFKKLVHRRTGNHIADLKYDLARPTEMGIFQSMIEHLKENLNWIWNSLSIEDQHQFNQ
                     KYSKM"
     gene            1546..1860
                     /locus_tag="NBH32_002302"
     CDS             1546..1860
                     /locus_tag="NBH32_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCD1515875.1"
                     /translation="MTYKELATYLKVLSDSSRLEILDLLSCGELCACDLLEHFQFSQP
                     TLSYHMKALVKTNLVTTRKIGNKHLYQLNHNIFESVINNLSKVHTSNQRCICHNLKTG
                     EC"
     gene            1860..3152
                     /gene="arsB"
                     /locus_tag="NBH32_002303"
     CDS             1860..3152
                     /gene="arsB"
                     /locus_tag="NBH32_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite efflux transporter membrane subunit
                     ArsB"
                     /protein_id="HCD1515876.1"
                     /translation="MMTTLATLIFLVTLLFVLWQPKGLDIGFTALAGAIVAMITGVVS
                     LTDVFEVTGIVWNATLTFVSVILISLILDKIGFFEWSAIHMLHASKGNGLKMFVYIIL
                     LGAVVAAFFANDGSALILTPIVLAMVKNIGFSKRAIFPFIIASGFIADTTSLPLIVSN
                     LVNIISADYFNISFSQYLSRMIIPNLFSLLGSLLVLWLYFRKAIPKSFDANHIKKPKD
                     AINDLKLFKISWIVLVILLFGYLISEFTKIPVSIFTGIIAFIFLMLARKSNAVNIKQV
                     IKGAPWNIVLFSIGMYIVVFGLRNAGITLILAKILEYISNYGLFSTILGMGFISAFLS
                     SIMNNMPTVLIDAIAIGQSNVHGMLKEGLIYANVIGSDLGPKITPIGSLATLLWLHVL
                     TQKDVKISWGTYFKTGITITIPVLFITLLGLYLTLIIF"
     gene            3170..3565
                     /gene="arsC"
                     /locus_tag="NBH32_002304"
     CDS             3170..3565
                     /gene="arsC"
                     /locus_tag="NBH32_002304"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (thioredoxin)"
                     /protein_id="HCD1515877.1"
                     /translation="MTKKTIYFICTGNSCRSQMAEGWAKQILAKDWDVYSAGIETHGV
                     NPKAIEAMKEVGIDISNHTSDLIDNNIIKNSNLVVTLCSDADVNCPTLPTNVKKEHWG
                     FDDPAGKPWSEFQRVRDEIKVAIENFKSR"
     gene            complement(4195..5337)
                     /locus_tag="NBH32_002305"
     CDS             complement(4195..5337)
                     /locus_tag="NBH32_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000592652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515878.1"
                     /translation="MIKKSLIVPLYINIFLVFTAISLVVPSLPLIIKDLNIDGDAFGI
                     LIAVFSLFQMIFSPIVGFFSDRYAKKHFLSIGLLLYIVSEFLFGIGMGFYTLLSSRIL
                     GGISAALIQTSVIGIIGDISDEANRDKNFGTFSAITSLGFIIGPGIGALFATFHLRLP
                     YFIAMAIGIIMLIINLNLKIPHVQTLASKENKLATLSFKYLKLYLLPAMVILLLAFGL
                     AAIEELYPLYLVDKADFTSFHIALAIIGGSLLGALTQYLVYPKISRHLHEFSIIIISS
                     IYSIMVLCILLLLNSVIALVLISCVIFIGFDIIRPAITVYLSKIAKDKQGLAGSMNST
                     FTSLGTLIAPIIAGYFYVQNINAPVYIAILTIILTIIALIFLPKKE"
     gene            complement(5657..6199)
                     /locus_tag="NBH32_002306"
                     /pseudo
     CDS             complement(5657..6199)
                     /locus_tag="NBH32_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
BASE COUNT         2215 a         1098 c          945 g         2232 t
ORIGIN      
        1 atcatctttg agtatttttg attaaattga tgttgatctt caatacttaa gctattccat
       61 atccaattta aattctcttt caaatgttca atcatacttt ggaaaatgcc catttctgtt
      121 ggacgcgcta aatcatactt caaatctgca atatgatttc ctgtacgtct atgtactaat
      181 tttttaaaat caatgtcata ttcagcacat tcttttaaaa ataaagaaac taaagtatca
      241 agcggtgcat tgccgaaatg atgtttttta atgtcattta atttgtcttt agttaagtac
      301 ttgaatgtca cgtctatcat tgtacctctt acacctggta aatgagcaga acgactcgtc
      361 atagtaattg gtaatttcgg atgatgagca gcaacataac ggacaacatc taaactggca
      421 aggcctgtac caataatcgc aatatcgtcc agttcattta cttcgtctaa cgtattatat
      481 gttggataag gcgtagcgat atatcctttt ttacccttta agttatatgg atcatggtag
      541 gcaaatgtac cacatgttaa aaatacataa tcgtacgctt gccatgattg tcctgaattt
      601 gtagtacata tgtaataagt taaattcgtt tcatcgatat tagaatttgt ataaatctct
      661 tgaactttat tataattagt tgatatattt ggatattttt tcgtgaacat agataaataa
      721 gatttcatat aatgtccgaa tacaaatctc ggtaaatatg caggttcatc aaaattaaaa
      781 tcagtttgtt gtttatacca cttccaaaat tcagtctcat catctaaatt taaactcatc
      841 tttttcgaag gcatattaat tagcagctca gaactatcat tttgaaatgg tacgccctgt
      901 cccatattta ctttatcatc gtataaatct atatctaatt gattaaactt cgggtgctta
      961 actaactctc tcaatacact tacaccagca gttcccatgc ctattattgc tacacgcata
     1021 tatcatccat ccttttgtaa ttcaaaatat tatatttaaa ctattaccta taattatagc
     1081 aatttggtat ttccaataaa atttattaag gtaagctatt atttcacttt cacaaccatt
     1141 aaaccattta aaacttttca tttaatatca acctcagtgt ttgtgttaaa atatttttac
     1201 cgaggtgaat taaattgaaa tttatagcaa agatattaat ctcattagta attctattta
     1261 tgatttttag tcgtaaaaac aaagcatctt aatcaatacc cttgattaca aaataaattc
     1321 aactaaggtg attggccata cactcaagat atggcaaatc acctttttca ttacgtaaat
     1381 tcaaccatcc tttagcaaaa ttttaaccat acattttcac aagtaataat ttttaacaat
     1441 gctattagca tcacagtgac aaatgactaa cgaaacatta aagcaatcat acatagacat
     1501 tcatatatat agatgatagt ctatttaacg tctaggaggt atgttatgac gtataaagaa
     1561 ctagcaacat atttaaaagt tttgtcagat tcaagcagat tagaaatact agatttactt
     1621 tcttgtggag agttatgcgc ttgtgatttg ttagaacatt ttcaattctc tcaacctaca
     1681 cttagctatc atatgaaagc attagtaaaa accaacttag ttacgacacg aaaaatcgga
     1741 aataaacatt tatatcagct taatcataat atttttgagt ccgtaattaa taacttgtca
     1801 aaggttcata cctctaacca acgatgtatt tgtcataacc ttaagactgg tgaatgctaa
     1861 tgatgacaac tttagcgaca ctcatttttc tagtaacttt attatttgta ttatggcaac
     1921 ctaaaggcct agatattgga tttactgcat tagcaggtgc aattgttgca atgattactg
     1981 gtgttgtaag tttgactgat gtttttgaag taacaggtat agtctggaat gccactttaa
     2041 cttttgtctc agtcattctt atttcattaa tattagataa aattggattt tttgaatggt
     2101 cagctatcca tatgcttcat gcttcaaaag gcaatggttt aaaaatgttc gtttatatca
     2161 tattattggg tgccgtggtt gctgcatttt tcgcaaatga tggctcagcg ttaatcttaa
     2221 cgccaattgt attagcaatg gtaaaaaata taggttttag taagcgggca atattccctt
     2281 ttattattgc gagtggtttt atagctgata caacttcttt acctttaatc gtgagcaatt
     2341 tggtcaatat catttctgca gattacttta acattagttt ctctcagtat ctcagtagaa
     2401 tgattatccc taatttattc tcattattag gcagccttct agttttgtgg ttatatttta
     2461 gaaaagcgat tcctaaatca tttgatgcaa atcacataaa aaaacctaaa gatgccatta
     2521 atgatttaaa gctatttaaa atttcatgga ttgttctagt tatattactt ttcggctatc
     2581 taatcagtga atttactaaa attccggtat caattttcac tgggatcata gcttttatat
     2641 ttctaatgtt ggctcgtaaa tcaaatgctg taaatattaa gcaagtcatt aagggcgcac
     2701 cttggaatat agtattattt tcaattggta tgtatatcgt cgtattcggc ttaagaaatg
     2761 ctggcattac tttaatattg gctaaaatat tagaatatat ttccaattac ggtctattta
     2821 gcaccatttt gggaatgggc ttcatttcag cgtttttatc atcaataatg aataatatgc
     2881 ctacagtttt aatagatgcg attgctattg gtcaatcaaa tgtccatggc atgttaaaag
     2941 aaggcctaat ttatgcgaat gttatcggtt ctgatttagg tccaaaaatt acaccgatag
     3001 gctctttagc tacattactg tggttacatg tcttaacaca aaaagatgtt aagatttctt
     3061 ggggcacata ctttaaaact ggtatcacca ttacaattcc agtactattt atcacacttt
     3121 taggattata tctaacactt atcatatttt aagaaattag gagattgtta tgactaaaaa
     3181 aacaatttat tttatatgta caggcaactc atgtcgaagt caaatggctg aaggttgggc
     3241 taaacaaatc ttagcgaaag attgggatgt atattctgcg ggtatcgaaa cacacggtgt
     3301 taatcccaaa gcgatagaag ctatgaaaga agtaggcatt gatatatcaa atcatacatc
     3361 agatttaatc gataataata ttattaaaaa ttcaaattta gttgttacat tatgtagtga
     3421 tgcagacgta aattgcccta ctttaccaac aaatgttaaa aaggaacatt ggggatttga
     3481 tgatcctgca ggcaaaccat ggtcagagtt ccaacgtgtg agagatgaaa ttaaagtcgc
     3541 aattgaaaat tttaaatcac gatgagagtg ggacagaaat gataaaaaaa cacttatgat
     3601 ttattatgta gtggctcttt atcattagct ataactaatg tgtacttaaa aataggaata
     3661 catgagtaaa actcatgcat aagaaatact aatttctata gaagaagtaa ctcttcatcg
     3721 atgtcctacc ccaacttgca ttgcctgtag aaattggtgt tccaattcct ctatgttagg
     3781 gtcccgccaa cttgcattgt ttgtatacct actttttgtc aacttctatg ttgggtccca
     3841 cttgcattgg cggaattatc aatcaatatc tttctttaat aggcaatgtg tcaagctaaa
     3901 actgaaattt cttcaaaaaa attaaaaaaa gttgttagac agaaattgaa cagaataaca
     3961 gtggattttt taagtttaaa acaaaataaa aggaaagctt tgagaagctc tccgaaataa
     4021 atttacaaaa aaatacaatt taaatgtctt ctcctgaacg cgctcactgt gaaacattcg
     4081 cttgtcctac cattaaaaag agttatgttt ttaacggctc acgcccaaac ataactcttt
     4141 attaatggtc acaaggggga aacactcaat accgtaccta tttcttattg accattattc
     4201 tttttttggc aaaaaaatca aagcaattat cgttaaaatt atcgtcagaa tagcgatata
     4261 aactggcgca tttatgtttt gtacgtaaaa gtaacctgca ataatggggg caattaacgt
     4321 acctaaagat gtaaaggttg agttcataga acctgctaaa ccttgcttat cttttgcgat
     4381 ttttgataaa tatacagtaa ttgctggccg tataatatca aagcctataa aaataacaca
     4441 actaattaaa acaagtgcta tgacagaatt taagagtaac aaaatacata ataccattat
     4501 tgaataaata gagctaatga taataataga aaactcatgt aaatgccttg agattttagg
     4561 ataaaccaaa tattgtgtta gcgcccccaa caatgaacca ccgataatag caagggcaat
     4621 atgaaacgaa gtaaagtctg ccttatctac gagatataac ggatacaatt cttcaatcgc
     4681 agctaatccg aaagctaata aaagtatcac catcgctggt aataaataaa gtttcaaata
     4741 tttaaatgat aaagttgcta atttattttc ttttgatgcc aatgtttgaa catgtggtat
     4801 tttcaaattt aaattaatta ttaacataat gatacctata gccatagcta taaaatacgg
     4861 tagtctaaga tgaaatgtag caaacaatgc gcctatacca gggcctatga taaagcctaa
     4921 tgacgtgatt gctgaaaacg tgccgaaatt tttatcgcga ttagcttcat cagatatgtc
     4981 accaataata ccaataacgg aagtttggat taacgccgca cttataccac cgagtatcct
     5041 cgaagacaaa agtgtataga accccatacc aattccaaat aaaaattcag aaacgatata
     5101 aagtaacaag ccaatagata aaaagtgctt tttagcgtat ctatcagaaa aaaagccaac
     5161 tataggcgaa aaaatcattt gaaaaagaga aaatacagca ataagtatcc caaaagcatc
     5221 accatctata tttaaatctt taatgattaa tggcaagcta ggaacaacta aactaatagc
     5281 tgtaaatact aagaaaatat taatatatag tggaactatt aaactttttt taatcattgt
     5341 aactcctttg gggatacatg attaaagtta atatttcaat tataattcac gaaaaatgtt
     5401 tacataattg aggattctta aacatatatc ccagctaatt ttttattttg tacttttaat
     5461 cgatttatga tgactgacat aaatattcct cctttttaat gctcacatta tacaatatct
     5521 atttttaaat gaaaatagtc attttatctc ttcaaaataa ggtctaaatg aaccattaaa
     5581 ttcattctct tcctgttatt tcctttaata tctcttcact tgacttatag ttaaatatca
     5641 taagaggacg tgcattaatt tgttcaacat ctttcgcaaa atcatattgt tccattaaag
     5701 gaacatcaaa caaaacaggc tcattatatt atcctaaaat agagatataa cctcctccaa
     5761 tacctgattc atgtattgat tgaaattcct tatcagtttg gccaaagatc attttataac
     5821 catcagttga taaattacct aaagatacgc gtaatcccaa ttggtcacgt acattccctt
     5881 taatgacatc tgcatcagga cgttgcgctg ttaaaaacat aaatatacct gcatgatgac
     5941 ctttcataat aatatttaca agataatcat tcatttcctg taattctttt ttatctaatg
     6001 ttgatgtaac tgctgtaaac tcatcaaaaa taacgaagta tgcaggcata tcaaaacttc
     6061 taaaatcatg tccaattcga taatcttcgc tttccgtcat taatcggaaa cgattttcca
     6121 tttcttcatt cgctaatctc aattgtttaa atatctgacc tttcatatct gcaactttat
     6181 cagaactaat cacacgttta aatcaagtag gagaataatc attacttgat tattcgtccc
     6241 ctcacaccac cgagcgtatg gttctgtgcg cacatatttc cccttttttc gttccactac
     6301 ttatctctcc ataagttgcc aactagttcg ttgttttctg catgatgtaa atctataacc
     6361 tccattcttt actttgtatg aatattgttc cgtccttcgg tacattttcc ctactatgac
     6421 gtctgctgac ttctcattat tcgttgttac tacggtatgt gcaccgctaa tgagacctcc
     6481 ctgggtaagg
//
LOCUS       DAHHOJ010000123         6436 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.123, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000123 DAHHOJ010000000
VERSION     DAHHOJ010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6436
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.123"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            94..1725
                     /locus_tag="NBH32_002307"
     CDS             94..1725
                     /locus_tag="NBH32_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HCD1515879.1"
                     /translation="MKKLTTILFQYKIFPVLMFMVSTGLGILVITQNILIADFLAKII
                     RHQFQGLWIVLFILLGVLLLRATVQFLNQWLGDTLAFKVKHMLRQRVIYKNNGHPIGE
                     QMTILTENIDGLAPFYKSYLPQVFKSMMVPLIIIIAMFFIHFNTALIMLITAPFIPLF
                     YIIFGLKTRDESKDQMTYLNQFSQRFLNIAKGLVTLKLFNRTEQTEKHIYDDSTQFRT
                     LTMRILRSAFLSGLMLEFISMLGIGLVALEATLSLVVFHNIDFKTAAIAIILAPEFYN
                     AIKDLGQAFHTGKQSEGASDVVFEFLEQPNNNNEFLLKYEENQKPFIQLTDISFRYDD
                     SDRLVLNDLNLEIFKGDQIALVGPSGAGKSTLTHLIAGVYQPTIGTISTNKRDLNIGI
                     LSQQPYIFSASIKENITMFKDIENNTIEEVLDEVGLLDKVQSFTQGINTIIGEGGEML
                     SGGQMRRIELCRLLVMKPDLVIFDEPATGLDIQTEHMIQNVLFQHFKDTTMIVIAHRD
                     NTIRHLQRRLYIENGRLIADDRNISVNITENGDDL"
     gene            1722..3395
                     /locus_tag="NBH32_002308"
     CDS             1722..3395
                     /locus_tag="NBH32_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding/permease
                     protein"
                     /protein_id="HCD1515880.1"
                     /translation="MKTRLKFQVDKDLLLAIVVGVCGSLVALAMFFLSGYMVTQSALG
                     APLYALMILVVTVKLFGFLRAITRYVERLISHKATFTMLRDIRVQFFGKLVNVIPNVY
                     RKLSSSDLISRMISRVEALQNIYLRVYYPPVVIGLTALVTVIVLAFISISHALLIMVS
                     MLFTLLVVPWLSSKKARTLKKHAANEQARFLNHFYDYKAGMDELRRFNQINHYRDNLM
                     AKLNHFDKLQLKEQRFLTIYDFILNIIAMLSIFGSLVLGLIQINAGQLNIIYMTSIVL
                     MVLTLFEQAVPMTNVAYYKADTDQALHDINEVISVPSTNGKNRLNDKYDATNIYEVKD
                     ASFKYWNQQTYVLSDINFNVNRGEKIAIVGPSGSGKSTLLQIMAGLYQLDSGSIRFEN
                     MDMFEIDDKDKFESLNVLLQSQQLFDGTIRQNLFTDEKDEAVQAIFKQLDLEHLALER
                     QIDLDGHTLSGGEIQRLAIARMLLKDTVSTWILDEPTTALDKQNSLKVMDLIEAHAET
                     LIVATHDLTLLSRFETIIVMINGKIVEKGNYQQLLANQGALWNMIQYNA"
     gene            complement(3522..3965)
                     /gene="mgrA"
                     /locus_tag="NBH32_002309"
     CDS             complement(3522..3965)
                     /gene="mgrA"
                     /locus_tag="NBH32_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MgrA"
                     /protein_id="HCD1515881.1"
                     /translation="MSDQHNLKEQLCFSLYNAQRQVNRYYSNKVFKKYNLTYPQFLVL
                     TILWDESPVNVKKVVTELALDTGTVSPLLKRMEQVDLIKRERSEVDQREVFIHLTDKS
                     ETIRPELSNASDKVASASSLSQDEVKELNRLLGKVIHAFDETKEK"
     gene            4192..5118
                     /locus_tag="NBH32_002310"
     CDS             4192..5118
                     /locus_tag="NBH32_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1515882.1"
                     /translation="MKNNKDEKIRISIINGFLGSGKTTLLTHYISELLKNDEKIKIIM
                     NEFGTFDIDSNSISNEIEVHSLINGCVCCDLKQELVYELKAIALKGDVNHVIIEATGI
                     AHSLELLVACQDPQIVNFFEKPIIYGVLDATRFLERHQYTENTVSLMEDQLKLSDMII
                     INKIDLVTDDSLEKIDKQLSIICASIPTYKTTYGQVSLEELDLPGKDREISSHHHHHH
                     GIKSMTYTFTGPIDRQLFYQFIMKLPESVLRLKGYVSFRDQPNAIYEFQYAYGLPDYG
                     IIGMHLPLTIVIIGETLDTNHIRNQLDMLQFT"
     gene            5221..6129
                     /locus_tag="NBH32_002311"
     CDS             5221..6129
                     /locus_tag="NBH32_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCD1515883.1"
                     /translation="MEQIMINHYVHFSRLVQGFWRANEWKMTAKELNYFINELVERGI
                     TTMDHADIYGDYQCESLFGNALDLSPELRNKIQIVTKCGIILPSKQFDFTNGHRYDLS
                     SKHIVKSVEQSLINLNVDYLDSLLIHRPSPLMDPEQVADALTKLVKQGKLKSFGVSNF
                     NHSQYQLLNQYIMKERLHISINQLELSPYHVDSLQDGTMDSMYQNHVQIMAWSPFAGG
                     KIFDKEDIKAQRIMKVVQSIADKYGVSDTAVMIAWLVKIPHRIMPILGTSQLKRIDQA
                     IEGLQLNLDDQSWFDIYTAIIGQDIP"
     gene            6164..>6436
                     /locus_tag="NBH32_002312"
     CDS             6164..>6436
                     /locus_tag="NBH32_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515884.1"
                     /translation="MTNEDKRFEQLRFERKFIVIPYLIYAVIVLLLNIFYSDLKITMT
                     LFGLFFAYNVVILFIAFIKHYKRTLLLSLILTVLSGAAFFGIIYVYG"
BASE COUNT         2181 a          889 c         1137 g         2229 t
ORIGIN      
        1 attttctttt acatgtaaaa ctacaatatt ttattgagct atttaatttg attttaagga
       61 aaacctttta taataggttt aggtgatata attgtgaaaa aattaacaac aatactgttt
      121 caatataaaa tttttccggt actcatgttc atggtcagta ctggtctcgg catactcgtt
      181 ataacgcaaa atattttaat agcagatttt ttagctaaaa ttataagaca tcaatttcaa
      241 ggtttatgga ttgtattatt tattttatta ggtgttttac ttttaagagc aactgtgcaa
      301 tttctaaatc aatggttagg tgatacatta gcatttaaag ttaagcatat gcttagacag
      361 cgggttattt ataaaaataa tggtcatcca atcggtgaac aaatgactat actcacagaa
      421 aacattgatg gtctagcacc tttttataag agttatttgc ctcaagtgtt caaatcaatg
      481 atggttccgc tcatcataat cattgcaatg tttttcatcc atttcaatac cgcattaatt
      541 atgttaataa ctgcaccatt tattcctttg ttttatatta ttttcggttt gaaaacgcga
      601 gatgagtcaa aagatcaaat gacttatttg aatcaattta gtcaacggtt tttaaatatt
      661 gctaaaggtt tagtgacgtt aaagctattt aatcgtacag agcaaacaga gaagcatatt
      721 tacgacgata gtactcagtt tagaacttta acaatgcgca ttttacgcag tgctttttta
      781 tcgggattaa tgctcgaatt tataagtatg ttaggtattg gattggttgc attggaagca
      841 acgctaagct tagtagtatt tcataatatt gattttaaaa ctgcggcaat tgcgattatt
      901 ttagcgcctg aattttataa tgcaattaag gacttagggc aagcgttcca tactggaaaa
      961 caaagtgaag gtgccagtga cgttgtgttt gagtttttag aacaaccgaa caataataat
     1021 gaatttctat taaagtatga ggaaaatcaa aagccattta ttcagttaac agacatatca
     1081 tttcgatatg atgattctga tagattggta ttaaatgatt taaatttgga aatatttaaa
     1141 ggtgatcaaa ttgcacttgt aggtccaagc ggggcaggta aatccacttt gacacatctt
     1201 attgcaggtg tttatcagcc aacaataggt actataagta caaacaagcg tgatttaaat
     1261 ataggaatac ttagtcaaca gccatatatt ttcagtgctt ctataaaaga gaatattacg
     1321 atgtttaaag atatagaaaa taatactatt gaagaagtgc tagacgaagt aggtttatta
     1381 gacaaagtgc aatctttcac acaaggcatt aacacaataa taggtgaagg aggcgaaatg
     1441 ttatctggtg gacagatgag acgcatagaa ctttgccgtc ttttagttat gaagccagat
     1501 ctcgttatat ttgatgagcc tgcaactggt ttagatattc aaacagaaca catgattcag
     1561 aacgttctgt ttcaacattt taaagataca acgatgattg tcattgcaca tagagataat
     1621 acaattcgcc atttacaacg acgtttgtat atagaaaatg gaagactgat tgctgatgat
     1681 cgcaatattt cagtaaatat aacagaaaat ggtgatgact tatgaaaaca cgactaaaat
     1741 ttcaagtaga taaggattta ttgttagcta tagttgttgg tgtttgtgga agtttagttg
     1801 cgctcgccat gtttttctta agtggttata tggtgacaca aagtgcactt ggtgcgccac
     1861 tatacgctct gatgatttta gtcgtaacag taaaattgtt tgggttttta agagctatta
     1921 ctcgatacgt agagcgtctt atttctcata aagctacatt tacaatgcta cgtgatattc
     1981 gggtacagtt tttcggtaaa ttagtaaatg tcattcctaa tgtttaccgt aaactgagtt
     2041 ctagtgattt aatttcacgt atgattagtc gtgttgaggc attacaaaat atatatttac
     2101 gtgtttatta tccaccagtc gttatcggtt tgacagcgct agttacagta atagttttgg
     2161 cgttcatttc aatcagccat gcgctattga ttatggttag catgttgttt actttactcg
     2221 ttgttccttg gttaagctca aaaaaagcac gtactttaaa gaaacatgca gctaatgaac
     2281 aggcccgttt tttaaatcat ttttatgatt ataaagctgg tatggatgaa ctacgtcgat
     2341 ttaatcaaat taatcattat cgagataatt tgatggctaa attaaatcat tttgataaat
     2401 tacaacttaa agagcaacgc tttttaacga tttatgattt tatattaaat attattgcta
     2461 tgctttcgat ttttggtagt ttagttctag gattaattca aattaatgca ggccaactaa
     2521 atattattta tatgacgagt atagttttaa tggtcttaac tttatttgaa caagctgtac
     2581 caatgacaaa tgtcgcgtat tataaagcgg atactgacca agcattgcat gatattaatg
     2641 aagtgatatc tgtaccttct actaatggaa aaaaccgtct taatgataag tatgatgcaa
     2701 cgaacattta tgaagttaag gacgctagtt ttaagtattg gaaccagcaa acgtatgtgt
     2761 tgtcggatat taattttaat gttaatagag gcgaaaagat tgcgattgtg ggtccttctg
     2821 gttcaggaaa aagtacatta ctacaaatta tggctgggtt atatcaatta gatagtggct
     2881 ctattcgttt cgaaaatatg gatatgtttg aaatagatga caaagataag tttgaatcgt
     2941 taaatgtctt gctacaatct caacaattat ttgatggtac aatacgtcaa aatttattta
     3001 ccgatgaaaa agatgaagcg gtgcaagcaa tatttaagca attagattta gaacatttgg
     3061 cactagaacg tcaaattgac ttagatggtc atacattatc tggcggagaa attcagcgtt
     3121 tagcgattgc gaggatgtta ttaaaagata ctgtatcaac atggatttta gatgaaccaa
     3181 caactgcatt agataaacaa aatagtttaa aagttatgga tttaattgaa gcacatgcag
     3241 aaacattaat tgttgctaca cacgatttaa ctttattgtc acgtttcgag accatcattg
     3301 tgatgataaa tggtaaaata gttgaaaagg gaaactatca acaattactc gctaatcaag
     3361 gtgctttatg gaatatgatt caatataatg cataaaaaaa ctgcttgata ggctaagaaa
     3421 cctttcaagc agttttttaa ctaacaagct tacgcgtgct aattgttttt ctattcttaa
     3481 taaattctaa acattacttt aattgtcatg acaaaagtta attatttttc ctttgtttca
     3541 tcaaatgcat gaatgacttt acctaataag cgattaagtt ctttaacttc atcttgagat
     3601 aaagaagaag ctgaagcgac tttgtcagat gcattactta attctggtct aatagtttca
     3661 cttttgtcag tcaagtgaat aaatacttca cgttgatcga cttcggaacg ttcacgctta
     3721 attaagtcta cttgttccat tcgttttaat aatggtgata ctgtaccagt atcgagtgct
     3781 aattcagtta cgactttctt gacgtttaca ggagattcat cccataaaat tgttaagaca
     3841 agaaattgtg ggtatgttag attgtacttc ttaaaaactt tgttagagta gtagcgatta
     3901 acttgtcttt gagcattgta caaactaaag catagctgtt cttttaaatt atgttgatca
     3961 gacattaaag ttctcctcca gacatactat ccgttttttt ctcttttcgg attggtaatc
     4021 attaaaaagt tgattgttta ttaattcaca actttctttg attcaatgcc atgctaaaat
     4081 taaagtatgt ttaaagttta gaagatattt ttgattaaat caagcaaaaa gataatttaa
     4141 tatatatgtt atcattttta aaaataactg taatagaaaa gagaatataa aatgaaaaat
     4201 aataaagatg aaaaaataag aatatccata attaacggat ttttgggtag tggtaaaacc
     4261 acgttactga cacattatat tagtgaatta ttaaaaaatg atgagaaaat taaaatcatc
     4321 atgaatgaat tcggtacttt tgatattgat agcaatagta tttcaaatga aattgaagtc
     4381 cattcattga ttaatggttg tgtttgttgc gatcttaaac aagaacttgt ctatgaacta
     4441 aaagccattg ctttaaaagg ggacgttaat catgtcatca tagaagcgac aggcattgcg
     4501 cattctttgg aattactagt tgcatgtcaa gatccgcaaa tcgttaattt ctttgaaaaa
     4561 ccaattattt atggtgtatt agatgcgact cgatttttgg aacgtcatca atataccgaa
     4621 aatacagttt cgctgatgga agatcagttg aaactaagtg acatgattat tattaataaa
     4681 attgatcttg taactgatga cagtcttgaa aaaattgata agcaattaag tataatttgt
     4741 gcaagtattc caacttataa aacaacctat ggacaagttt cgttggaaga attggactta
     4801 cctggtaaag acagagagat atcgtctcat catcaccatc atcatgggat taaaagtatg
     4861 acttacacat ttacaggtcc gattgatcgt caattgtttt atcaatttat aatgaaatta
     4921 ccggaatctg ttctacgttt gaaaggttat gtatcattta gagatcaacc aaatgcaatt
     4981 tatgaatttc aatatgcata tggtttacca gactatggaa taattggcat gcacttacca
     5041 ttaacgattg ttattattgg tgaaacttta gatacaaatc acatacgtaa tcaattggat
     5101 atgctacaat ttacgtaaat cagaaaatgt gttgtttcaa tttaacggaa atagcacatt
     5161 aatttcttaa tacttttatt gtgaatattt tgtgacattg agggatggag tggatgagat
     5221 atggaacaaa taatgattaa tcactatgtt catttttcta ggcttgtaca aggtttttgg
     5281 cgtgcaaatg aatggaagat gactgcgaaa gagttaaatt attttataaa tgaattagtt
     5341 gaacgtggga ttaccacgat ggatcatgct gatatttatg gggattatca atgtgaatca
     5401 ctgtttggta atgctttgga tttatcaccc gaattaagaa ataaaattca aattgttacg
     5461 aaatgtggta tcattttgcc ttctaagcaa tttgatttta caaatggaca tcgttatgat
     5521 ttgagtagta agcacatcgt gaaatctgtt gaacagtcat taatcaattt gaatgtagat
     5581 tatttagata gtctactcat tcatcgtcct tcaccattga tggatccaga acaagttgct
     5641 gatgcattaa ctaaacttgt taaacaaggt aagttgaagt cattcggggt gtcgaatttt
     5701 aatcattcac aataccaatt gttaaatcaa tatattatga aagaaagact acatattagc
     5761 atcaatcaat tagaattatc gccatatcac gttgatagtt tacaagatgg aacaatggat
     5821 tcaatgtatc aaaaccatgt tcaaattatg gcttggagtc cttttgcagg cggtaaaatt
     5881 ttcgacaagg aagatattaa agcgcaacgt attatgaaag ttgttcaatc aatagctgac
     5941 aaatatggtg tgagtgacac agctgtgatg atagcatggt tagtaaaaat accgcatcgt
     6001 atcatgccga tacttggaac aagtcagtta aagcgtattg atcaagcaat cgaagggcta
     6061 caacttaatt tagatgatca gtcgtggttt gacatttaca ccgctattat cggacaagat
     6121 attccgtaaa cttatttact tttaaatcat aaaggagcat atcatgacaa acgaagataa
     6181 acgtttcgaa caattaagat ttgaacgcaa atttatagtt attccgtatt taatttatgc
     6241 agtcattgta ttactattaa atattttcta ttctgatttg aaaataacaa tgacattatt
     6301 cggacttttc tttgcgtata atgtagtcat tttgttcata gcatttatta aacattataa
     6361 acgcacattg ttactaagtc ttatattaac agtgcttagt ggcgcggcat tctttggaat
     6421 tatttatgtt tatggc
//
LOCUS       DAHHOJ010000124         6415 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.124, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000124 DAHHOJ010000000
VERSION     DAHHOJ010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6415
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.124"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            240..641
                     /locus_tag="NBH32_002313"
     CDS             240..641
                     /locus_tag="NBH32_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1515885.1"
                     /translation="MKKVNNDTVFGILQLETLLGDINSIFSEIESEYKMSREEILILL
                     TLWQKGSMTLKEMDRFVEVKPYKRTRTYNNLVELEWIYKERPVDDERTVIIHFNEKLQ
                     QEKVELLNFISDAIASRATAMQNSLNAIIAV"
     gene            complement(761..1324)
                     /locus_tag="NBH32_002314"
     CDS             complement(761..1324)
                     /locus_tag="NBH32_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCD1515886.1"
                     /translation="MKLERILPFSKTLIKQHITPESIVVDATCGNGNDTLFLAEQVPE
                     GHVYGFDIQDLALENTRDKVKDFNHVSLIKDGHENIEHHINDTHKGHIDAAIFNLGYL
                     PKGDKSIVTKPDTTIQAINSLLSLMSIEGIIVLVIYHGHSEGQIEKHALLDYLSTLDQ
                     KHAQVLQYQFLNQRNHAPFICAIEKIS"
     gene            complement(1321..2274)
                     /locus_tag="NBH32_002315"
     CDS             complement(1321..2274)
                     /locus_tag="NBH32_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500380.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCD1515887.1"
                     /translation="MGNHFQYAFENKRYHTWNYHLKNKFGQKIFKVALDGGFDCPNRD
                     GTVAHGGCTFCSAAGSGDFAGNRADSIAVQFKEIKEKMHEKWHEGKYIAYFQAFTNTH
                     APVEVLKEKFEPVLKEPGVVGLSIGTRPDCLPDDVVEYLADLNQRTYLWVELGLQTIH
                     QSTSDLINRAHDMKTYYDGVAKLRKHNINVCTHIINGLPGEDYDMMMATAKEVAQMDV
                     QGIKIHLLHLLKGTPMVKQYDKGLLTFMTQEEYTNLVVDQLEVIPPEMIVHRITGDGP
                     IDIMVGPMWSVNKWEVLNGIDAELARRNSYQGLRYKSKVKQ"
     gene            2385..3566
                     /locus_tag="NBH32_002316"
     CDS             2385..3566
                     /locus_tag="NBH32_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515888.1"
                     /translation="MNIPKSVWWLVIGMALNITGSSFLWPLNTIYMKQELGKSLTVAG
                     LVLMINSFGMVIGNLLGGSLFDKLGGYKTILIGTFTCLCSTTLLNFFHGWPWYAVWLV
                     MLGFGGGMIIPAIYAMAGAVWPNGGRQTFNAIYLAQNIGVAVGAAMGGFVAEFSFNYI
                     FLANLIMYVVFALVAVTQFNIEINAKVKYPTHLDITGKKNKARFISLVLICAMFAICW
                     VAYIQWESTIASFTQSINISMAQYSVLWTINGIMILVAQPLIKPVLYLLKGNLKKQMF
                     VGIIIFMLSFFVTSFAENFTIFVVGMIILTFGEMFVWPAVPTIANQLAPDGKQGQYQG
                     FVNSAATVGKAFGPFLGGVLVDAFNMRMMFIGMMLLLVFALILLMVFKENNTQPKKID
                     A"
     gene            3854..6271
                     /locus_tag="NBH32_002317"
     CDS             3854..6271
                     /locus_tag="NBH32_002317"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCD1515889.1"
                     /translation="MLNYNHNQIEKKWQDYWDENKTFKTNDNLGQKKFYALDMFPYPS
                     GAGLHVGHPEGYTATDIISRYKRMQGYNVLHPMGWDAFGLPAEQYALDTGNDPREFTK
                     KNIQTFKRQIKELGFSYDWDREVNTTDPEYYKWTQWIFIQLYNKGLAYVDEVAVNWCP
                     ALGTVLSNEEVIDGVSERGGHPVYRKPMKQWVLKITEYADQLLADLDDLDWPESLKDM
                     QRNWIGRSEGAKVSFDVDNTEGKVEVFTTRPDTIYGASFLVLSPEHALVNSITTDEYK
                     EKVKAYQTEASKKSDLERTDLAKDKSGVFTGAYAINPLSGEKVQIWIADYVLSTYGTG
                     AIMAVPAHDDRDYEFAKKFDLPIIEVIEGGNVEEAAYTGEGKHINSGELDGLENEAAI
                     TKAIQLLEQKGAGEKKVNYKLRDWLFSRQRYWGEPIPVIHWEDGTMTTVPEEELPLLL
                     PETDEIKPSGTGESPLANIDSFVNVVDEKTGMKGRRETNTMPQWAGSCWYYLRYIDPK
                     NENMLADPEKLKHWLPVDLYIGGVEHAVLHLLYARFWHKVLYDLGIVPTKEPFQKLFN
                     QGMILGEGNEKMSKSKGNVINPDDIVQSHGADTLRLYEMFMGPLDAAIAWSEKGLDGS
                     RRFLDRVWRLMVNEDGTLSSKIVTTNNKSLDKVYNQTVKKVTEDFETLGFNTAISQLM
                     VFINECYKVDEVYKPYIEGFVKMLAPIAPHIGEELWSKLGHEESITYQPWPTFDEALL
                     VDDEVEIVVQVNGKLRAKIKIAKDTSKEEMQEIALSNDNVKASIEGKDIMKVIAVPQK
                     LVNIVAK"
     gene            6293..>6415
                     /locus_tag="NBH32_002318"
     CDS             6293..>6415
                     /locus_tag="NBH32_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCD1515890.1"
                     /translation="MKSITTDELKNKLLESKPVQIVDVRTDEETAMGYIPNAKLI"
BASE COUNT         2142 a          922 c         1228 g         2123 t
ORIGIN      
        1 tgtaatagag tgatttgttt tatgtactat tatcttattt ctaaatatta actctattga
       61 ttattggttt ttatacttat ttaattttat tcaactttga caattgaata gaaagcaagt
      121 ttatttacac ttgtagtttt atgcataagt tagcacatac aagttttggg attgttggga
      181 tgtttgttaa tacttgtatg gtagctaaat atgtgattat taattgggag atgtttagca
      241 tgaaaaaagt aaataacgac actgtatttg ggattttgca attagaaaca cttttgggtg
      301 acattaactc aattttcagc gagattgaaa gcgaatacaa aatgtctaga gaagaaattt
      361 taattttact aactttatgg caaaaaggtt ctatgacgct taaagaaatg gacagatttg
      421 ttgaagttaa accgtataag cgtacgagaa cgtataataa tttagttgaa ttagaatgga
      481 tttacaaaga gcgtcctgtt gacgatgaaa gaacagttat tattcatttc aatgaaaagt
      541 tacaacaaga gaaagtagag ttgttgaatt tcatcagtga tgcgattgca agtagagcaa
      601 cagcaatgca aaatagttta aacgcaatta ttgctgtgta agtttaatag cataaaaaga
      661 ggttttcatt aagttgaaaa cctctttttg ttgttggtat taatttttca aatgttgact
      721 actcaatcct aaattataaa tagtatagcg cagcaaatgc ttaagaaatt ttttctatgg
      781 cacaaatgaa tggagcatga ttacgttggt ttaaaaattg atattgcaaa acttgcgcat
      841 gcttttgatc caaagtactc aagtaatcaa gcaatgcatg cttctcaatt tgtccttcgc
      901 tatgaccatg atatataaca agtacaataa taccttcaat tgacattaat gatagcaatg
      961 aattaatagc ttggattgtc gtgtcaggct ttgtcacgat agatttatca cctttaggca
     1021 aataacctag gttaaagatg gctgcatcaa tatgaccttt atgtgtatca tttatatgat
     1081 gttcaatatt ttcatgtcca tcttttatta aagaaacatg attgaaatcc ttaactttat
     1141 cacgtgtatt ttccaaagct aaatcttgaa tgtcgaaacc ataaacatgt ccttctggta
     1201 cttgttcggc taaaaataaa gtgtcattgc cgttaccgca agttgcgtct acaacaatac
     1261 tttctggtgt tatatgttgt ttaataagtg tttttgaaaa agggagtata cgttctaatt
     1321 tcattgcttc accttagact tgtaacgcaa gccttgataa gaatttctac gtgctaattc
     1381 agcatcgatg ccatttaata cttcccattt attaacactc cacattggac cgaccatgat
     1441 atctattgga ccatcaccgg taattcggtg aacgatcatt tcagggggaa tcacttctaa
     1501 ttgatccaca actaggtttg tgtattcttc ttgagtcata aaagttaata aacctttatc
     1561 gtattgtttt accatcggtg taccttttaa caaatgaagt aaatgaattt taataccttg
     1621 tacatccatt tgtgcaactt ctttggcagt agccatcatc atgtcatagt cttcgccagg
     1681 taagccatta atgatgtgtg tacatacatt gatattatgc ttacgtaatt ttgccacacc
     1741 atcataataa gttttcatat catgggcacg attgattaaa tcagatgttg attgatggat
     1801 tgtttgtagt cctaattcaa cccataagta tgttcgttga ttcaaatctg ctaaatattc
     1861 gacaacatcg tctggtagac agtcaggacg cgtaccaata gataatccca caacacccgg
     1921 ttctttaagt acaggttcga atttttcttt taatacttca actggtgcat gtgtatttgt
     1981 aaatgcctga aaataagcaa tatattttcc ttcgtgccat ttctcatgca tcttttcctt
     2041 aatttcttta aattgtactg cgattgaatc tgcacgatta cctgcaaagt ctccgctacc
     2101 tgcagcagaa caaaatgtac atccaccatg tgctacagtg ccatcgcggt taggacagtc
     2161 aaacccgcca tccaatgcaa ctttaaatat tttttgtcca aatttatttt ttaaatggta
     2221 attccacgtg tgataacgtt tgttttcaaa agcgtattgg aaatgattgc ccatatgtca
     2281 ttttcctttc tataaaaaaa agagttctaa gtacagattt taacatattt taatgttata
     2341 gtgtttatta tagtttgaca aaaaagagag aggaactatg aaatatgaat atacctaaat
     2401 cagtctggtg gctagtaatt ggcatggcgt taaatattac tggttccagt tttttgtggc
     2461 ctttaaatac aatttatatg aaacaagaac ttggaaaaag tttaactgtt gctggtttag
     2521 tgctaatgat aaattcattt ggcatggtta ttggaaactt attaggtggt tcactatttg
     2581 ataaattagg tggatacaag acgattttaa ttggaacttt cacttgtctt tgtagtacaa
     2641 cgctacttaa tttctttcac gggtggcctt ggtatgctgt atggcttgta atgttagggt
     2701 ttggtggcgg aatgattatt cctgcgatat acgctatggc tggagcagtg tggccaaatg
     2761 gcggaagaca aacgtttaat gcgatatact tagcgcaaaa tattggtgtg gctgtcggtg
     2821 ctgcaatggg cggctttgtc gcagaattta gctttaacta tatcttttta gccaatctta
     2881 ttatgtatgt tgtgtttgcg cttgtcgcgg taacgcaatt taatattgaa attaatgcga
     2941 aagttaaata tccaactcat ttagatatta ctggtaaaaa gaataaagca agatttattt
     3001 cattagtact aatttgtgca atgtttgcaa tttgttgggt tgcgtatatt caatgggagt
     3061 ctacaatcgc atcatttaca caatctatta atatttcaat ggcacaatat agtgttttat
     3121 ggacaattaa cggaataatg attttagtag cacaaccatt aattaaaccg gttctctatc
     3181 tgttaaaagg aaacttaaag aagcaaatgt ttgtcggcat catcattttt atgttgtcgt
     3241 tctttgtcac gagttttgcc gaaaacttta caatatttgt tgtcggtatg attattttaa
     3301 cttttggaga aatgtttgta tggccagcag ttccaactat agccaatcag ttagcgccag
     3361 atggtaagca aggacagtac caaggttttg tgaattcagc tgctacagta ggaaaagcat
     3421 ttggtccatt tcttggtggt gtattagttg atgcgtttaa tatgcgcatg atgtttatcg
     3481 gtatgatgct actacttgta tttgcattaa tattattaat ggttttcaag gagaataata
     3541 cgcaacctaa aaaaatagat gcataatgag taaatagaat taacgttata gacttgaaat
     3601 aaatgtcgtt ataacataat attaatttgt ataatttaat ttcgtttgga gcttttctac
     3661 agaaagctag tgatgctgag agctagtgtt aaggactaaa tgtaaatcgt attaatttta
     3721 aattgaatga atgacatctc ttactattaa aatgagtgca caatttttgt gaaatagggt
     3781 ggtaacgcgg caaatgtcgt ccctatgtaa atagaatagt tagaggtgtc ttttttattg
     3841 aataggagga aatgtgttga attacaacca caatcaaatt gaaaagaaat ggcaagacta
     3901 ttgggacgaa aataaaacat ttaaaacaaa tgataactta ggtcaaaaga aattttatgc
     3961 tttagacatg tttccatatc catcaggtgc tggtttacat gttggacatc ctgagggcta
     4021 tacagcaaca gatatcattt caagatataa aagaatgcaa ggatataatg tattacatcc
     4081 gatggggtgg gatgcattcg gattaccagc agagcaatat gctttagaca ctggcaacga
     4141 cccacgtgaa tttacaaaga aaaatatcca aacttttaaa cgacaaatta aagaattagg
     4201 gttcagttat gattgggatc gtgaagttaa tacaacagat ccagaatact ataaatggac
     4261 acagtggatt ttcatacagt tatataacaa aggtttagca tacgttgatg aagttgcagt
     4321 taactggtgt ccagcattag gcactgtttt atctaacgaa gaagtgattg atggtgtctc
     4381 tgaacgtggt ggacatccag tttatcgtaa gccgatgaaa caatgggtac ttaaaatcac
     4441 agaatatgca gatcaattat tagcagattt agatgattta gattggcctg agtctttaaa
     4501 agatatgcag cgcaattgga ttggacgttc tgaaggggcc aaagtttcat ttgatgtaga
     4561 taatacggaa ggaaaagtag aagtatttac gactagacca gatacaatct atggtgcatc
     4621 attcttagtc ttaagtcctg aacatgcatt agttaattca attactactg atgaatataa
     4681 agaaaaagta aaagcttatc aaacagaagc ttctaaaaag tcagatttag aacgtacaga
     4741 tttagcaaaa gataaatcag gtgtgtttac tggtgcatat gcaattaatc ctttatctgg
     4801 tgaaaaagta caaatttgga ttgctgatta tgtattatca acatatggta ctggagcaat
     4861 tatggcagta ccagcgcatg atgacagaga ttatgaattt gctaaaaagt ttgatttgcc
     4921 aatcattgaa gtcatcgaag gtggaaatgt tgaagaagca gcatacactg gtgaaggtaa
     4981 acatattaat tctggtgaac ttgatggttt agaaaatgaa gcggcaatta ctaaagctat
     5041 tcaattatta gagcaaaaag gtgctggcga aaagaaagtt aattacaaat taagagattg
     5101 gttattcagt cgtcagcgtt attggggcga accaattcct gtcattcatt gggaagatgg
     5161 aacaatgaca actgttcctg aagaagagct accattgttg ttacctgaaa cagatgaaat
     5221 caagccatca gggactggtg agtctccact agctaatatt gattcatttg taaatgttgt
     5281 agatgaaaaa acaggtatga aaggacgtcg tgaaacaaat acaatgccac aatgggcagg
     5341 tagttgttgg tattatttac gttacatcga tcctaaaaat gaaaatatgt tagcagatcc
     5401 tgaaaaatta aaacattggt tacctgttga tttatatatc ggtggagtag aacatgcggt
     5461 tcttcactta ttatatgcaa gattttggca taaagtcctt tatgatttgg gtatcgtacc
     5521 tactaaagaa cctttccaaa aattatttaa ccaaggtatg attttaggag aaggtaatga
     5581 gaagatgagt aaatctaaag gaaatgtaat caatcctgat gatatagtac agtctcatgg
     5641 tgcagatact ttacgtcttt acgaaatgtt tatgggacct ttagatgctg caattgcatg
     5701 gagtgaaaaa ggattagatg ggtctcgtcg attcttagat cgcgtatggc gtttaatggt
     5761 aaatgaagat ggaacattga gttcaaaaat tgtaactaca aataataaat ctttagataa
     5821 agtttataac caaactgtta aaaaggtaac agaagacttt gaaacattag gatttaatac
     5881 tgctattagt caattaatgg tatttattaa tgagtgttat aaagttgatg aagtttataa
     5941 accttacatt gaaggcttcg ttaaaatgtt agcacctatt gcaccacata tcggtgaaga
     6001 attatggtca aaattaggac atgaagagtc tattacgtac caaccttggc caacttttga
     6061 cgaagcacta cttgtagatg atgaagtaga aatcgttgtt caagtaaatg gtaaattgag
     6121 agctaaaatt aaaattgcta aagatacatc aaaagaagaa atgcaagaaa ttgccttatc
     6181 taatgacaat gttaaagcga gtattgaagg taaagacatc atgaaagtca tcgctgttcc
     6241 tcaaaaatta gtcaatattg tagctaaata atgttttaag gaggactttg aaatgaagtc
     6301 aattactaca gatgaattaa aaaacaaact tttagaatct aaaccagttc aaattgttga
     6361 tgttcgtact gacgaagaaa cagcaatggg atatattcct aatgcaaagt tgatt
//
LOCUS       DAHHOJ010000125         6351 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.125, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000125 DAHHOJ010000000
VERSION     DAHHOJ010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6351
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.125"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            219..1340
                     /locus_tag="NBH32_002319"
     CDS             219..1340
                     /locus_tag="NBH32_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001662816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HCD1515891.1"
                     /translation="MAELQRGLEGVIAAETKISSIIESQLTYAGYDIDDLAENAQFEE
                     VIFLLWNYRLPNEEELAHLKGKLNQYMTLNPRVYTHFEEYVTDHVHPMTALRTSLSYI
                     AHFDPDAENESDENRYERAMRIQAKVASLVTAFARVRQDKEPLKPNPDLSYAANFLYM
                     LRGELPTDIEVEAFNKALILHADHELNASAFTARCAVSSLSDMYSGIVAAVGSLKGPL
                     HGGANEQVMTMLSEIGSIENVDAYLDEKFANKDKVMGFGHRVYKDGDPRAKYLREMSR
                     QITKDAGREELFEMSVKMEKRMAEEKGLIPNVDFYSASVYHCMEIPHDLFTPIFAVSR
                     SAGWIAHILEQYKDNRIMRPRAKYIGETNRKYIPLEERK"
     gene            1389..2657
                     /gene="icd"
                     /locus_tag="NBH32_002320"
     CDS             1389..2657
                     /gene="icd"
                     /locus_tag="NBH32_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCD1515892.1"
                     /translation="MTAEKITQGTEGLNVPNEPIIPFIIGDGIGPDIWKAASRVIDAA
                     VEKAYNGEKRIEWKEVLAGQKAFDTTGEWLPQETLDTIKEYLIAVKGPLTTPIGGGIR
                     SLNVALRQELDLFTCLRPVRWFKGVPSPVKRPQDVDMVIFRENTEDIYAGIEFKEGTT
                     EVKKVIDFLQNEMGATNIRFPETSGIGIKPVSKEGTERLVRAAIQYAIDNNRKSVTLV
                     HKGNIMKFTEGSFKQWGYDLALTEFGDQVFTWQQYDEIVEKEGRDAANSAQEKAEKEG
                     KIIIKDSIADIFLQQILTRPAEHDVVATMNLNGDYISDALAAQVGGIGIAPGANINYE
                     TGHAIFEATHGTAPKYAGLNKVNPSSVILSSVLMLEHLGWQEAADKITDSIEDTIASK
                     VVTYDFARLMDGAEEVSTSAFADELIKNLK"
     gene            3047..3751
                     /locus_tag="NBH32_002321"
     CDS             3047..3751
                     /locus_tag="NBH32_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1515893.1"
                     /translation="MSQKVLVVDDEHSIVTLLKYNLETAGYEVVVAFDGDEALEKVEN
                     EQPDLIILDVMLPKKDGIDVCKTVRTNKNLVPILMLTAKNDEFDRVLGLELGADDYMT
                     KPFSPREVVARVKAILRRSQFVKEVEEIDDDITIGSIRIRPDYFEVYKHNELLELTPK
                     EFELLLYLIERQGRVITREHMLNTVWNYEFAGDSRIVDVHISHLRDKLEDNPKKPQLI
                     KTVRGLGYKLERPKEQ"
     gene            3751..5412
                     /locus_tag="NBH32_002322"
     CDS             3751..5412
                     /locus_tag="NBH32_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HCD1515894.1"
                     /translation="MKFHHRLMLLISSILIISFVTLGIIIHASITYVLSEKHEQALAT
                     DARSYIYLVQDNEIDKVKEIVKQQNIDLLITKNDKKVFSSGKLKATQNSDSYIQKKDV
                     FIFNKKVNGYHVWIKGYNNDITEMHWTLWKYLILTCLVVLICLYFASRSFKRTLIRPI
                     QEVTYATQLLANGYYHIRVPESNVIETKALFVSTNDLARRLQKLNNEQKMQSNRLKTT
                     IENIPSAILMIDRNGKIVVANKAYYEQFNISHNIEQVGYHGYVNTEIEQLILESFKVE
                     KPIYEQLEVAINQVHAKYFDISCVPILTRSQKSLQGILVVMHDITNLKQLENLRREFV
                     ANVSHELKTPITSIKGFAETLIDGAKNDAESLDMFLNIILKESNRIESLVTDLLDLSH
                     IEQHTELDTDYMNLSDLTRRIIDNMMTQANQKNISIHTDIEKDVIVKAQESKIAQVIT
                     NLLTNAINYSYEDGDINVRVYRDDFRVIFEVQDFGIGIKLEDQQRIFERFYRVDKARS
                     RDSGGTGLGLSITKHIVEAHQGNIEVNSQVGKGSTFKVILKDYKE"
     gene            5911..>6351
                     /locus_tag="NBH32_002323"
     CDS             5911..>6351
                     /locus_tag="NBH32_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515895.1"
                     /translation="MKTIQLHKTTLILIAILLFYTFMGILLPLMHDDLQWFSNYNTDI
                     LKVGFASLNGRYIGNIFEIIAVHVSWLRWLSYGLISMGIIWMIMHITRCKAWTSYYLL
                     AFSLMLILPSAIYADTYGWFAGFYNYATSTLISLFIIYYCINAII"
BASE COUNT         2290 a          832 c         1211 g         2018 t
ORIGIN      
        1 aattgaaaat tttattctat gctaaaagtt ggtgatgaat ataatcttga aaaagcttta
       61 atagagctat ttgtggctat tctgtgacat ttacatagaa ttccaaaata attgttgcac
      121 atataatgcc agtttttata tttcacaaac gaataacgtt tagctataat attagttgaa
      181 agccatttca taaagaaaca gtaaagggga aatttatcat ggcagaatta caaagaggtt
      241 tagaaggggt tatcgcagcg gagactaaga taagttcaat tattgaaagt caattaacat
      301 atgccggcta tgatattgat gatctagctg aaaacgcgca atttgaagaa gtcattttcc
      361 tattatggaa ttacagattg ccaaatgaag aagagctagc tcatttaaag gggaaattaa
      421 atcaatacat gacattaaat cctcgtgtgt atacacattt tgaggagtat gttacagatc
      481 acgtgcatcc aatgacagca ttacgtacgt cattatcata tattgcacat ttcgatcctg
      541 atgcagaaaa tgaatcagat gaaaatcgtt atgaaagagc aatgcgtata caggctaaag
      601 tagcatcatt agttacagcg tttgctcgag taagacaaga taaagaacca cttaagccta
      661 atcctgactt aagttatgcg gcaaacttcc tatatatgtt acgtggggaa ttaccaacag
      721 atatagaagt agaagccttc aataaagcac ttattttaca cgctgatcat gagttgaacg
      781 catctgcatt tacagcacgt tgtgcggtat catcattgtc agatatgtac tcaggtattg
      841 tagcagctgt aggttctctg aaagggccat tacatggtgg tgcaaacgaa caagttatga
      901 cgatgttatc tgagattggg tcaattgaaa atgttgatgc ttacttagat gaaaaatttg
      961 cgaataaaga taaagtaatg ggcttcggtc atcgtgtata taaagatggt gatcctagag
     1021 cgaaatattt aagagaaatg agccgtcaaa ttacgaaaga cgctggtcgt gaagaattat
     1081 ttgaaatgtc agtgaaaatg gaaaaacgta tggcagaaga aaaaggatta attcctaatg
     1141 ttgattttta tagtgcgagt gtttatcact gtatggaaat acctcatgat ttattcacgc
     1201 caatctttgc tgtaagtcgt tctgcaggat ggattgctca tattttagaa caatataaag
     1261 ataatagaat tatgcgtcct agagcaaaat atattggcga aacgaatcgt aagtatattc
     1321 cgcttgaaga aagaaaataa tcgatacaaa ttaaaaatga agatgtaaaa tttggaggta
     1381 aaataactat gactgcagaa aaaattactc aaggaactga aggattaaac gtacctaatg
     1441 aaccaattat cccatttatt atcggtgatg gaattggacc ggatatttgg aaggcagcaa
     1501 gccgagtgat agatgctgct gttgagaaag cttataatgg cgaaaaacgc attgaatgga
     1561 aagaagtgct agctggccaa aaagcatttg atacaactgg cgaatggtta cctcaagaaa
     1621 cacttgatac aattaaagaa tatttaattg ctgttaaagg acctttaaca acaccaattg
     1681 gtggtggtat tagatcatta aatgtggctt tacgccaaga attagattta tttacttgtt
     1741 taagaccggt acgttggttt aaaggagtac catcacctgt taaacgtcca caagatgttg
     1801 atatggttat tttccgtgaa aatactgaag acatttatgc tggtattgaa tttaaagaag
     1861 gtacaacaga agttaaaaag gtaattgact tcttacaaaa cgaaatgggt gcgacaaaca
     1921 ttcgattccc agaaacttca ggtattggta ttaaaccagt ttctaaagaa ggtactgagc
     1981 gattagttag agcagctatt caatatgcta tcgataataa ccgtaaatca gttactttag
     2041 ttcataaagg taatattatg aaatttacag aaggctcatt taagcagtgg ggttacgatt
     2101 tagcattaac tgaatttggt gatcaagtat ttacttggca acaatatgac gaaattgttg
     2161 aaaaagaagg cagagatgct gctaattctg cacaagaaaa agctgaaaaa gaaggcaaga
     2221 ttatcattaa agattctatt gctgacattt tcttacaaca aattttaact cgtccagctg
     2281 agcatgatgt tgtagcaact atgaacttga atggtgacta tatttcagat gctttagctg
     2341 cacaagttgg tggtattggt attgcgccag gtgcaaacat taattatgaa acaggtcatg
     2401 ctatttttga agcaacacat ggtacagctc caaaatatgc aggtttaaat aaagtgaatc
     2461 catcttcagt aattttaagt tctgtattaa tgttagaaca tttaggatgg caagaagcgg
     2521 cagataagat tacagattca attgaagata caattgcttc aaaagttgtt acttatgact
     2581 ttgcccgttt aatggatggt gctgaagaag tttctacatc agcatttgca gatgaattga
     2641 ttaaaaattt aaaataaata ctaattattt aggtgagtct agctacttta attgctaggt
     2701 tcgccttttt aaatgaattg aagttggcag caggacagaa ataataaaga gtcactaatg
     2761 attgattata tagtgtttct tacacattag acaaagcgat tgtgtacata agtttaggaa
     2821 taaatgagta aaactcatgc ataggtagtg gcatagaaat gatattttca caaagtttat
     2881 ttcgttgtcc cagagtaagt catgtaaatt atagaaggaa atataagaag tattttattt
     2941 atgtgtaaag taatgtaaaa aatgtgtaaa gataatgtgt aggatgttat ttttataaat
     3001 agttcggtta taatgtaaat ataattataa gttagggagg cataccatgt cgcaaaaagt
     3061 gttggtagta gatgacgaac attcaatcgt aacattactt aaatataact tagaaacagc
     3121 tggttatgaa gttgttgtcg catttgatgg tgatgaggct ttagaaaagg tggaaaatga
     3181 acagccagat ttaattattt tagatgttat gctacctaaa aaagatggca ttgacgtatg
     3241 taagactgta agaacaaata aaaatttagt ccctatatta atgttaacgg cgaaaaatga
     3301 tgaatttgat cgggtattag gtttagaatt aggtgccgat gactatatga ctaagccttt
     3361 ttcacctaga gaagttgttg cccgtgtgaa agccatttta agacgttctc aatttgtaaa
     3421 agaagtagaa gagattgacg atgatattac catcggatcg atacgtatta gacccgatta
     3481 ttttgaagta tataagcata atgagctatt ggagctaaca ccgaaagaat ttgaactgtt
     3541 gctttatttg atagaaagac aaggcagagt cattacgaga gagcatatgt taaatacggt
     3601 atggaattat gaatttgcag gagactcaag aattgttgat gtacatatca gtcatttaag
     3661 agataaatta gaagataatc ctaagaagcc acaacttatt aaaactgtgc gtggcttagg
     3721 ctataaattg gaaagaccta aagaacaatg atgaagtttc accaccgctt aatgttattg
     3781 ataagcagta tattaattat cagttttgtt acattaggca ttattattca cgcttccatc
     3841 acatatgtgt tatcagaaaa acacgaacaa gctctagcaa cagatgcaag atcatatatt
     3901 tatttagtcc aagataatga gattgataaa gttaaagaaa ttgtaaaaca gcaaaatata
     3961 gatttattga taactaaaaa tgataaaaag gtgtttagta gtggcaaact gaaagccact
     4021 caaaatagtg atagctatat tcaaaaaaag gacgttttta tttttaataa gaaggttaac
     4081 ggttatcatg tatggattaa aggttacaat aacgatatta cagaaatgca ttggacgttg
     4141 tggaaatatc ttattttgac atgtttagtt gtactcattt gtctgtattt tgcaagccgt
     4201 agttttaagc gtacattaat ccgtcccatt caagaagtta cttatgcaac acaattgttg
     4261 gcaaatggtt attatcacat acgtgtacct gaaagtaatg ttatagaaac taaagcgctt
     4321 tttgtgtcta ccaatgatct agcacgacgc cttcaaaagc ttaataatga gcaaaaaatg
     4381 caatccaatc gtttaaaaac aacgattgaa aatattccaa gtgctatttt aatgatagat
     4441 cgaaatggaa aaatcgttgt tgcaaataaa gcgtattatg aacagttcaa catctcgcat
     4501 aatattgaac aagtaggcta tcacggttat gttaatactg aaattgagca attaatttta
     4561 gaaagtttta aagttgaaaa acctatttat gaacaattgg aagttgcaat aaaccaagtc
     4621 catgccaagt actttgatat ttcatgcgtt ccgattttaa ctaggtctca aaaaagtttg
     4681 caagggattt tagttgtgat gcatgacata acaaatctga aacaacttga aaatctaaga
     4741 cgtgaatttg tagcaaatgt ttcacatgaa ttaaaaacac ctattacttc aattaagggg
     4801 tttgctgaaa cgcttattga tggtgcaaaa aatgatgcgg aatcattaga catgttttta
     4861 aacataattt taaaagaatc aaatcgaata gaatctttag taacagatct gttagattta
     4921 tcgcatattg agcaacatac agagttagat acagactata tgaatttatc agatttaacg
     4981 cggcgtatta ttgataatat gatgactcaa gccaatcaaa aaaatatttc cattcatact
     5041 gatattgaaa aagatgtcat tgttaaagca caagaaagta aaattgctca agttatcacg
     5101 aatttgttaa cgaatgcaat taactattct tatgaagatg gagatattaa cgttcgtgtg
     5161 tatcgagatg actttcgtgt cattttcgaa gtacaagatt ttggtatagg tattaaatta
     5221 gaagaccaac aacgtatttt tgaaagattt tatagagtag ataaagcacg aagtagagat
     5281 tctggtggga caggtcttgg attatcaatt acaaagcata tagtagaggc ccaccaaggc
     5341 aatattgaag tgaatagtca agttggcaaa ggctcaacgt tcaaagttat tctaaaagat
     5401 tataaagaat aaaaattaaa gtggtaacag cgcgtgtatt tttcacgaag ttgttgctgc
     5461 ttttttattt cgttcaaatc tctattggta ttaaattaga gttaatgaag tggaataaat
     5521 tcggttgtca attttgtcat ttgcatatgt cgatttgtaa gttataagta atagattgtt
     5581 gtgaatcaag caattttata atatatttat tataataaag aaattttagt aacgcgctta
     5641 aaatagatgc ttaattaaaa agtaataagt tttatttgaa cggaaataaa tcttaattta
     5701 aacacttgaa tagacaagtg taatcattcg tcatagaaaa agacgagggg cgatttagat
     5761 aaattgttga aagtaatatt aaataccttg tttatctaat ttgattgagg aaacatgcat
     5821 aggtgaaaat tagaaataca ttaaaaatat tgaattaatt tttcgaccta caagttgagg
     5881 aaaatttaac tagatttgag gattagcaaa gtgaaaacaa ttcaattaca caagacaact
     5941 ttgatattaa ttgcgatatt attgttttat acttttatgg gtatcttact accactcatg
     6001 cacgatgatt tacaatggtt tagcaattat aatactgata ttttaaaagt aggatttgca
     6061 tcacttaatg gtcgctatat cggaaatatc tttgaaatta tagctgtaca tgtaagctgg
     6121 ttacgttggc tttcatatgg tctcattagt atgggcatca tttggatgat tatgcacatt
     6181 acacgttgta aagcgtggac aagctattat ttgttagcat tttctttaat gttaatttta
     6241 ccaagtgcta tttatgcaga tacatatggc tggtttgcag gattttataa ttatgcgaca
     6301 tcaacactaa tttcactctt tattatttat tattgtatta atgcaattat a
//
LOCUS       DAHHOJ010000126         6224 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.126, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000126 DAHHOJ010000000
VERSION     DAHHOJ010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6224
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.126"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(179..610)
                     /gene="bstA"
                     /locus_tag="NBH32_002324"
     CDS             complement(179..610)
                     /gene="bstA"
                     /locus_tag="NBH32_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacillithiol transferase BstA"
                     /protein_id="HCD1515896.1"
                     /translation="MGLGYLVNVYDAWKVEKVLDDYHKPFSNTIHWQFGHVLTIFESA
                     LAVAGKENIDLNIYRPLFGNGSSPDEWKDEVPSIERILEGLQTLPERARNLTEDDLAI
                     ELKQPIVGCNNLEELLVLNAIHIPLHAGKIEEMSRILKNLK"
     gene            complement(695..1333)
                     /gene="pcp"
                     /locus_tag="NBH32_002325"
     CDS             complement(695..1333)
                     /gene="pcp"
                     /locus_tag="NBH32_002325"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HCD1515897.1"
                     /translation="MHILVTGFAPFDNQDINPSWEAVTQLENIIGTHTIDKLKLPTSF
                     KKVDTIINKTLASNHYDVVLAIGQAGGRNAITPERVAINIDDARISDNDDFQPIDQAI
                     HLDGAPAYFSNLPVKAMTQSIINQGLPGALSNSAGTFVCNHVLYHLGYLQDKHYPHLR
                     FGFIHVPYVPEQVIGKPDTPSMPLEKIVAGLTAAIEAISNDEDLHLALGTTE"
     gene            complement(1485..2441)
                     /locus_tag="NBH32_002326"
     CDS             complement(1485..2441)
                     /locus_tag="NBH32_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HCD1515898.1"
                     /translation="MNYQVLLYYKYTTIDDPEQFAQDHLAFCKAHHLKGRILVSTEGI
                     NGTLSGTKEETEQYMAHMHADERFKDMVFKIDEAEGHAFKKMHVRPRKEIVALDLEDD
                     VDPRHTTGQYLSPVEFRKALEDDDTVIIDARNDYEFDLGHFRGAIRPNITRFRDLPDW
                     IKENKALFADKKVVTYCTGGIRCEKFSGWLLKEGFEDVAQLHGGIATYGKDPETKGEY
                     WDGKMYVFDDRISVDINQVEKTIIGKDWFDGKPCERYINCANPECNKQILVSEENEAK
                     YLGACSYDCAKHERNRYVQANNISDNEWQQRLTNFDDLHQHA"
     gene            2685..3656
                     /locus_tag="NBH32_002327"
     CDS             2685..3656
                     /locus_tag="NBH32_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HCD1515899.1"
                     /translation="MSQQDLPTLFYSGKSNSAVPIISESELQKITAEPWLEISKKGLQ
                     LEGLNFDRQGQLFLLDVFEGNIFKVNPETKEIKRPFISHKANPAAIKIHKDGRLFVCY
                     LGDFKSTGGIFTATENGDNIQDIIEDLSTTYCIDDMVFDSKGGFYFTDFRGYSTNPLG
                     GVYYVAPDFRTVTPIIQNISVANGIALSTDEKVLWVTETTANRLHRIALEDDGVTIQP
                     FGATIPYYFTGHEGPDSCCIDSDDNLYVAMYGQGRVLVFNKRGYPIGQILIPGRDEGH
                     MLRSTHPQFIPGTNQLIICSNDIEMGGGSMLYTVNGFAKGHQSFQFQ"
     gene            complement(4134..4649)
                     /locus_tag="NBH32_002328"
     CDS             complement(4134..4649)
                     /locus_tag="NBH32_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyisoprenoid-binding protein"
                     /protein_id="HCD1515900.1"
                     /translation="MTNFTFDGAHSSLEFQIKHLMVSKVKGSFDQFDVAVEGDINDFS
                     TLKATTTIIPSSINTKNEARDNHLKSGDFFGTDEFDKITFVTKSITENKVVGDLTIKG
                     ITNEETFDVEFNGVSKNPMDGSQVTGIIVTGTINREKYGINFNQALETGGVMLGKDVK
                     FEASAEFSISE"
     gene            4837..4944
                     /locus_tag="NBH32_002329"
                     /pseudo
     CDS             4837..4944
                     /locus_tag="NBH32_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501466.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            5062..6186
                     /locus_tag="NBH32_002330"
     CDS             5062..6186
                     /locus_tag="NBH32_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCD1515901.1"
                     /translation="MHKKYLLLFFLLLSVALLYSCSKQNEKAQNKYVNPNEYAKHKSK
                     MDEHWETYHGQVYHVFYHPLITDPKVAFTGDTHQAKGNNDWMITVSEFKKSLNELYKN
                     NYIIVNPHDVYDLSSHHVKAKKIKLPKGKKPLIISIDDMNYYSYMRHHGYADRLVLDK
                     NKHVVSETTDSKGHATYSDDNDIVPILNKFVKEHPDFSLNGEKGVVALTGYEGVLGYR
                     TNELTSKDYTKNKKAVEEVVRAMKRDGWSFASHSYGHINFEKTSLEGIKRDTKRWKDE
                     VEPIVGKTDMFVFPHGAQDRYTQAYDYLVDEAEFKFIAGVGPNNFTDISATNVYQDRV
                     AIDGLNLFEFKYKLKPFFNPENVYSKQDRQFFKGNRDYEE"
BASE COUNT         2167 a          987 c         1008 g         2062 t
ORIGIN      
        1 aaaagattta actcataaac cattataatt atttttatag atatggctat tcctagttct
       61 atgtataata catgatatta ataggtcact tttaatctgt atgtaagcag actaaaagtg
      121 gccttttaaa aaataaaaaa atctgttaac gtgttgttaa cggttaataa gcacatattt
      181 attttaaatt ttttaatata cgagacatct cttcaatttt accagcatga agtgggatgt
      241 gaatggcatt taatactaat aactcttcta agttattaca accgacaatt ggctgtttca
      301 attcaattgc taaatcatct tcagttagat ttcgtgcacg ttcaggtaaa gtttggagac
      361 cttctaaaat cctttcaata ctcggtactt catccttcca ttcatctgga gacgaaccat
      421 ttccgaataa aggtctatag atatttaaat caatattctc tttaccagca acagctaagg
      481 ccgattcaaa aattgttaat acatgaccaa attgccaatg aatggtatta gaaaaaggct
      541 tatgataatc atcaagtacc ttttcaactt tccaagcatc atacacattt actaaatatc
      601 ctaaccccat aaataaaaca tacttccgta agtattaccc tattaacaaa gttgttagtc
      661 atttaaggaa tgtattttat tggtgtactt ataactattc cgttgtgccc agggctaggt
      721 gtaaatcctc atcattagaa atagcttcaa tggctgcagt caaacctgca actatctttt
      781 ctaatggcat agatggtgta tcgggtttac caatgacctg ctctggtacg tatggcacat
      841 gaataaatcc gaatcgtagg tgagggtaat gcttatcttg taaataacct aagtgataaa
      901 gtacgtgatt acatacgaac gtacctgcgc tatttgaaag tgctccagga agtccttgat
      961 taataatact ttgagtcatt gctttaactg gtaaatttga aaaataagct ggcgcaccgt
     1021 ctaaatgaat ggcttgatca ataggttgaa aatcatcatt atctgaaata cgtgcatcat
     1081 caatattaat ggcgacacgt tctggggtaa tggcatttct accaccagct tgtcctatag
     1141 ctagtacaac atcatagtga ttagatgcca acgttttatt tataatagta tctactttct
     1201 taaaagaggt tggtagtttt aatttatcga tggtatgtgt gccaataata ttctctagtt
     1261 gagtcacagc ttcccaagaa ggattaatat cttgattgtc aaaaggcgca aaccctgtta
     1321 ctaaaatgtg cattttaaga caccccgcag tattaaaatt taatttagta taacatgcgc
     1381 ataattaaaa acatatcagg ttatgcatgc gcataaaaaa cagccttgaa aaacctttat
     1441 aaatcggatt ttcaaagcgt gttttgaaag gtattttatt aaaactaagc atgttgatgt
     1501 aaatcatcaa agtttgttaa acgttgttgc cactcattat cactaatatt attcgcttga
     1561 acataacgat tacgctcatg tttagcacaa tcataagagc atgcacctaa atatttagct
     1621 tcgttttctt cggaaactaa tatttgttta ttacattctg ggttagcgca attaatataa
     1681 cgttcacatg gtttgccatc aaaccaatcc ttaccaataa ttgttttttc aacttggttg
     1741 atatcaacac tgatacggtc atcaaatacg tacattttac cgtcccaata ttcacctttt
     1801 gtttcaggat ctttaccata tgtagcaata ccgccatgaa gttgagctac atcttcgaaa
     1861 ccttctttta aaagccatcc agaaaatttt tcacatcgaa tgccaccagt acagtacgta
     1921 accacttttt tatctgcaaa taacgcttta ttctctttaa tccagtcagg caaatctcta
     1981 aagcgcgtga tatttggacg aattgcacct cggaaatgac ccaaatcaaa ttcataatca
     2041 ttacgtgcat caataatgac tgtgtcatca tcttcaagag cttttctaaa ttctacaggt
     2101 gataaatatt ggccagttgt gtgtcttgga tcgacgtcat cttctaaatc taaagcaacg
     2161 atttcttttc gaggacgtac atgcattttc ttaaaagcat gtccttcagc ttcatcaatt
     2221 ttaaacacca tatctttgaa tcgttcatcg gcatgcatat gtgccatata ttgttcggtt
     2281 tcttcttttg taccagataa tgtgccgtta ataccttctg tagaaacaag aattctacct
     2341 tttaaatggt gtgctttaca aaaggctaag tgatcctgag caaactgttc agggtcatca
     2401 atcgtcgtat atttataata taaaagaact tgatagttca taggttaatc tccttctaaa
     2461 aacatgttat aagcatgtta atttttaaaa taaagcgtca atacgcatat ttgtctacgt
     2521 ccttcatttt aacagagttg tattgtcatt caatatttct agcttgaaat cagccactag
     2581 accgataaaa tagataaacg aaaccgttat catttgattt aaatgtttcc ttgtttaaaa
     2641 taaaaatgat agttaaaacg aatgaaatga aaggaatgat catgatgtca caacaagatt
     2701 tacctacatt attttatagc gggaagtcta atagtgctgt tccaattata tctgaaagtg
     2761 aattacaaaa aattacagct gaaccatggc ttgaaatttc caaaaaagga ttgcaattag
     2821 aaggattgaa ctttgatcgg caaggacaac tatttttatt ggatgtattc gaaggtaata
     2881 ttttcaaagt caatcctgaa acgaaggaaa tcaaacgacc ttttataagt cacaaagcga
     2941 atcctgcagc aatcaaaata cataaagacg gccgattatt cgtttgttat ttaggagatt
     3001 ttaaatctac aggagggatt tttacagcta cagaaaatgg tgacaacata caagatatta
     3061 ttgaagatct ttcaacaaca tattgtattg atgacatggt atttgattct aaaggtggat
     3121 tttattttac agattttaga ggatactcta ccaatccact aggaggcgtt tattatgttg
     3181 cgccggactt tagaacagtg acgcctatca ttcaaaatat tagcgtagca aatggtattg
     3241 ctctaagtac agatgaaaaa gtactatggg taacagaaac tacagccaat cgattacatc
     3301 gcattgcact tgaagatgat ggtgtgacga tacaaccatt tggagctact ataccgtact
     3361 attttacagg tcatgaagga ccagactcat gttgtattga tagtgacgat aatttatacg
     3421 tagcaatgta tggtcaaggt cgagtgttag tttttaataa aaggggttat ccaataggac
     3481 aaatattgat accaggccga gatgaagggc atatgttacg ttctactcat ccgcaattta
     3541 tacctggaac aaatcaactc atcatttgtt ccaatgatat agaaatgggc ggaggatcta
     3601 tgctttatac agttaatggc tttgcaaaag gtcatcaaag ttttcagttt caataagacc
     3661 tacctgaatg aaaggcgtct taatataagt ttaaatctta gcaattttta atttagaagt
     3721 taaatataat ttgcttcaat ttgtaaaatg aagttttgta taacgtaaat aggcaatatt
     3781 taatataaga aagtataaag aagaattttc ttgattattt ctaatgaagc caatgccaat
     3841 ttaataagga gttgtttatt ttggctattt atattttttg tacaatacta gttttagtcg
     3901 cagttggagc acttttgaca aatagaataa gggataaaaa agataaaagg ctagatattt
     3961 tatctagtgt attactttta atttcatcaa taacattgtt actttatggt gttattatca
     4021 attaagttaa atatgatttt taatttagaa gttaaaaaat gccaactata cctagggatg
     4081 atatagttgg catttcgtat atatattaaa acaatgagga ttgtgtaatg agattattct
     4141 gagattgaaa attcagctga tgcttcgaat ttcacatctt tacctagcat tactccacca
     4201 gtttcaagtg cttggttgaa gttaatgcca tatttttctc tattgattgt accagtaaca
     4261 ataatacctg ttacttgaga accatccata ggattcttac ttactccgtt gaattcaacg
     4321 tcgaatgttt cttcgttagt gataccttta attgttaaat caccaacaac tttgttttct
     4381 gtaattgatt ttgtcacaaa agtgatttta tcaaattcat cagtaccaaa gaaatcacca
     4441 gattttaagt ggttatcacg tgcttcgttt ttagtgttaa ttgagcttgg aataattgtt
     4501 gtagtagctt tcaaagtact gaagtcatta atatctcctt caacagctac atcaaattga
     4561 tcaaatgaac ctttcacttt agaaaccatt aaatgtttaa tttggaattc taaactactg
     4621 tgtgcaccat caaaagtaaa gttagtcata atataaaacc tccatatttt tatctcttag
     4681 atatattttt tatacttgat atacatacaa taccacctaa aaaacaaaga tgcaagcatt
     4741 ttatttcgaa ttcgaatatt tttctgtgat gtatttaaag aatgacctat aaaacaccac
     4801 actattgtta taccctagtc aattaattca aacataaata agtgtaacca acgttcaata
     4861 aatttagcag aaaggcttaa attcaagcga gacaaagcta tggacgacat tatcaatcaa
     4921 ggagatattg tgttttataa ataaaatact atgacattat ctaaaaaata aaattaaaat
     4981 accaacagtt tcttaaaaag ttatgagcca cataataaaa atgttataat tgtgcataat
     5041 aaaacatagg gagaatgttt aatgcataaa aaatatttat tgctattttt tctgttatta
     5101 tcagttgcat tgttatatag ctgttccaag caaaatgaga aagctcaaaa taaatatgtt
     5161 aatccaaatg agtatgcaaa gcataaatcg aaaatggatg aacattggga aacatatcac
     5221 ggccaagttt atcatgtgtt ttatcaccca ttaattacag acccaaaagt tgcgtttaca
     5281 ggagatacac accaagctaa gggcaacaat gattggatga ttactgtaag tgaatttaaa
     5341 aaatcattga acgaattata taaaaataat tacatcatcg ttaatccaca tgatgtatac
     5401 gacttatcat ctcatcatgt aaaagccaaa aaaataaaat tgccgaaagg taaaaagcca
     5461 ctaatcatct caattgatga tatgaattac tatagttaca tgagacacca tggttatgct
     5521 gaccgtttag tactcgataa aaataaacat gttgtttctg aaacgacaga ctcaaaaggc
     5581 catgctactt attctgatga caatgatatc gtgccaattt taaataaatt tgtaaaagag
     5641 catccagatt tttctttaaa tggtgaaaaa ggtgttgttg ctttaaccgg ttatgagggc
     5701 gtattaggtt atcgcacgaa tgaattaacg agtaaagatt atacaaagaa taaaaaggca
     5761 gttgaggaag ttgttcgcgc aatgaaacgg gatggatggt catttgcgag tcattcatat
     5821 ggtcacatta attttgaaaa aacatcttta gaaggtatta aacgtgatac taaaagatgg
     5881 aaagatgaag ttgaaccaat tgtaggtaaa acagatatgt ttgtatttcc acatggcgca
     5941 caggatagat atacacaagc gtatgattat ttggtagatg aagcagagtt taagtttatt
     6001 gcaggtgtcg gtccaaacaa ttttacagat atatcagcaa cgaacgttta ccaagatagg
     6061 gttgcgattg atggtctaaa cttatttgaa tttaaatata aactcaaacc gttttttaat
     6121 cctgaaaatg tatatagtaa acaagataga caattcttta aaggtaatcg ggattatgaa
     6181 gaatagttaa atttaataaa aagatagaaa gaaaataact ttac
//
LOCUS       DAHHOJ010000127         6115 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.127, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000127 DAHHOJ010000000
VERSION     DAHHOJ010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.127"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            941..1795
                     /locus_tag="NBH32_002331"
     CDS             941..1795
                     /locus_tag="NBH32_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HCD1515902.1"
                     /translation="MNKTNFGDYLNKSKSRKIYKIFDQNLKREFSYWHFRKLFKSYTR
                     QSDVHELKVCTQNDGTMEQIWIDKANQFGVAVTWKDNSIIGFVMRPLINYDAIKNETT
                     LKYAVPTTDCYEVFWGGDNELYNYHYLYPHQRYAIDLVIKENHKTYKNKGKVNTDYFY
                     FGKDIIAPANGTVEKVANGVQDNSIGSTNESQFLGNYIVIKHAENEYSLIAHLQQYSI
                     IVNEGQNVKYGDIIGKVGNSGNSTEPHIHFQVMNDKNIEACTSLKIRFINNRELIKGD
                     VVCGLQGE"
     gene            complement(1855..4281)
                     /locus_tag="NBH32_002332"
     CDS             complement(1855..4281)
                     /locus_tag="NBH32_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide lyase 8 family protein"
                     /protein_id="HCD1515903.1"
                     /translation="MTYRMKKWQKISTITLLMAGVITFNDSEFRSVDKHQIAVADTNV
                     QTTDYEKLRNTWLNVNYGYNQYDENNDAMKKKFEATEKEATNLLSSMKTESDRKYLWE
                     NSKDLDTKSADMTRTYRNIEKIAEAMKHKNTKLNTPDNKNKVKDALEWLHKNAYGKEP
                     TDKVKELTENFKNKTSRNTNLNWWDYEIGTPRALTNTLILLNGDISSDEKKKYTAPIK
                     TFAPESDKILSSVGQPEQAKGGNLVDIAKVKLLESIIEEDTTMMKESIDAFNKVFTYV
                     QSNATGKERNGFYKDGSYIDHQDVPYTGAYGVVLLEGISQMMPMIKETSFKDSNQNDT
                     TLKSWIDEGFMPLIYKGEMMDLSRGRAISRENETSHSVSATVMKSLLRLSDAMDDSTK
                     AKYKQIVKTSVKSDSSYKQNDYLSSYSDISKMKSLMEDSTISTNDLTQQLKIYNDMDR
                     VTYHNKGLDFAFGLSMTSKNVARYESINNENLKGWHTGAGMSYLYNSDVKHYRDNFWA
                     TADMKRLAGTTTIENEEPKGTDVKKSSKTFVGGTKFDDQHASIGMDFENQDKTLTAKK
                     SYFILNDKIVFLGTGIKSTDSSKNPVTTIENRKTNGYTLYTDDKQTTASDNQDTNSVF
                     LESTNKPKNNIGYHFLNKSKITVKKESHTGKWSDINKSQKSEDKTDEYYEVTQKHSDK
                     DNKYGYVLYPGITKDNFKSKASQVTVVKQDDDFHVVKDNESVWAGVNYSDSAKTFEIN
                     GTKVEVKAKGMFILKKKNDKTYECSFYNPESTNPASDIESKISMTGYSITNKDTSTTN
                     ESGVRFELTK"
     gene            complement(4278..4445)
                     /locus_tag="NBH32_002333"
     CDS             complement(4278..4445)
                     /locus_tag="NBH32_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515904.1"
                     /translation="MRKIWVIDCLYASTFFVDCLTVNSHLYTLTNFEKLNHYLIIIFI
                     AHAQHKKETCQ"
     gene            4689..5105
                     /locus_tag="NBH32_002334"
     CDS             4689..5105
                     /locus_tag="NBH32_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HCD1515905.1"
                     /translation="MKTKEVVALMNISQDTLRYYEKVGVIPPVNRDENGYRIYNDSDL
                     NWIYLVKNLRNAGVSIESLIEFCRLAQLPKNENIQAQQKQILNKQLEELNENLKTIHD
                     ARDLLQYKIDNYDNHIAKINASDNHDANVERLWERK"
     gene            5175..6023
                     /locus_tag="NBH32_002335"
     CDS             5175..6023
                     /locus_tag="NBH32_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1515906.1"
                     /translation="MNHIEISKDVKIPVLGFGVFQIPQEQTAEAVKEAIKAGYRHIDT
                     AQSYLNETEVGQGIEASGIDRSELFITTKVWIENVNYEDTIKSIERSLQRLNLDYLDL
                     VLIHQPYNDVYGSWRALEELKENGKIKAIGVSNFGVDRIVDLGIHNQIQPQVNQIEIN
                     PFHQQEEQVAALQQENVVVEAWAPFAEGKNQLFQNQLLQAIADKYNKSIAQVILRWLV
                     ERDIVVLAKSVNPERMAQNLDIFDFELTEEDKQQIATLEESNSQFFSHADPEMIKALT
                     SRELDV"
BASE COUNT         2089 a          904 c         1002 g         2120 t
ORIGIN      
        1 ttttgtactt caatcttgtt taactttaat attaattcat tgttgtaatt atactcattt
       61 aatgtcatgt tgataaatcc ctgttttctc catcattaaa attttattcg atgcccatct
      121 atttatctaa gtaaatagat aatatcattt tattatttat agcgttttag gttatgtaca
      181 attatatttt cttatttatt aggttggttg tcaatgttgt tggaactttt cagctgatat
      241 gctgtaaaag ctactaaaga taaataatgt aatgaagtat tgacaggata ttatgaggaa
      301 ctagattctt tctatttaaa aatttttagt ttgaaatact tattgatggt aaactgatta
      361 tcgaacacag cgaattgaca gtgactaagt tgcaataaaa atttcaaatt gtaggactga
      421 ccgttatgac taagagaaaa atgtgaatat actttaaaat agaagtaata caactcgttg
      481 tatcaagtat aaatgttact gatgtcttaa tgcgtctttg aattcctatt caaaaaatat
      541 caaagtggtt aactaaatgt tataaaaata gaggaaatgc ttttgcagat aattggaaaa
      601 tgatgtgaat ctatcaaaaa gacttaaagt tttagaagaa catataataa aaaaaggtta
      661 tgcacatctt taataaagaa taaaaagcaa ttttgatttc atagacaaaa acttccacaa
      721 gattcaggca acgtatatgt gtgaggtatt ttgtgtcttt gaaagcggat aggatgatgt
      781 aaaaaaacgt gattattcat tccaacatat tcgacgagaa atgtaaaaaa gcaaattcat
      841 aaaatttcca ggggaagcaa aaaatataag gtagcgtaaa aatgacgcag gaactggaga
      901 aagaattgat accaaaataa acataagagg ttaagaatat atgaataaaa caaactttgg
      961 ggattattta aataaaagta agagcaggaa aatatataag atttttgatc aaaatttgaa
     1021 gcgagagttt agttattggc actttaggaa acttttcaaa tcttatacaa ggcagtctga
     1081 tgtacatgag ttaaaagtgt gtactcaaaa tgatggtact atggagcaaa tatggataga
     1141 taaagcaaat caatttggag tggcagtgac ttggaaagat aatagtatta ttggatttgt
     1201 tatgcgtccg ttaatcaatt atgatgctat taagaatgaa acgactttaa aatacgcagt
     1261 gccgacaaca gattgctatg aggttttttg gggtggagat aatgaattat ataattatca
     1321 ctatttatac ccacatcaaa ggtatgctat tgatttagtt attaaagaaa atcataaaac
     1381 atataagaac aaaggaaaag taaatactga ttatttttat tttggaaaag atatcattgc
     1441 gccagcaaat ggtacagttg aaaaagtagc gaatggggtt caagacaaca gtattggaag
     1501 cacgaatgaa tcacagtttt taggaaatta tattgtaatc aagcacgcag aaaatgagta
     1561 tagcctaata gctcatttac aacaatattc aatcattgtg aatgaggggc aaaatgttaa
     1621 atatggtgac atcattggga aggttgggaa ttctggcaat tctactgaac ctcatataca
     1681 ttttcaagta atgaatgata agaatattga agcatgtaca tctttgaaaa ttcgatttat
     1741 aaataatcga gaacttatca agggtgatgt ggtctgcgga ttacaaggtg aatgatggcg
     1801 atatttataa aatctcgaca ctataaaaat ggtatagtgc cgagattttc ttgcttattt
     1861 agttaattca aagcgtacgc cggattcatt tgtagtcgat gtatctttgt ttgtaataga
     1921 gtatccagtc attgaaattt tagattcaat atctgaagcg ggatttgtag attcgggatt
     1981 atagaagcta cattcataag ttttatcatt tttcttttta agaataaaca ttcctttggc
     2041 tttaacttcg actttagtac cattaatttc aaaagtttta gcgctatcac tataattgac
     2101 accagcccaa accgattcat tatctttcac aacgtggaag tcatcatctt gtttaacgac
     2161 agttacttgg cttgctttgg atttaaaatt atcttttgtt atacctggat acaacacata
     2221 tccatattta ttgtctttat cagaatgctt ttgagttact tcataatact catcagtttt
     2281 gtcttctgat ttttgacttt tatttatatc actccactta ccagtatgac tttctttttt
     2341 tacagttatt ttcgatttgt ttaaaaaatg atagcctata ttgtttttag gtttattggt
     2401 ggactctaaa aagacggaat tggtatcctg attatcggaa gcggttgttt gtttatcgtc
     2461 tgtatataac gtatacccat tcgttttgcg attttcaatc gttgtaactg gattctttga
     2521 tgaatcagta cttttaatgc cagttcctaa gaagacaatt ttatcgttta atatgaaata
     2581 tgattttttg gcagttaaag ttttgtcctg attttcaaaa tccattccga tactagcatg
     2641 ttggtcatcg aattttgttc cgcctacaaa agttttactc gactttttaa catccgtacc
     2701 tttaggttct tcattttcta tagttgtagt acctgctaaa cgtttcatat cggcagtcgc
     2761 ccagaagtta tcacggtagt gtttcacatc gctattgtat aaataagaca ttccagcacc
     2821 agtatgccaa ccttttaggt tttcattatt gatactttcg taacgtgcga cgtttttcga
     2881 cgtcatactt aaaccaaatg caaagtctaa gcctttattg tgataggtga cacgatccat
     2941 gtcattatat attttaagtt gttgtgttaa atcgttagta gaaatagtgc tgtcttccat
     3001 taaagacttc attttgctta tatccgaata agagcttaaa taatcgtttt gtttataact
     3061 tgaatcagat ttaactgaag ttttaacgat ttgtttatat ttagcttttg ttgaatcatc
     3121 catggcatca cttaatctca acaatgattt cattacagtt gcagatactg agtgactcgt
     3181 ttcattttca cggctaatgg ctctaccacg tgataaatcc atcatttcac ctttataaat
     3241 gagtggcata aatccttcat caatccacga ctttaatgtt gtatcatttt gattactatc
     3301 tttaaaagat gtttctttta tcatcggcat catttgagaa ataccctcta agagtacaac
     3361 gccataagca ccagtgtatg ggacgtcttg atgatcaata taagagccgt ctttatagaa
     3421 tccattacgt tctttaccag ttgcattact ttgaacgtaa gtgaagactt tattaaatgc
     3481 atctattgat tctttcatca tagttgtatc ttcttcgata atactttcta aaagttttac
     3541 tttagcaatg tctactaaat ttcccccttt agcttgttca ggttgtccta ctgatgataa
     3601 tattttgtca ctttctgggg cgaaagtttt gataggggca gtgtattttt tcttttcatc
     3661 acttgaaata tcaccattca gtaatattag tgtatttgtt aatgctctag gtgttccaat
     3721 ttcataatcc caccaattta agttggtatt tctagaagtt ttatttttaa aattctcagt
     3781 cagttctttt actttatcag tcggttcttt tccatatgca tttttatgca gccactcaag
     3841 ggcatcttta actttgtttt tattatccgg agtattcaac ttagtatttt tatgcttcat
     3901 cgcttctgcg attttctcaa tattacgata ggtacgagtc atatccgcag acttcgtatc
     3961 taaatctttt gagttttccc acaagtattt cctatcactt tcagttttca tgctacttag
     4021 taaattcgtc gcctctttct ccgtagcctc aaacttcttt ttcattgcat cattattctc
     4081 atcatactga ttataaccgt agttaacgtt cagccatgtg ttcctcaact tttcataatc
     4141 tgtcgtttga acattcgtat cagccacagc gatttgatgt ttatcaacac ttctgaattc
     4201 actatcatta aaagtaatca caccagccat taataacgta atggtagata ttttttgcca
     4261 tttcttcatt ctatatgtca ttgacatgtc tcctttttgt gttgcgcgtg cgcaatgaat
     4321 attatgatta aataatgatt caatttttca aaattcgtta acgtatacaa atgactgttt
     4381 actgtcaaac aatccacaaa gaatgttgat gcatataaac aatcgatcac ccaaattttc
     4441 cgcatcaccc aaaaattgca acgaaaataa tttttaaaaa attaaatatt atcaatatta
     4501 gtgattatat cattttaaat atttaagtta tacaattttt aaagtaatta aaaacgatga
     4561 aatgtaaaat gttaaaaaag agacaaattt aaatttgagt aataaaataa gaagtattca
     4621 aacgaagatg gcaatatata aaccaacata actttagacg accaaacata actgaaagga
     4681 tagtcgcaat gaaaactaaa gaagtcgtag cgctcatgaa tatatctcaa gacactttaa
     4741 gatattatga aaaggttggt gtgattccac ctgttaatcg agatgaaaat ggatatagaa
     4801 tatataatga tagtgattta aattggattt atttagtgaa aaatttgcga aatgcaggcg
     4861 tcagtattga atcgcttatc gaattttgta ggttagcgca gcttcctaaa aatgaaaata
     4921 ttcaagcaca gcaaaagcaa attttaaata agcaactcga agaattaaat gaaaacttaa
     4981 agacaattca tgatgcgaga gacttactac aatataaaat tgataattat gataatcata
     5041 ttgctaaaat caatgctagt gataatcatg atgccaatgt tgaacgtctt tgggagagaa
     5101 agtaaaccta tggcttgcta tagagtttgc tccatggttt atagtgagct tacaagaaaa
     5161 aggaggttca ttttatgaat catattgaaa taagtaaaga tgtaaagata ccagtattag
     5221 gtttcggagt atttcaaatt cctcaagaac aaacagcaga agcggttaaa gaagcaatta
     5281 aagcgggtta tagacatatt gatacagcgc aaagttattt aaatgaaact gaagtaggtc
     5341 aaggtattga agcttcaggt attgatagaa gtgaactatt cattacgact aaagtatgga
     5401 ttgaaaatgt taactatgaa gatactatca aatcaatcga acgatcatta caacgattaa
     5461 atttagacta tcttgatcta gtgcttattc accaacctta taatgatgta tatggctcat
     5521 ggcgtgcatt agaagagtta aaagaaaatg gtaaaattaa agcaatcggt gtatctaact
     5581 tcggcgttga tcgaattgta gatttaggta ttcacaatca aattcaacca caagtgaacc
     5641 aaatagaaat taatccattc catcaacaag aagaacaagt tgcagcactt caacaagaaa
     5701 atgttgtggt agaagcttgg gcaccatttg cagaaggtaa aaatcaatta ttccaaaatc
     5761 aattgttgca ggccattgca gataaatata acaaatcaat tgcacaagtt attttacgct
     5821 ggttagtaga acgtgacatc gttgtactcg caaaatcagt gaatccagaa cgtatggctc
     5881 aaaacttaga tatatttgat tttgaactta cagaagaaga taaacaacaa attgcgacat
     5941 tagaagaaag taatagccaa tttttctcac atgctgatcc agagatgatt aaagcactta
     6001 caagcagaga attagatgtt taatcataat ataacaaagt aaataattta cattacacta
     6061 acctgaaact tggatgttaa agtctatgtt tcaggttatt ttttactctt aaaaa
//
LOCUS       DAHHOJ010000128         6007 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.128, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000128 DAHHOJ010000000
VERSION     DAHHOJ010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 6007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.128"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..1022
                     /locus_tag="NBH32_002336"
     CDS             <1..1022
                     /locus_tag="NBH32_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCD1515907.1"
                     /translation="IFFNEVYGPKWLYIISVIVFSLSYLILVIVNNRLNTLMFYILLI
                     IHYFIICYFVFSVHPMLSLFFFYSAFAVPFTFKNNVKKTATNFFILTMIICTIITYLF
                     YNNYFVAMMVYYVVISLIMLDNFKKMKNREYQKEIAEKNRHINTLIAEQERHRIGQDL
                     HDTLGHVFASLSLKSELAYKLIDADVEKVKAELLAINKLSRESLNKVREIIDDVKLPS
                     FIEEIDSIRKVLKDADIDFTFENKELAQVLSPTKQSMLVMITREAINNVIKHANASKV
                     HGKLKTVNNHKLLLMIEDDGKGIDSDCEVKSISQRVQHLNGTLAVDSTNGTKIIIEIS
                     TGGIA"
     gene            1019..1621
                     /locus_tag="NBH32_002337"
     CDS             1019..1621
                     /locus_tag="NBH32_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCD1515908.1"
                     /translation="MTSLIIAEDQNMLRQAMVQLIKLHGDFEILADTDNGLDAMKLIE
                     EYNPNVVILDIEMPGMTGLEVLAEIRKKHLNIKVIIVTTFKRPGYFEKAVVNDVDAYV
                     LKERSIEELVETINKVNNGEKEYSATLMTSFFVDKNPLTPKEQIVLREIGNGLSSKEI
                     SEKLFLTDGTVRNYTSVIIDKLFADNRFDAWKKANEKGWI"
     gene            complement(1734..1922)
                     /locus_tag="NBH32_002338"
     CDS             complement(1734..1922)
                     /locus_tag="NBH32_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HCD1515909.1"
                     /translation="MNISNENTVTLIAVIIAIIIGIFLQIFFKLPLIVTAVLSILLGI
                     FVGFIVYLIVSFYNKRKN"
     gene            2061..2594
                     /locus_tag="NBH32_002339"
     CDS             2061..2594
                     /locus_tag="NBH32_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCD1515910.1"
                     /translation="MKSNKSLAMIVVAIIIVGVLAFQFMNHTGPFKKGTNHETVQDLN
                     GKDKVHVQRVVDGDTFIANQNGKEIKVRLIGVDTPETVKPNTPVQPFGKEASNYSKKT
                     LTNQDVYLEYDKEKQDRYGRTLAYVWISKDRMYNKELVEKGLAREKYFSPNGKYRNVF
                     IEAQNKAKQQKLNIWSK"
     gene            complement(2737..3591)
                     /locus_tag="NBH32_002340"
     CDS             complement(2737..3591)
                     /locus_tag="NBH32_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515911.1"
                     /translation="MSFYVVLIIIIVALIGILVLNQRYSNSKIDTEVYARKQLIKKNK
                     ALSAENAELRSQMLSSNNDVGHHAYKNAKRELRKILDSYLENGKLKYYDIIVTSNLAT
                     KHPFFEYARSFDFIIVSDIGLINVDVKSWGEKTFYHFDVPDEHDTEMSNSNIEKVVGH
                     YISQQYHDQFNSSRKSIYTFTETVQPNRVIYDFYDYDPYQLAANNAKALKDHIEQKFN
                     FKVQSTGVIYFSDGTVNIIQGSEERDKYVDTVSTKSSLRRIISEAIELSKHPLNKEQV
                     DQITAIFK"
     gene            3922..4497
                     /locus_tag="NBH32_002341"
     CDS             3922..4497
                     /locus_tag="NBH32_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515912.1"
                     /translation="MNAKEQLVDNLMKTSSQLFKFHGEVAMQLFLNDELKLPSIVEIC
                     VERKRLSDIVKVIPQSYALLYIDKQDQAIAKEDLSLSKIAKVYVQYDDTTIMSIFVYD
                     VVNDEWIFRLDPNIRIPKSNIYFHSLNWDVDYIKPEIVLMYDLMQHHQYHHYSNYKRV
                     IDALSYYQFFILKFVVGEQRIKDAIQRTINN"
     gene            complement(4551..5933)
                     /locus_tag="NBH32_002342"
     CDS             complement(4551..5933)
                     /locus_tag="NBH32_002342"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HCD1515913.1"
                     /translation="MKVSKFGGSSVSNATQIKKVLNIVNSDPERKIVIVSAPGKRHDN
                     DIKTTDLLIRLYEKVINHLDYHDKKREIIQRYDDIVKELQMDESILRTIDVTLEHYIN
                     QLKNEPKRLLDALLSCGEDFNAQLIALYNNSQGIPTKYISPKEAGIFVTDVPQEAQIL
                     DSAYSEIKKLRDYEEKLIIPGFFGVSHEGYIVTFPRGGSDITGAIISSGVRATIYENF
                     TDVSGIYKANPNIIKDPELIEEITYREMRELSYAGFSVFHDEALQPLYKDRIPVVIKN
                     TNRPQDKGTFIVHDREINAKNVISGISCDKDFTVINIKKYLMNRQVGFTRKILGVLED
                     NNISFDHMPSGIDTISIVMRSKQIQNKETKVLNEIRQKCDVDELSIEHDLAILMIVGE
                     GMNKVIGTANKITHALAESKINLKMINQGASEISMMFGIHVDDAEKAVLSTYEFCYHG
                     VALKNLCDKN"
BASE COUNT         2155 a          816 c          929 g         2107 t
ORIGIN      
        1 gtatattttt taatgaagta tatggtccca aatggttgta tattatatca gtcattgtct
       61 tttcgttgtc gtatcttata ttagttatag taaataatag acttaataca ttaatgtttt
      121 acattttgtt gattattcat tattttatta tttgttattt tgttttcagt gtacatccaa
      181 tgctaagttt gtttttcttt tatagtgctt ttgccgttcc atttactttt aaaaataatg
      241 ttaaaaaaac ggcaactaat tttttcatac taacaatgat tatatgtaca ataataacgt
      301 acttatttta taacaactat tttgttgcaa tgatggttta ttatgtcgtt atatcgttaa
      361 taatgctaga taattttaaa aaaatgaaaa accgtgaata tcaaaaagaa atagcagaaa
      421 aaaatagaca tattaataca ttaattgctg aacaagagcg acatagaatt ggtcaagact
      481 tacatgatac gttagggcat gtgtttgcaa gtttatcatt aaaatcagaa ttagcttata
      541 aactaataga tgctgatgta gaaaaagtaa aagctgaatt attagcaatt aataaattat
      601 ctcgtgaatc attgaacaaa gttcgagaaa ttattgatga tgtaaaatta ccatcattta
      661 ttgaagagat tgatagtata cgtaaagttt taaaagatgc tgatattgat tttacatttg
      721 aaaataaaga attagcgcaa gtattaagtc ctactaaaca atctatgtta gttatgatta
      781 cgcgtgaagc gataaataat gttattaaac atgcaaatgc ttcaaaagtt catggtaaat
      841 taaaaactgt aaacaatcat aaattactgc ttatgattga agatgatggc aaaggtatcg
      901 atagtgattg tgaggtgaaa agtatttcac agcgtgtaca acatttaaat ggaactttag
      961 cagtcgactc aacaaatgga actaaaataa tcattgaaat ctcaacagga ggaatagcat
     1021 gacatcttta attattgcag aagatcaaaa tatgttacga caggcaatgg ttcaattaat
     1081 taaactacat ggtgattttg aaattttagc agatactgat aatggtctcg atgcaatgaa
     1141 acttattgaa gaatataatc ctaacgttgt tattttagat atagaaatgc caggcatgac
     1201 tggacttgaa gttttagcgg aaattagaaa aaagcatttg aatattaaag tgattattgt
     1261 aacaactttt aaaagaccgg gatactttga aaaagcagtt gtgaatgatg tggatgcata
     1321 tgttttaaaa gaacgttcta tagaagaatt ggtggaaacc attaataaag taaataacgg
     1381 agagaaagaa tatagcgcca cattgatgac ttcatttttt gtagataaaa acccattaac
     1441 gcccaaagaa caaattgtat taagggaaat tggcaatggt ttaagtagta aagaaataag
     1501 tgaaaaatta tttttgacag atggaacagt tagaaattat acatctgtta taattgataa
     1561 attatttgca gataatcgtt ttgatgcttg gaaaaaggca aatgaaaaag gctggatcta
     1621 aatacaaaca aaaaagtatt gagtgttttg atttaagaga cttggaaagt gagtcaataa
     1681 tcaaaatctt actcaatact tttattttgg tactcgcaat ttagatttgt taactaattt
     1741 tttcgcttat tataaaacga aacaatcaag taaacgataa agcctacaaa gatacccaat
     1801 aaaatagata gtactgccgt cactattaat ggtaatttga aaaatatttg taggaaaata
     1861 ccaatgataa ttgcgataat tactgcaatt aatgtgactg tattttcatt tgaaatgttc
     1921 atttattttc actccttaac aataacatta tatcatgcta tagctttcca aaatattgaa
     1981 atatgtagat atggctattg acgatatttc ttaactttta tatgattaat cggaatgaaa
     2041 aaagagaagt aggtggcaat atgaagtcaa ataaatcgct tgctatgatt gtggtagcca
     2101 tcattattgt aggtgtatta gcatttcaat ttatgaatca tacgggtcct ttcaaaaagg
     2161 ggacgaatca tgaaactgta caagatttaa atggtaaaga taaagtacat gttcaaagag
     2221 ttgtggatgg tgatacattt attgcaaatc aaaatggtaa agaaattaaa gttaggctta
     2281 taggggttga tacgccagaa acggtgaaac cgaatacgcc tgtacaacca tttggcaaag
     2341 aagcatcaaa ttatagtaag aagacattaa caaatcaaga tgtttattta gaatatgata
     2401 aagaaaaaca agatcgctat ggtagaacat tggcgtatgt atggataagt aaagatcgta
     2461 tgtacaataa ggaattagtg gaaaagggac ttgctagaga gaagtatttt tcaccaaatg
     2521 gcaaatatag aaatgtattt atagaagcac aaaataaagc taaacaacag aaattaaata
     2581 tttggagtaa ataattaaat aggtgattat atatattaaa gtaaaatata cttgaagcat
     2641 cgatttatta ttaccataaa tttatatata gaaataattt aaaggcataa taaccatagt
     2701 gcgtatcgag cgtaatggat tattatgcct ttgattttac ttgaatatag cagtgatttg
     2761 atcaacctgt tctttattta aagggtgttt agatagctct atggcttcgc taataatacg
     2821 tcgtaacgaa gattttgttg aaactgtatc aacatattta tctctttctt cagaaccttg
     2881 aatgatatta actgtaccat cactaaagta aataacaccg gtactttgga ctttaaagtt
     2941 aaatttttgt tcaatatgat cctttaatgc tttagcatta tttgctgcta attgatacgg
     3001 atcataatca taaaaatcat aaataacacg atttggttga actgtttctg taaaagtata
     3061 gatagacttt cttgatgaat taaactgatc atgatattgc tggctaatgt aatgaccgac
     3121 aactttttca atattactat tgctcatttc tgtatcatgc tcatcaggta catcaaagtg
     3181 ataaaatgtt ttttcacccc agctttttac atcaacgttt attaaaccga tatctgaaac
     3241 aatgataaaa tcaaatgaac gtgcatactc gaaaaacgga tgtttagtag ctaaattgct
     3301 tgtaacgata atatcgtaat attttaattt accattttcg agatagctat ctaaaatctt
     3361 gcgtaattct cttttagcat ttttgtatgc atggtggcca acatcattat tagaactcag
     3421 catttgactt cttaattcag cattttctgc acttaatgct ttgttctttt taataagttg
     3481 ctttcttgca taaacttcgg tatctatttt actattacta tacctttgat ttaaaactaa
     3541 tataccaatt aatgctacaa tgataatgat aagtacaaca taaaaagaca ttttttcacc
     3601 aatccttttt gacttcttta actttgtata caataataat taataaagat taattgttat
     3661 tcaatttccc acatttttat tagttgattt tagttcatca ttgttataat caaattataa
     3721 actgacagat attgatgttc aatgaatatg acgtgaaaga ttcgtgaatt caagtttatg
     3781 tcgaatttat gttataacgg tcatttaaat gacagaatta ggtcactcat agtattttga
     3841 agattgaatt cattaatttt aaaatgtata atgatatttg tgaaagcgct tgcttaggag
     3901 gtgtatttga gagtgaatga aatgaatgct aaagaacaat tagtggacaa tttaatgaaa
     3961 acatcatcgc aattatttaa atttcacggt gaagttgcca tgcagctttt cttaaatgat
     4021 gaattaaaat taccttctat tgttgaaata tgcgtggaac gtaagcgttt aagtgatatt
     4081 gtgaaagtta ttccgcaatc atatgcgtta ctatacatag ataagcaaga tcaagcaata
     4141 gctaaagaag atttatcact ttcaaaaatt gcaaaagttt atgtgcaata tgatgataca
     4201 acaataatga gtattttcgt ttatgatgta gtaaacgatg aatggatttt tagattggat
     4261 ccgaatatac gtatacctaa gagtaacatt tacttccata gtttaaattg ggatgtggat
     4321 tatattaaac cggaaatcgt tctaatgtat gatttaatgc aacaccatca gtatcatcat
     4381 tattccaatt ataaacgtgt catagatgca ttaagctact atcaattttt tattttaaaa
     4441 tttgtagtag gtgagcaacg tattaaggat gcaatccaga gaacaataaa taattaagaa
     4501 aaagcaattc ataacgcagt tgaatacatg tgttacgaat tgcttttata ttagttttta
     4561 tcacacaagt tttttaatgc aaccccgtga tagcaaaact catatgtaga taatacagct
     4621 ttttcagcat catctacatg aattccaaac atcatcgaga tttccgaagc accttggtta
     4681 atcattttta agttaatttt tgattcagct aaggcatgtg taattttatt tgcagtaccg
     4741 atgactttat tcataccttc gccgacaatc attaaaattg ctaaatcatg ctcaatactt
     4801 agctcatcaa catcacattt ttgacgaatt tcatttaata cttttgtttc tttattttga
     4861 atttgttttg aacgcataac gatactgata gtatcaatac ctgaaggcat atgatcaaat
     4921 gaaatattat tatcctctaa gacacctaat atctttctag taaagccgac ttgtctattc
     4981 attaaatact tcttgatatt aataacagta aaatctttat cacaacttat accgctaatc
     5041 acatttttcg catttatttc tctatcatgc actataaatg tacctttatc ttgagggcgg
     5101 ttcgtatttt taatcaccac agggatgcga tctttataaa gtggttgtaa ggcttcatca
     5161 tggaaaacac taaaaccagc ataagataat tcacgcattt ctcgataagt gatttcttcg
     5221 attaattcag gatctttgat gatatttgga ttagctttat aaataccaga cacatcggtg
     5281 aaattttcat aaattgtagc tctaacacca cttgatatga tggcgccagt tatatctgat
     5341 ccgccacgtg gaaatgtaac tatatatcct tcatgagata cgccaaaaaa tcctgggata
     5401 attagtttct cctcataatc tcttaatttt ttaatttcag agtaagcact atctaatatt
     5461 tgtgcttctt gtgggacgtc agtaacaaaa atacccgctt ccttcggtga tatatatttt
     5521 gttggtatac cttgactatt attatataaa gctatcaatt gcgcattaaa atcttcacca
     5581 caagaaagta atgcatctaa tagtctcttt ggttcatttt ttaattgatt aatataatgt
     5641 tccaaagtca catctatcgt ccgtaaaata ctttcatcca tttgcaattc ttttacaata
     5701 tcatcataac gctgaataat ttctcttttt ttatcatgat aatcaagatg attaatgacc
     5761 ttttcatata atctgattaa caaatcagtt gttttaatat cattatcatg tcttttacct
     5821 ggagcagaaa cgataacaat ctttcgctct ggatcagaat taacaatatt taaaaccttt
     5881 ttaatttgag tagcattgga gacggagcta ccaccgaatt tagaaacttt catagtgcat
     5941 gccaaccttt ctaaaaaatc agaaaattgt attcagtttt gtaagtaaga atgatattat
     6001 gtattta
//
LOCUS       DAHHOJ010000129         5950 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.129, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000129 DAHHOJ010000000
VERSION     DAHHOJ010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5950
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.129"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            59..823
                     /locus_tag="NBH32_002343"
     CDS             59..823
                     /locus_tag="NBH32_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCD1515914.1"
                     /translation="MDINSEEYKQEVLIKDVVMLAARILLESGAEGTRVEDTMTRIAK
                     KLGYSESNSFVTNTVIQFTLHSESFPRIFRITSRDTNLIKISQANKISRQITNNEISL
                     AEAKTQLEKIYVAKRDSSLPFKGFAAAMIAMSFLYLQGGRLIDVLTAILAGSLGYLVT
                     EILDRKLHAQFIPEFIGSLVIGIIAVIGHTLIPTGDLATIIIAAVMPIVPGVLITNAI
                     QDLFGGHMLMFTTKSLEALVTAFGIGAGVGSVLILV"
     gene            836..1288
                     /locus_tag="NBH32_002344"
     CDS             836..1288
                     /locus_tag="NBH32_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HCD1515915.1"
                     /translation="MFWILNFIFSFLASMFFCVIFDAPRKLYLSCGFVGTCGWMVYTL
                     FFNGFNVHTIYSSFFGSLALGLLSHYMARKQKEPAIIFMVTGIIPLVPGGLAYDATKN
                     LVLLNFSTAINTMLEVTLIAGAIALGLLFADQISKLIVSGFVKSFKRL"
     gene            complement(1429..2913)
                     /locus_tag="NBH32_002345"
     CDS             complement(1429..2913)
                     /locus_tag="NBH32_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HCD1515916.1"
                     /translation="MEKKNKQIDRGDLKQNLSEKFVWAIAYGSCIGWGAFILPGDWIK
                     QSGPIAASIGIVIGALLMILIAVSYGALVERFPVSGGAFAFSFLSFGRYVSFFSSWFL
                     TFGYVCVVALNATAFSLLVKFLLPDVLNNGKLYTIAGWDVYITEIIIATVLLLVFMLV
                     TIRGASVSGSLQYYFCVAMVIVVLLMFFGSFFGNNFALENLQPLAEPSKGWLVSIVVI
                     VSVAPWAYVGFDNIPQTAEEFNFAPNKTFKLIVYSLLAASLTYVVMILYTGWLSTSHQ
                     SLNGQLWLTGAVTQTAFGYIGLGVLAIAIMMGIFTGLNGFLMSSSRLLFSMGRSGIMP
                     TMFSKLHSKYKTPYVAIIFLVGVSLIAPWLGRTALTWIVDMSSTGVSIAYFITCLSAA
                     KLFSYNKQSNTYAPVYKTFAIIGSFVSFIFLALLLVPGSPAALTAPSYIALLGWLIIG
                     LIFFVIRYPKLKNMDNDELSRLILNRSENEVDDMIEEPEKEKTK"
     gene            complement(3034..3402)
                     /locus_tag="NBH32_002346"
     CDS             complement(3034..3402)
                     /locus_tag="NBH32_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCD1515917.1"
                     /translation="MKLFIILGALNAMMAVGTGAFGAHGLQGKISDHYLSVWEKATTY
                     QMYHGLALLIIGVISGTTSINVNWAGWLIFAGIIFFSGSLYILVLTQIKVLGAITPIG
                     GVLFIIGWIMLIIATFKFAG"
     gene            complement(3535..3915)
                     /locus_tag="NBH32_002347"
     CDS             complement(3535..3915)
                     /locus_tag="NBH32_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5327 family protein"
                     /protein_id="HCD1515918.1"
                     /translation="MVNRETLIARIEQELVQAEQAQHDHDFEKHMYAIHILTSLYAST
                     SNTPHIGEQQMNRRIANHNQMPQSQITQPTHQVTAAEIEAMGGKVNTHSAHHHNKSYS
                     QPSNQQQRLATDDDIGNGESIFDF"
     gene            complement(3916..4572)
                     /locus_tag="NBH32_002348"
     CDS             complement(3916..4572)
                     /locus_tag="NBH32_002348"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCD1515919.1"
                     /translation="MEWSQIFHDITTKHDFKVMHDFLEKEYSTAIVYPDRENIYQAFD
                     LTPFENIKVVILGQDPYHGPNQAHGLAFSVQPNAKFPPSLRNMYKELADDIGCVRQTP
                     HLQDWAREGVLLLNTVLTVRQGEANSHRDIGWETFTDEIIKAVSDYKEHVVFILWGKP
                     AQQKIKLIDTSKHCIIKSVHPSPLSAYRGFFGSKPYSKANAYLESVGKSPINWCESEA
                     "
     gene            4756..5586
                     /gene="thiD"
                     /locus_tag="NBH32_002349"
     CDS             4756..5586
                     /gene="thiD"
                     /locus_tag="NBH32_002349"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCD1515920.1"
                     /translation="MALKKVLTIAGSDTSAGAGMQADLKTFQELDTYGMVALTAIVTM
                     DKDTWSHDVTPLPMDVFEKQLETALSIGPDAIKTGMLGTEEIIKRAGEVYEASNAQYF
                     VVDPVMVCKGEDEVLNPGNTEAMIKYLLPKATVVTPNLFEAGQLSGLGKLNSIEDMKK
                     AATIIFDKGAQHVIIKGGKALDQDKSYDLYYDGQTFYQLTTDMFQQSYNHGAGCTFAA
                     ATTAYLANGKSPKEAVISAKAFVASAIKNGWKMNDFVGPVDHGAYNRIEHIDVEVTEV
                     "
BASE COUNT         1951 a         1074 c          975 g         1950 t
ORIGIN      
        1 tattttatag tgtataaact ataatgagta ttaaattctt ataaacaatg gtgatgaaat
       61 ggacataaat tcagaagaat acaaacaaga ggtacttatc aaagacgttg tcatgcttgc
      121 tgctcgcata ctattagaat ctggtgcaga aggtacgcgt gtagaagata ccatgacacg
      181 tattgcaaaa aaacttggtt acagtgaaag taacagcttt gttacaaaca ctgtcatcca
      241 gtttacgtta cattcggaat cgtttcctag aatatttaga attacctctc gagatacaaa
      301 cttaataaaa atttctcaag ctaataaaat ttcgcgtcaa attacaaaca atgaaatttc
      361 tttagccgaa gcaaaaacgc aacttgaaaa aatatatgtt gctaagcgtg acagcagtct
      421 tccctttaaa ggttttgctg cagcaatgat tgcaatgagt ttcttatatt tacaaggtgg
      481 tagattgatt gatgttttaa ctgcgatatt agcaggtagt ctaggatacc tagtcactga
      541 gattttagat cgcaagttac atgcacagtt tatcccagaa ttcattggtt cattagttat
      601 tgggattatc gccgttattg gacatacact tatcccaaca ggtgacttgg caactattat
      661 cattgcggca gtcatgccta ttgttcctgg tgtattaata acaaacgcaa tacaagattt
      721 atttggtgga cacatgttga tgttcacaac gaaatcatta gaagcattgg ttactgcgtt
      781 tggcatcggt gctggcgttg gtagcgtatt aattttagta taggagtatc agactatgtt
      841 ttggatctta aactttatct ttagcttttt agcttcaatg ttcttctgcg tcatttttga
      901 tgcacctaga aaattatatc tatcatgtgg tttcgttggt acatgtggat ggatggttta
      961 caccttattc ttcaacggct ttaatgtgca cactatatac tctagtttct ttggtagttt
     1021 agcattaggc ttgttaagtc attatatggc tcgtaaacaa aaagaacctg ccatcatttt
     1081 tatggtaacg ggtatcattc cattagtacc tggtggatta gcttacgatg caacgaaaaa
     1141 tttagtctta ttaaatttca gcacagcaat aaatacaatg ttagaagtaa cacttattgc
     1201 aggcgccatc gcattaggtt tattattcgc cgaccaaatt tccaaattaa ttgtttctgg
     1261 gttcgtgaaa tcttttaaac gattataaat attgttagaa atggctcagc ccaaactctt
     1321 agcaattata tataaggttc agctgcctcg tcttaaatgt caatttataa tacaaaaaat
     1381 cgcacgatac gcctccgaat gaagaaggta tattgtgcga ttcttttatt atttagtttt
     1441 ttctttttca ggttcttcaa tcatatcatc aacttcattt tcacttctat ttaaaatcaa
     1501 gcgacttaat tcatcattat ccatattttt caatttagga tatcgaatca caaagaatat
     1561 taaaccgatg attaaccatc caagtaatgc aatataagac ggtgcagtca gtgctgcagg
     1621 agaacctggc actaataaca acgctaagaa aatgaatgat acaaatgagc cgataatagc
     1681 aaacgttttg taaaccggtg catacgtatt actttgtttg ttataactga ataatttcgc
     1741 tgcagacaaa catgtaataa agtaggcaat ggatacacct gtagatgaca tatctacaat
     1801 ccaagtcaat gcagttcttc ctagccaagg tgcaattaac gacacaccta ctaggaatat
     1861 gattgcgaca tatggtgttt tgtatttact atgtaattta ctaaacattg ttggcataat
     1921 acctgaacgt cccatagaaa ataacaagcg acttgaactc atcaagaatc catttaaacc
     1981 agtaaatata cccatcataa ttgcaattgc taatacacct aatccaatat aaccaaatgc
     2041 tgtttgtgta acagcacctg ttaaccacaa ctgcccattt aaactttgat gacttgttga
     2101 taaccaacca gtgtataaaa tcatgacaac ataagttaat gatgctgcta ataaactgta
     2161 cacgataagc ttaaatgtct tgtttggtgc aaagttaaac tcttctgctg tttgtggaat
     2221 attatcaaat ccaacatatg cccatggtgc cacggataca ataaccacaa tagacactaa
     2281 ccatcctttg ctaggttcag ctaacggttg taaattttca agtgcaaaat tattaccaaa
     2341 gaatgaacca aagaacatca ataatacgac gattaccatc gccacacaga aataatattg
     2401 taatgatcca gatacacttg cgccacgaat cgttactagc atgaatacaa gtagtaatac
     2461 ggtcgcaata atgatttccg taatataaac gtcccagccc gcaatggtgt atagtttccc
     2521 attatttaag acatctggca ataagaattt aactagtaaa ctgaatgcgg tcgcatttaa
     2581 agcaacgaca cagacataac caaaagttaa aaaccatgat gagaagaaac tcacatatct
     2641 gccgaaactt aagaaactaa aggcaaacgc gccccctgat actggaaatc tctctactaa
     2701 tgcgccataa ctaaccgcaa ttaatatcat taataatgca ccaataacaa taccaattga
     2761 tgctgcaatc ggacctgact gcttaatcca gtctcctggt aagatgaatg cgccccatcc
     2821 gatacatgaa ccatatgcaa tcgcccatac aaacttttca gataggtttt gttttaaatc
     2881 gcctctatct atttgcttat tctttttttc cataaataaa aactcacctc gaaggtattc
     2941 tatacccaaa agatgagttt gtaactcttt ttcttgatta tttttattaa aaatttaatg
     3001 tttagttaca taggtaatct aaagttttaa aatttaacca gcaaatttga atgtcgcaat
     3061 gattaacatt atccatccaa tgatgaacaa tacgccacca attggcgtaa tcgcacctaa
     3121 aactttaatt tgagttaata ctaaaatata taatgatcca ctaaagaaaa taataccagc
     3181 aaatattaac cagccagccc agttaacatt gattgaagtt gtaccactaa ttacacctat
     3241 aattaataat gctaagccat ggtacatttg atacgtcgtt gctttttccc atactgataa
     3301 atagtgatca cttatttttc cttgtaaacc atgcgcacca aatgcacctg ttccgacagc
     3361 catcatcgcg tttaatgcac ctaaaataat aaataatttc attgctacct ctccttatat
     3421 aattcagtat taataaatgt ttaaatccga acactcgaat ataagtatat gtgtcttgat
     3481 tattaaccta catacttaac aagctattaa gtaattatgt ttcattgttg ctttttaaaa
     3541 atcaaatatg gattcaccat tgccaatgtc atcatctgtc gctaatcttt gttgttggtt
     3601 tgaaggttgt gaatatgact tattatgatg atgtgctgaa tgcgtattta ctttaccacc
     3661 catcgcttca atttcagcag ctgtcacttg atgagttggc tgcgttattt gtgattgtgg
     3721 catttgatta tggttagcaa tacgacggtt catttgttgt tcaccaatat gtggtgtatt
     3781 tgatgttgaa gcatataaag atgttaatat atgtatggca tacatatgtt tttcaaagtc
     3841 atggtcatgc tgcgcctgct ctgcttgtac taattcttgc tcaattcgtg ctattaaagt
     3901 ttctctattc accatctatg cctcactttc acaccaatta attggtgatt ttcctactga
     3961 ctctaaatag gcattcgctt tggaatacgg ttttgatcca aagaatcctc tatatgcaga
     4021 cagtggacta ggatgcactg attttataat acaatgttta gatgtatcga taagctttat
     4081 tttttgctgt gcaggtttcc cccacaaaat aaagacaaca tgttctttat aatcagacac
     4141 tgctttaata atttcatcag taaatgtttc ccaaccaata tcacgatgag aatttgcttc
     4201 accctgtctt acggttaaaa ctgtattcaa taacaagacg ccttctcttg cccaatcttg
     4261 taaatgcggt gtttgtctaa cacatccaat atcatctgct aattctttat acatattacg
     4321 taaagatgga gggaattttg cgttaggttg cactgaaaat gctaatccat gtgcttggtt
     4381 tggaccatga tacgggtctt gtcctaatat aacaactttg atattttcaa acggtgttaa
     4441 atcaaacgct tgatatatat tttccctatc agggtatacg attgcagtcg aatattcttt
     4501 ttctaaaaaa tcatgcataa ctttaaagtc atgtttcgtt gttatgtcat gaaaaatttg
     4561 cgaccattcc attcaatctc acctcaggac atattttaac atatcacact tatctaaagt
     4621 gaatgacatt ttcaaaagtt acaatagtat gaatgattca tttcaacaat catcaaatat
     4681 tgaatgctta atatttttca attaggttaa aaatgatatg atgaatacaa tattattatg
     4741 taggagtgaa gctcaatggc tttaaagaaa gttttaacaa ttgccggttc tgacacaagt
     4801 gctggcgcag gtatgcaggc agatttaaaa acatttcaag aattagatac gtatggtatg
     4861 gtcgctttaa ctgccatcgt tactatggat aaagatacat ggtcacacga tgtcacacca
     4921 ttacctatgg atgtatttga aaagcaactt gaaacggctt tatcaatcgg acctgatgct
     4981 attaaaacag gtatgttagg tactgaggag ataatcaaac gtgctggaga agtatatgag
     5041 gcatctaatg cacaatattt tgtagtagat cctgtcatgg tttgtaaagg cgaagatgaa
     5101 gtgcttaatc ctggaaatac tgaggcgatg attaaatatt tactacctaa agcaacggta
     5161 gtgacgccaa acttatttga agcgggtcaa ttatcaggat taggtaaatt aaattcaatt
     5221 gaagatatga aaaaggctgc aacaattatc tttgataaag gcgcacagca tgtcatcatt
     5281 aaaggtggca aagctttaga tcaagataaa tcgtatgact tatactatga tggtcaaaca
     5341 ttttatcaac taacaacaga catgttccaa caaagttata accatggtgc tggttgtaca
     5401 tttgctgcgg caacaactgc atatttagct aacggtaagt caccgaaaga agctgtgatt
     5461 agcgcgaaag ctttcgtcgc ttctgctatt aaaaacggtt ggaaaatgaa tgattttgtt
     5521 ggtcctgtgg atcacggtgc atacaaccgt attgaacata tcgatgttga agtaacagag
     5581 gtttaacgat taggcggttc atctataaat caacattatt agccacacca cttaaattgg
     5641 gtatgaactc aatttatgtg atgtggttat ttttttataa tcatcatgct ctgtatataa
     5701 tttgaatttg aaagttacat ataagcgtta ttagctttta ttgaaaattt catacatatc
     5761 gaacaataca tacaaatatt tcctatacat ctacatctat gactaaataa ccctttattt
     5821 tcaagatgaa cttaaactca tctatatgct caatacctct atcattatca ttcttttgcg
     5881 atagatgcct ctctaatcat atatgttata tttcccaaat gactgccagg cgactcatta
     5941 aatatatatt
//
LOCUS       DAHHOJ010000130         5934 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.130, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000130 DAHHOJ010000000
VERSION     DAHHOJ010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5934
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.130"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..77)
                     /locus_tag="NBH32_002350"
     CDS             complement(<1..77)
                     /locus_tag="NBH32_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCD1515921.1"
                     /translation="MYVENSYLSKQLCFLFYVSSKEIIK"
     gene            474..839
                     /locus_tag="NBH32_002351"
     CDS             474..839
                     /locus_tag="NBH32_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3139 domain-containing protein"
                     /protein_id="HCD1515922.1"
                     /translation="MKKKKNVIKVIIILFISLIVAVAFFFGLKTYQGHKNIQLIDSYL
                     EEKSLKDKIKSEKTEYSAKKGLFYKEVTFKDEPGVTYVVQPISTNKGLFVEGFDTETK
                     KSLKTAKHKYFNQNYKPSK"
     gene            1021..2298
                     /locus_tag="NBH32_002352"
     CDS             1021..2298
                     /locus_tag="NBH32_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCD1515923.1"
                     /translation="MALFKRKISLPMQVVIALVLGVVVGLLLYGQENVANYIKPFGDV
                     FLNLIKMIVIPVVFCSLALSISNVGESKTVGRYGWKTILYFEIITTIAIGLGIIFGNL
                     FKPGAGLDPTKLPKGDISKYQSTAHAAEQSTYGNHFIDTIVHIIPTNFFEALNKGELL
                     PIIFFAVFFGLGLAAVGKKAEPVKEFLSGSLEAVFWMINKILKLAPLGVFAFICTTII
                     TFGASALLPLLKLVLVVVFAMVFFVFAILGLVAWMCGINIMNIIRILKSELLLAFSTS
                     SSEAVLPVMMKKMENFGSPKEITSFVIPIGYTFNLDGSALYQSIAALFVAQMYGMHLT
                     LSEQIVLMLTLMITSKGMAAVPGTSIVVLLTTLGAMGLPAQGLALIIGVDRILDMVRT
                     CVNVIGNALSTIVIAKWENVYDKAKGQEYLKSI"
     gene            complement(2858..3214)
                     /locus_tag="NBH32_002353"
     CDS             complement(2858..3214)
                     /locus_tag="NBH32_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4889 domain-containing protein"
                     /protein_id="HCD1515924.1"
                     /translation="MKNKKGFGLGISLIAIMLIVCIVLVIMMMTGGKKDTYYGIMKDN
                     ATIEKMISEKDESIEKNVKLPSDADVSVKKGDFVIVYKLADSDKIVKVKKVDHDDVPH
                     GLMMKIHDMGKMHMKH"
     gene            complement(3492..3914)
                     /locus_tag="NBH32_002354"
     CDS             complement(3492..3914)
                     /locus_tag="NBH32_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HCD1515925.1"
                     /translation="MSNSQAIQAIENVLATSKVGVLSTAYNNKPNSRYMVFYNDGLTL
                     YTKTNIHSAKVKEIKDNPAAYVLLGYNDTTNRSFVEMEATIEIVTEQEVIDWLWETQD
                     KSFFSSKEDPELCVLRVVPQSIKLMNDKSLDTPIKIDL"
     gene            complement(3985..>5934)
                     /gene="rsp"
                     /locus_tag="NBH32_002355"
     CDS             complement(3985..>5934)
                     /gene="rsp"
                     /locus_tag="NBH32_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator Rsp"
                     /protein_id="HCD1515926.1"
                     /translation="GDIYNINHGENIIELMIPVFYFHQQDDDFFNGYLDRHLLQSSNY
                     IKSLIADLISTPTSSSLMGKNIGQSIIDTLLKEAFIRIDHEYLPNIALSNPVFIDCVN
                     YIHDNIDAHLSLKDIAMHCNISESYCSNLFVRYLSMNFKDYFTSIKLVNAINLLLSTK
                     HSITTVSELAGFNSHTNFANQFKNYLHFSPKQFRSLVSKITEPPQIHFQQDNVSQFTE
                     LISTIDLTAQLATNTTDIHIDDFNPKDRSQRAKVFVRFSNFNELFQFIFNEYYDINFE
                     HLPKPVVFIDDIHDIEISQTNYNLLNRCFEKLFEKNIGLAIAIKSTQQFETMKQLILT
                     FLQGNQDYKTSKKLVKFMLVFCSNSMTAEEIHLCHLKIKNKNKEIKYSVTVDGFLETY
                     STVEQVYDVMQRLKFHYYFIDIENSKTATHLITKNQHYHQTDTHFEQYKKFILDSGIS
                     STQFVYNNLSVSGFKYTNDGKNPIQLSDIVYHLIALLRYGGGISYQLLDDHSNYISLY
                     NKYGSPLPLMHLYKMFRPFVNEDIEITNNYVLSRKDNNYHFLLFNKINDRYMSDVKQD
                     FIFHNELPQDSLMIIKTLNHEHGSIQHLLPISDQLVYIEKEILDELDKTNYPKTELAV
                     QEETGRTFELKLNHDEVKYICFKPS"
BASE COUNT         2023 a          853 c          968 g         2090 t
ORIGIN      
        1 tttttaatta tttcttttga agaaacataa aataaaaaac acaactgttt gctaagatag
       61 ctgttttcta catacatcca atcactcctt gttaaaataa acaatattat gaagtgtaat
      121 agttgtcaag tactgatttc atcgcaatta tcgaattgtc tataattgaa tttaaatgtt
      181 aaataatgca aaacatatca atatcttagt attttcatcg gtaatgactg atatgactga
      241 ttttttaaat tgaaaaaaat ggaattgtat cattcttaat tgttaaaagt agtcaattat
      301 tttgatagat agtaaaatga aatttgttat taaatttttt caaagcatcg tgtatgtaat
      361 tcgatttcta taataatatt gagaagaaaa aaattgttac aatctggtaa aaactcttta
      421 ttaaataaat tgattcgttc atactgaata gataagcaaa aaggagttac ataatgaaga
      481 agaaaaaaaa cgtgattaaa gttatcatta tattatttat ttcattaatt gttgctgtag
      541 catttttctt tggtctgaaa acatatcaag gccataagaa tattcaactt attgacagtt
      601 acttggaaga gaaaagtcta aaagataaaa ttaaatcaga aaaaacagag tatagtgcta
      661 aaaaaggtct cttctataag gaagttacat ttaaagatga acctggtgta acttatgttg
      721 tgcaacctat cagtactaat aaaggattat ttgttgaagg ctttgataca gaaactaaga
      781 aaagtttgaa aactgcaaag cataaatatt tcaatcaaaa ttataaacca tcaaaatagt
      841 gaagcgtata actggtataa cagtttagtg ccttcaattt taattatttg aggtaaacag
      901 tttttgtatg gaaattagtg atttttgaca tctataaaag ataaatattt taatttttta
      961 tagataatta tcaattttct gatataattg agtgatgtca gatgaagggg agtttgtact
     1021 atggctctat tcaagagaaa aattagccta cctatgcaag ttgttattgc attagtgtta
     1081 ggtgttgtcg taggactttt attatatgga caagaaaacg tagcaaatta cattaaacca
     1141 tttggtgacg tatttttaaa tttaattaaa atgatcgtta taccagttgt attttgctcg
     1201 ctagcgcttt ctatttcgaa cgttggggaa tcgaaaactg tagggcgtta tggctggaaa
     1261 acaattttat actttgaaat tattacaaca atcgcaatag gtttagggat tatcttcggt
     1321 aacctattta aaccaggtgc tggattagac ccaacaaaat tacctaaagg tgatatttct
     1381 aaatatcaat caactgcaca tgcagcagaa caatctacat atggaaatca ttttattgat
     1441 accattgtac atattattcc gacaaacttt tttgaagctt taaataaggg tgaattatta
     1501 cctattatct tcttcgcagt attctttgga ttaggattag ctgcagtagg taaaaaagca
     1561 gaaccagtta aagaattttt aagcggatcg cttgaagctg tgttctggat gattaataaa
     1621 attttaaaat tagcaccact tggagtgttt gcattcattt gtactacaat tattacattt
     1681 ggtgcatccg cattattacc actattaaaa ttagtattag ttgttgtctt tgcaatggtg
     1741 ttctttgtat tcgctatact aggactagtt gcatggatgt gtggtattaa tatcatgaat
     1801 attattagaa tcttgaaaag tgaattgctt ttagcatttt ctacatcaag ttcggaagct
     1861 gtacttcctg taatgatgaa gaaaatggaa aacttcggtt ctccaaaaga aattacttct
     1921 tttgttatac caattggtta tacgtttaac ttagacggat cagcacttta tcaatctatt
     1981 gcagcattat tcgttgcaca gatgtatgga atgcacttaa cattatcaga gcaaattgtg
     2041 ttgatgttaa cattaatgat tacgtctaaa ggtatggcgg cagtaccagg aacttcaatc
     2101 gttgttttat taacaacatt aggtgccatg ggcttaccgg cacaaggttt agcattaatt
     2161 attggtgttg accgtatctt agatatggta cgtacatgtg taaacgttat tggtaacgca
     2221 ttatcaacaa tcgttatagc taaatgggaa aacgtatatg acaaagcaaa aggtcaagaa
     2281 tatttaaaat caatttaaaa aatactatct gacatttaaa gccccttaca acctttggtt
     2341 gttaggggct tttatgcatg cgctaaagcg caggcgaacc gaaacgaagt gacctcaggt
     2401 tgttaggggc ttttatgcat gcgcaaagcg caggcgaacc gaaatgaagg caacctcagg
     2461 ttgttagggg cttttatgca tgcgcaaagc gcaggcgaac cgaaatgaag gcaacctcag
     2521 gttgttaggg gcttttatgc atgcgctaaa gcgcaggcga accgaaacga agtgacctca
     2581 ggttgttagg ggcttttatg catgcgctaa agcgcaggcg aaccgaagcg cagtgacctc
     2641 aggttgttag gggcttttat gcatgcgcaa agcgcaggcg aaccgaaacg aagtgacctc
     2701 aggttgttag gggcttttat gcatgcgcaa agcgcaggcg aaccggaaat agactgacac
     2761 agtaattctt aaaaagcagc agtaagataa ttttcaatta gaaaacatct tactgctgct
     2821 tttaatatct tatatattat tcaaaattaa agcttaatta gtgtttcatg tgcattttgc
     2881 ccatgtcatg aattttcatc attaaaccat gtggtacatc gtcatgatca acttttttta
     2941 ctttaacaat tttatcagaa tctgctaatt tataaacaat tacaaaatca ccttttttaa
     3001 ctgaaacgtc tgcatctgaa ggtaatttaa cgttcttctc aatgctttca tctttttcac
     3061 taatcatttt ttcaattgta gcattgtctt tcataattcc ataatatgta tctttctttc
     3121 caccagtcat catcatgatt actagtacaa tacatacaat caacatgatg gcaattaaac
     3181 taataccaag accaaaccct tttttatttt tcacggttca tcaatctcct taaatgtagt
     3241 taattctgtt atacctaaaa gtacaacagt ttgcaaatgc agtatttata ttacataaaa
     3301 ttaatttaaa aagacacacc ttaatcacaa taaatgtgaa taaaatgtgt ctaaaatatt
     3361 gttatataat aaaaatatag ttaaaagtat taaacgcttt caaaatcgaa atatatataa
     3421 ataggatact gttgaaattt atagctaaca tgttcaattg ctggctaatt ttaaaactaa
     3481 aaacttacat attataaatc aattttaata ggtgtatcta atgatttatc attcattagc
     3541 ttaatggatt gcggaactac tcttaaaaca cataactctg ggtcttcttt tgaactgaaa
     3601 aagcttttgt cttgtgtttc ccatagccaa tcaatcactt cttgttctgt aacgatttcg
     3661 atggtcgctt ccatctcaac gaaactacga ttagttgtgt cgttatagcc taacaaaaca
     3721 tatgctgctg gattatcttt aatttctttg accttagcag aatggatatt cgttttagta
     3781 tataaagtaa gaccatcatt ataaaagacc atatatctac tattaggttt gttattatat
     3841 gcagttgata atacaccaac ttttgacgtt gctaacacgt tttcaattgc ttgaattgct
     3901 tgtgagttac tcatcctaca tcatctcctt atgatataaa tagcctttaa cgtaagaaac
     3961 taaacacaag aggactgtta gtatttagct tggtttaaag caaatatatt taacctcgtc
     4021 gtgatttaac ttgagttcaa atgtacgacc agtttcttct tgaactgcaa gctccgtttt
     4081 agggtaattc gttttgtcta attcatctaa aatttctttt tctatataaa caagttgatc
     4141 gcttattgga agcaaatgtt gaattgaacc atgttcatga ttcaatgttt taataatcat
     4201 caaagagtct tgaggtaatt cattatggaa aatgaaatct tgttttacgt ctgacatata
     4261 tcgatcatta attttattga ataataagaa atggtagtta ttatctttac gactcaatac
     4321 ataattattt gtaatttcaa tgtcttcgtt aacaaaaggt ctaaacattt tatataaatg
     4381 cattaatggt agggggctac catatttgtt gtacaaggaa atataatttg aatggtcatc
     4441 taataattga tagctaatgc caccgccata tcgtaataat gcgattaagt gatacactat
     4501 gtcagataat tgaattggat tcttaccatc attagtatat ttaaacccgc ttacagacag
     4561 gttattatat acaaattgtg ttgatgatat acctgaatct aaaataaatt tcttatactg
     4621 ttcaaaatgt gtgtcagttt gatgatagtg ttgattttta gtgataagat gtgttgcagt
     4681 ttttgaattt tcaatatcaa taaagtaata atggaattta agacgctgca tgacatcata
     4741 aacttgttcc acggttgaat aagtttctaa aaagccatcg actgtcacac tatatttaat
     4801 ttctttattt ttgtttttaa ttttaagatg acataaatga atttcttctg ctgtcataga
     4861 attagagcaa aatacgagca taaatttaac taatttcttg cttgtcttat agtcttgatt
     4921 gccttgtaaa aatgttagta ttaattgctt catcgtttcg aattgttgtg ttgatttaat
     4981 agcgattgct aaacctatat ttttttcgaa aagcttttca aaacatctat ttaacaggtt
     5041 gtaattcgtt tggctaatct caatatcgtg aatatcgtca atgaaaacga caggttttgg
     5101 taagtgctca aagttaatat cgtaatattc attgaaaata aattgaaata gttcattgaa
     5161 attactgaat cgaacaaata ctttggcacg ctgacttcga tctttcggat taaaatcatc
     5221 tatatgaata tctgttgtat ttgtagccaa ctgagctgtt aaatcaattg ttgaaattaa
     5281 ctcagtgaat tgtgatacgt tatcttgttg gaagtgtatt tgtggtggtt cagtaatctt
     5341 ggaaacgagt gaacggaact gtttaggact aaaatgcaag taattcttaa attgatttgc
     5401 aaaatttgta tgactgttaa aaccagccaa ttcagaaaca gttgtaatag aatgttttgt
     5461 agaaagtaat aaattgatgg cgttaacaag tttaatactt gtaaaataat ctttaaaatt
     5521 catactcaaa tatcgaacaa aaagattaga acaatatgat tcagagatgt tgcaatgcat
     5581 cgctatatct tttaaagaca agtgcgcatc aatattgtca tgaatatagt ttacacaatc
     5641 tataaacact ggattgctta aagcaatatt tggtaaatat tcatgatcta ttcttataaa
     5701 agcttcttta agtaaagtgt ctataatact ttgacctata ttttttccca ttaaagaaga
     5761 acttgttggt gtactgatta aatcggcaat taatgactta atataattac ttgattgtaa
     5821 taaatgacga tctaaataac cattaaagaa atcatcatct tgttgatgaa aataaaaaac
     5881 gggtatcatt aattcgatta tattttcgcc gtggttaata ttataaatat cacc
//
LOCUS       DAHHOJ010000131         5808 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.131, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000131 DAHHOJ010000000
VERSION     DAHHOJ010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5808)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5808)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5808
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.131"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            103..450
                     /gene="sarR"
                     /locus_tag="NBH32_002356"
     CDS             103..450
                     /gene="sarR"
                     /locus_tag="NBH32_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarR"
                     /protein_id="HCD1515927.1"
                     /translation="MSKINDINDLVNATFQVKKFFRDTKKKFNLNYEEIYILNHILRS
                     ESNEISSKEIAKCSEFKPYYLTKALQKLKDLKLLSKKRSLQDERTVIVYVTDTQKANI
                     QKLISELEEYIKN"
     gene            complement(639..1475)
                     /locus_tag="NBH32_002357"
     CDS             complement(639..1475)
                     /locus_tag="NBH32_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HCD1515928.1"
                     /translation="MDEQQWTGQLDLTVFFDGNRSVSRDIFFEKALKVIRPVYLNQST
                     IPTFYIVNVGGGYLDGDRYRMNVNVEDNAKVTLTSQGATKIYKTPSNHVEQYQTFNLK
                     DNAYLEYVADPIIAYENAKFYQHNTFNLNNSSSLFYTDILTPGYSKTGEAFKYQYMHL
                     INEIYIEDELVTYDNLLLNPNKQSINEIGYMEHYSHYGSAYFIHEDVNQKLIDSVYET
                     ISSYSNTFDCRVAISQLPTHGFAVRIFAYRTQIIEKILGTIQSYIAENIYDRKLDFLR
                     KY"
     gene            complement(1475..2089)
                     /gene="ureG"
                     /locus_tag="NBH32_002358"
     CDS             complement(1475..2089)
                     /gene="ureG"
                     /locus_tag="NBH32_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HCD1515929.1"
                     /translation="MANPIKIGIGGPVGAGKTQLIEKVVKRLSKEMSIGVITNDIYTK
                     EDEKILVNSGVLPESRIIGVETGGCPHTAIREDASMNFAAIDELLERHDDIELIFIES
                     GGDNLAATFSPELVDFSIYIIDVAQGEKIPRKGGQGMIKSDFFVINKTDLAPYVGASL
                     EQMAEDTKVFRGKRPFTFTNLKTDEGLDEVIDWIERDTLLKGLS"
     gene            complement(2102..2791)
                     /locus_tag="NBH32_002359"
     CDS             complement(2102..2791)
                     /locus_tag="NBH32_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreF"
                     /protein_id="HCD1515930.1"
                     /translation="MIDHTHLRLFQFCDSQFPTGAFSHSFGLETYIQRNIIHDDHTFI
                     AWLKMFLQEQLTYSDGLAMRLVYDALENDDTQKVLHIDKLMFVQNLPKETRVGAKQMG
                     TRMVKLALELYNSPWIAWYHQQMQDKKAKLNPAICFTMLGHYLGVDIETIIDYYLYQN
                     VSSLTQNAVRAIPLGQTAGQKIVTHMIPYIEETRKQIFELKEADFGMTAPGLELNQMA
                     HENVNVRIFIS"
     gene            complement(2784..3236)
                     /gene="ureE"
                     /locus_tag="NBH32_002360"
     CDS             complement(2784..3236)
                     /gene="ureE"
                     /locus_tag="NBH32_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HCD1515931.1"
                     /translation="MIVEEIQGNIANLSNSEKQKHVEKVYLENSDLVKRIQRVVTDHG
                     TEIGIRLKQPIDLQYGDILYADDHNMIIVDVNSEDLLVIQPRTLQEMGDIAHQLGNRH
                     LPAQFTETEMLVQYDYLVEDLLKSLGIPYVREDRKVNKAFRHIGHSHD"
     gene            complement(3249..4963)
                     /gene="ureC"
                     /locus_tag="NBH32_002361"
                     /pseudo
     CDS             complement(3249..4963)
                     /gene="ureC"
                     /locus_tag="NBH32_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002447295.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
     gene            complement(4960..5370)
                     /locus_tag="NBH32_002362"
     CDS             complement(4960..5370)
                     /locus_tag="NBH32_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit beta"
                     /protein_id="HCD1515932.1"
                     /translation="MIPGEIITKSTEIEINNHHPETVIEVENTGDRPIQVGSHFHFYE
                     ANAALDFEREMAYGKHLDIPAGAAVRFEPGDKKEVQLVEYAGKRKIFGFRGMVNGPID
                     ESRVYRPTDENDEYAGVFGDNGAENVNKKGGKRS"
     gene            complement(5384..5686)
                     /locus_tag="NBH32_002363"
     CDS             complement(5384..5686)
                     /locus_tag="NBH32_002363"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001832402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HCD1515933.1"
                     /translation="MHFTQREQDKLMIVVAAEVARRRKARGLKLNHPEALALISDELL
                     EGARDGKTVAELMSYGRQILNKEDVMDGVEHMITDIEIEATFPDGTKLITVHHPIV"
BASE COUNT         1840 a         1064 c          910 g         1994 t
ORIGIN      
        1 gtttagaatg aactttataa catagttgga tagagtttcg atttaataaa ttacatgtga
       61 accttgctac aacaagatgt gcatcagagg agtggtttaa taatgagtaa aattaatgac
      121 attaatgatt tagtcaacgc aacatttcaa gttaagaagt ttttcagaga tacaaaaaag
      181 aagttcaatt tgaactatga agaaatttat attttaaatc atattttaag aagtgagtct
      241 aacgaaatct catctaaaga gattgctaag tgctcagagt tcaaacctta ctatttaact
      301 aaagctttac aaaagctaaa agatttaaaa ttgttatcaa agaaaagaag tttacaagac
      361 gaaagaacag ttattgttta tgttacagat acacaaaaag caaatattca aaaactgatt
      421 tcagaattag aagaatacat taaaaattaa atcaaggtta attgcgttta ataacattga
      481 acgataacaa tttattaaaa cgaagttatt tattcagcat tgggacataa aattaactta
      541 aaatttaaat attgaagatg ctttaattaa agttaaagac tagccatacc ttatttcagc
      601 ttattaagct tgaaacaagg tacactagtc tttttatttt aatattttct tagaaaatca
      661 agtttacgat cataaatatt ttctgcgata tagctttgga tggttccaag tattttctct
      721 ataatttgtg tgcgataagc aaaaattcta actgcaaaac catgtgtagg caattgagaa
      781 atagcaacac gacaatcgaa tgtattgcta taagaactaa tggtttcata aactgaatcg
      841 attagttttt ggttaacatc ttcatgtatg aagtaagcag agccataatg agagtaatgt
      901 tccatataac ctatttcatt gatcgattgt ttattaggat tcaataataa attatcatat
      961 gtgactaact catcttcaat ataaatttca tttattaaat gcatatattg atacttgaag
     1021 gcttcgccag tttttgaata accaggagtt aaaatatcag tataaaataa tgaactagaa
     1081 ttattgagat tgaacgtatt gtgttgataa aatttagcat tttcatatgc gatgattgga
     1141 tcagcgacat attctaaata tgcgttatct ttcaaattaa aagtttgata ctgctcaaca
     1201 tgattggaag gtgtcttgta tatttttgtt gcaccttgag atgtcaatgt cactttagcg
     1261 ttatcttcga cattcacatt catacggtaa cgatctccat ctaaatagcc accacctaca
     1321 tttactatat aaaatgtagg aatggtagat tgatttagat aaactggacg tatcacttta
     1381 agtgcttttt caaagaaaat atctcttgat actgatcgat tgccatcgaa aaacactgtt
     1441 aaatcaagtt gcccagtcca ttgttgttca tccattatga taatcctttg agtaaagtgt
     1501 cgcgttcaat ccaatcgata acttcatcca aaccttcgtc agtttttaag ttagtaaaag
     1561 taaatggacg tttaccacga aatactttag tatcttcagc catttgttct aatgatgcac
     1621 ctacataggg agctaaatca gttttgttaa ttacaaagaa atctgactta atcatacctt
     1681 gaccaccttt acgtggaatc ttttcacctt gagcaacatc gataatataa attgaaaagt
     1741 caacaagttc tggactaaat gttgctgcta agttatcgcc accagattct atgaaaatta
     1801 gttctatatc gtcatgacgt tctaataatt cgtctattgc tgcaaagttc atagatgcat
     1861 cttcacgaat cgcagtatga ggacatccac cagtttcaac accaatgata cgactttcag
     1921 gtagaactcc tgaatttact aatatctttt cgtcttcttt tgtatatata tcatttgtaa
     1981 taacgccgat actcatttct tttgaaagac gttttacaac tttttcaatt aattgtgttt
     2041 tacctgcacc tacaggacca ccaataccaa ttttaatcgg gtttgccaca attataacct
     2101 cctatgatat gaaaattcta acattgacgt tctcatgcgc catttgattt agttctaaac
     2161 caggcgctgt catgccaaaa tctgcttctt ttaattcgaa aatctgcttt cttgtttctt
     2221 ctatataagg aatcatgtga gtaactatct tttgaccagc agtttgtcca agtggaatag
     2281 cacgaacagc attttgagtt aaacttgaaa cattttgata taaatagtaa tcaataatcg
     2341 tttcaatatc tacacctaaa taatggccta gcatagtaaa acaaatagct ggatttaact
     2401 ttgctttctt atcttgcatt tgttgatgat accaagcaat ccatgggcta ttataaagtt
     2461 ctaaagccaa tttaaccatg cgagtcccca tttgttttgc accaacacgt gtttctttag
     2521 gtaagttttg aacaaacatc agcttatcta tgtgtaatac tttttgtgta tcatcatttt
     2581 ccaatgcatc ataaactaaa cgcatggcta aaccatcaga ataggtaagt tgctcttgta
     2641 aaaacatttt taaccaagca ataaaagtat gatcatcatg aattatattt cgttgaatat
     2701 atgtttcaag accaaatgaa tgactgaaag cacctgttgg aaactgtgaa tcacagaact
     2761 gaaataatct taagtgtgta tgatcaatca tgagaatggc ctatatgtct gaaagcctta
     2821 ttaactttac ggtcttctcg aacatatggg atgcctaaac tttttaataa atcttcaact
     2881 aaataatcat attgtactag catttcagtc tctgtaaatt gtgctggcaa atgacgattt
     2941 cctaattgat gggctatatc tcccatttct tgcaatgttc ttggttgaat cactaaaaga
     3001 tcttctgaat taacatccac aataatcata ttatggtcat ctgcgtataa aatatctcca
     3061 tattgtaagt caataggttg ttttaaacga atgcctattt cagtgccatg gtctgtaacg
     3121 actctttgaa tacgtttaac gagatctgaa ttttcaaggt atactttttc gacgtgcttt
     3181 tgtttttctg aatttgacaa attggcaata ttgccttgga tttcttcaac aatcattcta
     3241 tgttcctcct agaataagaa gtatctttga gttaatggta actcagttgc tgcattactt
     3301 gtaatttttt ctccatctac atatacttca tatgtttgtg gatcaacgtc taatttaggt
     3361 gttgcgctgt tatttttcat atctgcttta gataattgtc taatattttt aactggacgc
     3421 accatgcgtt ttaaatttaa tgcacgattg ataccatttt cataagcagt tttagacacg
     3481 aatgtcattg acgtacttgt aaggtttccg ccgtattgac catacatttt acggtacttc
     3541 attggttcag atgtagggat agaaccattt gcatcgccat ttacggcaga gttaattaat
     3601 ccgcccttta caactaattc aggtttaaca ccaaagaaaa tgggatccca taagacaatg
     3661 tcagctagtt tgcccggctc gatagatcct acatattcag aaataccatg tgtaattgct
     3721 gggttaattg tatatttagc gatataacgt ttgatgcgat tattatcatt atgttcaaaa
     3781 tcaccatcta aaggaccacg ttgttctttc atgcgatgtg ctacttgcca tgttcgtgta
     3841 attacttcac ctacacggcc cattgcctgt gaatcggaac taatcatact gaatacaccc
     3901 atatcttgca ggacatcttc tgctgcaatc gtttctttac gaatacgtga atctgcgaat
     3961 gcgatatctt caggaatagc cgcatttaaa tggtgagtaa tcattaccat atctaaatgt
     4021 tcatctacag tattatgtgt atatggcaat gttggatttg tagatgaagg taaaatattt
     4081 gaaaatgcag cggatttaat taaatcaggc gcatgaccgc caccagcacc ttcagtatgg
     4141 tacatatgaa gtacacggtc tttaacagca gccattgtgt cttccataaa tcctgcttca
     4201 tttaaagtat ctgcatgtaa tgcaatttga atatcaaatt catcagcaac atctaatgca
     4261 tgactcaaag cagatggtgt tgcaccccag tcttcatgta cttttaatcc aattgctccg
     4321 gcattgattt gttcaatgag tgcagttgga tttgttgctt gtcctttacc tgtaaaaccg
     4381 acattaatcg gtaaaccttc ggcagcttct aacattctat gaatatgcca tggacctgga
     4441 gttacagttg ttgctttaga accttctgaa gcaccagtac caccaccaat atgagtcgta
     4501 ataccacttt ctaatgcgac ctctgcttgt tcaggattaa taaaatgaac atgagtatca
     4561 ataccaccag cagtgacgat tttaccttca gcggcaatga tatctgttgt tgatcctata
     4621 ataatgtcga cattatccat tatatccggg ttgccggcat ttccaatggc gaaaatataa
     4681 ccatttttaa tacctatatc ggctttaacc actttatcgt aatcgataat aacggcatta
     4741 gaaataacaa ggtctgcaac gttcacgtca tcacgtgtta cacgaggatt ttgcgccata
     4801 ccgtctctaa tagattttcc accgccaaag gtagcttctt caccataaac tgcatagtct
     4861 ttttctattt gagcaaatag gttcgtatca cctaacgaat agaatctcca acagtaggac
     4921 cgtataagct cgtatattga ttttgcgtca ttttaaagct catgatcttt ttcctccttt
     4981 tttattcacg ttttcagcac cgttatctcc gaatacacct gcatattcat cattttcatc
     5041 agtcgggcga tagacacgtg actcatcgat aggaccattg accataccac gaaaaccaaa
     5101 aattttacgt ttgccagcat attcaactaa ttgaacttct tttttatccc caggttcaaa
     5161 tcgaacagct gctccagctg gaatatctaa atgttttcca tatgccattt cacgttcgaa
     5221 atctaatgct gcatttgctt cataaaaatg aaaatgtgag cccacttgaa ttggtcgatc
     5281 tcctgtattt tcaacttcga taactgtttc aggatgatgg ttattaattt caatctctgt
     5341 actttttgta ataatttctc ctggtatcat ttgactgcct cctttaaaca atagggtgat
     5401 gtactgtgat taacttagta ccatcgggga acgtagcctc gatttcgata tctgtaatca
     5461 tgtgttcgac accatccatg acatcttctt tgtttagaat ttgtctacca taactcatta
     5521 actctgcaac ggtcttacca tcgcgtgcac cttctaataa ttcatcgctg attaaagcta
     5581 atgcctcagg atgatttagt ttcaaaccac gtgctttacg acgacgtgca acttccgctg
     5641 ccactacaat cattaatttg tcttgctctc gttgtgtaaa atgcaaatta aaacccccaa
     5701 tttcatatta gatacaattt acaaaattta tattaatcct aattgttgtg ataaataagt
     5761 aatatacaaa gtttaatgcg taacagtaaa aaattaattt actatttc
//
LOCUS       DAHHOJ010000132         5784 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.132, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000132 DAHHOJ010000000
VERSION     DAHHOJ010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5784
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.132"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            94..1170
                     /locus_tag="NBH32_002364"
     CDS             94..1170
                     /locus_tag="NBH32_002364"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HCD1515934.1"
                     /translation="MSQAVKVERRETLKQKPNTSQLGFGKYFTDYMLSYDYDAEKGWH
                     DLKIVPYGPIEISPAAQGVHYGQSVFEGLKAYKKDGEVALFRPEENFKRLNNSLARLE
                     MPQVDEAELLEGLKQLVDIERDWIPEGEGQSLYIRPFVFATEGALGVGASHQYKLLII
                     LSPSGAYYGGETLKPTKIYVEDEYVRAVRGGVGFAKVAGNYAASLLAQTNANKLGYDQ
                     VLWLDGVEQKYIEEVGSMNIFFVENGKVITPELNGSILPGITRKSIIELAKNLGYEVE
                     ERRVSIDELFESYDKGELTEVFGSGTAAVISPVGTLRYEDREIVINNNETGEITQKLY
                     DVYTGIQNGTLEDKNGWRVVVPKY"
     gene            1420..2103
                     /locus_tag="NBH32_002365"
     CDS             1420..2103
                     /locus_tag="NBH32_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HCD1515935.1"
                     /translation="MEWILFDKDGTLIEFDRSWEKIGVRFVQSLLETFPVHNKEAALR
                     QLGVIKESIDPKSVMGSGSLQQIIQAFNDVTGQDTTDWSKSTSQKLVDERIPEINWVE
                     GVKEALIDLKAKGYQLGIVTSDTKKGVEQFLAHTNAISLFDLIISTEADAYEKPNPKV
                     LSPLFEQYNVDPQKVAIVGDTANDMKTASNANLGMAIGVLTGIATKEELHEADIILNS
                     AADILEALN"
     gene            complement(2219..2881)
                     /locus_tag="NBH32_002366"
     CDS             complement(2219..2881)
                     /locus_tag="NBH32_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1515936.1"
                     /translation="MNNYGIPQNAIITIAGTVGVGKSTLTQALADKLNFKTSFENVEH
                     NPYLDKFYSDFERWSFHLQIYFLAERFKEQKRMFEYGGGFVQDRSIYEDVDIFAKMHE
                     EEGTMSKEDFKTYSDLFNAMVMTPYFPKPDVMIYLECNYDEVIDRIIERGREMEINTD
                     PEYWKKLFKRYDDWINSFNACPVVRININEYDIHKDPESLNPMIDKIARIIQTYRQVD
                     TR"
     gene            complement(2874..3491)
                     /locus_tag="NBH32_002367"
     CDS             complement(2874..3491)
                     /locus_tag="NBH32_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HCD1515937.1"
                     /translation="MNKPFIAIEGPIGVGKSSLAHKLSQTLDFYEEKEIITENPFLSD
                     FYEDISKWSFQTEMFFLCNRYKQFQDVTQLNQGVVSDYHIHKNKIFAKNTLSSVEFQK
                     FSKIYDILTEDMIMPNMIIFLDADLDVLKSRIAKRNRSFEHQIEDEYLLKLKKDYREY
                     YESLQSNGSNVVLIDTTSIDFLKNEQDYEDILHIILPMIGDITNE"
     gene            3558..4028
                     /locus_tag="NBH32_002368"
     CDS             3558..4028
                     /locus_tag="NBH32_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HCD1515938.1"
                     /translation="MTNDIYFMTLAIEEAKKAAQLGEVPIGAIITKDDEVIARAHNLR
                     ETLQQPTAHAEHIAIERAAKVLGSWRLEGCTLYVTLEPCVMCAGTIVMSRIPRVVYGA
                     DDPKGGCSGSLMNLLQQSNFNHRAIVDKGVLKEACSTLLTTFFKNLRANKKSTN"
     gene            4175..5044
                     /locus_tag="NBH32_002369"
     CDS             4175..5044
                     /locus_tag="NBH32_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1515939.1"
                     /translation="MIKLIATDMDGTLLNAAHEISQPNIDAIKYAQEQGITVVIATGR
                     AFYEAQAPVADTDLTVPYICLNGAEVRDETFNVMSTSHLNKSLVHKITNVLKDAGIYY
                     QVYTSRAIYTEDPQRDLDIYIDIAERAGQHANVERIKNGIQRRIDNGTLKVVDNYDAI
                     ENIPGELIMKILAFDGNLEKIDKASKILAESPNLAISSSSRGNIEITHSDAQKGIALE
                     TIAERLGIEMKDVMAIGDNLNDLSMLEKVGYPVAMENGAEEVKKIAKYVTDTNENSGV
                     GKAIMKLLREQQV"
     gene            5065..5631
                     /locus_tag="NBH32_002370"
     CDS             5065..5631
                     /locus_tag="NBH32_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCD1515940.1"
                     /translation="MKGLIIIGSAQVNSHTSALARYLTEHFKTHDIEAEIFDLAEKPL
                     NQLDFSGTTPSIDEIKQNMKDLKEKAMAADFLILGTPNYHGSYSGILKNALDHLNMDY
                     FKMKPVGLIGNSGGIVSSEPLSHLRVIVRSLLGIAVPTQIATHDSDFAKNEDGSYYLN
                     DSEFQLRARLFVDQIVSFVNNSPYEHLK"
BASE COUNT         2102 a          796 c         1041 g         1845 t
ORIGIN      
        1 gaatttttag aaaatttgtt gaacagcaaa atatggattg ttataattta agttaaacaa
       61 attcttatac aatattatta ggaggcaatc accatgtcac aagcagttaa agttgaacga
      121 cgagaaacat taaaacaaaa accaaataca tctcaactag gttttggtaa atattttact
      181 gattatatgt tgagttatga ttatgatgca gaaaaaggat ggcatgattt gaagatagta
      241 ccttatggtc ctattgaaat ttcacctgct gcacaaggtg ttcattatgg tcaatcggta
      301 ttcgaaggat taaaagcata taaaaaagat ggggaagttg cacttttccg tcctgaagaa
      361 aattttaagc gtcttaataa ctcgttagca cgattagaaa tgcctcaagt agacgaagca
      421 gaattgttag aggggctaaa acaattagtt gatatagaaa gagattggat tcctgaaggg
      481 gaaggtcaat cattatatat tcgtccattt gtttttgcaa cagaaggggc acttggcgtt
      541 ggtgcatcac atcagtataa attattaatt attttatctc cttcaggtgc atattatggt
      601 ggtgagactt taaaaccaac taaaatctat gtagaagatg aatatgtacg tgctgttcgt
      661 ggcggtgtag gctttgcaaa agttgcaggt aactatgcgg caagtttatt agcacaaact
      721 aatgcaaata aattaggtta tgaccaagta ttatggcttg atggtgttga acagaaatat
      781 atcgaagaag ttggtagcat gaacattttc ttcgttgaaa atggcaaagt aattacacca
      841 gagttgaatg gcagtatttt acctggtatt acacgtaaat ctattatcga attagctaaa
      901 aacttaggat atgaagtcga agagcgccgc gtttcaatcg atgaattatt cgaatcatat
      961 gataaaggtg agttaacaga agtatttggt agtggtactg cagcagttat ttcacctgtg
     1021 ggtacattga gatacgaaga tcgtgaaatc gttattaata ataatgagac tggtgaaatt
     1081 actcaaaaat tatacgacgt ctatactggt attcaaaatg gtactttaga agataaaaat
     1141 ggttggagag tcgttgtacc aaaatattaa taaaaattga atataatcat gaaaacaata
     1201 tgtaaaagtt aatcatgact aaaatccctt tcaatgaagc gaagcatggt aataaattaa
     1261 gtttacaatg tttattgtta agtattgaag gggatttcac ttattattat atttaattca
     1321 atatttaaat agaacaatac tacgagttca ttttcagggg ataacgaata tcatagaatc
     1381 agcataatac gaaataaata aataggagta ttgatatcaa tggaatggat attatttgat
     1441 aaagatggta cgttaattga atttgataga agttgggaaa aaataggggt acgatttgta
     1501 caatcattgc ttgagacttt cccagtacat aataaagaag ctgctttaag acaactcggt
     1561 gtcattaaag aatctattga tccaaaatca gtgatgggtt caggatcttt acaacaaatt
     1621 atccaggcat ttaatgatgt gacgggacaa gatacaaccg actggtccaa gtcaacaagt
     1681 caaaagctgg tagatgaacg tattcctgaa attaattggg tagaaggtgt taaagaagca
     1741 cttatcgatt tgaaagcaaa aggctatcaa cttggtattg ttacgagtga tactaaaaaa
     1801 ggtgtagaac aatttttagc acataccaat gctatctcgt tgttcgattt gatcatttct
     1861 accgaagcgg atgcctatga gaagccaaat cctaaagtat tatcgccttt atttgagcaa
     1921 tataatgtag atcctcagaa agtagctata gtaggagaca ctgctaatga tatgaagaca
     1981 gcaagtaatg caaatttagg tatggcaata ggtgtattaa caggtattgc aacaaaagaa
     2041 gaattacatg aagctgatat tattttaaat agtgcggcag atattttaga agctttaaat
     2101 taaaaagaaa gacatagtgt atatgtgaat taatgtattc gatagaattg ataatgtaat
     2161 cgatgatatc tatggaatat acattgaata taaacatata cgctatgtct ttagtctttt
     2221 atcgtgtatc tacttgtcga tatgtttgaa taattcgagc aattttgtct atcataggat
     2281 ttaaagattc ggggtcctta tggatatcat attcattaat attgatacgt acaactggac
     2341 atgcattaaa gctattaatc caatcgtcat agcgtttaaa tagctttttc cagtattcag
     2401 ggtctgtatt aatttccatt tcgcgaccac gttcaataat acgatcaatg acctcatcat
     2461 agttacattc taaataaatc attacatcag gtttaggaaa ataaggtgtc atgaccatgg
     2521 cattaaataa gtctgaatat gttttgaaat cttctttact cattgtgcct tcttcttcat
     2581 gcatttttgc aaaaatatca acatcttcat aaattgatcg atcttggaca aagccaccac
     2641 catattcaaa catacgcttt tgttctttaa aacgttcagc taagaagtaa atttgcaaat
     2701 ggaaactcca tcgttcaaaa tcgctgtaaa atttatctaa atatggatta tgttcgacat
     2761 tttcaaaaga cgttttaaag tttaatttat ctgcaagtgc ttgcgttagt gttgattttc
     2821 caacaccaac tgtacctgca atggttataa tggcattttg tggaataccg taattattca
     2881 ttggtaatat ctcctatcat aggtaatata atatgtaata tatcttcgta atcttgttca
     2941 tttttaagaa aatcaataga agttgtatcg attaaaacta catttgaacc attactttgt
     3001 aaggactcat aatactcacg ataatctttt tttaacttta acagatattc atcttctatt
     3061 tgatgctcaa aactacggtt acgtttagca attctagatt ttaacacatc aaggtctgca
     3121 tctaaaaaga taatcatatt cggcataatc atatcttcag ttaaaatatc ataaatttta
     3181 ctgaatttct gaaattcaac agaactcaaa gtatttttag caaatatctt atttttatgt
     3241 atatgataat cactaactac accttgattt agttgtgtta catcttgaaa ttgcttatat
     3301 ctattgcata aaaagaacat ttcagtttga aaactccatt tagagatatc ttcataaaag
     3361 tctgataaaa atggattttc tgtgatgatt tctttttctt cataaaaatc taaagtttga
     3421 cttaatttgt gtgcaagtga agatttacct acgccaatag gaccttcaat tgctataaaa
     3481 ggtttgttca tattcacatc tccaaatgtt aaaatagaca ttgagtattg taccataagt
     3541 gaggtatctg gacataaatg acaaatgata tatattttat gacattagcg attgaagaag
     3601 ctaaaaaagc agctcaacta ggcgaagtac ctataggtgc tatcatcact aaagatgatg
     3661 aagttatcgc tagagcacat aatttaagag aaacactaca acaaccaacg gcgcatgctg
     3721 aacatattgc aattgaacgt gcagccaaag tgttaggtag ttggcgttta gaaggttgca
     3781 cattatatgt aaccttagaa ccatgtgtca tgtgcgcagg aacaattgta atgagtcgca
     3841 ttccaagagt cgtctatggc gcagatgatc ctaaaggtgg ttgtagtggc agtttaatga
     3901 atttattgca acaatctaat tttaatcatc gtgcaattgt tgataaaggt gtacttaaag
     3961 aagcatgtag cacattatta acaacatttt ttaaaaactt aagagccaat aagaaatcca
     4021 ccaattagac gatagtacat actaattaaa atttgttaga attacattat atgataaatt
     4081 aatgacaata taaatgttaa tagaaaagta tgtgcgctga gtatatattc ttatttaaga
     4141 acaagatttt aaatttacga aacgaggtaa cataatgata aaactaatag ccactgatat
     4201 ggatggtacg ctacttaatg cagcacatga aatttctcaa cctaatattg atgcgattaa
     4261 atacgctcaa gaacaaggga taacggttgt tatcgcgaca ggtcgagcat tttatgaagc
     4321 acaagcacca gttgctgaca cagatttaac agtaccatat atttgtttga atggtgctga
     4381 agtacgtgat gaaactttca atgtaatgag cacttcacac cttaataaat cgttagtaca
     4441 caaaattaca aatgttttaa aagatgcagg tatttattat caagtataca cgagtcgtgc
     4501 gatttatact gaagatccac aaagagattt agacatttac atagatattg ctgagcgtgc
     4561 aggtcaacat gcaaacgttg agcgtattaa aaatggtatt caaagacgca tagataatgg
     4621 tacgttgaaa gttgttgata attatgatgc tattgaaaac atacctggtg aattaattat
     4681 gaaaatatta gcatttgatg gaaatttaga aaaaattgac aaagctagta aaattttagc
     4741 tgaatctccg aatttagcta tatcatcatc ttcgagagga aatatagaaa taacgcattc
     4801 agatgcacaa aaaggtattg cgctagaaac aattgccgaa agattaggga ttgaaatgaa
     4861 agatgtcatg gcaataggtg acaatttaaa tgacttatca atgttagaga aagttggcta
     4921 tccagttgcg atggaaaatg gtgcagaaga agttaaaaaa atagcgaaat atgtcacaga
     4981 tacgaatgaa aatagtggtg ttggaaaagc tattatgaaa ttattacgtg aacaacaagt
     5041 ttaataaaaa aagaggggtc aaatatgaaa ggattaatta ttattggcag tgcacaagtg
     5101 aattcacata caagtgcact agcaagatac ttaactgagc attttaaaac acatgatatt
     5161 gaagcggaaa tattcgattt agcagaaaaa ccgttaaatc aattagattt ttcaggaaca
     5221 acaccgtcta ttgatgaaat caaacaaaat atgaaagatt taaaagagaa agcaatggcg
     5281 gcggactttt taatattagg aacgccaaac tatcatggtt catattctgg aatattgaaa
     5341 aatgcattag atcatctaaa tatggattat tttaaaatga aacctgtagg cttaatagga
     5401 aatagtggtg gtattgttag ttcagagcca ttgtcacatt taagagtaat cgtcagaagt
     5461 ttactaggca ttgctgtacc aactcaaata gcaacacatg attctgattt tgctaaaaat
     5521 gaagatggtt catattactt aaatgatagt gaattccaat tacgagcaag attatttgtc
     5581 gatcaaattg tatcttttgt gaataatagt ccatatgaac atttaaaata atattaaaaa
     5641 atatgtaaat atacattaaa aataggtatg tagcttaagg tatttcattg agaatgcttt
     5701 taagtcacat gcctattttt tgttctaatg caccaatagt aagcctttga gattactgtt
     5761 gtgagcaatg ttattaatta aaat
//
LOCUS       DAHHOJ010000133         5659 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.133, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000133 DAHHOJ010000000
VERSION     DAHHOJ010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5659
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.133"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            120..1520
                     /locus_tag="NBH32_002371"
     CDS             120..1520
                     /locus_tag="NBH32_002371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCD1515941.1"
                     /translation="MDFNKENINMVDAKKAKKTVVATGIGNAMEWFDFGVYAYTTAYI
                     GANFFSPVENADIRQMLTFAALAIAFLLRPIGGVVFGIIGDKYGRKVVLTSTIILMAF
                     STLTIGLLPSYDQIGLWAPILLLLARVLQGFSTGGEYAGAMTYVAESSPDKLRNSLGS
                     GLEIGTLSGYIAASIMIAVLTFFLTDEQMASFGWRIPFLLGLFLGLFGLYLRRKLEES
                     PVFENDVATQPERDNINFLQIIRFYYKDIFVCFVAVVFFNVTNYMVTAYLPTYLEQVI
                     KLDATTTSVLITCVMAIMIPLALMFGKLADKIGEKKVFLIGTGGLTLFSIIAFMLLHS
                     QSFVVIVIGIFILGFFLSTYEATMPGSLPTMFYSHIRYRTLSVTFNISVSIFGGTTPL
                     VATWLVTKTGDPLAPAYYLTAISVIGFLVITFLHLSTAGKSLKGSYPNVDNEQDRAYY
                     AEHPKEALWWVKERKN"
     gene            2064..3440
                     /locus_tag="NBH32_002372"
     CDS             2064..3440
                     /locus_tag="NBH32_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HCD1515942.1"
                     /translation="MNVILEQLKTHTQNKPNDIALHIDDETITYSQLNARITSAVESL
                     QKYSLNPVVAINMKSPVQSIICYLALHRLHKVPMMMEGKWQSTIHRQLIEKYGIKDVI
                     GDTGLMQSIDSPMFIDSTQLQHYPNLLHIGFTSGTTGLPKAYYRDEDSWLASFEVNEM
                     LMLKNENAIAAPGPLSHSLTLYALLFALSSGRTFIGQTTFHPEKLLNQCRKISSYKVA
                     MFLVPTMIKSLLLVYNNEHTIQSFFSSGDKLHSSIFKKIKNQANDINLIEFFGTSETS
                     FISYNLNQQAPVESVGVLFPNVELKTTDHDHNGIGTICIKSNMMFSGYVSEQCINNDE
                     WFVTNDNGYVKEQYLYLTGRQQDMLIIGGQNIYPAHVERLLTQSSSIDEAIIIGIPNE
                     RFGQIGVLLYSSDVTLTHKNVKQFLKKKVKRYEIPSMIHHVEKMYYTASGKIAREKMI
                     SMYLRGEL"
     gene            3442..4581
                     /locus_tag="NBH32_002373"
     CDS             3442..4581
                     /locus_tag="NBH32_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HCD1515943.1"
                     /translation="MNQAVIVAAKRTAFGKYGGTLKHLEPEQLLKPLFQHFKEKYPEV
                     ISKIDDVVLGNVVGNGGNIARKALLEAGLKDSIPGVTIDRQCGSGLESVQYACRMIQA
                     GAGKVYIAGGVESTSRAPWKIKRPHSVYETALPEFYERASFAPEMSDPSMIQGAENVA
                     KMYDVSRELQDEFAYRSHQLTAENVKNGNISQEILPITVKGEIFNTDESLKSHIPKDN
                     FGRFKPVIKGGTVTAANSCMKNDGAVLLLIMEKDMAYELGFEHGLLFKDGVTVGVDSN
                     FPGIGPVPAISNLLKRNQLTIENIEVIEINEAFSAQVVACQQALNISNTQLNIWGGAL
                     ASGHPYGASGAQLVTRLFYMFDKETMIASMGIGGGLGNAALFTRF"
     gene            4556..4921
                     /locus_tag="NBH32_002374"
     CDS             4556..4921
                     /locus_tag="NBH32_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraC"
                     /protein_id="HCD1515944.1"
                     /translation="MQHYSLDSNQRLNVSFSKDSVAAYYQCFNQPYRKEVPPLMCASL
                     WSKFDLFKKYANSELILTKSAINQTQKIEVDTIYVGRLEDIECRQIRNITRYTMALTL
                     TKNDQHVITVTQTFIKAMK"
     gene            4924..5193
                     /locus_tag="NBH32_002375"
     CDS             4924..5193
                     /locus_tag="NBH32_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515945.1"
                     /translation="MKFNEIWINEYLALVNDDNPIHNEIVPGQLVSQMMLMAMSLETN
                     QCQINYVKPILINENIEFIEQHEQEIIAINDDGEIKIKISLSTKK"
     gene            complement(5394..5561)
                     /locus_tag="NBH32_002376"
     CDS             complement(5394..5561)
                     /locus_tag="NBH32_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein VraX"
                     /protein_id="HCD1515946.1"
                     /translation="MIIYRQYHHEGAPVYEIITKTFQHVSIKCDDSFSDTEIFKLLSL
                     LQDDIDHMKVS"
BASE COUNT         1958 a          822 c         1052 g         1827 t
ORIGIN      
        1 gtgaaatttt tgaacaaaca tacttttgta tttatataaa agtttaaatt cttataaatt
       61 tgacaaaact aattaactcc gtataattat gaaacataca agagggagtg tatgaattca
      121 tggattttaa taaagagaat attaacatgg tggatgcaaa gaaagctaaa aaaaccgttg
      181 ttgcaaccgg tatcggtaat gcaatggaat ggttcgattt tggtgtctat gcatatacaa
      241 ctgcgtacat tggagcgaac ttcttctctc cagtagagaa tgcagacatt cgacaaatgt
      301 tgactttcgc agcattagcc attgcgtttt tattaagacc aattggtggt gtcgtatttg
      361 gtattattgg tgacaaatat ggacgtaaag ttgtattaac atctacaatt attttaatgg
      421 cattttcaac attaaccatt ggattattgc caagctatga tcaaattgga ctttgggcac
      481 caatactatt attgcttgca agagtactgc aaggattttc aacaggtgga gagtatgcgg
      541 gggcaatgac atatgttgcc gaatcatctc cagataagct tcgtaactca ttaggtagtg
      601 gactagaaat tgggacatta tcaggttaca tagctgcttc aattatgatt gctgtattaa
      661 cattcttttt aacagatgaa caaatggcat catttggttg gagaatccca ttcttacttg
      721 gtttattcct aggattattc ggcttatatt tacgtcgtaa gcttgaagaa tcaccagttt
      781 tcgaaaatga tgttgcaaca caaccagaac gagacaacat taacttttta caaatcatca
      841 gattttatta caaagatata tttgtgtgtt ttgtagctgt tgtattcttc aatgttacga
      901 actatatggt aactgcatat ttaccaacct atttagaaca agttattaaa ttagatgcaa
      961 cgacaacaag tgtattaatt acttgtgtca tggcaataat gattccatta gcattaatgt
     1021 ttggtaagtt agcggataaa ataggtgaaa agaaagtatt tctaattggt actggtgggc
     1081 taacattatt tagtatcatc gcatttatgt tattacattc acaatcattt gttgtaatag
     1141 taatcggtat atttatatta ggatttttct tatcaactta cgaagcgaca atgccagggt
     1201 cgttaccaac gatgttttac agtcatataa gatatcgaac tttatcagta acatttaata
     1261 tctctgtttc gatatttggt ggtacgacgc cattagttgc aacatggtta gttacgaaaa
     1321 ctggagatcc attagcacct gcgtattatt taacagcaat cagtgttatt ggctttttag
     1381 ttattacatt cttacattta agtacagcag gaaaatctct aaaaggttcg tatccaaatg
     1441 tagataacga gcaagataga gcttattatg cagaacaccc aaaagaagca ttatggtggg
     1501 ttaaagaacg taagaattag agattttaat aaaaagtata aatcaatcgt atataagcac
     1561 tttaaagcta gtaggttctg ctaactttaa agtgcttttt aaattgagaa ctgtaattag
     1621 ccgtaataaa gtttttgtat atacataaac ccccactgca atgattatcg caatggggga
     1681 aagaggggac ttaaagcata tgtttagctt tgaatactta aaattctctt gctattgaaa
     1741 tgttaggatg taaatatgtc ttagagtatt ttgtccaacg caattaatat tgagactcta
     1801 accttcaata ttattataga gaacacaaac ttaaatagat tgggtgactt atttgtgtca
     1861 gttattgcga ttgcgataac ttcttttctc tatatacata tagtaacgtc ttatctaata
     1921 aaaaacatgg tactacagta tcaaatttat ctagggctta agtttgattt ttataatagg
     1981 caggtttacc tgataaaaat acttattcat tatataatgt taacaagatg tattttaaag
     2041 tttacattga gtgagggata ttgatgaacg taattttaga acagttgaaa acacatactc
     2101 aaaataaacc taatgacata gcattacata tcgatgatga aacaattaca tatagtcaac
     2161 taaatgcccg catcactagt gcagttgaat ctttgcagaa atattcactt aatcctgtcg
     2221 ttgctattaa tatgaaatca ccggtgcaaa gtattatttg ttatttagct ttgcatcgtt
     2281 tacataaagt gcctatgatg atggaaggta aatggcaaag tactatacat cgtcaattga
     2341 ttgaaaaata tggtattaaa gatgtaattg gagatacagg tctcatgcag agtatagatt
     2401 caccgatgtt tattgattca acgcaattac agcactatcc caatttatta catattggtt
     2461 ttacttcagg gacaactgga ctgccaaaag catattatcg tgatgaagat tcatggttgg
     2521 cttcttttga agttaatgaa atgttgatgt taaaaaatga aaatgcaata gcagcccctg
     2581 gaccactatc gcactcgtta acattatatg cgttattgtt tgctttaagt tccggtcgta
     2641 cttttatagg acagaccact tttcatcctg aaaagttact taatcaatgt cgtaaaatat
     2701 catcatacaa agttgctatg tttcttgttc caacgatgat taaatcatta ttgttagttt
     2761 acaacaatga acatacaatc caatcatttt ttagcagtgg agataagctg cattcttcta
     2821 tttttaaaaa gataaaaaat caagcaaatg acataaattt gattgaattt tttggtacat
     2881 cggaaaccag ttttatcagc tataacttga atcagcaagc accagttgaa tcagtaggtg
     2941 tgctatttcc aaatgtggaa ttgaaaacaa cggatcacga tcacaatggt ataggaacta
     3001 tttgtataaa aagtaatatg atgtttagtg gctatgtaag tgaacaatgt ataaataatg
     3061 atgaatggtt tgttactaat gataatggct atgtaaaaga gcagtattta tatttaacgg
     3121 gacgtcaaca ggatatgtta attattggtg gtcaaaatat atatccagca catgttgaac
     3181 gccttttaac gcaatcgtcg agcattgatg aagcaattat catcggtatt ccaaatgagc
     3241 gttttggtca aataggcgta ttgctttatt ctagtgatgt gacacttaca cataaaaatg
     3301 taaaacaatt tttaaaaaag aaagtgaaac gctatgaaat tccatcgatg attcatcatg
     3361 tagaaaagat gtattacact gcaagtggta aaattgctag agaaaaaatg atatcgatgt
     3421 atttgagagg tgaattataa tatgaatcaa gcagtcatag ttgcagctaa acgaactgca
     3481 tttgggaaat atggtggcac tttaaaacat ttagagccag aacaattgct taaaccttta
     3541 ttccaacatt ttaaagagaa gtatccagag gtaatatcta aaatagatga tgtagtttta
     3601 ggtaatgttg ttgggaatgg tggcaatatt gcaagaaaag cattgcttga agcggggctt
     3661 aaagattcaa tacctggcgt cacaatcgat cggcaatgtg ggtctggact tgaaagtgtt
     3721 caatatgcat gtcgcatgat ccaagccgga gctggcaagg tatatattgc aggtggtgtt
     3781 gaaagtacaa gtcgagcacc ttggaaaatc aaacgaccgc attctgtgta cgaaacagca
     3841 ttacctgagt tttatgagcg tgcatcattt gcacctgaaa tgagcgaccc atcaatgatt
     3901 caaggtgctg aaaatgtggc caagatgtat gatgtttcaa gagaattaca agatgaattt
     3961 gcttatcgaa gtcatcaatt gacagcggaa aatgtaaaga atggaaatat ttctcaggaa
     4021 atattaccta taaccgttaa aggagaaata ttcaacactg atgaaagtct aaaatcacat
     4081 attccgaaag ataactttgg ccgatttaag cccgtgatca aaggtgggac cgttaccgct
     4141 gcgaatagtt gtatgaaaaa tgatggtgca gttttattgc ttattatgga aaaagatatg
     4201 gcatacgaat taggtttcga gcatggttta ttatttaaag atggtgttac ggtaggtgtt
     4261 gattctaatt ttcctggcat tggtccagta ccagccattt ccaacttact aaaaagaaat
     4321 caattaacga tagaaaatat tgaagtcatt gaaattaacg aagcgttcag tgcacaggtg
     4381 gttgcctgcc aacaagcttt aaatatttca aatacgcaat taaatatatg gggtggtgca
     4441 ttagcatcag gtcatccata cggtgcaagc ggtgcccaat tagtgacgcg attattttat
     4501 atgtttgaca aagagactat gattgcatct atggggattg ggggaggtct aggaaatgca
     4561 gcattattca ctcgattcta accagcgatt aaatgtgtca ttttctaagg atagtgtggc
     4621 tgcatattat cagtgtttta accaacctta tagaaaagaa gtaccaccat taatgtgtgc
     4681 gtcattatgg tcaaaatttg atttatttaa aaaatatgca aatagcgaac tgattttaac
     4741 aaaatcagca attaatcaaa ctcaaaagat agaagtagac acaatatatg taggacgttt
     4801 agaagatatt gaatgccgac agattcgcaa tatcacacgt tatacaatgg ctttaacatt
     4861 aactaaaaat gatcaacatg tcataacggt tacacaaact tttattaagg cgatgaagta
     4921 gagatgaagt ttaatgagat atggataaat gaatatttgg cgctcgtaaa tgatgataat
     4981 ccaatacata atgagattgt gccaggacaa ttagtgagtc aaatgatgct gatggctatg
     5041 tcattagaga caaaccagtg tcaaattaac tacgttaaac ctattttaat aaatgaaaat
     5101 atcgaattca ttgaacaaca cgagcaagaa attatagcaa ttaatgacga tggagagatt
     5161 aaaataaaaa tttctttgag cacaaaaaaa taaccgatat tagctgcatg aacgcatatt
     5221 aattaggaga tgaaaggaca gctaatatca gttatgtatt gttattatta ttgggaacag
     5281 agatgaatat aggttacgat tctttctttg cacggggatg cattaatcta aaataataat
     5341 aacaactata tcaatgttta ataaattctg gattattgga acgattagtc aatttaacta
     5401 actttcatat gatctatatc gtcttgtaat aaagagagca atttgaatat ttcagtatca
     5461 ctaaatgaat cgtcacattt aattgaaaca tgctgaaacg ttttggttat aatttcataa
     5521 actggtgcgc cttcatggtg atactgtcga taaataatca taacctatat tacctccttt
     5581 gctactctat ggttatatta taaataacat ttttatgtgt gacatcaacc ttaagtatca
     5641 actttttatc agacataga
//
LOCUS       DAHHOJ010000134         5555 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.135, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000134 DAHHOJ010000000
VERSION     DAHHOJ010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5555
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.135"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            11..310
                     /locus_tag="NBH32_002377"
     CDS             11..310
                     /locus_tag="NBH32_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002433873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515947.1"
                     /translation="MMILLTIFCCLVLNKWFVSAVITFVILGVLAFFIPNFQDIKYQP
                     LLGYAAFLEIMSLLISFLLWYFTRNWRKERKARKLEKQIEKYGYEGAELRRKDKK"
     gene            258..443
                     /locus_tag="NBH32_002378"
     CDS             258..443
                     /locus_tag="NBH32_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515948.1"
                     /translation="MKNTAMRALNFAVKIKNNIRQLYAQNHRLLSYTAVTIISKEMEY
                     SAIVVFVLTFLYFTWLT"
     gene            614..1600
                     /locus_tag="NBH32_002379"
     CDS             614..1600
                     /locus_tag="NBH32_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515949.1"
                     /translation="MFIIKSMMYRLIQMIVVLFVISTLTFILMKLSPGNPVDKILHLD
                     VAQVSTEQINATKDKLGLNDSLLVQWWHWMNHLLHFNLGKSFESKEPVTQILFNYAPI
                     TLLISFSTLVVSLCISIPLGIIAAKRFHKWTDKVIRVISTLSISLPAFFIGIILLFIV
                     TNLMNIDSVILSQFILPVLTLSLGMCAYIIRLVRSNLLMLLQSNIVQASRLRGMNERY
                     ILIHDLLKPTIFPIIPLLGISLGSLIGGTVVIENLFDIPGIGYLLMDSIKSRDYPVIQ
                     GCVLFIGFFVVIINTIADLLTLLLDPKQRLQLGNPKNKTNTPLISEGSDRHA"
     gene            1593..2423
                     /locus_tag="NBH32_002380"
     CDS             1593..2423
                     /locus_tag="NBH32_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1515950.1"
                     /translation="MHKIFSKNNLIFFVFVAFIFVVIVLQFFVSSENTTKVNLSQTFE
                     PISWLHLLGTDDYGRDLFTRIIIGARSTLFVTVLTLIAIVVIGVTLGLFAGYKKGWIE
                     RLVLRFIDVGLSIPEFIIMIALASFFQPSLWNLVISIAVIKWMNYTRLTRSIVNSEMN
                     KPYIKMAQLFHVPTRTILIRHLTPKIIPAIIVLMVVDFGKIILYISSLSFIGLGAQPP
                     TPEWGAMLQQGRDFISSHPIMLIAPASVIAITILIFNLTGDTLRDRLLKQRGEYDESH
                     "
     gene            2410..3183
                     /locus_tag="NBH32_002381"
     CDS             2410..3183
                     /locus_tag="NBH32_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515951.1"
                     /translation="MSLIDIQNLTIKNTSEKSLIKGIDLKIFSQRINALIGESGAGKS
                     LIAKALLEYLPFDLTCTYDSYQFDGENVSKLSQYYGHTIGYISQNYAESFNDHTKLGK
                     QLTAIYRKHYKNSKEEALSKIDKALSWVNLQSKDILNKYSFQLSGGQLERVYIASVLM
                     LEPKLIIADEPVASLDALNGNQVMDLLQHIVLEHGQTLFIITHNLSHVLKYCQYINVL
                     KEGQIIERGNINHFKYEHLHPYTERLIKYRTQLKRDYYD"
     gene            3176..3877
                     /locus_tag="NBH32_002382"
     CDS             3176..3877
                     /locus_tag="NBH32_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1515952.1"
                     /translation="MIELKHVTFGYNKKQMVLQDINITIPDGENVGILGESGCGKSTL
                     ASLVLGLFKPVKGEIYLSDNAVLPIFQHPLTSFNPDWTIETSLKEALYYYRGLTDNTA
                     QDQLLIQHLSTFELNAQLLTKLPSEVSGGQLQRFNVMRSLLVQPRVLICDEITSNLDV
                     IAEQNVINILKAQTITNLNHFIVISHDLSVLQRLVNRIIVLKDGMIVDDFAIEELFNV
                     DRHPYTKELVQAFSY"
     gene            4001..4474
                     /locus_tag="NBH32_002383"
     CDS             4001..4474
                     /locus_tag="NBH32_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SWIM zinc finger family protein"
                     /protein_id="HCD1515953.1"
                     /translation="MGLLDIASIRSIERGFNYYQSECVINLKSFSETQHEAEVKGSGN
                     KVYRCYIDMEHPRKSKCNCPHADGRRVICKHMIALLFTASPEAANKHIIMLNEVEEDY
                     HLRRNMWIDSLKEMINDMSEEELRDAYLNMLIEHGEMAELFGLDEEDEMFEDEFY"
     gene            complement(4523..5290)
                     /locus_tag="NBH32_002384"
     CDS             complement(4523..5290)
                     /locus_tag="NBH32_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1515954.1"
                     /translation="MRFVFDIDGTLCFDGRLIDQTIIDTLLQLQHDGHELIFASARPI
                     RGLLPVLPSVFHRHTLIGANGAMISQQSKISVIKPIHTDTYHHILKIIQKYELDFIID
                     DDWNYAAQLDAENAIFERLDPHKLASCIDVANIDTPIKVILLNIDPAQITTILDELNK
                     YHQELEMIHHSNEYNIDITAQNINKYTALQYIFDADVKYIAFGNDHNDIVMLQHASSG
                     YIIGPSEAYTHAILKLDKIKHINNNAQAICKVLKSYK"
BASE COUNT         1883 a          766 c          950 g         1956 t
ORIGIN      
        1 tacaacatta ataatgattt tattaacaat attctgttgc ttagttttaa ataaatggtt
       61 tgtatctgca gtaattacat ttgttatatt aggtgtgctt gcatttttta ttccaaattt
      121 tcaagatata aaatatcaac cattattagg atacgctgca tttttagaga ttatgagttt
      181 attaattagt tttctattat ggtattttac tagaaattgg cgtaaagaaa gaaaagcgcg
      241 taaattagaa aagcaaattg aaaaatacgg ctatgagggc gctgaacttc gccgtaaaga
      301 taaaaaataa tattcgacaa ctatatgcac aaaatcatcg acttttaagt tacactgcag
      361 taacaattat ttcgaaagag atggaataca gtgctatagt tgtttttgtt ttgacttttt
      421 tatattttac atggttgact tgattcaaaa tattatattt tataatgatt ttgttaaaaa
      481 attaaatata cttcaatttc ttttgggata atactataat gaacgttggt tcttaatatg
      541 taagttgcat atacacgcgc cattctcatg taacaagaaa gtgttggtaa cttacacaag
      601 gagggggact attatgttca taataaagtc tatgatgtat agactgatac aaatgatagt
      661 tgttttattt gttatttcta cacttacatt cattttaatg aaattatcac caggaaatcc
      721 tgttgataaa atattacatc ttgatgtagc acaagtgtcg acggaacaaa taaatgcaac
      781 caaagataaa ttgggattaa atgattcttt attggtacag tggtggcatt ggatgaatca
      841 tttattacat tttaatttag gaaaaagttt tgaatcaaaa gaaccggtaa cacagatatt
      901 attcaactat gcacccataa cattactcat atcattttca acattagtag tgtcgttatg
      961 catatctata ccgctcggca ttatagcagc caaacgattt cataagtgga cggataaagt
     1021 gattagagtg atctcaacac tatcaattag tttaccagcg ttctttatcg gtattatttt
     1081 attatttatt gtcacaaatt taatgaatat agatagcgtt atactaagtc agtttatatt
     1141 acctgtactc acactatctt taggtatgtg tgcatacatc attcgtttag tgcgctctaa
     1201 tttattgatg ttattgcaaa gtaatatcgt acaagcatca agattacgcg gtatgaatga
     1261 acgttatatt ttaattcatg atttactaaa accaacaatt tttccgatta tcccattatt
     1321 agggatttca cttggtagtc taataggtgg tactgtagtg attgaaaatt tatttgatat
     1381 acctggtatt ggttatctat taatggatag tattaaatct cgagattatc ctgttattca
     1441 aggatgcgtg ttatttattg gcttcttcgt tgttattatt aatacgattg ctgatttatt
     1501 aacgttatta cttgatccga agcagcgtct acaattagga aatccaaaaa acaaaaccaa
     1561 tacaccattg atatcagaag gtagtgaccg tcatgcataa aatattttca aagaataacc
     1621 tgatattttt tgtattcgtt gcatttattt ttgtggtaat tgtactgcaa ttctttgtaa
     1681 gtagtgaaaa tacaaccaaa gtcaatttat cacaaacttt tgaacctatt agttggttgc
     1741 atttattagg aactgatgat tatgggagag atttatttac ccgaattatt atcggtgcac
     1801 gttcaacatt gtttgttact gttttaacat taatagctat cgttgtcata ggtgttacac
     1861 taggtctatt tgccggatat aaaaaggggt ggattgaacg attagtgtta aggtttattg
     1921 atgttggtct aagtattcca gaatttatca tcatgattgc tttagcaagc tttttccaac
     1981 catcgttatg gaatttagtt atttcaattg cagtaataaa atggatgaat tacacaaggt
     2041 tgactagaag tatagttaat agcgaaatga ataagcctta tataaaaatg gcacaattat
     2101 ttcatgtacc tacaagaaca atattaatac gtcatttaac acctaaaatt ataccggcta
     2161 ttatcgtttt gatggtcgtt gatttcggta aaatcattct atatataagt tcactatcat
     2221 ttattgggtt aggtgcacaa ccgccaacac cagagtgggg cgctatgttg caacaaggtc
     2281 gtgattttat ttcgtctcat ccaattatgt tgattgcacc tgcttcagtc attgctataa
     2341 ctattttaat ttttaattta accggtgata cgctaagaga tagattgctg aaacaacgag
     2401 gtgaatatga tgagtctcat tgatatacaa aatttaacaa taaagaatac tagtgagaaa
     2461 tcacttatta aagggattga tttgaaaatt tttagtcaac ggattaatgc cttgattgga
     2521 gagagcggcg ctggaaaaag tttgattgct aaagccttac ttgaatattt accattcgat
     2581 ttaacctgca cgtatgattc gtaccaattt gatggggaaa atgttagtaa attgagtcaa
     2641 tattatggtc atacaattgg ctatatttct caaaattatg cagaaagttt taacgaccat
     2701 actaaattag gtaaacagtt aactgcgatt tatcgtaagc attataaaaa tagtaaagaa
     2761 gaggctttgt ccaaaattga taaggctttg tcgtgggtta atttacaaag caaagatata
     2821 ttaaataaat atagtttcca actttctggg ggccaacttg aacgcgtata catagcaagc
     2881 gttctcatgt tggagcctaa attaatcatt gcagacgaac cagttgcatc attggatgct
     2941 ttgaacggta atcaagtgat ggatttatta cagcatattg tattagaaca tggtcaaaca
     3001 ttatttatta tcacacataa cttaagtcat gtattgaaat attgtcagta cattaatgtt
     3061 ttaaaagaag gtcaaatcat tgaacgaggt aatattaatc atttcaagta tgagcatttg
     3121 catccgtata ctgaacgtct aattaaatat agaacacaat taaagaggga ttactatgat
     3181 tgagttaaaa catgtgactt ttggttataa taaaaagcag atggtgctac aagatatcaa
     3241 tattactata cctgatggag aaaatgttgg tattttaggc gaaagtggct gtggtaaaag
     3301 tacgctcgct tcattggttc ttggcttgtt taaacctgtt aaaggagaaa tttacttaag
     3361 tgataatgct gtgttaccga ttttccaaca ccctttaact agctttaacc ctgattggac
     3421 gattgagacc tcattaaaag aagcgttata ttattacaga ggcctaactg ataatactgc
     3481 tcaggatcaa ttattaatac aacatttatc tacttttgag ttgaacgcgc aattattgac
     3541 taaattacca agcgaagtga gtggtggaca attacaaaga tttaatgtca tgcgttcgtt
     3601 attagtacaa cctcgcgttt taatatgtga tgagataact tcaaatttag atgttatagc
     3661 tgaacaaaat gtaataaata tattaaaagc gcaaacgatt acgaacttaa atcattttat
     3721 cgttatttct catgatttat ccgtgttaca acgcttagtt aatagaatta tcgttcttaa
     3781 ggatggcatg atagtcgatg attttgcaat agaggaatta tttaatgttg atagacaccc
     3841 ttatacaaaa gaattagtgc aagcattttc atattagtta tttaagaatg cgataattct
     3901 agacttgtta taaaatatag ataaatcaag tatattaatc tagacactta tcgattttat
     3961 tttctgtatt taaaaataat aataaaaagg agtatcatta atgggattac ttgatattgc
     4021 aagtattcgt tctatagaaa gaggctttaa ttattatcaa agtgaatgcg tcattaactt
     4081 aaaatcattt tcagaaacgc agcatgaggc tgaagtaaaa ggcagtggta acaaagtata
     4141 tcgttgttat attgatatgg aacatcctag aaaatccaaa tgtaattgtc ctcatgctga
     4201 tggaagacga gtgatatgta aacatatgat tgcattactc tttacagcta gtccagaagc
     4261 agcaaataaa catataatca tgttaaacga agttgaagaa gactatcact tacgcagaaa
     4321 tatgtggatt gattctctta aagaaatgat taatgatatg agtgaagaag aactccgcga
     4381 tgcatattta aacatgttaa ttgaacatgg agaaatggca gaattatttg gattagatga
     4441 agaggacgaa atgttcgagg acgaatttta ttaaaatagc ccctatcgat tgataatgat
     4501 tatcatttga taggggtgtt ttttatttat atgattttaa gactttgcaa atagcttgtg
     4561 cattattatt gatgtgtttg attttatcaa gtttcaatat tgcgtgtgtg tatgcttctg
     4621 atggtcctat aatatagcca ctactagcat gttgtaacat gacaatatca ttgtggtcat
     4681 taccaaatgc tatatattta acatctgcat caaatatata ttgtaatgca gtatatttat
     4741 taatattttg cgctgttata tcaatgttat actcatttga atggtgaatc atttccaatt
     4801 cttgatggta tttatttagc tcgtctaata tagttgtaat ttgtgccggg tctatattta
     4861 ataaaataac tttgattggc gtgtcgatat ttgcaacatc aatacaactg gccagcttat
     4921 gtggatctaa acgctcaaaa atcgcgttct cagcgtcaag ttgtgcagca taattccaat
     4981 catcatcaat aataaaatct aactcatact tttgaattat ttttaagata tggtgatatg
     5041 tatcagtatg aattggtttg ataacagaaa tctttgattg ctgtgaaatc atagcaccat
     5101 ttgcgccaat taatgtgtgc cgatgaaata ctgatggtaa aactggcaac aaaccacgaa
     5161 ttggacgtgc tgatgcaaat ataagttcat gaccatcatg ttgtaattgt aacaatgtat
     5221 caataatagt ctgatcaatt aatcggccgt caaaacaaag cgtaccatca atatcaaaga
     5281 caaatctcat caaatcactc caaacaatat aataccatga ttatagcata agtaagttat
     5341 gtgacgatat tagataatga ttatgatgta gggtaccttt tgcgtcacac acattacttc
     5401 ctataatata tattatgtaa actagaatgt gaaattccat aaggggactt atataactgt
     5461 atgtttgtgt aactcgtttt gattattagt aaagtatgtt tgtagttact attaactttt
     5521 tatttaacac atgctatttt atcaatactt ctatt
//
LOCUS       DAHHOJ010000135         5516 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.136, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000135 DAHHOJ010000000
VERSION     DAHHOJ010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5516)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5516)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5516
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.136"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            62..634
                     /locus_tag="NBH32_002385"
     CDS             62..634
                     /locus_tag="NBH32_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000540837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCD1515955.1"
                     /translation="MGYKRKTVLTQRNIKNALVSLLENNKFDFITINQIVEEAEITRS
                     TFYRYYDDKYELISEIEEEILEHIHKERQKIDENASEKNLLDIEMFKYLFETLEPYSN
                     TIYRLLGYNGDSSFEMKLKNEISRRFIDIEQRNHISQVRADLVKEYVFVIMIKTLQYW
                     SAHKDEVDIDEIALTIRDIQLKGLRKAIGL"
     gene            complement(692..1678)
                     /locus_tag="NBH32_002386"
     CDS             complement(692..1678)
                     /locus_tag="NBH32_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000662030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HCD1515956.1"
                     /translation="MKENFWSELPRPFFILAPMEDVTDIVFRHVVSEAARPDVFFTEF
                     TNTESFCHPEGIHSVRGRLTFSEDEQPMVAHIWGDKPEQFRETSIQLAKMGFKGIDLN
                     MGCPVANVAKKGKGSGLILRPDVAAEIIQATKAGGLPVSVKTRLGYYEIDEWKDWLKH
                     VFEQDIANLSIHLRTRKEMSKVDAHWELIEAIRNLRDEIAPNTLLTINGDIPDRKTGL
                     ELAEKYGIDGVMIGRGIFHNPFAFEKEPREHTSKELLDLLRLHLSLFNKYEKDEIRQF
                     KSLRRFFKIYVRGIRGASELRHQLMNTQSIAEARALLDEFEAQMDEDVKIEL"
     gene            1987..2190
                     /locus_tag="NBH32_002387"
     CDS             1987..2190
                     /locus_tag="NBH32_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515957.1"
                     /translation="MEDLKHSLKSLGWWDLFFAIPMFLLFAYLPNYNFITIFLNIVII
                     IFFSIGLILTTHIIIDKIKSSTK"
     gene            2239..2535
                     /locus_tag="NBH32_002388"
     CDS             2239..2535
                     /locus_tag="NBH32_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000179889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HCD1515958.1"
                     /translation="MATKKDVHDLFLNHVNSNAVKTRKMMGEYIIYYDGVVIGGLYDN
                     RLLVKATKSAQQKLQDNTLVSPYPGAKEMILIPDFDEATNLTDLFKTIKNDLKK"
     gene            complement(2599..3820)
                     /locus_tag="NBH32_002389"
                     /pseudo
     CDS             complement(2599..3820)
                     /locus_tag="NBH32_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498648.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(3813..4364)
                     /locus_tag="NBH32_002390"
     CDS             complement(3813..4364)
                     /locus_tag="NBH32_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCD1515959.1"
                     /translation="MITCLFGSSRKDGNSAIAVENLLKNLNVNFVNLYQSNIEKVTDN
                     RHSKGANFHNDDYEQILIKVLESDIIIFSTPLYWYSMSASLKLFIDRWTESLRDTQID
                     NFKEIMSQKKYLILIIGGDNPHTKAQPLVNQFKLIFEFMNITNYRFLIGEGNKPFDIL
                     NDSQFIEELANINLALKKGDIYD"
     gene            4477..5337
                     /locus_tag="NBH32_002391"
     CDS             4477..5337
                     /locus_tag="NBH32_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1515960.1"
                     /translation="MNLNILKSFLVTSETKNLTKASELLNYSQSTVSTHIEKLERQLD
                     VKLFYRKKYGMELTEEGLAYVKYAKVILDSNSEYEREIKGLYNKKVNISINMQESQYL
                     YRYYNKISEWLAEHPYVNLKFKSAHSNFYIKEEIANFKSDISLITDEKIINSNLTAIP
                     ITEERLIFVTNKEMKDFKLNDIQNHTLLVTEKGCSYREQLEKILYKYSFSTKQMIEFL
                     GIESLKKHLKNSDGIALLPEFIVADELQNKSLFQIDVDVDIPNLETTLIINPESSKQV
                     LESFVKDVFL"
BASE COUNT         1971 a          814 c          877 g         1854 t
ORIGIN      
        1 tttgtacaat ttgtgacact ttgttttatt ttgtgttata ttcatttata ggagtgatca
       61 tatggggtat aaaaggaaaa cggtcttaac tcagcgtaat ataaaaaatg ctttagtgag
      121 tttattagag aacaataagt ttgatttcat tacaatcaat caaatagtag aagaagcgga
      181 aataacaaga agtacttttt acaggtatta tgatgataaa tatgaattaa tatcagaaat
      241 agaagaagaa attttagaac atattcataa agaaaggcaa aagatagacg agaacgcttc
      301 agagaagaat ttattagata ttgagatgtt taaatatctg tttgaaacat tagagccata
      361 ttcgaacacg atatatcgat tgttaggata taatggtgat tccagttttg agatgaaact
      421 caagaatgaa atatcaagaa gatttataga tatagaacaa cgtaatcata tttctcaagt
      481 acgagcagac ttagtaaagg aatatgtatt tgttattatg ataaaaacat tacaatattg
      541 gtcagctcat aaagacgaag tagatattga tgaaatagct ttaacaatta gggatatcca
      601 gttaaaagga ttaagaaaag caatagggtt ataattttaa tgaataacaa aaaaggagga
      661 acattatatt cctccttgac cttcattaca tctataattc aattttaacg tcttcgtcca
      721 tttgggcttc aaattcatct agaagtgctc gtgcttctgc aattgattgt gtgttcatca
      781 attgatggcg aagttcgcta gcgcctctta tgccacgcac atagatttta aagaatctac
      841 gcaagctctt gaattgtcgt atttcatctt tctcatattt gttaaacaat gataaatgta
      901 atctcaacag atctaatagt tctttgcttg tgtgttcgcg tggttctttt tcaaaagcga
      961 atggattatg gaaaatgcct ctaccaatca tgacgccatc aatgccatat ttttctgcaa
     1021 gttcaagtcc tgtttttcta tcgggaatat caccgttaat tgttaacaat gtatttggtg
     1081 caatttcgtc acgtaaattt ctaatagctt cgattaattc ccaatgtgca tctactttac
     1141 tcatttcttt acgtgtacga agatgaatag ataaattggc aatatcttgt tcgaagacgt
     1201 gcttcaacca atctttccat tcatcgattt catagtagcc aaggcgtgtt ttaacactta
     1261 ccggaagtcc acctgcttta gttgcttgaa taatttcggc agcgacgtca ggtcttaaga
     1321 ttaagccgga acccttaccc tttttagcaa catttgctac aggacatccc atatttaagt
     1381 ctattccttt aaagcccatt ttagctaatt gaatactcgt ttcacggaac tgttctggct
     1441 tatctcccca tatatgagcg accatcggct gttcatcttc gctaaaagtt aagcgtccac
     1501 gcacactatg tatgccttca gggtggcaaa agctctcggt atttgtaaat tcagtgaaaa
     1561 acacatccgg tctagctgct tcacttacaa cgtgtcgaaa gacgatatct gtaacgtctt
     1621 ccattggcgc caaaataaaa aatggacgtg gtaattcact ccaaaaattt tctttcataa
     1681 tatatttata ccctctttat aattagtatc tcgatttttt atgcatgatg atattaccac
     1741 aaaagacgaa cttatacaaa aggaatttta gttgatacaa ccatttgaaa gggaagtcta
     1801 agagtagtct aaaatgaatg ttgtggtaag ttgatcagta tacagatcaa ggattatcgt
     1861 attagattgt tcattattaa tgatacacta cttatgaaaa tgattcagaa ttttctttgg
     1921 ctacttttac agtaaagcga ttttttagtt atcttataac aaagacaaat ttataaaggt
     1981 gatattatgg aagatttaaa gcattcttta aaaagtttag gttggtggga tttatttttt
     2041 gcgataccta tgtttctgct attcgcatac cttccaaact ataattttat aacgatattt
     2101 cttaacattg ttatcattat tttcttttcc ataggtttga ttttaactac gcatataatt
     2161 atagataaaa ttaagagcag cacgaaatga ttcattaata cgaatgtgat taaacataaa
     2221 actgaaggag cgattacaat ggcgactaag aaagatgtac atgatttatt tttaaatcat
     2281 gtgaattcaa acgcggttaa gacaagaaag atgatgggag aatatattat ttattatgat
     2341 ggcgtggtta taggtggttt gtatgataat agattattgg tcaaggcgac taaaagtgct
     2401 cagcagaaat tgcaagataa tacattagtt tccccgtacc caggtgctaa agaaatgata
     2461 ttaattccag actttgacga agcaacaaat ctcactgatt tatttaagac cataaaaaat
     2521 gatttgaaaa agtgaagtgt gggtgcagag agaactaagc ccatcgataa atggtcgctt
     2581 gttaaagaag agtgaccgtc actcttcttt atgtgcatat tttattttgt ctgttttgtt
     2641 aacgatcagc agtgtaacaa atatgagtaa ggataaaatg agtataatat agaaaccgaa
     2701 tttatcatta atttcattaa tccatcttcc taaaaatgga gcaatgaaac tctgcagtaa
     2761 caatgaaatt gacgtccata tcgtaaatga gcgaccgaca tatttatctg aaacagtgtt
     2821 cattatagct gtattcatat aaattctgat tgatgaaatt gagtagccta gtataaatga
     2881 tcctatgaat aagtaaaatg ctgagtttat ccaaataaat agtgctgaat ttatgactaa
     2941 tatgaaatat aacaaaaata tcaatacttt agttgagatt ttcttcgaaa gaatagctga
     3001 aattaaacct gcacataatc ctccaatgcc atataacata tctgaaaaac caaattgtac
     3061 agacgaaatt ttttaaaaca ttatatacat atcctggtaa tgatatgtta aagatcattg
     3121 taaacaccat tggtatgatt gaaataactc caaaaataaa tatcatcatg ttgtctttta
     3181 aaaatttcca tcctaataaa tattcttgca ataagctatt tgttgattct tcctctgaat
     3241 gagttggttt atctacatgc aatctaaata acataaaaat gctgattaga aacatcatta
     3301 tagtcatcgc tataattaga gtgaatccat ttatctcata taatattcct gataatccac
     3361 ctgcaataaa catacctgtt tgcaaactaa tctctaatag agaatttgca tctgtatatt
     3421 gatctggttt taaaatctgt ttaactaaac ttctagatgt tgccatatac gtagtccaac
     3481 ctatcccatt aacaatcgca aatccaatca ctaaataggt ctcaaatcct atcattacga
     3541 gtgctatgac aatgagtaaa tataatatta cctgaagaag ataggtgatt aaaataatat
     3601 ttcgtctatt atatttatcg gctagtccac ctattatagg agaagctaag aaaccggata
     3661 atacatttaa agctaacata atacctaata gttgggagtc gttagttttg tcgattaaat
     3721 accaattagc cccaactgta ctaattccaa ctccgaatgc tgaaattatt tctgctaaaa
     3781 agaacaatct gaagttttta attttcaaca aatcagtcat aaatatcacc ttttttcaat
     3841 gcaagattta tatttgcgag ctcttctata aattgagagt cgtttaaaat atcgaatggt
     3901 ttattgcctt cgccgatcaa aaatctatag ttagtaatat tcataaactc aaatattaat
     3961 ttgaattggt tgactaaagg ttgtgctttg gtatgtggat tgtcgccacc tatgattaaa
     4021 atcaaatact ttttttgtga catgatttct ttgaagttgt ctatttgcgt atctcgcaat
     4081 gactcggtcc atctatcaat gaagagtttt aaagacgcac tcatggagta ccaatagagt
     4141 ggtgttgaaa aaataataat atctgattct aaaactttga ttaaaatttg ttcgtaatca
     4201 tcattatgaa agttggcacc tttgctatga cgattatctg taaccttttc aatgttgctt
     4261 tgatataaat tcacaaagtt gacatttaaa ttcttcaata gattctctac tgcgatagct
     4321 gaattgccat cttttctact acttccaaat aaacaagtaa tcatagtcat aactcctttc
     4381 gatttacctt tataataata tttaatatat tattattaaa tcagaattct tagaattcag
     4441 gattcgataa aagtgaatcc taaaggaggt actactttga acttaaacat tttaaaatca
     4501 tttttagtaa ctagtgaaac aaagaacctt actaaagcat cagagttact taactattca
     4561 cagtcaactg tatctacaca tattgaaaaa ttagaaaggc aattagatgt taaattattt
     4621 tatagaaaaa aatatggtat ggaactaacg gaagaaggct tagcatacgt taaatacgct
     4681 aaagtgattt tagatagtaa tagcgaatac gagagagaaa taaaaggact ttacaataag
     4741 aaggtaaata taagtattaa catgcaagaa agtcagtatt tgtatcgcta ctataataag
     4801 attagtgaat ggttagctga acacccatat gtaaacttga agtttaaatc cgcacattct
     4861 aatttctata ttaaagaaga aattgctaat ttcaaatcgg atattagcct tatcacagac
     4921 gaaaagatta ttaatagtaa cttaactgct attcctataa ctgaagaacg tttaatattt
     4981 gttactaaca aagagatgaa agatttcaaa ttaaacgata ttcaaaacca tacattactt
     5041 gttactgaaa aagggtgtag ttacagagaa cagttagaaa agattctcta taaatatagc
     5101 ttttcaacaa aacaaatgat tgaatttcta ggaattgagt cattgaaaaa acacttgaaa
     5161 aattcagatg ggattgcgct attgccggag tttattgttg cagatgaatt acaaaataaa
     5221 agtttgtttc aaatagatgt cgatgtcgat attccgaatt tagaaacaac tttgataatt
     5281 aatcctgaat ccagtaagca agtactagaa tcttttgtaa aagatgtctt tttataaata
     5341 ttggtgaaaa cgtgtagtta tggtgaaact caaagataat aatttaaatg agatgttaat
     5401 gaaaaagtaa ttcaatataa aaacaggtga tttatatctt taataaggat tatttctagt
     5461 tgaaatttca attgcgggca ttcattttca aaatagtaaa gaatttaata gatgaa
//
LOCUS       DAHHOJ010000136         5396 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.137, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000136 DAHHOJ010000000
VERSION     DAHHOJ010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5396
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.137"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            155..1273
                     /gene="ald"
                     /locus_tag="NBH32_002392"
     CDS             155..1273
                     /gene="ald"
                     /locus_tag="NBH32_002392"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HCD1515961.1"
                     /translation="MLVAVVKELKQGEGRVACTPENVRKLTDAGHKVIVEKNAGIGSG
                     FSDDMYEKEGAKIVTHEQAWEADLVIKVKEPHTSEYQYFKKDQIIWGFLHLASSKEIV
                     EKMQEVGVTAISGETIIKNGKAELLAPMSAIAGQRSAIMGAYYSEAQHGGQGTLVTGV
                     HENVDIPGSTYVIFGGGVAATNAANVALGLNAKVIIIELNDDRIKYLQDMYAEKDVTV
                     VKSTPENLAEQIKKADVFISTILIPGAKPPKLVTREMVKSMKKGSVLIDIAIDQGGTI
                     ETIRPTTISDPVYEEEGVIHYGVPNQPGAVPRTSTMALAQGNIDYILEICDKGLEQAI
                     KDNEALSTGVNIYQGQVTNQGLATSHDLDYKEILNVIE"
     gene            1368..2408
                     /gene="tdcB"
                     /locus_tag="NBH32_002393"
     CDS             1368..2408
                     /gene="tdcB"
                     /locus_tag="NBH32_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCD1515962.1"
                     /translation="MTTNTVTLQTAHIVSLGDIEEAKASIKPFIRRTPLIKSMYLSQS
                     ITKGNVFLKLENMQFTGSFKFRGASNKINHLTDEQKEKGIIAASAGNHAQGVALTAKL
                     LGIDATIVMPETAPQAKQQATKGYGAKVILKGKNFNETRLYMEELAKENGMTIVHPYD
                     DKFVMAGQGTIGLEILDDIWNVNTVIVPVGGGGLIAGIATALKSFNPSIHIIGVQSEN
                     VHGMAESFYKRDLTEHRVDSTIADGCDVKVPGEQTYEVVKHLVDEFILVTEEEIEHAM
                     KDLMQRAKIITEGAGALPTAAILSGKINNKWLEDKNVVALVSGGNVDLTRVSGVIEHG
                     LNIADTSKGVVG"
     gene            2439..3761
                     /locus_tag="NBH32_002394"
     CDS             2439..3761
                     /locus_tag="NBH32_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1515963.1"
                     /translation="MSNGKELQKNIGFFSAFAIVMGTVIGSGVFFKISNVTEVTGTAG
                     MALFVWFLGGIITICAGLTAAELAAAIPETGGLTKYIEYTYGDFWGFLSGWAQSFIYF
                     PANVAALSIVFATQLINLFHLSIGSLIPIAIASALSIVLINFLGSKAGGILQSVTLVI
                     KLIPIIVIVIFGIFQSGDITFSLIPTTGNSGNGFFTAIGSGLLATMFAYDGWIHVGNV
                     AGELKNPKRDLPLAISVGIGCIMAVYLLINATFLLTLPIELLAGNLNAASDTSKILFG
                     ENGGKIITIGILISVYGTINGYTMTGMRVPYAMAERKLLPFSHLFAKLTKSGAPWFGA
                     IIQLIIAIIMMSMGAFDTITNMLIFVIWLFYCMSFVAVIILRKREPNMERPYKVPLYP
                     IIPLIAILAGSFVLINTLFTQFILAIIGILITALGIPVYYYKKKQKAA"
     gene            3917..5308
                     /gene="norB"
                     /locus_tag="NBH32_002395"
     CDS             3917..5308
                     /gene="norB"
                     /locus_tag="NBH32_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000414682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter NorB"
                     /protein_id="HCD1515964.1"
                     /translation="MEKPSREAFEGNDKLLIGIVLSVITFWLFAQSLVNVVPILEDSF
                     NTDIGTVNIAVSITALFSGMFVVGAGGLADKYGRIKLTNIGIILNILGSLLIIISNIP
                     LLLILGRLIQGLSAACIMPATLSIIKSYYIGKDRQRALSYWSIGSWGGSGVCSFFGGA
                     VATLLGWRWIFILSIIISLIALFLIKGTPETKSKSISLNKFDIKGLVLLVIMLLSLNI
                     LITKGSELGVTSLLFITLLAIAIGSFSLFIVLEKRATNPLIDFKLFKNKAYTGATASN
                     FLLNGVAGTLIVANTFVQRGLGYSSLQAGSLSITYLVMVLIMIRVGEKLLQTLGCKKP
                     MLIGTGVLIVGECLISLTFLPEILYVICCVIGYLFFGLGLGIYATPSTDTAIANAPLE
                     KVGVAAGIYKMASALGGAFGVALSGAVYAIVSNMTNIYTGAMIALWLNAGMGILSFVI
                     ILLLVPKQNDTQL"
BASE COUNT         1891 a          767 c         1021 g         1717 t
ORIGIN      
        1 tgcacataat atttaagaat aagaataata tattaaatat ttattaacaa attatagata
       61 aaatatgaat aattaattaa tggtatttac atatgcatac ccacaagata aaatgattgt
      121 gaaataattc acaaaatcaa aaggagtgtt gcaaatgtta gttgcagtag tcaaagaatt
      181 aaaacaaggt gaaggacgtg tagcttgcac acccgaaaat gtgcgtaagt taacggatgc
      241 aggacataaa gtaattgtag aaaaaaatgc tggcattggt tcaggatttt ctgacgatat
      301 gtatgaaaaa gaaggcgcta agattgtaac tcacgaacaa gcatgggaag cagatctggt
      361 tatcaaagta aaagaaccac atacaagtga atatcaatat tttaaaaaag atcaaatcat
      421 ctggggattc ttacatttag catcttcaaa ggaaatagta gaaaaaatgc aagaagttgg
      481 tgttactgct attagtggtg aaaccattat aaaaaatgga aaagcagaat tattagcgcc
      541 aatgagtgct atagcaggtc aacgctcagc aattatggga gcttactact ctgaagcaca
      601 acatggtggt caaggtactt tagtgactgg tgtacatgaa aatgtggata tacctggtag
      661 tacatatgtg attttcggtg gtggcgtagc agcaacaaat gcagcaaatg ttgccttggg
      721 actaaatgct aaagtaatca ttatcgagtt aaacgatgat cgcattaaat atttacaaga
      781 catgtatgca gaaaaagatg tcacagtagt caaatcaaca ccagaaaatt tagcagaaca
      841 aataaaaaaa gcagatgtat ttatttctac aattttaatt ccaggtgcga aaccgccaaa
      901 attggttact cgtgagatgg ttaaatcaat gaaaaaaggt tcagtattaa tcgatatagc
      961 tattgaccaa ggtggaacta ttgaaacaat tagaccaact acaatttctg atccagtgta
     1021 tgaagaagaa ggtgtgattc attacggtgt accaaaccaa ccaggagcag tcccaagaac
     1081 ttcaacaatg gcattagcac aaggaaatat tgattatata ttagaaattt gtgacaaagg
     1141 cttagaacaa gcaattaaag ataatgaagc cttaagtact ggtgtaaaca tttaccaagg
     1201 acaagtgaca aatcaaggat tagctacatc acatgaccta gattataaag aaatattaaa
     1261 tgttatcgaa tagatagtaa tttaaatgaa attgagtgaa atgaatattt taaatatagc
     1321 attatagttt ggactaaaaa tttacaaaac ggaaggatgt aattattatg acaaccaaca
     1381 cagttacatt acaaacagca catattgtaa gtttaggaga tatcgaagaa gctaaagcaa
     1441 gcattaaacc atttattcgt cgaacacctc taattaaatc aatgtattta agccaaagta
     1501 taactaaagg gaatgtattt ctaaaattag aaaatatgca attcacagga tcttttaaat
     1561 ttagaggcgc tagcaataaa attaatcact taacagatga acaaaaagaa aaaggcatta
     1621 tcgcagcatc tgctgggaac catgcacaag gtgttgcttt aacagctaaa ttattaggca
     1681 ttgatgcaac tatcgtaatg cctgaaacag caccacaagc aaaacaacaa gcaacaaaag
     1741 gctatggggc aaaggttatt ttaaaaggta aaaactttaa cgaaactaga ctttatatgg
     1801 aagaattagc gaaagaaaat ggcatgacaa tcgttcatcc atatgacgat aagtttgtaa
     1861 tggcaggcca aggaacaatt ggtttagaaa ttttagatga tatttggaat gtgaatacag
     1921 tcatcgtacc agttggcggt ggaggattaa ttgcaggtat tgccaccgca ttaaaatcat
     1981 ttaacccttc aattcatatt atcggtgttc aatctgagaa tgttcatggt atggctgagt
     2041 ctttctataa gagagattta actgaacatc gagtggatag cacaatagca gatggttgtg
     2101 atgtaaaagt tcctggtgaa caaacatatg aagtagttaa acatttagta gatgaattta
     2161 ttcttgttac tgaagaagaa attgaacatg ctatgaaaga tttaatgcag cgtgccaaaa
     2221 ttattactga aggtgcaggc gcattaccaa cagctgcaat tttaagtgga aaaataaaca
     2281 ataaatggct tgaagataaa aatgttgttg cattagtttc aggcgggaat gttgacttaa
     2341 ctagagtttc aggtgtcatt gaacatggac tgaatattgc agatacaagc aagggtgtgg
     2401 taggttaaaa catttaatct taaaaatgag gtgtaattat gtcaaatggt aaagaattac
     2461 aaaaaaatat aggtttcttc tcagcgtttg ctattgttat ggggacagtt attggttcag
     2521 gagtattctt taaaatatca aacgtaacag aagtaacagg aacagcagga atggccttgt
     2581 ttgtatggtt cctaggcggc atcattacca tttgtgcggg gttaacagca gcagaacttg
     2641 ctgctgcaat ccctgaaaca ggtggcttaa cgaagtatat agaatataca tacggtgatt
     2701 tctggggctt cttatcaggc tgggcacaat catttattta ttttccagca aacgtagcag
     2761 cattgtctat cgtatttgcg acacagttaa ttaatttatt ccatttatct ataggttcat
     2821 taataccaat agcaatcgca tctgcgttat ctattgtgtt gataaatttc ctaggttcaa
     2881 aagcaggtgg aattttacaa tcagttacat tagtaattaa actgattcca atcatcgtta
     2941 tcgtaatttt tggtattttt caatctggag atatcacttt ttcattaatt ccaactacag
     3001 gtaattcagg aaatggtttc tttacagcaa ttggtagtgg tttattagca actatgtttg
     3061 catatgatgg ttggattcat gtaggaaatg ttgcggggga acttaaaaat cctaaacgcg
     3121 atttaccttt agcgatttca gttggtatcg gttgtattat ggctgtgtat ttattaatta
     3181 acgctacatt cttattaacg ttgccaattg aactacttgc tggtaattta aatgcagctt
     3241 cagatacatc aaaaatatta tttggtgaaa atggcggtaa gattattaca attggtatat
     3301 taatttctgt ttatggtacg atcaatggct atactatgac tggtatgcgc gtaccatatg
     3361 caatggctga aagaaaatta ttgccattta gccacttatt cgcaaaatta acaaaatctg
     3421 gcgcaccatg gtttggcgca attatacaac ttataatcgc tatcatcatg atgtcaatgg
     3481 gagcatttga tacaattaca aacatgttaa tctttgttat ttggttgttc tattgtatgt
     3541 catttgttgc ggtaataatt ttaagaaaac gtgaaccaaa tatggagcga ccatataaag
     3601 taccgttata tccaatcata cctttaattg ctattttggc aggatcattt gtattaatta
     3661 atacactgtt tacacaattt atactagcaa ttattgggat tctaatcaca gcacttggta
     3721 taccagttta ttactataag aagaaacaaa aagcagcata aggtaagata actagcattg
     3781 agaataaatg gatggactac taataaattt aaagttttac acattaaaat caaaaaccat
     3841 tcaattattc tatcgaacag acaaatttct gttatggaat ttgtctgttt tcaaaagtat
     3901 agggaggcaa atagagatgg aaaagccgtc aagagaggca tttgaaggca atgataagtt
     3961 gttaatagga attgttttaa gtgtaataac gttttggcta tttgcacaat cattggttaa
     4021 tgttgtacca atacttgaag atagtttcaa tacagatatt ggaacggtaa atatcgccgt
     4081 tagtataact gctttatttt caggaatgtt tgtagtagga gcaggtggtc ttgctgataa
     4141 atatggcaga attaaactca cgaacattgg tattatctta aatatattgg gttcattatt
     4201 aatcattatt tcaaatattc ctttattact tattttagga agattaattc aaggactttc
     4261 agcagcatgt attatgcctg caactttgtc tattattaag tcatattaca ttgggaaaga
     4321 tagacaacgc gctttaagtt attggtcaat tggctcatgg ggcggctcag gtgtttgttc
     4381 attttttgga ggtgcagttg caacgctttt aggttggcgt tggattttca tcctatcaat
     4441 tataatttca ttaattgcgc tatttcttat taaaggcaca cctgaaacta aatctaaatc
     4501 gatttctcta aataaatttg acattaaagg tctggttctt ttagtcatta tgctcctcag
     4561 tttaaacatt ttaattacta aaggatcaga attaggtgta acctcacttc tttttatcac
     4621 tttattagca attgcaattg gatcttttag tttatttata gttcttgaaa agcgtgctac
     4681 aaatccttta atcgatttta aattatttaa aaataaagct tatacaggtg caacagcttc
     4741 aaactttttg ttaaatggtg ttgcgggaac attaatagta gccaacacat ttgttcaaag
     4801 aggtttagga tattcttcat tgcaagcagg aagtttatca atcacttatt tagtaatggt
     4861 actaattatg attcgtgttg gtgaaaagtt acttcaaaca ctcggatgca agaaaccaat
     4921 gttaattgga acaggagttc ttattgtcgg agaatgtctc atttcattaa catttttgcc
     4981 agaaatattg tatgtcattt gttgtgttat aggttattta ttctttggtt taggactagg
     5041 aatatatgct acaccatcaa cagatacagc aattgcaaat gcaccgttag aaaaagtagg
     5101 cgttgctgca ggtatctata aaatggcttc tgcattaggt ggagcatttg gcgtcgcatt
     5161 aagtggtgca gtttatgcaa tcgtatcaaa tatgacaaac atttatacag gtgcaatgat
     5221 tgcattatgg ttaaacgcag gtatgggaat tttatcattc gttatcattt tgttacttgt
     5281 gcctaaacaa aacgacactc aattatgata attgagaatt aaattgaaat catataagtc
     5341 gctacaatat taaacaaaaa tataaaccga ttcttatgtg tcattatttt aaatga
//
LOCUS       DAHHOJ010000137         5147 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.138, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000137 DAHHOJ010000000
VERSION     DAHHOJ010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5147)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5147)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5147
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.138"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..431)
                     /locus_tag="NBH32_002396"
     CDS             complement(<1..431)
                     /locus_tag="NBH32_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heptaprenyl diphosphate synthase component 1"
                     /protein_id="HCD1515965.1"
                     /translation="METTVSKLERQIEERLKGVSEYESININHRLGKLLDSYDIPDVA
                     KVACLTIDTSMRHLDDITYNHLSKHSILIGDLISAHFYTLLAEINDLSFQNEISKAIV
                     EINELKSSLHHQALNDYEISQAIVKIETLFPYITLSHFGIN"
     gene            complement(862..1134)
                     /locus_tag="NBH32_002397"
     CDS             complement(862..1134)
                     /locus_tag="NBH32_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015900354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HCD1515966.1"
                     /translation="MNKTDLINAVAEQADLTKKEAGSAVDAVFESIQNSLAKGEKVQL
                     IGFGNFEVRERAARKGRNPQTGKEIDIPASKVPAFKAGKALKDAVK"
     gene            complement(1305..2303)
                     /locus_tag="NBH32_002398"
     CDS             complement(1305..2303)
                     /locus_tag="NBH32_002398"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCD1515967.1"
                     /translation="MTKITVFGMGSFGTALANVLAENGHDVLMWGKNQDAVDELNTCH
                     TNKKYLKYAKLDVNIIATSDMTKAIQFADIYLMALPTKAMREVATQINDKLTSKKTFI
                     HVAKGIENGTFKRVSEMIEDSISPEYNAGIGVLSGPSHAEEVVVKQPTTVAASSKDKS
                     VSKLTQDLFMNDYLRVYTNDDLIGVELGGALKNIIAVASGIVAGIGYGDNAKAALMTR
                     GLAEISRLGEKLGADPMTFLGLGGIGDLIVTCTSTHSRNFTLGYKLGQGESMDQALSE
                     MNMVVEGIYTTKSVYHLAKEKNVDMPITNALYRVLFENISVKECVKDLMERDKKSE"
     gene            complement(2320..3630)
                     /gene="der"
                     /locus_tag="NBH32_002399"
     CDS             complement(2320..3630)
                     /gene="der"
                     /locus_tag="NBH32_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019167598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCD1515968.1"
                     /translation="MTKPIVAIVGRPNVGKSTIFNRIVGERVSIVEDTPGVTRDRIYS
                     SGEWLTHDFNIIDTGGIEIGDAPFQTQIRAQAEIAIDEADVIIFMVNVREGLTQSDEM
                     VAQILYKSKKPVVLAVNKVDNMEMRTDVYDFYSLGFGEPYPISGSHGLGLGDLLDAVV
                     SHFGEEEEDPYDEDTIRLSIIGRPNVGKSSLVNAILGEDRVIVSNVAGTTRDAIDTEY
                     SYDGQDYVLIDTAGMRKKGKVYESTEKYSVLRALKAIERSNVVLVVIDAEQGIIEQDK
                     RVAGYAHEQGKAVVIVVNKWDTVEKDSKTMKKFEDEVRKEFQFLDYAQIAFVSAKERT
                     RLRTLFPYINEASENHKKRVQSSTLNEVVTDAISMNPTPTDKGRRLNVFYATQVAIEP
                     PTFVVFVNDVELMHFSYKRYLENQIRAAFGFEGTPIHIIARKRN"
     gene            complement(3852..5027)
                     /gene="rpsA"
                     /locus_tag="NBH32_002400"
     CDS             complement(3852..5027)
                     /gene="rpsA"
                     /locus_tag="NBH32_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCD1515969.1"
                     /translation="MTEEFNESMINDIKEGDKVTGEVQQVEDKQVVVHINGGKFNGII
                     PISQLSTHHIDSPSEVVKEGDEVEAYVTKVEFDEENETGAYILSRRQLETEKSYSYLQ
                     EKLDNNEIIEAKVTEVVKGGLVVDVGQRGFVPASLISTDFIEDFSVFDGQTIHIKVEE
                     LDPENNRVILSRKAVEQEENDAKKDQLLQSLNEGDVIDGKVARLTQFGAFIDIGGVDG
                     LVHVSELSHEHVQTPEEVVSIGQDVKVKIKSIDRDTERISLSIKDTLPTPFENIKGQF
                     HENDVIEGVVVRLANFGAFVEIAPGVQGLVHISEIAHKHIGTPGEVLEPGQQVNVKIL
                     GIDEENERVSLSIKATLPNEDVVESDPSTTKAYLENEEEDNPTIGDMIGDKLKNLKL"
BASE COUNT         1602 a          990 c          682 g         1873 t
ORIGIN      
        1 atattgatac cgaaatgaga taacgtaata tatgggaaaa gtgtttcaat ttttactatt
       61 gcttgagaaa tttcataatc atttaaagct tgatgatgta atgaagattt caattcatta
      121 atttcaacaa ttgctttact aatttcattt tgaaatgata aatcattgat ttctgctagt
      181 agcgtataaa aatgcgcact aattaaatct ccaatcaaaa tggaatgttt agacaaatga
      241 ttatatgtaa tgtcatcaag gtgtctcatt gatgtgtcaa ttgtgaggca tgctactttg
      301 gcaacatctg gaatatcata tgaatcaagt aacttaccta accgatgatt aatattaatg
      361 gattcatatt ctgatacacc ctttaatctt tcttctattt gtctttccaa tttgctaaca
      421 gttgtttcca tgtctacacc tcacgatatt atcactattc atattaacat tatatgtaag
      481 aaattaaaat cttttgaagc attaagatta cttatcattt ttaaatttca atttaaacta
      541 acagtaattt atgtagcttt tgtaattctc ataataacct ttacttcatt tacattattc
      601 atctttttaa ataacgtaaa atactatagt agacatggtt accttttaag taaattcaac
      661 gctaacaatt aatctcatta ttcatatgta tattttgtag ttgctatttt tatcaaaact
      721 attatgtcat gtaatatttt gaacgcttaa acaatgaatg ttccaaagat taaaaactta
      781 gaagaacatt acaaattatg tacaggttgt aagtattaaa agcaaaataa aaagccctta
      841 ttcagggctt tttaagtaaa attattttac agcatctttt aatgctttac cagctttgaa
      901 tgctggaact ttacttgctg ggatatcaat ttctttacca gtttgagggt tacgaccttt
      961 acgtgcagca cgttcacgta cctcaaagtt accgaaacca attaattgta ctttttcacc
     1021 tttagcaagt gagttttgga ttgattcgaa tacagcatct actgctgaac cagcttcttt
     1081 tttagttaaa tcagcttgct ctgcaactgc attgattaaa tctgttttgt tcattagaca
     1141 ttcacctcct gaggtttgat aatgatatgt ttatatcatt atggtaaaac tttaacacaa
     1201 gcattacata aatcgcaatc atttatgtgg caaaaacgtt gaatttaagc catttctatt
     1261 cattttacgt aattattagc cgtatatgtg taataataca cattttattc agatttttta
     1321 tcgcgctcca ttaaatcttt tacgcattct tttactgaga tattttcaaa taatactcta
     1381 tataatgcat ttgtaattgg catatccaca tttttttctt tagctaaatg ataaactgat
     1441 ttagttgtat aaataccttc aacaaccata ttcatttcag ataatgcttg atccattgat
     1501 tcaccttgtc caagtttata tcctaatgtg aaatttcgag aatgtgttga tgtgcaagta
     1561 acgattaagt caccgatacc acctaaacct agaaatgtca taggatcggc acctaacttt
     1621 tcacctaatc tactaatttc cgctaaacca cgagtcatta acgcagcttt tgcattatca
     1681 ccgtagccaa ttccagctac gataccactt gctactgcga tgatattttt caatgcacca
     1741 ccaagttcaa caccaatcaa gtcatcattc gtgtacacac gcaaataatc attcataaat
     1801 aaatcttgcg ttaatttact tacactttta tcttttgatg atgcagcaac tgtagttggt
     1861 tgcttgacta caacttcttc cgcatgactt ggccctgaca acacgccaat acctgcatta
     1921 tattcgggtg aaatagaatc ttcaatcatt tctgacacac gtttaaacgt cccattttca
     1981 atacctttag caacatgtat aaaagtcttt ttagaggtca gcttatcatt aatttgagta
     2041 gcaacttctc gcattgcttt agtaggtaaa gccattaagt aaatatctgc aaattgaatt
     2101 gctttggtca tatctgaagt agcgatgatg ttaacatcta atttcgcgta ttttaaatac
     2161 tttttatttg tatgacatgt atttaattca tcaacagcat cttgattttt accccacatc
     2221 aaaacatcat gtccattttc tgcaagaaca ttggcaaggg ctgtcccaaa acttcccata
     2281 ccaaaaacgg taattttagt cattgttatc ccccaatcgt taatttctct ttcgagctat
     2341 aatatgaatt ggtgtacctt caaaaccaaa agcggcacgg atttgattct ctaaatagcg
     2401 tttataagaa aaatgcatta attctacatc attaacaaat acaacaaatg tcggtggttc
     2461 tatagcaact tgtgttgcat aaaagacatt caaacgtcta cctttgtctg ttggtgtagg
     2521 gttcatggaa attgcatcag taacgacttc atttaaagtt gaactttgaa cacgtttttt
     2581 atggttttca cttgcttcat taatgtaagg gaataatgta cgtaatcttg tgcgttcttt
     2641 agcagacaca aaagcaattt gtgcataatc taaaaattgg aattctttac gtacttcatc
     2701 ttcaaatttc ttcatcgttt tactatcttt ttccacagta tcccatttat ttacgacaat
     2761 cacgactgct ttaccttgtt catgtgcata tcctgcaacg cgtttatctt gttcaatgat
     2821 gccttgttct gcatcaatga ccactaaaac aacatttgaa cgttcaatcg cttttaaagc
     2881 tcttagtact gaatatttct cagttgattc atatactttt ccttttttac gcataccagc
     2941 agtatcgatt aaaacataat cttgtccatc ataactatac tctgtatcaa tagcgtctct
     3001 cgttgtccct gcaacattag aaacgataac gcgatcttca cctaaaatag catttactaa
     3061 acttgattta cctacgtttg gtcgtccaat aatggatagt cgaattgtat cttcatcata
     3121 aggatcttct tcctcttcac caaaatgaga aacaactgca tctaacaagt caccaagacc
     3181 taaaccatgt gaccctgata tcggatacgg ttcaccaaat cctaatgaat agaaatcata
     3241 cacgtctgta cgcatttcca tattatctac tttgttaacc gctaatacga ccggtttttt
     3301 agatttgtat aaaatttgag cgaccatttc atcgctttgt gtcaatcctt cacgcacgtt
     3361 aaccataaaa ataataacat ccgcttcatc tatggcgatt tctgcctgcg ctctaatttg
     3421 tgtttggaat ggtgcatcac caatttcaat accacctgta tcaataatat tgaaatcatg
     3481 tgttaaccat tcacctgaag aataaatacg atctcgtgtt acacctggcg tgtcttccac
     3541 aatcgaaaca cgttctccaa ctattctatt aaaaattgta gatttaccta cattaggcct
     3601 acctacaata gctactatag gtttagtcat aagctaactt cctctctttc ttatatcttt
     3661 ttaataatat cacatgagac tgtgtattca aaatgagttt attactgtaa tgataaaaat
     3721 cagcacttaa aactttatct ctttaaacac ttagttaaat ttgtccacgt tatcattccg
     3781 agtatatttt ggtacaaaaa aatactccac atgcaatcat aaacatgtgg agttgactat
     3841 taaatattaa attatagttt aagattttta agtttatcac caatcatatc gccaattgtt
     3901 ggattatctt cttcttcgtt ttctaagtac gccttagtcg tagaaggatc actttcaaca
     3961 acatcttcgt ttggtaatgt tgctttaata gatagtgata ctctttcatt ctcttcatca
     4021 atacctaata ttttaacatt tacttgttga ccaggttcta acacttcacc tggcgtacca
     4081 atgtgtttgt gtgcaatttc agaaatatgt acaagtcctt gtacacctgg tgcaatttct
     4141 acaaatgcac caaagtttgc caatcttact acgacacctt caatgacatc attttcgtgg
     4201 aattgacctt taatattttc gaaaggtgtt ggtaacgtat ctttgattga taatgaaata
     4261 cgttctgtat ctctatcaat agatttaatt ttaactttaa catcttgacc aattgaaact
     4321 acttcttctg gtgtttgaac atgttcgtga gaaagttcag atacatgcac taaaccatca
     4381 acaccgccaa tgtctataaa tgcaccaaat tgagttaaac gtgctacttt accatcaata
     4441 acatcgcctt catttaaaga ttgtaataat tgatcttttt tagcatcatt ttcttcttgt
     4501 tcaactgctt tacggcttaa aatgactcta ttattttcag gatccaattc ttcaacttta
     4561 atatgaattg tttgtccatc aaatacagag aaatcctcaa tgaagtctgt tgaaattagt
     4621 gaagctggaa caaaacctct ttgtcctaca tcaacaacca aaccaccttt aactacttct
     4681 gttactttcg cttcgatgat ttcattatta tctaattttt cttgtaaata actataagac
     4741 ttctcagttt caagttgtct tctagataag atgtaagctc cagtttcatt ttcttcatca
     4801 aactcaactt tagtgacata tgcttcaact tcgtcgccct cttttacaac ttcacttggg
     4861 ctatcaatat gatgcgtaga tagttgacta ataggaataa tcccattaaa tttaccaccg
     4921 ttgatatgaa caacaacttg cttgtcttca acttgctgta cctcgccagt gactttgtca
     4981 ccttctttaa tatcgttaat cattgattca ttgaattctt cagtcatctt gtttgcctcc
     5041 ttatacacta catatttata acatcttata aactgacaga attgtcaaga aattctacct
     5101 ttattacatt tagtaaatta gttgtatcca taaatatgtc acttaat
//
LOCUS       DAHHOJ010000138         5052 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.139, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000138 DAHHOJ010000000
VERSION     DAHHOJ010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5052
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.139"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(243..764)
                     /locus_tag="NBH32_002401"
     CDS             complement(243..764)
                     /locus_tag="NBH32_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwhD family protein"
                     /protein_id="HCD1515970.1"
                     /translation="MEVISLSEKKGFNFNIIKNDPLDGHKGTNIGSISLDNIAPVFID
                     VANKEAFIDIGGMHARAKVEKGVKWITDKAAVEGDEAKEYWLCWVTTERNEQGPYYAG
                     LTACYLLVNKAIRRGYKSMPEHVNMMDKSMKHHIIIDQIGDENKAILKDFLMNHDEGM
                     WKHSSDALHQAFN"
     gene            complement(792..2087)
                     /locus_tag="NBH32_002402"
     CDS             complement(792..2087)
                     /locus_tag="NBH32_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCD1515971.1"
                     /translation="MTNAYVDLKLVEEKVFKDPIHRYIHVEDQLIWDLIKTKEFQRLR
                     RIRQLGTLYLSFHTAEHSRFGHSLGVYEIVRRLIDESFIGHDAWDNKDRPLALCAALL
                     HDLGHGPFSHSFEKIFNTDHEAYTQAIITGDTEVNAVLRKVAPEFPREVAEVINKTHH
                     NKLVISMISSQIDADRMDYLQRDAYFTGVSYGAFDMERILRLMRPSKDEVLIKESGMH
                     AVENFIMSRYQMYWQIYFHPVSRGGEVLLNNCLKRAKQLYNEGYEFKLHPHDFIPFFE
                     ETVTIEQYVELDEAVVTYYLEKWTKEDDAILSDLASRFINRDLFKYIPFDGSIITISE
                     LQELFEAGGINPDYYFVSEAFSDLPYDYDRPGSNRKPIHLLRQDGTIREISNQSLVIH
                     SITGINRQDYKLYYPREMVAKIKDKTIREAIENLINELN"
     gene            complement(2127..2603)
                     /locus_tag="NBH32_002403"
     CDS             complement(2127..2603)
                     /locus_tag="NBH32_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCD1515972.1"
                     /translation="MFKVRQATEKDVVQIRDVATKAWFNTYLNIYAATTVNHLLEASY
                     NEHHLKKRLQEQLFLVVEEGNDIVGFANFIYGEELYLSAHYVKPESQHTGYGTALLNE
                     GLSRFEDKFEGVYLEVDNKNEEAVAYYKEQGFTILRSYEPEMYGEKLDLALMYKAF"
     gene            complement(2772..3308)
                     /locus_tag="NBH32_002404"
     CDS             complement(2772..3308)
                     /locus_tag="NBH32_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin family protein"
                     /protein_id="HCD1515973.1"
                     /translation="MITVLFGGSRPNGNTAQLTKFALQDLEYQWIDVTQHQFKPIRDV
                     RHTAETITSYDDDYLPILDKILASDTIIFASPVYWYSISAPLKAFIEHWSETLQDKRY
                     PNFKAQMAEKDFRVILVGGDCPKIKAKPAITQMKYSLDFLGATLNGYIIGTAEKPGDI
                     MKDNYALARATEWNSILQ"
     gene            complement(3748..4686)
                     /locus_tag="NBH32_002405"
     CDS             complement(3748..4686)
                     /locus_tag="NBH32_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCD1515974.1"
                     /translation="MKQFVNLGKSDVEVFPIALGTNAVGGHNLYPNLDEEQGKDVVRQ
                     AINHGINLLDTAYIYGPERSEELVGEVVKEYPREQIKIATKGSHEFDENQEVHQNNQP
                     EYLKQQVENSLKRLQTDYIDLYYIHFPDDNTPKDQAVAALQELKEQGKIKAIGVSNFT
                     LDQLKEANKDGYVDVVQLEYNLLHRENEAVLQYCVDHQITFIPYFPLASGILAGKYDE
                     NTKFSDHRTTRRDFKPGVFEENVRRVKALESIAAAHQTSIANIVLAFYLTRPAIDVII
                     PGAKLAEQVVENIKAADIVLSDDEIQYIDELFPIED"
BASE COUNT         1605 a          954 c          681 g         1812 t
ORIGIN      
        1 tttataatac tcttatcttc actcttatta taatgtgaat taattcacaa agtatatagc
       61 tatgatcaaa aaaatgtgtc agttattcaa attttttatt aactacagat taatttaatt
      121 tcaaaaaaat taaaaaaact cccaatcaat ctaagacttt attttcacct caagatttac
      181 gtcgtcattg agaaatgaat catatcttta tagattaatt gggaaatttt agtttctaat
      241 atttaattaa atgcttgatg taaagcatca gaagaatgct tccacatacc ttcatcatgg
      301 ttcattaaaa agtcttttaa aatagcttta ttctcgtcac caatttgatc aatgataata
      361 tgatgtttca ttgatttatc catcatatta acatgttcag gcatactttt ataaccacga
      421 cgaattgctt tattcactaa taaatagcac gctgttaaac cagcataata tggtccttgt
      481 tcattacgtt ctgttgttac ccaacacaac caatattctt tagcttcatc gccttcaaca
      541 gcagctttat cagtaatcca tttcacgcct ttttcaactt tggcgcgagc atgcatgcct
      601 ccaatatcaa taaatgcttc tttgttagca acatcgataa aaactggtgc aatattgtct
      661 aagctaattg aaccaatatt tgtaccttta tgaccatcta gagggtcatt ttttatgata
      721 ttaaaattaa agcctttttt ctcagacaat gatataacct ccatatttaa cgataacaat
      781 tttagccctt tttaattaag ctcattaatc aaattttcaa tagcttctct aattgtctta
      841 tctttaatct ttgcaaccat ttctctagga taatataatt tatagtcttg gcgattaatg
      901 cctgtaatac tatgaatgac taaagattga ttgcttattt ctctaatcgt accatcttgt
      961 cttaataaat gaatcggttt gcgatttgac ccaggtcgat catagtcata tggcaaatcc
     1021 gaaaatgctt cactcacaaa ataataatct ggattaatac cacctgcttc aaacagttct
     1081 tgcagttctg atattgtaat aattgagcca tcaaatggaa tatatttaaa taagtctcga
     1141 ttaataaatc gacttgctaa atcacttaaa atagcatcat cttcttttgt ccatttttcc
     1201 aaataatacg taactaccgc ttcatcgagt tcaacatatt gttcaatcgt aactgtctct
     1261 tcaaaaaatg gaataaaatc atgtggatgc aacttaaatt catagccttc attataaagc
     1321 tgttttgcgc gtttcaaaca attattaagc agcacttctc caccacgact aactgggtgg
     1381 aaataaattt gccaatacat ttgataacga ctcataataa agttttcaac tgcatgcata
     1441 ccactttctt taattagtac ttcatcttta gaaggtcgca ttaatcttaa aatacgttcc
     1501 atatcaaaag caccatatga tacacctgtg aaatacgcat cacgttgtaa ataatccatt
     1561 ctatccgcat cgatttgtga agaaatcatc gaaatgacca atttattatg atgcgtttta
     1621 ttaattactt ccgcaacttc tcttggaaac tcaggcgcca ctttacgtaa tacagcattc
     1681 acctcagtat ctccagtaat aatcgcttgt gtgtatgctt catggtctgt attaaatatt
     1741 ttttcaaaac tatgtgaaaa tggaccatgt cctaaatcat gtaataatgc agcacataat
     1801 gccaacggtc tatctttatt gtcccatgca tcatgaccaa taaatgactc atcaattaat
     1861 cgtctaacta tttcatacac acctaaagaa tgtccaaagc gactatgttc tgctgtgtga
     1921 aaagataggt acagtgttcc tagttgtcta attcgacgta acctttggaa ttccttagtt
     1981 ttaattaaat cccatatcaa ttgatcttca acatgaatat atcgatgtat cgggtcttta
     2041 aaaacttttt cttctactaa ttttaaatct acatatgcgt tagtcattat tcccctcctt
     2101 ttcgtttaat ataatattta atttacttaa aatgctttgt acataagtgc taagtctaac
     2161 ttttcgccat acatttctgg ctcataagag cgtaagattg taaaaccttg ctctttatag
     2221 taagctactg cttcttcatt tttattatct acttctaagt aaacaccttc aaatttatct
     2281 tcaaaacgtg ataatccttc atttaacaat gctgtaccat aacctgtatg ttgcgattct
     2341 ggtttaacat aatgagctga taaatataat tcttcaccgt aaataaagtt agcaaagcca
     2401 acgatgtcat taccttcttc aacgactaag aataattgtt cttgaagtct tttctttaaa
     2461 tgatgttcat tatatgaagc ttctaacaag tgattaactg ttgtcgcagc gtatatattt
     2521 aagtatgtat taaaccaagc tttagttgcg acatctctaa tttgaacaac atctttttca
     2581 gttgcttgtc ttaccttgaa catgactttc tccccttatt aacaagtttt aataacggca
     2641 ttataccaca acttgctcaa tacttaataa acaatgattg tctattcaat ttatatattt
     2701 atattttccg ttaaaattaa aaataaaaaa taacgaagca aaaaatcact tcgtttagta
     2761 tgaggtatgt cttattgcaa tatactattc cactcagttg cacgtgctaa ggcatagttg
     2821 tctttcatga tgtcaccagg cttttcagca gttccaataa tataaccatt taaagtggca
     2881 cctaaaaagt ctaaactata tttcatttgc gtaattgctg gcttcgcttt tatttttgga
     2941 caatctccac caactaaaat aactctaaaa tccttttcgg ccatttgtgc cttaaaatta
     3001 ggatatcgtt tatcttgtaa tgtttctgac caatgttcga taaatgcttt caatggtgct
     3061 gaaatgctat accaatacac tggtgatgca aaaataattg tatcactagc caatatttta
     3121 tctagaatcg gcaaatagtc atcgtcatat gaagtaatag tctctgctgt atgtctcacg
     3181 tcacgtatcg gtttaaactg atgttgtgtc acgtcaatcc attgatactc taaatcttgc
     3241 aaagcgaatt ttgttaattg tgcagtatta ccgtttggtc tactcccacc aaacaaaaca
     3301 gtaatcattt tagcctaacc tcacttttga ttaataaata tctgtgtttt tcgttaccta
     3361 attatactat cataagcttt gcctaccgaa tagtaaaacg cttacaactt ttatataaat
     3421 ttgacgaaat ttcgtcatcc ttatataacg tcgtttgtga tgggagtggg acagaaatga
     3481 tattttcgca aaatttattt cgtcgtcccc cccaacttgc acattattgt aagctgtctt
     3541 ttcgccagct tctatgttgg ggcccgccaa cttacattgc ctgtagaatt tctttttgaa
     3601 attctctgtg ttggggcccc tgactaggat tgaaaaaagc ttgttacaag cgcattttcg
     3661 ttcagtcaac tactgccaat ataacttcgt agagcataga acattgattt atgtcccagc
     3721 ctgatatcac cattaaatac aattcattta gtcttcaatc ggaaacagtt catcgatata
     3781 ttgaatctca tcatctgata aaacgatatc tgcagcttta atattttcaa cgacttgttc
     3841 tgcaagtttt gcaccaggaa taatcacatc gatagctggt ctcgttaaat aaaatgctaa
     3901 tacaatgttc gcaattgaag tttgatgtgc tgcagctatg ctttccaaag cttttacgcg
     3961 acgcacattt tcttcaaata cacctggttt aaaatcacga cgtgtagtac gatggtcact
     4021 aaatttagtg ttctcatcat attttccagc taaaataccg gatgctaatg ggaaatatgg
     4081 aataaatgtg atttggtgat caacacaata ttgtaatact gcctcatttt cgcgatgcaa
     4141 taaattatat tctaactgta caacatcaac gtaaccatct ttatttgctt ctttaagttg
     4201 atctaatgtg aaatttgata caccaatggc tttaatcttc ccttgttcct taagctcttg
     4261 taatgctgca actgcttgat ctttcggagt gttgtcatcc ggaaaatgaa tataatataa
     4321 atcgatataa tcagtttgta gacgtttcaa actattctca acttgttgtt ttaaatattc
     4381 aggttgattg ttctgatgta cttcttgatt ttcatcaaat tcatgagacc cttttgtagc
     4441 aattttaatt tgctctcgcg gatattcttt aacaacttct ccaaccaatt cttctgatcg
     4501 ttctggccca taaatatatg ccgtatctaa taaattaata ccatgattaa tggcttgacg
     4561 aacaacatct tttccttgtt cttcatctaa gttcggatat aaattatgcc cacctactgc
     4621 gttcgtccca agtgcgattg gaaacacttc aacatcagat ttacctaagt ttacaaattg
     4681 cttcattaga cccagctcct tatctttata acttttccac tatcacacta ccatgattta
     4741 atacaacttc caaatgttag aattagagct tgaatttaag cggtgtaata ggaaaacgtt
     4801 atataaaact cagttaaaat aagtattgct taaattgata tgcatatagc gcgattgctc
     4861 gttgaccttt aaattacatg tacttttgaa tcagaaattg aaaaacattt aagaacgaca
     4921 acattaaagc cattacaaat ttaattagaa atactataat gttttgattt gcataacaaa
     4981 gaaattaaaa ctgttaatat taacatggga tatccattat aaaatattca tctttgtagt
     5041 tgataattct at
//
LOCUS       DAHHOJ010000139         4977 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.140, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000139 DAHHOJ010000000
VERSION     DAHHOJ010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4977
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.140"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(71..1828)
                     /gene="pyk"
                     /locus_tag="NBH32_002406"
     CDS             complement(71..1828)
                     /gene="pyk"
                     /locus_tag="NBH32_002406"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCD1515975.1"
                     /translation="MRKTKIVCTIGPASESEEMIEKLINAGMNVARLNFSHGSHEEHK
                     GRIDTIRKVAKRLDKIVAILLDTKGPEIRTHNMKDGIIELERGNEVIVSMNEVEGTPE
                     KFSVTYENLINDVQVGSYILLDDGLIELQVKDIDHAKKEVKCDILNSGELKNKKGVNL
                     PGVRVSLPGITEKDAEDIRFGIKENVDFIAASFVRRPSDVLEIREILEEQKANISVFP
                     KIENQEGIDNIAEILEVSDGLMVARGDMGVEIPPEKVPMVQKNLIRQCNKLGKPVITA
                     TQMLDSMQRNPRATRAEASDVANAIYDGTDAVMLSGETAAGLYPEEAVKTMRNIAVSA
                     EAAQDYKKLLSDRTKLVETSLVNAIGISVAHTALNLNVKAIVAATESGSTARTISKYR
                     PHSDIIAVTPSEETARQCSIVWGVQPVVKKGRKSTDALLNNAVATAVETGRVTNGDLI
                     IITAGVPTGETGTTNMMKIHLVGDEIANGQGIGRGSVVGTTLVAETVKDLEGKDLSDK
                     VIVTNSIDETFVPYVEKALGLITEENGITSPSAIVGLEKGIPTVVGVEKAVKNISNNM
                     LVTIDAAQGKIFEGYANVL"
     gene            complement(1850..2818)
                     /gene="pfkA"
                     /locus_tag="NBH32_002407"
     CDS             complement(1850..2818)
                     /gene="pfkA"
                     /locus_tag="NBH32_002407"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002462395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCD1515976.1"
                     /translation="MKKIAVLTSGGDSPGMNAAVRAVVRTAIYNEIEVYGVYHGYQGL
                     LNDDIHKLELGSVGDTIQRGGTFLYSARCPEFKEQEVRKVAIENLRKRGIEGLVVIGG
                     DGSYRGAQRISEECKEIQTIGIPGTIDNDINGTDFTIGFDTALNTIIGLVDKIRDTAS
                     SHARTFIIEAMGRDCGDLALWAGLSVGAETIVVPEVKTDIKEIADKIEQGIKRGKKHS
                     IVLVAEGCMTAQDCQKELSQYINVDNRVSVLGHVQRGGSPTGADRVLASRLGGYAVDL
                     LMQGETAKGVGIKNNKIVATSFDEIFDGKDHKFDYSLYELANKLSI"
     gene            complement(3087..4031)
                     /locus_tag="NBH32_002408"
     CDS             complement(3087..4031)
                     /locus_tag="NBH32_002408"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit alpha"
                     /protein_id="HCD1515977.1"
                     /translation="MLDFEKPLFEIRNKIESLKESQDKNDVDLQEEIDMLEASLERET
                     KKIYTNLKPWDRVQIARLQERPTTLDYIPYIFDSFMELHGDRNFRDDPAMIGGIGFLN
                     GRAVTVIGQQRGKDTKDNIYRNFGMSHPEGYRKALRLMKQAEKFNRPIFTFIDTKGAY
                     PGKAAEERGQSESIATNLIEMASLKVPVIAIVIGEGGSGGALGIGIANKVLMLENSTY
                     SVISPEGAAALLWKDSNLAKIAAETMKITAHDIKQLGIIDDVISEPLGGAHKDIEQQA
                     LAIKSAFVEQLDSLESLSRDEIANDRFEKFRNIGSYIE"
     gene            complement(4031..4888)
                     /locus_tag="NBH32_002409"
     CDS             complement(4031..4888)
                     /locus_tag="NBH32_002409"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HCD1515978.1"
                     /translation="MFKDFFNRTKKKKYLTVQDSKNNDVPAGIMTKCPKCKKIMYTKE
                     LAENLNVCFNCDHHIALTAYKRIEAISDEGSFTEFDKGMTSANPLDFPSYLEKIEKDQ
                     QKTGLKEAVVTGTAQLDGMKFGVAVMDSRFRMGSMGSVIGEKICRIIDYCTENRLPFI
                     LFSASGGARMQEGIISLMQMGKTSVSLKRHSDAGLLYISYLTHPTTGGVSASFASVGD
                     INLSEPKALIGFAGRRVIEQTINEKLPDDFQTAEFLLEHGQLDKVVHRNDMRQTLSEI
                     LKIHQEVTK"
BASE COUNT         1528 a         1029 c          681 g         1739 t
ORIGIN      
        1 taaaatacca gataaaccaa aaaaacgtct ttccatagtt gataaatgga aagacgtctt
       61 tatttataaa ttatagtacg tttgcatatc cttcaaagat tttaccttga gcagcatcaa
      121 tcgtaactaa catgttattg cttatgtttt taacagcttt ttctacacct acaactgttg
      181 gaataccttt ttctaaacca acaattgcac ttggtgatgt aataccattt tcttctgtaa
      241 ttaagcctaa agctttttct acataaggta caaaagtttc atcgattgag ttagtaacga
      301 taactttgtc agataaatct ttaccttcta aatctttaac agtttcagca actaacgtag
      361 ttccaacaac tgatccacgt ccaatacctt gaccattagc aatttcgtca ccaactaggt
      421 ggattttcat catattagta gttccagttt caccagttgg tacaccagca gtaataatga
      481 ttaaatcacc attagttact ctaccagttt caacagctgt tgcaactgca ttgtttaaca
      541 atgcatctgt actcttacgt ccttttttaa ctacaggttg aacaccccaa acaattgaac
      601 attgacgtgc agtttcttca cttggagtca ctgcaataat gtctgaatgc ggacgatatt
      661 tagagatagt acgtgccgtt gaaccacttt cagtagcagc tacaattgct ttaacattta
      721 agtttaaagc tgtatgtgca accgaaatac cgattgcatt cactaatgaa gtttcaacta
      781 atttagtacg atctgacaat aactttttgt aatcttgggc tgcttcagct gatacagcaa
      841 tatttctcat tgttttaaca gcttcttcag gatataaacc agcagcagtt tcaccagata
      901 acattactgc atctgtacca tcatagattg cgttggcaac gtcactagct tctgcacgtg
      961 tagcacgtgg gttacgttgc atagaatcta acatttgtgt agctgtaata actggtttac
     1021 ctaatttgtt acattgtctg attaaatttt tttgaaccat tggtactttt tcaggtggaa
     1081 tttcaacacc catgtcacca cgtgcaacca ttaaaccatc agacacttca agaatttccg
     1141 caatattatc aataccttct tggttttcaa ttttagggaa tactgaaatg ttagcttttt
     1201 gttcttctaa aatttcacga atttctaaaa catcactagg acgacgtacg aaacttgctg
     1261 caatgaagtc aacattttct ttaataccga aacggatatc ttcagcatct ttttctgtaa
     1321 taccaggtaa acttactctt acaccaggta agttaacacc ttttttgttt ttaagctcac
     1381 cagagtttaa aatatcacat ttaacttctt ttttagcatg gtcaatatct ttaacttgta
     1441 attcaattaa gccatcatca agtaaaatgt atgaacctac ttgaacatcg ttaattaagt
     1501 tttcatatgt tactgagaac ttttccggtg ttccttcaac ttcattcatg ctaacaataa
     1561 cttcgttacc acgttcaagt tcaatgatac cgtctttcat attatgcgta cgaatttctg
     1621 gacctttcgt atctaataaa attgctacaa ttttgtctaa tcttttagct actttacgaa
     1681 ttgtatcaat tctaccttta tgctcttcat gactaccatg tgaaaagttt aatcgtgcaa
     1741 cgttcatacc agcattgatt aatttctcaa tcatttcttc tgattctgaa gctggtccaa
     1801 ttgtacatac aattttagtt tttctcattt tataattcct cctgaaatct tatatagata
     1861 acttgttagc aagttcatat agactataat caaatttatg atctttacca tcaaaaattt
     1921 catcaaaaga tgttgctaca attttattgt tcttaattcc aacaccctta gctgtttcac
     1981 cttgcattaa taagtctacc gcatatccac ctaaacgtga tgctaaaact ctatccgcac
     2041 ctgttgggct accaccacgt tgaacgtgac ctaacacaga cactctatta tcaacattga
     2101 tgtattgtga taattctttt tgacaatctt gcgcagtcat gcaaccttct gctacaagaa
     2161 cgattgagtg tttcttacca cgtttaatac cttgttcaat tttatcagct atttctttaa
     2221 tatctgtttt cacttctggt actacaattg tctcagcacc aactgataat ccagcccata
     2281 atgctagatc tccacaatca cggcccattg cttcaatgat aaatgttcgt gcgtgacttg
     2341 acgcagtatc tctaattttg tcgactaagc caataatcgt atttaatgct gtgtcaaatc
     2401 caattgtaaa atcagtacca ttgatatcat tgtcaatcgt accaggaata ccgatagttt
     2461 gaatttcttt acattcctca ctgatgcgtt gtgcaccgcg ataactaccg tcaccaccaa
     2521 taactacaag gccctcaatc cctcttttac gtaagttttc gattgcaact ttacgtactt
     2581 cttgctcctt aaactctgga catcttgctg aatacaagaa tgtacctcca cgctgaatcg
     2641 tatccccaac tgatcctaat tcaagtttat gaatatcatc atttaacaat ccttggtaac
     2701 catgatacac accataaact tcaatttcat tgtaaattgc tgtacgaaca actgctctta
     2761 ccgcagcatt cattccaggt gaatctccac cactagttaa aactgcaatt ttcttcatga
     2821 cgacatacct ttctataact gatttatctt taactctaaa ttaccacaaa ttgatatgtc
     2881 attccatcaa aactaatgta ttgaattgat gtaaacgttt taaatattga attttatttt
     2941 aacttaatta aaaaaagatt agtagccaca attttgacta ctaatcttag ttaaaattca
     3001 gtatctaaat attaaattat tgttctaatg tacttcaatt tatggtaaaa cccagtatcg
     3061 atttaacata aaaatgctca agttgattat tctatataag aaccgatatt tctgaatttt
     3121 tcaaaccgat cattagcaat ttcatcacgt gataatgact caagtgaatc taactgttca
     3181 acaaacgctg atttaatagc taaagcttgc tgttcaatat ctttatgtgc accgccaagt
     3241 ggttcagaaa tgacatcatc tataatacct aattgcttaa tatcatgggc agtaattttc
     3301 attgtttcag ctgcaatttt agccaaatta ctgtctttcc ataataatgc cgctgcacct
     3361 tcaggagata taacagagta agtactattc tctaacatca atactttatt ggcaatacca
     3421 atacctagag cacctccact gccaccttca ccaatgacaa tcgcaataac tggtactttt
     3481 agtgaagcca tctcaatcaa atttgttgcg atagattcac tttgtccacg ttcttccgca
     3541 gctttaccag gatatgcacc ttttgtatct ataaatgtaa agataggacg attgaacttt
     3601 tcagcttgtt tcattaaacg taatgctttt cgataacctt ctggatgcga cataccaaaa
     3661 tttcgataaa tattatcttt tgtatctttt ccgcgttgtt gtccaataac cgtaacagcg
     3721 cgaccattta aaaagccaat gccaccaatc attgctggat catctctaaa attacgatca
     3781 ccatgaagtt ccataaacga atcaaagata tatggaatat aatctagggt cgtaggtctt
     3841 tcttgcaaac gcgcaatttg cacacgatcc catggtttta gatttgtata tattttttta
     3901 gtttctcgtt ccaatgacgc ttcaagcatg tcaatttctt cttgtaaatc cacatcattt
     3961 ttatcttgag attctttcaa agattcaatt ttatttcgaa tttcaaaaag tggtttttca
     4021 aaatctaaca ttatttagtc acctcttgat ggatttttag aatttcagac aatgtttgac
     4081 gcatatcatt acgatgtaca actttgtcca attgtccatg ctctaataaa aattctgcag
     4141 tttggaaatc atctggcaat ttttcgttta ttgtctgttc aataactcga cgacctgcaa
     4201 aacctatcaa cgcttttggc tcacttaaat ttatatcacc aactgatgca aagcttgcag
     4261 atacaccacc agtagttgga tgtgttaaat atgatatata taatagtcca gcgtcagaat
     4321 gacgttttaa agatacactg gttttaccca tttgcatcaa ggaaataata ccttcttgca
     4381 tacgtgcacc accacttgca gagaaaagaa taaatggtaa acggttctca gtgcagtaat
     4441 caatgatgcg acatatcttt tcaccgataa ccgatcccat acttcccatt ctaaaacgtg
     4501 aatccatgac agcaacgcca aatttcatac catctagttg cgctgtacca gtcacaactg
     4561 cttctttaag acctgtcttt tgttggtcct tttcaatttt ttctaaataa cttggaaaat
     4621 ctaatggatt cgcagaggtc attcccttat cgaattctgt aaatgatcct tcatcagaaa
     4681 ttgcttctat acgtttatac gcagttaaag caatatgatg atcacaatta aagcacacat
     4741 ttaaattttc agctaattct tttgtgtaca taattttctt acactttgga cacttagtca
     4801 taatacctgc aggcacatca ttatttttag agtcttgtac tgtaagatat ttctttttct
     4861 ttgttcgatt aaaaaaatct ttaaacatag gaaaacctcc aaataacctc tgttctaacc
     4921 atttagagcc ttaaactgaa cttgaccagt taaaaacggg tttaaattta tttgatt
//
LOCUS       DAHHOJ010000140         4974 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.141, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000140 DAHHOJ010000000
VERSION     DAHHOJ010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4974)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.141"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            44..322
                     /locus_tag="NBH32_002410"
     CDS             44..322
                     /locus_tag="NBH32_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04197 family type VII secretion effector"
                     /protein_id="HCD1515979.1"
                     /translation="MMSTVQSDIFKTNSASSSIKSAVETCNNVSKPDKDESTTVSGNN
                     NAHSVIDDLMSKNQSVAEAIRTASDNIQKVGEAFDQTDVMIGNEIGKN"
     gene            337..705
                     /locus_tag="NBH32_002411"
     CDS             337..705
                     /locus_tag="NBH32_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515980.1"
                     /translation="MSNKLDEINKIITAKHEQMDDLYDEKREVKALIDESDALNHSID
                     QLYQHLGERYYSSNMASRMEQFRDEFHFAKRRSTVALHEQQQQIQHGIRKAEEEMIDL
                     EMRRNVEIETVTKEENKWKQ"
     gene            693..989
                     /locus_tag="NBH32_002412"
     CDS             693..989
                     /locus_tag="NBH32_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4176 domain-containing protein"
                     /protein_id="HCD1515981.1"
                     /translation="METIGSIIYLKEGSQKLMIINRGPIVEIENQKYMFDYSGCKYPI
                     GVVEDEIYYFNEENIDSVIFKGYSDQDEVRFQELFDNMKQNLDSEIQRGKVTQQ"
     gene            1324..2430
                     /locus_tag="NBH32_002413"
     CDS             1324..2430
                     /locus_tag="NBH32_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HCD1515982.1"
                     /translation="MQHHKVAIIGAGAAGIGMAITLKDFGITDVIILEKGTVGHSFKH
                     WPKSTRTITPSFTSNGFGMPDMNAISMDTSPAFTFNEEHISGETYAEYLQVVANHYEL
                     NIFENTVVTNISVDDAYYTIATTTETYHADYIFVATGDYNFPKKPFKYGIHYSEIEDF
                     DNFNKGQYVVIGGNESGFDAAYQLAKNGSDIALYTSTTGLNDPDADPSVRLSPYTRQR
                     LGNVIKQGARIEMNVHYTVKDIDFNNGQYHISFDSGQSVLTPHEPILATGFDATKNPI
                     VQQLFATTNQDIKLTTHDESTRYPNIFMIGATVENDNAKLCYIYKFRARFAVLAHLLT
                     QREGLPAKQDVIESYQKNQMYLDDYSCCEVSCTC"
     gene            2424..3764
                     /locus_tag="NBH32_002414"
     CDS             2424..3764
                     /locus_tag="NBH32_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000895187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HCD1515983.1"
                     /translation="MLEVKYDMRTGHYTPIPNEPHYLVISHADKLNATEKAKLRLLII
                     KQKLDISLAESVVSSPIASEHVIEQLTLFQHERRHLRPKISATFLAWLLIFLMFALPI
                     GIAYQFSDWFQNQYVSAWIEYLTQTTLLNHDILQHILFGDYGVLSLGTYSLVWALPVV
                     ILISLSTAIIDQTGFKSWMIWAIEPSMLRIGLQGNDIVPLLEGFGCNAAAISQAAHQC
                     HTCTKTQCMSLISFGSSCSYQIGATLSIFSVAGKSWLFMPYLILVLLGGILHNRIWLK
                     KNNQQLSVPLPYDRQLHMPNIRQMLLQMWQNIQMFIVQALPIFITICLIVSILSLTPI
                     LNVLSQIFTPILSLLGISSELSPGILFSMIRKDGMLLFNLHQGALLQVMTATQLLLLV
                     FFSSTFTACSVTMTMLLKHLGGQSALKLIGKQMLTSLSLVIGVGIIVKIVMLII"
     gene            3864..4754
                     /locus_tag="NBH32_002415"
     CDS             3864..4754
                     /locus_tag="NBH32_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HCD1515984.1"
                     /translation="MKIVIIGGFLGGGKTTVLNHLLAESLKESLKPAVIMNEFGKMSV
                     DGALVSEDIPLSELTEGCICCAMKADVSEQLHQLYLKEQPDIVFIECSGIAEPVSVLD
                     ACLTPILAPFTTITHMIGVVDASMYKHIKSFPKDIQGLFYEQLAYCSVLFVNKIDSAD
                     VETTSKLLKDLEVINPEADIQVGMHGSVTLPISVRQMTVTSDNKHESLHKTINHQFVQ
                     SPVKCTKAEFIKRLACLPSHIYRLKGFMTFEDTAHTYLIQFTQGQYELTPVAFSKKVP
                     EYLVLIGKGISKEDYQCLEQ"
BASE COUNT         1754 a          722 c          939 g         1559 t
ORIGIN      
        1 gtttctaata attttaaaat aatttaaatg ggaagaggtg taaatgatga gtacagttca
       61 aagtgatatt tttaagacca atagtgcatc atcatctatt aaaagcgctg ttgaaacatg
      121 taataatgtg tcgaaaccgg ataaagatga aagtacaaca gtaagtggaa ataataatgc
      181 tcatagtgtg atagatgatt tgatgagtaa gaatcaatct gttgctgaag caatacgaac
      241 tgcgagcgat aatatacaaa aagttggtga ggcttttgac caaactgacg taatgattgg
      301 taatgaaatt ggtaaaaatt aaaacgtggt gaaatgatgt cgaataaact ggatgaaatc
      361 aataaaataa tcacagcgaa acatgagcaa atggatgact tatatgatga aaagcgagag
      421 gttaaagcat tgatagatga aagtgatgcg cttaatcatt cgatagatca attatatcaa
      481 catttaggtg agcgttatta tagtagcaat atggctagtc gtatggaaca gttccgcgat
      541 gaatttcatt ttgcgaaacg acgttcaacg gtagcgttac acgagcagca acagcaaatt
      601 caacatggca ttcgtaaagc ggaagaagag atgattgact tggaaatgcg aaggaatgtt
      661 gaaattgaga cggtgacaaa ggaggaaaat aaatggaaac aataggaagc attatttatt
      721 taaaagaagg ttcgcaaaag ttaatgatta ttaatagagg accaattgta gaaattgaaa
      781 atcaaaagta tatgtttgac tattctggat gtaaatatcc gattggtgtt gtggaagatg
      841 aaatttatta ttttaacgag gaaaatatag attcagttat ttttaaaggt tattctgatc
      901 aagatgaggt tagatttcaa gagttgtttg ataatatgaa acaaaatttg gatagtgaaa
      961 tacaacgtgg aaaagttaca caacaataaa gaaatacttt ttctttattg gggtgggacg
     1021 acgaaataaa ttttgtaaaa atatgatttc tgtctcaccg ccaaaatgtt atatccaagt
     1081 actgaggttg gatgatacca ttaaatgtca ttcataatca tttgaactaa ccgtagcatc
     1141 tttaaattta ataaaaaaga cacataccaa cttccgaaat gtagatgaat tctctacaat
     1201 aacggaagtt tttcttttaa tattgaaatt tctcaaggat aggtctatac tttataaatc
     1261 gtaattatta cgatttatag tcaggaacaa taacttgaaa tagatcattg agggagtgtt
     1321 aatatgcaac atcataaagt ggctattatc ggtgccggtg ctgcaggtat aggtatggcc
     1381 attaccttaa aagatttcgg tataacagat gtcattattt tagaaaaagg aacagtaggc
     1441 cattcattta aacattggcc gaaatcgacc cgtacgatta cgccatcatt tacgtctaat
     1501 ggatttggca tgcctgatat gaatgcaatt tctatggata cttcaccagc atttacattt
     1561 aatgaagaac atatttccgg agaaacatat gctgaatatt tacaagtggt tgccaaccat
     1621 tacgagctga atatctttga aaatacagtt gttacaaata tatctgtaga tgatgcatat
     1681 tatacgattg caacgacaac agagacatat cacgcggatt atatctttgt cgcaacaggt
     1741 gattataatt tccctaaaaa gccatttaaa tatggtattc attatagtga aattgaagac
     1801 tttgataact ttaataaggg gcaatatgtg gttatcggag gtaatgaaag tggctttgat
     1861 gctgcatatc aacttgcaaa aaatggctct gacatcgcac tttatactag cacaaccggt
     1921 ttaaatgatc cggatgctga tcctagtgtt agattgtcac cttatacacg tcagcgacta
     1981 ggtaatgtca ttaaacaagg tgctcgtatt gaaatgaatg tacattatac agttaaagat
     2041 attgatttta acaacggaca gtatcatatc agttttgata gcggacaaag tgtgcttacg
     2101 cctcatgaac caatactagc aactggcttt gatgcaacaa aaaatccaat cgttcaacaa
     2161 ttatttgcga caacaaatca agatattaaa ttaacaacac atgatgaatc gacccgttat
     2221 ccgaatattt ttatgattgg tgcaacagtt gaaaatgata atgccaaatt atgctatatc
     2281 tataaattta gagcgcgatt tgcagtactt gcacatcttt taacacagcg ggaaggctta
     2341 ccagctaaac aagatgtcat tgaaagttac caaaaaaatc aaatgtattt agatgattat
     2401 tcatgttgtg aagtgtcatg cacatgttag aagtgaaata tgatatgaga actgggcatt
     2461 atacgcccat acctaatgaa cctcattatt tggttattag tcatgcggat aaacttaacg
     2521 caacagaaaa agcgaaatta agattattaa tcataaaaca gaaattagat atttcattgg
     2581 cagaaagtgt agtttcttcg cctatagcga gcgaacatgt gatagaacaa ttgacactat
     2641 ttcaacatga gcgacgacat ttaagaccta aaataagtgc gacattttta gcctggttgt
     2701 tgatattttt aatgtttgca ttgccaattg gtattgctta tcaattttca gattggtttc
     2761 aaaatcagta tgtatcagca tggatagaat atttaactca aacaacattg ctcaatcacg
     2821 atatattaca gcatatatta tttggtgatt atggtgtgct atcacttgga acatattcgc
     2881 tcgtatgggc attgccggtt gtaatattga ttagtttatc aactgctata attgatcaaa
     2941 caggattcaa atcatggatg atatgggcaa ttgaaccgtc aatgttacgg ataggattac
     3001 aaggcaatga tatcgtgcca ctattagaag gatttggatg taatgcagcc gccatttcac
     3061 aagcagcaca ccaatgccat acctgcacga agacacagtg tatgagttta ataagctttg
     3121 gtagttcttg tagttatcaa ataggtgcga cattatctat ttttagtgta gctggaaagt
     3181 catggctatt tatgccgtac ttaatattag tacttttagg tggcatctta cataatagga
     3241 tatggttgaa aaagaataat caacaactta gcgttccgct accttatgat agacaattac
     3301 atatgccaaa tatacgtcaa atgttgctac aaatgtggca aaatatacaa atgtttatcg
     3361 ttcaagcgct acctattttt atcacaatct gtcttattgt tagtatttta tcactaacgc
     3421 caattttgaa tgttttatca caaatattta cacctatatt atcgttatta ggcatctcgt
     3481 cagaattgtc accagggatt ttattttcaa tgattcgaaa agacggcatg ctcttgttta
     3541 atttgcatca aggcgcctta ttacaagtaa tgacagcaac acagttacta ctacttgtgt
     3601 tttttagttc aacatttaca gcgtgctcgg tcacaatgac gatgcttttg aaacatttag
     3661 gtggtcagtc agcactaaaa ttaattggaa agcaaatgtt gacatcattg tctttagtta
     3721 ttggtgtagg catcattgtt aaaatagtaa tgctgattat ttaaaaaaga aatgaactat
     3781 aactgaatat agagtcatgt cagtcaatag gaaagatatc ttggaatatg ctattcatat
     3841 gaagtataag aggagagtcg cagatgaaaa tagttattat aggtgggttt ttaggtggcg
     3901 gtaaaacgac tgtcttaaat catttgctcg ctgaatcatt aaaggagtcg ctgaaaccgg
     3961 cagttatcat gaatgaattt ggtaaaatga gtgttgatgg tgccttagta tctgaagaca
     4021 tacctttaag tgaactgaca gaggggtgta tctgttgtgc aatgaaagca gatgtatcag
     4081 aacaattaca tcaattatat ttaaaagagc aaccagacat tgtatttatt gaatgtagtg
     4141 ggattgcaga accggtctct gtcttagatg cttgtttaac gcctatttta gctccgttta
     4201 caaccattac acatatgatt ggtgttgtag atgcaagcat gtataaacat ataaaatcat
     4261 ttccaaaaga catccaaggc ttattttatg agcaattagc atattgttct gtcttatttg
     4321 ttaataaaat agattcagca gatgttgaaa caacgagcaa actattgaaa gatttagaag
     4381 ttattaaccc agaggcagat atacaagtcg gtatgcatgg cagcgtcact ttaccaatat
     4441 cagttagaca aatgacagtt acttccgaca ataaacatga atcattacac aaaacaataa
     4501 atcatcaatt tgtgcaatca ccagtcaaat gtactaaagc agagtttata aaacgtttag
     4561 catgccttcc gtctcatatt tataggttga aagggtttat gacatttgaa gacaccgcac
     4621 atacgtatct cattcaattt acacaaggac aatatgaatt aacacctgta gcattttcaa
     4681 aaaaagtgcc agaatatttg gtgcttatcg gaaagggtat ttcaaaagaa gactatcaat
     4741 gtttggaaca gtagtgtttt cagtgggaaa gaatgaacag tatatagata ataaatgccc
     4801 cacttgttat ataaattgta gtgagagggg catttataaa ttattttatt aggtatattt
     4861 ctttattgtt aaatacagat ctattaatag tatccataag gtcaaagata ataattgata
     4921 ttatttttaa agctacaaat aaatataaga taaattcagg tactatctat ttaa
//
LOCUS       DAHHOJ010000141         4868 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.142, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000141 DAHHOJ010000000
VERSION     DAHHOJ010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.142"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..378
                     /locus_tag="NBH32_002416"
     CDS             <1..378
                     /locus_tag="NBH32_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015973974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin MazF"
                     /protein_id="HCD1515985.1"
                     /translation="LFLNLISAQNNSAILKEVFDKKYQKNNTEFITKDYFSEFISDSL
                     EIENKLNKIDRNINNIVSAIDKVKKLKGNSYACINSFQPISKFRIRKVLPQKIKNPVI
                     DSSDIMLLINRINNNILQIPDIR"
     gene            569..1606
                     /locus_tag="NBH32_002417"
     CDS             569..1606
                     /locus_tag="NBH32_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCD1515986.1"
                     /translation="MKTRCYDGKKWQYEFKYEGKRYRKKGFRTKREANSAGLDKLNEL
                     RSGFNIDNYITLEEYFENWIKTYKQPVVKENTYRHYRNALQHIQKHKIGKMELSKINR
                     QVYQKFINDYSKEHAKETIRKTNGAIRSALDDALYDGLIFKNPAYKVNYKAGKPTKSE
                     QEKFISVTEYEILKDHVRKKRTRSSLALFIMICTGCRVSGARNIKIEHINQVKNTIFI
                     DERKTDTSPRYISIAKSDMKHIMDVISTFAISYDGYIFKEAGSIINLHAINNALKSAC
                     RVNNIPIITSHALRHTHCSYLLAKGVSIHYISKRLGHKNIAITTSVYSHLLEEKFNEE
                     DKKTTKILESM"
     gene            1657..2487
                     /gene="sph"
                     /locus_tag="NBH32_002418"
                     /pseudo
     CDS             1657..2487
                     /gene="sph"
                     /locus_tag="NBH32_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
     gene            complement(2725..3741)
                     /gene="lukG"
                     /locus_tag="NBH32_002419"
     CDS             complement(2725..3741)
                     /gene="lukG"
                     /locus_tag="NBH32_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit G"
                     /protein_id="HCD1515987.1"
                     /translation="MIKQLYKNITICTLALSTTFTVLPATSYAKINSEIKAVSEKNLD
                     GDTKMYTRTATTSDSQKNITQSLQFNFLTEPNYDKETVFIKAKGTIGSGLRILDPNGY
                     WNSTLRWPGSYSVSIQNVDDNNNTNVTDFAPKNQDESREVKYTYGYKTGGDFSINRGG
                     LTGNITKESNYSETISYQQPSYRTLLDQSTSHKGVGWKVEAYLINNMGHDHTRQLTND
                     SDNRTKSEIFSLTRNGNLWAKDNFTPKNKMPVTVSEGFNPEFLAVMSHDKKDEGKSKF
                     VVHYKRSMDEFKIDWNRHGFWGYWSGENHVDKKEEKLSALYEVDWKTHNVKFVKVLND
                     NEKK"
     gene            complement(3763..4815)
                     /gene="lukH"
                     /locus_tag="NBH32_002420"
     CDS             complement(3763..4815)
                     /gene="lukH"
                     /locus_tag="NBH32_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-component leukocidin LukGH subunit H"
                     /protein_id="HCD1515988.1"
                     /translation="MKNKKRVLIASSLSCAILLLSAATTQANSAHKDSQDQNKKEHVD
                     KSQQKEKRNVTNKDKNSTAPDDIGKNGKITKRTETVYDEKTNILQNLQFDFIDDPTYD
                     KNVLLVKKQGSIHSNLKFESHKEEKNSNWLKYPSEYHVDFQVKRNPKTEILDQLPKNK
                     ISTAKVDSTFSYSSGGKFDSTKGIGRTSSNSYSKTISYNQQNYDTIASGKNNNWHVHW
                     SVIANDLKYGGEVKNRNDELLFYRNTRIATVENPELSFASKYRYPALVRSGFNPEFLT
                     YLSNEKSNEKTQFEVTYTRNQDILKNRPGIHYAPPILEKNKEGQRLIVTYEVDWKNKT
                     VKVVDKYSDNKSFREG"
BASE COUNT         1681 a          768 c          707 g         1712 t
ORIGIN      
        1 ttgtttttaa atttaattag cgcgcaaaat aattcagcta tattaaaaga agttttcgat
       61 aaaaaatacc aaaaaaacaa cacagaattc atcactaaag attattttag tgaatttata
      121 tctgatagtt tagaaattga aaataaatta aataaaattg acagaaacat taataacata
      181 gtatcagcaa ttgataaggt aaaaaaatta aaaggtaata gttacgcttg cataaattct
      241 ttccagccga ttagtaagtt tcgcataaga aaagttttac cccaaaaaat taaaaatcca
      301 gtaatagatt cttcggatat tatgttactg ataaatagaa ttaataataa tatattgcag
      361 attcctgata taagatgata taattttaat atattaaagg tttatccttt aaaacacgta
      421 tatattcgtt accatttttg gtaattaacc atgtaatctt ataactataa gtggcgtctg
      481 tattttatac aggcgtcttt ttttatacaa ttttcatggg tagcccgcct acccttatta
      541 ttttttgcca attttgagga gggaacgcat gaaaacacgt tgttacgatg gtaaaaaatg
      601 gcaatatgaa tttaagtatg aaggaaaaag ataccgtaag aaaggtttta gaacaaagcg
      661 tgaagctaat tctgctggac tagacaagtt aaatgagtta agaagtggtt ttaatataga
      721 taactatata actcttgaag aatacttcga aaattggatt aaaacgtata aacaacctgt
      781 tgttaaagaa aatacctacc gtcattatag aaatgcatta caacatatac aaaaacataa
      841 aataggtaaa atggagttat caaagataaa tagacaagtt tatcagaaat tcataaacga
      901 ttattcaaaa gaacacgcaa aagaaactat aagaaaaaca aacggtgcta ttcggtcagc
      961 tttagatgac gcattatatg atgggcttat ttttaaaaat cccgcttata aagttaatta
     1021 taaagccgga aaacctacga agtcagaaca agaaaaattc atctcggtaa ctgaatatga
     1081 aatactaaaa gatcacgtca gaaagaagag aactcgttca tcattagcgc tattcataat
     1141 gatttgtacg ggttgtcgtg tcagtggtgc aagaaatata aagattgagc atatcaacca
     1201 agtgaaaaac actatattta ttgacgagcg aaaaaccgat acttccccta gatatatcag
     1261 tatcgctaaa tctgatatga aacacattat ggacgtcata agtacatttg caattagcta
     1321 tgatggttac attttcaaag aagccggatc tataattaac cttcatgcta tcaataatgc
     1381 tttgaaatca gcctgtagag ttaataatat accaattatt acatcgcacg cattaagaca
     1441 cactcattgt tcttatttac tagcaaaagg tgtatctata cattacattt ctaaaagatt
     1501 aggtcataaa aatatagcaa taactacatc cgtgtattct catttgttag aagaaaaatt
     1561 taatgaagag gacaaaaaaa caactaaaat tttagaaagt atgtaattta gggacccatt
     1621 agggactcca aacccaataa atactgttgt tacaaggttt ctatgtatcc aaactggggg
     1681 caatataaac gcgctgattt aatcggacaa tcttcttata ttaaaaataa tgatgtcgta
     1741 atattcaatg aagcatttga taatggcgca tcagataaat tattaagtaa tgtaaaaaaa
     1801 gaatatcctt atcaaacacc tgtactcggt cgttctcaat cagggtggga caaaactgaa
     1861 ggtagctact catcaactgt tgcagaagat ggtggcgtag cgattgtaag taaatatcct
     1921 attaaagaaa aaatccagca tgttttcaaa agcggttgtg gattcgataa tgatagcaac
     1981 aaaggctttg tttatacaaa aatagagaaa aacggtaaga acgttcacgt tatcggtaca
     2041 catacacaat ctgaagattc acgttgtggt gctggacatg atagaaaaat tagagctgaa
     2101 caaatgaaag aaatcagtga ctttgttaaa aagaaaaata tccctaaaga tgaaacggta
     2161 tatatcggtg gcgaccttaa tgttaataaa ggcactccag agttcaaaga tatgcttaaa
     2221 aacttgaatg taaatgatgt tctatatgca ggtcataata gcacatggga ccctcaatca
     2281 aattcaattg cgaaatataa ttaccctaat ggtaaaccag aacatttaga ctatatattt
     2341 acagataaag atcataaaca accaaaacaa ttagtcaatg aagttgtgac tgaaaaacct
     2401 aagccatggg atgtatatgc gttcccatat tactacgttt acaatgattt ttcagatcat
     2461 tatccaatca aagcctatag taaatagtgc tcaactaact aataactcgc ttcgttctaa
     2521 aaggtcgaag cgagttatat tgttaaaatt tgaattgact tacattttaa taaaatcatc
     2581 ttaacaattt taatttttca ttaatacaag tctttactct acactcaaac aagattcata
     2641 cactgcacat cataataaat ctatctactc aaatataaat aaaagttacc tactacattc
     2701 tatgtagcag gcaactttta ttacttattt cttttcatta tcattaagta cttttacaaa
     2761 cttcacatta tgtgtcttcc aatcaacttc atataatgct gataattttt cttctttttt
     2821 atctacatgg ttttcaccag accaatagcc ccagaaacca tgacgattcc aatctatttt
     2881 aaactcatcc attgatcttt tataatgaac aacaaatttt gatttacctt catctttttt
     2941 atcatgtgac ataacagcta aaaattctgg attaaaccct tcagaaacag ttacaggcat
     3001 cttattctta ggcgtaaaat tatctttcgc ccataaattt ccatttcgtg ttaaagaaaa
     3061 gatttcactt ttagttctat tatcactatc attagttaat tgtctcgtat ggtcatgtcc
     3121 catattattt atcaaatatg cttctacttt ccaacctaca cctttatgtg acgtagattg
     3181 atcaagtaat gtacgatacg atggttgttg ataactaatc gtctctgaat aattactctc
     3241 ttttgtaata tttccagtta accctccacg attaatcgaa aaatctccac ctgttttata
     3301 accatacgta tatttaactt ctcttgattc atcctgattt tttggtgcaa aatcagtcac
     3361 atttgtatta ttgttgtcat caacattttg aattgaaact gaataagatc caggccatct
     3421 taatgtacta ttccaataac catttgggtc tagaattctt aaaccactac ctattgttcc
     3481 tttcgcttta ataaatactg tttctttatc ataattaggt tcagttaaga agttaaattg
     3541 taagctttga gtaatatttt tttgactatc acttgttgta gctgtacgtg tatacatttt
     3601 agtatcacca tcaagattct tctcagaaac agctttaatt tcagaattaa tttttgcata
     3661 agaagtagct ggtaatacag tgaaagtagt cgatagtgct aacgtacaaa ttgtgatatt
     3721 tttatatagt tgtttaatca tttgtaatcc ttcccttcga ttttatcctt ctctaaatga
     3781 tttattatca gagtatttat caacgacttt aactgtttta tttttccaat caacttcata
     3841 agtgacaatt aatctttgac cttctttatt tttttctaaa attggaggtg cataatgtat
     3901 tccaggtctg tttttcaaaa tatcttgatt tcgtgtgtat gttacttcaa attgcgtttt
     3961 ctcatttgac ttttcattag ataaataagt taaaaattct ggattaaagc cacttcttac
     4021 taatgctggg tatctatatt ttgaagcaaa gcttagttca gggttttcaa cagtagcaat
     4081 tctcgtattt ctatagaata ataattcatc atttctattt ttcacttctc caccatactt
     4141 caagtcattc gcaataactg accagtgtac atgccagtta ttatttttac cgctggcaat
     4201 tgtgtcataa ttttgctgat tataactaat cgttttggag tagctatttg atgaagttcg
     4261 tccaatacct ttcgttgaat cgaatttacc acctgagcta tatgaaaatg tactgtctac
     4321 tttcgcagtt gaaattttat ttttcggcaa ttggtctaat atttcagttt taggatttct
     4381 ttttacttga aaatctacat ggtactcact tggatacttt aaccaatttg aatttttttc
     4441 ttctttatga gattcaaact ttaaatttga atgaattgag ccttgttttt taacaagtaa
     4501 tacattcttg tcataagttg gatcatcgat aaagtcgaat tgtaaatttt ggagtatatt
     4561 tgttttctca tcatatactg tttcagttcg ttttgtgatt ttaccgtttt tcccaatatc
     4621 atccggtgct gttgaatttt tatctttatt agtaacatta cgtttctctt tttgttgaga
     4681 cttatcaaca tgttctttct tattttggtc ttgcgagtct ttatgagctg aatttgcttg
     4741 agtcgttgct gctgataaca ataaaattgc acatgataat gatgacgcta ttaaaacacg
     4801 ttttttattt ttcattgtta taaccttctt tcgtatgatt gatatttgtt gatatgtatc
     4861 gacatgtg
//
LOCUS       DAHHOJ010000142         4847 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.143, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000142 DAHHOJ010000000
VERSION     DAHHOJ010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4847
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.143"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            97..969
                     /gene="dprA"
                     /locus_tag="NBH32_002421"
     CDS             97..969
                     /gene="dprA"
                     /locus_tag="NBH32_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000593194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCD1515989.1"
                     /translation="MIRLFLLKLYWSHFSTKQIHQFLMAYPNVIKEEGRKKDSYLCEW
                     VNREENVHLLRKYYAFIKLDHNDIIKELQKLKVSYITYMDSEYPVLLKEIYQFPLLLF
                     YKGNIKLINNMHHLAVVGARDSTSYTQQSLEFLLSNDKSKYLTIVSGLAQGADAMAHQ
                     IALKYNLPTIAVLAFGHQTHYPKSTLALRNKIEEKGLVISEYPPHTPIAKYRFPERNR
                     IISGLSKGVLITEAKEQSGSHITIDFALEQNRNVYVLPGSMFNPMTKGNLLRIQEGAK
                     VVLNANDIFEDYYI"
     gene            1143..3218
                     /gene="topA"
                     /locus_tag="NBH32_002422"
     CDS             1143..3218
                     /gene="topA"
                     /locus_tag="NBH32_002422"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCD1515990.1"
                     /translation="MTLADNLVIVESPAKAKTIEKYLGKKYKVIASMGHVRDLPRSQM
                     GVDTEDNYEPKYITIRGKGPVVKELKKHAKKAKNVFLASDPDREGEAIAWHLSKILEL
                     EDSKENRVVFNEITKDAVKESFKNPREIEMNLVDAQQARRILDRLVGYNISPVLWKKV
                     KKGLSAGRVQSVALRLVIDRENEIRNFKPEEYWTIEGEFRYKKSKFNAKFLHYKNKPF
                     KLKTKKDVEKITAALDGDQFEITNVTKKEKTRNPANPFTTSTLQQEAARKLNFKARKT
                     MMVAQQLYEGIDLKKQGTIGLITYMRTDSTRISDTAKAEAKQYITDKYGESYTSKRKA
                     SGKQGDQDAHEAIRPSSTMRTPDDMKSFLTKDQYRLYKLIWERFVASQMAPAILDTVS
                     LDITQGDIKFRANGQTIKFKGFMTLYVETKDDSDSEKENKLPKLEQGDKVTATQIEPA
                     QHYTQPPPRYTEARLVKTLEELKIGRPSTYAPTIDTIQKRNYVKLESKRFVPTELGEI
                     VHEQVKEYFPEIIDVEFTVNMETLLDKIAEGDITWRKVIDGFFSSFKQDVERAEEEME
                     KIEIKDEPAGEDCEVCGSPMVIKMGRYGKFMACSNFPDCRNTKAIVKSIGVKCPKCND
                     GDVVERKSKKNRVFYGCSKYPECDFISWDKPIGRDCPKCNQYLVENKKGKTTQVICSN
                     CDYKEAAQK"
     gene            3374..4681
                     /gene="trmFO"
                     /locus_tag="NBH32_002423"
     CDS             3374..4681
                     /gene="trmFO"
                     /locus_tag="NBH32_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HCD1515991.1"
                     /translation="MTQTVNVIGAGLAGSEAAYQLAERGIKVNLIEMRPVKQTPAHHT
                     DKFAELVCSNSLRGNALTNGVGVLKEEMRRLNSIIIEAADKARVPAGGALAVDRHDFS
                     GYITETLKNHENITVINEEINAIPDGYTIIATGPLTTETLAQEIVDITGKDQLYFYDA
                     AAPIIEKESIDMDKVYLKSRYDKGEAAYLNCPMTEDEFNRFYDAVLEAEVAPVNSFEK
                     EKYFEGCMPFEVMAERGRKTLLFGPMKPVGLEDPKTGKRPYAVVQLRQDDAAGTLYNI
                     VGFQTHLKWGAQKEVIKLIPGLENVDIVRYGVMHRNTFINSPDVLNEKYELISQPNIQ
                     FAGQMTGVEGYVESAASGLVAGINLAHKILGKGEVVFPRETMIGSMAYYISHAKNNKN
                     FQPMNANFGLLPSLETRIKDKKERYEAQANRALDYLENFKKTL"
BASE COUNT         1858 a          705 c          896 g         1388 t
ORIGIN      
        1 ccttaaaata acattacatc gacaatgttt ttgtattaga aatgtaaaac aaaaatagaa
       61 tatgattttg tatgattaca aaaaggagtt gatttattga ttagactatt tttgcttaag
      121 ttatactggt cacacttttc gactaaacaa attcatcaat ttttaatggc atatcctaat
      181 gtaattaaag aggagggaag aaaaaaagat agttatttat gtgaatgggt gaatagggaa
      241 gaaaatgttc atttattacg taaatactat gcttttataa aacttgatca taacgatatt
      301 attaaagaac tgcagaaatt aaaagtaagt tacattacat atatggattc tgaataccca
      361 gtgctattaa aagaaatata tcaatttcca ttacttcttt tctataaagg gaacatcaaa
      421 ttaataaata atatgcatca tttggcagta gtaggtgcaa gagattctac aagttatacc
      481 caacagtctt tagaattttt attatcaaat gataaaagca aatatttaac aattgtttcc
      541 ggccttgctc aaggagctga tgcaatggca catcaaatag ctttaaaata caatctccct
      601 acaattgcag ttttagcctt tggccatcaa acacattatc ccaaaagtac attagcatta
      661 agaaataaaa tagaagaaaa aggtttagtt atatccgaat atccaccaca tacaccaatt
      721 gctaaatata gatttcctga gcgcaataga attatcagcg gtttgtcaaa aggggtttta
      781 attactgagg ctaaggaaca aagtggcagt cacatcacga tagattttgc attagagcaa
      841 aatagaaatg tttatgtttt acctggatct atgtttaatc ctatgacaaa aggtaattta
      901 ttacgtatcc aagaaggtgc taaggtagta ttaaacgcta atgatatatt tgaagactac
      961 tatatttaaa actaaaaata acgagttgct tcatatttct tttgttaccc tttgtaattt
     1021 attttttaat aaaatctata aaaaatagac agggaaaatg atttgtttag atataaaacg
     1081 ttgacaaaag caaaattaag cgtttatcat ttatctttag taattagatt agcgaggggg
     1141 aaatgacatt ggcagataat ttagtcattg ttgaatcgcc tgcaaaagca aaaaccattg
     1201 aaaagtattt aggtaagaaa tataaagtta tagcttcaat gggacacgtc agagacttac
     1261 caagaagtca aatgggtgtc gacactgaag ataattacga accaaaatat ataacaatac
     1321 gcggaaaagg tcctgttgta aaagaattga aaaagcatgc aaaaaaagcg aaaaacgtct
     1381 ttctcgcaag tgaccccgac cgtgaaggtg aagcaattgc ttggcattta tcaaaaattt
     1441 tagagcttga agattctaaa gaaaatcgcg ttgttttcaa cgaaataact aaagacgctg
     1501 ttaaagaaag ttttaaaaat cctagagaaa ttgaaatgaa cttagtcgat gcacaacaag
     1561 cgcgtcgaat attagataga ttggttggct ataacatctc gccagttcta tggaaaaaag
     1621 taaaaaaagg gttgtcagcg ggtcgagttc aatctgttgc acttcgttta gtcattgacc
     1681 gtgaaaatga aattcgaaac tttaaaccag aagaatattg gactattgaa ggagaattta
     1741 gatacaaaaa atcaaaattc aatgctaaat tccttcatta taaaaataaa ccttttaaat
     1801 taaaaacgaa aaaagatgtt gagaaaatta cagctgcatt agatggagat caattcgaaa
     1861 ttacaaacgt gactaaaaaa gaaaaaacgc gtaatccagc aaacccattt acaacttcta
     1921 cattacaaca agaggcggca cgtaaattaa actttaaagc tagaaaaaca atgatggtcg
     1981 cacaacaatt atatgaaggt atagatttga aaaaacaagg tacgattggt ttaataacat
     2041 atatgagaac cgattctaca cgtatttcag atactgccaa agctgaagca aaacagtata
     2101 taactgataa atacggtgaa tcttacactt ctaaacgtaa agcatcaggg aaacaaggtg
     2161 accaagatgc ccatgaggct attagacctt caagtactat gcgtacgcca gatgatatga
     2221 agtcattttt aacgaaagac caataccgat tatacaaatt aatttgggaa cgatttgttg
     2281 ctagtcaaat ggctccagca atacttgata cagtctcatt agacataaca caaggtgaca
     2341 ttaaatttag agcgaatggt caaacaatca agtttaaagg atttatgaca ctctatgtag
     2401 aaactaaaga tgatagtgat agcgaaaagg aaaataaact gcctaaatta gagcaaggtg
     2461 ataaagtcac agcaactcaa attgaaccag ctcaacacta tacacaacca cctccaagat
     2521 atactgaggc gagattagta aaaacacttg aagaattgaa aattgggcga ccatcaacat
     2581 atgcaccgac aatagatacg attcaaaagc gtaactatgt caaattagaa agtaagcgtt
     2641 ttgttcctac tgagttggga gaaatagttc atgaacaagt gaaagaatac ttcccagaga
     2701 ttattgatgt ggaattcaca gtgaatatgg aaacgttact tgataagatt gcagaaggcg
     2761 acattacatg gagaaaagta atcgacggtt tctttagtag ctttaaacaa gatgttgaac
     2821 gtgctgaaga agagatggaa aagattgaaa tcaaagatga gccagccggt gaagactgtg
     2881 aagtttgtgg ttctcctatg gttataaaaa tgggacgcta tggtaagttc atggcttgct
     2941 caaacttccc ggattgtcgt aatacaaaag cgatagttaa gtctattggt gttaaatgtc
     3001 caaaatgtaa tgatggtgac gtcgtagaaa gaaaatctaa aaagaatcgt gtcttttatg
     3061 gatgttcgaa atatcctgaa tgcgacttta tctcttggga taagccgatt ggaagagatt
     3121 gtccaaaatg taaccaatat cttgttgaaa ataaaaaagg caagacaaca caagtaatat
     3181 gttcaaattg cgattataaa gaggcagcgc agaaataata tttttatttc ctagagacaa
     3241 tttaagaatg ttaaatagaa tcattagtga atcttatttt aaagatagta atggattaat
     3301 ctaaataagt gcggataata taaacataac aacataatta aaagacataa atgacaataa
     3361 aaggagtata gaaatgactc aaactgtaaa tgtaataggt gctggtcttg ccggttcaga
     3421 agcggcatat caattagctg aaagaggaat taaagttaat ctaatagaga tgagacctgt
     3481 taaacaaaca ccagcgcacc atactgataa atttgcggaa cttgtatgtt ccaattcatt
     3541 acgcggaaat gctttaacta atggtgtggg tgttttaaaa gaagaaatga gaagattgaa
     3601 ttctataatt attgaagcgg ctgataaggc acgagttcca gctggtggtg cattagcagt
     3661 tgatagacac gatttttcag gttatattac tgaaacactc aaaaatcatg aaaatatcac
     3721 agttattaat gaagaaatta atgccattcc agatggatac acaattatcg caacaggacc
     3781 acttactaca gaaacgcttg ctcaagaaat agtggacatt actggtaaag atcaacttta
     3841 tttctatgat gcagctgctc caattattga aaaagaatct attgatatgg ataaagttta
     3901 cttaaagtcc cgttatgata aaggtgaagc tgcatattta aactgtccta tgactgagga
     3961 tgaatttaat cgtttttatg atgcagtatt agaagctgaa gttgcgcctg taaattcatt
     4021 tgaaaaagaa aaatatttcg agggttgtat gccttttgaa gtaatggcag aacgcggacg
     4081 caagacatta ctatttggac caatgaaacc agtaggatta gaagatccaa agactgggaa
     4141 acgtccttat gcagtggttc aattaagaca agatgacgct gctggcacac tctacaatat
     4201 tgtcggcttc caaacgcatt taaaatgggg agctcaaaaa gaagttatta aattaattcc
     4261 aggcttagaa aatgttgata ttgttagata tggtgtgatg catagaaata ccttcattaa
     4321 ctcaccggac gtattaaacg agaaatatga attgatttca caaccaaaca tacagtttgc
     4381 gggacaaatg actggtgttg aaggttatgt ggaaagcgca gctagtggct tagttgcagg
     4441 catcaatctt gcgcataaaa tattaggcaa aggtgaggta gtatttccga gagaaactat
     4501 gattggaagt atggcttact atatttctca tgctaaaaac aataagaatt tccaacctat
     4561 gaatgctaac tttgggttat taccatcttt agaaactaga attaaagata aaaaagaacg
     4621 ctatgaagca caagctaata gagctttgga ttacttagaa aatttcaaaa aaactttata
     4681 aaatagttag aaagactaga tatgctattc attcttaagt catcaacgag taagtaatga
     4741 ctttctaaat ggaaaatact tatcctagtc tttttaattt tggaattgtt acgtatttct
     4801 gacaattgag aattcgcatt caaaaaatat ctaaataaat aacacgc
//
LOCUS       DAHHOJ010000143         4831 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.144, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000143 DAHHOJ010000000
VERSION     DAHHOJ010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4831)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4831)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4831
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.144"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..1005)
                     /locus_tag="NBH32_002424"
     CDS             complement(<1..1005)
                     /locus_tag="NBH32_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCD1515992.1"
                     /translation="MNKTKGFTKYKKMRYMPGLDGLRAIAVLGIIIYHLNKQWLTGGF
                     LGVDTFFVISGYLITSLLLKEYDDTGIIKLKSFWIRRLKRLLPAVIVLLMVVGTATLL
                     LKSDNIIRVKHDIIAAIFYVSNWWYIAKDVNYFEQFSFMPLKHLWSLAIEEQFYIFFP
                     VILVTLLLTIKKRYKIGFIFWGVSIISLGLMMFIYSINGDHSRVYFGTDTRLQTLLLG
                     VILAFLWPPFKLKNDPPKVVKYVIDSIGSLSFIILILLFFIINDETNWIYDGGFYLIS
                     ILTLFIIASVVHPSTWIAKIFSNPVLVFIGKRSYSLYLWHFAVISFVHSYYVDGQIPV
                     Y"
     gene            complement(1660..1968)
                     /locus_tag="NBH32_002425"
     CDS             complement(1660..1968)
                     /locus_tag="NBH32_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002465312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HCD1515993.1"
                     /translation="MEEALKDSILGALEMVIDPELGIDIVNLGLVYKVNVDDEGVCTV
                     DMTLTSMGCPMGPQIIDQVKTVLAEIPEIQDTEVNIVWSPPWTKDMMSRYAKIALGVS
                     "
     gene            2082..2906
                     /locus_tag="NBH32_002426"
     CDS             2082..2906
                     /locus_tag="NBH32_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1515994.1"
                     /translation="MQPHLICLDLDGTLLNDNKEISSYTKQVLNELQQRGHQIMIATG
                     RPYRASQMYYHELNLTTPIVNFNGAYVHHPKDKNFKTCHEILDLGIAQNIIQGLQQYQ
                     VSNIIAEVKDYVFINNHDPRLFEGFSMGNPRIQTGNLLVHLKESPTSILIEAEESKIP
                     EIKNMLTHFYADHIEHRRWGAPFPVIEIVKLGINKARGIEQVRQFLNIDRNNIIAFGD
                     EDNDIEMIEYARHGVAMENGLQELKDVANNITFNNNEDGIGRYLNDFFNLNIRYYC"
     gene            2958..4274
                     /locus_tag="NBH32_002427"
     CDS             2958..4274
                     /locus_tag="NBH32_002427"
                     /EC_number="1.8.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-disulfide reductase"
                     /protein_id="HCD1515995.1"
                     /translation="MPKIVVVGAVAGGATCASQIRRLDKESDIIIFEKDRDMSFANCA
                     LPYVIGEVVEDRKYALAYTPEKFYDRKQITVKTYHEVIAINDERQTVTVLNRKTNEQF
                     EESYDKLILSPGASANSLGFESDITFTLRNLEDTDAIDQFIKANQVDKVLVVGAGYVS
                     LEVLENLYERGLHPTLIHRSDKINKLMDTDMNQPILDELDKREIPYRLNEEIDAINGN
                     EITFKSGKVEHYDMIIEGVGTHPNSKFIESSNIKLDRKGFIPVNDKFETNVPNIYAIG
                     DIATSHYRHVDLTASVPLAWGAHRAASIVAEQIAGNDTIEFKGFLGNNIVKFFDYTFA
                     SVGVKPNELKQFDYKMVEVTQGAHANYYPGNSPLHLRVYYDTSNRQILRAAAVGKEGA
                     DKRIDVLSMAMMNQLTVDELTEFEVAYAPPYSHPKDLINMIGYKAK"
     gene            complement(4445..>4831)
                     /locus_tag="NBH32_002428"
     CDS             complement(4445..>4831)
                     /locus_tag="NBH32_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YisL family protein"
                     /protein_id="HCD1515996.1"
                     /translation="LHLHILSWVLAIILFIATYLNISKNQGGSPFFKPLHMILRLFML
                     LTLISGFWILIQSFMNGGANHMLLTLKMLCGVAVVGLMEVSIAKRKRHEQSHTMFWIT
                     IALIIITMVLGVILPLGPISKLFGIG"
BASE COUNT         1764 a          821 c          733 g         1513 t
ORIGIN      
        1 atatacaggt atctgtccgt ctacatagta actatgtacg aaactaatta ctgcaaaatg
       61 ccataaatat aaactataag accttttccc gataaatact aacactggat ttgaaaatat
      121 cttcgctatc catgtagatg gatgaacgac actggcaata ataaataacg ttaatatgga
      181 tattaaatag aaaccaccat catatatcca attcgtctca tcattaataa tgaaaaataa
      241 taatataagt attataaatg ataaactacc tatgctatca ataacatatt ttacaacttt
      301 aggtggatca tttttcaatt taaacggtgg ccataaaaaa gctaaaataa cacccagtaa
      361 caatgtctgt aatctagtat ctgtaccaaa atacactcgt gaatgatccc cattaatact
      421 gtagataaac atcattaacc ctaaagaaat tattgatact ccccaaaaaa taaatcctat
      481 tttgtatcgc tttttaattg ttaacaataa tgtaaccaaa ataactggga aaaatatgta
      541 aaactgttct tcaattgcta aagaccataa atgctttaat ggcataaatg aaaattgctc
      601 aaaataatta acatcttttg ctatatacca ccagtttgat acataaaata tcgcagcaat
      661 aatatcatgt ttaaccctaa tgatattatc tgattttaat aataaggttg ctgtccctac
      721 aaccattaat aaaactatga ctgctggtaa taaacgtttt aaacgacgta tccaaaagct
      781 tttcaattta atgatacctg tgtcatcata ctcttttagt aataagctcg taattaaata
      841 accagagatc acaaaaaatg tatccacacc taaaaagcca cctgtcaacc attgcttatt
      901 taagtggtaa ataataattc ctagaacagc gattgccctc aaaccatcga gccctggcat
      961 atatctcatt ttcttatact ttgtaaaacc ctttgttttg ttcatttttg cattcttccc
     1021 ttttaaaact gttcttctta gatgcttaat taaatttagt tatgctgttt aaaagaatat
     1081 tgaaatgcat atgtatatta ttgaattacg acatcatcaa aatcatattg actaaaatac
     1141 tgttaaatta aaaaaattac caatgatgat tcttacttcg aaatccaatt tgtaatgcaa
     1201 ctcggcaact taaaaactat gaagtattat gtattgtaat ataactgtaa tataaattca
     1261 atttattata aaaattttca agaaaatatt caactagaaa atgaattgtg cgctcttaga
     1321 agtgagaaac actgtcttaa ttcatatttt ttgaaacaag ttagatataa attttcaaaa
     1381 taaaatcaga acctagaaca taaataaggc tcccttcaaa attttcattt ttcaatgtct
     1441 actttgaagg gagcttattc acaatgaatt atactctaca aggttatatt gactgcgggg
     1501 cccaaacaca gagaatttca aaaagaaatt ctacaagcaa tgcgagttgg ggagcgggcc
     1561 caaacacaga gaatttcgaa aagaaattct acaagcaatg caagttggga gcggatcccc
     1621 aattggattt ccaatttcta caggcaatgc acgtttatgt tagctcacac caagtgcaat
     1681 cttagcgtaa cgtgacatca tatcttttgt ccaaggtgga ctccatacga tattcacttc
     1741 agtatcctga atttcaggaa tctctgctaa tactgtttta acttgatcaa taatttgagg
     1801 tcccattgga catcccattg atgttaaagt catatcaact gtacatacgc cttcatcatc
     1861 aacattcact ttgtatacta aacctaaatt aacgatatca attcctaatt cagggtcaat
     1921 taccatttct aatgcaccta agatactatc tttcaatgcc tcttccatcc atatcacctc
     1981 tttaatgtca tattattcat aatatatcaa atatccgaca aaacgccaat aaaatgctat
     2041 gatgtatcta tatgaactaa gcaacttatg aggagagaga tatgcaacca catttaatat
     2101 gtctagactt agacggaaca ttattaaacg ataacaaaga aatttcatca tatactaaac
     2161 aagtattaaa tgaattacaa caacgtggac accaaattat gattgcgact ggcagacctt
     2221 atcgtgcaag tcaaatgtat tatcatgaat taaatttaac gacaccaatt gttaatttta
     2281 atggcgctta cgtacatcac cctaaagata aaaacttcaa aacttgccat gaaattttag
     2341 atttaggcat cgcacaaaac attattcaag gattacaaca atatcaagta tcgaatatta
     2401 tagcagaagt gaaagattat gttttcatta acaatcatga tccaagatta tttgaaggtt
     2461 tttcaatggg taatccaaga attcaaactg gtaatttact tgtccacttg aaagaatccc
     2521 ctacctcaat tttaattgaa gccgaagaaa gtaaaatacc tgaaatcaaa aatatgctta
     2581 ctcattttta tgccgatcat attgagcatc gacgctgggg cgcaccattc cctgtcattg
     2641 aaattgtaaa acttggtatt aataaagcaa gaggcattga gcaagttaga caatttttaa
     2701 atattgaccg aaataatatt attgcattcg gtgatgaaga taatgatatt gaaatgattg
     2761 agtacgcccg ccatggtgtt gctatggaaa atggtttgca agaacttaaa gatgtagcga
     2821 ataatattac attcaacaat aatgaagatg gcattggtcg atatttgaat gatttcttta
     2881 atttaaatat tagatattac tgttaattta taactaatca ttttataata ttttaaaaca
     2941 ataggaggta agttacgatg cccaaaatag tcgtagtcgg agcggtcgct ggcggtgcaa
     3001 catgtgccag ccaaattcga cgtttagata aagaaagtga cattattatt tttgaaaaag
     3061 atcgtgatat gagctttgct aattgtgcat tgccttatgt cattggcgaa gttgttgaag
     3121 atagaaaata tgctttagcg tatacacctg aaaaatttta tgatagaaag caaatcacag
     3181 taaaaactta tcatgaagtt attgcaatca atgatgaaag acaaactgta actgtattaa
     3241 atagaaagac caacgaacaa tttgaagaat cttacgataa actcatttta agccctggtg
     3301 caagtgcaaa tagccttggc tttgagagtg atattacatt tacacttaga aatttagaag
     3361 acactgatgc tatcgatcaa ttcatcaaag caaatcaagt tgataaagta ttggttgtag
     3421 gtgcagggta tgtttcatta gaagttcttg aaaatcttta tgaacgtggt ttacacccta
     3481 ctttaattca tcgatctgat aagataaata aattaatgga taccgacatg aatcaaccta
     3541 tacttgatga attagataag cgggagattc cataccgttt aaatgaggaa attgatgcta
     3601 tcaatggaaa tgaaattaca tttaaatcag gaaaagttga acattacgat atgattattg
     3661 aaggtgtcgg tactcacccc aattcaaaat ttatcgaaag ttcaaatatc aaacttgatc
     3721 gaaaaggctt cataccggta aacgataaat ttgaaacaaa tgttccaaac atttatgcaa
     3781 taggagatat tgcaacatca cattatcgac atgtcgatct aacggctagt gttcctttag
     3841 cttggggcgc tcaccgtgca gcaagtattg ttgccgaaca aattgctgga aatgacacta
     3901 ttgaattcaa aggcttctta ggcaacaata ttgtgaagtt ctttgattat acatttgcga
     3961 gtgtcggcgt taaaccaaac gaactgaagc aatttgacta taaaatggtg gaagtcactc
     4021 aaggtgcaca cgcgaattat tacccaggaa attccccttt acacttaaga gtatattatg
     4081 acacttcaaa ccgtcagatt ttaagagcag ctgcagtagg aaaagaaggt gcagataaac
     4141 gtattgatgt actatcgatg gcaatgatga accagctaac tgtagatgag ttaactgagt
     4201 ttgaagtggc ttatgcacca ccatatagcc accctaaaga tttaatcaat atgattggtt
     4261 acaaagctaa ataaatgaat catgcgattc aatctaatct taattgtcta ggttagaatt
     4321 atgttggact ggtactacta tccagtccat tttttatgtt taacattttt aaaatcaaaa
     4381 aagacataag gtcttggact aataattgtc catgccttat gtcataaact atatgtctta
     4441 tcaattagcc aataccgaat aattttgata taggccctaa cggtagaatg acacctaata
     4501 ccattgtgat gataattaat gcaattgtta tccaaaacat tgtgtgactt tgttcatgtc
     4561 tctttctttt agcaatcgac acttccatca atccaactac tgcaacacca cacagcattt
     4621 tcaatgtaag caacatatga tttgccccgc cattcataaa tgactgaatt aatatccaaa
     4681 atcctgaaat taacgtcaac agcataaata agcgtaaaat catgtgcaac ggtttgaaaa
     4741 atggtgatcc gccttgattt tttgaaatgt ttaagtatgt agcgataaat aaaataatcg
     4801 ctaataccca acttaatata tgtaaatgta a
//
LOCUS       DAHHOJ010000144         4796 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.145, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000144 DAHHOJ010000000
VERSION     DAHHOJ010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4796
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.145"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(164..1168)
                     /locus_tag="NBH32_002429"
     CDS             complement(164..1168)
                     /locus_tag="NBH32_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-dependent carboxyltransferase family
                     protein"
                     /protein_id="HCD1515997.1"
                     /translation="MSIIIEKSGLFSSFQDFGRRGYEHDGVIPCGALDTLAHEIANRL
                     VANDKNEATLEMTNKMAKIRFTEPTLIALAGGNVKAYTEHMTISPYKLYLLDKGDVLK
                     FRETSYTSRVYLAVGGGFELDAWLGSNSTDFNVKIGGFKGRTLQDGDEIKLKRDYTAR
                     HHKLFENLAHTKQTDWGIDGYALSFNYMSDVFHVIKNKGTEDFKEDAIQRFVKHDYKV
                     TSKANRMGMMLEGEKIKAFYEDMPPYQTVKKGTIQIKRDGTPIILLNDHYTLGSYPQI
                     GTIASYHLTKLAQKPQGSRLKFQFIDILTAEKNLVKYSNWLNQLFHGIEYRMQLEMMK
                     "
     gene            complement(1152..1862)
                     /locus_tag="NBH32_002430"
     CDS             complement(1152..1862)
                     /locus_tag="NBH32_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allophanate hydrolase subunit 1"
                     /protein_id="HCD1515998.1"
                     /translation="MKIYSQGDQAIVVAIEKEVSKSLTEDLLTLRSYLIEQNYPFIIE
                     IVPSESDMMIVYDARDMIKHHYIQSPFLYMKALIESIHLNIKHDFNQQDLIEIPIVYG
                     SKYGPDLESLLKHYKIKLETFIELHSKAQYFVSMMGYSPGFPYLTGLNKKLYINHTSK
                     QKKFIPAGSVVLEGKKCGIVTTDTINDWLVIGYTPLSLFNPKESDFARLKLGDNIKFR
                     PINENELEVGAFKDVNHN"
     gene            complement(1952..2158)
                     /locus_tag="NBH32_002431"
     CDS             complement(1952..2158)
                     /locus_tag="NBH32_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1515999.1"
                     /translation="MIIVYIVLLLILVYVNYRLVNRLLSENRIYVVRLIATITTVISF
                     ILVYALIHELMPFVVRAMDLMYHQ"
     gene            complement(2224..2832)
                     /locus_tag="NBH32_002432"
     CDS             complement(2224..2832)
                     /locus_tag="NBH32_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HCD1516000.1"
                     /translation="MQLFETMKIDNGHIPRLTYHTNRIKCSSERLNFKFDEHAWRNEL
                     NDVTTKYHSGQYRLKIVLNAESEFETIVSPLPEKSSFTAKFQVLPKAVNPTFINNKTT
                     ERKHLAHNHETDLILLTSEDGKVLEFDIGNIVIEEDGKWYTPSYKDDFLKGCMRDYLI
                     DSDKLVEKDFNKNELIYKYHNNEIRLFLINSLREVADVHLCL"
     gene            complement(2832..3983)
                     /gene="pabB"
                     /locus_tag="NBH32_002433"
     CDS             complement(2832..3983)
                     /gene="pabB"
                     /locus_tag="NBH32_002433"
                     /EC_number="2.6.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component I"
                     /protein_id="HCD1516001.1"
                     /translation="MRIEYNYRYYLTENEYKQYHIQLKGFIKKYVATKLADVGEVIHF
                     AQAQQRQGRYVSLYLSYEAAKYFNHVMCTHSLAKDDIYAVAYSFEKAESINSTYEHQT
                     SYVSKHHFSFVESSEVMMTNIKRVQQAIVEGETYQVNYTARLTDNIYYPISTLYERLT
                     QFSNGNYTALLQTDEIQVASISPELFFQKGQFNNVDNVIISKPMKGTMPRGKTEAEDQ
                     QYYKTLQTSSKDRAENVMIVDLLRNDIGRISQSGSIKVYKLFFIEAYKTVFQMTSMVS
                     GTLKNNTDLTQILTSLFPCGSITGAPKLNTMKYIKQLESSPRGIYCGAIGLLLPTEDD
                     KMIFNIPIRTIEYKYGQAIYGVGAGITIDSKPKDEVNEFYAKTKILEML"
     gene            complement(3967..4560)
                     /locus_tag="NBH32_002434"
     CDS             complement(3967..4560)
                     /locus_tag="NBH32_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HCD1516002.1"
                     /translation="MILVIDNNDSFTYNLIDYIKTQTKLTVQVVGIDNLLIEDVINMK
                     PKAIVISPGPGNPDDYPILNEVLEQFYQRVPILGVCLGFQCIVSYFGGNIIHGYHPVH
                     GHTTQLRHTNEGIFQGLPQNFNVMRYHSLIADGATFPNCLKITAKNDAAIIMAFEHIR
                     FPVFGVQYHPESILSEYGYRQVELFLSKVGDYCENRI"
BASE COUNT         1521 a          852 c          663 g         1760 t
ORIGIN      
        1 taattttagt caaaattcaa gtaaaaacac actttatctt ttcattttac tgtgaaacga
       61 atttgaatat gatacatttc gcagataaaa cacagtatta tttcattata cattaggagg
      121 aaattatttg catcggactc gagtatgagc tacgtaccaa atattatttc atcatttcta
      181 attgcattct atattctatt ccatggaata attgatttaa ccagttacta tacttaacaa
      241 ggttcttttc agccgttaaa atatctataa attgaaattt caaacgtgat ccttgcggtt
      301 tttgtgctaa tttcgttaaa tgataacttg cgattgtacc gatttgcgga tagctaccta
      361 gcgtataatg atcatttaat aagataatag gtgtgccatc acgcttaatt tgtatcgttc
      421 cttttttgac agtctgatac ggtggcatat cttcataaaa ggctttgatt ttttcacctt
      481 caagcatcat ccccatgcga tttgctttgc tcgttacttt ataatcatgt ttcacaaatc
      541 tttgaatggc atcttcttta aaatcttccg tacctttatt tttaatgaca tgaaatacat
      601 cagacatata attaaatgac aaggcgtatc catcaatacc ccaatctgtt tgtttcgtgt
      661 gagcaaggtt ttcaaataac ttatgatgac gagctgtata atctctctta agctttattt
      721 catcgccatc ttgtaatgtt cttcctttaa aaccaccaat ttttacatta aagtcggttg
      781 aattagatcc taaccatgca tctaattcaa atccgcctcc cacagctaaa tacactcgcg
      841 atgtataact tgtttctcta aactttaaaa catcgccttt atctaacaaa tacaatttat
      901 atggagatat agtcatatgc tcagtgtatg ctttgacatt accccctgct aatgcaatca
      961 gcgtaggttc tgtaaaacga atctttgcca ttttattagt catttccaaa gttgcttcat
     1021 tcttgtcatt agcaactaat cgattagcaa tttcatgtgc taaagtatca agtgcaccac
     1081 atggaattac accatcatgt tcatatcccc ttctaccaaa gtcctgaaag ctactgaata
     1141 agccactttt ttcaattatg attgacatct ttaaacgctc ctacttctaa ttcattttca
     1201 ttgataggtc taaatttaat attatcgcct aactttaagc gtgcgaaatc tgattctttc
     1261 ggattaaaaa gtgataatgg tgtataacca ataactaacc aatcattaat tgtatccgtt
     1321 gttacaatac cgcatttttt cccttcaagt actacagaac cagctggaat gaattttttc
     1381 tgtttactcg tgtgattaat atacaatttc ttatttaatc cagttaaata aggaaaccca
     1441 ggtgaatatc ccatcatcga aacaaaatat tgcgccttag aatgtaattc aataaaagtt
     1501 tctagcttga ttttgtaatg ttttaaaagt gattctaaat ccggaccata tttcgaacca
     1561 tacacaattg gtatttcaat caaatcttgc tggttaaaat catgttttat gtttaaatga
     1621 atcgattcta ttagtgcttt catgtataaa aaaggtgatt gtatataatg gtgtttaatc
     1681 atatcccttg cgtcatagac aatcatcatg tctgattctg atggcacaat ttctataata
     1741 aatggataat tttgttcaat taaatatgag cgaagtgtta ataaatcttc ggttaaactt
     1801 ttagatactt ctttttcaat tgcgactaca atggcttggt caccttgact atatattttc
     1861 atttgtacac ctcttcaata tttggttact ttactttcat ttttaattaa tagaatcgat
     1921 tgaatagttc attttatcta ctcaactaaa tttactgatg atacattaaa tccattgctc
     1981 gcacaacaaa aggcatgagt tcgtgaatta atgcatatac aaggataaag cttataacag
     2041 tagtaattgt tgctatcaaa cgaacaacat atattctatt ttcagatagc aatcgattca
     2101 ctaatcgata atttacgtat acaagaatta acaacagcac aatataaaca ataatcatat
     2161 ttggccccat ttaattttta aatttgtttt cacttcgttt tctacattat tatcatacta
     2221 attttaaagg caaaggtgga catcggcaac ctctcgtaaa ctatttatca aaaataaacg
     2281 tatctcattg ttatgatatt tataaatcaa ttcgttttta ttaaagtctt tttcaacaag
     2341 tttgtcacta tctattaaat aatcacgcat gcatcctttt aagaaatcat ctttataact
     2401 tggtgtgtac cattttccat cctcttcaat gacaatgttg ccaatatcaa attcaaggac
     2461 cttgccgtcc tctgaagtta gcaatattaa atctgtttca tgattgtgtg ctaaatgctt
     2521 tcgttctgtc gttttattat ttataaaagt tggattaact gctttgggca acacttgaaa
     2581 ttttgctgta aaactacttt tctcaggtaa aggtgacact atcgtttcaa attcgctttc
     2641 agcatttaat acgattttaa gtctatattg gccactgtga tactttgttg ttacatcgtt
     2701 taattcattt cgccatgcat gttcatcaaa tttaaagttt aatcgctcag aagaacattt
     2761 tatgcgatta gtatgataag taagtctagg gatatgtcca ttatcaattt tcattgtttc
     2821 aaataattgc attataacat ctccaaaatc ttggtttttg cgtaaaattc atttacttca
     2881 tcttttggct tagaatcaat tgtaatacct gctccgactc cataaatcgc ttgtccatat
     2941 ttatactcaa tagtacgaat tggaatatta aaaatcattt tatcatcttc agttggaagt
     3001 aatagtccaa ttgctccgca gtatatacca cgaggtgaac tttctaattg tttaatatat
     3061 ttcattgtat tcagtttcgg tgcacctgtt atcgaaccac aaggaaataa cgatgttaaa
     3121 atttgagtta agtctgtatt attttttaaa gttccactta ccatcgaagt catttgaaat
     3181 acagttttat atgcctcaat aaaaaatagt ttatacacct taattgagcc actctgtgat
     3241 attctcccta tatcgtttct tagtaaatca acaatcatga cattttctgc acgatctttc
     3301 gaagaagttt gcaatgtttt ataatactgt tgatcttcag cttccgtttt tcctctaggc
     3361 attgtccctt tcatcggttt gcttatgata acgttatcga cattgttaaa ttgtcctttt
     3421 tgaaaaaata attctggtga gatagacgct acttggattt catctgtttg taataacgca
     3481 gtataattac cattactaaa ttgagttaat cgttcatata aagtactaat aggataataa
     3541 atgttatctg ttaagcgcgc cgtatagttc acttgatacg tttcgccttc gacaattgct
     3601 tgttggacac gtttaatatt agtcatcata acctcagaag attcaacaaa tgaaaaatga
     3661 tgctttgata cataagaagt ttgatgttca tatgttgaat ttatgctttc cgctttttca
     3721 aaactataag ctactgcata aatatcatct ttagctaatg aatgtgtaca cataacatga
     3781 ttaaaatact ttgccgcttc gtaacttaaa tataacgaga catatctacc ttgtcgttgc
     3841 tgcgcttgtg caaagtgtat cacttctccc acatcagcca acttagtagc aacatacttc
     3901 tttataaatc cctttaattg aatatggtat tgcttatatt cattttcagt taaatagtag
     3961 cgataattat attctattct cacagtagtc acctaccttc gataaaaata attcaacttg
     4021 tcgataaccg tattcactca aaatagattc aggatgatat tgcacaccaa aaaccggaaa
     4081 tctaatatgc tcaaatgcca taataatcgc tgcatcgttt tttgctgtaa tctttaagca
     4141 atttggaaaa gtcgctccgt cagcaattaa tgaatgataa cgcattacat tgaaattttg
     4201 aggcagtcct tgaaaaatac cttcattggt atggcgtaac tgtgtagtat gtccgtgtac
     4261 aggatgatag ccgtgaatga tatttccacc aaaataagac acgatacatt gaaatcctaa
     4321 acatacacct agtataggta cacgctgata aaattgttct aacacttcat tcaagatagg
     4381 ataatcatcc ggattacccg gcccaggtga aataacaatt gcttttggct tcatattaat
     4441 gacgtcttct atcagcagat tatcaatacc aacaacttga actgttagtt tcgtttgagt
     4501 cttaatatag tctattaaat tatatgtaaa tgaatcatta ttatctatga ctagaatcat
     4561 tgtatactcc gttctaaatg tgttttattt ttataatatg tattggatgt agctaaaact
     4621 ttaaaagcat tgtcattatc ctgaccttga ttttaactaa tatatggtat attctattca
     4681 tcgtacataa atgaatatca gaggttccta gctgaaaccc tctataaaaa actagacatt
     4741 gaaatttcaa acattcgttg gtaaaccttt aatatatatt ataggagcaa cccatt
//
LOCUS       DAHHOJ010000145         4585 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.146, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000145 DAHHOJ010000000
VERSION     DAHHOJ010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4585
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.146"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..514)
                     /locus_tag="NBH32_002435"
     CDS             complement(<1..514)
                     /locus_tag="NBH32_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCD1516003.1"
                     /translation="MKLQWINTFKLHSKKRQLSKAQQIDLLLNLCNLLKYGFTLYQSF
                     QFLNLQMTYKNKQLGTTILSEISNGAPCNQILSLIGYSDTIVMQVYLAERFGNIIDVL
                     EETVNYMKVNRKSEQRLLKTLQYPLILVSIFIAMIIILNLTVIPQFQQLYTSMNIQLS
                     SFQKTLSFFIT"
     gene            complement(486..1460)
                     /locus_tag="NBH32_002436"
     CDS             complement(486..1460)
                     /locus_tag="NBH32_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II/IV secretion system protein"
                     /protein_id="HCD1516004.1"
                     /translation="MKILFQEIINKAIEMKASDVHFIPVKNEVSIKFRINDNLEQYEQ
                     IGNGIYQKLLVYMKFQAGLDVSTQQVAQSGRYSYHFNKIYFLRISTLPLSLGQESCVI
                     RIVPQFFQQQKSTYKFNDFKHLMNKKQGLLLFSGPTGSGKSTLMYQMVSYANKALNLN
                     VISIEDPVEMQIPGIVQINVNDKAGINYVNSFKAILRCDPDVILIGEIRDKDVAKCVI
                     QASLSGHLVLTTLHATDCKGAILRLLEMGVSVQELIQATNLIINQRLVTTIKQQRQLV
                     CEILSQQQLRYFFSHNHSLPSSFKNLEDKLDDMTKAGVICETTMDKYI"
     gene            complement(1512..2135)
                     /locus_tag="NBH32_002437"
     CDS             complement(1512..2135)
                     /locus_tag="NBH32_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1516005.1"
                     /translation="MRISSLTLGLVDTNTYFIENDKAVILIDPSGESEKIIKKLNQIN
                     KPLKAILLTHAHFDHIGAVDDIVDRFDVPVYMHEAEFDFLKDPVKNGADKFKQYGLPI
                     ITSKVTPEKLNEGSTEIEGFKFNVLHTPGHSPGSLTYVFDEFAVVGDTLFNNGIGRTD
                     LYKGDYETLVDSILDKIFELEGDLPLFPGHGPYTTVDDEQLNPFLHG"
     gene            complement(2132..2461)
                     /locus_tag="NBH32_002438"
     CDS             complement(2132..2461)
                     /locus_tag="NBH32_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MTH1187 family thiamine-binding protein"
                     /protein_id="HCD1516006.1"
                     /translation="MAIVDVVVIPVGTEGPSVSKYIADIQKKLQEYKAMGKIDFQLTP
                     MNTLIEGELSDVLEVVQVIHELPFDKGLSRVCTNIRIDDRRDKSRKMNDKLTSVQKHL
                     ENSGENL"
     gene            complement(2461..3447)
                     /locus_tag="NBH32_002439"
     CDS             complement(2461..3447)
                     /locus_tag="NBH32_002439"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HCD1516007.1"
                     /translation="MTKIILAADVGGTTCKLGIFTPELEQLHKWSIHTDTSDSTGYTL
                     LKGIYDSFVEKVNENNYNFSNVLGVGIGVPGPVDFEKGTVNGAVNLYWPEKVNVREIF
                     EQFVDCPVYVDNDANIAALGEKHKGAGEGADDVVAITLGTGLGGGIISNGEIVHGHNG
                     SGAEIGHFRADFDQRFKCNCGRSGCIETVASATGVVNLVNFYYPKLTFRSSILELIKE
                     NKVTAKAVFDAAKAGDQFCIFITEKVANYIGYLCSIISVTSNPKYIVLGGGMSTAGPI
                     LIENIKTEYHNLTFTPAQFETEIVQAKLGNDAGITGAAGLIKTYVLDKEGVK"
     gene            complement(3444..3647)
                     /locus_tag="NBH32_002440"
     CDS             complement(3444..3647)
                     /locus_tag="NBH32_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HCD1516008.1"
                     /translation="MSRKINNFYDVQQLLKSYGFLIYFKNPEDMYEMIQQEISSLYQY
                     ELLSKEEYLKCTLIINQRRNEQK"
     gene            complement(3628..>4585)
                     /locus_tag="NBH32_002441"
     CDS             complement(3628..>4585)
                     /locus_tag="NBH32_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1516009.1"
                     /translation="IWLCMILYLNNFSDVKLLDVGGLVHFNVVHGEWYRIVTSMFLHF
                     SFEHILMNMLSLFIFGKIVEAIIGSWRMLTVYFIAGLFGNFVSLSFNTTTISVGASGA
                     IFGLIGSIFAMMYVSKTFNKKMLGQLLIALVILVGVSLFMSNINIVAHIGGFIGGLLI
                     TLIGYYYKVNRNIFWILLIGMLVIFIALQIRIFTIKEDNIYNKLIKDDMTSGNYDNAQ
                     NIVKQTINKNYADDQTYYLSGMIMATINSKSEGMTEWERGLRMFPKSGLLNFELAIAN
                     RSLNDDEKALKYVRKALNTDPKNTDYINLEKELTKSNESKNK"
BASE COUNT         1535 a          839 c          584 g         1627 t
ORIGIN      
        1 tggtaatgaa aaaagacaat gttttttgaa aagaagatag ttgaatattc atagaagtat
       61 ataattgttg aaactgtgga attactgtga ggtttaatat aataatcata gcaataaaaa
      121 tcgaaactag tattaagggg tattgcagtg tctttaacaa tcgttgttct gactttctat
      181 tcactttcat ataatttacg gtttcttcta gaacgtctat gatattgcca aatctttctg
      241 ccaaatatac ttgcatgacg atagtatcgc tataacctat cagtgataat atctggttgc
      301 atggtgcacc atttgaaatt tcacttagaa tggtggtgcc taattgctta tttttatatg
      361 tcatttgaag atttaaaaat tgaaaacttt gatacagagt gaaaccatat ttcaacaaat
      421 tacataaatt taaaagtaaa tcgatttgtt gggccttact taattgtcgc ttcttagaat
      481 gtagtttaaa tgtatttatc cattgtagtt tcacaaatga cacctgcttt tgtcatatca
      541 tcaagtttat cttctaagtt cttaaatgat gatggtaatg aatgattatg ggaaaagaaa
      601 tatcggagtt gttgctgaga tagaatttca catactaatt gtcgctgttg cttaatagta
      661 gttacaagtc gttggtttat aattaagtta gttgcctgta tcaattcttg tacagaaaca
      721 cccatttcta atagccttaa aatagcacct ttacaatcag ttgcatgcaa tgtagtcaga
      781 acaaggtgac cacttaaact agcctgtata acacacttgg caacatcttt atctctgatt
      841 tcacctatta aaataacatc gggatcacat cttaaaatag ctttaaacga atttacatag
      901 ttaatgccag ctttatcatt cacattaatt tgaacaatac caggaatttg catctctaca
      961 ggatcctcta tagaaattac atttaaattc aaggctttat tcgcgtatga gaccatttga
     1021 tacattaatg tactctttcc tgaaccagtt ggtccactaa acaatagtaa tccttgtttc
     1081 ttgttcatga ggtgtttaaa atcattgaat ttataagttg atttctgttg ttgaaaaaat
     1141 tgaggtacaa ttctgataac acaactttct tggccaagtg acaatggtaa tgttgatatt
     1201 ctcaaaaaat aaattttatt gaaatggtaa ctatatcgac cgctctgtgc gacttgctgt
     1261 gtagaaacat caagcccagc ttgaaacttc atataaacta ataacttttg ataaatgcca
     1321 ttcccaattt gttcatactg ctccaagtta tcattaattc taaatttaat acttacttca
     1381 tttttaactg gaataaaatg tacatcactc gctttcattt ctatcgcttt attaattatt
     1441 tcttgaaata gaatcttcaa aaaaacacct cctacatata atcacgtagg aggtgttttt
     1501 attacttcaa tttaaccatg taaaaatgga tttaattgtt catcatcaac cgtcgtatat
     1561 ggaccatgtc cagggaataa aggtaaatcg ccttctaatt caaatatttt atctagaata
     1621 gaatcaacta gagtttcata atcaccttta tataaatctg tacgtccaat tccattatta
     1681 aataatgtat ctccaacaac tgcgaattca tcgaacacat atgttaaact tcctggtgaa
     1741 tgtccaggtg tgtgtaacac attaaactta aatccttcta tttctgtgct accttcgttt
     1801 aacttttcag gagttacctt acttgtaata attggtaatc catattgctt aaatttatct
     1861 gccccatttt taacgggatc ttttagaaaa tcaaactctg cttcatgcat ataaaccggg
     1921 acatcgaatc gatcaactat atcatcgact gctccgatat gatcaaagtg tgcatgtgtt
     1981 aataaaatag cttttaacgg tttatttatt tggtttaatt ttttaataat tttttcactt
     2041 tcacctgaag ggtcaatcag aataacagct ttgtcatttt cgatgaaata cgtattagta
     2101 tcaactaagc ctaaagttaa gcttgaaatc ctcataggtt ttcaccacta ttttctaaat
     2161 gtttttgtac tgatgttagt ttatcattca tttttctaga tttgtctcgt cggtcatcaa
     2221 tacggatatt tgtacaaact ctacttaaac ctttatcaaa aggtaattca tgtatcactt
     2281 gcacaacttc taatacatcg cttaattcac cttcaattag agtattcatt ggtgttaatt
     2341 gaaagtcaat tttacccatc gctttatatt cttgaagttt tttctgaata tctgcaatat
     2401 atttactaac actcggacct tccgttccaa ctggaataac aaccacatca acaatagcca
     2461 ttattttacc ccctctttat ctaatacata ggtcttgatt aatcctgctg ctcctgtaat
     2521 acctgcatca ttacctaatt tcgcttgaac aatttcagtt tcaaattgag caggtgtaaa
     2581 tgttaaatta tgatattctg ttttaatatt ttcaattaaa ataggtcctg cagtagacat
     2641 tcctccacct aaaacgatat atttcggatt acttgtaaca ctaataatac tacataaata
     2701 tccaatatag tttgcaacct tttcagtaat gaaaatacag aattggtcac cagcttttgc
     2761 cgcatcaaaa acagcttttg ctgtaacctt attttcttta atcaattcta atatagaaga
     2821 tctaaacgtc aacttcggat agtagaagtt aactaagtta acaacgcctg tcgctgaagc
     2881 aactgtttca atacatccag aacgaccaca attacattta aatcgttgat cgaagtctgc
     2941 tctaaaatga cctatttctg cgccagagcc attatgacca tgtacgattt caccatttga
     3001 aataattcct ccacctagac ctgtaccaag tgtgatggca acaacatcat cggcaccttc
     3061 accagcacct ttgtgtttct cccctaaagc agctatgtta gcatcattat ctacatacac
     3121 tggacaatca acgaattgtt caaaaatctc acgtacatta actttttctg gccaatataa
     3181 gtttactgct ccatttactg tacctttttc aaagtcaaca ggacctggaa caccaatacc
     3241 tacgccaagt acatttgaaa aattataatt attttcattt actttttcaa caaacgaatc
     3301 ataaattcct ttcaaaagtg tatatcctgt actatcagat gtatcagtgt gaatagacca
     3361 tttatgtaat tgttctaatt caggtgtgaa aatacctaat ttacaagtcg tcccgcctac
     3421 atcagctgct aaaataattt ttgtcatttc tgttcattcc ttctctgatt aattatcaac
     3481 gtacatttca aatattcttc cttagataac agttcatatt gatacaatga tgaaatctcc
     3541 tgttgaatca tttcgtacat atcttctgga tttttaaaat atattagaaa accgtaactt
     3601 ttcaataact gttgtacatc ataaaagtta tttatttttc gactcatttg atttagtcaa
     3661 ctctttttct aagttaatat aatctgtatt tttagggtct gtatttaatg ctttacgcac
     3721 atattttaat gctttttcat catcatttaa tgaacgattt gctatcgcta actcaaaatt
     3781 taataaacct gatttaggaa acattctaag tcctcgctcc cattctgtca taccttcaga
     3841 cttagaattg atagtagcca taatcatacc acttaaataa tatgtttgat catcggcata
     3901 atttttattt attgtctgct tcacaatatt ttgagcatta tcataattac cactagtcat
     3961 atcatctttg atcaatttat tataaatatt atcttcttta attgtaaaaa ttctaatttg
     4021 aagtgcaata aatataacaa gcataccaat tagtaaaatc caaaaaatat tacgattcac
     4081 tttatagtaa tagccaatta aagttattaa taaaccacca atgaatcctc caatatgcgc
     4141 cacaatattt atatttgaca taaacagaga aacaccaact aatatcacta atgcaattaa
     4201 taactgccct aacatttttt tgttaaatgt ttttgaaaca tacatcatcg caaaaattga
     4261 tccaatcaga ccaaatatag caccactagc cccaactgaa attgtagtcg tattaaatga
     4321 tagtgataca aagtttccaa acaaccctgc aataaagtat acagttaaca tccgccatga
     4381 accaataatt gcttcgacta ttttaccaaa aataaataat gaaagcatat tcataagtat
     4441 atgttcaaaa ctaaaatgta aaaacatcga tgtaacaatt cgataccatt caccatgtac
     4501 gacattaaaa tgcactaacc cgccaacatc taataatttt acatccgaaa aattatttaa
     4561 atataaaatc atacataacc atatt
//
LOCUS       DAHHOJ010000146         4486 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.147, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000146 DAHHOJ010000000
VERSION     DAHHOJ010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4486)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4486)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4486
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.147"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..332)
                     /gene="deoC"
                     /locus_tag="NBH32_002442"
     CDS             complement(<1..332)
                     /gene="deoC"
                     /locus_tag="NBH32_002442"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCD1516010.1"
                     /translation="MKFEKYIDHTLLKSESTRTQIDQIIDEAKAYNFKSVCVNPTHVK
                     YVAERLADSEVLVCTVIGFPLGASTTATKTFETEDAIQNGADEIDMVINIGALKDGRF
                     DDVQQDIEA"
     gene            complement(413..1765)
                     /gene="tet(38)"
                     /locus_tag="NBH32_002443"
     CDS             complement(413..1765)
                     /gene="tet(38)"
                     /locus_tag="NBH32_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD1516011.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            complement(1772..2479)
                     /gene="deoD"
                     /locus_tag="NBH32_002444"
     CDS             complement(1772..2479)
                     /gene="deoD"
                     /locus_tag="NBH32_002444"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1516012.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2715..3470
                     /locus_tag="NBH32_002445"
     CDS             2715..3470
                     /locus_tag="NBH32_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1516013.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLDRV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            complement(3813..4412)
                     /locus_tag="NBH32_002446"
     CDS             complement(3813..4412)
                     /locus_tag="NBH32_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1516014.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
BASE COUNT         1493 a          812 c          683 g         1498 t
ORIGIN      
        1 gcttcaatgt cttgttgtac atcatcaaaa cgcccatctt ttaatgcgcc gatgttgatg
       61 accatgtcaa tttcatctgc accattttga attgcatctt ctgtttcaaa tgttttcgtt
      121 gcagttgtcg acgcacctaa tgggaatcct attaccgtac aaactagcac ctctgaatca
      181 gctagtcgct ctgctacata tttaacatgc gttggattca cacatacaga tttaaaattg
      241 tatgctttcg cttcatcgat gatttgatcg atttgcgtac gtgttgactc agacttcaat
      301 aaagtgtgat ctatatattt ctcaaatttc atacttacta ctcctcgtgt tatataatct
      361 ctttatttaa ttttactata aatacgaata tatctcgcga atttatatct atttattttt
      421 cagattgtgt ccaacgattt aagatgacga ataaaactaa tccgacaata ctcattaaac
      481 ctaaaataat taaaataata ccgaaatgtg aacttaatgc atcattgcct gggaaattta
      541 atgctttaaa atcgattaga gccgcagcaa tcgcaatacc tacagatacc gccacattaa
      601 taattaaatt ataaaaacca atagccacac ctgtcatatt aagatctatt gttttaatgg
      661 cttcgttaag taaaggtgca tacattaaag caaagctacc tgcaaagaat atcatagaaa
      721 tgacgaagat tgaaatgtga ttacctactg caaatgcagg taaaattaag ctcagtgcga
      781 ttaaaataat tgctgtgata atcgcttgtt ttgaattaag atattcgccg attttaccac
      841 ttagtgcacc aacgatgact gctacaatat aacccggtac taataacagt gatgttgtgt
      901 ctagttgaag atgataaatt tgttccatta tgaatgggaa cgtaaaaata taacccaatt
      961 ggatagcata cattacgaat actataaata aaaatgaagc ataacgttta ttttggaaaa
     1021 atgatttgtt tactaatgga cgttgcgcat ttttaatata tagcgcaaaa acgataatcg
     1081 caattaaagc accaatcata tataaccaat taaagttcgt aataaacagc atgactgttg
     1141 tagcaatagt tgccactaga attaagccaa caaaatctaa atgagcttta tttgtattat
     1201 tttcttttgg taataattta aataggaatg gcaacgtaaa tactacgatt aatgcaatta
     1261 aaaacatatt tgtccagtgt aaatacgtag aaataaatcc acctgataat gtaccgataa
     1321 ctaatgacaa ggaataactg ctcgtactta agccaaggta agtcttctgg tcctctttag
     1381 aaagatactt tgcaacatat atcacatata atgtctctgc agcagctaaa cctgcagttt
     1441 gaataatacg tccaactaaa agtaatgcga attgatgttg gaaaatgtaa ccaataattg
     1501 atccgataat gattaatatc acgccataaa taaatagtgt gcgtatacta atggcatcag
     1561 ataatgaagc gtatactaca gcgccaatac caattactaa accggctaat gtcgcttgcc
     1621 aacttactgt agttacagaa atgtttaagt catcagcaat ggctacagaa atcaatttga
     1681 atgagttgtc tataaccaaa ctgaatacaa ataagaataa taaaataggt actgctttct
     1741 ttattttaga atattcaaca ttcataatca tctacaccaa tgacagtgca atttcaatca
     1801 tatctgtaaa tgcacgttcc ctttcctcag gtgttgttga cgtttcatga attaaatgat
     1861 cgctcactgt gaacacacct aatgcttcga caccagcgta aattgcattc atgtataatg
     1921 cagctgattc catttctaca cctaaaatac ccatacgcat ccaacgttca ctcgctgttg
     1981 tatccgcgtt atagaaaata tcacttgata acacattacc tacatgatga cgtacacctt
     2041 tgtcacgtgc tgtttcaact gctttttcta ataactgata agaagcaatt ggcgcaaaat
     2101 gacctggtaa ttgatattgt tgaacgtaat ttgaatcagt agaggcacct tgtgcaataa
     2161 tcacatcata taaatcaatg ttttcttgca tcgcgccaca agaaccaacg cgaattaatt
     2221 ttttacaacc aaatgtatga attaattcat aagagtaaat gccaatagat ggcataccca
     2281 tacctgaacc catgacagaa acttttttac ctttatatgt tccggtaaaa ccaaacatgt
     2341 ttcgcactgt attgaactgt tccacatcgt ccaaatatgt ttctgcaatg aacttagctc
     2401 ttaacggatc tcctggcaat agcaccgttt ctgcaatttc gacgtcattc attggcttaa
     2461 tgtgtggtgt tgatttcata aaatattcct cccaatattt gtatagtgat ttgtctattt
     2521 gtctaaccat gtatataata atatttttta aaagcgcttt caagcattac attttgacaa
     2581 tattaagaac attttacagt tatagcttct aaccttgtat aaaactaatt ttcaaagcct
     2641 atctatgttt aatttttata atttttccaa ttcgagtaaa aattattgta tgatgagagt
     2701 gattaagtaa ctttatggag gttgtctacg tgacgcaaac acaaccaaaa tttttaacga
     2761 tttataatac gttatataaa gaaattcaaa taggaaaata tccgagtggc catgcattac
     2821 ctactgaaaa ggaactttgt gcgcgctttg atgtcagtcg tatgactttg cgccaagcta
     2881 taaaattgtt ggccgaagat ggcatcgtag agagtacaag agggaaggga cacttcgtta
     2941 ttccacaaac aaatgcccaa cacgcttcaa gtatggatca atttaagcat ccacttgatc
     3001 aaatattgct agatcgtgta tctatgtcat caattaatta tcgtgttgat ttagaaagtg
     3061 aatatacaaa tcatttgttt gcaaaccatc cttctgccgt cattgcaatg gaacgctatt
     3121 atcaaaaaaa ggacaatcat tctaagcaag ctgatgcatt ttgttttacc tttataccgt
     3181 taaatgtcat tgacacattt aaagtgaata cacaaaacga agacgacatg aagacgttcg
     3241 ttgagcagac gatttatagc aatgcatatc aatcagattt aaaaatgtct attaccaaag
     3301 cgccccactt caaaaatcat agtcacgtgt ttgatggtga tacacattgt tggctgatta
     3361 ttgaaacatt gtacgcacaa acaccttatc caattatgat taataagtgg tatattccac
     3421 aggaaatatc tgaattaaca ctgacacgta taagacaatc ggattattaa aattattaaa
     3481 ataaaaaatc atgtgatgac tgtttagcta ttgtttaatc gacaataact ttcaggtaat
     3541 cacatgattt tttctacttt aaaacgtatg atgtttattt atttgttgaa cgttgtctaa
     3601 ataattttgt tattcgatgt gcgctcttct cgaaacattg agttcacaag cacagagaac
     3661 acaagtaaag tattcatcat tgtaaatgat tgtactgttt tacctacatt tgattctcca
     3721 acccattata caaaaagcaa attgattacc tcatacatac aaaaaaggtg aaacaccttg
     3781 tagatgctcc acctcaatta tattaagtta tattattttg ctgcttggta taattcatca
     3841 acttttttcc agttaacaat attccaaaat gcagtcatat agtctggacg tttattttga
     3901 tatttcagat agtaggcatg ctcccaaaca tcaaatagta agattggtgt tttgccttct
     3961 gttaatggat tatcttggtt tggcgttgtc acaatttcta attttccatc attaacaact
     4021 aaccaagtcc aacctgatcc aaataatgtt gttgctttat ttgcaaattc atttttaaat
     4081 tcatctaaag tgccccactg cgctttaatg tcgtctatta cgccaccttt ttcttcagaa
     4141 ttaggtgata gtatttccca gaataatgaa tggttaaaat gaccaccgcc attattacgg
     4201 actgacatcc tcatcgcttc cggtaccttg tctaagttag caatcatatc cgctagtgat
     4261 tgatgctcta actctgttcc ttcaactgtt gcgtttaatt tcgtcacgta cgtattgtga
     4321 tgtttgtcgt gatgaaactc cattgttctt tgatctatat atggttccaa tgcatcatat
     4381 gcatatggta aatttggtaa tttaaatgcc ataagtatat tcctcctttt atgaatatac
     4441 ttttataata attaatttcg ggtgtgtttt gcaataatta ttattt
//
LOCUS       DAHHOJ010000147         4469 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.148, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000147 DAHHOJ010000000
VERSION     DAHHOJ010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4469
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.148"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            170..1351
                     /locus_tag="NBH32_002447"
     CDS             170..1351
                     /locus_tag="NBH32_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000162901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl transferase"
                     /protein_id="HCD1516015.1"
                     /translation="MTKKNKSILISKLLFFTENNKLIEENLRIADLFYEHDIALGILA
                     RSTTINILRSKIPTDYQEKINFLSRDDKNKKDYIKQNKDLIIAVIGVVNEDAIYSFNC
                     RLPLFNPEKLISKELDISEKVKQYGLPINKFENVIDCLKAYEIHQENYFNIPFNQKFS
                     VISLNNANTYQSPKDEERIKNIFSANLKGSVSERDQEILLILLFHLINEVRTNSYYDS
                     VKYWGTFPSSDPENKNTSISFLKECVRKILNGLPKKGSELLIRQKEMQSKHTTRSTER
                     INIKSNKDFETLIVNPELVNKIEGEVVCIIDDYITNGYSAEAAKHLLLAAGAKEVIFL
                     AFGKFGTKYYSTNYKIKGDVSSNYTYEFESELLCERVYEGKQVYNNGNNSEISRFNEL
                     I"
     gene            complement(1668..1967)
                     /locus_tag="NBH32_002448"
     CDS             complement(1668..1967)
                     /locus_tag="NBH32_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000095392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WXG100 family type VII secretion target"
                     /protein_id="HCD1516016.1"
                     /translation="MSSKDAKKSQEEARLQQLKQEMRNSTDNMVSNAKNNVALKSQTL
                     NEIIETINNSSQNLEGTFEGKASEAAQQSINKLKSNNENLKQKFETLLNTMKING"
     gene            complement(1982..3595)
                     /locus_tag="NBH32_002449"
     CDS             complement(1982..3595)
                     /locus_tag="NBH32_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001836790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipase"
                     /protein_id="HCD1516017.1"
                     /translation="MLLRDSDARMLSENSYDKLSVDEVYTFSNKKQYKVIEKRDDTQN
                     GFRAYVFAPVIKGKVDKSQLLIGYAGTDPYSLNDIRTDLQLPIHHNTNNLKINNHYKD
                     LKNSTIHKKYNTIKDKNDLFSFSSNLNSKALFGDLWAAKFAPTQMDESDAFTESVKKK
                     YPDSEIHPLGHSLGAFLAQYNLIKHNLDNGTTFAAPNVYHSFTGDFKKDIDNGVYDSK
                     IRNIGHFDDPINNFNFFDHPIGENIIVMPHYDGFITALPLIGQHTIESYNDFDADGTV
                     KEMPELEQFNHDPFLNNRFQLFDPFGINSRFFDSFKIDSSILNRPSENDFDPFAILNK
                     KDKLFESNDLDIEGFNNKPKEDLSIDLNPFDDELNDEDEPLIPKRGDSKKLKAEKKIS
                     VKKGNGGSKNTGKKVKIQPDEVRNIANTIRDRIFLYSQIITALDEYREETISGANRIL
                     DKYQSDLLSGSHEFTTPYDHEQYMEILSVEGSFGNFKFYNSGLMDDLIQELDQNKKDL
                     LQFAEKLEFAANKFEEKDLEESDVFGLFS"
     gene            3640..4080
                     /locus_tag="NBH32_002450"
     CDS             3640..4080
                     /locus_tag="NBH32_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000759947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1516018.1"
                     /translation="MKKYIKLLIIVIITCLIIVGYLLHKSKKEHYIETQEKRIDLYFK
                     YNLNNYHSMKVTSFKKNPMGGYFIKGFVNNKKNYKFDAVIFSDTNKQFKGDLGYKEDE
                     IGELFREKNANDRLTVDEIIEKEHLDKSEYEAEPPLFFFSGPLE"
     gene            4286..>4469
                     /locus_tag="NBH32_002451"
     CDS             4286..>4469
                     /locus_tag="NBH32_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001036448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1433 domain-containing protein"
                     /protein_id="HCD1516019.1"
                     /translation="MNKKPLVIVFIIIILILGGIFLKMKYDEMKYYDEQKERIILFMK
                     YNVKGFKEIQFEEQKEN"
BASE COUNT         1627 a          563 c          672 g         1607 t
ORIGIN      
        1 tatatagcaa atattagcga tatagtggta gtaactgaaa cgaatttaaa aagtggaact
       61 atgaatacaa tcagaagtgc gagtatagca gggaaaaaga tcttgttcgt aaatcaagga
      121 gataatctaa ttaataacaa aatttatgaa tttggtgggg aaatgataga tgactaaaaa
      181 aaacaaaagc attttaatat caaagttatt attttttaca gaaaacaata aattaattga
      241 agaaaattta agaattgcag atctgtttta tgaacatgat atagcattgg ggattttagc
      301 tcgatctaca acaataaata tattacgctc caaaattcca acagattatc aagagaaaat
      361 aaattttttg agtagagatg ataaaaataa aaaagattac ataaagcaaa acaaagattt
      421 aattattgct gtaataggag tagttaatga agatgctata tactctttta actgtagatt
      481 accgttattt aatccagaaa aattaattag taaagaattg gatatatctg aaaaagtaaa
      541 acaatatggt ttgcctatca ataagttcga aaatgttatt gattgtttaa aggcatatga
      601 aattcatcaa gaaaattatt ttaatatacc atttaatcaa aaatttagtg taataagttt
      661 gaataatgca aatacatatc aaagtccgaa ggatgaagaa aggataaaga atattttttc
      721 tgcaaattta aaaggtagcg tatctgagag ggatcaagaa attttattaa ttttactttt
      781 tcatttgatt aacgaggtaa gaacaaatag ttattatgat agtgttaagt attggggtac
      841 atttccttct tctgatcccg aaaataaaaa tacatctatt tcatttttaa aggaatgcgt
      901 taggaaaatt cttaatggtt tgccaaaaaa gggatcagaa ttgctaatca gacaaaaaga
      961 aatgcaatca aaacatacta ctagaagtac agaacgtatt aatattaaaa gcaacaaaga
     1021 ttttgaaact ctaatagtga atccagaact agttaataaa attgaaggag aggtagtttg
     1081 tattatagat gattacataa ctaatgggta ttcagcggag gcagctaaac atttgctttt
     1141 agcagcagga gctaaagaag ttatttttct tgcatttggt aaattcggaa cgaaatatta
     1201 ctccacaaat tataaaataa aaggtgatgt atcaagtaat tatacatatg aatttgaaag
     1261 tgaattactt tgcgaaaggg tttatgaagg aaagcaagtt tataacaatg gtaataattc
     1321 tgaaatatca cgctttaatg aattaattta aatgaactta gtcacttatt agtgcagtgt
     1381 atcttcttag tatttataaa atgtcagtta atcccattta atatattatg ggtgctcgtt
     1441 tatacttgtg atgtattgcg tgttgaaata tatatagcat ctatagatat cgtaattaag
     1501 tataattttc acagaagtag aaattaaaga acaatttata tttacgtatt aagaattagg
     1561 aatgacctac ttgccgtcat gaaaattaaa atcccttcac tatttgttta gcgaggggat
     1621 tttcgtaatg tataaaaaaa tacagataaa ttaataattt ctcaatatta tccatttatc
     1681 ttcattgtat ttaatagggt ctcaaatttt tgtttaagat tttcattatt gcttttgagt
     1741 ttatttatac tttgttgggc tgcttcgctt gcttttcctt caaatgtacc ttctaaattt
     1801 tgcgaagaat tattaattgt ttcaatgatt tcattcaatg tttgagattt taatgctaca
     1861 ttatttttag cattgctaac catattatca gtactattac gcatttcttg ctttaattgt
     1921 tgtaatctag cttcttcttg acttttcttt gcatcttttg agctcatata aattaccctc
     1981 tttaactaaa tagtccaaaa acatcgcttt cttctaagtc tttttcttcg aatttatttg
     2041 cagcaaattc tagtttttca gcaaattgta gcaagtcttt tttgttttga tctaattcct
     2101 gaattaagtc atccattaaa ccactattat aaaacttgaa atttccaaaa cttccctcaa
     2161 ctgataaaat ctccatatac tgttcatgat cataaggtgt tgtaaattca tgtgaaccgc
     2221 ttagaaggtc cgattgatat ttatctaaaa tgcgattagc ccctgatatt gtttcttctc
     2281 tatattcatc caatgcagta attatttgac tatacaaaaa tattctatca cgaattgtgt
     2341 tggcgatatt tcgcacctca tcaggctgga tttttacttt cttacctgta tttttggaac
     2401 caccattacc ttttttaaca gagattttct tttctgcttt taatttttta ctatctcctc
     2461 ttttgggaat taatggttcg tcctcatcat ttaattcatc atcaaaagga tttaaatcaa
     2521 tgcttaaatc ttcttttggt ttattattaa atccttctat atcaaggtca tttgattcga
     2581 ataatttatc ttttttattg agaatagcaa aaggatcaaa atcattttca cttggtctat
     2641 ttaatataga actatcgatt ttgaaagagt cgaaaaatct gctatttata ccaaatgggt
     2701 caaataactg aaatctatta ttaagaaatg ggtcatgatt aaattgttca agttctggca
     2761 tttccttcac tgtaccatcc gcatcgaaat cattgtatga ttcaattgtg tgttgaccaa
     2821 ttaaaggaag agcagttata aatccatcat aatgtggcat tacaattata ttttcaccaa
     2881 ttggatgatc gaagaagtta aagttattaa taggatcatc aaaatggcca atattgcgta
     2941 tttttgaatc atatacacca ttatcaatgt cttttttaaa gtcgccagta aatgaatggt
     3001 atacgttagg tgctgcaaac gttgtaccgt tatccagatt atgttttatt aaattatatt
     3061 gagctaaaaa cgcacctaaa ctatgtccta atgggtgaat ttcactatct ggatatttct
     3121 ttttaactga ttcagtaaaa gcatcacttt cgtccatttg agtcggtgca aacttagctg
     3181 cccatagatc accgaaaagt gctttactat ttaaattaga agaaaaacta aaaaggtcat
     3241 ttttatcttt tattgtattg tattttttgt gaatagttga gttttttaaa tctttataat
     3301 gattattaat ttttagatta tttgtgttat gatgaatggg aagttgtaaa tcagtacgaa
     3361 tatcatttag actatatggg tcagtgccag cgtaacctat taaaagttga ctcttatcaa
     3421 ctttaccttt gattactgga gcgaaaacat atgctctaaa cccattctgg gtgtcgtcgc
     3481 gtttttcgat gactttgtat tgttttttat ttgaaaatgt atatacctca tcaactgata
     3541 acttatcata tgaattttcg gataacattc ttgcatcgct atctcttaat aacatgtgga
     3601 acactcctta taaaaaataa aaaagaggta ataataaaaa tgaaaaaata tatcaaacta
     3661 cttattatag taattataac atgtttaatt atagtagggt atttgttaca caaaagtaaa
     3721 aaagaacatt atattgagac acaagaaaag cgaatagatt tgtattttaa atataatctt
     3781 aacaattatc attcaatgaa agttacaagt tttaaaaaga atccaatggg gggttatttt
     3841 ataaaaggct ttgtcaataa taaaaaaaat tataaatttg atgctgtgat attttctgat
     3901 actaataaac aatttaaagg agatttaggt tataaagagg atgaaatagg tgaattattt
     3961 agagaaaaaa atgctaatga ccgattaact gtcgatgaaa taattgaaaa agaacacttg
     4021 gacaaaagtg aatatgaagc cgagccaccg ttatttttct tttcaggtcc acttgaataa
     4081 ataatatgaa tagattagat aatttaatga tttatatttt aaaggaaact aaatctatat
     4141 taaagaatat gtaaaactaa aatttactaa tgttaaaacc attagtcata tgggttgcaa
     4201 cgtttttaca ttcatcgtaa tactctgtat gcttcattaa tttaaaatgt tcgtcaataa
     4261 atccaatgaa aaggagatgg gttttttgaa taaaaaacct cttgtgattg tatttataat
     4321 tataatattg attctaggcg gaatcttttt gaagatgaaa tatgatgaaa tgaaatatta
     4381 tgatgagcaa aaagaaagaa tcatattatt tatgaaatat aatgttaaag ggtttaaaga
     4441 aatacaattc gaggaacaaa aagaaaacc
//
LOCUS       DAHHOJ010000148         4430 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.149, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000148 DAHHOJ010000000
VERSION     DAHHOJ010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.149"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            162..761
                     /locus_tag="NBH32_002452"
     CDS             162..761
                     /locus_tag="NBH32_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1516020.1"
                     /translation="MAFELPKLPYAFDALEPHFDKETMEIHHDRHHNTYVTKLNAAVE
                     GTDLESKSIEEIVANLDSVPANIQTAVRNNGGGHLNHSLFWELLSPNSEEKGTVVEKI
                     KEQWGSLEEFKKEFADKAAARFGSGWAWLVVNNGQLEIVTTPNQDNPLTEGKTPILGL
                     DVWEHAYYLKYQNKRPDYIGAFWNVVNWEKVDELYNATK"
     gene            882..2957
                     /locus_tag="NBH32_002453"
     CDS             882..2957
                     /locus_tag="NBH32_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HCD1516021.1"
                     /translation="MLKRLKEKSNDEIVQNTINKRINFIFGVIVFIFAVLVLRLGYLQ
                     IAQGSHYKQIIKNDENITVNESVPRGRILDRNGKVLVDNASKMAITYTRGRKTTQSEM
                     LDTAEKLSKLIKMDTKKITERDKKDFWIQLHPKKAKAMMTKEQAMLADGSIKQDQYDK
                     QLLSKIGKSQLDELSSKDLQVLAIFREMNAGTVLDPQMIKNEDVSEKEYAAVSQQLSK
                     LPGVNTSMDWDRKYPYGDTLRGIFGDVSTPAEGIPKELTEHYLSKGYSRNDRVGKSYL
                     EYQYEDVLRGKKKEMKYTTDKSGKVTSSEVLNPGARGQDLKLTIDIDLQKEVEALLDK
                     QIKKLRSQGAKDMDNAMMVVQNPKNGDILALAGKQINKSGKMTDYDIGTFTSQFAVGS
                     SVKGGTLLAGYQNKAIKVGETMVDEPLHFQGGLTKRSYFNKNGHVSINDKQALMHSSN
                     VYMFKTALKLAGDPYYSGMALPSDISSPAQKLRRGLNQVGLGVKTGIDLPNETKGQIE
                     PLTNNPGNYLDLSIGQYDTYTPLQLSQYVSTIANDGYRIQPHIGLTIHESTNKDEVGP
                     LKKKINGTVLNKVNNTENEIKQIQEGFKMAFNDKDGTGYVSFKDTVVPTAGKTGTAEV
                     FQNGEPRVNSTYIGYAPIDDPKLAFSIVYTNQPVPPPWLTGGDLGRDVINYYFKQLGK
                     DDKNKDKDK"
     gene            3070..3219
                     /gene="rpmG"
                     /locus_tag="NBH32_002454"
     CDS             3070..3219
                     /gene="rpmG"
                     /locus_tag="NBH32_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1516022.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERVEMKKFCSRENKQTL
                     HRETK"
     gene            3428..3967
                     /locus_tag="NBH32_002455"
     CDS             3428..3967
                     /locus_tag="NBH32_002455"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCD1516023.1"
                     /translation="MTKNEIRKYILHKMKNFNKAEKRKADTWLRNQFFATEEYKEANA
                     IALVLSFNHEVDTFSIIEQALMDHKRIFVPKMDYLNHQMTFKEIFNLKDIDVDNKGVY
                     YPTSKGETTNNLDLIVVPGVGFQDDGYRIGYGGGYYDRFLANYQTKTISLLYDFQITS
                     FEPESFDQPVDKLIIYQSA"
     gene            3979..>4430
                     /locus_tag="NBH32_002456"
     CDS             3979..>4430
                     /locus_tag="NBH32_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001652331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HCD1516024.1"
                     /translation="MNIDKQFWKTIYYWIRYLNFDIVSREKDDQEIWLAHKRKKQVVI
                     FKQHIKSTQEIRFDKAKVLEHKDEIANFISFEPQSFEFYYFTESEFSEEQLNEVSPIR
                     IKFNVIRHTKDLVKHMPNIFLARLISEDNDKKTYMFYKRKVLTDNFLD"
BASE COUNT         1729 a          601 c          792 g         1308 t
ORIGIN      
        1 tctgttaact ttttatatac aaaatgttta attatttttg atttgagtta tattaaatga
       61 gtagagaaaa taataagtag tagtatttaa gcaaattctt tgttgaacca gtcactgctt
      121 gttattattt acttacagac attttaggag gatgattatt tatggctttt gaattaccaa
      181 aattaccata cgcatttgat gcattagaac cacattttga caaagaaact atggaaattc
      241 accatgacag acatcataac acttatgtta cgaaattaaa tgctgcagta gaaggtacag
      301 atttagaatc taaatctatt gaagaaattg ttgctaattt agacagtgta ccagctaaca
      361 tccaaactgc tgtacgtaat aatggcggtg gacatttaaa ccattcatta ttctgggagt
      421 tactttcacc aaactcagaa gaaaaaggta ctgttgtaga aaaaattaaa gaacaatggg
      481 gttctttaga agaatttaaa aaagaatttg ctgacaaagc agctgcacgc tttggttcag
      541 gttgggcttg gttagtcgta aacaatggcc agttagaaat tgtgactaca ccaaaccaag
      601 ataatccatt aactgagggt aaaacaccta ttttaggttt agacgtatgg gaacacgctt
      661 attacctaaa atatcaaaac aaacgccctg actacattgg cgcattttgg aatgtagtta
      721 actgggaaaa agttgacgaa ttatataatg caacaaaata aagtatgatt acttgttcgg
      781 tctcttaaat gagaccgtta tttttttgtc aaaaagatag aaataatttc taaattcata
      841 tatgatttaa agtgaaagac tttgaataga ggtaggtagt tttgttaaaa agactaaaag
      901 aaaaatcaaa tgatgaaatc gttcaaaata caattaacaa gagaattaac tttatatttg
      961 gtgtgattgt atttattttt gcagtactag tactacgttt aggttattta caaatcgcac
     1021 aaggctcaca ttataaacaa attataaaaa atgatgaaaa cattacagtg aatgagtctg
     1081 tgccaagagg tcgtatttta gacagaaatg ggaaagtttt agttgataat gcttctaaaa
     1141 tggctattac atatactagg ggtcgaaaaa caacacaatc ggaaatgttg gatacggctg
     1201 aaaagttatc aaagctaatc aagatggata ctaagaagat tacagaacgt gataaaaaag
     1261 atttctggat tcagttgcat cctaaaaaag caaaagcaat gatgacaaaa gaacaagcta
     1321 tgttagcaga tggaagtatt aaacaagatc aatatgataa acaactgtta tcgaaaatcg
     1381 gaaaatcaca attagatgaa ttgtcttcta aagatttaca agttttagcc atttttcgag
     1441 agatgaatgc aggaacagtt ttagatccac aaatgataaa aaatgaagat gtcagtgaaa
     1501 aagagtatgc agcagtttct cagcaacttt ccaaattacc aggtgttaac acgtctatgg
     1561 attgggatag aaaatatcca tatggcgaca cattaagagg tatattcgga gatgtatcga
     1621 cacctgctga aggtattcca aaagaattga cagaacatta cttatccaaa ggatattcac
     1681 gcaatgatcg tgttggaaaa tcttacctag aatatcaata tgaagatgta ttgcgtggta
     1741 agaagaaaga aatgaaatac acaacggaca aatctggtaa agttacatct tcagaagtgt
     1801 taaatcctgg cgctcgcggt caagatttga aattaacgat cgatatagat cttcaaaaag
     1861 aagtagaagc attattagat aaacaaatta agaagcttcg cagccaaggt gcaaaagata
     1921 tggataatgc tatgatggtt gtacaaaatc ctaaaaatgg agacattctt gcgcttgccg
     1981 gaaagcagat taataagagt ggtaaaatga ctgattatga cattggtacg tttacttctc
     2041 aatttgcagt tggatcttct gtaaaaggtg gaacattatt agctggttat cagaataaag
     2101 ctatcaaagt tggagaaaca atggtcgatg aaccattaca tttccaaggt ggtttgacaa
     2161 aacgatcata ctttaataaa aacgggcatg tatctattaa tgataagcaa gctttgatgc
     2221 attcatcaaa cgtatatatg tttaaaacag cattaaaatt agcgggagac ccttattatt
     2281 ctggtatggc tttaccatca gacataagtt cacctgccca aaagctaaga agaggattga
     2341 atcaagtagg cttaggtgtg aaaacaggta tagatttacc aaatgaaaca aaaggtcaaa
     2401 tcgaaccatt aacaaataat ccaggtaatt atctagattt atcaattggt caatatgata
     2461 cctatacacc attacaatta tcacaatatg tttcaactat agcgaatgat ggttatagaa
     2521 tacaaccaca cattggatta acgattcatg aatcaactaa taaagatgag gttggtccac
     2581 tcaagaagaa aattaatggc accgtcttga acaaagttaa taatactgaa aatgaaatca
     2641 aacaaattca agaagggttc aaaatggcgt ttaatgataa agatggtact ggatatgtta
     2701 gttttaaaga tacagtagtg cctactgctg gtaaaactgg taccgctgaa gtgttccaaa
     2761 acggagagcc aagagttaac tctacttata taggatacgc gccaattgat gatccaaaat
     2821 tagcgttttc aattgtatat acaaatcagc ctgtaccacc accatggtta acaggtggag
     2881 acttaggtag agatgtaatt aactactact ttaagcagtt aggtaaagat gataaaaata
     2941 aagataaaga taaataaaat ttaacctgac gattgtgtag cgcatggttg taaaatttta
     3001 actttgcaaa tattatagat gttggtataa taataaagtc gtatttagaa atgataagga
     3061 ggaataacca tgcgcgtaaa cgtaacttta gcttgtacgg aatgtggtga cagaaactac
     3121 attacaacta agaacaaaag aaataatcca gaacgtgttg aaatgaagaa attctgttca
     3181 cgtgaaaaca aacaaacttt acaccgtgaa acaaaataat cattaaaatt aaatttaaat
     3241 taacaataaa aaaggtgaaa tctcaaaata agaggtttca cctttttaat tgtgcacacg
     3301 tatgaaatga taaaagattt tcaattgaaa taataaattt tagaattgtc gcataattca
     3361 tcatgacaac ataatgacat gtattgttat taacgatata atagaattga ataaagtggg
     3421 tgataatgtg actaaaaatg agattagaaa atacatttta cataaaatga agaattttaa
     3481 taaggctgaa aagcgaaaag cagacacatg gttaagaaat caattttttg caactgaaga
     3541 atataaagaa gcaaacgcaa ttgcgctagt tctttctttt aatcatgaag tagatacttt
     3601 ttctattatt gaacaagcct taatggatca taaacgtatt tttgtaccga aaatggatta
     3661 tttaaatcat caaatgactt ttaaagagat atttaatctc aaagatattg atgtcgataa
     3721 taagggggtt tactatccaa cttcaaaagg tgaaacaacg aataacctag atttaattgt
     3781 tgttcctggt gttggatttc aagacgatgg atatagaatt gggtatggtg gtggctatta
     3841 cgacaggttt ttagctaatt atcagacaaa gacaataagc ttattatacg attttcaaat
     3901 aacatcattt gaaccggaat cattcgatca accagtcgat aaattgatta tatatcaatc
     3961 agcatagtgg aggactaaat gaacatagac aaacaatttt ggaaaacaat atattattgg
     4021 attaggtact tgaattttga tattgtcagt agggaaaaag atgaccaaga aatttggtta
     4081 gcccataaaa gaaaaaaaca agtagtcatt tttaaacaac atatcaagtc tacccaagaa
     4141 atacgttttg acaaagcgaa agtgcttgaa cacaaagatg aaatagcaaa ttttatttct
     4201 ttcgaaccac aaagttttga attttattat tttacagaat cggaattttc agaagaacaa
     4261 ttaaatgaag tttcgccaat tagaattaaa ttcaatgtta taagacacac aaaagatttg
     4321 gtaaagcata tgccgaatat atttttggct agacttattt cagaagataa tgataaaaag
     4381 acatatatgt tctataaacg caaagtatta accgataact ttttagataa
//
LOCUS       DAHHOJ010000149         4422 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.150, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000149 DAHHOJ010000000
VERSION     DAHHOJ010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4422)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4422)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4422
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.150"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(451..2259)
                     /gene="pepF"
                     /locus_tag="NBH32_002457"
     CDS             complement(451..2259)
                     /gene="pepF"
                     /locus_tag="NBH32_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HCD1516025.1"
                     /translation="MSQQLSREEQERKYPEYTWDLTTIFKDDEAFEAAFKEVENELGK
                     EEQFKGHIGDSAETLYNALELEDTLGTKLEKVYVYAHLKQDQDTTNDKYTGMESRAHQ
                     LIIKFSSAWSFLVPEILQIDEDKIQSFVNSYDKLQKFAFDLKLINEKRPHILDAETEK
                     LLTEAQDALSTPSNVYGMFSNADLVFEDAIDKDGNAHPLTQGTFIKYLESDDRKLRES
                     AFRNVYKAYGAHNNTLGATLAGEVKKNVFNARTHNYKTAREKALSNNHIPENVYDNLV
                     KTVHKYLPLLHRYTELRKELLGLDDLKMYDLYTPLIKDIKFEMPYEEAKEWMLKALEP
                     MGEEYLNVVKEGLNNRWVDVYENKGKRSGGYSSGAHLTNPFILLNWSNTISDLYTLVH
                     EFGHSAHSYFSRKFQPSNSSDYTIFVAEVASTCNEALLSDYMDKHLDDEKRLLLLNQE
                     LERFRATLFRQTMFAEFEHKIHAIEEAGEPLTPTRMNEEYAKLNKLYFGDSVETDEDI
                     SKEWSRIPHFYMNYYVYQYATGYSAAQSLSHQILTEGKPAVDRYINEFLKKGSSNYPI
                     EILKNAGVDMTTPEPIEQACEVFEQKLNAFEKLMKA"
     gene            complement(2307..3293)
                     /locus_tag="NBH32_002458"
     CDS             complement(2307..3293)
                     /locus_tag="NBH32_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HCD1516026.1"
                     /translation="MLVALNEEKERVLATTALRKTQYFCPVCGKQVILKRGLKVISHF
                     AHKHLAEQKCFNNETIKHYKSKLILAQMIQQQGCKVEIEPFLKEIKQIPDILINNKYV
                     IELQYSPIPYKQILQRTEGLKKMGYKVSWLLNDVDYCHNKVKFNHFQSLFINPITRKL
                     HTFNLEKKQIMMFQQIQYLGGHKYVAEKRNAKIIELFNEAPCDYHAVYKLSKFAINQY
                     IKYCRWQNSVLEPTLSAMYQLQLTDQEVVHNYGYIFPEQIYIENHPIEWQLQVDLLLK
                     NGKSKLVNDNLNYFKLKKFIVALESKTAIIEKLINNYLNICSDRGNDVQILF"
     gene            complement(3414..4133)
                     /gene="mecA"
                     /locus_tag="NBH32_002459"
     CDS             complement(3414..4133)
                     /gene="mecA"
                     /locus_tag="NBH32_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HCD1516027.1"
                     /translation="MRIERVDDTTVKLFITYSDIEARGFSREDLWTNRKRGEEFFWSM
                     MDEINEEEDFVVEGPLWIQVHAFEKGVEVTISKSKNEDMMNMSDDDATDQFDEQVQEL
                     LAQTLEGEDQLEELFEQRTKEKDAQGSKRQKSSARKNTRTIIVKFNDLEDVINYAYHS
                     NPITTEFEDLLYMVDGTYYYAVHFDSHVDQEVINDSYSQLLEFAYPTDRTEVYLNDYA
                     KIIMSHNVTAQVRRYFPETTE"
BASE COUNT         1403 a          769 c          559 g         1691 t
ORIGIN      
        1 agacctcgta caccataaat gtaacgaggt ctttttactg actcttaatt ttgcaacatt
       61 aaattattag aacgtaatgt tacacatcga tctaaatata ttagtgatat ttttcgaatt
      121 taaccagttc gaaaattata caaattataa atttggtaca taatagactt atattaatcg
      181 ttaatgtata aaaagtgcat atttaataca tttttataca acttcacttc cctcttatta
      241 aaaaagaaca taaaaaaacg tcgtgtctga atacacgacg ctaaacaaag gggatgggag
      301 aaatttttca cttcaaacaa aggggtatgt ttgttatgtg attaatttca tgttcataat
      361 ataacatgct atctctacca tttcaacctt ttaatagtac ttaatttaaa ttgtcacaat
      421 ctgttcataa tgtaagcgaa ttcaatagta ctaagctttc attaattttt caaaagcgtt
      481 caatttttgt tcaaaaactt cacaagcttg ttcaattggt tcaggtgttg tcatatctac
      541 accagcattt tttaatatct caattggata atttgagcta ccttttttca agaattcatt
      601 aatatatcta tctactgctg gcttaccttc tgttaaaatt tgatgactta agctttgagc
      661 tgcactgtaa ccagttgcgt attgatatac ataataattc atatagaagt gtggaatacg
      721 tgaccattcc ttactaatat cttcatcagt ttctacagaa tcaccgaagt ataatttatt
      781 taatttggca tattcttcat tcattctagt tggcgttaat ggttcacctg cttcttcaat
      841 tgcatgaatt ttatgctcaa attctgcgaa cattgtttgt cggaataatg tagctctgaa
      901 acgttctaat tcttggttta ataataataa gcgtttttca tcatcaagat gtttatccat
      961 ataatcactt aaaagtgctt cgttacaagt tgatgcaact tcagcgacaa aaatagtgta
     1021 gtcactagaa tttgacggtt ggaattttct actgaagtaa ctatgtgctg aatgcccaaa
     1081 ttcatgaact aatgtgtata agtctgaaat agtattagac cagttaagta gaataaatgg
     1141 attagttaaa tgtgcacctg atgaatagcc acctgaacgt ttacctttat tctcatagac
     1201 atcgacccaa cgattgttta agccttcttt aactacattt aaatattctt cacccattgg
     1261 ttctaatgct tttaacatcc actctttagc ttcttcataa ggcatttcaa acttaatatc
     1321 tttaattaat ggtgtatata aatcatacat tttcaagtca tctaaaccta gcaattcttt
     1381 gcgcaattca gtatatctat gtagcaatgg taaatattta tgtacagttt ttactagatt
     1441 gtcatataca ttttctggaa tatgattatt actcaatgct ttttctcttg cagttttgta
     1501 attgtgtgta cgagcattaa atacattttt cttcacttca cctgctagtg tagcgccaag
     1561 cgtattatta tgagcaccat atgctttata tacatttcta aaagcacttt ctcttagttt
     1621 gcgatcatct gattctaaat acttaataaa tgtaccttgt gttaacgggt gtgcatttcc
     1681 atctttatca atcgcatctt caaatactaa atcagcgttg ctaaacatac cgtatacatt
     1741 tgatggcgtt gataacgcgt cctgcgcttc tgttaataac ttttcagttt cagcatctaa
     1801 aatatgagga cgtttttcat taatcaactt caaatcaaat gcgaattttt gtaatttatc
     1861 atatgaattt acaaatgatt gaattttatc ttcatcaatt tgtaaaatct ctggcactaa
     1921 gaaactccat gccgagctaa atttaataat taattgatgt gctcttgact ccataccagt
     1981 atacttgtcg ttcgttgtat cttggtcttg ttttaggtgc gcgtatacat atactttttc
     2041 taatttagta cctaatgtat cttctaattc taacgcattg tataatgtct cagcactatc
     2101 accaatgtgt cctttaaatt gttcttcttt gcctaactca ttttcaactt ctttaaatgc
     2161 agcctcaaaa gcttcatcat ctttgaaaat tgttgttaag tcccatgtat attcaggata
     2221 tttacgttcc tgttcttctc tcgataattg ttgactcata tgtagtaaac ctcctaataa
     2281 aatataattg atataatttt ctcattttag aacaaaattt gcacgtcatt acctctatct
     2341 gaacaaatat ttaaataatt gttaataagt ttttctataa ttgctgtttt actttctaga
     2401 gcaacaataa attttttcag tttaaaataa ttaaggttgt catttactaa tttgcttttt
     2461 ccattcttta acaataaatc aacttgtaat tgccactcaa taggatgatt ttcaatataa
     2521 atttgctctg gaaaaatata accataattg tgcactactt cttgatcagt taactgtaat
     2581 tgatacattg cacttaaagt gggttctaaa acggaatttt gccagcgaca atatttgata
     2641 tattgattaa ttgcgaactt tgataattta taaacagcat gataatcaca aggcgcctca
     2701 ttaaacaact caataatttt ggcattcctt ttttcagcaa catatttgtg cccgcctaaa
     2761 tattgtattt gttgaaacat cattatttgt tttttctcta aattgaacgt atgaagtttt
     2821 cgagtgattg gattaataaa caaactttga aaatgattga acttcacttt attatgacaa
     2881 taatcaacat catttaataa ccaacttact ttatatccca ttttctttaa accttccgtt
     2941 cgttgaagaa tctgtttata aggaattggc gaatactgta gctcaataac atatttatta
     3001 ttaatcaaaa tatccggaat ttgttttatt tcttttaaaa atggctctat ctctacttta
     3061 catccttgtt gctgtatcat ctgtgctaaa atcaatttac ttttataatg tttaatcgtt
     3121 tcattattaa aacatttttg ttccgctaaa tgtttatgtg caaaatgact aattactttg
     3181 agcccacgct ttaaaataac ttgcttgcca cacaccggac aaaaatattg tgtctttctc
     3241 aatgcagtag ttgctaacac acgttccttt tcttcattta aagctactaa catatcttca
     3301 cctcaaaata ttacacgtaa gtttaactac tattactttt tcagcttttg aaatcaataa
     3361 aaaaagagac tccaaagagt cccctttcat agtgcgttgc ttaattaata tcgttattca
     3421 gttgtctctg gaaaataacg tcgaacttga gctgttacgt tatgactcat aattatttta
     3481 gcatagtcat ttaaataaac ttctgttctg tctgttggat aagcaaattc aagcaattga
     3541 ctgtaactat cattaatgac ttcttgatca acatgactat caaaatgtac agcataataa
     3601 taagtaccat caaccatata taacaaatct tcaaactctg tagttattgg attgctatga
     3661 tatgcataat taataacatc ttctaaatcg ttaaatttca caataattgt tcttgtattt
     3721 ttacgtgctg aagacttttg acgtttagaa ccttgagcgt ctttttcttt tgttcgttgc
     3781 tcgaataatt cttctaattg atcttcacct tctaatgttt gagctaacaa ttcttgaact
     3841 tgttcatcaa attgatcagt tgcatcatca tcagacatat tcatcatatc ttcattttta
     3901 gatttagaaa ttgtgacttc gacacctttt tcaaaggcat gtacttgaat ccataatgga
     3961 ccttctacaa caaaatcttc ttcttcgtta atttcatcca tcattgacca aaagaattct
     4021 tcgccacgtt tgcgatttgt ccataaatct tcacgactaa atccacgggc ctcgatatcg
     4081 ctatatgtta taaacaattt tacagttgta tcatctactc gttctattct catatcatct
     4141 cactccttac agtcgatgaa tagtatccac attgtattaa agcatcaaac aaaatacaat
     4201 ttatttgttt gatttctttc tctaaaatat tttggtaact tataataaca tattcgttgt
     4261 agcaaaacta tttaaatgat ttatacagtt aaagcattaa agcactttct taatttaatt
     4321 ttaccttatc atagcaaatg tttcatccaa aacactctaa tatagagcta tattaacaat
     4381 tgcacatttg atttaaaaga tactaagcaa ttattgtaaa at
//
LOCUS       DAHHOJ010000150         4409 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.151, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000150 DAHHOJ010000000
VERSION     DAHHOJ010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4409)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4409)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4409
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.151"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(230..559)
                     /locus_tag="NBH32_002460"
     CDS             complement(230..559)
                     /locus_tag="NBH32_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516028.1"
                     /translation="MINETKYMRQQITNLIQIIDTIKYNTLMTNWNIQTHIYKFNQIV
                     TNELNKFKGFETSYIINEKQVFYYEIITLLNKRPLRQVDYGNKIQYLNFYHTELSNAL
                     FAIKFAH"
     gene            608..1345
                     /gene="sdpB"
                     /locus_tag="NBH32_002461"
     CDS             608..1345
                     /gene="sdpB"
                     /locus_tag="NBH32_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease SdpB"
                     /protein_id="HCD1516029.1"
                     /translation="MENEKKKYTFKDIAWRDLLIIPIIGVIFYALTIAAYIGPILFGY
                     YKIDSNLIFVATITQALAYTIGIGAFYLFHLKVMPSRLRAGFAYIKKYWLRLSITYII
                     ALILIYTYEYMTQFLPKHLQYSETANELELNKMFEVPAFLPVAFLLIVIVGPIVEEIV
                     FRHILIGELGKKFNFIAMSIVSVFLFAFIHVTDAKSPFEFGPYLILSIILVFTYIKSG
                     RNLGSTIALHIANNFVSFVISVISIYA"
     gene            complement(1370..2062)
                     /locus_tag="NBH32_002462"
     CDS             complement(1370..2062)
                     /locus_tag="NBH32_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family transcriptional regulator"
                     /protein_id="HCD1516030.1"
                     /translation="MNKAERQNLIITAIQQNKKMTALELAKYCNVSKRTILRDIDDLE
                     NQGVKIYAHYGKNGGYQIQQAQSKIALNLSETQLSALFLVLNESQSYSTLPYKSEINA
                     IIKQCLSLPQTRLRKLLKRMDFYIKFDDTQHMTLPMLFSDILIYCTERNVMLVDHRVD
                     DNIKAENVIFIGLLCKHGHWHAVIYDIAQDKTAELEIENIIDISYSFGKTIQTRDISI
                     DNYHQFLNPIDS"
     gene            2447..3244
                     /locus_tag="NBH32_002463"
     CDS             2447..3244
                     /locus_tag="NBH32_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HCD1516031.1"
                     /translation="MTDKTLQQIDKLICSWLKQIDNVIPQLIMEMTTETKRHRFDLVT
                     NVDKQIQQQFQQFLATHFPEHQLLAEEKSNKMITNEINHLWIMDPIDGTANLVKQQEE
                     YCIILAYFYEGKPMLSYVYDYPHKKLYKALRGEGAFCNGIKMEEPPSLKLEDAIISFN
                     AQVMNLDTVQDLFDASFSYRLVGACGLDSMRVAKGQFGAHINTNPKPWDIAAQFLFAE
                     LLNLKMTTLDGKAIDHLKGAPFIISNQACHETVLKILNANGGYQKYR"
     gene            3377..4324
                     /locus_tag="NBH32_002464"
     CDS             3377..4324
                     /locus_tag="NBH32_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000830946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HCD1516032.1"
                     /translation="MKRSSKSKMSLPKKIFLWVFGILVILAIVVVVYVAAKIFITGNK
                     IHNPLDRNHSELRDKKVSLNDGDPFTIALFGVDSDADRKKKGGGERSDSIMILSINPK
                     TKKTEIVSIPRDTRAEIVGRGTTEKIAHAYAYGGPNMAVKSLEKLMNVPIDHYATIDM
                     DGLHNMIDSIGGVDVVSNDTFTVDGVRFTKGQQTHVNGDQALKFIRSRKEEGAGGDFG
                     RQQRQQIVLEAMANKIASPSSITHFNSLMNEIQNNVKTDLTLGDLNTIRSNYKDANDT
                     INKHQLSGQGGIQSDGLYYFIPSEQSKAESTKLLKDNLE"
BASE COUNT         1557 a          580 c          777 g         1495 t
ORIGIN      
        1 atattaccgt aagtcatact gtatattccc aatttataca ttatgtactc atctatttaa
       61 atcttagtaa atgtagttat ataagactaa atctaccatc ttaatcatta tgattataac
      121 tcgattaatg catctttaca atttgaagaa actttttaat tgtataattt atgtaataaa
      181 gtgaatttta ataaataact tgtttaaatg acattttatg aaaaataggt taatgagcaa
      241 atttaattgc aaataatgcg ttagatagtt ctgtatgata aaaattaaga tattgaattt
      301 tgttaccata gtcgacttgt cggaggggac gtttattaag tagtgttata atttcataat
      361 agaaaacttg cttttcgttt attatatatg atgtttcaaa gcctttgaac ttattcaatt
      421 cattagtaac tatttggtta aatttataaa tatgcgtttg aatgttccaa tttgtcatta
      481 aagtattata tttaatcgta tcaatgattt gaatcaaatt agtaatttgt tgtctcatat
      541 acttagtttc gtttatcatg tagaagtacc tccaaaaatc aattattaaa caggaaagga
      601 tataacagtg gaaaatgaaa aaaagaaata cacgtttaaa gatatagcat ggagagattt
      661 gcttataatt ccaattatag gtgttatatt ttatgcactc acaattgcag cttacatagg
      721 accaattttg tttggttatt ataaaatcga tagtaatctg atatttgtag cgactatcac
      781 acaagcttta gcatatacga ttgggattgg cgcattttat ttatttcatt taaaagtgat
      841 gccatcaagg cttcgagctg gttttgcgta tatcaaaaaa tattggctta gattgtcaat
      901 aacgtatatt attgctctca tcttaattta tacatatgaa tatatgacac agtttctacc
      961 taaacatttg caatatagtg aaacagccaa tgaacttgag ctaaataaaa tgtttgaagt
     1021 gcctgcattt ttacctgtag cattcttatt aattgtgatt gtaggtccaa tagttgaaga
     1081 aatcgtattt agacatattt taattggaga attaggtaag aagtttaatt ttatagctat
     1141 gagtattgtt tcggtgttct tatttgcatt tattcatgtg actgatgcaa agtcgccatt
     1201 tgagtttggt ccatatctca tactatccat catattagtt ttcacttata taaaatctgg
     1261 aagaaaccta ggttcaacaa ttgcattaca tatagcaaat aattttgttt ctttcgttat
     1321 atcagtaata agtatttatg cgtagataaa accccatcga ctccatagtt taggaatcga
     1381 tggggtttaa aaattgatga tagttatcaa tggatatgtc tctggtttga atcgtcttac
     1441 cgaatgaata cgaaatatct ataatatttt caatttcgag ttcggcagtt ttgtcttgag
     1501 caatatcata aatgactgca tgccaatgtc catgtttaca caaaaggcca ataaatataa
     1561 cgttttcggc tttaatatta tcatcaaccc tatgatctac taacatcaca tttcgttctg
     1621 tacaataaat taaaatgtcg gaaaacaaca ttgggagtgt catatgttgt gtgtcatcaa
     1681 atttaatata aaagtccatg cgcttaagca actttcttaa gcgtgtttgt ggaagactta
     1741 aacattgttt tataattgcg ttgatttcgc ttttatatgg taatgtcgag tacgactgac
     1801 tttcattaag cactaaaaat aaggccgata attgtgtttc agataagttt aatgcaattt
     1861 tagattgtgc ttgttgtatt tggtaaccac catttttccc ataatgcgca taaattttaa
     1921 caccttgatt ttctaaatca tcaatatctc ttaaaattgt gcgtttggat acgttgcaat
     1981 atttagctaa ttctaaagcg gtcatttttt tattttgttg aattgcagta attattaaat
     2041 tttgcctttc agctttattc atgacagcca cctcctaaaa gatgatatat tacacaaagt
     2101 ataacataag agtgacaatg aggacatttt caatatcttt ttaaaaaaat cggaaaaatt
     2161 ttgtagaaaa cgcttacatt ttaaaaaaga ttgtgctatg atattacttg taacaggggg
     2221 agcgattaaa caaaggggta gagctatata acagatagct tttaatcgtt caagttacat
     2281 cacaacatct tcaatattta ttacttactt tcctttctat ttgtcggcta gcacgtgact
     2341 agccgatttt tttatgcaca aaaagaagaa gtcttcagtt gtattgttga ttcttttaat
     2401 atagggtaat agtaaaatga taatgatatg agaagtggtg gacgaagtga cagataaaac
     2461 attacagcaa atagataagt taatatgttc gtggttgaaa caaatagaca atgttattcc
     2521 acaattaatt atggagatga ctacagaaac caagcgtcat cgatttgatt tagttacaaa
     2581 tgtagataaa cagattcaac aacaatttca acaattttta gcaacgcatt ttccggaaca
     2641 ccaactatta gcggaagaaa aaagtaataa aatgattacg aatgaaatta atcatttatg
     2701 gattatggat cccattgatg gaactgccaa tttagtaaaa cagcaagaag agtattgtat
     2761 tatattggcg tatttttatg aaggtaaacc aatgttatcg tatgtatatg attatccgca
     2821 taaaaagctt tataaagcac tacgaggaga aggtgctttt tgtaatggaa ttaagatgga
     2881 agaaccacca tcgttaaaat tggaagacgc tattatatcg tttaatgcac aagtgatgaa
     2941 tctggatacg gtgcaagatt tatttgacgc ctcatttagt tatcgtttag ttggtgcttg
     3001 cggattagat tctatgcgag tggcaaaagg tcaatttggg gcacatatta atacaaatcc
     3061 taaaccttgg gacattgctg cgcagttttt atttgcagaa ctattgaatc taaaaatgac
     3121 aactttagat ggtaaggcaa ttgatcattt aaagggtgca ccttttatta taagtaatca
     3181 agcatgtcat gaaacggtac ttaaaatttt aaatgcaaat ggtggttatc aaaaatacag
     3241 atagatattc tatagtgata aaaaatgata aaaaatagtt atacgtctac agaagtatag
     3301 taaaaaaatt attttactat ataataaata gagaaaacag acaaagtatg taaggaatag
     3361 taaaggagtg gtgggattga aacgttcgtc taaaagtaaa atgagcctac cgaaaaaaat
     3421 atttttatgg gtattcggta ttttagtaat cttagcgatt gtagttgttg tgtatgtagc
     3481 tgctaaaatt tttattactg gtaataagat acacaatccg ttagatcgca atcattcaga
     3541 attaagagat aaaaaagtga gtttaaacga tggtgatcca ttcacaatcg ctttatttgg
     3601 tgtcgattca gatgctgatc gtaagaaaaa gggtggcgga gagcgtagtg atagtattat
     3661 gattttatct atcaacccta aaacgaagaa aactgaaata gtaagtatac cgcgagatac
     3721 aagagcagaa attgttggcc gtggtactac tgaaaaaatt gcacatgcct atgcatatgg
     3781 tggaccaaat atggctgtta aatcacttga aaaattaatg aatgtgccaa ttgatcatta
     3841 tgcgacaatc gatatggatg gtttgcataa tatgatagat agcatcggcg gtgttgatgt
     3901 agtaagtaat gatacattca ctgttgatgg tgttcgtttt acaaaaggac agcagacaca
     3961 tgtaaatggt gatcaagcat tgaagtttat tagaagtcgt aaagaagaag gtgctggtgg
     4021 tgactttggt cgacaacaac gtcagcaaat tgtgcttgaa gcgatggcca ataagattgc
     4081 aagcccatca tcgattacac attttaatag tctaatgaat gaaattcaaa ataatgttaa
     4141 aacagattta acattaggtg atttgaatac gattagaagc aattataaag atgctaatga
     4201 cacgattaat aaacatcaat tgagtggtca aggcggcatt caaagtgacg gattgtacta
     4261 cttcattcct agtgagcagt caaaagctga aagtactaag ttattaaaag ataatctaga
     4321 gtagtaaact aagggaaagt aagttatcaa aattgtacac aaactaatta gatagaatga
     4381 gctgtaaaaa gaataacgaa tagaattaa
//
LOCUS       DAHHOJ010000151         4312 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.152, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000151 DAHHOJ010000000
VERSION     DAHHOJ010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4312)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4312)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4312
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.152"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            50..1330
                     /locus_tag="NBH32_002465"
     CDS             50..1330
                     /locus_tag="NBH32_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HCD1516033.1"
                     /translation="MKKLNIALLGLGTVGSGVVKIIEENRQQIQDTLNKDIVIKHILV
                     RDKSKKRPLNISQYHLTEDVNEILNDDSLDIIVEVMGGIEPTVDWLRTALKNKKHVIT
                     ANKDLLAVHLKLLEDLAEENGVALKFEASVAGGIPIVNAINNGLNANNISKFMGILNG
                     TSNFILSKMTKEQTTFEEALDEAKRLGFAEADPTDDVEGVDAARKVVITSYLSFNQVI
                     KLNDVKRRGISGVTLTDINVADQLGYKIKLIGKGIYENGKVNASVEPTLIDKKHQLAA
                     VEDEYNAIYVIGDAVGDTMFYGKGAGSLATGSAVVSDLLNVALFFESDLHTLPPHFEL
                     KTDKTREMMDSDAEINIKEKSNFFVVVNHVKGSIENFENELKAILPFHRSLRVANYDN
                     QSYAAVIVGLESSPEELITKHGYEVDKVYPVEGV"
     gene            1336..2397
                     /locus_tag="NBH32_002466"
     CDS             1336..2397
                     /locus_tag="NBH32_002466"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCD1516034.1"
                     /translation="MRRWQGLVEEFKAHLPVNENTPKLTLNEGNTPLIHCENMSKILG
                     IDLYVKYEGANPTGSFKDRGMVMAVTKAKEQGKKIVICASTGNTSASAAAYAARAGLK
                     AIVVIPEGKIALGKLSQAVMYGAEIVSIEGNFDEALEIVKEIAKSGEIELVNSVNPFR
                     IEGQKTGSFEIVQQLDGEAPDILAIPVGNAGNITAYWKGFKEYHEAKGSQLPKMFGFQ
                     AEGASPIVQNKVIKNPETIATAIRIGNPASWDKATNALKESNGLIDSVTDDEILEAYQ
                     LMTTKEGVFSEPASNASIAGLIKLHRQGKLPQGKKVVAILTGNGLKDPDTAISLLDNP
                     IKPLPNDKDSIIDYIKGAL"
     gene            2399..3313
                     /locus_tag="NBH32_002467"
     CDS             2399..3313
                     /locus_tag="NBH32_002467"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCD1516035.1"
                     /translation="MSNVLELTIPASTANLGVGFDSIGMALDKFLHLSVKETSGTKWK
                     YIFHDDASKQLPTDETNFIYHVAQQVASKYSVDLPNLCIEMRSDIPLARGLGSSASAL
                     VGAIYIANYFGDIQLSKHEVLQLATEIEGHPDNVAPTIYGGLIAGYYNDVSKETSVAH
                     IDIPDVDVIVTIPTYELKTEASRRALPQKLTHSEAVKSSAISNTMICALAQHNYELAG
                     KLMQQDGFHEPYRQHLIAEFDEVKTIASQHNAYATVISGAGPTILIFSRKENSGELVR
                     ALNRNVVTCHSELVDINVSGVKERIVYQ"
     gene            3371..4174
                     /locus_tag="NBH32_002468"
     CDS             3371..4174
                     /locus_tag="NBH32_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HCD1516036.1"
                     /translation="MTNYKVVVLDMDDTLLNSDNVISEETANYLTAIQDEGYYVVLAS
                     GRPTEGMIPTARDLKLPEHHSYIISYNGSKTINMTNEEVEVSKSIGKQDFDEIVDYCR
                     DRGFFVLTYHDGQIIYDSEHEYMNIEAELTGLPMKRVDDIKAYIQGDVPKVMGVDYVA
                     NITEARIDLNGVFNDNVDATTSKPFFLEFMAKDVSKGNAIKALCHKLGYSVDQVIAFG
                     DSMNDKSMFEVAGLAIAMGNASDELKQYADEVTLDHNENGIPHALKKLL"
BASE COUNT         1570 a          580 c          871 g         1291 t
ORIGIN      
        1 ttcaataata catttgtttt tctcaaaagc acaaacggag gtacataaaa tgaaaaaatt
       61 aaatatagca ttattaggat taggtactgt cggatctggt gttgttaaaa tcatcgaaga
      121 gaaccgacag caaattcaag atacattaaa taaagatatt gtcataaagc atattcttgt
      181 tcgagataaa tctaaaaaga gaccgctaaa tattagccaa tatcatttaa ctgaagatgt
      241 taatgaaatt ttaaatgatg attcattaga tattatcgtt gaagtcatgg gaggaattga
      301 accaactgta gattggttaa gaacagcact taaaaataaa aaacatgtta ttaccgcaaa
      361 taaagattta ttagcagtac atcttaaact tttagaagat ttagcagaag aaaatggtgt
      421 agctttaaag tttgaagcga gtgtagcagg tggtattccg atcgtaaatg ccataaataa
      481 tggtttgaat gcgaataata tttcaaaatt tatgggaatt ttaaatggta cctctaattt
      541 tattttatct aaaatgacta aagagcaaac gacatttgag gaagcacttg atgaagcgaa
      601 aagacttggt tttgctgaag cggatccaac tgatgatgta gaaggggtag atgcagcgcg
      661 taaagttgtc attacatcat atttatcatt taaccaagtc attaaattaa acgacgttaa
      721 acgaagagga attagtggcg taactttaac tgatattaat gtagccgatc aactggggta
      781 taaaattaaa ttgattggta agggaatata tgaaaatggc aaagttaatg catcggtaga
      841 accaacgtta attgataaaa agcatcaatt agcagctgta gaggatgaat ataacgcgat
      901 ttatgtcatt ggtgatgccg ttggtgacac gatgttttat ggaaaaggag caggcagttt
      961 agcaacaggt agtgccgttg tcagtgattt attgaatgta gcattattct ttgaatcaga
     1021 tttacacaca ttgccaccac attttgaatt aaagacagat aaaacaaggg aaatgatgga
     1081 ttcagatgca gaaattaata ttaaagaaaa atccaatttc tttgtagtag tgaatcatgt
     1141 caaaggttca attgaaaatt ttgaaaatga gttaaaggca atattaccat ttcaccgatc
     1201 attaagagtt gcaaattacg ataatcaatc atatgccgct gttatagttg gattggaatc
     1261 atcaccggaa gaattaatca ctaagcatgg atacgaagtt gacaaagtat acccagtaga
     1321 aggagtttaa ttataatgag aagatggcaa ggattagtag aagagtttaa agcacattta
     1381 ccagtaaatg aaaatacacc aaaattaaca ttgaacgagg gaaatacacc actcattcat
     1441 tgtgaaaata tgtctaaaat actaggtata gatttatatg tgaagtatga aggtgccaat
     1501 ccgacaggtt catttaaaga tcgcggtatg gtaatggctg tgacaaaagc aaaagagcaa
     1561 ggtaagaaaa ttgtaatatg cgcttcgact ggaaatacat cagcgtctgc agcagcatat
     1621 gcagcgagag caggtttaaa agctatcgtc gtaataccag aaggtaaaat tgcattaggt
     1681 aaattgtcgc aagcagtaat gtatggtgca gaaatcgttt ctattgaagg aaactttgat
     1741 gaagctttag aaattgtaaa agaaattgca aaaagtggcg aaatcgagct tgtaaactct
     1801 gtcaatccat ttagaatcga aggacaaaag acaggctcat ttgaaattgt acaacaatta
     1861 gacggtgaag cacctgatat tttagcgatt cctgtaggta atgcaggtaa tattactgca
     1921 tattggaaag gctttaaaga atatcatgaa gctaaaggat cacaattgcc gaaaatgttt
     1981 ggcttccaag ctgaaggcgc atcaccaatt gttcaaaata aagtcattaa aaatcctgaa
     2041 acgattgcaa ctgctattcg aattggtaat cctgctagtt gggataaggc gactaatgct
     2101 cttaaagaat caaatggatt aatagatagt gttactgatg atgaaattct agaagcatat
     2161 cagttaatga caactaaaga aggtgtcttt agtgaaccag caagtaatgc ttctattgca
     2221 ggtttaatta aattgcatag acaaggtaaa ttacctcaag gtaaaaaagt agttgctatt
     2281 ttaactggta atggattaaa agatcctgat actgctattt cactactaga taatccgata
     2341 aagccattgc caaatgataa agatagcatt atcgattata ttaaaggagc tttataacat
     2401 gtcgaatgtt ttggagttaa caattcctgc atcaacagcc aaccttggag ttggctttga
     2461 ttctataggt atggctttag ataaattttt gcatctgtct gtaaaggaaa catcagggac
     2521 aaaatggaaa tatattttcc atgatgatgc atcgaagcaa ttgcctactg acgaaacaaa
     2581 ctttatttat catgtagcac aacaagttgc ttctaaatat agtgttgact tgcctaattt
     2641 atgtatcgaa atgagaagtg atattccatt ggcaagaggg ttaggttcgt cagcttctgc
     2701 tttagtagga gctatatata tcgcaaatta ttttggtgac atccaactgt ctaaacatga
     2761 ggtattacaa ttagcgactg aaatcgaagg acatcctgat aatgttgcgc cgaccattta
     2821 tggtggttta atcgctggat attataatga tgtctcgaaa gaaacgtcag ttgcacatat
     2881 cgacatacca gacgtggatg tgattgtaac gataccaact tatgaactta aaacagaagc
     2941 atcaagacgt gctttaccac aaaaattaac acatagtgaa gcggttaaaa gtagtgcaat
     3001 tagtaataca atgatttgtg cattagcaca gcacaattat gaattagcag gtaaactcat
     3061 gcaacaagat ggctttcatg aaccgtatcg tcagcattta attgctgaat ttgatgaagt
     3121 gaaaacaatt gctagtcaac ataatgccta tgcaactgta attagtggtg ctggaccaac
     3181 tattttaata tttagtcgca aagaaaatag tggcgaatta gttcgcgctt taaatagaaa
     3241 tgtagttaca tgtcattctg aattagtaga tattaatgtt agtggtgtta aagaacgaat
     3301 tgtataccaa tagacacttt atattgtaaa atagtattga atgtgaaata gagaggagat
     3361 ttaatgcgat atgacaaatt ataaagttgt cgttttagat atggatgaca cattgctaaa
     3421 ttcagataat gtgatatcag aagaaactgc aaattattta acagcaattc aagatgaagg
     3481 ttattatgtt gttttagcat ctggtagacc tactgaaggt atgattccaa ctgctagaga
     3541 tttaaaatta cctgaacatc atagctatat tattagttat aacggcagta aaacgattaa
     3601 catgactaat gaagaagtag aagtaagtaa atcgattggt aagcaggatt tcgatgaaat
     3661 tgtagattat tgtcgagata gaggattttt cgttcttaca tatcatgatg gtcaaattat
     3721 atacgacagc gaacatgaat atatgaatat tgaagcggaa ttaactggtt taccgatgaa
     3781 acgtgttgat gatatcaaag catatattca aggcgatgta cccaaggtca tgggtgtaga
     3841 ttatgtagcg aatattacag aagctagaat tgatttgaat ggtgtgttca atgataatgt
     3901 agatgctacg acaagtaagc cattcttctt agaatttatg gccaaagatg tttcaaaagg
     3961 taatgcaatt aaagcgttat gtcacaaatt gggatattcg gtggatcaag tcattgcttt
     4021 tggtgatagt atgaatgata aatcaatgtt tgaagtcgca ggtctagcta ttgctatggg
     4081 gaatgcatca gatgaactta agcaatatgc agatgaagtt acgttggatc ataatgaaaa
     4141 tggtattccg catgcgctca aaaaattgtt ataaatttta aaataagcct taacacatga
     4201 tatttgaata agattgaaat gctcctttca aagtagacat tgaaaaaatg aaagctttga
     4261 agggagcttt atttatgtga aaatgacttc agttatacta tagagtattt ga
//
LOCUS       DAHHOJ010000152         4285 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.153, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000152 DAHHOJ010000000
VERSION     DAHHOJ010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4285)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4285)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4285
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.153"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..450
                     /locus_tag="NBH32_002469"
     CDS             <1..450
                     /locus_tag="NBH32_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001209857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516037.1"
                     /translation="HNYIVEKARRNSSFIGYLFKEFDNKNKHYDYAVILATGYYLFGD
                     NFEVHKSRKEIGILGREDECINNIKKIENYNVGKFEDFKNNIMESIRRLYEKDRKEFY
                     ELLSKLNGDVLDLNEIIKYMKTLTVGDVIKVIKSSSFVNFKINFEEV"
     gene            453..743
                     /locus_tag="NBH32_002470"
     CDS             453..743
                     /locus_tag="NBH32_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000514001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516038.1"
                     /translation="MGKEIYLDPIEFQNVLEKFEKGSSEISEVSISSIKDAKNEIILE
                     SIVELEKIIDDLSKAMKTYSKMIGSDKEAMDKIASTMQRNDKDNANKIANRN"
     gene            756..2018
                     /locus_tag="NBH32_002471"
     CDS             756..2018
                     /locus_tag="NBH32_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001137778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516039.1"
                     /translation="MPRLVNSELEEYFNKTNDVKTKLNEVLYNHYTQCYFLSKTGSIK
                     GKTADSIKKYVESAHISTISRAINLMDKLEASVKHLKELGEKCDKSSNAKIGLQTLNE
                     TKKGVVSKKKEFIDLTSNSPAILSEASEFIDTVSLGKDNVESDYNESSKKIDRTKEKL
                     KTMDQTGKQSLESLSNDVRGLITQINKLNNEYHDKNGIIVSKINSITKENWYKNETNK
                     GEFKKMKADHPVAYAEGDETIGRTQAISKDGHIVGTAEIGKVSGIAYRSDDSMGVKGD
                     AKVAGLDGKVKYENVEGKAGLEVGSVKGSAEISNSHISAIAEAQLIDAEAQLRFGSED
                     NNLHVKGDAKVLSASASGSLGADGVEAKAEAVGANTGVEGGFALSGLNFDFGASIGAQ
                     AGGGVNVGKKGIEVDAKFILGGKIKITW"
     gene            2031..2444
                     /locus_tag="NBH32_002472"
     CDS             2031..2444
                     /locus_tag="NBH32_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000047129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5082 family protein"
                     /protein_id="HCD1516040.1"
                     /translation="MSKSSLILEKNYKLQQSAETKTKIAELNDKIERATRIKNKLNDL
                     KKEVKEHRTNSKNIFKEYSYWKGENNSKWEEKSESELISGSITNCIKNIDDNIDRLID
                     KITEMQNEVNSLGSTLSVLENAINSIAAAIEKLTN"
     gene            2455..2928
                     /locus_tag="NBH32_002473"
     CDS             2455..2928
                     /locus_tag="NBH32_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000411307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5083 family protein"
                     /protein_id="HCD1516041.1"
                     /translation="MEKIKRINKIFLSFEVIPFAMMILLAFLPVIAGIINNRLQYGRD
                     FGDRVSFYCYISIMVAYAITILLEIIFYFILLKNYQYDQNNIELANRVKVTKVLSILY
                     MIPGLYAIACYFRFIHVRSFRENKKSGQPAYHFWEFFIKPNRKPIELKDFFIKNR"
     gene            2943..3317
                     /locus_tag="NBH32_002474"
     CDS             2943..3317
                     /locus_tag="NBH32_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000825543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4467 domain-containing protein"
                     /protein_id="HCD1516042.1"
                     /translation="MKRLIIGVLIIMMSIINYGCGNEQNENKYQSQINKVMKIQQETH
                     KEMVKKSNEVNPEFNKDKVNTYVFDDGKLIIISYKLFKDKDQMFYATYEFKNDKIYYK
                     RDINPKTYVKEHQSDYKDIKVK"
     gene            3335..3724
                     /locus_tag="NBH32_002475"
     CDS             3335..3724
                     /locus_tag="NBH32_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020361182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1516043.1"
                     /translation="MEKLIKVTSLIGIIIQSFLTLLFLIFLILSATGIIQPELTTTVN
                     GEQTIQSPETAQATFVTIFAILFVVSLASDLLGIIAMKKLFVNNRASGILYIIGAVIS
                     ANLLTFIAWLISGISVLRYNKIGKEVS"
     gene            3726..4118
                     /locus_tag="NBH32_002476"
     CDS             3726..4118
                     /locus_tag="NBH32_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000878106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5084 family protein"
                     /protein_id="HCD1516044.1"
                     /translation="MKYTWWILLTIAGILSLTSVYGFILCLGSFGMLALNVMWLFVYT
                     PHKNSKALESISKPTIILSIIGTYAVFIFMSILFYFVMKARFMEIGIKLYGEPFNMFG
                     IPLFIIGIILFTIGTVFVYKIQQSRLKQ"
BASE COUNT         1740 a          422 c          799 g         1324 t
ORIGIN      
        1 cataattata tagttgaaaa agcacgtagg aactcttcat ttattggata cttatttaaa
       61 gagtttgata ataaaaataa acattatgat tatgcagtaa ttttggctac tggatattac
      121 ttatttggcg ataattttga agtgcacaag tctagaaaag aaataggtat tttaggtagg
      181 gaagatgaat gtataaataa tataaaaaaa atagaaaatt acaacgttgg aaaatttgaa
      241 gattttaaaa ataatatcat ggaaagtatt aggcgattat atgaaaaaga tagaaaagaa
      301 ttttacgaat tgctatctaa actgaatggt gatgtgttgg atttgaatga aataataaaa
      361 tatatgaaaa ctttaaccgt aggagatgtt ataaaagtta ttaagagttc tagttttgtt
      421 aattttaaaa ttaactttga ggaggtttaa gtatggggaa agaaatttat ttagatccaa
      481 ttgaatttca aaatgtatta gagaaatttg aaaaaggatc aagtgagatt agtgaagttt
      541 caatttctag tataaaagat gctaaaaatg aaattatatt agaaagtata gttgaactag
      601 aaaaaataat tgacgactta tcgaaagcga tgaaaacgta ttctaagatg attggcagcg
      661 ataaagaagc aatggataaa atagcatcaa caatgcaacg gaatgataaa gataatgcaa
      721 ataaaattgc aaataggaat tagggagggg aaaatatgcc tagattagtt aattcagagt
      781 tagaagaata ttttaataaa acaaatgatg tgaaaacgaa attaaatgaa gtattatata
      841 atcattacac tcaatgttat tttttatcta aaactggtag tataaaagga aaaacagcgg
      901 atagtataaa gaaatatgtt gaatcagcac atataagcac aatttctaga gctattaatt
      961 taatggataa gttggaagca tcagtaaagc acttaaaaga acttggagaa aaatgcgata
     1021 aaagtagtaa tgccaaaatt ggtttacaaa ctttgaatga aactaaaaaa ggtgtggtct
     1081 caaaaaagaa ggaattcata gatcttacaa gtaattcccc agcaattcta tctgaagcat
     1141 cagaatttat tgatactgtg tctttaggga aagataatgt agaaagtgat tacaatgaaa
     1201 gttccaaaaa aatagataga actaaagaaa aacttaagac tatggatcaa acaggtaagc
     1261 aatcactaga aagtctaagc aatgatgtaa gaggattaat aactcaaata aataaattga
     1321 ataatgaata tcatgataaa aatggaatta tcgtatcaaa gataaactcc attacaaaag
     1381 aaaattggta taaaaatgaa actaacaagg gtgaatttaa aaaaatgaaa gcggatcatc
     1441 ctgttgctta tgcagaagga gatgagacaa taggtagaac tcaagcaatt tctaaagatg
     1501 gacatatagt tggtacagct gaaataggta aagttagtgg aatagcatat cgaagcgacg
     1561 attcgatggg ggttaaagga gatgctaaag tagctggatt agatggtaag gtaaagtacg
     1621 agaatgttga aggtaaggca ggactcgaag tagggagtgt aaaaggttca gctgaaatta
     1681 gtaactcgca tatatctgct attgcagaag cgcaattaat tgatgcggaa gcacaattaa
     1741 gatttggatc agaagataat aatttgcatg taaaaggaga tgcaaaagtt ttgtcagcaa
     1801 gtgcgagtgg atctttaggt gctgatggag tagaagcgaa agctgaagca gtcggagcta
     1861 atacaggggt tgaaggtggt tttgcactta gtggattaaa ttttgatttt ggcgcgagta
     1921 ttggggctca agctggggga ggtgtaaatg ttggcaaaaa aggaattgaa gttgatgcta
     1981 aatttatact tggaggaaaa attaaaatta catggtaaag gaagaataaa atgtcgaaat
     2041 catctttaat attggaaaag aattataaac ttcaacagtc agcagaaact aaaaccaaaa
     2101 tagcagaatt aaatgataaa atagaaagag caacacgaat taagaataaa ttaaatgatt
     2161 taaaaaagga agtgaaagag catagaacaa attcaaagaa tattttcaaa gaatattcct
     2221 attggaaggg agaaaataat tcgaaatggg aagagaaaag tgagagtgaa ctcataagtg
     2281 gttcaataac aaattgtata aaaaatattg atgataatat cgatagactc atcgataaaa
     2341 ttaccgagat gcaaaatgaa gttaattcat tgggtagtac attatctgta ctagaaaatg
     2401 caataaattc aatagctgct gctattgaaa aattaactaa ttaaaggagt agaaatggaa
     2461 aaaataaaga gaataaacaa aatattttta tcatttgaag ttattccatt tgcaatgatg
     2521 attttactgg cgtttctacc agttattgca ggtataatta ataatcgttt gcagtatggt
     2581 agggattttg gcgatagagt aagcttttat tgttatattt caattatggt tgcatatgca
     2641 attaccattt tgttagaaat tattttttac tttattttat tgaaaaacta tcaatatgat
     2701 caaaataata ttgaattggc taatagagtg aaagttacaa aagtactgtc gattttgtat
     2761 atgattcccg gattatatgc gattgcatgc tattttagat ttatacatgt tcgctcattt
     2821 agagaaaata aaaaatctgg acagcctgct taccactttt gggaattttt tataaaacct
     2881 aatcgaaaac cgatagagtt gaaagacttt tttattaaaa atagataatt aaggagtaac
     2941 caatgaaaag attaattata ggagtgttaa ttataatgat gagtataatc aattatggtt
     3001 gcggtaatga gcaaaatgaa aataaatatc aaagtcaaat taataaagta atgaaaattc
     3061 aacaagaaac tcataaagag atggttaaaa agagtaatga agttaatcct gaatttaata
     3121 aggataaagt aaatacttat gtatttgatg atggcaaatt aattatcatc agctataagt
     3181 tatttaaaga taaagatcaa atgttttacg cgacttacga gtttaaaaac gacaaaattt
     3241 attataagag agatattaat cctaaaacat acgtgaaaga acaccaatca gattataaag
     3301 atattaaagt aaaataattt taaggagtag gaaaatggaa aaattaatca aagtgacgtc
     3361 tttaattgga attattattc aaagtttttt aacattgtta tttttaatat ttttaatttt
     3421 atcagcaaca gggattatac agccagaatt gaccacaact gttaatggtg aacaaactat
     3481 acaaagtcca gaaactgctc aagcaacatt cgtgacaata tttgcaatat tatttgttgt
     3541 ttctttagct tcagatttat taggcattat agctatgaaa aagttgtttg tgaataatag
     3601 agcgagtggc attttgtata ttattggtgc agtaatcagc gccaatttat taacatttat
     3661 tgcttggtta ataagtggga tttctgtttt aagatataac aaaataggaa aagaagtgtc
     3721 atagcatgaa atatacttgg tggatacttt taacaattgc agggatttta agtttaacaa
     3781 gtgtttatgg atttatttta tgtttaggta gctttggaat gttggcactg aatgtcatgt
     3841 ggctatttgt ttatacaccg cataaaaatt ctaaagcact tgaatctata tcaaaaccaa
     3901 cgataatttt atcaatcatt gggacatatg cagtatttat atttatgtct atcttatttt
     3961 acttcgtaat gaaagctagg tttatggaaa ttggaataaa actttacggt gaacctttta
     4021 atatgtttgg aataccatta ttcattattg gcatcatttt atttacaatc ggtacagtat
     4081 tcgtttataa aatacagcaa tcaagattaa agcaatagaa tatattaatt taacatggga
     4141 cgggtttaaa agaaatccgt cctaatgatt tatacataat agatgtagtt gaatctaaat
     4201 gacttcaagc aatttgaaga taaaaagata atgacaatga atgtaaaatg tgattttttc
     4261 aatattaaaa tataaaaatg taatg
//
LOCUS       DAHHOJ010000153         4269 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.154, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000153 DAHHOJ010000000
VERSION     DAHHOJ010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.154"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..995
                     /gene="argF"
                     /locus_tag="NBH32_002477"
     CDS             <1..995
                     /gene="argF"
                     /locus_tag="NBH32_002477"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCD1516045.1"
                     /translation="LRNRSFLTLLDFSRQEVEFLLTLSEDLKRAKYIGTEKPMLKNKN
                     IALLFEKDSTRTRCAFEVAAHDQGANVTYLGPTGSQMGKKETTKDTARVLGGMYDGIE
                     YRGFSQRTVETLAEYSGVPVWNGLTDEDHPTQVLADFLTAKEVLKKDYADINFTYVGD
                     GRNNVANALMQGAAIMGMNFHLVCPKELNPTDELLNRCKNIAAENGGNILITDDIDQG
                     VKGSDVIYTDVWVSMGEPDEVWKERLELLKPYQVNKEMMDKTGNPNVIFEHCLPSFHN
                     ADTKIGQQIFEKYGIREMEVTDEVFESKASVVFQEAENRMHTIKAVMVATLGEF"
     gene            1018..1950
                     /gene="arcC"
                     /locus_tag="NBH32_002478"
     CDS             1018..1950
                     /gene="arcC"
                     /locus_tag="NBH32_002478"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCD1516046.1"
                     /translation="MAKIVVALGGNALGKSPQEQLELVKNTAKSLVGLITKGHEIVIS
                     HGNGPQVGSINLGLNYAAEHNQGPAFPFAECGAMSQAYIGYQLQESLQNELHSIGMDK
                     QVVTLVTQVEVNENDPAFNNPSKPIGLFYNKEEAEQIQKEKGFIFVEDAGRGYRRVVP
                     SPQPISIIELESIKTLIKNDTLVIAAGGGGIPVIREQHDGFKGIDAVIDKDKTSALLG
                     ANIQCDQLIILTAIDYVYINFNTENQQPLETTNVDELKRYIDENQFAKGSMLPKIEAA
                     ISFIENNPKGSVLITSLNELDAALEGKVGTVIKK"
     gene            2121..3677
                     /locus_tag="NBH32_002479"
     CDS             2121..3677
                     /locus_tag="NBH32_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HCD1516047.1"
                     /translation="MENTINESEKKKRFKLKMPGAFMILFILTVVAVIATWVIPAGAY
                     SKLSYEPSSQELKIVNPHNQVKKVPGTQQELDKMGVKIKIEQFKSGAINKPVSIPNTY
                     ERLKQHPAGPEQITSSMVEGTIEAVDIMVFILVLGGLIGVVQASGSFESGLLALTKKT
                     KGHEFMLIVFVSILMIIGGTLCGIEEEAVAFYPILVPIFIALGYDSIVSVGAIFLASS
                     VGSTFSTINPFSVVIASNAAGTTFTDGLYWRIGACIVGAIFVISYLYWYCKKIKKDPK
                     ASYSYEDKDAFEQQWSVLKDDDSAHFTLRKKIILTLFVLPFPIMVWGVMTQGWWFPVM
                     ASAFLIFAIIIMFIAGTGKSGLGEKGTVDAFVNGASSLVGVSLIIGLARGINLVLNEG
                     MISDTILHFSSSLVQHMSGPLFIIVLLFIFFCLGFIVPSSSGLAVLSMPIFAPLADTV
                     GIPRFVIVTTYQFGQYAMLFLAPTGLVMATLQMLNMRYSHWFRFVWPVVAFVLIFGGG
                     VLITQVLIYS"
     gene            3984..4211
                     /locus_tag="NBH32_002480"
     CDS             3984..4211
                     /locus_tag="NBH32_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516048.1"
                     /translation="MTHLTKVLDTLTGICVVLLFSKYFVAYANMVFDWKLRWYLLENI
                     PHLPIILFILMFIFGVPSEMIKDRQRKNNGV"
BASE COUNT         1466 a          590 c          835 g         1378 t
ORIGIN      
        1 atttacgaaa cagaagtttt ttaactttat tagacttttc acgacaagag gtagaattct
       61 tattaacact ctccgaggat ttaaaacgtg ctaaatatat tggcactgaa aagcctatgt
      121 taaaaaataa aaatattgca ctgttatttg aaaaagattc tacaagaaca cgatgtgcat
      181 ttgaagttgc agcacatgat caaggtgcaa atgtaactta tttaggccca actggatcac
      241 aaatgggtaa aaaagaaaca actaaagata ctgcacgtgt gcttggtgga atgtatgatg
      301 gcattgaata ccgtggtttt tcacaaagaa cagtagaaac tttagctgaa tattcaggcg
      361 taccagtgtg gaatggttta actgatgaag atcatcctac tcaagttctt gctgatttct
      421 taacagcaaa agaagtctta aaaaaagatt atgcagatat taactttaca tatgttggag
      481 atggtcgtaa taacgttgca aatgcattaa tgcaaggtgc tgccattatg ggtatgaact
      541 tccatttagt ttgtccaaaa gaattaaatc caacagatga attattaaat cgctgtaaaa
      601 atattgccgc tgaaaatggt ggcaacatat taatcacaga tgatattgac caaggtgtaa
      661 aaggttcgga tgtaatttac actgatgttt gggtatcaat gggtgaacct gatgaagtat
      721 ggaaagaacg acttgaatta ttgaaaccat atcaagtaaa taaagaaatg atggataaaa
      781 ctggtaatcc aaatgttatt tttgagcatt gcttaccatc tttccataat gctgatacga
      841 aaattggtca acaaattttt gaaaaatatg gaattcgaga aatggaagtt acagatgaag
      901 tattcgaaag taaagcttca gttgtattcc aagaagctga gaacagaatg catacaatca
      961 aagcagtcat ggttgctaca ttgggtgaat tttaaatgat ataaggaagt gaatatgatg
     1021 gcgaaaatag tagtagcatt aggtggtaat gctttaggaa aatcacctca agaacaactc
     1081 gagcttgtta aaaatactgc gaaatcatta gtaggattaa taacaaaagg acatgagatt
     1141 gttattagtc atggtaatgg gccacaggtt ggaagcatta atttgggact taactatgct
     1201 gcagaacaca accaaggtcc ggcatttcca tttgctgaat gtggcgcaat gagtcaagct
     1261 tacataggct atcaattaca agaaagctta caaaacgaat tgcattctat tggaatggat
     1321 aaacaagtgg taacactagt gacacaagtt gaagttaatg aaaatgatcc ggcatttaac
     1381 aatccttcaa aaccaattgg gttattttac aacaaagaag aagctgaaca aattcaaaaa
     1441 gaaaaaggat ttatatttgt tgaagatgct ggaagaggat atagacgtgt tgttccttca
     1501 ccacaaccca tctctattat tgaattagag agtattaaaa cacttattaa aaatgataca
     1561 ctcgttattg ctgctggtgg tggagggata ccagtaatta gagagcaaca tgatggtttt
     1621 aaaggtattg atgcagttat agacaaagat aaaacaagtg cgttgttggg tgctaatatt
     1681 caatgcgatc aattgattat tttaacagca attgattatg tatatattaa ttttaacact
     1741 gaaaaccaac agcctttgga aacaacaaat gttgatgaat taaaacgata tatagacgaa
     1801 aatcaatttg caaaaggaag tatgttacca aaaattgaag cagccatatc atttattgaa
     1861 aacaatccaa aaggaagtgt gcttataaca tcattaaatg aattagatgc tgccttagag
     1921 ggtaaagtag gtactgtgat taaaaagtaa ttgaattgaa acgcttttca attactatat
     1981 gtcaaatgca tgatttttat tatttatgtg cacccctgaa aataatgcct ctattttgat
     2041 gcggggtgca ctttcttaat ttatatttat aaaatcttta aggtagaaat gctaggttaa
     2101 atgtcgaagg agatgaaacc gtggaaaata caattaatga aagtgaaaag aaaaaacgat
     2161 ttaaattaaa aatgccaggt gcatttatga ttttattcat tttaacggtt gttgcagtta
     2221 tagcaacatg ggttattcct gctggtgcat attctaaact ttcttacgaa ccttcatccc
     2281 aagaactaaa gatagttaac cctcataacc aagtgaaaaa ggttccgggt acgcaacagg
     2341 aactagacaa aatgggggtt aaaattaaga ttgaacaatt taaatcaggt gcaattaata
     2401 agccggtatc aattccgaat acttatgaaa gattaaagca acatccagct ggaccagaac
     2461 aaataacaag tagcatggtt gaaggtacga tagaagcggt cgatatcatg gtattcattc
     2521 ttgtactagg gggacttatt ggcgtagttc aagccagtgg ttcttttgaa tcgggattgt
     2581 tagctttaac gaagaaaaca aaagggcatg aatttatgct aattgtgttt gtatcaatac
     2641 taatgattat cggcgggaca ttatgtggta ttgaagaaga agctgtagca ttctatccga
     2701 ttttagtccc tatatttata gcgttaggat acgattctat cgtttcagtt ggtgccatat
     2761 tccttgccag ctctgtcggt agtacatttt caactattaa cccgttctcg gttgtaattg
     2821 cctctaatgc cgctggtaca acttttacgg atggcttgta ttggagaata ggcgcttgta
     2881 ttgtcggtgc gatttttgtt attagttatt tatattggta ctgtaaaaaa attaaaaaag
     2941 atcctaaagc gtcatattct tatgaagaca aagatgcttt tgaacagcaa tggtctgtat
     3001 taaaagatga cgatagtgcc cattttactt tgcgtaagaa gataatcctt acattatttg
     3061 tactaccatt tccaattatg gtatggggag ttatgacgca aggttggtgg ttcccagtta
     3121 tggcttcagc atttttaata tttgcaatta taataatgtt tattgctggg acaggtaaat
     3181 ctggattggg agaaaaagga actgtagatg catttgtcaa tggcgcatca agtttagtag
     3241 gtgtatcttt aattattggc ttagctcgag gtattaattt agtgttgaat gaaggtatga
     3301 tttcagatac aatcttacac ttttcatcat ctttagttca acatatgagt ggaccattat
     3361 ttatcatcgt attactattt attttcttct gtttaggttt tatcgtgcca tcatcttctg
     3421 gattagcggt attatcaatg cctatctttg caccactagc tgatacagta ggtataccaa
     3481 gattcgtcat cgttacgaca tatcaatttg gtcaatatgc gatgttattc ttagcgccga
     3541 ctggacttgt tatggccaca ctacaaatgt taaacatgcg atattcacat tggttccgat
     3601 ttgtatggcc ggtagttgct tttgtattga ttttcggtgg cggagtacta attacgcaag
     3661 tactaattta ttcataattt gaaatgctat attataaaaa tactaattgg gttttatgca
     3721 tctcgtaggt ttgtagaaat actaaactaa gcgaggtgca ttattatttt tgattaagaa
     3781 aataatgacg gtaatcataa cactagtaag taattgatac aatgctctat ttaataatga
     3841 tatttttaaa atttgttttt aatgtaatgt tagatctatg gtatattata tttatcgtgg
     3901 taaatatgta tttgctgtaa acagtttttg taacacatga cacatatggt tgtgaaaata
     3961 gcatatataa aggatggcta taaatgacac atttgacaaa ggttttagat acactaactg
     4021 gaatatgcgt agtattatta tttagtaaat attttgtggc gtatgcaaat atggtgtttg
     4081 attggaagtt aagatggtat ttgctagaaa acataccaca tttgccaatt atattattta
     4141 ttctgatgtt tattttcgga gtaccttctg aaatgataaa agataggcaa aggaaaaata
     4201 acggcgttta atttatcgat atttagaggt gataaaattt gtcaactatt acaaagacta
     4261 aaataaaaa
//
LOCUS       DAHHOJ010000154         4219 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.156, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000154 DAHHOJ010000000
VERSION     DAHHOJ010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4219
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.156"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..498
                     /locus_tag="NBH32_002481"
     CDS             <1..498
                     /locus_tag="NBH32_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1516049.1"
                     /translation="NLLLKTYAFIGLLSHDGVINQFFHLFNLPSFNLLFTTGAFLVVA
                     SYIYIPFMILPIFNSMKAIPNNLLQASSDLGASPFYTFRKVIMPLTKEGVMTGIQVTF
                     IPSLSLFMITRLIAGNKVINIGTAIEEQFLTIQNYGMGSTIAIFLIVFMAFILIITKS
                     SNGRG"
     gene            504..1313
                     /locus_tag="NBH32_002482"
     CDS             504..1313
                     /locus_tag="NBH32_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1516050.1"
                     /translation="MKWYGKLYIGILLAILYIPIFFLMFYSFNSAGNMIHFEHFTLEH
                     YQSLFQNDHLMSVIFNTIAVALLAASISTVIGTFGAIAIYYLRNKKFKVTLLTLNNVL
                     MVSSDVVIGASFLIMFTTIGHFTGLGLGFWTVLISHIAFCIPIVVIIVLPQLYEMNNN
                     MLNAARDLGATEPQLLSNIIIPNILPSIIGGFFMALTYSLDDFTVSFFVTGNGFSVLS
                     VEVYAMARKGISMEINAISTLLFAVIVLGILGYYLIQYVINKKKLIKRGVK"
     gene            1313..2386
                     /locus_tag="NBH32_002483"
     CDS             1313..2386
                     /locus_tag="NBH32_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein"
                     /protein_id="HCD1516051.1"
                     /translation="MKRFLQLIIGSLVVGMLCLTLSHWFKSKEQVHTNQKIYVYNWGE
                     YIDPELIKKFEKETGIQVVYETFDSNEAMEAKIRNGGTHYDVAFPSEYTVQKLKRDHL
                     LLPIDHNKVPNIKNLDSDYMNMSFDKGNKYSLPYFFGTVGILYNKEKYPNESFDSWKS
                     LYNPKFKNQILLVDGAREIIGMSLNKLGYNLNDRNSHHLKEAERDLTKLAPQVRGVVG
                     DEITMMLQQNEGNIAVVWSGVAAPLVQEGDKYNYVIPKEGSNLWFDNMVIPKTAQNKE
                     GAYKFMNFLLDAKNNKQNTEFVGYATPNKAARQLLPKEIKDDHRFYPTKKEQERLEVY
                     KDLGPEVLSEYNENFLNFKMSLK"
     gene            2460..3479
                     /locus_tag="NBH32_002484"
     CDS             2460..3479
                     /locus_tag="NBH32_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1516052.1"
                     /translation="MTGEQFTQIKRPVSRLTEKVLGWLCWVMLLVLTVITMFIALVSF
                     SNNTSIANLENTLNNNAFIQQLLAGNGYNTTQFVIWLQNGIWAIIVYFIVCLLISFLA
                     LISMNIRILSGFLFLISAIVTIPLVLLIVTLIIPILFFIIAMMLFIRKDKVEMVAPQY
                     YEEYNGPIYDYREPVYERPQPKDDYYDVPKYEKELDKSNTVYDQEQERDKYDQFPKRA
                     VESEYNHDEHTEEEPSVLSRQAKYKQKSTEELGIEDDGYYAEPEVDPKELKAQQKREK
                     AEIKAKKKEKRKAYNQRMKERRKNQPSAVSQRRMNFEERRQIYNNDISEERNSSEVKD
                     KKEQE"
     gene            3763..4182
                     /locus_tag="NBH32_002485"
     CDS             3763..4182
                     /locus_tag="NBH32_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HCD1516053.1"
                     /translation="MNRKPELIMAWIANSISIIYLLVMGLSYFSLKSGNASQREELAK
                     QLSQNGGNVSLDMLQTTIGALAIILLISTLYGIFATICIKGRRKLSIILFVIAIIVSL
                     MALNLIAIVLWIIVMIMLISKKESKETTHKDDEYIYH"
BASE COUNT         1513 a          542 c          721 g         1443 t
ORIGIN      
        1 aatttattgt taaagacata tgcttttata ggtttattaa gtcatgatgg cgtgattaat
       61 caatttttcc acttatttaa tttaccatca ttcaatttgt tatttacaac tggtgcgttt
      121 ttagtagtgg caagttacat ttatatacca tttatgattt tacctatatt taatagcatg
      181 aaagcaattc ctaataattt attgcaggcc tcaagtgatt taggtgctag tcctttttat
      241 acttttagaa aagtaatcat gccgttaaca aaagaaggtg ttatgactgg gattcaagta
      301 acctttattc catcactttc attatttatg attacaagat taattgcagg taataaagtc
      361 ataaatatag gtacggcaat agaggaacaa tttttaacaa ttcaaaatta tggtatggga
      421 tcaactatag ctatattctt aattgtattt atggcattca ttttaatcat tacaaaatca
      481 tctaatggga gagggtgaat catatgaaat ggtatggaaa gctgtatatc gggatacttt
      541 tagcgatttt atacatccca atattctttt taatgttcta ttcattcaat tcggctggta
      601 atatgattca ctttgaacat tttacattag agcattatca atcattattt caaaatgatc
      661 atttaatgtc ggtcattttt aatacgatag ctgtagcact tttagcagcc tcaatttcta
      721 cagttattgg tacatttggt gccattgcta tttattattt aagaaataaa aagtttaaag
      781 taactttact aacattgaat aatgtcttga tggtatcatc cgacgttgtc ataggtgcat
      841 cattcttaat tatgtttaca acgattggcc attttactgg tcttggttta ggattttgga
      901 cagttctaat atctcatatt gcattttgca tacctatagt tgtgattatc gtcttaccac
      961 aactgtatga aatgaataat aatatgttaa atgctgcaag agatttagga gcgactgaac
     1021 cacaattatt aagcaacatt attattccta atattttacc ttctattata ggaggtttct
     1081 ttatggcttt aacttattca ctagacgatt tcacagtaag tttcttcgtt actggtaatg
     1141 gctttagtgt gttatcagtt gaagtttatg ctatggcgag aaaaggaatt agtatggaaa
     1201 ttaatgcgat ttcaacatta ttgtttgctg ttattgtatt aggaatacta ggatattatt
     1261 tgattcaata cgtgattaat aagaaaaaac taatcaagcg aggtgtaaaa taatgaaacg
     1321 ttttttacaa ctcattatag gttcattagt tgtgggtatg ctttgtctta ctttaagtca
     1381 ttggtttaaa tctaaagaac aagtgcatac aaatcaaaaa atttacgtat acaattgggg
     1441 cgaatatatt gatccagagt taattaagaa atttgaaaaa gaaactggca ttcaagtcgt
     1501 ttatgaaact ttcgattcaa atgaagcgat ggaagccaaa attcgtaatg gcggtacaca
     1561 ttatgatgtt gcttttccta gtgaatatac agttcaaaaa ttgaaaagag atcatttatt
     1621 gttaccaata gatcataata aggtacctaa tattaaaaat ttagattcag attatatgaa
     1681 tatgtcattt gataaaggca ataaatattc tttaccttat ttctttggaa ctgtaggtat
     1741 tttatataat aaagaaaagt atccaaatga atcatttgat agttggaagt cattgtataa
     1801 tcctaaattt aaaaaccaaa ttttactagt tgacggtgct agagagatta taggcatgag
     1861 tttgaataaa cttgggtata atcttaatga ccgtaattcg caccatttaa aagaagcaga
     1921 gcgagattta accaaactag caccacaagt aagaggtgtc gtaggtgatg aaattaccat
     1981 gatgcttcaa caaaatgaag gtaacatagc ggttgtttgg agtggtgttg cagcacctct
     2041 agtgcaagaa ggggataaat ataattatgt tattcctaaa gaaggatcga atttatggtt
     2101 cgacaatatg gtaattccaa aaacggcaca aaataaagag ggtgcatata aatttatgaa
     2161 ttttttatta gatgctaaaa ataacaagca aaatacagaa ttcgtaggct atgcaacgcc
     2221 aaacaaggct gctcgacaat tgttgcctaa agagattaaa gacgaccatc gtttctatcc
     2281 gactaagaaa gagcaagaac gccttgaagt ttataaagat ttaggacctg aagttttaag
     2341 tgaatacaat gaaaactttt tgaatttcaa aatgtcatta aaataaaatc ataaatagta
     2401 gttatttact gtatatttag ttataataac gggataagta ctataaggag ttgtaacgaa
     2461 tgactggaga acaatttact caaattaaac gtccagtaag tagattaact gaaaaagttc
     2521 taggttggtt atgttgggtg atgttattag tgcttactgt cattacgatg tttatagcac
     2581 ttgtttcatt cagtaataac acatcgattg ctaatcttga aaatacatta aacaataatg
     2641 catttatcca gcaattatta gctggaaatg gctataatac aacacaattt gtaatatggt
     2701 tacaaaatgg tatatgggct attatcgttt actttattgt ttgtttgttg atttcatttt
     2761 tagctctcat atctatgaat ataagaatct tgtcaggttt cttattctta atatcagcaa
     2821 ttgtaacgat tcctttagtt ttacttatcg ttactttaat cattccgata ttattcttta
     2881 ttattgcgat gatgctattt ataagaaaag ataaagttga aatggttgcg ccacaatatt
     2941 atgaagagta taacggaccg atttatgatt atcgtgaacc tgtgtatgag cgcccacaac
     3001 cgaaagatga ttattatgat gtgcctaaat atgaaaaaga attggataaa tcaaatactg
     3061 tatatgatca agaacaggaa agagataaat atgatcaatt tcctaaacgt gcagttgaaa
     3121 gtgaatataa tcatgatgag cacactgagg aagaaccatc agtattatcc agacaggcta
     3181 aatacaaaca aaaaagtact gaagaactag gtattgaaga tgatggttat tatgcagaac
     3241 ctgaagttga tccaaaagaa ttgaaagcac aacaaaagcg agaaaaagct gaaattaaag
     3301 ctaagaaaaa agaaaaacgt aaagcttata accaacgaat gaaagaacgt aggaaaaatc
     3361 aacctagcgc agttagtcaa cgtcgaatga attttgaaga gcgacgtcaa atttacaaca
     3421 atgatatttc tgaagaacgc aattcaagtg aagttaagga caaaaaagag caagaataaa
     3481 tattgatgat acccagattt agtaagtaga ggtcttttgc ttattaatct gggtttttat
     3541 atgaggttaa ttatcgataa tgtctaatta aagtgtttag gtgtcaaaat tttaaatgac
     3601 gatttctcat taccatacac ctaaattacc atcaatctga attcagatat ttattataaa
     3661 aattagatga aaaatatgtt aatatacaag taaattaatg tgcgtatatc tataggctcg
     3721 tagtatactt attaaaatat taaacaaaga aaggatttta agatgaatag aaaacctgaa
     3781 ttaatcatgg cttggattgc aaatagtatt agtattattt atttattagt tatggggcta
     3841 tcctattttt ctttaaaaag tggtaatgca tcacaacgcg aagaattagc gaagcaatta
     3901 tctcagaacg gtggcaatgt ttctttagat atgcttcaga caacaattgg tgcattagca
     3961 attattttat taatttcaac actttatggt atatttgcga caatttgtat taaagggcgt
     4021 agaaaattat cgattatact ttttgttatc gcgataattg taagtttgat ggctcttaat
     4081 ttaattgcaa ttgtcttatg gattatcgtg atgattatgt tgatttctaa aaaagaatca
     4141 aaagaaacta cacataagga cgatgagtat atttatcatt aatatgttca tagcaaaaga
     4201 aaaaccatta aaatgttaa
//
LOCUS       DAHHOJ010000155         4037 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.157, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000155 DAHHOJ010000000
VERSION     DAHHOJ010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4037)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4037)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4037
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.157"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            136..507
                     /locus_tag="NBH32_002486"
     CDS             136..507
                     /locus_tag="NBH32_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HCD1516054.1"
                     /translation="MLKKWLGMALITPILTFIIWVFNSHTIITYLNILFYVSLIIFIS
                     IFLILLVQEGIFDATSYGFRRLKYQMSSSKKKKSISDDPFFNPQEVKKEHYFVSTWII
                     PLLLINILYFIMTIVLSLILV"
     gene            complement(562..1806)
                     /gene="fabF"
                     /locus_tag="NBH32_002487"
     CDS             complement(562..1806)
                     /gene="fabF"
                     /locus_tag="NBH32_002487"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCD1516055.1"
                     /translation="MSQNKRVVITGMGALSPIGNDVKTTWENALKGVNGIDKITRIDT
                     EPYSVHLAGELKNFNIEDHIDKKEARRMDRFTQYAIVAAREAVKDAQLDINENTADRI
                     GVWIGSGIGGMETFEIAHKQLMDKGPRRVSPFFVPMLIPDMATGQVSIDLCAKGPNGA
                     TVTACATGTNSIGEAFKIVQRGDADAMITGGTEAPITHMAIAGFSASRALSTNDDIET
                     ACRPFQEGRDGFVMGEGAGILVIESLESAQARGANIYAEIVGYGTTGDAYHITAPAPE
                     GEGGSRAMQAAMDDAGIEPKDVQYLNAHGTSTPVGDLNEVKAIKNTFGEAAKHLKVSS
                     TKSMTGHLLGATGGIEAIFSALSIKDSKVAPTIHAVTPDPECDLDIVPNEAQDLDITY
                     AMSNSLGFGGHNAVLVFKKFEA"
     gene            complement(1818..2759)
                     /locus_tag="NBH32_002488"
     CDS             complement(1818..2759)
                     /locus_tag="NBH32_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HCD1516056.1"
                     /translation="MNVGIKGFGAYAPEKIIDNAYFEQFLDTSDEWISKMTGIKERHW
                     ADDDQDTSDLAYEASVKAIADAGIQPEDIDMIIVATATGDMPFPTVANMLQERLGTGK
                     VASMDQLAACSGFMYSMITAKQYVQSGDYHNILVVGADKLSKITDLTDRSTAVLFGDG
                     AGAVIIGEVSEGRGIISYEMGSDGTGGKHLYLDKDTGKLKMNGREVFKFAVRIMGDAS
                     TRVVEKANLTSDDIDLFIPHQANIRIMESARERLGISKDKMSVSVNKYGNTSAASIPL
                     SIDQELKNGKLKDDDTIVLVGFGGGLTWGAMTIKWGK"
     gene            3054..3239
                     /locus_tag="NBH32_002489"
     CDS             3054..3239
                     /locus_tag="NBH32_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HCD1516057.1"
                     /translation="MKHLVTFFWALLLMQMVNFVLNSLVGGDSLNLVNPIIMAVLFTI
                     FTAIFAAVIKPPKDSSQ"
     gene            complement(3496..3930)
                     /locus_tag="NBH32_002490"
     CDS             complement(3496..3930)
                     /locus_tag="NBH32_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000769690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAP domain-containing protein"
                     /protein_id="HCD1516058.1"
                     /translation="MKLKSFITVTLALGMIATTGATVASNEVSAAEKDKQPATQKAKE
                     MQNVPYTIAVDGIMAFNQSYLNLPKDSQLSYLDLGNKVKALLYDERGVTPEKIRNAKS
                     AVYTITWKDSSKKEVDLKKDSYTANLFDSNSIKQIDINVKTK"
BASE COUNT         1253 a          744 c          590 g         1450 t
ORIGIN      
        1 ttttttgctt taaactaatg aagaatgatt gattttagaa tttcaataaa gctgaagaaa
       61 ttaaaaaatc attcctcaat gtctcatttt aatgtaaact atgcgtaaga gttttctata
      121 agtgggagtg atcaaatgtt gaaaaaatgg cttggaatgg cactaataac gccaatatta
      181 acgtttatta tatgggtttt taatagccat actatcatta catatttaaa tatattgttt
      241 tatgtttcat taattatttt tattagcata tttttgattt tacttgtaca agaaggcatt
      301 tttgatgcga caagttatgg ttttagaaga ttaaagtatc aaatgtcatc ttcgaaaaag
      361 aagaagtcta tctctgatga tccatttttc aacccacaag aggtaaaaaa agagcattac
      421 tttgtttcta catggataat ccccttatta ttaataaata ttctatattt tattatgacg
      481 attgtgctgt cgctcatctt agtataaaga aacagaaaaa atcttcagta actaaaacaa
      541 cgttactgaa gatttttata attatgcttc aaatttcttg aatactaata ctgcgttatg
      601 tccaccgaat cctaagctat tactcattgc ataagtaata tcaaggtctt gcgcttcatt
      661 tggaacaata tccaaatcac attctgggtc tggtgttacc gcatgaattg tcggtgcgac
      721 tttagagtct ttaattgaaa gcgctgagaa gattgcttca attccacctg ttgcaccaag
      781 taagtgacca gtcattgatt ttgttgagct aacttttaag tgtttagctg cttcaccaaa
      841 tgtattttta atagctttaa cttcatttaa gtcaccaaca ggagtacttg taccatgggc
      901 atttaagtat tgtacatctt taggttcaat accagcatca tccatagctg cttgcattgc
      961 tctagaaccg ccttcacctt ctggagctgg cgctgtaata tgataagcat cacctgtagt
     1021 accatagcca actatctcag cataaatatt ggcacctcga gcttgtgctg attctaaaga
     1081 ttcgattact aaaataccag caccttcacc cataacaaaa ccgtctctac cttcttggaa
     1141 tggacgacat gctgtttcaa tgtcatcatt tgtagaaagc gctcgacttg cactgaaacc
     1201 agcaattgcc atatgagtaa ttggtgcttc tgtaccacca gtaatcattg catctgcatc
     1261 accgcgttgc acaattttaa atgcttctcc gattgaattt gtacctgttg cacatgctgt
     1321 aactgttgca ccatttggtc cttttgcaca taagtcaatt gatacttgcc cagttgccat
     1381 atcaggaatt aacattggta cgaaaaatgg actcacacgt cttgggcctt tatccattaa
     1441 ttgtttatgt gcaatttcaa atgtttccat accaccgata ccagaaccaa tccatacacc
     1501 gattcgatct gcagtatttt cattgatatc taattgcgca tctttaacag cctctctagc
     1561 tgctacaatt gcatattgag taaatctatc catacgacgc gcttcttttt tgtcgatatg
     1621 atcttcaata ttaaagtttt taagttctcc tgctaagtga acgctataag gttcagtatc
     1681 gatacgtgta attttatcga taccatttac gccttttaga gcattctccc atgttgtttt
     1741 gacatcatta ccgattggag aaagggctcc catacctgta ataactactc ttttattttg
     1801 actcattcgt tatcctccta ttttccccat tttattgtca ttgcgcccca agttaggccg
     1861 ccaccgaatc cgacaagaac aattgtatca tcatctttga gtttaccatt ttttaattct
     1921 tgatcgatac ttaaaggtat tgacgcagct gaagtatttc catatttatt tacagaaaca
     1981 ctcattttgt cttttgaaat acctaagcgt tctctagctg attccataat tctaatatta
     2041 gcttgatgag gaataaataa atctatatca tctgatgtta aattcgcttt ttcaactaca
     2101 cgtgttgatg catcacccat aattctaaca gcaaatttaa atacttctcg accattcatt
     2161 ttcagtttac cagtatcttt atctaaatat aaatgtttac caccagtgcc atcagaaccc
     2221 atttcataac ttataatacc tctgccttct gaaacttcac cgataataac cgcacctgca
     2281 ccatctccaa atagaactgc agtagaacgg tcagttaaat ctgttatttt agataattta
     2341 tctgcaccga caactaaaat attatgataa tctccagatt gaacatattg tttagctgta
     2401 atcattgaat acataaatcc tgaacatgct gcaagttgat ccatagaggc aactttgccc
     2461 gtccctaaac gttcttgcaa catatttgcg acagttggaa atggcatatc tccagttgct
     2521 gtggcaacaa ttatcatatc tatatcttcg ggctgaatac cagcgtcagc gattgctttt
     2581 acacttgctt catatgctaa atctgaagta tcttgatcgt catctgccca atgtctttct
     2641 ttaattccag tcatcttaga aatccattca tcagatgtat ctaaaaattg ctcaaaatag
     2701 gcattgtcaa taatcttttc tggtgcatat gcaccaaaac ctttaatacc cacgttcata
     2761 gttgaacacc ttcttaataa aatttttaat accaggtatt aatttaacat agattacttt
     2821 ttaaactcaa gtcagaggta ggaatattga ctagttaata aaattttaat gctttattat
     2881 cgtcaaattc cagtatgaaa tttgttttaa tgtgcttaaa ttagaatagc cattcagata
     2941 aaatcattta tttaaactgt ataatatggt caaaatatat gagatgttac tatacaaatt
     3001 tctaactttc acttaaaata aaatatatat aattaaagtg gaggtagaat gacatgaaac
     3061 acctcgtaac attcttttgg gcattattat taatgcaaat ggttaacttt gtacttaata
     3121 gcctagtagg tggagacagc ttaaatttag tgaatccaat tatcatggca gtattgttca
     3181 cgattttcac agcaattttt gctgcagtaa ttaaaccgcc gaaagattca tcacaataac
     3241 tatttagtat aaaatgaaat atgtctttct tattattgaa gctgcccaga taaaggcggc
     3301 ttttattttt ggccattaat gttttgtatt ttgaatattt taaagctcaa tgacctctaa
     3361 cccatcactt ttaaatttag aaaaagcctt aatacaaaga tgtcatttac gagtaatctt
     3421 tgtatcaagg caattatatt ataatcatca ctgtaaaatt aaatgcttca tgttgaattt
     3481 gataatttta agtgtttatt tagtttttac attaatatca atttgtttaa ttgaatttga
     3541 atcaaacaag tttgctgtgt agctatcttt cttaagatcc acttcttttt tactactatc
     3601 tttccaagta atcgtgtaaa cggcagattt tgcatttcga atcttctcag gtgttacacc
     3661 gcgttcatca tataataaag ctttaacttt atttcctaaa tctaaatatg ataattggct
     3721 atcttttggt aaatttaagt aagattgatt gaaagccata atgccatcta ctgcaattgt
     3781 atatggaaca ttttgcattt ctttagcttt ttgagttgct ggttgtttgt ccttttctgc
     3841 tgcagatacc tcattacttg ccacagtagc gccagtcgtt gcgatcatgc ccagtgccaa
     3901 agttacagtt ataaatgatt ttaatttcat ataatctctc tcctttgtga gttaaagtat
     3961 cttacaaaat caatataaca caaattgaaa agtttcaaaa attataagta tgtaaattgt
     4021 ttacttgttt ttaaaaa
//
LOCUS       DAHHOJ010000156         3980 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.158, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000156 DAHHOJ010000000
VERSION     DAHHOJ010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3980)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.158"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..1009
                     /gene="secF"
                     /locus_tag="NBH32_002491"
     CDS             <1..1009
                     /gene="secF"
                     /locus_tag="NBH32_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCD1516059.1"
                     /translation="FGVKKNKRHDINEGVDVHDLKTSFEKWNFVKLAKPLIGVSILIV
                     VVGLVILYIFKLNLGIDFSSGTRVDFQSKQAITQQKVEQVVKDSGLKADQIQINGKDN
                     KVATVQFKDDLTRAQDNKLSDNIKSKFGDTPQINTVSPIIGQELAKNAMLALIYASIG
                     IIIYVSLRFEWRMGLSSVLALLHDVFIIVAIFSLFRIEVDLTFIAAVLTIVGYSINDT
                     IVTFDRVRENLQKVKVITTTEQIDDIVNRSIRQTMTRSINTVLTVIVVVVAILFFGAP
                     TIFNFTLALFIGLISGVFSSIFIAVPLWGIMKKRQLKKSPKHKLVVYKEKKSNDEKIL
                     V"
     gene            1212..3485
                     /gene="recJ"
                     /locus_tag="NBH32_002492"
     CDS             1212..3485
                     /gene="recJ"
                     /locus_tag="NBH32_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCD1516060.1"
                     /translation="MIKPKYKWKLTKPAEYISDELTSKLKLTPIVKKILESKSIIDEQ
                     TIEAIISDTDINHDALQLSDMTKTIERIKRAIANDEKILVYGDYDADGVTSTTILVTT
                     LQLLGAQVGWHIPNRFTEGYGPNELAFRNAHDEGITLIITVDNGIQGHNEIKMVQDLG
                     VDVIVTDHHEIGSTLPEAYAIVHPMHPSFNYPFQQLCGAGVAYKLAQALIENVPDYFK
                     ALVAIGTIADLVSLTDENRSLVKQGLKVLNDQCPTSVKALLKEAGYNDNIDEETIGFI
                     IGPRLNAVGRLDDASLACELLMTDDEEEAAFLAEQVEHFNRERKDIVATITEEAMAMA
                     ETKVKKGDLFLLLAKENWHEGVLGIVASKIVETFALPTLILNIDREQNHAKGSARSID
                     QVSMFEILSAHQELIAKFGGHHMAAGMTMDIENIESLAEGLNKWMKELSKTTSLDPVK
                     PVDVLLTENDITIKNIRDMNRLRPFGTDFSRPIFEMDDLSVSSVKAIGQQKNHLKLTL
                     GESNIAALFWQNGHLEPELQDEQPINILGSVQINEWNGNQSPQLIIQDIAMNEQQILD
                     YRSKRKSLPFTENDENIVVLIHPKSDKVNANEYYYGEEIKHQTDKVVLRDLPTSMEDL
                     SNSLQQLQFSQLYIVLQHNHSIYFDGIPNMDIFKKCYKALITKQETNIQKEGMLLCQH
                     LSVKPDTLKFMLKVFLDLKFVTQEDGLIRINQQPDKRSIDSSKVYQLRQQRMDVEKQL
                     LYQDFSEIKNWIKSQLS"
     gene            3507..>3980
                     /locus_tag="NBH32_002493"
     CDS             3507..>3980
                     /locus_tag="NBH32_002493"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009559512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCD1516061.1"
                     /translation="MDLKQYVSEVQDWPKPGVSFKDITTIMDNGEAYGYATDKIVEYA
                     KDRDVDIVVGPEARGFIIGCPVAYSMGIGFAPVRKEGKLPREVIRYEYDLEYGTNVLT
                     MHKDAIKPGQRVLITDDLLATGGTIEAAIKLVEKLGGIVVGIAFIIELKYLNGIEK"
BASE COUNT         1470 a          541 c          734 g         1235 t
ORIGIN      
        1 atttggtgtt aaaaagaata aacgacatga tattaatgaa ggtgtagatg ttcatgacct
       61 taaaacttca ttcgagaaat ggaattttgt taaattagct aagccattaa ttggagtaag
      121 tattttaatt gtagttgtcg gtttagttat tctttatatc ttcaagttaa acttaggaat
      181 tgatttctca agtggtacac gtgtagattt ccaatctaaa caagcaatta ctcaacaaaa
      241 ggttgagcaa gttgtaaaag atagtggact taaagcggat caaattcaaa tcaatggtaa
      301 agataataaa gtagcaactg ttcaatttaa agacgattta acgcgtgctc aagataataa
      361 attgagtgat aatattaaat cgaaattcgg tgacacacct caaattaata ctgtatcacc
      421 aattattgga caggaattag caaaaaatgc aatgcttgca ttaatctatg catcgatagg
      481 tatcatcatc tatgtatcat tacgatttga atggcgcatg ggtctttcat ctgtattggc
      541 attattacat gatgtattca ttatagtagc gattttcagt ttatttagaa ttgaagtaga
      601 tttaacattt atcgccgctg tattaacaat tgtcggttat tcaattaatg atacaatcgt
      661 aacgtttgac cgtgtacgtg aaaacttaca aaaggttaaa gtgattacga caacagaaca
      721 aattgatgat atcgttaata gatcaattag acagacaatg acacgttcaa ttaatacagt
      781 attaacagtt attgtagtag tagttgctat actattcttc ggtgctccta cgatattcaa
      841 ctttacttta gcattattta tcggattgat ttctggtgta ttctcttcaa tcttcattgc
      901 cgttccgcta tggggaataa tgaaaaaacg tcagctgaaa aaatcgccga aacacaaatt
      961 agttgtatat aaagaaaaga aatcgaacga tgaaaagatt ttagtttaaa atgaattaag
     1021 cggtatgtga aacaataaag agttcacata ccgcttttta ctttgtttaa atttaacaaa
     1081 atatatataa ataagttccc gcattcattt ttaaaaaatg gtataattaa agtcttgatt
     1141 atttagataa gtgcttttta tttaaagtaa ttttagatat aatgtctttt ggaaatgagg
     1201 cgtaaatata tatgattaaa ccgaagtata agtggaaatt aactaaacca gctgaataca
     1261 taagtgatga attaacatca aaattaaagt taacgccaat cgtaaaaaaa atattagaaa
     1321 gtaaatcgat tattgatgaa caaacaatag aagccatcat ctcagataca gatatcaatc
     1381 atgatgcact gcaattgagc gatatgacta agactattga aagaattaaa agagcgatag
     1441 cgaatgatga gaaaatccta gtttatggtg attacgatgc cgacggtgtt acctcaacta
     1501 caattttagt gacaacatta caacttttag gtgcacaagt aggatggcat attcctaacc
     1561 gctttaccga aggatatgga ccaaacgaac tagcatttag aaatgctcat gatgaaggca
     1621 taacattaat cataactgtt gataatggca tacaaggtca taatgaaatt aaaatggttc
     1681 aagatttagg agttgatgtc attgtaactg atcatcatga aattggtagt acattaccag
     1741 aagcatatgc aatcgtacat ccaatgcatc catcgtttaa ttatccgttc caacaattgt
     1801 gtggtgcagg tgttgcgtac aaattagcac aagcattgat tgaaaatgta cctgattatt
     1861 ttaaagcatt agttgcgata ggtaccattg cggatttagt atccttaact gatgaaaatc
     1921 gatcattagt gaaacaagga ttaaaagtct taaatgatca atgtccgacc tctgttaaag
     1981 cgttgctaaa agaagcggga tacaatgaca atattgatga agaaacaatt ggttttatta
     2041 ttggacctag acttaatgct gtaggtcgtt tagatgacgc atcattagct tgcgaattgt
     2101 tgatgactga cgacgaagaa gaagctgcat ttttagcaga acaagttgaa cattttaacc
     2161 gtgaaagaaa agatattgta gcaactatca cagaagaagc aatggctatg gcagaaacga
     2221 aagttaaaaa gggagattta tttttacttt tagcaaaaga aaattggcat gaaggtgtct
     2281 taggtattgt tgcttcgaaa attgtcgaaa catttgcttt gccaacatta attttaaata
     2341 ttgataggga gcaaaatcat gcaaaaggtt ccgccagatc aattgatcaa gtgtcgatgt
     2401 ttgaaatact atcagcacat caagagttaa tcgctaaatt tggcggccat catatggctg
     2461 caggtatgac gatggatatt gaaaatatag aatcgttagc tgaaggttta aataaatgga
     2521 tgaaagaatt atctaaaaca acttcgttag atcccgtaaa gccagtagac gtattattaa
     2581 cagaaaatga tataacgatt aaaaatattc gtgatatgaa tcgtttaaga cccttcggaa
     2641 cagatttttc tagacctatt tttgaaatgg atgatttgtc agtttcttca gttaaggcga
     2701 tcggtcaaca aaaaaatcat ctaaaattga cacttggtga aagtaatatc gccgcgctat
     2761 tttggcaaaa tggtcactta gaacctgagt tacaagatga acagccaatt aatatattag
     2821 gatctgttca aatcaacgaa tggaatggta atcaatcacc gcaattaatt attcaagata
     2881 ttgcgatgaa tgaacagcaa atattagatt atagaagtaa gcgaaaaagt ttacctttta
     2941 cagaaaatga tgaaaatatt gtcgtgctta ttcatcctaa aagtgataaa gtaaatgcga
     3001 atgaatatta ttatggtgaa gaaattaaac atcaaactga taaagtagta ttaagagatt
     3061 taccaacgtc aatggaagac ttgtctaatt ccttgcaaca actgcaattt tctcaacttt
     3121 atatagtttt gcaacataat cattcgattt acttcgatgg tatacctaat atggatattt
     3181 ttaaaaagtg ttataaagca ttaataacta aacaagaaac aaatatccag aaagagggta
     3241 tgttattgtg tcaacattta agtgtgaaac cagatacact taaattcatg ttgaaagttt
     3301 tcttagattt aaaatttgta acacaagaag atggtttaat tcgaatcaat caacaacctg
     3361 ataaaagatc gattgattcc agcaaagtat atcaattaag acaacaacgt atggatgttg
     3421 aaaagcaatt attatatcaa gatttttcag aaataaaaaa ttggataaag tcacaattgt
     3481 cgtgagcaat ttaggaggaa atattaatgg atttaaagca atacgtatca gaagttcaag
     3541 attggccgaa accaggtgtt agtttcaagg atattactac aattatggat aatggtgaag
     3601 catatggcta tgcaacagat aaaattgtag aatacgcaaa agacagagat gttgatatcg
     3661 ttgtaggacc tgaagcgcgt ggctttatca ttggctgtcc tgtagcttat tcaatgggga
     3721 ttggctttgc acctgttaga aaagaaggga aattacctcg tgaagtcatt cgttatgagt
     3781 atgacctaga atatggtaca aatgttttaa caatgcacaa agatgcaatt aaaccaggtc
     3841 aacgtgtgtt aattacagat gatttattag ctactggtgg tacgattgaa gcagcaataa
     3901 aattagttga aaaattaggc ggtatcgtag taggtattgc atttataatt gaattgaaat
     3961 atttaaatgg tattgaaaaa
//
LOCUS       DAHHOJ010000157         3940 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.159, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000157 DAHHOJ010000000
VERSION     DAHHOJ010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3940
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.159"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            61..2670
                     /gene="clpB"
                     /locus_tag="NBH32_002494"
     CDS             61..2670
                     /gene="clpB"
                     /locus_tag="NBH32_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCD1516062.1"
                     /translation="MDINKMTYAVQNALQQAVELSQQHKLQNIEIEAILSAALNESES
                     LYKSILERANIEVDQLNKAYEDKLNTYASVEGDNIQYGQYISQQANQLITKAESYMKE
                     YEDEYISMEHILRSAMDIDQTTKHYINNKVEVIKEIIKKVRGGNHVTSQNPEVNYEAL
                     AKYGRDLVEEVRQGKMDPVIGRDEEIRNTIRILSRKTKNNPVLIGEPGVGKTAIVEGL
                     AQRIVKKDVPESLLDKTVFELDLSALVAGAKYRGEFEERLKAVLKEVKESDGRIILFI
                     DEIHMLVGAGKTDGAMDAGNMLKPMLARGELHCIGATTLNEYREYIEKDSALERRFQK
                     VAVSEPDVEDTISILRGLKERYEVYHGVRIQDRALVAAAELSDRYITDRFLPDKAIDL
                     VDQACATIRTEMGSNPTELDQVNRRVMQLEIEESALKNESDNASKQRLQELQEELANE
                     KEKQAALQSRVESEKEKIANLQEKRAQLDESRQALEDAQTNNNLEKAAELQYGTIPQL
                     EKELRELEDNFQDEQGEDTDRMIREVVTDEEIGDIVSQWTGIPVSKLVETEREKLLHL
                     SDILHKRVVGQDKAVDLVSDAVVRARAGIKDPNRPIGSFLFLGPTGVGKTELAKSLAA
                     SLFDSEKHMIRIDMSEYMEKHAVSRLIGAPPGYIGHDEGGQLTEAVRRNPYSVILLDE
                     VEKAHTDVFNVLLQILDEGRLTDSKGRSVDFKNTIIIMTSNIGSQVLLENVKETGEIT
                     ESTEKAVMTSLNAYFKPEILNRMDDIVLFKPLSIDDMSMIVDKILTQLNIRLLEQRIS
                     IEVSDDAKAWLGQEAYEPQYGARPLKRFVQRQIETPLARMMIKEGFPEGTTIKVNLNS
                     DNNLTFNVEKIHE"
     gene            complement(2729..3598)
                     /locus_tag="NBH32_002495"
     CDS             complement(2729..3598)
                     /locus_tag="NBH32_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCD1516063.1"
                     /translation="MNLDWYYTFVILAKTLNYRLASEEINLTIPSIHKQIKNLEQHLN
                     VKLFETYKNQIILTEDGHTFLPIAQSFIEQYESGIKHIQLKKTMFQSKLNVVVSSYIA
                     TFIMPKFLKSFFNEHPFIDVSLHVKNENIEKDINNHTYDIGISRNQPKLREVHSEKVC
                     EGKIVLIAPNKENNHLLTEASLFEKYKIISDNHPEYWSSLKNNILNIYEKAQFLSIND
                     VHTSIKLIEMNQGISFLPIYITTDSDYNISVINTKILQAPISFTYIYSKKESPEILAF
                     IRSFKKYIANEQL"
     gene            3708..>3940
                     /locus_tag="NBH32_002496"
     CDS             3708..>3940
                     /locus_tag="NBH32_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCD1516064.1"
                     /translation="MIRIQDNTIRDGMQQSNVRKSIIVKKEVLQEINKISINSVEVGM
                     CTTFEDEYNIHQYREILNNDKEIVVLTRLDENE"
BASE COUNT         1489 a          515 c          735 g         1201 t
ORIGIN      
        1 aaagaaagtc aaagtcaaaa gtgcaacttg accaactcta caaattagag gtgaaattat
       61 atggatataa ataaaatgac atatgctgtt caaaatgctt tacaacaagc agttgaactg
      121 agtcagcaac ataaattaca aaatatagaa attgaggcaa ttttaagcgc tgccttaaat
      181 gaaagtgaaa gcttatataa aagtatttta gaacgagcaa atattgaggt agatcaatta
      241 aacaaagctt atgaagacaa actaaacacg tatgcatctg tagaaggtga caatatacaa
      301 tatggtcaat atattagcca acaagcaaac caattgataa ctaaggctga atcatacatg
      361 aaagaatatg aagatgaata tatttcaatg gagcatattt tacgttcggc aatggacatt
      421 gatcaaacaa caaaacatta tataaataat aaagtagaag ttatcaaaga aattattaaa
      481 aaagtaagag ggggaaatca cgtgacatca caaaatccag aagttaatta cgaagcatta
      541 gctaaatatg gccgcgactt agtagaagaa gttagacaag gtaaaatgga tcctgttata
      601 ggaagagatg aagaaattcg aaatacgatt cgtattttaa gtcgtaaaac taaaaacaac
      661 cctgtgctca ttggtgaacc aggtgttggt aaaactgcaa ttgttgaagg attagcgcag
      721 cgtatagtta agaaagatgt gccagaatca ttattagata aaactgtttt tgagttagat
      781 ttaagcgcat tagtagcggg cgctaaatat cgtggtgaat ttgaagagag attaaaagca
      841 gtcctaaaag aagttaaaga gtctgatggt agaattatat tatttattga tgaaatccat
      901 atgcttgttg gtgctggtaa aacagatggt gccatggatg caggcaacat gctaaaacca
      961 atgttagcac gaggagagtt acattgtatt ggtgcaacaa ctttaaatga atatcgagaa
     1021 tatattgaaa aagattcggc attagagcgt cgtttccaaa aagtagcagt tagtgagcct
     1081 gatgttgaag atacaatttc aattttacgt ggtttaaaag aacgatatga agtgtatcat
     1141 ggtgtgcgta ttcaagatag agccttagtt gctgccgctg aattgtctga tcgttacatc
     1201 actgatcgtt ttttaccaga taaagcgatt gatttagttg accaagcatg tgcaacaatt
     1261 cgtacggaaa tgggatcaaa tccaactgaa ttggatcaag ttaatagacg tgtcatgcaa
     1321 ttagaaattg aagaaagcgc acttaaaaat gaatctgaca atgcgagcaa acagagatta
     1381 caagaactac aagaagagct tgccaatgaa aaagagaaac aagcagcact tcaatctcgt
     1441 gtagaatcag aaaaagaaaa aatagcaaat ttacaagaaa aacgtgcgca actagatgaa
     1501 agtagacaag cgttggaaga tgcacaaaca aataacaatt tagaaaaagc tgctgaacta
     1561 caatatggaa caattcctca attggaaaaa gaacttagag aattagagga taatttccaa
     1621 gatgagcaag gtgaagatac agatcgaatg attcgtgaag ttgtaacaga cgaagaaatt
     1681 ggcgatattg tcagccaatg gacaggcata ccagtttcaa aattagttga aacagaacgt
     1741 gaaaaattac ttcacttaag tgacatcttg cataaacgtg ttgtaggtca agataaagcg
     1801 gttgacctgg tttcagatgc agtagttaga gcaagagcag gtattaaaga tccaaacaga
     1861 cctattggta gtttcttatt cctaggtcca actggagtag gtaaaactga attagctaaa
     1921 tcattagctg catcattatt tgattctgaa aaacatatga ttcgtattga tatgagtgaa
     1981 tatatggaaa aacatgcagt atcaagattg ataggggcac ctccaggata tattggacat
     2041 gatgaagggg gtcaattaac tgaagcggtt cgtcgtaatc catactcagt tattttatta
     2101 gatgaggttg aaaaagcgca tactgacgtc tttaatgtat tattgcaaat tttagatgaa
     2161 ggtcgtttaa ctgattctaa aggacgtagc gttgatttta aaaatactat tattattatg
     2221 acaagtaata ttggatctca agttttatta gaaaacgtaa aagagactgg tgaaattaca
     2281 gaatcaacag aaaaagctgt tatgacaagt ttaaatgcat atttcaaacc agaaattttg
     2341 aatcgtatgg atgatatcgt attatttaaa ccattatcta ttgatgacat gagtatgatt
     2401 gtagataaaa tcttaacgca attaaatata agattactag aacaacgaat ctcaattgaa
     2461 gtttctgatg atgctaaagc ttggctaggt caagaggctt atgaacctca atacggtgca
     2521 agaccattaa aacgttttgt acaacgccaa attgaaacac cattagcacg tatgatgatt
     2581 aaagagggat tcccagaagg tacaacgatt aaagttaatt taaattcaga caataactta
     2641 acgtttaatg ttgaaaaaat tcatgaataa aattgaacca agaatgtgat gattaatcgc
     2701 attcttggtt ttttagattt gaaatttatt ataattgttc attggcaata tactttttaa
     2761 atgatctaat aaacgccaat atttctggac tttctttttt gctgtatata tatgtaaatg
     2821 aaataggtgc ttgtaaaatt ttcgtgttaa taaccgaaat attgtaatca ctatctgttg
     2881 ttatataaat aggcaggaat gatatacctt gattcatttc gattaattta attgaagtat
     2941 gcacatcatt gatagataga aattgtgctt tttcataaat atttaaaata ttatttttaa
     3001 gtgatgacca atattctgga tggttatcac ttattatttt gtatttttca aataaagatg
     3061 cctcggttag aagatgatta ttctctttat tgggagcaat caatacaatt ttaccttcgc
     3121 atactttttc agaatgaact tctcttagtt taggttgatt tctgctaatc ccgatgtcat
     3181 acgtatgatt attaatatct ttttcaatat tttcattttt gacatggaga gaaacatcaa
     3241 taaacggatg ctcgttaaag aaagatttta aaaatttggg cataatgaat gtcgcgatat
     3301 atgaagacac gacaacattt aatttcgatt gaaacatcgt ctttttaagt tggatatgct
     3361 tgatgccact ttcatattgt tcaataaaac tttgcgcaat tggaagaaat gtatgaccat
     3421 cttcagtcaa gataatttga tttttataag tttcaaatag tttcacattg agatgctgtt
     3481 ctaaattttt aatttgctta tgtatagaag gtatagtgag attaatttct tcactagcta
     3541 atcgatagtt taacgtcttc gctaaaatga caaatgtata gtaccaatcc aaattcatga
     3601 acttacctct ttcaaaaaag ttaataattc attagataat atttaatttt tattgcataa
     3661 aaataatatt aatattatat cagaggttac cgagggggtg aagtaatatg attcgaattc
     3721 aagataatac aataagggat ggtatgcaac aaagtaatgt tcgtaaaagc attattgtaa
     3781 aaaaagaagt gttacaagaa ataaataaga taagcataaa ttctgtggaa gttggcatgt
     3841 gcacaacttt tgaggatgaa tataatattc atcaataccg agaaatttta aataatgata
     3901 aagaaatagt agtcttaact cggctagatg aaaatgaaat
//
LOCUS       DAHHOJ010000158         3873 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.160, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000158 DAHHOJ010000000
VERSION     DAHHOJ010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.160"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..758
                     /gene="gcvT"
                     /locus_tag="NBH32_002497"
     CDS             <1..758
                     /gene="gcvT"
                     /locus_tag="NBH32_002497"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD1516065.1"
                     /translation="LVVNAANTEKDFNWILKHKEKFDVEVQNVSNQYGQLAIQGPKAR
                     DLINQLVDEDVTEMKMFEFKQGVKLFGANVILSQSGYTGEDGFEIYCNIDDTEKIWDG
                     LLEYNVMPCGLGARDTLRLEAGLPLHGQDLTESITPYEGGIAFASKPLIDADFIGKSV
                     LKDQKENGAPRRTVGLELLEKGIARTGYEVMDLDGNIIGEVTSGTQSPSSGKSIALAM
                     IKRDEFEMGKELLVQVRKRQLKAKIVKKNQIDK"
     gene            778..2124
                     /gene="gcvPA"
                     /locus_tag="NBH32_002498"
     CDS             778..2124
                     /gene="gcvPA"
                     /locus_tag="NBH32_002498"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HCD1516066.1"
                     /translation="MSHRYIPLTEKDKQEMLQTIGAKSIGELFGDVPSDILLNRDLNI
                     AEGEAETTLLRRLNRIASKNITKETHTSFLGAGVYDHYAPSVVDAMISRSEFYTAYTP
                     YQPEISQGELQAIFEFQTLICELTDMDVANSSMYDGMTSFAEACILAFSQTKKNKIVV
                     SKGLHYQALQVLHTYAKTRKEFEVVEIDLDGTVTDLKKLEAAVDDETAAVAVQYPNFY
                     GSIEDLEKIHSFIEDKKALFIVYANPLALGLLTPPGSFGADIVVGDTQPFGIPAQFGG
                     PHCGYFATTKKLMRKVPGRLVGQTQDDEGNRGFVLTLQAREQHIRRDKATSNICSNQA
                     LNALASSIAMSALGRQGIYDIAVQNIEHANYAKQQFIKKGFEVLDGTSFNEFVVKFDK
                     PIQQVNEELVKYNIIGGFDLGVVSDDFKNHMLIAVTELRTKDEIDTFVEKAGELND"
     gene            2117..3589
                     /gene="gcvPB"
                     /locus_tag="NBH32_002499"
     CDS             2117..3589
                     /gene="gcvPB"
                     /locus_tag="NBH32_002499"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HCD1516067.1"
                     /translation="MTSKSSPLIFERSREGRYAYSLPKSDIKTNSVESLLDDKFIRKN
                     KAEFPEVAELDLVRHYTELSNKNFGVDNGFYPLGSCTMKYNPKINEKVARIPGFSESH
                     PLQDEDQVQGSLEIIYSLQEELKEITGMDEVTLQPAAGAHGEWTALMIFKAYHENNGE
                     GHRDEVIVPDSAHGTNPASASFAGFKSVTVKSNERGEVDIDDLKRVVNENTAAIMLTN
                     PNTLGIFEKNIMEIREIVHNAGGLLYYDGANLNAIMDKVRPGDMGFDAVHLNLHKTFT
                     GPHGGGGPGSGPVGVVKELASYLPKPMVIKDGNKFKYDNDIKNSIGRVKPFYGNFGIY
                     LRAYTYIRTMGATGLKEVSEAAVLNANYIKARLSEHFEIPYKQYCKHEFVLSGVRQKE
                     FGVRTLDMAKRLLDFGVHPPTIYFPLNVEEGMMIEPTETESKETLDYFIDTLISIAEE
                     AKNDPDKVLEAPHTTVIDRLDEATAARKPILKFENLKQEK"
BASE COUNT         1397 a          527 c          757 g         1192 t
ORIGIN      
        1 tattagttgt taatgctgct aatactgaaa aagattttaa ttggatttta aaacacaaag
       61 agaaatttga tgttgaagta caaaatgtat caaaccaata tggtcaatta gcaatacaag
      121 gaccaaaagc tagagattta attaatcaat tagttgatga agatgtaact gaaatgaaaa
      181 tgtttgaatt taaacagggt gtcaaattat ttggagcaaa cgtcatttta tctcagtcag
      241 gttacacagg tgaagatggg tttgaaattt attgtaatat cgatgatact gaaaaaattt
      301 gggatggttt attagagtat aatgttatgc catgtggatt aggcgctcgt gatacattaa
      361 gattagaggc tggattgcca ttacatggtc aagatttaac tgaatcaatt acaccatatg
      421 aaggtggtat cgcttttgca agtaaaccat taattgatgc tgattttatt ggtaaatctg
      481 tattaaaaga tcaaaaagaa aatggtgcac caagaagaac agtgggatta gaattacttg
      541 aaaaaggaat tgcaagaact ggttatgaag ttatggattt agatggaaat attattggag
      601 aagtaacttc aggaacacag tctccatcat caggaaaatc aattgcactt gcaatgataa
      661 aaagagatga gtttgaaatg ggtaaagagt tgcttgttca agttcgtaag cgtcaattaa
      721 aagcgaaaat tgttaagaaa aatcaaattg ataaataatt aaaaaggggt gtgcattgtg
      781 agtcatcgtt atataccttt aactgaaaaa gacaagcaag aaatgttaca aacaattggt
      841 gcaaaatcta taggagaatt attcggtgat gtaccaagtg acattttatt aaatagagat
      901 ttaaatattg ctgaaggcga agcggaaaca acgttactta gaagattaaa tcgcattgca
      961 agcaagaata tcactaaaga aacgcataca tcatttttgg gtgctggtgt ttatgatcat
     1021 tacgcgccat cagttgtgga tgctatgatt tcaagatcag aattttacac agcatatacg
     1081 ccatatcaac cggaaatttc tcaaggtgaa ttacaagcca tttttgagtt ccaaacttta
     1141 atttgtgaat taacagatat ggatgtagca aattcttcta tgtatgatgg gatgactagc
     1201 tttgctgaag catgtatttt agcatttagt caaacaaaga aaaataaaat tgtagtctca
     1261 aaaggtttac attatcaagc attacaagta cttcatactt atgctaagac acgcaaggaa
     1321 ttcgaagtag tggaaatcga tttagatggc actgttacag atttaaaaaa attagaagca
     1381 gcagtggatg atgaaacagc agctgttgca gtacaatatc caaacttcta cggttcaatt
     1441 gaagatcttg aaaagattca tagctttatt gaagataaaa aagcattatt tatcgtatat
     1501 gcaaatccat tagcattggg attacttaca cctccaggtt catttggtgc agatattgtt
     1561 gtaggtgata ctcagccatt cggaattcct gctcaattcg gtgggccaca ttgtgggtat
     1621 tttgcaacga cgaaaaagtt aatgcgtaaa gtgccaggta gattagtcgg tcaaacacaa
     1681 gatgatgaag gtaatcgtgg ctttgtatta acactacaag cacgtgaaca acatattcga
     1741 cgcgataaag cgacatctaa tatttgttct aaccaagcat taaatgctct agcgtcatca
     1801 attgcaatgt cagcacttgg tagacaaggt atctatgata ttgcagttca aaatatagaa
     1861 catgctaact atgctaaaca acaatttatt aaaaaaggtt ttgaagtatt agatggtaca
     1921 tctttcaatg agtttgtagt caaatttgac aaaccaattc aacaagttaa cgaggaatta
     1981 gtgaagtaca atattatcgg tggttttgat ttaggtgttg tttcagacga ctttaaaaat
     2041 catatgttaa tagctgtaac tgaattaaga acaaaagatg aaatcgatac atttgtagaa
     2101 aaggctggtg agttaaatga ctagtaaatc aagtccatta atttttgaaa gatctcgtga
     2161 aggcagatat gcatattcat taccaaaaag tgatattaaa acaaattctg ttgagtcatt
     2221 gttagatgat aaatttattc gtaaaaacaa agcggagttt ccagaagttg ctgaactaga
     2281 cttagtacgt cattatactg aactttcaaa taaaaatttc ggtgtagata acggatttta
     2341 tccattgggt tcatgtacga tgaagtataa ccctaaaatt aatgaaaagg ttgctagaat
     2401 accaggattc agtgaatcac atccattaca agatgaagac caagtacaag gttcattaga
     2461 aattatttat agtttgcaag aagaattaaa agaaattact ggtatggatg aggtaacatt
     2521 acaaccagct gctggtgcac atggtgaatg gactgcattg atgatattta aagcttatca
     2581 tgagaataat ggtgaaggtc atcgtgatga agtcattgtg ccagattctg cgcatggtac
     2641 gaatccagcc tcagcttcat ttgcaggatt taaatcagtt actgtaaaat caaacgaacg
     2701 tggcgaagtt gatattgatg acttgaaacg tgttgtaaat gaaaatacag cagctattat
     2761 gttaactaat ccaaacactt taggtatttt cgaaaaaaat attatggaaa tccgtgaaat
     2821 cgtccataat gctggtggtc tattatatta tgatggtgcg aatttaaacg ctattatgga
     2881 caaagttcgc ccaggagata tgggatttga tgctgttcat ttaaacttgc ataaaacatt
     2941 tactggtcca catggtggtg gcggtcctgg ttcaggtcca gtcggtgtag taaaagaact
     3001 agcaagttac ttaccaaagc caatggttat taaagatggc aacaaattta aatatgataa
     3061 tgacattaaa aattctatcg gacgtgtaaa accattttat ggtaactttg gtatttactt
     3121 aagagcttat acgtatatta gaactatggg agcaactgga cttaaagagg tttctgaagc
     3181 agcggttctt aatgcgaatt atattaaagc acgtttatct gaacactttg aaatacctta
     3241 taaacaatat tgtaaacacg agtttgtgtt aagtggtgtg cgtcaaaaag aatttggtgt
     3301 acgtacttta gacatggcta agcgattatt agatttcggt gtacatccac caacaatcta
     3361 cttcccatta aatgttgaag aaggtatgat gattgaaccg actgagacag agtctaaaga
     3421 aacacttgat tattttatcg atacattaat tagtattgct gaagaagcta aaaatgatcc
     3481 tgataaagtg ttagaagcac cacatacaac tgtgattgat cgattagacg aagctacagc
     3541 tgctcgtaaa ccaatattaa aatttgaaaa tcttaaacag gaaaaataaa gtattaaaca
     3601 catattccga gaattattat tctaactttg tatgaagatt taaggataat ggtttcaaaa
     3661 tcaattgaaa aagacaattt ctatttgaac aagaaaacta aaccgaagta ataactctta
     3721 gggtttggta ttattctttc atagaaattg tcttttcatt ttttagattg cggtaattga
     3781 atcgtattga aaatgagctg aactttcttt attatgctga aactaagttt aatgatcagt
     3841 gaagctagag aaatatagta acagactttg tta
//
LOCUS       DAHHOJ010000159         3761 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.162, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000159 DAHHOJ010000000
VERSION     DAHHOJ010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.162"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            145..348
                     /locus_tag="NBH32_002500"
     CDS             145..348
                     /locus_tag="NBH32_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516068.1"
                     /translation="MQLSHSVKVAISIYLALIFITFTSYLVIILYTSMTGHDVSHFVL
                     DSQHSHHGSLTQKHLSLPEISFK"
     gene            531..1466
                     /locus_tag="NBH32_002501"
     CDS             531..1466
                     /locus_tag="NBH32_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HCD1516069.1"
                     /translation="MRYLANNHSALRLKGIILAIVGACLWGLGGTVSDFLFKYKNINV
                     DWYVTARLVVSGVFLLIMYKMMQPKRSIFSVFQDRRMLGKLLIFSILGMLVVQYAYMA
                     SINTGNAAIATLLQYIAPVYIIIWFVIRGVAKLTLFDVLAIIMTLLGTFLLLTNGSFS
                     NLVVNPASLFWGILAGVALAFYTIYPSDLLNRFGSILIVGWAMLISGVAMNLRHPIWH
                     IDITKWDISIILFLIFGIIGGTALAFYFFIDSLQYISAKETTLFGTVEPVVAVIASSL
                     WLHVAFKPFQIVGIILIMILILLLSLKRQPEALDE"
     gene            complement(1548..2516)
                     /locus_tag="NBH32_002502"
     CDS             complement(1548..2516)
                     /locus_tag="NBH32_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1516070.1"
                     /translation="MRKKWSTLAFGFLVAAYAHIRIKEKRSVKSYMLEQGIRLSRAKR
                     RFMYKEEAMKALEKMAPQTAGEYEGTNYQFKMPVTVDKHYGSTVYTVNDKQDKHQRVI
                     LYAHGGAWFQDPLKIHFEFIDELAETLNAKVIMPVYPKIPHQDYQATYVLFEKLYHDL
                     LNQVADSKQIVVMGDSAGGQIALSFAQLLKEKHIVQPGHIVLISPVLDATMQHPEIPD
                     YLKKDPMVGVDGSVFLAEQWAGDTPLDNYKVSPINGDLDGLGRITLTVGTKEVLHPDA
                     LNLSQLLSAKGIEHDFIPGYYQFHIYPVFPIPERRRFLYQVKNIIN"
     gene            complement(2637..2993)
                     /locus_tag="NBH32_002503"
     CDS             complement(2637..2993)
                     /locus_tag="NBH32_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000240663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HCD1516071.1"
                     /translation="MVKQLNSVEAFREFIHQYPLAVVHVMRDQCSVCHAVLPQIEDLM
                     QSYPNVPLAVINQSQVEAIAGELNIFTVPVDLIFMNGKEMHRQGRFIDMQRFEHHLKQ
                     MNDSVNNDVDEHEYRK"
     gene            complement(3035..3445)
                     /locus_tag="NBH32_002504"
     CDS             complement(3035..3445)
                     /locus_tag="NBH32_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HCD1516072.1"
                     /translation="MTTEGLLVAEKEIEVNGYDIDAMGVVSNIVYIRWFEDLRTAFIN
                     QHMNYSTMINQGISPILMKTEAEYKVPVTIHDKPVGRIYLVKASKMKWVFQFEIVSAH
                     GVHCIGTQTGGFYRLSDKKITSVPQVFQDILATK"
BASE COUNT         1124 a          689 c          589 g         1359 t
ORIGIN      
        1 attattttta gttttctgat aggaatgatt atagtctata ggttgaatct ttactttttt
       61 taaagctaaa tttacatcaa cttaacaatg gctgtttata ctgaagatga aaatatttag
      121 tataacttag tggaggcgat aaagatgcaa ttgagtcatt ccgttaaagt tgcaatttct
      181 atctatttag cacttatctt tataacgttc acttcttatt tagtcattat tttatatacg
      241 agtatgactg gacatgatgt atcacatttc gttttagata gtcagcattc tcatcatgga
      301 tctcttacgc aaaaacattt gagtcttcct gaaatctcat ttaaatagtt tatcctctgt
      361 gtttcaacat tcatttccca tatcgattca tttatctatc atctagacca ctacatctta
      421 gatgattttt ttattttctc atttcactct ttcttaaaag tcgatataat gaattaaatc
      481 attatcatac accgacatat tttatgttgt tggtgttaag ttttaaaggg gtgagatact
      541 tggcgaacaa tcattcagct ttgcgtttaa aaggaattat acttgccatt gtcggtgctt
      601 gtttatgggg attaggtggt actgtttctg atttcttgtt caaatataag aatattaatg
      661 tcgattggta cgtcactgct cgacttgtag tcagtggtgt tttcttactt attatgtaca
      721 aaatgatgca acccaaacgt tcaatattta gcgtattcca agatcgacgt atgttaggca
      781 aattacttat cttcagtata ctgggcatgt tagttgtaca atatgcttat atggcatcta
      841 ttaatacagg taatgctgcg attgcaacat tactacaata cattgcgcca gtttatatta
      901 ttatttggtt tgtcataaga ggcgttgcaa aactaacatt atttgatgtg cttgctatta
      961 tcatgacact attaggaaca tttttattat taacgaatgg ttcattttct aatttagtcg
     1021 tcaatcctgc aagtttattc tggggtattt tagctggtgt agcactcgct ttttacacaa
     1081 tttatccttc agacctactt aaccgcttcg gttcgattct aattgtcggg tgggcaatgc
     1141 ttatttctgg tgttgcgatg aatttacgcc atccaatttg gcacattgat atcactaaat
     1201 gggacatatc aattatatta tttttaatct ttggtattat cggtggtacc gcactcgcat
     1261 tttatttctt tatcgacagt ttacaataca tatcagcgaa agaaacaaca ttattcggaa
     1321 ctgttgaacc tgtcgtagcc gttatcgcaa gcagtctatg gttacatgtg gcattcaaac
     1381 catttcaaat cgtaggcatc attcttatta tgattttaat tttattacta tcacttaaaa
     1441 gacaacctga agcattagat gaataagaaa actctgataa tcactttagc aagtaactat
     1501 tatttaacaa tgtagctacc ttgtaggtga tatcagagtt ttttatttta attaataata
     1561 tttttcactt ggtataaaaa gcgtcggcgc tctggtatcg gaaatactgg ataaatatgg
     1621 aattggtaat atccaggtat aaagtcatgt tcgatacctt tcgcactcaa caattgcgat
     1681 aagttcaaag catctgggtg cagtacttct tttgtgccaa cagttaatgt aatacgacct
     1741 aaaccatcta agtcaccatt aattggtgat actttgtagt tatctaaagg cgtgtcccct
     1801 gcccattgtt cagctaagaa cacactgcca tccacaccta ccattgggtc tttctttaag
     1861 tagtcaggaa tttcaggatg ctgcatcgtt gcatctaaaa ctggtgaaat taatacaata
     1921 tgtcctggtt gcacaatatg tttttctttt aacaattgag caaatgataa agcaatttga
     1981 ccgcccgcag agtcacccat tacaacgatt tgtttagaat ctgctacttg attcaataaa
     2041 tcatggtaca acttttcaaa aagtacatac gtcgcttgat aatcttgatg cggaatcttc
     2101 ggatatactg gcatgatgac tttagcattg agtgtttctg caagttcatc aataaattcg
     2161 aaatgaattt tgagtgggtc ttggaaccat gcccctccat gtgcatataa tataacgcgt
     2221 tgatgcttat cttgtttatc gttaacggta taaacggttg aaccgtagtg cttatccact
     2281 gttactggca tcttaaactg ataattggtg ccctcatatt cacctgctgt ctgtggcgcc
     2341 attttttcta atgctttcat cgcttcttct ttatacataa aacgacgctt agctctagat
     2401 aatcgtatac cttgttctaa catataactt ttcacactgc gtttttcttt aattctaata
     2461 tgtgcgtatg ctgctactaa aaatccaaac gcaagtgtag accatttttt acgcatttgt
     2521 cgtcactcct ctttgatgat ttctactata gtattaatgt accatgctga ttcttattac
     2581 taccaatatt tactgggaaa taagttatga tgtctacata atcttagcaa tgctaatcat
     2641 ttgcgatatt catgctcatc gacatcgtta ttcacactat cattcatttg cttaagatga
     2701 tgttcaaaac gttgcatatc gataaaacgc ccttgacgat gcatttcttt tccattcata
     2761 aaaattaaat ccacaggtac agtgaaaata tttaattctc cagcaatagc ttccacctga
     2821 ctttgattaa tcacagctaa tggcacattg ggatatgatt gcatcaagtc ttcaatttgt
     2881 ggtaaaacgg catgacacac gctacactga tcgcgcatga catgtacgac tgctaacgga
     2941 tattgatgaa taaattcacg gaatgcttcg acactattta attgcttaac catttcgaac
     3001 accttcttac tttttatatt ttaaaatgaa gtcattattt tgttgctaaa atgtcttgaa
     3061 acacttgtgg cacagaggtt atcttcttat cgctcaatct gtaaaaaccg cctgtctgtg
     3121 taccaataca atgcacgcca tgtgcggaca caatttcaaa ctgaaacacc catttcatct
     3181 tgcttgcttt aactaagtaa atacgaccta ctggtttgtc atgtattgtg acaggtactt
     3241 tatactctgc ttccgttttc ataagtatag gtgaaatgcc ttgattgatc attgttgagt
     3301 aattcatgtg ctgattaata aatgctgttc tcaaatcttc gaaccatcta atataaacga
     3361 tattactaac gacacccatc gcatcaatgt cgtaaccatt cacttcgatt tctttctctg
     3421 caactaatag accttctgtt gtcataatga caaatctcct tatcttacaa atagatttaa
     3481 aatcatgtta atttattttt attactttca tgataaaaat acaagcaatt gaactttatt
     3541 acggaaaatc aaacttttca atgagactat taatttggct agtcgcgaag atgcattttt
     3601 ctaaaatgtg acaaaaacat aagcaatgtt ttctatatct aacactaata tttaacttat
     3661 tatcatcaac acatctgcta tacatcattg cgcactaaaa acaggctgga acataaatcc
     3721 ctaaaaaaac agcagtaaga tattttctaa ttgaaaatta t
//
LOCUS       DAHHOJ010000160         3761 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.163, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000160 DAHHOJ010000000
VERSION     DAHHOJ010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3761
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.163"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..547
                     /locus_tag="NBH32_002505"
     CDS             <1..547
                     /locus_tag="NBH32_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1516073.1"
                     /translation="NITEKKEVKVKKKKKSEKDKDEYETKTEEKQRKVNQNILINIPI
                     KSENNKYVVVEYPYFTPIPDSQLNKAKMVEDNLKDNKREDNPKAKAFIEDFFNKYASS
                     KSDDMAYLMDNPEGLEGTREVSQIREIRLYPKGDDYVAKVEILMKDKDSPLENLEHYT
                     LDITKKDGKYYVKNMTNSIGG"
     gene            552..812
                     /locus_tag="NBH32_002506"
     CDS             552..812
                     /locus_tag="NBH32_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000015642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516074.1"
                     /translation="MSGLFNFFVLGADRPSLGGVGDWISNEVGTGIGLVVLVVGIVKW
                     ASGKYGHMVILFVVGGFLFLVSKGPEQVFNAIAGVWKMIFGG"
     gene            824..1207
                     /locus_tag="NBH32_002507"
     CDS             824..1207
                     /locus_tag="NBH32_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015581841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1516075.1"
                     /translation="MDKKAYNLKRTFEQPIVMYEFTDKFRINKGFRLDFWATFLIVWF
                     ILFLLFWYLLQPVIMSIGGLMFIYFTVAPYYITKYIVKLKQDGKKLFFFLWDFVIFVF
                     NVQLRKVKLSYDEEVEYHDKKITFK"
     gene            1242..3737
                     /locus_tag="NBH32_002508"
     CDS             1242..3737
                     /locus_tag="NBH32_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCD1516076.1"
                     /translation="MNLKAPYAGLRDNLLLTKTGEVWAYYRVRSESISTANYDAKEES
                     KKRMRYFLESIKGYQDFHFEMYDHDLDLRNKFKEISKDFDDEAFNVAEYYSKETIDVL
                     EQELQMITHNSFVMGVKIRELADDAVTLKEILKSTLSDTAERIVSNFGFDVNLDDKIL
                     DKYKTFERDLADSFLGIGGERLTENELAYIIRKPFISNATHDVQEESSRRAITDIYNA
                     VIDPTRLSVLEVENDNEKFYTTTVVVDDFPLDMEYTHLFEKAQNMPFPVECHVKAKII
                     NNEKVKKKFDRAKINHKQQAKEKQDTGDSASEDVQDNLFVLNQMEREVTGSGVFIEWV
                     FMFVIKSDDYRDCKRKAKRLMKRLKETQIYAVNPLADQLQLFYQCLHGNDLFINKYWL
                     QRTTATGIAENLFGVSQSLGTKTGFYIGRIDKFIRSVSREEAVASSRDIILFSLLLAA
                     KGIKGAVSDSPHVLITGQTGKGKSFLAKLLWIYTSFFKGQMLYWDPKSEFAEWFDRVT
                     ESEEMQQKYPLFINHLKTFKYVTLNYEDSTNYGCLDPIVFLDGIEANEMLKSVFDEIK
                     SFENYHHIETAIYQAISDTVEERENGKKVGSLNVIEKLQNNEDEHVKNAGDLLFEKTQ
                     NNILKLVFSDGTNPALNIQEKATILQVKGLDMPKADDDTSSYSTSEKNGITLMLLIGK
                     FLEKFGSRRDVQTTIFIDEGWAFSASRQGKKVGKRIKRTGRSENNSLVFITQSVKDKA
                     DDDGGNFGCHFAFDEKDEREDILKSLGLEYSKESPENMEMLKDLKKGQCIFSDFYGRV
                     GKMVVHCPFEEMTEAFRTQEDSASSKAEEKFAM"
BASE COUNT         1359 a          379 c          759 g         1264 t
ORIGIN      
        1 gaatattact gaaaagaaag aagttaaagt taagaagaaa aagaaaagtg aaaaagataa
       61 agatgagtat gaaacaaaaa cagaagaaaa gcaacgtaaa gtaaatcaaa acattttaat
      121 aaatatacct attaagagtg aaaataataa gtatgttgtt gttgaatatc cttattttac
      181 gccaatccct gatagtcaat tgaataaagc taaaatggtt gaggataatt tgaaagacaa
      241 taaacgggaa gacaatccga aagcaaaagc ttttattgaa gatttcttta ataaatatgc
      301 ttctagtaag tctgatgata tggcctattt aatggataac cctgagggct tagaaggaac
      361 tagagaagtt tctcagataa gagaaattag gttatatcca aaaggcgatg attatgttgc
      421 taaagttgaa attttgatga aagataaaga ttctcctttg gagaatttgg agcattacac
      481 attagatata acaaaaaaag atggtaaata ttacgttaaa aatatgacta attctattgg
      541 aggttaatgt tatgagtggt ttatttaatt tttttgtttt aggtgctgac cgtccatcac
      601 ttggtggagt tggtgactgg ataagtaatg aagtaggtac gggtattggt ttagtagtat
      661 tggttgttgg tattgtaaaa tgggctagtg gtaaatatgg gcatatggtt atattatttg
      721 ttgttggtgg ctttttattc ttagtttcta aaggtcctga acaggtattt aatgcgattg
      781 caggtgtttg gaaaatgatt tttggcggtt gaggtgattc catttggata aaaaagctta
      841 taatttaaag cgaacttttg aacaaccgat agttatgtat gaatttacag ataagtttcg
      901 tattaataag ggttttcgtt tagatttttg ggctacattt ttgattgtgt ggtttatatt
      961 atttttgtta ttttggtatt tattacagcc tgtaattatg tcgattggtg gacttatgtt
     1021 tatttacttt acggttgcac cttattatat aacgaaatat attgttaagt taaaacaaga
     1081 tggaaagaaa ttatttttct tcttatggga ttttgtaata tttgttttta atgtgcaatt
     1141 aagaaaagtt aaattaagtt atgatgaaga agtagaatat catgataaaa aaataacatt
     1201 taaatagccc ctattggggt tatttttatt ggagggatga catgaattta aaagcaccgt
     1261 atgcaggttt aagagataat ttgttattaa cgaaaactgg cgaagtttgg gcgtattatc
     1321 gtgttcgttc tgagtctata tcaacagcaa attatgatgc aaaagaagaa tcaaaaaagc
     1381 gtatgcgtta ttttttagag tcgattaaag gttatcaaga ttttcatttt gaaatgtatg
     1441 accacgattt agatttgcga aataaattta aagaaattag taaagatttt gatgatgaag
     1501 cttttaatgt agctgaatat tattcaaaag aaactattga cgtattagaa caagaattac
     1561 aaatgattac gcataattct tttgttatgg gtgttaaaat acgtgaatta gctgacgatg
     1621 cagttacact taaagagatt ttaaaaagta cattatcaga tacggctgag cgtattgttt
     1681 cgaattttgg ttttgatgtg aatttagacg ataaaatttt agataagtat aaaacatttg
     1741 aacgagattt ggctgatagt tttctaggta ttggaggcga gcgtttaact gagaatgaat
     1801 tggcttatat tattagaaag ccttttattt caaatgcaac acatgacgta caggaagaat
     1861 cttcaagaag agctattaca gatatttata atgcagtaat tgaccctact cgcttgagtg
     1921 ttttagaagt tgaaaatgat aacgagaaat tttatacaac aacagttgtt gttgatgatt
     1981 ttccgttaga tatggaatat acacatttat ttgagaaagc tcaaaatatg ccttttccgg
     2041 ttgaatgcca tgtaaaagca aaaatcatta ataatgagaa agttaagaaa aaatttgacc
     2101 gtgcgaaaat aaatcataaa caacaagcaa aagaaaagca agatactggc gacagtgctt
     2161 cggaagatgt tcaagataat ttgtttgtat tgaatcaaat ggaacgagaa gtgactggct
     2221 caggtgtatt tattgagtgg gtatttatgt ttgtaattaa aagtgatgat tatagagatt
     2281 gtaaacgtaa agcaaagcgt ttgatgaaac gattaaagga aactcaaata tatgcagtta
     2341 atccattagc tgaccagtta caattgtttt atcaatgttt acatggtaat gatttattta
     2401 ttaataagta ttggttacaa agaacaacgg caacaggtat agcagagaat ttatttggtg
     2461 tgtctcaaag tttgggaaca aaaacgggtt tttatatagg gcgtattgat aaatttatac
     2521 gttctgtttc acgtgaggaa gctgttgcaa gttcaaggga tataatttta tttagtttat
     2581 tgttggcagc aaaaggtatt aagggggctg tcagtgatag tccgcatgta ttaattactg
     2641 gtcaaactgg taaaggtaaa tcatttttag caaaactatt gtggatttat acatcattct
     2701 ttaaaggtca aatgttgtac tgggatccaa aatctgagtt tgcagaatgg tttgatagag
     2761 taacagaaag tgaagaaatg caacaaaagt atcccctatt tattaatcat ttaaaaacat
     2821 ttaaatatgt aacattgaat tatgaagatt caaccaatta tggttgttta gaccctattg
     2881 tatttttaga tggtattgaa gccaatgaaa tgttgaaatc ggtgtttgat gaaattaaat
     2941 cttttgaaaa ttatcatcat attgaaacgg caatttatca agctatttct gatacggttg
     3001 aagaacgcga aaatggcaaa aaagttggtt cattaaatgt aattgaaaaa ttacagaata
     3061 atgaagatga gcatgtaaaa aatgcaggcg atttattatt tgaaaaaacg caaaacaata
     3121 ttttaaaact tgtttttagt gatggtacga atccagcttt aaatattcaa gaaaaagcaa
     3181 ctattttaca ggttaaaggt ttagatatgc ctaaagcaga tgatgataca tcaagttatt
     3241 caacaagtga gaaaaatggg attacattaa tgttattaat tggtaagttt ttagagaaat
     3301 tcggttctcg tagagatgtt caaacgacta tatttattga tgagggttgg gcttttagtg
     3361 cttcaagaca aggtaaaaaa gttggtaagc gtataaaaag aacgggacgt tctgagaata
     3421 attcacttgt atttattaca caatcggtaa aagataaagc agatgatgat ggtggtaact
     3481 ttggttgtca ttttgcattt gatgaaaaag atgaacgtga ggatatttta aaatctttag
     3541 gcttagaata ttcaaaagaa tctcctgaaa atatggagat gttgaaagat ttaaagaaag
     3601 gtcaatgtat attcagtgat ttttatggac gtgttggaaa aatggttgta cattgtccat
     3661 ttgaggaaat gactgaagca tttagaacac aagaagattc tgcaagttct aaagcagaag
     3721 aaaaatttgc gatgtaaaaa attaaaaaga tacactttat t
//
LOCUS       DAHHOJ010000161         3733 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.164, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000161 DAHHOJ010000000
VERSION     DAHHOJ010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3733
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.164"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(210..359)
                     /gene="rpmG"
                     /locus_tag="NBH32_002509"
     CDS             complement(210..359)
                     /gene="rpmG"
                     /locus_tag="NBH32_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCD1516077.1"
                     /translation="MRVNVTLACTECGDRNYITTKNKRNNPERIEMKKYCPRLNKYTL
                     HRETK"
     gene            complement(450..1967)
                     /locus_tag="NBH32_002510"
     CDS             complement(450..1967)
                     /locus_tag="NBH32_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000082539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HCD1516078.1"
                     /translation="MSQQDKKLTGVFGHPVSDRENSMTAGPRGPLLMQDIYFLEQMSQ
                     FDREVIPERRMHAKGSGAFGTFTVTKDITKYTNAKIFSEIGKQTEMFARFSTVAGERG
                     AADAERDIRGFALKFYTEEGNWDLVGNNTPVFFFRDPKLFVSLNRAVKRDPRTNMRDA
                     QNNWDFWTGLPEALHQVTILMSDRGIPKDLRHMHGFGSHTYSMYNDSGERVWVKFHFR
                     TQQGIENLTDEEAAEIIATDRDSSQRDLFEAIEKGDYPKWTMYIQVMTEEQAKNHKDN
                     PFDLTKVWYHDEYPLIEVGEFELNRNPDNYFMDVEQAAFAPTNIIPGLDFSPDKMLQG
                     RLFSYGDAQRYRLGVNHWQIPVNQPKGVGIENICPFSRDGQMRVVDNNQGGGTHYYPN
                     NHGKFDSQPEYKKPPFPTDGYGYEYNQRQDDDNYFEQPGKLFRLQSEDAKERIFTNTA
                     NAMEGVTDDVKRRHIRHCYKADPEYGKGVAKALGIDINSIDLETENDETYENFEK"
     gene            2171..3625
                     /locus_tag="NBH32_002511"
     CDS             2171..3625
                     /locus_tag="NBH32_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1516079.1"
                     /translation="MEDNKMNRSLNSRHISMIAIGGAIGTGLFVATGNIISQAGPGGA
                     ILAYLVIGVMLYFLMSSIGELATFYPVSGSFSSYSTRFIDSSLGFTMGWLYWALWSLV
                     TSVDVIVASNVLYFWDTFKFFHPITWSLIFITILLLLNIFSVKSFGETEFWLSLIKVL
                     TIIVFVIFGFLMIFGILGGHAYGFENYTKGQAPFVGGISGFLGVLLVAGFSVGGTEVV
                     AVTAGESDDPKKSMPKAIKQVFWRILLFYVLSIAVIGAIIPYTDPSLLRASSSISQSP
                     FTIVFDRVGIAFAASVINAVILTSLLSAANSGVYTTGRMLYSLSSDKKAPQFLSKLNK
                     TTKLPLRALLTTYAVVVIVIIYANFNSNAVFNLLEIIGSMIIVVWGSSIWSQIRLRQA
                     IKKQGQDPNKVLPYKAPFYPLGPIIVITTLLFLLFGGSVEYILKDQWLNAFKNFLPLI
                     ILVSIYFIHKIIHKTKFVKLETINLKPHDYDNQK"
BASE COUNT         1148 a          671 c          573 g         1341 t
ORIGIN      
        1 aaagtgactt tataaaatca gcaatctatt tccttctata aaaattctca aattttaata
       61 aagtaacaac taatacaatc atatattgtt ctaccgatga aactcaattc attaatcact
      121 ctttacaaaa atttcataaa aaagcgagct ccccaatata ataggtaagc ccgctttatt
      181 ttcaaatcgt cgtatttgaa tgataagaat tacttagttt cacgatgtaa cgtatatttg
      241 tttaatcttg ggcaatattt tttcatttca atacgctcag gattattacg tttattttta
      301 gtagtgatat agttacgatc gccacattct gtgcatgcta atgttacgtt tacgcgcacg
      361 ttaaacaccc tctcttgtta ggaatcttta cgatttatta tgatatcata aactgctcaa
      421 ctacgcaata tagaaactac atatcaaatt tatttttcaa agttttcgta tgtttcatca
      481 ttttcagttt caagatcaat agaatttata tcaataccta atgcttttgc aacaccttta
      541 ccatattctg ggtcagcttt gtaacaatga cgaatatgac gtcgtttaac atcatccgtt
      601 acgccttcca ttgcatttgc tgtatttgta aaaattcttt ctttagcgtc ctctgattgt
      661 aatctaaaca atttacctgg ttgttcaaaa taattatcat catcttgacg ttgattatat
      721 tcatagccgt atccatcagt tgggaatggt ggctttttat attcaggttg agaatcaaat
      781 ttaccatggt tatttggata ataatgtgtt cctccacctt ggttattgtc aactacacgc
      841 atttgaccat ctctactaaa aggacaaata ttttcaatac caacaccttt aggttggttt
      901 acaggaatct gccaatgatt aactcctaat cgatatcttt gcgcatcgcc atatgagaat
      961 aaacgccctt gcaacatttt gtctggagaa aaatctaatc ctggaataat attagttggt
     1021 gcaaacgcag cttgttcaac atccataaag taattatctg gatttctatt taattcaaac
     1081 tctccaactt caattagagg atactcatcg tgataccata cttttgttaa atcaaatgga
     1141 ttatctttat ggtttttagc ttgttcctca gtcattactt gaatatacat tgtccatttt
     1201 ggataatcac ctttttcaat ggcttcgaat aaatcgcgtt gagatgaatc acgatctgtt
     1261 gctataattt cagcagcttc ttcatcagtt aagttttcaa taccttgttg cgttctaaaa
     1321 tggaatttaa cccaaacacg ttcaccagaa tcattataca tagagtatgt gtgtgaaccg
     1381 aacccatgca tgtgacgtaa atctttagga atccctctat ctgacattaa gatcgttact
     1441 tggtgcaatg cttctggaag ccccgtccag aaatcccagt tattttgtgc atctctcata
     1501 tttgttctag gatctcgttt caccgcgcga tttaaactaa caaatagctt tggatctcta
     1561 aagaagaata ctggtgtgtt attccctact aaatcccagt ttccttcttc tgtgtagaac
     1621 tttaacgcaa atcctcgaat gtcacgctcc gcatcagcag caccacgttc tcctgctaca
     1681 gtagagaaac gggcaaacat ttcggtttgc ttacctattt cagagaatat tttagcattc
     1741 gtatattttg ttatatcttt agttacagta aatgtcccaa atgcaccaga acctttggca
     1801 tgcattcgac gttctggtat tacttctcta tcaaattggg acatttgctc taaaaagtaa
     1861 atatcttgca ttaaaagagg tcccctaggc cctgctgtca tactattttc tcgatctgat
     1921 actggatgcc caaaaacacc agttaacttt ttgtcttgtt gtgacatagt catccctcca
     1981 caatttataa taattataat ttaatacata ttataattta accccatcaa aattacaatt
     2041 aaacatgctc aaaatgatta aaaaatcaga aaattttaat tttttcgctt tacagcacta
     2101 aatagttatt gaatagtaca cttaaaaagc ttacaataat gggtatattt aaatagatgg
     2161 gagactaaat atggaagata ataaaatgaa ccgtagtctt aactcaagac acatttccat
     2221 gattgctata ggcggtgcaa ttgggactgg tctatttgta gctactggta atatcatttc
     2281 tcaagctggt cctggaggcg ctatactcgc ttatcttgtt attggtgtca tgctatattt
     2341 cttaatgtca tcaattggag agttggcaac attttatcca gtatcaggtt cattcagctc
     2401 ttattcaaca cgctttattg actcatctct aggctttacc atgggatggt tgtattgggc
     2461 attgtggtca ttagttacaa gtgttgatgt catagtagcg tcaaatgtgc tttatttctg
     2521 ggacacattt aaatttttcc accccattac ttggagctta atctttatta caattttact
     2581 attattaaac attttttctg taaaatcatt tggagaaact gagttttggt tatcattgat
     2641 taaagtgtta acaattatcg tattcgttat ttttggcttt ttaatgattt tcggtatctt
     2701 aggtggtcat gcatatggat ttgaaaacta tacaaaaggc caagcaccgt ttgttggtgg
     2761 tatctctggt ttcttaggcg tattattagt cgccggattt tcggttggtg gtacagaagt
     2821 agttgcagta actgctggtg aatcagatga ccctaaaaag tctatgccta aggcaattaa
     2881 acaagtattt tggcgtattc ttttattcta tgtcttatca attgcagtaa ttggtgcaat
     2941 tattccgtac acagatccat cattattaag agcaagtagt tcaataagtc aaagcccatt
     3001 tacaattgta ttcgatagag taggcatagc ctttgcagca tcagtaatca acgcggttat
     3061 tttaacttca ttattatccg ctgcaaattc aggtgtttat acaacaggaa gaatgttgta
     3121 ttccttaagt tcagacaaaa aagcacccca atttttaagt aaattaaaca agacaacaaa
     3181 gttaccttta agagcattat taactactta tgcagtcgtt gttattgtta ttatttatgc
     3241 aaactttaat tcaaatgccg tttttaattt acttgaaatt attggttcaa tgattatagt
     3301 tgtttgggga tcaagcattt ggtcacaaat acgattgcga caagctatta aaaaacaagg
     3361 tcaagatcct aataaggtcc taccatataa agcacctttt tatccattag gaccaatcat
     3421 tgtcatcact acgctattat tcttgctatt tggtggctca gttgaatata ttttaaaaga
     3481 tcaatggtta aatgctttta aaaacttttt acctttaatc attctagtgt cgatttactt
     3541 tattcataaa atcattcaca aaacaaaatt tgtaaagcta gaaacaatta atttaaaacc
     3601 acacgattat gacaatcaaa aataaataaa ttctcacacg gcagatgctt tagattatct
     3661 atagcatttg ccgcttttta atggtgtaaa gcaagatata ctttaatatc tttttctagt
     3721 ataaagtgta tta
//
LOCUS       DAHHOJ010000162         3710 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.165, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000162 DAHHOJ010000000
VERSION     DAHHOJ010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3710
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.165"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(338..525)
                     /locus_tag="NBH32_002512"
                     /pseudo
     CDS             complement(338..525)
                     /locus_tag="NBH32_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500662.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
     gene            complement(635..1237)
                     /locus_tag="NBH32_002513"
     CDS             complement(635..1237)
                     /locus_tag="NBH32_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1516080.1"
                     /translation="MNKISKALTWFIISFIIFHLILFIMWGEHQEYWYLYTGIMLIAG
                     ISYVFYQRDIESKRLLTSIGVGIITAIILIMLQLLFSLITSNLSYSSLIKELARTGVN
                     WKWQMLVTLLFVIPCHELYMRTVLQKELTHFSIPKWVAILITAICSSSLFIYLDNWWI
                     VTFIFVAQVILSLSYEYTRRIATTSVAQIVAIILLLIFNA"
     gene            complement(1252..1428)
                     /locus_tag="NBH32_002514"
     CDS             complement(1252..1428)
                     /locus_tag="NBH32_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkvS family protein"
                     /protein_id="HCD1516081.1"
                     /translation="MTVAEVGNIVEFMDGLRGRVEKINDNSVIVDLTIMENFNDLDLP
                     EKTVINHKRYKIVE"
     gene            1627..2613
                     /locus_tag="NBH32_002515"
     CDS             1627..2613
                     /locus_tag="NBH32_002515"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HCD1516082.1"
                     /translation="MKFISNNNITDPTLNLAMEEYVLKNLPAEESYFLFYINRPSIIV
                     GKNQNTIEEVNQTYIDAHNIDVVRRISGGGAVYHDTGNLNFSFITDDDGNSFHNFQKF
                     TEPIVQALQSLGVNAELTGRNDIQVGQAKISGNAMVKVKNRMFSHGTLMLNSDLDEVQ
                     NALKVNPAKIKSKGIKSVRKRVANIQEFLNDPLEIEEFKKIILKTIFGETEVEEYKLT
                     DEDWENIEKLSNDKYRTWEWNYGRNPKYNFEREEKFEKGFVQIKFDVKRGKIEHAKIF
                     GDFFGVGDVTDLENALVGCLHDFEHIEEALSEYDLYHYFGDIVRHELIRLMS"
     gene            complement(2694..2912)
                     /locus_tag="NBH32_002516"
     CDS             complement(2694..2912)
                     /locus_tag="NBH32_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IDEAL domain-containing protein"
                     /protein_id="HCD1516083.1"
                     /translation="MKYNTNVKHTTLEAFVTTVNDLGIELIINEALREVRKRQLIELI
                     DDALVNKDEAAFNQYTAEYKNLEAFLGE"
     gene            3122..3691
                     /locus_tag="NBH32_002517"
     CDS             3122..3691
                     /locus_tag="NBH32_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD1516084.1"
                     /translation="MYSQNIYVIRKGDMVIRPAFDDDDQRNGSEIIRFDKTRIQNPFK
                     VQKIIERSCKFYGNTYLGKKAETNRITGISSKPPILLTPLFPTYFFPTHSDRQNENIW
                     LNMHYIESIKELKNRKCKVTFINNESIILHVSYHSLWHQYNNSIFYYYMVDKQSRMIS
                     KNPDQPIDYNKATLNVFEALTRYSLFEDK"
BASE COUNT         1401 a          554 c          521 g         1234 t
ORIGIN      
        1 ctactatacc aatgaaagtt ttattaatat atgaacttca ttacatattc aatagaaatc
       61 acttatttat aagtactacc atataatcat aattgatttc taccacagta tgattttcct
      121 ttcaaatctg acatgtttag caggcaatat acgaaaaata cttcaaaatt ttgttacaca
      181 tctaataaac atagacaatt aaataaaaga tatgttacta taaaaatgta aaaagacgac
      241 atgcagtaac atgtcgccta atgagcccgt taaaaagacg gtgactaaat gagattttct
      301 ttaaccatca ttctatgtca aagttttgaa atgatggtta ttttttattg cttaaattta
      361 ttattgctac tactatacca atgaaagtga cgataaacat accgaaactt aacattaaat
      421 gtaatgcgtt tgcaatagat gtcacaatag gcttttcctt tcaaaagtgt taagtaatgc
      481 cataggcacc accccccttt actcagatag ccaccatcaa ttcaacttgc tcgaaaaata
      541 ttaccactat aatgaagcat gcctcgaatc ttcgtattgc tattcttaag caaaaaagct
      601 tttcttagaa cacagacgtc taagaaaagc ttatttaagc attaaaaatt aataacaata
      661 taatagctac aatttgcgca acggatgttg ttgctatgcg tcgagtatac tcataactta
      721 atgataatat cacttgtgca acaaatataa atgttactat ccaccaatta tctaaataaa
      781 taaataatga actagaacaa attgcagtta ttaatatagc tacccatttc ggtattgaaa
      841 aatgtgttaa ttctttttgt aaaacagttc tcatatataa ctcgtgacat ggaatgacaa
      901 aaagtaaagt tactaacatt tgccacttcc aattgacacc tgttcttgct aattctttaa
      961 ttaatgaact ataacttaaa ttagaagtta taagtgagaa taaaagttga agcataatta
     1021 aaataattgc cgtaataata ccaacaccaa ttgatgtaag caaccgctta gattcaatat
     1081 ctctttgata aaatacataa ctgataccag caattagcat tatacctgta tataaatacc
     1141 agtattcttg gtgttcgccc cacataataa ataatatgag atgaaatata atgaaactta
     1201 taataaacca agttaaagcc ttcgagattt tattcattat aacttacctt cttattcaac
     1261 aatcttatat cgtttatgat tgataacagt tttttccggt aaatcaaggt cattaaaatt
     1321 ttccataatt gttaagtcaa caataacaga gttatcgttg attttttcaa cacgacctct
     1381 taatccatcc ataaactcaa caatgttacc cacttctgca acagtcattt taagtcctcc
     1441 ttaaatcata actaaattat attgtactaa aattcactta acaaataaac tattaacaat
     1501 caaaaataat aaaatgtaac tttaagccga tatttagaat gatttgtgag tatataattg
     1561 gcattttgag caaagttaag gcataaataa gtagagaata tatatagata taagggagag
     1621 attgtaatga aattcattag taataataat attacagatc caactttaaa cttagcaatg
     1681 gaagaatatg ttttaaaaaa tttaccagca gaagaaagtt actttttatt ttacataaat
     1741 agaccatcta tcattgttgg aaagaatcaa aatacgatag aggaagtaaa tcagacatat
     1801 atagatgctc acaacatcga tgtagttaga agaatttctg gtggtggcgc tgtttatcat
     1861 gatactggca atttaaactt tagttttata acagatgatg atggcaacag tttccacaat
     1921 ttccaaaaat tcactgaacc aattgttcaa gcattacaat ctttaggcgt aaatgctgaa
     1981 ttaactggtc gtaacgatat tcaagttgga caagcgaaaa tctcaggaaa tgccatggtt
     2041 aaagttaaaa atagaatgtt tagtcatggt acgttgatgt taaatagtga tttagacgaa
     2101 gttcagaatg cactaaaagt taatccagct aagattaaat caaaaggtat taaatctgtt
     2161 cgtaaacgag tagcaaacat tcaagaattt ttaaatgacc ctttagaaat tgaagaattt
     2221 aaaaaaatca tcttgaaaac tatctttgga gaaactgaag tagaagaata taaattaacg
     2281 gatgaagatt gggaaaatat tgaaaagtta agtaacgaca aatatagaac ttgggaatgg
     2341 aattatggta gaaaccctaa atataacttt gaacgcgagg aaaaatttga aaaaggattt
     2401 gtacaaatta agtttgatgt taaacgaggt aaaatcgaac atgcaaaaat tttcggtgat
     2461 ttctttggtg tcggagatgt cactgatctt gaaaatgcat tagttggctg tctacacgac
     2521 tttgagcata tagaagaagc actatcagaa tatgatttat atcactattt cggtgacata
     2581 gttagacatg aattaattag attaatgtct taaacgatta aaggactatt cttacatgta
     2641 tgagatgtta agaatagtcc ttttatgatt gcgttatgga attcgaatca atgttattca
     2701 ccgagaaatg cctccaaatt tttgtattct gccgtatatt gattaaatgc tgcttcatct
     2761 ttattgacga gtgcgtcatc tataagttct atgagctgtc gttttcttac ctctcgaagt
     2821 gcttcattga taattaattc aatacccaaa tcattgacgg ttgtgacaaa cgcttctaaa
     2881 gttgtatgtt taacattagt attgtatttc atagaaacca ctacctttct ttaatttata
     2941 ataagtatac atttttctaa ttttgaaatc aatgaatatt ctgactatta gttaaaaatt
     3001 attgtacatt ttcaccaaaa taataacttg acaactcaga attttattat aaaatatctt
     3061 tatatcatta attgaatttt tatattaatg gtttaaaact taataaagcg aggttttgct
     3121 tatgtattct caaaatattt atgtgatacg caaaggagac atggttattc gaccagcatt
     3181 tgatgatgac gatcaaagaa acggtagtga aataattcgg tttgacaaaa cgcgtattca
     3241 aaatcctttt aaagtccaga aaatcattga acgctcttgc aaattttatg gtaatactta
     3301 tcttggcaag aaagcagaga caaaccgcat tactggcatt tctagtaaac cacctatttt
     3361 actaacacca ttatttccaa cttatttttt cccaacacat tctgacagac aaaatgaaaa
     3421 tatttggtta aatatgcatt atatcgaaag tattaaagaa ttaaaaaatc gtaaatgtaa
     3481 agtgacattt attaataatg aatcaatcat tcttcatgtt tcataccaca gtttatggca
     3541 tcaatataac aattccattt tttactatta catggtagat aaacaatctc gcatgatatc
     3601 aaaaaatccc gaccaaccaa tagattataa taaagccaca ttgaatgtgt ttgaagcatt
     3661 gacacgctat tctttatttg aagataaata aattgtttat ttttaaaata
//
LOCUS       DAHHOJ010000163         3651 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.166, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000163 DAHHOJ010000000
VERSION     DAHHOJ010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3651
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.166"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            41..1804
                     /locus_tag="NBH32_002518"
     CDS             41..1804
                     /locus_tag="NBH32_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1516085.1"
                     /translation="MNNQTNWIKILSGFASDSKWKIMLSILLSIISVFSGLVPYWAVF
                     KIILMMINNAYTINSIMVYIFIALIAYISQVCCFGASTMLSHITAYEILSEIRKKLAQ
                     KLMRLPLGIVESKKIGELKNIFVDKVETIELPLAHMIPEVIGNLLLSATIFLYIMLID
                     WRMASALLVTIPISIFAFKKVMSGFNETYAEQMKSNNYMNSAIVEFIEGIEVIKTFNQ
                     SQSSYKKYKDAVDNYKIHTLNWFKNTWGYMNLGASVLPSTFLGILPVGMYLISINQLN
                     YAEFFLCIVLSLGVVAPIKNFTNYVNHLKSIQYALTEVNQILSLEELVLSTKFKKPQH
                     YEIAFNNVGFSYNKDKDDLVFKHLSFTVPENNFTAIVGASGSGKSTIAKLISRYWDVT
                     SGEITIGGINIKDIESKQLNDLVGFVGQDNFLLNLTFKENIKLGNPEATDEAVEKAAK
                     LAQCHEFIKKLPDGYDTNVGTVGDELSGGEKQRVTIARMILKDAPIIVLDEATAYVDP
                     DNEQKIQEALNVLTQDKTLIVIAHRLSTIQHADQIIVLGKQQILEKGSHHLLLKLNGN
                     YKKMWDTHMHTKDWGINTGHN"
     gene            1829..3562
                     /locus_tag="NBH32_002519"
     CDS             1829..3562
                     /locus_tag="NBH32_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1516086.1"
                     /translation="MFQITFKILNWIRPYKARMILGFSMSFLNAIFIALPIFLAAKIF
                     NNVLSHKPIYMKDILNVVIIMVLLVIGRFITAYFKSKSHESIAYEMSAKERLDIGDKL
                     KNVRLGYFNSHHSNELTTIVTTDLTFLENFAMKMVDVVVNGYILITVLILSLLVVSWQ
                     VSLLACIGVLLSFLAIQLLERKSRQNAPAYHNVQNQLVEKVLEVIRGIQVIKSFAKEN
                     TSLKSFNQAVNESKRINTKIEMQYIPFNLLHLLSLKVVSIMIVLVACLLYMNHSIDLP
                     TLIMISIFSFVIFDSVENINSAAHVLEMIDMTIDDIEKIKNAPELDENGKNLTIKNEN
                     IAFQNVNFSYDDKQVIKNVNFEVPTQTSTAIIGPSGSGKSTLCHLLLRFYDIDDGNIR
                     IDGVDIKDMTLSTLMSKISAVFQKVYLFNDTIENNILFGNPGATKEEIIRVAKQACCH
                     DFIMSLPEGYQTMLNEKGSNLSGGEKQRISIARAILKDAPIIILDEATASIDPENEQL
                     IQTAINELSKGKTVITIAHKLETIKNADQIIVLNDGEIIQRGSHNELIQKPGMYQDFI
                     RIKSKSAGWKL"
BASE COUNT         1359 a          465 c          651 g         1176 t
ORIGIN      
        1 gcttaattta gcttataaat tgaaaggagt tacatatacg atgaacaatc agacaaattg
       61 gataaagata ctcagcggat ttgcttctga tagtaaatgg aaaattatgt tatcaatttt
      121 gttgtctatc atcagtgtct tttctggatt agttccatat tgggcagtct ttaaaattat
      181 tttaatgatg attaacaatg catatacgat taattcgatt atggtttata tctttattgc
      241 tttaatcgct tatatttcac aagtgtgttg ttttggagcg tcaacgatgc tatcacatat
      301 tacggcatat gaaattttat ctgaaattcg taaaaaatta gctcaaaaat taatgcgtct
      361 ccctttaggc atagtggaat ctaagaaaat aggtgaatta aaaaatatat ttgtcgataa
      421 ggttgaaaca atagaattac ctttagcaca tatgattcct gaagttatag gaaacttact
      481 tttgtcagct actatctttt tatacataat gctcattgat tggcgtatgg ctagtgcctt
      541 attagtaacg ataccaattt caattttcgc ttttaaaaaa gttatgtctg gatttaatga
      601 gacatatgct gaacaaatga aatcgaataa ttatatgaat agcgcgattg tagagtttat
      661 cgaaggcatt gaagtgatta aaacatttaa tcaatctcaa agttcatata aaaaatataa
      721 agatgcagta gataattata agattcacac tttgaattgg tttaaaaata catgggggta
      781 tatgaattta ggagctagtg tattaccttc aacattttta gggattttac cggtcggcat
      841 gtatttgata tctatcaacc aattaaacta tgcggaattt ttcctatgca tcgtattatc
      901 tttaggtgta gtagcaccga ttaagaattt tactaattat gtaaatcatt taaagtctat
      961 acaatacgca ttaactgaag tgaatcaaat attaagtcta gaagagttag tattgtcaac
     1021 aaaatttaag aagcctcaac attatgaaat tgcttttaat aatgttggat tttcatataa
     1081 caaagataag gacgatctgg tctttaagca tttatcattt acagtaccag aaaataattt
     1141 cacagcaatc gtcggggcat ctggtagcgg taagtcaacc attgccaagc ttatatcacg
     1201 atactgggat gtgacttcag gtgaaattac gattggcggt ataaatataa aagatattga
     1261 atcgaaacaa cttaacgatt tagttggttt tgttggacaa gataactttt tattaaatct
     1321 tacttttaaa gaaaatatta aacttggcaa tccagaagct acggatgagg cagttgaaaa
     1381 agctgccaag ttagcacaat gtcatgaatt tatcaaaaag ttaccagatg gatatgatac
     1441 gaatgttggt acagtgggag atgaattgtc tggtggtgaa aaacaacgag tcactattgc
     1501 aagaatgata ttaaaagatg cgcctatcat tgtattagat gaagcgactg cttacgttga
     1561 tccagacaat gaacaaaaaa ttcaagaagc attaaatgtg ttgacgcagg ataaaacatt
     1621 gattgttatt gcacatcggt tatctacgat tcaacatgca gatcaaatta ttgttttagg
     1681 taaacaacaa attttagaaa aagggtcaca tcacttattg ctaaaattaa acggtaatta
     1741 taagaaaatg tgggatacgc acatgcatac aaaagattgg ggaatcaata ctggacataa
     1801 ttaaactaat gagaaaagga ggtcaaatat gtttcaaatt acatttaaaa ttttaaactg
     1861 gataagacca tataaagcaa gaatgatttt aggatttagc atgtcatttt taaatgctat
     1921 ttttattgcg ttacctatct ttttagctgc aaaaatattt aataatgtgc tatctcataa
     1981 acctatttat atgaaagata tattgaatgt tgtaattatc atggttttat tagtgattgg
     2041 acgatttatt acagcatatt tcaaaagcaa aagccatgag agcatcgctt atgaaatgag
     2101 tgcgaaagaa cgtttagata taggggataa attgaaaaat gtaaggttag gttattttaa
     2161 ttcgcatcat tcaaatgagt taacaacaat agtaacaaca gatttaacct ttttagaaaa
     2221 ctttgctatg aaaatggtgg acgttgttgt taatggatac atattaatta cagtactcat
     2281 attgtctcta cttgtagttt cgtggcaagt atcattatta gcatgcattg gcgtattact
     2341 atcattttta gctattcaat tattagaaag aaagagtcga caaaatgcgc cagcgtatca
     2401 taatgtacaa aaccaattag tggaaaaggt attggaggtt attcgtggta ttcaagtaat
     2461 aaaatcattc gcgaaagaaa atacgagtct taaaagtttt aaccaagcag tcaatgaaag
     2521 taaacgtata aatacaaaaa tagaaatgca atatatccca ttcaatttat tgcatttact
     2581 tagtttaaaa gttgtttcaa taatgattgt attagttgca tgcttattat atatgaatca
     2641 tagtattgat ttacctaccc ttattatgat ttcaattttt tcatttgtga tatttgatag
     2701 tgttgaaaac attaatagtg cagcacacgt actcgaaatg atagatatga cgatagatga
     2761 tattgaaaag ataaaaaatg ctccagaact ggatgagaat ggaaaaaatt tgacgattaa
     2821 aaatgaaaat atcgcttttc aaaacgtgaa tttttcatat gatgataaac aagtgataaa
     2881 gaatgtgaat tttgaggtac ctacacaaac atcaacagca ataattggac cttcaggaag
     2941 tgggaaatct acattatgtc acttactctt gcgcttttat gatatcgatg atggaaatat
     3001 tcgcatcgat ggtgttgata ttaaagatat gacattaagt acgttaatgt cgaaaattag
     3061 tgcagtattt caaaaggtgt atttatttaa tgatacgatt gaaaataaca tattgtttgg
     3121 caatccaggt gcaacgaaag aagaaattat tcgtgtagcg aagcaagcat gttgccacga
     3181 cttcatcatg tcattacctg agggatatca aacaatgcta aatgaaaaag gtagtaattt
     3241 atctggcgga gaaaagcaaa ggatttctat tgctagggcg atattaaaag atgcaccaat
     3301 aattatttta gatgaagcaa ctgcaagtat tgaccctgaa aatgaacagc tgattcaaac
     3361 ggcaattaat gaattaagta aaggcaaaac agtaattaca attgcacata aacttgaaac
     3421 tattaaaaat gcagatcaga ttatagtgct caatgatggt gaaataatac aaagaggaag
     3481 tcataatgaa ttaattcaaa aaccagggat gtatcaagat tttataagaa taaaaagtaa
     3541 gtcagcaggc tggaaattat aaataatagt ttaatatgga atcagaatgt ggaaataaaa
     3601 agggtgatag ataatcaaca gaaaaataaa caggctggga tataaatccc t
//
LOCUS       DAHHOJ010000164         3606 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.167, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000164 DAHHOJ010000000
VERSION     DAHHOJ010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.167"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            11..634
                     /gene="lexA"
                     /locus_tag="NBH32_002520"
     CDS             11..634
                     /gene="lexA"
                     /locus_tag="NBH32_002520"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HCD1516087.1"
                     /translation="MRELTKRQSEIYNYIKQVVQMKGYPPSVREIGEAVGLASSSTVH
                     GHLSRLEEKGYIRRDPTKPRAIEIVSDQTSDNINMEETIHVPVIGKVTAGVPITAVEN
                     IEEYFPLPEHLTSTHNSDIFILNVVGDSMIEAGILDGDKVIVRSQTIAENGDIIVAMT
                     EEDEATVKRFYKEKNRYRLQPENSTMEPIYLDNVAVIGKVIGLYREM"
     gene            complement(1013..2029)
                     /locus_tag="NBH32_002521"
     CDS             complement(1013..2029)
                     /locus_tag="NBH32_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HCD1516088.1"
                     /translation="MKKFIVTVVAFLSIIIIAPITEFKPFIHLQNEVRQYIDIHINKE
                     TISAENKLDTPKKQQFAFNNIQMNMSKSDVEKTLNKPKRVTFNEYGTKWYTYYDDDYN
                     NFIMVSYIKDKVNALYTNQNIITSKSKIKYNTPKSVVIQRLGEPETEIVKGRVRYEQN
                     NKEYDVFHKNHIYTTVFYDKHRRNNVTAVLQVSDAMENRLKEQYGAPSKSLADSFELQ
                     NFDLVNAERKQHQLSTLKYSKQNSETARKHSKDMANNHYFDHTNLKGQSPFDRLKKDG
                     ITFNSAGENLAYGQVSSIYAHQGLMNSIGHRKNILNDTFKILGVGVDFNDEKQPFWTE
                     NYTG"
     gene            complement(2051..3028)
                     /gene="guaC"
                     /locus_tag="NBH32_002522"
     CDS             complement(2051..3028)
                     /gene="guaC"
                     /locus_tag="NBH32_002522"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015365036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCD1516089.1"
                     /translation="MKIFDYEDIQLIPNKCIVESRSECDTTIQFGPKKFKLPVVPANM
                     QTVMNEKLAKWFAENDYFYIMHRFDEEARIPFIKHMQNSGLFASISVGVKKAEFDFIE
                     KLAQEKLIPEYITIDIAHGHSDSVINMIKHIKNHIPDSFVIAGNVGTPKGVRELENAG
                     ADATKVGIGPGRVCITKIKTGFGTGGWQLAALNICSKAARKPLIADGGIRTHGDIAKS
                     IRFGASMVMIGSLFAAHEESPGETVELDGKQYKEYFGSASEFQKGEHKNVEGKKMFVE
                     HKGSLMDTLKEMQQDLQSSISYAGGKDLKSLRTVDYVIVRNSIFNGDRD"
     gene            complement(3185..3454)
                     /gene="rpsN"
                     /locus_tag="NBH32_002523"
     CDS             complement(3185..3454)
                     /gene="rpsN"
                     /locus_tag="NBH32_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCD1516090.1"
                     /translation="MAKKSKIAKERKREELVNKYYELRKELKAKGDYEALRKLPRDSS
                     PTRLTRRCKVTGRPRGVLRKFEMSRIAFREHAHKGQIPGVKKSSW"
BASE COUNT         1150 a          630 c          488 g         1338 t
ORIGIN      
        1 gtgcctatat atgagagaat taacaaaacg acaaagcgaa atatataact atattaaaca
       61 agttgttcaa atgaaaggtt atccgcctag tgttcgcgaa attggtgaag cagttggctt
      121 agcatccagt tcaactgttc atggtcacct ttcacgtctt gaagaaaaag gctatataag
      181 aagagatccg acgaaaccac gtgctataga aattgtaagt gatcaaacaa gtgataatat
      241 taatatggaa gaaacgattc atgtgccagt tattggtaaa gtcacagcag gtgttcctat
      301 taccgcagta gaaaatattg aagaatattt tccattacct gaacacttaa catcgacaca
      361 caatagcgac atattcatat taaacgtcgt aggcgacagt atgattgagg ctggtatatt
      421 agacggagac aaagtaattg ttcgcagtca aaccatagca gaaaatggag acattattgt
      481 tgctatgacc gaggaagatg aagcaactgt caaacgcttc tataaagaaa aaaatcgtta
      541 tcgattacaa cctgaaaata gtacaatgga gccaatttac ctagacaatg ttgctgtaat
      601 tgggaaagta attggtttgt accgcgaaat gtaatatttt aaaccgttat atattatcgt
      661 aattgttaag ccctcatttt tataaatttt ggacctcttg aaaaagttac gttttcaaga
      721 ggttttattt attctaatct aaattcagtt caaacagaaa ttgcgaattg ttagataatc
      781 tcattcttta atataaattt tgaattacag atttctgcaa aatgttacac aaattaaaac
      841 tcgcccccgt aataatttac aggagcgagc cattattatt ttttctttct tttttattaa
      901 gtatatcttt gacgcgttca actatatccg catcttttgg tttatctgtt gtcatcatgt
      961 ttatctactt ccttacagac aaacattacc ctttaatctc atgccaaaaa cattaaccag
     1021 tataattttc tgtccaaaaa ggttgttttt catcattaaa atcaacacca acacctaata
     1081 ttttaaacgt atcatttaaa atattttttc tgtgaccaat agaattcatt aatccttgat
     1141 gtgcatagat actactaact tgaccatatg ctaaattctc tccggctgag ttaaatgtaa
     1201 taccatcttt tttcaatcga tcaaatggtg attgaccttt taaatttgta tgatcaaaat
     1261 aatgattgtt ggccatatct ttactatgct tacgagcagt ttcagaattc tgtttagaat
     1321 acttcaatgt agataattga tgttgttttc tttcagcatt aactaaatca aaattttgta
     1381 gttcaaaact atctgcaagc gatttcgaag gtgctccata ttgttctttt aatctatttt
     1441 ccatagcatc acttacttgt aaaacagctg ttacattatt acgtcgatgc ttatcataaa
     1501 ataccgtcgt ataaatgtga tttttatgga aaacatcata ttctttatta ttttgttcgt
     1561 aacgcactct acctttaaca atctctgtct ctggttcgcc taatctttgt attacaaccg
     1621 atttcggtgt attgtattta atttttgatt ttgaagtaat gatattttga tttgtatata
     1681 acgcatttac tttatcttta atatagctta ccatgataaa attattgtaa tcgtcatcat
     1741 aatacgtata ccacttcgtg ccatattcat taaatgtaac tctttttggt ttattcaatg
     1801 ttttctcaac atctgacttt gacatattca tctgaatatt attaaaggca aattgttgtt
     1861 tcttaggcgt atccaattta ttttccgcag aaattgtttc tttattgatg tgaatgtcaa
     1921 tatattgtct tacttcattt tgtaaatgaa tgaatggttt aaattctgtt attggcgcaa
     1981 taataataat tgataaaaac gcaacaacag ttactatgaa ttttttaatt tacatcacct
     2041 actataaata ttaatctcta tcaccgttga aaatagagtt tctaacaata acataatcta
     2101 cagtacgtaa tgatttcaag tcttttccac cggcatatga aattgagctt tgtaaatctt
     2161 gttgcatttc ttttaaggta tccattaatg aacccttatg ttctacaaac atttttttac
     2221 cttctacatt tttatgttcg cctttttgaa attcagatgc actaccaaaa tattctttat
     2281 actgtttacc atcaagttct acagtttcac caggtgattc ttcgtgtgcc gcaaataatg
     2341 aaccaatcat gaccattgat gcaccaaatc taattgattt agcaatgtcg ccatgcgttc
     2401 ttataccacc atcggcaatc aaaggtttac gagctgcttt actacatatg tttaatgccg
     2461 ctaactgcca accaccagta ccaaaacctg ttttaatctt tgtaatacaa actctaccag
     2521 gacctatacc gactttggta gcatcagcac cagcattttc taattctcta acaccttttg
     2581 gcgtaccaac attaccagca ataacaaaac tatcaggtat atggtttttt atatgtttaa
     2641 tcatgtttat cactgaatct gagtgaccat gcgcaatatc tattgtaata tattcgggga
     2701 ttaatttttc ttgagctaac ttttcaataa aatcaaattc cgctttcttt acaccaactg
     2761 aaatagatgc aaataagcct gaattttgca tatgttttat aaaaggtatt cttgcttctt
     2821 catcaaaacg atgcatgata taaaagtaat cattttcagc aaaccatttc gctaatttct
     2881 cattcataac tgtttgcata tttgcaggaa ctacaggtag cttgaatttt ttcggaccaa
     2941 attggattgt tgtatcacat tcagacctac tttcaactat gcatttatta ggtattaatt
     3001 gaatatcttc gtaatcaaat attttcactt taaatagccc ctttaaaaac taattatttt
     3061 taaatgcaaa ttaaaaaccg aatgtttcct tattaatatg agaaaacatt cagtttatag
     3121 cattcgtata tactgtacat tgttttattg atattgtaaa tgggctaaag tacgaaattt
     3181 gattttacca acttgatttt ttaacacctg gaatttgccc tttgtgcgca tgttctctaa
     3241 acgcaatacg agacatttca aatttacgta atacacctct aggtcttcca gttactttac
     3301 atcttctagt taaacgtgta ggtgatgaat ctcttggtaa ttttcttaac gcttcgtaat
     3361 caccttttgc ttttaactct ttacgtaatt cgtaatattt atttactaac tcttctcttt
     3421 ttctctcttt tgctatttta gatttcttag ccatatatag ttacctcact ttttatcaaa
     3481 tcgtaattat tacgatttat ataataacac aatcctcaca taattcaaca gtaaagtttc
     3541 atttctttgt taaatcattt tctaaaccat catatttaag attaaagtgt attatcgaac
     3601 atttat
//
LOCUS       DAHHOJ010000165         3582 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.168, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000165 DAHHOJ010000000
VERSION     DAHHOJ010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.168"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            190..915
                     /locus_tag="NBH32_002524"
     CDS             190..915
                     /locus_tag="NBH32_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL14"
                     /protein_id="HCD1516091.1"
                     /translation="MKKNIMNKLVLSTALLLLGTTSTQLPKTPISFSSEAKAYNISEN
                     ETNINELIKYYTQPHFSLSGKWLWQKPNGSIHATLQTWVWYSHIQVFGAESWGNINQL
                     RDKYVDIFGTKDEDTVEGYWTYDETFTGGVTPAATSSDKSYKLFLKYSDKQQTIIGGL
                     EFYKGNKPVLTLKELDFRIRQTLINNKKLYNGEFNKGQIKITGGGNNYTIDLSKKLKL
                     TDTNRYVKNPRHAEIEVILEKSN"
     gene            988..1713
                     /locus_tag="NBH32_002525"
     CDS             988..1713
                     /locus_tag="NBH32_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL13"
                     /protein_id="HCD1516092.1"
                     /translation="MKKNITKKIILSSALLLLGAVSTQLPNTPISSKSEAKAYYINQE
                     ETNVSELIKYYTQQHLTFSNKWLWQKDNGTIHATLLQISWYSHIQVFGPESWGNINQL
                     RDKYVDIFGIKDQETIDSYALSQETFTGGVTPAAKPNDKHYKLNVTYKDKDETFIGGY
                     PFYTGNKPVLTLKEVDFRIRQTLIKNKKLYQDKYNKGQLTITGDDNNYTIDLSKRLPS
                     TDANKYVKNPQNAKIEVTLEKSN"
     gene            1823..2539
                     /locus_tag="NBH32_002526"
     CDS             1823..2539
                     /locus_tag="NBH32_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superantigen-like protein SSL12"
                     /protein_id="HCD1516093.1"
                     /translation="MKKNITNKIILTATLLILGTSSAQLPNTPIGFSSEAKAYHIGQD
                     ETNINELIKYYTQKPLTFSNRWLYQYDDGNIYVEFKRYSWSAHIRLWGAESWGNINQL
                     RDRYVDVFGLKDKDTRRLWWVYTDTFTGGVTPAASSSDKPYNIYVQYKDKLQTFIGAH
                     KIYQGNKPVLTLKEIDFRVRESLIKNKILYNENRNKGNIHITGGGNNFTIDLSKRLHS
                     DLANVYVKNPQKITVEVLID"
     gene            complement(2933..3157)
                     /locus_tag="NBH32_002527"
     CDS             complement(2933..3157)
                     /locus_tag="NBH32_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516094.1"
                     /translation="MLIEERGLKMKELNEIINAIQFLFESESGYKISKNSGVPYQTVQ
                     DLRNGKTKIEDARFRTIIKLYSYYATLKEQ"
     gene            complement(3114..3260)
                     /locus_tag="NBH32_002528"
     CDS             complement(3114..3260)
                     /locus_tag="NBH32_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516095.1"
                     /translation="MKLFYIAFLIIIWLNIFLGNEIIHTLTVLITTLYIVNRRKRIKN
                     ERVE"
BASE COUNT         1322 a          563 c          510 g         1187 t
ORIGIN      
        1 tttttaatat ataactctcg cctactttat taggtactgt ttctgtccaa aaattaaaaa
       61 tgataagttt tgcttaaata acactactaa ctgtttaagt ttatttaaca tagttttagc
      121 ttttatttaa ttccgaatcg gtgtaaaagc ttatatactt tgggtaattc acgcaaagga
      181 gattttcata tgaaaaagaa catcatgaat aaattagttt tatcaacagc attgttactt
      241 ttaggaacca catcaacaca acttcctaaa acgccaatca gtttttcatc tgaagcaaaa
      301 gcctataata tcagtgaaaa cgagactaat atcaatgaac taatcaaata ttacactcag
      361 ccgcattttt cattatctgg aaaatggcta tggcaaaagc ccaatggtag cattcatgca
      421 acattgcaaa cgtgggtttg gtatagtcat attcaagtat ttggtgctga aagttgggga
      481 aacattaatc agttaagaga taaatacgtt gatatattcg gaactaaaga tgaggataca
      541 gttgaaggtt actggactta tgatgaaaca tttacaggtg gtgtaacacc tgcagctaca
      601 tcatccgata agtcttataa attattttta aaatatagcg ataaacagca gactatcatc
      661 ggtggtctcg aattttacaa aggaaataaa ccagtattaa ctttaaaaga actagatttc
      721 cgtatccggc aaacattaat aaataataaa aagctatata acggagaatt taataaaggt
      781 caaattaaga ttacgggtgg cggtaataat tatacgattg atttaagtaa gaagttaaaa
      841 ttaactgaca caaaccgtta tgttaaaaat cctcgacatg cagaaattga agtcattctt
      901 gaaaaatcta attaacctta attgcggagt aaatactaaa attatttaag taacccttat
      961 ttataaaaaa tacaaggagc attttaaatg aaaaaaaaca ttacgaaaaa aattatttta
     1021 tcatcagcgt tgttactatt aggcgctgta tcaacacaac ttcctaacac accaatcagt
     1081 tctaaatctg aagctaaagc ttattatatt aatcaagagg aaacaaatgt aagtgagtta
     1141 atcaagtatt acactcagca acatttaacc ttttcaaata aatggttatg gcaaaaagat
     1201 aatggcacaa ttcatgcaac gttacttcag atatcatggt atagccatat tcaagtattt
     1261 ggccctgaaa gttggggtaa tattaatcaa ttaagagata aatatgtaga tattttcggc
     1321 ataaaagacc aagaaactat tgactcatat gcactatctc aagaaacatt tactggtggc
     1381 gttacacctg cagcaaaacc taacgataaa cactataaat tgaatgtaac atataaagat
     1441 aaagatgaaa catttattgg tggatatcca ttttatacag gtaataagcc agttttaact
     1501 ttaaaagaag ttgatttccg tatccgacaa acattaatta aaaacaaaaa attatatcaa
     1561 gataaatata ataaaggtca actcacgatt actggtgacg ataataatta cacaattgat
     1621 ttaagtaaga gattaccgtc aactgacgca aataaatatg ttaaaaatcc tcaaaatgcc
     1681 aaaattgaag ttaccctcga aaaatctaac tagtatcgaa atcggagtca acaaataata
     1741 atctcattca ttatattgac gccgctcact tttatatttg atattcctcg caatgataaa
     1801 tccacagaaa ggagatgttc aaatgaaaaa gaacatcacg aacaaaataa tattaacagc
     1861 aacattgtta atattaggta cttcttcagc tcaacttcct aacacaccaa tcggtttttc
     1921 atctgaagct aaagcttatc atattggcca agacgaaacg aacatcaatg aattaatcaa
     1981 atattacaca caaaagcctc tcacattttc aaatagatgg ttataccaat atgatgacgg
     2041 caacatttat gttgaattta agcgttattc ttggtctgca catatacgct tatggggtgc
     2101 tgaaagttgg ggcaacatta atcaattaag agaccgctat gtagacgtgt ttggtttaaa
     2161 agacaaagac actagacgat tatggtgggt atatactgat acatttacag gtggtgttac
     2221 acctgcagca tcgtcttctg ataagcctta taatatttat gtacaatata aagataaact
     2281 acaaacattt atcggcgcac ataagatata tcaaggtaac aaaccagttt taacgttaaa
     2341 agaaattgat ttccgagtac gtgaatcatt aataaaaaat aaaatactat ataatgaaaa
     2401 tcgcaataaa ggtaatattc atattaccgg tggtggtaat aactttacaa ttgatttaag
     2461 taaaagatta cattctgatc ttgcaaatgt ttatgttaaa aatcctcaaa aaataacagt
     2521 cgaagtcctc attgattaaa taaggtagta tgatttatat cgaaaatact aaggataaac
     2581 aataattgag taatactaca aaatgtatca ataatattta cacaatattt aaattagtac
     2641 acagaatatt aaatgggatt tggagtctaa tagaaatgca aaatcattct ctgtgttggg
     2701 ggccgctcca acttgcattg tctgtagaat ttctttttga aattctctgt gttggggccc
     2761 atccccaact tgcacattat tgtaagctga cttttcgtca gcttctgtgt tggggcccca
     2821 ccccataatt gaaaaatgtt tgttacatgg gcatttttgt ttggtcaata tttgtctata
     2881 taatattgta gagcctaaga cgtggttacg ttttaggctc ttaattcaat tattactgct
     2941 cttttaatgt agcataataa ctatatagtt ttataatcgt tctaaatcta gcatcttcta
     3001 ttttcgtttt accattcctc aaatcttgca cggtctgata cggcacccct gaatttttcg
     3061 atattttgta gcctgattca gactcaaaca aaaattgaat agcgtttatt atttcattca
     3121 actctttcat ttttaatcct ctttcttcta ttaacaatat acaatgttgt tattaaaaca
     3181 gtcagtgtat ggatgatttc atttcctaaa aatatattca gccatataat aattagaaat
     3241 gcaatataaa atagtttcat tataatcccc tatcattttt atatttatca ctttccattt
     3301 agtttcaaca ttaaaagcta acctaggttt aagattatct tataacgcct ataactagaa
     3361 gtctgcattt tatcttgtat ataacatttt ataatgttac actacacctt tttatatttt
     3421 tcacaagaag ttttcacgaa ttattatatt taaactcacc catattccaa cacaattaat
     3481 ttttctctat tcataattga ttgatcatac tagttaatat acagttaact tttatttaat
     3541 agttaattaa ttgatttaat tctaagatat ttgtattttt at
//
LOCUS       DAHHOJ010000166         3384 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.169, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000166 DAHHOJ010000000
VERSION     DAHHOJ010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3384)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3384)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3384
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.169"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..545
                     /locus_tag="NBH32_002529"
     CDS             <1..545
                     /locus_tag="NBH32_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000492905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1516096.1"
                     /translation="MLVIFLVINYGTDVSYDHEYVGLFIILLGVVLLKVLNINLLSFK
                     KLKFTHMLYIIFGFLLMYGLDYLYDMFAPPTLNEILLDEESEDAPYHIALLSTAIIPP
                     ITEEIICRGLIIRILFRNHLFLGFIVSTVFFTLIHESDTLIGYLPYFYSGLIFGYTYL
                     KTKRLEVPILIHFINNLLAM"
     gene            complement(942..1655)
                     /locus_tag="NBH32_002530"
     CDS             complement(942..1655)
                     /locus_tag="NBH32_002530"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCD1516097.1"
                     /translation="MAKLNVEVFADGADIEEMKAAYKNKQVDGFTTNPSLMAKAGVTD
                     YKAFAEEAVKEIPDASISFEVFADDLETMEKEAAILKQYGENVFVKIPIVNTKGESTI
                     PLIKKLSADNVRLNVTAVYTIEQVKEITEAVTEGVPTYVSVFAGRIADTGVDPLPLMK
                     EAVKVTHSKDGVKLLWASCRELFNVIQADEIGADIITCPADVVKKVNTNLGRDINELS
                     VDTVKGFAKDIQSSGLSIL"
     gene            1914..2216
                     /locus_tag="NBH32_002531"
     CDS             1914..2216
                     /locus_tag="NBH32_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516098.1"
                     /translation="MSRSKKYFYLSSLMIILSFFFNTNNVFLSGLFNSFIKLILFCSV
                     INSIILILSIIFADRSIKSLKPDADWIRIASKSLPWIILIVILVHIFSIVHTFGFI"
     gene            2472..2837
                     /gene="crcB"
                     /locus_tag="NBH32_002532"
     CDS             2472..2837
                     /gene="crcB"
                     /locus_tag="NBH32_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCD1516099.1"
                     /translation="MQYVYIFIGGALGALLRYLISFLNTDGGFPIGTLIANLTGAFVM
                     GLLTALTIAFFSNHPTLKKAITTGFLGALTTFSTFQLELIHMFDHQQFITLLLYAITS
                     YVFGILLCYVGIKLGGGLS"
     gene            2834..3187
                     /locus_tag="NBH32_002533"
     CDS             2834..3187
                     /locus_tag="NBH32_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CrcB family protein"
                     /protein_id="HCD1516100.1"
                     /translation="MISIILVMIGGGLGAIARSAVTDYFNHKFTSKLPIATLIVNLVG
                     SFLIGLTIGLSISISWFPAFFVTGFLGGLTTFSTLAKELTLMMMPKFNINLFLNYSLL
                     QFIIGFIACYIGYHI"
BASE COUNT         1064 a          562 c          470 g         1288 t
ORIGIN      
        1 aaatgttggt cattttttta gtaattaatt atggaacaga cgtcagttat gaccatgagt
       61 atgttgggct atttattata ttactaggtg ttgtacttct taaagtttta aatatcaatt
      121 tattatcttt caaaaaatta aaattcactc atatgcttta tattatattt gggtttttgt
      181 tgatgtatgg attagattac ttatatgata tgttcgcacc gcctacatta aatgaaatat
      241 tgctagatga agagtctgaa gatgcacctt accacatagc tctgcttagt actgctatca
      301 ttccaccaat tacagaagaa atcatctgtc gtggattgat tatacgaatt ttattcagaa
      361 atcatctttt cttaggtttt attgtttcaa ctgtattctt cacgctaata catgaatcag
      421 acacgctgat tggttatctt ccgtattttt attcaggttt aatttttggc tatacttact
      481 taaaaacaaa acgattagag gtaccgattt taatacattt tatcaataac ctcttagcca
      541 tgtaacagta aaatcattaa ttattttgat atttacatga tgacaccaaa gcttttacaa
      601 agtattaata ggtgcctttc caaatgttat tgcatattta cgttctgtaa ttatatcaaa
      661 acaacaaaac atattaagtt tagctatgaa aaaaatctat gacgatagat tttttcatag
      721 ctatttatta tagttattga gcctatccca gactctctat actgttatat tagcagtcat
      781 tgactgaatg ataatacgct tttcaattat aggggcccca acacagagaa tttcgaaaag
      841 aaattctaca ggcaatgaaa tacatattag tttaaaattg actccaatat gaaaaacccc
      901 cataagcaca atgcctatga gggtttaaat atatttatat attatagaat agaaagacct
      961 gaagattgaa tatctttcgc aaagccttta actgtatcta ctgataattc gttaatatcg
     1021 cgacctaagt ttgtattcac ttttttcaca acatctgcag ggcatgtaat aatatctgca
     1081 ccaatttcat cagcttgaat cacattgaat aattcgcggc aacttgccca taataattta
     1141 acgccgtctt tactatgcgt aacttttaca gcctctttca ttaatggtaa tggatctacg
     1201 cctgtatctg caatacgtcc tgcaaatact gaaacatatg ttggcacacc ttcagttact
     1261 gcttcagtta tttctttaac ttgttcaata gtgtaaacag ccgtaacgtt taatctcaca
     1321 ttgtcagctg aaagtttttt aattaaagga atcgttgatt caccttttgt atttacaatg
     1381 ggaattttaa caaatacatt ttcgccatat tgttttaaaa ttgctgcttc tttttccata
     1441 gtttctaaat cgtctgcaaa tacttcaaat gaaattgaag catctggaat ttcttttaca
     1501 gcttcttcag caaaagcttt gtaatctgtt acgcccgctt tcgccattaa actaggattt
     1561 gttgtaaaac catccacttg tttgttttta taagctgctt tcatttcttc aatatctgca
     1621 ccatccgcaa atacttctac atttagttta gccatataat atagcctcct tgattcttat
     1681 taaaatttta acaacatctg catgtctttt tcttacaacc atttgtaaaa aatgattttt
     1741 atttctttgt tatataaaac atttatatca cgcttttata atatatagct aattttttgc
     1801 ctagatttct taaaaaagac tttagcaaca ttgtgtcaac atggtaatta aactattgtt
     1861 ttatgattgt ttatgatacg atatgtaaat gataaccatc caggaggtct agcatgtctc
     1921 gttcaaaaaa atacttttac ttatctagct taatgattat tttaagcttt ttctttaata
     1981 caaataacgt tttcctaagt gggcttttta attcttttat taaattaata cttttctgca
     2041 gtgttattaa ctcaattata ctaatattgt ctataatttt tgcagatcgt tcaattaaat
     2101 cactaaagcc tgatgcggat tggattagaa ttgcgagtaa aagtttgcct tggattattc
     2161 taatcgttat tttagtacat atcttttcga ttgttcacac attcggtttt atttaaaaaa
     2221 gataaaatgt caatgtagcc ttaatacaaa acaatacaat gcatcatgct ataatgagta
     2281 aatcaatttg atgatgttgt tgcgtttaaa aaatattaga ttttcgaaat cataactatg
     2341 catctaatcg ctatagttat acaacaagat ataacatata atgaggtttg ataatgcatc
     2401 gacaattttt gtcgtcgcgt tgccaaaacc tcttttttaa attcaaacta cttcttttcg
     2461 aggtgaacca aatgcaatat gtatatattt ttatcggtgg tgctttaggc gctttattac
     2521 gttacctcat ttcttttctg aatactgacg gaggttttcc aatcggaaca ctgatagcca
     2581 atttgactgg tgcctttgta atgggattgc taacagcctt aacaattgca tttttttcaa
     2641 accatccgac cctaaaaaaa gctattacga ctggttttct tggtgcatta acgacttttt
     2701 caacatttca attagaatta atacatatgt ttgatcatca acaatttata actttactac
     2761 tatatgctat aacaagttat gtctttggta ttttgttatg ttacgtcggt ataaaactag
     2821 gtggtggttt atcatgatat caatcatttt agtcatgatt ggcggcggtt tgggtgcaat
     2881 tgctagaagt gccgttactg attattttaa tcataaattt acttccaagt tacctatcgc
     2941 aacattgata gtaaatcttg ttggtagttt tttaattgga ttaactatag gcttatcaat
     3001 ttcaatctca tggttccctg cgttctttgt taccggtttt ttaggtggct taacaacttt
     3061 ctcaacgtta gccaaggaac ttacactaat gatgatgcca aaatttaata ttaacctttt
     3121 tctcaattat tcacttttac aattcatcat tggatttata gcttgttata ttggctatca
     3181 tatttaaaaa taaaatgctt cattcagcaa ataggcaaat tacgacacct tcctgaacga
     3241 agcatttttt tattttcatg caaatttttt agcaccataa aatgcctacc aaatttcaat
     3301 aatctttgtt accgtttaaa taatgtgaat gtcaataaat tctccaaact agtcgaaaat
     3361 aaaacaggct gggacataaa tccc
//
LOCUS       DAHHOJ010000167         3373 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.170, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000167 DAHHOJ010000000
VERSION     DAHHOJ010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3373)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3373)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3373
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.170"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(331..1167)
                     /locus_tag="NBH32_002534"
     CDS             complement(331..1167)
                     /locus_tag="NBH32_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014532577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HCD1516101.1"
                     /translation="MDLASKYFNGVNWRYIDHSSGLEPMQSFAFDDTFCESVGKDISD
                     NVVRTWIHQHTVILGIHDSRLPFLKDGIDYLTNEIGYNAIVRNSGGLGVVLDQGVLNI
                     SLMFKGQTETTIDEAFTVMYLLISKMFEDENVDIDTMEIEHSYCPGKFDLSIDGKKFA
                     GISQRRVRGGIAVQIYLCVEGSGSERALMMQTFYEHALKGEVTKFKYPEIEPSCMASL
                     ETLLNKTITVQDVMFLLLYAIKDLGGVLNMTPITQEEWQRYDTYFDKMIERNKKMIDQ
                     MQ"
     gene            complement(1170..2156)
                     /gene="pta"
                     /locus_tag="NBH32_002535"
     CDS             complement(1170..2156)
                     /gene="pta"
                     /locus_tag="NBH32_002535"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCD1516102.1"
                     /translation="MADLLNVLKDKLSGKNVKIVLPEGEDERVLTAATQLQATDYVTP
                     IVLGDETKVQSLAQKLNLDISNIELINPATSEFKAELVQSFVERRKGKATEEQAQELL
                     NNVNYFGTMLVYAGKADGLVSGAAHSTGDTVRPALQIIKTKPGVSRTSGIFFMIKGDE
                     QYIFGDCAINPELDSQGLAEIAVESAKSALSFGMDPKVAMLSFSTKGSAKSDDVTKVQ
                     EAVKLAQQKAEEEKLEAIIDGEFQFDAAIVPGVAEKKAPGAKLQGDANVFVFPSLEAG
                     NIGYKIAQRLGGYDAVGPVLQGLNSPVNDLSRGCSIEDVYNLSIITAAQALQ"
     gene            2324..3076
                     /locus_tag="NBH32_002536"
     CDS             2324..3076
                     /locus_tag="NBH32_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-dependent peroxidase"
                     /protein_id="HCD1516103.1"
                     /translation="MSQAAETLDGWYSLHLFYAVDWASLRLVPKNERDALVTEFQSFL
                     ENTATVRSSKSGDQAIYNITGQKADLLLWFLRPEMKSLNHIENEFNKLRIADFLIPTY
                     SYVSVIELSNYLAGKSDEDPYENPHIKARLYPELPHSDYICFYPMNKRRNETYNWYML
                     TMEERQKLMYDHGMIGRKYAGKIKQFITGSVGFDDFEWGVTLFSDDVLQFKKIVYEMR
                     FDETTARYGEFGSFFVGHIINTNEFDQFFAVS"
BASE COUNT         1089 a          620 c          491 g         1173 t
ORIGIN      
        1 atagccctac aatttataat tttccaccct aactataata ctacaaataa taattggaat
       61 atatagattt actactaaag tattagaaca tttcaataga aggccgtttc tttcatagtc
      121 atacgcatta tatataccct attctcaatc tatttaatac gtaaaacatg aaattttctt
      181 attaaattta ttatttcaat catatcatta cttttaattt aatgatgttc aatttaaata
      241 ttaggccaat aacatattta tgctatttat ggatactttc aaaaataaca gccccaaacg
      301 ataacttgaa aggggctgtt aaatatttaa ctattgcatt tggtctatca ttttcttgtt
      361 tctttcaatc attttatcaa aatacgtatc gtatctttgc cattcttctt gagtaatggg
      421 cgtcatattt aatacaccgc caagatcttt gattgcatat aatagtaaaa acattacatc
      481 ttgaacagta atcgttttgt taagcaatgt ctctaatgag gccatacaag atggttcaat
      541 ttcaggatat ttaaatttag tcacttcacc ttttaaagca tgttcataaa atgtttgcat
      601 catcaatgca cgttctgaac cagagccttc aacacaaaga taaatttgta cagcaatacc
      661 gcctctaact cttcgttgcg atatgcctgc aaatttttta ccatcgatac ttaagtcaaa
      721 ttttcctggg caataagaat gttcaatttc catcgtatca atatcaacat tctcatcttc
      781 gaacattttg ctaattaaaa gatacatcac agtaaacgct tcatcaatcg ttgtttctgt
      841 ttgtcctttg aacatcagcg atatatttaa tacaccttga tctagaacga cacctaagcc
      901 accagaattt ctaacaatgg cattataacc aatctcattc gttaaataat caatgccatc
      961 ttttaaaaac ggcaatcttg aatcatgaat accaagaata acagtatgtt gatgaatcca
     1021 agtacgcaca acattatctg atatatcttt gcccacactt tcacaaaatg tatcatcgaa
     1081 tgcgaaagat tgcataggct ctaatccaga agaatgatcg atatatcgcc agttgacgcc
     1141 attaaaatat ttactcgcta aatccatcgt tattgtaagg cttgcgctgc tgtgataatt
     1201 gaaagattgt atacatcttc aattgaacag ccacgtgata agtcatttac tggagaattt
     1261 aaaccttgta atactggacc aactgcatca tatccaccta aacgttgtgc aattttgtaa
     1321 ccaatattac cagcttctaa acttgggaat acaaagacat ttgcatcacc ttgtaattta
     1381 gcacctggcg cttttttctc agcaacacct ggtacaatcg cagcatcaaa ttggaattct
     1441 ccatcaatga ttgcttctaa tttttcttct tcagcttttt gttgtgctaa tttgacagct
     1501 tcttgaactt ttgtcacgtc gtctgattta gcagaccctt ttgttgaaaa gcttaacatt
     1561 gcaacttttg gatccatgcc aaagcttaat gctgattttg cactttctac tgcaatttct
     1621 gcaagtcctt gtgaatcaag ttctggattg attgcacaat caccaaagat gtattgttca
     1681 tcacctttaa tcataaagaa gatacctgat gttcttgata cacctggttt cgttttgatg
     1741 atttgtaaag ctggacgcac agtgtctcct gttgaatgtg ctgcaccact aactaaacca
     1801 tctgctttac cagcataaac aagcattgta ccgaagtagt tcacattgtt taataattct
     1861 tgcgcttgtt cttcagtcgc tttaccttta cgtcgttcaa caaatgattg gactaattca
     1921 gctttaaatt cacttgtcgc aggattaatt aattcaatat tagaaatatc aagattaagt
     1981 ttttgcgcta aagattgaac cttagtctca tcacctaaca cgattggtgt aacataatct
     2041 gttgcttgta attgtgttgc agctgttaga acacgctcgt cctctccttc aggtaatacg
     2101 attttaacgt ttttaccaga aagtttgtct tttaatacat ttaataaatc agccataatg
     2161 tcctcctgta atataaatct tattaatcat tcacggtata attatacgcc attttatttc
     2221 ataatgccac gtcaccaatt tgtaatcata attttatcgt tacccctttg ctttatgata
     2281 aaatttataa agaactgatg atttttgaaa aggagcgata aacatgagtc aagcagccga
     2341 aacattagat ggttggtata gtctacattt attttatgca gttgattggg catcattacg
     2401 tctagttcca aagaacgaac gcgatgcact tgtcactgaa tttcaatcat ttttagaaaa
     2461 tacagcaact gtaagatcat caaaatctgg tgatcaagct atttataata taactggtca
     2521 aaaagcagat ttgttattat ggttcttacg tcctgaaatg aagtctttaa atcatattga
     2581 aaatgaattt aacaaattgc gcattgctga cttcctaatc cctacatatt catatgtttc
     2641 agtcattgaa ttgagcaatt atttagctgg taaatctgat gaagatcctt atgagaaccc
     2701 tcatatcaaa gcaagattat acccagaatt accacattct gattatattt gtttctatcc
     2761 aatgaacaaa cgtcgtaatg aaacttataa ctggtacatg ttaactatgg aagaacgcca
     2821 aaaattaatg tatgaccatg gtatgattgg tagaaaatat gctggcaaaa tcaaacaatt
     2881 tattactggt tctgtagggt ttgatgattt cgaatggggc gtaacattgt tctcagatga
     2941 cgtattacaa ttcaaaaaaa ttgtatacga aatgcgcttt gatgaaacaa cagcacgata
     3001 cggtgaattc ggtagtttct ttgtaggaca tattattaac acaaacgaat tcgatcaatt
     3061 ctttgcggtt tcttaataca ttggtacgtt tataaattaa taaaaaattc caagcttatc
     3121 ggtttaagct tggaattttt cgtttatctt cagtatattc ccgtatacat aagacgtgat
     3181 ttggtaaata gttgaaatct gtatgtttaa acttatatat atgtgctaat gtattatcaa
     3241 taacaaagta cactttgctc atagcaaagt gtacccgagt agtcttcctt gggagaactt
     3301 taactactat cactacatat aaacgttaac ctcaatagaa attatacagt cgctactcta
     3361 tacaattttt gta
//
LOCUS       DAHHOJ010000168         3370 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.171, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000168 DAHHOJ010000000
VERSION     DAHHOJ010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3370
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.171"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(129..455)
                     /locus_tag="NBH32_002537"
     CDS             complement(129..455)
                     /locus_tag="NBH32_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HCD1516104.1"
                     /translation="MILDNVNPNDLFPTEKKGPSVLGIIEYQVQGENEFEGAFIATNE
                     RLIMNVDMNGQFYYRSISYNEIEKIDYDGQTIMFKFNIGNVPMHDIKSDNVEMFVEYV
                     KQHMIV"
     gene            complement(519..1262)
                     /locus_tag="NBH32_002538"
     CDS             complement(519..1262)
                     /locus_tag="NBH32_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCD1516105.1"
                     /translation="MSKFKVKRLTAHILMLKVINDMLKYSFHLTLTQVKLLNLLVKYD
                     NSDSNSASIDRLLKMKEIQSKMALLQMLSYLNNHQWLLKGRDQRDQRKLTISINKMHI
                     DKIEYMNNELSDYIERYFGQYTFDFSCDYVSYLLSSQELFRNLKCYLNMCQLSLEELY
                     VLGILNLHKGQLTVKELQGEFHHPIFAVSPILKCLILKGLVKKERCELDERRVIVTIK
                     REKFSKVTMLIQACYNYLEKGIQVKLNNK"
     gene            complement(1288..3243)
                     /locus_tag="NBH32_002539"
     CDS             complement(1288..3243)
                     /locus_tag="NBH32_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1516106.1"
                     /translation="MTDNYSLHIFKSMKMSSVKEANLKIIYWLQGTGVVSINLQQYDV
                     KRNDVTVIMLNDLYQIDGHDDSVCCVVEVSAKTFLKFMNANYMMRGKILTNEVASTFR
                     ILIKYLIYSKIKQQPYNANDKIINYMCVELMSLNTYDGSRHLVAEEVHDYLTNNHHKK
                     INRKDVINQVSITNKALSDMFKATPYSNFIQYLNHIRLEYCLIDILTSKKPIEEIASS
                     HGFNHYSRFIHLFKETYGDTPKLIRKRYSPTSHSIYHSQLVEIDKNIIELFDDTNQSS
                     SSMREIDIPFEPTKRSQMYQPKNIYIKGSNFYWMDYYTIKQIIQRLSVNPENLHIIVT
                     IDTSNELSIEQITLLLQKIVNYNVNVVFRIKHEYKELLTSTERGKIEKLVATIFNMTF
                     ESNRCKIGFLINDLNTTAIQKLKRLIDNYIGVFELIYHLEPNELNVRYYKEIDHLIDY
                     FILPLRQIEQVSIARSKIICDVDLINENDDLKEKITMSDINKITELLTNYTKFRGLIM
                     HMSIISEASKEINLTRLSIFIFIINILNQLRGIVIYQNDTMIVTKFKHEVQCVICLPA
                     ALLNENSDAIRLSCKSLQQFSHAKTKQIVLSIDKDNHTSENGVLVNDIMQQHSYWKAN
                     FIERNIYDHSLMVSSNSIVHLKYHLEI"
BASE COUNT         1144 a          569 c          373 g         1284 t
ORIGIN      
        1 tgatggcttg ctcacgaaat ttataaaata taaaaaaaga agagctttaa atcataacgc
       61 atgccattta tctttaaatg caatgagtta tcattcaaac tcttcttatc aacctacata
      121 cattgacatt atactatcat atgttgtttt acatattcaa caaacatttc aacgttatca
      181 cttttaatat catgcattgg gacattacca atattaaatt tgaacattat agtttgacca
      241 tcataatcta tcttctctat ttcattataa ctaatacttc tataataaaa ttggccattc
      301 atatctacat tcataattaa tctttcattt gttgctataa atgccccttc aaattcattc
      361 tcaccttgaa cttgatactc aattattcct agcactgacg gacccttttt ttcagttgga
      421 aataaatcat ttggattcac attatctaaa atcataatat ccctcccact taaaactata
      481 aactattctt cataggtata tgaaatacaa gtgattaact atttattatt aagcttaact
      541 tgtattccct tttcaagata attataacat gcttgaataa gcatagtgac tttagaaaac
      601 ttttctctct tgattgttac aataacacgc ctttcatcta attcacatct ctctttttta
      661 actaatcctt tcaaaattaa acactttaaa atcggactaa ccgcaaatat cgggtgatgg
      721 aattcaccct gtaattcttt aaccgtcaat tgacctttat gcagatttaa tattcctaaa
      781 acgtataact cttctaatga taattgacac atatttaaat aacactttag atttcgaaat
      841 aattcttgtg acgacaacaa atacgataca tagtcacaac taaaatcaaa tgtatattga
      901 ccaaaataac gttcaatata gtcactaagc tcattattca tatattctat tttatctata
      961 tgcattttat taatcgaaat agtcaattta cgttgatccc tttgatctct tcctttcaat
     1021 agccattgat gattgtttaa ataacttaac atttgaagaa gtgccatctt agattggatt
     1081 tctttcattt ttaataacct atctattgaa gcgctattgg aatcagaatt atcatattta
     1141 actaatagat ttaatagttt cacttgagtc aaagtgagat gaaatgaata tttcaacata
     1201 tcattaatga ctttcaacat tagtatgtgt gcagttaaac gcttaacttt aaatttagac
     1261 atctttaaaa cctctcttaa accatgccta tatctcaaga tgatatttca aatgaacaat
     1321 actattgctt gagaccatta atgaatgatc ataaatattt ctttctataa aattagcttt
     1381 ccaataactg tgttgttgca taatatcatt cacaagtaca ccattttcgg aagtatgatt
     1441 atctttatct atacttaaaa caatttgttt agttttagca tggctaaatt gttgaagact
     1501 cttacacgat aaacgtatag cgtctgaatt ctcatttaac aatgcggctg gcaaacaaat
     1561 gacacattgt acttcatgtt tgaacttcgt tacaatcatc gtgtcatttt gataaataac
     1621 aatccctcgt aattgattaa gtatatttat gataaaaatg aatattgata atctagtcaa
     1681 atttatttct ttgctcgctt cacttataat tgacatgtgc ataataagcc ctcgaaattt
     1741 tgtataattt gtcaaaagtt cagtaatctt attaatatca gacattgtga ttttttcttt
     1801 taagtcatca ttctcattaa tcaaatctac atcacatata attttagaac gtgcaataga
     1861 cacctgttct atttgtctta gcggtaagat aaaatagtct attagatgat caatttcttt
     1921 ataatagcgc acattcaact catttggttc cagatgatat attaattcaa atactcctat
     1981 ataattatct attaaacgtt tcagtttttg aattgcagta gtatttaagt cgttaattaa
     2041 aaatccaatc ttacatctat tactctcaaa agtcatatta aaaattgtag ctactagctt
     2101 ttctatcttg cctctttcag tactcgttaa caattcctta tattcatgtt taatacgaaa
     2161 tactacattt acattgtaat tcactatctt ttgaagtaat aaagtgattt gttcaatcga
     2221 tagctcatta cttgtgtcga ttgtaacaat tatatgcaag ttttcaggat taacacttaa
     2281 tctttgaatg atttgtttaa tagtataata atccatccaa taaaaattac ttcctttaat
     2341 atagatgttt ttaggttgat acatttgact ccttttggta ggctcaaaag gtatatcaat
     2401 ctcgcgcata cttgaagaac tttgattagt atcatcaaat aattcaatta tatttttatc
     2461 aatttcaact aattgggaat ggtaaattga atgagatgtt ggactatatc tttttctaat
     2521 taatttagga gtgtcaccat acgtttcttt aaagaggtgt ataaaacgag aataatgatt
     2581 aaaaccatga ctacttgcga tttcttcaat gggcttctta gaagttaaaa tatcaattaa
     2641 gcaatactcc agtctaatat gatttaaata ttgaataaaa ttactataag gtgtcgcttt
     2701 aaacatgtca cttaaagctt tgtttgtaat actaacttga ttaatgacat ctttcctatt
     2761 tatcttttta tggtggttgt ttgttaaata atcgtgcact tcttcggcta ctaaatgacg
     2821 actaccatcg tatgtattta atgacattaa ttcaacacac atgtaattaa taatcttatc
     2881 attagcatta taaggctgtt gtttaatttt gctgtaaatt aaatacttaa tcaagattct
     2941 aaaagtgcta gcaacctcat ttgttaatat cttgccacgc atcatatagt ttgcattcat
     3001 aaacttcaaa aatgtctttg cacttacttc aacaacacaa catacactat catcatgacc
     3061 atcaatctga tacaaatcat tcaacataat aacggtgaca tcatttcttt ttacatcata
     3121 ttgttgtaga ttgattgaaa ctacacctgt accttgtagc caatatataa tttttaagtt
     3181 tgcttccttc acactactca ttttcatact tttaaaaata tgtagtgaat aattatctgt
     3241 catcttacat cctcacatat acaaatatat tgatttactc ctgtttgtct ttctcaaatc
     3301 aattgtcaac atctaatatt cactaccaat tatatcgcca catgtttttt agcagtaaca
     3361 aaacataaat
//
LOCUS       DAHHOJ010000169         3350 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.172, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000169 DAHHOJ010000000
VERSION     DAHHOJ010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3350)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.172"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(181..2364)
                     /locus_tag="NBH32_002540"
     CDS             complement(181..2364)
                     /locus_tag="NBH32_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HCD1516107.1"
                     /translation="MTENKGSSQPKKNGNNGGKSNSKKNRNVKRTIIKIIGFMIIAFF
                     VVLLLGILLFAYYAWKAPAFTEAKLQDPIPAKIYDKNGELVKTLDNGQRHEHVNLKDV
                     PKSMKDAVLATEDNRFYEHGALDYKRLFGAIGKNLTGGFGSEGASTLTQQVVKDAFLS
                     QHKSIGRKAQEAYLSYRLEQEYSKDDIFQVYLNKIYYSDGVTGIKAAAKYYFNKDLKD
                     LNLAEEAYLAGLPQVPNNYNIYDHPKAAEDRKNTVLYLMHYHKRITDKQWEDAKKIDL
                     KANLVNRTAEERQNIDTNQDSEYNSYVNFVKSELMNNKAFKDENLGNVLQSGIKIYTN
                     MDKDVQKTLQNDVDNGSFYKNKDQQVGATILDSKTGGLVAISGGRDFKDVVNRNQATD
                     PHPTGSSLKPFLAYGPAIENMKWATNHAIQDESSYQVDGSTFRNYDVKSHGTVSIYDA
                     LRQSFNIPALKAWQSVKQNAGNDAPKKFAAKLGLNYEGDIGPSEVLGGSASEFSPTQL
                     ASAFAAIANGGTYNNAHSIQKVVTRDGETIEYDHTSHKAMSDYTAYMLAEMLKGTFKP
                     YGSAYGHGVSGVNMGAKTGTGTYGAETYSQYNLPDNAAKDVWINGFTPQYTMSVWMGF
                     SKVKQYGENSFVGHSQQEYPQFLYENVMSKISSRDGEDFKRPSSVSGSIPSINVSGSQ
                     DNNTTNRSAHGGSDTSANSSGTAQSNNNTRSQQSRNSGGLTGIFN"
     gene            complement(2361..3038)
                     /gene="recU"
                     /locus_tag="NBH32_002541"
     CDS             complement(2361..3038)
                     /gene="recU"
                     /locus_tag="NBH32_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HCD1516108.1"
                     /translation="MLCIINNVYYQTCVVKFMNYPNGKPYRKNSAIDGGKKTAAFSNI
                     EYGGRGMSLEKDIEHSNAFYLKSDIAVIHKKPTPVQIVNVNYPKRSKAVINEAYFRTP
                     STTDYNGVYQGYYIDFEAKETKNKTSFPLNNIHDHQVEHMKNAYQQKGIVFLMIRFKT
                     LDEVYLLPYSKFEIFWKRYKDNIKKSITVDEIRKNGYHIPYQYQPRLDYLKAVDKLIL
                     DESEDRV"
BASE COUNT         1012 a          577 c          539 g         1222 t
ORIGIN      
        1 attcaataat tttacatagc taaagatgtg aatatgaaag actatttaga tgtttcaaaa
       61 tgtatgtgaa tgtctgattt tacggtttct actaatatag caatagcgta tgaatactta
      121 taaaaaaatc ctcagctaca ccataaatat ggtataactg aggattttat gttgagtgga
      181 ttagttgaat atacctgtta atccaccgct gtttctagat tgttgagatc tagtattgtt
      241 atttgattgt gcagtaccac tgctgtttgc tgatgtgtca ctaccaccgt gtgcactacg
      301 atttgtagtg ttgttatctt gactaccaga aacattgatt gatgggatac taccacttac
      361 tgagctagga cgtttaaagt cttcgccatc tctagatgaa atttttgaca tcacattttc
      421 atataagaac tgtggatatt cttgttggct atgtcccaca aatgagtttt caccatattg
      481 tttaactttg ctgaagccca tccacactga catagtgtat tgaggtgtaa agccgttaat
      541 ccacacgtct ttcgctgcat tatcaggtaa attatattgt gaataagttt cagcaccgta
      601 agtaccagta cctgtcttag cacccatatt tactccagat acaccatggc catatgcaga
      661 accatatggt ttaaatgtac cttttagcat ctcagctaac atgtatgcag tgtaatcact
      721 catcgcttta tggctagtat gatcgtattc gattgtttca ccatcacgag taactacttt
      781 ttgaattgaa tgcgcgttgt tataagtacc accgttagcg attgcagcaa atgctgatgc
      841 taattgtgtt ggtgagaatt ctgaagcaga accaccaagt acttcagatg gaccaatatc
      901 gccttcgtag tttaagccaa gtttggcagc gaatttctta ggtgcatcat taccagcatt
      961 ttgcttaact gattgccaag cttttaaagc tgggatattg aaactttgtc gtaaagcatc
     1021 ataaattgat acagtaccgt gactcttcac atcgtagttt ctaaatgtag agccatcaac
     1081 ttgatatgaa gattcatctt gaatcgcatg gtttgttgcc catttcatat tttcaatggc
     1141 aggtccatac gctaagaaag gttttaaaga tgaaccagta gggtgtggat ctgttgcttg
     1201 gtttctgtta acgacgtctt tgaaatcacg tccaccagat atagcaacta aaccaccagt
     1261 tttactatca agaatcgttg caccaacttg ttggtcttta ttcttgtaga agctaccatt
     1321 atcaacatca ttttgtaatg ttttttgaac atctttatcc atgtttgtat aaattttaat
     1381 accactttgt aatacattac ctaaattttc atctttgaat gctttattat tcattaattc
     1441 agattttaca aagttaacgt atgaattata ctcagaatct tgatttgtat caatgttttg
     1501 acgttcttca gcagtacgat ttactaagtt tgcttttaaa tcgattttct tagcatcttc
     1561 ccactgttta tctgtaatgc gtttatgata atgcattaag tataaaacag tgtttttacg
     1621 atcttcagca gcttttggat gatcataaat attatagttg tttggaacct gaggtaaacc
     1681 ggctaaataa gcttcttccg ctaagtttaa atcttttaaa tctttattaa agtaatactt
     1741 agcagcagct ttaatacctg ttacgccatc agagtagtaa attttgttta gatatacttg
     1801 gaagatatca tctttactat actcttgttc taaacgatat gataagtatg cttcttgagc
     1861 tttacgtcca atagatttat gttgtgataa aaatgcatct ttaacaactt gttgtgttaa
     1921 tgttgaggca ccttcagaac caaatccacc agtcaagttc ttaccaattg caccgaataa
     1981 acgtttataa tcaagtgcgc cgtgttcgta gaaacgattg tcttcagttg caagtactgc
     2041 gtctttcatt gatttcggca cgtcttttaa atttacatgc tcatgtcttt ggccattatc
     2101 taatgtttta acaagttctc cgttcttgtc atatatcttt gcaggaatcg gatcttgtaa
     2161 tttagcttcg gtaaaagcag gtgctttcca agcataataa gcaaacaata agatacctag
     2221 taaaagaaca acgaaaaatg caataatcat gaagccaata atcttaataa tcgttctctt
     2281 cacatttcta ttcttttttg agttggattt cccaccatta ttaccatttt tcttaggctg
     2341 agaagatcct ttgttttccg tcatacgcgg tcctcacttt catctaatat caacttatca
     2401 actgctttta gatagtctaa tcttggttga tactgataag gaatatggta accatttttt
     2461 cgtatttcat caactgttat agacttttta atattatctt tatatctctt ccaaaatatt
     2521 tcgaattttg aatagggtaa aagataaact tcatctagcg ttttaaaacg aatcattaaa
     2581 aacacaatac ctttttgttg atatgcattt ttcatatgtt cgacttgatg atcatgaata
     2641 ttatttaaag gaaaggacgt cttgttttta gtttcctttg cttcaaaatc aatataataa
     2701 ccttgataaa cgccgttgta atcagttgtt gaaggtgtac gaaaataagc ttcgttaatc
     2761 acagctttac tccgtttggg atagttgaca ttaactattt gtaccggcgt aggctttttg
     2821 tgaataactg caatgtcgct tttaagataa aacgcatttg aatgttcgat atctttttca
     2881 agtgacatac cacgtccacc atactcaata ttactaaagg cagcggtctt tttccctccg
     2941 tctatagcac tatttttacg atatggttta ccatttggat aattcataaa tttcaccaca
     3001 caagtttggt agtaaacgtt gtttattata cacaacatta tagtgaaaca ccaaacttaa
     3061 attagtataa atgcgaaaga cacaatacac caaagcttat tttaacgtga tttcagcatt
     3121 aacacaataa tattataata catttaccgt taatgttgga attttacaca caattttaat
     3181 taattgaaaa gttttgctta taaaaatgaa tactcattac aatatttttg aattttgtta
     3241 taaaagtcga atcatgatca gtaattatta tttttgaggc aagtacaagt atgtgttgtt
     3301 aatcagcagg ggtgtatttg atttaaatga ttttattaga ttataagtat
//
LOCUS       DAHHOJ010000170         3322 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.173, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000170 DAHHOJ010000000
VERSION     DAHHOJ010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.173"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..120
                     /locus_tag="NBH32_002542"
     CDS             <1..120
                     /locus_tag="NBH32_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516109.1"
                     /translation="DSQLDGNVTAKELSESFKELVEEFESIEKAKKNSNSKDK"
     gene            182..442
                     /locus_tag="NBH32_002543"
                     /pseudo
     CDS             182..442
                     /locus_tag="NBH32_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001282612.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
     gene            491..838
                     /locus_tag="NBH32_002544"
                     /pseudo
     CDS             491..838
                     /locus_tag="NBH32_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002456552.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            849..1043
                     /locus_tag="NBH32_002545"
     CDS             849..1043
                     /locus_tag="NBH32_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516110.1"
                     /translation="MRIEDAYRKDVIITLLNNEEYEGFVTDYENEFESETGNLVVDIQ
                     TDFAVYSFDETEIKSIRLLK"
     gene            1084..1434
                     /locus_tag="NBH32_002546"
     CDS             1084..1434
                     /locus_tag="NBH32_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000891995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516111.1"
                     /translation="MLEMISNGKMTMKLNNVKQKRHILCTNEYNNKKNNSSLLPSYTI
                     IDSNESEKMTKKEFIDIPVLFDDEGNFRIKQVIDYKKIIGKSYVNGKYIETKLGKVHY
                     SKTGFHVVPYIKKE"
     gene            1441..1632
                     /locus_tag="NBH32_002547"
     CDS             1441..1632
                     /locus_tag="NBH32_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LSM domain protein"
                     /protein_id="HCD1516112.1"
                     /translation="MNLQSYIGKLVKLTLTNNKILIGKVIDFDDKVDNFDGYNSIEID
                     TGRITYDISENKIKTIVLQ"
     gene            complement(2181..3206)
                     /locus_tag="NBH32_002548"
     CDS             complement(2181..3206)
                     /locus_tag="NBH32_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low specificity L-threonine aldolase"
                     /protein_id="HCD1516113.1"
                     /translation="MISFENDYLEGAHEKVLKRLVDTNLVQASGYGFDQFTNQAIEKI
                     KDTIDCPNATIRFLVGGTQTNQVVINSMLESYEGVISADTGHVAVHEGGAIEYSGHKV
                     ITIPSKEGKVSASDVETYMETFKSDFKKDHMVFPGMVYISHPTEYGTLYSKSELEELC
                     KVCKQYQLPLFMDGARLGYGLMSDQSDMTIKDIAKYCDVFYIGGTKIGALCGEAIVFT
                     KNNEPKQFTTRIKHHGALLAKGRLTGIQFLELFTDNLYFNISRHAIEMANKMKDGFKN
                     KGYRLYFDSPTNQQFFILSNEKIAELEQKVKFAVWEKYDDQHRVVRFATSWATTEENL
                     NKLLELI"
BASE COUNT         1283 a          470 c          487 g         1082 t
ORIGIN      
        1 gattcacaat tagatggaaa tgtaactgca aaagaattga gtgaatcttt taaagaatta
       61 gttgaagaat ttgaaagtat agaaaaagcg aaaaagaatt caaattcaaa agataaatag
      121 catccttttt agacagataa agagaaagtg gtgctatttt tatacgcttt tttaactatt
      181 tactgaaaat gcagaagcac aaaagttaac atatttaaaa gaacttggtg aagatggcga
      241 atataaatat gttgccaaaa tagatagtaa aacatctaaa ttatgtcatt cacttaacgg
      301 aaaaatattt aaagttaaag atatgatacc aggtgtgaat gcgccaccta tgcatccttg
      361 gtgtagaagt accacagtgc cacatgttgg caattggcgg gacaagttct ttaaagagcg
      421 tgaaggtaaa tatcaagtag aagtaaaaga agcataatta caggaaaaag ctaaaaacca
      481 gatgaaagaa atgattaaaa atggtaaaat aaaaatagaa ataaattgcg gaaaataaaa
      541 tagatatatg ttaggtcatc acctatataa tgaaaataaa aaaagagcca ttttaaataa
      601 taagaaattg cctagctata caatactttc tatagattta ttgaatgaat tgttaagaga
      661 aaaaatgtca acaggcaatc taatattaag tgatgagcga tttgatttga aagagattat
      721 taattttaat caaattattg gaaaaataca tatcgaaaat gtgtatattg aaaccagaaa
      781 gggaaaagtg cattattcga agacaggtgc tcatatagta ccttatattg ataagtaggt
      841 gaaaagtatt gagaattgaa gatgcatatc gtaaagatgt tattatcacg cttttgaata
      901 atgaagaata cgaagggttt gtaactgact atgaaaatga attcgagagc gaaacaggaa
      961 atcttgttgt agatatacag actgattttg ctgtttattc gtttgatgaa actgagataa
     1021 aaagtattag attattaaaa taattacaaa atagcatcct ttctacaaaa agctaaaaaa
     1081 gaaatgcttg aaatgataag taatggtaaa atgacgatga aattgaataa tgttaagcaa
     1141 aagcgtcata ttttatgcac aaatgaatat aataataaga aaaataattc atctttatta
     1201 ccaagttata ctataataga ttctaatgaa agtgaaaaga tgactaaaaa agaatttatt
     1261 gatattccag ttttgtttga tgatgaaggg aattttagaa taaaacaagt tatagattat
     1321 aaaaagataa taggtaaatc ttatgtgaat ggtaaatata ttgaaacgaa attaggtaag
     1381 gttcattatt ctaaaactgg ttttcatgta gttccgtata ttaaaaagga gtgattaaat
     1441 atgaatttac agtcatacat aggcaaatta gtcaaattaa cgcttactaa taataaaata
     1501 ttaattggaa aagtgataga ctttgatgat aaagttgata attttgatgg ttataattcg
     1561 atagaaattg atacaggtag aattacatat gatatatcag aaaataaaat taaaactatt
     1621 gttttacaat aatagcatcc tttctacaca gataaagaga aagcggtgct attttttgcg
     1681 cttttataac aactaaggat acaagacaca taacttcaac aatatcctca tctaaaccta
     1741 ttgatttcct aagttcaatt ttaaacacaa agtttatttg taaagatgca cacctataaa
     1801 aatcttaatc atcgaaatgt cacaaacatc tccatattaa aaacttaaat agtgcattcc
     1861 atttaaacta aacatcgata ttcctaccaa aatagattta gtaattcaaa tgatattaca
     1921 tcactttcat aaaataacta caaccatcat aatacttttc aaatctcgtc aaattaaagt
     1981 aaatacagca caaattaaca tttttaaatt tataaaataa aacaagatac tcaataatct
     2041 gtagtatctt gttttggaat aatgtaattt tatatcatca acggcttcaa atgagcagtt
     2101 tcatatataa ataaacacaa ctactcaact gaacttgatt aattaataaa tgatttgcca
     2161 gatgacatat aacagcatta ttagattaat tctaatagtt tatttaaatt ttcttcggtt
     2221 gtcgcccagc tggttgcgaa tctaacaaca cgatgttgat catcgtattt ttcccaaaca
     2281 gcaaatttaa ctttttgttc taactctgct attttctcgt tacttaaaat aaaaaattgt
     2341 tgattggttg gagaatcaaa gtaaagacga tagcctttat ttttaaaccc gtctttcatc
     2401 ttatttgcca tttcgatagc atgtctgctt atattaaaat ataaattgtc cgtaaataat
     2461 tctaaaaatt gtatgcctgt taaccgtcct tttgctaaaa gggcaccgtg atgcttgatt
     2521 cgagtggtaa attgtttcgg ttcattattt ttcgtaaaaa caatggcttc cccgcataat
     2581 gcacctatct tcgtaccacc tatataaaat acatcacaat atttagcgat gtctttaata
     2641 gtcatatctg attggtcact catcaatcca taccctaatc gtgcaccatc cataaataat
     2701 ggaagctgat attgcttaca tactttgcat aactcttcca attctgattt agagtataat
     2761 gtgccatatt ctgtaggatg agaaatatat accattcctg ggaataccat atggtccttt
     2821 ttaaaatcac ttttaaatgt ctccatgtaa gtttcaacat ctgaagcact aacttttcct
     2881 tccttagagg gtatagtaat tactttatgt ccactatatt caattgcacc gccctcatgc
     2941 acagcaacat gaccagtgtc tgctgaaata accccttcgt aactttctaa cattgaatta
     3001 ataacaacct gattggtttg cgttccacct actaaaaaac ggatagtagc atttggacag
     3061 tcaattgtat ccttaatctt ttcaattgcc tgatttgtga attgatcaaa gccatatccc
     3121 gaagcttgta caagatttgt atctactaat ctttttaata ctttttcatg agcgccttct
     3181 aaataatcat tttcaaatga aatcactaca tttcccccta aaactaatat caacatttta
     3241 ataagataaa ccaatttcaa aactagttcg atatttaaaa tgtattatga atggttaaag
     3301 tttgtatcgc attatcgcga ag
//
LOCUS       DAHHOJ010000171         3241 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.174, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000171 DAHHOJ010000000
VERSION     DAHHOJ010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.174"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            26..544
                     /locus_tag="NBH32_002549"
                     /pseudo
     CDS             26..544
                     /locus_tag="NBH32_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001558444.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            597..881
                     /locus_tag="NBH32_002550"
     CDS             597..881
                     /locus_tag="NBH32_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001581821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516114.1"
                     /translation="MTEFTPFFDGSNQLNYLKFVILRFHGISKRKICKMYNFAYFRIL
                     EVCEMAKKNDYRFTYKDYKFLKQYGVSNTFICKMYHISIEDLEFFEVMNR"
     gene            1032..1352
                     /locus_tag="NBH32_002551"
     CDS             1032..1352
                     /locus_tag="NBH32_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF961 family protein"
                     /protein_id="HCD1516115.1"
                     /translation="MKPVLDIKKTLGQLNFLGVDEKYKYENGERTDKTVYAYKLASQE
                     QGEQITVKTPNKVELNYLQECELVEPDVKMYVQMSGDFGTIAYSWNAEDIKVVGSNSA
                     LKQK"
     gene            1366..1668
                     /locus_tag="NBH32_002552"
     CDS             1366..1668
                     /locus_tag="NBH32_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000386891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516116.1"
                     /translation="MDYYTADRLYRYTNSSNLSEPILNYVASRINWGDKVSLMTLAKE
                     IQSKFNDSYVKENTVKGRPKIYADLCLLCMSLSEAGHGRMLQVNLEDCIYIGDIDV"
     gene            1843..2934
                     /locus_tag="NBH32_002553"
     CDS             1843..2934
                     /locus_tag="NBH32_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000172946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HCD1516117.1"
                     /translation="MTLKSQCTPLTNRGVERTNKSAVEAVVDWVQVTFHIAPISAVIE
                     DVIGLPITLFKKRNSGIYFYNRGYEFSNIKLYYSSDDESMGIHLQLTGTGCREFEHHL
                     QQLNKTWQDFFDKCLSVNANFTRIDIAIDDYKTYLKVPLLIKKAEKAECVSRFRAGSA
                     INGFNLSDGRSKGATFYIGSKQSNLYCRFYEKNYEQAFKRHCDVEDIGLWNRYEIQMR
                     KAYAVNCAKVLSRTDNISEIVKSILHNNLRFISPPKDGNDKNRKRWPLYRPWALFIKD
                     TEKLNLTTRPTLKSIEDNLDWLCKQVATTLDTVLTAESMAQSEGLLTDTDFLDKILAH
                     SQFNDEHTNRINHYLEALKQKKHLSKDKC"
     gene            2995..>3241
                     /locus_tag="NBH32_002554"
     CDS             2995..>3241
                     /locus_tag="NBH32_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000691999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HCD1516118.1"
                     /translation="MKIISKYILTRFSKNGKHFDIPKEQRRVIPKFKNRRKMIVICFY
                     SLLAILLILLLASFIKATRANNDSKEAVNKTNMIQKKY"
BASE COUNT         1165 a          381 c          591 g         1104 t
ORIGIN      
        1 gcaagtaagt gaggtagtta aaaaaatgaa tcgaattgaa aaacatgcta aaaacacttt
       61 tattattctg atgctcatta tgttattttg gatttttatg agttttattt tccagaaatt
      121 attatttcca ccttcgaaaa ataatttaac tacatatgaa gcattgaaat attatacaca
      181 tttaaaggga tattacggtt tggatcatat atcaaaagga atagcataca ttgcctgtgt
      241 gttaattcca ctcaatttct tttttagatt caatgacatc aaaaaagata ataactataa
      301 taatattata agtacgttat tcctattatt atacttttta gttaatggaa tttcattgat
      361 tattcaagga ttcactgcag aatttacaat tagtttaatt agtaaggcaa atattcataa
      421 taatcatgaa tttgctgtga acttatttag atatgtcata caagaaggtg gtatttcttt
      481 tagtacatat ctagtgtgta acttttgcat aataatgtgg ttattatttt cgtgttcttt
      541 attaggtggt gtaaactcta gttaacagtt aattcttatg aaaggaattg ataattatga
      601 ctgagtttac accttttttt gatggttcaa atcaattaaa ttatcttaaa tttgtaattt
      661 taagatttca tggtatttct aaaagaaaaa tttgtaaaat gtataatttt gcatattttc
      721 gtatattaga agtttgtgaa atggcaaaaa agaatgatta tcgatttact tataaggatt
      781 ataaattttt aaaacaatat ggtgtgtcta atacatttat ttgtaagatg tatcatattt
      841 caattgaaga cttagaattt tttgaggtga tgaaccgata ggtttgatta aaaatatgat
      901 aggtcaggag aagcatagac actccattat caaaaatgca gtggtacgga tttgcgggag
      961 ttattcctat tcgtgcgacc ctttagaagt cgtttaattt tattaaaaat tttgtaaagg
     1021 atggatatat tatgaaacca gtattggata ttaaaaaaac tttaggacaa ttaaactttt
     1081 taggtgtaga tgaaaaatat aaatatgaaa atggagagcg tactgataaa actgtttatg
     1141 catataaatt agcgagtcaa gagcaaggcg aacaaatcac tgttaaaacg cctaataaag
     1201 tagaattaaa ctatttacaa gaatgcgaat tagttgaacc agacgttaaa atgtatgttc
     1261 aaatgtcagg cgattttgga actatcgcat atagctggaa tgcagaagat attaaagtag
     1321 ttggttctaa tagtgcactt aaacaaaaat aattaggtga tgtaaatgga ttattataca
     1381 gcagatagat tatatcgcta cacaaatagt tcaaatttga gtgaacctat tttaaactat
     1441 gttgctagtc gtataaattg gggcgataaa gtttcattaa tgactttagc taaagaaata
     1501 cagtctaaat ttaatgatag ttacgttaaa gaaaatacgg taaaaggtcg gcctaaaatt
     1561 tacgctgacc tttgtctatt gtgtatgagc ttgagtgaag caggacatgg cagaatgcta
     1621 caagtgaatt tggaagattg tatttatatt ggcgatattg atgtttaaca tatagtatta
     1681 gtgatttaac atgaccgtgt agcttttagc gtatgcaggc gcagaaatgc aagcacggag
     1741 tgcgttaagc atttgggcgg agcctgcgac aaatacgcgt taagaaagca agaacggtca
     1801 tgtatcaaat cgcttgcgat ttgataggag gcataaataa atatgacttt aaaaagccag
     1861 tgtacccccc ttactaatag gggggtagag cgtacaaata aaagtgccgt ggaggcagtt
     1921 gttgactggg ttcaggtcac tttccatata gcccctattt cagcagtgat tgaggacgtt
     1981 atcggtttgc ctataacgtt gttcaaaaaa cgaaatagtg gcatttactt ttataatcgt
     2041 ggttatgaat tttcaaacat taaattgtat tattcaagtg atgatgaatc aatgggcatt
     2101 cacttacagt taactggtac aggttgtaga gagtttgaac atcatttaca acagttgaat
     2161 aaaacttggc aagatttttt tgataaatgt ttatcagtta atgcaaattt tacaagaatt
     2221 gatattgcaa ttgatgatta taaaacatat ttaaaagtgc ctttattaat taaaaaggct
     2281 gaaaaagctg agtgtgtttc acgttttaga gctggaagcg caataaacgg ttttaatttg
     2341 tcagacggaa gaagtaaagg cgctactttt tatattggtt ctaaacaaag caatttatat
     2401 tgtcgttttt atgaaaagaa ttatgaacaa gcgtttaaac gtcattgtga tgtcgaagac
     2461 attgggttat ggaatcgtta tgaaatacaa atgcgaaagg catatgcagt taattgtgcg
     2521 aaagttttga gtagaacaga taatataagc gaaattgtaa agtctatatt gcataataat
     2581 ttgcgtttta tctctcctcc taaagatgga aatgataaaa atcgtaagcg gtggccatta
     2641 tatagacctt gggcgttatt tattaaagat actgaaaaat tgaatttaac tactagacca
     2701 acattaaagt ccattgagga taatcttgat tggttatgta aacaagttgc tactacttta
     2761 gatacagtgt taactgccga aagtatggcg caatctgagg ggttgcttac agatactgat
     2821 tttttagata aaattttagc gcattcacaa tttaatgatg agcatacgaa tagaattaat
     2881 cattatttgg aggctttaaa acaaaaaaag cacttatcaa aagataagtg ttaaaaaaat
     2941 aactgggatg tgatttttat taaaaatttc gtaaaggatt ttagaaaatc gtttatgaaa
     3001 ataataagca agtacatttt aacacgattt tctaaaaacg gaaagcattt tgatattcct
     3061 aaagaacaac gccgagtaat tccaaaattt aaaaatcgtc gtaagatgat tgtaatttgt
     3121 ttttactcat tattagctat tttgttaatt cttttattag cttcttttat taaagctaca
     3181 agagcaaata atgacagtaa agaagcagtt aataaaacga atatgattca aaaaaagtat
     3241 g
//
LOCUS       DAHHOJ010000172         3128 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.175, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000172 DAHHOJ010000000
VERSION     DAHHOJ010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.175"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..622
                     /locus_tag="NBH32_002555"
     CDS             <1..622
                     /locus_tag="NBH32_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000581879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1516119.1"
                     /translation="LDDFYDKEGFRDQEFDKGDKGTWIISSEMNIQLKGESLKSRGMI
                     LYMNRNTRRTTGYFFVSETKGKTYEKERKYPVKMEHNQIIPTKPIQDDKLKKEIENFK
                     FFVQYGNFKDINDYKNGDISYNPNVPSYSAEYQLRNDDYNVQQLRKKYDIPTKQAPKL
                     LLKGDGDLKGSSVGSKELEFTFVENKKENIYFSDNIDYKPTERDEL"
     gene            619..1893
                     /locus_tag="NBH32_002556"
     CDS             619..1893
                     /locus_tag="NBH32_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516120.1"
                     /translation="MMKSDYQINSGNITSHSEETSAVSKIGYEIENANNNNLGVEKIR
                     RQIVKLQKKDSFPKNLKYVDSYTDPKTGTTTSAFLNKDTGKVTLGMTGTNVNKDASET
                     LKDFGADVNIGLHIVTDKDPHYKNTQDFIKNIKKDYDIDIITGHSLGGRDAMILGMSN
                     DIKHIVVYNPAPLAIKDVSGLYADEDELKKLIEKYDGHIVRFVSDEDELDAVVRNHLY
                     ETAGEKIVLKNGEGHSMSGFLISGTQAIILAELNKVKGYQDENNKSLKSVRKQTRHRL
                     HKVETLRANWIQTTGGSLSSSQQQLLEALTALTIAEGLNQLVNEESQHLKKMYHAMAH
                     KFGDNWKKTQEVGNEIGEKLTSEEVIDELRKGGAYESKLETDPKRKIDDKIKKLNDVY
                     KNCNGYIAKIKQSIEAIVSNDQMLASQIDGMM"
     gene            1893..2219
                     /locus_tag="NBH32_002557"
     CDS             1893..2219
                     /locus_tag="NBH32_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516121.1"
                     /translation="MFTTYKNINELENAYDEERKQLNDAFNQIDELRHQTRKKCEQMY
                     DHFLYLKHKMNYSEDAMIRMTRIIESFDRETNQRIRHHEMKLEDYKDELRREYLKQSD
                     RIEGDE"
     gene            2222..2509
                     /locus_tag="NBH32_002558"
     CDS             2222..2509
                     /locus_tag="NBH32_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516122.1"
                     /translation="MGLDFSGLPDLAVLEQMKEKEQISEVIAPEHVRMHHDHQNKLKS
                     DEKILLDQMVSHFKKFEDDFKNAAQGAWVKNATDELKDISNDLEKIQDIKV"
     gene            2826..2933
                     /locus_tag="NBH32_002559"
                     /pseudo
     CDS             2826..2933
                     /locus_tag="NBH32_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000649608.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Csa1 family protein"
     gene            3045..>3128
                     /locus_tag="NBH32_002560"
     CDS             3045..>3128
                     /locus_tag="NBH32_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1516123.1"
                     /translation="MIHSKKLTLGICLVLLIILIVGYVIMTK"
BASE COUNT         1284 a          373 c          577 g          894 t
ORIGIN      
        1 tctcgatgat ttttacgata aagaaggctt tcgagatcaa gaatttgaca aaggagacaa
       61 aggtacttgg ataatcagct ctgaaatgaa tattcaatta aagggagaaa gtttaaaatc
      121 aagaggaatg attctatata tgaatagaaa tacaagaagg acaacaggtt atttttttgt
      181 aagtgaaaca aaaggaaaaa cgtatgaaaa agaaagaaaa tatccagtca aaatggaaca
      241 taatcaaatt atcccaacga agccaataca agatgacaag ttgaaaaaag aaattgaaaa
      301 ctttaaattt tttgtacaat atggcaattt taaagatatt aatgattata aaaatggtga
      361 tatttcatat aatcctaatg tgcctagtta ttcagcggaa tatcaattga ggaatgatga
      421 ttataatgtg caacagttaa gaaaaaaata tgacatacca acgaaacaag caccgaaact
      481 gttattaaaa ggcgatggtg atttaaaagg atcatctgta gggtctaaag agttagaatt
      541 tacttttgtc gaaaataaaa aagaaaatat ttacttttct gataatattg attataaacc
      601 gactgagcga gatgaattat gatgaaatca gattatcaaa ttaattcagg taatataaca
      661 agtcattctg aagaaacaag tgcagtgtca aaaattggct atgaaattga aaatgccaat
      721 aataataatt tgggggtaga aaaaatcagg aggcaaattg taaagttaca aaaaaaggac
      781 agtttcccta aaaatttaaa atatgtcgat agttatacag acccaaaaac aggtacgact
      841 acaagtgcct ttttaaataa agacacaggc aaagttactt taggtatgac aggtactaat
      901 gtaaacaaag atgcaagtga aacgctaaag gattttgggg ccgatgtcaa tattggcctt
      961 catatagtca cagataaaga tccacattat aaaaataccc aagactttat caaaaatatc
     1021 aaaaaagact atgatattga tattattacc ggacattcgc tgggcggtag agatgcgatg
     1081 attttaggta tgagtaatga tattaaacat atcgttgtgt ataatccagc gccattagcg
     1141 atcaaagatg tgagtggtct ttatgccgat gaagatgaac ttaaaaaatt gattgaaaag
     1201 tacgatggtc acattgtaag gttcgtgtct gatgaagacg aattagatgc agttgtccgc
     1261 aatcatttat atgaaactgc tggggaaaaa atagtactta aaaatggaga aggccattca
     1321 atgagtggtt ttttaataag cggaacacag gctataatct tagccgaatt aaacaaagtt
     1381 aaaggctatc aagacgaaaa taataaatca ttaaaatccg ttcgtaaaca aacgaggcat
     1441 agattacata aagtagagac gttaagagcg aattggattc aaacaacggg tggatcactc
     1501 tcttcctccc aacaacaatt attagaagct ttaacagcac taaccattgc cgaaggctta
     1561 aatcaattag tgaatgaaga aagccaacat ttgaaaaaaa tgtatcacgc gatggcacat
     1621 aaatttggag acaactggaa aaaaacgcaa gaagttggaa atgaaattgg tgaaaaatta
     1681 acctctgaag aggttataga tgaattaaga aaaggtggcg cgtatgaaag taaacttgaa
     1741 acagatccca aaagaaaaat tgatgataag ataaagaaat taaatgatgt ttataaaaat
     1801 tgtaatggct atatcgcaaa aattaaacag agtatcgaag caattgtttc taatgaccaa
     1861 atgttagcga gccagattga tgggatgatg taatgtttac tacgtataaa aatattaatg
     1921 aacttgaaaa tgcctatgat gaagaaagaa aacaattgaa tgatgcattc aatcaaattg
     1981 atgaattaag acatcaaaca cgcaagaaat gtgaacaaat gtatgatcat ttcttatatc
     2041 tcaaacataa aatgaattat agtgaagacg ctatgatcag gatgacacgt attatagaat
     2101 ctttcgatag agaaacgaat caacgtatcc gacatcacga aatgaaatta gaagattata
     2161 aagatgagtt aagaagagaa tatctaaaac aatctgacag aattgaagga gatgaataag
     2221 cgtgggactt gattttagtg gtttaccaga tttagcagta ttggaacaaa tgaaggaaaa
     2281 agaacagatt agtgaggtta ttgcgcctga acatgttcgg atgcatcatg atcatcaaaa
     2341 taagctgaaa agtgatgaga aaatattact tgaccaaatg gtaagtcatt ttaaaaaatt
     2401 tgaagatgat tttaaaaatg cggcacaagg ggcttgggtg aaaaatgcca cagacgaatt
     2461 aaaagatatt agtaatgatt tagaaaaaat tcaagatatt aaagtataaa aaggtattaa
     2521 gaaacacaat aagtatataa tccatttaat aataaatgaa ttatatagtt cataatttcg
     2581 actataagtg gctattagcc attatttttc gggatctgtg tcaaatcgga ctaatgaatt
     2641 caataatgga agttaagcaa ccaatctttg tttaacttct tttttatttt ttggaaaata
     2701 aagttttgaa cataataaaa tttgattatg ttttaacgaa ttttgatgtt tcttgaacta
     2761 tatatcatct agtcgtcatt tacaattggt aaatatgact tcaaactgta tgaaggtgat
     2821 ggcgattaaa aggctcatcc gtaggttcta aagaactaga atttaacttt ataagaaata
     2881 aagaagaaaa tgtgtatttt tctgatagta ttaatttcaa acctactgaa tagagttaat
     2941 agaagtttga aataaaaatt tcataaagtg ttcaaaataa tttttcgctt tagttgtaaa
     3001 tattaaaaac tgagtcaata ttaaaaatat aaggtggaga aatgatgatt cattcaaaga
     3061 aattaacgct cggtatatgc ttggtattac tcattatatt gattgtaggt tatgtcatta
     3121 tgacaaaa
//
LOCUS       DAHHOJ010000173         3096 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.176, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000173 DAHHOJ010000000
VERSION     DAHHOJ010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3096)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3096)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3096
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.176"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..835)
                     /locus_tag="NBH32_002561"
     CDS             complement(<1..835)
                     /locus_tag="NBH32_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HCD1516124.1"
                     /translation="MKIGDISIHYLNGGNTKMDGGAMFGVVPKPLWSKQYNANERNQI
                     NLPTHPILIQTAQYNLIIDAGIGNGKLSEKQLRNFGVDEESHIIADLANYNLTPKDID
                     YVLMTHMHFDHAAGLTDQAGHAIFENAIHVVQQDEWHEFIAPNIRSKSTYWDKNKGDY
                     SNKLILFEKHFEPVPGIKMQHSGGHSFGHTIITIESQGDKAVHMGDIFPTTAHKNPLW
                     VTAYDDYPMQSIREKERMIPYFIQQQYWFLFYHDENYFAVKYSDDGENIDAYILRETL
                     VD"
     gene            complement(1305..1949)
                     /gene="trmB"
                     /locus_tag="NBH32_002562"
     CDS             complement(1305..1949)
                     /gene="trmB"
                     /locus_tag="NBH32_002562"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCD1516125.1"
                     /translation="MRVRYKPWAEDYLKDHPELVDMDGQHAGKMTEWFDKTQPIHIEI
                     GSGMGQFITTLAAQNPHINYISMEREKSIVYKVLDKVKEMSLTNLKIICNDAIELNEY
                     FKDGEVSRIYLNFSDPWPKNRHAKRRLTYHTFLALYQQILNDEGDLHFKTDNRGLFAY
                     SLESMSQFGMYFTKINLNLHQEDDGSNILTEYEKKFSNKGSRIYRMEAKFHSQK"
     gene            complement(1964..2755)
                     /locus_tag="NBH32_002563"
     CDS             complement(1964..2755)
                     /locus_tag="NBH32_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001602611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HCD1516126.1"
                     /translation="MEQFYQLGWTLDSAGGASGEAYMAEQDGQKLFLKRNSNPFIAAL
                     SAEGIVPKLVWTKRIETGEVVTAQHWKNGRELSSNEMKQTRVAHLLKKIHNSRPLLSM
                     LKRMEMEPITPEIMLNKINASLSREVLTHHIVRKSLTYLEEHIPSLDSRFFTVVHGDV
                     NHNNWLLSDRDELFLVDWEGAMIADPAIDIGMLLYNYVPQQQWSEWLETYGVQESLNL
                     NKRMKWYTVIQSIGLVQWYEEQKRYKDMNTWLKFLNEVMNSNMFI"
BASE COUNT          980 a          602 c          408 g         1106 t
ORIGIN      
        1 tatcaactaa tgtttcacgt aaaatatatg catctatgtt ttcaccatca tcgctgtatt
       61 ttacagcaaa gtagttttca tcatgataaa acaagaacca atattgttgc tgaataaaat
      121 atggtatcat gcgttctttt tcacgaatcg attgcatagg ataatcatca tatgccgtta
      181 cccatagagg atttttatgt gcagtagttg ggaatatatc acccatatga actgctttat
      241 ctccttgact ttcaatcgta ataatcgtgt ggccaaagct atgacctcca ctatgttgca
      301 tcttgatacc cggaaccggt tcaaaatgtt tttcgaataa aatcaacttg ttactataat
      361 cgcctttatt cttatcccag taagttgatt tacttcttat attaggtgca ataaactcat
      421 gccactcatc ttgttgcaca acatgaatcg cattttcaaa aattgcatgt cccgcttgat
      481 cagtcaaacc ggcagcatga tcaaaatgca tatgtgtcat tagcacataa tcaatatcct
      541 ttggcgttaa attataattt gccaaatcag caattatatg actttcttca tctactccaa
      601 aattacgtaa ttgcttttca gataatttac cattaccaat acccgcatct ataatcaaat
      661 tatattgagc cgtttgaatc aaaattggat gtgtcggtaa attgatttga tttcgttcat
      721 ttgcattgta ttgctttgac cacaacggct tcggaacaac accaaacatt gcaccgccat
      781 ccatttttgt attgccacca tttagataat gaatagatat atccccgatc ttcataacat
      841 cacctattct ttctgtattc gttattacaa ttagcatgtg tttattacat catatcacta
      901 ttcataaatg cgaacaaaaa cgaaacgccc cacactaata cacttccaac tacttataaa
      961 gattaatgca atcatattat atgtaaatac atcataacca tcggactgta attttaaatt
     1021 aataattaaa atttccaagt aaaatgcaag ctttattctt ttaaatcaaa ggtatttagt
     1081 ataacattgt tcataggcgc aattgtctcc tttagtttag gtttggtcac tttaaattac
     1141 agaggcaatt gcgctttttt atattcaaaa tcaaaaaatc ggacagatga aaagtaaaat
     1201 actttttcta tcagtccgat ttattataga accattttac tatagaacca ttatagtacg
     1261 aaaaaacact tatgattgta tgtgtacata ccctcataag tgctttattt ttgtgaatgg
     1321 aatttcgctt ccatacgata aatacgtgac cctttattcg aaaatttctt ttcatattct
     1381 gttagaatat tactgccatc gtcttcttga tgtaaattta gatttatttt tgtaaaatac
     1441 attccaaatt gagacatact ttctaaactg taggcaaata gtcctctgtt atcagtttta
     1501 aaatgtaaat ctccttcatc atttaagatt tgttgataca acgctaaaaa cgtatgatac
     1561 gttaaacgtc gttttgcatg acgatttttt ggccatggat ctgaaaagtt caaataaata
     1621 cgcgaaactt cgccgtcttt aaaatattca tttaattcaa tggcatcatt acaaataatc
     1681 tttaaatttg ttaaactcat ctctttaact ttatccaata ctttataaac gatacttttc
     1741 tcacgttcca ttgaaatata gttaatatga ggattttgag cagctaatgt tgtaataaac
     1801 tgccccatac ccgaaccaat ttcaatgtgt atcggttgcg ttttatcaaa ccattcagtc
     1861 attttccctg catgttgacc gtccatgtca accaattcag gatgatcttt taaataatct
     1921 tcagcccatg gtttgtatcg aactctcata ttttattctc ctcttaaata aacatgttac
     1981 tattcataac ttcatttagg aatttaagcc aagtgttcat atccttatat cttttttgct
     2041 cttcatacca ttgcacaaga cctatagatt gaattaccgt ataccatttc atacgtttat
     2101 ttaaattcaa gctctcttga acaccatatg tttcaagcca ttcagaccat tgttgttgtg
     2161 gaacatagtt gtaaagcagc attccgatat caattgccgg gtctgcgatc attgcacctt
     2221 cccaatcgac taaaaatagt tcatctcgat cagataataa ccaattatta tgattcacat
     2281 cgccatgtac aacagtgaaa aaacgtgaat ctaaactcgg tatatgctct tctaaatagg
     2341 ttaatgattt tctcacaata tgatgtgtta aaacttctct tgataaagag gcattaattt
     2401 tattaagcat aatctcagga gtaataggtt ccatttccat acgctttaac atacttaata
     2461 aaggtctaga attgtgtatc ttctttaata aatgtgcaac tcttgtttgc ttcatttcgt
     2521 ttgaagatag ttcacggcca tttttccaat gttgtgctgt aacaacctcg cctgtttcta
     2581 tgcgcttcgt ccatactaat ttgggcacaa taccttctgc tgataatgcc gcaataaatg
     2641 gatttgaatt tcgttttaaa aacaactttt gtccatcttg ttcagccata tatgcttcac
     2701 cagatgcacc acctgctgaa tcaagtgtcc accctaattg ataaaactgc tccaactcgt
     2761 ccacctcact ttcaattaga aaatggctct agaaataggt ttttcaagag ccatatattc
     2821 taatttataa caccatactg gtacaaattt tatgaccaga taattatttt aaatcctcaa
     2881 ccaatgccta cattacacga ctaaatttaa atcgtattgt ctttcattga caccatacat
     2941 tctatagtca cttacttgac ataaaatgtt accgtgtcta aaactacatg tttttgtaac
     3001 ttttgtaggc gataaactct agttttcaaa ataattgcta tcccattttc atggttagca
     3061 taaatttatg aactgtaaca tttacgtact tagtaa
//
LOCUS       DAHHOJ010000174         3032 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.177, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000174 DAHHOJ010000000
VERSION     DAHHOJ010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.177"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            258..530
                     /locus_tag="NBH32_002564"
     CDS             258..530
                     /locus_tag="NBH32_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001790097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactococcin 972 family bacteriocin"
                     /protein_id="HCD1516127.1"
                     /translation="MFGTILYLTLALGLSTAAYASTEYAEGGTWSHGVGSKYVWSYYY
                     HGHKGHGATAIGKYRSFSGYTRAGVKAKASATKHNCWINRAYYNIY"
     gene            574..2538
                     /locus_tag="NBH32_002565"
     CDS             574..2538
                     /locus_tag="NBH32_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin-associated integral membrane family
                     protein"
                     /protein_id="HCD1516128.1"
                     /translation="MKWFKLILDVTIFILIAILLFVYTYKENEEILPDTKYPIAVTDW
                     NKKYSKNEIYKHINQFAKNENVAIYKSTSNYTNKNVDKDIYVFNKSKATTITPFNAKY
                     NIHYLSDDEVLKKDIKGSYFVKDKNFDVSKFINFLKEYGVTAESYKIDHMMIAVGVIK
                     QMNIVVPLSSLLIVYFIYYIFEKNINFKAYAIKYLNGFTLRKIIFENFSKKCTYWVTL
                     IITQILLTTSVLWILNYTGNLDLFILRIVLLSCLFILTISVINLWTFLMLLNLNIANM
                     IKGKQHFKTIRFINTVCKSILLVLIANVMIENTSVIKDLNKIKETEKYWNVLDDYYTI
                     EFAPYHETKQSLIDNMVRSEQLVKASEAENNAILFKPKGDSVDNDNFSPDEGNVILVN
                     NQFWSIYHKQFQPDIPIKNQKNNVEVIIPQKFHAMRNEINQAYHSWFEFVHNKNNKES
                     KLSIQFINKNDYRIFTFDARDSRHLSFIEAPIIVNVQASDLSNDFYYAMISQGGYLFK
                     NYDALVKNIEKYHLDGEISGITNYKDSVMEMYHENNLKLTVLNFSQIIIAIILIIIIL
                     FDVKYYFEQHRKLLVIKKLYGYSTLRANYQYLLINNIVVVFIGILTNVILHSQYIMMI
                     FVTIIVVQILLQICSLYYHGRRFNGVIKEF"
     gene            2541..2861
                     /locus_tag="NBH32_002566"
     CDS             2541..2861
                     /locus_tag="NBH32_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HCD1516129.1"
                     /translation="MKFIIAILLGLILSITIAFTIIHHPILDRFNPFLKTEYSYAKVP
                     KGTQQYVNITAYSERGEKLDYKLTFNGFSPSRTYVEIKHKGQYVISITYVEKEDTPKE
                     VRQE"
     gene            2858..>3032
                     /locus_tag="NBH32_002567"
     CDS             2858..>3032
                     /locus_tag="NBH32_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1516130.1"
                     /translation="MIELKDLTIQKGNTHILKKLNLKFQCGKSYALIGKSGCGKSTLL
                     NTIAGLEKTGKQYV"
BASE COUNT         1176 a          380 c          465 g         1011 t
ORIGIN      
        1 caaagaataa tggttaaagc aaacctcatt tagtcaccgt cttttaacgg gttcaatagg
       61 cgacatgttc ctgtatatcg tctatttatg cactgcgcaa tttaataata atacaattac
      121 actttgatgt ccatttaaca tgaagtagtt gaaatttgta gcaaataaat tcatcttgaa
      181 atattcagaa aattctgtat aatttaaata gagagatata gattaaatat gaagaggtgt
      241 ttttatagaa gaataaaatt tttggcacta tattatattt aactttagca cttggattat
      301 caacagcagc ttatgcatct acagaatacg cagaaggagg cacttggagt cacggtgtcg
      361 gcagtaagta tgtttggtct tattattatc atggtcataa aggacatggt gcaacagcta
      421 ttggaaaata tagatcattt agtggttata caagagctgg tgtaaaagca aaagcatcag
      481 ctactaaaca taattgctgg atcaatagag cgtattataa catttattaa atactaaaaa
      541 atcatagaaa ataaacaacg caagaggtac aacatgaaat ggttcaaatt aatacttgat
      601 gttacaatct tcattctgat tgctatattg ttatttgttt atacatataa agaaaacgaa
      661 gaaatattgc ctgatactaa ataccctata gcagtaactg actggaataa aaaatatagt
      721 aaaaatgaga tttataaaca tataaatcaa ttcgctaaaa atgagaacgt agcaatctat
      781 aaatcaactt caaattatac aaacaaaaac gtagataaag atatatatgt atttaataaa
      841 tcaaaagcaa caactatcac tccttttaat gctaaatata acattcatta tttaagtgac
      901 gacgaagtat taaaaaaaga tatcaaaggg agttattttg taaaagacaa aaattttgac
      961 gtgtctaaat tcataaattt tttaaaagaa tatggtgtta ctgctgaatc atacaaaata
     1021 gatcatatga tgattgccgt tggtgtcatt aaacaaatga atatagtagt tccattatcc
     1081 tcacttttaa tcgtttattt tatttattat attttcgaaa agaacattaa tttcaaagcg
     1141 tatgcgatta agtatttaaa tggttttaca ttaagaaaaa taattttcga aaatttttca
     1201 aagaaatgca cgtattgggt aacgttaatc ataactcaaa ttcttttaac tacaagtgta
     1261 ctttggatat taaattacac gggtaattta gatttattca tattaagaat agttctgctt
     1321 tcatgtcttt tcattttaac gattagtgtt attaatttat ggactttctt aatgttacta
     1381 aatttaaata ttgccaatat gattaaaggt aagcagcact ttaaaacaat tcgttttatt
     1441 aatacagttt gtaaaagtat tctcttagta ctaatagcta atgtaatgat agaaaatact
     1501 agtgttatta aagatttgaa taaaataaaa gaaactgaga aatattggaa tgtattagat
     1561 gattattaca cgattgaatt tgcaccttat cacgaaacaa aacaaagttt gattgataat
     1621 atggtgcgat cagaacaatt agtaaaggct agtgaagcag aaaataatgc gattttattc
     1681 aaaccaaagg gtgactccgt tgacaatgac aacttttcac ctgatgaggg gaatgtaata
     1741 ttagtaaata atcagttttg gtcgatttat cacaagcagt ttcaacctga tattccaata
     1801 aaaaatcaaa aaaataatgt cgaagtaatt attccacaaa agtttcatgc aatgcgtaat
     1861 gaaatcaatc aagcatatca ttcatggttt gaatttgtac ataataaaaa taataaagag
     1921 agtaagttat ctatacagtt tatcaacaaa aatgattatc gaatttttac atttgatgca
     1981 cgagatagtc gccatttgtc atttatagag gcgccaatca ttgtgaatgt tcaggcatca
     2041 gatttatcga atgattttta ttatgccatg atcagtcaag gcgggtattt attcaaaaat
     2101 tatgacgcgc tagtaaaaaa tattgaaaag tatcatcttg atggggaaat tagtggaata
     2161 accaattata aagatagcgt gatggaaatg tatcatgaaa acaatttgaa attaacagta
     2221 ctcaactttt cacaaatcat tatcgcaatc attttaataa ttattatttt atttgatgtg
     2281 aaatattatt ttgaacaaca tcgaaaatta ctcgtaatca aaaagctata tggttattca
     2341 acattaagag ccaattacca atacttatta ataaataata tagttgttgt ttttattgga
     2401 atattgacga atgtaatttt acattctcag tatataatga tgatatttgt aacgattatt
     2461 gtcgttcaaa tattattgca gatttgcagc ctatactatc atggccgacg ttttaatgga
     2521 gttatcaagg agttttaact atgaaattta tcattgcaat attattagga ttaatattat
     2581 ccattactat tgcttttaca atcatacatc atcctatact tgatcgtttt aatcctttct
     2641 taaaaacgga gtatagttat gccaaagtgc caaaaggtac gcaacaatat gttaatatta
     2701 cggcttatag tgaaagaggg gaaaagcttg attataaatt aacatttaat ggattttcac
     2761 ctagtagaac gtatgtcgaa ataaagcata aagggcaata tgtcatatcg atcacatatg
     2821 ttgaaaaaga ggatacacca aaagaggtaa gacaagaatg atagaattaa aagatttaac
     2881 catacaaaaa ggtaatacac atattttgaa gaaacttaat ttgaaatttc aatgtggaaa
     2941 atcatatgca cttattggta aaagtggatg cggaaaatct acattattaa atactattgc
     3001 tggacttgaa aaaacaggga aacaatatgt ct
//
LOCUS       DAHHOJ010000175         3000 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.178, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000175 DAHHOJ010000000
VERSION     DAHHOJ010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.178"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(188..2797)
                     /gene="adhE"
                     /locus_tag="NBH32_002568"
                     /gene_synonym="adhC"
     CDS             complement(188..2797)
                     /gene="adhE"
                     /locus_tag="NBH32_002568"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCD1516131.1"
                     /translation="MLTIPEKENRGSKEQEVAIMIDALADKGKKALEALSKKSQEEID
                     HIVHQMSLAAVDQHMVLAKLAHEETGRGIYEDKAIKNLYASEYIWNSIKDNKTVGIIG
                     EDKEKGLTYVAEPIGVICGVTPTTNPTSTTIFKAMIAIKTGNPIIFAFHPSAQESSKR
                     AAEVVLEAAMKAGAPKDIIQWIEVPSIEATKQLMNHKGIALVLATGGSGMVKSAYSTG
                     KPALGVGPGNVPSYIEKTAHIKRAVNDIIGSKTFDNGMICASEQVVVIDKEIYKDVTN
                     EFKAHQAYFVKKDELQRLENAIMNEQKTGIKPDIVGKSAVEIAELAGIPVPENTKLII
                     AEISGVGSDYPLSREKLSPVLALVKAQSTKQAFQICEDTLHFGGLGHTAVIHTEDETL
                     QKDFGLRMKACRVLVNTPSAVGGIGDMYNELIPSLTLGCGSYGRNSISHNVSATDLLN
                     IKTIAKRRNNTQIFKVPAQIYFEENAIMSLTAMDKIEKVMIVCDPGMVEFGYTKTVEN
                     VLRQRTEQPQIKIFSEVEPNPSTNTVYKGLEMMVDFQPDTIIALGGGSAMDAAKAMWM
                     FFEHPETSFFGAKQKFLDIGKRTYKIGMPENATFICIPTTSGTGSEVTPFAVITDSET
                     NVKYPLADFALTPDVAIIDPQFVMSVPKSVTADTGMDVLTHAMESYVSVMASDYTRGL
                     SLQAIKLTFEYLKSSVEKGDKVSREKMHNASTLAGMAFANAFLGIAHSIAHKIGGEYG
                     IPHGRANAILLPHIIRYNAKDPQKHALFPKYEFFRADTDYADIAKFLGLKGNTTEALV
                     ESLAKAVYELGQSVGIEMNLKSQGVSEEELNESIDRMAELAFEDQCTTANPKEALISE
                     IKDIIQTSYDYKQ"
BASE COUNT          859 a          569 c          471 g         1101 t
ORIGIN      
        1 aaaagaaaac catacgcata gcgtatggtt ttctttttac aaaaaaatga ttgtctagtt
       61 tgtatctctc tgaagatttg gcaataaata aaagccgata accgtataat gattatcgac
      121 ttaaagttta tgtggcattt tttacttttg taatttcagg tgagttagat gattattatc
      181 agatagatta ttgcttataa tcatatgatg tttgaatgat atctttgatt tcactgatta
      241 gtgcttcttt aggattagca gttgtacatt gatcttcaaa tgcgagctct gccattctat
      301 caattgactc atttaattct tcttcagaca caccttgtga tttcaaattc atttcaattc
      361 cgactgattg acctaattcg tagacagctt tagctaatga ttctacgagt gcttctgtcg
      421 tattaccttt taatcctaag aatttggcaa tatctgcata atctgtatct gctctgaaga
      481 actcatattt agggaataat gcatgttttt gtgggtcttt ggcattataa cggataatat
      541 gcggtagtaa tatcgcattc gctctaccat gcggaatacc atattcgcca ccaattttat
      601 gcgcaattga gtgtgcaatg cctaagaatg catttgcaaa tgccatacca gccaaagttg
      661 atgcgttatg cattttctct cttgaaactt tatcaccctt ttcaacagat gattttaaat
      721 attcgaacgt caatttaatc gcttgtagac tcaaacctct tgtgtagtct gaagccatta
      781 cagaaacata tgattccatt gcatgcgtta gtacatccat tcctgtatct gctgtaacgc
      841 tttttggcac actcatcaca aattgagggt caataattgc aacgtcaggt gttaaagcaa
      901 aatcagccaa cggatatttt acatttgttt cactatctgt gataactgca aatggtgtta
      961 cttctgaacc tgtacctgat gtcgtaggga tacaaatgaa cgtcgcattt tcaggcatgc
     1021 ctattttata agtacgttta ccgatgtcta ggaacttttg tttagcaccg aagaatgatg
     1081 tctcagggtg ttcaaagaac atccacattg cttttgcagc atccatcgct gaaccaccac
     1141 caagtgcaat gattgtatcc ggttggaaat caaccatcat ttccagacct ttatatactg
     1201 tattagttga tgggttcggt tcgacttcgc taaatatttt aatttgaggc tgttccgttc
     1261 tttgtcttaa tacattctca accgtttttg tataaccgaa ttcgaccata ccagggtcac
     1321 agacaatcat cactttttca atcttgtcca ttgctgttaa actcatgatt gcattttctt
     1381 caaaataaat ttgagcaggc accttgaaaa tttgagtatt attacgtcgt ttagcaattg
     1441 ttttaatgtt taataaatct gtcgcactaa cattatgtga aattgagttt ctaccgtacg
     1501 aaccacaacc taatgttaaa gacggaatca attcgttata catatcacca atacctccaa
     1561 ccgctgatgg tgtatttaca agtacacgac aagctttcat tcttagtcca aaatcttttt
     1621 gtaatgtttc atcttctgta tggataacgg ctgtgtgtcc taatccacca aaatgtagtg
     1681 tgtcttcaca aatttgaaat gcttgttttg tagattgggc ttttactaag gctaatactg
     1741 gagataattt ttcacgagat aacggatagt ctgaacctac accgctaatt tcagctatga
     1801 taagttttgt attttcgggg acaggtatac ctgctaattc agctatttca actgcagatt
     1861 taccgacaat atcaggctta atacctgttt tttgttcatt cataattgca ttttctaagc
     1921 gttgtaattc atctttttta acaaagtatg cttgatgtgc tttaaattca ttagtaacgt
     1981 ctttataaat ttctttatca ataactacaa cttgttcaga agcacaaatc ataccattat
     2041 caaatgtttt tgaaccaatg atatcattta ctgcacgttt aatgtgtgct gttttttcaa
     2101 tgtaagacgg cacgttacct ggtcccacac ctaatgccgg tttgccagtt gaatatgcag
     2161 acttaaccat gcccgaacca cctgttgcta gaactaatgc aatacctttg tgattcatta
     2221 attgttttgt tgcttcgata gaaggcactt caatccattg aataatatct ttaggtgcac
     2281 ctgccttcat tgccgcttct aatacaactt ctgctgcacg cttcgacgat tcttgcgcac
     2341 ttggatggaa tgcaaaaatg attggatttc ctgtcttaat tgcaatcatc gctttaaaaa
     2401 tagttgtcga cgtaggattt gttgttggcg taacaccaca aataacacca attggttccg
     2461 ctacatacgt taatcctttt tctttatctt caccaataat ccctactgtc ttattgtctt
     2521 ttattgaatt ccatatatat tcagaagcgt ataaattttt aatcgcttta tcttcgtata
     2581 tacctcttcc agtttcttca tgtgctaatt ttgctagcac catatgttga tcaacagctg
     2641 ctaagctcat ttgatgaaca atatgatcaa tttcttcttg tgacttttta gataatgctt
     2701 ctaatgcttt tttccctttg tcagctagag catcaatcat aattgccact tcttgttctt
     2761 tcgatccacg attttctttt tcaggtatag ttaacatata caaccactcc tttatacttt
     2821 gtgaattatt tcacaaacat tatagtacat gtctctcagg atataaagaa aattctatac
     2881 aaaaaagttt aatttcgaat attatttgaa caaatatcaa attttaaaat aaatgttttc
     2941 atgaaatcat tgttatttcg gtgtttttag aatgatttta taatcataat tttttcaatg
//
LOCUS       DAHHOJ010000176         2953 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.179, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000176 DAHHOJ010000000
VERSION     DAHHOJ010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2953)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2953)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2953
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.179"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..624
                     /locus_tag="NBH32_002569"
     CDS             <1..624
                     /locus_tag="NBH32_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3427 domain-containing protein"
                     /protein_id="HCD1516132.1"
                     /translation="IEKIYGSPIIECNERMIRLSDAFTNALSNQTFKIFLEDLIELSK
                     YNNEKYQKGKNGLILYNKYSREDFSKIFNWNKNGSSVIMGYMIRSQEMPIFITYDKHE
                     DISDSTKYEDEFLSQDELKWFTKSNRTLKSKEVQKILSHRAKGIKMYIFVQKKDDDGI
                     YFYYLGTAGYIEGSEKQDKMPNGSNVVTMDLALDKAVRDDIYRYITN"
     gene            930..1679
                     /locus_tag="NBH32_002570"
     CDS             930..1679
                     /locus_tag="NBH32_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1516133.1"
                     /translation="MIHSKRLRLWLYLVLLAVFISACNVKEDKQIKENFNKTLSLYPT
                     KNLDDFYDKEGFRDQEFDKRDKGTWIINSGMNIQLKGGALKSREMVLYINRNTRTAKG
                     YFLISETTEDKKGYVHNKDKKYPVKMEHNQIIPTKPIRDDKLKKEIENFKFFVQYGDF
                     KDYKDGDISYNPNVPSYSANYQLNNDDYNVQQLRKRYDISTKRAPELKLRGSGDLKGS
                     SVGSKELEFNFIRNKEGNVYFSDSINFKPTE"
     gene            1790..2575
                     /locus_tag="NBH32_002571"
     CDS             1790..2575
                     /locus_tag="NBH32_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1516134.1"
                     /translation="MMHSKKLMLGICLVLLIILIVGYVIMTKINSRSAQIKDTFNQTL
                     KLYPTKNLDDFYDKEGFRDQEFGKGDKGTWIINSGMNIQLKGGALKSREMVLYINRNT
                     RTTKGYFIVGEITKDKKGYTHDKDKKYPVKMEHNQIIPTKPIKDEKLKKEIEDFKFFV
                     QYGNFKDFKDYKNGDISYNPNVPSYSAKYQLSNDDYNVQQLRKRYDIPTKKAPELLLK
                     GDGDLKGSSIGSKDLEFIFVQNKRENIYFTDSVEFTPSEDDKS"
     gene            2833..>2953
                     /locus_tag="NBH32_002572"
     CDS             2833..>2953
                     /locus_tag="NBH32_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1516135.1"
                     /translation="MIHSKKLTLGICLVLLIILIGGYVIMTKANGRNAQIKDTF"
BASE COUNT         1235 a          331 c          492 g          895 t
ORIGIN      
        1 attgaaaaaa tctatggaag tcccattata gaatgtaatg aaagaatgat aagactaagc
       61 gatgcattta ctaatgctct aagtaatcaa acatttaaaa tatttttaga agatcttatt
      121 gaattatcaa agtataacaa tgaaaaatac caaaaaggta aaaatggact gattttatat
      181 aataaatatt caagagaaga ttttagcaaa atatttaatt ggaataaaaa tggttcgtct
      241 gtaatcatgg gatacatgat aagaagccaa gaaatgccaa tatttatcac ttatgataag
      301 catgaggata taagtgatag cacaaaatat gaagatgaat ttttaagtca agatgaatta
      361 aaatggttta ctaaatctaa tagaacgcta aaatcaaaag aagttcagaa aattttgtcc
      421 catagagcaa aaggaattaa aatgtatatt tttgtacaga aaaaagatga tgatggtata
      481 tatttttatt atttaggaac tgctggatat atagaaggtt cagagaagca agataaaatg
      541 ccgaatggtt caaacgtagt aactatggat ttagcactcg ataaagctgt aagagatgat
      601 atatatcgct atataacaaa ttaatgtaaa ccgaaaaatg aatgttagta aagatgtatt
      661 taaacatcac ttttaataag taaagacatt gagtttcagt gttattattt taagtataag
      721 cgcaatttat cttaattaat tagttggtta ggccaataaa aattataaag atatttgcgc
      781 tttttaaata atttgtgatg ttgatatgtg aaaaatacaa ctaacaatta aaagccaaga
      841 ggatttaatc ataaaattcc ctctagtatt attaatattg gaatttgaac ttaactttgc
      901 atcaaaaata aaaatggggg tgtgaaatga tgattcattc aaaaaggttg aggctctggc
      961 tgtatttagt attattagct gtttttatta gcgcgtgtaa cgtgaaagaa gataaacaaa
     1021 ttaaagaaaa tttcaacaaa acattgagtt tatatccaac caagaatctc gatgattttt
     1081 acgataagga aggttttcga gatcaagagt ttgataaaag agataaaggg acttggatta
     1141 ttaattctgg aatgaacatc caattaaaag gaggagcttt aaaatccaga gagatggtac
     1201 tttacatcaa tcgtaacact agaacagcta agggatattt tttaataagt gagacaacag
     1261 aagacaaaaa aggatacgtc cataataaag ataaaaaata ccctgtcaag atggaacata
     1321 atcaaattat tccaacgaag cccataaggg atgataagtt aaaaaaagag attgaaaact
     1381 ttaagttctt tgtacaatat ggggatttta aagattataa agatggggat atttcataca
     1441 atcctaacgt accaagttat tccgcaaact atcaattgaa taatgatgat tataatgtcc
     1501 aacaattaag aaaacgatat gatatatcaa ccaaaagggc tccggaatta aaattgagag
     1561 gatctggcga tttaaaaggc tcatctgtag gttctaaaga actagaattt aactttataa
     1621 gaaataaaga aggaaatgtg tatttttctg atagtattaa tttcaaacct actgaatagg
     1681 gttaatagaa gtttggaata aaattccata aagtgttcaa aataattttt cgctttagtt
     1741 gtaaatatta aaaactgagt cagtattaaa aatataaggt ggagaaatga tgatgcattc
     1801 aaagaaatta atgctcggta tatgtttggt attactcatt atattgattg taggttatgt
     1861 cattatgaca aaaataaata gtcgaagcgc ccaaattaaa gacacattta atcaaacatt
     1921 aaaattatat ccaaccaaaa atctcgatga tttttacgat aaggaaggct ttcgagatca
     1981 agaatttggc aaaggagaca aaggtacttg gattattaat tctggaatga acatccaatt
     2041 aaaaggagga gccttaaaat ccagagagat ggtactttac atcaatcgta acactagaac
     2101 aacgaaagga tattttatag taggtgagat aactaaagat aaaaaaggat atacacatga
     2161 taaagataaa aaataccctg tcaagatgga acataatcaa ataatcccaa caaaacctat
     2221 aaaagacgag aagttaaaaa aagaaattga agactttaag ttttttgtac aatatggcaa
     2281 ctttaaagat tttaaagatt ataaaaatgg ggatatttca tacaatccaa acgtaccaag
     2341 ttattcggca aaatatcaat taagtaatga cgattataat gtccaacaat taagaaaaag
     2401 atatgatata ccgactaaaa aagcaccaga actattgttg aaaggtgatg gcgatttaaa
     2461 aggttcatcc ataggctcta aagacttaga atttatcttt gtacaaaata aaagagagaa
     2521 tatctatttt acagattcgg ttgaatttac tccaagcgag gatgataaat cgtgagtcaa
     2581 actgaatagg gataatagaa gtttggaatt aaattttcat aaagcactag gtaaaagatt
     2641 ataacgataa ctcttaaaat taataataaa tcacagaaac tttatctttt gatttaattg
     2701 ttttataatt ctgataagaa gatttaaaat acaaaataat tacaattaaa tttatttgtt
     2761 caaaacaatt tttcgtttta gttgtaaata ttaaaaactg agtcagaatt ataaatataa
     2821 ggtggagaaa tgatgattca ttcaaaaaaa ttaacgctcg gtatatgttt ggtattactc
     2881 attatattga ttggaggtta tgtcattatg acaaaagcaa atggtcgaaa cgcccaaatt
     2941 aaagacacat tta
//
LOCUS       DAHHOJ010000177         2899 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.180, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000177 DAHHOJ010000000
VERSION     DAHHOJ010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2899)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2899)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2899
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.180"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            8..925
                     /locus_tag="NBH32_002573"
     CDS             8..925
                     /locus_tag="NBH32_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014373836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1672 domain-containing protein"
                     /protein_id="HCD1516136.1"
                     /translation="MKKFIGSVLTTTLILGGCSMMENESSKDTNTETKSVPEEMEASK
                     YVGQGFQPPAEKDAIEFAKKHKDKIAKRGEQFFMDNFGLKVKATNVVGSGDGVEVYVH
                     CDDHDIVFNASIPFDKSIIDSDSSLRSEDKGDDMSTLVGTVLSGFEYRAQKEKYDNLY
                     KFFKDNEEKYQYTGFTKEAINKTQNSGYENEYFYIVANIPTLQEYRKYYEPLIKKNNL
                     DFKKGMKDARRGVGYKAEIELYTTLFSKSRNFSKDKKLDDAIDLSESVKKLNLNLENT
                     KIFLQLTKSTINTKQVNYSDNKPIKIEVE"
     gene            928..>2899
                     /locus_tag="NBH32_002574"
     CDS             928..>2899
                     /locus_tag="NBH32_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000723487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516137.1"
                     /translation="MNNINDRDLTELSGYWVYQDIKDGSKFRVNDKKFKQVEEITKVA
                     NKNNSSDIKVYELLDENETPTGKQVLLFQGTDNQKSDVKSNPFRGKVADDWIENIKLM
                     NDKNKSTSLLEQNNAYIKSYEQKLKDADTLKSSDFLRKYKQNPSTYKNKTIKSDGGNS
                     QGGASANHQGLINPNKNIVSTNPAMLPKSIWENFKSQNFENMINYHSKFDILSWLQDP
                     FATPTLGKRVNLETGVPTMDGLVNSHLGYKRKLNPRYNTYKDLAVYKIKSVKDTMIKN
                     GKKVKKTIEIDINMDDRIPINVWTGDSIARTGKGTPIKLNLENLSALSNLVTGETSNM
                     LTDCVNYLNESYNISQVENSNFGERKHKLKQDFKKIVEVDILEGMSRELTSFKQDVFS
                     AIDDVKKNLVVIILIAPAAGVLILELNTIEEILEDLDKKLQRGIESLHDSLDNVIKEM
                     FKNLDHDFEDGVTEEMMKHLNVVNNNINFVKKQNDVYGEQITDIKNIMSHQDATVMDG
                     NLSINYSGEHMISGQVQSSNYLTRKMTILKNHIDNAVNKISDYVQETYNNYFEPVLNY
                     ILEVIHSVETAVRHIYEISSLIYKSGMKIKIKAMGINVDEIDKMLEVLKNKLKNLDEF
                     LNNLKIASPILENHLDDIVRNMKPLIVNQIFEP"
BASE COUNT         1212 a          336 c          513 g          838 t
ORIGIN      
        1 gtgtcatatg aaaaagttca ttggatcagt tttaaccacg acattaattt tagggggatg
       61 ttccatgatg gaaaatgaat caagtaaaga cacgaataca gaaacaaaat cagtaccaga
      121 agaaatggaa gcttcaaaat atgtaggaca aggcttccaa ccacctgcag aaaaagatgc
      181 gattgaattt gcgaagaagc ataaagataa aattgccaaa cgaggcgaac aattttttat
      241 ggataacttc ggtctaaaag ttaaagctac caatgttgta ggtagtggcg acggtgtaga
      301 agtttatgtg cattgcgatg accacgatat cgtatttaat gcgagtattc catttgataa
      361 atcaattatt gacagtgata gctcattaag aagtgaggat aagggcgatg atatgagtac
      421 tttagttggt acagtactaa gtggatttga atatcgagca caaaaagaaa agtatgataa
      481 cctatataaa tttttcaaag ataacgaaga gaaataccaa tatacaggat ttacaaaaga
      541 ggcaattaac aagacacaaa atagtggata tgaaaatgaa tatttttata tcgttgcaaa
      601 tataccaaca cttcaagaat atagaaaata ttacgaacct ctgattaaaa aaaataatct
      661 agattttaaa aaaggtatga aagatgctag aagaggagta ggctacaaag ctgaaatcga
      721 attatataca acactgtttt cgaaaagtag aaacttttca aaggacaaga aattagatga
      781 tgctatagat ttatccgaaa gcgtgaaaaa gttaaacctt aatttagaaa acacgaaaat
      841 atttttacaa ctaacgaaat ctaccattaa tactaaacaa gtaaattata gtgataacaa
      901 acctatcaag attgaggttg agtgaaaatg aacaatataa acgatagaga tttaactgag
      961 ttaagtggtt attgggttta ccaagatatt aaagatggta gtaaatttag agttaacgat
     1021 aaaaaattca aacaagtcga agagataacg aaagtagcta ataaaaataa tagttcagat
     1081 ataaaagttt atgaactatt ggatgaaaat gaaacaccta caggcaaaca agtattatta
     1141 tttcaaggta cagataacca aaagagtgat gtgaaaagca atccttttcg tgggaaggtt
     1201 gctgatgatt ggattgaaaa tataaagtta atgaatgata aaaataagtc aacttcttta
     1261 ttagaacaaa ataatgctta tattaaatcg tatgaacaga aactaaaaga tgcagataca
     1321 cttaaaagct ctgatttttt aaggaaatat aagcaaaatc cgtctacgta taaaaacaaa
     1381 actattaagt ctgatggagg caattctcaa gggggagcaa gtgctaacca ccaagggttg
     1441 attaacccga ataaaaatat agtttctaca aaccctgcca tgttaccaaa gtcaatatgg
     1501 gaaaatttca aatctcaaaa ctttgaaaat atgattaatt atcatagtaa gtttgatatt
     1561 ttatcttggc tgcaggatcc atttgccact ccgacactag gaaaacgagt gaatttagaa
     1621 actggtgtgc caacgatgga tggattagta aatagtcatt taggttataa aagaaaatta
     1681 aatccaagat ataatactta taaagattta gcagtttata aaattaaatc agttaaagat
     1741 actatgatta aaaatggtaa aaaagtcaaa aaaactattg aaatcgatat aaatatggat
     1801 gatagaattc caattaatgt gtggacagga gattcaatag cgagaacggg taaagggact
     1861 cctataaaat taaatctaga aaatcttagt gcattaagta atttggtaac tggtgaaaca
     1921 agtaatatgt taaccgattg tgtaaattat ttgaatgaga gctataatat ttcacaggtt
     1981 gaaaattcta attttggaga aagaaaacat aaacttaaac aagattttaa aaagattgtc
     2041 gaagttgata tccttgaagg aatgagtagg gaattaactt cttttaaaca ggatgtattt
     2101 agcgcaattg atgatgtcaa aaaaaatctt gttgtaataa tcttaatagc tccagcagct
     2161 ggagtcctta ttcttgaatt aaatactatt gaagaaattc ttgaagattt agataaaaaa
     2221 ttacaacgtg gcatagaaag tttacatgat tcacttgata acgtaattaa agaaatgttt
     2281 aaaaatttag atcatgattt tgaagatggc gtgacagagg aaatgatgaa gcatttaaat
     2341 gttgtgaata ataatataaa ttttgtgaaa aagcaaaatg atgtatatgg agagcagata
     2401 accgatatta aaaatataat gtctcatcaa gatgcaacgg taatggatgg aaacttatct
     2461 attaattata gcggtgaaca tatgatttcg ggtcaagtac aatcttctaa ttatttaacc
     2521 agaaaaatga caattttaaa aaaccatatt gataatgcag ttaacaaaat tagtgattat
     2581 gttcaagaaa cttacaataa ttattttgaa ccagtattaa actacattct tgaagttata
     2641 cattcggtag aaacagctgt gagacatatt tatgaaataa gcagtttaat atacaaatca
     2701 ggaatgaaaa taaagataaa ggcaatggga ataaatgttg acgaaattga taaaatgctt
     2761 gaagttttaa aaaataaact taaaaactta gacgaatttt taaataattt aaaaatagct
     2821 tcgccgattt tagaaaatca cttagatgat atagttagaa atatgaaacc gttaattgtt
     2881 aatcagattt ttgaaccat
//
LOCUS       DAHHOJ010000178         2747 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.181, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000178 DAHHOJ010000000
VERSION     DAHHOJ010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2747)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2747)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2747
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.181"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(727..2181)
                     /locus_tag="NBH32_002575"
     CDS             complement(727..2181)
                     /locus_tag="NBH32_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HCD1516138.1"
                     /translation="MLITKNQAEKWFDNSLGKQFNPDLFYGFQCYDYANMFFMIATGE
                     RLQGLYAYNIPFDNKARIEKYGQIIKNYDSFLPQKLDIVVFPSKYGGGAGHVEIVESA
                     NLNTFTSFGQNWNGKGWTNGVAQPGWGPETVTRHVHYYDDPMYFIRLNFPDKVSVGDK
                     AKSVIKQATAKKQAVIKPKKIMLVAGHGYNDPGAVGNGTNERDFIRKYITPNIAKYLR
                     HAGHEVALYGGSSQSQDMYQDTAYGVNVGNNKDYGLYWVKSQGYDIVLEIHLDAAGES
                     ASGGHVIISSQFNADTIDKSIQDVIKNNLGQIRGVTPRNDLLNVNVSAEININYRLSE
                     LGFITNKKDMDWIKKNYDLYSKLIAGAIHGKPIGGLVAGNVKTSAKNQKNPPVPAGYT
                     LDKNNVPYKKEAGNYTVANVKGNNVRDGYSTNSRITGVLPNNATIKYDGAYCINGYRW
                     ITYIANSGQRRYIATGEVDKAGNRISSFGKFSTI"
     gene            complement(2192..2494)
                     /locus_tag="NBH32_002576"
     CDS             complement(2192..2494)
                     /locus_tag="NBH32_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HCD1516139.1"
                     /translation="MDAKVITRYIVLILALVNQFLANKGISPIPVDDETISSIILTVV
                     ALYTTYKDNPTSQEGKWANQKLKKYKAENKYRKATGQAPIKEVMTPTNMNDTNDLG"
     gene            complement(2630..>2747)
                     /locus_tag="NBH32_002577"
     CDS             complement(2630..>2747)
                     /locus_tag="NBH32_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2951 family protein"
                     /protein_id="HCD1516140.1"
                     /translation="NKEKNAKNIRDIKMWILGLIGTILSTFVIALLKTIFGI"
BASE COUNT          843 a          544 c          407 g          953 t
ORIGIN      
        1 gtcattctct aacgtacacg agcgtccaaa aacactgttg tatcaaggtt tttgtcattt
       61 ttaacctttt agattttcca caagcgtttg ccctttttca aataatctgc ccttttttcg
      121 ccccgaaaaa acgcaaaaaa taaccacaat cctaaattaa taggatctgt ggttttgttg
      181 gttgtagggg aataaatata accgtatcga ttaagatacg gttgtagcga atgtaacatt
      241 tctatgttgt taagatatat gtatcgagtg atgacaagga agatgtctcc tgtgggacca
      301 acagtcagat acatggcctc tgcccggcta tatagttcac tcctactata taaaagtaag
      361 tataacataa aaagcacccc gtaaactgtt atacgggaat gctaaagtca tatatactac
      421 ggggagtagt atgaaaacta tgctctctat cgcaaggaaa aaacacccag tgacatgctt
      481 gggtgaacaa ggatagatgt aaatagttga tgcatgtgta acacatcata acaaaaaact
      541 agcccgaagg ctagctataa cataaaaaaa taggcaagta ccgaagtacc tgccagttac
      601 gcacatttaa atcttgagag taatgttaaa aagtgtatag gaatattaac atccatccaa
      661 atagttattt aataactgta agattcccta taattaatgt agcaaaattt ttattctaag
      721 taaatactaa atcgtgctaa acttaccaaa actgcttatt ctattacctg ccttgtctac
      781 ctctcctgta gcaatataac gacgttgtcc actattagca atataagtaa tccatctata
      841 gccattgata caatatgcgc cgtcatattt gattgttgcg ttattaggta atacaccagt
      901 aattcttgaa ttagttgaat agccgtccct tacgttatta cctttaacat tggcaactgt
      961 gtaattacca gcctcttttt tataaggcac attattctta tcaagtgtat aacctgctgg
     1021 cactggtgga tttttttggt ttttagctga tgttttaaca ttaccagcta ccaaaccacc
     1081 tataggctta ccatgaatcg caccagctat taatttagaa tacaagtcat aattcttctt
     1141 aatccaatcc atatcttttt tattagtaat aaaacctaat tcagataaac gataattgat
     1201 atttatttct gctgatacat taacgttcag taaatcatta cgaggtgtta cacctcttat
     1261 ttgtcctaag ttatttttaa taacatcttg tatactttta tcaatagtat ctgcattgaa
     1321 ttgacttgaa ataataacat gcccaccact tgcactttct cctgctgcgt ctaaatgaat
     1381 ctctagaaca atgtcatacc cctgtgattt aacccaatat aagccataat ctttattatt
     1441 tcctacatta acaccgtaag cagtatcttg atacatatct tgtgattgac ttgagccacc
     1501 atataatgca acttcgtgac ctgcatgtct taaatactta gcgatatttg gtgttatata
     1561 tttacggata aaatcacgtt catttgttcc gtttccgact gctccaggat cgttataacc
     1621 atgaccggct acaagcataa tttttttagg tttaattact gcttgctttt tggcagttgc
     1681 ttgcttaata acgcttttag ctttatctcc aacacttact ttatctggga aatttaatct
     1741 aataaaatac attgggtcat cgtaataatg aacatgtctt gtaacggttt cgggacccca
     1801 accaggttgc gcaacgccat ttgtccaacc tttaccattc caattttggc caaacgatgt
     1861 gaaagtgttt agattagcgc tctcaacaat ttcaacatgt ccagctccgc caccatactt
     1921 tgacgggaaa acgacaatgt ccaacttttg cggtaaaaag ctatcatagt ttttaattat
     1981 ttgcccgtat ttttcaatcc ttgctttatt atcaaatgga atattataag cgtataaacc
     2041 ttgtaacctt tcgcctgttg ctatcataaa aaacatattt gcgtaatcgt aacactgaaa
     2101 tccataaaac aaatcaggat tgaactgctt ccctaatgaa ttatcaaacc atttttctgc
     2161 ttggtttttt gttatcaaca ttggtcaaca cctaccctaa atcatttgtg tcgttcatat
     2221 tcgtaggtgt cattacttct ttaattggcg cttgccctgt tgcttttcta tacttgtttt
     2281 cagctttata tttctttagc ttttgatttg cccatttacc ttcttgagat gttggattgt
     2341 ctttatacgt agtatataaa gcaacaacag taagtattat tgatgatata gtctcatcgt
     2401 ctactggaat cgggctaata cctttgttcg ctaagaattg atttactaat gctaagatca
     2461 atacgatgta tcttgttatt acttttgcat ccatttgttt gctccttttt tccaaaataa
     2521 aaagccagtg ccgaagcact gactcttaac tattacttac acttgctaaa ccagaaacac
     2581 gaccaaaagc tatatcctaa aattccctta agcatggtaa tcacctcctt taaatgccaa
     2641 aaatagtttt taacaaggct ataacaaatg tacttagaat cgtccctatt aatcctagaa
     2701 tccacatctt gatgtctcta atatttttag catttttctc tttattt
//
LOCUS       DAHHOJ010000179         2674 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.182, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000179 DAHHOJ010000000
VERSION     DAHHOJ010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2674
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.182"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            142..1506
                     /gene="radA"
                     /locus_tag="NBH32_002578"
     CDS             142..1506
                     /gene="radA"
                     /locus_tag="NBH32_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCD1516141.1"
                     /translation="MAKKKVIFECMACGYQSPKWMGKCPNCGAWNQMEEIVEKAANPK
                     HGVKTKELAGKVQKLNSIKHETTPRVLTDSAEFNRVLGGGIVSGSLVLIGGDPGIGKS
                     TLLLQICASLSQKKKVLYITGEESLSQTKLRAERLDEDSSELQVLAETDLEVIYQTVK
                     EEQPDLLVVDSIQTIYHPEISSAPGSVSQVRESTQSLMNIAKQMNIATFIVGHVTKEG
                     QIAGPRLLEHMVDTVLYFEGDEHHAYRILRAVKNRFGSTNEMGIFEMKQSGLKGVNNP
                     SEMFLEERSTNVPGSTIVATMEGTRPLLIEVQALVTPTTFNNPRRMATGIDHNRLSLL
                     MAVLEKKENYLLQQQDAYIKVAGGVKLTEPAVDLSVIVATASSFKDEAVDGLDCYIGE
                     VGLTGEVRRVSRIEQRVQEAAKLGFKRVIIPKNNIGGWTYPEGIQVIGVTTVHEALSF
                     ALHS"
     gene            1531..2604
                     /locus_tag="NBH32_002579"
     CDS             1531..2604
                     /locus_tag="NBH32_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017328539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HCD1516142.1"
                     /translation="MNIVKLMVIIIYLIIGSALGIIIIPEIANDLGLQNSSFLKNHYV
                     DGIIGSIFMFLIFGVFIRRVTNAIKGLEHFIMRRSAVEILFATIGLIIGLLISVMVSF
                     ILESIGNSIFNHFIPVIITILLCYFGFQFGLKKRDEMLMFLPENIARSMSQHTKSATP
                     KIIDTSAIIDGRILEVIRCGFIDGNILIPQGVINELQIVADSNDSVKREKGKRGLDIL
                     NELYDLDYPTKVIHPTKTHSDIDTMLLKLAKQYHASIITTDFNLNKVCHVHGIKALNV
                     NDLSEAIKPNVHQGDQLHILLTKMGKEPGQAVGYLDDGTMVVVDNAKNLIGSHVNLEV
                     VSLLQTSSGRIVFAKKIEDTVSL"
BASE COUNT          944 a          367 c          533 g          830 t
ORIGIN      
        1 attcataaaa aataaatctg ataaaacagt ttgcgttagt agtcatgtta aaatacaaac
       61 aaatgtaact cacatttaat ttgtcataat gggaatgtgc gtttaaaata gatttgttca
      121 aaggaaagtg gaggtgcaat tttggccaag aaaaaagtga tttttgaatg tatggcttgt
      181 ggttatcaat ctcctaaatg gatggggaaa tgtcctaatt gtggcgcttg gaatcaaatg
      241 gaggaaattg ttgaaaaagc agccaatcct aaacatggag tgaaaaccaa ggaattagca
      301 ggtaaagtac aaaaattaaa tagtattaaa catgaaacaa cgccgagagt gttaacagat
      361 tcagcagaat tcaaccgcgt attaggtgga ggtattgtga gcggatcgtt agtacttatt
      421 ggtggggatc caggtattgg taagtcaacg ttacttttac aaatttgtgc atcgttatct
      481 caaaagaaaa aagtactata tattactgga gaagaatcgc ttagtcagac taaattacgt
      541 gcagagcgat tagatgaaga ttcaagtgaa ttgcaagtat tagctgaaac agatcttgaa
      601 gttatttatc aaacagtaaa agaagaacaa cctgatttac tagtagtgga ttcgattcaa
      661 acaatatatc atcctgaaat cagctctgcg ccaggttctg tttcacaagt tcgtgaaagt
      721 acacaaagtt taatgaatat tgctaaacaa atgaacattg caacttttat agtgggtcat
      781 gtaacgaaag aaggtcaaat tgctggccca agattgctag aacacatggt tgatactgtg
      841 ctttattttg aaggcgatga acaccacgca tatcgaattt tgcgagctgt taaaaaccgt
      901 tttggttcaa cgaatgaaat gggaatcttc gaaatgaagc aaagtggatt aaaaggtgta
      961 aataatccat ctgaaatgtt tttagaagaa cgttcaacaa atgttccagg ttcaacaatt
     1021 gttgcaacca tggagggaac cagaccactt ttaatagaag ttcaagcgct ggtaactcca
     1081 acgactttta acaatccgag acgaatggca acagggattg atcataatcg attaagtttg
     1141 ttgatggctg ttttggaaaa gaaagaaaat tatctattac aacaacaaga tgcttatatc
     1201 aaagtagctg gcggtgtaaa gttaacggag ccagcagttg atttaagtgt aattgtagca
     1261 actgcgtcta gctttaaaga tgaagctgtc gacggattag attgctatat tggagaagtt
     1321 ggtttaacgg gtgaggtacg tcgtgtatct cggatagaac aacgcgtgca agaggctgca
     1381 aaactaggtt tcaaacgtgt aattattcct aaaaataata taggcggatg gacatatcct
     1441 gaaggtatac aagtaatagg tgtaactact gtacatgaag cattgtcatt tgctcttcat
     1501 tcataaaaca tcaagaaagg aggacattgt gtgaatatcg ttaaactaat ggttattatt
     1561 atttacttaa ttattgggag cgcattagga ataattatta ttcctgaaat tgcaaatgat
     1621 cttggattac aaaactccag ctttttaaaa aatcactatg tagatggcat tatcggtagt
     1681 atttttatgt tcttaatttt tggtgtattt attagacgag ttactaacgc tataaaaggt
     1741 ttagaacatt ttattatgcg tagaagtgct gttgaaatac tatttgcaac aataggttta
     1801 ataatcggat tacttatttc tgttatggtg tcgtttatat tagaatcaat tggcaactct
     1861 atttttaatc atttcattcc tgtcataatt acgatattac tatgttattt cggtttccaa
     1921 tttggcctta aaaaacgaga tgaaatgtta atgtttttac ctgagaatat agcgcgttcc
     1981 atgtcacaac atactaaaag tgctacgcca aaaattatcg acacaagcgc aattattgat
     2041 ggtcgtattt tagaagtcat tcgttgcggt tttatcgatg gcaatatttt aattccacaa
     2101 ggtgttatta atgaattaca aattgttgca gattcaaatg acagtgttaa acgtgaaaag
     2161 ggtaaaagag gcttagatat tttaaatgaa ttgtatgatt tagattatcc tacaaaagtc
     2221 atacatccaa ctaaaacaca tagtgatatt gatacaatgt tattaaagct agctaaacaa
     2281 tatcatgcaa gtattattac gacagatttc aacctaaata aagtttgtca tgtacatggt
     2341 atcaaagcat taaatgttaa tgatttatca gaagcaatca aacctaatgt acatcaaggt
     2401 gatcaactgc atattttact gacaaaaatg ggtaaagagc ctggtcaggc agtaggatat
     2461 ctagatgatg gtacgatggt agttgttgat aatgctaaaa atcttattgg cagtcatgtc
     2521 aatttagaag tagtcagctt attgcaaaca tcttcaggaa gaattgtttt tgctaaaaaa
     2581 atcgaagata cagtatcatt ataaaaactg tagttgacga aaacaaaata gatattaaaa
     2641 ggtatatgaa atggcgaagt ttgtaatttg aagt
//
LOCUS       DAHHOJ010000180         2670 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.183, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000180 DAHHOJ010000000
VERSION     DAHHOJ010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2670)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2670)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2670
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.183"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..151)
                     /locus_tag="NBH32_002580"
     CDS             complement(<1..151)
                     /locus_tag="NBH32_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516143.1"
                     /translation="MKNIISIILGILMFLKSMELLYGAIFLDKPLNPITKIIFILTLI
                     YIFYVL"
     gene            complement(361..810)
                     /gene="ndk"
                     /locus_tag="NBH32_002581"
     CDS             complement(361..810)
                     /gene="ndk"
                     /locus_tag="NBH32_002581"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCD1516144.1"
                     /translation="MERTFLMIKPDAVQRNLIGEVISRIERKGLKLVGGKLMQVPMEL
                     AETHYGEHQGKPFYNDLISFITSAPVFAMVVEGEDAVNVSRHIIGSTNPSEASPGSIR
                     GDLGLTVGRNIIHGSDSLESAEREINLWFNENEITSYASPRDAWLYE"
     gene            complement(902..1861)
                     /locus_tag="NBH32_002582"
     CDS             complement(902..1861)
                     /locus_tag="NBH32_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002463052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HCD1516145.1"
                     /translation="MAKLNMNNEIKKVEQRLEKAIKSKDSVLEQASLHLLSSGGKRVR
                     PAFVILSSQFGKDEQTSEQTYQVAVALELIHMATLVHDDVIDKSDKRRGKLTISKKWD
                     QTTAILTGNFLLALGLEHLMAVKDNRVHQLISESIVDVCRGELFQFQDQFNSQQTIIN
                     YLRRINRKTALLIQLSTEVGAITSQSDKETVRKLKMIGHYIGMSFQIIDDVLDFTSTE
                     KKLGKPVGSDLLNGHITLPILLEMRKNPEFKVKIEQLRRDSERKEFEECIQIIRKSDS
                     IDEAKAVSSKYLSKALDLISELPDGHPKSLLLSLTKKMGSRNT"
     gene            complement(1863..2588)
                     /locus_tag="NBH32_002583"
     CDS             complement(1863..2588)
                     /locus_tag="NBH32_002583"
                     /EC_number="2.1.1.163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="demethylmenaquinone methyltransferase"
                     /protein_id="HCD1516146.1"
                     /translation="MADNKANKEQVHRVFQNISKKYDKLNNIISFEQHKVWRKRVMKD
                     MGVRKGTKALDVCCGTGDWTIALSKAVGPTGEVTGIDFSENMLEVGKEKTASMENVKL
                     VHGDAMELPFEDNSFDYVTIGFGLRNVPDYLVALKEMNRVLKPGGMVVCLETSQPTLP
                     VFKQMYALYFKFVMPIFGKLFAKSKEEYEWLQQSTFNFPGKEELKRMFEEAGFINVRV
                     RSFTGGVAAMHLGYKEKDNTKGD"
     gene            complement(2591..>2670)
                     /locus_tag="NBH32_002584"
     CDS             complement(2591..>2670)
                     /locus_tag="NBH32_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heptaprenyl pyrophosphate synthase subunit A"
                     /protein_id="HCD1516147.1"
                     /translation="KKYNQSEVKHYLHDIQKSYLKSRGN"
BASE COUNT          821 a          518 c          343 g          988 t
ORIGIN      
        1 ctaatacata aaaaatataa atgagagtca gtataaaaat aatttttgtt ataggattaa
       61 gtggtttatc taaaaatata gcaccatata ataattccat tgattttaag aacattaaaa
      121 tccctaaaat aattgaaata atattcttca ttctatcaac cccttctttt agtttagttt
      181 catttgataa agaatattcc tctcttttta atacgtgtta ccaaaaaaac tataaaattt
      241 ttcaagtaat ttactacaac ataaaaaacg ggccttgtta tgtataatca taatgactaa
      301 ccacataaca aaaccctttc tacctttata aataaatcgt aaaggtttac agtttaaatt
      361 ttattcatat aaccatgcat cacgtggtga agcatagcta gtaatttcat tttcattaaa
      421 ccatagatta atttcacgtt cagcagactc taatgaatct gaaccgtgaa tgatatttct
      481 accaacagtt aaacctaaat cacctctaat tgatcctggt gaagcttctg aaggatttgt
      541 gctgccaata atatgtctag atacattaac tgcatcttca ccttcaacta ccattgcgaa
      601 cactggtgct gatgtaataa atgaaattaa atcattataa aatggtttac cttggtgttc
      661 accataatgt gtttcagcaa gttccattgg tacttgcatt aatttaccac cgacaagttt
      721 tagtcctttt ctttcaattc ttgaaattac ttcaccaatt agatttcttt gtactgcatc
      781 tggtttaatc attaaaaatg tacgttccac tttatgtatc cccctgtgtt taataggttt
      841 atgtatatta ctattatata ttaacaggtt ggtaaagcgc tttcaatact tttcataaat
      901 actacgtgtt tcttgaaccc atttttttcg tcaaacttaa aagtagtgat ttcggatgtc
      961 catctggtaa ctcagaaatc aaatccaaag ctttacttaa atacttcgaa cttactgcct
     1021 tagcctcatc gatgctgtca gattttctaa tgatttggat acattcttca aattctttac
     1081 gttcactatc acgtcgtaat tgctcaattt tcactttgaa ttctggattt tttctcattt
     1141 ccaataaaat aggcaaagta atatgaccat tcaacaaatc acttccgacc ggcttaccta
     1201 atttcttttc ggtacttgtg aagtctaata catcatcaat gatttggaag ctcataccta
     1261 tataatgacc aatcattttc aattttcgta cagtctcttt atcagattga gatgtaattg
     1321 caccaacttc agttgataat tgaattaaca gtgctgtttt gcgattgata cgtcgtaagt
     1381 aattgataat tgtctgttga ctgttaaatt ggtcttgaaa ttggaaaagt tcccctctac
     1441 aaacatcaac tatagattca gatattaatt gatgtacacg attatcttta acggccatta
     1501 agtgttcaag tcctaatgcc aataaaaaat tcccagttaa aatagcagtt gtctgatccc
     1561 atttctttga tatggttaac ttgcctcgac gcttgtcgct tttatcaata acgtcatcat
     1621 gaacaagtgt tgccatatga attaactcta atgcgactgc aacttgatac gtttgttcag
     1681 acgtttgttc atctttgcca aattggctac tcagaattac aaatgctggg cgtactcttt
     1741 taccaccaga agataataag tgtaatgatg cctgttctaa tacagaatct ttacttttta
     1801 ttgccttttc aagtcgttgt tccactttct taatttcatt gttcatgttt aactttgcca
     1861 cgttaatcac ctttggtatt atctttttct ttatagccaa ggtgcattgc agcaacgccc
     1921 cctgtaaaac tacgtactct tacatttatg aaacccgctt cttcaaacat gcgcttcaac
     1981 tcttcttttc caggaaaatt aaacgtagat tgctgtaacc attcatattc ttcttttgat
     2041 tttgcaaata atttcccaaa aataggcata acaaatttaa agtataatgc atacatttgt
     2101 ttaaagactg gcaaagttgg ttggctcgtt tcaagacata ccaccatacc acctggttta
     2161 agtactctat tcatttcttt taacgcgact aaatagtctg gcacattcct taatccaaac
     2221 ccaattgtta cataatcaaa agaattgtct tcaaacggca attccattgc atcaccatga
     2281 acaagtttaa cattttccat tgaagcagtt ttttcttttc ctacttctaa catattctca
     2341 ctaaagtcaa taccagtaac ttcacctgtt ggtcctacag ctttgcttaa tgcgattgtc
     2401 caatcaccag taccacaaca aacatctaat gctttcgtcc cttttctaac acccatgtct
     2461 ttcatgacgc gttttctcca tactttatgc tgctcaaaac taataatatt atttaatttg
     2521 tcatattttt ttgaaatatt ttgaaaaacg cgatgtactt gctctttatt tgctttattg
     2581 tcagccatgc ttaattacct ctacttttta aataactttt ttggatatcg tgtaagtaat
     2641 gctttacttc actttgatta tatttcttga
//
LOCUS       DAHHOJ010000181         2638 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.184, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000181 DAHHOJ010000000
VERSION     DAHHOJ010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2638
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.184"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..1218
                     /locus_tag="NBH32_002585"
     CDS             <1..1218
                     /locus_tag="NBH32_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516148.1"
                     /translation="HIRNQLFDMTIYRTYLVMNYGTVDEKEIKAKGKDRIDNILKLDY
                     DKKSEKELDDIVKDEVEKKNNKYMTQGYVFQKLAISVIGFIITLFMSIVFLSISFAKL
                     IFSTFALFLFLFLVFSWIVSFIPGFELSVFSAFAKTLGYIILSACMTFLFVIVGLCIK
                     LANSFIEPDSQNAYFLNSIFIIVILFVMYKKRAQIINFVSRGNISFSPSAIGAGVMNR
                     TQERFNKIRHEQAQNKKAKRENQRDEPAPPLQNDNDLRRRQQDKPMPLFINKDNQKNG
                     NKRREQQESMNGNDVKSASVESNANNYSKQPQKASQQEHQVRETRQRKDIQRSPQVVN
                     QPLNNENHSINRKEQKSVQTAYDTDVQKRQIQNATQNQQSRQSGNRNQPITRNYQSKD
                     RLKEQKDINKHGK"
     gene            1208..2254
                     /locus_tag="NBH32_002586"
     CDS             1208..2254
                     /locus_tag="NBH32_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000247473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HCD1516149.1"
                     /translation="MVNKKDIAKKVIGKTPIGVKLKLAKVIIILCVVAFFIFPVIIMF
                     LLAPDLNKGKDDAGCTVSGGNVSANGIDKFNENAKGGKLEGKGKEIQKIAEKNKVPVN
                     IFMAIIASESQWGKGENATRQNNPLSVMGSKSIHDSTYPTIEDGLNAGAKNLYDVYIS
                     KGLDTPKKIGPKYAPVGASNDPNNMNARWIPTVEKIMKDLGGSEAKTSCSNGKGKSIK
                     FNGKLPHWSNDDPGKGNLYTAGQCTWYAYGMRQKMGKPVSTYWHDAHKWNDRAKAEGY
                     KVDKNPEPGALFIAEQGAGGHDGHYGHVAVVIGVSDGGKTFRITEMNWEGAFKVNERT
                     LKMTDGYSFIHDKE"
     gene            2261..>2638
                     /locus_tag="NBH32_002587"
     CDS             2261..>2638
                     /locus_tag="NBH32_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516150.1"
                     /translation="MKQKIIMFTLVTIILFCAVLIGYQIPKQQVKMKQNQIEDLQEEQ
                     RILRDKNGELNKLVKRQSKTVISDEEKQIREVSSNFVKQMFEMKKDSSFKSKAPQIKP
                     LVTKDYYDTLFKDSKDKYDLYDDI"
BASE COUNT         1019 a          314 c          494 g          811 t
ORIGIN      
        1 catatacgta atcaattatt tgatatgacg atatatagaa catatttagt aatgaattat
       61 ggaactgtcg acgaaaaaga gataaaagca aaaggtaaag accgtattga taacatttta
      121 aaacttgatt atgataaaaa atcagaaaaa gaattagatg atattgttaa agatgaggtt
      181 gaaaagaaaa ataataaata tatgacgcaa ggttatgtat ttcaaaagtt ggctatttca
      241 gtgattggtt ttattattac actttttatg tcgatagtat ttttatcaat aagttttgcg
      301 aagttgattt tttctacatt tgctttattt ttattcttat ttttagtatt cagttggata
      361 gtgagtttta taccaggatt tgaattatct gttttctctg cgtttgcaaa aacgttaggc
      421 tatattattt taagtgcttg tatgacattc ttatttgtga tagttggttt gtgtatcaag
      481 cttgcaaata gttttattga accggatagt caaaatgctt attttttaaa ttcaatattt
      541 ataattgtaa tattgtttgt aatgtataaa aaacgggctc aaattatcaa ttttgtaagt
      601 cgtggtaata ttagtttttc tcctagtgca attggtgctg gtgtgatgaa tcgaacacaa
      661 gaacgtttta ataagattcg tcatgaacaa gcgcaaaata agaaagctaa acgagaaaat
      721 caaagagatg aacccgcccc accacttcaa aatgataatg atttaagaag aagacaacag
      781 gataaaccaa tgcctttatt tataaataaa gataatcaaa aaaatggtaa taagcgcaga
      841 gaacaacaag agtcaatgaa tggtaacgat gtaaaatctg cttctgttga atcaaatgca
      901 aataattatt ctaaacaacc acaaaaagca tcacagcaag aacatcaagt acgagaaaca
      961 agacagcgaa aagatataca aaggtcgcca caagtggtta atcagccatt aaataatgag
     1021 aatcattcaa taaatcgtaa agaacagaag tcagttcaaa ctgcttatga tactgatgtt
     1081 caaaaacgac aaatacaaaa tgcgacacag aatcaacaaa gtagacaatc tggaaatcgt
     1141 aatcaaccta ttacgcgtaa ttatcagtca aaagaccgtt taaaagagca aaaggatatc
     1201 aataagcatg gtaaataaaa aagatattgc taaaaaagta ataggtaaaa cgccaatagg
     1261 cgttaaattg aaattagcga aagtcatcat cattttatgt gtggtggctt tttttatatt
     1321 tccagtaatt ataatgtttt tgttagcgcc tgatttaaat aaaggtaaag atgatgctgg
     1381 ttgtactgtt agtggcggta atgtttcggc aaatggtatt gataagttta acgaaaatgc
     1441 taaaggtgga aaattagagg gtaaaggaaa agaaatacag aaaattgcag aaaaaaataa
     1501 agtacctgtg aatattttta tggccattat tgcgagtgaa tcacaatggg gtaaaggaga
     1561 aaatgcgaca agacaaaata atcctttgtc tgttatgggt tcaaaatcga ttcatgattc
     1621 aacatatcca actattgaag atggtcttaa tgcgggagct aaaaatttgt atgatgttta
     1681 tatttctaaa ggtttagata ctcctaaaaa aataggtccc aaatatgcgc ctgttggtgc
     1741 gtcaaatgac cctaataata tgaatgcccg ttggatacca acagttgaaa aaattatgaa
     1801 agatttaggc ggttcagaag ctaaaacgtc atgtagtaat ggtaaaggaa agtctataaa
     1861 gtttaatggt aaattgccac attggtcaaa tgatgaccct ggaaaaggta atttatacac
     1921 tgctggtcaa tgtacttggt atgcatacgg tatgcgtcaa aaaatgggta aacctgtatc
     1981 gacttattgg catgatgcac ataaatggaa tgatagagct aaagctgagg gttataaagt
     2041 tgataaaaat ccggaacctg gtgcattatt tattgctgaa caaggtgcag gcggtcatga
     2101 tgggcattat ggtcatgtgg ctgttgttat tggtgtaagt gatggtggta aaacatttag
     2161 aataacagaa atgaattggg aaggtgcttt taaagttaat gaacgtacat tgaaaatgac
     2221 tgacggatat agttttattc atgataagga gtgaaaataa atgaagcaaa aaattattat
     2281 gtttactctt gtaacaataa tattattttg tgctgtttta attggttatc agattcctaa
     2341 acaacaagtt aaaatgaaac aaaatcaaat tgaagatttg caggaagaac aacgtatatt
     2401 aagagataaa aatggagagt taaataaatt agtaaagaga caatcaaaaa cagttatttc
     2461 tgatgaagaa aaacaaatac gtgaagtttc ttcaaatttt gttaaacaga tgtttgaaat
     2521 gaaaaaagat tcttctttta aatcaaaagc accacaaata aagcctttag ttacaaagga
     2581 ttattacgat acattattta aggattctaa agataaatat gatttatatg atgatata
//
LOCUS       DAHHOJ010000182         2633 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.185, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000182 DAHHOJ010000000
VERSION     DAHHOJ010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2633
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.185"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(63..146)
                     /locus_tag="NBH32_002588"
     tRNA            complement(63..146)
                     /locus_tag="NBH32_002588"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(110..112),aa:Leu,seq:caa)
     gene            complement(185..259)
                     /locus_tag="NBH32_002589"
     tRNA            complement(185..259)
                     /locus_tag="NBH32_002589"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(224..226),aa:Gly,seq:tcc)
     gene            complement(363..437)
                     /locus_tag="NBH32_002590"
     tRNA            complement(363..437)
                     /locus_tag="NBH32_002590"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(402..404),aa:Gly,seq:tcc)
     gene            complement(445..518)
                     /locus_tag="NBH32_002591"
     tRNA            complement(445..518)
                     /locus_tag="NBH32_002591"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(484..486),aa:Cys,seq:gca)
     gene            complement(524..595)
                     /locus_tag="NBH32_002592"
     tRNA            complement(524..595)
                     /locus_tag="NBH32_002592"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(561..563),aa:Gln,seq:ttg)
     gene            complement(607..679)
                     /locus_tag="NBH32_002593"
     tRNA            complement(607..679)
                     /locus_tag="NBH32_002593"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(644..646),aa:His,seq:gtg)
     gene            complement(682..755)
                     /locus_tag="NBH32_002594"
     tRNA            complement(682..755)
                     /locus_tag="NBH32_002594"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(721..723),aa:Trp,seq:cca)
     gene            complement(771..851)
                     /locus_tag="NBH32_002595"
     tRNA            complement(771..851)
                     /locus_tag="NBH32_002595"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(815..817),aa:Tyr,seq:gta)
     gene            complement(857..932)
                     /locus_tag="NBH32_002596"
     tRNA            complement(857..932)
                     /locus_tag="NBH32_002596"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(897..899),aa:Thr,seq:tgt)
     gene            complement(940..1012)
                     /locus_tag="NBH32_002597"
     tRNA            complement(940..1012)
                     /locus_tag="NBH32_002597"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(977..979),aa:Phe,seq:gaa)
     gene            complement(1031..1106)
                     /locus_tag="NBH32_002598"
     tRNA            complement(1031..1106)
                     /locus_tag="NBH32_002598"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1071..1073),aa:Asp,seq:gtc)
     gene            complement(1116..1189)
                     /locus_tag="NBH32_002599"
     tRNA            complement(1116..1189)
                     /locus_tag="NBH32_002599"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1153..1155),aa:Met,seq:cat)
     gene            complement(1204..1296)
                     /locus_tag="NBH32_002600"
     tRNA            complement(1204..1296)
                     /locus_tag="NBH32_002600"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1258..1260),aa:Ser,seq:tga)
     gene            complement(1335..1410)
                     /locus_tag="NBH32_002601"
     tRNA            complement(1335..1410)
                     /locus_tag="NBH32_002601"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1375..1377),aa:Asp,seq:gtc)
     gene            complement(1421..1510)
                     /locus_tag="NBH32_002602"
     tRNA            complement(1421..1510)
                     /locus_tag="NBH32_002602"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1472..1474),aa:Ser,seq:tga)
     gene            complement(1520..1593)
                     /locus_tag="NBH32_002603"
     tRNA            complement(1520..1593)
                     /locus_tag="NBH32_002603"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1557..1559),aa:Ile,seq:cat)
     gene            complement(1622..1695)
                     /locus_tag="NBH32_002604"
     tRNA            complement(1622..1695)
                     /locus_tag="NBH32_002604"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1659..1661),aa:Met,seq:cat)
     gene            complement(1717..1792)
                     /locus_tag="NBH32_002605"
     tRNA            complement(1717..1792)
                     /locus_tag="NBH32_002605"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1757..1759),aa:Ala,seq:tgc)
     gene            complement(1810..1883)
                     /locus_tag="NBH32_002606"
     tRNA            complement(1810..1883)
                     /locus_tag="NBH32_002606"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1847..1849),aa:Pro,seq:tgg)
     gene            complement(1893..1966)
                     /locus_tag="NBH32_002607"
     tRNA            complement(1893..1966)
                     /locus_tag="NBH32_002607"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1930..1932),aa:Arg,seq:acg)
     gene            complement(1985..2073)
                     /locus_tag="NBH32_002608"
     tRNA            complement(1985..2073)
                     /locus_tag="NBH32_002608"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2037..2039),aa:Leu,seq:taa)
     gene            complement(2084..2158)
                     /locus_tag="NBH32_002609"
     tRNA            complement(2084..2158)
                     /locus_tag="NBH32_002609"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2124..2126),aa:Gly,seq:gcc)
     gene            complement(2162..2243)
                     /locus_tag="NBH32_002610"
     tRNA            complement(2162..2243)
                     /locus_tag="NBH32_002610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2207..2209),aa:Leu,seq:tag)
     gene            complement(2247..2322)
                     /locus_tag="NBH32_002611"
     tRNA            complement(2247..2322)
                     /locus_tag="NBH32_002611"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2287..2289),aa:Lys,seq:ttt)
     gene            complement(2328..2403)
                     /locus_tag="NBH32_002612"
     tRNA            complement(2328..2403)
                     /locus_tag="NBH32_002612"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2368..2370),aa:Thr,seq:tgt)
     gene            complement(2420..2495)
                     /locus_tag="NBH32_002613"
     tRNA            complement(2420..2495)
                     /locus_tag="NBH32_002613"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2460..2462),aa:Val,seq:tac)
     gene            complement(2507..2621)
                     /gene="rrf"
                     /locus_tag="NBH32_002614"
     rRNA            complement(2507..2621)
                     /gene="rrf"
                     /locus_tag="NBH32_002614"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          727 a          679 c          624 g          603 t
ORIGIN      
        1 aaaaagaaac cttgttttac aaggtttcta atacgttatg ttatgtaaat aacagttaat
       61 tataccggtg gtcggggtcg aaccgacact ccacaagtgg aacgggattt tgagtcccgc
      121 gcgtctgcca attccgccac accggcttaa tggtaaacaa aaaacttccc tttggaagca
      181 attatggagc ggaagatagg atttacacct atacctcgtt ccgggaagga acgtgttcta
      241 aaagttgaac tactcccgca aatattaaat tatggagcgg aagataggat ttacacctat
      301 acctcattcc aggaaggaat gtattctaag agttgaaata ctcccgcatt attattaaat
      361 tatggagcgg aagataggat ttgcacctat acctcgttcc gggaaggaac gtgttctaaa
      421 agttgaacta ctcccgcata aacctggagg cggcaaccgg atttgaaccg gtgataaagg
      481 ttttgcagac ctctgcctta ccacttggct atgccgccaa taactgggct agctggattc
      541 gaaccaacga gtgacggagt caaagtccgt tgccttaccg cttggctata gcccattaat
      601 aataagggcg gctgaagggg atcgaaccct cgaatgtcgg aaccacaatc cgatgtgtta
      661 accacttcac cacagccgcc atggcagggg cagtaggaat cgaacccaca ccaaaggttt
      721 tggagacctc tattctaccg ttgaactatg cccctattaa aaataataaa tggagggggg
      781 cagattcgaa ctgccgaacc cgaaggagcg gatttacagt ccgccgcgtt tagccacttc
      841 gctacccctc cataaatggt gccggccaga ggacttgaac ccccaaccta ctgattacaa
      901 gtcagttgct ctaccaattg agctaggccg gctaagaaat ggttcaggac agagtcgaac
      961 tgccgacaca tggagcttca atccattgct ctaccaactg agctactgaa ccataataaa
     1021 aatgtaatga tggcggtctc gacgggaatc gaacccgcga tctcctgcgt gacaggcagg
     1081 cgtgttaacc gctacactac gagacctata aaatattgcg ggaggcggat ttgaaccacc
     1141 gaccttcggg ttatgagccc gacgagctac cgaactgctc catcccgcga taataaaaaa
     1201 taatggcgga ggaagaggga ttcgaacccc cgcggcccgt taaggccctg tcggttttca
     1261 agaccgatcc cttcagccgg acttgggtat tcctccatta ttataggtaa atcgctatta
     1321 attataaaat taaatggcgg tctcgacggg aatcgaaccc gcgatctcct gcgtgacagg
     1381 caggcgtgtt aaccgctaca ctacgagacc attagtaaaa cggaggaaga gggattcgaa
     1441 cccccgcgag ccgttaagcc cctgtcggtt ttcaagaccg atcccttcag ccggacttgg
     1501 gtattcctcc aaaattatat ggaccttgca ggactcgaac ctgcgaccga acggttatga
     1561 gccgttagct ctaaccaact gagctaaagg tcctaaatat aattttacaa ctaataaata
     1621 gtggcggtgg aggggatcga acccccgacc tcacgggtat gaaccgtacg ctctagccag
     1681 ctgagctaca ccgccttata tagtttgtaa ataatatggt ggagactagc gggatcgaac
     1741 cgctgacctc ctgcgtgcaa agcaggcgct ctcccagctg agctaagccc ccataataat
     1801 tacagtatat cgggaagaca ggattcgaac ctgcgacccc ttggtcccaa accaagtgct
     1861 ctaccaagct gagctacttc ccgtataatt aacgcgcccg ataggagtcg aacccataac
     1921 ctcttgatcc gtagtcaaac gctctatcca attgagctac gggcgcatat gtttttattg
     1981 aaaatggtgc cgaggaccgg aatcgaaccg gtacggtgat cactcaccgc aggattttaa
     2041 gtcctgtgcg tctgccagtt ccgccacccc ggcactataa aaatggagca gaagacggga
     2101 ttcgaacccg cgaccccaac cttggcaagg ttgtattcta ccgctgaact acttctgcat
     2161 atgcgggtga agggagtcga acccccacgc cgtaaggcgc tagatcctaa gtctagtgcg
     2221 tctgccaatt ccgccacacc cgcaaatggt gagccataga ggattcgaac ctctgaccct
     2281 ctgattaaaa gtcagatgct ctaccaactg agctaatggc tcttccatgg tgccggccag
     2341 aggacttgaa cccccaacct actgattaca agtcagttgc tctaccaatt gagctaggcc
     2401 ggcaatatgt aagaataaat ggtggagaat gacgggttcg aaccgccgac cctctgcttg
     2461 taaggcagat gctctcccag ctgagctaat tctccgattt aaaactgcct ggcaacgttc
     2521 tactctagcg gaacgtaagt tcgactacca tcgacgctaa ggagcttaac ttctgtgttc
     2581 ggcatgggaa caggtgtgac ctccttgcta tagtcaccag acatatgaat gta
//
LOCUS       DAHHOJ010000183         2621 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.186, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000183 DAHHOJ010000000
VERSION     DAHHOJ010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.186"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            119..610
                     /locus_tag="NBH32_002615"
     CDS             119..610
                     /locus_tag="NBH32_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HCD1516151.1"
                     /translation="MDWILPIAGIIAAIAFLILCIGIVAVLNSVKKNLDYVAKTLDGV
                     EGQVQGITRETTDLLHKVNRLTEDIQGKVDRLNSVVDAVKGIGDSVQTLNSSVDRVTN
                     SITHNISQNEDKISQVVQWSNVAMEIADKWQNRHYRRGSANYKANNVATDANHSYTSR
                     VDK"
     gene            684..2042
                     /locus_tag="NBH32_002616"
     CDS             684..2042
                     /locus_tag="NBH32_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516152.1"
                     /translation="MTQQQNNKRTLKNKHTYHNELLPNRKDFVVSFITGALVGSALGL
                     YFKNKVYQKADDLKVKEQELSQKFEERKTQLEETVAFTKERVEGFLNKSKNEQAALKA
                     QQAAIKEEASANNLSDTSQEAQEIQEAKKEAQAETDKSAEVSNKESKAVALKAQQAAI
                     KEEASVNNLSDTSQEAQEIQEAKKEAQAETDKSAAVSNEESKAVALKAQQAAIKEEAS
                     ANNLSDTSQEAQEIQEAKKEAQAETDKSEAVSNEESKAVALKVSKEESQAERLANAAK
                     QKQAKLTPGSKESQLTEALFAEKPVAKNDLKEIPQLITKKNDVSETVNTDNKDTVKQK
                     EAKFENGVITRKADEKTTNNTDVDKKSGKQSKKTTPSNKRNASKASTNKTSGQKKQHN
                     KKSSQGAKKQSSSSKSTQKNNQTSNKNSKATNAKSSNASKKSNAKVEKAKSKIEKRTF
                     ND"
     gene            2198..2281
                     /locus_tag="NBH32_002617"
     CDS             2198..2281
                     /locus_tag="NBH32_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516153.1"
                     /translation="MLFVTSLDYNSDIAHFKLKMEKYSIIK"
BASE COUNT         1141 a          346 c          442 g          692 t
ORIGIN      
        1 caaaatgcat atttagataa attaggcatg aaaaatgcgt tttaatatgt ttataataga
       61 gtagtatggg tatttattat taatgacatt attacatgtt aattaggagg cgtttttaat
      121 ggattggatt ttaccaattg ctggaattat cgctgcgatt gcattcttaa ttttatgtat
      181 cggtatcgta gctgtattaa attctgttaa gaaaaactta gattatgttg caaaaacact
      241 tgacggtgta gaaggtcaag ttcaaggtat tactcgtgaa acaacagatt tacttcataa
      301 agtaaaccgt ttaactgagg atatccaagg taaagtagat cgtttaaact cagttgtaga
      361 tgctgttaaa ggtatcggtg actcagtaca aacgttaaac agctctgtag atcgtgtaac
      421 aaattcaatt acacataata tttctcaaaa tgaagataaa atctcacaag ttgttcaatg
      481 gtcaaatgtt gcaatggaaa ttgcagacaa atggcaaaat agacactacc gtcgtggaag
      541 tgcaaattac aaagctaata atgtagcaac tgatgcaaat catagctata cttctcgagt
      601 agataaataa ttaataatta aacaaatgca cttaaatgag gttgttactt atttaagtgc
      661 atttgttata ggaggatata caaatgacgc agcaacaaaa taataaaaga acattaaaaa
      721 ataaacacac ttatcataat gaactactac cgaaccgtaa agattttgtt gttagtttta
      781 taactggcgc gcttgttggt tcagctttag gcttgtattt taaaaataaa gtttatcaaa
      841 aagcagatga tttaaaagtc aaagaacaag aactgtcgca aaagtttgaa gaaagaaaaa
      901 cgcaacttga agaaacggtt gcctttacaa aagaacgtgt tgaaggattt ttaaacaaat
      961 ctaaaaatga gcaagcggca ttaaaagcac aacaagcagc aataaaagaa gaagcaagtg
     1021 caaataattt aagtgataca tcacaagagg cacaagagat tcaagaagct aaaaaagaag
     1081 cacaagcaga aacggataaa agtgcagaag tatcaaataa agaatcaaaa gcagtggcat
     1141 taaaagcaca acaagcagca ataaaagaag aagcaagtgt aaataattta agtgatacat
     1201 cacaagaggc acaagagatt caagaagcta aaaaagaagc acaagcagaa acagataaaa
     1261 gtgcagctgt atcaaatgaa gaatcaaaag cagtggcatt aaaagcacaa caagcagcaa
     1321 taaaagaaga agcaagtgca aataatttga gtgatacatc acaagaggca caagaaattc
     1381 aagaagctaa aaaagaagca caagcagaaa cagataaaag tgaagctgta tcaaatgaag
     1441 aatcaaaagc agtggcatta aaggtatcga aagaagagtc acaagctgaa agattagcaa
     1501 acgctgcaaa acagaagcaa gctaaattaa caccaggttc aaaagagagt caattaactg
     1561 aagcgttatt tgcagaaaaa ccagttgcta aaaatgactt gaaagaaatt cctcaattaa
     1621 ttactaaaaa gaatgatgta tcagaaacag ttaatacaga taataaagac actgtaaaac
     1681 aaaaagaagc taaatttgaa aatggtgtta ttacacgtaa agctgatgaa aaaacaacta
     1741 ataatacaga tgttgacaag aaatcaggta aacaatctaa aaaaacaaca ccttcaaata
     1801 aacgaaatgc atcaaaagca tctacaaata aaacttcagg tcagaaaaag caacataata
     1861 agaaatcatc acaaggtgct aagaaacaaa gtagttcaag taagtcaact caaaagaata
     1921 atcaaactag taataagaat tcaaaagcaa caaatgctaa atcatcaaat gcatcaaaaa
     1981 aatcaaatgc aaaagttgaa aaagctaaaa gtaaaataga gaagcgtaca tttaatgact
     2041 aagcatacta aaatcgttta ataatcattc atagcagtaa gctatgtatt atatttataa
     2101 aaattgaatt tcaaaataaa aggtaggcat tcaggtgctt accttttttg atatcatcag
     2161 tttattttga aaggcaacgc gatcattcca tgtatttatt ttgtttgtaa catcgttaga
     2221 ttacaatagt gatattgcac attttaagct gaagatggaa aaatattcga taatcaaata
     2281 aacaatgaat cttagtaatt acaatgacgt tcactaattc aaatatagct gaatatgttg
     2341 gtgagtgatg ttcactatag atttatatta atatacaaag gttgttaaat tttattaagc
     2401 gttaggttga atgtatgaga attttagatt tataatagaa gatagaaacc aaaatttttc
     2461 ttaaaagcag taatgttgac tcaaaataag ctataatcat gacacttatt taattgatta
     2521 acatgtgcta ataaatatca atatagaata taactttcaa acaatgactg agaaaatcga
     2581 aatgtcagtc aagcatcata tagttagaaa attaattatt t
//
LOCUS       DAHHOJ010000184         2557 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.187, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000184 DAHHOJ010000000
VERSION     DAHHOJ010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2557)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2557)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2557
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.187"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(84..719)
                     /locus_tag="NBH32_002618"
     CDS             complement(84..719)
                     /locus_tag="NBH32_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001168910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HCD1516154.1"
                     /translation="MQKFKDLFYDDLSVARGNYFLTLLYAFLITIILLIGFVVSDTHI
                     LYGMFIVLIGIVLLRLFKINLLSFKKLTMSQVIYIIGGALLIYGLDHLYLHFHDVPTN
                     EQELDRELQHTPLYMSIVTIAIIPAIVEEIVFRGMIIRVVFRKHLFIGLVVSSLVFAS
                     LHESDTWIGYLPYLYSGVIFGLIYLKTKRLEVVILIHFINNLSSLLILIWG"
     gene            1017..1460
                     /locus_tag="NBH32_002619"
     CDS             1017..1460
                     /locus_tag="NBH32_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001030476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516155.1"
                     /translation="MNKASFDKKVKKQLWFLNKKEKHALDQRLSSITDKDNVNFNKPI
                     TFANAYLRENVFRSKETKSYSIFVTLVVMMFAYVALLGLFLFGLITSLSGVQFFVNPK
                     VELSTTVVILTIIGAILLMLVSIYLIKITTSYFTKKLIEHKFNGH"
     gene            complement(1447..1890)
                     /locus_tag="NBH32_002620"
     CDS             complement(1447..1890)
                     /locus_tag="NBH32_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001152695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComK"
                     /protein_id="HCD1516156.1"
                     /translation="MQDISTKHLLYIQTATSNHLETKCVFLYHEYNLEFPITKAIPYY
                     AKLHFTSASILIDAAKSILNINKLVPIYLNPKTILFPIKQKRAPIQTYINAHYVIGMT
                     EMGNSTMLHFKNGKTLEVDEPMSLISKKCHESLALKHFIENTNDH"
     gene            complement(2003..2473)
                     /locus_tag="NBH32_002621"
     CDS             complement(2003..2473)
                     /locus_tag="NBH32_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000671059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCD1516157.1"
                     /translation="MKFNDVYNKHHKIIHHLLKKYNISYNYDEYYQLLLIKMWQLSQI
                     YKPSSKQSLSSFLFMRLNFYLIDLFRQQKRLNDIILYENHSPILTEQPIYFNEFNLNL
                     QDVCALLNHRERQWLMLYLEGYKQYEIADIMSLSTSTIKLIKASVKRKCQRFFK"
BASE COUNT          909 a          339 c          420 g          889 t
ORIGIN      
        1 cttgcatatc attaatttgt attgcatatc gttttgtgaa ttacatgttg aggggcaatc
       61 attgtgtatt atatcaacta gtattatccc catatcagaa tgagtaatga ggataggtta
      121 tttataaagt ggattaaaat aacgacttct aatcgctttg tttttaaata tattaggcca
      181 aaaatcactc cagaatataa ataaggtaag tacccaatcc aagtatccga ttcatgcaat
      241 gatgcaaaca ctagactaga cacaactaat ccaataaata aatgctttct aaatacaact
      301 cttattatca tgcctcgaaa aacgatttct tctacaatgg cagggataat agcaattgtg
      361 acaatagaca tatacaaagg cgtatgttgt aattctcgat ctaattcttg ttcatttgta
      421 ggtacgtcat gaaaatgtaa ataaagatga tctaacccat aaattagcaa tgcaccaccg
      481 atgatataaa tcacttgtga cattgttaat tttttaaatg agagtaaatt aattttgaat
      541 aaccgtaata acacgatacc gatgagcact ataaacatgc cataaagtat gtgtgtatca
      601 ctgacaacga agccaattag tagaataata gtaatcaaga atgcgtataa taaagttaga
      661 aaataatttc cacgtgcaac tgataaatca tcataaaaca aatccttgaa tttctgcata
      721 tttaatcctt tctatcatct ataaaatgaa tattaaagca tttcattggt atgttcattt
      781 tcattatatg agaaacttag atatgattaa aggcgcatca ttacacttaa ctaaaaacat
      841 ttgtttgcaa tataaatggt gtcaattata cagtgtcaaa ttactaaaaa taaaattgca
      901 aagttaattt ttgtagattt gatgcatgta tttctgtaaa atatataaaa tacaatgttc
      961 attggcaatc atgtataaaa tagcgtcaat atgaaaaagg agagaaatcc ttaattatga
     1021 ataaagcatc gttcgataaa aaggttaaaa agcaattatg gtttttgaac aaaaaggaaa
     1081 aacatgcatt agatcaacgg ttgtctagta ttactgacaa ggacaatgtg aactttaata
     1141 agccaatcac ctttgctaat gcgtacttaa gagaaaatgt atttcgtagt aaagaaacta
     1201 aaagttatag catctttgta acattagtag tgatgatgtt tgcatacgtt gctttattag
     1261 gtttgttttt attcggatta attacaagtc tatctggtgt gcaatttttc gttaatccta
     1321 aagtagaatt gtcgacaacg gttgttattt taacaatcat tggggcgatt ttattaatgt
     1381 tagttagtat ctatttaata aaaatcacaa catcatattt tacaaagaaa ctgatagaac
     1441 ataaatttaa tggtcattag tattttcaat gaaatgcttc aatgctaaac tttcatggca
     1501 tttttttgaa ataagagaca tgggttcatc gacttctaat gttttgccat ttttgaaatg
     1561 gagcatagtt gaattgccca tttccgtcat accaattaca tagtgtgcat tgatatatgt
     1621 ttgtatcggt gcacgctttt gttttattgg gaaaagaatc gttttcggat taaggtaaat
     1681 aggcaccagc ttgttaatgt tgagtatgct ttttgctgca tcgattaaaa ttgatgctga
     1741 tgtaaaatgc aatttagcat aataaggaat ggctttagta atagggaatt cgagattgta
     1801 ctcatggtac agaaagacac attttgtctc aagatggttc gaagttgcgg tttggatata
     1861 aagtaggtgt tttgtagaaa tgtcttgcaa atatagtact ccttttcaat cagttaataa
     1921 taaaatggtt cgcaaaaatg aaatgcatat tattgagcgt tccaagagta ttaatcattt
     1981 taatcaacac gacacgtcgc atctatttaa aaaaacgttg gcatttacgc ttaactgatg
     2041 ccttaattaa tttaattgtt gaagttgata atgacataat atcagcaatt tcatattgct
     2101 tatagccttc aagataaagc ataagccact gtcgttccct gtggtttagt agggcacaaa
     2161 catcttgtaa atttaagttg aattcattaa aataaattgg ctgttcagta agtattggtg
     2221 aatgattttc atataaaatg atatcattta aacgtttttg ttgtctgaat agatcgataa
     2281 ggtaaaagtt tagtcgcatg aataaaaaag aggataaaga ttgcttgctt gagggtttat
     2341 atatctgact caattgccac atttttatca agagtagttg ataatactca tcataattat
     2401 agctaatatt atattttttt aaaagatggt gtatgatttt gtggtgtttg ttgtatacgt
     2461 cattaaattt caagtagtca ttctccaagt tatacgtata acaaatattt ccggataaag
     2521 ttagaataaa atatttagaa aaatcattca tttgcgt
//
LOCUS       DAHHOJ010000185         2512 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.188, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000185 DAHHOJ010000000
VERSION     DAHHOJ010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.188"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(407..964)
                     /locus_tag="NBH32_002622"
     CDS             complement(407..964)
                     /locus_tag="NBH32_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000633422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HCD1516158.1"
                     /translation="MITTIVLTLCTFVSWLLIPHKITRYILGTLFTLALTLTIIGITA
                     NMTNHWGMEKKVITSNKKEIFTGGPKNNPANILIINEIGKNTNNYVMVYRDHPKDKKP
                     TTHFKPKMDKNNISESVKHQAKYEVKNTDKATQQTQKEVWVWKSDLYKSLLSFDESEN
                     ELIQSTTTVTVPKDTWVIINAKKDK"
     gene            complement(966..2483)
                     /locus_tag="NBH32_002623"
     CDS             complement(966..2483)
                     /locus_tag="NBH32_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000107169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HCD1516159.1"
                     /translation="MSTQKSNAMNLDIHGNQYKPKIIMSIILLATFSGALMQTSLGTA
                     LPTLMNDFDIDFSTAQQATTWFLLANGIMVPLSAFLATRISTRLLHVYSYGALLFGLI
                     LTAIAPSNQDGWLIFLAGRIVTAIAVGLMMPLLQIIIIYMFPIKQRGTAMGLSGLAVG
                     LAPAMGPTFAGWILNKNHEVLGITISDSWRNIFVLPIIIIAIAFILSFFFMKDVIPNR
                     KLKLDLYSLILSCIGFGLFLWGFSNVASEGWGNINYVILPIFLSIIVLTLFVIKQLKS
                     KDPFLDLRVFKSKGFMIATIGLIVVTMAMYGVEMMLPTYLQNIHGFSPFESGLTLLAG
                     ALMLGFISPISGILYNKIGIKRLAFVGFTILTLASVPFGFLTVNTSPTLILVLYAVRM
                     FGVAILMMPLTTNAMDALPVEKGTHATAVNNTARQVASSIAVALLTSVTQNIINNHMP
                     ADSLKESNPLKYAHLAQQASIDGFNTAFIIGIFFAIIGIILVPFLKMNIIESEDK"
BASE COUNT          861 a          393 c          396 g          862 t
ORIGIN      
        1 attgaaaata aaaaatatta tttgaataat aatgagattt tatccttata tcattcaggg
       61 aaggttatgg atactagctt ttcaacttta atgtatatag gattatttaa acatttttct
      121 agttatttaa gaaaacacga ataaaaaaca actgatgata acaagttaac aaattattaa
      181 atttaaatca ctctagttca taccaaaaag tctttaacac aaacaaaaaa ggaggaaact
      241 taaattcctc ctcaaccttt attacaccta taattctatt ttaacgtctt cgtccatttg
      301 ggcttcaaat tcatctagta gtgctcgtgc ttctgcttaa tttgaatcat acaaggtgtt
      361 ttttgttatt cctaaaattt ggcatagcta aataaatctg tattttctat ttgtcttttt
      421 tggcatttat gataacccaa gtatcttttg gaacggtaac tgtagttgtt gattgaatta
      481 attcgttttc actttcatcg aagcttagta atgatttata taaatctgat ttccaaaccc
      541 aaacttcttt ttgggtttgc tgtgttgcct tatcagtatt ctttacttca tatttagctt
      601 gatgcttaac ggactcagat atattatttt tatccatttt aggcttgaaa tgagttgtag
      661 gttttttatc cttaggatga tctctataga ccatgacata gttatttgta ttttttccta
      721 tttcattgat tattaatata ttagctggat tatttttagg accaccagta aaaatttctt
      781 ttttatttga tgtgattact ttcttttcca taccccaatg gttagtcata ttagctgtaa
      841 taccaatgat tgttagtgtc aacgcaagag taaataatgt tcctaatata tagcgtgtga
      901 ttttatgagg aattaataac caactaacaa aagtacataa agttaaaaca atcgttgtta
      961 tcatattatt tatcctcact ttcaataata ttcattttta aaaatggaac taaaatgata
     1021 cctataattg caaagaaaat gccaattata aatgcagtat taaagccatc gattgatgct
     1081 tgttgagcaa ggtgtgcata tttgagtgga ttgctttctt ttaaactatc tgcaggcata
     1141 tggttgttaa taatattttg agtaacagat gtcagtaatg ctactgctat agatgatgct
     1201 acttgtctag cagtattatt tactgctgta gcatgtgtgc ctttttcaac tggtaaagca
     1261 tccatcgcat ttgttgtgag tggcatcatt aaaattgcca caccaaacat tcttactgca
     1321 tatagaacaa gaataagtgt tggtgatgtg ttaactgtta aaaatccaaa tggaactgaa
     1381 gctaaagtta aaatagtgaa accaacgaac gctaatcttt taatgccaat tttattgtac
     1441 agtattcctg aaattggaga gataaatccc aacatcagtg ctccagctaa aagtgttaaa
     1501 ccagattcaa atggtgagaa accgtgtata ttctgtaagt aagtgggtaa catcatttca
     1561 acaccataca ttgccatagt tacaacgatt aaaccaattg tcgctatcat gaaaccttta
     1621 gatttaaaaa cgcgtaaatc caagaaagga tctttagatt ttaattgttt gataacgaac
     1681 aaggtaagaa caatgatact taaaaaaatt ggtaaaataa cgtaatttat atttccccat
     1741 ccttcgcttg caacgtttga aaatccccat aaaaataatc cgaaaccgat acaagaaagt
     1801 attagagaat agagatctaa tttaagttta cgattaggta taacatcttt catgaagaag
     1861 aagcttaata taaatgctat cgctataata atgattggaa gaacaaaaat attacgccat
     1921 gagtctgaaa ttgtaatacc tagtacctcg tggtttttgt ttaatatcca accagcaaaa
     1981 gttggaccca ttgcaggtgc gagacctact gctaaaccac tcagacccat tgcagtacca
     2041 cgttgtttta ttggaaacat ataaataatg atgatttgta acaatggcat cattaaacca
     2101 acagcaattg ctgtaacaat acgtccagct agaaatatta accagccatc ttgatttgat
     2161 ggtgcgattg cagtaagaat taatccgaat aatagggcgc cgtaagagta tacatgtaac
     2221 aatctagtag atatgcgcgt cgctaaaaat gccgaaagtg gtaccataat accatttgcg
     2281 agtaaaaacc aagttgttgc ttgttgagca gtactaaaat cgatatcaaa atcgttcatc
     2341 aaagtaggta gagcggtacc taatgaagtc tgcatcaatg caccagagaa tgtagctaat
     2401 aatataatgc tcattataat tttgggttta tattgattgc catgaatatc taaattcatt
     2461 gcattacttt tttgagtaga cataaagcaa gcctcctata ggtttttaaa at
//
LOCUS       DAHHOJ010000186         2509 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.189, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000186 DAHHOJ010000000
VERSION     DAHHOJ010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.189"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            130..>2509
                     /locus_tag="NBH32_002624"
     CDS             130..>2509
                     /locus_tag="NBH32_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001039417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cna B-type domain-containing protein"
                     /protein_id="HCD1516160.1"
                     /translation="MNKNVLKFMVFIMLLNIITPLFNKNDAFAARDISSTNVTDLTVS
                     PSKIEDGGKTTVKMTFDDKNGKIQNGDTIKVAWPTSGTVKIEGYSKTVPLTVKGEQVG
                     QAVITPDGATITFNDKVEKLSDVSGFAEFEVQGRNLTQTNTSDDKVATITSGNKSTNV
                     TVHKSEAGTSSVFYYKTGDMLPEDTTHVRWFLNINNEKRYVSKDITIKDQIQGGQQLD
                     LSTLNINVTGTHSNYYSGSNAITDFEKAFPGSKITVDNTKNTIDVTIPQGYGSYNSFS
                     INYKTKITNEQQKEFVNNSQAWYQEHGKEEVNGKSFNHTVHNINANAGIEGTVKGELK
                     VLKQDKDTKAPIANVKFKLSKKDGSVVKDNQKEIEIITDANGIANIKALPSGDYILKE
                     IEAPAPYTFDKDKEYPFTMKDTDNQGYFTTIENAKAIEKTKDVSAQKVWEGSQKVKPT
                     IYFKLYKQDDNQNTTPVDKAEIKKLEDGTTKVTWSNLPENDKNGKAIKYLVKEVNAQG
                     EDTTPEGYTKKENGLVVTNTEKPIETTSISGEKVWDDKDNQDGKRPEKVSVNLLANGE
                     KVKTVDVTSETNWKYEFKNLPKYDEGKKIEYTVTEDHVKDYTTDINGTTITNKYTPGE
                     TSATVTKNWDDNNNQDGKRPTEIKVELYQDGKATGKTATLNESNNWTHTWAGLDEKAK
                     GQQVKYTVEELTKVKGYTTHVDNNDIGNLIVTNKYTPEKPNKPIYPEKPKDKTPPTKP
                     DHSNKVKPTPPDKPSKVDKADQSKDNKTKPENPLKELPKTGMKIITSWITWVF"
BASE COUNT         1080 a          347 c          468 g          614 t
ORIGIN      
        1 tgtatatatt ttgcataata aaataataat atgaattttt gataaattcc attgaataag
       61 aactaaatta gtttataatt tatcattagt atcctatgga tatgacatag attataagga
      121 ggggttttta tgaacaagaa tgtgttgaag tttatggtct ttataatgtt attaaatatc
      181 atcacgcctt tatttaataa aaatgacgca tttgcagcac gagatatttc atcaacgaat
      241 gttacagatt taactgtatc accgtctaag atagaagatg gtggtaaaac gacagtaaaa
      301 atgacattcg acgataaaaa tggaaaaata caaaatggtg acacgattaa agtggcatgg
      361 ccgacaagcg gtacagtaaa gatagagggt tatagtaaaa cagtaccatt aactgttaaa
      421 ggtgaacagg tgggtcaagc agttattaca ccagacggag caacaattac attcaatgat
      481 aaagtagaaa aattaagtga tgtttcggga tttgcagaat ttgaagtaca aggaagaaat
      541 ttaacgcaaa caaatacttc agatgacaaa gtagctacga taacatctgg gaataaatca
      601 acgaatgtta cggttcataa aagtgaagcg ggaacaagta gtgttttcta ttataaaact
      661 ggagacatgc tgccagaaga tacgacacat gtacgatggt ttttaaatat taataatgaa
      721 aaaagatatg tatcgaaaga tataactata aaggatcaga ttcaaggtgg acagcagtta
      781 gatttaagca cattaaacat taatgtgaca ggtacacata gcaattatta tagtggatca
      841 aatgcaatta ctgattttga aaaagctttt ccaggttcta aaataactgt tgataatacg
      901 aagaacacaa ttgatgtaac aattccacaa ggctatgggt catataatag tttttcaatt
      961 aactacaaaa ccaaaattac gaatgaacag caaaaagagt ttgttaataa ttcacaagct
     1021 tggtatcaag agcatggtaa ggaagaagtg aacgggaaat catttaatca tactgtgcac
     1081 aatattaatg ctaatgctgg tattgaaggt actgtaaaag gtgaattaaa agttttaaaa
     1141 caggataaag ataccaaggc tcctatagct aatgtaaaat ttaaactttc taaaaaagat
     1201 ggatcagttg taaaggacaa tcaaaaagaa attgagatta taacagatgc aaacggtatt
     1261 gctaatatta aagcgttgcc tagtggagac tatattttaa aagaaataga ggcgccagca
     1321 ccgtatacat ttgataagga taaagaatat ccgtttacta tgaaagatac agataatcag
     1381 ggatatttta cgactattga aaatgcaaaa gcgatagaaa aaacaaaaga tgtttctgct
     1441 caaaaggttt gggaaggatc tcaaaaagtg aaaccaacga tttatttcaa gttgtacaaa
     1501 caagatgaca atcaaaacac aacaccagta gacaaagcag agattaaaaa attagaagat
     1561 ggaacgacaa aagtgacatg gtctaatctt ccagaaaatg acaaaaatgg caaggctatt
     1621 aaatatttag ttaaagaagt aaatgctcaa ggtgaagata caacaccaga aggatatact
     1681 aaaaaagaaa atggtttagt ggttactaat actgaaaaac caattgaaac aacatcaatt
     1741 agcggtgaaa aagtatggga cgacaaagac aatcaagatg gtaagagacc agaaaaagtc
     1801 agtgtgaatt tattggctaa tggagagaaa gtaaaaacgg tagatgtgac atctgaaaca
     1861 aactggaagt acgaatttaa aaacttaccg aagtatgatg aaggaaagaa aatagaatat
     1921 acagtgaccg aagatcacgt aaaagactac acaacagaca tcaacggtac gacaataacg
     1981 aacaagtata caccaggaga gacatcggca acagtaacaa aaaattggga tgacaataat
     2041 aaccaagacg gaaaacgacc aactgaaatt aaagttgagt tatatcaaga tggaaaagca
     2101 acaggaaaaa cggcaacatt aaatgaatct aataactgga cccatacgtg ggcaggatta
     2161 gatgaaaaag caaaaggtca acaagtaaaa tacacagtcg aggaattaac aaaggtcaaa
     2221 ggttatacaa cacatgtgga taacaatgat ataggtaact tgattgtaac gaataaatat
     2281 acgccagaaa aaccgaataa accaatctat cctgaaaaac caaaagacaa aacaccacca
     2341 actaaacctg atcattctaa taaagttaaa ccaactcccc cagataagcc atcaaaagtg
     2401 gataaggctg atcaatctaa agataataaa accaaacctg aaaatcctct aaaagaatta
     2461 ccaaaaactg gtatgaagat tataacttca tggattacat gggtattta
//
LOCUS       DAHHOJ010000187         2472 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.190, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000187 DAHHOJ010000000
VERSION     DAHHOJ010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2472
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.190"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..1928
                     /gene="abc-f"
                     /locus_tag="NBH32_002625"
     CDS             <1..1928
                     /gene="abc-f"
                     /locus_tag="NBH32_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein"
                     /protein_id="HCD1516161.1"
                     /translation="ILLQLNHISKSFDGEDIFTDVDFEVKTGERIGIVGRNGAGKSTL
                     MKIIAGVENYDSGNVSKIKNLKLGYLTQQMTLNSNATVFEEMSKPFEHIKRMESLIKE
                     ETDWLSKHANDYDSDTYKTHMSRYESLSNQFEQLEGYQYESKIKTVLHGLNFSEEDFN
                     KPINDFSGGQKTRLSLAQMLLNEPDLLLLDEPTNHLDLETTKWLEDYLRYFKGAIVII
                     SHDRYFLDKIVTQIYDVALGDVKRYVGNYEKFIQQRDLYYQKRMQEYESQQAEIKRLE
                     TFVEKNITRASTSGMAKSRRKILEKMERIDKPMLDAKSANIQFGFDRNTGNDVMHVKN
                     LEIGYQTAITKPISIEVSKGDHIAIIGPNGIGKSTLIKTIANQQKALNGDITFGANLQ
                     IGYYDQKQAEFKSSKTILDYVWDQYPLMNEKDIRAVLGRFLFVQDDVKKIINDLSGGE
                     KARLQLALLMLQRDNVLILDEPTNHLDIDSKEMLEQALQHFEGTILFVSHDRYFINQL
                     ANKVFDLTIDGGKMYLGDYQYYIEKVEEAEALKAHQEEQSVNVQAHKKSMEQSSYHNQ
                     KEQRREQRKLERQISECENEIETLETTILQIDEQLTQPEVYNNPQKANELAIQKQDSE
                     QKLEHAMSKWEELQQKL"
     gene            complement(2087..2200)
                     /locus_tag="NBH32_002626"
     CDS             complement(2087..2200)
                     /locus_tag="NBH32_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516162.1"
                     /translation="MVKTMSIENMRTYQQRVYNLWIKGVDKGIYCGYQVGK"
BASE COUNT          948 a          396 c          394 g          734 t
ORIGIN      
        1 tgatactttt acaactcaat catatatcaa aatcgttcga tggtgaagat atatttactg
       61 atgttgattt tgaagtgaaa actggcgaac gaattggtat cgtaggaaga aatggcgccg
      121 gtaaatcaac attgatgaaa attatagctg gcgtagaaaa ctatgattct ggaaatgtgt
      181 caaaaattaa aaacttaaaa ctaggttatt taactcaaca aatgacttta aattctaatg
      241 caacagtttt tgaagaaatg tcaaaaccat ttgaacatat taaacgaatg gaaagcttaa
      301 tcaaagaaga aacagattgg ttatcaaaac atgcaaatga ttacgatagt gatacatata
      361 aaacacatat gtcccgttat gaatctttat cgaatcaatt tgaacaatta gaaggatatc
      421 aatatgaaag taaaattaaa acagtacttc acgggttaaa ttttagtgaa gaagatttca
      481 ataaacctat caatgatttt agcggtggcc aaaaaacacg tttatcttta gctcaaatgc
      541 tattaaatga acctgattta ttacttttag atgaacctac taaccactta gatttagaaa
      601 cgacaaagtg gcttgaagat tatctacgtt attttaaagg tgcaatcgtc atcatcagcc
      661 atgatcgtta ctttttagat aaaatagtta ctcaaattta tgatgtggct ttaggtgatg
      721 tcaaacgcta tgttggtaat tacgagaaat ttatacagca acgggattta tactatcaaa
      781 aacgaatgca agaatatgaa agccaacaag cagaaataaa acgattagaa acttttgttg
      841 agaaaaatat tacacgtgct tcaacaagtg gaatggcaaa aagtagacgt aagattttag
      901 aaaaaatgga acgcattgat aaaccaatgt tagatgccaa aagtgcaaat attcaatttg
      961 gctttgaccg gaatacaggt aatgacgtca tgcatgtaaa aaatttagaa atcggttatc
     1021 aaactgcaat taccaaacct atcagtatag aggtctctaa aggcgatcat atagcaatca
     1081 ttgggccaaa tggtattgga aaatcgacct taattaaaac tattgctaat caacaaaaag
     1141 cgcttaatgg cgatattact ttcggcgcaa atttacaaat tggttattat gatcaaaaac
     1201 aagcagaatt taaatctagt aaaacgattt tagattatgt gtgggatcaa tatccgttaa
     1261 tgaatgaaaa agatattcgt gcagttcttg gacgtttctt atttgtacaa gacgatgtta
     1321 aaaagataat taatgattta tctggtggtg aaaaagcacg tttacaacta gcactactta
     1381 tgttgcagcg tgacaatgta cttattttag atgaacctac caaccacctc gatatagatt
     1441 caaaagaaat gttagagcaa gcactccaac attttgaagg aacaatacta tttgtttccc
     1501 atgatcgtta ctttattaac caattggcaa acaaggtatt tgatttaaca attgatggcg
     1561 gaaagatgta tttaggagat tatcaatatt acattgaaaa agttgaggaa gcggaagcgt
     1621 tgaaagcgca ccaagaagaa cagtcagtta acgtacaagc acataaaaag tctatggaac
     1681 aatcgtcgta tcataaccaa aaagaacaaa gacgtgaaca acgcaaactt gaacgacaaa
     1741 tatccgagtg tgaaaatgaa atagaaactt tagagactac tatcttacaa atagacgagc
     1801 aattaaccca accagaagtg tataacaatc cacaaaaagc aaacgaatta gctatccaaa
     1861 aacaagatag cgaacaaaaa ttagaacacg ctatgtctaa atgggaagaa ttacaacaaa
     1921 aactataaaa atatacataa ctctaactgt aaaatcgcta tagttagagt tattttttta
     1981 caaattttaa tttttttatt cacaataaaa acgctgatat tatagctatc cacagactta
     2041 tcctagttat ccacaagtaa attaacacta atcaatcatt tattttctat ttccccacct
     2101 gatatccaca ataaatgcct ttatcaacac cttttatcca caagttatac actctttgtt
     2161 gataagtacg catgttctct attgacattg tttttaccaa aatccattgt taagacattt
     2221 tctttatgaa ataaggctgg gacataaatc cctaaaaaaa cagcagtaag ataattttca
     2281 attagaaaat atctcactgc tgttctctat ttatacaata cttcgtattg aatggcttcg
     2341 ctttcctagg gtgccgtctc agcctcggtc ttcgactggc actgctccct caggagtctc
     2401 gccattaata ctacgtatta acatgtaatt ttacttttaa atactttaaa aaaataagac
     2461 actttcgtat aa
//
LOCUS       DAHHOJ010000188         2289 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.191, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000188 DAHHOJ010000000
VERSION     DAHHOJ010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2289
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.191"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(711..1640)
                     /locus_tag="NBH32_002627"
     CDS             complement(711..1640)
                     /locus_tag="NBH32_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001574632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCD1516163.1"
                     /translation="MLLFYIIGVLVGMLIPIQTSVNSRLSLYTKSPFYTSFISFSVGT
                     ICLIILNIIINPEVFTIHFYNNQSFNNTWIVGGLLGVSFLTGNLLLLPKLGATLTVIA
                     TVAGQIIMGVIIDTFGLFGATIHDFNLIKAIGVLLLIIGIVIMNQFNKNNLLLTDQKY
                     LLFWLLLGFIFGFFPPIQTTINSALASHTHSPAFASLVSFTIGSITLLILTAIFNRSL
                     KLNTYHLKFGKLKPIYFTGGILGMAFVTANIILMPHMGAALTTLIGMFGQILMGILID
                     HFGLFGSPKIAMTSRKTIGLLCILTGIILLRLF"
     gene            complement(1836..2060)
                     /locus_tag="NBH32_002628"
     CDS             complement(1836..2060)
                     /locus_tag="NBH32_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2922 domain-containing protein"
                     /protein_id="HCD1516164.1"
                     /translation="MTKTLELSFKSSLDKPVKLQLPQLNNIVTEQVARDSMNALVDLN
                     IIKSNSGAITKVHSAQIIDKTTTVLFEAKK"
     gene            complement(2077..2280)
                     /locus_tag="NBH32_002629"
     CDS             complement(2077..2280)
                     /locus_tag="NBH32_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001837138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516165.1"
                     /translation="MSKTNHITIVLSFTKLDTNGKQTEFKRRFANINPEVSNDQIKTF
                     SKLIERLTGETYNNIEVIKSVSI"
BASE COUNT          847 a          313 c          331 g          798 t
ORIGIN      
        1 agcatttggt ttagtcaaaa atatcagcat tttttataat ttaccttaat ttaatcgtca
       61 gtataaggta atttatttta tgaaacgatt tagcgcaatt aaatgtttca atattcaatg
      121 tgtatctaaa gtgattcatt gtcatagctt aacgctttca agttaacttc ttctaatatt
      181 tatacccatt tttcgaactt ttatcactaa aaaagaaaaa tttttataat ttttttaatt
      241 tagtaattcc cagtgcagtt ttaatgataa ggaaaacaca ctaatttagt gattaaaacc
      301 aatctatagc acgttaaagt gtctatacgt tattccgatt gataatgggt aacaaataat
      361 agaaagtacg ataattgatc atacgttata gcacctttat ttttcaaact cgactcaatg
      421 aatgaattaa agttgtgcat tttgacttat ttttaattaa ttgaataacc aactatgaaa
      481 tattagcacg aataacataa tttttgctat taatttggta taaattatta aaaaacaaca
      541 ttaatttagg tcttaagaat gagttgacta tcgaatttgc taaattaaaa tttgttgtgt
      601 caaagcatac tacccatatt agtacttatt aaaactaatt tagaccacaa aaaaatagct
      661 tatcactttt aaaaataatt acaagttaaa atgataagct aaaagttaat ttaaaataat
      721 cttaaaagta taatgcctgt caaaatacat aatagaccaa tagtttttct ggatgtcatt
      781 gctattttag gtgaaccaaa taatccaaag tgatctatca atatgcccat tagaatctga
      841 ccaaacatcc caataagtgt tgttaatgct gcacccatat gaggcattaa gataatgtta
      901 gctgttacaa aagccatgcc tagtataccg ccagtaaaat agataggttt tagtttgccg
      961 aatttcaaat gatatgtatt tagttttaaa gaacgattaa aaatagcggt taaaatcaat
     1021 agcgttattg acccaattgt aaatgatact aatgatgcaa aggctggtga atgagtatga
     1081 cttgctaaag cactattaat tgtcgtttga ataggtggaa agaaaccaaa aataaatcct
     1141 aatagaagcc aaaacagtaa atacttttga tcagttagta ataaattatt cttgttaaat
     1201 tgattcatta tgacgatacc gataatgagt aacaatacac caattgcttt aattaaatta
     1261 aaatcatgaa ttgtagcacc aaataagcca aatgtatcaa ttatgacacc cataataatt
     1321 tgacccgcaa ctgttgcaat tacagttaat gttgcaccta attttggcaa taacaataaa
     1381 ttgccagtta aaaagctaac cccaagcaaa ccaccgacta tccatgtatt gttaaatgat
     1441 tgattattgt aaaagtgaat agtaaatact tctggattga taatgatatt taaaataatt
     1501 aaacaaattg ttccaactga aaatgaaatg aatgaagtat agaaaggcga tttagtatac
     1561 agtgatagtc ttgaattgac agatgtttgg ataggaataa gcattccaac taggacacca
     1621 atgatataga aaagaagcat atgaataaag tctcactttc caaagttaat aataaaaatc
     1681 atatgtatac attatcatta tagagaaaat atacaactca tttacaataa tgttgaaaaa
     1741 aacagggcat taacacaaca aaaacaccct agacaatcat catgaaagtc tagggtgtta
     1801 gtatttatac taaattaatt gcgctttctt tatatctatt tctttgcttc aaataaaacg
     1861 gtcgtcgttt tatcaataat ttgagcagaa tgtactttgg tgatagcgcc actatttgac
     1921 tttataatat ttaaatctac taatgcattc atactatcac gtgcaacttg ctcggttacg
     1981 atgttgttta attgtggtaa ttgcaatttg acaggtttgt ctagtgaaga tttaaaactt
     2041 aattcaagtg ttttggtcat gaaagtgtcc tcctgattaa attgatacag atttgatgac
     2101 ttcgatgttg ttgtatgttt ctccagtaag tcgttcgata agtttgctaa atgttttaat
     2161 ttggtcgttt gatacttcag gattaatatt ggcgaatcga cgcttaaatt ctgtttgttt
     2221 accatttgta tctagttttg taaatgataa tacaattgta atgtgatttg ttttactcat
     2281 attttaaaa
//
LOCUS       DAHHOJ010000189         2277 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.192, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000189 DAHHOJ010000000
VERSION     DAHHOJ010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.192"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            280..483
                     /locus_tag="NBH32_002630"
     CDS             280..483
                     /locus_tag="NBH32_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sterile alpha motif-like domain-containing
                     protein"
                     /protein_id="HCD1516166.1"
                     /translation="MTFYNFIMGFQNDNTPFGILAEHVSEDKAFPRLEERHQVIRAYV
                     MSNYTDHQLIETTNRAISLYMAN"
     gene            complement(480..716)
                     /locus_tag="NBH32_002631"
     CDS             complement(480..716)
                     /locus_tag="NBH32_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516167.1"
                     /translation="MDNHNQCNYVNPQNVSLDWECFIISKSEMLLDGVPNELINTWLD
                     KDIITPFSIRNDEINFKTKDIWDALIHHNWYYSN"
     gene            941..1522
                     /locus_tag="NBH32_002632"
     CDS             941..1522
                     /locus_tag="NBH32_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCD1516168.1"
                     /translation="MAKTLYLMRHGQTLFNFKGLIQGFGDSPLTELGIAQAQKARSYY
                     ETKGINFDLYASSTQERASDTLENVAPNQSYQRFKGLKEWHFGLFEGESVYLFDNLYK
                     PEDLFGDRIVPFKGEARQQVEDRIVKTLHDIMSQTKNNALVVSHGTIMGVFLRYCLKL
                     DEALKHNIGNCNILKFEYDNGTFKFVELIDPNL"
     gene            complement(1595..2212)
                     /locus_tag="NBH32_002633"
     CDS             complement(1595..2212)
                     /locus_tag="NBH32_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid transporter"
                     /protein_id="HCD1516169.1"
                     /translation="MVTAIIHGFILAIGLILPLGAQNVFIFNQGANQPKYRYVLPAII
                     TAGLSDSLLIIIAVVGVSIIIMSLPVLQAIIYIVGLIFLMYMAWTIWHDKPSTDGEAQ
                     IMSPMTQVSFALSVSLLNPHAILDTIGVIGSSAALYSGSNKIAFTIACISVSWLWFFL
                     LAILGKMVGSIDKTGKLLTIINKISSIIIIIVALMILQKLIQLIF"
BASE COUNT          844 a          335 c          346 g          752 t
ORIGIN      
        1 taggtaaata aaaagtatag ttatttttaa aatattattg tagaatgcta aagtttatta
       61 attcaaacaa gtgagcatat atagaggtgt gtagtattaa tatgaaaaga atatttacaa
      121 actaaaaacc tttaaaattg tataattcgt taatattttt agaatcagat aacaaaaaaa
      181 tgtgcaaaat cacttttgca catttacaaa tactagtttt ctgataagat taataataac
      241 actttaaata gcgcttaata aatgaagggg gtaagtctta tgacgtttta caatttcatc
      301 atgggttttc aaaatgataa cacaccattt ggcatattgg ccgaacacgt tagtgaagat
      361 aaagcattcc ctcgattaga agaaagacac caagtaatta gagcatatgt gatgtctaat
      421 tacacagatc atcaattaat tgaaactaca aatagagcta ttagcttata tatggcaaat
      481 taatttgagt agtaccaatt atgatgtatt agtgcatccc aaatatcttt tgttttaaag
      541 tttatttcat catttcttat cgaaaatggt gtaataatgt ctttatctaa ccaagtgttg
      601 ataagttcat ttggtacacc atctaacaac atttcacttt tactaattat aaaacattcc
      661 cagtcaagtg aaacattttg tggattcaca taattacatt gattatgatt atccataaac
      721 actcactcct ttaaaattct gtactcttca ttgcgtttta ccccgtcaca ttatctttta
      781 aactaaaatc atcattactt atgaaaaaaa tgttcatcaa aagcaaaggt ttttgctacc
      841 gaaaaagttt aaataatggt ttaatatatt tggtactcat tttaataaaa agagaataca
      901 ttttgagcta tcaatacttt ttattgaaga ggtgttattg ttggctaaaa cgttatattt
      961 aatgcgccac ggacaaactt tgtttaattt taagggacta attcagggat ttggagattc
     1021 gccgctaaca gaacttggaa ttgctcaagc tcaaaaggca cgtagttatt atgaaactaa
     1081 ggggataaac ttcgatttat atgcatcatc aacgcaagaa cgcgcaagtg acacacttga
     1141 aaatgttgca cctaaccaat cgtatcaacg ttttaaggga ctgaaggaat ggcattttgg
     1201 attatttgaa ggtgagtcag tctatctatt tgataatcta tacaagcctg aggacttatt
     1261 cggagatcga attgttcctt tcaaaggaga ggcaaggcaa caagttgaag atcgcattgt
     1321 gaaaacttta catgacatta tgtctcaaac aaagaataat gcattagtcg tgagtcatgg
     1381 aacaataatg ggagtatttt taagatattg ccttaaacta gatgaagcat taaagcataa
     1441 tatcggtaat tgtaatatcc tgaaatttga atatgacaat ggaacattta aatttgttga
     1501 gttaattgat ccaaatttat aataaataaa gcgttataca tagacacaag tatgtctttt
     1561 gtataacgct tttaaaatac aactatataa cgctttagaa tattaattga ataagttttt
     1621 gtaatatcat cagagcaaca ataataatta ttatgcttga tattttattg attattgtta
     1681 ataattttcc ggttttatca attgatccaa ccatttttcc taaaattgcg agtaaaaaga
     1741 accataacca tgaaacgcta atacaagcga ttgtaaacgc aattttattg ctgccactat
     1801 ataatgcagc actactacca attacaccaa ttgtatctaa aatagcatgt ggattgagta
     1861 atgaaactga taaagcaaaa cttacttgtg tcattggaga cataatttga gcttctccat
     1921 ctgttgaggg tttatcatgc caaatggtcc aagccatata catcaagaaa attaaaccaa
     1981 ctatataaat aattgcttga agtacaggta aagacataat aatgatagat actcctacca
     2041 ctgcaataat gataagtaag ctgtctgaca acccggctgt aattattgca ggcaatacat
     2101 atctatattt tggttgatta gctccttggt taaaaataaa tacattttgt gcacctaaag
     2161 gtaaaattag accaatcgct aagataaatc catgaataat tgcggttacc attaagtttt
     2221 gacaccccta aaacatagaa tattctgaaa aaacttattg atatcatacc aaaaaaa
//
LOCUS       DAHHOJ010000190         2188 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.193, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000190 DAHHOJ010000000
VERSION     DAHHOJ010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.193"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            228..638
                     /locus_tag="NBH32_002634"
     CDS             228..638
                     /locus_tag="NBH32_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000612779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YolD-like family protein"
                     /protein_id="HCD1516170.1"
                     /translation="MIPNPNAPDEYKYETDYRKIPRKYLNPKIPQGRGKIKWQAFATL
                     PQQFEILEQIIKDQNKIEKPLLTHDSLDNLDQIFQIKIQNDELCTISYWEDGSISNYT
                     GKILKIDTINGYFTFYDSTNTKHTFFNTNVYNIS"
     gene            complement(667..1032)
                     /locus_tag="NBH32_002635"
     CDS             complement(667..1032)
                     /locus_tag="NBH32_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000410574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1516171.1"
                     /translation="MGKFNRQEQLKQLHAERKVKTEQKVSKAINELVRKNEEINFNIV
                     AKHSNVSKATLYNNSKIRKRIEELREQSKDIFVHKNNSDGKDALISSLKRRVKSLENE
                     KKRLKDEINTLYNKIYEDI"
     gene            complement(1041..>2188)
                     /locus_tag="NBH32_002636"
     CDS             complement(1041..>2188)
                     /locus_tag="NBH32_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCD1516172.1"
                     /translation="LKAIRRFLNDIASQNYDIAPSEDIRFLIYPQDFPKREKKNSSQI
                     DYIPDFVLEQLFEHINDLHKDLIPVVWIAFKTGLRISDVLTLKNNCLAKVNGKFSIIT
                     DIAKTFVKGHRIPIDNELADIVAVLIANSKIKSTKDNNPNNYIFAIHKGKRKGMPFTQ
                     HMVRAHLNHLAKTKNILDEQGEVFHFKTHQFRHTYAVKLLNGGADILTIQELLAHSSP
                     EMTLRYAKLLDDTKRKAFESVIDQGAFSFDVDGKIKNIQHSSELSEKALNSLWQEHKL
                     NAMDNPYGTCHARLSGDCPYMEAPPCLTCNSGKPCKDLAIGFSDLDVEKYELHIKSTV
                     KSIELAKNNNRQDIVEKHTNILNKYEEILGNIKDGNIIFGRSNRIKV"
BASE COUNT          725 a          344 c          258 g          861 t
ORIGIN      
        1 atatatgttc tattttttta tgttcacatt ttttaaaaga gaaaatacta ttaacatacc
       61 aaacatcata attccagtta ctataattat aaatgctcga taatcttact caacttttcc
      121 atatagaaaa taacttattt taaattaatt aattaatcac attaaaaaat tatataatcc
      181 ttgccaaaaa agaacatacg ttctacaatg atgatgaggt ggtaaacatg attccaaatc
      241 ctaatgcgcc agatgaatat aaatatgaaa ctgattatag aaaaatacca agaaaatatt
      301 taaaccctaa aatccctcaa gggcgtggaa aaatcaaatg gcaagctttt gcgactctcc
      361 cgcaacagtt tgaaatttta gaacaaatta taaaggatca aaataaaata gaaaaaccat
      421 tgctaacaca tgattcatta gataatttag atcagatttt tcaaataaaa attcaaaatg
      481 atgagttatg tacaatctca tattgggaag acggcagcat ttcaaactat acaggaaaaa
      541 ttttgaaaat tgatacaatc aatggttatt ttacatttta cgattctaca aatactaaac
      601 atactttttt caatactaat gtgtataata tctcttaaac ttgaattatt tctctttacc
      661 tgtgtattaa atatcttcat atattttatt atataatgtg tttatttcat ctttgagcct
      721 ctttttctca ttttctaaag attttactct cctttttaat gaagaaatta atgcgtcttt
      781 accgtcactg ttatttttat gtacaaatat gtctttactt tgttcacgta actcttcgat
      841 tctctttcga attttactat tattatataa agtagcttta gatacatttg aatgcttagc
      901 aacaatatta aagttaattt cttcattttt tcgaaccaat tcatttattg ctttagaaac
      961 tttttgctcg gttttaactt ttcgttctgc atgtaattgt tttaattgtt cttgtctatt
     1021 aaattttccc atcttaacct ctatactttt attctattac ttctcccgaa tataatgttt
     1081 ccatctttta tattacctaa tatttcttca tatttattta atatattagt atgtttttca
     1141 actatatctt gtctattatt atttttagct aattctatgg atttgaccgt tgatttgata
     1201 tgtaattcat atttttcaac atctaaatca gaaaatccaa tagctagatc cttacatggt
     1261 ttcccagaat tacaagttaa gcatggcgga gcttccatat atggacaatc accgctcaat
     1321 ctagcatgac aagtaccata aggattgtcc attgcgttta atttatgttc ttgccataaa
     1381 gaattcaatg ctttttctga taattcacta ctatgctgta tatttttaat tttaccgtca
     1441 acatcaaaac taaaagcccc ttgatcaata actgattcaa aagcctttct ttttgtatca
     1501 tcaagtaact tagcatatct gagtgtcatt tctggtgagg aatgtgctaa taattcttgt
     1561 atggttaata tatctgctcc accatttaac aatttcactg cataagtatg tctaaactga
     1621 tgagttttga aatggaagac ttcaccttgt tcatcaagaa tattttttgt ttttgctaaa
     1681 tgatttaagt gtgctctgac catatgctgt gtaaagggca ttccttttct ttttccttta
     1741 tgaatagcaa aaatataatt attagggttg ttatcttttg tacttttaat tttagaattg
     1801 gctattaaaa ctgctactat atcagctaat tcattatcga ttggtatcct atgtccttta
     1861 acaaaagttt tagcaatatc agtaattatt gaaaactttc cattaacttt tgcaagacaa
     1921 ttatttttta atgttaatac atcagaaatt cttagtcctg ttttaaaagc tatccaaact
     1981 acaggtatta aatctttgtg caaatcattt atatgctcaa aaagctgttc caatacaaaa
     2041 tctgggatat aatcaatttg gctactattt ttcttttcgc gcttaggaaa gtcttgagga
     2101 taaattaaaa aacgtatatc ttcagagggg gctatatcat aattttgact agcaatatca
     2161 tttaagaacc ttctaattgc ttttaatt
//
LOCUS       DAHHOJ010000191         2156 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.194, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000191 DAHHOJ010000000
VERSION     DAHHOJ010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.194"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..566)
                     /locus_tag="NBH32_002637"
     CDS             complement(<1..566)
                     /locus_tag="NBH32_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000681156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516173.1"
                     /translation="MKHKSVYLLCFLILPILLLTSVSAYAVSNPVGEPSHAVKPKIEK
                     YDLSHYRSIYSEKGDWNPFGQEEISRQINNVSDFFFSMTKILAGVTDYAIENLFQLDV
                     INDFADKIGDFVGDIYQKLLSNLALTLFIIVCFNAFIIFSVQGNAREALKRAFLIMCL
                     IGFGVGILANAGNIIRGTNNIGKDLNNI"
     gene            complement(571..1929)
                     /locus_tag="NBH32_002638"
     CDS             complement(571..1929)
                     /locus_tag="NBH32_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsK"
                     /protein_id="HCD1516174.1"
                     /translation="MAMNEIALFKGIRIQPYQKYIDFMFAGALAFIFLVYRIYILFID
                     YKSVNKKLDILTLFAYFKPHLLYLLIGTAIIFVICKLIAQFLIRFKTKDLAEMIETQG
                     FHNRTVIKKTTEYIPIDYKQTEYDYYPTMFYKRGKKSFVIHIKKDGSRFQDNYLELET
                     IIEPMFNSELVEKKHIGRYLRYEFMPLKYKKRIVMNGEQESNTIFYDTKIAITHQITW
                     DFVKAPHGLVTGITGGGKTYFLFYVIRELFRRHSEVRLLDPKVSDLSFMKRVIGDDKV
                     ADTKGQILKQLREANNEMEERFRLMNDSSDYKIGNDFRNFDMRPYFIIFDEVTAFTST
                     LDKKELQEMNDYLINIIMKGRQAGVFMFLTAQRPDADVIKGNVRDQLGLRVSLGNLSN
                     DGYRMTFGQTDKEFQTIHDSDIGRGYISILGQYNEPILFDAPLMEQYDFVEDVKQILN
                     KE"
BASE COUNT          804 a          391 c          229 g          732 t
ORIGIN      
        1 attatattat ttaagtcctt accaatattg tttgtaccac gtataatatt acctgcattc
       61 gcaagtatac caacaccaaa accaattaaa cacataatta aaaacgctcg ttttaatgct
      121 tctcttgcat taccttgtac actaaaaata ataaatgcgt taaagcaaac aataataaat
      181 aatgttaatg ctaaattact caataacttt tgatatatat cgccaacaaa atcgccgatt
      241 ttatccgcaa aatcattaat aacatctagt tgaaataaat tttcaatcgc ataatctgta
      301 acacctgcta aaatcttagt catagaaaag aaaaaatctg acacattatt aatttgtcgt
      361 gaaatttctt cttgaccaaa cggattccag tcgccttttt cactatatat tgaacgataa
      421 tgtgataaat catacttttc aatttttggc ttaactgcat gtgagggctc tccaactgga
      481 ttagaaacag cgtaagcact cacagatgtt aaaagcagaa taggcaaaat taaaaagcat
      541 aaaagataca cacttttatg cttcatcaaa tcactcctta tttaatattt gtttcacatc
      601 ttctacaaaa tcatactgtt ccattaaagg tgcatcaaat aaaattggtt cattatattg
      661 tccaagtata gaaatataac ctcggccgat atcactatca tgaatcgttt gaaattcttt
      721 atcagtttga ccaaaggtca tacgataacc atcatttgac aaattaccta aagacacacg
      781 taaccctaat tggtcacgca cattaccttt aatcacatct gcatctggtc tttgtgctgt
      841 taaaaacata aatacaccag cttgacgacc tttcataata atattaatta aataatcatt
      901 catttcttgt aattcttttt tatctaaagt tgaagtaaaa gccgttacct catcaaaaat
      961 aatgaaatag gggcgcatat caaaattacg gaaatcattc ccgattttat aatccgaact
     1021 atcattcatt aatctaaaac gctcttccat ttcattatta gcttctctca attgttttaa
     1081 tatttggcct ttagtatctg caactttatc atcaccaata acgcgcttca taaagctcaa
     1141 atctgaaact tttgggtcta ataaacgtac ttctgaatgt ctacggaata actcacgaat
     1201 cacataaaat aaaaaatatg ttttaccacc tccggttata cctgtaacca atccatgagg
     1261 tgctttcaca aaatcccaag taatttgatg cgtaatagca atttttgtat cataaaaaat
     1321 tgtatttgat tcttgttcgc cattcataac aatacgcttt ttatatttaa gtggcataaa
     1381 ttcatatctc aaataacgac caatatgctt tttttctact aattcagaat taaacattgg
     1441 ttcaataatc gtctctaact ctaaataatt atcttgaaac ctcgaaccgt ctttcttaat
     1501 atgaataaca aaagattttt taccacgctt ataaaacatt gttggataat aatcatattc
     1561 agtttgttta taatcaatag ggatatactc agttgttttc ttgattactg tacgattatg
     1621 aaacccttgt gtttcaatca tttcagccaa atcttttgtt ttaaaacgta ttaaaaactg
     1681 agcaataagc ttacatatca caaatataat tgctgtacca atcaataaat acaataaatg
     1741 cggtttaaaa tatgcaaaca acgtcaatat atccaatttt ttattaactg atttataatc
     1801 aataaacaaa atgtatatac gatataccaa aaaaataaat gctaatgcac ctgcaaacat
     1861 aaaatcaata tacttctgat aaggctgtat acgtatacct ttaaataaag caatttcatt
     1921 cattgccatt atatgcactc ctttaaaaat aaaaagcata cacaaataat gtgcatgctt
     1981 aaaacaaaat attaattttc caattgttct tccatatttt tattataatc agtaatactt
     2041 ttctcaaaac atcgatgtgc aaaaaataaa agaattattg atacaataaa taaaaacatc
     2101 ataataaaaa gctttctctt ccaagatttc cattgataat actcaatata atcatt
//
LOCUS       DAHHOJ010000192         2156 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.195, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000192 DAHHOJ010000000
VERSION     DAHHOJ010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2156
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.195"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            102..1337
                     /gene="istA"
                     /locus_tag="NBH32_002639"
     CDS             102..1337
                     /gene="istA"
                     /locus_tag="NBH32_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCD1516175.1"
                     /translation="MRHDIYEGVLFYIMKGIKPNYAELGRQYNCDPRTVKKYYEAGKE
                     NELERLKKRQQNKKASKLDPFKEIINKKIELGCTAMAIFKYIEKKGYEGKYTILREYC
                     KNKKQNETKKATIRVETNPGIAAQVDWKEDMVMHDKFGRTYQFNIFLYVLHYSKMKYI
                     TLTWDRKQDTLFECLKDAFEYTEGVPKEIWFDNMRTVVDRPRTQYKKVVFNNLFYQFS
                     KDANFEPIACRPYRPQTKGSVESLAKFVEQRLRPYDYEFYDAVELIGLVNDLCHELNH
                     LEISQATEQRPIDVFNYEEKEHLNSFNAKLLDTYIENECIRIVSKESMINFRKGKYSV
                     PTKYIGEEVQVIFNNSTDELLIYYDGELIRRHNLSERKFNYIVEDMSEILKSDVFKHK
                     DDKEILTYIENSLLLYDEI"
     gene            1349..2116
                     /gene="istB"
                     /locus_tag="NBH32_002640"
     CDS             1349..2116
                     /gene="istB"
                     /locus_tag="NBH32_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001095317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCD1516176.1"
                     /translation="MNTNHQKLLNNFEILKLKKFKDYYPNYIELLSKNEKSLTEILID
                     LTEKEIEYQSELKFKRAVNSARFPKIKYLHDFDFMFQPSINQQEILTLKSMHFLEDSI
                     NICFLGNSGVGKTHLAISLGIEACKQNIKTRFYTFKELIDLLTVSDSKGIINKTLKQL
                     SRIELLIIDEIGYTPITKEQADLFYQLMSLRYEWKSTIITTNIPFSSWGESFSNKIVS
                     AAIIDRLVHHSKVFKITGESYRLKDYKTEKSLNIRQS"
BASE COUNT          871 a          289 c          332 g          664 t
ORIGIN      
        1 gtaggaaaat ggcgaaatga aaatgctgtt tttcctacat tttttctttt ttaaattata
       61 taatcatgtc ttaactcaat cttcgattgg aggttatttg attgagacat gacatttatg
      121 aaggagtgct attttacatt atgaaaggta ttaaaccaaa ttatgccgag cttggtcgac
      181 aatataattg tgatccaaga acagttaaaa aatattatga agcaggaaaa gaaaatgaat
      241 tagaacgatt gaagaaaaga caacaaaata agaaagcatc aaaactagat ccatttaaag
      301 aaattattaa taaaaaaatt gaattaggat gtacagctat ggccattttt aaatatatag
      361 agaaaaaggg ttatgaaggt aaatatacta ttctacgaga atactgtaaa aataaaaaac
      421 aaaatgagac taaaaaagca actatacgcg tagaaactaa tcccggtata gctgctcaag
      481 tagactggaa agaagatatg gtaatgcatg ataagtttgg cagaacttat caattcaaca
      541 tctttcttta cgttctacac tattcaaaaa tgaagtatat cacattaact tgggatagaa
      601 aacaagatac cttatttgaa tgtttaaaag acgcttttga atacaccgaa ggggttccaa
      661 aagaaatctg gttcgataat atgagaactg tagttgatcg acctagaaca caatataaaa
      721 aagtcgtctt taataattta ttttatcaat ttagtaagga tgccaacttt gaacctattg
      781 cttgtagacc ctatcgtcct caaacaaaag ggtctgttga atcattagct aaatttgttg
      841 aacagcgttt aagaccatac gattatgaat tttatgatgc tgtagaactt attgggctag
      901 taaacgattt atgtcacgaa ttgaatcact tagaaatttc acaagcaaca gaacaacgac
      961 ctatcgacgt tttcaattat gaagaaaaag aacatttaaa ttcttttaat gccaagttac
     1021 tagatactta catcgaaaat gagtgtataa gaatcgtttc taaagaatcg atgattaact
     1081 ttagaaaagg taaatactcg gtacctacta aatacattgg tgaagaagta caagtgatat
     1141 tcaataattc tactgatgaa ttgctgattt attatgatgg tgaattaata agacggcata
     1201 atctatccga aagaaaattt aattatatcg ttgaagatat gagtgaaata ttaaaatcag
     1261 atgtatttaa acataaggat gacaaagaaa ttcttacgta tatagaaaat tcattattac
     1321 tgtatgacga aatttaggag gactttcgat gaatacgaac catcaaaaat tacttaataa
     1381 ctttgaaata ttaaaactaa aaaaattcaa agattattat ccaaactata ttgaattact
     1441 ttctaaaaat gagaaatctt taactgaaat attgattgat ttaacggaaa aagaaataga
     1501 atatcaatca gaattaaaat ttaaacgtgc tgtgaattca gcacgtttcc ctaaaataaa
     1561 atatttacat gattttgatt tcatgtttca acctagtata aatcaacaag aaatactcac
     1621 tttaaaatct atgcattttt tagaagatag tataaatatt tgtttcttag gtaatagtgg
     1681 tgttggtaaa acacacctag caatctcatt aggaatagag gcatgtaagc aaaatataaa
     1741 gactagattc tatactttca aagaattaat agacttatta actgtctcag attccaaagg
     1801 aatcattaat aaaacattaa agcaattaag cagaatagag ttacttatca ttgatgaaat
     1861 tggttatact cccatcacca aagaacaagc tgatttattc taccagttaa tgtcactaag
     1921 gtatgagtgg aaatcaacaa taattacgac taatattcca ttttcgagtt ggggagagtc
     1981 atttagtaat aagattgtat cagcagctat aattgatcgg ttagttcacc attcaaaagt
     2041 atttaaaatt actggagaat catatcgatt aaaagattat aaaaccgaaa aatcattaaa
     2101 tatacgacaa tcttaaaccg ctaaaaaacg acattttcaa accgccataa aacagc
//
LOCUS       DAHHOJ010000193         2145 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.196, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000193 DAHHOJ010000000
VERSION     DAHHOJ010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2145)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.196"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(270..1829)
                     /gene="rny"
                     /locus_tag="NBH32_002641"
     CDS             complement(270..1829)
                     /gene="rny"
                     /locus_tag="NBH32_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002459258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HCD1516177.1"
                     /translation="MNLLSLLLILLGIILGVVGGYVVARNLLLQKQSQARQTAEDIVN
                     QAHKEADNIKKEKLLEAKEENQILREQTEAELRERRSELQRQETRLLQKEENLERKSD
                     LLDKKDEILEQKESKIEEKQQQVDAKESSVQTLIMKHEQELERISGLTQEEAINEQLQ
                     RVEEELSQDIAVLVKEKEKEAKEKVDKTAKELLATAVQRLAADHTSESTVSVVNLPND
                     EMKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVILSGFDPIRREIARTALVNLVSDG
                     RIHPGRIEDMVEKARKEVDDIIREAGEQATFEVNAHNMHPDLVKIVGRLNYRTSYGQN
                     VLKHSIEVAHLASMLAAELGEDETLAKRAGLLHDVGKAIDHEVEGSHVEIGVELAKKY
                     GENETVINAIHSHHGDVEPTSIISILVAAADALSAARPGARKETLENYIRRLERLETL
                     SESYDGVEKAFAIQAGREIRVIVSPEEIDDLKSYRLARDIKNQIEDELQYPGHIKVTV
                     VRETRAVEYAK"
BASE COUNT          609 a          414 c          298 g          824 t
ORIGIN      
        1 tttttacatt gcctaagaac ttaatgttcc agcctctttt ttattggata gaaaagcaat
       61 catctttata ggtttacata atataaatat agtaattatg aagcctaaaa catttttatt
      121 aaaagcaata acttcttatt ttatatacgg tccgacttac tttcaaataa ctactgttta
      181 atatagttat taccaataaa gtgctgtcta actagattga gactacaact taaaaaagct
      241 ccctcactaa tttgtgaggg agacaaaaat tatttcgcat attctactgc tctagtctct
      301 cgaacaactg tcaccttgat atgaccagga tattgtaatt catcttcaat ctgattttta
      361 atatctctag ccaatcgata agattttaaa tcatcaattt cttcaggaga tacaatcact
      421 cggatttctc tacctgcctg aatcgcaaat gctttttcta caccatcata actttctgat
      481 aacgtttcta aacgttctaa tcgacgaata taattctcta atgtttcttt tcttgcacct
      541 ggacgagccg cagacaatgc atctgcagca gcaacaagga tagatataat agatgtaggt
      601 tcaacatcac catgatgaga atggattgca ttaataactg tttcattttc accatatttt
      661 ttcgctaatt ctacaccgat ttcaacatga ctaccttcta cttcatgatc aattgcttta
      721 ccaacatcat gtaaaagtcc agctcgtttc gctaatgtct catcttcgcc tagctcagca
      781 gctaacatac tagcaagatg cgcaacttca attgaatgtt taagtacatt ttgaccgtaa
      841 cttgtacgat agtttaaacg ccctacaatt tttactaagt caggatgcat attatgtgcg
      901 tttacttcaa atgtagcttg ttcacctgct tctctaataa tatcgtctac ttcttttcta
      961 gctttttcga ccatatcttc aattctacct ggatgaatac gtccatcaga tactaagtta
     1021 acaagtgctg ttctagcaat ttctcttctt attggatcaa aaccagataa tataaccgct
     1081 tctggtgtgt catcaataat taaatcaatg ccagttaaag tttcaagtgt gcggatgttt
     1141 cgtccttctc gtccaatgat tcgacctttc atctcatcat taggtaagtt aactactgat
     1201 accgttgatt cacttgtgtg atctgctgct aatctttgta ctgctgtagc taataattct
     1261 tttgctgttt tatcaacttt ttctttagct tctttttctt tttctttaac aagtactgca
     1321 atatcttgtg acagttcttc ctctactctt tgaagttgct cattaatagc ttcttcttga
     1381 gtgagaccgg agatgcgttc taattcttgt tcatgcttca ttattaacgt ttgaacacta
     1441 ctctcttttg catctacttg ttgttgtttt tcttcaattt ttgattcttt ttgctctaaa
     1501 atctcatctt ttttatctaa tagatcagat ttacgctcta agttttcttc tttttgaaga
     1561 agtcgggttt cttgtctttg aagttcgcta cgtctttctc gtagttctgc ttcagtttgt
     1621 tctcttagga tttggttttc ttcttttgcc tcaagtaatt tctctttttt gatattgtca
     1681 gcttctttat gtgcttgatt tacaatatct tcggcagttt gtctagcttg tgattgcttt
     1741 tgaagcaaca aatttcgggc aacaacatac cctccaacaa ctcctagaat gatccccagc
     1801 aaaatgagta ggaggcttaa taaattcaca caaacacctc cttttctagg gtttgctctt
     1861 tatatgaaat tcaatatgac tttatgaaat tatatataaa tcatacaaat taattgtacg
     1921 gtttatagga gataagtgtc aagttatcaa tttgcacttt tgcactatat tgaatgctat
     1981 ttctactatt ttctaaagtt ttgaagctcg attataaaac tattgataat gtaataataa
     2041 ataaaataaa aaaccttata gtctcgattt gtagtgcatc ccataaagtt agatattatt
     2101 ctagctttat gagatgaata tcactttcaa tcaatcctat aagga
//
LOCUS       DAHHOJ010000194         2115 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.197, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000194 DAHHOJ010000000
VERSION     DAHHOJ010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.197"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..190
                     /locus_tag="NBH32_002642"
     CDS             <1..190
                     /locus_tag="NBH32_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000810447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516178.1"
                     /translation="FDTYDPKKDSYKVFVQFDERIETDGDDKIEHRQTSAQLDLVRTA
                     EGWRIDNLKRFNLKPLGR"
     gene            246..602
                     /locus_tag="NBH32_002643"
     CDS             246..602
                     /locus_tag="NBH32_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001255381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystatin-like fold lipoprotein"
                     /protein_id="HCD1516179.1"
                     /translation="MRRWFVLILGLVILLSACGQKYDKEIDEVTKLEKESIQDVKNTK
                     KYKNVERSKSYYKIYNDGEVIIMTYMPFKDSNTKVSRVYKINQTSDKYEEDSNIDAEK
                     FEKDNKPVYEENNMKK"
     gene            711..1214
                     /locus_tag="NBH32_002644"
     CDS             711..1214
                     /locus_tag="NBH32_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000746376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1516180.1"
                     /translation="MKKRKKRKELDYKTRLAIAKYLNDGLNIIQISKILGRDDAVLYR
                     EVNKRSVDGVYNPLLAEEDAIKSRSISDEQVAKRRRLNDETKAYIEEKLSLKWSPRQI
                     ASQIEKDTGYYISYPTIYRYIRNGLVKVDVKRDMRQAGKKYNKSSEKRGKLDVGSRVI
                     KYRLKKY"
     gene            1195..1731
                     /locus_tag="NBH32_002645"
     CDS             1195..1731
                     /locus_tag="NBH32_002645"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HCD1516181.1"
                     /translation="MGSKSINNREEFGHYEIDTVWSVRPSTYCLLTIIERKTRYLYAS
                     RLNTRKSNVVCNEIINIMKPLLPKSITMDRGKEFALFEQIEDELNCSIYFSDLGCPYQ
                     RGSIENANGLLRQYYPKGTDFANITQKSLDEAVKQINARPRMIFDYKSSEEMLKYHVS
                     TQNCEPILNDCVRHEPVN"
BASE COUNT          807 a          240 c          374 g          694 t
ORIGIN      
        1 ttttgatacg tatgacccta aaaaagatag ttataaagtt tttgttcagt tcgacgaacg
       61 tattgaaaca gatggagatg ataaaattga acatcgtcaa acatctgcac aattggattt
      121 agttcgtaca gctgaaggtt ggcgtattga taatttaaaa cgtttcaatt taaaacctct
      181 aggacgttag tttgtttcaa gacgatagca atgatataat ttacgtaaaa aggagtgaat
      241 ttatcatgag gcgatggttt gtattaattt taggattagt tatattatta tctgcatgtg
      301 gtcagaaata tgataaagaa attgatgaag ttacaaagtt agaaaaagaa agtattcaag
      361 atgtgaaaaa tacaaagaag tataaaaatg ttgagcggtc aaaaagttat tataagattt
      421 ataatgatgg tgaggttatt ataatgactt atatgccttt taaagatagt aatacaaaag
      481 tttcaagagt atataaaata aatcagacta gcgataaata tgaagaagat tctaatattg
      541 atgctgaaaa gtttgagaaa gataataaac cagtttatga agaaaataat atgaaaaaat
      601 aaagaatcaa ctccctatac gctttagcgg tggtatcggg agttgatgaa catatatcct
      661 cttataactt attttataaa taaccttttt aaaagtaaag gaggatatac atgaaaaaaa
      721 gaaaaaaacg taaagaatta gactataaaa cacgtctagc tatcgcaaag tatttaaatg
      781 atggtttaaa catcattcag atttctaaga ttttaggtcg tgatgatgca gttctctata
      841 gagaagtaaa taagcgttct gttgatggtg tttataatcc tttattggct gaagaagatg
      901 ctataaaatc gcgtagtatt tcagatgaac aggttgctaa acgtagacgt ttgaatgatg
      961 aaacaaaagc atatattgaa gaaaaacttt cattaaaatg gtctcctaga cagatagcaa
     1021 gtcaaattga aaaagataca gggtattata taagttatcc tactatatat agatatattc
     1081 gcaatggatt ggtaaaagtt gatgttaaac gtgatatgag acaagcaggc aagaaatata
     1141 ataagtcttc tgaaaagcgt ggcaaattag acgtagggtc tcgtgttata aagtatcggc
     1201 tcaaaaagta ttaataatcg tgaagaattt ggacattatg aaattgatac tgtttggagt
     1261 gttagaccat caacatattg cttgttaact attattgaaa gaaagacaag atatttatat
     1321 gctagtagat taaatacacg taaatccaat gtagtttgta atgagattat aaatattatg
     1381 aaaccattat tacccaaaag tataactatg gatagaggta aggagtttgc tctatttgaa
     1441 caaatagaag atgaattgaa ttgtagtatt tattttagtg atttgggttg tccatatcaa
     1501 cgtggttcta ttgaaaatgc aaatggactt ttaagacagt attatcctaa aggtacggat
     1561 tttgcaaata taacacaaaa gtcattagat gaagctgtta aacaaattaa tgcacggcct
     1621 cgaatgatat ttgactataa atcgagtgag gaaatgctaa agtaccatgt aagcacacaa
     1681 aattgtgaac ctatattaaa tgactgtgtg aggcacgaac ccgtcaatta atataggtta
     1741 atctaagcga atggtttaca atgagcgcgg aaggacagga cgaataaatt ttgtatataa
     1801 aatgcataat ctaaaaagaa gcatactatt taaatatgtg gtttttgata gatattacta
     1861 gtaaaatgct tctttttctt cttctgtact cttttagtaa tatttattaa aaaaatttaa
     1921 gaaattctca tttttctatt gacacactgc cttaaaagaa agaaaaccag ttgtcagatg
     1981 tttaccatta attatatctt gtttaaaatt aatattaatt ctactatttt tactttcaat
     2041 acttttggtc atatatcaaa ctcaatctgc acaaatctta tttgttttta tagattttct
     2101 aaattttgtc gcgtt
//
LOCUS       DAHHOJ010000195         2087 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.198, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000195 DAHHOJ010000000
VERSION     DAHHOJ010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2087)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2087)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2087
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.198"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..559)
                     /locus_tag="NBH32_002646"
     CDS             complement(<1..559)
                     /locus_tag="NBH32_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCD1516182.1"
                     /translation="MSQNTNHSYFQQNQHAQSISKVWLYFMYYWIIFGIGCYLGQFLP
                     LSWRQPLSFGLLIIILATLVFERARRFGLIISHIYAVVIGLLSYATFTTYLQNLGPDI
                     FYKNIALAIFAFIAFGIIGYFFVGDASSIGKYLFVTLITLIIASLIGIFLHNPIFYTV
                     ITVVSLLLFLLYTLYDFNRLKRGDYS"
     gene            920..1717
                     /locus_tag="NBH32_002647"
     CDS             920..1717
                     /locus_tag="NBH32_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HCD1516183.1"
                     /translation="MKKLAFAITATSGAAAFLTHHDAQASTQHTVQSGESLWSIAQKY
                     NTSVESIKQNNQLDNNLVFPGQVISVGGSDAQNTSNTSPQAGSASSHTVQAGESLNII
                     ASRYGVSVDQLMAANNLRGYLIMPNQTLQIPNGGSGGTTPTATTGSNGNASSFNHQNL
                     YTAGQCTWYVFDRRAQAGSPISTYWSDAKYWAGNAANDGYQVNNTPSVGSIMQSTPGP
                     YGHVAYVERVNGDGSILISEMNYTYGPYNMNYRTIPASEVSSYAFIH"
BASE COUNT          783 a          345 c          294 g          665 t
ORIGIN      
        1 gtgaatagtc acctcttttt aaacgattaa aatcatacaa agtataaagt agaaataaca
       61 acagactaac gacggtaata acagtataaa aaataggatt atgcagaaaa ataccaatta
      121 gactcgcaat aattaaagtt attaatgtaa cgaataaata cttacctata cttgatgcat
      181 ctccaacgaa gaaataacca ataataccaa atgctataaa tgcaaaaatt gctaatgcga
      241 tatttttata gaaaatatct ggtcctaaat tttgtaaata cgtggtaaac gttgcgtatg
      301 acaataagcc aattactaca gcgtaaatat gtgaaatgat taaaccgaac cgtctcgctc
      361 tctcaaatac aagtgttgct aaaataataa tcagtaatcc aaatgacaag ggttgtcgcc
      421 aacttaatgg taaaaactga cctagatagc atcctatgcc aaatataatc cagtaataca
      481 taaaataaag ccacacttta cttattgatt gagcatgctg gttttgctga aaatatgaat
      541 gatttgtatt ttgcgacaaa ataattcctc cttctactgt cgttttatgc gtcaaatttt
      601 acagtaaatt ttgcttcatc aaaagaaata acctttaaca agtacagtaa ttttacattt
      661 tacaatgtta caaaatataa atttttataa ttagttaaaa tcactaaaac gcttttatac
      721 actatcaaat cagcatttat aaaaatatga accgatatcc taaaatgtta ataatattac
      781 aagataataa caaaccacac aaagctactt atttttgata atatggaaag cgtaatataa
      841 aacgaaaact taatttacta tataaattgt cttaataatt tttaaaagta gtaaatctta
      901 attttaagga ggagtccctt tgaaaaaatt agcatttgca ataacagcaa catctggtgc
      961 agctgcattt ttaacgcatc atgatgcaca agcttctaca caacatacag tacaatctgg
     1021 tgaatcatta tggagtattg ctcaaaaata caacacttca gtagagagta ttaaacaaaa
     1081 taaccaatta gataacaact tggtattccc tggtcaagtt atctcagtag gtggaagtga
     1141 tgcacaaaat acgtcaaaca cttctccaca agctggttca gcatcatctc atactgtaca
     1201 agctggtgaa tcattaaata tcattgctag cagatatggt gtttcagttg atcaattaat
     1261 ggcagccaat aacttacgtg gttatttaat tatgcctaac caaacattac aaattcctaa
     1321 tggtggatca ggtggtacaa caccaacagc tacaacaggt agcaatggca atgcatcatc
     1381 ttttaatcac caaaatttat acactgctgg tcaatgtaca tggtacgtgt ttgaccgtcg
     1441 tgctcaagct ggtagtccaa ttagcacata ttggtcagac gctaagtatt gggctggtaa
     1501 cgcagctaat gatggttacc aagtaaacaa cacaccatca gttggttcaa ttatgcaaag
     1561 cacacctggc ccatatggtc atgttgctta tgttgaacgt gtcaatggtg atggtagtat
     1621 cttgatttct gaaatgaatt acacatatgg tccatacaat atgaactacc gtacaattcc
     1681 agcttcagaa gtttctagct atgcattcat ccattaatta aataaattgt actgatatat
     1741 actagcaatt cacatcatgt gagattgcta gttttttatt tttgaaaaaa attttcattt
     1801 tggtacaaaa aattatctca cccttcccta tcatacttat ttatattttg tatgaatggt
     1861 agttaggtaa aaaataataa cctacctatt tgattaaatt aaatgcataa tatattaaaa
     1921 atcaaaataa tgcaactaag ttgactatat aatgatttaa tttgttatac cagtatttta
     1981 cgctttttcg tctacatata caaatttata ttaaataaag tccaatacaa tttaggttaa
     2041 tcaaacaagt tgatagctat ttaattattc attcattgca gaatata
//
LOCUS       DAHHOJ010000196         2024 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.199, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000196 DAHHOJ010000000
VERSION     DAHHOJ010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.199"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(232..330)
                     /locus_tag="NBH32_002648"
                     /pseudo
     CDS             complement(232..330)
                     /locus_tag="NBH32_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000429703.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="head morphogenesis protein"
     gene            complement(306..413)
                     /locus_tag="NBH32_002649"
     CDS             complement(306..413)
                     /locus_tag="NBH32_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516184.1"
                     /translation="MCPKRADDILKASMEYQSTDYKRRYISWKTMIAMK"
     gene            complement(488..1774)
                     /gene="ltrA"
                     /locus_tag="NBH32_002650"
     CDS             complement(488..1774)
                     /gene="ltrA"
                     /locus_tag="NBH32_002650"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020219999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCD1516185.1"
                     /translation="MYRESPSMMELVVREHNIQKAIKKVKKNKGAPGIDGMKVSEIQG
                     HFAQYFPKIKQKLLEGTYKPQAVKKVEIPKANGKKRVLGIPVVRDRVIQQAIKQVIEP
                     SIDRTFSKHSHGFRPNRSTGTALKECASYYEAGYTIAVDCDLKQCFDNINHDKLMYLF
                     ERHIKDKAVSTFIRRRLQVGAIDLSGEVAERKIGAPQGGVISPLLCNIYLHELDKELE
                     KRNHRFVRYADDFVIFVKTKRAGERVMDSIKTFIHKTLKLEVNNDKSKVGSPTRLKFL
                     SCLMSKVNGTYRFRPTTEAKRNLKRNLKWLTRRSRPGSFQDIITEINRVTRGWINYFG
                     KGFIKRFLKELEPWLNRRIRQLILKRWKKIKTKYKMLRKYGLDHDSAMRIASSRKKYW
                     RLSSTHEVHRALTTKRLRKWGLLSLTQLAETAYARY"
BASE COUNT          522 a          417 c          337 g          748 t
ORIGIN      
        1 aatatgcgta aaatatttag cacaaaattt actcactatt ttaccatgaa acctatttga
       61 cttggtaata aattttactt gtcccttatt agtaacgatt gtcattgatt ttattgatgg
      121 atgcttaaaa aatgtaaata aatcatattc tgaaaatcct gactgtccag gatggttatg
      181 taacataaca attgaattcg gtttactgtt aaataataat tcggttgctt gttaccctgg
      241 cacaaaagat acactatctt gattgacata tacttttgta aatttaccat cttttaacaa
      301 ataagctact tcattgctat cattgttttc catgagatat acctccgttt atagtctgtc
      361 gactgatatt ccatacttgc ttttaaaatg tcatcagcac gttttggaca aatcgagtag
      421 gagaataatc attacttgat tattcgtcct ctcacaccac cgagcgtacg gttccgtact
      481 cggcggttca atatcttgcg taagccgtct ctgcgagttg ggttagtgat aataaccccc
      541 acttgcggag acgttttgtt gtaagtgcac gatgaacctc atgcgtagat gataaacgcc
      601 agtatttctt tcttgagctc gcgattctca ttgcgctatc gtggtctaga ccatacttgc
      661 gcaacatctt atattttgtt ttaatcttct tccatctttt gagtatgagt tgcctaatgc
      721 ggcgatttag ccaaggttct aattctttaa gaaatctttt tatgaatcct tttccaaaat
      781 agttaatcca tcctcgtgtc acacgattaa tttctgttat aatatcttgg aatgaacctg
      841 gtctacttcg tctggttaac cactttaagt tgcgttttaa atttcttttt gcttccgtag
      901 tgggtctgaa acgataagtg ccatttactt tgctcattag acaactcaag aactttaaac
      961 gggtaggaga acccacctta cttttatcat tattgacttc aagtttgagg gttttgtgta
     1021 tgaatgtttt aatactatcc atcacacgtt cacctgcacg tttcgtcttg acaaagatga
     1081 cgaagtcatc tgcatatctc acaaaacgat gattacgttt ttcgagctct ttatccagtt
     1141 catgtagata tatgttacag agtaatggtg atatcacacc accttgcggt gcacctatct
     1201 ttctttctgc gacttcgcca gataggtcaa tggcaccaac ttgtaacctt ctacggataa
     1261 atgtcgatac cgctttatct ttaatgtggc gctcaaataa atacatgagt ttatcatgat
     1321 ttatgttgtc aaagcattgt tttaaatcac aatctacggc tatcgtgtaa cctgcttcat
     1381 agtaagacgc acattctttc agtgctgttc ctgtactacg attaggtcta aagccatgac
     1441 tgtgtttaga gaaggtacga tcgatactag gttcaatcac ttgtttaatt gcttgttgta
     1501 tcactctgtc tcttacgaca ggtataccaa gcacacgctt tttcccattt gctttgggaa
     1561 tttcaacttt cttaactgct tgaggtttgt atgtaccttc aagcagtttt tgttttattt
     1621 ttggaaagta ttgcgcaaag tgaccttgta tttcactcac tttcatgcca tcaatgccag
     1681 gggcaccttt attcttttta actttcttaa ttgccttttg aatgttgtgt tctcttacaa
     1741 caagctccat catcgatgga gactcacgat acatttcttt catttcacct aagatttacc
     1801 atgcgcactt atttaccctt tttcgttcca ctacttatct ctctatatgt tgccaactag
     1861 ttcgttgttt tctgcatgat gtaaatctat aacctccatt ctttactttg tatgaatatt
     1921 gttccgtcct tcggtacatt ttccctacta tgacgtctgc tgacttctca ttattcgttg
     1981 ttactacggt atgtgcaccg ctaatgagac ctccctgggt aagg
//
LOCUS       DAHHOJ010000197         2019 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.200, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000197 DAHHOJ010000000
VERSION     DAHHOJ010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.200"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..525
                     /locus_tag="NBH32_002651"
     CDS             <1..525
                     /locus_tag="NBH32_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000776424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HCD1516186.1"
                     /translation="IVSMLAIIMKLIYNKLNMLNKINFVMKLPLISGYFQLFKTYFVT
                     NELVLFYKNGITLQSIVDVYINHSSDPFRQFLGKYLLTYSEMGYGLPQILEKLKCFKP
                     QLIKFVLQGEKRGKLEVELKLYSQILVKQIEDKAIKQTQFLQPILFLILGLFIVAIYL
                     VIMLPMFQMMQSIK"
     gene            539..850
                     /locus_tag="NBH32_002652"
     CDS             539..850
                     /locus_tag="NBH32_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD1516187.1"
                     /translation="MFKFLKKTQAFTLIEMLLVLLIISLLLILIIPNIAKQTAHIQST
                     GCNAQVKMVNSQIEAYALKHNRNPSSIEDLIADGFIKEAQKTCKSGETITISNGEAVA
                     N"
     gene            828..1274
                     /locus_tag="NBH32_002653"
     CDS             828..1274
                     /locus_tag="NBH32_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HCD1516188.1"
                     /translation="MEKQLQIRKQSAFTMIEILVVMMLISIFLLLTMTSKGLSNLRVI
                     DDEANIISLITELNYIKSQAIANQGYINVRFYENSDTIKVIENNKIRFLKLKVGKIIN
                     VAKVDIIAFDKKGNINKFGSITIDNNNSIYRIIFHIEKGRIRYEKL"
     gene            1261..1560
                     /locus_tag="NBH32_002654"
     CDS             1261..1560
                     /locus_tag="NBH32_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000844410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGE"
                     /protein_id="HCD1516189.1"
                     /translation="MKSYKCKGSFLIDSMAGFLLIGLITLLLIPMMNQMQASINHKLQ
                     TIDASKVILTTVSKINKEELKKGVTIGKYDIKQSDQQICAISKNTTSYQKTCIQY"
     gene            1478..1975
                     /locus_tag="NBH32_002655"
     CDS             1478..1975
                     /locus_tag="NBH32_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCD1516190.1"
                     /translation="MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVI
                     SITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDILEDFRGVDRNDIEIRQQRIILH
                     KGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
                     KTIYV"
BASE COUNT          814 a          225 c          314 g          666 t
ORIGIN      
        1 atagtatcta tgttggctat tattatgaaa ttaatttata acaaattaaa tatgctcaat
       61 aagataaact ttgtgatgaa actaccgcta atatcgggct atttccaatt atttaaaact
      121 tattttgtaa ctaatgaatt agtgttattt tataaaaatg gtattacact tcaatcaata
      181 gtagacgttt atattaacca tagtagtgat ccatttagac agtttctagg taaatactta
      241 ttaacttatt cagaaatggg atatggttta cctcaaattt tagaaaaact aaaatgcttt
      301 aagcctcaat taattaagtt tgtgctacaa ggtgaaaaga gaggaaagct agaagtagaa
      361 ctaaagttat attcgcaaat attagtaaaa caaatagaag ataaagcgat aaaacagact
      421 cagtttttac agcctatttt atttttgatt ttaggtttat ttattgtcgc aatttatttg
      481 gtaattatgt taccaatgtt tcaaatgatg caaagtataa aataatggag gttaatatat
      541 gtttaaattt cttaagaaaa ctcaagcgtt tacattgata gagatgctat tagtgttatt
      601 aatcatcagt ttattattaa ttttaatcat tccaaatatt gctaaacaaa ctgctcacat
      661 acaatcaaca ggttgtaatg cacaggtaaa aatggttaat agtcaaattg aagcgtatgc
      721 attgaaacat aatagaaatc catcgtctat tgaagactta attgcagatg gttttataaa
      781 agaagcacaa aagacatgta aatcaggaga gacaatcaca attagtaatg gagaagcagt
      841 tgcaaattag aaagcagtca gcatttacta tgattgagat acttgtggta atgatgttaa
      901 tcagtatatt tttacttttg acaatgacat ctaaaggatt aagcaatctt agagtaatag
      961 atgacgaggc aaatatcatt tctcttatta ctgaattgaa ttatattaaa tcgcaagcta
     1021 tagcaaatca aggatatatc aatgttagat tttatgaaaa cagtgacact attaaagtaa
     1081 tagagaataa taaaatacga tttctaaaat taaaagtagg caaaataatt aatgttgcaa
     1141 aagttgatat tattgccttt gataaaaaag ggaatatcaa taaatttggt agcataacaa
     1201 ttgacaataa caattcaatt tatagaataa tattccatat tgaaaaagga agaattcgtt
     1261 atgaaaagct ataagtgtaa aggatcattc ttaatagata gtatggctgg atttttgcta
     1321 attggattga ttacattact attgatacca atgatgaatc aaatgcaagc gagtataaac
     1381 cataaactac aaacaattga tgcttctaaa gtaattttga cgactgtatc taaaattaat
     1441 aaagaagaac ttaagaaggg ggtaactata gggaaatatg atattaagca aagtgaccaa
     1501 caaatttgtg ctatttcaaa aaataccact tcttatcaaa agacatgtat acagtattaa
     1561 tgtcaaagct ttttcgctta ttgaaatgtt agtagcgatg atggttataa gtataacttt
     1621 actaattgtt ccagacttaa ttagacttag caaaactttt ctaattgaaa gtagggaatt
     1681 aacaactgta gatttcgaat ttttctcaag agatattcta gaggatttta gaggtgtaga
     1741 tagaaacgat attgaaatta ggcaacagcg tatcatttta cataaaggtg aagaaatgat
     1801 cgaatacaaa ttaataaata ataaaattat taaagttgta aatgacagag gaaatataac
     1861 aatgattaat aatgttacag catttactgc aaatatctac tataaatcta ttattaaaat
     1921 aacgataaca gttaaagtcg gtacaaatgt gcagactaaa actatttatg tataacttac
     1981 tagaatttaa atgagtacta ttaaaaagga tgtgaaata
//
LOCUS       DAHHOJ010000198         1958 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.201, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000198 DAHHOJ010000000
VERSION     DAHHOJ010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1958)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1958)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1958
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.201"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(294..1940)
                     /locus_tag="NBH32_002656"
     CDS             complement(294..1940)
                     /locus_tag="NBH32_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BCCT family transporter"
                     /protein_id="HCD1516191.1"
                     /translation="MNSSSPENPNGKKYSPVFIYSAIVVAIVVLLGAFLPEQFNYVTN
                     NIKMWITEKLGWYYLILTTIIVFFCIFLIFSPIGKLKLGKPNDKPEFNTISWFAMLFS
                     AGMGIGLVFYGAAEPMAHFATPPTADPKTTEAYTEALRSTFFHWGFHAWAVYGVVALA
                     LAYSQFRKGEPGLLSRTLRPLLGDKVEGPIGIFIDVLSVFATIVGVAVSLGMGALQIN
                     GGLHYLFNVPNNTFVQAIIIIVVTILFIASAWSGLSKGIQYLSNLNIGLGTILMVAAL
                     IVGPTVLILNMLTSSTGSLLNTFLFNSFDTAALNPQKREWMSSWTLYYWGWWLSWSPF
                     VGVFIARVSKGRSIREFISGVLLVPAIVSFVWFSVFGVLGIETGKKHKEIFDMTPETQ
                     LFGVFNHVPFGIVLSLIALLLIASFFITSADSATFVLGMQTTFGSLNPSSMVKVVWGI
                     SQALIAFVLLLAGGGNGAEALNAIQSAAIISAFPFSFVVILMMVSFYKDANQERKFLG
                     LTLTPNKHRLQEYIKSQQEDYESDILEKRQSRRNIEKKDN"
BASE COUNT          704 a          381 c          291 g          582 t
ORIGIN      
        1 atagaatatt gatttatgtc ccagcctgag ttaatttttt ataaaagtat agttaatttg
       61 cgtttatacc gtcaaacttc actttagctt tgtcaaaccc ctttctatta agttgtcaga
      121 aataaaccta tcttaaaata taaaaaaatc gagaattcgt agtttaataa cgaaattctc
      181 gttcttatcc ttttgaatat actcaatttt ccacaaaaac aaacaagtag tatatctgtt
      241 ctagctacta gaatgacata ctacttgtta tttaaatact taactaaatt ttattagtta
      301 tcttttttct ctatatttct acgtgactga cgcttttcaa gaatgtcaga ttcataatct
      361 tcttgttgac tcttgatata ttcttgtaag cgatgtttat tcggagtcaa tgttaaacct
      421 aggaatttac gttcctggtt cgcatccttg tagaaactta ccatcatgag tatgacgaca
      481 aaggagaatg ggaatgcact tataattgca gcactttgaa tcgcatttaa agcttcagcg
      541 ccgttaccgc caccagctaa taaaagtaca aatgctatta aggcctgtga aattccccaa
      601 acaactttta ccatactaga tggatttaat gaaccaaatg ttgtttgcat tcctaataca
      661 aatgttgctg agtcagcaga tgtaataaag aatgatgcaa ttaataataa tgcaatcaac
      721 gataaaacaa tgccaaatgg cacatgatta aacactccaa atagctgtgt ttcaggagtc
      781 atatcaaaaa tttctttgtg tttcttacct gtctcgatgc ctaatacacc aaagacacta
      841 aaccaaacaa aactaacaat tgctggaact agcaagacac cagaaatgaa ctctctaatt
      901 gaacgtcctt ttgaaactcg tgcaataaac actccaacga atgggctcca acttaaccac
      961 caaccccagt aataaagtgt ccatgaagac atccattcac gtttttgagg atttaaagct
     1021 gctgtatcaa aactattaaa caagaatgtg tttagtaaac tacccgtaga gctagttaac
     1081 atatttaaaa taagaacagt tggtccaaca attaaagcag ctaccattaa aatagtacct
     1141 aaaccaatgt tcaagttact taagtattga atacctttac ttaatccaga ccatgcactt
     1201 gctataaata agatagtaac aacaatgatg ataatcgctt gtacaaacgt attgtttgga
     1261 acattgaaca agtaatgtaa accaccatta atttgtagag cacccatacc taacgaaacg
     1321 gctaccccaa cgattgtcgc aaatacagat aaaacgtcaa taaaaatccc aataggacct
     1381 tctactttat cacctaaaag aggacgtaaa gttctagata ataaacctgg ttcaccttta
     1441 cggaattgcg aatatgccaa cgctaacgca acaacaccat aaacagccca agcatggaat
     1501 ccccaatgga aaaatgttga acgtagagct tcagtataag cttcagtagt tttgggatct
     1561 gctgtaggtg gcgtagcaaa gtgcgccatc ggttcagctg caccataaaa caccaaacct
     1621 atccccatac cagcactaaa caacatagca aaccatgaaa ttgtattaaa ctcaggtttg
     1681 tcatttggtt tacctagttt aagttttcca ataggactaa aaataaggaa tatacagaag
     1741 aaaacgataa tcgtagtaag aataagataa taccaaccta acttttctgt aatccacatt
     1801 ttaatattat tggtaacata gttgaattgt tcaggtaaaa atgcaccaag taatacgact
     1861 atagcaacaa caattgcact atagatgaag actggtgaat acttctttcc atttggattc
     1921 tctggtgaag aagaattcat aattaattac tcccttca
//
LOCUS       DAHHOJ010000199         1924 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.203, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000199 DAHHOJ010000000
VERSION     DAHHOJ010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1924)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1924)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1924
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.203"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..664
                     /locus_tag="NBH32_002657"
     CDS             <1..664
                     /locus_tag="NBH32_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-type lipoprotein"
                     /protein_id="HCD1516192.1"
                     /translation="KLYPTKNLDDFYDKEGFRDQEFKKGDKGTWIVNSEMVIEPKGKD
                     METRGMVLYINRNTRTTKGYYFISEMTDDSNGRPKDDEKRYPVKMEHNKIIPTKPLPN
                     DKLRKEIENFKFFVQYGDFKDINDYKDGDISYNPNVPSYSAKYQLKNDDYNVKQLRKR
                     YDIPTNKAPKLLIKGDGDLKGSSVGSKNLEFTFVENKEENIYFTDSVQYTPSEGTSYE
                     SN"
     gene            complement(668..790)
                     /locus_tag="NBH32_002658"
     CDS             complement(668..790)
                     /locus_tag="NBH32_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516193.1"
                     /translation="MSYIIKFTFKFFEMATYISQLTLDIELVSSELLVILPGFI"
     gene            1225..1416
                     /locus_tag="NBH32_002659"
     CDS             1225..1416
                     /locus_tag="NBH32_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516194.1"
                     /translation="MRKEIEALIFSDVSSYDIYVNTGVNQGLIGDIKDGYLTIDSMPY
                     IDAERLYHFAMERKSLVTN"
     gene            1716..>1924
                     /locus_tag="NBH32_002660"
     CDS             1716..>1924
                     /locus_tag="NBH32_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCD1516195.1"
                     /translation="MKLSLDINTDFEVTTLTDLPKLKIVMENLNMKINKSEIARHMGV
                     DRRTVDKYLNGFEPTKKRNRQSIID"
BASE COUNT          751 a          267 c          329 g          577 t
ORIGIN      
        1 aaaattatat ccaaccaaaa atctcgatga tttttacgat aaagaaggct ttcgagatca
       61 agaatttaaa aagggtgata aaggtacttg gatagttaat tctgaaatgg taatcgagcc
      121 aaaaggtaag gatatggaaa cgagaggaat ggtgctctat atcaatcgca atactagaac
      181 cacaaaaggg tattatttta taagtgaaat gacagatgac agtaacggca ggccaaagga
      241 tgatgaaaaa aggtatccgg taaaaatgga acataataaa atcataccaa cgaagccact
      301 accgaatgac aagttaagaa aagaaatcga aaactttaaa ttctttgtac aatacggtga
      361 ctttaaagat attaatgatt ataaagatgg tgacatttca tataatccta atgttccaag
      421 ttattccgca aaatatcaat tgaaaaacga tgactacaat gtgaagcaac ttcgaaaaag
      481 atatgatatt ccaaccaaca aagctccgaa attattaata aaaggtgatg gtgatttaaa
      541 aggctcatct gtaggatcaa aaaatctaga atttaccttt gtagaaaata aagaagaaaa
      601 catttatttt acagattctg tacaatatac tccaagtgag ggtacaagtt atgagtcaaa
      661 ctgaatatca aataaaacct ggtaatataa caagtaactc tgaagaaaca agttcgatat
      721 ctaaagtgag ctgtgaaata taggtagcca tttcaaaaaa tttaaaggtg aatttgataa
      781 tgtagctcaa ggagattggg ttaaaaaggc gaagaatgaa ctggatgata ttagtaagaa
      841 ataaaaaaat attcaaagaa cggaagttta atattctagc cgattcctga aactaagaca
      901 gcttgtgttc attcatgcca ttatcaatat aatcactaca acaaaaaagc ttctaatgtg
      961 atacaggaac ctcatattcc gtatcatgtt agaagctttt aatgtctaaa gaacatctac
     1021 attttatcat attttcttac ttattaaact tttatataat taaatatttc ttaattttcc
     1081 aaaatagtga taaatttgtg aaatacatca caaatccctt tatttatttg gaaattcatg
     1141 taatattaga cttgtaagaa gttaataaat agagagagac gagagagttt atataaataa
     1201 tatataaaca ttggagtgat gattatgaga aaagagattg aagcgcttat tttctcagac
     1261 gtaagtagct atgatattta cgtgaacact ggtgtaaatc aaggattaat tggtgacatc
     1321 aaagatggtt acctaactat tgattctatg ccttacattg atgctgagcg tttgtatcac
     1381 tttgctatgg aacgtaaatc gttagtcact aactagttct tattgccaat gattactacc
     1441 cctagtcggc ggcaattgaa gtgtgattga tgtaacttgc cctcgttggt gagcaattga
     1501 gggcagaccc ctttaattaa gtaaacccta actccccaca aatctggaac gatactaaaa
     1561 gccacgtcct atattggatg tggctttagt catgcttata ttatttttaa aacgattacc
     1621 tactggaaat gataatttaa taatgtacac ttttgattgt ttaaacatgt acaattttta
     1681 aacacctaga gataatttta ttgaggtgaa caacaatgaa attatcttta gacataaata
     1741 ctgattttga agttactact cttacagatt taccaaaatt aaaaattgtt atggagaact
     1801 taaacatgaa aataaacaaa agtgaaatag cgagacacat gggtgttgat agaagaacag
     1861 ttgataaata tttaaatggc tttgagccaa ccaaaaagag aaatcgtcag tctattattg
     1921 acaa
//
LOCUS       DAHHOJ010000200         1910 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.204, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000200 DAHHOJ010000000
VERSION     DAHHOJ010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1910)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1910)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1910
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.204"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(168..1175)
                     /locus_tag="NBH32_002661"
     CDS             complement(168..1175)
                     /locus_tag="NBH32_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCD1516196.1"
                     /translation="MSYIIIVTIAVVIFSLIFDFINGFHDTANAVATAVSTRALTPKT
                     AILMAAVMNFIGALTFTGVAGTITKDIVDPFKLENGLVVVLAAILAAIVWNLATWFYG
                     IPSSSSHALIGSIAGAAIASEGSFGVLHYQGFTKIIIVLIVSPIIAFCVGFLMYSIFK
                     VIFKNANLTRANRNFRFFQIFTAALQSFSHGTNDAQKSMGIITLALIVANVQNDGSVE
                     PQLWVKFACATAMGLGTAIGGWKIIKTVGGNIMKIRPANGAAADLSSALTIFVASSLH
                     FPLSTTHVVSSSILGVGASNRAKGVKWSTAQRMIITWVITLPISALLAGLLFYILNLF
                     F"
     gene            complement(1191..1808)
                     /locus_tag="NBH32_002662"
     CDS             complement(1191..1808)
                     /locus_tag="NBH32_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 domain-containing protein"
                     /protein_id="HCD1516197.1"
                     /translation="MFSKKKDKFMVQLEEMVFNLDRAAIEFGKMDFNTHLDLKAYSDN
                     IKTYESHGDELVHQVITDLNQTFITPIEREDILSLCDAIDDVLDAIEETAAMFEMYSI
                     EYTDEYMAEFVDNIQKAVAEMKLAVGLLVDKKLSHMRIHSINIKEFETNCDGILRQSI
                     KHIFNSETDPITLIKIKDIYESMEEIADKCQIVANNFETIIMKNS"
BASE COUNT          647 a          346 c          278 g          639 t
ORIGIN      
        1 gtttatgttc catggaatac atttataact tagcccctaa ttaaaatgct ttgtcttggt
       61 cattctatgt aaattctata aaatatgtta tctatttaca taaaaaactg aacttcaata
      121 ccaccacatt tttgtgatac tgaagttcag tttagtttta ttttcaatta gaaaaataag
      181 ttaagtatat agaatagtaa acctgctaac aatgctgaaa taggtaatgt aatcacccat
      241 gtaatgatca ttcgttgcgc agtactccat tttacacctt tagctcggtt agaagcacca
      301 acacctaaga ttgatgatga cacaacgtga gttgttgata atgggaaatg tagcgatgat
      361 gcaacaaaaa ttgttaatgc agatgataaa tcggccgcag caccatttgc tggacgtatt
      421 ttcataatat taccacctac agttttgata attttccagc caccaattgc agtaccaagc
      481 cccattgctg tcgcacaggc aaattttacc cataactgtg gttcaacgct gccatcattc
      541 tgtacattag caacaatcaa tgccaacgta ataataccca ttgatttttg cgcatcattc
      601 gtaccgtgag agaatgattg taacgctgct gtgaaaattt ggaaaaatct aaagttacga
      661 ttcgctcttg ttaaatttgc atttttaaag ataactttaa aaattgaata catcaagaaa
      721 ccaacacaaa atgcgataat cggtgaaacg attaatacaa taataatttt tgtgaaacct
      781 tggtaatgta acactccaaa tgagccttca gatgcgattg ctgctcccgc aattgaacct
      841 ataagtgcat gtgaagacga acttggaatt ccgtaaaacc aagtagctaa attccaaaca
      901 atagccgcaa gtattgcagc taacacaaca actaagccat tttccaattt aaatggatcg
      961 acaatgtctt tagtaatggt gcctgcaacg cccgtaaatg ttaaagcacc tataaagttc
     1021 atcactgctg ccattaaaat tgccgtttta ggcgttaacg ctctagtaga tacagcagta
     1081 gctactgcat tggctgtatc atggaatcca ttgataaagt caaatatcag cgagaaaata
     1141 actacagcta tagtgacgat gattatatat gacataaata taaactcccc ttagctattt
     1201 ttcataataa tagtttcaaa attatttgct acgatttgac atttatcagc gatttcttcc
     1261 atgctttcat aaatatcttt tattttaatt aaagtgattg gatctgtttc gctattgaaa
     1321 atatgtttaa ttgactgtct taaaatacca tcacagtttg tttcaaattc tttaatatta
     1381 attgaatgaa tacgcatatg tgataatttt ttatcgacta ataagccgac agcaagtttc
     1441 atttctgcaa ctgctttttg aatgttatca acaaactcag ccatatattc atctgtgtat
     1501 tcgattgaat acatttcaaa catagctgcc gtttcttcaa ttgcatctaa aacatcatca
     1561 attgcatcac ataatgataa aatatcttca cgttcaattg gtgtgataaa tgtttgattt
     1621 aaatcagtaa ttacttgatg tactaattcg tcaccatgtg actcataagt tttaatgttg
     1681 tctgagtatg cttttaaatc taaatgagta ttgaaatcca ttttaccgaa ttcaatagca
     1741 gcacgatcca gattgaaaac catctcttct aattgaacca taaacttatc ttttttctta
     1801 ctaaacattt aaaatcctcc atttaagcga ttgtcaccaa tcacattcag ttataatttg
     1861 tttcaaatta agacaagtga atttacaaac taatgataca aatttgttat
//
LOCUS       DAHHOJ010000201         1871 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.205, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000201 DAHHOJ010000000
VERSION     DAHHOJ010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.205"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            18..278
                     /locus_tag="NBH32_002663"
     CDS             18..278
                     /locus_tag="NBH32_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516198.1"
                     /translation="MNNILLNAINIVITTTFVIFNILITYNKDLDDLCWLLPGIIICG
                     VILIVSFTIAMITKNWLSEILFFINIVLVLYYIYPIFYSFIG"
     gene            307..495
                     /locus_tag="NBH32_002664"
     CDS             307..495
                     /locus_tag="NBH32_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516199.1"
                     /translation="MSLHFAILFWLALIFLVAATFILVLMKKTGKESKKESYLSFTVI
                     LYIFGFAILIYTFIFGVL"
     gene            683..1252
                     /locus_tag="NBH32_002665"
     CDS             683..1252
                     /locus_tag="NBH32_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516200.1"
                     /translation="MNYIKRTIILLILFVVVSPINSPKTIADNKYSEIQDDKFQLKPG
                     DIIVTKGPVMWGFFGHCSIAIDDKTILQIEGPGDKPTTQSFESFKYNYASGKNDWMKV
                     YRCSYPGAGKKAAEWVKKNYENTNHRYLVTLNLNSKKFTYCTKIIYQAYKFGVSEKSV
                     KSYGLHIISPYAIKDNFIDPYKLRLVKAY"
     gene            1340..1624
                     /locus_tag="NBH32_002666"
     CDS             1340..1624
                     /locus_tag="NBH32_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516201.1"
                     /translation="MNELTKEQKYTIAKFYKLYIERSNKGETETVANFFGDAKDAREN
                     YFCDRDYQDFLTNCQILIQNKYLTGEVLDDNIYNISILNKTFIEFEQNFG"
     gene            1686..>1871
                     /locus_tag="NBH32_002667"
     CDS             1686..>1871
                     /locus_tag="NBH32_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516202.1"
                     /translation="MSEWHINNQSTKPFLIQQAEEQLSIVKPLPNGSFQILTEIDLEN
                     GNVNNIDHSLLVSVDLKA"
BASE COUNT          659 a          271 c          275 g          666 t
ORIGIN      
        1 actagtaggt gtcagtcatg aataacattt tgttaaatgc tatcaatata gttattacta
       61 ccacttttgt tatctttaat attttaatca catataataa agatttagat gatttatgtt
      121 ggctcctgcc tggtattatc atttgtggtg tgatactcat cgtatccttt accattgcaa
      181 tgataactaa aaactggtta agtgaaatat tattttttat aaatatcgta ctcgttctct
      241 attacattta tcctattttt tatagtttta taggttaata attacggtat ataaggaggg
      301 tttcacatgt ctttacattt tgcaattctg ttttggctag cattaatttt cttagttgcc
      361 gctacgttta tactcgtatt aatgaaaaaa actggcaaag aatctaaaaa agagtcctat
      421 ttaagtttta ctgtcattct ctatattttt ggattcgcta tattaatata cacatttata
      481 tttggtgtgc tataaatgaa aatcatttta tatccagtct gaaacgttcc aatgatgttt
      541 cagactgttt tttattctta atagacattt cacactctca tactttaata tttaagatat
      601 gttttagtct taaatgctta ttaaagatta caattctaat ttaagactat tcacatatat
      661 ttcttcagca aggagctttt aagtgaacta tattaaacga acgataattt tacttatatt
      721 attcgttgtc gtatccccta taaactcacc aaaaacaatt gcagataata agtattcaga
      781 aattcaagat gacaaattcc aattaaaacc tggtgatatt attgttacaa aaggccctgt
      841 catgtgggga ttttttggtc attgtagtat cgcgattgat gataaaacga ttttacaaat
      901 tgaggggcct ggcgacaaac caactacaca atccttcgaa tcttttaaat ataattatgc
      961 aagtggcaaa aatgattgga tgaaagttta tcgttgcagc tatcctggtg caggtaaaaa
     1021 agcggctgaa tgggttaaga aaaactatga aaatacaaat catcgttatc tagttacatt
     1081 aaatttgaac agcaaaaaat ttacgtattg tactaaaatt atctaccaag cttataaatt
     1141 tggtgtaagt gaaaaatcag ttaaaagtta cggactacat attatttcac cctatgcaat
     1201 taaagataat tttatagatc catacaaact aaggcttgtt aaagcttatt aacacagttt
     1261 catcaaatag ttaaattagt ttgtgttttg caataaattg ggtatagatt acaaatgata
     1321 attcaggagg cccaatattt tgaatgaact aactaaagaa caaaagtata caatagctaa
     1381 attttacaaa ttatatattg aacgttcgaa taagggagaa acagaaacag tcgctaactt
     1441 ttttggcgat gctaaagatg cacgtgaaaa ctatttttgc gatcgtgatt atcaagactt
     1501 cttaactaac tgccaaattt taattcaaaa caaatactta actggtgaag ttttggacga
     1561 taacatttac aatatttcta ttttaaacaa gacatttatt gaatttgaac aaaattttgg
     1621 ttgagcatga atgtctcaac tataaacttt ttaaccctat attaaagcag gagtgaaaaa
     1681 catacatgag tgaatggcat attaataatc aatctacaaa acctttttta atacaacaag
     1741 cggaggagca attgtctatt gttaaaccat tgcctaatgg tagctttcaa atactcacag
     1801 aaatagattt ggagaatggc aatgttaata acatcgatca ttctctactt gtttctgtag
     1861 acctcaaagc a
//
LOCUS       DAHHOJ010000202         1772 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.207, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000202 DAHHOJ010000000
VERSION     DAHHOJ010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1772)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.207"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(75..470)
                     /gene="spxA"
                     /locus_tag="NBH32_002668"
     CDS             complement(75..470)
                     /gene="spxA"
                     /locus_tag="NBH32_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HCD1516203.1"
                     /translation="MVTLFTSPSCTSCRKAKAWLQEHDIPYTERNIFSEHLTIDEIKQ
                     ILKMTEDGTDEIISTRSKTYQKLNVDIDSLPLQDLYSIIQDNPGLLRRPIILDNKRLQ
                     VGYNDDEIRRFLPRKVRTFQLQEAQRMVD"
     gene            765..1754
                     /gene="trpS"
                     /locus_tag="NBH32_002669"
     CDS             765..1754
                     /gene="trpS"
                     /locus_tag="NBH32_002669"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCD1516204.1"
                     /translation="METLFSGIQPSGIPTIGNYIGALKQFVDVQNDYDCYFCIVDQHA
                     ITMPQDRLKLRKQTRQLAAIYLASGIDPDKATLFIQSEVPAHVQAGWMLTTIASVGEL
                     ERMTQYKDKAQKAVEGIPAGLLTYPPLMAADIVLYNTNIVPVGDDQKQHIELTRNLVD
                     RFNSRYNDVLVKPEIRMPKVGGRVMSLQDPTRKMSKSDDNTKNFISLLDEPNVAAKKI
                     KSAVTDSDGIIKFDRDNKPGITNLISIYAGLTDMPIKDIEAKYEGEGYGKFKGDLAEI
                     VKAFLVEFQEKYESFYNSDKLDDILDQGRDKAHKVSFKTVKKMEKAMGLGRKR"
BASE COUNT          645 a          261 c          296 g          570 t
ORIGIN      
        1 aaaaaagcaa cactatagaa atagtgttgc ttgacatatc ttttagtaaa gtatgacaca
       61 tagttaaatg gttattagtc aaccatacgt tgtgcttctt gtaattggaa cgtacgaact
      121 tttctaggta agaaacgtcg aatctcgtca tcattataac caacttgtag tcgtttatta
      181 tctaaaataa ttggacgacg taataagcca ggattatctt gaatgattga atataagtct
      241 tgtaatggta gtgaatcaat atcaacattt aatttttggt atgttttaga acgtgtagaa
      301 atgatttcat cagtaccgtc ttcagtcatt tttaatattt gcttaatttc atcaattgtt
      361 aaatgttcag aaaaaatatt acgctccgta tacggaatgt catgttcttg taaccatgct
      421 ttcgctttac ggcaagatgt gcaacttggt gaagtaaata atgttaccat acatctcact
      481 ctcctatttg aatgaataaa attcattgct taaaatttag ttatagatca agaaaaaaca
      541 tttttttcta aaattcttaa tcgttactat ttattataac tatctaacat taaaattaaa
      601 tgagaaaaac ctaatttttc agataagttt ctacacttat aaaaaagatt attaatccct
      661 ttgttagtag taagttatac gtatattcta acacatctta catttttaag aaatactgtt
      721 ataatgataa ttattaaaat attactaaga aagtaggcat ttaaatggag acattatttt
      781 caggcatcca acctagtgga attcctacta ttggaaatta tattggcgca ctaaaacaat
      841 ttgttgatgt gcaaaatgac tatgattgtt atttctgtat cgtagatcaa catgcaatta
      901 caatgccaca agatcgctta aaattacgta aacaaactag acaattagca gcgatttatt
      961 tagcttctgg tatagatcca gacaaagcaa cattgttcat acaatctgaa gtccctgcac
     1021 acgtacaagc aggatggatg ttaactacga ttgcttctgt tggagaatta gagcgtatga
     1081 cgcaatacaa agataaagct cagaaagcag ttgaaggtat accagctggt ctattaacat
     1141 atccaccttt aatggcagct gatattgttc tttacaatac taatatcgtt ccagttggcg
     1201 atgaccaaaa gcagcatatc gaattgactc gtaaccttgt agatagattt aatagtcgct
     1261 ataatgatgt gcttgtgaaa cctgaaattc gtatgcctaa agttggtgga cgtgtcatga
     1321 gtttacaaga cccaacaaga aaaatgagta agagtgatga taatactaaa aacttcattt
     1381 cattattaga cgaaccaaat gttgctgcta aaaaaattaa aagtgctgtg actgattcag
     1441 acggtattat taaattcgac cgcgacaata aaccaggtat tacaaattta atttcaatat
     1501 acgctggatt aacagacatg cctattaaag atattgaggc aaaatatgaa ggtgaaggtt
     1561 acggtaaatt taaaggcgac cttgctgaaa tagttaaagc atttttagta gaattccaag
     1621 aaaaatacga aagtttctat aactcagata aacttgatga tattttagat caaggtagag
     1681 ataaagcaca caaagtttca tttaaaactg tcaaaaaaat ggaaaaagcg atgggtttag
     1741 gacgtaagag ataaaataag agcttgggac at
//
LOCUS       DAHHOJ010000203         1759 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.208, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000203 DAHHOJ010000000
VERSION     DAHHOJ010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1759
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.208"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            64..750
                     /locus_tag="NBH32_002670"
     CDS             64..750
                     /locus_tag="NBH32_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain-containing protein"
                     /protein_id="HCD1516205.1"
                     /translation="MDKYQLKARPVVIRRELLDHYSDLGLDEQDLVILLKLIYASETS
                     NKQPSIELLQKGSTMQPRDITMVIQNLIQRELLELQVQKDEEGRFTEYMNLDPFFEKL
                     SHILKQQSMETKEQNSKEKFKQLFRVLEDTFARPLSPYEIETLNQWIDVDKHDTAIIQ
                     AALDEANSLNKLSFKYMDRILLNWKKNNVKTIDDSRKIREKFNKPKMTHTVKTVPKFD
                     WLNGENLDGK"
     gene            740..1399
                     /gene="nth"
                     /locus_tag="NBH32_002671"
     CDS             740..1399
                     /gene="nth"
                     /locus_tag="NBH32_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCD1516206.1"
                     /translation="MVSKKKALEMIDVIANMFPDAECELKHDNPFELTIAVLLSAQCT
                     DVLVNRVTTELFKKYKTPEDYLAVSDEELMNDIRSIGLYRNKAKNIKKLCQSLIDQFN
                     GEIPQTHKELESLAGVGRKTANVVMSVAFDEPSLAVDTHVERVSKRLGINRWKDNVRQ
                     VEDRLCSVIPRDRWNRSHHQLIFFGRYHCLARKPKCDICPLLEDCREGQKRYKASLKE
                     A"
     gene            1404..1745
                     /locus_tag="NBH32_002672"
     CDS             1404..1745
                     /locus_tag="NBH32_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516207.1"
                     /translation="MTNITKEVFDNLEQEIDVFAKNKTLGSSEAKPYLDEYHSKIIDY
                     FKQVNDITGNIDFDNLNQYPVVPMNFKERYDYMIERKYHFMGYRQMKTFKTELIKMNA
                     SYQTRLKNKQV"
BASE COUNT          689 a          220 c          329 g          521 t
ORIGIN      
        1 cgaaaattaa agaaaatgat ttatactatt aagtaatggt taaatttaaa agggggcacg
       61 aacatggata aatatcaatt aaaagcaaga cctgtagtga tacgaagaga attattagac
      121 cattacagtg acttaggttt agatgagcaa gatttagtca ttttgcttaa attgatttat
      181 gcttccgaaa catcaaataa acagccttca attgaattac ttcagaaggg ctcaactatg
      241 caaccacgtg atattacaat ggttattcaa aatttgatac aacgtgaatt gctagaatta
      301 caagttcaaa aagatgaaga aggtagattt accgaatata tgaatctaga tccttttttc
      361 gaaaaattaa gtcatatatt gaagcaacaa agtatggaaa cgaaagagca aaatagtaaa
      421 gaaaaattta aacaattatt tagagtttta gaggatacat ttgcaaggcc gttatctcca
      481 tatgaaatag aaacgttaaa tcaatggatt gatgttgata aacacgatac tgcaattata
      541 caagcggcat tagacgaggc gaatagtttg aataaactta gctttaaata tatggatcgt
      601 attttactta attggaagaa aaacaatgtt aaaacaattg acgattctag aaaaataaga
      661 gaaaaattca ataaaccgaa aatgacgcac acagttaaaa cggtacctaa atttgattgg
      721 ttaaacgggg agaaccttga tggtaagtaa gaaaaaagca ttagaaatga ttgatgttat
      781 agcaaatatg tttccggatg cagaatgtga attaaaacat gataatccgt tcgaattaac
      841 tattgctgta ttattgtcag cgcaatgtac agacgttcta gtaaatagag taacaactga
      901 gctgtttaaa aaatacaaaa cgcctgaaga ttatttggca gtcagtgatg aagaacttat
      961 gaatgatatt cgttctatcg gtctgtatcg taacaaagct aagaatatta agaaactttg
     1021 tcaatctttg attgatcaat ttaatggaga aataccacaa acacataagg aattagaaag
     1081 tttagcaggt gtaggacgta aaactgctaa tgtagtcatg agtgtagcat ttgatgaacc
     1141 ttcgttagct gttgatacgc atgtagaacg tgtttctaaa cgcttaggta ttaatcgttg
     1201 gaaagataat gtgagacagg tagaagaccg tttatgttct gtcataccta gagatagatg
     1261 gaataggagc catcatcaac tcattttctt tggaagatac cactgtttag cccgaaaacc
     1321 taaatgcgat atttgtccac tattagaaga ttgtagagaa ggacaaaaac gttataaagc
     1381 tagtttgaaa gaagcgtgat aatatgacaa atattacaaa agaggtattt gataatttag
     1441 aacaagagat tgatgtattt gccaaaaata agacattagg ttctagtgaa gcaaaacctt
     1501 acttggatga ataccatagt aaaattattg attattttaa gcaggttaat gacatcactg
     1561 gcaatattga ttttgataat ttaaatcaat atcctgttgt gccaatgaat tttaaagaaa
     1621 gatatgatta tatgattgaa cgtaaatatc attttatggg ctatcgacaa atgaagacct
     1681 ttaaaactga attgattaaa atgaatgcta gttatcaaac aaggttaaag aataagcagg
     1741 tatagacaag tttgatcat
//
LOCUS       DAHHOJ010000204         1753 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.209, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000204 DAHHOJ010000000
VERSION     DAHHOJ010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.209"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            58..408
                     /locus_tag="NBH32_002673"
     CDS             58..408
                     /locus_tag="NBH32_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN family protein"
                     /protein_id="HCD1516208.1"
                     /translation="MKIKKYIVAGTLAVLLSTTAVSTLNGNKAEASSYDNYLTQSKYH
                     DKKIAEELKSLLRQSNVYDLAAGSLNRYYKRTIMMNEYRAKAALKKNDFKSMAEAKVG
                     LENIYKEIDEIINR"
     gene            complement(661..846)
                     /locus_tag="NBH32_002674"
     CDS             complement(661..846)
                     /locus_tag="NBH32_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516209.1"
                     /translation="MNKHINKKTTKHLEDYKKVQQRNSEETNRGCLKSVAMLIVLFLF
                     IIFGIVACSTHVNFFFQ"
     gene            complement(1463..1696)
                     /locus_tag="NBH32_002675"
     CDS             complement(1463..1696)
                     /locus_tag="NBH32_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000231333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516210.1"
                     /translation="MVFKDWKVNVVYTGKHEVVTNENNLFVIDEDYDVAIAINYIDNK
                     LKVSHVNYGSEFTIDSSNKVLELMIHNPNIDEN"
BASE COUNT          679 a          227 c          240 g          607 t
ORIGIN      
        1 ctttcctaaa attgactata taatcattaa gtatcaattt ttaaaggaga atttacaatg
       61 aaaattaaaa aatatatagt agcaggaaca ttagcagtac tattatcaac aacagcagta
      121 tcaacgttaa acgggaacaa agcagaagca agtagttatg acaattattt aactcaaagt
      181 aaatatcacg ataaaaaaat tgctgaagag ttgaaatcac tacttagaca atcgaacgta
      241 tatgacttgg ctgcaggtag cttaaatcga tattacaaac gtacgattat gatgaatgaa
      301 tatagagcaa aagcagcact aaagaaaaat gatttcaaat caatggctga agctaaagtt
      361 ggattagaaa acatttacaa agaaattgat gaaattataa atagataata aagaaaacag
      421 gttgagacaa taaatatcat tgtcttcgcc tgttttttaa tttgtatagt taaaaattct
      481 acaatttaat atttatgtat gagcaatgaa tatacaataa taggggagac taatacaaat
      541 ataatagtta gaaatgttat gcacaaaatt gctttgtaca atctaaaaaa tatttataat
      601 gctcccatag ataaaatcaa atgctgtaag aaacgtagtg aatattactc aatcagcata
      661 ttattggaag aaaaagttaa cgtgtgtaga acatgccaca ataccaaata taatgaataa
      721 aaataagacg ataagcatgg cgacactttt taaacaacct ctatttgttt cttccgaatt
      781 tctttgttga acctttttat aatcttcaag atgctttgtg gtctttttat ttatatgttt
      841 attcatgatg tttactcctt aatctaaatg taaattgcat taaaatagtt gaaaacatac
      901 cttgaaataa agatataaag ctaaagttga gaagagtgtt attttaaatt taagcaaaat
      961 taactgtaaa taagttaatt tgatgataac atgtttcgct tatttttaaa attgatagtt
     1021 tttaaactga cgcattaaaa tattaatgaa acactagaac acataaatga ataataaagg
     1081 tgcgcagtat agaattatta atgcaacact aagtataaat attgttaact gtaaactatc
     1141 tttagtttta atataaacaa ttaagataag cagtgttaaa agaaccatat atattaatga
     1201 cgaagatgtc catacaaaat ctgcgtctgt cgtagttaac agcggtacta taattagtcc
     1261 gaagttaatc atgcatgcta tatatacaat gatgttatac acaatgttaa tttttggtca
     1321 ccaccttagc tgttatattt taaatgcttg gaaatcgtgc atttttaagt gtaatgatta
     1381 tctgaatctt tgattaccgt ttgtttttcg tttaaaagac tatatatagt atgcctttat
     1441 atttctaaat gttttttaat atttaattct cgtcaatatt tggattatga atcatcaatt
     1501 caagtacttt gttagatgag tcaattgtaa attcagatcc atagttaaca tgactgacct
     1561 ttaatttatt gtctatataa ttgattgcga ttgcgacatc ataatcttca tctatgacaa
     1621 ataaattatt ttcatttgta acaacttcat gtttcccagt gtaaacaaca ttaactttcc
     1681 aatctttaaa aaccatttgt catcctcctt gaacatttaa actgattcaa tttaagttta
     1741 acttattcat aca
//
LOCUS       DAHHOJ010000205         1716 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.211, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000205 DAHHOJ010000000
VERSION     DAHHOJ010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.211"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(13..1142)
                     /locus_tag="NBH32_002676"
     CDS             complement(join(13..896,896..1142))
                     /locus_tag="NBH32_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558209.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCD1516211.1"
                     /translation="MTRERRSFSSEFKLQMVRLYENGKPRNEIIREYDLTPSALGKWI
                     KQHQNTGTFNHQDNLSDEEKELIKLRKEVQHLKMENDIFKASSADHGTKIEVIQKNAH
                     QYSVSAMCKVLKIPRSTYYDSIKRRDNKNTKDDSNVERAVINIFNSNRKVFGTRRIKN
                     NLNDKGLTVSRRKIARIMKKHNLVSVYTKAKYKNHPKETNEKRIKNHLNRAFNREQPM
                     ETLVSDLTYVKVGGTWHYICLFIDLFNREIVGYSAGKNKDANLVSKAISRINHNLEQI
                     KLFHTDRGKEFDNHLIDEVLETFKIKRSLSTKGCPYDNAVAEATMKAMKTEFVKQMQF
                     ENLEQLETELFDYVNWYNNFRPHSSLQYLTPVAFKNLHMKTV"
     gene            1213..>1716
                     /locus_tag="NBH32_002677"
     CDS             1213..>1716
                     /locus_tag="NBH32_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCD1516212.1"
                     /translation="MQSDWQSSGVPFLRARDIVAYNKGEDIKEPLFISYDKYNEYSSI
                     SDKVENGDLLVTGVGTIGVPFLIKDNTPLYFKDGNIIWLKNRGSLDEYFLFYSFINAN
                     IQSYIKKVAGVGTVGTYTIANAKKTPIFEPIKLEQLKIGGFFNKIDNLIKLQVHKVEI
                     IKQRKQGL"
BASE COUNT          537 a          263 c          250 g          666 t
ORIGIN      
        1 gtcaacattt ttttagacag ttttcatgtg tagattttta aatgccactg gcgtcaaata
       61 ctgtaatgaa gaatgtggtc taaaattgtt gtaccaattt acataatcaa ataattctgt
      121 ttctaactgt tctaagtttt caaattgcat ttgttttaca aattcggttt tcattgcttt
      181 catcgttgct tccgcaactg cgttatcata aggacaacct ttggtactta atgaacgttt
      241 gattttaaat gtttctagga cttcatctat caaatgatta tcaaattctt tgcctctatc
      301 agtgtggaat agtttgattt gttcaagatt atgatttatt ctgctgattg cttttgatac
      361 taaatttgcg tccttatttt tacctgcact gtaaccaaca atttctctat taaatagatc
      421 tataaataaa catatgtaat gccatgttcc tccgactttt acatatgtca aatcacttac
      481 taatgtctcc attggttgtt ctctattaaa agcgcgattc aaatgatttt taattcgttt
      541 ttcattagtt tcttttggat gatttttgta tttagctttc gtataaacag aaactagatt
      601 atgttttttc atgatacgag ctatctttcg ccttgataca gtaagaccct tgtcatttaa
      661 attattttta attcgtcttg taccaaagac ttttctatta gaattaaaaa tatttatgac
      721 agcacgttct acgtttgaat catctttagt gtttttatta tctcttcttt ttatagaatc
      781 ataataagta cttcttggta tttttaggac tttacacatt gctgatactg aatattgatg
      841 tgcattcttt tgaatgactt ctattttcgt cccatgatca gcgctgcttg ctttaaaata
      901 tcgttctcca tttttaaatg ttgaacttct ttacgtaatt taatcagctc tttttcttca
      961 tctgataagt tatcttggtg attgaatgta cccgtgtttt gatgttgctt tatccatttt
     1021 cctaacgccg aaggtgttaa atcatactcg cgtataattt cattcctagg cttaccattt
     1081 tcatataatc taaccatttg taacttaaac tctgaactaa atgatcttct ttctcttgtc
     1141 ataataaaat cgcctacttt cttaaattaa caatatctat tctcatagaa tttgtccaat
     1201 taagtgtaga cgattcaatc agactggcaa tctagtggag tacccttttt aagagcaaga
     1261 gacattgtgg catataataa aggggaagat attaaagaac ccctattcat atcttatgat
     1321 aaatataatg aatatagctc aatatcagat aaagttgaaa atggcgattt attagtaacg
     1381 ggtgtgggca caattggagt accattttta ataaaagata atacgccgct ttactttaaa
     1441 gatggtaata ttatttggtt aaagaataga gggagtttgg atgaatattt tctattttat
     1501 tcttttataa atgcaaatat tcaaagctat ataaaaaaag tggcaggtgt gggtactgtt
     1561 ggcacataca caatagctaa tgcaaaaaaa acaccaattt ttgaaccaat aaaattagaa
     1621 caattaaaaa tagggggatt tttcaataaa atcgataatt taattaagtt acaagttcac
     1681 aaagtagaaa taattaaaca acgtaaacaa gggtta
//
LOCUS       DAHHOJ010000206         1693 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.212, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000206 DAHHOJ010000000
VERSION     DAHHOJ010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.212"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            120..299
                     /locus_tag="NBH32_002678"
     CDS             120..299
                     /locus_tag="NBH32_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516213.1"
                     /translation="MKFKTTKECKSNNIFKRSQEINNRESEKGCLWGISMLILLFLLI
                     LFGITACSSSIHFIN"
     gene            610..870
                     /locus_tag="NBH32_002679"
     CDS             610..870
                     /locus_tag="NBH32_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516214.1"
                     /translation="MCIVIYLIYSLIVYFICIINFFIMAAPFLMTNADYVWTPMTTVT
                     LFILSLIIFLIFIKTKDVVHLTIFILNLLFSILYCLPILFYL"
     gene            complement(923..1273)
                     /gene="scn"
                     /locus_tag="NBH32_002680"
     CDS             complement(923..1273)
                     /gene="scn"
                     /locus_tag="NBH32_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complement inhibitor SCIN-A"
                     /protein_id="HCD1516215.1"
                     /translation="MKIRKSILAGTLAIVLASPLVTNLDKNEAQASTSLPTSNEYQNE
                     KLANELKSLLDELNVNELATGSLNTYYKRTIKISGQKAMYALKSKDFKKMSEAKYQLQ
                     KIYNEIDEALKSKY"
BASE COUNT          586 a          221 c          215 g          671 t
ORIGIN      
        1 agaattccaa ttttataata atattgcttg acatatcaaa ctagatagta ctattttgaa
       61 tatattatta taatcaaaaa ttcattgtaa acttttagac aaaaggaagt aataaaaacg
      121 tgaaatttaa aacaactaaa gaatgtaaaa gtaataatat ctttaaaaga agtcaagaaa
      181 ttaataatag agaaagtgaa aagggttgtt tatggggcat tagcatgttg attctacttt
      241 tcttattgat tctgtttgga ataactgctt gttcatcaag cattcacttt attaattaga
      301 tttttttact tggaggtatc atgtgaagaa ccatacaaat ataattaata tcttattagt
      361 tatagtcaac tcattaactc attttctaac tctaaacacc tcatttttta ataattcagc
      421 atcggatttc tgttttatca taggggctat atttttctta atcggaattt ttgttgcaat
      481 atacggtatg aagcgagcaa catattggtt aaacttattg attttattta ccaatatttt
      541 ttattttcta cacttctgtg tgttactttt gttaaaatat ataggattta aattatttat
      601 ttatgaaggg tgtgtattgt tatttatcta atttatagtc taattgtcta tttcatctgc
      661 attataaatt ttttcataat ggctgcacca ttcttaatga ctaatgctga ttatgtatgg
      721 acgcctatga ctacagttac gttatttatt ttgagtttga ttattttctt gatatttata
      781 aaaacaaaag atgtcgttca tttaacaatt ttcatattaa acttactttt ttcaatactt
      841 tattgtttgc ctatactgtt ttatttatga acacttttat attatctaga aaattaaaac
      901 cacccgtaaa agggtggttt ttttaatatt tactttttag tgcttcgtca atttcgttat
      961 aaatcttttg aagttgatat tttgcttctg acattttctt aaagtctttt gacttaagag
     1021 catacattgc tttttgacct gaaattttta tagttcgctt ataataagtg tttaaacttc
     1081 cagtagctaa ttcattaaca tttagttcat ctaataacga ttttaattca ttagctaact
     1141 tttcgttttg atattcattc gatgttggca agcttgtgct agcttgtgcc tcatttttat
     1201 ctagattagt tactagtggt gatgctaaaa cgattgctaa agttcccgca agtatagatt
     1261 ttctaatttt cattttttat tctccttagt aattagtact aatttatttt aatgactaac
     1321 taaaaaaaag tacattaatt attcattaac tatatagatc ttcttattaa atgaaaatta
     1381 ataaattatc aaattgacat accttattgt atttatagaa aaacaaaaaa aggtaagcac
     1441 cgaaatgctt acctacttcc cataaacaat ataacacata tacattgatt tggaaagcgc
     1501 aaaaataaat ttaatatact agcccgaagg ggagcaatac ataaaaaatg aaaggcgctc
     1561 cttgaaaacg cccaaggtaa tattaacata aaatagctac tattgcatta tctaatttta
     1621 tttaattaaa acaaatatat atagcacaaa aaaactagcc cgaaggctag cttatgaata
     1681 gatgaaaatt tga
//
LOCUS       DAHHOJ010000207         1630 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.213, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000207 DAHHOJ010000000
VERSION     DAHHOJ010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.213"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(67..975)
                     /gene="rocF"
                     /locus_tag="NBH32_002681"
     CDS             complement(67..975)
                     /gene="rocF"
                     /locus_tag="NBH32_002681"
                     /EC_number="3.5.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginase"
                     /protein_id="HCD1516216.1"
                     /translation="MTKTKAIDIIGAPSTFGQRKLGVDLGPTAIRYAGLIPRLKQLDL
                     DVNDKGDIKVSAVDIEKFQSEQNGLRNYDEIIDVTQKLNKEVSASIENNRFPLVLGGD
                     HSIAVGSVSAISKHYNNLGVIWYDAHGDLNIPEESPSGNIHGMPLRILTGEGPKELLE
                     FNINTIKPENIVLIGMRDLDKGERQFIKDKNIKTFTMADIDKLGIQTVIENTLDYLKT
                     HNVDGIHLSLDVDALDPLETPGTGTRVLGGLTYRESHFALELLHQSQSVTSMDLVEVN
                     PLIDSNNHTAEQAVSLVGTFFGETLL"
     gene            complement(1119..1191)
                     /locus_tag="NBH32_002682"
     tRNA            complement(1119..1191)
                     /locus_tag="NBH32_002682"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1156..1158),aa:Lys,seq:ttt)
     gene            complement(1195..1269)
                     /locus_tag="NBH32_002683"
     tRNA            complement(1195..1269)
                     /locus_tag="NBH32_002683"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1235..1237),aa:Gln,seq:ttg)
     gene            complement(1279..1362)
                     /locus_tag="NBH32_002684"
     tRNA            complement(1279..1362)
                     /locus_tag="NBH32_002684"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1326..1328),aa:Tyr,seq:gta)
     gene            complement(1391..1466)
                     /locus_tag="NBH32_002685"
     tRNA            complement(1391..1466)
                     /locus_tag="NBH32_002685"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1431..1433),aa:Val,seq:tac)
     gene            complement(1474..1545)
                     /locus_tag="NBH32_002686"
     tRNA            complement(1474..1545)
                     /locus_tag="NBH32_002686"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1510..1512),aa:Glu,seq:ttc)
     gene            complement(1547..1621)
                     /locus_tag="NBH32_002687"
     tRNA            complement(1547..1621)
                     /locus_tag="NBH32_002687"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1587..1589),aa:Asn,seq:gtt)
BASE COUNT          499 a          341 c          269 g          521 t
ORIGIN      
        1 attttagtga attaaaaaaa gtagtgcaaa aagcaaaata tactttatac actacaaatc
       61 atttatttat aataaagttt caccgaaaaa tgttccaact aatgaaaccg cttgttcagc
      121 agtatgatta ttactgtcaa tcaatggatt tacttcaact aaatccattg atgttactga
      181 ttgagactga tgtaataatt ccagcgcaaa atgactttct ctatatgtaa gtccacctaa
      241 tactcttgta cctgtgcctg gcgtttcaag tggatctaag gcatcaacgt caagagataa
      301 atgtatacca tctacattat gagtttttaa ataatctaat gtattttcaa ttacagtctg
      361 aattcctagt ttatctatgt cagccatagt aaatgtttta atatttttat ctttaataaa
      421 ttgtcgttca cctttatcca aatctctcat accaattagc acaatatttt caggtttaat
      481 ggtattaata ttgaattcta atagttcctt tggtccttca cctgtcaaaa tccgaagtgg
      541 cataccatga atatttccac ttggtgactc ttcaggtata tttaaatcac catgtgcatc
      601 ataccaaata acacctaaat tattataatg tttacttatt gctgatactg aacctaccgc
      661 aatagaatga tctccaccaa gaactagagg aaatctgtta ttttcaattg atgctgaaac
      721 ttctttattt aatttttgag taacatctat aatttcatcg tagtttctta atccattttg
      781 ttcactttga aatttttcaa tgtctacagc tgatacctta atatcccctt tgtcattgac
      841 atcaaggtct aattgcttta gtcttggtat taaacctgca tatctaatcg cagttggtcc
      901 taaatcaaca cctaattttc tttgtccaaa tgttgatggt gcaccaataa tatcaattgc
      961 ttttgtcttt gtcataagcg tccccccttt gttatacata atttcagaat aatgtatacg
     1021 cttactataa tcaagtaata gccataacat aaccccttag cattctaatt ctcaaaaaat
     1081 aaaaaaaagc acttcccaag aatggaaagt gcaagtagtg agccatagag gattcgaacc
     1141 tctgaccctc tgattaaaag tcagatgctc taccaactga gctaatggct ctaatggctg
     1201 ggctagctgg attcgaacca acgagtgacg gagtcaaagt ccgttgcctt accgcttggc
     1261 tatagcccaa tatatagatg gtggaggggg gcagattcga actgccgaac ccgaaggagc
     1321 ggatttacag tccgccgcgt ttagccactt cgctacccct ccagcttatt cagttaatta
     1381 ataatcaaaa tggtggagaa tgacgggttc gaaccgccga ccctctgctt gtaaggcaga
     1441 tgctctccca gctgagctaa ttctccaata taatgactcc tacgggactc gaacccgtgt
     1501 taccgccgtg aaagggcggt gtcttaaccg cttgaccaag gagccatggc tccacaggta
     1561 ggactcgaac ctacgaccga tcggttaaca gccgatagct ctaccactga gctactgtgg
     1621 attaatatta
//
LOCUS       DAHHOJ010000208         1602 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.214, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000208 DAHHOJ010000000
VERSION     DAHHOJ010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1602)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1602)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1602
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.214"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..1112
                     /gene="scpA"
                     /locus_tag="NBH32_002688"
     CDS             <1..1112
                     /gene="scpA"
                     /locus_tag="NBH32_002688"
                     /EC_number="3.4.22.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine protease staphopain A"
                     /protein_id="HCD1516217.1"
                     /translation="TPIANAESNSNIKAKDKKHVQVNVEDKSVPTDVRNLAQKDYLSY
                     VTSLDKIYNKEKASYTLGEPFKIYKFNKKSDGNYYFPVLNTEDNIDYIVTISPKVTKD
                     SSSSSKYTINVSSFLSKALNQYKDQQITILTNSKGYYVVTQNHKAKLVLKTPRLEDKK
                     VKKTESIPTGNNVTQLKQKASVTMPTSQFKSNNYTYNEQYVNKLENFKIRETQGNNGW
                     CAGYTMSALLNATYNTNKYHAEAVMRFLHPNLQGQQFQFTGLTPREMIYFGQTQGRNP
                     QLLNRMTTYNEVDNLTKNNKGIAILGSRVESRNGMHAGHAMVVVGNAKLDNGQEVIII
                     WNPWDNGFMTQDAKNNVIPVSNGDHYRWYSSIYGY"
     gene            1143..1469
                     /locus_tag="NBH32_002689"
     CDS             1143..1469
                     /locus_tag="NBH32_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphostatin A"
                     /protein_id="HCD1516218.1"
                     /translation="MEQIELFSIDKFKCNSEAKYYLNIIEGEWHPQDLNDSPLKFILS
                     TSDDSDYICKYINTEHKQLTLYNKNNSSIVIEIFIPNDNKILLTIMNTEALGTSPRMT
                     FIKHKS"
BASE COUNT          638 a          231 c          244 g          489 t
ORIGIN      
        1 ttacccctat tgcaaacgcc gagagcaatt caaatatcaa agcaaaagat aagaagcatg
       61 tgcaagttaa tgtcgaggac aagagtgttc ctactgacgt acgtaattta gcgcaaaaag
      121 actatctttc ttatgttact tcacttgata aaatctataa taaagagaaa gcctcgtata
      181 cacttggtga accttttaaa atttacaaat tcaacaaaaa gagtgatggt aattattatt
      241 ttcctgtttt gaacactgaa gataatatcg attatatagt tacaatttca ccaaaagtca
      301 ctaaggattc tagttcatct tctaaataca caatcaatgt ttcttcattt ttatctaaag
      361 ccttaaatca atataaagat caacaaatta ctattttgac aaactcaaaa ggttactatg
      421 tggttactca aaatcataaa gcaaaattag tactaaaaac accacgtcta gaagataaaa
      481 aggtaaaaaa gaccgagagt atacctactg gcaataatgt aacgcaacta aagcaaaaag
      541 cttctgtcac tatgccaact agtcaattta aaagtaataa ttatacttac aacgagcaat
      601 atgtaaataa acttgagaat ttcaagataa gagaaactca aggtaacaat ggttggtgtg
      661 caggctatac gatgtctgca ttactaaatg caacttataa tactaacaaa tatcatgcag
      721 aagctgtgat gagattttta cacccaaact tacaaggaca acaattccaa tttactggtt
      781 taacgccaag agagatgatt tattttggac aaactcaggg aagaaatcca caacttctta
      841 atagaatgac tacatataat gaagttgaca atcttactaa aaataataaa ggaattgcta
      901 ttttaggtag tagagtagaa tcaagaaatg gtatgcacgc agggcatgcc atggtagttg
      961 ttgggaatgc taaattagac aatggacaag aggttataat tatatggaat ccatgggata
     1021 atggttttat gactcaagat gctaaaaaca atgttattcc agtatccaat ggcgaccatt
     1081 atcgatggta ttcatctatt tatggttatt aaaatttgta ttttagaaag gtaaataata
     1141 ttatggaaca aattgaatta tttagtattg ataaattcaa atgtaattca gaggctaagt
     1201 attatcttaa tattattgag ggagaatggc atcctcaaga tttaaatgat agtcctttaa
     1261 aatttattct cagtacctca gacgattctg attacatttg caaatatata aatacagaac
     1321 acaaacaact cacattatat aataaaaata atagctcaat tgttattgaa atatttatac
     1381 caaatgataa taaaatacta ctaacaatta tgaacacaga agctttggga acttctccta
     1441 gaatgacttt cattaagcat aagtcataag actatctaga tatgattaag taaactatta
     1501 atcttattaa aggtctctag gaagaaaaaa tttcatgcga tgagattgtt ttagaaaaag
     1561 cgacgatata tataatttaa caatgatttt aaaatattaa ga
//
LOCUS       DAHHOJ010000209         1587 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.215, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000209 DAHHOJ010000000
VERSION     DAHHOJ010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.215"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            30..818
                     /locus_tag="NBH32_002690"
     CDS             30..818
                     /locus_tag="NBH32_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1516219.1"
                     /translation="MNKVTINPQIQLTYQIEGKGDPIILLHGLDGNLAGFEDLQHQLA
                     SSYNVLTYDLRGHGKSSKSESYDLNDHVEDLKILMEKLNIHEAHILGHDLGGVVAKLF
                     TDKYAYRVKSLTTIASKKDDLIHSFTQLLIQYQDDIAGFNKSEAYILLFSKLFRNQEK
                     TMKWYQKQRIYSTKSEDDSAVAIRSLILHKDEPMYLKKRTCVPTLLINGEHDPLIKDK
                     NHFKLEAHFLNVTKKIFEHSGHAPHIEEPEAFMNYYLNFLKSVS"
     gene            complement(1069..1443)
                     /locus_tag="NBH32_002691"
     CDS             complement(1069..1443)
                     /locus_tag="NBH32_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SarA family transcriptional regulator"
                     /protein_id="HCD1516220.1"
                     /translation="MAITKINDCFELLSMVTYADKLKSLIKKEFSISFEEFAVLTYIS
                     ENKEKEYYLKDIINHLNYKQPQVVKAVKILSQEDYFDKKRNEHDERTVLILVNAQQRK
                     KIESLLSRVNKRITEANNEIEL"
BASE COUNT          551 a          213 c          245 g          578 t
ORIGIN      
        1 gtatatagtt tattgaggga ggtgtcacaa tgaataaagt cacaattaat cctcaaatcc
       61 aattaactta tcaaattgaa ggtaaagggg atcctataat attacttcat ggattggatg
      121 gtaatttagc tggatttgaa gatttgcaac atcaactagc atcatcatat aatgtactta
      181 cttacgattt aagaggtcat ggcaagtctt ctaaaagtga atcatacgat ttaaacgatc
      241 acgttgagga tttaaaaatt ctaatggaga agttaaatat tcatgaggca catattctag
      301 gacatgattt aggtggggta gttgctaagt tatttacaga taaatatgct tatcgtgtaa
      361 aatcattaac taccattgca tcgaagaaag atgacttaat acacagcttt actcaattgt
      421 taatacaata tcaagatgat atagcgggtt ttaataagtc tgaagcgtat attcttttat
      481 tttctaaatt gtttagaaat caagagaaga cgatgaaatg gtatcaaaaa caaagaatat
      541 atagcactaa gtctgaggat gatagtgcgg tggcaattcg ttcattaatt ttgcataaag
      601 atgaacctat gtatttaaaa aaacgtacat gtgtacctac tttgttaatt aatggggaac
      661 atgatccttt gattaaagat aaaaatcatt ttaaattgga agcgcatttt ttaaatgtta
      721 cgaaaaaaat cttcgaacat tcaggacatg caccgcatat tgaagaacca gaagcattta
      781 tgaattatta tttaaatttt ttaaaaagcg tatcataata tgtgatatat aaacctaggg
      841 cataaagtcc ttaggcaatg tgaaaaagct gattactatt cattatttga tagaaatcag
      901 ctttttttga aatgtatttg atatatactg ctcgttatgc ggctatcttc cttatattaa
      961 gtgccattag tgcaaaacct cttaacaatt aggtaaaaag agcataaaaa aaggaagttt
     1021 aatagaatgt atcatctatc aaacttcacc aaattgcgct aaacaaaatt atagttcaat
     1081 ttcgttgttt gcttcagtga ttcgtttatt tactcgactc aataatgatt cgattttttt
     1141 acgttgttgt gcattaacaa gaattaatac agttctttca tcatgctcat tacgtttttt
     1201 atcgaagtaa tcttcttgag ataaaatttt aactgcttta acaacttgtg gttgtttgta
     1261 gtttaaatga ttaataatat ctttaagata gtattctttc tctttgtttt cgctgatgta
     1321 tgtcaataca gcgaattctt caaagctaat tgaaaattcc tttttaatta aactttttaa
     1381 tttgtcagca taagtgacca ttgataacaa ctcaaagcaa tcattgattt ttgtaattgc
     1441 catgtttaaa acctccctat ttgatgcatc ttgctcgata catttgcccg ataatatatt
     1501 gatatctaat ctttatttat tatagatatg ttagtcataa ttttgcatta aataagtttt
     1561 attaaatata tttaatgctc tattatt
//
LOCUS       DAHHOJ010000210         1515 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.216, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000210 DAHHOJ010000000
VERSION     DAHHOJ010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1515
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.216"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..1035)
                     /locus_tag="NBH32_002692"
     CDS             complement(<1..1035)
                     /locus_tag="NBH32_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit IIC"
                     /protein_id="HCD1516221.1"
                     /translation="MSNAKNADNKQFFSKILNSIGAGVVIALVPNALLGEVLKIFKSG
                     NEILELTYQLVILIQSFMAFIIGVLAAHQFKFNGAGAAIVGTSAMIGSGAVVYSNNSF
                     MLKGIGDIINTSLVVIIACLIYMVLQNKLGSFELIILPVLVPIVSGGIGLITLPYIRK
                     ITQAIGNVIHSFTDLNPLLMSILISVAFSLLMVTPISLVAIATAISLNGLGSGAANLG
                     IVAACVTFLFGSLRVNSIGVNAVLLIGAAKMMIPVYLKNLIISIPLTINGIITGIIAY
                     VLQVKGTPLSAGFGYTGLVGPINAFNRMSGDPTMNIILLALGYFVIPFVSAYIVHELC
                     KKFIPIYSNDI"
BASE COUNT          595 a          260 c          185 g          475 t
ORIGIN      
        1 aatatcatta gaatatatag gaatgaattt tttacacaat tcatgaacaa tatatgccga
       61 tacaaatggt atgacaaaat aaccaagtgc taataaaata atattcattg ttggatcacc
      121 tgacattcgg ttaaatgcat ttattgggcc aactaatcct gtgtatccaa atccagcaga
      181 caatggtgtt cctttaacct gaagaacgta agcaatgatt ccagtaatta ttccatttat
      241 agtcaatggt atcgaaataa ttaaattttt caagtaaact gggatcatca tttttgcagc
      301 tcctatgagt aaaactgcat ttacaccaat agaattgaca cgcaatgagc caaataagaa
      361 agtaacacag gcagccacta tacctaggtt tgctgctcca cttcctagtc cgtttaaact
      421 aatcgcagtt gcaatcgcta ctaacgatat tggtgttacc attaataatg aaaatgccac
      481 actaataagt atagacatta acaacggatt taagtctgta aaagaatgaa ttacatttcc
      541 aattgcttga gtaatttttc gaatgtaagg taatgtgatt agaccgatac ccccactaac
      601 gataggtact aaaactggta atataattaa ttcaaaagat ccaagtttgt tttgtaatac
      661 catatatata agacatgcaa taataacaac caaactcgta tttataatgt cacctatacc
      721 ttttaacata aaactattat tgctatatac aacagcacct gaaccaatca tagctgatgt
      781 acctactata gcagcacctg cgccattaaa tttaaattga tgagcagcta aaaccccaat
      841 aataaatgcc ataaatgatt gaattagtat cactaactga tacgttaatt ctaaaatttc
      901 attaccactt ttaaatattt ttaatacttc acctaataaa gcattcggaa caagtgcaat
      961 aacaacacca gcaccaatag aatttaaaat cttactgaaa aactgcttat tatcagcatt
     1021 ctttgcgtta ctcataaacg acctccaatt tgaacattaa actcatcata ttatagaaaa
     1081 ttaactttaa caatgattag ttgactaatt taatctaaat ataacaaacc acaaaaggaa
     1141 taaagtaaat ttgaaaatct actcactaat caatgtcatg ttaattttaa aatattacta
     1201 attacatcct gtgactaata atgtaataaa ttgtaaatat taaaatatcc atattggcat
     1261 taagatagat aaattgaatc gacttgctat aaagcacaat aaaatagaac ttttacaaat
     1321 attttattag aaaagtttaa acaaatacat aaccccagat aatatagtca ttatcattaa
     1381 tgtaatacca aaaaaagaag ttaaacaatc tatagttgct taacttccta tattgaactc
     1441 atcagtacaa tttgacatag atcctattaa agcctgtgcc atttgagagc cacttttatt
     1501 tgtattgtat aaaga
//
LOCUS       DAHHOJ010000211         1500 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.217, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000211 DAHHOJ010000000
VERSION     DAHHOJ010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1500)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1500)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1500
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.217"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            170..>1500
                     /locus_tag="NBH32_002693"
     CDS             170..>1500
                     /locus_tag="NBH32_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="The YSIRK form of extended signal peptide directs
                     nascent proteins to the cross-wall site, while signal
                     peptides lacking YSIRK direct proteins instead to the cell
                     pole. A large fraction of YSIRK proteins are surface
                     proteins anchored by sortase-mediated processing of a
                     C-terminal LPXTG motif; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-type signal peptide-containing protein"
                     /protein_id="HCD1516222.1"
                     /translation="MRDKKGPVNKRVDFLSNKLNKYSIRKFTVGTASILIGSLMYLGT
                     QQEAEAAENNIENPTTLKDNVQSKEVKIEEVTNKDTAPQGVEAKSEVISNKDTIEHEP
                     SVKAEDISKKEDTPKEVADVAEVQPKSSVTHNAETPKVRKARSVDEGSFDITRDSKNV
                     VESTPITIQGKEHFEGYGSVDIQSNPEDLKVSEVTRFNNKSIGKNELTGALQLKNKVS
                     FKNDFEFNIRVANNHQSVTTGADGWGFLFSKGDGNEYLQKGGILGPKGMENSAGFKID
                     TGYNFKDPMDKEEKQAGQGFKGYGTFVKTGADGTTAKVGTNIPTRGKADNSFQYADNS
                     IDTTDGKFHGQLLNNLKLAYNEKSGIMRAEYAGKIWEANISDLGLDKSEAYNFLITSS
                     QRQGTSQGVYANGWMRTDLNNSTFKLTPNLVNKTETKTVEIPFETKREFNP"
BASE COUNT          616 a          195 c          290 g          399 t
ORIGIN      
        1 aaactaaaaa taataatttt atttgtagat tgttttaagt gtttcaatat ataaaataat
       61 tttataaaga taagtgttat attccattgt gcaactccta ttgtatttgt aaaattacat
      121 gtaaattcac tgtaagtaaa gtggaaaata tggaaaaagg agtatgcaaa tgagagataa
      181 gaaagggccg gtaaataaaa gagtagattt tctatcaaat aaattgaata aatattcaat
      241 aagaaaattt acagttggaa cagcatctat tttaattggc tcactaatgt atttgggaac
      301 tcaacaagag gcagaagcag ctgaaaacaa tattgagaat ccaactacat taaaagataa
      361 tgtccaatca aaagaagtga agattgaaga agtaacaaac aaagacactg caccacaggg
      421 tgtagaagct aaatctgaag taatttcaaa caaagacaca atcgaacatg aaccatcagt
      481 aaaagctgaa gatatatcaa aaaaggagga tacaccaaaa gaagtagctg atgttgctga
      541 agttcagccg aaatcgtcag tcactcataa cgcagagaca cctaaggtta gaaaagctcg
      601 ttctgttgat gaaggctctt ttgatattac aagagattct aaaaatgtag ttgaatctac
      661 cccaattaca attcaaggta aagaacattt tgaaggttac ggaagtgttg atatacaaag
      721 caatccagag gatctaaagg tatctgaagt cactcgtttc aataataaaa gtattggaaa
      781 aaacgaattg acaggagcat tacaattaaa gaataaagtt tcttttaaaa atgactttga
      841 gtttaacatt agagtagcta ataatcatca atcggtaaca actggcgctg atggatgggg
      901 gttcttattt agtaaaggtg acggtaacga atatttacaa aagggaggta tattaggtcc
      961 caaaggaatg gaaaattcag caggatttaa aattgataca gggtataatt ttaaagatcc
     1021 catggataaa gaagagaaac aggcaggaca aggatttaaa gggtatggta cgtttgtaaa
     1081 aacaggtgct gatggaacta cagcaaaggt tggaacaaac ataccaacaa gaggtaaagc
     1141 ggataattca ttccaatatg cagataattc tattgataca acagatggaa aattccatgg
     1201 tcaattactt aataatttaa aattagcgta caatgaaaaa tcggggataa tgagagccga
     1261 atatgctggt aaaatttggg aagctaatat atcagatttg ggattggata aatctgaagc
     1321 atataacttt ttaattactt ctagtcaaag acagggaaca tctcaaggtg tatatgcaaa
     1381 tggttggatg agaacagatt taaataactc aacatttaaa ctaacgccta atctagttaa
     1441 taaaacagaa acaaaaacag tagagatacc gttcgagaca aaacgtgaat ttaatccgaa
//
LOCUS       DAHHOJ010000212         1483 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.218, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000212 DAHHOJ010000000
VERSION     DAHHOJ010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1483
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.218"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(103..1464)
                     /locus_tag="NBH32_002694"
     CDS             complement(103..1464)
                     /locus_tag="NBH32_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCD1516223.1"
                     /translation="MAEKLQRELSNRHIQLIAIGGAIGTGLFLGAGQTIALTGPSILL
                     TYIIIGFMLFMFMRGLGEIIIQNTEFKSFADVTNTYIGPFAGFVTGWTYWFCWIITGM
                     AEVTAVAKYVSFWFPEIPNWISALFCVLLLMSFNLLSARLFGELEFWFSIIKIATIIG
                     LIVVGFVMILFAYKTQFGHASFTNLYEHGVFPKGASGFFMSFQMALFSFVGIEMIGVT
                     AGETKNPVKTIPKAINSVPIRILIFYVGALAVIMSIIPWQQVDPDNSPFVKLFALIGI
                     PFAAGLINFVVLTAAASSCNSGIFSNSRMLFGLSSQQQAPPNFSKTNKYGVPHVAIFA
                     SSALLLVAALLNYIFPDATKVFTYVTTISTVLFLVVWGLIIIAYINYSRKNPDLHKKA
                     TYKLFGGKYMGYLIFVFFIFVFGLLFINVETRRAIYFIPIWFILLGFMYLRYKRIAAK
                     SNN"
BASE COUNT          539 a          270 c          244 g          430 t
ORIGIN      
        1 gagaaagtaa aagtattgag ccaagttcgc aatttaatat ataagttgca tataaaaacc
       61 cctgttgaac tttaatatat tcaacagggg taaaacttgt tattagttat ttgatttagc
      121 agcgatacgt ttatatctta agtacataaa tcctaatagt ataaaccaaa tgggaataaa
      181 gtatatcgca cgtcttgttt caacattgat aaacaatagg ccaaacacaa aaataaagaa
      241 tacaaaaatg agatagccca tatattttcc gccaaataat ttatacgtag cctttttatg
      301 aaggtctgga tttttacgac tataattgat ataagcgatg ataatcagtc cccaaacaac
      361 taagaataat actgtagaga ttgttgtcac atacgtaaat accttagtag catcagggaa
      421 aatatagttt aataatgctg ccacgagtaa caatgccgat gaagcaaaga ttgctacatg
      481 tggaacgcca tacttattcg tcttagagaa attcggtggt gcttgttgtt gacttgataa
      541 accgaacagc atacgactat tcgagaaaat accactgtta catgatgaag cagcagcagt
      601 tagtactaca aaattaatca agcctgcagc aaatggaatt ccaattaatg cgaacaattt
      661 tacgaatgga ctgttatcag gatcaacttg ttgccaagga ataatcgaca tgataaccgc
      721 tagagcaccg acataaaata ttaaaattct aataggaaca ctattaatag cttttggtat
      781 tgttttaaca ggatttttcg tttcacctgc cgtaacacca atcatttcga ttcccacgaa
      841 tgagaataag gccatttgga atgacataaa gaatccagat gctcctttag ggaatacacc
      901 gtgctcgtat aggtttgtga aactcgcatg tccaaattga gttttatatg caaatagaat
      961 cataacgaaa cctacgacaa ttaaaccaat gattgttgct attttaatga tagagaacca
     1021 aaattctaac tctccaaata gtctcgcact caataagtta aaagacatta ataacaatac
     1081 gcaaaaaagt gcactaatcc agtttggaat ttctgggaac cagaagctta catactttgc
     1141 tacagctgtt acttcagcca tacctgtaat aatccaacag aaccagtatg tccaaccggt
     1201 tacgaatcct gcaaaaggcc caatgtatgt attggttaca tctgcgaaag atttaaattc
     1261 agtattttga atgatgattt cacctaaacc tcgcataaac ataaataaca taaatccaat
     1321 aatgatatat gttagtaata tagaagggcc agttaatgca atcgtttgac cagcgcctaa
     1381 gaataagcct gtaccaattg cgccaccaat agcaattaat tgaatgtggc gattgctcag
     1441 ttccctttgt aatttttcag ccataataca tctcccttaa ata
//
LOCUS       DAHHOJ010000213         1455 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.219, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000213 DAHHOJ010000000
VERSION     DAHHOJ010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1455
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.219"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            257..1216
                     /gene="hyl"
                     /locus_tag="NBH32_002695"
     CDS             257..1216
                     /gene="hyl"
                     /locus_tag="NBH32_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hemolysin"
                     /protein_id="HCD1516224.1"
                     /translation="MKTRIVSSVTTTLLLGSILMNPVANAADSDINIKTGTTDIGSNT
                     IVKTGDLVTYDKENGMHKKVFYSFIDDKNHNKKILVIRTKGTIAGQYRVYSEEGANKS
                     GLAWPSAFKVQLQLPDNEVAQISDYYPRNSIDTKEYMSTLTYGFNGNVTGDDSGKIGG
                     LIGANVSIGHTLKYVQPDFKTILESPTDKKVGWKVIFNNMVNQNWGPYDRDSWNPVYG
                     NQLFMKTRNGSMKAADNFLDPNKASSLLSSGFSPDFATVITMDRKATKQQSNIDVIYE
                     RVRDDYQLHWTSTNWKGTNTKDKWTDRSSERYKIDWEKEEMTN"
BASE COUNT          559 a          218 c          226 g          452 t
ORIGIN      
        1 ccttggttca taaagagttc attcaacttt gactaagcct cgaaattgaa atgcttcctt
       61 tcaaatatta attaaaagtt aaaaacatat tcttaaaata ttcactcatc atcactcaat
      121 catttaacag ttgctacatt aaatatacta atataaacga tgataaatat ttgatatgtc
      181 tcaactgcat tattctaaat tgacatattg attattgttt cctcaaatcg ttttaaaaaa
      241 tagaaggatg atgaaaatga aaacacgtat agtcagctca gtaacaacaa cactattgct
      301 aggttccata ttaatgaatc ctgtcgctaa tgccgcagat tctgatatta atattaaaac
      361 cggtactaca gatataggaa gcaatactat agtaaaaaca ggtgatttag tcacttatga
      421 taaagaaaat ggcatgcaca aaaaagtatt ttatagtttt atcgatgata aaaatcacaa
      481 taaaaaaata ttagtcatca gaacgaaagg taccattgct ggtcagtata gagtttatag
      541 cgaagaaggc gctaataaaa gtggtttagc ctggccttca gcctttaagg tacagttgca
      601 actacctgat aatgaagtag cacaaatttc tgattactat cctagaaatt caattgatac
      661 taaagaatat atgagtacat taacttatgg attcaacggt aatgtgactg gtgatgattc
      721 tggaaaaatt ggcggtttga ttggtgcaaa tgtttcaatt ggtcatacac tcaaatatgt
      781 tcaaccagat ttcaaaacaa ttttagaaag tccaactgat aaaaaagtag gttggaaagt
      841 tatatttaac aatatggtga atcaaaattg gggaccatac gacagagatt cttggaaccc
      901 agtatacggc aatcaacttt tcatgaaaac aagaaacggt tcaatgaaag cagcagataa
      961 cttccttgat cctaacaaag caagttctct attatcttca gggttttcac cagacttcgc
     1021 tacagttatt actatggata ggaaagcaac caaacaacaa tcaaatatag atgtaatata
     1081 cgaacgagtt cgtgatgact atcaattaca ttggacttca acaaattgga aaggtactaa
     1141 tactaaagat aaatggacgg atcgttcttc agaaagatat aaaatcgatt gggaaaaaga
     1201 agaaatgaca aattaatggt catgcacact caaatattca aattttaaca ttagccgata
     1261 acatcagaaa tgatgtgtgt cggctatttt agttgcagta taaaattaaa catcgatgaa
     1321 tagtaatata tatattatgc ttttcaatat atattcatgc ttataaacac gatataagat
     1381 ttaatttttc agtttcaaac atttttaatt catattgtca aaccctttat ttaaaagttt
     1441 tgaaattgtt caaaa
//
LOCUS       DAHHOJ010000214         1453 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.220, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000214 DAHHOJ010000000
VERSION     DAHHOJ010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1453)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1453)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1453
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.220"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(76..930)
                     /locus_tag="NBH32_002696"
     CDS             complement(76..930)
                     /locus_tag="NBH32_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosaminidase"
                     /protein_id="HCD1516225.1"
                     /translation="MNKHKKGSIFGIIGLVVIFAVVSFLFFSMISDQIFFKHVKSDIK
                     IEKLNVTLNDAAKKQINNYTSQQVSNKKNDAWRDASATEIKSAMDSGTFIDNEKQKYQ
                     FLDLSKYQGIDKNRIKRMLVDRPTLLKHTDDFLKAAKDKHVNEVYLISHALLETGAVK
                     SELANGVEIDGKKYYNFYGVGALDKDPIKTGAEYAKKHGWDTPEKAISGGADFIHKHF
                     LSSTDQNTLYSMRWNPKNPGEHQYATDIKWAESNATIIADFYKNMKTEGKYFKYFVYK
                     DDSKHLNK"
     gene            complement(1206..1430)
                     /locus_tag="NBH32_002697"
     CDS             complement(1206..1430)
                     /locus_tag="NBH32_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516226.1"
                     /translation="MDYAHLNLEHFFARNDDLDVIRDRADFVMINNFTNEMMYRDGQI
                     EGTIDLNQYYYKNRSNAASFIMMDYKKETK"
BASE COUNT          453 a          253 c          171 g          576 t
ORIGIN      
        1 acgctttcta tttattgcga tagaaagcgt ctttttatac ttgagcaagt catatcataa
       61 cttaactcta ataaattact tattcaaatg tttactgtca tctttataca caaagtattt
      121 gaagtatttt ccttcagtct tcatgttctt ataaaagtca gcgataattg ttgcattact
      181 ttctgcccac ttaatatcag tagcatattg atgttctcct ggattttttg gattccatct
      241 catactatac aatgtatttt gatctgtgct tgataagaag tgcttatgaa tgaaatcagc
      301 accgcctgaa atagcttttt caggtgtatc ccaaccatgc tttttagcat attctgcacc
      361 tgttttaatt gggtctttat caagggctcc tactccatag aaattgtagt attttttgcc
      421 atcaatttcg actccattag ctaattcact tttaactgcg ccagtttcta ataatgcatg
      481 tgaaattaaa taaacttcgt taacgtgctt atctttagca gcttttaaga aatcatccgt
      541 atgtttcaat aacgttggtc tatctactaa catacgttta attctatttt tatcaatccc
      601 ttgatacttt gataaatcta aaaattgata tttttgcttt tcattatcga taaaagtacc
      661 gctatccatt gcacttttaa tttcagttgc agatgcatct ctccatgcat cattcttttt
      721 atttgatacc tgttgactcg tataattatt tatttgtttc tttgctgcat cgtttaatgt
      781 aacatttaac ttttcaatct taatgtcgga tttaacatgt ttgaaaaata tctgatcgga
      841 tatcattgag aaaaataaaa atgagacaac agcaaatatg acaacaagtc ctattattcc
      901 aaaaatagaa cctttcttgt gtttattcat atccacacct cttaggtcat tgttgttata
      961 cattcagttt gactacgata ataaagttta acattattaa aaccgtttaa caattttatc
     1021 tatgcattgt tacatattca taaataaaca gtaatgtaaa tgaaacgttt taaacgattt
     1081 atcaacatta ctttacgttg atatacgaat cataaatgtt ttatcttttt gtataacctt
     1141 tgaaaacctt tatatcaata atcttattat tcacgtatta aaaaacaaaa aattctcttg
     1201 ttcgtttact tagtttcttt tttataatcc atcataataa aacttgctgc atttgatcta
     1261 tttttataat agtactgatt taaatcaatc gtaccttcaa tttgaccatc acgatacatc
     1321 atttcattag tgaaattatt tatcatcacg aaatcagcgc gatctcttat aacatctaaa
     1381 tcgtcgtttc gtgcaaaaaa atgttctaaa tttaaatgtg cgtaatccat tgttacctcc
     1441 cgattacaaa ttt
//
LOCUS       DAHHOJ010000215         1414 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.221, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000215 DAHHOJ010000000
VERSION     DAHHOJ010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.221"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            133..483
                     /locus_tag="NBH32_002698"
     CDS             133..483
                     /locus_tag="NBH32_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YppE family protein"
                     /protein_id="HCD1516227.1"
                     /translation="MNDVVESLIYEVNNMQQNFENVKSQQQDHDFYQTVKPYTEHIDS
                     MLNEIKLHREFIIEVPYMNSRKFELLIANIEQLSVECHFKRTSRKLFIEKLKSVQYDL
                     QNILDGVTKEGTHG"
     gene            476..1039
                     /locus_tag="NBH32_002699"
     CDS             476..1039
                     /locus_tag="NBH32_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HCD1516228.1"
                     /translation="MVKTVYVTGYKSFELNIFKDDAPEVHYLKQFIKHKIEQLLDEGL
                     EWVLIQGQMGIELWTAEVVIELQRTYDSLKFAVITPFQGHTEKWNEHNQSKYANIIKH
                     ADYVDSIFHTPYQGPFQFKQADQFMLEHSDQTLLIYDEEQEASPKFFKQMLVDFMDKT
                     NYTCDIVTFDELTAFINDLQWSEDQSF"
     gene            1053..1397
                     /gene="gpsB"
                     /locus_tag="NBH32_002700"
     CDS             1053..1397
                     /gene="gpsB"
                     /locus_tag="NBH32_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HCD1516229.1"
                     /translation="MSDVSLKLSAKDIYEKDFEKTMARGYRREEVDAFLDDIIADYQK
                     MADMNNEVVKLSEENHKLKKELEELRLRVATSRPQDNKSFSSNNTTTNTSSNNVDILK
                     RISNLEKAVFGK"
BASE COUNT          567 a          176 c          241 g          430 t
ORIGIN      
        1 taggaagttt ttagatttac ttgatttaac agataacaca tttaaaaaat agttaaatca
       61 aattatatag agtgttaaaa catgacgaat acagtatatt gtgataaatt aaaaaatgta
      121 ggaggcgtcc tcatgaatga tgtagtcgaa tcactaattt atgaagttaa caacatgcaa
      181 caaaattttg aaaatgtgaa atcacaacaa caagatcatg atttttacca aactgtaaag
      241 ccatatactg aacatattga tagcatgctc aatgagatca aattacatcg tgaatttatt
      301 atagaagtac cttatatgaa ttcaaggaaa tttgagctac tgattgctaa cattgaacaa
      361 ctttctgtcg aatgtcattt taagcgaaca agtcgaaagt tatttattga aaagcttaaa
      421 agtgttcaat atgatttaca aaatatatta gatggcgtaa caaaagaggg tactcatggt
      481 taaaacagtt tatgtaacag gttacaaatc attcgaatta aacattttta aagatgacgc
      541 acctgaagta cattatttaa aacaatttat aaaacataaa attgaacaac tgttggatga
      601 aggattagaa tgggtgttaa tacaagggca aatgggtata gaattatgga ctgcagaagt
      661 tgttattgaa ttacaacgaa catatgattc gttaaagttt gctgtaatta caccgttcca
      721 aggacataca gaaaagtgga atgaacataa tcaaagtaag tatgccaaca taattaagca
      781 tgcagattat gttgatagta tttttcatac gccgtatcaa ggtccttttc aatttaaaca
      841 agcagatcaa tttatgcttg aacattcgga ccaaacattg ctcatttatg atgaggaaca
      901 agaggcgagt cctaagttct tcaagcagat gttagttgat tttatggata aaacaaacta
      961 tacttgtgat attgtgacgt tcgatgaatt aacagcattc atcaatgact tacagtggtc
     1021 tgaagatcaa agtttctaat gaggtggaaa aaatgtcaga tgtttcattg aaattatcag
     1081 caaaagatat ttatgaaaaa gattttgaaa aaacgatggc tcgtggctat agaagagaag
     1141 aagtagatgc atttttagat gacattattg ctgattatca aaaaatggcc gatatgaata
     1201 atgaagttgt aaaattatca gaagagaatc ataaacttaa aaaagaatta gaagaattaa
     1261 gactacgtgt agcaacatct agacctcagg acaataaaag tttttcttcg aataatacaa
     1321 caacaaatac atcttcaaat aatgtagata ttttaaaacg tatttcaaac ttagaaaaag
     1381 ctgtatttgg taaataatta ctaaatataa aagt
//
LOCUS       DAHHOJ010000216         1414 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.222, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000216 DAHHOJ010000000
VERSION     DAHHOJ010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.222"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(262..1353)
                     /locus_tag="NBH32_002701"
     CDS             complement(262..1353)
                     /locus_tag="NBH32_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase"
                     /protein_id="HCD1516230.1"
                     /translation="MSNKLESYRSEIVSLNHQILDLLSKRGELAQKIGEEKLKQGTRI
                     YDPQREKEMLNDLIDSNKGPFNDNTIKQLFKEIFKASTDLQKSENEKHLYVSRKLKPE
                     DTIVTFDNGGIIGDGNKSFVFGPCSVESFEQVEAVAKNLHAKGEKFIRGGAFKPRTSP
                     YDFQGLGVEGLKILKQIKDKYDLNVVSEIVNPNDFEVADEYLDVFQIGARNMQNFELL
                     KEAGRTKKPILLKRGLSATIEEFVYAAEYIASQGNQNIILCERGIRTYEKATRNTLDI
                     SAVPILKQGTHLPVMVDVTHSTGRKDIMLPTAKAALAVGADGVMAEVHPDPSVALSDA
                     GQQMDLDEFQAFYDELKPLSDLYNAKKLK"
BASE COUNT          447 a          252 c          197 g          518 t
ORIGIN      
        1 ttatatttac agataattca tagttataat tttaattact gcaatcagtc ataatcttta
       61 ataattgtga aattgaacaa gtgttgaagt attataacaa tcgttaagtg cagtacacct
      121 ttatttctat acaaaaaata atagccactc taaaagtaat atactcttag aatggctaca
      181 agtttaaaat tgtcgattca aagataatat tctacttcaa catgacatat tagttaagta
      241 gtctatagtt tccttggaat attattttaa ctttttagcg ttatataaat cagacaaagg
      301 ctttaattca tcataaaatg cttggaattc atctaaatcc atttgttgac ccgcatcact
      361 aagtgcaaca gatggatctg gatgcacctc agccataacg ccatcagcac ctactgctaa
      421 tgctgctttc gcagttggta acatgatatc tttacgacct gtactatgcg taacatctac
      481 catgactggt aagtgcgtac cttgttttaa aattggtact gctgaaatat ctaaagtgtt
      541 acgtgtagcc ttttcataag ttcggattcc acgttcacat aaaataatgt tttgattacc
      601 ttgtgaagca atgtattcag ctgcataaac aaactcttcg attgtagcag ataaaccacg
      661 ttttaataga ataggctttt tcgtacggcc agcttctttt aataactcga agttttgcat
      721 attacgtgca ccaatttgga atacgtctaa atactcatca gccacttcaa aatcatttgg
      781 atttacgatt tcactgacaa catttaaatc atatttatct ttaatttgtt taagtatttt
      841 aagaccttca acacctaaac cttggaaatc atatggtgat gtacgtggtt taaatgcacc
      901 gccacgaata aatttttcac ctttagcatg taagttttta gcaacagctt caacttgttc
      961 aaatgattca actgaacatg gcccaaatac aaatgattta ttgccgtctc caataatgcc
     1021 cccattatca aatgttacaa tcgtatcttc aggtttcaac ttacgtgata catataaatg
     1081 tttttcattt tcagattttt gtaaatctgt agaggctttg aaaatttctt taaataattg
     1141 cttaatagta ttatcgttga atggtccttt gttactatcg attaagtcgt taagcatttc
     1201 tttttcacgt tgtggatcat agatacgtgt accttgtttt aatttttctt ccccaatttt
     1261 ttgtgctagt tcaccacgtt tagataataa gtctaaaatt tgatgattca gtgagacaat
     1321 ctcacttctg tatgattcta atttattact catccttact cacctctacg aattatttca
     1381 agtatattcc actttaaagc aataaaatat aatg
//
LOCUS       DAHHOJ010000217         1377 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.224, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000217 DAHHOJ010000000
VERSION     DAHHOJ010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.224"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            163..1317
                     /locus_tag="NBH32_002702"
     CDS             163..1317
                     /locus_tag="NBH32_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCD1516231.1"
                     /translation="MKLSLNSNSKYLRAPSIRQFSNRMKGLDDCVNLTIGQPDFPMPD
                     VVKRAYIDAINNNQTSYSHNKGLLETREAISQYFKNRYHFYYDPEEIIVTNGASEAID
                     TTLRSIIEPGDEIIIPGPIYAGYIPLIEVLGGKPIYIDTTATQFKITPDALESHISPK
                     TKAVLLNYPTNPTGVVLKRNEVLNIVNVLKKYPIFIISDEIYAENTFSGKHVSFAEFE
                     DIRDQLILIGGLSKSHSATGIRIGFLLGPQYLIEKLTFMHAYNCICANVPAQIACITA
                     LNEGLEAPKYMNEAYVERRNYLVSELTKLGFEITAQPEGAFYIFPSIKHITDDDFEFC
                     VDLLESTHLAIVPGSSFTEFGKGFVRISYAYEMDVLKEGMKRLAKYLNTK"
BASE COUNT          484 a          209 c          216 g          468 t
ORIGIN      
        1 ctttattcat tttgaaaagt agttactaca gtgtatttag acatttttcg tttttcaaat
       61 aaagcttaag taatttaccc ttttttgctt taaaagtatg ttatgatatt attaatttct
      121 aaatttttag acaatatttg atttatattt gaggtgtcac aaatgaaact ttctttaaat
      181 tctaattcta aatatttaag agccccaagt attcgccaat tttcaaaccg catgaaaggt
      241 cttgatgatt gtgttaattt aacaattggg caacctgact ttcctatgcc agacgtagtg
      301 aaacgtgcat atatcgatgc cataaacaat aatcaaactt catactctca caataaaggg
      361 ttattagaaa ctcgcgaagc aattagtcaa tacttcaaaa atcgttatca tttttactat
      421 gatcctgaag aaattattgt gacaaatgga gctagtgaag caatagacac aacgttacgt
      481 tctatcatag agcctggaga tgaaattata ataccgggac caatttatgc aggctacata
      541 ccactcatcg aagtactagg tggtaaacca atttatattg atacaacagc aacacaattc
      601 aaaattacac ctgatgcgtt agaaagtcat atttctccaa agacaaaagc tgtcttgtta
      661 aattatccga ctaatccaac tggcgtagtt ttaaaaagaa atgaagtttt aaatatcgta
      721 aatgtattaa aaaaatatcc gatatttatt attagcgatg agatttatgc tgaaaataca
      781 tttagtggta aacatgtatc tttcgctgaa tttgaagaca ttcgtgatca attaatatta
      841 attggtggtt taagtaaatc acactcagca actggaataa gaattggttt tctattagga
      901 cctcaatatc tcattgaaaa attaacattt atgcatgcgt ataattgtat ttgtgccaat
      961 gttccagctc aaatagcatg tataacagca cttaacgaag gattagaagc tcctaaatat
     1021 atgaacgaag cttatgtaga acgaagaaat tatttagtat ctgaattaac taaattaggt
     1081 tttgagatta ccgctcaacc tgaaggcgcg ttttatattt tcccaagtat taaacatatc
     1141 accgatgacg atttcgaatt ttgtgtcgat ttacttgaat caacacattt agcaatcgtt
     1201 ccaggctcat cctttacaga attcggtaaa ggatttgtaa ggatttccta tgcttatgaa
     1261 atggatgttt taaaagaagg tatgaagaga cttgctaaat atttaaatac taaataaaaa
     1321 taatatgttg aactggacgt cgtgaactaa attcatagta cgttcagttt tttattt
//
LOCUS       DAHHOJ010000218         1315 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.225, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000218 DAHHOJ010000000
VERSION     DAHHOJ010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.225"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            166..>1315
                     /locus_tag="NBH32_002703"
     CDS             166..>1315
                     /locus_tag="NBH32_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCD1516232.1"
                     /translation="MAKSCLHILTNNEYATTRCQDGIVLFWPIDGEIELQKFRKSKII
                     EDDIYIINHLDVFSIKNNKKTIMLYLSSDWFAELGFTFFNYHYTAKLIKSSYNLKCLL
                     LKLTYRYLDNQPLNDADIRKLQDIIKIIAKEASMDKKIAQNQYRYAYYGDLRDELEYI
                     YQNVNQRLTLKSVADKLFVSKSNLSSQFHLLMGMGFKKYIDTLKIGKSIEILLTTDST
                     ISNISEHLGFSSSSTYSKMFKSYMDITPNEYRNLSKFNKCIMLNPEPLVGGMAQEVKE
                     VILNYIDHYKNHLTDVIHIDEDKFEIPKLFQTVVQINTYTEMKLVFLEGIFKTLLNKN
                     SQVVFFIMPSILKSKNTMSEEEKFTIIKTIIESDLKIAFNINDIETTYY"
BASE COUNT          510 a          155 c          202 g          448 t
ORIGIN      
        1 gtaatgttta aatgattgat atataagttt aaaaaataac cattatatag ttttatttat
       61 ttttgatttg gtattcgtat tgtgttaaat ttacgtttaa ttaaataagt taaatattat
      121 agggaatgat aaaagtggta tttaaatata gaaagaggta aagttatggc aaaatcatgc
      181 ttgcatatac ttactaataa tgaatatgcg acaacgcgtt gccaagatgg catagtctta
      241 ttttggccaa ttgacgggga aatcgaacta caaaaatttc gtaaaagtaa aataattgaa
      301 gatgatatat atatcattaa tcatctggat gtatttagta ttaagaataa taaaaaaacg
      361 atcatgttgt atttgagtag cgattggttt gcggaattag gctttacttt ctttaattat
      421 cactatacag caaagttaat taaatcatcc tataatttga aatgcttatt attaaaattg
      481 acatatcgat accttgataa tcagcctctt aatgacgctg atattagaaa attacaggat
      541 attattaaaa tcattgcaaa agaagcaagt atggataaaa agattgcgca aaatcaatat
      601 cgttatgctt attatggtga tttgcgagat gagctcgaat atatttatca aaatgtaaat
      661 caacgattga cattaaaaag tgtcgctgat aaattatttg tttcaaagtc aaatctgtct
      721 tcgcaattcc acttacttat gggcatgggt tttaaaaaat atattgatac tttgaaaatt
      781 ggtaaatcca tagagattct acttactaca gatagtacta ttagcaatat aagtgaacat
      841 ttaggattta gcagcagctc aacgtactct aaaatgttta aaagttatat ggatataaca
      901 ccgaatgaat atcgtaattt atcaaaattt aataaatgta taatgcttaa tccagagcca
      961 cttgtaggcg gtatggccca agaagtaaaa gaagtcatat tgaattatat tgatcattat
     1021 aaaaatcacc taactgatgt tatacatatt gacgaagaca aatttgaaat acctaagtta
     1081 tttcaaacgg ttgttcaaat taatacttat acagaaatga aacttgtttt cttagaagga
     1141 atttttaaaa ccttattgaa caagaacagc caagttgtct ttttcatcat gccatcgatt
     1201 ttaaaaagta aaaataccat gtccgaagaa gaaaagttta caatcattaa aaccataatt
     1261 gaaagtgatt taaagatagc atttaatata aatgatattg aaacgactta ttatg
//
LOCUS       DAHHOJ010000219         1258 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.226, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000219 DAHHOJ010000000
VERSION     DAHHOJ010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.226"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            76..441
                     /locus_tag="NBH32_002704"
     CDS             76..441
                     /locus_tag="NBH32_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000214067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="truncated hemoglobin"
                     /protein_id="HCD1516233.1"
                     /translation="MTTTPYDIIGKEALYDMIDYFYTLVEKDERLNHLFPGDFAETSR
                     KQKQFLTQFLGGPNIYTEEHGHPMLRKRHMDFTITEFERDAWLENMQTAINRAAFPQG
                     VGDYLFERLRLTANHMVNS"
     gene            464..>1258
                     /gene="yjbH"
                     /locus_tag="NBH32_002705"
     CDS             464..>1258
                     /gene="yjbH"
                     /locus_tag="NBH32_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease adaptor protein YjbH"
                     /protein_id="HCD1516234.1"
                     /translation="MAGELRIMENKSREDINLSPVSKIEIYSFFDPFSSDCFKLSAIL
                     SKLRIEYNQYIRIRHILNPSLKVLTKCQAQSTSNFDNIALAYKAAELQGRVRAERFIH
                     LMQNEIIPKRDIITESMICDCIQNAGIDLEVFKDDLQKSKLTESLKIDLHIAREMEIE
                     QAPSLVFFSEDVHEEGLKVEGLYPYHIYTYIINELMGKPIEKNLPPKLETYIQQQQLV
                     TMEELLTIYEWPEKLLNKELKKLAIQQKIEKLKYPDGDFWKSKMPKI"
BASE COUNT          487 a          190 c          202 g          379 t
ORIGIN      
        1 tgtttaaaat catgttatat tagttatata ttaacttaag ttcgtaacag taaatttttg
       61 aaaatgggtg attaaatgac aacaacacca tatgacatca ttggtaaaga agcgttatac
      121 gatatgattg attattttta cacccttgta gaaaaagatg aacgacttaa tcacctgttt
      181 ccaggagatt ttgcagaaac aagtcgtaaa caaaaacaat ttttaacaca gtttttgggc
      241 ggtccaaaca tttataccga agaacacgga catcctatgc taagaaaaag acatatggat
      301 tttacaatca ctgaatttga aagagatgca tggttagaaa atatgcagac agcaattaat
      361 cgcgctgcgt ttccacaagg tgttggagat tatttatttg agcgcttaag attaactgct
      421 aatcatatgg tgaattccta aaattaattg taggtgaata aacatggctg gagaattacg
      481 aataatggaa aataagagtc gtgaagatat taatctatca cctgtaagta aaatcgaaat
      541 ttattcattt ttcgatccat ttagctccga ttgcttcaaa ttatcagcaa tcttatccaa
      601 attaagaatt gaatataatc aatatatacg tatcagacat atattaaatc cttcgttaaa
      661 agtattaacg aaatgccaag ctcaaagtac atccaacttt gataacatcg ccctagctta
      721 taaagcagct gagttacaag gtcgtgtacg tgccgaacga tttatacatt taatgcaaaa
      781 tgaaatcata cctaaaagag atattattac agaatcaatg atttgtgact gtattcaaaa
      841 tgcaggtatt gatttagaag tatttaaaga cgacttacaa aaaagtaaac taaccgaaag
      901 cttgaaaatt gatttgcata ttgcaagaga aatggaaatc gaacaagccc cttctctcgt
      961 tttctttagt gaagatgttc atgaagaagg tttaaaagtc gaaggattat acccatatca
     1021 catctatact tatataatta atgaattgat gggtaaacct atcgaaaaga atcttcctcc
     1081 taaattagaa acttatatac agcaacaaca acttgtaacg atggaagaat tacttactat
     1141 ttatgaatgg ccagaaaaac ttttaaacaa agagttaaag aagttagcca ttcaacaaaa
     1201 aattgaaaaa cttaaatatc cagatggcga tttctggaaa tctaaaatgc ctaaaatc
//
LOCUS       DAHHOJ010000220         1255 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.227, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000220 DAHHOJ010000000
VERSION     DAHHOJ010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.227"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..446
                     /gene="seu"
                     /locus_tag="NBH32_002706"
     CDS             <1..446
                     /gene="seu"
                     /locus_tag="NBH32_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="staphylococcal enterotoxin type C1/U"
                     /protein_id="HCD1516235.1"
                     /translation="SDNMELNDGRLIEKTCMYGGVTEHDGNQIDKNNSTDNSHNILIK
                     VYENERNSLSFDIPTNKKNITAQEIDYKVRNYLLKHKNLYEFNSSPYETGYIKFIEGS
                     GHSFWYDLMPESGKKFYPTKYLLIYNDNKTVESKSINVEVHLTKK"
     gene            485..1240
                     /gene="sen"
                     /locus_tag="NBH32_002707"
     CDS             485..1240
                     /gene="sen"
                     /locus_tag="NBH32_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001236366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type N"
                     /protein_id="HCD1516236.1"
                     /translation="MRLFYIAAIIITLLCLINNNYVNAEVDKKDLKKKSDLDSSKLFN
                     LTSYYTDITWQLDESNKISTDQLLNNTIILKNIDISVLKTSSLKVEFNSSDLANQFKG
                     KNIDIYGLYFGNKCVGLTEEKTSCLYGGVTIHDGNQLDEEKVIGVNVFKDGVQQEGFV
                     IKTKKAKVTVQELDTKVRFKLENLYKIYNKDTGNIQKGCIFFHSHNHQDQSFYYDLYN
                     VKGSVGAEFFQFYSDNRTVSSSNYHIDVFLYKD"
BASE COUNT          522 a          126 c          194 g          413 t
ORIGIN      
        1 catcggataa tatggaatta aatgatggta gattaattga aaaaacgtgt atgtatggcg
       61 gtgtgaccga gcatgatgga aatcaaatag ataaaaataa ttcaactgat aactctcata
      121 atatcttaat taaagtttat gaaaacgaga gaaattcatt atcttttgat atacctacta
      181 ataagaaaaa cataacagca caagaaatag attataaagt tagaaactat ttacttaagc
      241 ataaaaattt atatgaattt aacagttcgc cttatgagac tggctatata aagtttatcg
      301 aaggaagtgg tcattctttt tggtatgatt tgatgcctga atctggtaaa aaattttatc
      361 cgactaaata tttactaatt tataatgata ataaaacagt tgagagtaaa tctattaatg
      421 tagaagttca tttaaccaaa aaataattga gggagagaat attataaaaa atattaaaaa
      481 gcttatgaga ttgttctaca tagctgcaat tataataact ttattatgtc ttattaataa
      541 taattatgtt aatgctgaag tagacaaaaa agatttaaag aaaaaatctg atctagatag
      601 tagtaagtta tttaatttaa caagctatta tactgatata acgtggcaat tagacgagtc
      661 aaataaaatt agtacagatc aactactgaa taatactata atattaaaaa atattgatat
      721 atccgtactt aaaacttcta gtttgaaagt tgagtttaac tcatcagatt tagcaaatca
      781 atttaaagga aaaaatatag atatttatgg actgtatttt ggaaataaat gtgtaggctt
      841 aactgaagaa aaaacatcat gcttatacgg aggagttacg atacatgatg gaaatcaatt
      901 agatgaagag aaagttatag gcgttaatgt atttaaagat ggtgtccaac aagaaggttt
      961 tgttataaaa accaaaaagg ctaaagtaac agtacaagaa ttagacacta aagttcgatt
     1021 taaattagaa aatttatata aaatatacaa taaagatacc ggtaacatac aaaaaggatg
     1081 cattttcttt cattctcata atcatcaaga tcaatcattt tattatgatt tatataacgt
     1141 aaaaggttca gtgggagcag agttttttca attttatagt gataatagaa cagttagctc
     1201 atctaattat catattgatg tatttttata taaagattaa gtgattgatg attat
//
LOCUS       DAHHOJ010000221         1216 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.229, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000221 DAHHOJ010000000
VERSION     DAHHOJ010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1216)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1216)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1216
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.229"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            191..>1216
                     /locus_tag="NBH32_002708"
     CDS             191..>1216
                     /locus_tag="NBH32_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCD1516237.1"
                     /translation="MNRDNKWTMITALFITVISVLLAFHLKQHYDQTTNENHANKDKI
                     NIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKN
                     PLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVVIGG
                     DSAGAQLASQFTAIQTNDRLRKAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRI
                     QLFMKSYTGEEDWEKSFKNISQMSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQ
                     ELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQI
                     ENPSNELP"
BASE COUNT          459 a          185 c          195 g          377 t
ORIGIN      
        1 tttcaaacgt aaaaatgcgg ataaaaaaca tattgcttaa tatcatatga tggatatccg
       61 aaactttaca attgaatcac tttgttttaa ccttaaaagc aattcgtctc tactcttatt
      121 gggcgaattg ctttttatat ttattcagtc tattaatatg agcgtctaac aaatagagag
      181 gtacgatgta atgaatagag ataataaatg gacgatgata actgcgcttt ttataactgt
      241 aatcagtgta ttgttagcat ttcatctgaa acaacattat gaccaaacta caaatgagaa
      301 ccatgctaat aaagacaaaa ttaatattaa aaataaaaat gtgcgcattt atcaaaatct
      361 tacatacaat agagttttcc ctaacagtaa attagatatt attacacctg ttgatatgtc
      421 ttctaatgcc aaactgccag ttattttttg gatgcacggt ggtggttata ttgcgggtga
      481 taagcagtat aaaaacccat tattagcgaa aattgctgaa caagggtaca ttgttgtgaa
      541 tgtaaattat gcattggcgc cacaatataa atatcccaca ccattaattc aaatgaatca
      601 agcaactcaa ttcattaaag aaaataaaat gaatttacct attgatttta atcaagtagt
      661 tattggcggt gattctgctg gcgcacaact ggctagtcaa tttacggcaa tacagacgaa
      721 tgatcgctta agaaaagcca tgaaatttga tcagtcattc aaaccatcgc aaattaaagg
      781 tgctatacta tttggtggtt tttataatat gcaaacagtt agagaaactg agtttccaag
      841 aatacagtta tttatgaaaa gttatactgg cgaagaagat tgggaaaaga gttttaaaaa
      901 catttcacaa atgtcgacag taaaacaatc gacaaaaaat tatccaccaa catttttatc
      961 tgttggagat agcgatccat tcgaaagtca aaatatagaa ttcagtaaga aattacaaga
     1021 attgaatgta ccagtagata ctttgtttta tgatggtacg catcatttac atcatcagta
     1081 tcaatttcac cttaataaac ctgaatcgat agataatatc aaaaaagtgt tactgttctt
     1141 aagtcgtaat acatcttcta gtggtattca aactgaagag aaaccacaaa tagaaaatcc
     1201 gagtaatgaa ttaccg
//
LOCUS       DAHHOJ010000222         1203 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.230, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000222 DAHHOJ010000000
VERSION     DAHHOJ010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.230"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..658
                     /locus_tag="NBH32_002709"
     CDS             <1..658
                     /locus_tag="NBH32_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001561168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1828 domain-containing protein"
                     /protein_id="HCD1516238.1"
                     /translation="FIRIYADTLPNNEICLSDDGLTINELEMLGIDINTKTRTKLIQN
                     ILNQFNLKLVDKEITADVKNESFAQSKHNLIQGILKIYDLTLTTKSNVTNIFYEEVFE
                     FLYDQEIRGLAQVSVSGESGLKYSIDYIVSETKSQPEKLVNFTNNLNFNKITNEAYIY
                     RDIKPNRPSRNKLEPIMLIIVNDVDHPINERAQTVAEHENLEILKWSNKSKIIETLTS
                     "
BASE COUNT          501 a          171 c          148 g          383 t
ORIGIN      
        1 ttttattaga atatatgctg atacactacc taataatgaa atttgtctat cagatgacgg
       61 attgacgatt aatgaattag aaatgttggg tattgatata aacacaaaaa cgagaactaa
      121 acttatacaa aatattttaa atcaatttaa tttaaaatta gttgataaag aaattacagc
      181 ggatgttaaa aatgagagtt ttgctcagtc taaacataat ttaattcaag gcatattgaa
      241 aatttatgac cttactttaa ctactaaatc taacgttaca aacatattct atgaagaagt
      301 atttgaattc ttatatgacc aagaaataag aggtttagca caagtttctg tttctggaga
      361 atcaggttta aaatattcta ttgattatat tgtttctgaa acaaaatcac aaccagaaaa
      421 actagttaac tttacaaaca acttaaattt caacaaaata actaatgaag cttatattta
      481 ccgtgatatc aaaccaaaca gaccatccag aaacaaatta gaacctatca tgttgataat
      541 tgtaaatgat gtagatcatc cgataaatga aagagcacaa actgtagcag aacatgaaaa
      601 ccttgagata ttaaaatggt ctaataaatc aaaaatcatt gaaactttaa cctcataaat
      661 tagcagtagt cccttattta tattctttaa tttccaaacc atatcgtgca aatagaaaag
      721 cagataactt ttttatttgc acgttttatt ttagaacaaa aaagaacaag gtcaaagagt
      781 cagaaaaact ttttcttgat acgtatttga cacttgctct tttaagaaaa ctaacaattc
      841 attgtatttc cgttaaatta acggagaatt cggattacct agatagattt acctctagag
      901 tgttaaaaaa ttgtacattc ttaaacaatc ataaatgtac gttattaaat tatcatttcc
      961 actggagaat catgtcgact aaaagattat aaaagtaaaa atacatttaa aagcacgtca
     1021 ttttcaaacc gccataaaac agcattttta aaccgctatt gacataaaac atatttagaa
     1081 taaatcattt agaaaaatta aataatttaa ccggcaataa actaactatt tacatgaatc
     1141 tataaaacac ttagaacaaa agttcgaaaa acgcacttta tgattgtata gtgatatcaa
     1201 ata
//
LOCUS       DAHHOJ010000223         1179 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.232, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000223 DAHHOJ010000000
VERSION     DAHHOJ010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1179
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.232"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..978)
                     /locus_tag="NBH32_002710"
     CDS             complement(<1..978)
                     /locus_tag="NBH32_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516239.1"
                     /translation="MMMNKEATKIGFAYVGIVVGAGFSTGQEVMQFFTKYGLWAYLGV
                     IISGFILAFIGRQVAKIGTAFEATNHESTLQYVFGEKFSKVFDYILIFFLFGIAVTMI
                     AGAGATFEESYNIPTWLGALIMTLAIYVTLLLDFNKIVRALGVVTPFLIVLVVLIAGV
                     YLFKGHVSLAEVNQVVPEASIWKGIWFGTIYGGLAFSVGFSTIVAIGGDTEKRTVSGA
                     GAMYGGIIYTVLLALINFALQSEYPTIKNASIPTLTLANNIHPLIATVLSVIMLAVMY
                     NTILGLMYSFAARFTEPYSKNYHIFIIIMMVAGYLLSFVGFAELINKLYT"
BASE COUNT          421 a          223 c          163 g          372 t
ORIGIN      
        1 tgtatataac ttattaatta attcagcaaa tcctacgaaa cttaataaat aacctgctac
       61 catcattata ataataaaga tatgataatt tttactgtat ggttctgtaa aacgtgctgc
      121 aaatgaatac attagtccta gaatagtatt atacataacc gcaagcataa taacagataa
      181 cacggttgct attaacggat gaatgttatt ggctaatgtt aatgttggaa ttgatgcatt
      241 tttaattgta ggatattcac tttgtaaagc aaagttgatc aatgctagta atacagtata
      301 gataatacca ccatacatcg cgcctgcacc tgacactgta cgtttttcag tatccccacc
      361 gattgctacg atggtactaa aacctacaga aaaagctaat ccaccatata ttgtaccaaa
      421 ccagattccc ttccagatac ttgcttcagg cactacttgg ttaacttctg ctaatgaaac
      481 atgaccttta aataaataaa cgccagcgat taatacaact aaaacaatta aaaatggtgt
      541 aacaacacca agggcacgta ctatcttatt aaagtctaat agcaatgtta cataaattgc
      601 taatgtcata attaatgcac ctagccatgt aggtatgtta taactttctt caaatgttgc
      661 gcctgcacca gctatcatgg ttacagctat accaaataag aagaagatta aaatataatc
      721 aaagacttta ctaaactttt caccgaatac gtattgtaat gttgattcat ggtttgtcgc
      781 ttcaaaggca gtaccaattt ttgctacttg gcgcccaata aaagctaaaa taaaaccaga
      841 tataatcaca cccaaataag cccacaagcc atatttagtg aaaaattgca taacttcttg
      901 tcctgttgaa aatcctgcgc ccactacaat gccgacgtag gcaaatccaa tttttgttgc
      961 ttctttattc atcatcatgt gaataataga cctccttaat tttgcacagt gtgcattata
     1021 acgtactgaa aattaattgc aagtcacata ggttattttt cactttttca aaattgctca
     1081 atccattcac attatcacgt tcaatgacat ttaaaaaaat tataaattta tgcttttata
     1141 atgcagtcat acgaaccttt gtataggaaa aatgattaa
//
LOCUS       DAHHOJ010000224         1175 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.234, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000224 DAHHOJ010000000
VERSION     DAHHOJ010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1175)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1175)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1175
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.234"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..155
                     /locus_tag="NBH32_002711"
     CDS             <1..155
                     /locus_tag="NBH32_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HCD1516240.1"
                     /translation="CEHLITQRKLNYQKEHYLETLSSSFGNREMDDINQIAIDNLNAI
                     GELYDE"
     gene            148..909
                     /gene="istB"
                     /locus_tag="NBH32_002712"
     CDS             148..909
                     /gene="istB"
                     /locus_tag="NBH32_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HCD1516241.1"
                     /translation="MNNSTNYKRLKANLEYLKMNQMINHLDDVIDFSIKNNLSFIDTL
                     IKLSDYEIEIKEKNLIESMVKVAAFPFKKEISDFDFNFQPSINQQEIWDFTDLRFIEK
                     YQNIVFLGSSGVGKTHLATGIGMAAAKNRVSTYFIKCHHLIENLRKAKLENRLENRLK
                     HYSKYKLLIIDEIGYLPIGSEDAKLFFQLIDLRYEKKSTIFTTNINFNLWNEIFDDPK
                     IANAILDRILHHSSVVKIKGKSYRLKDHSPKKEIT"
BASE COUNT          422 a          171 c          187 g          395 t
ORIGIN      
        1 tttgtgaaca tctcatcaca caacgtaaat taaattatca aaaagaacat tatttagaaa
       61 cactgtcatc ttcttttgga aatagagaaa tggatgatat taatcagatt gcgatagata
      121 atttgaatgc gattggagag ttgtatgatg aataattcga cgaattataa gcgcttaaaa
      181 gcgaatttag aatatttgaa aatgaatcaa atgattaatc atcttgatga tgttatcgat
      241 tttagtatta aaaataacct gtcatttata gatacactaa ttaaattaag tgactacgaa
      301 atcgaaatta aagagaaaaa tttgattgaa tcgatggtaa aggtggcggc atttccgttt
      361 aaaaaagaaa tatcagactt tgactttaat tttcaaccga gcatcaatca acaagaaatt
      421 tgggatttta cagatttgcg ttttatagaa aaatatcaaa acattgtctt tttaggctca
      481 agtggcgttg gcaaaacaca tttagctaca ggcattggta tggctgcagc taaaaataga
      541 gtgagtactt actttattaa atgtcatcat ttgatagaga atttaagaaa agcaaaactt
      601 gagaacagat tagaaaatag attgaagcac tatagcaagt ataagttatt aatcatagat
      661 gagattgggt acttacctat tggaagtgaa gatgccaaac tattctttca attaatagat
      721 ttaagatatg agaagaaaag tacaatattt accacaaata tcaatttcaa cttatggaat
      781 gaaatatttg atgatccaaa aatcgcaaat gcgattttgg ataggatatt gcatcattca
      841 agtgtagtta aaatcaaagg caaatcgtat agattaaaag atcattcacc taagaaggaa
      901 attacttagc aaacaatttt gtacattctt atacaatcat ttatgtacat ttttatattg
      961 acatttacat cttgacttat gcttctttcc tctccttcgg ctctcgctta ctcatttagc
     1021 tctactaaac tcgttgcact cttttctcgt ttcgtcagat tcaaacgttt tcacttcgcc
     1081 aagccatttt tctttgtgtt tgctttttat tttgacgttt tagacataaa aaaaagagac
     1141 ctcacggtct caatgcggct catcgcatcc atttt
//
LOCUS       DAHHOJ010000225         1108 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.235, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000225 DAHHOJ010000000
VERSION     DAHHOJ010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1108
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.235"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(99..>1108)
                     /gene="cls"
                     /locus_tag="NBH32_002713"
     CDS             complement(99..>1108)
                     /gene="cls"
                     /locus_tag="NBH32_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCD1516242.1"
                     /translation="EYYTFALDGLGQRILKALEEKLKQGLEVKILYDDVGSKKVKMAN
                     FDHFKSLGGEVEAFFASKLPLLNFRMNNRNHRKIIVIDGQLGYVGGFNIGDEYLGLGK
                     LGYWRDTHLRIQGDAVDALQLRFILDWNSQAHRPQFEYDEKYFPKKNKSFGNAPIQIA
                     ASGPASDWHQIEYGYTKMIMSAKKSVYLQSPYFIPDNSYINAIKIAAKSGVDVHLMIP
                     CKPDHPLVYWATFSNASDLLSSGVKIYTYENGFIHSKMCLIDDEIVSVGTANMDFRSF
                     ELNFEVNAFVYDENLAKDLRAAYEHDITKSKQLTEESYANRPLSVKFKESLAKLVSPI
                     L"
BASE COUNT          370 a          213 c          146 g          379 t
ORIGIN      
        1 aatgacattt gtcactttta cggtttacat aatacaatta tagtcattaa ttgcagaaat
       61 aaaaagagcc tctataatcg agactcctta caaataaatt ataaaattgg cgaaactaat
      121 ttagctaacg attctttaaa cttaacagac aatggtctat tagcataaga ttcttcagta
      181 agttgttttg attttgtaat atcatgttca taagccgctc ttaaatcttt agcaagattt
      241 tcatcatata caaaggcatt tacttcaaaa tttaattcaa aacttctaaa gtccatattt
      301 gctgtgccga ctgatacgat ttcatcatca attaagcaca ttttagaatg tataaatcca
      361 ttttcatacg tataaatttt aacaccactt gataataagt cagaggcatt tgaaaatgtc
      421 gcccaataaa ctaaaggatg atctggctta catggaatca tcagatgaac atctacccct
      481 gatttagctg caattttaat ggcatttata tatgagttat ccggaatgaa ataaggcgat
      541 tgcaaatata ccgacttctt agcgctcata atcatttttg tataaccata ttcaatttga
      601 tgccagtcac tggcaggacc acttgcagca atctggattg gcgcattacc gaatgattta
      661 ttctttttag gaaaatattt ttcatcatat tcaaattgag gtcgatgtgc ttgcgaattc
      721 cagtctaaaa taaatcgcaa ctgcagtgca tcaaccgcat cgccttgtat acgtaaatgc
      781 gtatctctcc aataccctaa ttttcctaaa cctagatatt catcaccaat gttaaatcct
      841 ccgacataac ctagttgacc atcgattacg atgatttttc tatgatttct attattcatt
      901 ctgaaattca ataacggtaa tttagaagca aaaaatgctt caacttctcc acctaacgat
      961 ttaaaatgat caaaattagc catcttaact ttctttgaac caacgtcatc atataaaatt
     1021 ttcacttcta gaccttgttt caatttctct tctaacgctt ttaaaattct ttggcctaag
     1081 ccatctagag cgaatgtata atactcta
//
LOCUS       DAHHOJ010000226         1100 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.236, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000226 DAHHOJ010000000
VERSION     DAHHOJ010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.236"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            57..809
                     /gene="sarS"
                     /locus_tag="NBH32_002714"
     CDS             57..809
                     /gene="sarS"
                     /locus_tag="NBH32_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator SarS"
                     /protein_id="HCD1516243.1"
                     /translation="MKYNNHDKIRDFIIIEAYMFRFKKKVKPEVDMTIKEFILLTYLF
                     HQQENTLPFKKIVSDLCYKQSDLVQHIKVLVKHSYISKVRSKIDERNTYISISEEQRE
                     KIAERVTLFDQIIKQFNLADQSESQMIPKDSKEFLNLMMYTMYFKNIIKKHLTLSFVE
                     FTILAIITSQNKNIVLLKDLIETIHHKYPQTVRALNNLKKQGYLIKERSTEDERKILI
                     HMDDAQQDHAEQLLAQVNQLLADKDHLHLVFE"
BASE COUNT          425 a          163 c          133 g          379 t
ORIGIN      
        1 tttcttatta tattgtgtta tattaaaata aagtgcatat acaaggagat aaaacaatga
       61 aatataataa ccatgacaaa attagagatt ttataatcat tgaagcatat atgtttcgtt
      121 ttaagaaaaa agtcaagcct gaagtcgata tgactataaa agaatttata ttactgactt
      181 atttatttca tcagcaagaa aacacacttc catttaagaa gattgtttca gatttatgtt
      241 ataaacaatc ggatttagta cagcatataa aagtacttgt gaaacattca tatattagta
      301 aagttcgaag taaaattgat gagcgtaata cttacatttc aatatctgaa gaacaacgag
      361 agaaaattgc agaacgtgtt acattgtttg atcaaatcat taaacaattt aatcttgcag
      421 atcaaagtga atcacagatg ataccaaaag atagtaaaga atttctaaac ttgatgatgt
      481 atacaatgta tttcaagaat attatcaaaa agcatctaac attaagtttt gtagaattta
      541 caattctagc tattatcact tctcaaaata aaaacatcgt tcttcttaaa gatttaattg
      601 aaacaatcca ccataaatac cctcaaactg ttagagctct caataattta aaaaagcaag
      661 gctatctaat aaaagaacgc tcaactgaag atgaaagaaa aattttaatt catatggatg
      721 acgcgcagca agaccatgct gaacaattat tagctcaagt gaatcaatta ttagcagata
      781 aagatcattt acatcttgtt tttgaataat atctctatta cgcaagtgtg ctgtattcta
      841 aagtacactt gtgttttcta ttttttaata aaacctcagc acattatgaa caactttcta
      901 ttttctatat ctcttaaaac catttccgaa attaaacctc agcacattca aagccccact
      961 ttattcttaa aaatattttt taacttatac gtattaaacc gctttcatta taaaaaatat
     1021 ctctatattt tatctgtttt tattaatcga aatagcgtga ttttgcggtt ttaagccttt
     1081 tacttcctga ataaatcttt
//
LOCUS       DAHHOJ010000227         1057 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.238, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000227 DAHHOJ010000000
VERSION     DAHHOJ010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1057)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1057)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1057
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.238"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(61..1047)
                     /locus_tag="NBH32_002715"
     CDS             complement(61..1047)
                     /locus_tag="NBH32_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylinositol-specific phospholipase C"
                     /protein_id="HCD1516244.1"
                     /translation="MKKCIKTLFLSIILVVMSGLYHSAHASDSLSKSPENWMSKLDDG
                     KHLTEINIPGSHDSGSFTLTDPVKSVWAKTQDKDYLTQMKSGVRFFDIRGRASADNMI
                     SVHHGMVYLHHELGKFLDDAKYYLSAYPNETIVMSMKKDYDSDSKVTKTFEEIFREYY
                     YNNPQYQNLFYTGSNANPTLKETKGKIVLFNRMGGTYIKSGYGADTSGIQWADNATFE
                     TKINNGSLNLKVQDEYKDYYDKKVEAVKNLLAKAKTDSNKDNVYVNFLSVASGGSAFN
                     STYNYASHINPEIAKTIKANGKARTGWLIVDYAGYTWSGYDDIVSEIIDSNK"
BASE COUNT          321 a          215 c          131 g          390 t
ORIGIN      
        1 tagtgaaaat aattagaatc taactatttt catactcgtc ttaatatcat tattgaatcc
       61 ttatttatta ctatctataa tttcgcttac gatatcatca tatccagacc acgtatatcc
      121 tgcatagtca acaatcagcc aacccgttct agctttccca tttgctttaa tcgtttttgc
      181 aatttcagga tttatatgtg atgcatagtt ataagtacta ttaaatgcgc tgcctccaga
      241 cgctacactc aagaaattca catatacatt gtctttgtta ctatccgttt tagctttagc
      301 caataaattt ttaacagctt caactttttt atcatagtaa tctttatact catcttgtac
      361 ttttaaattt aagctaccat tattaatttt cgtttcaaat gtcgcattgt ctgcccattg
      421 aatgccagac gtgtcagcac cataaccact ctttatgtat gtaccaccca ttctattgaa
      481 taggacaatt ttaccttttg tctcttttaa agtaggattc gcgttacttc ctgtgtaaaa
      541 aagattctga tattgtgggt tattataata atattctcta aaaatctctt caaatgtctt
      601 cgtaacttta gaatcgctat cgtagtcctt tttcatagac atcacaattg tttcgtttgg
      661 ataagcactc aagtaatatt tagcatcatc taaaaatttc cctagttcat gatgcagata
      721 aaccatgccg tgatgaactg aaatcatatt atcagcactt gctctgcctc taatatcaaa
      781 aaacctgact cctgacttca tttgggtaag gtaatcttta tcttgagtct ttgcccaaac
      841 tgattttact ggatcagtta aagtgaatga gccactatca tgtgaacccg gtatattaat
      901 ctcagttaaa tgttttccat catcaagttt actcatccaa ttttccggac ttttactcaa
      961 cgaatctgac gcatgtgctg aatgatataa accactcatc actactaaaa tgatacttaa
     1021 aaacaaagtc ttaatacact ttttcataat atcaact
//
LOCUS       DAHHOJ010000228         1055 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.239, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000228 DAHHOJ010000000
VERSION     DAHHOJ010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1055)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1055
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.239"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            69..401
                     /locus_tag="NBH32_002716"
     CDS             69..401
                     /locus_tag="NBH32_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000212550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516245.1"
                     /translation="MATLKDVKTGKKEILPSTAKDKNGKKVTLIYFEKDDKLGFYVQK
                     TQKERGVGKCVSGIAGGAVTGGTTLGLAGAGVGTVTIPVIGTVSGGVVGAVGGAVGGG
                     LTGGATFC"
BASE COUNT          350 a          130 c          212 g          363 t
ORIGIN      
        1 gtagagaact ctattatgct ttttgagtat atgtggggtt ctttctttat atataaaagg
       61 agttcggagt ggcaacacta aaagatgtaa agactggtaa aaaggaaatt ttgccatcaa
      121 ctgcaaaaga taaaaatggt aaaaaagtta cgttaattta ttttgaaaaa gacgataaat
      181 taggttttta tgtgcagaaa acacaaaaag aacgtggagt agggaaatgc gtttctggta
      241 tagcgggtgg cgcagtgaca ggaggcacta ctttaggtct tgcaggtgca ggagtaggaa
      301 cagttactat tccagtaatt gggacagtta gtggaggcgt agttggagct gttggtggtg
      361 ctgtcggcgg tggtctaacc ggtggagcca cattctgcta atagtgtagg agtgatacca
      421 tgaaaaaatt ttttaatata tgggtatgtt cgttcttatt ttttagtaat aatatcatgg
      481 gtgataatta gtataaaatt tggtaatatc agcacgacgc aaatattaat ttcaaatctg
      541 tttatattta tcgcttatat actatattta gttaagttca ttcgtttcaa aaaaagatga
      601 aaatacgttt tttgcccaaa acctaattgt gatagcatat gaaaagtgag tagtgtcctt
      661 tttttaatct aatttactta tgaagcgaaa ataacgtgca agtaacgtgc aagtaaaaaa
      721 acgacctaaa tccctttaaa aacaaggatt aggtcgtatt tatttggtaa tgaatttgtc
      781 tagcgaaaat aacgtgcaag taacgtgcaa ataaaaaagc atgcgtaata tggtatatat
      841 tttatatgtc taggtaaagt ttacgaacct acagatttga aatattcttc agcaactccg
      901 tcttttataa ttcttgaaat aaccgaggtt tttattttta cgttgttttg aattgtctaa
      961 aatagtcata cataatctgt ttaaaccctt acttaagctt gtaaaatgcg gaaataagtt
     1021 ttcctttttt agttattttt tcttggtata tttta
//
LOCUS       DAHHOJ010000229         1032 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.241, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000229 DAHHOJ010000000
VERSION     DAHHOJ010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1032
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.241"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..>1032)
                     /locus_tag="NBH32_002717"
     rRNA            complement(<1..>1032)
                     /locus_tag="NBH32_002717"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          226 a          308 c          227 g          271 t
ORIGIN      
        1 gcacgtgtgt agcccaaatc ataaggggca tgatgatttg acgtcatccc caccttcctc
       61 cggtttgtca ccggcagtca acttagagtg cccaacttaa tgatggcaac taagcttaag
      121 ggttgcgctc gttgcgggac ttaacccaac atctcacgac acgagctgac gacaaccatg
      181 caccacctgt cactttgtcc cccgaagggg aaggctctat ctctagagtt gtcaaaggat
      241 gtcaagattt ggtaaggttc ttcgcgttgc ttcgaattaa accacatgct ccaccgcttg
      301 tgcgggtccc cgtcaattcc tttgagtttc aaccttgcgg tcgtactccc caggcggagt
      361 gcttaatgcg ttagctgcag cactaagggg cggaaacccc ctaacactta gcactcatcg
      421 tttacggcgt ggactaccag ggtatctaat cctgtttgat ccccacgctt tcgcacatca
      481 gcgtcagtta cagaccagaa agtcgccttc gccactggtg ttcctccata tctctgcgca
      541 tttcaccgct acacatggaa ttccactttc ctcttctgca ctcaagtttt ccagtttcca
      601 atgaccctcc acggttgagc cgtgggcttt cacatcagac ttaaaaaacc gcctacgcgc
      661 gctttacgcc caataattcc ggataacgct tgccacctac gtattaccgc ggctgctggc
      721 acgtagttag ccgtggcttt ctgattaggt accgtcaaga tgtgcacagt tacttacaca
      781 tatgttcttc cctaataaca gagttttacg atccgaagac cttcatcact cacgcggcgt
      841 tgctccgtca ggctttcgcc cattgcggaa gattccctac tgctgcctcc cgtaggagtc
      901 tggaccgtgt ctcagttcca gtgtggccga tcaccctctc aggtcggcta tgcatcgttg
      961 ccttggtaag ccgttacctt accaactagc taatgcagcg cggatccatc tataagtgac
     1021 agcaagaccg tc
//
LOCUS       DAHHOJ010000230         1007 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.242, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000230 DAHHOJ010000000
VERSION     DAHHOJ010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1007
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.242"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            37..561
                     /locus_tag="NBH32_002718"
     CDS             37..561
                     /locus_tag="NBH32_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HCD1516246.1"
                     /translation="MNHDKSPIILIGFMGTGKSTIGKYVADEQNLSFIDIDSYIEEKY
                     KLTIPEIFSKHGEQYFRNLEFTCLQECINTADIIATGGGIIESEEAFNFLKNQKNIIW
                     LDCNIDIIYSRINDDPHRPNANNKTIMQLNDLYCSRILRYNEIAFKKFDSHLLSISEI
                     YYELLNLIKASDQY"
     regulatory      548..645
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00504"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="glycine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="glycine"
                     /db_xref="RFAM:RF00504"
     gene            720..>1007
                     /locus_tag="NBH32_002719"
     CDS             720..>1007
                     /locus_tag="NBH32_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCD1516247.1"
                     /translation="MSSDLKQTPLYQNYVDRGAKIVEFGGWAMPVQFSSIKEEHNAVR
                     YEIGLFDVSHMGEIEVTGKDASQFVQYLLSNDTDNLTTSKALYTALCNEEGG"
BASE COUNT          366 a          117 c          194 g          330 t
ORIGIN      
        1 ataagattgt tttgttattg aaagaaggga aaacgcatga atcatgataa atcaccaata
       61 attttaattg gtttcatggg taccggtaaa tctacgattg gtaaatacgt tgcagatgag
      121 caaaatttat catttattga tatagattca tatatcgaag aaaagtataa gttaacaata
      181 ccagaaatat ttagtaaaca tggtgaacaa tatttcagga atttagagtt cacatgtttg
      241 caagaatgta ttaacactgc agatataatt gctactggtg gtggtattat tgaaagtgaa
      301 gaggcattta attttttgaa aaatcaaaaa aacattattt ggttagattg taatattgat
      361 attatatata gtcgaatcaa tgatgaccca catcgaccta atgcaaataa taagacaatc
      421 atgcagttaa atgacttgta ttgctcgcgg attttaagat ataatgaaat cgcattcaag
      481 aaatttgata gtcatttgct atcaatttca gaaatatatt atgaattgct aaatttaata
      541 aaagcgagtg atcagtatta gagagaatag agcgttaaga ctctatcgcc gaaggtgcaa
      601 gtaatttatt acgaaactct caggcaaaag gataatactg taacgcgttc ctgaattggt
      661 gatttataaa cagggtagcg attgctatcc tgtttttata attttaaggg ggtatttcaa
      721 tgtcaagtga tttaaaacaa acacctttat atcaaaatta tgttgataga ggtgcaaaaa
      781 ttgtggaatt cggaggatgg gcgatgcctg ttcaattttc tagtattaaa gaggaacata
      841 atgctgttcg atacgaaata ggcctgtttg atgttagtca tatgggtgaa attgaagtaa
      901 caggtaaaga tgctagtcag tttgtgcaat atttattatc aaatgatact gataatttaa
      961 ctacttcaaa agcattatat actgctttat gtaatgaaga aggcggt
//
LOCUS       DAHHOJ010000231          997 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.243, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000231 DAHHOJ010000000
VERSION     DAHHOJ010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.243"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..268
                     /gene="sem"
                     /locus_tag="NBH32_002720"
     CDS             <1..268
                     /gene="sem"
                     /locus_tag="NBH32_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HCD1516248.1"
                     /translation="RYLQEEYNIYGFNDTNKGRKYGNKSKFSSGFNAGKILFHLNDGS
                     SFSYDLFDTGTGQAESFLKIYNDNKTVETEKFHLDVEISYKDES"
     gene            303..>997
                     /gene="sei"
                     /locus_tag="NBH32_002721"
     CDS             303..>997
                     /gene="sei"
                     /locus_tag="NBH32_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_031927819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type I"
                     /protein_id="HCD1516249.1"
                     /translation="MKKFKYSFILVFILLFNIKDHTYAQGDIGVGNLRNFYTKHDYID
                     LKGVTDKNLPIANQLEFSTGTNDLISESNNWDEISKFKGKKLDIFGIDYNGPCKSKYM
                     YGGATLSGQYLNSARKIPINLWVNGKHKTISTDKIATNKKLVTAQEIDVKLRRYLQEE
                     YNIYGHNNTGKGKEYGYKSKFYSGFNNGKVLFHLNNEKSFSYDLFYTGDGLPVSFLKI
                     YEDNKIIESEKFHL"
BASE COUNT          409 a          110 c          150 g          328 t
ORIGIN      
        1 aagatatcta caagaagaat ataatattta tggctttaat gatacaaata aaggaagaaa
       61 atatggtaat aagtcaaaat ttagttctgg atttaatgca ggaaaaatat tatttcattt
      121 gaatgatggt tcatcatttt cttatgactt atttgatact ggaacaggac aagctgaaag
      181 tttcttaaaa atatataatg acaacaaaac tgtcgaaact gaaaaattcc atttagatgt
      241 agaaatatct tataaggacg aaagttgaag tatttcaatc ataacttagt aaaggaaatg
      301 ccatgaaaaa atttaaatat agttttatat tagtttttat attacttttt aacattaaag
      361 atcatacgta tgctcaaggt gatattggtg taggtaactt aagaaatttc tatacaaaac
      421 atgattatat agatttaaaa ggcgtcacag ataaaaacct acctattgca aatcaactcg
      481 aattttcaac aggtaccaat gatttgatct cagaatctaa taattgggac gaaataagta
      541 aatttaaagg aaagaaactg gatatttttg gcattgatta taatggtcct tgtaaatcta
      601 aatacatgta tggaggggcc actttatcag gacaatactt aaattctgct agaaaaatcc
      661 ctattaatct ttgggttaat ggcaaacata aaacaatttc tactgacaaa atagcaacta
      721 ataaaaaact agtaacagct caagaaattg atgttaaatt aagaagatat cttcaagaag
      781 aatacaatat ctatggtcat aataacactg gtaaaggcaa agaatatgga tataaatcta
      841 aattttattc aggttttaat aatgggaaag ttttatttca tttaaataat gaaaaatcat
      901 tttcatatga tttgttttat acaggagatg gactgcctgt aagttttttg aaaatttatg
      961 aagataataa aataatagaa tctgaaaaat ttcatct
//
LOCUS       DAHHOJ010000232          993 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.244, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000232 DAHHOJ010000000
VERSION     DAHHOJ010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.244"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..294
                     /locus_tag="NBH32_002722"
     CDS             <1..294
                     /locus_tag="NBH32_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCD1516250.1"
                     /translation="IEQFGLSNSLKRKVHTLSGGEQQRVALIRMMLKDPIVMLADEPT
                     GALDPKTGQMIIQSLFDLVDENKVLILATHDMAIANQCDEIIDLEQYRKVASM"
     gene            complement(382..672)
                     /locus_tag="NBH32_002723"
     CDS             complement(382..672)
                     /locus_tag="NBH32_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516251.1"
                     /translation="MFCAYHFKMKIGGVILTRTYNIIGILSCLISFIIMALPMIWYTA
                     SALWFFPGAIMILLLSLVIVFCYIKTKNQLHLLLLVLNIIILLFFSLPLLLS"
     gene            813..950
                     /locus_tag="NBH32_002724"
     CDS             813..950
                     /locus_tag="NBH32_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001788572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="poly(glycerol-phosphate)
                     alpha-glucosyltransferase"
                     /protein_id="HCD1516252.1"
                     /translation="MADKILKLVNNDVLAEEFGSKARENIIEKYSTESILEKWLNLFN
                     S"
BASE COUNT          411 a          118 c          169 g          295 t
ORIGIN      
        1 atagaacagt ttggtctgtc aaacagttta aaaagaaaag ttcatacgct aagtggaggt
       61 gaacaacaac gtgtcgcttt aattagaatg atgttaaaag atccgattgt tatgttagct
      121 gatgaaccaa cgggtgcgtt agatcctaaa acaggacaga tgattattca atcattattt
      181 gatttggtcg atgaaaataa agtgctgatt ttagcaacac atgatatggc tattgcaaat
      241 caatgtgacg aaataataga tttagaacag tatagaaaag tagcatctat gtgatatcca
      301 aaagataaaa ttaaaagcca atcaatggga aatgttgact tcaacactcc gtttgattgg
      361 cttttataat gtgttactaa gttatgaaag taacaacggc aaactgaaaa aaagcaatat
      421 aataatattt aatacgagta gtaataaatg taattgattt ttcgttttaa tatagcaaaa
      481 aacaatgact aaagataaca acaatatcat gattgcacct gggaagaacc ataatgctga
      541 agctgtgtac caaatcattg gtaacgccat aataataaaa gatataagac aagaaaggat
      601 accaataata ttataagttc tagtcaaaat tacacctcca attttcattt taaaatgata
      661 tgcgcaaaac aatcatttta aatacatcat acaataaaat taaattgtgc ggtagaagaa
      721 tttgaaatta aattgaattg gcttgattaa aaggtgattt tatagaagat aataaaaatg
      781 gttatttaat agaaaaccat aatataaacg acatggctga taaaatatta aagcttgtta
      841 ataatgatgt tttagcagag gagtttggtt cgaaagcgag agaaaacatt atagaaaaat
      901 attcaacgga atcaatatta gaaaaatggt taaatctttt caatagctaa atgaaaaaat
      961 cctaggtatt catgagtacc taggattttt acg
//
LOCUS       DAHHOJ010000233          993 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.245, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000233 DAHHOJ010000000
VERSION     DAHHOJ010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 993)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..993
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.245"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>993
                     /locus_tag="NBH32_002725"
     rRNA            <1..>993
                     /locus_tag="NBH32_002725"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          294 a          193 c          280 g          226 t
ORIGIN      
        1 tcgattccct tagtagcggc gagcgaaacg ggaagagccc aaaccaacaa gcttgcttgt
       61 tggggttgta ggacactcta tacggagtta caaaggacga cattagacga atcatctgga
      121 aagatgaatc aaagaaggta ataatcctgt agtcgaaaat gttgtctctc ttgagtggat
      181 cctgagtacg acggagcacg tgaaattccg tcggaatctg ggaggaccat ctcctaaggc
      241 taaatactct ctagtgaccg atagtgaacc agtaccgtga gggaaaggtg aaaagcaccc
      301 cggaagggga gtgaaataga acctgaaacc gtgtgcttac aagtagtcag agcccgttaa
      361 tgggtgatgg cgtgcctttt gtagaatgaa ccggcgagtt acgatttgat gcaaggttaa
      421 gcagtaaatg tggagccgta gcgaaagcga gtctgaatag ggcgtttagt atttggtcgt
      481 agacccgaaa ccaggtgatc tacccttggt caggttgaag ttcaggtaac actgaatgga
      541 ggaccgaacc gacttacgtt gaaaagtgag cggatgaact gagggtagcg gagaaattcc
      601 aatcgaacct ggagatagct ggttctctcc gaaatagctt tagggctagc ctcaagtgat
      661 gattattgga ggtagagcac tgtttggacg aggggcccct ctcgggttac cgaattcaga
      721 caaactccga atgccaatta atttaacttg ggagtcagaa catgggtgat aaggtccgtg
      781 ttcgaaaggg aaacagccca gaccaccagc taaggtccca aaatatatgt taagtggaaa
      841 aggatgtggc gttgcccaga caactaggat gttggcttag aagcagccat catttaaaga
      901 gtgcgtaata gctcactagt cgagtgacac tgcgccgaaa atgtaccggg gctaaacata
      961 ttaccgaagc tgtggattgt cctttggaca atg
//
LOCUS       DAHHOJ010000234          929 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.246, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000234 DAHHOJ010000000
VERSION     DAHHOJ010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..929
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.246"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>929
                     /locus_tag="NBH32_002726"
     rRNA            <1..>929
                     /locus_tag="NBH32_002726"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          247 a          195 c          286 g          201 t
ORIGIN      
        1 atgtggagcg cttagaagtg agaatgccgg tgtgagtagc gaaagacggg tgagaatccc
       61 gtccaccgat tgactaaggt ttccagagga aggctcgtcc gctctgggtt agtcgggtcc
      121 taagctgagg ccgacaggcg taggcgatgg ataacaggtt gatattcctg taccacctat
      181 aatcgtttta atcgatgggg ggacgcagta ggataggcga agcgtgcgat tggattgcac
      241 gtctaagcag taaggctgag tattaggcaa atccggtact cgttaaggct gagctgtgat
      301 ggggagaaga cattgtgtct tcgagtcgtt gatttcacac tgccgagaaa agcctctaga
      361 tagaaaatag gtgcccgtac cgcaaaccga cacaggtagt caagatgaga attctaaggt
      421 gagcgagcga actctcgtta aggaactcgg caaaatgacc ccgtaacttc gggagaaggg
      481 gtgctcttta gggttaacgc ccagaagagc cgcagtgaat aggcccaagc gactgtttat
      541 caaaaacaca ggtctctgct aaaccgtaag gtgatgtata ggggctgacg cctgcccggt
      601 gctggaaggt taagaggagt ggttagcttc tgcgaagcta cgaatcgaag ccccagtaaa
      661 cggcggccgt aactataacg gtcctaaggt agcgaaattc cttgtcgggt aagttccgac
      721 ccgcacgaaa ggcgtaacga tttgggcact gtctcaacga gagactcggt gaaatcatag
      781 tacctgtgaa gatgcaggtt acccgcgaca ggacggaaag accccgtgga gctttactgt
      841 agcctgatat tgaaattcgg cacagcttgt acaggatagg taggagcctt tgaaacgtga
      901 gcgctagctt acgtggaggc gctggtggg
//
LOCUS       DAHHOJ010000235          878 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.249, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000235 DAHHOJ010000000
VERSION     DAHHOJ010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 878)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 878)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..878
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.249"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            88..816
                     /locus_tag="NBH32_002727"
     CDS             88..816
                     /locus_tag="NBH32_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5067 domain-containing protein"
                     /protein_id="HCD1516253.1"
                     /translation="MKKVMGILLASTLILGACGHHQDSAKKESTSHKKKENDNEELNE
                     ELKEFKSKKNMDIKIKGDTIVSDKFEAKIKEPFIINEKDEKKKYIAFKMEITAKKDDK
                     DLNPSSISHDYINITQDDKNTVNKLRDGYLLSDKKYKDWTEHNQDQIKKGKTAQAMFI
                     YELRGDGNINLNVHKYSEDKTVDSKSFKFSKLKTEDFSHRAETREEVEKKEKEFEEEY
                     KKEQEREKEKEKHKDDDHSGLDEV"
BASE COUNT          409 a           92 c          142 g          235 t
ORIGIN      
        1 aaatatcaac tttgtttaat tactcaaaac gataagtcgt ttagagtttt taaaataaat
       61 ttttgcgaaa taaaggagac atgttaaatg aaaaaagtaa tggggatatt attagcaagt
      121 acacttatct taggtgcttg tggacatcat caagatagtg caaaaaaaga gagcactagt
      181 cacaaaaaga aagaaaatga caatgaagaa ttaaatgaag aacttaaaga atttaaaagc
      241 aaaaaaaata tggatataaa aattaaaggc gatactattg ttagtgacaa atttgaagct
      301 aaaataaaag aaccgtttat catcaatgaa aaagatgaga aaaagaaata tatcgctttt
      361 aaaatggaaa ttactgctaa aaaagacgat aaagatttaa atccatcttc tatttctcat
      421 gactatatta atatcactca agatgataaa aatacagtaa ataaattaag agatggttat
      481 cttttaagtg ataaaaaata taaagattgg acagaacata accaagatca aattaaaaaa
      541 ggcaaaactg cacaagccat gtttatctat gaattaagag gtgatggaaa tattaattta
      601 aatgtccata aatactcaga agataaaaca gttgattcta aatcattcaa atttagtaaa
      661 cttaaaaccg aagatttttc tcatagagcg gaaacaagag aagaagtaga aaagaaagaa
      721 aaagaatttg aagaagagta caaaaaagaa caagaacgag agaaagaaaa agaaaagcat
      781 aaagatgacg accacagtgg tttagatgaa gtataaattt atgaatgctc gcattcaacg
      841 ttataatctt tactgttatt attgtcatta tagattag
//
LOCUS       DAHHOJ010000236          871 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.250, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000236 DAHHOJ010000000
VERSION     DAHHOJ010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 871)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..871
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.250"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..860
                     /locus_tag="NBH32_002728"
     CDS             <1..860
                     /locus_tag="NBH32_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCD1516254.1"
                     /translation="RVLKNISFDIEKGTCIALIGKNGAGKSTLIDILIGNVNANSGEI
                     FDKDKLLQSENRSIMFQKTMFPDQLKVIEIINLYQSFYENPLPLEEIIELTKFDSSQL
                     NQFVNKLSGGQQRLLDFVLSLIGQPQLILLDEPTSTMDIEIREYFWSIIENLKEDNRT
                     ILYTSHYIEEVERMSDKIILIENGEIILNDSTSHIRTNQQSQITLSDEYIRKLKLDKD
                     DLVIQKNHNGTIKIITSNVNDTILYLQQLHINLDDIEIQKVSIVDSYFNNKKQRGSNY
                     DTKLLENRI"
BASE COUNT          352 a          114 c          125 g          280 t
ORIGIN      
        1 agcgtgtttt aaaaaacatt tcatttgata ttgaaaaagg tacctgtatc gcattaatcg
       61 gaaaaaatgg tgctggaaaa tcaacgttaa ttgatatatt aattggtaat gttaatgcta
      121 attctggtga gatatttgat aaagacaagt tattacaaag tgaaaatcgc agtataatgt
      181 tccaaaaaac gatgtttcca gatcaattaa aagttattga gattatcaac ttatatcaat
      241 cattttacga aaatccatta ccattggaag aaataataga actgacgaaa tttgattcta
      301 gtcaactgaa ccaatttgta aataaacttt ctggtggtca acaacgatta ctcgattttg
      361 tattatcttt aatcggacaa ccacaattga tcttattaga tgaaccaaca tcgactatgg
      421 atatagaaat tagagaatat ttttggtcaa ttattgaaaa tttaaaagaa gataatcgaa
      481 cgatactcta tacatcgcac tatattgaag aagtcgaacg tatgtcagac aaaattattc
      541 tcattgaaaa tggagaaata atacttaatg attcaacgtc acatattaga accaatcagc
      601 aatctcagat tacgttatcc gatgaatata taagaaagtt aaaactagat aaagatgatt
      661 tagttattca aaaaaatcat aatggcacta tcaaaattat tacttcaaat gtaaatgata
      721 cgattttata tcttcaacaa cttcatatta atttggatga tattgaaata caaaaagtct
      781 caattgttga ttcatacttc aacaataaaa agcaaagggg atctaattat gatactaagt
      841 tacttgaaaa tcgaatttaa agttataatg c
//
LOCUS       DAHHOJ010000237          840 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.251, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000237 DAHHOJ010000000
VERSION     DAHHOJ010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.251"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(74..>840)
                     /locus_tag="NBH32_002729"
     CDS             complement(74..>840)
                     /locus_tag="NBH32_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCD1516255.1"
                     /translation="SYRFIETPFRKEGIKALNWRPSYIPQFIRMAIVVTLLIPFMLIL
                     VGAFNKYGKDIIGEKANSFDTTIEDNYLMRIAPIDNIHIDGLVSEKKKESSDVYNNIK
                     PLLIGDSVMVDIGESFKSSVPKSRIDGKVGRQLYQTLPLVKANYSQYKKSSDQVVLEL
                     GTNGDFTVKQLDDLLNQFGKAKIYLVNTRVPRIYEANVNRLLADAAKRKSNVTLIDWY
                     KRSQGHSEYFAPDGVHLEYKGVLALKDEILKALKKK"
BASE COUNT          267 a          144 c          115 g          314 t
ORIGIN      
        1 gtaaataatt ttttgaatta aaaaaatcta gctaatgtta cgttacacat taactagact
       61 tattaaacat atgttatttc tttttaagtg cttttaatat ttcatctttt aaagctagga
      121 ctcctttgta ctctaaatgt acaccgtctg gtgcaaaata ttcactatgt ccttgtgatc
      181 gcttatacca atcaattaat gtgacattgg actttcgttt cgctgcgtca gctaataatc
      241 gatttacatt tgcctcataa attcttggaa cacgtgtatt aactaaataa atcttggctt
      301 ttccaaattg atttagtaaa tcgtcgagct gtttgacagt aaagtcgcca tttgtaccta
      361 attctaatac gacttgatca gatgattttt tatattgtga ataattcgct ttaactaaag
      421 gtaaggtttg atacaattga cgccctactt ttccatcaat tctagactta ggaactgatg
      481 acttaaatga ctcaccgata tcaaccatta ctgaatcacc gattaaaaga ggtttaatat
      541 tattatatac gtcggaagat tcctttttct tctcacttac taagccatca atgtgtatgt
      601 tatcaattgg tgctatccgc attaaataat tatcttcaat agtggtatca aagctattcg
      661 ctttttctcc aataatgtct ttaccatatt tattgaatgc acctactaaa atcaacataa
      721 atggaattaa caaggttact acaattgcca ttcttataaa ttgtggtata taagaaggtc
      781 gccaatttaa agctttaata ccttcttttc taaatggagt ttctataaag cgatatgata
//
LOCUS       DAHHOJ010000238          839 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.252, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000238 DAHHOJ010000000
VERSION     DAHHOJ010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 839)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 839)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..839
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.252"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..472)
                     /locus_tag="NBH32_002730"
     CDS             complement(<1..472)
                     /locus_tag="NBH32_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HCD1516256.1"
                     /translation="MKINTEFKGNNIPYEYAAGADISDLINGNPIKSFPFEITELPKD
                     TKYLAWSLIDYDAIPVCGFAWIHWSVANVSVTRDSISIKPDLSRTKGDYVQGKNSFTS
                     GLLADDFSEIENHYVGPTPPDQDHQYELTVYALDHSLNLKNGFYLNEFLKEVNQH"
     gene            complement(450..>839)
                     /locus_tag="NBH32_002731"
     CDS             complement(450..>839)
                     /locus_tag="NBH32_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001610400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516257.1"
                     /translation="MGYISSRYLTRYLNYKFLSEICLVIFLIIYTFQNIIAITIAILF
                     LGISSGLTRPQTINELSNSSNLAAILNYAETLYFIFNIAFLLIGGYLYSIGTIQYLML
                     FMSLLTFIYLLTLFYLRRDQHENQHRI"
BASE COUNT          302 a          125 c          120 g          292 t
ORIGIN      
        1 tatgttgatt tacttctttt aaaaattcat tcaagtagaa cccattcttc aaatttaaag
       61 aatgatctaa cgcataaact gttaattcat attgatgatc ttgatcaggt ggtgtaggtc
      121 ctacatagtg attttctatt tctgaaaaat catcagctaa caaaccacta gtgaagctat
      181 ttttaccttg tacatagtcg ccctttgttc ttgataaatc aggtttaatt gaaattgaat
      241 cacgagtgac acttacatta gcaacgctcc aatgaatcca tgcaaatccg caaactggta
      301 ttgcatcata atctatcaga gaccatgcaa gatatttagt atcttttgga agttctgtta
      361 tttcaaacgg gaatgattta attggattgc catttattaa atcacttata tctgcgcctg
      421 ccgcatattc atatggtata ttatttcctt taaattctgt gttgattttc atgttgatct
      481 ctccttaaat aaaataatgt tagtaaatat ataaacgtca ataatgacat aaataacatt
      541 aaatattgaa tagttccaat tgaatatagg taaccaccaa taagtaaaaa tgctatatta
      601 aaaataaagt acagtgtttc cgcgtaatta agtatagctg ctaagttact actatttgaa
      661 agttcattaa ttgtctgagg acgagttaag cctgaagaaa tccctaaaaa taaaatagcg
      721 attgtaattg caataatatt ttggaatgta tatattatta aaaatattac taaacaaatt
      781 tcagatagaa atttataatt caaatatcta gtcaagtagc gggaagaaat gtaacccat
//
LOCUS       DAHHOJ010000239          795 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.254, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000239 DAHHOJ010000000
VERSION     DAHHOJ010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.254"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            1..76
                     /locus_tag="NBH32_002732"
                     /pseudo
     CDS             1..76
                     /locus_tag="NBH32_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001622381.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
     gene            503..751
                     /locus_tag="NBH32_002733"
     CDS             503..751
                     /locus_tag="NBH32_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516258.1"
                     /translation="MLILKIKEINDKSVTYKYFPNNDENIKPGIIQMDIDSLEVINAE
                     KSSLEKNTRDNYFIHAIDRIYINASKGLFPESELVAWG"
BASE COUNT          341 a           80 c          129 g          245 t
ORIGIN      
        1 aagtaccaca gtgccacatg tcggcaattg gcgagacaag ttctttaaag agcgtgaagg
       61 taaatatcaa gtagaaggtt gttttattga atcaggtgca cttaataata aaagtgatga
      121 atacggtatc aaaaggaaca gacatgctca aatttattat aattcagtta gaaatcgaga
      181 taaacagata gagatttcta aaattgctaa aaatactaat ataaataaaa atttaattca
      241 aagagtttat gaacatatat ttgaaaataa gtatttatta gaaagtggat ttaaacaatt
      301 tgatcctgac ttttatatgg ctcaaagttg gcaacgttta agagaaggga aaaatattaa
      361 aaaaatggat ataataacgt agaaacatga agctcttgaa cactatctaa tgaataagta
      421 taatttacac tatatagagg cacataaact tacagaaata aaatataatt atagtatttt
      481 aattaattag aggtggaaat gattgctaat attaaaaata aaagaaatta atgataaaag
      541 tgttacatat aaatattttc ctaataatga tgaaaatatt aagcctggta ttattcaaat
      601 ggatatagat agtcttgaag ttatcaatgc tgaaaaatct agtttagaaa aaaatacaag
      661 agataattat tttattcatg ccatagacag aatatacata aatgctagta aagggctatt
      721 tcctgaatct gaactcgtag catggggata aagaaagttt actttaaaaa ttttaacctt
      781 caaatgtgaa ggtta
//
LOCUS       DAHHOJ010000240          676 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.255, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000240 DAHHOJ010000000
VERSION     DAHHOJ010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..676
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.255"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            146..643
                     /locus_tag="NBH32_002734"
     CDS             146..643
                     /locus_tag="NBH32_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516259.1"
                     /translation="MSLFDRLSVLKNKSEIKLIESKQVVEDKVFEIRHGKLDDIPMTK
                     ERKRSEKTRKKLEKHESKVKEKEKRKQQREEEIEKRRIAKEEQEKRDVWIKENVFKII
                     VNEEGKRIIFCPKCGSENNVTATTWNVDEAENSEDIGTYFKRTCNLCGHSQKFNKDNP
                     KKIYE"
BASE COUNT          316 a           49 c          128 g          183 t
ORIGIN      
        1 tagtcatata tattggataa tagaaggaaa tacgtatggt tataataata taacaaatgg
       61 gttaaattta attaacgaat aatagctaaa atctgataag taaatggtat aatatttgta
      121 aatataaatt gtttggaggt atcatatgtc tttatttgat agactaagtg ttctaaaaaa
      181 taaatcagaa ataaaactta tagaaagtaa gcaagtagtt gaagataaag tatttgaaat
      241 acgacatggt aaattagatg atataccaat gactaaagaa agaaagcgtt cagaaaaaac
      301 gaggaaaaaa cttgaaaaac atgaatcaaa ggtaaaagaa aaagaaaaaa gaaagcaaca
      361 aagagaagaa gaaatagaaa aaaggagaat agcaaaagaa gagcaagaaa agcgtgatgt
      421 ttggattaaa gagaatgtat ttaaaattat agtaaatgag gaaggtaaac gaataatttt
      481 ttgtcctaaa tgtggtagtg agaataatgt aactgctact acttggaacg tagatgaagc
      541 tgaaaatagt gaggatattg gaacatattt taagagaact tgtaatttat gcggacattc
      601 tcaaaaattt aataaagata accctaaaaa aatttatgaa taggaataaa gaattgatac
      661 attttctgga taagtg
//
LOCUS       DAHHOJ010000241          663 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.256, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000241 DAHHOJ010000000
VERSION     DAHHOJ010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 663)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 663)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..663
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.256"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>663
                     /locus_tag="NBH32_002735"
     rRNA            <1..>663
                     /locus_tag="NBH32_002735"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          162 a          145 c          211 g          145 t
ORIGIN      
        1 cgtggtggga gacagtgtca ggcgggcagt ttgactgggg cggtcgcctc ctaaaaggta
       61 acggaggcgc tcaaaggttc cctcagaatg gttggaaatc attcatagag tgtaaaggca
      121 taagggagct tgactgcgag acctacaagt cgagcagggt cgaaagacgg acttagtgat
      181 ccggtggttc cgcatggaag ggccatcgct caacggataa aagctacccc ggggataaca
      241 ggcttatctc ccccaagagt tcacatcgac ggggaggttt ggcacctcga tgtcggctca
      301 tcgcatcctg gggctgtagt cggtcccaag ggttgggctg ttcgcccatt aaagcggtac
      361 gcgagctggg ttcagaacgt cgtgagacag ttcggtccct atccgtcgtg ggcgtaggaa
      421 atttgagagg agctgtcctt agtacgagag gaccgggatg gacatacctc tggtgtacca
      481 gttgtcgtgc caacggcata gctgggtagc tatgtgtgga cgggataagt gctgaaagca
      541 tctaagcatg aagcccccct caagatgaga tttcccaact tcggttataa gatccctcaa
      601 agatgatgag gttaataggt tcgaggtgga agcatggtga catgtggagc tgacgaatac
      661 taa
//
LOCUS       DAHHOJ010000242          653 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.257, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000242 DAHHOJ010000000
VERSION     DAHHOJ010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.257"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..438)
                     /gene="sem"
                     /locus_tag="NBH32_002736"
     CDS             complement(<1..438)
                     /gene="sem"
                     /locus_tag="NBH32_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_058008129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="staphylococcal enterotoxin type M"
                     /protein_id="HCD1516260.1"
                     /translation="MKRILIIVVLLFCYSQNHIATADVGVLNLRNYYGSYPIEDHQSI
                     NPENNHLSHQLVFSMDNSTVTAEFKNVDDVKKFKNHAVDVYGLSYSGYCLKNKYIYGG
                     VTLAGDYLEKSRRIPINLWVNGEHQTISTDKVSTNKKLVTAQEI"
BASE COUNT          212 a          109 c           74 g          258 t
ORIGIN      
        1 aatttcttga gctgttacta actttttatt agttgatact ttgtcagtag atatagtttg
       61 atgttctcca ttaacccaaa gattaatagg aatacgtcta gatttctcta aataatcacc
      121 tgctaatgta actccaccgt atatatattt gtttttcaaa caatatccac tataacttag
      181 accatataca tctacagcat gatttttgaa tttctttaca tcatcaacgt tcttaaattc
      241 agctgttact gtcgaattat ccatagaaaa aactaattga tgcgaaagat gattattttc
      301 aggattaata ctttggtggt cttcaattgg atagctacca taatagttcc taagattcaa
      361 aactccgaca tcagcggttg cgatatgatt ttgcgaatag caaaacaata aaacaacaat
      421 gataagtatt cttttcatat tttatttctc ctatatttaa acttctctat ctttattatc
      481 ttttattgta aaaatagcca attcttctta aatcatcatg attctatccc acttatatat
      541 tattactcgt taatctggtt ttgttatcta tactaaaata gaaagctttt tatagtaata
      601 aagttagatt tgaatcacta ttcgttcttc atcatttact aagctacttt gta
//
LOCUS       DAHHOJ010000243          647 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.258, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000243 DAHHOJ010000000
VERSION     DAHHOJ010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 647)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..647
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.258"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            246..493
                     /locus_tag="NBH32_002737"
                     /pseudo
     CDS             246..493
                     /locus_tag="NBH32_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000239616.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sphingomyelin phosphodiesterase"
BASE COUNT          250 a           85 c           82 g          230 t
ORIGIN      
        1 gattaactgc taaattctta gttgcaaaaa agttgtttgc ttttatggat gttaagcctt
       61 aaatgtaaat ggcttactcg cctctaaccc ttaattccga ctttatttat ctaatatgtt
      121 tacattgtct taatatataa aaagcatttg cttaaaaata taaattcgat ttaataatta
      181 atttaatttt agttaatcaa ttttgtgact attttattgt aagctatata aaaggagtga
      241 taatgatggt gaaaaaaaca aaatccaatt cactaaaaaa agttgcaaca cttgcattag
      301 caaatttatt attagttggt gcacttactg acaatagtgc caaagcagaa actaagaaag
      361 atgatactga tttaaaatta gtcagtcata acgtttatat gttatcgacc gttttgtatc
      421 caaactggag acttttaaca taaaattact tatcattcaa aaagtaaaac agcataatat
      481 caaggtttat aactttatca ttatcaataa tacctcatat aaaataaaat tttagggact
      541 ttttagggac tttaaattta aaattacaag tttaatagaa acatcaaaat aatcacatgt
      601 ttgtgtggaa tgtacacccc aaaagctaga ctgaaaaatc tattttt
//
LOCUS       DAHHOJ010000244          587 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.260, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000244 DAHHOJ010000000
VERSION     DAHHOJ010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..587
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.260"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            1..587
                     /locus_tag="NBH32_002738"
                     /pseudo
     CDS             1..587
                     /locus_tag="NBH32_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
BASE COUNT          241 a           80 c           89 g          177 t
ORIGIN      
        1 atttgatgat tattctgaaa gaaaaagtaa atatgaagag caaaaatcga ttcttaaaga
       61 tagaaatagc ttttctaaaa ctgatcatga tgcaactttt atgagaatga aagaagacca
      121 tatgaaaaat ggccaactta agccaggata caatttacaa atagcgacaa attctcaatt
      181 tgttttatcc tatgacctat ttcaaaatcc aacagatact ggaacattaa ttccattttc
      241 aacactcaaa attggaaata tgacgaactt aatgatgaat ttatatgtcc taataacaaa
      301 agaataggtt ttaaaagata tgcataccat aatgatagat atggttttaa acgagacttc
      361 aaactatatg aatgtgatga ctgttcatca tgttctttga gacatcaatg catgaagcca
      421 aattcgaaat ccaataagaa aattatgaag aattataatt gggaatactt taaagcccaa
      481 attaatcaaa agcgttctga accagaaacg aaaaaaatct atagtcaaag aaaaattgat
      541 gtagagcctg tttttggatt tatgaaagct attttgagtt tcattcg
//
LOCUS       DAHHOJ010000245          583 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.261, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000245 DAHHOJ010000000
VERSION     DAHHOJ010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..583
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.261"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..100)
                     /locus_tag="NBH32_002739"
     CDS             complement(<1..100)
                     /locus_tag="NBH32_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HCD1516261.1"
                     /translation="MKNKLIAKSLLAIAAIGITTTTIASTADASEGY"
     gene            complement(359..>583)
                     /locus_tag="NBH32_002740"
     CDS             complement(359..>583)
                     /locus_tag="NBH32_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516262.1"
                     /translation="VDLTEVVVLEHLIQWFLVYFAIYQAVNEKMDINDINIDNFQSVF
                     FDVSNLNLVILPTLIISWVTIFNYRMRSYK"
BASE COUNT          206 a          100 c           66 g          211 t
ORIGIN      
        1 cgtatccttc gctcgcatct gctgttgacg caattgtagt tgtagtaata ccaattgccg
       61 ctattgctaa taaagatttt gctatcaatt tatttttcat gttaattatc ctccaaatca
      121 ttatcttaca tttattaagt tatcctattt atacaacttt tgaaattaac taattattaa
      181 ttaactataa acactttata atatagacaa aatatagcta tctatcttgt gactcatcag
      241 cacttataca ctagtcaaaa aattcatgcc tctacttttt cgaatttcaa aaataaaacc
      301 acacaacaaa aaagacacca tttgaatgtt ggtgtctgat ggtataattt catagatttt
      361 atttgtaact tctcattcta tagttaaata ttgtgaccca gctaatgatt aacgttggta
      421 gaattactaa attcaaatta gacacgtcaa aaaagacaga ttggaaattg tcgatattaa
      481 tatcattaat gtccattttt tcatttactg cttgatagat cgcaaagtaa actaagaacc
      541 attgaattaa atgttctaaa acgacaacct ctgtcaaatc tac
//
LOCUS       DAHHOJ010000246          573 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.262, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000246 DAHHOJ010000000
VERSION     DAHHOJ010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.262"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
BASE COUNT          171 a          107 c           96 g          199 t
ORIGIN      
        1 aattaatact tattacaagt acatcatata ttagttgata acgattatca atgtcgcgtg
       61 gatttgtgac acatttcttt taaaaattca caaggttatg gggcagaaat gataaagagc
      121 cactaatgat ttattatgta atgattcttt atccttagcc acagctaata tgtacttaaa
      181 aataggaata catgagtaaa actctatgtt ggggctccgc caacttgcac attattgtaa
      241 gctgactttc cgccagctac tgtgttgggg gcccgccaac ttgcacatta ttgtaagctg
      301 actttccgcc agctactgtg ttgggggccc gccaacttgc attgtctgta gaaactgtgg
      361 atccaatttc tctatgttgg gtccctgagt atagccttgt agagtctagt acattgattt
      421 gtatcccaat gtccctataa ttgattattc gctttatcta atgatcctat gactcaacta
      481 ttaaatcatt tttcgaaata cttaattcta aaataattaa attcatttat tgtaatattg
      541 caaaaaatac attgcacacc ttgttcatca atg
//
LOCUS       DAHHOJ010000247          549 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.263, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000247 DAHHOJ010000000
VERSION     DAHHOJ010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..549
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.263"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..414)
                     /locus_tag="NBH32_002741"
     CDS             complement(<1..414)
                     /locus_tag="NBH32_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLDc N-terminal domain-containing protein"
                     /protein_id="HCD1516263.1"
                     /translation="MQFSFSNDLGTLFTIILAIAFIINLVLAFIIIFLERNRRTASST
                     WAWLFVLFVLPLIGFILYLFFGRTVSARKLNKNNGNVLTDFDALLKQQIESFDKGNYG
                     TDNKQVQKHHDLVRMLLMDQDGFLTENNKIDHFIDG"
BASE COUNT          199 a           93 c           64 g          193 t
ORIGIN      
        1 tccgtcaata aaatgatcaa ttttattatt ttcagttaaa aagccatctt gatccattaa
       61 caacatacgt accaaatcat ggtgcttttg aacttgttta ttatctgtac cataattacc
      121 tttatcaaag ctttcaatct gctgctttaa aagcgcatca aaatctgtta acacgttccc
      181 attattttta tttagtttac gcgcagaaac cgttcttcca aaaaataaat aaaggataaa
      241 accaatcaat ggtaagacaa aaagtacaaa taaccacgcc catgttgaac tggctgtacg
      301 tctatttctt tctaaaaaaa taataataaa cgctagaact aaattaatga taaaggcaat
      361 tgctaaaatg atggtaaaaa gtgttcctaa atcatttgaa aatgaaaatt gcatagttta
      421 tttctcctat aaagaaaggc acaaaaaact tttgtacctt tctttccaga ttatttattt
      481 acagatagag ttaatattgc attaaactct tcttcattat ttggaaatgt tctttcttca
      541 atttctttt
//
LOCUS       DAHHOJ010000248          508 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.265, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000248 DAHHOJ010000000
VERSION     DAHHOJ010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.265"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(390..504)
                     /gene="rrf"
                     /locus_tag="NBH32_002742"
     rRNA            complement(390..504)
                     /gene="rrf"
                     /locus_tag="NBH32_002742"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          116 a          126 c           92 g          174 t
ORIGIN      
        1 cgattaggta ttgaaacgac acgctttttt catattcttg aaacatatat agcagtacat
       61 ctatgtctac tttgaaggga gcatttcaat gcggctcatc gcatccattt tttgcctggc
      121 aacgttctac tctagcggaa cgtaagttgg ctaccatcct cgctaagaac ctttcttgac
      181 ttgtgacaat cgcttgcttc tttcctcttc ttcggctctc gcttactcat ttagctctac
      241 taaactcgtt gcgctctttt ctcgtttcgt cagattcaaa cgttttcact tcgccaagcc
      301 atttttcttt gtgttaactt ttattttgac gttttagaca taaaaaaaga gaccttacgg
      361 tctcaatgcg gctcatcgca tccatttttg cctggcaacg ttctactcta gcggaacgta
      421 agttcgacta ccatcgacgc taaggagctt aacttctgtg ttcggcatgg gaacaggtgt
      481 gacctccttg ctatagtcac cagacata
//
LOCUS       DAHHOJ010000249          503 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.266, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000249 DAHHOJ010000000
VERSION     DAHHOJ010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 503)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 503)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..503
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.266"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>503
                     /locus_tag="NBH32_002743"
     CDS             <1..>503
                     /locus_tag="NBH32_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCD1516264.1"
                     /translation="LSFSLLTFPLDIINEKQNEWRQRLMVTPFTFTSYYISKVVKTML
                     QFAIAILVIFMVGHFYKGVAMSAVQWLESGIFLWLGASLLITFGILFSLLNDIQKTSA
                     LANIVTIGLAVLGGLWFPINTFPNWLQHVAHVLPSYHLRKLGVDIASNHHINLISFAI
                     ILLYALG"
BASE COUNT          156 a           64 c           86 g          197 t
ORIGIN      
        1 tgttaagttt tagtttacta acttttccat tagatattat taatgaaaaa caaaatgaat
       61 ggcgccaaag attaatggta acaccattta cttttactag ttattatatt tcaaaagtag
      121 tgaaaactat gctgcaattt gcaatagcga tattagttat ttttatggtt ggacattttt
      181 ataaaggtgt tgcaatgagt gcagttcaat ggttagagtc aggaatattt ttatggttag
      241 gtgcgtctct attaataact tttggcatat tattttcttt gttaaatgat attcaaaaaa
      301 caagtgcttt agctaatatc gtaacaattg gtttagcagt attaggtgga ttgtggtttc
      361 cgataaacac atttccaaat tggcttcaac atgttgctca tgttttaccg agctatcatt
      421 tgcgtaaact aggtgtagat attgcttcaa atcatcatat caatttaata tcatttgcta
      481 taatactctt gtatgcttta ggg
//
LOCUS       DAHHOJ010000250          492 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.267, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000250 DAHHOJ010000000
VERSION     DAHHOJ010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..492
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.267"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            1..492
                     /locus_tag="NBH32_002744"
                     /pseudo
     CDS             1..492
                     /locus_tag="NBH32_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          204 a           69 c           73 g          146 t
ORIGIN      
        1 acgaagattg tgctcaaatt agaaaacaga cctgtaaaaa agaactgaga ttaagaagtt
       61 caaaaagaaa tttgatgatt attccgaaag aaaaaataaa tatgaagaac aaaaatcgat
      121 tcttaaagat agaaatagct tttctaaaac tgatcatgat gcaactttta tgagaatgaa
      181 ggaagaccat atgaaaaatg gccaacttaa gccaggatac aatttacaaa tagcgacaaa
      241 ttctcaattt tttatcctat gacctatttc aaaccccaac agatactaga actttaattc
      301 catttttaac aatgattcaa aataccttcg gttatttacc agagtatatt gtagctgatg
      361 caggttatgg tagtgagcaa aactatatgg ctattataga tgattttaat aaaacgccac
      421 ttattacgta tggcatgttt attaaagata aaactagaaa gtttaaaagt gacattttta
      481 acactcaaaa tt
//
LOCUS       DAHHOJ010000251          471 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.268, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000251 DAHHOJ010000000
VERSION     DAHHOJ010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 471)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 471)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..471
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.268"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(135..449)
                     /locus_tag="NBH32_002745"
     CDS             complement(135..449)
                     /locus_tag="NBH32_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516265.1"
                     /translation="MIGQTNLFDDVLKQDERFVIVVQALEEKNGQLLKRTLREYPGLN
                     HKQMNDLFTHLKELFSEESFAENQSAFSITVYTNLDYTADQIYAHVKRFRGKHDWTQT
                     AK"
BASE COUNT          154 a           74 c           66 g          177 t
ORIGIN      
        1 ttaataaatc aagtattaat tagtcacgga aggtagataa ataaatttga atcattctga
       61 ataatgacga atgtattaaa aaatgcagtg acaatctaac attaattgtt aaaatcactg
      121 caagcatctt gattttattt agctgtttgt gtccagtcat gcttccctct gaagcgtttt
      181 acatgagcat atatttggtc agcagtataa tctaaatttg tataaactgt aatactgaac
      241 gctgattggt tttcagcaaa tgattcttcg gaaaataatt cctttaagtg cgtaaataaa
      301 tcattcattt gtttatggtt taaaccggga tattccctta aagttctttt taataattgt
      361 ccgttctttt cttctaatgc ttgaactaca ataacaaatc tctcatcttg ctttagtaca
      421 tcatcaaata aatttgtttg tccaatcatt gaatttcacc cctgaaaagt t
//
LOCUS       DAHHOJ010000252          452 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.269, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000252 DAHHOJ010000000
VERSION     DAHHOJ010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.269"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>452
                     /locus_tag="NBH32_002746"
     CDS             <1..>452
                     /locus_tag="NBH32_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1516266.1"
                     /translation="EQKFYYKRILWQYLKDKHGLTCAYSTFRAYILKHDEFNRYFMKG
                     YQQMSPKGTTRFETKAGHQAQFDWKEGINFKTKDNQIVSLNIGVLLLPYSRFVIMQVT
                     MNKSSDVLFNLLTQAFELMGGVPNELVTDNMKTVMDQPRTERTNGQINR"
BASE COUNT          161 a           73 c           87 g          131 t
ORIGIN      
        1 gaacaaaagt tctactataa gcgtattctt tggcaatatt tgaaggataa acatgggtta
       61 acgtgtgcct attctacatt tagagcttat atccttaaac atgatgagtt taatcgttat
      121 tttatgaaag gttatcaaca gatgtcgcct aaaggcacaa cgagattcga gacaaaagcc
      181 ggtcatcaag cacagtttga ctggaaagaa ggtattaact ttaaaacgaa ggataatcaa
      241 atcgtttcac taaatatagg cgtactacta cttccttatt cccgttttgt aatcatgcaa
      301 gtgacaatga ataaatcaag tgatgtctta tttaatttat tgacacaagc gtttgagcta
      361 atgggaggtg tgcctaatga acttgtaacg gataacatga aaacggtcat ggatcaacca
      421 agaacagaac gtacaaatgg tcaaataaat cg
//
LOCUS       DAHHOJ010000253          452 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.270, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000253 DAHHOJ010000000
VERSION     DAHHOJ010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..452
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.270"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>452
                     /locus_tag="NBH32_002747"
     CDS             <1..>452
                     /locus_tag="NBH32_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000777469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1516267.1"
                     /translation="EQKFYYKRILWQYLKDKHGLMCAYSTFRAYILKHDEFNRYFMKG
                     YQQMSPKGTPRFETKAGHQAQFDWKEGINFKTKDNQIVSLNIGVLLLPYSRFVIMQVT
                     MNKSSDVLFNLLTQAFELMGGVPNELVTDNMKTVMDQPRTERTNGQINR"
BASE COUNT          161 a           75 c           86 g          130 t
ORIGIN      
        1 gaacaaaagt tctactataa gcgtattctt tggcaatatt tgaaggataa acatgggtta
       61 atgtgtgcct attctacatt tagagcttat atccttaaac atgatgagtt taatcgttat
      121 tttatgaaag gttatcaaca gatgtcgcct aaaggcacac cgagattcga gacaaaagcc
      181 ggtcatcaag cacagtttga ctggaaagaa ggcattaact ttaaaacgaa ggataatcaa
      241 atcgtttcac taaatatagg cgtactacta cttccttatt cccgctttgt aatcatgcaa
      301 gtgacaatga ataaatcaag tgatgtctta tttaatttat tgacacaagc gtttgagcta
      361 atgggaggtg tgcctaatga acttgtaaca gataacatga aaacggtcat ggatcaacca
      421 agaacagaac gtacaaatgg tcaaataaat cg
//
LOCUS       DAHHOJ010000254          435 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.271, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000254 DAHHOJ010000000
VERSION     DAHHOJ010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..435
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.271"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            316..435
                     /locus_tag="NBH32_002748"
                     /pseudo
     CDS             316..435
                     /locus_tag="NBH32_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501277.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
BASE COUNT          173 a           41 c           79 g          142 t
ORIGIN      
        1 aaaacttaaa taatataaaa tataaatatt aatatgagta tttttgcagc atgcaaatgg
       61 cattgctaca tttattaaaa tgcgcaattt gttatgagtg acttttttga atagaaggca
      121 tattacagtg acgaaaacag tttgtgtgga agacagggaa aattctgctt atttaaaatg
      181 atagtttgta ttatagatgg atttgtgagg tgggtttgcg gaataaatgg tgcataacta
      241 agtaaatata tgttaattac aacaatattt gacaaaaatg ggagtgacaa caataatata
      301 gttaaccggt taactaatga taaagtgaat aataaatttt tgtctgaaat gttaaatgta
      361 tcaaaaccag caattactaa atcgataaaa aaacttttag cgaaaggctt agtagttgaa
      421 tcgcacaatg aattt
//
LOCUS       DAHHOJ010000255          425 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.273, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000255 DAHHOJ010000000
VERSION     DAHHOJ010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.273"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            74..425
                     /locus_tag="NBH32_002749"
                     /pseudo
     CDS             74..425
                     /locus_tag="NBH32_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          162 a           70 c           53 g          140 t
ORIGIN      
        1 aaatacttta aaaaaataag acactttcgc ataatttaat aaataccact aaactaaatt
       61 aacgaggtgc cttatgtata aaaattataa catgacccaa cttacactac caatagaaac
      121 ttctgttaga attcctcaaa atgatatttc gcgatatgtt aatgaaattt ttgaaacgat
      181 acctgatagc gaattcgatg aattcagaca tcatcgtggc gcaacatcct atcatccaaa
      241 aatgatatta aaaatcatct tatatgcata tactcaatct gtattttctg gtcgtagaat
      301 agagaaatta cttcatgaca gtattcgaat gatgtagtta gctcaagatc aaataccttc
      361 ttataaaact attaatcgtt ttagagtgaa tcctaatact gatgcgttaa ttgaatcttt
      421 attta
//
LOCUS       DAHHOJ010000256          414 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.274, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000256 DAHHOJ010000000
VERSION     DAHHOJ010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.274"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..399
                     /locus_tag="NBH32_002750"
     CDS             <1..399
                     /locus_tag="NBH32_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding protein"
                     /protein_id="HCD1516268.1"
                     /translation="GPREKKPVSINHNIVEYNDGTFKYQSRPKFNSTPKYIKFRHDYN
                     IVEYNDGTFGYGPRPHFNKPAAKTDATIKKEQKLIQAQNLVREFEKTHTVSAHRKAQK
                     AVNLVSFEYNVKKMILQERIDEVLKQGLVR"
BASE COUNT          173 a           60 c           72 g          109 t
ORIGIN      
        1 ggtccaagag aaaagaaacc agtgagtatt aatcacaata tcgtagagta caatgatggt
       61 acttttaaat atcaatctag accaaaattt aactcaacac ctaaatatat taagtttaga
      121 catgattata acattgtgga atataatgac ggtacatttg gatatggacc acgtccgcat
      181 tttaataaac cagcagcgaa aactgatgca actataaaaa aagaacaaaa attgattcaa
      241 gctcaaaatc ttgtgagaga atttgaaaag acccatactg tgagcgcaca tagaaaagca
      301 caaaaggcag tcaacttagt atcatttgaa tacaatgtta agaaaatgat attgcaagag
      361 cgaattgatg aagtattaaa gcaaggatta gtgagataat acttctgtca ttat
//
LOCUS       DAHHOJ010000257          377 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.276, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000257 DAHHOJ010000000
VERSION     DAHHOJ010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 377)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 377)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..377
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.276"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            207..377
                     /locus_tag="NBH32_002751"
                     /pseudo
     CDS             207..377
                     /locus_tag="NBH32_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          112 a           74 c           74 g          117 t
ORIGIN      
        1 cccgtctggc acattactgt aaaattctat aaatagaatt tttgatgacg ggtcccttcc
       61 tagggtgccg tctcagccac cccaaccggc acattgttgt aagctgacta tatgtcagct
      121 tctgtgtggg gccctgtctt cgactggcac tgctccctca ggagtctcgc cattaatact
      181 acgtattaac atgtaatttt actttgaaat actttaaagc ccaaattaat caaaagcttt
      241 ctgaaccaga aacgaaaaaa atctatagtt aaagaaaaat tgatgtagag cctgtttttg
      301 gatttatgaa ggctattttg ggtttcacta gaatgtcagt tcgaggaata aataaagtta
      361 aacgagagct aggtttt
//
LOCUS       DAHHOJ010000258          376 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.277, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000258 DAHHOJ010000000
VERSION     DAHHOJ010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..376
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.277"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            1..117
                     /locus_tag="NBH32_002752"
     CDS             1..117
                     /locus_tag="NBH32_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001789089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCD1516269.1"
                     /translation="MPFSIITLITGIIMHMTMYFVKFECWKMPLFMKIIFTI"
BASE COUNT          158 a           42 c           63 g          113 t
ORIGIN      
        1 ataccctttt ctattattac attaattaca ggtatcatta tgcatatgac aatgtacttt
       61 gtaaaatttg aatgttggaa aatgccatta ttcatgaaga ttatttttac aatctaaaag
      121 cagtaaagac tcataatata gccaaaaacg ttaataaaag tttgttaaat gataaggggg
      181 tgagtattgg taaatttatt caaaaggtta aagggaaaaa tcctacctag agagatccaa
      241 aaactaaatg gactatttcc aaaaataaag ggcaaccaca tggtgattct tattggaagt
      301 taataaataa caaaggaaac agaatagatt ctttaactaa agaaggaaaa aatcttaagg
      361 gaataaggtg ttgtaa
//
LOCUS       DAHHOJ010000259          375 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.278, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000259 DAHHOJ010000000
VERSION     DAHHOJ010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..375
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.278"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(138..350)
                     /locus_tag="NBH32_002753"
                     /pseudo
     CDS             complement(138..350)
                     /locus_tag="NBH32_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="zinc peptidase"
BASE COUNT          133 a           62 c           44 g          136 t
ORIGIN      
        1 atagcctaaa aagcttgtta tatcgcacaa aacacaacat taaaaggtcc ttgtcgatgc
       61 ctatcaacag atgttacaaa taaaaacctc gaactgaaat gatgatcttt tcagctcgag
      121 gtttaaatat tggtgcctta tttatataga ttcgttatat tatattctct attttcatta
      181 acataatcct taaagagttt taaattaata cctgctagat gattcaaaaa tgtttcatca
      241 acttttaaat aattcaataa tttttgtggt gtcagtatat ttctatcaaa atacaacttt
      301 aataaactat tcattttgac aggacgtgac atttcaatca cgtcgtctaa agataatact
      361 ttctcgcttt aaaca
//
LOCUS       DAHHOJ010000260          320 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.283, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000260 DAHHOJ010000000
VERSION     DAHHOJ010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..320
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.283"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(55..>320)
                     /locus_tag="NBH32_002754"
     rRNA            complement(55..>320)
                     /locus_tag="NBH32_002754"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT           79 a           83 c           71 g           87 t
ORIGIN      
        1 atatgtcacg ttattccgca tcttctgaag aagatgttcc gaatatatcc ttagaaagga
       61 ggtgatccag ccgcaccttc cgatacggct accttgttac gacttcaccc caatcatttg
      121 tcccaccttc gacggctagc tcctaaaagg ttactccacc ggcttcgggt gttacaaact
      181 ctcgtggtgt gacgggcggt gtgtacaaga cccgggaacg tattcaccgt agcatgctga
      241 tctacgatta ctagcgattc cagcttcatg tagtcgagtt gcagactaca atccgaactg
      301 agaacaactt tatgggattt
//
LOCUS       DAHHOJ010000261          313 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.284, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000261 DAHHOJ010000000
VERSION     DAHHOJ010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..313
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.284"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>313
                     /gene="tnpA"
                     /locus_tag="NBH32_002755"
     CDS             <1..>313
                     /gene="tnpA"
                     /locus_tag="NBH32_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002458050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS200/IS605-like element ISSep3 family
                     transposase"
                     /protein_id="HCD1516270.1"
                     /translation="GIILRQLCERKGVEIIEAEACKDHIHMLVSIPPKLGVSSFVGYL
                     KGKSSLMIFDRHANLKYRYGNRKFWCKGFYVDTVGRNKKVIENYIRNQLQEDIVADQI
                     S"
BASE COUNT          121 a           29 c           66 g           97 t
ORIGIN      
        1 tagggattat attgcgtcaa ttatgtgaga gaaaaggtgt agagataatt gaagcagagg
       61 catgtaaaga tcatatccat atgttagtaa gtattccacc caaacttggg gtatcatcat
      121 ttgttggcta tttaaaagga aaaagtagtt taatgatatt tgatagacat gctaacttaa
      181 agtatagata tggaaataga aaattttggt gtaaaggatt ttatgtggat acagtaggta
      241 gaaataaaaa agtgattgaa aattatattc gtaatcaatt acaagaggat atcgttgcag
      301 accaaatctc aat
//
LOCUS       DAHHOJ010000262          306 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.286, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000262 DAHHOJ010000000
VERSION     DAHHOJ010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..306
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.286"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            178..306
                     /locus_tag="NBH32_002756"
                     /pseudo
     CDS             178..306
                     /locus_tag="NBH32_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499365.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          117 a           26 c           51 g          112 t
ORIGIN      
        1 atttgttaag aaattgcgtt tataaaaata aaaaaatcaa cttatttgta tgagatgaat
       61 atgtattgaa gaagacgtgt tattaatttg gaaaatactg gtcaaagatg gaagctctca
      121 aaagcgttat tgtattcttt agtcaatgca aatagattgc cgtaataata atcgtacttg
      181 atggttaaaa aattacttaa ggctttaaag caaaactttt tatatgagta gtcgaatata
      241 acgtttaaaa tgattgtttt tggatataaa cgattaagta aaatgctttt tcagtttgta
      301 attaat
//
LOCUS       DAHHOJ010000263          295 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.289, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000263 DAHHOJ010000000
VERSION     DAHHOJ010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..295
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.289"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..>295)
                     /locus_tag="NBH32_002757"
     CDS             complement(<1..>295)
                     /locus_tag="NBH32_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="beta-lactam sensor/signal transducer BlaR1"
                     /protein_id="HCD1516271.1"
                     /translation="FNNLNNQEPTVESNSHNLNPNINTTKPVHEFTTDIHKFNWDSID
                     NICTVIWIVLVIILSFKFLNSLLYLKYLKKQSLYLNEKEKDKINKILFNHQYK"
BASE COUNT          103 a           31 c           37 g          124 t
ORIGIN      
        1 cttttatatt gatgattgaa aagtatcttg tttattttat ctttttcttt ttcatttaga
       61 taaagtgact gtttctttaa atacttaaga tataataaag aatttaaaaa tttaaaactt
      121 aaaataataa ctaaaactat ccaaataact gtgcagatat tatctattga atcccaatta
      181 aacttatgga tgtctgttgt aaactcatga acaggtttag tggtatttat gttaggattc
      241 aaattgtgtg aattactttc aactgtgggc tcttgattat tcaaattatt aaatt
//
LOCUS       DAHHOJ010000264          269 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.291, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000264 DAHHOJ010000000
VERSION     DAHHOJ010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.291"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>269
                     /locus_tag="NBH32_002758"
     CDS             <1..>269
                     /locus_tag="NBH32_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HCD1516272.1"
                     /translation="NIINNWLVLFIPLLNWLTPKRYIKISQEELESLNTFKPAKNNAF
                     WPALGSSVLFSVTFRKYMPLFNVRLEKTIVIAIFFVVFLGILFFY"
BASE COUNT           92 a           31 c           40 g          106 t
ORIGIN      
        1 tgaatataat aaataattgg ttagttttat tcattccatt gcttaattgg ttgacaccaa
       61 aaagatacat caagatcagt caagaagaat tagaaagttt aaatactttc aaacctgcta
      121 aaaacaatgc attttggcca gcattaggta gttcagtttt atttagtgtg acttttagaa
      181 agtatatgcc tttatttaat gttagattgg aaaaaacaat agtaatagcc atattttttg
      241 ttgtatttct tggtatttta tttttctac
//
LOCUS       DAHHOJ010000265          263 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.292, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000265 DAHHOJ010000000
VERSION     DAHHOJ010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..263
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.292"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
BASE COUNT           97 a           42 c           47 g           77 t
ORIGIN      
        1 agttggggtg agggccccaa cacagaagct gacgaaaagt cagcttacaa taatgtgcaa
       61 gttggggtgg gacgacgaaa taaattttgc gaaaatatca tttctgtccc actcccatca
      121 aaagaatgac attgaaaaga aatattaaag tttggcgcta taattttctt tctgctcaat
      181 gaaataatac gtttgaaaat tgaattgaat cattcgcgaa attgataatt attctcaatt
      241 aataaaaaat acttattaca cct
//
LOCUS       DAHHOJ010000266          252 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.294, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000266 DAHHOJ010000000
VERSION     DAHHOJ010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 252)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 252)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..252
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.294"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(102..190)
                     /locus_tag="NBH32_002759"
     tRNA            complement(102..190)
                     /locus_tag="NBH32_002759"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(153..155),aa:Ser,seq:gct)
BASE COUNT           85 a           59 c           48 g           60 t
ORIGIN      
        1 tagaatttta aaattgatat aaaagaacag tgaaaatctc attcattata tatataagta
       61 aacaaaaaag cttaaatggt atataccatt taagcaagta acggaaacgg agggattcga
      121 accctcgcgc cgctttcgcg acctacaccc ttagcagggg cgcctcttca gccaacttga
      181 gtacgtttcc aatggctcca caggtaggac tcgaacctac gaccgatcgg ttaacagccg
      241 atagctctac ca
//
LOCUS       DAHHOJ010000267          245 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.295, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000267 DAHHOJ010000000
VERSION     DAHHOJ010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 245)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 245)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..245
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.295"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..>245)
                     /locus_tag="NBH32_002760"
     CDS             complement(<1..>245)
                     /locus_tag="NBH32_002760"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HCD1516273.1"
                     /translation="HTVKVIIETVLLDHDEIVKASELTKAAGADFVKTSTGFAGGGAT
                     AEDVKLMKDTVGADVEVKASGGVRNLEDFNKMVEAGAT"
BASE COUNT           60 a           68 c           33 g           84 t
ORIGIN      
        1 gtcgcacctg cttcaaccat tttattgaaa tcttctaaat tacgtacgcc acctgatgct
       61 tttacttcta catcagcacc tactgtatct ttcattaatt taacgtcttc tgcagtcgca
      121 ccgccacctg caaaacctgt tgaagtttta acgaagtccg caccagccgc ttttgttaat
      181 tcactcgctt ttacaatttc gtcatggtcc aacaataccg tctcaataat cacttttact
      241 gtgtg
//
LOCUS       DAHHOJ010000268          244 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.296, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000268 DAHHOJ010000000
VERSION     DAHHOJ010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 244)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 244)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..244
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.296"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..>244
                     /locus_tag="NBH32_002761"
     CDS             <1..>244
                     /locus_tag="NBH32_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1516274.1"
                     /translation="DDCSACSLRHQCMKPNSKSNKKIMKNYNCEYFKAQVNQKLSEPE
                     TKKIYSQRKIVVESVVGFMKAILGFTRMSVRGINKV"
BASE COUNT           94 a           31 c           45 g           74 t
ORIGIN      
        1 gtgatgactg ttcagcatgt tctttgagac atcaatgcat gaagccaaat tcaaaatcca
       61 ataagaaaat tatgaagaat tataattgtg aatactttaa agcccaagtt aatcaaaagc
      121 tttctgaacc agaaacgaaa aaaatctata gtcaaagaaa aattgttgta gagtctgttg
      181 ttggatttat gaaggctatt ttgggtttca ctcgaatgtc agttcgagga ataaataaag
      241 ttaa
//
LOCUS       DAHHOJ010000269          241 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.297, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000269 DAHHOJ010000000
VERSION     DAHHOJ010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 241)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 241)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..241
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.297"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            1..241
                     /locus_tag="NBH32_002762"
                     /pseudo
     CDS             1..241
                     /locus_tag="NBH32_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_501257.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="E domain-containing protein"
BASE COUNT          104 a           43 c           52 g           42 t
ORIGIN      
        1 taagattgta gagttcggtg gcgagaaaat accgcaaggt cataaagata tcttcgatcc
       61 aaacttacca acagatcaaa cggaaaaagt accaggtaaa ccaggaatta agaatccaga
      121 cacaggaaaa gtgatcgaag agccagtgga tgatgtgatt aaacacggac caaaaacggg
      181 tacaccagaa acaaaaaaca gtagagatac cgtttgaaac aaaacgtgag tttaatccaa
      241 a
//
LOCUS       DAHHOJ010000270          235 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.denovo.299, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000270 DAHHOJ010000000
VERSION     DAHHOJ010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 235)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 235)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..235
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.denovo.299"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..>235)
                     /locus_tag="NBH32_002763"
     CDS             complement(<1..>235)
                     /locus_tag="NBH32_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000277741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCD1516275.1"
                     /translation="EFNDEFICPNNKRIGFKRYAYRNDRYGFKRDFKLYERDDCSACS
                     LRHQCMKPNLKSNKKIMKNYNWEYFKAQINQKLS"
BASE COUNT           72 a           40 c           32 g           91 t
ORIGIN      
        1 agaaagcttt tgattaattt gggctttaaa gtattcccaa ttataattct tcatgatttt
       61 cttattggat ttcaaatttg gcttcatgca ttgatgtctc aaagaacatg ctgaacagtc
      121 atcgcgttca tatagcttga agtcacgttt aaaaccatat ctatcattac ggtacgcata
      181 tcttttaaaa cctattcttt tgttattagg acatataaac tcatcattaa attcg
//
LOCUS       DAHHOJ010000271         5646 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.guided.134, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000271 DAHHOJ010000000
VERSION     DAHHOJ010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.guided.134"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            <1..652
                     /locus_tag="NBH32_002764"
     CDS             <1..652
                     /locus_tag="NBH32_002764"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCD1516276.1"
                     /translation="DMTDFGHRALSDPEFNEQYKEQMKQFKQRILEDDTFAKQFGDLG
                     NVYGKQWRDWVDKDGNHFDQLKTVIEQIKHNPDSRRHIVSAWNPTEIDTMALPPCHTM
                     FQFYVQDGKLSCQLYQRSADIFLGVPYNIASYALLTHLIAKECGLEVGEFVHTFGDAH
                     IYSNHIDAIQTQLARESFNPPTLKINSDKSIFDINYEDLEIVDYESHPAIKAPIAV"
     gene            852..1331
                     /locus_tag="NBH32_002765"
     CDS             852..1331
                     /locus_tag="NBH32_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HCD1516277.1"
                     /translation="MTLSILVAHDLQRVIGFENQLPWHLPNDLKHVKKLSTGHTLVMG
                     RKTFESIGKPLPNRRNVVLTSDTSFNVEGVDVIHSIEDIYQLPGHVFIFGGQTLFEEM
                     IDKVDDMYITVIEGKFRGDTFFPPYTFEDWEVTSSVEGKLDEKNTIPHTFLHLIRKK"
     gene            1346..2185
                     /gene="fakB2"
                     /locus_tag="NBH32_002766"
     CDS             1346..2185
                     /gene="fakB2"
                     /locus_tag="NBH32_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid kinase binding subunit FakB2"
                     /protein_id="HCD1516278.1"
                     /translation="MTKQIIVTDSTSDLSKEYLEANNIHVIPLSLTIEGVSYVDQVDI
                     TSEEFINHIENDEDVKTSQPAIGEFISAYEELGKDGSEIISIHLSSGLSGTYNTAYQA
                     SQMVDANVTVIDSKSISFGLGYQIQHLVELVKEGVSTSEIVKKLNHLRENIKLFVVIG
                     QLNQLIKGGRISKTKGLIGNLMKIKPIGTLDDGRLELVHNARTQNSSIQYLKKEIAEF
                     IGDHEIKSVGVTHANVIEYVDKLKKVFNEAFHVNNYDINVTTPVISAHTGQGAIGLVV
                     LKK"
     gene            2271..2804
                     /gene="msrA"
                     /locus_tag="NBH32_002767"
     CDS             2271..2804
                     /gene="msrA"
                     /locus_tag="NBH32_002767"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCD1516279.1"
                     /translation="MTKEYATLAGGCFWCMVKPFTSYPGIKSVVSGYSGGHVDNPTYE
                     QVCTNQTGHVEAVQITFDPEVTSFENILDIYFKTFDPTDDQGQFFDRGESYQPVIFYH
                     DEHQKKAAEFKKQQLNEQGIFKKPVITPIKPYKNFYPAEDYHQDYYKKNPVHYYQYQR
                     GSGRKAFIESHWGNQNA"
     gene            2797..3225
                     /gene="msrB"
                     /locus_tag="NBH32_002768"
     CDS             2797..3225
                     /gene="msrB"
                     /locus_tag="NBH32_002768"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCD1516280.1"
                     /translation="MLKKDKSELTDIEYIVTQENGTEPPFMNEYWNHFAKGIYVDKIS
                     GKPLFTSEEKFHSECGWPSFSKALDDDEIIELVDKSFGMVRTEVRSEESNSHLGHVFN
                     DGPKESGGLRYCINSAAIQFIPYEKLEELGYGDLISHFDK"
     gene            3237..3737
                     /locus_tag="NBH32_002769"
     CDS             3237..3737
                     /locus_tag="NBH32_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCD1516281.1"
                     /translation="MFKKLFGKGKEVQKDIAIYAPLTGEYVKIEDIPDPVFAQKMMGE
                     GFGINPTEGEVVSPIAGRVDNVFPTKHAIGLKADNGLELLVHIGLDTVQLDGEGFEVL
                     VSSGDEVNVGDPLVRFNLEFINNNAKSVISPIIITNTDQAASINIYDENAVIKGETKV
                     IDVTMN"
     gene            3737..3958
                     /locus_tag="NBH32_002770"
     CDS             3737..3958
                     /locus_tag="NBH32_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HCD1516282.1"
                     /translation="MKNYSFYQFVMTVRGRHDDKGRLAEEIFDDLAFPKHDDDFNILS
                     DYIETHGDFTLPMSVFDDLYEEYTEWLKF"
     gene            4147..5637
                     /locus_tag="NBH32_002771"
     CDS             4147..5637
                     /locus_tag="NBH32_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_499952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HCD1516283.1"
                     /translation="MDDKQHTSSSDDERAEIATSNQDQQTNSSKRVHLKRWQFISILI
                     GTILITAVITVVAYIFINQKISGLNKTDQANLNKIENVYKILNSDYYKKQDSDKLSKA
                     AIDGMVKELKDPYSEYLTKEQTKSFNEGVSGDFVGIGAEMQKKNDQIMVTSPMKGSPA
                     ERAGIRPKDVITKVNGKSIKGKALDEVVKDVRGKENTEVTLTVQRGSEEKDVKIKREK
                     IHVKSVEYKKKDKVGVITINKFQNDTSGELKDAVLKAHKDGLKKIVLDLRNNPGGLLD
                     EAVKMANIFIDKGKTVVKLEKGKDTEAIQTSNDSLKEAKDMDISILVNEGSASASEVF
                     TGALKDYNKAKVYGSKTFGKGVVQTTREFKDGSLLKYTEMKWLTPDGHYIHGKGIKPD
                     VTIDTPKYQSLNVIPNTKTFKVGDDDKNIKTIKIGLSALGYKVDNESTQFDQALENQV
                     KAFQQTNKLEVTGEFNKETNNKFTELLVEKANKHDDVLDKLINILK"
BASE COUNT         2119 a          801 c         1024 g         1702 t
ORIGIN      
        1 agatatgaca gatttcggac atcgtgcatt gagtgatcct gaatttaacg aacaatataa
       61 agaacaaatg aaacaattta agcaacgtat tcttgaagat gatacatttg cgaagcaatt
      121 cggtgattta ggaaatgttt atggtaaaca atggcgagat tgggttgata aagatggtaa
      181 tcattttgat caacttaaaa cagtaattga acaaattaag cataatccag attcaagacg
      241 acacatcgta tctgcatgga atccaacaga aattgataca atggcacttc cgccttgtca
      301 tacaatgttc cagttttatg tccaagatgg taagttaagt tgccagttat accaacgtag
      361 cgcagatatc tttttaggtg tgccatataa tatcgcaagc tacgctttat tgacacacct
      421 tattgccaaa gaatgtggac ttgaagtagg tgaatttgtg catacatttg gagatgcaca
      481 tatttattca aatcatattg atgcaattca aactcaatta gcgcgtgaaa gtttcaatcc
      541 tccaacatta aaaattaaca gtgacaagtc tattttcgac ataaattatg aagatttgga
      601 aattgttgac tatgaatcgc atccagcaat aaaagctcca atagcagtgt agtcattgca
      661 tagttagcta accatataga catcaaaatg acatcatagt attttcaagt gcaaaaaagt
      721 acttttttgt gttaaacgtt ttcataaatt atgcaaaatc attatttcta tcacacttta
      781 tgataaaaat tgtgttaaat taaagataac ttagtaataa aaaatgaaat gatagaagaa
      841 ggaggataat tatgacttta tccattctag ttgcacatga cttgcaacga gtaattggtt
      901 ttgaaaatca attaccttgg cacctaccaa atgatttgaa gcatgttaaa aaactatcaa
      961 caggtcatac tttagtaatg ggtcgtaaga catttgaatc gattggtaaa ccactaccga
     1021 atcgtcgaaa tgttgtactt acttcagata caagtttcaa cgtagagggc gttgatgtga
     1081 ttcattctat tgaagatatt taccaactac cgggacatgt tttcatattt ggagggcaaa
     1141 cattatttga agaaatgatt gataaagtgg acgacatgta tattactgtt attgaaggta
     1201 aattccgtgg tgatacgttc ttcccacctt atacatttga agactgggaa gttacctctt
     1261 cagttgaagg taaactagat gagaaaaata caattccaca tacctttcta catttaattc
     1321 gtaaaaaata agggggaaaa cgaccatgac aaaacagatt atagtaacag actcaacatc
     1381 cgatttatct aaagaatatt tagaagcaaa caacattcat gtaattcctt taagtttaac
     1441 tattgaagga gtttcatacg ttgaccaagt agatattaca tcagaagaat ttattaatca
     1501 tattgaaaat gatgaagatg taaagacaag tcagccagcc ataggtgaat ttatatctgc
     1561 ttatgaagaa ctaggaaaag atggctccga aatcataagt attcatcttt cttcaggatt
     1621 aagtggtaca tataacactg cttaccaagc aagtcaaatg gtagatgcta atgtaactgt
     1681 tattgattca aaatctattt cttttggttt agggtatcaa atacaacacc tagtagagct
     1741 tgtaaaagaa ggtgtctcaa cttctgaaat agttaaaaag ttaaatcatt taagagaaaa
     1801 cattaaatta tttgtagtta tagggcaatt gaatcaatta attaaaggtg gcagaattag
     1861 taaaacaaaa ggtttgattg gtaatcttat gaaaatcaaa ccaattggta cactagatga
     1921 tggtcgctta gagcttgtgc acaatgcgag aactcaaaat tctagtatcc aatacttgaa
     1981 aaaggaaatt gctgaattta taggagatca tgaaatcaaa tccgttggtg tcacacatgc
     2041 taacgttatt gaatatgttg ataaattgaa gaaagttttt aatgaagctt ttcatgtgaa
     2101 taattacgat ataaatgtaa ctacaccagt tatttctgca catactggtc aaggtgcgat
     2161 tggcctcgta gtccttaaga agtaaattta atcttttcag tgttaattac ttccatttca
     2221 atcctttata gactaaattt ataattagat agatagagga ggtaattcat atgacaaaag
     2281 aatatgcaac attagcagga ggatgtttct ggtgcatggt taaaccattt acatcatatc
     2341 caggcatcaa gtcagtcgta tctggttata gtggcggtca tgttgacaac ccaacttatg
     2401 aacaggtatg tacgaatcaa accggccatg tcgaagcagt acaaattacg tttgatccag
     2461 aggttacttc ctttgaaaat atattagaca tatatttcaa aacatttgac ccaaccgatg
     2521 atcaagggca atttttcgat agaggcgaaa gctatcaacc agtcattttc tatcatgatg
     2581 agcatcagaa aaaggctgct gagtttaaaa agcaacaatt aaatgaacaa ggtattttca
     2641 agaaaccagt gattacacct attaaaccat ataaaaattt ctatccagct gaagactacc
     2701 atcaagatta ttacaaaaag aacccggtac attattacca atatcaacgt ggttcaggta
     2761 gaaaagcgtt tatagaatca cattggggga atcaaaatgc ttaaaaaaga taaaagcgaa
     2821 ctaacagata tagaatatat tgttacacaa gaaaatggta ctgaaccacc atttatgaat
     2881 gaatattgga atcattttgc taaagggatt tacgtagata agatttctgg taaaccttta
     2941 tttacatctg aagaaaagtt tcattctgag tgcggatggc ctagcttttc caaagcgctt
     3001 gatgacgatg aaattataga attagtcgac aaatcatttg gtatggtaag aactgaagtc
     3061 cgttcagaag aatcaaatag tcatttagga cacgtcttta atgatggacc taaagaaagt
     3121 ggcggcttaa gatactgtat caattccgct gcaattcaat ttattccata tgaaaaatta
     3181 gaagaattgg gttatggcga tttaatatca cattttgata agtaggagtg gaaagtatgt
     3241 ttaaaaaatt attcggaaaa ggcaaagaag ttcagaaaga tatcgcaatt tatgcaccat
     3301 taactggaga atacgtgaaa attgaagata ttccagatcc tgtattcgca caaaaaatga
     3361 tgggcgaagg ttttggtatt aatccaactg aaggagaagt tgtgtctcca attgcaggac
     3421 gtgttgacaa tgtctttcca actaagcatg cgattgggtt aaaagcagat aacggattag
     3481 aattattagt tcatatcggt ttagacacag ttcaattaga cggtgaagga tttgaagtgt
     3541 tagtgtctag tggtgacgaa gttaacgtcg gagatccatt agtaagattc aaccttgaat
     3601 ttattaataa taacgctaaa tctgttattt caccaattat aattacaaac actgatcaag
     3661 cagcttcaat taatatttat gatgaaaatg ctgtgattaa aggtgaaaca aaagtgattg
     3721 atgtgacaat gaactaatga aaaactattc gttttatcaa tttgtcatga cagttcgtgg
     3781 tcgacacgac gataaaggtc gtctagcaga agagatattt gacgatcttg ctttcccaaa
     3841 acacgatgat gattttaaca tactgtctga ttatattgag acacatggtg atttcacatt
     3901 gccaatgtct gtatttgatg atttatatga agaatatacg gaatggctaa aattttaaca
     3961 taatatttaa taatagttag aaccagggtg atgcaatttg ttatcctggt tttaatttaa
     4021 aataaactaa gttgtgacta aaaattaatc aattatagtg aaatatggtg cgctatcttg
     4081 cataaattga tatgattaac tacacagaat ttaaaagtac ataatacata ataaggaagt
     4141 gatacaatgg atgataagca acacacatct tcatccgatg atgaacgcgc tgaaattgca
     4201 acaagcaatc aagaccaaca aactaattca tcgaaacgcg ttcacttaaa acgttggcaa
     4261 ttcatatcaa ttttaattgg tacaatccta ataacagctg tcatcacagt tgttgcatat
     4321 atttttataa atcaaaaaat aagtggttta aacaaaactg atcaagcaaa cttaaataaa
     4381 attgaaaatg tgtataaaat cttaaatagt gattattaca aaaaacagga ctctgacaag
     4441 ttaagtaaag ctgcaattga tggcatggtc aaagaattaa aagatcctta ttctgaatat
     4501 ttaacaaaag aacaaacgaa atcctttaat gaaggtgttt caggtgattt tgtaggtatt
     4561 ggtgcagaaa tgcaaaagaa aaatgatcaa attatggtta ctagtcctat gaagggatct
     4621 ccagcagaac gtgctggcat tcgtcctaaa gatgtcatta ctaaagtaaa tggaaaatca
     4681 attaaaggta aagcattaga tgaagttgtc aaagatgttc gtggtaaaga aaacactgaa
     4741 gtaactttaa ctgttcaacg aggcagtgaa gaaaaagacg ttaagattaa acgtgaaaaa
     4801 attcatgtta aaagtgttga gtataagaaa aaagataagg ttggagttat tactattaat
     4861 aaattccaga atgatacatc aggtgaattg aaagatgcag ttcttaaagc tcacaaagat
     4921 ggtttgaaaa agattgtttt agatttaaga aataatcctg gtggactact agatgaagct
     4981 gttaaaatgg caaatatttt tatcgataaa ggaaaaactg ttgttaaact agaaaaaggt
     5041 aaagatactg aagcaattca aacttctaat gactcgttaa aagaagcgaa agacatggat
     5101 atatccatct tagtgaatga aggttcagct agcgcttctg aagtatttac tggtgcgcta
     5161 aaggactata ataaagctaa agtttatggg tcaaaaacat tcggtaaagg tgtcgtacaa
     5221 actacaagag agtttaagga cggttcattg ttaaaatata ctgaaatgaa atggttaacg
     5281 ccagatggtc attatattca cggtaaaggc attaaaccag acgttactat tgacacacct
     5341 aaatatcaat ctttaaatgt cattcctaat acgaaaacat ttaaagttgg agacgatgat
     5401 aaaaatatta aaactattaa aattggttta tcagctttag gttataaagt tgataatgaa
     5461 tcaacgcaat ttgatcaagc tttagaaaat caagttaaag ctttccaaca aacgaataaa
     5521 ctcgaggtaa ctggtgaatt taataaagaa acgaataata aatttactga gttattagtt
     5581 gaaaaagcta ataaacatga tgatgttctc gataaattga ttaatatttt aaaataagtg
     5641 atacac
//
LOCUS       DAHHOJ010000272         4255 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.guided.155, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000272 DAHHOJ010000000
VERSION     DAHHOJ010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 4255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4255
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.guided.155"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            96..695
                     /locus_tag="NBH32_002772"
     CDS             96..695
                     /locus_tag="NBH32_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HCD1516284.1"
                     /translation="MAFKLPNLPYAYDALEPYIDQRTMEFHHDKHHNTYVTKLNATVE
                     GTELEHQSLADMIANLDKVPEAMRMSVRNNGGGHFNHSLFWEILSPNSEEKGGVIDDI
                     KAQWGTLDEFKNEFANKATTLFGSGWTWLVVNDGKLEIVTTPNQDNPLTEGKTPILLF
                     DVWEHAYYLKYQNKRPDYMTAFWNIVNWKKVDELYQAAK"
     gene            complement(1038..1793)
                     /locus_tag="NBH32_002773"
     CDS             complement(1038..1793)
                     /locus_tag="NBH32_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCD1516285.1"
                     /translation="MEVVYVTQTQPKFLTIYNTLYKEIQIGKYPSGHALPTEKELCAR
                     FDVSRMTLRQAIKLLAEDGIVESTRGKGHFVIPQTNAQHASSMDQFKHPLDQILLDRV
                     SMSSINYRVDLESEYTNHLFANHPSAVIAMERYYQKKDNHSKQADAFCFTFIPLNVID
                     TFKVNTQNEDDMKTFVEQTIYSNAYQSDLKMSITKAPHFKNHSHVFDGDTHCWLIIET
                     LYAQTPYPIMINKWYIPQEISELTLTRIRQSDY"
     gene            2029..2736
                     /gene="deoD"
                     /locus_tag="NBH32_002774"
     CDS             2029..2736
                     /gene="deoD"
                     /locus_tag="NBH32_002774"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCD1516286.1"
                     /translation="MKSTPHIKPMNDVEIAETVLLPGDPLRAKFIAETYLDDVEQFNT
                     VRNMFGFTGTYKGKKVSVMGSGMGMPSIGIYSYELIHTFGCKKLIRVGSCGAMQENID
                     LYDVIIAQGASTDSNYVQQYQLPGHFAPIASYQLLEKAVETARDKGVRHHVGNVLSSD
                     IFYNADTTASERWMRMGILGVEMESAALYMNAIYAGVEALGVFTVSDHLIHETSTTPE
                     ERERAFTDMIEIALSLV"
     gene            2743..4095
                     /gene="tet(38)"
                     /locus_tag="NBH32_002775"
     CDS             2743..4095
                     /gene="tet(38)"
                     /locus_tag="NBH32_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(38)"
                     /protein_id="HCD1516287.1"
                     /translation="MNVEYSKIKKAVPILLFLFVFSLVIDNSFKLISVAIADDLNISV
                     TTVSWQATLAGLVIGIGAVVYASLSDAISIRTLFIYGVILIIIGSIIGYIFQHQFALL
                     LVGRIIQTAGLAAAETLYVIYVAKYLSKEDQKTYLGLSTSSYSLSLVIGTLSGGFIST
                     YLHWTNMFLIALIVVFTLPFLFKLLPKENNTNKAHLDFVGLILVATIATTVMLFITNF
                     NWLYMIGALIAIIVFALYIKNAQRPLVNKSFFQNKRYASFLFIVFVMYAIQLGYIFTF
                     PFIMEQIYHLQLDTTSLLLVPGYIVAVIVGALSGKIGEYLNSKQAIITAIILIALSLI
                     LPAFAVGNHISIFVISMIFFAGSFALMYAPLLNEAIKTIDLNMTGVAIGFYNLIINVA
                     VSVGIAIAAALIDFKALNFPGNDALSSHFGIILIILGLMSIVGLVLFVILNRWTQSEK
                     "
     gene            4176..>4255
                     /locus_tag="NBH32_002776"
     CDS             4176..>4255
                     /locus_tag="NBH32_002776"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_498700.1"
                     /note="catalyzes the formation of D-glyceraldehyde
                     3-phosphate and acetaldehyde from
                     2-deoxy-D-ribose-5-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-deoxyribose-5-phosphate aldolase"
                     /protein_id="HCD1516288.1"
                     /translation="MKFEKYIDHTLLKSESTRTQIDQIID"
BASE COUNT         1421 a          643 c          755 g         1436 t
ORIGIN      
        1 atataattgg atataacaaa taaataataa ttattgcaaa acacacccga aattaattat
       61 tataaaagta tattcataaa aggaggaata tacttatggc atttaaatta ccaaatttac
      121 catatgcata tgatgcattg gaaccatata tagatcaaag aacaatggag tttcatcacg
      181 acaaacatca caatacgtac gtgacgaaat taaacgcaac agttgaagga acagagttag
      241 agcatcaatc actagcggat atgattgcta acttagacaa ggtaccggaa gcgatgagga
      301 tgtcagtccg taataatggc ggtggtcatt ttaaccattc attattctgg gaaatactat
      361 cacctaattc tgaagaaaaa ggtggcgtaa tagacgacat taaagcgcag tggggcactt
      421 tagatgaatt taaaaatgaa tttgcaaata aagcaacaac attatttgga tcaggttgga
      481 cttggttagt tgttaatgat ggaaaattag aaattgtgac aacgccaaac caagataatc
      541 cattaacaga aggcaaaaca ccaatcttac tatttgatgt ttgggagcat gcctactatc
      601 tgaaatatca aaataaacgt ccagactata tgactgcatt ttggaatatt gttaactgga
      661 aaaaagttga tgaattatac caagcagcaa aataatataa cttaatataa ttgaggtgga
      721 gcatctacaa ggtgtttcac cttttttgta tgtatgaggt aatcaatttg ctttttgtat
      781 aatgggttgg agaatcaaat gtaggtaaaa cagtacaatc atttacaatg atgaatactt
      841 tacttgtgtt ctctgtgctt gtgaactcaa tgtttcgaga agagcgcaca tcgaataaca
      901 aaattattta gacaacgttc aacaaataaa taaacatcat acgttttaaa gtagaaaaaa
      961 tcatgtgatt acctgaaagt tattgtcgat taaacaatag ctaaacagtc atcacatgat
     1021 tttttatttt aataatttta ataatccgat tgtcttatac gtgtcagtgt taattcagat
     1081 atttcctgtg gaatatacca cttattaatc ataattggat aaggtgtttg tgcgtacaat
     1141 gtttcaataa tcagccaaca atgtgtatca ccatcaaaca cgtgactatg atttttgaag
     1201 tggggcgctt tggtaataga catttttaaa tctgattgat atgcattgct ataaatcgtc
     1261 tgctcaacga acgtcttcat gtcgtcttcg ttttgtgtat tcactttaaa tgtgtcaatg
     1321 acatttaacg gtataaaggt aaaacaaaat gcatcagctt gcttagaatg attgtccttt
     1381 ttttgataat agcgttccat tgcaatgacg gcagaaggat ggtttgcaaa caaatgattt
     1441 gtatattcac tttctaaatc aacacgataa ttaattgatg acatagatac acgatctagc
     1501 aatatttgat caagtggatg cttaaattga tccatacttg aagcgtgttg ggcatttgtt
     1561 tgtggaataa cgaagtgtcc cttccctctt gtactctcta cgatgccatc ttcggccaac
     1621 aattttatag cttggcgcaa agtcatacga ctgacatcaa agcgcgcaca aagttccttt
     1681 tcagtaggta atgcatggcc actcggatat tttcctattt gaatttcttt atataacgta
     1741 ttataaatcg ttaaaaattt tggttgtgtt tgcgtcacgt agacaacctc cataaagtta
     1801 cttaatcact ctcatcatac aataattttt actcgaattg gaaaaattat aaaaattaaa
     1861 catagatagg ctttgaaaat tagttttata caaggttaga agctataact gtaaaatgtt
     1921 cttaatattg tcaaaatgta atgcttgaaa gcgcttttaa aaaatattat tatatacatg
     1981 gttagacaaa tagacaaatc actatacaaa tattgggagg aatattttat gaaatcaaca
     2041 ccacacatta agccaatgaa tgacgtcgaa attgcagaaa cggtgctatt gccaggagat
     2101 ccgttaagag ctaagttcat tgcagaaaca tatttggacg atgtggaaca gttcaataca
     2161 gtgcgaaaca tgtttggttt taccggaaca tataaaggta aaaaagtttc tgtcatgggt
     2221 tcaggtatgg gtatgccatc tattggcatt tactcttatg aattaattca tacatttggt
     2281 tgtaaaaaat taattcgcgt tggttcttgt ggcgcgatgc aagaaaacat tgatttatat
     2341 gatgtgatta ttgcacaagg tgcctctact gattcaaatt acgttcaaca atatcaatta
     2401 ccaggtcatt ttgcgccaat tgcttcttat cagttattag aaaaagcagt tgaaacagca
     2461 cgtgacaaag gtgtacgtca tcatgtaggt aatgtgttat caagtgatat tttctataac
     2521 gcggatacaa cagcgagtga acgttggatg cgtatgggta ttttaggtgt agaaatggaa
     2581 tcagctgcat tatacatgaa tgcaatttac gctggtgtcg aagcattagg tgtgttcaca
     2641 gtgagcgatc atttaattca tgaaacgtca acaacacctg aggaaaggga acgtgcattt
     2701 acagatatga ttgaaattgc actgtcattg gtgtagatga ttatgaatgt tgaatattct
     2761 aaaataaaga aagcagtacc tattttatta ttcttatttg tattcagttt ggttatagac
     2821 aactcattca aattgatttc tgtagccatt gctgatgact taaacatttc tgtaactaca
     2881 gtaagttggc aagcgacatt agccggttta gtaattggta ttggcgctgt agtatacgct
     2941 tcattatctg atgccattag tatacgcaca ctatttattt atggcgtgat attaatcatt
     3001 atcggatcaa ttattggtta cattttccaa catcaattcg cattactttt agttggacgt
     3061 attattcaaa ctgcaggttt agctgctgca gagacattat atgtgatata tgttgcaaag
     3121 tatctttcta aagaggacca gaagacttac cttggcttaa gtacgagcag ttattccttg
     3181 tcattagtta tcggtacatt atcaggtgga tttatttcta cgtatttaca ctggacaaat
     3241 atgtttttaa ttgcattaat cgtagtattt acgttgccat tcctatttaa attattacca
     3301 aaagaaaata atacaaataa agctcattta gattttgttg gcttaattct agtggcaact
     3361 attgctacaa cagtcatgct gtttattacg aactttaatt ggttatatat gattggtgct
     3421 ttaattgcga ttatcgtttt tgcgctatat attaaaaatg cgcaacgtcc attagtaaac
     3481 aaatcatttt tccaaaataa acgttatgct tcatttttat ttatagtatt cgtaatgtat
     3541 gctatccaat tgggttatat ttttacgttc ccattcataa tggaacaaat ttatcatctt
     3601 caactagaca caacatcact gttattagta ccgggttata ttgtagcagt catcgttggt
     3661 gcactaagtg gtaaaatcgg cgaatatctt aattcaaaac aagcgattat cacagcaatt
     3721 attttaatcg cactgagctt aattttacct gcatttgcag taggtaatca catttcaatc
     3781 ttcgtcattt ctatgatatt ctttgcaggt agctttgctt taatgtatgc acctttactt
     3841 aacgaagcca ttaaaacaat agatcttaat atgacaggtg tggctattgg tttttataat
     3901 ttaattatta atgtggcggt atctgtaggt attgcgattg ctgcggctct aatcgatttt
     3961 aaagcattaa atttcccagg caatgatgca ttaagttcac atttcggtat tattttaatt
     4021 attttaggtt taatgagtat tgtcggatta gttttattcg tcatcttaaa tcgttggaca
     4081 caatctgaaa aataaataga tataaattcg cgagatatat tcgtatttat agtaaaatta
     4141 aataaagaga ttatataaca cgaggagtag taagtatgaa atttgagaaa tatatagatc
     4201 acactttatt gaagtctgag tcaacacgta cgcaaatcga tcaaatcatc gatga
//
LOCUS       DAHHOJ010000273         3817 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.guided.161, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000273 DAHHOJ010000000
VERSION     DAHHOJ010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.guided.161"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            141..2150
                     /gene="mecA"
                     /locus_tag="NBH32_002777"
     CDS             141..2150
                     /gene="mecA"
                     /locus_tag="NBH32_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP2a family beta-lactam-resistant peptidoglycan
                     transpeptidase MecA"
                     /protein_id="HCD1516289.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMTERPIKIYNSLGVKDINIQDRKIKKVSKNKKRVDAQYKI
                     KTNYGNIDRNVQFNFVKEDGMWKLDWDHSVIIPGMQKDQSIHIENLKSERGKILDRNN
                     VELANTGTAYEIGIVPKNVSKKDYKAIAKELSISEDYIKQQMDQNWVQDDTFVPLKTV
                     KKMDEYLSDFAKKFHLTTNETESRNYPLEKATSHLLGYVGPINSEELKQKEYKGYKDD
                     AVIGKKGLEKLYDKKLQHEDGYRVTIVDDNSNTIAHTLIEKKKKDGKDIQLTIDAKVQ
                     KSIYNNMKNDYGSGTAIHPQTGELLALVSTPSYDVYPFMYGMSNEEYNKLTEDKKEPL
                     LNKFQITTSPGSTQKILTAMIGLNNKTLDDKTSYKIDGKGWQKDKSWGGYNVTRYEVV
                     NGNIDLKQAIESSDNIFFARVALELGSKKFEKGMKKLGVGEDIPSDYPFYNAQISNKN
                     LDNEILLADSGYGQGEILINPVQILSIYSALENNGNINAPHLLKDTKNKVWKKNIISK
                     ENINLLTDGMQQVVNKTHKEDIYRSYANLIGKSGTAELKMKQGETGRQIGWFISYDKD
                     NPNMMMAINVKDVQDKGMASYNAKISGKVYDELYENGNKKYDIDE"
     gene            complement(2196..2624)
                     /locus_tag="NBH32_002778"
     CDS             complement(2196..2624)
                     /locus_tag="NBH32_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000872606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HCD1516290.1"
                     /translation="MKYDDFIVGETFKTKSLHITEEEIIQFATTFDPQYMHIDKEKAE
                     QSRFKGIIASGMHTLSISFKLWVEEGKYGEEVVAGTQMNNVKFIKPVYPGNTLYVIAE
                     ITNKKSIKKENGLVTVSLSTYNENEEIVFKGEVTALINNS"
     gene            complement(2721..3464)
                     /locus_tag="NBH32_002779"
     CDS             complement(2721..3464)
                     /locus_tag="NBH32_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001830227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphoryl diester phosphodiesterase"
                     /protein_id="HCD1516291.1"
                     /translation="MLTVYGHRGLPSKAPENTIASFKAASEVEGINWLELDVAITKDE
                     QLIIIHDDYLERTTNMSGEITELNYDEIKDASAGSWFGEKFKDEHLPTFDDVVKIANE
                     YNMNLNVELKGITGPNGLALSKSMVKQVEEQLTNLNQNQEVLISSFNVVLVKLAEEIM
                     PQYNRAVIFHTTSFREDWRTLLDYCNAKIVNTEDAKLTKAKVKMVKEAGYELNVWTVN
                     KPARANQLANWGVDGIFTDNADKMVHLSQ"
BASE COUNT         1384 a          606 c          572 g         1255 t
ORIGIN      
        1 agcaatgaac tgattatact taacattaaa aaagatgata acaccttcta cacctccata
       61 tcacaaaaat tataacatta ttttgacata aatactacat ttgtaatata ctacaaatgt
      121 agtcttatat aaggaggata ttgatgaaaa agataaaaat tgttccactt attttaatag
      181 ttgtagttgt cgggtttggt atatattttt atgcttcaaa agataaagaa attaataata
      241 ctattgatgc aattgaagat aaaaatttca aacaagttta taaagatagc agttatattt
      301 ctaaaagcga taatggtgaa gtagaaatga ctgaacgtcc gataaaaata tataatagtt
      361 taggcgttaa agatataaac attcaggatc gtaaaataaa aaaagtatct aaaaataaaa
      421 aacgagtaga tgctcaatat aaaattaaaa caaactacgg taacattgat cgcaacgttc
      481 aatttaattt tgttaaagaa gatggtatgt ggaagttaga ttgggatcat agcgtcatta
      541 ttccaggaat gcagaaagac caaagcatac atattgaaaa tttaaaatca gaacgtggta
      601 aaattttaga ccgaaacaat gtggaattgg ccaatacagg aacagcatat gagataggca
      661 tcgttccaaa gaatgtatct aaaaaagatt ataaagcaat cgctaaagaa ctaagtattt
      721 ctgaagacta tatcaaacaa caaatggatc aaaattgggt acaagatgat accttcgttc
      781 cacttaaaac cgttaaaaaa atggatgaat atttaagtga tttcgcaaaa aaatttcatc
      841 ttacaactaa tgaaacagaa agtcgtaact atcctctaga aaaagcgact tcacatctat
      901 taggttatgt tggtcccatt aactctgaag aattaaaaca aaaagaatat aaaggctata
      961 aagatgatgc agttattggt aaaaagggac tcgaaaaact ttacgataaa aagctccaac
     1021 atgaagatgg ctatcgtgtc acaatcgttg acgataatag caatacaatc gcacatacat
     1081 taatagagaa aaagaaaaaa gatggcaaag atattcaact aactattgat gctaaagttc
     1141 aaaagagtat ttataacaac atgaaaaatg attatggctc aggtactgct atccaccctc
     1201 aaacaggtga attattagca cttgtaagca caccttcata tgacgtctat ccatttatgt
     1261 atggcatgag taacgaagaa tataataaat taaccgaaga taaaaaagaa cctctgctca
     1321 acaagttcca gattacaact tcaccaggtt caactcaaaa aatattaaca gcaatgattg
     1381 ggttaaataa caaaacatta gacgataaaa caagttataa aatcgatggt aaaggttggc
     1441 aaaaagataa atcttggggt ggttacaacg ttacaagata tgaagtggta aatggtaata
     1501 tcgacttaaa acaagcaata gaatcatcag ataacatttt ctttgctaga gtagcactcg
     1561 aattaggcag taagaaattt gaaaaaggca tgaaaaaact aggtgttggt gaagatatac
     1621 caagtgatta tccattttat aatgctcaaa tttcaaacaa aaatttagat aatgaaatat
     1681 tattagctga ttcaggttac ggacaaggtg aaatactgat taacccagta cagatccttt
     1741 caatctatag cgcattagaa aataatggca atattaacgc acctcactta ttaaaagaca
     1801 cgaaaaacaa agtttggaag aaaaatatta tttccaaaga aaatatcaat ctattaactg
     1861 atggtatgca acaagtcgta aataaaacac ataaagaaga tatttataga tcttatgcaa
     1921 acttaattgg caaatccggt actgcagaac tcaaaatgaa acaaggagaa actggcagac
     1981 aaattgggtg gtttatatca tatgataaag ataatccaaa catgatgatg gctattaatg
     2041 ttaaagatgt acaagataaa ggaatggcta gctacaatgc caaaatctca ggtaaagtgt
     2101 atgatgagct atatgagaac ggtaataaaa aatacgatat agatgaataa caaaacagtg
     2161 aagcaatccg taacgatggt tgcttcactg ttttattatg aattattaat aagtgctgtt
     2221 acttctccct taaatacaat ttcttcattt tcattgtatg ttgaaagtga cactgtaacg
     2281 agtccatttt ctttttttat ggatttctta tttgtaattt cagcgataac gtacaatgta
     2341 ttacctgggt atacaggttt aataaattta acgttattca tttgtgttcc tgctacaact
     2401 tcttctccgt atttaccttc ttctacccat aatttaaatg atattgaaag tgtatgcatg
     2461 ccagatgcaa tgataccttt aaatctactt tgttctgctt tttctttatc tatatgcata
     2521 tattgaggat caaaagttgt tgcaaattgg ataatttctt cttctgtaat atgaaggctt
     2581 tttgttttga atgtttctcc tactataaaa tcatcgtatt tcatatatgt ctctctttct
     2641 tattcaaatt aattttttag tatgtaacat gttaaaggta agtctaccgt cactgaaacg
     2701 taagactcac ctctaacttt ctattgagac aaatgcacca ttttatctgc attgtctgta
     2761 aagataccat caactcccca attagcaagt tggtttgcac gtgctggttt gtttacagtc
     2821 catacgttca attcataacc cgcttctttt accattttta cttttgcttt agtaagtttg
     2881 gcatcttcag tgtttactat tttagcatta cagtaatcta aaagtgttct ccagtcttca
     2941 cgaaacgaag ttgtatggaa tataactgct ctgttatatt gtggcatgat ttcttctgca
     3001 agtttaacaa gcacaacatt aaagcttgaa atgagcactt cttgattctg atttaagttt
     3061 gttaattgtt cttccacttg cttaaccata cttttagaaa gtgctagtcc attcggtcca
     3121 gtaatacctt ttaattctac atttaaattc atattatatt catttgctat ttttactaca
     3181 tcatcgaaag ttggcaaatg ttcatctttg aatttttcac caaaccaaga tcctgcagaa
     3241 gcatctttaa tttcatcata attcaattca gttatttccc cggacatatt tgtagtccgt
     3301 tctaaataat catcatgaat gataatcagt tgttcatctt ttgtaattgc aacatctaac
     3361 tccaaccagt ttataccttc tacttctgaa gcagctttaa atgatgcaat tgtattttcc
     3421 ggagctttac taggtaatcc tctatgtcca tatacagtta gcatattacc tctccttgca
     3481 tttttatttt tttaattaac gtaactgtat tatcacatta atcgcacttt tatttccatt
     3541 aaaaagagat gaatatcata aataaagaag tcgatagatt cgtattgatt atggagttaa
     3601 tctacgtctc atctcatttt taaaaaatca tttatgtccc aagctccatt ttgtaatcaa
     3661 gtctagtttt tctgtacccc ttatctgcaa ttttacttag gattgctttt aacttacccc
     3721 ttatcagcaa ttttactgag aactgctttt aacgcacctc ttatctgcaa ttttgcctag
     3781 aactgctttt aacgtacctc ttatctgcaa ttttact
//
LOCUS       DAHHOJ010000274         1753 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.guided.210, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000274 DAHHOJ010000000
VERSION     DAHHOJ010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1753
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.guided.210"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            complement(<1..194)
                     /locus_tag="NBH32_002780"
     CDS             complement(<1..194)
                     /locus_tag="NBH32_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001801873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HCD1516292.1"
                     /translation="MMKKIKIVPLILIVVVVGFGIYFYASKDKEINNTIDAIEDKNFK
                     QVYKDSSYISKSDNGEVEMT"
     gene            291..1277
                     /locus_tag="NBH32_002781"
     CDS             291..1277
                     /locus_tag="NBH32_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HCD1516293.1"
                     /translation="MLSSFLMLSIISSLLTIYVIFLVRMLYIKYTQNIMSHKIWLLVL
                     VSTLIPLIPFYKISNFTFSKDMMNRNVSDTTSSVSHMLDGQQSSVTKDLAINVNQFET
                     SNITYMILLIWVFGSLLCLFYMIKAFRQIDVIKSSSLESSYLNERLKVCQSKMQFYKK
                     HITISYSSNIDNPMVFGLVKSQIVLPTVVVETMNDKEIEYIILHELSHVKSHDLIFNQ
                     LYVVFKMIFWFNPALYISKTMMDNDCEKVCDRNVLKILNRHEHIRYGESILKCSILKS
                     QHINNVAAQYLLGFNSNIKERVKYIALYDSMPKPNRNKRIVAYIVCSISSFT"
     gene            1249..1509
                     /locus_tag="NBH32_002782"
     CDS             1249..1509
                     /locus_tag="NBH32_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001549961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3387 domain-containing protein"
                     /protein_id="HCD1516294.1"
                     /translation="MYVVYRASHETAKEALGDKELRAIAHELTKTVKDNMSVDWSKRD
                     SAKAKMRVQVRRLLKKYGYPPDLQKMAVEQVVEQAELMASQQ"
     gene            complement(1500..>1753)
                     /locus_tag="NBH32_002783"
     CDS             complement(1500..>1753)
                     /locus_tag="NBH32_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011274780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HCD1516295.1"
                     /translation="VFGFMKAILGFTRMSVRGLNKVKRELGFVLMALNIRKVVAQRAE
                     NNQKIYKKDNFYIISIEIVFFHLSKNFMSRTHYDLFFYC"
BASE COUNT          628 a          249 c          269 g          607 t
ORIGIN      
        1 tcagtcattt ctacttcacc attatcgctt ttagaaatat aactgctatc tttataaact
       61 tgtttgaaat ttttatcttc aattgcatca atagtattat taatttcttt atcttttgaa
      121 gcataaaaat atataccaaa cccgacaact acaactatta aaataagtgg aacaattttt
      181 atctttttca tcaatatcct ccttatataa gactacattt gtagtatatt acaaatgtag
      241 tatttatgtc aaaataatgt tataattttt gtgatatgga ggtgtagaag gtgttatcat
      301 cttttttaat gttaagtata atcagttcat tgctcacgat atatgtaatt tttttagtga
      361 gaatgctcta tataaaatat actcaaaata ttatgtcaca taagatttgg ttattagtgc
      421 tcgtctccac gttaattcca ttaataccat tttacaaaat atcgaatttt acattttcaa
      481 aagatatgat gaatcgaaat gtatctgaca cgacttcttc ggttagtcat atgttagatg
      541 gtcaacaatc atctgttacg aaagacttag caattaatgt taatcagttt gagacctcaa
      601 atataacgta tatgattctt ttgatatggg tatttggtag tttgttgtgc ttattttata
      661 tgattaaggc attccgacaa attgatgtta ttaaaagttc gtcattggaa tcgtcatatc
      721 ttaatgaacg acttaaagta tgtcaaagta agatgcagtt ctacaaaaag catataacaa
      781 ttagttatag ttcaaacatt gataatccga tggtatttgg tttagtgaaa tcccaaattg
      841 tactaccaac tgtcgtagtc gaaaccatga atgacaaaga aattgaatat attattctac
      901 atgaactatc acatgtgaaa agtcatgact taatattcaa ccagctttat gttgttttta
      961 aaatgatatt ctggtttaat cctgcactat atataagtaa aacaatgatg gacaatgact
     1021 gtgaaaaagt atgtgataga aacgttttaa aaattttgaa tcgccatgaa catatacgtt
     1081 atggtgaatc gatattaaaa tgctctattt taaaatctca gcacataaat aatgtggcag
     1141 cacaatattt actaggtttt aattcaaata ttaaagaacg tgttaagtat attgcacttt
     1201 atgattcaat gcctaaacct aatcgaaaca agcgtattgt tgcgtatatt gtatgtagta
     1261 tatcgagctt cacatgaaac agctaaagaa gctttgggcg ataaagagtt aagagccatt
     1321 gcacatgagt taactaaaac agttaaggat aacatgagtg ttgattggtc taaacgagac
     1381 agtgctaaag ctaaaatgag agttcaagtt agacgcctat taaagaaata tggctatcca
     1441 ccagatcttc aaaaaatggc tgtggaacaa gttgtagagc aagcagaatt aatggcaagt
     1501 cagcaataaa aaaataaatc ataatgagtc cgggacataa agttcttgga taagtgaaaa
     1561 aagacaattt ctattgaaat aatatagaaa ttgtcttttt tataaatttt ttgattattt
     1621 tcagctcgtt gagctactac ttttcttata ttaagtgcca ttaatacaaa accaagttct
     1681 cttttgactt tattgagtcc tcggacagac atccgagtga aacccaaaat agccttcata
     1741 aatccaaaaa cag
//
LOCUS       DAHHOJ010000275         1033 bp    DNA     linear   BCT 30-MAY-2022
DEFINITION  TPA_asm: Staphylococcus aureus isolate M910497
            SAMN28744144-rid14504633.guided.240, whole genome shotgun sequence.
ACCESSION   DAHHOJ010000275 DAHHOJ010000000
VERSION     DAHHOJ010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN28744144
            Sequence Read Archive: SRR19442544
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Staphylococcus aureus
  ORGANISM  Staphylococcus aureus
            Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
            Staphylococcus.
REFERENCE   1  (bases 1 to 1033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (30-MAY-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 30-MAY-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001317514.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001317514.1
            Genome Coverage       :: 179x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 05/30/2022 07:23:37
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 2,784
            CDSs (total)                      :: 2,715
            Genes (coding)                    :: 2,649
            CDSs (with protein)               :: 2,649
            Genes (RNA)                       :: 69
            rRNAs                             :: 4, 3, 3 (5S, 16S, 23S)
            complete rRNAs                    :: 4 (5S)
            partial rRNAs                     :: 3, 3 (16S, 23S)
            tRNAs                             :: 55
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 66
            CDSs (without protein)            :: 66
            Pseudo Genes (ambiguous residues) :: 0 of 66
            Pseudo Genes (frameshifted)       :: 32 of 66
            Pseudo Genes (incomplete)         :: 23 of 66
            Pseudo Genes (internal stop)      :: 25 of 66
            Pseudo Genes (multiple problems)  :: 13 of 66
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1033
                     /organism="Staphylococcus aureus"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN28744144-rid14504633.guided.240"
                     /isolate="M910497"
                     /isolation_source="nasal swab"
                     /host="Homo sapiens"
                     /db_xref="taxon:1280"
                     /country="Australia: Queensland"
                     /collection_date="2019-10-29"
     gene            169..1014
                     /gene="blaZ"
                     /locus_tag="NBH32_002784"
     CDS             169..1014
                     /gene="blaZ"
                     /locus_tag="NBH32_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000733274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-hydrolyzing class A beta-lactamase
                     BlaZ"
                     /protein_id="HCD1516296.1"
                     /translation="MKKLILLIAIALVLSACNSTSSHAKELNNLEKKYNANIGVYALD
                     TKSGKEVKFNADKRFAYASTSKAINSAILLEQVPYNKLNKKVHINKDDIVAYSPILEK
                     YVGKDITLKELIEASMKYSDNTANNKIINEIGGIKKIKKRLKKLGDKVTNPVRYEIEL
                     NYYSPKSKKDTSTPAAFGKTLNKLIANGKLSKKNKNFLLDLMLNNKNGDTLIKDGVPK
                     DYKVADKSGQAITYASRNDVAFIYPKNQSEPIILVIFTNKDNKSDKPNDKLISETAKN
                     VINKF"
BASE COUNT          448 a          123 c          141 g          321 t
ORIGIN      
        1 aataataaca atagaaatat aaaacaaaag cttactatgc tcattattaa taacttagcc
       61 atttcaacac cttctttcaa atatttataa taaactattg acgcgaatat tacaattgta
      121 atattattga tttatagaaa ttacaactgt aatattggag ggtatatttt gaaaaagtta
      181 atacttttaa ttgcaattgc tttagtttta agtgcatgta attcaaccag ttcacatgct
      241 aaagagttaa ataatttaga aaagaaatat aatgctaata ttggtgtcta tgcattagat
      301 actaaaagtg gtaaggaagt aaaatttaat gccgataaga gatttgccta tgcttcaact
      361 tcaaaagcga taaatagtgc tattttgtta gaacaagtac cttataataa gttaaataaa
      421 aaagtacata ttaacaaaga tgatatagtt gcttattctc ctattttaga aaaatatgta
      481 ggaaaagata tcactttaaa agaacttatt gaagcttcaa tgaagtatag tgataataca
      541 gcaaacaata aaattataaa cgaaatcggt ggaatcaaaa aaattaaaaa acgtttaaaa
      601 aaattgggag ataaagtaac aaatccagtt agatatgaaa tagaattaaa ttactattca
      661 ccaaagagca aaaaagatac ttcaacgcct gctgctttcg gcaagacttt aaataaactt
      721 atcgcaaatg gaaaattaag caaaaaaaat aaaaatttct tacttgattt aatgttaaat
      781 aataaaaacg gagacacttt aattaaagat ggtgttccaa aagactataa ggttgctgat
      841 aaaagtggtc aagcaataac atatgcttct agaaatgatg tagcttttat ttatcctaag
      901 aaccaatctg aacctattat tttagtcatt tttacgaata aagacaataa aagtgataaa
      961 cctaatgata aattgataag tgaaaccgcc aagaatgtaa taaacaaatt ttaatagtct
     1021 aaatgtacta caa
//
